LOCUS       DAOQUR010000001       118049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_165_length_118049_cov_10.685806, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000001 DAOQUR010000000
VERSION     DAOQUR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 118049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 118049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118049
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165_length_118049_cov_10.685806"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..582
                     /locus_tag="PKI50_00005"
     CDS             346..582
                     /locus_tag="PKI50_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02133.1"
                     /translation="MANGLGSTGCGCGAPASVSPILDKCKGNGFLLLLLLFVLLASCT
                     TLGREKNFFFIIILAIIAFGGFGRTFGFNNGTAI"
     gene            613..930
                     /locus_tag="PKI50_00010"
     CDS             613..930
                     /locus_tag="PKI50_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02134.1"
                     /translation="MVDGVAGNAFGGGNFDLLQDSIGETVTIFTTSGGLSGCGFTGVV
                     LAVNPCFVRLITHIGAAPNCALGSCCGVPDGGYGSYGNVRTVGSVTDIPLDRIAAVVR
                     NAI"
     gene            1078..1410
                     /locus_tag="PKI50_00015"
     CDS             1078..1410
                     /locus_tag="PKI50_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02135.1"
                     /translation="MANGIGYGGNFNGNFFDLLSDLIGETVTIFTTSGGQSGCGFTGV
                     ILAVNQCFVRLVTHIGPAPGCALGNACHSPCCPTIGCSGGFSNVRSVGSVTDIPLDRI
                     VAVVSNAV"
     gene            complement(1469..1828)
                     /locus_tag="PKI50_00020"
     CDS             complement(1469..1828)
                     /locus_tag="PKI50_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNT02136.1"
                     /translation="MFGSGKKSINSERVDTIIGRGTIFEGTVNADGTVRLDGKVLGGL
                     NISGNLIVGEEGAVKGNVKTENAFVAGVIEGNVIATSQLHITHTAKLVGDITVKNIII
                     DEGAIFIGNCKIITDNT"
     gene            complement(1866..2813)
                     /locus_tag="PKI50_00025"
     CDS             complement(1866..2813)
                     /locus_tag="PKI50_00025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT02137.1"
                     /translation="MKKALVKNKINYTTFMFIPESQSNVKSFRTPAWFPGFVSISIAL
                     LIITSGISFYALSALKLQYDASRKEISKLTTINASQKIEIGNLQRSSEEVQKQLAENS
                     RMLDEVKKAVGINPSPEEGIEIKLVSPEISSPVSLLTEKSDQDMSLKLASIEADFASL
                     TDKIAIQKKTIADSIDPIKKQLAYLMAKPSVKPVAASITASFGYRKNPFTSRGSEFHK
                     GIDFAASYGQTVSATGDGIVSFAGWNAGYGRMVIISHGYGLTTLYAHNSKLLVKQGDK
                     VKKGQAIAKVGNTGRSTGTHLHYEVKLNGKNVNPANYFN"
     gene            3105..3374
                     /locus_tag="PKI50_00030"
     CDS             3105..3374
                     /locus_tag="PKI50_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HNT02138.1"
                     /translation="MNKVGIEKPLSNVGRYLESRDCAVEVLEDDTKESPGNLNKFDAI
                     VVTGADSNFMGMEDVMTTAKVINAEGKTEDEIYSEILRAADLKKR"
     gene            3495..4271
                     /locus_tag="PKI50_00035"
     CDS             3495..4271
                     /locus_tag="PKI50_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNT02139.1"
                     /translation="MIHVGTSGYSYKDWIGTFYPAGAKAGDLLGIYSRSFDFTEINST
                     YYKIPNSFMFYNMQKKTGDNFIFTVKLHQSMTHGRDAGDKDYEEFAGAVDVLKQAGKL
                     GCLVAQFPYSFHFNRENMDYIQGLRERFADFDLMVEFRNNRWVNDETMEFLRANQLGY
                     ICVDEPAVSGLLDPRAMRTSEISYIRFHGRNDAKWWDHKEAYERYDYLYKERELEQWK
                     DKILELDKQSQNCFVSFNNHFRAQAVINGIMLKKLLGMME"
     gene            4298..4849
                     /gene="thpR"
                     /locus_tag="PKI50_00040"
     CDS             4298..4849
                     /gene="thpR"
                     /locus_tag="PKI50_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNT02140.1"
                     /translation="MRTFIGADFSRDIKDSIAGVQHIVRENSERGRFKYVGNFHLTLK
                     FLGEIEQAKAPDIGRTLKEIALKHRRIRLNIDKIGYFDGKGSIHALWLGFSGELDKLS
                     SLYSDIDEGMYKLGIKKEIKAYTPHITIAQDLVLKIPFEKLEEKLNFQNFRTVEIREI
                     SLIKSEEAGGKRIYTPISTFQLK"
     gene            5072..6922
                     /locus_tag="PKI50_00045"
     CDS             5072..6922
                     /locus_tag="PKI50_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNT02141.1"
                     /translation="MISRDDFLPISKEDMQRKKIEQLDFIFVSGDAYVDHPSFGSAVI
                     TRLLESRGYKVGIIPQPDWRNADAFKALGRPRLAFLVSSGNMDSMVNHYTAGKKPRRE
                     DLYSPAGVRGKRPDRAVTVYCNRIREAFKDVPVIIGGIEASLRRFAHYDFWDDQVRRS
                     ILFDSRADLLIYGMGEKPVIQVAEALESGMEIKYITYINGTAYIAPELGNIPDATVIP
                     SFDEVRDSKRKYARAFMLQYREQDYHTGRVLIQPHGERFLVQNPPAAPLTETELDDVY
                     SLPFTRQYHPVYKNQGGIPALKEVKFSLTSQRGCFGGCSFCALNFHQGRIVQSRSHES
                     IIEEAELLLKDPEFKGYIHDVGGPTANFRHAACDKQLNAGVCKNRQCLYPTPCKNLKV
                     DHKDYVALLKKLRSLPGVKKVFVRSGLRYDYIIADKKDEFLRELCENHISGQLKVAPE
                     HVSDKVLRYMGKPSKEVYGRFVKKYFDMNKRLKKEQYLVPYLMSGHPGSDLSAAVELA
                     EYVRDMGYNPEQIQEFYPTPGTLSACMYYTGLDPRTMKPVYVPRDPEEKAMQRALIQY
                     RNPENYGLVYKALKSAGREDLIGFGEKCLIKPPRNPEKRRNPRRENRKRR"
     gene            6947..7450
                     /locus_tag="PKI50_00050"
     CDS             6947..7450
                     /locus_tag="PKI50_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT02142.1"
                     /translation="MKAILERRSIRKYTGEPVSDADLKDLLKAAMAAPSAGNGQPWEF
                     IIVNDRQLLTEITKVHPYSAMLLQAPLAIIVCGKLDNERYKGYWAQDCSAATENILIA
                     AQSKGLGTVWLGVYPVEERVNGIRKIFKIPENMIPLNVIAVGHPAEKKKPSDRYDENR
                     IHFNKWE"
     gene            7532..9058
                     /gene="hutH"
                     /locus_tag="PKI50_00055"
     CDS             7532..9058
                     /gene="hutH"
                     /locus_tag="PKI50_00055"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNT02143.1"
                     /translation="MGHLSIDGEHLTIKDVVDVARNSRRVGLSEQAVERIRKSRDYVD
                     SLVDKEAVVYGITTGFGKFSDVFISKEDTRALQRNLIVSHACSLGDPLTEETVRAIML
                     LRANALAKGFSGIRLETVNTLVEMLNKGVHPVIPEKGSLGASGDLAPLANMVLVMLGE
                     GEAYYKGVRMTGKEAMEKAGIKTVELTSKEGLALINGTPVMTGIGALAVYDAENLLKN
                     ADIIAAMTLEALKGITTAFDRKVHEVRGQKGQMTAARNMLRIVSGSELTTTQGQIRVQ
                     DAYTLRCIPQIHGASRDAVQYVKEKVSIELNAATDNPLVFADDDQVISGGNFHGQPIA
                     LAMDFLGIAVSELANVSERRLERLVNPQLNDLPAFLTRKGGLNSGFMITQYAAASIVS
                     ENKILAHPASVDSIPSSANQEDHVSMGTTAARKARVILDNVQKVLGIEAFAASQAISF
                     RGDVRLGKGTSEAYKLIRKEIAPVEEDRIMYTDMNKLDEMVKSEALIRAVESAVGELE
                     "
     gene            9064..10302
                     /gene="hutI"
                     /locus_tag="PKI50_00060"
     CDS             9064..10302
                     /gene="hutI"
                     /locus_tag="PKI50_00060"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNT02144.1"
                     /translation="MKADLIINASTIVCCTEGEGAGVFENGYVAVKDGKIISLGEGNA
                     PGDIKGSGTRIIDAAGKTVTPGLIDAHTHLVHGGSREKELPLKLKGVPYLEILKMGGG
                     ILSTVRNTRKASKEELKAKSGKSLDQMLLHGTTTVEAKSGYGLDFDTEIKCLEVAAEL
                     NDSHPVDIVSTYMGAHAVPEEFKGNTQGYIDFMADKVMPYVRENNLAEFMDVFCEEGV
                     FSPGESRAIMQKGKEQGFKLKIHADEIVPLQGAELAGELGAVSAEHLLAATEAGIAAM
                     AEAGVTAVLLPGTSFYLMLGKYAQARKMMDRGVRVALATDYNPGTCPTENLQSIMTFA
                     CFGMKMTPEEIIKGMTLNAAYALDRAGETGSLEVGKKADIVVFDAPNLEYIVYHFGIN
                     HVDTVIKDGKTVVENGRILK"
     gene            10319..10963
                     /locus_tag="PKI50_00065"
     CDS             10319..10963
                     /locus_tag="PKI50_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNT02145.1"
                     /translation="MSLLMDMTLRDYSDVLASSEPAPGGGSTAALSGVLGAALTMMVV
                     NLSVGKKNYENLNEKIKAKFMEEAENVKALQKELSGLVDEDTKAFNKFMEAVKLPKDT
                     EEQNQLRGKKMQEASLYALQVPIKTAECCFRLLENQGNIARYGNRNAVSDVGVGALLT
                     LSGLEGAILNVNINLSGIEDEAVRSEAYDKCRRLSAEGRKLHSEILDIVNLRIG"
     gene            10971..11201
                     /locus_tag="PKI50_00070"
     CDS             10971..11201
                     /locus_tag="PKI50_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HNT02146.1"
                     /translation="MISREMIDRINHLSKKSKEAGLTEEERQEQQELRRKYIEYIKGQ
                     VRHGLDSIKFAEDRHDCGHEHHDHCGCKCKKQ"
     gene            11253..11723
                     /locus_tag="PKI50_00075"
     CDS             11253..11723
                     /locus_tag="PKI50_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT02147.1"
                     /translation="MAELQFVVFKLGKEEYGVNIMQVQEIGHYSEPVKVPNTPEFVEG
                     IMNLRDNVIPVISLRKRFNIPEENLNGPERDLNENRRTIIINLGDRQIAFIVDDASEV
                     LTLDEAGIQETPDIIAGVDRKYISGIGKKGNRLIIMLDLNFLFDEQEQAQLAAI"
     gene            11879..13021
                     /locus_tag="PKI50_00080"
     CDS             11879..13021
                     /locus_tag="PKI50_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNT02148.1"
                     /translation="MDRLNEIIKNTVDIIEKSRSELFSIAESSRTECQLVEEELEKIR
                     IIASDLINQVDMLVKLDKECRYQLMIVSKNIKDYKEEDVKRAYDKAKDLQVELMTKKA
                     EEQLLIQRRSELERRLKIARDTANKAESLVNKVGVAMGYLTGELQDLSIQMEDIKQKQ
                     YLGIKIIKAQEEERKRVAREIHDGPAQLMANLVIKTELCEKLIDMDKEKAKEELGSLR
                     GFLRSSLSDVRKIIYDLRPMSLDDLGLVPTLQRYISNYIEQNNISVNFSVLGDTKTLK
                     PVVELAAFRIVQEALTNIKRHSGADNAVVRVEFTNDQVNLLISDDGKGFDKNKLWKAK
                     EEDAGYGLLNMKERVELLNGKFDVKTARNKGTKVFASLSLNIAEEG"
     gene            13023..13667
                     /locus_tag="PKI50_00085"
     CDS             13023..13667
                     /locus_tag="PKI50_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT02149.1"
                     /translation="MSNIRILIADDHSIVREGLKQLLELEEDFEVVGQASNGVETIEK
                     VEELKPDVLLLDINMPIMNGIKALKKLKEDGIDTRVVILTIHEDREYLLETMQIGASG
                     YILKDSDSASFFKAIRDAYIGESYIQPKLAADLIKEFNKPRGARVKNENELTQREYEV
                     IVLIADGLNNKDIADRLFISEKTVKNHVSNIFRKINVSDRTQAAIYAYKNNIKK"
     gene            14031..14294
                     /locus_tag="PKI50_00090"
     CDS             14031..14294
                     /locus_tag="PKI50_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02150.1"
                     /translation="MTIARVCGSIENTTVRKFDRGGKSMNMIIKLLTGLKCKDRENRD
                     LNDCGYTLYQSLEYNLKYLKGDDFGYRDPRTQSTDDLMARKTF"
     gene            14466..15623
                     /locus_tag="PKI50_00095"
     CDS             14466..15623
                     /locus_tag="PKI50_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNT02151.1"
                     /translation="MDKQKLYTLLQKPEGTKLDFKAVLSLKTESEKKELAKDVAAIAN
                     SKGGRGYIIYGIQDETKAILGIEGKRYTEEQIQQIISQRCDPPVSVKLEIIPVDDKQI
                     AVLTIYRSSQKPHQIRQTGVFYIRRGSTTDIARREEIASMLQESGILQYERIVLNRVE
                     LKELNEDIISDYISKTGFTSSSENYYTLLEGIGIIGRDEDSNGYHPTVGGLLVFGYNP
                     QLHLPHAGIRLIDKSAADKVIYFSGPIIRMLDEIEDYLRFKAEKINGKYPVAAVVEAV
                     ANAAVHRDYFSPGREIVISIDNKKIEISNPGANCSDDDIQGLMEEYNPCRRNQWLYQR
                     LLILDNKGRFLNIGAGMKKIHEAFKQHGGARFINNEKRNLFKVVLPGFMKY"
     gene            15657..17348
                     /locus_tag="PKI50_00100"
     CDS             15657..17348
                     /locus_tag="PKI50_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02152.1"
                     /translation="MNNKVVTFERGADFYFDLSYKYIQKGRLKTALRYIEKAVSLKPH
                     DSFLQFNYAGLLAEQGHIDRSTEVLLNIVKNIDPNYLECYFGLGCNYLQLQKIKKSSE
                     YFSIYLEKDPGGEFAEEAEELLEMLTMIKDANNNMDDEELEKIYKIEEEAIDHLEKKE
                     YKQATEKFEIVVQMLPNAVPARNNLSLAYYYLGFIDKAIELALEVRTYEEANVHANCN
                     LAIFYNKKGAASLVKKQLGIISRIRADSADYLYKIGDTYGSLGKHADAYKTFKKLLII
                     DSKNPIYVHLAAVAAFNSRKYRESIRLWEKLGQIDKENMLSDYYIEAAKNQLDAEDER
                     TYFPYLYQLPKEEIKNRLDFIYKFIEMPGPKCREQLADNEHMLGMLYFGINFDKYFLR
                     KLIFNKIKNDRMNEAESFIRRFMLRDDVENYIKMEAVFLLNFIGAKEPYTVNFDGEKR
                     NVTIDSLDFPESEWEKPWIKVKRKAISMMRNKYKRPYKKIVEDIWYEFIKSTFPEVPE
                     IDDVNIWAAALEHTYCRLCNAEASEHKLAENYNISSVSLLEKAEIINEAIKNKFQ"
     gene            complement(17401..17967)
                     /locus_tag="PKI50_00105"
     CDS             complement(17401..17967)
                     /locus_tag="PKI50_00105"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT02153.1"
                     /translation="MAKQMWKPGTMLYPVPAVMVSCRSREGVSNIITVAWTGIVCSEP
                     AMLYVSIRPERFSHGIIKDTGSFVVNLPTESLAYALDFCGVKSGRDINKFEHLGLTQQ
                     KSNLVDAPSIGECPVSLECRVNNIISLGSHDMFIAEILCVDAEEKLLDKSGKLHLNKA
                     GLICYNHGEYRALAKSLGHFGFSVRKKK"
     gene            18269..18880
                     /locus_tag="PKI50_00110"
     CDS             18269..18880
                     /locus_tag="PKI50_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02154.1"
                     /translation="MAHGKDNNVSDSRMNELKNMVSEKLGGMDIDNVSPDQVKNMLVN
                     EISRNSNIDPAIKDKINKGDVDGLKEDIIKYLSNNRSADGSSDQLINMLKDNDMEGLK
                     KQLMGMLLSGLGPQKKNEIKEPEVYESQADLKDSLGDFDERALLGLLFDKMFAGVKDD
                     SRISLLYSLRPFVSEKRQRGIDDCIKIMNLVAFFENFMSKAGK"
     gene            19251..20582
                     /locus_tag="PKI50_00115"
     CDS             19251..20582
                     /locus_tag="PKI50_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNT02155.1"
                     /translation="MDGDLWTQILLIILLIGLNAFFAASEMAIISVRQSRIKPLVDEG
                     NKAAILVNRFLEEPSKLLATIQIGITLAGFLASAIGAQTLTNGLTGFLMSLNIPFLSA
                     SAVGISTFIVTSIIALFTLILGELVPKRMALSQSEKTAFRVAGTINLLSKLTYPIVKF
                     LTVSTNLVVKLMGGPAKVTEEQITEEEIRSMINVGEEKGIFREAETEMINSIFEFDDT
                     VAKEVMTPRIDIVALNSEATREEILDVIVQENFSRIPVYEESIDNIIGILYVKDLFAM
                     IRNNVEWEVSLKEIIREAHFVPEYKKIDELFKEMQKSKTHIAVVIDEYGGTAGIITIE
                     DLLEEIVGNIFDEYDDVILDYEKIDDNTYIVSGMLNVSDVNDILNTKLSGEEEFDTIS
                     GMLLSHSGKMPEVGYELTIDNIYFRIEEVDDKRISKIRIEKRDIEPDKTEE"
     gene            20770..22209
                     /locus_tag="PKI50_00120"
     CDS             20770..22209
                     /locus_tag="PKI50_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT02156.1"
                     /translation="MRFTDSRSKILLSERWLSVIAHSLFSSWMLSFIFEGEVFYSLMG
                     IFDFDSHAMVFGGIAAVFLGLLLSGFFIKTKRGAKRTFLYSYLFCIVMSVPFFFPPSV
                     LWTVGIIAGSFMSGSCVAAWGYYLKSSTPKIGRIKTVADMLILSNILMILLNMAALWI
                     SPHSGLALSMIMLILAFLFALKLPGDNCISSSASFERDKSRISIAPLLVFLCIFIIVI
                     TINSGLMYQVVNPAFAHLKWLTSWYWAIPYIIAVFIMGHLPQKVNRAYILYVAIAMIG
                     ISFISFMILDHNVAGYIAVNTLMLGAFGVCDLFWWSILGEMLDFHKNPAAILGIGLSA
                     NVLGILLGGLIGNAIVSVGKQSADPVLLALAVVCVALALLPFLHRYLSSLLRDHTYLR
                     IFSEISAQEQIRQPDYDAVFESLSERENQVASRLLQGKTYRIIAGELYISENTVKYYV
                     KNIYSKLKIQSRGELVNIFLEKRGIGTGK"
     gene            22364..23749
                     /locus_tag="PKI50_00125"
     CDS             22364..23749
                     /locus_tag="PKI50_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNT02157.1"
                     /translation="MKKANRKKITWGLVTTALLLTISLSVNFLIPETVYADTTGGWHL
                     AETNYYLSTSDYTVKGEIQNGIIGTSDVIYDKYMVEGSEGDMTFTHSRRYENGNAIAH
                     VTYQVVWDTPPSYIPAGQKTGFNIDYKVISSKSWGGVQINAHLDAAGIKPGGATSSYI
                     SFQLEDGTKYINSDKQGYFESEKVIPEGRPGDKKAIILTLGNGYGYTYIYEWKEGSAP
                     VAIPTAPAAPVSSGSGAEPFESGARIMWQPANGLGYRLFRSDAPNDIGISVTDFYITS
                     TSYADVNVEPKTTYYYSVKPVLAEARPFEGIDEKLGDVIATFVVTTGDQVYKPGSYKH
                     FIMLKLEDPNMSVDGVSQEVDPGRGTAPVVIAGRTMVPIRAVVEAIGGTVEWDGATKK
                     ITLTARGNTVEMWLGKTDIKINGESKKMDIAPVSKNDRTFVPVRFAAENLNCKVDWIN
                     STKEAVIVYEE"
     gene            complement(23868..23953)
                     /locus_tag="PKI50_00130"
     tRNA            complement(23868..23953)
                     /locus_tag="PKI50_00130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23917..23919),aa:Leu,seq:caa)
     gene            24143..25084
                     /locus_tag="PKI50_00135"
     CDS             24143..25084
                     /locus_tag="PKI50_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNT02158.1"
                     /translation="MDPVTHAAVGLTIAVMSGEPLAASNPAMIACVAGAIIPDGDILM
                     QLKGDYAYLKNHRGMSHSIPMMFVYSGIITGLLMLAFNGAAFWNIFPLALLGCFSHIA
                     LDITNSYGAQILWPLSKKHITFDLLLVYDPILIILCLINILPYTRGIIPPYMTVFIFA
                     GYLILRRIMKKLAQDIAYQNLGYKYKSEYFRIMPSMIGIIKWHFVLYTGRRKIIGEVN
                     IFPRRFRIIDNKKNIDTDLYKIAMDTPVGMFFEKFTPVFHVECEKKEGNYEFEFIDLR
                     YHISKDFLHHATAVINRDLEVVTSLFHPYRKTRNVEV"
     gene            25298..26680
                     /locus_tag="PKI50_00140"
     CDS             25298..26680
                     /locus_tag="PKI50_00140"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNT02159.1"
                     /translation="MKRSFFIALIVIICFSRVLADSGSDKSSIEINLPSGTLSLYKDG
                     NLAKEYPLCLGKQSTPTPAGQYRIIYKAVNPYWLNKGDVVPPGPQNPLGVRWMGITRG
                     IGIHGNNKPESIGTYASAGCIRMFNRDVVEVYNQVTVNTPVNIKYDRLKLFEDKYSMK
                     KSIIIYPDIYKKGAEIAGQQLRQLSNTDISEEILKKAQEIIAKPIAKPLAVSDGIGVF
                     LNNSLMTCDALEEEGKIYVNYKAAEDILGLTPELAGKFNIGIKELEGVIYINLNDTAD
                     RFGGTVSYDEAADSAYIDMEVVKVNGVFAGINYGDYDKSDYLTVETVKHLGYEYSEDS
                     VDIRVFDKGIMKLKRKNAWVINLDNLAEVVGGSKDTSSRYGIVDLRLPAYLKVGKEYF
                     KTDNIEGKPVLNAETAYSIYERSGRAVETFSAQGQNIDEKIDLETFLEGHDYTVNDFY
                     TLIDIKLKEN"
     gene            26936..27529
                     /locus_tag="PKI50_00145"
     CDS             26936..27529
                     /locus_tag="PKI50_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT02160.1"
                     /translation="MSDLEKLLIKKSQSGDVESFELLISSYDKRAYNIAYRIMGNEED
                     AKDMAQEALLRVFKSLKDFKGQAAFSTWLYRIVTNVCLDELRRRKNEKYVSMDSTIHT
                     EDGELHMELCSDKETPESVYERIEQRELIRNAIREMNEDYRTVIVLRDIQGFSYDEIS
                     GMLDCSLGTVKSRINRARTMLRDKLKESMELSRENIV"
     gene            27555..28904
                     /locus_tag="PKI50_00150"
     CDS             27555..28904
                     /locus_tag="PKI50_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNT02161.1"
                     /translation="MKCETIKNMMSSYIDKDLNNIEKAEFEKHLAECEECRAEYESLI
                     DIIAACGNLEEIELPQNFRTELHQRLNEEKSKRNFFSGIMGSKRMKMAAGLVAAALVI
                     AIGIGGSSLLFDNSMKMAQSADSAPGYGIAPAPAAMPEAPAAPPVYYDSDLAENKAKS
                     REFETAAAEERGMGEDSVGMQFNESLMAKEMAPSQGLEGQAAESARSGRMVIRSGNIS
                     VNVENVDKAAADIRQMTESSGGYVENSQIDNVTVPQFPVYDAGSEARETTQKYANMTV
                     RVPENRFEDIFNNIKGMGKLVSENISGSDITAEYRDTTARVDNLKIQEQSLQQLMAKA
                     KTVDEILKIESELNRVRTDIDINSGNLKRWDNLVQLSTIYVYMRELKPEELKSVDVPG
                     TWEKAYQGFIKAVNNVVEGFQKTLIVLVAAIPYLFVAGVIAAIGFLAARKIKLKKKQ"
     gene            29044..31653
                     /gene="polA"
                     /locus_tag="PKI50_00155"
     CDS             29044..31653
                     /gene="polA"
                     /locus_tag="PKI50_00155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNT02162.1"
                     /translation="MEKGKFIIIDGNSLMHRAFYALPNLTNSKGFHTSVIYGFVNMIN
                     KIKEEYKPQYIGIAFDTKAPTFRHQEYAEYKAGRLKMAEEMAEQIPVLKEVLAAMNIR
                     QVEIDGFEADDIIGTVAARCDGEGIPTFIVTGDKDAFQLISDNVHTLMTKKGISEIEE
                     YDTAKLHEKYGIAPAQITDLKGLMGDASDNIPGVPGVGEKTALELIHQFGTVENTLEN
                     ASEIKKNKVRENVSSNMDQAILSKRLATIVKDVPIDFELGDFALKEADKEKLYSLFAE
                     LEFKGLIEKMCGSNSCTEAAGTVEIAVKEIDGIDELKKVFEEVSKCGKFAFKIDDELG
                     EMLRTIYINADGKQYRFNINDSNMPLVKEIMEDSGVLKTGHDIKQDILLLRRYGIDVG
                     PIGFDTMIAAYLLNPSKGSYRIKDLSQEYLGYGVDGYDAKGAEQVQKYAATVKAAAEL
                     QPVLTGRLEEYGMIELYNSVELPLVKVLADMEHEGFSVDKAMLEVLSGDFAKQIDALT
                     SEVYRLSGEEFNINSTRQLGTVLFDKLGLPAMKKTKTGYSTDVEVLEQLYDKHPIIQK
                     LLEYRQLVKLKSTYVDGLIGIINPETGRIHTKLNQTVTVTGRLSSTEPNLQNIPIRTE
                     NGRQIRKVFVPRSQDYILVDADYSQIELRVLAHISGDKGLIDTFKRNEDIHTRTASEV
                     FGIEIKDVTPLMRGRAKAVNFGIVYGMSDYGLSQDLKISRKEAKKYIDNYFARYPGVK
                     QYMTDIVEAARTDGYVKTIMNRRRYIPEITSRNAVNRNFGERIALNTPIQGSAADLIK
                     MAMVKVYDELKKRNLKSRLILQVHDELILETHKDELSEVKNLVKECMENAIKLSVPVI
                     ADISSGDSWYNAK"
     gene            31672..32268
                     /gene="coaE"
                     /locus_tag="PKI50_00160"
     CDS             31672..32268
                     /gene="coaE"
                     /locus_tag="PKI50_00160"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907570.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNT02163.1"
                     /translation="MVIGLTGGIASGKSTVSAKLKELGAAVIDADLLARDVVRKGEIA
                     YNKIVQCFGADILLPGGDIDRKKLGDIVFSDKEKLELLNSITHPEIINRMKERIQELK
                     AEGAKVIVVDAAILIEMGLHKYVDSVWVLSVDRETQLKRLAERDKYDYREAENRINSQ
                     FTDEVRKKYADVVIDNSKPIEEVEKRLEELWNNIAGGE"
     gene            32272..32829
                     /locus_tag="PKI50_00165"
     CDS             32272..32829
                     /locus_tag="PKI50_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT02164.1"
                     /translation="MAKVRKLKRIRLLVAVLLIYAAVLNIPNLLKLLYPIEHKEIIIK
                     YGQMHNVDPLLLAALIKTESNFEPRAESRKGAKGLMQITPSTGEWIAKTIGVNDFNED
                     MLFDPETNIMLGSWYIEHLTNYYNGIFELVFAAYNGGRGNVDKWLKDKNLSSDGITLD
                     TIPFSETKNYLEKLRKNYNIYKIIY"
     gene            32885..34483
                     /locus_tag="PKI50_00170"
     CDS             32885..34483
                     /locus_tag="PKI50_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT02165.1"
                     /translation="MKDKRFLLIFILLMLLSLVSCEESVVPKETEIAVENEEPVEGGT
                     INISSVEPGSLNPLLNKSKSYNEISKLIFQSLVEYDANLKIIPVLAQSWDFMDGSNKF
                     VIKLKENVFWSDGESFTADDVKFSLDTIKASEDSIYKGNLEHIYSYKVKDASTIEITF
                     DQPFVTAIDMLSFPIIPRHIYKSNPNAVPIGTGMFKVSEYNRLKSMELVYNDKWNGEE
                     KPYIEKIRITFINDVDAFSIAFQSKELDLLNTTSYDWEKYSEMKDINAYKYTTMYYDF
                     IGLNYNNPIFQDKAVRKAMIQGINRKSIVDKYLLGNAAVTDVPINPTSWLKDADAEKY
                     GYSRSEAQNLLMAAGFADLNNDGILERKVAEAVQQLSFTLLTNDENEFRRKAAEEIKK
                     NLEDIGFRVEIKFAGFDDVKAAIETGEFDAVHTGYNLSNTQDLSFAFHSTQIDSGKNF
                     MAYSNPEIDALLLQAYTAMDDNRRKEIYKNLQKSFREEVPCISLFFREAALVVRDKIQ
                     GEIKPDTVNPYRSIHKWFVPESKR"
     gene            34547..34629
                     /locus_tag="PKI50_00175"
     tRNA            34547..34629
                     /locus_tag="PKI50_00175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34581..34583,aa:Leu,seq:gag)
     gene            34892..36211
                     /locus_tag="PKI50_00180"
     CDS             34892..36211
                     /locus_tag="PKI50_00180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT02166.1"
                     /translation="MIKHTYLSVFALSAWVGTAMIRSAKRYGKLALFLGLLNIAWVVN
                     TVIFSYILEIPQIAPYIVSQIILLLNAIGLIQFFLKEQRNEIERRSDQIAYLTFHDEL
                     TGLHNRAYFEKKIQELSKDNDCLPVSLLIGDMNGLKFINDVFGHQKGDKWLKRIAAII
                     QQSCRQNDIIARWGGDEFAVIMPNTDREKALDIAHEINEACKRNQEEDSFFSISLGVA
                     TKNDSGTDLSKVLTEAEELMYQNKLIEGKKTRLAFVEVLGNLLHKKGYEPKEHIERLA
                     ALAKEFAAVLNLSRENRKYLVQTANLHDIGKIGIPEDIVLKESRLDESEWPIMKKHVE
                     IGYRLAQISGEFAHLADVILYHHEWWNGQGYPHGLKGEDIPLLSRIISILHAFDVMTH
                     QQPYKPAGTIDEALHELSLNAGTQFDPALVPVFIDMISDIALDKDCN"
     gene            complement(36213..36392)
                     /locus_tag="PKI50_00185"
     CDS             complement(36213..36392)
                     /locus_tag="PKI50_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNT02167.1"
                     /translation="MDNKEKEKKQIPLRLSSSLWKELAEWAEDDFRSINGQIEFLLTE
                     CVKQRKKRNISKPQE"
     gene            complement(36433..37473)
                     /locus_tag="PKI50_00190"
     CDS             complement(36433..37473)
                     /locus_tag="PKI50_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNT02168.1"
                     /translation="MSEKSNVAENQLHYQEVLPKTINGMLMLVVNIILYIASIALFIF
                     AVNLIDSGKNIILGVILIIVCVIYAFVAGPLLFIGLKILKPNEALVLTLFGKYYGTLK
                     GAGFFFVNPFAAAANNATHAAATAVLSADISPLAGKTAGKAADAVFRNKKIISLKAMT
                     LSNDKQKINDQLGNPIIIGIVVIWKVVNTAKAVFNVDNYIEYLSIQCDSALRNIVRLY
                     PYDASSEDNEKSLRGSSQEIADKIREEIQRKVEIAGLEIIEARITHLAYAPEIAAAML
                     QRQQASAIIDARQMIVEGAVGMVEMALAKLSENDIVQLDEERKAAMVSNLLVVLCGNK
                     DAQPIVNSGSLY"
     gene            complement(37580..37762)
                     /locus_tag="PKI50_00195"
     CDS             complement(37580..37762)
                     /locus_tag="PKI50_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02169.1"
                     /translation="MRDRRKKPIKKDFDYEEDYYDRLKHGSKSNNPYRGFTNKATGKK
                     TEFDYEEDAYEPEKNK"
     gene            37893..39410
                     /locus_tag="PKI50_00200"
     CDS             37893..39410
                     /locus_tag="PKI50_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT02170.1"
                     /translation="MKRAIGIMIMVFIFAAVLTLYGLAEEVPYKGASPWAVPELDKAA
                     GYGLITDRIKDNMSVPITREEFAEIAVRLYEINTGKAAEYSDTDVFSDTDNPEILKAY
                     SLKIVNGIDLQRRLFLPKTFINREQVAAMMFRTIQAAPLNFEFPAGKGGIFVDENEVS
                     DWALESVKFMTENGFLRGADGRINPKGTCTREMAVLIAARIYETYLSAAEAPAEEPAE
                     EPAEEPAEDEGTTGSEPGTGSYDWDQIVINDTVIFRDNYYIRNKDGFYYIFIGAERLK
                     YAFKLRNAGSYTYPEVEVIGGSITAEWSSGENVVLKAEMREGSTEAIVGGKIVDIGMA
                     PYIIAGKMYIPINLFISALEMDVETSSNDNILFIQYKDAFPAEILSGIWSDTGIDLFA
                     AAKDLESGSGNLPSYATAYRFNSDGTYELIKVSAAGINDTIIKQRGKYKVMGNTIMYY
                     DIFETIYKGSPFSLKYRDEPVDRPHYEFVYNYDSKEEIIEIGGFWLNRVQQFPRK"
     regulatory      39477..39670
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            39780..40688
                     /locus_tag="PKI50_00205"
     CDS             39780..40688
                     /locus_tag="PKI50_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT02171.1"
                     /translation="MKKIIMLLIACITILSLFGCVDTQQKKEFEDAGKIEVTDIKGRS
                     IVLDKIPQRIVSLSPSSTEILFALGAGDRVVGVTNYCDYPEEARKAEKIGDYDEPNME
                     LIRKVQPDVVLAGHVKEEVAEALEKMGIPVIITEAEDFDTIYHSIKLVGQVTGTTVQA
                     EAIVNGMKSKIEEIEAKFKDKEKPTVFYLVWKDPLFTAGSETFINDVIKAAGGINVAE
                     KVEGWANYSAEEMIKDNPDMLIAALHSTDEGMTREDLSGDRLFSLLECVKQGKVYIMP
                     DDNIISRPGPRIVQAIEDMSKVLHGE"
     gene            40841..42052
                     /locus_tag="PKI50_00210"
     CDS             40841..42052
                     /locus_tag="PKI50_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02172.1"
                     /translation="MEMNIGKGSFFCIVGPNGSGKSTLLRVISAALKPQEGAVYLDGR
                     SINGIKHRSIAGMLSFVPQNTSLEFDFKVSDVVLMGRYPYINKLKGETGKDFEIAEKA
                     MEYTNTTHLKDRSFMELSGGERQRVILAQAFAQDTDILILDEPVSHLDLQHQVEILNL
                     IKKMCVDRKLTAITVLHDLNLASAYGDYIVMMKAGAIMKQGTPFETLTADGIKEIFDT
                     DVYVSVSPVGNKPYIYALTKPNIEKKGIRVHVICGGGSGSDIISRLCSEGFDLSSGVL
                     AIGDLDWKISKENDMQVAEEVPFVGISGEAYAKNKELAMAADAIVLTGLYLGKSNLKN
                     LELLLEKDLEGKPLFILEDESFAARDYTGGAACRLYEMIKFRNNSILTDSKLLKEEII
                     KAVETHGESKE"
     gene            42033..43037
                     /locus_tag="PKI50_00215"
     CDS             42033..43037
                     /locus_tag="PKI50_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT02173.1"
                     /translation="MGKVKNRNWQVFVFLIASLILTLIFATSTGAVKISTAEIIEILA
                     KKDSDIVNASILMDIRLPRVLLAVVLGAGLAAVGGVMQAIFKNPLVDSYTLGMSSGAA
                     LGAVISIITGLNARILGMETTGVFAFIGAVLTLFFVYSLAYTRNRMSINSFLLAGVAV
                     SYFLASVISFLMMLNHDKIEHIVFWTMGSLSMATWNEFFISAALIIPSLAVLMYYTRE
                     LNILTMGEDAAHHLGINTFALKNVLLITCALVVGSVVSTGGTIAFLGLVAPHIIRLII
                     GSDNKRVIPYSALLGSILLVLADTVGRVLIQPAEIPVGVMTSIMGGPFFIFLLRKSKN
                     "
     gene            complement(43198..44223)
                     /gene="ltaE"
                     /locus_tag="PKI50_00220"
     CDS             complement(43198..44223)
                     /gene="ltaE"
                     /locus_tag="PKI50_00220"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNT02174.1"
                     /translation="MRYIDFRSDTVTQPTNEMREAMAKAIVGDDVYGDDPTVNLLEEK
                     AAEYAGKEAALFVPSGTMGNQLAVMTHTKRGNEIIAEENCHIIQHEVGAVAILSGAMV
                     RTIRGEKGVMNPQDVLKAIRGNDIHYPETGLICMENALSNGTVVPLEVMKEIYTIAKD
                     HNIPVHLDGARLFNAAAYLEVNAAQITQYTDSVMFCLSKGLCAPVGSMLAGSKEFIEK
                     ARKNRKILGGGMRQAGILAAAGLIALEKMSKRIHIDHENAKYLAERLMSIPGIKLNYD
                     DVHIDMVFFDMEETGISSDKLEDQLFKKGIKINGAEGGLMRFVTNNDITREDIDYTIQ
                     CMKKIIK"
     gene            44494..45093
                     /locus_tag="PKI50_00225"
     CDS             44494..45093
                     /locus_tag="PKI50_00225"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNT02175.1"
                     /translation="MSKNKLKLICVFSLVLIIALYSVLFYAGRLPAAYTAFTENIGMS
                     KVSRQLFEAYNVMDNTFHFQLPDSWSAYEASFAGEEILYHLNFISKDKKIHGFVQVWK
                     LSEPLKQFIEKSKESAVGVIDFKNFDIKEITVDNKNGYLMDYSRANQKGEYIRAYEAF
                     IKGFSNKVYRLSFFVPEKEWKEYYRILFDRIIHLVKISK"
     gene            45252..46028
                     /locus_tag="PKI50_00230"
     CDS             45252..46028
                     /locus_tag="PKI50_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNT02176.1"
                     /translation="MPINLVLCEDKHIILNSIKASLEKAAKQNGIDANICLMTDNPKR
                     IIEYSRQYSQGVNAYFLDINLETDMNGLELAKQIRSHDPHSYITFITGHLELCMTVFK
                     YHIEAFEYLVKPVSYQAIEECLISIDKQYSQYLSNQKYNKDAMIIIRSGICDYRVELY
                     DIIYVESINQKLVVHTRSRNIEFFGYLKDIIKDLNKNGESFYRCHRSYIININHVKEV
                     NYRDSSIIMSNGEKCYMSRQQKSEMRNILGRMGQDYDDII"
     gene            46012..47307
                     /locus_tag="PKI50_00235"
     CDS             46012..47307
                     /locus_tag="PKI50_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HNT02177.1"
                     /translation="MMILYKIFINTIGMVLFAYLAQALYNFQREKKVFIKFIIYLAIC
                     TNILFYLDKYIGAGVRAIAVLLMMMLMSYFVLKLNVLQSIVVNITNGIALVIGDISAV
                     FIMVKIYGYTHEMIKSNIMLSLIADLIIFGMYIIIILLIKFFKQTQEMADKYKRKNSI
                     RTSLYMLVTFIIIVVNYTTYINFIGVASQNIILINVAIMWLYLILSLYINFTNSALAL
                     KEQQYDQQQDYIRTIDNLINDFRRLKHSYANTIYGFFGYIQEDDLEGLKAYYTEVADE
                     AKRMDSNLLLALQRIKVYAVFGLLWNKINEAEGRGIEVGIRVTNEIREVGMKLTELCE
                     VLGNYLDNAIDAAAASMDKKMNITLTDGGGYLTISIENTYEGDVDVKEIQKKGYSTKG
                     NERGFGLSITNHILSGYTNILHNTFAEDGIFKQELVIKK"
     gene            complement(47339..47476)
                     /locus_tag="PKI50_00240"
     CDS             complement(47339..47476)
                     /locus_tag="PKI50_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HNT02178.1"
                     /translation="MKRFLKPLMILISTLALLIASTSTTACYMWSLREPKMPESLIKR
                     D"
     gene            complement(47479..48096)
                     /locus_tag="PKI50_00245"
     CDS             complement(47479..48096)
                     /locus_tag="PKI50_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HNT02179.1"
                     /translation="MEFPERISGKITKAIAAELKDLSEEKLAEIDYGIAAFIANSYKM
                     VFIFILAMLLNIFQYFLIAFVSFGALRTFASGVHAKREWTCLPTSALVFFGITYLGMI
                     SKLNIYIITFIFILCFGAMLRYAPADTEERPIVSRKLRKKLKILSVITILVLYAIAMY
                     SLGTKASSIITFAVLFESVLILPVTYRLAGSMYGAGMKISNNGEV"
     gene            48353..49165
                     /locus_tag="PKI50_00250"
     CDS             48353..49165
                     /locus_tag="PKI50_00250"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNT02180.1"
                     /translation="MPESERGNLVIIGGAEDKRADKAILKEVIRLSGGKDSRVALITT
                     ATNSPEKSGQEYKKLFGELGAGSVVSINITDRETANNLEGLKVLEDATCIFFVGGDQL
                     RISSILGGTEVHRLLKQAYKNGGVIAGTSAGASMMSEIMVVEGKDDEAPRKCTLKMAP
                     GMGLLEGVIIDQHFNQRGRIGRLLAAVAQNPNVLGIGIDEDTAMIVNKKLEFSVEGSG
                     VVTIVDGRGISFTNISEQYQDDPLAITNVKIHILPRGYGFNLIKKQEIIFRR"
     gene            49206..51833
                     /gene="cphA"
                     /locus_tag="PKI50_00255"
     CDS             49206..51833
                     /gene="cphA"
                     /locus_tag="PKI50_00255"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNT02181.1"
                     /translation="MIIVSSHAYQGKNIYSYKPVVKLVVDLGKYADAPTKDIEGFNDA
                     IQELLPGLKKHCCCKGHEGGFIERLEEGTYFAHVMEHIALELQAMLGYDIRFGKTRET
                     GKKGVYNVIYGYENEYAGLEAGRLAFEIIEEILEGELPDVNTRLEEIKKVCIATDFGV
                     STAAIVKEAQQKDIPVIRLGSGSILQLGYGRNQKRIEATLTENSSCVSVDIACNKELT
                     KSILNEYGIPVPEGKVFKNEQGALEYCEKIGYPVVVKPNYGSHGKGVSINLKNPQEVL
                     EAYKIAREYEDTVLVEKYIKGNYYRVLVVGNKVVAASHRISANVTGDGASTLRELIKR
                     ENSNPMRGEGHEKPLTKINIDKVVEQHLKKRNMTLEYVPDDGEIVYLRENDNLSTGGV
                     AIDVTDEVHEQNKKLIALAAGIVGLDVAGVDISTVDISKPLTETGGAIIEINAAPGIR
                     MHHYPYKGTPRNVAKAIVDMLFPEGSKSRIPIVSVTGTNGKTTTSRMIAHIIKRMGHT
                     VGMTTTSGIYINDELIKYGDNTGPISARTVLMDRRVEYAVLETARGGIVNRGLGYDIA
                     DVGIVTNITEDHLGIDDINTLEDLAYVKSLVAEAVKQNGYTVLNADDQYCLDMRERII
                     SNVILFSLSTDNETVKNHVKNGGIAVYSNGSSIFVNKGGVELPFIEVKAIPAAMNGIL
                     KHNISNSMAAIAGAIGLDIPIESIIAGLSTFRCDSTGNPGRFNIHDINGIKVILDYGH
                     NIDGYRVVIDSLKQMKSGCLIGIIGVPGDRTDSSTIAIGNICGNSFDRIYIKEDRDKR
                     GREPGEIAALLEKGCRMGNIKPSEIFIELAEEKALKKAFLDAVPGDIIIVFFEEYQLL
                     MDVIERIKLDLLKIRQIIA"
     gene            52079..52276
                     /locus_tag="PKI50_00260"
     CDS             52079..52276
                     /locus_tag="PKI50_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02182.1"
                     /translation="MDIHSGDTLVFAVDNMTKRWRISKVDNNVVKIFEEDGSYRQMPY
                     TYLIEMHERGYLKVETPAMVW"
     gene            52436..53788
                     /locus_tag="PKI50_00265"
     CDS             52436..53788
                     /locus_tag="PKI50_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNT02183.1"
                     /translation="MKDNKVETGSYLIIIASLVFILICILFDKPLYFGFAGAVAFTVS
                     VLIKKGYDIRTLNSFMLNGIKGCSRLMVIILLTGIAVSIWLASGVVPTLIYYGFEYIK
                     HANYLLVCFAAAAVVAVVTGTSIGTISTIGIALLGIGKGFEIPAHIVLGAVVSGAFFA
                     DRTSPISGIVNITLKTVNIKYRDYIISILKTLMPPAVITVAVYYFLGRGYISVVNPER
                     IAEFQHSIGSSFVVSPFILLLPLAVIILSLFGINIIFNMSIGIFGGAVLSKFLQNSTV
                     SEVIQYIISGYHPRTGIGELNEILRGGGILSMVELLLILSGAVALNSLLEGANMISPI
                     IQGAVKNVKSKVSMICRTAALSSLITIITCDQVLGVIILGKILQNKFETFGISKIKLA
                     RTIADSGTAIAPLIPWNVNAIIILAITGIPTMQYAPYALMCYLLPVITILFSFFKEKP
                     "
     gene            54004..54840
                     /locus_tag="PKI50_00270"
     CDS             54004..54840
                     /locus_tag="PKI50_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNT02184.1"
                     /translation="MSVKIVADSSCDLNEDLKKELGVTLVPLTLEVGSNVFRDDENLD
                     VKSMLKEMAACPTPPRTACPSPSDFIKAYEGEDSVFVVTLSSNLSGTYESAMLAKKMF
                     LEKAGDKFIHVFDSFSASIAETLISIKIFELAKKKCDDLQIVEKVNEYIESMKTLFLL
                     ESLDNLIKNGRMSRVKGGIATLLNIKPVMGATDDGHIKLVESVRGYRRAFMRFVDIIG
                     EQGEKLEDKVIGIAHCNCLERAEEFKKELLKKYKFKDIIIVEAAGLTSVYANQGGIIV
                     AF"
     gene            54978..55565
                     /locus_tag="PKI50_00275"
     CDS             54978..55565
                     /locus_tag="PKI50_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNT02185.1"
                     /translation="MKRIISAILIVMILSGCSQKPAEPVETAGPAVQPAAEPIVEPEV
                     EPEKTETEQKAEEESNLFDARKAKEGDMVAGLKIIKVQVDSPEMEDYDAYVDFEGEVT
                     VSGTFKHNLNDEFLDHEISFKVDEESEALLPKLAHDQRYVWFMFMNHDEAEKALGAAG
                     TEGKATVTIRNYSINYAHTEIWNTADLVKAEISSE"
     misc_binding    55650..55860
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    55914..56123
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            56177..57070
                     /gene="thrB"
                     /locus_tag="PKI50_00280"
     CDS             56177..57070
                     /gene="thrB"
                     /locus_tag="PKI50_00280"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNT02186.1"
                     /translation="MVEVRVPATSANMGPGFDCLGVALNLYNRFQIEESGNGLLIDGC
                     DETYRNEKNLVYVSMQKCFEKIGCNNRYKGLRINFESDIPISRGLGSSAACILGGVLA
                     ANEIGRGNLNKNEILEIASEIEGHPDNVAPALFGGMTASVKDGNRVYFEKIKLPPGLK
                     FCAIIPDFKLSTKEARAVLPDSIPYKDGVYNVGRVSLLIAALSNGNFEMLKLACKDRL
                     HEIYRGRLIGNYNEIVEECNRLNSLCVFLSGAGPTIMAVLKEEETDFCRQMEEYLSKL
                     ECKWIIKELKSDQNGACVVNI"
     gene            57085..58281
                     /gene="dapG"
                     /locus_tag="PKI50_00285"
     CDS             57085..58281
                     /gene="dapG"
                     /locus_tag="PKI50_00285"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNT02187.1"
                     /translation="MRIIVQKFGGTSVASGERRNLVADKVICAIEQGYSPVVVVSAMG
                     RKGDPYATDSLLSLINDKDCSVDKREADLLMSCGEIISAVVMAEAFAERGYKARVLTG
                     GNAGIITDDNFGNANVVNVEAEKVLKVLEQGLIPIVTGFQGMTEEGDFTTLGRGGSDV
                     TGALLGKALNAEYVEIYTDVDGIMTADPNIVPNAKIIDRIDYREVFELAYQGAKVIHP
                     RAVEYALEDNIPLIVKNTFSDTPGTVITNLVEYEENLITGVTSMTNKAMVIVNPSEVF
                     EGGAEAIFKLLERKKISIDVISESQYQKVFIVDSDAASEVEDILKQLCAEYRIINNCS
                     KIFLIGNKASGIQEVMSKLVKTLEVSNVNVLQTATSHKDICCLIGEADNKKALNELHR
                     AFFCAG"
     gene            58317..59606
                     /locus_tag="PKI50_00290"
     CDS             58317..59606
                     /locus_tag="PKI50_00290"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNT02188.1"
                     /translation="MGKVRIALLGLGTVGQGVWSILNTNKDDILRKCGCEIEVAKILV
                     RDPEKPRGVKVPEGIITADVNDILNDSSIKIVIELMGGTEPAKDYMLRAMKCKKHVVT
                     ANKLVLATQGEELFKTAEQERVLFYYEASVGGGIPIIREINESLTANRIDKLVGIING
                     TTNYILTKMTREGMDFNEALREAQEKGYAEADPSSDVDGYDAAYKLAIMSSLSFGTKI
                     DFSSIYREGISNIKAVDIDYANRFGYVIKLLAIGKEADGRLELRVHPAMVPKDHPLAN
                     VNDSFNAIFIKGNAVGDLMLYGRGAGDLPTGSAVVGDIISILRSNIALSALYSFKNGR
                     AQKEVKSAYENVSEYYIRLNVKDQPGILGEIAAVLGRHDVSIVSVTQDIKDEDNVSIV
                     FITHEALEGNVAESIKEISMLDRVNNLENLIRIENFN"
     gene            complement(59651..59726)
                     /locus_tag="PKI50_00295"
     tRNA            complement(59651..59726)
                     /locus_tag="PKI50_00295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59691..59693),aa:Thr,seq:cgt)
     gene            complement(59746..59928)
                     /locus_tag="PKI50_00300"
     CDS             complement(59746..59928)
                     /locus_tag="PKI50_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02189.1"
                     /translation="MDKTTKKSLIWGGVILVEAFILKLVKKSDLWFYISLPIIAGFLI
                     TEFLIDMPQKPKRNLK"
     gene            60187..60858
                     /locus_tag="PKI50_00305"
     CDS             60187..60858
                     /locus_tag="PKI50_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02190.1"
                     /translation="MKYKYKGSLSLIMIAAFIICAAAGMVSCAPAPTPTPTPTPTPAP
                     EPEPAPEEESLIGFYPLKEGNMWEYEGEGMEYASFIQKVIFSSGNRHQVTIDNGGTII
                     ANVLEESEGSIVNIYRSGEEFSGGNLLDKPSNVNIIMLQTPLEKGNFWISEENHYEII
                     NTEADVVVPAGEFKGCIAVKVTFKDQTSNMTYYYKRGIGMVQSEFRTDSGDLITSKLK
                     RYELK"
     gene            complement(60873..61802)
                     /locus_tag="PKI50_00310"
     CDS             complement(60873..61802)
                     /locus_tag="PKI50_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT02191.1"
                     /translation="MFFKNEESKVMSAFIAVCIIWGSTYLAIRIGVSVFPPELFAGIR
                     FFVAGLLMLIFARLRGLEFPTNLNSIKNQSIVGLFLLLGGNGLVVWAEQWVHSGIAAL
                     LVATAPLFMALIELFIPGRPRIEFKGWLGLLIGFSGVVILVLSSSGVGSINVKGAILI
                     LAASFLWAMGSVYSKSFKPEGSMIVNIGIQMLAGGIALLILGISLGELPRVSISLKGI
                     GALIYLIVFGSLLGYSSYIYILGKWPAAKAGTYAYINPPVAVLLGTLILKEPFTPTVL
                     LATAIILSGVLLVQLSKIKSVDKAVIKEEDPSM"
     regulatory      61930..62047
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            62201..62734
                     /gene="thiT"
                     /locus_tag="PKI50_00315"
     CDS             62201..62734
                     /gene="thiT"
                     /locus_tag="PKI50_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HNT02192.1"
                     /translation="MKRASTQMLAEAGVMIAAAQILSYVKIFEAPYGGSVTAGSMIPI
                     IVFSLRWGVKYGLLAGTAYGILQFLLGGAIYSYHVISILFDYVVAFGMLGLAGLFRSN
                     FKGVFTGTCLGVAGRFICHVISGVVVWASYAPEGMDPMFYSIVYNGSYLLPELIITLI
                     IVGILYKPLISNLGKSA"
     gene            62887..64266
                     /locus_tag="PKI50_00320"
     CDS             62887..64266
                     /locus_tag="PKI50_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02193.1"
                     /translation="MKKMFAVLAALIMVLSGCSGETPGNGTEPDDIDIKNERLVKEFT
                     GTVNIFHRSSDNLLLSAEEGDLFKYYEIDIGTAEVRESKLTEMADRYFYYEPIGNKGF
                     IAVEDTDYKNTLKFIEKDGTEHVIAEDMGPADAINISISPNAGSVAYTSLLEGNDIYG
                     IYVYDTETFENKRLINIKSDGFIEGFHYLVNWSPDENNIIINDKYIYDAFSGSKKGEL
                     KSAYSQWSPTGSKIAFVLEDESGQWLNAEDYHMFQGKKVCVYDIFKGSYDEVFEINGD
                     ECIFGDITWGGKDRFLAFPGIKAGDTDIPDWYMRLNYSSVYIVDLEEGGSKRLETNVD
                     ASDGTMIELGNLKFSNQGNLLSFTVGNYEKSSLHIVNTATLQAESYDNAEYLHWIEGE
                     YYLISAKGDTMYFCMDNSIIGIDENLQEDVKYTSKSKLDDFYMSEDGRGILIFELQED
                     IHIARYVGE"
     gene            64290..65075
                     /locus_tag="PKI50_00325"
     CDS             64290..65075
                     /locus_tag="PKI50_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02194.1"
                     /translation="MYDKVKRETMVKTYFGCNIEDIASIVILTPVWSLESFISKADEV
                     SAEFAGWYKGVTLKFNSKSITVISSGIGAPRSGDCALALSYTDCDTVIFSGSAGAVSQ
                     CCSIGDMLIVSEAVIGEGYSRYHSGDIKRDRFGELVQGDKETAQKLLNAVLKYQSRYG
                     INCREGRVFSIDSLLGERKDTFDYMQEKGCDGVEMEVSAVFTACRCAGKKAAALIIIS
                     DLPLKYKNLFEGIMEIDMKRYNEIEHDLPEILLEAAAGFRQIK"
     gene            65089..65841
                     /locus_tag="PKI50_00330"
     CDS             65089..65841
                     /locus_tag="PKI50_00330"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNT02195.1"
                     /translation="MKFARYESQGIVSYGLVEDSTVYRIEGDIFGEYKVTEERYMLSD
                     VKLLAPCIPSKIVAVGVNYRDHASEMGHELPKDPVLFIKPSSSVIGPEEKIIRPGMSK
                     RVDYEAELALVIGKRTKNVEPSEAFQYVLGATCFNDVTARDLQPKDGQWTTAKSFDTF
                     APMGPFIVTGIDCNNTDIELVLNGETKQKSNTSYLINKTHELVSHVSKIMTLYPGDVI
                     ATGTPSGVGPMSAGDVVEVRLQGIGILRNYVE"
     gene            66126..67085
                     /locus_tag="PKI50_00335"
     CDS             66126..67085
                     /locus_tag="PKI50_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNT02196.1"
                     /translation="MKKILITGALGQIGTELTEYLREQYGNDNVIASNRSKHGDDSFF
                     EAGPFELLNVLDAKGLAEVCRKYGINQIIHLAAILSANAEKNPQTAYDINMNGTYNVL
                     EVARELGIAVFTPSSIAAFGANTPADNTPQDTIQRPSTMYGVTKVAGELLCDYYFKKY
                     GVDTRGVRFPGIISYAALPGGGTTDYAVHIYYEALKNKKYASYIGAGTYMDMMYMPDA
                     LKAVHDLMEANPDKLLHRNAFNVTAMSFEPEMLAAEIRKHIPEFILTYDVDPVRQAIA
                     NSWPNSLDDSAARAEWGWNPEYDLASMTKDMLEKLAVKLNIKE"
     gene            67122..68309
                     /locus_tag="PKI50_00340"
     CDS             67122..68309
                     /locus_tag="PKI50_00340"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNT02197.1"
                     /translation="MGLHDLDFITQRINELKEQGVYRKLPVLESPSGPRCIVNGKNVI
                     NLSSNNYLGLANHPRLREAGKKAIDKWGMGAGAVRTIIGTMSIHEELEEKLAKFKHVE
                     AVLVFQSGFTANAGAIPGIVDKGDVIISDELNHASIIDGCRLSKADVVRYKHSDMEDL
                     ERVIKEVKDNYIIKLIITDGVFSMDGDIAKIPEIVELAEKYGCLTYVDDAHSSGVLGK
                     SGQGSAHHFGLSDRVDVQIGTLSKAIGVVGGYVAGRRNLIDWLNHRGRPFLFSTAAPP
                     AVAAACIEAINILSESTELTDRMWDNANHFKKKLNELGFNTGKSETPITPVITGDDRK
                     AVELSKRLFEEGVFAQAIVFPTVPKGGARVRTIVTAAHTKDDLDEAAAAFAKVGRELG
                     LIK"
     gene            68590..70302
                     /locus_tag="PKI50_00345"
     CDS             68590..70302
                     /locus_tag="PKI50_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNT02198.1"
                     /translation="MRKYFLIILTAFIIAAITGFFVFPQLWTTVQYTPDKVHLVVEDG
                     IAGDEDSPILENGQLLFSIDTIKKFIDPNAHWDEKANKAVFTTKNKVLIMKTGKLTVM
                     VNARPVDINIPVRLVGNKPYIPIQMLDTMFGIEVQWIEENNVAVLDYAKNTVQIAVVQ
                     KDNRIMRAKPALWSPAVRKNIDKGEVLRVFGGDEKWYKVRSKDGFIGYMQKKYVKTDL
                     IASETDMTESAGSTAWKPERGKINMTWEYVGNRNPDVSQLKNISGLDVISPTWFYIVD
                     KQGTVANKADIGYVDWAHKNGHKVWALVGNGSDPDITHDFLNNTDTREKIYQQLLIYA
                     KLYKLDGINIDFENIYMKDRDMLTQFMRELAPLLREQGLVVSIDVTFRSSSENWSMCY
                     DRKALSKVADYVAVMAYDQHWAASPIAGSVAELGWVERGLERILEEVPGEKLLLGLPF
                     YTRVWKEETIDGKIKVSSTAVSMGKVEQILSEKKPEVIWDEESGQYYAEYKEGKATYK
                     IWIEDDKSINLKSSLIHKYDLAGAASWRKGFESEDIWAVLEDNLKVKQNYAQWANANS
                     TAAD"
     gene            70329..72695
                     /locus_tag="PKI50_00350"
     CDS             70329..72695
                     /locus_tag="PKI50_00350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNT02199.1"
                     /translation="MGNEIRISVRDLVEFVYRSGDLDSRFAGMGKAIEGTRLHQKIQG
                     LRKDEAAGSGEIYLKEISVSMRICVRGITFIISGRIDGLLQGSEGITLEEIKTTSSPL
                     DIIGEDTYPVHWAQVMCYAYMYASEQALKTVNVRLTYYNADSGEQKAFIKRADIEELE
                     KHFTEAVEKYYDWAYTACEWIALRNSTIRDMGFPYDKYRKNQRKLAVAVYKTITASKR
                     IFIQAPTGTGKTISVLFPAVKALGEGNTDKIFYLTAKTITRQAALDAISKMKCKELRI
                     KAVVLTAKDKICFKEESDCNPEYCEYAKGYYDKVNAVLKEVFEKEDIITRELVEEYAG
                     RHMVCPFELSLDLSYWTDCIICDYNYVFDPRVSLRRFFTETESNYVLLIDEAHNLVDR
                     AREMFSAELYKKPFLEAKRLMKVKAPLISKAANGINSALLKIRKKSGSKNCCMAKEEL
                     KELCQALRAFISHSEEWLGSNHGEQGYREVLELYFEAAAFLRIYEIFDERYTAYVELP
                     GSDVKLKLFCLDPSKLLGEITGRMKASIFYSATLTPLYYFKDILGGSEEDYNIRLDSP
                     FEESNLSLTVAGNVSTRYTDREGSLSEIADYIKTVVSARKGNYLIFFPSYKYMNEVFG
                     LFTERYPEIGTILQQPAMQEEEKERFLQCFCRDNEKELVGFAVMGGIFSEGIDLEGER
                     LIGAVIVGVGLPQVCFERDIIMDFFKKKNNMGFEYAYMYPGMNKVMQAAGRVIRSESD
                     RGVVLLIDERFCSSRYTGIFPREWLHNNNVESHEELRRILEEFWSRTE"
     gene            72750..73664
                     /locus_tag="PKI50_00355"
     CDS             72750..73664
                     /locus_tag="PKI50_00355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNT02200.1"
                     /translation="MSAISAENMKSFSDTVSDLYTLFFRSYPFMKPFLMNIFLFSSGN
                     FGIIIKEEGREAGRFSVYHSAEGVIVKEGIKEGINVRITLNRKHYEDMMQNRKKYMEK
                     PYKLLKFFPAFLRSVAIDDRSLAENVLAGSRVTLRPGLDSDIYYLLKWYNDIELNKLA
                     GWSNSKATPDKLRYNISRSFGYDPMNLMIDNEEGTPIGTIQLYDFNEQDKSCKLGIRI
                     GDRDYWSKGYGEDAVRTILKYAFFNIDIFRVTLKVYEYNERAVKCYLKCGFRCEGRTR
                     QSACIDGKFYDEIIMGVLKSDFIRLSDK"
     gene            complement(74071..74979)
                     /locus_tag="PKI50_00360"
     CDS             complement(74071..74979)
                     /locus_tag="PKI50_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT02201.1"
                     /translation="MYDILKNCRICPRKCGSDRYEGKGLCGAGSEMLVAKACLHQWEE
                     PCISGGRGSGTVFFSGCNMKCVFCQNHDISQASSGALTGKKISIERLAEIMLELQEQG
                     AHNINLVSPTPYALHIIEAVRKAKEKGLHVPIIYNTNGYELEETIGILKGTVDVYLPD
                     IKYYGDLYSVKYSNTGSYFEYASKAVTAMHNQAGYPEFDKDGMIKKGLIIRHLVLPGL
                     GPDSKRIFQWVRENIGKYAYMSIMCQYTPMHKASQYNDINRRISLSEYDEVLGYFFEA
                     GLENGFMQGKESAHSIYTPEFDLSGI"
     gene            75161..77149
                     /locus_tag="PKI50_00365"
     CDS             75161..77149
                     /locus_tag="PKI50_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNT02202.1"
                     /translation="MTEQDLLFSLDIGTRTVVGIVGFHDGQKYIIKDFEVEEHKERVM
                     YDGQVHDIELVAKAVEKVKERLEEKTGLKLKRVSVAAAGRTLKTCKGFAELDADPSVG
                     VDRELISNLEIEAIQKAQKSMEEQTEEGEQSFFCVGYAVVHYFLNGGIIGNLEGHKAK
                     RIGVEVLATFLPRLVVESLYAVVARAGLEVSSITLEPIAAMNVSISSSLRLLNLALVD
                     IGAGTSDIALTKDGTVFAFAMASIAGDEITEKIAQVLLLDFDEAEKAKISLNNEIIKY
                     TDIMGLIHEKNSSEVLNEIDESIRILAEEISNKILEFNGKAPSAVFLVGGGSQIPRLT
                     GYIAEYLRIPSERVGVRSTDIIRNVEIESKGLSGPEFITPIGIAVTSYFNRQKDFLHV
                     TVNGVNVKMLNTKALTIADSLIMMNYNPRNLIGRRGNPLTFKVNGREVIVRGEPGVPA
                     VIYLNNNIAGLDRPITNGDEIIVKPAVEGKPAEANVKDYLGDYIEKTVYLNENPLNLR
                     PMITINGLECNLDAPIKNSDEVNIENIDTVHELLKSLNLNYNNIEVEINGKKAEPGDV
                     INDGDHISYKVLERTETESFRKPDKKTVCVNVTINGKPEVLVTNKTQCLFVDVFNNIE
                     IDFDSIKGVSAMLLNGNKASFTDVINDGDVIELHWKEK"
     gene            77253..78638
                     /locus_tag="PKI50_00370"
     CDS             77253..78638
                     /locus_tag="PKI50_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNT02203.1"
                     /translation="MSRSMKIGIGVIIVLLIAAAALSAAMVSGDKIHSNISINGIDVS
                     GFVPSQAESMLKKKMEPIIDNLSIVLKFKGKEWQLKYRDIDFKYEYEDAVTKAYDIGH
                     KGSYFKRIKDVAATQLNGEDIKLNYSYDSQYLKNRLEDISKELEQEARDASIVLKEQG
                     FVITDEVEGRKLDVNKSCDIIKALLDKASEGEAELAVDVTEPEIKRSDLENIKDNIGE
                     YSTKFNAADVDRTHNIKIAASSASHILVKPGEVFSLNKSMGPRLAKFGYKEADVIINN
                     ELVPGIGGGVCQVSSTLYNAALLSNLKIVERKSHSLPSSYIALGRDATLSEDYIDLKF
                     ENNTKYPIYIYGEVKGSWVKFSIYGYDEYPERTVKIKTETIKVTPPQISIIEDPALPE
                     GTEVEEKKAYTGYVVKSYRVVMENGKEVSTEELPLSVYRVVNGVKRVGTMKVEPQPAE
                     NEVLPEVPGDI"
     gene            78692..79378
                     /locus_tag="PKI50_00375"
     CDS             78692..79378
                     /locus_tag="PKI50_00375"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNT02204.1"
                     /translation="MNDSLYSKKAICPICGKQFFSMKAKVNSCKVKKKDEDFCIHYED
                     LNPMYYEVFVCPSCAYAAPENSFKELTEKEVKLLKEVFSGREVSRSFCGQRSLDDATA
                     AYKLAIHTAELRKASASVLAGLCLRLAWLYRFKGDKQEQLFLEYSLRNYLEAYDKEPL
                     PIGNLNEISLMYLLGELSRRLGKLDDAITWFEKAATNPEKKENPMIEKLAREQWALTR
                     EQHKESEKSE"
     gene            79661..80611
                     /locus_tag="PKI50_00380"
     CDS             79661..80611
                     /locus_tag="PKI50_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT02205.1"
                     /translation="MIDKDLFIKKIEESVSKVIVGKKDVLEKLLVVLFAGGHVLIEDA
                     PGVGKTTMVHALARSTGCSFKRIQFTPDLLPSDITGVTVYNIKQGEFEFKHGPITSNI
                     VLADEINRGSPKTQSSLLEAMQERQITVDGNTWKLPEPFMVLATQNPIEYEGTFPLPE
                     AQIDRFAIRISMGYPTYNEEKSILNKCSSDKAPENIEPVISAEEVLEIRRLVEKVHAD
                     DSVQDYIVKLVRGTREHKDVYLGCSPRGTLALFSNCRALAYIRGREYVLPDDVKELVF
                     CTLAHRIILKSEAAIQGKDQYSVLAEILKSIKVPVANING"
     gene            80604..81791
                     /locus_tag="PKI50_00385"
     CDS             80604..81791
                     /locus_tag="PKI50_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT02206.1"
                     /translation="MVEMNRKALALILLIFAAALFAGGIFMYGIFLTALFLAIISYAS
                     GRYIYNNLVNLTWEISSNAVRGDRVSLKTDWYNAGIIPIPYLKVIAGLPKRLAGEEQK
                     QRVYSIMPEMITTMSRDFECRHKGVYKIGIIETVFEDTLGIFKWKKVFYDDRYLVVYP
                     KVHLLKHLDIPMRQQYGTSAVKHAAYEDCTGTKDIRKYIIGDSFKKIHWKVTAHRGDF
                     YVRNVEINASADLIVFLDLYDYGFDEETAYDIEEKGAECAASIIRYALANSMSVNFTA
                     KGEELVSLSAKRADRFNLFLDAVSKFSSKGDIPIGDLVRKEARKLDWEATAVVITPCI
                     EKNASAFLSLKASGTELVIIYLCGDGANGGKDAEILKSSGLNVFAVGLEDDINQVLGG
                     HYE"
     gene            81784..84048
                     /locus_tag="PKI50_00390"
     CDS             81784..84048
                     /locus_tag="PKI50_00390"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HNT02207.1"
                     /translation="MNNRNIKEILFYFSLIFMMVFSFNLALKDFVGIDMHLIGVFLIS
                     LSASASASMLLLFPAAVSAGLIPAAGFLLFYCYKEPASVRLYLKEAQEFTTWLLGYLA
                     GYNYFEKAYSLLFIIVITLLAALITSTAVYKGKGVLPLILAGTAVMAYFWFVYVESAR
                     LYLALFLFASIMLYSYRTFKKRLREWIAAGCIIDYKAGRNWMICSGVVIAAALLFSAA
                     LPLKIGPVRWQWLNDKAVSLFPFITEWRNDVLESYSFSSAGYSDNKLGGEIRWDDSVV
                     MLVRTRGEEALYLRGTVMDRYYENSWNKSGKRYKEYKPGHLMPIPYGKDIKVYTRDLE
                     ISHRKLLTSTIFAPYSLYRIQHGGKRTYMDEDSEAYASKIAATDTSYRVTSIIPYIDV
                     AKLRLSGAETLEEDDLRRYTALPEDIPERVRSLSHRITNAYSSNYDKAKAIEKYLRQN
                     YMYSLKPPQLPSNKEFTDYFLFEGKEGYCTYFATSMAVLLRASGIPCRYVEGFIASYE
                     GSEEREVRGTDAHAWVEVFFDGYGWVTFEPTPQYPVVEILEPGSVKDEVFEGAANDTV
                     TVENKRAGMPGYFGQPEGAERVGNGTISGDIKKSYNFAKIILLIALSALTARVIFLYV
                     KQAITEIGLGRSSGKRFAADYIEDINRYLRRAGFEMKGSETLREFMNRVGLSFRENPG
                     HISKIITILEKTRYGGYEPCANERSILKVFRKEVKAFAIKKAGILRYFISMYVLGDRK
                     NAVQKFHVEFKKIT"
     gene            84065..84151
                     /locus_tag="PKI50_00395"
     tRNA            84065..84151
                     /locus_tag="PKI50_00395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84099..84101,aa:Leu,seq:cag)
     gene            84321..84926
                     /locus_tag="PKI50_00400"
     CDS             84321..84926
                     /locus_tag="PKI50_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNT02208.1"
                     /translation="MGSVRVMPDSTCDLSPELIGKHGISVVPLYVIFNGDSFRDGIDI
                     KTDELYKMTERLNTLTVRNARITAQEFPEGRIEVIDSRNLSAGIGLLVMKAVDFAGQG
                     LGISEIEEGKMILEQKIRGKKEKALKSMPENTLTDKSIIDSRSVFVTHSMADEEAEYL
                     KGVLEQKLEGPEIIITNAGCVISSHCGRGTAGILYMESSEA"
     gene            84953..85786
                     /locus_tag="PKI50_00405"
     CDS             84953..85786
                     /locus_tag="PKI50_00405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNT02209.1"
                     /translation="MMNRDSLKYKMLVTDMDYTLLNKEKRVSDRNREALRRAMEKGVH
                     MVVATGRIYTSARIYAKLLGLDTPIIASNGALIKDASKTIFRDILSQDTVREMLRLCH
                     KYGIYCHFFTENTIYSEKLINVSLRYTEWNKYMGEEDQVKIRIVDDGEEIIEAAKSEV
                     LKAVVFDDDDEKIQKLRDGIMETGIVSVSQSMKHNLEVMNKGVTKGNAVRILAQLYGI
                     NREEVIAIGDNENDISMIEYAGLGIAMGNAEECLKRAADHVTGDYQEDGVAEAIEKFI
                     L"
     gene            85880..86701
                     /locus_tag="PKI50_00410"
     CDS             85880..86701
                     /locus_tag="PKI50_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNT02210.1"
                     /translation="MKKLFLFIAAVLAMGYMSFISMSIPAVLEKKSIEYGNDGSAANT
                     MPEIRVMTYNIHRGINKENKLDLNGISEAIRDSGAEIIALQEVERFSVRTGFRDQIKY
                     IADKLSMKYAYGKSINILNGQYGNAILSRFPIEEYEVCKLPSDGERRTILRAVLNVYG
                     NRIHFYSTHLGLDQEERDIQAEEIVRLAEEDRNSIVSGDFNSKADKLGMLTERYMDCA
                     SYENDDIRVTFEGEGLSERIDYIFVSRNFRVKSYEVLKSDASDHYPVVCTLDFID"
     gene            86778..87611
                     /locus_tag="PKI50_00415"
     CDS             86778..87611
                     /locus_tag="PKI50_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HNT02211.1"
                     /translation="MPFNPKRVLFEQDALKYPLGRELFDYFKGRDVQTGMLKSHNRVT
                     DIPGKTPAQAYSEGKNTLVVGVRRTLNFESCKPSAHYQLPLATGCMGMCMYCYLNTQL
                     GKKPYMRVYVNVEGILEAVHNGHTTVRFSLNAANVIKKYEHRTPEAEERVSAARKIAG
                     HDYPLGFIIAPVLLYEGWKKDYGELLNMLKDKLDTYINRSICFEIISHRFTSRAKKNI
                     SEIFPGNDLPMNEEERRFKYGQFGYGKYVYNREQLSDMESFFRERIAEIFPNSRIDYI
                     I"
     gene            87706..89409
                     /locus_tag="PKI50_00420"
     CDS             87706..89409
                     /locus_tag="PKI50_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT02212.1"
                     /translation="MNRSFKRISWLLTVVMILAMLLPMGGVWADESGAAAAGSVNLTI
                     VHTNDTHSRVLSSSSEIGFAKIASKVKEIRSSNENVLVLDAGDTFHGQTISSLVEGES
                     VVEVMNIIKYDAMVPGNHDFNYGQERLLELSQMTNFPIVAANITKADGSAFLSPYIIK
                     EFKGVKVGIFGLATPETTYKTNPNNVKGLTFEDPTAAAERMVQELEGKADIIIALAHL
                     GLDESSEYTSKLVAEKVDGIDLIIDGHSHTVLTEGLKVGDTLIVQTGEYDKNFGIVEL
                     ACENGSVTAAKASLITIEAAEQLPEDAEVLQAIEGIKAEQDVITSVVIGRTEVELDGV
                     REHVRAGETNLGNLITEAILKATGADAVITNGGGIRASIDTGDITKGEVITVLPFGNY
                     VVVKKIKGSDIIAALEHGVSQYPAPNGGFPHVAGIRFAFDPAAAAGSRITKAEIGGKA
                     LDPNAEYLLATNDFMAVGGDNYTMFAPGPVITELPGLDEIVMEYIANYGTADIKVDGR
                     ILAEEKKAQAAAEYEVRLGDVLWKIAEKFGLTYEKLAEYNKLKDANRIYPGQKLLIPE
                     F"
     gene            89541..90008
                     /locus_tag="PKI50_00425"
     CDS             89541..90008
                     /locus_tag="PKI50_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNT02213.1"
                     /translation="MNVEYINPFVNVSVNLMKMICNETATRGKIYIKDSTFSSDNVVI
                     IIGVAGEIKGQVFFSMNNTTACNIASTMMFGMEVKELDDMAKSAIAELGNMIMGNVST
                     EFFNNGTKIDITPPTILVGKEIAFSTKGLKTICIPLVLEKLGRIEIDVALTEY"
     gene            90030..90494
                     /gene="trmL"
                     /locus_tag="PKI50_00430"
     CDS             90030..90494
                     /gene="trmL"
                     /locus_tag="PKI50_00430"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNT02214.1"
                     /translation="MSLNIVLVEPEIPQNTGNIARTCAATGSRLHLVKPLGFSVDDKH
                     LKRAGLDYWGLVNIYYYDSFAELKSRYSDGLFFYSTTKAKKKYTEVIYKENCFIVFGK
                     ETAGLPKLLLEENKEFCIRVPMINNARSLNLANSVAIVAYEALRQIGFPGMH"
     gene            90518..91357
                     /locus_tag="PKI50_00435"
     CDS             90518..91357
                     /locus_tag="PKI50_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNT02215.1"
                     /translation="MGIKIITDSICDVPKDYADRYGIRVMPLRVNFGNTSYIDGIDLT
                     LEEFLKKLEGAEVLPTTSQVTPGDFLAAYREETALGNKVISIHASSQLSGTHDSAVIA
                     KEQMPGSDIYVIDTMGISLGAGLLAIKAARLAEEGFEAEEVVKQIEASKQKMKYMLIL
                     DTLKYLQKGGRLSLPASVIGSILSIKPILTVVNGKLELLEKSRGMKKAIFSVMNRIKE
                     NGWSLNGKTVGINHITNLESRDILEEQLKKEFNIKEFIRGEAGTVIATHGGPGAVAVH
                     FEI"
     gene            91369..92472
                     /locus_tag="PKI50_00440"
     CDS             91369..92472
                     /locus_tag="PKI50_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT02216.1"
                     /translation="MKSKVYFAGVHSNAGQESLLVKLRKLFDRAGFGEMISQNDIVAV
                     KIHFGEKGNTAYIRPTYARQIVDKIKEKGGKPFLTDSNTLYNGSRANGVDHYETAIYN
                     GFAYAVVNAPIIIADGILSKNSVDVEINKKHFDRVKISKEIASASKMIVMSHYKGHEN
                     AGFGGAIKNLAMGCAPAAGKQQQHSVVRPKVSRRCVGCENCLKYCPAGAITKEGGHAL
                     IGETCIGCGECISICPIRAINPQWDIEIPVFIERMTEYAYGAVKNKNGNAGFINVLMN
                     ITPLCDCYGFSGAYIVPDIGILASKDPVAIDAASYDLVNEQTGNINSSLKCNHGKGED
                     KFRGLYGNIDGTRQLSYGEEIGLGSREYELIKI"
     regulatory      92577..92675
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            92863..93228
                     /locus_tag="PKI50_00445"
     CDS             92863..93228
                     /locus_tag="PKI50_00445"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HNT02217.1"
                     /translation="MLTNNSLVRDEYGKDLDIIFIDGEYIDVLKSARDMVHKGAKLIT
                     HPLMGSVKPNETPFRSIIVAQGDSLDIGSLTVMESSIASAEKFQRDVKVPLWNDRVLN
                     DFRFVDLKLFESAYDSITK"
     gene            93243..94175
                     /gene="trxB"
                     /locus_tag="PKI50_00450"
     CDS             93243..94175
                     /gene="trxB"
                     /locus_tag="PKI50_00450"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNT02218.1"
                     /translation="MGKVYDVVIIGGGPAGLSAGLYASRARMSAMIVEKAKWGGQAGT
                     TEELENYPGSIEMPTGPKLMERMKNQAEAFGTELVKDEVLSIDVSDKIKKIKLGSGNE
                     ISARTIIIATGAQPMMLGIPGEAELRGKGVSYCATCDADFFAELEVVVVGGGDAAVEE
                     AIYLTKFAEKVTIVHRRDQFRAAKSIAEKAMKNDKIAIMWDTVPEEIYGDGIVEGIKL
                     RNVKTNEVTDFRTDGVFMFVGTKPISDFAKGVVEMDQKGYIMADEEMRTSVEGIFAAG
                     DVRVKSLRQVVTAAADGAIAAVNAEKYIEKNFEN"
     gene            94189..94509
                     /locus_tag="PKI50_00455"
     CDS             94189..94509
                     /locus_tag="PKI50_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT02219.1"
                     /translation="MIELDKDNFDSEVLQATGPVLVDFWGEKCEPCKALMPEVHALAE
                     KYGDKMKMCKLNTTQNRRLAIGQKVLGLPTFIVYKNGEKIKEISGAENCTPEAVENLI
                     KENL"
     gene            94569..95855
                     /locus_tag="PKI50_00460"
     CDS             94569..95855
                     /locus_tag="PKI50_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HNT02220.1"
                     /translation="MRLELGNIFIKDVQFGDATKVEKGTLYVNKAELLEAVSGDPRLT
                     KIEIDIAKPGEETRIMPVKDVIEPRVKVEGPGGIFPGMVSKVDVVGSGKTNVLKGAAV
                     VTAGKIVGFQEGIIDMSGPGAEYTPFSKTQNIVIVCTPVEKLQQHEHEHAVRMVGYKA
                     AAYLGEAGRKVVPDEIKVYETPTLMEGMKQYPNLPRVAYVYMLQTQGLLHDTYVYGVD
                     AKKIVPTFIYPTETMDGAIVSGNCVSACDKNPTYNHLNNPIIEDLYERHGKDFNFVGV
                     IITNENVTLADKERSSNWTAKLAEYLNLDAVIISEEGFGNPDTDLMMNCKKIAAKGIK
                     TVLVTDEYAGRDGASQSLADADKAADAVVTAGNANEIISLPPMKKVIGYLEPADTIAG
                     GFAGSLKADGSITAELQVITGSTSEIGYTKLSAVEY"
     gene            95875..96333
                     /gene="grdA"
                     /locus_tag="PKI50_00465"
     CDS             95875..96333
                     /gene="grdA"
                     /locus_tag="PKI50_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_081427418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:95998..96000,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HNT02221.1"
                     /translation="MLQDKKVIIIGDRDGIPAPAIEECLKTTGAEIVFAATECFVUTA
                     AGAMDLENQKRVLDLSEKYGKENVVVILGTSEAESAGLTAETVTAGDPTYAGPLAGVS
                     LGLGVYHMFELKDQVDANVYDEQCGMMEMVLDVDAIIKEVSSIREQYSKY"
     gene            96398..97702
                     /gene="grdB"
                     /locus_tag="PKI50_00470"
     CDS             96398..97702
                     /gene="grdB"
                     /locus_tag="PKI50_00470"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:97439..97441,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HNT02222.1"
                     /translation="MIRVVHYINQFYGGIGGEEKADVKPEIREGVVGPGMALKAALGN
                     EADIIATVICGDSYFNENMEEASAAILEMVKKYNPDLFIAGPAFNAGRYGVACGSICK
                     AVKEKFNIPVVTGMYIENPGVDMFKKDVHIVETANAATGMRQAIPAIAKLAMKLAKNE
                     SIGAPQEEGYIPRGVRKNVFKSERGSKRAVDMLVAKLKGEKFETEYPMPDFDRVAPNP
                     PILDIKKAKIALVTSGGIVPKGNPDRIESSSASKYGKYDLTGVNDLTAETYETAHGGY
                     DPTYANIDADRVLPVDVLRDLEKEGVIGELHRYYYATVGNGTSVANAKKFAATFAQEL
                     VKDGVQAVILTSTUGTCTRCGATMVKEIERVGIPVVHICTVVPISLTVGANRIVPAIA
                     IPHPLGNPAMSKEDEKALRRSIVLKALKALETEIHGQTVFEK"
     gene            97762..99306
                     /gene="grdC"
                     /locus_tag="PKI50_00475"
     CDS             97762..99306
                     /gene="grdC"
                     /locus_tag="PKI50_00475"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HNT02223.1"
                     /translation="MTAPVVKGAAYVLVHTPDMIMHYGTTCALEHEANPESEFLKKFP
                     EFVRSYEEAVKYAPNQVYIGNMLPDELNTIEKPWYKNLVQDADGEGKMGEIIDQDELY
                     VALKLSDAFDLVALEKTFLEDTKAKLAKKPMYTEAELAKLKEGAEQSEIDSRVKEGAE
                     PLYMGKKLVGCVKAAHDKDKNLTAHIMVENLVVKASGVIALKNLIYRNNIPADSIEYV
                     IECSEEACGDMNQRGGGNFAKAVCEAAGATGATGSDTRGFCAAPAHALINAAAMVKSG
                     VYKSVAIVAGGATAKLGMNSRDHVNKGVPVLEDCLGGFAILLTENDGISPVLRTDTVG
                     RHTVGTGSSPQAVMESLITKPLDKVGMKITDVDKYSVEMQNPEITESAGAGDVPLANY
                     KMIAALGAKRGDIDRTEMMSFIKEHGMPGFAPTQGHIPSGVPFVGAARDMIMSGAIER
                     AMIVGKGSLFLGRITNQFDGVSIIMEKNTGREDSAGTVSKEEVKEMVAEAMKDFASFL
                     LNGRQG"
     gene            99312..100499
                     /gene="grdD"
                     /locus_tag="PKI50_00480"
     CDS             99312..100499
                     /gene="grdD"
                     /locus_tag="PKI50_00480"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HNT02224.1"
                     /translation="MSDNQVKSMIGEVFNEIADAIMTGEFGKRVKVGLTTLGSEHGTQ
                     ELVKGAEAAAKKYGDFEVVLIGPKVDTELRVVETDNEEEMHKKMDELLDNGELDAALT
                     MHYSFPIGVSTVGRVITPGLGKELILATTTGTSSTHRVEGMVKNALHGIIAAKALGIK
                     NPTVGILNVDGARQVERVLKELKSNGYDINFSESVRADGGVVMRGNDLLSAAADIMVT
                     DTLTGNLMMKIFSAFTTGGSYESLGYGYGPGVGDNYGRIIGILSRASGAPVVCEGLRY
                     MASCAKGGLTKIAREEYEKARKAGLDSILKELTADSAKAAPKSEVKQLEKKPVTKSIA
                     GIDILELENAVSSLAEEGIYSESGMGCTGPIVLVAEEDYIKAVDALLKNKHITERPLD
                     CNC"
     gene            100768..101832
                     /locus_tag="PKI50_00485"
     CDS             100768..101832
                     /locus_tag="PKI50_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT02225.1"
                     /translation="MLKKGLILVLAIVMVMTMFTGCAQPAQAPAEPAQEAEQAPAEEA
                     KPELSFGMITDVGGLGDKSFNDSAWEGLQKAGKELGVEPVILQSTKQEDYEANIGNMA
                     QSVDLSIAVGFLMEQAMISGAEQYPDMKFGIIDMVVPKPNVMSVTFKEHDGSFLVGVI
                     AGLTTKSNIVGFVGGMEGPLIEKFEVGFRAGVASVNPDAKVLVNYTGAFDNPDKGKEI
                     AISQFKQKADVIYHASGACGIGVIQAADENGFWAIGVDQDQSAVSKNNKVLCSMIKRV
                     DVGTYTIVKSYADNNFAGGKAIELGLKEDGVGFSDGGNNVPAEVKAVADKAKELVVNG
                     TIVVPSTKKELEAFKPVEIK"
     gene            102054..103574
                     /locus_tag="PKI50_00490"
     CDS             102054..103574
                     /locus_tag="PKI50_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02226.1"
                     /translation="MPAVIEMKGISKSFPGIRANDNVNLKIEQGEIHVLLGENGAGKS
                     TLMNILYGLYQPDEGQIFIKGKPVKIVNPNVAISHGIGMVHQHFMLVQPFTVTENIIL
                     GKEPKKGAALDIDKAIKDVEEISKKYELKVDPRAVVQDISVGMQQRVEILKTLYRGAE
                     ILILDEPTAVLTPQEIEELCIILRSLVDQGKSIILITHKLKEVISMSDKVTIIRRGKV
                     IDTLNTKDTNIDELAEKMVGRKVSLTVEKKEAEVSAPILEIKDLRVLNNRKLEAVKGI
                     DLQVHGGEILGIAGVDGNGQSELVQAITGLSKAEAGEILLAGKDITNLTPKEVIASGV
                     GHIPEDRHKRGLVLAYSLAENSILGSHHMKQFRQGFGLNYQKIREYCRRLIKEYDVRT
                     PNERVLAKSLSGGNQQKLIVAREIDRDPKLCIASQPTRGLDVGAIEFVHKKLIELRDE
                     GKAVLLVSLELDEIMDLSDRIAVMYDGKIVGILNAKDATEKKLGIMMAGGSAQDAV"
     gene            103587..104699
                     /locus_tag="PKI50_00495"
     CDS             103587..104699
                     /locus_tag="PKI50_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02227.1"
                     /translation="MESGINEFKEGLLNTLKEIAYPLVAILISVIAGGILVYSFGSDP
                     IIAYTAMIKGSLGDWNKTGETLVTVTPLILTGLSIAFAFKCNLFNIGVEGQFIIGSIT
                     AVWAGWAFAGLPAILHLPLTLLAGTVAGGIWASVIGWLKAKVGSHEVINGIMMNYIAM
                     YTSNFLVRTVLNVPGKAYSIEIADTAKLWRFSQAFEQFNHSRLHIGIILSLIVALIAY
                     YILNKTVRGYEIRAVGFNPHAAEYGGISVRKNIILAMIVSGLFAGLAGSIMATGVQYR
                     VNNLFGFTGYGLDGIAVSLVGNNHPIGIILSAFLFGVLQKGGPLMQIQGIPKEVVGII
                     QGIIILFVAANFVKVVMENIKLNKDLKRTAVKEEVE"
     gene            104701..105591
                     /locus_tag="PKI50_00500"
     CDS             104701..105591
                     /locus_tag="PKI50_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02228.1"
                     /translation="MEGVGILTIIGIVVTASIRSATPLIFASLGGNFSERSGVFNIGL
                     EGIMTVGAFSAVFFSYKTGSPWAGLLGAMVVGGLFGLLLAFMSIYFKANQVIVGTAIN
                     ILAASLTTFLLVEIWGRPGQTDNVEYFKAVGPFNLFTYLAFVMVIVSYYVMYKTPFGL
                     RVRAVGEHPRAADTLGVNVYVIRYICVMISGVLAGIGGASLSIGSMSLFKEGMIAGKG
                     FIALAALIFGKWNPIGAALACLFFGFAVALETVATNLPVPREFLYALPYIMTMFAVSG
                     FVGRAVGPAAGGIPYEKGEK"
     gene            105766..107169
                     /gene="rpoN"
                     /locus_tag="PKI50_00505"
     CDS             105766..107169
                     /gene="rpoN"
                     /locus_tag="PKI50_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNT02229.1"
                     /translation="MRMGYDLLIEQQQKLIMTPELKLALKILQLPAVELEELLQQELE
                     DNPILELADDNRDEKSEVQQKQDKKEKEKEKEIDWKEYFQFQGKSYAEEGFDSDEAAE
                     LSYENFITYSYTLKDHLISQLRVSQISKKLRDIGEYIIESLDENGYLTITKEDLAAIL
                     DVKPEEAEEALSVIQTFEPFGVGASNLKECLLIQLRNKDIYDKKIEVIIINHLDDIAS
                     NRINNISKKLSISIEEAQKYSDIIKNLEPKPGRAFEEGTSTKYVTPDVYIEKVGKEYV
                     VIINDYYGSKLMINQFYKKMLISEDKSSQASAFISNKLSSAMWLIKSLEHRKNTLYNV
                     VKAILNYQMDFFEKGPMFLKTMTLKKIAEMVNVHESTVSRAINGKYVQTPRGIFEIKY
                     FFKSGVDNQDGEAISSESIKKMIKSYVSGEDASKPLSDQHIADLLVKEGFMISRRTVA
                     KYRDELGILSSSKRKRY"
     gene            107344..108375
                     /locus_tag="PKI50_00510"
     CDS             107344..108375
                     /locus_tag="PKI50_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HNT02230.1"
                     /translation="MNEFLNLQKRLVPEIIEITEKRYSILRAIEFNQPVGRRALAGIL
                     GQSERWVRSELDILKSQNLIYVQALGMVVTDEGNEVINKLRDYISELKGLKDLEKAVR
                     EMLNIRELIAVPGDTENELQVVVEMAKAAFEYIKGIIEDNDIIAVSGGYSALSVAENA
                     RRIDAQNITIVPARGGIKTDMERQANIVAEKLAKNIGGNYYLLHLPDNINEDMLKRMA
                     DDPDIAKVLNYIAETDVLIYGLSSFESISRKRNFTEEQVEELKTLNVRAEAVGCYFDS
                     NGNIVYKSGSIGIKPEHLDSIKTVMSVGGGARKAEAIVLTSKGRKNRVVVTDEAAARE
                     IYKILKGGN"
     gene            108377..109384
                     /gene="gap"
                     /locus_tag="PKI50_00515"
     CDS             108377..109384
                     /gene="gap"
                     /locus_tag="PKI50_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNT02231.1"
                     /translation="MALRVGINGFGRIGRPVFKIASEKFGDDAEIVLINDLTDTKTLA
                     HLLKYDSTYGRYDRDVQAKEHSLVIDGKEIEICAETKPENIRWGDYGVDVVIESTGRF
                     TSKEAAMAHVAGGAKKVIISAPAKNEDITIVMGVNEDKYDPEKHVVISNASCTTNCLA
                     PVAKVLHENFGIVKGLMNTIHSYTNDQRILDLPHSDLRRSRAAALSIIPTTTGAAKAV
                     ALVLPELKGKLTGLSMRVPTPTVSVVDLVVDLERNVTVEEVNAALKRAAENELKGILE
                     YCDEPLVSIDFKKDPHSSIVDALSTMVIQDNMVKIISWYDNEWGYSNRVVDLVKYISN
                     K"
     gene            109408..110592
                     /locus_tag="PKI50_00520"
     CDS             109408..110592
                     /locus_tag="PKI50_00520"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT02232.1"
                     /translation="MNKKSIQDIDVDGKKVLVRVDFNVPLDENGTITDDRRIREALPT
                     IRYLREHNAKVILMSHLGRPKGKFNMKYSLKPVAERLSGKLGAEVRLAGDVIGEEVSA
                     MVNSLKEGDVMLLENVRFYEEEEKNDKEFSGKLAALGDIYINDAFGTAHRAHATTAGI
                     AEYLPSAVGFLIKKELEIMGKALTAPERPFVAVLGGAKVSDKIGVIENLMDKVDTLLI
                     GGGMAYTFFGAMGYETGKSIFEADKAELAGELLKKAETKGVKLLLPVDTVVASEFSND
                     APKKTVPSAMIPKDMMGMDIGEDTRKIFSEIVKNARTVLWNGPMGVFEMPNFAKGTFE
                     LAKAMAECTGTTIIGGGDSAAAVEQLGFADKMTHISTGGGASLEFIEGKVLPGIAAIE
                     NK"
     gene            110609..111361
                     /gene="tpiA"
                     /locus_tag="PKI50_00525"
     CDS             110609..111361
                     /gene="tpiA"
                     /locus_tag="PKI50_00525"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNT02233.1"
                     /translation="MRRPVIAGNWKMNKTVNEAIKLAEELKPLVKGAAAEVAVCPAFI
                     CLNDLVKVLKGSNIRVGAQNMYHKESGAYTGEISPVFLKDIEVEYVILGHSERRQYFR
                     EEDSEINKKVIAALDHGLRPILCVGETLEQREAGVTFDVIAAQVKGCLEGLENRNTDD
                     LIIAYEPVWAIGTGKTATSQDANEVIGFIRKQLALLLGNDAADKTRILYGGSVKSSNI
                     KELMDMPEIDGALVGGASLDAQEFSRIVNFNQ"
     gene            111393..112676
                     /gene="eno"
                     /locus_tag="PKI50_00530"
     CDS             111393..112676
                     /gene="eno"
                     /locus_tag="PKI50_00530"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNT02234.1"
                     /translation="MTVITDIYAREIIDSRGNPTVEVEVELEGGALGRAAVPSGASTG
                     AFEAVELRDGDKSRYLGKGVLTAVNNVNEIIAPEIIGMNALDQVAIDQALIELDDTPN
                     KGKLGANAILGVSLAVAHAAAEALGLPLYQYVGGVNAKTLPVPMMNILNGGKHADNNV
                     DIQEFMVMPVGAESFKEALRMCAEVFHNLKSVLKAKGYNTAVGDEGGFAPNLTSNEEA
                     LQVIIEAIKKAGYEPGKDLYIAMDAAATELYKEDGKYHLDGEGVVKTSEEMVDYYENL
                     VSKYPIISLEDGLSEEDWDGWKLLTERLGKKIQLVGDDLFVTNTERLEKGIEMGVANS
                     ILIKVNQIGTLTETLDAIQMANRAGYTAVVSHRSGETEDVTIADLVVAVNAGQIKTGA
                     PSRTDRVAKYNQLMRIEEGLDTMSEYLGVNAFFNL"
     gene            112945..113175
                     /gene="secG"
                     /locus_tag="PKI50_00535"
     CDS             112945..113175
                     /gene="secG"
                     /locus_tag="PKI50_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNT02235.1"
                     /translation="MRLAIVILQYVVCLVLIVSVLLQPGRSAGLSGSIAGGAEQLFGK
                     KASKGYEALFSKLTTASAILFMVLSVVLVLVK"
     gene            113280..115244
                     /locus_tag="PKI50_00540"
     CDS             113280..115244
                     /locus_tag="PKI50_00540"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNT02236.1"
                     /translation="MDLLILAPLSGVLALVFAWFLANSIAKKDPGNDRMKEIMGFIHE
                     GAMAFLFREYKVLVVFIIVLAIIIGVFLKSWLTAICFVAGACCSILAGYFGMQVATKA
                     NARTANAAQRGQNEALGIAFRGGAVMGMCVVGLGLLGLGTLYIFIRDASIVTGFGLGA
                     SSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMG
                     ADLFESYVGSIISAITLGAIVYAGKVEGVLFPMLLAAIGIVASIIGTSFVKTDEKSNP
                     QSALSRGTYGSSIIVIVASFILSRYMFGNLNAFWAVAAGLVAGSIIGFVSEVYTSSDY
                     KYVKKIAQQSGTGSATTIISGLGVGMLSTVAPIIFICAAVLVAYDSAGIYGIALAAVG
                     MLSTTGMTVAVDAYGPIADNAGGIAEMAGLPESVREITDKLDSVGNTTAAIGKGFAIG
                     SAALTALALFVSYSEAAGLKTIDILNPKVMVGMFIGGALPFLFAALTMEAVGKAANQM
                     IEEVRRQFRETAGIMEGTTKPDYARCVDISTAAALREMIVPGLLAVIVPIATGFILGK
                     GALGGLIAGAVVTGVLLAIMMANSGGAWDNAKKYIESGKMGGKGSVAHKAAVVGDTVG
                     DPFKDTAGPSMNILIKLMTIVALVFAPLFM"
     gene            115386..115655
                     /locus_tag="PKI50_00545"
     CDS             115386..115655
                     /locus_tag="PKI50_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HNT02237.1"
                     /translation="MKTLLIYLITVNIAAFAAMGADKYRAQRNKWRISESSIFALGLI
                     GGGAGIYLGMKLFRHKTRHLEFTLGIPVIIILNAIALWYILQKLE"
     gene            115862..116644
                     /locus_tag="PKI50_00550"
     CDS             115862..116644
                     /locus_tag="PKI50_00550"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT02238.1"
                     /translation="MNDINSKYAKEFYFKGNNTGVLLIHGFTGTPYEMRFLGEFLRDK
                     GCTVRGILLKGHGTTLKDMRRCSYRDWIHGAVEGYRSLKQECDEVFAVGFSMGGLLSL
                     YLARNYDIRGVVTLSAPIRVYSRAAALAFIERNLRNHMTGKTENREINVIGYERAPLI
                     SVHHLFKLIRYVKANLKYVKKPVLIMQSYGDRAVSPKSANIIYGSIGSKDKSIIYLHK
                     SGHVITCDKEKEQVFKEVYSFIKNKSTFIREADRKEERCSSV"
     gene            116637..>118049
                     /locus_tag="PKI50_00555"
     CDS             116637..>118049
                     /locus_tag="PKI50_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNT02239.1"
                     /translation="MFNTEKEINQEIKSKLLAFMREDAYKPLSHDELIQAFQDDSLQV
                     PELDEVLNSLLEEGSIIITRKNKYGLTEKMNMVVGRIQGNKKGFGFLIPDNKDLRDLF
                     IPAENLNGAMNDDRVIVRLTAGESGTGKREGEVVRILKRANAKIVGTFENGNGYGFVV
                     PDDQRIYQDVFVSKSDFNGAKDGYKVVVEITRWPEARRNPEGIVVEVLGHKDDVGTDI
                     ISIIRGHNLPEEFPIEAENQAGKIPELVSDADIAKRRDLRDKTIVTIDGEDAKDLDDA
                     VSLEILPDGNYMLGVHIADVSQYVFENSHLDKEALKRGTSVYLIDRVVPMLPKRLSNG
                     ICSLNPQIERLTLSCDMVIDKKGKVKEYEIYESVIKTRERMTYENVNKILAGSDPMLT
                     ERYDYLADTFRQMEELMLILNKKRRLRGSVDFEFDETKIILDEKGRPIEIKPYERGIS
                     ERIIEEFMLVCNETVAEHMYW"
BASE COUNT        37179 a        20530 c        29496 g        30844 t
ORIGIN      
        1 ttcggattcg gtggtaatga ttattaaact gtaacgacag ggggacgcgg ggacggggac
       61 acggggacgt ttcccctgtc ccactttcag cgggacacgc gaaacgtccc cgtgtccccg
      121 tccccgtgtc ccgtccgtgt cccggatttt tttgttgtca ccaaattaat gcatgtacat
      181 attatggaat tgggaaaggg tgtcaaccat acagtcctga cccagatgcc ataactcacc
      241 tcaagcatgg aagggggctt taatgcccgc tttcatgctt taagcgggaa acttgtcacc
      301 aaatatacgc atgtacataa aatagaaatg aaagggggtg acatcatggc aaacggatta
      361 ggctcaaccg gatgcggatg cggtgctcct gcgtcagtaa gtccgatctt agataagtgc
      421 aagggtaacg gattcctctt gctgcttctg ttgtttgtgc tcttagcatc atgtacaacc
      481 ttgggcaggg agaaaaactt cttcttcata ataatacttg caataatcgc tttcggcggt
      541 tttggaagaa ctttcggttt taacaatggc acagctatat aaccagattt gttcttgaaa
      601 ggaggtgtaa atatggttga cggagtagca ggcaatgctt ttggaggcgg aaactttgat
      661 cttcttcagg atagcattgg agagactgtt acaatattta ctacaagcgg aggcctgtca
      721 ggatgcggct ttacgggagt agtactggct gtaaatccat gttttgtcag gcttattacc
      781 catataggag cggctccgaa ctgtgcattg ggcagctgct gcggagtacc cgacggaggt
      841 tacggcagct atggcaatgt gagaacggta ggatcggtaa cggatattcc tcttgacagg
      901 attgccgcag ttgtacgtaa tgccatttag gaagctgtat cgaagggcag gggaagtcac
      961 ctgtccttcc tattactctg aactttttgt tcatacaggc tgtcaccaaa taaatgcatg
     1021 tacatattat ggaattggga aagtcagaaa cccaattatg cgaaaggggg tgtaacaatg
     1081 gcaaatggaa ttggttatgg cggaaatttt aacggcaatt tctttgactt actctcagac
     1141 cttataggag agactgtaac aatattcact acaagcggag gccagtcagg ctgcggtttt
     1201 acaggtgtaa tacttgctgt aaaccaatgc ttcgtcaggc ttgtaaccca catagggccg
     1261 gctccgggat gtgcccttgg caatgcatgt catagtccat gctgtcctac aatcggctgc
     1321 agcggaggct ttagcaatgt gcgttcggta ggctctgtaa cggatatacc tcttgacagg
     1381 attgttgcgg tagtaagcaa tgctgtataa atttcttatg cagtgaataa atgaaggaca
     1441 gggggggcgc cctgtccttc cgctgttcct atgtgttatc ggttattatc ttgcagttgc
     1501 ctatgaatat ggcaccttca tcgataatga tgttttttac tgtgatatcg cctacaagct
     1561 ttgcagtatg ggtaatatga agttgagagg ttgcaattac gttgccttct atgactccgg
     1621 caacaaatgc attttcggtc tttacgttgc ctttgacagc accttcttct ccgacaatca
     1681 ggttgccgga tatattaagg cctccaagca ctttgccgtc cagccttaca gtaccgtctg
     1741 cgtttacagt accttcaaat attgttccgc ggcctataat tgtatctact ctttcactgt
     1801 ttattgattt tttgcctgag ccaaacattt tgctacctcc aatctccata atccaatgct
     1861 tctgatcagt taaaatagtt tgcgggattg acgttttttc cattcagctt tacttcatag
     1921 tgaagatggg ttccggtgct tctgcctgta ttgcctacct ttgcaatggc ttgtcccttc
     1981 ttgaccttgt ctccctgctt gaccaacagc tttgaattgt gggcatacag ggtagtgagg
     2041 ccatatccgt gggatattat caccattctt ccatagccgg cattccagcc tgcgaaggat
     2101 actataccat caccggttgc tgaaacagtc tgtccgtagg aggccgcaaa gtcaattccc
     2161 ttgtggaatt cgctgcccct gctggtaaag gggtttttcc tatagccaaa gcttgcagtt
     2221 attgaggcgg ccacaggttt gacggagggc ttggccatta aatatgcaag ctgttttttt
     2281 atcggatcta tggaatcagc tattgtcttt ttttgaattg caattttatc ggttaaagat
     2341 gcaaagtcag cctcaatgct tgctagtttc aaggacatat cttggtctga tttttctgtt
     2401 aacaagctta cgggagaact gatttccgga gaaacgagtt tgatctcgat gccttcctca
     2461 ggggagggat taatacccac cgcttttttg acctcatcca gcattctgga attttcggcc
     2521 aattgctttt gtacttcttc ggaacttctc tgcagattcc caatttcaat cttctgggaa
     2581 gcgttgatgg ttgtaagctt tgatatttct tttctactgg catcatattg aagcttgagt
     2641 gcggataatg cataaaaact tattccggac gttataataa gcagtgcaat ggatatggaa
     2701 acaaatccgg ggaaccatgc cggcgtcctg aaggatttaa cattggattg gctctcggga
     2761 ataaacatga aggtagtata gtttattttg tttttcacca gtgctttttt caaatgaatc
     2821 atcctttgac attataatgc tttaaaattc atatagaaac aaaaatttca agctgcatca
     2881 ttaaatatac aggttgaaat cagcaatgga gtcaaaatta tctatttttt aatatgctca
     2941 aaatccattt tagactaaat ttgcccgaat gtacagtccc aaaattatca aagttttgga
     3001 actattgcat taaatgatgt atttcagggc actagaagat atcgaataaa aagtaaatat
     3061 ttagaggaga atatatttat gaagaaattt atggaggtat tgatatgaac aaggtaggaa
     3121 tagaaaagcc cttgagcaac gtcggaagat atctggaaag cagggactgc gctgtggaag
     3181 tgctggagga cgataccaag gaatcccccg ggaacctgaa caaattcgat gccattgtcg
     3241 taacaggtgc ggattccaat ttcatgggca tggaagatgt gatgacaact gcaaaggtaa
     3301 ttaatgccga gggcaaaacc gaggacgaaa tctacagcga gatattgaga gcagcggatt
     3361 tgaaaaaaag ataaaaacga tgtagcctat ttattacagc aatagtacaa taaaggtatg
     3421 caccaatata cgatttacat ggttggctgc atactttttt cattgctgaa acaattcatg
     3481 ggggcagggg tattatgata catgtgggaa cttcgggata cagctacaag gattggatag
     3541 ggacatttta tccggccggg gccaaggccg gggatttatt gggtatttat tcgcgctctt
     3601 ttgattttac ggagattaat tccacttatt acaagatacc caattccttt atgttctata
     3661 atatgcagaa gaagacggga gacaacttta ttttcaccgt taagctccat cagtccatga
     3721 cccacggaag agatgcggga gacaaggatt acgaggaatt tgccggggca gtggatgtgc
     3781 tgaagcaggc cgggaagctg ggatgccttg tggcgcagtt cccttattct ttccatttta
     3841 acagggagaa tatggactat atacaaggac tgagagaaag atttgctgat tttgatttaa
     3901 tggtggaatt caggaataac aggtgggtta acgacgaaac catggagttt ctgcgggcaa
     3961 accagttggg atatatctgc gtggatgagc cggctgtgtc ggggcttttg gatcccaggg
     4021 caatgaggac ttccgaaatc tcatatatac ggtttcacgg aaggaacgat gcgaaatggt
     4081 gggatcataa agaagcatat gaaaggtatg attatttgta taaggaaagg gagcttgagc
     4141 agtggaagga taagatattg gagcttgaca aacaatctca gaattgtttt gtcagcttca
     4201 acaatcattt cagggcacag gctgtaataa acggaattat gcttaaaaag cttttgggaa
     4261 tgatggaatg atattttgac tataggaggg cttgattatg aggacattta taggggcgga
     4321 cttcagcagg gatataaagg acagcattgc gggagtacag catatagtac gggaaaactc
     4381 ggaaagagga agattcaagt atgtaggcaa ctttcatctg acacttaagt tccttggcga
     4441 aattgagcag gccaaagcac cggacatagg gagaacgctt aaggaaatag ccttaaagca
     4501 caggagaatc cgtctgaaca tagataaaat agggtacttt gacggaaaag gaagcatcca
     4561 tgctctttgg ctgggttttt caggagaact ggataagctc tcctcattgt attcggacat
     4621 tgatgagggg atgtacaagc tgggcataaa aaaggagata aaagcctaca ccccgcatat
     4681 aacaatagcc caggatctgg tactcaaaat accttttgaa aagctggagg aaaagttgaa
     4741 ttttcaaaac ttcagaaccg ttgaaatcag ggagatatcc ttgataaaaa gtgaggaagc
     4801 gggaggaaag aggatttata cgccgatttc gacatttcag ttaaaataga ttatgagggg
     4861 caggtaacac ggaattcacg gaaatccgca gaagacacgg cgggataaat attctgttac
     4921 ataaataata ctcgataaca ttgtaagtat cgatatttac gaatgcagct ataaaaatat
     4981 atgaaagtta accgtgcaat cgcgcagcga ttccatgaaa catccgtgtg ttcagtgtta
     5041 tccgtatcca aatgtacaag ggcgtgattt cttgattagc agggatgatt ttcttcctat
     5101 ttccaaggaa gatatgcaga ggaaaaaaat agagcagttg gatttcatat tcgtaagcgg
     5161 ggatgcttat gtggaccatc ccagcttcgg cagtgccgta ataaccaggc ttctggaaag
     5221 cagaggctac aaggtgggca taatcccgca gcctgattgg cggaatgcag acgccttcaa
     5281 ggctctggga agaccgaggc ttgcatttct tgtgtcctcg ggaaatatgg actcaatggt
     5341 caatcactat actgccggta aaaaacccag aagagaagac ttgtattctc cggccggtgt
     5401 acggggtaaa aggccggaca gggcagttac cgtatattgc aacaggatta gggaggcctt
     5461 taaggatgtg cccgtaataa tcgggggtat tgaagccagc cttagaagat ttgcccatta
     5521 tgatttttgg gatgatcagg taagaaggtc gatattgttc gacagcaggg cggatcttct
     5581 gatatacgga atgggtgaga agcccgtgat acaagtggcg gaagcacttg aatcaggtat
     5641 ggaaattaaa tatataacct atataaacgg tactgcatat atagccccgg agcttgggaa
     5701 tattccggat gcgactgtga taccaagctt cgatgaggtt agggacagca aaaggaaata
     5761 tgcccgggcc tttatgcttc aatacaggga acaggactat catacgggcc gcgtacttat
     5821 acagcctcat ggggaaaggt tcctggttca gaatcctccc gccgcacccc tgacggaaac
     5881 agagcttgac gatgtgtaca gtctgccttt taccaggcag tatcatccgg tttataagaa
     5941 tcagggaggt atacctgccc ttaaggaagt taaattcagc cttacttccc agcggggatg
     6001 cttcggaggc tgttccttct gcgcattgaa cttccatcag ggcaggattg tgcaaagcag
     6061 aagccatgaa tcaataatcg aagaggcgga gctgctcctt aaagaccctg aattcaaggg
     6121 ctacatccat gatgtgggag ggcccacggc caatttcagg cacgcagcct gcgataagca
     6181 gcttaatgcc ggagtctgca agaacagaca gtgcctttat ccgactccct gcaaaaacct
     6241 caaggtggat cacaaggatt atgtcgcact gcttaaaaag ctcagatccc ttcccggggt
     6301 aaaaaaggta ttcgtccgct ccggactgag atatgactat attattgccg acaagaagga
     6361 tgaattcctg agggagcttt gtgaaaacca tataagcggg cagctgaagg tggcacccga
     6421 gcacgtttcg gacaaggttt taaggtatat gggcaagccg tccaaagaag tatacggcag
     6481 gttcgtaaaa aaatactttg atatgaacaa aaggctgaag aaggaacaat acctggtgcc
     6541 ttaccttatg tcgggacatc cgggttcgga cctttccgct gctgtggagc ttgccgagta
     6601 tgtaagggat atgggctata accccgagca gatacaggaa ttctatccga ctccggggac
     6661 actgtccgcc tgcatgtatt ataccgggct tgatcccagg acgatgaagc ctgtatatgt
     6721 acccagggat cccgaggaaa aggcgatgca gagggcattg atacaataca ggaatcccga
     6781 aaactacggg ctggtatata aggcgctgaa atcagccggc agagaagatc tgataggttt
     6841 tggggagaag tgccttataa agcctccccg gaatccggag aaaagaagga atccaagaag
     6901 ggaaaatcga aaaagacgct gagaaaagtt aaaagggagt gattatatga aagcaatatt
     6961 ggaaagaaga agcataagaa agtataccgg cgagccggta tcggatgccg atctgaaaga
     7021 cctcctgaag gcggcaatgg cggcgccctc tgcggggaac gggcagccat gggaatttat
     7081 cattgtcaat gacaggcagc tgcttacgga gattaccaag gttcatccct attcggcaat
     7141 gctgctgcaa gcacccttgg ccataatcgt atgcggtaaa ctggataacg aaagatataa
     7201 gggctattgg gcgcaggatt gttccgcggc aactgagaac atactgattg cagcgcagag
     7261 caaaggcctt gggacggtat ggctgggagt atacccggtg gaggaaaggg ttaatggcat
     7321 aagaaaaatc ttcaagattc ccgagaatat gataccgctg aatgtaattg cagtagggca
     7381 ccctgcggaa aaaaagaagc cttctgatcg ttatgatgaa aataggattc atttcaacaa
     7441 atgggaataa ttatctttta ttttactttg acgatgtaat tctgataaaa tgtatttgac
     7501 agataataat gataaaggag tgtattctga gatggggcat ttgtcaattg acggtgagca
     7561 tttgactata aaggatgttg tagatgttgc gagaaacagc cggagggtcg ggttgtcgga
     7621 acaggcagtg gaacgaatcc ggaaatcaag agattatgtg gacagcctgg tggataagga
     7681 agcggtggta tacggaatta ccacaggctt tgggaaattc agcgatgtat tcatatcaaa
     7741 ggaagatacc agagcacttc agaggaatct tatcgtaagc catgcctgtt ccctggggga
     7801 cccgcttacc gaagagactg taagggcaat aatgctctta agggctaacg cactggcaaa
     7861 gggtttttcc ggtataaggc tggagacggt aaatacactg gttgagatgc ttaacaaggg
     7921 agtgcatccg gtgatccccg aaaaaggttc cctgggagcc agcggtgatc ttgcacctct
     7981 tgccaatatg gttcttgtaa tgctgggaga gggagaggca tactataagg gagtaaggat
     8041 gacaggtaag gaagctatgg aaaaggcagg aattaaaacg gttgagctta cttccaagga
     8101 aggccttgca cttataaacg gcactccggt aatgacaggc ataggggccc ttgccgtata
     8161 tgatgccgaa aaccttttga aaaacgctga tataatagcg gctatgactc ttgaagcact
     8221 taagggaata acaacagctt ttgacaggaa ggtacatgaa gtaagaggcc agaagggtca
     8281 gatgacagct gcaagaaata tgctgaggat agtatccgga agcgagctta caacaaccca
     8341 gggacagata agggtgcagg atgcgtatac attaaggtgc ataccccaga tacacggtgc
     8401 cagcagagac gctgtgcaat atgtaaagga aaaggtatcc atagagctta atgcggcaac
     8461 ggacaacccc cttgtatttg ccgatgatga ccaggtaata tccggcggga acttccatgg
     8521 acagcctata gctcttgcca tggatttcct tggtatagca gtatccgaac tggcaaatgt
     8581 ttcggagaga cggcttgaaa ggctggtaaa tcctcagctt aatgatctcc ctgcttttct
     8641 gacaagaaag ggagggctga attcaggatt tatgataacc cagtatgcgg ctgcttccat
     8701 tgtgtcggaa aataagattc tggcccatcc tgccagtgtg gattcaatac cttcttccgc
     8761 aaaccaggaa gatcatgtga gtatgggaac taccgccgca agaaaggcca gggttatatt
     8821 ggacaacgtt cagaaggtgc tgggaattga agcatttgca gcttcccagg ccataagctt
     8881 caggggggat gtaaggctcg gaaaaggtac ctcggaagct tacaagctta taagaaaaga
     8941 gattgcgcct gttgaagagg acaggataat gtataccgac atgaataagc tggatgagat
     9001 ggttaagtcc gaagctttga taagagcagt ggaaagcgca gttggagaat tggagtgata
     9061 tgaatgaaag ctgacttgat aataaatgct tcaacaatag tctgctgtac cgaaggagaa
     9121 ggtgcgggag tatttgagaa cggatatgtg gctgtaaaag acggaaaaat aatatctctg
     9181 ggagagggaa atgcccccgg ggatatcaag ggctcaggaa caaggattat tgatgcagcc
     9241 ggtaagacag tgactccggg gcttatagac gcgcataccc atcttgttca cggaggctca
     9301 agggaaaagg agcttcctct gaagcttaaa ggagtgccgt atctggaaat cctgaaaatg
     9361 ggcggaggga tacttagcac ggttagaaac acacggaaag cttcaaagga agagcttaag
     9421 gcaaaatccg gaaagagcct ggaccagatg ctgcttcacg gtactactac cgtagaagcc
     9481 aagagcggtt acggccttga ttttgatacc gagatcaagt gccttgaggt ggctgccgag
     9541 cttaatgact cccatcccgt agacatagtg tcaacatata tgggagccca tgccgtaccg
     9601 gaggaattca agggaaacac tcaaggctat atagatttca tggccgataa ggtcatgcct
     9661 tatgtcaggg aaaacaatct tgccgaattt atggatgtat tctgtgagga gggggttttt
     9721 tctcccgggg agtccagggc cataatgcag aagggcaaag aacaaggctt caagcttaaa
     9781 atccatgcag atgagatagt ccctctgcag ggggcggagc ttgcagggga attgggcgcc
     9841 gtttctgcag agcatctgct ggctgcaacg gaggccggaa tagccgcaat ggcggaagcg
     9901 ggagttacgg cggtgctgct tccgggaact tccttttatt taatgcttgg caagtatgcc
     9961 caggcgcgaa agatgatgga caggggtgtc agggtggcac tggcaaccga ttacaatccg
    10021 gggacatgcc ctaccgagaa tcttcagtcc ataatgacct ttgcatgctt tggaatgaaa
    10081 atgactcccg aggagataat aaaaggaatg actctgaatg cagcttatgc cttagacaga
    10141 gcaggagaaa ccggaagcct cgaagtgggc aaaaaagctg atattgtagt atttgatgct
    10201 ccaaatcttg aatatatagt ataccatttc ggcataaacc atgtggatac cgttatcaag
    10261 gacggaaaaa cagtcgtaga gaacggaaga attttgaagt agatatgggg aggaatatat
    10321 gagtctttta atggatatga ctttaaggga ttacagtgat gtgctggcaa gcagcgaacc
    10381 tgccccggga ggaggaagca cggctgcgct gtcaggggtg ctgggggctg ccctcactat
    10441 gatggtagtg aatctttcag tgggcaaaaa aaattatgaa aacctgaatg aaaaaataaa
    10501 agcgaagttc atggaggaag ccgaaaatgt aaaggcgctt caaaaggagc tttccggttt
    10561 ggttgacgag gatacaaaag ccttcaacaa attcatggag gcggtaaagc ttccaaagga
    10621 taccgaagaa caaaatcagc tgcgtggaaa gaaaatgcag gaagcaagcc tttatgcact
    10681 tcaggttccc ataaagaccg cagaatgctg tttccggctg cttgaaaatc aagggaatat
    10741 tgccaggtac ggcaacagga acgccgtgtc cgatgtggga gtaggcgctt tattgacact
    10801 ttcagggctg gaaggggcga tattgaatgt gaatatcaat ctttccggaa ttgaggatga
    10861 agcagtcagg tcagaggcct atgataaatg caggaggctt tctgccgaag ggcgcaagct
    10921 ccatagtgag atacttgata tagtaaattt aaggatagga tagatataat atgataagca
    10981 gagaaatgat agacaggata aatcatctgt caaaaaagtc caaggaggct ggcttaaccg
    11041 aagaagaacg gcaggagcag caggaactca gaaggaagta tattgaatat ataaaaggac
    11101 aggtaaggca tgggctggat tcaatcaaat ttgccgagga caggcatgac tgcggacatg
    11161 aacatcatga ccattgcgga tgtaaatgta aaaaacagta gaattgtgca ataatggctt
    11221 tagtgatata attagctttg ggagggattt ttatggctga attgcaattt gttgttttta
    11281 agctgggcaa ggaagaatat ggcgtgaaca taatgcaagt acaggaaata ggccattatt
    11341 cagaaccggt caaggtgccg aatactcctg aattcgttga aggaataatg aatctgaggg
    11401 ataatgttat tccggtaatt agcctaagaa aaagattcaa tatcccggaa gagaatttga
    11461 acggaccaga gcgggattta aatgaaaaca gaaggactat tattatcaat ttaggggaca
    11521 ggcagatagc ctttattgtc gatgatgcat cggaagtgct gacattagac gaggccggca
    11581 tacaggaaac tccggatata atcgccgggg ttgacaggaa atacatatcc ggtataggga
    11641 aaaaggggaa taggctgatt ataatgcttg atttgaattt tttgtttgat gagcaggaac
    11701 aggcacagct tgctgccatt taaacacttt tttatagtgc ggccttttac cgcacttttc
    11761 taaaatacac gaaaaaataa atttttttat aaatgattcc tgattattca acgataatat
    11821 tgatatgagg tatattttgc agtattgatt atttggaaga aagcagaggg tgcttattgt
    11881 ggacaggtta aatgaaatca taaaaaatac ggtagatatt attgaaaaga gcagaagtga
    11941 attattcagc atagctgaaa gctcccgcac cgaatgtcaa ttggtagagg aagaactgga
    12001 aaaaatcagg ataattgcat cagaccttat caatcaggtg gacatgctgg taaagcttga
    12061 caaggaatgc agatatcagc ttatgatagt gagtaagaat attaaggatt acaaggaaga
    12121 ggatgtaaaa agagcctacg ataaggcaaa ggatcttcag gtagagttga tgacgaaaaa
    12181 agcggaggaa cagctgctta tacaacggag aagcgagctg gaaagaaggc ttaagatagc
    12241 aagagacact gcaaacaaag ctgaatctct tgtcaacaag gttggagtgg cgatgggcta
    12301 tttgaccgga gagctgcagg acctttcgat acagatggag gatataaagc agaagcaata
    12361 tctgggtata aagataatca aagcacagga agaagaaaga aagagagttg caagggagat
    12421 acatgacggc cctgcgcagc tcatggccaa tctggttatc aaaaccgaat tatgtgaaaa
    12481 gcttatagat atggataagg agaaagcaaa ggaggaactt ggcagtctca gaggtttcct
    12541 tcgttccagc ctgagtgacg tgagaaagat catatacgat ctgcggccta tgtctctgga
    12601 cgatttggga ctggttccca cccttcaaag atatatttca aactacatcg aacaaaacaa
    12661 catatcggtg aatttttcgg ttttgggaga tacgaagaca ttaaagccgg ttgttgagct
    12721 tgctgcgttc agaatcgtgc aggaagcatt gaccaatata aaaaggcatt ccggagctga
    12781 taatgcggtt gtcagagttg agtttaccaa tgaccaggtt aaccttctca tcagtgatga
    12841 cgggaagggc tttgacaaga acaagctgtg gaaggcaaaa gaagaagatg caggttatgg
    12901 cttgctcaat atgaaggaaa gagtcgaact gttgaacggg aagtttgacg taaagacagc
    12961 ccgtaataaa ggcactaaag tatttgcaag cctatctttg aatatagcag aggagggata
    13021 atgtgagtaa tatacggata ttaattgcag atgatcattc catcgtcagg gaaggactca
    13081 aacagctcct ggagctggag gaagattttg aagttgtggg acaggcttct aatggagttg
    13141 aaaccataga gaaagtcgag gagcttaagc cggatgtgct tcttctggat ataaacatgc
    13201 ctataatgaa cggtataaaa gctcttaaga agcttaagga ggacggtatt gacacaagag
    13261 tcgtgatact tacgatacat gaggacaggg aatacctttt agagacaatg caaatcggcg
    13321 cttcgggtta tatactcaag gattccgatt cggcaagttt cttcaaggcc atcagggatg
    13381 catatatagg ggagtcatat atacagccga aactggccgc tgatttgata aaggaattca
    13441 acaagccaag aggagccagg gttaagaatg aaaatgagct tacccagagg gaatatgagg
    13501 taatcgtgct tattgcagac ggtttgaaca ataaggatat cgcagacagg ctttttatca
    13561 gtgaaaagac agtaaaaaat catgtttcaa atatattcag aaaaataaac gtcagtgaca
    13621 ggacacaagc ggcaatatac gcatataaaa acaatatcaa gaagtaaagt acactctgtt
    13681 acacagttgt aatatttgga agcattgcaa aattatcaat ttggtggtat attttattag
    13741 taataaaaaa ttatgccggt atgagcatga tatgttatca agaaaaggag gtcgtgaata
    13801 tgaacagaag cgtaataatg atgattagta ctgcagtaaa agaagcagcc tgttttgtat
    13861 atggcatcag tgtgccccaa attatatatc acgacctggg atttgaataa tagcgatagt
    13921 tattttggtc acgggccgta aaacccgtga ctttgtattt atatgaagac gctttaaaag
    13981 tcatgggcag agacctgtgg cttttttatt tttgaaagga ggggttataa atgacaatag
    14041 caagagtgtg tggttctatt gaaaatacta cagtaaggaa gtttgacagg ggaggtaaaa
    14101 gcatgaatat gatcattaag ttattgacag ggctaaagtg caaggacagg gaaaacagag
    14161 atcttaatga ctgcgggtat acattgtatc agtcattgga atataatctg aagtatttaa
    14221 aaggtgatga tttcggatat agagaccctc ggacacaatc aacagatgat ctaatggcaa
    14281 ggaaaacatt ttaagatatc cggaatatta ttgccaaatg cctatacaaa aaatattagt
    14341 cgcgaatgaa tgaaaattta aatttgttcg cgacttatta ttttgataat ttttcatgtt
    14401 atgctaaact atatataata tcaccggata caaggctgcg aaatgataac aggggagtga
    14461 atcatttgga taagcagaag ctgtacactc ttttacaaaa gccggaaggt accaagctgg
    14521 attttaaagc cgtactttca ttaaaaactg aaagtgagaa aaaggagctg gccaaggatg
    14581 ttgcagcaat agctaattca aaaggcggca ggggctatat aatttacggg atacaggacg
    14641 agacaaaagc tatattggga attgagggaa agcgttatac agaagagcag atacagcaga
    14701 taataagcca gaggtgtgac ccgccggtat ccgtgaagct tgagataatc ccagttgatg
    14761 ataagcagat tgctgtcctg acaatataca gaagcagcca gaaaccgcat cagataagac
    14821 agacaggtgt attttacata agaagaggtt ctactacgga tatagcaagg cgcgaagaga
    14881 tagccagcat gctccaggaa tcggggatac tgcagtatga aagaattgtg ctgaacaggg
    14941 tggaattgaa ggaactgaac gaggatataa taagtgatta tatttccaaa acagggttta
    15001 ccagcagcag cgaaaactat tatacacttc ttgagggaat aggcataata ggaagagatg
    15061 aggactccaa cgggtatcac cctacggtgg gagggctgct ggttttcggt tataatcccc
    15121 aattgcacct tcctcatgcc ggaataaggc ttattgacaa atctgcggct gataaggtaa
    15181 tatatttttc cgggccgatt attagaatgc tggatgaaat tgaagactac ctgagattta
    15241 aagcagagaa aataaatggg aaatatccag ttgctgctgt tgttgaagct gtggccaatg
    15301 ctgccgtcca tagagattat ttttctccgg gaagagagat agtcatatcg atagataata
    15361 aaaagataga aataagcaat cccggcgcaa attgcagcga tgacgacata caggggctta
    15421 tggaggagta caatccctgc agaagaaatc agtggctgta tcaaaggctg ctgatacttg
    15481 acaataaggg gagattcctt aatatagggg caggcatgaa aaaaatacat gaagccttca
    15541 agcagcatgg tggtgccagg ttcataaata atgagaagag gaatcttttc aaagtagtgc
    15601 ttccgggttt tatgaagtat taatctatat tttacattaa agttggtgat gaaagtatga
    15661 acaacaaggt ggtaaccttt gaaagagggg cagattttta tttcgacctg tcatacaagt
    15721 atatacaaaa gggcaggctg aaaactgcac tgagatatat tgaaaaagct gtaagcctca
    15781 agcctcatga cagcttcctg cagttcaatt atgcgggact gctggccgaa caggggcata
    15841 tcgaccgttc cacagaggtt ttgcttaata tagttaaaaa catcgaccct aattatctgg
    15901 agtgctattt tggcttgggc tgcaactatc tgcagctgca gaagataaaa aagtcctcag
    15961 agtatttcag catttatctc gaaaaggatc ccggcggcga gttcgcagag gaggcggaag
    16021 agttgctgga gatgcttaca atgataaagg atgccaataa caacatggat gatgaggaac
    16081 ttgaaaaaat atataaaatt gaagaagagg caattgatca cctggagaag aaggaataca
    16141 agcaggccac cgaaaaattt gaaatagttg tacagatgct tcccaacgcc gtaccggcaa
    16201 ggaataatct ctctttggca tattattacc tgggatttat cgacaaagca atagagcttg
    16261 ccctggaggt gcgtacctat gaagaggcca atgtgcatgc aaactgtaat cttgccatat
    16321 tctacaataa gaagggcgcg gccagcctgg tcaagaagca gttggggata atcagcagga
    16381 tccgggctga cagtgccgat tacctgtata aaataggtga tacctatgga agcttgggaa
    16441 agcacgccga tgcatacaaa accttcaaga agcttttaat tattgattct aaaaatccta
    16501 tttatgtaca ccttgcagca gttgcggctt tcaacagcag aaagtacagg gaaagcatca
    16561 gactatggga gaagctgggc cagattgaca aagaaaatat gctctcggat tattatatag
    16621 aagctgccaa aaatcagttg gatgctgaag atgaaagaac atattttcct tatttatatc
    16681 agcttccaaa ggaagaaatc aaaaacaggc ttgattttat atacaagttt attgaaatgc
    16741 cggggcctaa gtgcagagaa cagttggcag acaatgagca tatgcttggc atgctttact
    16801 ttggaattaa ttttgataaa tattttttga ggaaactaat attcaataag ataaagaatg
    16861 acaggatgaa tgaggcggaa agctttatca gaagattcat gctgagggat gatgtagaga
    16921 attatataaa gatggaagca gtatttctcc tgaatttcat aggtgcaaaa gaaccgtata
    16981 ctgtgaactt tgacggggaa aagagaaatg ttacgataga ttctctggat ttccccgagt
    17041 ctgaatggga aaagccatgg ataaaggtga agcgtaaagc catatcaatg atgcgaaata
    17101 aatataagag gccttataag aagattgttg aagacatctg gtatgaattc ataaagtcga
    17161 cattcccgga ggtaccggaa atagatgatg taaacatatg ggcagccgca ttggagcata
    17221 cttactgccg tttgtgcaat gctgaagcat cggaacataa gctggcggag aattacaata
    17281 tatcatcagt ctcactgctt gaaaaggcag aaataataaa tgaagcaata aaaaataaat
    17341 ttcagtgaga cagggagaaa cgtccccgtg tccctccctg tctcacctgc cctatgtccg
    17401 ttacttcttt tttcttactg aaaaaccgaa atggcccaaa gacttcgcga gagccctgta
    17461 ttcgccgtgg ttatagcata tcaggcctgc cttattcagg tgcagctttc cggacttgtc
    17521 taaaagcttt tcctctgcat caacgcataa tatctctgcg ataaacatat catgggagcc
    17581 cagggatatt atattattaa cccggcattc caggcttaca ggacattctc cgatggaagg
    17641 ggcatcaacc agatttgatt tttgctgggt gaggcctaaa tgctcaaatt tattaatatc
    17701 cctgcctgat tttaccccgc agaagtcaag ggcatatgcc aggctttcgg tggggagatt
    17761 tactacgaag ctgccggtat ccttaattat gccgtgggag aatctttcag gccttataga
    17821 gacataaagc attgccggtt ctgaacagac tatacccgtc catgcaaccg ttatgatgtt
    17881 gctcacacct tctctgcttc tgcaggatac cattacggcc ggcacgggat aaagcatagt
    17941 accgggtttc cacatctgct tagccattgc ttcctcctta taattcatca ttcttaatat
    18001 ttgtttatta ttatttatta tatcataaat ccgcaaggat ttgagacatg gggacgtttc
    18061 gcgggacatg gggacgtttc ccctgtccca cttccagagg gacacgcgaa acgtccccat
    18121 gtcccgtctg acgtccccat gtctcattac ccgctgtcac caaaatcccg gtttgtacat
    18181 agtatgaaag tgggacgtta atcaaagtaa ccaatgatga acaaaggaat attatgataa
    18241 taaatcaata gagattggag gtcacaacat ggcacacggc aaggataaca atgtgtctga
    18301 cagcaggatg aatgaactga aaaatatggt ttccgaaaag ctgggaggca tggatattga
    18361 caacgtatcc ccggaccagg ttaaaaatat gctggtaaat gaaataagcc ggaacagtaa
    18421 tatagaccct gccataaaag ataaaatcaa caagggggat gtagacggat taaaggagga
    18481 tatcatcaaa tatctttcca ataacaggtc tgcagacggc tcaagtgatc agctcataaa
    18541 catgcttaaa gacaatgata tggaaggtct gaaaaaacag cttatgggca tgctcctttc
    18601 ggggctgggc cctcaaaaaa aaaatgagat aaaagagccg gaagtttatg aaagccaggc
    18661 agacttaaaa gacagcctgg gggattttga tgaaagggca cttttgggct tgctttttga
    18721 taagatgttt gcaggggtaa aagatgatag caggatttca cttttatact cgctaaggcc
    18781 ttttgtatca gaaaaaaggc aaaggggcat agatgattgc atcaaaatta tgaacctggt
    18841 tgcatttttt gaaaatttta tgagcaaggc agggaaataa aatgagtacc gagcatttaa
    18901 ttttaagtat tcttaatcgg ctgagcccgg aggatttttt aatccttgcc attgctttta
    18961 tcttgtatat tgaaaataag tgtgacaaaa cctttctgat tattacgctt ctggtgtttt
    19021 tgatggattt gcccgaatag cgatggcaaa tatggtgcat tactacctta agggttgtaa
    19081 caatgcctcc acggcagata aagtgcttta tcaggagcct ttctcattat agggcagata
    19141 tagttggaaa attggaagag gaggtatata tcagtcaata tttaatttgt acatgcatat
    19201 gaataaatag tataatatta acaaatgatt tactaggggg aattatttta atggacggtg
    19261 acttatggac gcaaatattg cttataatat tgcttatagg tttaaatgcg ttttttgctg
    19321 cttccgaaat ggctataatt tcagtaaggc aatccagaat caagcctctt gtggatgaag
    19381 gaaacaaagc tgcaatttta gttaacagat ttcttgaaga gccgagcaag ctgctggcca
    19441 caattcagat cggaattact cttgccggat ttcttgccag tgccattggt gctcagacgt
    19501 taacaaacgg attgaccgga ttcctcatga gcttgaatat tccgttccta tctgcttcag
    19561 ctgtcgggat ttccacattt attgtaacca gtataattgc gctgtttact ttaatactgg
    19621 gagagcttgt ccccaaacga atggcccttt cacagtcgga aaagacggca tttagggttg
    19681 ccggcacaat aaaccttttg tccaagttaa cttatcctat tgtaaaattt cttaccgtgt
    19741 ccactaattt agtagttaag ctcatggggg ggccggcgaa ggtaacggag gaacagataa
    19801 cagaagaaga aatccggtcc atgataaatg tcggggaaga gaaagggata ttccgggaag
    19861 ccgagacgga aatgattaac agtatatttg agtttgatga tacggttgca aaagaagtaa
    19921 tgactcccag aatcgatatt gttgctctta actccgaagc cactcgtgaa gagatacttg
    19981 atgtaatagt gcaggagaac ttttccagaa tccctgtata tgaagaatca attgataata
    20041 tcataggaat tctatatgta aaagatttat ttgcaatgat aagaaataat gtcgaatggg
    20101 aggtatccct taaagaaata ataagagaag cacactttgt acccgagtac aagaagattg
    20161 acgagctttt caaggaaatg cagaaatcca aaacccacat tgccgtagtc atagatgagt
    20221 atgggggtac ggcaggaatt attacaatag aggacttgct tgaggaaatc gtcggcaaca
    20281 tatttgatga gtacgatgat gtaattctcg attatgaaaa aattgacgac aatacatata
    20341 ttgtcagcgg aatgctgaat gtaagtgatg taaacgatat attgaataca aaactatccg
    20401 gagaggagga gttcgataca ataagcggaa tgctcctcag ccactcgggc aaaatgccgg
    20461 aggtagggta tgagttgaca atagacaata tatatttcag gattgaagaa gtagatgaca
    20521 agaggatatc aaaaataaga attgagaaga gagatataga accggataaa acagaagaat
    20581 aacagcacag tcaataggac tttcggttaa tatttcagat ataaactttt tgcccccctt
    20641 tgttaataat cgggattaat aacaatttta gggggttttt tgttgagagg acaggttttt
    20701 acaagggtgt aaaacccatc attgaaacct tgttacagca taaaaatata acttattggg
    20761 gtgaatgata tgagatttac ggatagcaga tcaaaaatat tattaagtga acgatggctg
    20821 tccgttattg cccactcatt gttttcatcc tggatgttat cctttatttt tgaaggagaa
    20881 gttttttact ctctcatggg aatctttgac tttgattccc atgctatggt tttcggagga
    20941 attgcagctg tatttttggg gctgctgcta agcgggtttt tcataaaaac aaaaagagga
    21001 gccaagagaa ctttcctgta ttcttacctc ttttgcattg tcatgtccgt gccttttttc
    21061 tttccaccct cggtattatg gactgtggga attattgcag gctcatttat gtccggaagc
    21121 tgtgtggctg catggggtta ttatttgaaa agcagcacac ccaaaatcgg gcgtataaaa
    21181 accgttgcag atatgctgat actgtcaaat attcttatga tcttactgaa tatggcagca
    21241 ctctggatat ctccacacag cggattggct ttatcaatga taatgctgat ccttgcattc
    21301 ctgtttgcac tgaaactccc cggggataat tgcatttcgt cttctgcttc ctttgagcgg
    21361 gataaaagcc ggataagcat agcgcctctg cttgtttttt tatgtatctt tattatagtt
    21421 attactatta actcgggttt gatgtatcaa gtggttaatc ctgcctttgc ccatcttaaa
    21481 tggctgacaa gctggtattg ggcaattcct tatattattg ctgtttttat aatgggtcat
    21541 ctgcctcaaa aggtgaaccg ggcttatata ctctacgttg caatcgctat gatagggatt
    21601 tcattcataa gctttatgat ccttgaccat aacgttgccg gttatattgc agttaataca
    21661 ttgatgcttg gagcctttgg agtatgtgat ttattttggt ggagtattct tggagaaatg
    21721 ctggattttc ataaaaaccc cgcagcaatt cttggaatag gcctctccgc caatgtgctc
    21781 gggatactgc tgggggggtt gatagggaat gctatagtat ctgtcggcaa acaaagcgca
    21841 gaccctgtat tgctggcgct ggccgtagtt tgtgtggcac ttgcactgct gccgtttttg
    21901 catagatatc tgtcttccct tctcagagat catacctact taagaatatt ctcggaaata
    21961 tctgctcagg aacagatcag acagcctgac tatgacgcag tgttcgagag cctgtcagag
    22021 cgggaaaatc aggttgcatc ccgtttgctt cagggaaaga cttacaggat aattgccgga
    22081 gagctttata taagtgaaaa tacagtgaaa tattatgtaa aaaatattta ttcaaagttg
    22141 aaaatacaaa gccgcggaga acttgtaaat atttttttag aaaagagagg tattgggaca
    22201 ggcaaataaa aaggatggtt ttgtgtggga actacccggt cgggtaggtc ttttttttac
    22261 cctgtgatgc tactatgaaa tgagaaagga gaatggtcaa tatttcttgc atagcagacg
    22321 gggagtagtt tactaaaaag taagaatgaa aaggaggagt tccatgaaaa aagcgaacag
    22381 aaaaaaaatt acatggggtt tggtcacaac agcgctgctg ttaaccattt cattatcagt
    22441 gaatttcctg atacctgaaa cggtatatgc cgatactacc ggaggatggc atcttgcgga
    22501 aacgaattat tatttgtcaa catcggatta cacagtcaag ggagaaatac aaaatggtat
    22561 aattgggacc tcagatgtca tatatgataa atatatggtt gaaggctctg aaggggatat
    22621 gacatttacc cactcacgca gatatgaaaa tgggaatgcc attgcccatg tgacttatca
    22681 ggttgtttgg gatacccctc cctcctatat tcctgccggt caaaaaaccg gattcaacat
    22741 tgattataag gtaatcagtt ccaagtcttg gggcggagta cagataaatg ctcatttgga
    22801 tgcggcaggc ataaagcccg ggggcgctac atcatcctat atatctttcc aattggaaga
    22861 tgggacaaag tatattaata gtgataagca aggctacttc gaaagcgaga aggtgattcc
    22921 cgagggccgc ccgggggata aaaaagctat tatcctgacc ttgggcaacg gctatggcta
    22981 tacctatatc tatgagtgga aagaaggctc tgcacctgtt gcaatcccta cagctcctgc
    23041 agcacctgta tcttcgggaa gcggcgctga accctttgaa tcgggagcga gaattatgtg
    23101 gcagcctgcc aacggtttag gatacaggct ctttagatcg gatgccccaa atgatattgg
    23161 gatatccgtt acagattttt atatcacaag cacctcctat gcggatgtga atgttgaacc
    23221 caaaaccact tactattatt cagtaaagcc ggtattggca gaagcaaggc cttttgaagg
    23281 aatcgatgaa aagcttgggg atgtgattgc aacatttgtt gtaacaacgg gggatcaggt
    23341 atataagccc ggaagctaca agcactttat tatgttaaaa cttgaagacc caaatatgag
    23401 cgttgacgga gtaagccagg aagttgatcc gggaagagga accgctcctg tagtaatagc
    23461 ggggagaacc atggtgccaa tccgtgcggt tgtagaagct ataggcggta cagtggagtg
    23521 ggatggagcc acaaagaaaa taaccctgac agcaagagga aatactgttg aaatgtggtt
    23581 gggcaaaaca gatataaaaa taaacggaga aagcaaaaaa atggatatag cacctgtttc
    23641 aaaaaatgac agaacttttg tacctgtaag atttgcggct gagaatctga attgcaaagt
    23701 ggattggata aacagtacca aggaagccgt aatagtatac gaggaataat gtggtattga
    23761 acagcatctg ctgttaatta atgctgttaa gaaaaagcag attgaagtaa gaacaaaaaa
    23821 taattaaggt ttacaaagtt ttctttgtaa accttttatt ctcaatatgg tgccgaaggt
    23881 cggactcgaa ccgacatgct ttgagggcgc atgattttga gtcatgtgcg tctgccaatt
    23941 ccgccactcc ggcatttgtc acataaaaca tgatagcata catatgttta aaaatcaata
    24001 cgaatatttg cctttgcata ctgcggaggg tttacaattt tctttaaatt tttatttgaa
    24061 aagaataaat attatgatat aatgaaccct gtgatttata ttttatgtat acttatcaat
    24121 gtatttaggg ggacaattat taatggatcc agtaacccac gcagcagtag gacttactat
    24181 cgcagtaatg tccggagaac ctttggcagc ttcaaatcct gcaatgatag cttgtgtagc
    24241 gggcgcaata attcctgacg gcgatatatt aatgcagctt aagggcgact atgcatattt
    24301 gaaaaaccat agagggatgt cccattcaat accaatgatg tttgtttatt caggcataat
    24361 aaccggatta ttgatgctgg ctttcaacgg tgcagcattt tggaatattt ttccccttgc
    24421 cttgctgggc tgtttttccc atattgccct ggatatcaca aattcatatg gagcacagat
    24481 attatggcct ttgagcaaaa agcatataac ctttgatttg ctgttggtat acgaccctat
    24541 tcttataata ttgtgcttga ttaatattct gccttacaca aggggtatta ttccgcctta
    24601 tatgacagta tttatttttg ccggctattt aatacttcgc cgcattatga aaaagctggc
    24661 acaggatatt gcttatcaga atctcggcta taaatacaaa agcgagtatt tcagaataat
    24721 gccttctatg ataggaatta taaaatggca ttttgtattg tatacaggaa gaagaaagat
    24781 aatcggggaa gttaatatat ttcccagaag gttcaggata atagacaata agaaaaacat
    24841 tgacacagat ttatacaaaa ttgccatgga tactcctgtg ggtatgtttt ttgaaaagtt
    24901 taccccggta tttcacgttg agtgtgaaaa gaaggaaggc aattatgaat ttgaatttat
    24961 agacctcaga taccacattt caaaagattt cctgcatcat gccactgctg taatcaacag
    25021 ggatcttgaa gtggtgacat ctttgtttca tccttataga aagaccagaa atgtagaagt
    25081 gtagaaatac aacatttaag cctgaagaag tcattgacct cttcaggctt tttcatttag
    25141 cgcataagaa gaataatgcg acacggggac gcggggacac ggggacgtcc ccgtgtcccc
    25201 gtcgcattat tcttctaata ttcatcagat tcgaataatt ttataaatcc aataataaat
    25261 atagattgtg tgtacttgta ttaaaggggg atttattttg aagaggtctt tttttattgc
    25321 acttattgtt attatttgtt tcagccgggt tttggcagac agcggctcag ataagtcatc
    25381 cattgagatt aatctgcctt ccggcactct gagcttgtac aaagacggca atctggccaa
    25441 ggagtatccc ttatgccttg gaaaacagtc aactcccacg ccggccgggc aatacaggat
    25501 aatatacaag gctgttaacc cctattggct gaacaagggt gacgtagtgc ctcccgggcc
    25561 tcaaaatcct ctgggcgtaa ggtggatggg aatcaccagg ggcataggca ttcatgggaa
    25621 taacaaaccg gagtctattg gaacctatgc atctgcaggg tgcataagaa tgttcaacag
    25681 ggatgtggta gaagtatata atcaggtaac ggtaaatacc cctgtaaata taaaatatga
    25741 caggctaaag ctgtttgaag acaagtactc aatgaaaaag agcataataa tatatccgga
    25801 tatttataaa aaaggtgcag aaattgccgg acagcaattg aggcaattaa gcaatacgga
    25861 tatatccgaa gaaattttga aaaaagctca ggaaattata gcaaagccca ttgccaagcc
    25921 tctggcggta tctgacggaa tcggagtgtt cctgaataac agcctgatga cctgcgatgc
    25981 tctggaggag gagggaaaaa tatatgtgaa ttacaaagct gcagaggata ttctgggatt
    26041 gactccggaa ttggccggca aatttaatat agggattaag gaactggaag gggtgatata
    26101 cataaacctc aatgatacgg cggatcgctt cggagggact gtgagctatg atgaagcagc
    26161 tgatagtgca tatatagaca tggaagttgt caaggtcaac ggggtttttg caggaataaa
    26221 ttatggggat tacgacaaat cggattattt gaccgttgaa acggttaaac accttggata
    26281 tgagtattcc gaagactctg ttgatataag ggtttttgac aaagggataa tgaagcttaa
    26341 gagaaagaat gcatgggtta ttaatctgga taatcttgcc gaagtagtgg gaggctctaa
    26401 agatacaagc tcccggtacg ggattgtgga tctcaggctt ccggcttatt taaaggttgg
    26461 caaagagtat tttaaaacgg acaatattga gggaaaacca gtgctgaatg cagaaacagc
    26521 ttacagcata tatgaaagaa gcggccgggc tgttgaaacc ttttccgctc agggtcagaa
    26581 tatcgatgaa aaaatagacc tggaaacttt tttggaagga catgactata ctgtaaatga
    26641 tttttatacg ttaattgata ttaaactaaa ggaaaattag tagtataata tacttgtcag
    26701 caagctgcaa aaaacagaaa agacgctgat taaaaatatt ttaaattttt tgccggaagt
    26761 cccgaacttt ttttcaaatt ttacgtctaa taatattaat gattatgtct atattattag
    26821 tgcctccttc ggcattaaat tattgaaaat attattcgat tgagtgatac gtggggtttt
    26881 ggcttgtcaa aatgcatatg acggatgtat ttaaatagcg agagggggaa atgctgtgag
    26941 tgacctggaa aaactgttaa taaagaagag ccagtccgga gatgttgaat cctttgaact
    27001 cctgataagc tcctatgata aaagggcata caatattgct taccgtatta tgggaaacga
    27061 agaagatgcg aaggacatgg cacaggaggc actgctcagg gtattcaaat ctttgaagga
    27121 ttttaaggga caagcggcct tttctacatg gctgtataga attgtgacca atgtttgcct
    27181 ggatgaatta aggcgcagga agaatgaaaa gtatgtatcc atggacagca caatacatac
    27241 ggaagacggt gagctgcaca tggagctgtg ttctgacaag gaaacccctg aaagtgttta
    27301 tgaaaggatt gaacagcggg aacttatcag gaatgcaata cgggaaatga atgaggatta
    27361 tagaactgta atagtattaa gggacataca gggcttcagc tatgatgaaa tatccggcat
    27421 gcttgactgc tcccttggta ctgtaaagtc aagaattaac agagcgagaa ccatgctcag
    27481 ggataagctg aaagaaagca tggaactttc ccgggaaaat atagtctaat ttggtgaagg
    27541 ggggtagaag ggctatgaag tgcgaaacaa taaagaatat gatgtcttca tatatagata
    27601 aggatttgaa taatatagaa aaggccgaat tcgaaaagca ccttgcggaa tgcgaagaat
    27661 gcagagcgga atatgagagc ctgattgaca taattgctgc atgcggcaat ctcgaagaaa
    27721 ttgaactgcc tcagaacttc agaacggaac ttcaccaaag gttgaatgag gagaaaagta
    27781 aaaggaattt cttcagcggc ataatgggaa gcaaacgcat gaagatggca gcgggacttg
    27841 ttgccgcagc actggtaatt gcgataggaa taggaggttc atccttactt tttgataata
    27901 gcatgaaaat ggcacagagc gctgattctg caccgggtta tggcatagct cccgcacctg
    27961 ctgcaatgcc tgaagcacct gcggcaccgc cggtttatta tgactcagac cttgccgaga
    28021 acaaagccaa gtcgagagaa ttcgaaacgg ctgcggcaga ggagcggggt atgggagagg
    28081 attctgtcgg catgcaattc aatgaaagcc ttatggcaaa ggaaatggcg cccagtcagg
    28141 gcctggaggg ccaggctgct gagagcgcaa gatcaggcag aatggtaata agatcgggca
    28201 acatatcagt gaatgtggaa aatgtagata aggcagccgc tgacattagg cagatgactg
    28261 aaagcagcgg aggctatgtg gagaattccc agattgataa tgtcacagtg ccgcagtttc
    28321 ctgtctatga tgcaggttct gaggccaggg agacgacaca gaagtatgca aatatgactg
    28381 taagggttcc ggaaaataga tttgaggata ttttcaacaa tattaaaggc atgggcaaac
    28441 tggtcagcga aaatataagc ggcagcgata taacagcgga atacagggat acgacagcaa
    28501 gggtggataa tctgaagatt caggaacaga gcctgcagca gcttatggca aaggctaaga
    28561 ctgtggatga gatactgaaa attgaatcgg aactgaacag ggtaaggact gacatcgata
    28621 ttaactcagg aaaccttaaa cgctgggata acctggttca gctgtccact atctatgtat
    28681 acatgagaga gcttaagccg gaagagctta aaagtgtgga tgtccccgga acgtgggaaa
    28741 aagcatatca ggggttcata aaagcggtca acaacgtggt ggaggggttt cagaagacac
    28801 tgatagtgct ggttgccgca ataccttatc tgtttgttgc cggagtgatt gccgccatag
    28861 ggtttttagc agcaaggaag attaaattaa agaagaagca ataaaataaa agtaaaagaa
    28921 aaagtgcatc gggagatgca ttttttttga tacgaaatat taatatagag gttttttggt
    28981 tcttatgtgg aaataatatg tattgtatgt gtatatgcaa ataactcacg gaggatttaa
    29041 ggaatggaaa aaggcaaatt cattatcatt gacggcaaca gcctgatgca cagagctttt
    29101 tatgcactgc ccaacctcac taattccaag ggctttcata caagtgtaat atatggtttt
    29161 gtaaatatga taaacaagat aaaagaagaa tacaagcctc aatatatagg cattgctttt
    29221 gacaccaagg caccaacttt caggcatcag gagtatgccg agtataaagc agggaggctt
    29281 aagatggctg aggaaatggc ggaacagatt cctgtgctga aagaggttct ggccgctatg
    29341 aatatcagac aggtggaaat agacggtttt gaagcggatg atataatcgg aacggttgca
    29401 gccagatgtg atggagaagg catacccacc tttatagtaa ccggagacaa ggatgcattc
    29461 cagcttataa gcgataatgt gcatacactg atgaccaaaa aaggaataag cgaaatagaa
    29521 gaatatgaca cagcaaagct gcatgagaaa tatggaattg ctccggctca gataactgat
    29581 cttaaagggc ttatgggaga tgcttcggat aatatccccg gagtgcccgg ggtaggggaa
    29641 aaaacagctc ttgagcttat acaccagttc ggaacggtag agaataccct tgagaatgca
    29701 tcggagatca agaagaacaa ggtaagggaa aatgtgtcgt ctaatatgga tcaggcaatc
    29761 ctttccaaga gactggctac catagtaaaa gatgttccca ttgattttga gcttggggac
    29821 tttgctttga aggaagcgga taaggagaag ctgtacagcc tttttgcgga actggagttc
    29881 aaggggttga ttgaaaagat gtgcggcagc aattcctgca ctgaagcagc agggactgtt
    29941 gaaattgctg taaaggaaat agacggtatt gatgagctga agaaggtatt tgaagaagta
    30001 tccaagtgcg gaaaatttgc attcaagata gatgatgaac tgggagaaat gctcagaacc
    30061 atatatatta atgcagatgg gaaacagtac cgttttaaca ttaatgacag taacatgccc
    30121 cttgttaagg agataatgga agattccggg gtattaaaga cgggccacga tataaaacag
    30181 gatatattgc tgcttagaag gtatggaata gatgtcggcc ctattggctt tgataccatg
    30241 atagccgcat acctgcttaa tccctcaaag ggctcctata ggataaaaga cctgagccag
    30301 gagtatctgg gctatggtgt ggacgggtat gatgctaagg gagcggagca ggttcaaaag
    30361 tacgcagcta ctgtaaaagc ggcggcggag ctgcagccgg tacttacagg aagacttgaa
    30421 gaatatggaa tgatagaatt gtataacagc gtcgaactgc ccctggtaaa ggtacttgcg
    30481 gatatggagc atgaaggctt cagtgtggat aaagccatgc tggaggtgct gtccggtgat
    30541 tttgcgaagc agatagatgc tcttacatca gaggtataca gactgtccgg agaagagttc
    30601 aatattaatt ccaccaggca gcttggaaca gtattgtttg ataaactggg gctgcctgcg
    30661 atgaagaaga ccaagaccgg ctattctaca gatgtagagg tattggagca gctttatgac
    30721 aagcatccga taattcagaa gctacttgag tacaggcagc ttgtaaagct taaatccact
    30781 tatgtagacg gcctcattgg cataataaat cccgaaacag gcaggataca taccaagctg
    30841 aaccaaacgg ttacagttac gggaaggctc agcagcacag agcccaattt gcagaacata
    30901 cccataagaa ccgaaaatgg aagacagata agaaaggttt ttgtaccgag gagccaggat
    30961 tatattctgg tagatgccga ctattcacag attgagctga gggtattggc gcacatatcc
    31021 ggagataagg gccttattga cacctttaaa cgaaatgaag atatacatac aaggactgct
    31081 tccgaggtat tcggaattga gattaaggat gtgacacccc ttatgagggg aagggcaaag
    31141 gcggtgaatt tcggtattgt atacggaatg agtgattatg gcctgtcaca ggatcttaag
    31201 atatcaagaa aagaagcaaa aaaatatata gataattact ttgccagata tcccggagta
    31261 aagcaatata tgactgatat tgttgaagct gcaaggacag acggatatgt aaagaccata
    31321 atgaacagaa ggagatatat tccggaaata acctccagga atgcggtgaa caggaacttt
    31381 ggagaaagaa tagccttgaa tactcctatt cagggcagcg cggctgatct gataaaaatg
    31441 gccatggtta aggtgtatga cgaattaaaa aagagaaacc taaagtcaag gctgatactg
    31501 caggtacatg acgagcttat tttagaaact cacaaagatg aactttcgga agtgaagaat
    31561 ctggtgaagg aatgcatgga gaacgctatc aaattaagtg tgcctgtaat tgctgatatc
    31621 agcagcggag atagttggta caatgctaaa taggaatggg tgggatgtgc tgtggttatt
    31681 ggtttgacgg gaggaatagc aagcggcaag agtactgttt cagccaagct taaagaattg
    31741 ggagcggctg ttattgatgc ggatttgctt gcgagggatg ttgtaagaaa aggggaaata
    31801 gcatataata aaatagttca gtgctttgga gcggatatat tgctgcccgg cggggatatc
    31861 gacaggaaga agctggggga tattgttttt tcggataaag aaaaactgga actgctgaat
    31921 agcataaccc atcctgagat aataaacaga atgaaggaaa gaatccaaga gcttaaggcg
    31981 gaaggagcca aggtaatagt agtggatgct gccatactga ttgagatggg attgcacaaa
    32041 tatgtggaca gtgtctgggt tttgtcggta gacagggaaa cccagctgaa aaggcttgca
    32101 gaacgggata aatatgatta cagagaagca gaaaacagga taaactcaca atttaccgac
    32161 gaagtaagaa aaaaatatgc ggatgtggta attgacaaca gcaaacccat tgaggaagtc
    32221 gaaaaaaggc tggaagaatt atggaataat attgctgggg gagaatagta tttggctaaa
    32281 gtcaggaaat taaagcgtat caggctatta gtggcagtat tgctcatata tgctgcagta
    32341 cttaacatac cgaatttact aaagctttta tatccaatag agcataaaga gataataata
    32401 aaatacggac aaatgcacaa tgtagatcct ttgctgcttg ccgcactgat aaagacagaa
    32461 agcaactttg aaccccgggc agaatcgaga aaaggggcaa aggggcttat gcagattaca
    32521 ccttcgacag gagagtggat agcaaaaacc ataggtgtaa atgattttaa cgaggatatg
    32581 ctttttgacc ctgagaccaa tattatgctt ggcagctggt atattgagca tcttacaaat
    32641 tactacaatg gcatcttcga attggttttt gccgcttata acgggggacg cggaaatgtg
    32701 gacaagtggc tgaaagacaa gaatttgagc agcgacggca ttacactgga taccattccg
    32761 ttttccgaga caaaaaatta tcttgaaaaa ctaagaaaga attataatat ttataagatt
    32821 atttattaac gggatgctgc cgatccgcaa cataaacggt ttgataaagg atggagaaca
    32881 agtaatgaag gataaaaggt ttttattgat ttttatattg ctaatgctgc tttcattggt
    32941 gtcctgcgaa gaatcagttg tgccgaagga aacggaaatt gccgtagaaa atgaggaacc
    33001 tgtggaaggc ggaacaataa atataagctc ggtggaaccg gggtcattga atccgctgct
    33061 caacaaaagc aagtcctata atgagatatc gaagttgatt tttcaaagcc tggttgaata
    33121 tgatgcaaat ttgaaaatta ttcctgttct tgcacagagc tgggatttta tggatggctc
    33181 caacaagttc gtgataaagc tgaaagaaaa tgtgttttgg agcgacggag aaagcttcac
    33241 tgcggatgat gtaaaatttt ctttggatac gattaaagca tctgaagatt ctatatataa
    33301 gggcaatttg gagcatattt attcttataa agtaaaagat gcctcaacaa tagaaataac
    33361 cttcgaccag ccctttgtaa ctgcgattga tatgctgagt tttccgataa taccccggca
    33421 tatttataaa agcaatccga atgctgttcc gatagggacg ggtatgttca aggtttcgga
    33481 gtacaacagg ctcaaatcca tggaactggt atacaatgac aagtggaatg gcgaagaaaa
    33541 gccatatata gagaaaatca gaataacttt cataaatgat gttgatgcgt tttccatagc
    33601 ttttcagtct aaggaactgg acctgttgaa cacaacctcc tatgattggg aaaagtacag
    33661 cgaaatgaaa gatatcaatg cctataaata tacaaccatg tattatgatt ttatcggact
    33721 gaattacaat aatccaatat ttcaggataa agcagtaaga aaggcaatga ttcaaggtat
    33781 taacagaaag tcaattgttg ataaatattt gcttgggaat gcagcagtta cagacgtgcc
    33841 gattaatcca acttcctggt tgaaggatgc agatgccgaa aaatacggat acagcagatc
    33901 tgaagctcaa aatcttttaa tggctgcagg ctttgcagat ttaaataatg acggcatatt
    33961 ggagagaaaa gttgcagaag cagtacagca gttgagcttc acgcttttga ccaatgacga
    34021 gaatgaattc aggagaaaag ccgctgagga aataaagaaa aacctggagg acatcggatt
    34081 tcgtgtcgaa ataaaatttg ccggatttga cgatgtaaaa gccgccatag aaaccggtga
    34141 atttgatgca gtacacacag gctacaacct gtcaaatacg caggacttat cctttgcatt
    34201 ccacagcaca caaatagaca gcggaaaaaa tttcatggcc tattcaaacc ccgaaattga
    34261 tgcgcttctc ctgcaagcat atactgcaat ggatgacaac aggcggaagg aaatatacaa
    34321 gaacctgcag aaaagcttca gggaagaggt tccgtgcata agcctttttt tcagggaagc
    34381 cgctttggtt gtgcgggaca aaatacaggg agaaataaaa cctgacacgg tgaatccata
    34441 tagaagcata cataaatggt tcgtgccgga aagtaagagg tagaaagaac cattacaatc
    34501 ctattgacac ataagccatt aggttatata ataatctatg tgacacgcgg tcgtggcgga
    34561 actggcagac gcgcacgttt gaggggcgtg tgggtgaccg tatgggttca agtcccatcg
    34621 accgcaccat aatgaatatt gactttaagg ctatgagctt tgaagttttt tattttttgc
    34681 actttaaaca gacaagccta tcaccactat aatatgcaga tttcttggca attcatatct
    34741 gatttcatat ttttatgcaa accttttaat atcacagggt actttaatat tcctgaaaac
    34801 taaagtcaaa acatcccagt ttttgagcat agggatggtt tggctattat ttttcatctt
    34861 tttctcagcc cggaattggt cggatctgca gatgattaaa catacctatt tatcggtatt
    34921 tgctttgtcc gcctgggtag gcacagctat gatcaggtca gccaaaaggt acggaaagct
    34981 tgctcttttt ctaggtttgt taaatatagc ctgggttgtc aatacggtta ttttttcata
    35041 tattttggag ataccccaga ttgcccccta tattgtttca caaataatct tacttttaaa
    35101 cgcaataggg cttattcagt tttttttaaa ggagcaaaga aatgagattg agcggcgatc
    35161 agatcagatt gcatacctta cattccatga cgagttaacc ggtttgcata atagggctta
    35221 ttttgaaaag aaaatacaag agctgtcaaa ggacaatgac tgcctgcccg tctctttgct
    35281 cattggagat atgaacggat tgaaattcat taatgatgtt tttggccatc agaagggaga
    35341 caaatggctt aaaaggatag ctgccattat tcagcaatcc tgccgtcaaa atgatattat
    35401 tgctaggtgg ggcggcgatg agtttgctgt tattatgcca aacacggaca gagaaaaggc
    35461 tctcgatatc gcacatgaaa taaatgaggc ctgtaaaaga aatcaggagg aggattcttt
    35521 tttcagtata tccctgggtg tggcaactaa aaatgacagc gggacagatt tgtcaaaggt
    35581 cttaactgag gctgaagaat taatgtacca aaacaaactt atagaaggta agaaaacaag
    35641 gttggctttc gttgaagtat taggcaatct gctgcataaa aaaggctatg aacccaaaga
    35701 gcatattgaa agactggcag ctttggctaa agaatttgcg gcagttctta acctttcaag
    35761 ggaaaaccgt aaatacctcg tgcagactgc aaatttgcat gacataggca aaatcggcat
    35821 acctgaagat atagttctga aagagtcccg gcttgatgaa tcagaatggc ctattatgaa
    35881 aaaacatgtt gaaatcggat accgattagc ccagatttcc ggagaatttg cccatttagc
    35941 cgatgtaatt ctatatcacc atgaatggtg gaacggccag ggctatcctc atggactgaa
    36001 aggggaagat attccccttt tgtcgcgaat catatccatt ctgcatgctt ttgatgttat
    36061 gacccatcag cagccttaca aacctgccgg gactatagac gaagctctgc atgaactttc
    36121 cctgaatgcc ggaacccagt ttgatcccgc tttagttcct gtttttattg atatgatatc
    36181 tgatatagct ttggataaag actgcaactg atttattcct gaggcttgga gatatttctt
    36241 tttttacgct gtttcacaca ttcggtcaat aaaaactcta tctgcccatt gatggagcgg
    36301 aagtcatcct cggcccattc cgccagttcc ttccaaagag atgatgataa tctcaggggg
    36361 atttgctttt tttctttttc tttgttatcc atatcatcat ccctccattc tatatattag
    36421 cataatgata tattagtata atgatccgct gttgacaatg ggttgagcat ctttgttacc
    36481 gcagaggacg accagcagat tgcttaccat tgctgctttg cgctcttcat caagctgaac
    36541 tatgtcattt tcgctcagct tcgcaagagc catttctacc attccaacag ctccctcaac
    36601 aatcatctgc cttgcgtcaa tgattgcgga agcctgctgc ctttgaagca ttgcagcggc
    36661 tatttccggt gcataagcca aatgtgtgat tcttgcttct ataatttcaa ggcccgcgat
    36721 ttccactttt ctttgaattt cttcccttat tttatccgct atttcctggc tgcttcctcg
    36781 aagagatttt tcattatctt cgcttgaagc atcataggga tacaatctta caatatttct
    36841 aagtgccgag tcacattgta tggaaagata ttcaatgtag ttatccacat tgaaaaccgc
    36901 ctttgcggta ttaactacct tccatatgac aacaattcca atgattatgg gatttccaag
    36961 ctggtcattg attttttgct tgtcgttact caaggtcatt gctttcaggg aaatgatctt
    37021 tttgtttctg aaaacggcat ctgctgcctt acccgcagtt ttaccggcaa gtggtgaaat
    37081 atcggcggat aaaacggctg tagcagctgc atgggtagca ttgttggcag ctgctgcaaa
    37141 aggattgaca aagaagaacc cggcaccctt gagagtaccg taatatttac cgaaaagcgt
    37201 cagaacaaga gcttcattag gtttaagaat ttttaaaccg atgaataata ggggtccagc
    37261 cacaaatgca taaataacac agactattat tagtatcact cctaaaataa tatttttccc
    37321 gctatcgatc aggtttacgg caaaaataaa caaagcaata gaagcaatat aaagtattat
    37381 gtttacaaca agcatcagca tgccgttaat ggttttaggc aatacctcct gataatgcaa
    37441 ttggttttca gcaacgtttg atttttcgga cattttaatt aacctcctca tataaatatg
    37501 atatcaatat gatatcaaat ttatattata ccaaggttac taaaatgtca agcaattttt
    37561 gcatcaagat gcgctaattt tatttgtttt tctccggttc gtaggcgtct tcttcataat
    37621 caaattcggt ttttttcccg gttgctttat ttgtaaagcc tctgtacggg ttattgctct
    37681 tgcttccatg cttcaggcgg tcataataat cctcttcgta atcaaaatct ttttttatcg
    37741 gtttctttct cctatccctc aagatactca tctccttgca ataaatatta tatcaaatat
    37801 tgaaatttag tgataaaata ttggtatatg ctatgcttaa tatgcggata gcgtaagact
    37861 gccggactgt acggttttat tagggggtta acatgaaaag ggcaattgga ataatgatta
    37921 tggtttttat atttgcagcg gtactgacac tttacgggct tgcagaagaa gtcccgtaca
    37981 agggagccag cccctgggct gtgccggagc ttgataaagc agccggatac gggctgatta
    38041 ctgacaggat caaggataat atgagtgtac cgattacaag ggaggaattt gcggaaattg
    38101 ctgtaagact gtatgaaata aataccggaa aggcagcaga atattccgat acggatgttt
    38161 tttccgatac tgacaatccg gagatattaa aggcatacag cttgaagatc gttaacggca
    38221 tagatctgca aaggaggctc tttttaccaa agacgttcat taaccgtgaa caggttgccg
    38281 caatgatgtt caggacaata caggcagctc ccctgaattt tgaattccct gcagggaagg
    38341 gtgggatatt tgtagatgaa aatgaagtat ccgattgggc tttagaatct gttaaattca
    38401 tgactgagaa cggttttttg aggggcgccg acggaagaat aaatcccaag ggtacctgta
    38461 caagggaaat ggctgtacta attgcagcca ggatatacga aacatacttg tctgccgctg
    38521 aagcgcctgc tgaagagcct gctgaagagc ctgctgaaga gcctgctgaa gatgagggta
    38581 cgacaggcag cgagcccggc accggatcct atgactggga tcagatagtc ataaacgata
    38641 cggtaatatt cagggataat tattatatca ggaacaagga tgggttctat tatattttta
    38701 taggggccga gaggttaaaa tatgccttta agctgcgcaa tgccggaagc tatacctatc
    38761 ctgaggttga agttatcgga ggcagcataa ctgccgaatg gagcagcgga gagaatgtcg
    38821 tattgaaggc ggaaatgcgg gaaggcagca ctgaagccat agtcggcggt aaaatagttg
    38881 atataggtat ggctccatac attatcgccg gaaagatgta tattccaatc aatctcttca
    38941 tatctgccct ggagatggat gtggaaacca gcagcaatga taatatactg ttcatacaat
    39001 acaaggatgc ctttcctgca gaaatacttt caggtatctg gtcagataca ggcattgacc
    39061 tgtttgcagc tgcaaaggat ttagaaagcg gttcagggaa ccttccgtct tatgcgacag
    39121 cttacaggtt caacagtgac ggtacctatg aactgataaa ggtcagtgcg gcaggtatca
    39181 acgacaccat aatcaaacag aggggcaaat ataaggttat ggggaataca atcatgtatt
    39241 atgatatatt tgagacgatt tataaagggt ctcccttcag cttgaaatat agggacgaac
    39301 cagtggatag gccccattat gagtttgttt acaattatga ttctaaggag gaaataatag
    39361 aaataggcgg attctggctt aacagggttc agcaatttcc gaggaaataa tgcgagattg
    39421 aagatgggtc attctattgt aatgctgccg gcattgtgat agaatcagaa ttaattgaaa
    39481 agagaaacag ttcaggtgca taactgcagg caatttctgc agaagcttaa aggggaaatg
    39541 ggtgaaagtc ccatgcggtc ccgccactgt gaaggtgagt atcctagaca tgccactgga
    39601 gtttctccgg gaaggcctgg gaatacgacg aacctgagcc aggagacctg cctgaatctg
    39661 aagcttgcct acgagagata gggaggtgct taatcgaaat ttttgttcct tctatatcag
    39721 tagaaggaac tttttactta acatgaatca tggactatat ttaatgggag gattgtcatt
    39781 tgaaaaagat tataatgctg ttaattgcat gtataacaat actgagcctt tttggctgcg
    39841 ttgatactca acagaaaaaa gagtttgagg atgcggggaa aattgaagtt acggatatta
    39901 aaggcaggag catagtgctg gataagattc cccagagaat agtctccctg tcaccctcca
    39961 gtacagaaat actttttgct cttggggcag gggacagggt tgtcggggta accaactact
    40021 gtgactatcc tgaggaagcc aggaaagccg aaaaaatagg ggattatgat gagccgaata
    40081 tggagcttat caggaaggta cagcctgatg ttgtgctggc tggtcatgta aaggaggaag
    40141 ttgcagaggc ccttgagaag atgggtattc ctgtaattat tactgaagcg gaagactttg
    40201 atacaatata tcacagcata aagcttgtag gccaggtaac cggaactact gtccaggctg
    40261 aagcaatcgt aaacgggatg aagagcaaaa tagaggaaat agaagcaaag tttaaagata
    40321 aggaaaaacc cacggtattt tatttggtgt ggaaggaccc tctttttaca gccggctcag
    40381 aaacctttat taatgatgtt ataaaagctg ccggagggat aaatgttgcc gaaaaagttg
    40441 aaggctgggc aaattacagt gcggaagaga tgataaagga taatccggat atgctgatag
    40501 ctgccctcca ctctacggat gaaggtatga ccagggagga tctgtcaggg gacaggttat
    40561 tcagccttct tgaatgtgta aagcagggga aggtatatat tatgcctgat gataacataa
    40621 tatccaggcc ggggccaaga atagtacagg caatagaaga tatgtctaag gttttgcatg
    40681 gagaatagac aacaggttac ggggtacgag gtacggggtg cgggggtacg aggtacgaga
    40741 tgcggggctg cgggcttttt ggaggtgagt ttaactggat tatcagataa gtgttgaaaa
    40801 actgagcttc agctatgatc ttaaaaaggt tcttaatcag gtggaaatga atatcggtaa
    40861 agggagtttc ttttgcatag tggggcctaa cggatcgggc aaaagtaccc ttctcagagt
    40921 catttcagcc gctttaaagc ctcaggaggg tgctgtgtat cttgacggca gaagcatcaa
    40981 tggtattaag catcgttcca ttgccggaat gctttccttt gtgcctcaga atacttctct
    41041 tgaatttgat ttcaaggttt ctgacgttgt gcttatgggc aggtatccgt atattaacaa
    41101 actcaaggga gagaccggaa aagattttga gattgcagaa aaagctatgg aatatacaaa
    41161 tacaacacac ctgaaggaca ggagctttat ggagctttcc gggggagagc ggcaaagggt
    41221 gattcttgca caagcatttg cgcaggatac ggatatactt atacttgacg aaccggtatc
    41281 ccatctggat cttcagcacc aggtggaaat actgaatctt ataaaaaaga tgtgtgttga
    41341 caggaagctt acggcaatta ccgtactgca tgatcttaat ctggcttccg catacggaga
    41401 ctatattgta atgatgaagg caggtgcaat aatgaagcag gggaccccct tcgagacact
    41461 gactgctgac ggcatcaaag aaatatttga tactgatgta tatgtatcgg tcagccctgt
    41521 aggaaacaag ccttacatat atgccctgac caaacccaat atagagaaaa agggaatacg
    41581 ggttcatgtc atatgcggag ggggcagcgg cagcgatata ataagcaggc tgtgctccga
    41641 gggttttgac ctcagcagcg gagtgctggc tatcggcgac cttgactgga agatttcaaa
    41701 ggaaaatgat atgcaggttg ctgaagaggt accttttgta ggaatatccg gggaagccta
    41761 tgccaaaaat aaggagcttg ccatggctgc cgatgccata gtgctgaccg gattgtatct
    41821 gggcaaatca aaccttaaga atcttgaact cctgcttgaa aaggacctgg aaggcaaacc
    41881 tctgtttata ctggaggatg agagctttgc agccagggat tatacggggg gtgccgcatg
    41941 caggctgtat gaaatgataa agttccggaa caattcaata cttacggaca gcaaattatt
    42001 gaaggaagaa attataaagg cggtagaaac tcatggggaa agtaaagaat agaaactggc
    42061 aggtgtttgt atttctgatt gcctctctta tattaacgct gatttttgca acatcaacag
    42121 gagctgtaaa aatcagtaca gcggaaataa ttgaaatact ggcaaaaaag gattctgata
    42181 tagtcaacgc ctctattctt atggatataa ggcttccgag ggtactgctt gcagttgtct
    42241 tgggagccgg gcttgccgca gtgggaggag tgatgcaggc gatattcaag aaccccctgg
    42301 tggattctta tactctgggg atgtcttcgg gcgccgccct gggtgcggtg atttcaatta
    42361 tcacaggcct gaatgcaagg attttgggga tggagacaac aggagtattt gcatttattg
    42421 gtgctgttct cacgctgttt ttcgtataca gccttgcata tacaagaaac aggatgtcca
    42481 tcaattcctt tctgctggcc ggtgttgcag tgagctattt tcttgcatcg gtgatatctt
    42541 tccttatgat gctgaatcac gataaaattg agcatatagt cttctggaca atgggaagct
    42601 tatcaatggc aacctggaat gagtttttca tatctgcagc acttataata ccctctttgg
    42661 cagtgctgat gtattatacc agggagctta acatactcac gatgggagaa gatgctgccc
    42721 atcatttggg cataaatacc tttgcattga aaaacgtact tctcataacc tgtgcacttg
    42781 ttgtcggcag tgtggtttcc accggaggga cgatagcatt cctgggactg gttgcccccc
    42841 atattatcag gctgattata ggctcagaca ataaaagggt aataccttac tcggcacttc
    42901 tgggatcaat actcctggtg ctggcggata ctgtcggcag ggtgctgatt cagccggcgg
    42961 agattccggt gggagtgatg acttcgataa tgggcggccc ctttttcata ttccttttaa
    43021 gaaagtcgaa aaactgaaca atatcagaaa gatgtgacac aatataagac aataagtatg
    43081 gatttcagat caagcggaat ggtgacggtt caatagatat tgtcttcaag aatgtgaaga
    43141 gttatattaa agataaatga actcgtccag ccgcggctga acgagttcat atttttgcta
    43201 ttttattatt tttttcatgc actgaatggt atagtcgata tcttccctgg taatatcgtt
    43261 gttggtaacg aatctcataa gtccgccttc agcgccgttt atctttattc cttttttgaa
    43321 taattgatct tcaagcttgt ctgaacttat tcctgtctct tccatgtcaa agaaaaccat
    43381 gtcaatatga acatcatcat aattaagctt tattcccggt atcgacataa ggcgttctgc
    43441 caagtatttt gcattttcat gatcaatgtg aatgcgtttt gacattttct ccaaggcaat
    43501 gagacccgca gcggcaagta ttcctgcctg gcgcatcccg cctcccagta ttttccggtt
    43561 ctttctcgcc ttttctatga attctttgct gcctgcaagc atcgacccta cgggagcgca
    43621 caagcccttg gaaaggcaga acatcacaga gtccgtatat tgagtaatct gtgccgcatt
    43681 cacttccagg tatgctgcgg cattgaaaag ccttgcaccg tcaagatgaa cagggatatt
    43741 gtggtctttt gcaatagtat atatttcttt cattacctca aggggtacaa cagttccgtt
    43801 tgacaatgcg ttttccatgc agataagtcc ggtttcaggg tagtggatgt cattgccgcg
    43861 aattgctttt aaaacatcct ggggattcat gacacccttt tcccctctga ttgtcctcac
    43921 cattgcaccg gacaatatcg ctacagctcc aacctcgtgc tgtattatat ggcagttttc
    43981 ttccgcaata atctcatttc ccctttttgt gtgggtcata actgccagct gattccccat
    44041 ggtgccgctg ggtacaaaaa gagcagcttc cttgccggca tattctgcgg ctttttcttc
    44101 caaaaggttt acagtaggat catcaccgta tacatcatca ccaactattg cttttgccat
    44161 tgcctcccgc atttcattcg taggctgggt aaccgtatca cttctaaaat ctatgtatct
    44221 caatgctaaa acccccatat attttatata aattattata cagcatttgt attggctgta
    44281 caacataaga agaatgcgac gcgaggacac gggacacggg gacgtttccc ctgtcccact
    44341 tccagtggga caggggaaac gtccccgtgt cccgcgtccc catgtccccg tccccgtgtc
    44401 cccgcgtcgc atcattcttt tgttctttat caacatatag cttaatacaa atatagtaat
    44461 ctaagaaatc agttttatag gagggggtca acaatgtcaa aaaacaaatt aaagcttata
    44521 tgcgtttttt ctttagtttt aataatagcc ttatattctg tactttttta tgccggaagg
    44581 cttccggcgg catataccgc atttacggaa aacatcggaa tgtcaaaggt ctcgaggcag
    44641 cttttcgagg cttacaatgt gatggataat acatttcatt ttcaattgcc cgattcatgg
    44701 tccgcatacg aagcatcctt tgcaggggag gaaatactat atcatctgaa tttcatttcc
    44761 aaggataaga agatacatgg ttttgtccag gtgtggaagc tctccgagcc gttaaagcag
    44821 tttattgaaa agtcgaagga atccgctgta ggtgtaatcg atttcaaaaa tttcgatatc
    44881 aaggaaataa cggttgataa caaaaacggc tatcttatgg attacagcag ggcaaatcag
    44941 aaaggggagt atataagggc ttatgaggca tttattaagg ggttttcaaa caaggtgtac
    45001 agattaagct tctttgtgcc ggaaaaagag tggaaagaat attacagaat cctttttgac
    45061 aggataatac atttggtaaa gattagcaaa taggcaatat ttaacaggcg caaaggtcag
    45121 aacgatatat ggaatcgatg ccgcagtatt ttgtttatgc taaaagggaa aaaatgtttt
    45181 atgtcgaata atcatataat tatgattatt ttttgtaatg gttaattctg agcatgttag
    45241 gaggtagtca gatgccaatt aacttagtgc tgtgtgagga caagcatatc atattgaata
    45301 gtatcaaggc aagcctggag aaggctgcca aacaaaacgg aatagatgcg aatatttgcc
    45361 tcatgactga caatcccaaa aggataatag aatattccag acaatactca caaggtgtta
    45421 acgcatattt tcttgatatc aacctggaaa cagatatgaa tggactggag ttggccaagc
    45481 aaataaggag ccatgacccg cacagctaca taacctttat tacagggcat ctggaattgt
    45541 gcatgacggt gtttaaatac catatcgaag catttgaata tcttgttaaa ccggtatcat
    45601 accaggccat agaagaatgt ctgatttcaa tagacaagca atatagccaa tacttgagca
    45661 atcaaaaata caacaaagat gcaatgatca taatcaggtc ggggatctgc gattatagag
    45721 tggaactcta tgacatcata tacgttgagt cgataaatca aaagcttgta gtgcatacca
    45781 gaagccgcaa cattgaattc ttcggatatc tgaaggatat cataaaagat ttgaacaaga
    45841 acggagaaag cttttaccga tgccatcggt cgtacataat caacattaac catgtaaaag
    45901 aggtaaacta cagggattca agcattatta tgtccaatgg tgaaaagtgc tatatgtcca
    45961 ggcagcaaaa aagcgaaatg cggaatatat tgggcaggat gggtcaggat tatgatgata
    46021 ttatataaaa tatttataaa tacaattggg atggtactct ttgcatattt ggcgcaggct
    46081 ttatacaact ttcaaaggga aaagaaagta tttattaaat ttattattta tttagctatt
    46141 tgtacaaata ttttatttta tcttgacaaa tacattggag caggagtccg tgccatagct
    46201 gttttgctta tgatgatgtt aatgtcttat tttgttttga aacttaacgt gctccaatca
    46261 atagttgtaa atataaccaa tggaattgcc cttgtaattg gagatatatc tgctgtattc
    46321 attatggtaa aaatatatgg atatacccac gaaatgataa agtcaaatat aatgctatcg
    46381 ttgatagctg acttaattat atttggtatg tacataataa taattctttt aattaaattt
    46441 tttaagcaga ctcaggaaat ggcagacaaa tataagcgta aaaatagtat aagaacgtcc
    46501 ctttatatgc ttgtgacatt tataataatc gtggttaatt atacgacata tataaacttc
    46561 attggagtgg caagtcaaaa cattattctt ataaatgttg caatcatgtg gctttatctt
    46621 atactgagtc tctacattaa ctttacaaac agcgctctgg cactgaagga gcagcagtat
    46681 gaccagcagc aggactatat aaggaccata gacaacctta ttaatgattt taggagatta
    46741 aagcacagct atgccaatac catatacggt tttttcggat acatccagga ggatgacctg
    46801 gaaggcctga aggcctatta cacagaagtg gcggatgagg caaaaaggat ggacagcaat
    46861 ctgcttctgg cactgcagcg gataaaggtg tatgcggtgt tcgggctgct gtggaataag
    46921 ataaatgaag ccgagggcag gggaatagaa gtgggtatcc gggtaaccaa tgaaatccgt
    46981 gaagtaggta tgaagcttac agagctctgc gaggtgctgg gaaactacct tgacaatgcc
    47041 attgatgcgg cagcggcttc aatggataaa aaaatgaata tcactctgac cgacggtggg
    47101 gggtatctga caataagtat tgagaataca tacgagggag atgtcgatgt aaaggagata
    47161 caaaagaagg ggtattccac aaaaggaaat gaacggggct ttggattgtc aattaccaat
    47221 catatattat ccggatatac aaatatactt cataatacct ttgcggaaga tgggatattc
    47281 aagcaggaac tggtgataaa aaaatgagag cgcatgaagc gctctcattc tttaactttt
    47341 agtctctctt gataagtgat tccggcatct taggctctct tagcgaccac atataacatg
    47401 cagttgttga ggtgcttgct ataagcaaag ccaaggtact gataagaatc attagcggtt
    47461 taaggaatct tttcattttc atacctcccc attattactt attttcatac ctgcaccata
    47521 catgctgccg gccagcctgt acgtaacggg tagtattaag actgattcaa acaataccgc
    47581 aaaggttatg attgaagaag cttttgttcc taagctgtac atagcgattg cataaagaac
    47641 caggatggta ataacagaca atattttcag cttcttcctc agttttctgc tgacaatagg
    47701 ccgctcctcg gtatctgccg gtgcatatct cagcattgct ccaaagcata ggataaagat
    47761 aaaggttata atatatatat tcaactttga aatcattcca aggtaagtaa ttccgaaaaa
    47821 gacaagtgcg cttgtgggaa ggcatgtcca ctcacgcttt gcatgaactc cggaggcaaa
    47881 ggtgcgcagt gcgccgaaac tgacgaaggc tattagaaaa tattgaaata tgtttaacag
    47941 catggctaat atgaatataa agaccatttt gtatgagttt gcgataaatg cggcaatgcc
    48001 atagtctatt tcggcaagtt tttcctccga caaatccttc agctctgctg caatagcttt
    48061 tgtaattttc ccgcttatcc tttccggaaa ctccataata tcaaccttct ttttcttgcc
    48121 tgacttagat actaatttaa taataagcac acatatgttg ggattcaata gttttataca
    48181 aaatgcccac attatgatat aaaatgtaga aaaatgccga attgccaaga atattgacac
    48241 ttcatcaata attaataatg cagatgggaa agttaattcc aatgcatttt ttttactttt
    48301 attggtatat taaatatgtt attaaaaatt atgaaagcag ggggatacgg cattgcctga
    48361 atctgaaagg ggaaaccttg taataattgg aggagcggag gacaagaggg ccgataaggc
    48421 aatattgaaa gaagtcatca gactgtccgg agggaaagat tcacgggttg ccttgattac
    48481 tacggcaact aacagccctg agaagtcggg gcaggaatat aaaaagctgt ttggggaatt
    48541 gggagccgga agtgtggtgt caataaatat aacagacagg gagactgcaa ataatcttga
    48601 gggtttgaaa gtactggagg atgcaacctg catatttttt gtaggaggag accagcttag
    48661 gatttccagc atattgggcg gtactgaagt gcatagatta ctgaaacaag catataaaaa
    48721 cggcggagta attgcgggaa ccagtgcggg tgcttccatg atgagcgaaa taatggttgt
    48781 ggaaggaaag gatgacgagg ctccgagaaa atgtacgctt aaaatggctc ccggtatggg
    48841 ccttttggaa ggagttataa tagaccagca ttttaatcag aggggaagga ttggaaggct
    48901 gctggcagct gtggcgcaaa acccgaatgt tttgggaatc ggaatagatg aggatacagc
    48961 catgatagta aacaagaagc tggaattttc ggtggaaggc tccggggtag tcacaattgt
    49021 agacgggaga ggaatctcat ttacaaatat atcggagcag tatcaggatg atcctcttgc
    49081 cataaccaac gtaaaaatac acattttgcc aagaggatac gggtttaatt taataaaaaa
    49141 acaggaaata atatttagga gatgaaagaa aatcattttt ctcaaaaaca caaggaggaa
    49201 gtattgtgat tattgtaagc agtcatgctt atcaaggtaa aaacatttat agttataaac
    49261 ctgtggtgaa gctggtagta gacctgggta aatacgcaga cgctccgacc aaggatattg
    49321 agggttttaa tgatgcaatt caggagcttc tgccgggttt gaaaaagcac tgttgctgca
    49381 aggggcatga gggaggcttt attgagaggc ttgaggaagg aacatatttt gcacatgtaa
    49441 tggaacatat tgcacttgaa ctgcaagcca tgctgggata tgacataaga ttcggcaaga
    49501 caagggagac cggcaaaaaa ggtgtttaca atgttatata tggatatgaa aacgagtatg
    49561 cgggcttgga ggcaggcagg cttgcatttg aaatcataga ggaaatattg gaaggggagc
    49621 tgccggatgt aaatacaagg ctggaagaaa tcaaaaaggt ttgtattgcg acagattttg
    49681 gggtcagtac tgctgcaatc gtaaaggagg cacagcaaaa ggatatacct gtgatacgct
    49741 tgggaagcgg gagtattctg cagctgggat acggcaggaa tcaaaaaagg atagaggcaa
    49801 cactgactga gaattcaagc tgcgtatcgg tggatattgc ctgcaacaag gaacttacca
    49861 agagcatact gaatgaatat gggatacctg ttccggaagg gaaggtattt aagaatgagc
    49921 agggtgcttt ggaatactgt gaaaaaatcg gctatcccgt agtagtgaag cctaattacg
    49981 gaagccatgg aaaaggtgta tccataaatc ttaagaatcc tcaggaagta ttagaagcat
    50041 ataaaatagc aagggaatat gaagatacag ttctggtgga aaaatatata aaaggcaatt
    50101 attatagggt tctggttgtg ggaaataagg tggtggctgc ctcacacagg atttccgcca
    50161 atgtaacggg agacggagca agcaccctga gagaactgat caaaagggaa aacagcaatc
    50221 ccatgcgcgg agaaggccat gaaaagcctt tgacgaagat aaacatagat aaagtagtgg
    50281 aacagcacct gaaaaaaagg aacatgactc ttgagtacgt tcctgatgat ggagaaatag
    50341 tgtatctacg cgaaaatgac aatttgagca cagggggagt agcaattgat gttactgatg
    50401 aggtacatga gcaaaacaaa aaactgatag cattggctgc cggtatagtc ggcctggatg
    50461 tggcgggagt tgatatcagc acggttgata tttccaagcc tctgactgaa acaggaggag
    50521 caataattga aataaatgcc gcaccgggta tcaggatgca ccattatcca tataagggca
    50581 cccccaggaa cgtggcaaaa gcaatagtgg atatgctgtt tccggaaggg agcaagagca
    50641 ggattccaat agtatcagtg acagggacaa acggaaaaac tacgacttca agaatgatag
    50701 ctcatataat aaagcggatg ggacatactg taggaatgac taccacaagc ggtatctata
    50761 taaatgatga gctgataaaa tatggggata acacaggacc cataagtgca agaacagttc
    50821 tgatggacag aagggtagaa tatgctgttc tggagactgc caggggagga atagtgaaca
    50881 ggggactggg ctacgatatt gctgacgtcg gtatagtgac caatatcact gaagaccatt
    50941 tggggattga tgatattaat acccttgagg atttggccta tgtgaaatct ttggtagcag
    51001 aagcagttaa acagaacggc tatacggttt taaacgccga tgatcaatac tgccttgaca
    51061 tgagggaaag gattatatca aatgttattc tgttttcctt gtcaacagat aatgaaacag
    51121 taaagaacca tgtgaaaaac ggcggaattg ccgtatacag caacggcagc agcatatttg
    51181 taaataaagg aggggtggag ctgccgttta ttgaagtaaa agctattcct gctgccatga
    51241 atggaatact gaagcacaac ataagcaaca gcatggctgc aatagccgga gcaatagggt
    51301 tggatatccc cattgaaagt ataatagcag gactttcaac ctttagatgc gacagcaccg
    51361 gtaatcccgg aagattcaat attcatgata tcaacggaat aaaggtgata ttggactatg
    51421 ggcacaatat tgacggttat agggtggtta ttgattccct taagcaaatg aaatccggct
    51481 gtcttattgg aataatcgga gtgcccgggg acaggacgga ctcaagcaca atagccatag
    51541 gcaatatttg cggcaacagt tttgacagaa tctatataaa ggaggacaga gacaagagag
    51601 gacgcgaacc gggggagatc gcggcactgc ttgaaaaagg ctgcaggatg ggaaacatta
    51661 agccatcgga aatctttatt gagcttgccg aggaaaaagc gctgaaaaag gctttcctgg
    51721 atgcggtgcc gggagatatt attattgtat tctttgaaga ataccaattg ctgatggatg
    51781 ttattgaaag aataaaactg gatctgttga aaatccggca gattatagca tgagagaaca
    51841 cggaggcgag gacacgggga cgtttcccct gtctcactga aagcgagaca cgcgaaacgt
    51901 ccccgtgtcc tcctcatgtc ctccatgaat atatattact agtatttttt ttttaatatg
    51961 atactttttt actatagaat agcatgagct ggtagtatat aatataataa aatatcaaat
    52021 aatttgacag catttaccga catttgcagt attattatat gtcatagggg tggattgaat
    52081 ggatatacac agtggtgata ccttggtttt cgctgttgac aacatgacta agagatggag
    52141 gatatcaaag gtagacaaca atgttgtgaa gatctttgag gaagacggat cgtacagaca
    52201 gatgccctat acatatctga tagaaatgca tgagagagga tatttaaagg ttgaaacccc
    52261 cgccatggta tggtaataaa gcagttatag cctattgttc ccaaacaata ggtttttttg
    52321 ttttttatgt cgaaaaatgt agatttttat ataatcatat aaagtgcgta ttgatgatat
    52381 agtttaaaag acgtatagta ttatttgaat atactttttg tgtgtgagag ggcctatgaa
    52441 agataataaa gtcgaaacag gctcatattt gataataata gccagcctgg tgttcatttt
    52501 gatttgcata ctattcgata agccgctgta ttttgggttt gccggggcag ttgcttttac
    52561 tgtgtcggta ctcattaaga aggggtatga tatcagaact ttgaacagct ttatgctaaa
    52621 cggtataaaa ggttgttcga ggctgatggt aattattctg cttacaggta ttgcagtgtc
    52681 catatggctg gcctcaggag tcgttcctac cttaatatac tacggattcg aatatataaa
    52741 gcatgctaat tacttacttg tatgttttgc agcagcagcg gtggtagcag tggttacagg
    52801 cacttctatc ggcaccataa gcactatcgg gatagcattg ttaggtatag ggaaaggatt
    52861 tgaaatacct gcgcatatag tattgggggc tgtagtatcc ggtgcatttt ttgcggacag
    52921 gacttcacct atatctggga ttgtgaatat tacattaaag actgtaaata taaaataccg
    52981 ggattatatt atcagcattt tgaaaacctt aatgcctcct gcggttatta ctgttgcagt
    53041 gtattatttt ttaggaaggg gatatatatc agtagtcaat cctgaaagga tagctgaatt
    53101 ccagcatagt atcggcagtt cttttgtcgt cagccctttt atacttttgc ttccgctggc
    53161 tgttataatt ctgtccctat tcggtattaa tattatattc aatatgagca taggaatttt
    53221 cgggggcgct gttttaagca aatttttgca gaatagtact gtatctgaag ttattcaata
    53281 tattatttcc ggttatcatc caaggacagg tattggggaa cttaatgaaa ttctaagggg
    53341 tggggggata ttgtcaatgg ttgaattgct tttgattctg tcaggggcag ttgcacttaa
    53401 cagccttctt gaaggtgcaa atatgatttc tcccataatc caaggagcag tgaaaaatgt
    53461 taagagcaaa gtatcaatga tatgcaggac agcagcttta agcagtctga ttacaataat
    53521 tacttgtgac caggtcctcg gagtcataat tctgggaaag attttgcaga acaaatttga
    53581 aacctttgga atcagcaaga taaagttagc caggaccatt gcggattcgg gaactgccat
    53641 cgcaccgctt atcccgtgga atgtgaacgc aataataata ctggccatta caggtatacc
    53701 aacaatgcaa tatgcacctt atgcgctgat gtgttacctg ctgcctgtca ttacaatatt
    53761 attctcattt tttaaagaga aaccatgatt taggtcagtt gttgatgaag cagcagcata
    53821 atagtatttg ataccagata atatgcatat tagtactata cgctaaagct tttaattgca
    53881 tatatcgata acttatgcta aaaatagaaa tagaatacta tatacgtcat gaataagact
    53941 tgtcacaaca taggaatcaa tgcaaatgtt cagtgcgtaa catatagatt ggaggatatg
    54001 caaatgagtg tcaaaatagt tgcagacagc agctgtgacc tgaatgaaga tttgaaaaag
    54061 gagcttggcg ttacgctggt acctcttact cttgaagtag gcagcaatgt ctttagggat
    54121 gatgaaaacc tggatgttaa atccatgcta aaggaaatgg cagcatgtcc gactcccccc
    54181 agaaccgcat gtccttcacc cagtgatttt attaaagcat atgagggcga ggatagcgtt
    54241 ttcgttgtta cattaagctc caaccttagc ggaacctatg aaagcgcaat gcttgcgaag
    54301 aagatgtttt tggaaaaggc aggggataaa tttattcatg tttttgactc cttcagcgct
    54361 tcaatagcag aaacacttat aagtatcaag atttttgaac tggcaaagaa aaaatgtgat
    54421 gaccttcaaa tagtagaaaa agtcaatgaa tacatcgaga gcatgaagac tttatttctt
    54481 cttgaatccc tggacaacct cataaaaaac ggaaggatga gcagggtaaa aggcggaatt
    54541 gcaacgcttc tgaacatcaa gcctgttatg ggggcaactg acgatgggca tataaagctg
    54601 gtagagagtg tcaggggtta cagaagggca tttatgcgtt ttgttgatat tataggggag
    54661 cagggagaga agctggagga taaggttatt ggaatagctc actgcaactg ccttgaaaga
    54721 gcggaggagt ttaagaaaga gcttctgaaa aaatataagt tcaaggatat aattatagta
    54781 gaggctgcag gccttaccag cgtgtatgca aaccagggag gcataatagt tgcattttag
    54841 cgggtataaa aggtacatta atgcagcttg atgtgtggca gtcaaaaata ggactatagt
    54901 gcgatagtcc tattttttat ttctgcctta agaaaatgga ataattttcg aaattatttg
    54961 taatgagggg tgataagttg aaaaggataa tatcagcaat tttaatagtc atgattttat
    55021 cgggatgttc ccaaaaacct gctgaacctg tggagacagc tgggcctgcc gtacagcctg
    55081 cagcagagcc gatagttgaa ccggaagttg agccggaaaa aactgaaacg gagcagaagg
    55141 ctgaagaaga aagcaatctg tttgatgcaa gaaaagcaaa ggaaggtgat atggtagccg
    55201 gtctgaaaat cattaaggtt caagtggatt ctcccgaaat ggaggattat gatgcatatg
    55261 tagattttga aggggaagtg acggtaagcg gcacatttaa gcataatctg aacgacgaat
    55321 tcttggatca tgaaataagc ttcaaggtcg atgaggaatc cgaggccttg ctcccaaagc
    55381 ttgcccatga ccagaggtat gtgtggttca tgttcatgaa ccacgatgaa gcggagaagg
    55441 cattaggagc tgcaggtacg gaaggaaagg caactgtaac aataaggaat tacagcatta
    55501 attatgctca tacagagata tggaatacgg ctgatttggt gaaagcagag atttcttccg
    55561 aataatctga gtaattcgag aatatttaaa aaaacccttg acgagaatat gaaaatgata
    55621 taaactaata aaaatagttt atacgactta taaatactgt gatcgggacg agtagaccat
    55681 gttcggctaa cagagacgga gatccgccga ctgaaagatt tccttggcaa gaatggttga
    55741 aaaccacccg ggagtagcat tctgaaatac cagtaagaaa tgacgccggc tgcgttaaag
    55801 ctttgagtgg gtttatacca atatgggtgg aaccgcggga gtcaggctct cgtccctttc
    55861 agggacggga gctttttgca tacccggcac ccctaaacaa aattacatat atgtatataa
    55921 cgatgatcgg gacgagtaaa ccatgttcgg ctaacagaga cggagatccg ccggctgaaa
    55981 gatttccttg gcaagaatgg ttgaaaacca cccgggagta gcattctgaa ataccagtaa
    56041 gaaatgacgc cggctgcgtt aaagctttaa gtgggtttat accaatatgg gtggaaccgc
    56101 gggagttagc tctcgtccct ttcagggacg ggagcttttt gtattttcaa atatcgtaga
    56161 ggagcaggtg atagatatgg tagaagtaag agttccggct acaagcgcaa atatgggccc
    56221 agggtttgat tgccttggag ttgctttgaa tctgtataac cgttttcaaa ttgaagaaag
    56281 cggaaacgga ttattaattg acggatgtga tgaaacttac aggaacgaaa aaaaccttgt
    56341 gtatgtttca atgcagaaat gctttgagaa gataggctgc aataatagat acaagggatt
    56401 gagaatcaac ttcgaaagtg acatacccat atcaagagga ttgggaagca gtgctgcatg
    56461 catactgggg ggagtgctgg cagccaatga aataggcaga ggaaacctga acaagaatga
    56521 gatattggag atcgcttcag agatagaagg gcatccggac aatgttgccc cggcactgtt
    56581 cggagggatg acagcatctg taaaagacgg aaacagggtt tattttgaaa aaattaaact
    56641 tcctccgggg ctgaaattct gtgcgataat tcctgatttt aagctgtcta caaaggaagc
    56701 aagagcagtc cttccggaca gtattccata caaggacggc gtttacaatg tgggaagagt
    56761 atccctgctt atagcagccc tttcaaacgg aaactttgag atgttgaagc tggcgtgcaa
    56821 ggataggctt catgagatat acaggggcag gcttatcggt aattacaacg aaattgttga
    56881 agaatgcaac aggctgaaca gtctttgtgt attcctcagc ggagcaggcc ccaccatcat
    56941 ggctgtttta aaagaggaag agacggattt ttgcagacaa atggaagaat atttatcaaa
    57001 gcttgaatgc aaatggataa taaaagaact gaagtccgat caaaatgggg cttgtgttgt
    57061 aaacatttaa ttaggaggat tattatgaga atcatagttc aaaaatttgg aggcacctcg
    57121 gttgcatcag gagaaagaag aaatttggtg gcagataagg taatatgtgc gatagagcaa
    57181 ggatacagcc ccgttgtggt agtatcagca atgggaagga aaggggatcc ctatgccacc
    57241 gatagcctct tatcactgat aaatgataag gattgcagtg ttgataaacg ggaggccgat
    57301 ctgctgatgt cctgcggcga aatcatttct gcagttgtta tggcagaggc ttttgctgaa
    57361 agaggatata aggcgagagt cctgacaggg ggaaatgcag gaatcataac agacgacaat
    57421 tttggcaatg ccaatgttgt aaatgttgaa gccgaaaagg tcttaaaggt gcttgagcag
    57481 gggttgatac ctattgtaac aggattccag ggaatgacgg aggagggaga cttcactacc
    57541 cttggaagag gggggagtga tgtgacggga gccctgctgg ggaaagcgtt aaatgcggaa
    57601 tatgtggaaa tctataccga cgtagacggt attatgactg ctgatccaaa tatcgtgccc
    57661 aatgcaaaga taatagacag gatagactat agagaggttt ttgagttggc gtatcaagga
    57721 gcgaaggtta tccatccccg tgctgtggaa tatgctttgg aagataatat acctttgatt
    57781 gtaaaaaata ctttctccga tacacccggt actgtaatta ccaatttggt ggaatacgaa
    57841 gaaaacctga ttacgggagt cacaagcatg acaaacaagg ccatggttat tgtaaatccg
    57901 agtgaagtat ttgaaggcgg ggctgaggct attttcaaac tgctggagag gaaaaaaata
    57961 agcattgatg ttataagtga atcccagtat cagaaggttt ttatcgtgga cagcgatgca
    58021 gcatccgagg ttgaggatat cttgaaacag ctgtgtgcag agtataggat cataaataat
    58081 tgcagcaaga tttttcttat agggaataaa gcaagcggca ttcaggaggt aatgtcaaag
    58141 ctggtaaaaa cattggaggt cagcaatgta aacgtattgc agacagcaac ttcgcataag
    58201 gacatttgct gcctgattgg agaggcagac aataaaaaag ccttgaacga gctgcatagg
    58261 gcatttttct gtgccggctg ataatataca gtatagttga aaggaatgaa gcaatgatgg
    58321 gaaaggttag aatagcatta ttagggcttg gaactgtagg ccaaggagta tggagcatat
    58381 taaacaccaa taaggatgat attttgagga aatgcggctg tgaaatagag gttgcaaaaa
    58441 tacttgtgag agatcctgaa aaacccagag gggtcaaagt gccggaaggg ataatcactg
    58501 ccgatgttaa cgatatttta aatgacagca gcataaaaat cgtgattgag cttatgggcg
    58561 gaactgagcc ggcaaaagat tacatgctta gggcaatgaa gtgcaaaaag catgtagtaa
    58621 ctgcaaacaa gctggtattg gcaacgcagg gagaggagct cttcaagact gcagagcagg
    58681 agagagtgct cttctattat gaagcaagtg tgggaggcgg tatcccgata atcagagaaa
    58741 tcaatgagag tctgactgcc aatagaattg acaagcttgt aggtataatt aacggaacga
    58801 ccaactatat tctgactaaa atgacccggg agggcatgga tttcaacgaa gccttgaggg
    58861 aagcgcagga gaaaggatat gcagaagcag atccctcttc tgatgtagac gggtatgatg
    58921 cggcttataa gcttgctata atgtcctcat tgtcctttgg tacgaagatt gatttctctt
    58981 ccatatacag ggaaggtata agcaatataa aagccgtcga tattgattac gcaaacaggt
    59041 tcggctatgt tataaagcta ctggcaatag gtaaggaagc tgacggaagg cttgagctga
    59101 gagtgcatcc tgcaatggtt ccaaaggacc atccccttgc aaatgtaaat gactccttta
    59161 atgctatttt cataaaggga aatgcggtgg gagatctgat gctgtacgga agaggggcag
    59221 gagaccttcc tacgggcagt gcggtagtgg gggatattat ttcaatcctg aggagcaata
    59281 ttgcgttatc tgcgctatac tccttcaaga acggcagggc tcaaaaagaa gtcaaaagcg
    59341 catacgagaa tgtatccgaa tactatatca gacttaatgt aaaggatcag cccgggatac
    59401 tgggagaaat tgccgcagtg ctggggaggc atgatgtcag catagtgtcg gttacacagg
    59461 atatcaagga cgaagataat gtatccattg tatttattac tcatgaagcc ctggagggaa
    59521 atgttgccga atcaattaag gaaatcagta tgcttgacag ggttaacaat ttggagaatt
    59581 taataaggat agaaaatttt aattagtcgc taataaataa aaccttgatt ttttcaaggt
    59641 tttcttcaaa tggagctgga gatgggactc gaacccacga cctgagcatt acgaatgctc
    59701 tgctctaccg actgagccac tccagcattt attaacattt gtattctatt tcagattccg
    59761 tttcggcttc tggggcatgt caatcagaaa ctcggtaatc aaaaaacctg caattatcgg
    59821 cagagaaata tagaaccaca aatcagattt ctttacaagc ttcagtatga atgcttcgac
    59881 aagaataact ccaccccata tgagagattt tttcgtggtt ttatccatat tgtacctcct
    59941 gctgctattg atatgaatca aagcaagcca ctttaatatt ctatcactat tataacaaaa
    60001 atgtaagtaa ggtacacatt aaaattaaac gctaactaca tttttttatt ttgttgaagt
    60061 attaatattt ctaattcaaa ttaaaaccac ataataaaag gaattattgg gaataaaata
    60121 gtcagatgcg ttataattga tacatgggct agaaccggca aaaccatata gaatggagtg
    60181 ataaaattga aatataaata caaaggctcc ctgagcctga tcatgattgc ggcattcatt
    60241 atttgcgcgg ccgccggaat ggtgtcctgt gccccagccc caactccaac tccgactccg
    60301 actccaaccc cggctccgga acctgaaccc gcgccggagg aagagtcatt aatcggcttt
    60361 tatccgctga aggagggcaa tatgtgggaa tatgaagggg aaggaatgga atatgcctct
    60421 tttattcaaa aggtaatatt cagctcggga aacaggcatc aggttaccat tgacaatgga
    60481 ggcaccataa tcgccaatgt gctggaggaa agcgagggca gcattgtgaa tatttatagg
    60541 tcgggagaag aattcagcgg cgggaattta cttgataaac caagtaatgt aaatataata
    60601 atgctgcaaa caccgctgga gaaagggaac ttctggataa gcgaagaaaa ccactatgag
    60661 ataataaata cggaagctga tgtagttgtt cccgcaggag aattcaaagg ctgtatagca
    60721 gttaaagtta ctttcaagga ccagacttca aacatgacat actattataa gcggggaata
    60781 ggtatggttc agtccgagtt caggacggat tcaggtgacc tgattacgtc taaattgaaa
    60841 agatatgagc ttaagtaaga taaagaagac tcctacattg aggggtcttc ctctttaatt
    60901 actgctttat ctacgctctt gatttttgaa agctgtacca gcagtactcc ggaaagtata
    60961 atagctgtag caagcaatac cgtaggagtg aagggttcct tcaggatcag agtaccgagc
    61021 aagacagcca caggcgggtt tatatatgca taagttcccg cttttgcagc aggccatttt
    61081 cccaatatat aaatgtatga ggaataacca agcagggagc cgaagacaat aagataaatc
    61141 aatgcgccga ttccttttaa tgagatactt accctgggaa gctctcccaa ggatatgccg
    61201 agaatcagca aagcaattcc cccggccagc atttgtatgc ctatatttac aatcattgaa
    61261 ccttcaggtt taaagctttt agaatatact gagcccattg cccagagaaa ggatgcagcc
    61321 agaatcaata tggcgccttt tacatttata ctgccgacac ctgaacttga gagaaccaga
    61381 attacaactc cggagaatcc aatgagcaag ccaagccagc ctttgaattc tattctggga
    61441 cggccgggta taaaaagctc tattaaagcc atgaataaag gagcggtagc aacaagaagc
    61501 gcggcaattc cggagtggac ccattgctct gcccagacaa ccaggccatt gcctccaagc
    61561 agaaggaaca gtcctacgat agactggttt ttgatactgt tcagatttgt ggggaactct
    61621 aaaccccgca ggcgggcaaa aattagcatt agcaaacctg caacaaaaaa ccttattccg
    61681 gcaaaaagct ccggaggaaa tacactgaca ccgattcgga ttgcaagata tgtagatccc
    61741 catattatgc aaactgcaat aaacgctgac ataacctttg attcttcatt tttaaaaaac
    61801 atatgtaaga cctcccaggc acttttagaa ccattataag gcatgggaca gataatgtaa
    61861 atgaaaatga taatagcgat tgacacatga ggagaatgga gctatcatat acatgagcat
    61921 taattgaata ttgtccgcta ggggagctta gagctgagaa agcactttgc ggtttgaata
    61981 cgcataggtg ttgacccttt gaacctgtct ggttaattct aacggaggga agcggttgtg
    62041 attaataaat agtttgaccg taacttttgg ttacggtctt tttatttagt tattcctccc
    62101 gggcaataat aaaatgaggt ggtgtaaagg tctcttattc cggtaatcaa aatttggcgg
    62161 tgatttatct gattaaagtc atctattggg aggtaatatt atgaaaaggg catctacaca
    62221 aatgcttgca gaggcgggag ttatgattgc ggctgcccag atactcagct atgtaaaaat
    62281 atttgaggcc ccttacggag gttcggttac ggcagggagc atgattccaa ttattgtgtt
    62341 ttcgctgaga tggggagtca agtacggact tcttgcaggc actgcctatg gaattttgca
    62401 gttcctgctg ggaggtgcaa tttattccta tcatgtaata tcaatacttt ttgactatgt
    62461 agttgccttc ggcatgcttg gactggcagg gttattccgc agcaacttta aaggggtatt
    62521 taccggtaca tgcctgggcg tggctggaag gttcatctgt catgtgatat caggagtggt
    62581 cgtatgggct tcatatgctc ccgaaggtat ggatccgatg ttttattcaa tagtttacaa
    62641 cggctcctat cttttgccgg agcttataat cactctaatt attgtaggaa tactctataa
    62701 gccgctgata agcaatctgg gcaagagtgc ataaaaaagc aatggagaca tggggacgtt
    62761 tcgcgtgtct cactggaagt gagacagggg aaacgtcccc atgtctcact tatttttatt
    62821 ggggtaatta gcattttctg ctataataat attgtattta ttttttaaaa taaggatgga
    62881 taagtaatga aaaagatgtt tgcagttttg gctgcactta tcatggtgct ttccggctgc
    62941 agcggagaga ctccgggaaa cggaaccgaa ccggatgata tcgatataaa gaatgaaaga
    63001 cttgtgaaag aatttacggg gacagtaaat atatttcaca gatcctctga caacctgctt
    63061 ctcagtgcag aggagggtga tctttttaag tattatgaaa tagatatagg tactgcagaa
    63121 gtcagggaaa gtaaattgac tgagatggcg gacaggtact tttattatga acccattgga
    63181 aacaaggggt tcatagcagt ggaggataca gactacaaaa acacattgaa attcatagaa
    63241 aaggacggca cggagcatgt tattgcagag gatatggggc cggctgatgc cataaacatc
    63301 tcaatatctc caaacgcagg cagtgttgct tatacatctc ttctggaggg caatgatata
    63361 tacggaatat atgtgtatga tacagaaacc tttgagaaca aaagactcat taatataaag
    63421 agcgatggat tcatagaagg ttttcactat ctggtcaatt ggtctccgga tgagaataac
    63481 atcattatta atgacaaata catatatgat gctttcagcg gatcaaaaaa gggagaattg
    63541 aaatccgcat attcgcagtg gtcacctaca ggttccaaaa ttgcttttgt attggaagac
    63601 gaatcgggac agtggcttaa tgctgaggac taccatatgt ttcagggtaa aaaggtgtgt
    63661 gtctatgata ttttcaaggg cagctatgat gaggtatttg aaattaacgg cgatgaatgc
    63721 attttcgggg atataacctg gggagggaaa gacaggtttc ttgcattccc gggtataaaa
    63781 gcaggggaca ccgatatacc ggattggtat atgaggctta attacagctc ggtatatatt
    63841 gtggatttgg aagagggcgg cagcaaaaga ctggaaacaa atgtggatgc cagtgacgga
    63901 acgatgattg agcttgggaa tcttaagttt tccaatcagg gaaacctgct tagctttacg
    63961 gttgggaatt atgaaaaaag cagtctgcat atagtgaata ccgcaaccct tcaggcagaa
    64021 tcatacgata atgctgaata tctgcattgg attgaagggg agtactattt gatttctgca
    64081 aagggggaca ccatgtattt ttgcatggat aactcaatta taggaataga tgaaaatctt
    64141 caggaagatg tcaaatacac atcaaaatca aagctggatg atttttatat gtctgaggat
    64201 ggaagaggaa ttcttatttt tgaattacag gaagatattc atatagcaag gtatgtcgga
    64261 gaataagacg tatagagcgg ggtgttcatg tgtacgataa ggtcaagaga gaaactatgg
    64321 tgaagactta tttcggctgc aatattgagg atattgcaag cattgttata cttacaccgg
    64381 tatggagcct tgagagtttt atcagcaagg cagatgaagt atcagcagag tttgccggct
    64441 ggtataaggg tgtaacctta aagttcaaca gcaagagcat aacagtgata agcagcggaa
    64501 taggagcacc cagatccggt gattgcgctc ttgccttaag ttataccgat tgcgatactg
    64561 tcatattctc aggttctgca ggagcagtca gccagtgctg cagcataggg gatatgctta
    64621 tagtaagcga agctgtcatt ggggagggtt attcacgtta tcacagcgga gacataaaaa
    64681 gagaccgttt tggtgaactg gtgcagggag acaaagaaac ggcgcaaaag cttctgaatg
    64741 cggtattgaa gtatcaaagc aggtatggga ttaattgccg cgaaggcaga gtattctcga
    64801 tagactcatt actgggagag aggaaggata cctttgatta tatgcaggag aagggctgcg
    64861 atggggttga aatggaggta tcggcagtat tcactgcatg cagatgcgca ggtaaaaagg
    64921 cagctgcact gataattatc agtgatctgc ctcttaaata caaaaatctt tttgagggta
    64981 ttatggagat tgatatgaaa cggtataatg agattgagca tgatttaccg gaaatactgc
    65041 tggaggctgc tgcaggtttc cggcaaataa aataaggagg tgcggaagat gaaatttgcc
    65101 aggtatgaaa gtcaaggaat tgtcagttac ggtttggtgg aagacagtac ggtctacagg
    65161 attgaagggg atatttttgg agagtacaag gttacggaag agcggtatat gctttcggat
    65221 gtcaagcttc ttgctccatg tataccttca aagatagtgg cagtaggagt caattacagg
    65281 gatcatgcat cagagatggg gcatgaactg ccaaaggatc cggtgctttt tataaagcct
    65341 tcatcatcag tcatcggccc ggaggaaaaa ataatccggc ccggtatgtc caaaagggtt
    65401 gattatgaag cagagctggc attggtaatc ggaaaacgga cgaaaaacgt tgaaccgtcg
    65461 gaagctttcc aatacgtgct tggtgcaacc tgcttcaatg atgtaaccgc aagggatctg
    65521 cagccaaaag acggacagtg gaccactgca aaatcctttg atacctttgc tcctatgggg
    65581 ccttttatag taacagggat tgactgcaac aatactgaca tagaactcgt gcttaacggc
    65641 gaaacaaagc aaaagtcaaa tacatcatat ttaataaaca aaacccatga attagtaagc
    65701 catgtatcta aaataatgac tttgtatccc ggtgatgtta ttgctaccgg aacaccttcc
    65761 ggagtagggc ccatgtcggc aggtgatgtg gttgaggtca ggctgcaggg tataggcata
    65821 cttagaaatt atgtagaatg aataatccat aaaaataaag tgcagaaatc tgcactttat
    65881 tttttgtacc tctcctgctt tcctgctgct gttccgtatt aactcaagtg catgttaagc
    65941 tgtcaaatgt ctgaaaattg agaacaattg tacacgaaat agatacatta aataaatagt
    66001 gtatgcaaaa tttgcacaaa aacacttttt ttttgaatta taggtgatat aatgaaagta
    66061 taaaattagt tgatgcgtct gattttgtca aaatagagtt atcagataaa gatgggagga
    66121 tttgtatgaa aaagattctt attaccggag ctttgggaca gattggcacg gaacttaccg
    66181 aatatctcag agagcagtat gggaacgata atgttattgc cagcaacaga agcaagcatg
    66241 gtgatgacag tttctttgaa gcaggccctt ttgaactgct taacgttctg gatgcaaagg
    66301 gattggctga agtgtgcagg aaatatggca taaaccagat aatacacctg gcagcaattc
    66361 tttcggcaaa tgctgagaag aatcctcaaa cagcatatga tatcaatatg aatggaacat
    66421 ataatgtgct ggaagtggca agggaactgg gtatagcagt atttaccccc agctcgatag
    66481 cagcttttgg ggctaacacc ccggctgaca atacacccca ggatactata caaaggcctt
    66541 ccacaatgta cggggtaaca aaggttgcag gagaacttct ctgtgactat tacttcaaga
    66601 aatatggcgt ggatacaaga ggggtgagat tcccgggcat aatctcctat gcagctttgc
    66661 cggggggagg cactacagac tacgcagtgc atatttatta tgaagcattg aagaataaga
    66721 aatatgcttc ctatatagga gccggtacat atatggatat gatgtatatg cctgatgcac
    66781 taaaggcagt acatgacctc atggaggcga atcctgacaa gcttttgcac aggaatgcgt
    66841 ttaatgttac tgcaatgagc tttgagcctg aaatgcttgc cgcagaaatc aggaagcata
    66901 tacctgaatt tatattgacc tatgatgttg atcctgtcag acaggcaata gcaaacagct
    66961 ggcctaattc cctggatgac agtgctgcaa gagcggaatg gggatggaat cctgaatatg
    67021 atttggcgtc gatgacaaag gatatgctgg aaaagctggc agtaaagctg aatattaaag
    67081 aataagtata taaggtatta aggaaagggg aggtaaacag aatgggtttg catgatctgg
    67141 attttataac tcaaaggatt aacgaactta aggagcaggg tgtttacagg aagcttccgg
    67201 tattggagag cccctcaggc ccgagatgca tagttaacgg aaagaatgta atcaaccttt
    67261 cctccaacaa ttatctgggt ttggccaacc atccgagact cagggaagcg gggaaaaagg
    67321 ctattgataa atggggcatg ggagccggtg cggtaagaac aatcataggt acaatgtcca
    67381 tacatgagga actggaagag aaactggcca agttcaagca tgtggaagct gttttggtat
    67441 tccagtcagg ctttacggca aatgccggag caattcccgg gatagtcgat aaaggggatg
    67501 taattatcag cgacgagctg aatcatgctt ccattataga cggctgcagg ctgagcaaag
    67561 ctgacgtagt aagatataag cacagcgaca tggaagatct ggaaagagta ataaaagaag
    67621 ttaaagataa ttacatcata aagcttatta tcactgacgg tgttttcagc atggacggtg
    67681 atatagcaaa gattcccgaa atcgttgaac ttgcggaaaa atatggatgc ctcacttatg
    67741 ttgacgatgc gcattccagc ggagtgcttg ggaaaagcgg tcaaggctct gcgcatcact
    67801 tcgggctgag tgacagggta gatgtacaga tagggactct ttcgaaggct ataggagttg
    67861 taggaggata tgtggcagga agaaggaatc ttatagactg gctgaatcac agaggaaggc
    67921 ctttcctctt cagcactgcg gcaccacctg cagtagcggc agcttgtatt gaagcaataa
    67981 acatactttc agaaagtaca gagcttactg atagaatgtg ggataacgca aatcatttca
    68041 agaaaaagct caatgagctg ggcttcaaca caggaaagag tgaaaccccg attacaccgg
    68101 taataacagg ggacgacaga aaagccgtgg aactgagcaa aaggcttttt gaagaaggag
    68161 tatttgcaca ggcaatagta ttcccgacag ttccgaaggg tggggcaagg gtaaggacaa
    68221 tagtgacagc agcacataca aaggatgatc ttgatgaggc ggcggcagcc tttgcaaagg
    68281 ttggaagaga gttgggactg ataaaataat acagggtaat atcaaggggg cgacgcaaaa
    68341 ctacacggta agcagtcagc ggcgcccctt tttcttatct gaagaatgat gcgacacagc
    68401 tataagtttg gggacatggg gacgtttcgc gtgtctcact gaaagtgaga caggggaaac
    68461 gtccccatgt ccccaaggct tttgctctgg gaatatgact gcaaactgat ggctgaaaca
    68521 tttgccagag gcattacgtc attactaatg atataataat agtatctatt tttatccggg
    68581 ggtgtaaaaa tgagaaaata ctttttaata atattaacag cttttataat tgctgcaatt
    68641 acaggttttt ttgttttccc gcagctttgg acaaccgttc agtacactcc tgacaaagtg
    68701 catcttgttg tcgaagacgg cattgcaggg gatgaggatt cacctatttt ggaaaacggc
    68761 cagctgctgt tctccattga tactataaaa aaattcatcg atccaaatgc ccattgggat
    68821 gaaaaagcca ataaagcggt atttactaca aagaataaag tgctgataat gaagaccggg
    68881 aagctcactg taatggtaaa tgcccgtcct gtggatatta atatacccgt aagacttgtg
    68941 ggcaacaagc cgtatatacc catacagatg ctggacacaa tgtttggaat agaagttcaa
    69001 tggattgagg aaaataatgt tgcggtgctt gattatgcaa aaaataccgt tcagattgca
    69061 gtggttcaga aggacaacag aatcatgagg gcaaagcctg ccttgtggtc cccggcagtc
    69121 agaaaaaata ttgacaaggg tgaagtgcta agagtattcg gcggggatga aaagtggtac
    69181 aaggtaagaa gcaaggacgg gtttataggg tatatgcaga agaaatatgt caaaactgac
    69241 ctcattgcct cagaaacgga tatgactgaa tctgccggca gcactgcctg gaaaccggag
    69301 cggggaaaga taaacatgac gtgggaatat gtagggaaca ggaatccgga tgtatcacag
    69361 ctgaaaaata tcagcggcct tgatgtgata tcccccacct ggttctatat tgtggacaag
    69421 cagggaacgg tagcaaataa ggctgatatc ggctatgtgg actgggccca caaaaacggc
    69481 cataaagtgt gggcccttgt aggaaacgga tccgatccgg atattactca cgattttctg
    69541 aacaataccg acacaaggga aaaaatatat cagcagctgc tgatatatgc caagctttat
    69601 aagcttgacg ggattaacat tgattttgaa aacatatata tgaaagacag ggatatgctt
    69661 actcaattca tgagagagct ggcacctttg ttaagggaac aggggctggt agtgtcgatt
    69721 gatgtcacct tcaggtcttc cagtgagaat tggtccatgt gctatgacag aaaagccctg
    69781 tccaaggtgg ctgattatgt cgcagtaatg gcatatgatc agcattgggc tgcaagtccg
    69841 atagcaggct ctgttgcaga gcttggatgg gttgaaaggg gccttgaaag aattcttgaa
    69901 gaggttccgg gggaaaagct gcttttggga cttcccttct atacaagggt atggaaggaa
    69961 gaaactatag atggtaagat caaggtatcg tcaacagcag tatccatggg gaaggtggaa
    70021 caaatactgt ctgaaaagaa gccggaagta atatgggatg aagaaagcgg ccagtactat
    70081 gccgagtata aggaaggcaa ggccacatat aaaatttgga tagaggatga caagtcaata
    70141 aatctcaagt catctctaat ccacaagtat gacctggcgg gggcggcgtc ctggagaaag
    70201 ggctttgaat cggaggatat ctgggctgtg cttgaggaca acctgaaggt gaagcagaat
    70261 tatgcccaat gggctaatgc caatagtaca gctgctgatt aatttgcaaa ggctgatgtt
    70321 gcatgactat gggaaatgaa ataagaatat cggtaagaga tttggtggag tttgtttacc
    70381 gctcagggga tctggattcg cggtttgcag gaatgggaaa agccattgaa ggaaccagac
    70441 tgcaccagaa aatacaaggt ttgagaaaag atgaggcggc aggcagcgga gaaatctacc
    70501 tcaaggaaat aagtgtttcc atgaggatat gtgtcagggg cattaccttt attatttccg
    70561 gaagaattga cggattgctg caaggcagtg aaggcattac cctggaggaa ataaagacaa
    70621 cttccagccc gttggatatt ataggcgaag acacttaccc ggtgcattgg gcacaggtaa
    70681 tgtgctatgc ttacatgtat gcatcggagc aagccctaaa aacggtcaat gtccgcctga
    70741 cctattataa tgccgacagc ggcgagcaaa aggcttttat caagagggcc gatatcgagg
    70801 aactggaaaa acattttacc gaagcggtgg agaagtatta tgattgggct tatacagcct
    70861 gcgaatggat tgcattgaga aacagcacca taagggatat gggattccca tatgacaaat
    70921 accggaagaa tcagagaaag cttgcagttg cagtatataa gacaataact gcttcgaaga
    70981 ggattttcat tcaggcccct acaggaacgg gaaagacaat ctcggtgctt tttcctgcag
    71041 ttaaggcttt gggagaaggc aataccgata agatattcta tctgactgca aaaaccataa
    71101 ccaggcaggc agccttggat gcaatcagca agatgaagtg caaggaactc agaatcaaag
    71161 ccgttgtgct gacggcaaag gataagatat gcttcaagga ggaatccgac tgcaaccccg
    71221 agtactgcga atatgcaaag ggctattacg ataaggtgaa tgcagtattg aaggaggttt
    71281 ttgaaaagga ggacataatt actagggaac tggtggagga gtatgccgga aggcatatgg
    71341 tgtgcccctt tgagctttcg ttggacttat cctattggac ggactgcatt atatgcgact
    71401 acaattatgt ttttgacccc agggtaagcc tcagaaggtt tttcacggaa acagaaagca
    71461 attacgtgct gctgatagac gaagcgcaca atcttgtgga ccgtgcaaga gaaatgttct
    71521 ctgcggagct ttataagaag cctttccttg aagcaaagcg cttgatgaag gtaaaagcac
    71581 ctttgatatc aaaggcagct aatggtatca actcagcttt gcttaaaata aggaagaaaa
    71641 gcggaagcaa gaactgctgc atggcaaaag aggagctgaa agaactgtgc caggccctta
    71701 gggcttttat cagccatagt gaggaatggc ttggttcaaa tcatggggaa caaggatacc
    71761 gtgaggtact ggagctttat tttgaggcag cggcattctt aaggatttac gagatttttg
    71821 atgaacgata tactgcatat gttgagctgc cgggaagtga tgtaaagcta aagctttttt
    71881 gcctggatcc ttccaagctt cttggggaaa taaccggacg catgaaggct tccatcttct
    71941 actcggctac tttgacgccc ttgtattatt ttaaggatat tctcggaggc agcgaagagg
    72001 attataacat aaggctggat tctcccttcg aggaatccaa ccttagcctg actgttgccg
    72061 gcaatgtgtc caccagatat acggacagag agggcagcct ttcggaaata gccgattaca
    72121 tcaagactgt agtaagtgca agaaaaggca attatctcat atttttccct tcatacaagt
    72181 atatgaatga ggtgtttggc ctatttaccg agagatatcc ggaaatcgga acaattttgc
    72241 agcagcctgc catgcaggag gaagaaaagg agcgctttct tcaatgcttc tgccgggata
    72301 atgaaaagga actggttgga tttgcagtga tgggaggaat tttttcggaa ggaatagatc
    72361 ttgaggggga aaggcttatt ggggctgtca ttgtgggtgt agggcttccg caggtatgct
    72421 tcgaaagaga tattatcatg gatttcttta aaaagaagaa caatatgggt tttgaatatg
    72481 catatatgta tccggggatg aataaagtga tgcaggcagc cgggagggtc atcaggtctg
    72541 aatctgaccg gggcgttgtg ctgcttatag atgaaaggtt ttgcagcagc cgatataccg
    72601 gtatctttcc ccgggaatgg ctgcataata ataatgtaga aagccatgag gaattaagac
    72661 gaatattgga ggagttttgg agtcggacgg agtaatatat gatataataa ttatattatt
    72721 attgctgaca gttttaggag taattaaaaa tgagtgcgat ttcagctgaa aatatgaaaa
    72781 gcttctcaga cactgtatca gatctatata ctctgttctt caggagctat ccctttatga
    72841 agccttttct catgaatata ttcctgttca gctccggcaa ctttggaata atcatcaagg
    72901 aagaaggccg cgaggcaggc aggttttcag tttaccacag tgccgaaggc gttattgtca
    72961 aagaaggaat caaagagggg atcaatgtaa gaatcacatt gaacagaaag cactatgagg
    73021 atatgatgca gaacaggaaa aagtatatgg aaaagcccta taagctgctt aagtttttcc
    73081 cggccttttt aaggtctgta gcaattgatg acagatccct ggcggagaat gtacttgcag
    73141 gaagcagagt aaccttgagg cccggcttag acagcgacat atattatttg ttaaagtggt
    73201 ataatgacat tgagttgaac aagcttgcag gctggagcaa ttccaaggca actccggaca
    73261 agttgaggta caatatctca agaagctttg ggtatgatcc tatgaacctt atgattgata
    73321 atgaagaggg aacgcctata gggacaatac agctctatga tttcaatgaa caggataaaa
    73381 gctgcaagct tggcataaga atcggggata gggattattg gagcaaagga tatggggagg
    73441 atgcagtcag gacaattctt aagtatgcat tcttcaatat tgatatattc agagttacat
    73501 tgaaggtata tgagtacaat gagagggcag tcaaatgcta tctcaaatgc ggcttccgct
    73561 gcgagggcag aacaaggcag tcggcatgca tagacgggaa gttctatgat gaaattataa
    73621 tgggggtact gaaaagcgat tttatccggt tgtcggataa ataagacacg gggggacatg
    73681 gggacgggac acggggacgt ttgggacacg gggacgtttc tcctgtcccg cttttcggga
    73741 caggagaaac gtccccgtgt cccaaaacgt ccccatgccc cccgtgtctt atcacttgtt
    73801 tcccatgtct ttcatatcct gcatcatatc tgatactctg aaataagcat ttctcgctga
    73861 acgtgaaacc tttttgcgag tttttgaact taatgcaggg attaaaacca atcctgccat
    73921 tgcacctaat gctccccaag tcatcatgct tctggaggct cttcgcataa cgatagcact
    73981 tcctttcggt atttatgtaa gcctttgaat gtattgtatt aataggttgc ttttttttgt
    74041 atttattaaa ccatgaaaat aatagtattt ttaaattccg gataaatcga attcgggagt
    74101 atagatgctg tgtgcagatt ccttgccctg cataaacccg ttttccaggc cagcttcaaa
    74161 gaaatagcca agcacttcat catattcgga caagcttatt ctcctgttga tatcattata
    74221 ttgagaggct ttgtgcattg gagtatattg gcacatgatg ctcatgtatg cgtattttcc
    74281 tatattttca cgtacccatt ggaaaattct ttttgaatcc ggtcctaaac cggggagaac
    74341 caggtgcctg ataatcagtc cctttttaat cattccgtct ttgtcaaact cagggtaccc
    74401 tgcctgatta tgcattgctg ttacagcctt tgaggcatac tcaaaataac tgccggtatt
    74461 tgaatatttt accgagtata agtcaccata atacttgatg tcgggaaggt agacatcaac
    74521 agttccctta agaattccga tggtttcttc aagttcataa ccgttggtgt tgtatatgat
    74581 cggcacatgc aggcctttct cttttgcctt tcttacagcc tcgataatat gcagtgcata
    74641 tggagtggga gatacaaggt tgatattatg agcaccctgc tcttgaagct ccagcattat
    74701 ctccgccaat ctttcaattg agattttttt gccggttaat gcaccggagg atgcttgact
    74761 gatgtcatga ttctggcaga acacgcattt catgttgcag ccgctgaaaa acactgtgcc
    74821 ggatcccctg ccgccgctga tgcagggttc ctcccattga tgaaggcagg cctttgcaac
    74881 cagcatttct gatccggcac cgcataagcc tttgccttca tatctgtcgg aaccgcattt
    74941 tctggggcag attctgcaat ttttcaatat atcatacatg gtgattccct ttccgctcca
    75001 cagtttatca gaaggtttca aaccttccga tagaaatatt aaatcatatg gagaagcaaa
    75061 tttgaaggaa ttatttatgc acgtatagaa tactactatg ctgaaaaatt gcaggaaaaa
    75121 tgtaaaatta agcaaaatta acggagcaag ggtgagtttt atgactgaac aggatttgct
    75181 attctctttg gatataggta ccagaaccgt tgtcggcata gtgggattcc atgacggtca
    75241 gaaatacata atcaaggact ttgaagtgga ggagcacaag gaaagggtta tgtatgacgg
    75301 acaagttcat gatattgagc ttgttgcaaa agcagtggaa aaagttaagg aaaggcttga
    75361 agaaaagaca ggccttaagc ttaaaagagt atccgttgct gctgcaggaa ggaccctgaa
    75421 gacttgcaaa ggttttgcgg agctggatgc ggatccgtct gtgggagttg acagggaatt
    75481 aataagcaat cttgaaattg aagcaatcca aaaagctcag aaaagcatgg aggagcagac
    75541 agaagagggg gaacagtcat ttttctgcgt agggtatgcc gtggtgcact attttcttaa
    75601 cgggggtata atcggcaatc ttgaaggaca taaagcaaaa cgcataggag tggaagttct
    75661 ggctactttt cttcccaggc tggtggtgga aagcctgtat gcagtagtcg caagggcggg
    75721 cctggaggtt tcaagcatta ctttggaacc tattgcggcg atgaatgtgt cgatttccag
    75781 cagcttgagg ctgctaaacc ttgctcttgt ggacattgga gcgggaacat cagatattgc
    75841 actgacaaag gacggaactg tatttgcttt tgcaatggcc tctattgcgg gagatgagat
    75901 aaccgaaaag atagcccaag tcttgctgct tgattttgac gaggcggaaa aagccaaaat
    75961 cagcctgaat aatgaaatta tcaaatacac cgatataatg ggactgattc atgaaaagaa
    76021 ctcatcggag gttttgaatg aaatagacga atcaatcagg atacttgctg aagagataag
    76081 taataaaata cttgaattca acgggaaagc gccaagtgcg gtattccttg tagggggagg
    76141 aagccagata ccaaggctta ccggatatat tgccgaatac ctccggatac cttcggaaag
    76201 agtaggggta agaagcaccg atatcataag gaatgtggag atagagagca aagggctttc
    76261 gggaccggaa ttcataacac ctataggaat cgctgttact tcttatttta acaggcaaaa
    76321 ggattttctt catgtaaccg taaatggtgt aaacgtaaag atgcttaata caaaagctct
    76381 taccatagca gacagtctca taatgatgaa ttacaatccc cgcaacctta taggcaggag
    76441 gggaaatccg ctgaccttca aggtcaatgg aagagaggta attgtcaggg gagaaccggg
    76501 agtacctgca gtcatatatc ttaataataa tattgcagga cttgacaggc ccattacaaa
    76561 cggagatgaa ataattgtga agcctgctgt agaaggtaaa ccagcagagg ctaatgtaaa
    76621 ggattattta ggcgattata ttgaaaaaac agtttattta aatgaaaatc cccttaatct
    76681 taggcctatg attactataa acggtcttga atgtaatttg gatgctccta ttaaaaatag
    76741 tgatgaagtg aatatagaaa atattgatac agtacatgag cttttgaaat ctctgaattt
    76801 gaattataat aatatagaag ttgaaatcaa cggcaaaaag gcagagcccg gcgatgtcat
    76861 aaatgacgga gaccatatca gctataaagt gcttgaaaga acagaaacgg agagctttcg
    76921 gaagcctgat aaaaaaacag tctgcgtgaa cgtgactata aacggaaaac cggaagtttt
    76981 agtgacaaac aaaacccaat gcttgttcgt ggatgtattt aacaatattg aaatagattt
    77041 cgacagcata aagggagtaa gtgctatgct tttaaacggg aataaagcta gtttcaccga
    77101 cgtgataaat gacggggacg taattgagct tcactggaag gaaaaatagc aggaatttta
    77161 gttgatatat cattaatggg caatggaaac gtatataata tatcagtaca agtagagatg
    77221 cagttgtaaa cgcagaagag tgggggatgc atatgagcag aagcatgaaa ataggtatcg
    77281 gtgtaattat tgttttattg attgcggctg cagcgctgag tgcggcaatg gtaagcggag
    77341 ataaaatcca ttccaatatt tcaattaacg gaattgatgt gagcggtttt gttccttcac
    77401 aggcggagag catgctgaag aaaaaaatgg agcctattat tgacaatcta agtattgtct
    77461 tgaaatttaa aggaaaagaa tggcagttga aatataggga tattgatttt aaatacgagt
    77521 atgaagatgc ggtgacaaaa gcttatgata tagggcataa gggcagttat ttcaagagaa
    77581 ttaaggatgt ggctgcaact cagctgaatg gagaggatat aaagcttaat tattcctatg
    77641 attcacaata cttgaaaaac agactggagg acatttcaaa agaattggaa caggaagcca
    77701 gggatgcatc aatagtattg aaggagcaag gctttgtaat tactgatgaa gtagagggca
    77761 ggaagcttga tgtcaataaa tcctgcgata ttattaaagc tctgctggat aaagcctcgg
    77821 aaggagaggc ggaattggct gttgatgtta cagaaccgga aattaaaaga tccgaccttg
    77881 aaaatataaa ggacaatatt ggagagtatt caacaaagtt caatgcagct gatgttgaca
    77941 ggactcataa tataaaaatc gccgccagca gtgcatcaca catattggtt aagcccggtg
    78001 aggtcttttc cctgaataag agcatgggcc cacgccttgc aaagttcgga tacaaggaag
    78061 ccgatgtcat aataaataat gagctggttc ccggcatcgg aggaggagta tgccaggttt
    78121 cttccacctt atataatgcc gcactgcttt caaatcttaa aatagtagag cgcaagagcc
    78181 attccctgcc atccagctat atagctttgg gaagagacgc aacactatca gaggattata
    78241 tagatttaaa atttgagaat aacaccaaat atcccattta catttatgga gaagtgaagg
    78301 gaagctgggt taaattcagc atatacggct atgacgaata cccggaaaga actgtcaaga
    78361 taaagacaga gacaattaag gtgactcccc ctcagataag cataatagaa gatcctgctc
    78421 ttccggaggg aacagaggtg gaagaaaaga aggcatatac cgggtatgtt gtcaaaagct
    78481 acagggtggt tatggaaaac ggaaaagagg tttcaaccga ggagcttcca ttaagtgttt
    78541 acagggttgt aaacggggta aaaagagtgg gaaccatgaa ggttgagcct cagccggctg
    78601 aaaatgaagt tttgccggaa gtacccggtg atatataact aaagtgcatt aaagctgtca
    78661 tgacaatagt acttggattg gagaatgttt tatgaacgat tcgctgtatt ccaagaaagc
    78721 tatttgcccc atatgcggca agcagttttt ttcaatgaaa gccaaggtca attcctgtaa
    78781 agttaagaag aaggatgagg atttctgcat ccattacgag gacttgaacc ccatgtatta
    78841 tgaagttttt gtatgtcctt cctgtgcata cgcagccccg gagaattctt tcaaagaact
    78901 gacggaaaaa gaagtcaagc ttcttaagga ggtcttttcg ggaagagaag tgagcagaag
    78961 cttctgtggc caaagaagcc tggatgatgc gacagctgcg tacaagctgg ccattcatac
    79021 tgccgagctg agaaaagcga gtgccagtgt gcttgcggga ttgtgcctta ggcttgcatg
    79081 gctttacaga tttaaggggg acaagcagga gcagctgttc ctggaatatt cgctccggaa
    79141 ctatttggaa gcttatgata aagaaccgct tccaatcggg aatctgaatg agatatcctt
    79201 gatgtatctt ttgggtgagc tgtcaagaag gcttgggaag cttgatgacg ctatcacctg
    79261 gttcgaaaaa gcagcaacaa atcccgaaaa gaaagaaaac cccatgattg agaagctggc
    79321 aagagaacag tgggcattga ctcgggagca gcacaaggaa agtgaaaaat ccgaatagaa
    79381 ataaagctaa aaactgtcca agaggacacg gggaggacac ggggacgttt ctcctgtccc
    79441 gctgaaagcg ggacaggaga aacgtccccg tgtcctccca atgccctcgc gtcgcattat
    79501 tcataatttg tgaaataatt ttaaaaaaaa tttcactttt agttgattga attgtctgaa
    79561 tatattaaat agaatattat tataggcatt tcaatgtatc atggcagttt caggaattgt
    79621 ttatgcatca attttatcgt aaatcagtga ggaatgtatt atgattgaca aggacttgtt
    79681 cataaaaaag attgaggaaa gtgtatcaaa ggttatcgtc ggcaagaagg atgtgttgga
    79741 aaaactattg gtagttttgt ttgcgggagg ccatgtgctt attgaggatg ctcccggagt
    79801 aggcaagact actatggttc atgcgcttgc aagatcaacc ggctgcagct tcaagaggat
    79861 acagttcact cccgacctgc ttccgtcaga tataacaggg gttacggtct acaacataaa
    79921 gcaaggcgaa tttgagttca agcatggacc gataacatca aatattgttt tggcggatga
    79981 aataaacaga ggttctccta aaacacagtc aagccttctt gaggctatgc aggagcggca
    80041 gataacagtt gacggaaata catggaagct gccggagccc ttcatggtgc ttgcaaccca
    80101 gaatcccata gagtatgaag gcacatttcc gctgcctgaa gcacagatag acaggtttgc
    80161 aatcagaata tccatgggtt atcccactta taacgaagaa aaaagcattt tgaacaaatg
    80221 cagttcggat aaagcgccgg agaatataga acctgttatc agcgcggagg aagtactgga
    80281 aattagaaga ctggttgaaa aagtgcatgc ggatgattcc gtacaggatt atattgtaaa
    80341 gctggtccgg ggtacaaggg aacacaagga tgtgtacctt ggctgcagtc caaggggaac
    80401 actggcactt ttcagcaatt gccgtgcact cgcctatatc cggggaaggg aatacgtgct
    80461 gcctgatgat gtaaaggagc ttgtattctg cactcttgcc cataggatca tattgaaatc
    80521 cgaagctgca atacagggta aggatcaata ctccgtactt gcggaaatct taaaaagtat
    80581 taaagtaccg gtggcgaaca ttaatggttg agatgaatag aaaagccctt gcattgattc
    80641 tgctcatttt tgctgcagca ctttttgcag gaggcatatt tatgtatgga atctttctga
    80701 cagctttatt tctggcaata atatcctatg cctccggcag gtacatatat aataaccttg
    80761 ttaatctcac atgggaaata agcagtaatg cagtaagggg ggacagggta agccttaaga
    80821 ccgattggta taatgcaggc attataccaa ttccttattt gaaggttata gccggccttc
    80881 ccaaaagact tgccggtgaa gaacaaaaac agagggtgta ttccataatg cccgaaatga
    80941 taaccactat gtccagagat tttgaatgca ggcataaggg cgtttataaa attggaataa
    81001 ttgaaactgt attcgaggat acattgggca tattcaagtg gaaaaaggtt ttttacgacg
    81061 acagatatct tgttgtatat cccaaggtgc atttattgaa gcatctggac atccctatga
    81121 gacagcagta cggtacttca gctgtcaagc atgctgcata cgaggactgc accggcacca
    81181 aggatataag gaaatacata atcggggaca gcttcaaaaa aatacactgg aaggtaaccg
    81241 cacacagagg agatttttat gtaagaaatg ttgaaatcaa tgcttccgcg gatttaatcg
    81301 tatttcttga tttgtacgat tatggatttg atgaggaaac agcttatgac attgaagaaa
    81361 aaggagcgga atgtgctgca tcaattataa gatatgcttt ggcaaattca atgtccgtga
    81421 atttcacggc aaagggagag gaactggtaa gcctttcggc gaaaagagcc gacaggttta
    81481 atttgttcct tgacgccgta agcaagtttt catcaaaagg ggatataccc attggggacc
    81541 tggtcagaaa agaagcaaga aaactggact gggaagctac tgcagtagtt ataacccctt
    81601 gcatcgaaaa gaatgcttcc gcatttctct ctttgaaagc ttcagggaca gagcttgtaa
    81661 taatatacct gtgcggagac ggcgctaatg gcggaaagga cgcagaaata ctgaagagca
    81721 gcggccttaa cgtgtttgcg gtcggtttgg aggatgatat aaatcaggta ctgggagggc
    81781 attatgaata acaggaatat aaaagaaata ctgttctatt tcagcctgat atttatgatg
    81841 gttttcagct ttaacctggc acttaaagat tttgtcggga tagatatgca tttgattgga
    81901 gtgtttttga tttctctgtc tgcatcagct tcagcatcga tgctcctttt gtttccggcg
    81961 gctgtttcgg caggtttgat tcccgcagcg gggtttctgc ttttttactg ctataaggag
    82021 cctgcatcgg tgaggcttta tttaaaagag gcacaggagt ttacaacatg gctcctgggg
    82081 tatctggcgg gttacaatta ctttgaaaag gcatattcct tgctgtttat catagtcatt
    82141 acacttctgg ccgcattgat tacttcaact gccgtatata aggggaaagg agtccttccg
    82201 cttattcttg cgggtactgc agtaatggcc tacttttggt ttgtatatgt cgaatccgcc
    82261 aggttgtatc ttgcattatt cctgtttgca tccataatgc tttattcata tagaacattt
    82321 aagaagaggc tccgggaatg gatagcggca ggctgtatta tcgattacaa agcagggcgg
    82381 aactggatga tttgttccgg agttgtaatt gcagcggctt tattgttttc tgcagctctg
    82441 cccttaaaaa ttggccctgt aaggtggcag tggcttaatg ataaagccgt gagcctgttc
    82501 ccctttatta ctgaatggag gaacgacgta ctggagtcat acagcttcag ctccgcagga
    82561 tattcggaca ataagctggg cggggaaata cgctgggatg attcagtggt tatgcttgta
    82621 aggacacggg gggaagaagc gctttatctc aggggaaccg ttatggacag atattatgag
    82681 aacagctgga acaagtcggg aaagaggtat aaagagtaca aaccgggcca tttaatgcct
    82741 attccatacg gaaaagatat aaaggtttat acaagggatc tggagatatc acacaggaag
    82801 cttctgactt ctaccatatt tgccccttat tcattatatc ggatacagca cggcgggaag
    82861 aggacatata tggatgaaga ttcggaggca tatgcttcca aaattgccgc aacagatacg
    82921 tcatacaggg tgacaagcat aatcccttac attgatgttg cgaagctgag gttgtccggg
    82981 gcagagactc tggaggaaga tgatttaagg cgctatactg ccttgccgga agatattccc
    83041 gagagggtaa ggtcattatc ccatagaata acaaatgcat atagcagcaa ttatgacaag
    83101 gctaaagcca tagagaaata tctgcgccaa aattatatgt attctctcaa accgccgcag
    83161 ctgccttcaa ataaagaatt cactgactat tttctttttg aaggaaaaga aggctactgc
    83221 acatactttg caacctccat ggcggtactg ctgagggctt cgggaatacc ctgcaggtat
    83281 gtggaaggat tcatagcaag ctatgaagga tctgaagaaa gagaagtaag gggtaccgat
    83341 gcacacgcct gggtggaggt tttctttgac ggatacgggt gggtaacctt tgaacctacc
    83401 ccgcaatatc cggtggtcga aattcttgag cccgggagtg taaaggatga ggtatttgag
    83461 ggagctgcca atgacacagt tacagtggaa aataaaagag ccggtatgcc cggatacttt
    83521 gggcagcctg agggtgcgga aagggtcgga aacggtacaa ttagcggtga tataaaaaaa
    83581 tcatacaatt ttgcaaaaat aatattactg atcgctttgt cagctctaac cgcaagagta
    83641 atatttttgt atgtaaagca ggcaataacg gaaattggcc ttggacgcag cagcgggaaa
    83701 aggtttgctg cagattatat agaggatatt aacagatatt tgagacgtgc cggatttgag
    83761 atgaaagggt cggaaacgct cagggaattt atgaacagag tgggattaag tttcagggaa
    83821 aatcccggcc atatttcaaa gattataacg atattggaaa aaaccagata cggcggttat
    83881 gagccttgtg cgaatgagag aagcattctc aaggttttca gaaaagaagt gaaggccttt
    83941 gcaataaaaa aagccggtat tttacgctat tttataagca tgtatgtcct tggagacaga
    84001 aaaaatgcag tacaaaaatt tcatgttgaa ttcaaaaaaa ttacatgata taataattcc
    84061 gttagcgtga gtggcggaac tggcagacgc actagattca ggttctagcg gtcgcaaggc
    84121 tgtgcgggtt caaatcccgc ctcgcgcacc aaatatgatg gtaaagagct atagcaaagg
    84181 ttatagctct ttttgtttta ttttatagag atataatgct aatttccaat tgtttgggaa
    84241 gtttgtacat tttgaagttt taattttata gatgtataat aaatatgtac ataattattt
    84301 ttcaggaggt tgttagttaa atggggtccg tgagggtaat gccggatagt acatgcgatt
    84361 tgtctccgga gcttattgga aagcatggca tttcggttgt gccgctttat gttattttca
    84421 acggggattc atttagggat ggaatagaca taaaaacaga tgaactgtat aaaatgacag
    84481 aaaggctgaa tacactaact gtccggaatg cccgcattac agcacaggaa tttcctgagg
    84541 gaagaataga agtcatagat tcaagaaacc tttccgccgg gatagggtta ctggttatga
    84601 aagcagttga ttttgccgga caaggccttg gaatatctga aattgaagag ggcaagatga
    84661 ttcttgaaca aaaaataagg ggtaaaaaag aaaaggcatt aaaaagcatg ccggagaata
    84721 ccttgacaga taagagtatt atcgattccc ggagtgtttt tgtcacccat tcaatggcgg
    84781 atgaagaggc tgagtatctt aaaggtgtac ttgaacaaaa gctggaaggg cccgaaataa
    84841 taattaccaa cgcgggatgt gtgatttcaa gccactgcgg ccggggaacc gcaggtatac
    84901 tttatatgga gagttcagaa gcataaatac ttaagagcaa atgcggagga aaatgatgaa
    84961 cagagacagt ttgaaatata aaatgctggt aaccgatatg gattatacgc ttctcaacaa
    85021 ggaaaagaga gtatctgaca gaaacaggga agcgttgaga agggcaatgg agaaaggtgt
    85081 gcatatggta gttgcaacag gaaggatcta tacctccgcc agaatatatg caaaattatt
    85141 gggactggat acaccgatta ttgccagtaa tggcgcgttg ataaaagacg cctccaagac
    85201 tattttcagg gatattttga gtcaggatac tgtaagggag atgctgagat tatgccacaa
    85261 gtacggaatc tattgccact ttttcacaga aaacacgatt tactcggaaa agcttataaa
    85321 tgtatcattg cgttatacag agtggaataa gtacatgggc gaagaagacc aggttaaaat
    85381 aagaattgtt gatgacggag aggaaattat tgaggcggct aaatccgaag tattaaaagc
    85441 tgttgtgttt gatgacgatg atgagaagat acaaaagcta agggatggta ttatggaaac
    85501 cggaatagta tctgtaagcc aatcaatgaa acacaatctg gaagtgatga acaaaggcgt
    85561 taccaaagga aatgccgtca ggatacttgc acagctgtac ggaataaacc gggaagaggt
    85621 tattgcaatc ggcgataatg aaaatgatat cagcatgatt gaatatgccg gccttggaat
    85681 agcaatggga aatgcggaag aatgcttgaa gagggctgct gatcatgtta caggggatta
    85741 tcaggaggat ggagtggcgg aggctataga aaagtttata ttatgattca aattttcaaa
    85801 atatttgttt tacgaaggct caatattatg ttaacataat gttgagggta agcaaatact
    85861 ttgaaaggga agaaacagca tgaaaaagct ttttcttttt atcgctgcag tattggcaat
    85921 gggatacatg agtttcatca gtatgagcat tcctgctgtt ttggagaaaa agagcattga
    85981 gtatgggaat gacggcagtg ctgccaatac catgccggaa ataagagtga tgacgtacaa
    86041 tattcacagg gggataaata aagagaataa gctggacttg aacggaatat cagaagccat
    86101 aagggattcg ggagcagaga taattgccct tcaggaggtg gaaaggttct cggtgaggac
    86161 cggattccgg gatcagatca aatacattgc agacaagctt tcaatgaaat atgcctatgg
    86221 aaaaagcatt aacatattga acggccaata cggaaatgcg atattgagta gatttcccat
    86281 tgaggagtat gaagtatgca aattgccttc cgatggggag agaaggacaa ttttaagggc
    86341 ggtgctcaat gtatacggaa acaggataca tttttacagc actcatcttg gacttgatca
    86401 ggaagaaaga gatattcagg ctgaagagat tgtaaggttg gctgaagaag acaggaactc
    86461 catagtttca ggggatttta attcaaaagc ggacaagctt gggatgctca ccgaaagata
    86521 catggactgt gcaagctatg aaaatgatga tattagggtg acctttgaag gggaagggct
    86581 gagtgagcgt atagattaca tattcgtttc aagaaatttc agggtaaagt catatgaagt
    86641 gcttaaatcg gatgcatcag accactatcc tgtggtctgc acattggatt tcatagattg
    86701 acgcaagtca tttttttttt gcagataata agtatgcggc aaaggctgcg catatttcga
    86761 ggaaagaatt tgatgctatg ccctttaatc caaaacgggt tttattcgaa caggatgctt
    86821 tgaaataccc attggggcgg gaactatttg attactttaa aggcagggat gttcaaacag
    86881 gtatgttgaa gtctcataac agagtcacgg atattccggg caagacaccg gcgcaggcgt
    86941 attccgaagg caagaacaca cttgtagtcg gtgtcagaag gaccttgaat tttgaaagct
    87001 gcaagccttc tgcccattac cagcttcctc tcgcaacagg ttgtatgggt atgtgcatgt
    87061 attgctatct gaacacacag ttgggtaaga agccttatat gcgggtatat gtgaatgtgg
    87121 aaggaatact tgaggctgtg cataacgggc ataccactgt aaggttcagt ctaaacgctg
    87181 caaatgtaat taagaaatac gagcacagaa cgccggaagc ggaggaaagg gtgtcagcgg
    87241 cgagaaaaat tgccgggcat gactatccct tagggtttat aatagctccc gtcttgcttt
    87301 atgagggatg gaagaaggat tacggagagc tgctcaatat gctgaaagac aagcttgata
    87361 cgtacataaa caggagcata tgcttcgaaa taatttcaca caggtttact tccagagcaa
    87421 agaaaaatat aagcgaaata tttcccggca atgacctgcc gatgaatgag gaagaaaggc
    87481 gcttcaaata cggccaattc ggctatggca aatatgtata taatagagag caactttcgg
    87541 atatggagag ctttttcagg gaaagaatcg cagaaatttt tcctaacagc aggattgatt
    87601 atatcatatg agtgtatcaa aattacaaaa ttatgatata attgaaatgt attcatgtat
    87661 gtacaaatta tggtacatgc aaataattat tgtgggggta tgattatgaa caggtctttc
    87721 aaaagaataa gctggctgct tactgttgtg atgattctgg ctatgcttct tccaatgggc
    87781 ggcgtatggg cggatgagag cggtgcggca gctgccggca gtgtgaactt aactatagtt
    87841 catactaacg acacacactc cagagtgctt tcaagcagca gtgaaatcgg ttttgcaaag
    87901 attgcatcca aggtcaagga gataaggagt tccaatgaga atgttctggt gcttgatgca
    87961 ggagatacat tccatgggca gaccatttcc tcacttgtcg agggtgaaag tgttgtcgaa
    88021 gtcatgaata taataaagta cgatgccatg gtcccgggaa atcatgattt caactacgga
    88081 caggaaaggc ttcttgaact tagtcaaatg acaaacttcc ctatagttgc tgcaaatatt
    88141 accaaggcgg acggcagtgc ttttctgtct ccttatatta ttaaggagtt caaaggggta
    88201 aaggtaggaa tattcggcct ggctactccg gaaacaacat acaagacaaa ccccaataat
    88261 gtaaagggct tgacttttga agatccgacg gctgcagcag aaagaatggt tcaggaactg
    88321 gaaggaaagg ccgatataat tatcgctctt gcacatcttg gattggatga gtcctctgaa
    88381 tatacaagca agcttgtagc tgaaaaggtt gacggcattg acctgattat tgacgggcac
    88441 agccatacgg ttctgacgga aggcctaaag gttggcgata cgcttatagt ccagaccggt
    88501 gaatatgaca agaatttcgg tattgttgaa ttggcttgtg aaaacggcag cgtaacggct
    88561 gcgaaggctt cacttatcac aatagaggca gcggagcaat tgcctgaaga tgctgaagtg
    88621 cttcaggcta ttgaaggaat taaggcggaa caagatgtga taacatcagt ggtaataggc
    88681 aggacagaag ttgaacttga tggcgtaaga gagcatgtca gggcgggaga aacaaatctt
    88741 ggcaacctca taactgaagc aatactgaaa gcaacaggcg cggatgcagt catcacaaat
    88801 ggcggcggta tcagagcttc aatagatacg ggagacataa ccaaggggga agtaattact
    88861 gtactgccct tcgggaacta tgtggtagtt aagaaaatta aaggatctga tatcattgca
    88921 gcacttgagc atggcgtatc gcagtatccc gctcctaacg gaggattccc tcatgttgca
    88981 gggataagat ttgcatttga tccggcggct gctgcaggaa gcaggataac aaaggcggaa
    89041 ataggaggta aggctctgga tccaaatgcg gaatacctgc ttgcaaccaa tgactttatg
    89101 gcagtcggag gagataatta tacaatgttt gcgccgggcc ctgttataac ggaactgccg
    89161 ggtctggatg agattgtaat ggaatacata gccaattacg gtacagcgga tattaaagtt
    89221 gacggaagga tactggcaga ggaaaagaaa gcccaggcag cagctgaata cgaggtaagg
    89281 cttggggatg ttctttggaa gatagctgaa aaattcggat tgacatatga aaaactggcc
    89341 gagtacaaca agctgaaaga tgccaacagg atttatcccg gccagaagct gttgattcct
    89401 gagttctaat cataatacga aactgccggt ttcatttccg gcagtttctt tcttttttga
    89461 cctttaatta ttggcaatga aggaataata gggtattata tattatatag tcaaattgaa
    89521 catatttgga ggatgcatat ttgaatgtag aatatattaa tccttttgtc aatgtatcag
    89581 tcaacctaat gaaaatgata tgcaatgaaa cagctacacg gggaaagata tacataaaag
    89641 actcaacctt ttcctctgat aatgttgtaa taattatagg tgtggcggga gaaatcaagg
    89701 ggcaggtttt ttttagcatg aataacacta cggcgtgcaa tattgcttca accatgatgt
    89761 tcggaatgga agtcaaggaa ttggatgata tggctaagag tgcaattgcc gagcttggga
    89821 atatgattat gggcaatgta tcaacagagt tttttaacaa tggaacaaag attgacatta
    89881 cgcctccgac gatattggtg ggaaaggaaa tagccttttc gacaaaagga ttgaaaacca
    89941 tatgtatacc cctggtattg gaaaaactgg ggagaatcga aatagatgtt gcattgacgg
    90001 agtattaatc attcacagga gttgttagct tgagtttgaa tattgtattg gtagagcctg
    90061 agattcctca aaatacggga aatattgcaa gaacctgtgc ggctacagga agccggcttc
    90121 atcttgtcaa gcccctgggt ttttcggtgg acgataagca tctgaaaaga gcagggttgg
    90181 actattgggg cttggtgaat atttattatt atgactcatt tgcagagctt aagagccggt
    90241 attctgacgg attgttcttt tattcaacca caaaagcaaa gaaaaagtat acggaagtta
    90301 tatataagga aaactgcttc atagtcttcg gcaaggaaac tgctggtttg ccgaagcttc
    90361 ttcttgaaga gaataaggaa ttctgcataa gagttcctat gataaataat gcccgatcac
    90421 tgaatctggc aaattcggta gcaattgttg catatgaggc gttaaggcag atagggtttc
    90481 cgggtatgca ttaaattttc cgcatggagg aatgtttatg ggtataaaga ttataacaga
    90541 ttcaatatgt gatgttccaa aggactatgc cgacagatac ggcataaggg tgatgccctt
    90601 gagggtgaat tttggcaata cctcatatat cgatggtata gatttgacct tggaagagtt
    90661 tttaaaaaag ctggaagggg cagaggttct gcctacaaca tcacaagtaa cgccgggaga
    90721 ctttctcgcg gcctatcggg aggaaacagc cctcgggaat aaggttattt ccattcatgc
    90781 ctcttcacaa ctcagcggta cacatgattc agcggtaata gcaaaggagc aaatgcccgg
    90841 gtcagatata tatgttatag atacaatggg aatcagcctt ggcgccgggt tgttggcgat
    90901 caaagctgcc aggctggctg aggaaggctt tgaagcggaa gaagtagtaa aacaaattga
    90961 ggccagtaag cagaagatga aatacatgtt aatactggat accttaaagt atcttcaaaa
    91021 gggcggaagg ctttcgcttc ctgcttcggt gataggctca atactaagca taaaaccgat
    91081 tcttacagta gtaaacggga aactggagct gcttgaaaag tcaaggggaa tgaaaaaggc
    91141 aatattttct gtgatgaata ggataaaaga aaacggctgg tctttaaacg gcaagacagt
    91201 cggaataaac catattacga accttgaaag cagggatatt ttggaggaac agcttaaaaa
    91261 ggagtttaat attaaagagt ttataagggg agaggccgga acagtgatag ccacacacgg
    91321 aggtcccggt gctgtagcgg tacactttga aatttaggag cggatatgat gaaatctaaa
    91381 gtatattttg caggagtaca ttccaatgca gggcaggaga gtttgcttgt caagctgcgg
    91441 aagctctttg acagagccgg attcggagaa atgatttcac agaacgatat cgtagcggta
    91501 aagattcatt ttggagagaa aggaaatacg gcatatatca ggcctactta tgcaaggcaa
    91561 atagttgata agatcaaaga aaaaggggga aaacccttcc tgacggattc caatacgttg
    91621 tataacggtt caagggcaaa cggagtggat cactatgaga cagcaatata caacggcttt
    91681 gcttatgcag ttgtcaatgc tccgataata attgcagacg gaatactaag caaaaattcc
    91741 gttgatgttg agataaacaa aaagcatttt gacagagtaa agatttccaa agaaattgct
    91801 agtgcatcca agatgatagt gatgtcacat tataaaggcc atgagaatgc cggattcggc
    91861 ggggcaatca agaacctggc catgggttgc gcccccgcag cgggaaaaca gcagcagcat
    91921 tcagtggtaa ggccgaaagt atcacggaga tgcgttggat gcgaaaactg cctgaagtat
    91981 tgtccggcag gtgcaattac aaaagaagga gggcatgcac tgatcgggga aacttgcatc
    92041 ggctgcggcg aatgcatttc catatgtccc atcagggcga taaatcctca atgggatatt
    92101 gaaataccgg tattcattga gcgaatgaca gaatatgctt acggggcagt aaaaaataaa
    92161 aatggaaatg caggatttat taatgtactg atgaatatta ctccgctgtg cgattgttac
    92221 ggcttcagcg gtgcatacat agtacctgat atagggatac ttgcttccaa ggatcctgtt
    92281 gcaatagatg ccgcatccta tgatcttgtg aacgaacaga caggaaacat caacagtagc
    92341 ttgaaatgca atcatggcaa aggggaagac aagttcagag gtttatacgg aaatattgac
    92401 ggaacaagac agttatccta tggagaggaa atcggattag gcagcaggga atatgaactg
    92461 ataaaaattt gacaagagaa taaaattctt atatactaaa tgtataacaa aaacgaatat
    92521 aagccataca gattatttcc atattatata aaagcagaaa aaataaatac tgcgggtgat
    92581 ggttttcggg agagaccagt tttggcgccg aaggggcaag tcataaaaag atgctttacg
    92641 atgaagctct caggcaaaag gaccgattac cggacgcaac tctgaaaagc gtaggcaccg
    92701 gagggtcaac ttgataaatg aggtctttgc tcatctatca aagaatatct caggtattat
    92761 acagaggtag aggcatagtc tctacttctt tttgtttaat tggataaata tccgtaacca
    92821 aaatatttag ttggagggat agggctggac agaaatatta ttgtgctgac aaacaacagt
    92881 ctggtaaggg atgagtatgg caaagacctg gatataatat ttattgatgg ggaatatatt
    92941 gatgtgctga agtcagccag ggatatggtg cacaaaggag caaagctgat aactcatccg
    93001 ttgatgggga gcgtcaagcc gaatgaaact ccgttcagaa gcattattgt tgcacaagga
    93061 gattctctgg acatcgggtc tcttacagtc atggagagca gtatagcttc agcggagaaa
    93121 ttccaaaggg atgtcaaagt accgttatgg aatgacagag tacttaatga ttttagattt
    93181 gtggacttga agctttttga aagtgcttat gacagtatta caaagtaaag ggaggagtat
    93241 aaatgggcaa ggtatatgat gttgttataa taggcggagg acctgcaggc ctttcagcgg
    93301 gattgtacgc atcgagagcc agaatgagtg caatgatagt tgaaaaggct aaatggggcg
    93361 gacaggcagg tactacggaa gaacttgaaa attatccggg ttcaattgaa atgccgacgg
    93421 gacccaagct tatggaaaga atgaaaaacc aggcggaagc cttcggaacc gaacttgtga
    93481 aagacgaagt attaagcatt gatgtatcag ataagataaa gaaaataaag ctgggaagcg
    93541 gtaatgaaat aagcgcaaga acaattataa ttgcaacagg cgctcagccg atgatgctgg
    93601 ggatcccggg agaggcggag cttcggggaa aaggtgtatc ctactgtgca acatgcgatg
    93661 ccgatttctt cgccgaattg gaagttgtgg ttgtcggagg cggagatgcg gcggttgagg
    93721 aagccatata ccttactaag tttgcggaaa aggtcacaat agtacataga agagatcagt
    93781 tcagggctgc aaagtccata gcagagaagg caatgaaaaa cgacaagatt gctataatgt
    93841 gggacacagt accggaagag atttacggcg acgggatagt agagggtatt aagctcagga
    93901 atgtgaagac taatgaagtt acagatttca ggactgacgg tgtattcatg ttcgtaggca
    93961 ccaaaccgat atctgatttt gccaagggcg ttgtagaaat ggatcaaaag gggtatataa
    94021 tggcagatga agaaatgaga acatcagttg agggaatatt cgcagcgggg gacgtaaggg
    94081 tgaaatcact gagacaggtt gttacggcag ctgcagatgg cgcaatagca gcggtcaacg
    94141 ccgaaaaata tattgaaaag aattttgaaa attaaaggag gtgaaaatat gatagagcta
    94201 gacaaggata attttgattc agaagtgctt caagcaacag gtcctgtatt ggttgatttt
    94261 tggggagaaa aatgtgaacc ctgcaaagca ttaatgccgg aggtgcacgc tctggctgaa
    94321 aaatacgggg ataaaatgaa aatgtgcaag cttaatacta ctcagaacag aagactggca
    94381 atcgggcaga aggtattggg acttccaact ttcatcgtat acaaaaatgg ggagaagatt
    94441 aaagaaatca gcggagcaga aaactgcacc cctgaagctg tcgaaaacct tataaaagaa
    94501 aacctttaag aaaagctata ataaactgta tttattgaat tactcaaatt attagggagg
    94561 tgaacgtttt gcgtttagag ttgggtaata ttttcattaa ggatgtacaa ttcggtgatg
    94621 ctaccaaggt tgaaaaggga actctttatg tcaacaaggc tgagcttttg gaggcggtca
    94681 gcggtgaccc cagattgaca aaaattgaga ttgacattgc aaaaccaggt gaagaaacaa
    94741 gaataatgcc tgtaaaagac gtaattgagc ccagagttaa agtagaaggc cccggcggaa
    94801 tattccccgg tatggtcagc aaggtagatg tggtcggctc tggcaaaaca aacgtattaa
    94861 agggtgctgc agttgttact gcaggaaaga ttgtgggatt ccaggaaggc atcatagaca
    94921 tgagcggccc cggagcagag tatacaccat tctcaaagac tcagaacatt gttattgtct
    94981 gcacaccggt ggaaaagctc cagcagcatg agcatgagca tgcagtaaga atggtaggat
    95041 acaaggcagc ggcttatctc ggagaagcag gcagaaaagt agtacctgat gaaataaagg
    95101 tatatgagac tccgacatta atggaaggta tgaaacagta tccgaatctt ccgagagtgg
    95161 catatgtata catgcttcag acacagggat tgcttcatga cacttatgta tacggtgtgg
    95221 atgcaaagaa gattgttcct acgttcatat atccaactga aacaatggat ggagcaattg
    95281 tcagcggaaa ctgcgtatca gcctgcgata agaatccgac atacaatcat ttgaacaatc
    95341 ctattattga ggatctttat gaaaggcatg gcaaggattt caattttgta ggagtaataa
    95401 ttactaatga aaatgttact cttgccgata aggaaaggtc ctccaactgg acggcaaagc
    95461 ttgcagaata tttgaatctt gatgcagtca taatatcaga agaaggtttc ggaaatcctg
    95521 ataccgacct tatgatgaac tgcaagaaga tagctgcaaa gggaatcaag actgttcttg
    95581 ttacagacga atatgcaggc agggacggtg cttcgcaatc ccttgcagat gcagacaaag
    95641 cagcagatgc agtggttacg gcaggtaatg caaatgaaat tatttcactg ccgccaatga
    95701 agaaggttat aggttatctt gaaccggcag atacaattgc aggcggattc gcaggcagcc
    95761 ttaaggcaga cggaagcatt acggctgagc ttcaggtaat aaccggttct acaagtgaaa
    95821 taggctatac caaattaagt gcggtagaat attaattttg aaaggatgaa ttagatgtta
    95881 caggataaga aggttatcat catcggcgac agagatggta ttcctgctcc tgcaatagaa
    95941 gagtgcctaa agacaacagg tgctgaaata gtatttgcag caacggaatg ctttgtctga
    96001 acggctgcag gagcaatgga cctggagaat cagaagagag ttttggattt atcggaaaaa
    96061 tacggcaagg agaatgttgt cgtaatactt ggtacttccg aagctgaatc tgcagggctt
    96121 actgctgaaa cagttacagc aggagatcct acttatgcag gtcctttggc aggagtctcg
    96181 ttggggctcg gagtatacca tatgtttgaa ctgaaggatc aggttgatgc aaatgtatat
    96241 gacgaacagt gcggtatgat ggaaatggtt cttgatgttg atgcaatcat caaagaagta
    96301 agctcaatca gagaacagta cagtaaatat taaggttagt taaatggtat cgggatttat
    96361 ctctatcagc aaatctagag aggagggaaa cttctaaatg attagagttg tacattatat
    96421 aaatcaattc tacggtggta ttggcggtga agaaaaggct gatgtcaaac cggaaataag
    96481 agaaggcgtg gttggtcccg gaatggctct taaagcagcg ttgggaaatg aagccgacat
    96541 aatagcaaca gtaatatgcg gggattcata cttcaatgag aatatggaag aagcttccgc
    96601 agcaattctg gaaatggtta agaagtataa tccggattta ttcattgccg gcccggcttt
    96661 taatgcagga agatacggag tagcctgcgg atcaatctgc aaagcggtca aggagaaatt
    96721 taatattccc gtggtcaccg gtatgtatat tgagaatccg ggagttgata tgttcaagaa
    96781 ggacgtccat atagttgaga cagcaaatgc tgctacggga atgagacaag caattcccgc
    96841 aattgctaag cttgccatga agcttgccaa gaatgagagt atcggtgcgc cgcaagaaga
    96901 gggctatatt ccaagaggtg tcaggaaaaa cgtattcaag tcggaaagag gatccaagag
    96961 ggctgttgac atgctggtag caaagctcaa aggcgaaaag ttcgagacag agtatcctat
    97021 gcctgatttt gacagagttg ctccaaatcc tccgattctg gacattaaaa aagccaaaat
    97081 agctttggta acttcaggcg gtattgtacc gaagggcaat ccggacagga tagaatcttc
    97141 aagtgcttca aaatatggga aatatgattt gacaggtgta aatgacctga ctgcagaaac
    97201 ctatgaaaca gctcacggcg gctacgatcc gacatacgcc aatatagatg ccgacagggt
    97261 gctccctgtg gatgtactga gggatctgga aaaggaaggc gttatcggag agcttcacag
    97321 atactactat gcaactgtag gaaacgggac atcagttgcc aatgcaaaga aatttgctgc
    97381 aacctttgca caggagcttg taaaggatgg agtacaggca gttattctca catctacctg
    97441 aggtacctgt acgcgttgcg gtgcaacgat ggtaaaagaa attgaaagag ttggaatacc
    97501 ggtagtgcat atatgtaccg ttgttccgat ttccttaacc gtaggggcta acagaatagt
    97561 tcctgcaatt gcaataccac atcctctggg gaaccctgcc atgagcaagg aagacgagaa
    97621 agcattaaga aggtccattg ttctgaaagc tcttaaagca cttgagacag aaatccacgg
    97681 acagacggta tttgaaaagt agtaataagg gtggtgcata ccacccttat tttcaccaag
    97741 aaattttgga ggtgtaaaag aatgactgca ccagtagtta aaggagcggc ttatgttctt
    97801 gtccatacgc cggatatgat aatgcattac ggcaccacat gtgcgcttga acatgaagcc
    97861 aatccggaat cggaattctt aaagaaattt cctgagtttg tgagaagtta tgaggaagcg
    97921 gtgaaatatg ctcccaatca ggtatatata gggaatatgc ttcccgacga acttaacaca
    97981 atagaaaaac cttggtataa gaatcttgtc caggatgctg atggagaggg caagatgggt
    98041 gagattattg atcaggatga attatatgtt gcattgaaac ttagcgacgc ttttgatctt
    98101 gttgcattgg aaaagacatt ccttgaagat acaaaggcga agctggcaaa gaagccgatg
    98161 tataccgaag ctgagctggc aaagcttaaa gaaggcgcag agcaatctga aattgacagc
    98221 agagtcaaag aaggagcgga gcctttatat atgggcaaga agctggtcgg ctgcgtaaag
    98281 gctgctcatg ataaggataa gaacctgaca gctcatataa tggtagaaaa ccttgtggtt
    98341 aaggcatcag gggtaatcgc attgaagaac ctgatataca gaaacaatat cccggctgat
    98401 tcaattgagt atgttattga atgctcggag gaagcttgtg gggatatgaa ccagaggggc
    98461 ggcggcaatt ttgcaaaagc tgtttgtgaa gccgcaggtg caacaggtgc caccggttca
    98521 gatacaagag gcttctgtgc ggcgccggcc catgcactta taaatgctgc cgcaatggtg
    98581 aaatcagggg tatacaagag tgtggcgata gttgcgggag gtgcaaccgc aaagctgggt
    98641 atgaattcca gagaccatgt gaataagggt gttccggtat tggaagattg ccttggtggt
    98701 tttgcgatac tacttacgga aaacgacggc attagccctg tactcaggac tgatacagtc
    98761 ggaagacata ccgtaggcac cggatcatcg cctcaggccg tgatggaatc cttaataacc
    98821 aaacctttgg ataaagtagg aatgaagata actgatgtag acaagtactc cgtagaaatg
    98881 caaaatccgg aaataacgga atcggcagga gcaggggatg ttcctctggc aaactataaa
    98941 atgatagcag ccttaggagc gaaaagaggg gacattgaca ggacggaaat gatgtcattc
    99001 atcaaggaac acggtatgcc ggggtttgcg ccaacacagg gtcatattcc ttcgggagtg
    99061 ccgtttgtcg gggctgcgag ggatatgatt atgtcaggcg caattgaaag ggcgatgata
    99121 gtagggaaag gaagcttgtt ccttggcaga ataactaacc agtttgacgg agtatccatt
    99181 ataatggaga aaaatacagg cagggaagat tcagcaggta ctgtttccaa agaagaagtg
    99241 aaggaaatgg tagcagaagc aatgaaagat ttcgcatcct tcttactgaa tggaaggcag
    99301 gggtgatatt gatgtctgat aatcaagtca agtcaatgat tggggaagta ttcaacgaga
    99361 ttgccgacgc tattatgacc ggggaattcg gaaaaagggt aaaagtaggc ctcactactc
    99421 ttggcagtga gcatggtact caggagcttg taaagggtgc agaggcagcc gcaaagaagt
    99481 acggcgactt tgaagttgtg ctgattggcc ccaaggttga tacggagctt agggtagttg
    99541 aaacagacaa tgaagaagag atgcataaga agatggatga gcttctggac aatggagaac
    99601 tggatgctgc ccttactatg cattacagct ttccgatagg cgtttccaca gtgggcaggg
    99661 ttattacccc gggccttggc aaggaactca tacttgccac tactacaggc acttcatcaa
    99721 ctcacagggt tgaggggatg gtcaaaaatg cccttcatgg aataattgcc gcaaaagcat
    99781 tgggcattaa gaatccaaca gtaggaatac ttaacgtgga tggcgccaga caggttgaaa
    99841 gagtactcaa ggaacttaaa agcaacggat atgacatcaa tttttcagaa tccgtaagag
    99901 ctgacggggg tgtggtcatg agaggcaatg acctgctgtc ggccgcagct gatatcatgg
    99961 taacggacac attaaccggc aaccttatga tgaagatatt ttcggcattc acaaccggcg
   100021 gaagctatga atcattgggc tacggctatg gcccgggtgt tggagataat tacggcagaa
   100081 taatcggcat attatccaga gcttcaggag caccggtagt atgcgaaggg ttgagatata
   100141 tggcaagctg cgccaaaggc ggtttgacaa agatagccag ggaagaatat gaaaaagcca
   100201 gaaaagccgg gcttgacagc atattaaagg aactgacggc cgatagtgcc aaagccgcac
   100261 cgaagtcgga agtaaagcag cttgagaaga aaccggtaac aaaatcaata gccggaatag
   100321 atatacttga gcttgaaaat gccgtatctt ctcttgccga agagggaata tactcagaaa
   100381 gcggaatggg ctgcacaggg ccaatagtac ttgtagctga ggaagactac atcaaagcgg
   100441 ttgatgcact tctaaagaat aagcatatta ctgaaaggcc tttggactgc aattgttagt
   100501 tgattacacg tgaaagacac ggggaagaca tgaaggacac ggggacgttt cgggacacgg
   100561 ggacgtttct cctgtcccgc tgaaagcggg acaggagaaa cgtccccgtg tcccgaaacg
   100621 tccccgtgtc ttgcccgtgt cctttttgtc acataaaaaa ttagggtgga aatttttgtt
   100681 ggaaaatact ataatatatt tatattgcaa ttgtaataca attgtaatat aataaacatc
   100741 caattatatt attaaggggg aagattgatg ttaaaaaagg gtttaatact agtattggcg
   100801 attgtgatgg ttatgacaat gtttacaggc tgcgcccagc cggcacaagc accggctgaa
   100861 ccggctcagg aagctgaaca ggctccggca gaagaagcaa aaccggaatt atcctttggt
   100921 atgataacgg acgttggcgg actcggagac aagtctttta atgattcggc ttgggaagga
   100981 ctgcaaaagg caggaaagga actgggagtt gaaccggtta tacttcagtc aacaaagcag
   101041 gaagactatg aagctaacat aggaaatatg gctcaatctg ttgatctttc catagctgtc
   101101 ggtttcctga tggagcaggc tatgatatca ggagctgaac agtatccgga tatgaagttc
   101161 ggaataattg atatggtagt tcctaaacca aatgttatgt cagtcacatt caaggagcat
   101221 gacggctcat tcctggttgg tgttattgcc ggtcttacta caaagtccaa tattgttggt
   101281 tttgtaggag gtatggaggg ccctctgatc gaaaaatttg aagttggttt cagagcaggc
   101341 gttgcttctg ttaatccgga tgcaaaagtt cttgtgaact acacaggtgc tttcgacaat
   101401 cctgacaagg gcaaggaaat tgcaatttca cagttcaagc agaaggctga cgttatatat
   101461 cacgcatccg gtgcttgcgg cataggtgta atacaggcag ctgacgaaaa tggtttctgg
   101521 gcaatcggtg ttgatcagga tcagtcagcg gtatcaaaaa acaataaggt tctctgcagc
   101581 atgattaaga gagttgacgt aggtacatat actatagtta agtcatatgc tgacaataat
   101641 ttcgccggag gaaaggcaat cgagctggga cttaaagaag acggcgtagg cttcagtgat
   101701 ggtggaaaca atgtaccggc tgaggttaag gctgttgcag acaaagctaa agagttagtt
   101761 gttaacggca ctatagttgt tccgtcaaca aaaaaagaat tagaggcatt caagccggta
   101821 gaaataaagt aatcaacatc gtgtagttac atgatgaatc aaagctagat gcgctgctgc
   101881 atctagcttt tgcttcacct ttctgatacc agatgccgga caccggatac ctgattaggg
   101941 cagggctttg gagtgcttca caaattcttg tttaaaatga tataattaag acatgcgaaa
   102001 tgttgcattt tttgagaagg ttttcttatt tgatttgaga ggtgggatat aaattgcctg
   102061 cagtaattga gatgaaaggt ataagcaaat cctttcccgg tatacgggca aatgacaatg
   102121 tcaatctgaa aattgagcag ggggagatac atgtattgct gggtgagaac ggcgccggca
   102181 agtctacgct tatgaatata ctttacggac tttatcagcc tgatgaggga cagatattta
   102241 taaagggaaa gccggtcaag atagtcaatc cgaatgtggc gatttctcat ggtataggta
   102301 tggttcacca gcatttcatg ctcgttcagc ccttcaccgt tacagagaat atcatattgg
   102361 gaaaggaacc aaagaaggga gctgctttgg atattgacaa ggcaataaag gatgtagagg
   102421 aaatttccaa gaaatatgaa ttgaaagtgg acccccgagc tgttgtccag gatatttccg
   102481 taggaatgca gcagagggtt gaaatattga agacccttta caggggagct gaaatactta
   102541 tacttgatga gccgacagcg gtgctgacac cgcaagagat tgaggaatta tgcattattt
   102601 tgaggagcct ggtggaccag ggcaagtcaa taatacttat tactcacaag cttaaggaag
   102661 taatatctat gagcgacaag gtaacaatca tcagaagggg taaggtcatt gacacactga
   102721 atacaaagga tacaaacatt gatgaacttg cagaaaagat ggtgggcaga aaagtgagtc
   102781 tcacggtgga gaagaaggaa gctgaagttt ctgctccgat tctggagatc aaagacctga
   102841 gggtattgaa caacaggaag cttgaggcag ttaaagggat agaccttcag gttcatggcg
   102901 gtgaaatact tggaatcgcc ggagtggacg gcaacggtca gagtgaactt gtacaagcca
   102961 taaccgggct ttcaaaagca gaagcgggag aaattcttct ggccggcaag gatattacaa
   103021 acctaactcc gaaggaggta attgccagcg gagtggggca tataccggag gacaggcata
   103081 agagaggcct ggtactggca tatagcctgg cggagaattc catattgggg agccatcaca
   103141 tgaagcagtt cagacaaggt tttgggctga actaccaaaa aataagggaa tattgcagaa
   103201 ggcttattaa ggaatacgac gtcaggacac cgaatgaacg ggtactggca aaatcccttt
   103261 cgggaggaaa ccagcaaaaa ctgattgttg caagggaaat tgacagagat ccaaagcttt
   103321 gtatagcttc acagcctaca aggggactgg atgtgggggc aatagagttt gtgcacaaaa
   103381 agctaataga gctgcgggac gaaggcaaag ctgtgcttct ggtttccctt gaactggatg
   103441 agataatgga tttatcagac aggatagcag taatgtatga cggaaaaatc gtaggaatcc
   103501 ttaatgcaaa ggatgcaaca gagaagaagc tcggcattat gatggctggc ggaagcgctc
   103561 aggatgctgt atagggggaa tgtgaaatgg aatctggaat aaatgaattt aaagaaggac
   103621 tgttgaatac gttaaaggaa attgcatatc cccttgtcgc aatacttatt tctgttattg
   103681 cggggggtat tctggtttac tcctttggca gtgacccgat aatagcatat actgccatga
   103741 taaaaggaag ccttggggat tggaataaaa ccggagagac tttggttacc gttaccccgc
   103801 ttatcttgac aggcctgtct attgctttcg cattcaaatg caatcttttc aacataggag
   103861 tggaaggcca gtttattatc ggatctatta ctgcagtatg ggcaggatgg gcttttgcgg
   103921 gattgccggc aatactgcat cttcctctga cactgcttgc aggcaccgtt gccggcggga
   103981 tttgggcttc ggttatcggc tggcttaagg ccaaggtggg ctcccatgaa gtaataaatg
   104041 gaataatgat gaattacatt gcaatgtata cgagtaattt tctggtaagg acagtattga
   104101 atgtacccgg caaagcctat tccattgaaa ttgccgatac cgctaagctt tggaggtttt
   104161 ctcaggcttt tgagcagttt aatcattcaa gactgcacat tggaataatc ctttccctga
   104221 tagtagccct gattgcttat tatatactta ataagactgt aagagggtat gaaataagag
   104281 ctgtcggctt caatccgcat gcagccgaat acggcgggat aagcgtcagg aaaaatataa
   104341 tccttgcaat gattgtttca ggtttgtttg cagggctggc aggaagcata atggctacgg
   104401 gagtccaata cagggtaaat aacctgttcg gttttacagg ctatggattg gatggaattg
   104461 ccgtttcgct ggtaggaaat aaccacccca tcggaatcat actctcggca tttcttttcg
   104521 gagtactgca aaagggcggc ccccttatgc agatacaagg gattcctaaa gaggttgtcg
   104581 gtatcattca gggaataatc atactttttg tagctgcaaa ctttgttaag gtagtaatgg
   104641 agaacattaa actgaacaag gacttgaaga gaactgcagt gaaagaggag gtggaataga
   104701 atggagggcg taggcatttt aaccataatt ggcatagtag ttacagccag cataagatcg
   104761 gctactccgc tgatattcgc ctccctggga ggcaacttct ccgaaagatc cggtgttttc
   104821 aatataggcc tcgaaggaat tatgacagtc ggtgctttta gtgcggtatt tttctcatat
   104881 aagacaggtt ccccctgggc agggctttta ggtgccatgg ttgtcggagg cttgttcgga
   104941 ctgcttttgg cgttcatgag catatatttc aaagcaaatc aggttatagt gggtactgcg
   105001 ataaatattc tggcagccag cctcactacc ttcctgctgg ttgaaatctg gggaaggccg
   105061 gggcagacgg acaatgttga atactttaaa gctgtggggc ccttcaatct gtttacttac
   105121 ttggcgtttg tcatggtaat cgtatcatat tacgtaatgt ataagactcc cttcggattg
   105181 agagtccgtg ccgtaggtga acacccaaga gctgccgata cattgggggt caatgtttat
   105241 gtaataaggt acatatgtgt aatgataagc ggtgtacttg caggaatagg cggagcatca
   105301 ctttccatag ggtcaatgag ccttttcaag gagggaatga tagcaggcaa gggcttcatt
   105361 gcactggctg cattgatatt cgggaaatgg aatccgatag gggcggcgtt ggcatgccta
   105421 ttcttcggct ttgctgttgc actggaaaca gttgcgacaa atttgccggt tcccagggag
   105481 tttctgtatg cgcttccata tataatgacg atgtttgcgg tatcaggatt tgtaggaaga
   105541 gcggttggac cggctgcagg cggaattccc tatgaaaaag gtgaaaaatg atattatgat
   105601 aaattatata atgcgcttcg gcgcattatt ccttttattt acagaagcaa ataattatga
   105661 cttgtccctc atatatgcta taatataagt ggatttatgc tatataatag taatagataa
   105721 tttattgcat aggatagtat tatttcattg atggggggtg atgaaatgag aatgggatat
   105781 gatttattaa tagaacaaca gcagaagctg ataatgacgc ctgaactaaa gctggctctg
   105841 aagatacttc aattacctgc agtagagctt gaggaactgt tacagcaaga attggaagat
   105901 aatcccattc ttgaactggc ggacgataac agggatgaaa aatccgaagt acagcaaaaa
   105961 caagataaaa aggaaaagga aaaagaaaaa gaaattgatt ggaaggaata cttccagttt
   106021 caaggcaaga gttatgctga ggagggcttt gacagtgatg aggctgctga actctcctat
   106081 gagaatttca taacctactc atatacattg aaagatcacc ttatttcaca gctgcgggta
   106141 tcccaaataa gcaaaaagct tagagacata ggagagtata ttattgagag tcttgatgaa
   106201 aacggatatc ttaccattac aaaggaagat ctggcagcca ttcttgatgt gaagccggag
   106261 gaggcagagg aagcattaag tgtgatccag acctttgagc cttttggagt aggcgcatca
   106321 aatctgaagg aatgcctttt aatacagctt agaaataaag atatttatga taagaaaatt
   106381 gaagtgatta taattaacca cctggatgat attgcatcca acaggattaa caatatatcg
   106441 aaaaagctct ctataagcat tgaagaagct cagaaataca gcgatattat taagaacctt
   106501 gaaccaaaac ccggccgggc atttgaggag ggtacctcaa caaaatatgt aacacctgac
   106561 gtatatatag aaaaggtagg aaaagaatac gtagtaataa tcaatgacta ttatggttcc
   106621 aagcttatga taaatcaatt ttataaaaag atgctgattt ccgaggataa gtcatctcag
   106681 gcctccgctt tcataagcaa taagctgtcc tctgcaatgt ggctgataaa gagccttgag
   106741 cacagaaaaa acactttata taatgttgtg aaagctatat tgaattatca gatggatttt
   106801 tttgaaaagg gacccatgtt tctgaaaaca atgacactga agaagattgc tgaaatggta
   106861 aatgtacatg agtcaacagt aagcagggca ataaacggca aatatgtgca aacaccccgg
   106921 gggatatttg aaataaagta ctttttcaaa agcggtgttg ataatcagga cggagaagcg
   106981 atttcttctg aaagcatcaa aaaaatgatt aagagttacg taagcggcga ggatgcaagc
   107041 aagcctctaa gcgatcagca tatagcagat ttacttgtga aggaaggatt tatgatttcc
   107101 aggaggacag tggcgaagta cagggatgaa ctgggcatct tgtcttcttc aaaaagaaaa
   107161 agatattaaa ggcggcaatg ataaggccgc ttttttattt tcttgcatac tttactgata
   107221 aattataaat tttaacaaat aatacaatat tatattgaaa ttggaacatg catataatat
   107281 aattaactca atcgggacgt tatcattata tacgggacaa aatatgacca taggggtaat
   107341 gcaatgaacg aatttttgaa tttacagaaa agactggttc cggaaataat agagattaca
   107401 gaaaaaagat atagcattct aagggcaatt gagttcaatc agcctgtagg gagacgcgct
   107461 cttgcgggaa tactcgggca gagcgaacgt tgggtaagga gtgagcttga tatcttgaag
   107521 agccagaatc tgatttatgt gcaggctttg ggcatggtgg taacagatga aggcaatgaa
   107581 gtgataaaca agctcaggga ttacatatca gaacttaagg ggctcaagga ccttgaaaag
   107641 gcagtcaggg aaatgctcaa cataagagaa ttaattgcag tgccggggga tacggaaaat
   107701 gagctgcagg tagttgttga aatggccaaa gcggcttttg aatatatcaa aggaataata
   107761 gaggataatg atataatcgc agtgagcgga ggttattccg cattgtcagt tgccgagaat
   107821 gcacgcagga ttgatgcaca aaacattacc atagttcctg ccagaggcgg aataaaaacc
   107881 gatatggagc gccaggcaaa tattgttgca gagaagttag ccaagaatat cggaggcaat
   107941 tattatcttc tgcatcttcc ggacaatata aatgaagata tgctgaaaag gatggctgat
   108001 gatccggata tagccaaggt gctcaattac atcgctgaaa ctgatgtttt gatatacggc
   108061 ttaagttcct ttgaatccat atccaggaaa agaaatttta ccgaagagca ggttgaagag
   108121 cttaagactt tgaatgtcag ggctgaggca gtaggctgct attttgattc taacggaaat
   108181 attgtatata aatcaggcag cataggtata aaacctgagc atttagatag tataaaaact
   108241 gtaatgtcag ttggaggagg agcccgaaaa gctgaggcta tcgtattgac aagcaagggc
   108301 agaaaaaaca gagtagttgt aactgacgaa gctgctgccc gtgaaatata taagatttta
   108361 aaaggaggaa attaaaatgg cattaagagt aggaatcaac ggttttggaa ggatagggag
   108421 gccggttttt aaaatcgctt cggagaagtt tggagatgat gcagaaatag ttttaataaa
   108481 tgatttaact gatacgaaaa cactggcaca cttattgaaa tatgacagca catacggcag
   108541 gtatgacaga gatgtacagg ctaaagagca ttcattagtc atagacggga aagaaattga
   108601 gatatgtgct gagacaaaac cggaaaacat caggtggggt gactacggag ttgacgtggt
   108661 tatcgaatcc acaggcaggt tcacaagcaa ggaagcggca atggctcatg tggctggggg
   108721 agcaaagaag gtaataatat ctgctcctgc aaagaatgaa gacattacga tagtaatggg
   108781 tgtaaatgaa gacaagtatg atcctgaaaa acatgtggta atatcaaatg cctcctgtac
   108841 aaccaactgt ttggctcctg tagccaaggt gcttcacgaa aacttcggaa ttgtaaaggg
   108901 tttgatgaat acaatacatt cgtatacaaa tgatcagaga atacttgatc tgcctcactc
   108961 agaccttaga agatcccggg ctgccgcatt gtcaataatt ccgacaacta cgggagctgc
   109021 aaaagcagtt gcattggtgc ttccggagct taaaggcaag cttacggggc tttctatgag
   109081 agttcctact cctacagtgt cggtagtaga tttggttgtc gacctggaaa ggaatgtcac
   109141 tgttgaggaa gtgaatgctg ctctcaagag agcggcggaa aatgagctga aggggatact
   109201 tgagtattgt gatgaacctc ttgtatccat agattttaag aaggatccgc attcctcaat
   109261 agttgatgca ctatctacta tggttattca ggataacatg gtaaagatta tctcatggta
   109321 tgataatgag tggggctatt ccaacagagt agttgacttg gtaaaataca tttcaaataa
   109381 gtagaatcga ctgggaggat tcttgttatg aataaaaagt ccatacagga tatagatgtt
   109441 gacggtaaaa aggtgctggt aagagtagac tttaatgtgc ccttagatga aaacggcact
   109501 atcacggacg acagaagaat aagggaagca cttccgacaa tcaggtatct gagggagcat
   109561 aatgcaaaag tcatattgat gtcacatctg ggaaggccaa aggggaagtt caatatgaaa
   109621 tattctctga agccggtggc tgagagattg tccgggaaac ttggagccga agtaaggctc
   109681 gccggtgacg taataggtga ggaagtatcg gcaatggtaa acagcctcaa agaaggggat
   109741 gtaatgctcc ttgaaaacgt caggttctat gaagaggaag aaaagaacga caaagagttt
   109801 tccggaaagc ttgcagcctt gggtgatata tatataaatg atgccttcgg tactgcccac
   109861 agggcacatg ccacaacagc aggcattgca gaatacctgc cctccgcagt aggttttctg
   109921 ataaagaagg agctggaaat aatggggaag gctctgactg caccggaaag gccttttgtt
   109981 gcagtactgg gcggagcaaa ggtctcggat aagatcggcg taatagaaaa cctgatggat
   110041 aaggttgata ctcttttgat tggcggcggt atggcctata ccttcttcgg ggcaatgggt
   110101 tatgagacag gcaagtccat ttttgaagcc gataaagcag aactggcggg tgaactgctg
   110161 aaaaaagcag aaacaaaagg cgtgaaattg ctgcttcccg ttgatactgt cgttgcttca
   110221 gaattcagca atgacgcacc taaaaaaact gtaccgtctg ctatgattcc gaaagatatg
   110281 atgggtatgg atatcgggga ggatacaaga aaaattttct cggaaattgt taagaacgca
   110341 aggacagttc tatggaatgg ccctatgggc gtatttgaaa tgccgaattt tgcaaagggc
   110401 acctttgaat tggcaaaagc tatggctgaa tgtaccggga caacaatcat cggcggcggt
   110461 gactctgccg cggctgttga acagcttggc tttgcggata agatgacgca tatatctaca
   110521 ggaggagggg cttctcttga atttatcgag ggtaaggtgc ttcccgggat agcggcaata
   110581 gagaataagt aatttgggag gtaatattat gcgcagaccg gttatagctg gcaactggaa
   110641 aatgaacaaa acagtaaacg aagcaataaa gcttgcggag gagcttaaac ctcttgttaa
   110701 gggagctgcc gctgaagtag cagtgtgtcc tgcctttatt tgcctgaatg atttggtaaa
   110761 ggttttaaag ggaagcaata taagagttgg tgcgcaaaac atgtaccata aggaaagcgg
   110821 cgcatatacc ggtgagatat ctcccgtgtt cttaaaggat atagaggttg aatatgtaat
   110881 attgggccat tcagaacgca gacagtattt cagggaagag gattcggaaa ttaacaagaa
   110941 ggtaattgct gcactggacc atggattaag gcctattctc tgcgtgggag aaacccttga
   111001 gcagagagaa gcgggggtga ccttcgatgt catagcagct caggtgaaag ggtgtcttga
   111061 aggccttgag aacagaaata cagatgactt gataatagcc tatgagcctg tatgggctat
   111121 aggaaccggc aaaactgcga ccagtcagga tgcaaatgaa gtcattggat tcatcaggaa
   111181 gcagctggcc ctattgcttg ggaatgatgc ggctgacaaa acaaggatac tatacggagg
   111241 cagcgttaag tcctcaaaca taaaggaact tatggatatg cccgaaatag acggggctct
   111301 tgtaggggga gcaagcttgg acgcccaaga gttttcacgg attgtaaatt tcaatcagta
   111361 ataaatatat agatttaagg agggtactga ctatgacagt aattactgac atatacgcaa
   111421 gagaaataat agactccaga gggaatccaa ccgtagaggt tgaagttgag ttggagggag
   111481 gagctttagg cagggctgca gtaccttcag gagcatccac cggggctttt gaagctgtag
   111541 agcttagaga cggcgataag tcaagatacc ttgggaaggg tgtgctgact gcagtgaata
   111601 acgtaaatga aataattgca ccggaaataa taggcatgaa tgcattggat caggttgcaa
   111661 tagaccaggc tcttattgaa ctggatgaca ctcccaataa ggggaagctg ggtgccaacg
   111721 ccatattagg agtatcactg gctgtggctc atgcggctgc agaggcgctg ggacttccgc
   111781 tttatcagta tgtcggcgga gtaaatgcca agactcttcc cgttccaatg atgaatatac
   111841 taaacggcgg aaagcatgca gacaataatg tggatattca ggaattcatg gttatgcctg
   111901 tcggtgcgga aagctttaaa gaagcattga gaatgtgtgc cgaggttttt cataatctaa
   111961 aatccgtgct taaagccaag ggctataaca cggctgtagg agacgaaggg ggttttgctc
   112021 caaaccttac ttccaacgaa gaagcactgc aggttatcat tgaggcgata aagaaggcag
   112081 gctatgagcc cggcaaggat ctttacatag caatggatgc tgctgcaacc gagctttaca
   112141 aggaggacgg caagtatcat ctggatggtg aaggagtagt aaagacttct gaagaaatgg
   112201 tcgattacta tgagaatctt gtcagcaagt atccgataat ctcgctggaa gatggacttt
   112261 ccgaagaaga ctgggacggc tggaagcttc ttaccgaaag gttgggtaaa aaaatacagc
   112321 tggttggcga tgacctgttt gtaacaaata cagagagact ggaaaaaggc atagaaatgg
   112381 gagtagccaa ctccattctc ataaaggtaa accaaatcgg tacactgaca gagacccttg
   112441 atgcaattca aatggcaaac agggcaggtt ataccgcggt tgtatcccat agatcaggcg
   112501 aaacagaaga cgtaacaatt gctgaccttg ttgtggctgt caatgcagga caaataaaaa
   112561 ccggtgctcc ctcaaggact gacagggttg caaagtataa ccagcttatg aggattgaag
   112621 aaggattgga tacaatgtct gaatatttag gcgtaaatgc attttttaat ctataggcca
   112681 tgacgtaaga gcctgataaa aaattctggg gagtcgtatt tttgaattaa aagtgctata
   112741 caatttaaaa atatgtgata taataatggg tgaaattatt tcatcccatt attgtatttt
   112801 atcagccttt gtgttaaaat ggtgtttgta gaatttttcc tgataattat gtgaataata
   112861 agcggaaaga gcgaatgaat ggcaataaat gcacgttctt ttggcacaaa cggatgatct
   112921 ggatgaagat gggaggtgca ggaagtgaga ttggctatag taattttaca gtacgttgtt
   112981 tgtttggtat tgattgtaag tgtgcttctg cagccgggaa ggagtgccgg gctgtccggt
   113041 tcaatagcgg gaggtgcgga acagcttttc ggaaagaaag caagcaaagg ctacgaagcc
   113101 ttgttttcta aactgacaac agcaagtgca attctgttca tggttttatc ggttgtactg
   113161 gtacttgtaa aataatattt caataatgat agcctgactt tttgttaggc tatattatca
   113221 atatttcgtg tatacagtat tacagtatat cacatttatg tattaggagg gttatttgga
   113281 tggatttatt gattctggct ccgctgtcag gagtacttgc tctggttttt gcatggtttt
   113341 tagcaaacag tatcgcaaag aaagaccccg gaaacgacag gatgaaagaa ataatgggct
   113401 ttatccacga aggggcaatg gcatttctat tcagggaata taaggtgctt gttgtattta
   113461 ttattgtatt ggcaataata atcggagtat tcctcaaaag ctggctgaca gcaatatgct
   113521 ttgttgccgg ggcatgctgc tccatacttg caggttattt tggaatgcag gtggcaacaa
   113581 aagcaaatgc cagaacagcg aatgctgcac agagaggcca gaatgaagct ctcgggatag
   113641 cattcagggg cggagctgta atgggcatgt gtgttgtagg tttaggcctt ctgggtttag
   113701 ggactttata tatatttata agagatgcaa gtattgtgac aggttttggc ctgggtgcaa
   113761 gttcaattgc tttgtttgcc cgtgtaggcg gcgggatata tacaaaagct gccgatgttg
   113821 gtgctgatct tgtaggaaag gtggaagccg gcatacctga ggacgacccg aggaatccgg
   113881 cagttatagc tgataatgtc ggcgacaacg taggtgacgt agccggtatg ggagctgact
   113941 tgtttgaatc atatgtgggt tcgattattt ccgcaataac tcttggtgcg atagtatatg
   114001 ccggaaaggt tgaaggagta ttattcccca tgcttttggc tgcaataggc atagtagctt
   114061 cgattatagg aacttctttt gtaaagaccg atgaaaagag taacccgcag agcgctctca
   114121 gcagaggaac ttatgggagt tcgataatcg taattgtggc atcttttata ctaagccgct
   114181 atatgttcgg taatctgaat gccttctggg cagttgctgc agggcttgtc gcaggatcga
   114241 taatcggttt tgtatcagaa gtatatactt cttctgacta taagtatgta aagaaaattg
   114301 ctcaacagtc gggaacaggg tctgctacta caataataag cggtcttgga gtgggaatgc
   114361 tctctacagt ggcaccgata atcttcattt gcgctgcagt gcttgtggca tatgattctg
   114421 ccggcatcta cggtattgct cttgcggcag taggtatgct ttcaacaaca ggcatgacgg
   114481 ttgcagttga cgcgtatggg cctatagctg ataatgccgg aggaattgcg gagatggccg
   114541 gtctgcctga gtcagtgaga gaaattacag ataagcttga ttcagttgga aatacaacag
   114601 ctgcaatagg aaagggcttt gcaataggct cagcggcatt gactgccctg gcgttgtttg
   114661 tatcctattc cgaagctgcc ggccttaaga caatagatat ccttaaccct aaagtaatgg
   114721 ttggaatgtt tatcggaggc gcgctgcctt tcctctttgc cgctctgact atggaagcag
   114781 tcggcaaggc cgctaatcag atgatagaag aagtcagaag gcagtttagg gaaactgccg
   114841 gcataatgga aggcacaacc aagcccgatt acgcaaggtg tgttgatatt tcaactgctg
   114901 cggcattaag agaaatgatt gtaccggggc ttcttgctgt catagttcct atagctaccg
   114961 gctttattct gggcaaaggt gctttggggg gacttattgc aggtgcggtt gttacaggag
   115021 tactcctggc aatcatgatg gcaaattcag ggggagcatg ggataatgcc aagaaatata
   115081 tagaatccgg caagatgggc ggaaaaggca gtgttgcgca caaagctgca gttgtcggag
   115141 atactgtcgg agatcccttc aaggatacgg cagggccttc aatgaatata cttattaagc
   115201 ttatgactat agttgcattg gtatttgcac cattgttcat gtagtttcag catatatccg
   115261 aaccatattt gataatacta ataatatact gtaatattaa agagatggta gaaattatca
   115321 tctcttaatt ttttattgta aagaatatat tttcactgtg gaatatactt atgccggagg
   115381 ttatcatgaa aacattactt atttacctca taaccgtcaa tattgcagcc tttgctgcta
   115441 tgggagcgga taaatacagg gcgcagagga ataaatggag gataagcgag tcaagcattt
   115501 tcgctttagg acttattgga gggggagcag gcatatatct tgggatgaaa ttattccgcc
   115561 acaagaccag gcatcttgaa tttacactgg gaatacctgt aataataatt ctaaatgcaa
   115621 ttgcgttatg gtatattttg caaaaacttg agtaaatgag acacggggag acaaggggag
   115681 acacggggac gtttcgcggg acatggggac gtttcgcgtg tcccactgga agtgggacag
   115741 gggaaacgtc cccatgtccc ggagaaagtc cccgtgtctc cccttgtctc actgaatgtt
   115801 atgaacaaaa tatgttcata ataattacat attgaatccg cgaccttggg ggatgttttt
   115861 cttgaacgat ataaacagca aatatgcaaa agaattctat tttaaaggca ataacacagg
   115921 ggtgctgctt atacatggat ttacgggaac accctatgaa atgcgattct tgggggaatt
   115981 cctgagagat aaaggctgca cagtaagagg aatcctgctg aaaggacatg gtacaacact
   116041 gaaagatatg agaagatgca gctataggga ttggatccat ggagccgtag aaggatacag
   116101 atcgctgaag caggaatgtg atgaggtttt tgcggttgga ttttccatgg gaggattgct
   116161 gtccttatac ctggcaagga actatgatat aagaggtgtt gtaacccttt cggcacctat
   116221 aagggtatat agcagagcag ctgctttggc ttttatagaa agaaacctca gaaatcacat
   116281 gactggaaag actgagaaca gggaaataaa tgtaatcggt tatgagagag cgccattaat
   116341 tagcgtgcat catttgttca agctgataag atatgtgaaa gcaaacctga agtatgtaaa
   116401 aaaaccggtg cttattatgc aatcatacgg tgacagggcg gtaagcccga aaagtgcaaa
   116461 tattatatac ggcagcatag gttcgaagga taagagtata atatatttac acaaaagcgg
   116521 gcatgtaata acctgtgaca aggaaaaaga gcaggttttt aaggaagtat acagttttat
   116581 aaagaataaa tccactttta tcagagaagc ggatagaaaa gaagagaggt gcagcagtgt
   116641 ttaatacaga gaaagaaata aaccaggaaa taaagagcaa attgttagct ttcatgagag
   116701 aggacgcata caaacccttg tctcatgatg agcttattca ggcatttcag gatgattcac
   116761 ttcaggtgcc ggaattggac gaagtgctga attcccttct ggaagaaggg agcataataa
   116821 taacaagaaa aaacaaatat ggcctgacag aaaagatgaa tatggttgtg ggacgcatac
   116881 aagggaataa aaagggtttt gggttcctta ttccggacaa taaggatctt cgtgatttat
   116941 tcatacctgc agaaaatctc aatggagcaa tgaacgatga cagagtaatt gtaaggctta
   117001 cggcaggtga gtcggggact gggaagagag agggagaggt agtaagaata ctgaaaagag
   117061 cgaatgccaa aatcgtaggt accttcgaaa acggcaatgg ctatggattt gtggtgcctg
   117121 atgatcaaag aatatatcag gatgtttttg tatccaaatc tgacttcaat ggtgcgaaag
   117181 acggatataa ggtggttgtc gagataacca ggtggccgga ggcaagaaga aatccggagg
   117241 ggattgttgt tgaggtattg gggcataagg atgatgtagg caccgatatc atatctatta
   117301 tacggggcca caatctgccg gaggagtttc caatcgaggc agagaaccag gccggaaaaa
   117361 tacctgaact ggttagtgat gcggatatag caaaacggag agacctgaga gataaaacaa
   117421 tagttacaat cgatggggaa gacgcaaagg atctggatga tgccgtatca ctggagatac
   117481 ttcctgacgg aaattatatg ctgggagtac atatagcgga tgtaagccag tatgtatttg
   117541 aaaattcaca tttggacaag gaagcactaa aaagaggtac aagcgtgtat ctgattgaca
   117601 gggtggtgcc gatgcttccc aaaaggcttt caaatggtat ttgcagtttg aatcctcaga
   117661 tagaaaggct cactttgagc tgcgatatgg ttatagacaa gaagggcaag gtaaaagaat
   117721 atgagattta tgaaagtgtt ataaagacaa gagaaaggat gacctatgaa aatgtaaata
   117781 agatacttgc cggcagtgat cccatgctta cagaaaggta tgattatctt gcagatacct
   117841 tcaggcagat ggaagagctt atgctgatac tgaataaaaa aagacgttta agaggaagcg
   117901 tagactttga atttgatgaa accaaaatca tattggatga aaagggcagg cctatagaga
   117961 taaagcccta cgaaagagga atatccgaaa ggataatcga ggaatttatg ctggtatgca
   118021 atgagacagt tgcagagcat atgtattgg
//
LOCUS       DAOQUR010000002        93477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_275_length_93477_cov_9.515178, whole genome shotgun sequence.
ACCESSION   DAOQUR010000002 DAOQUR010000000
VERSION     DAOQUR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 93477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 93477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93477
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_93477_cov_9.515178"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKI50_00560"
     CDS             complement(<1..649)
                     /locus_tag="PKI50_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02240.1"
                     /translation="MNKNALNFGYWSAVTAALAFVAYTICFIAILLVNPVFIWTNFET
                     YIKAAQTTNQAFKHIAMLFMILYGACFVIQLCSIEEIAESSKKYFARIAELFGIGFFA
                     LIGINYFIQISAVRMQINSGQTNGLEQFIQANPISGIAAVNMLGWTIFFGLSCIFAAW
                     VFGNTRIERIIKCALLANGIMMFSSAVGYLFDIAVLIFLCMNIGMGAAVLTAEIYL"
     gene            complement(695..898)
                     /locus_tag="PKI50_00565"
     CDS             complement(695..898)
                     /locus_tag="PKI50_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02241.1"
                     /translation="MKKNRIIAVIALLMIFIFLITFVSFLTDAIIPDNSIYYVFMHMV
                     FLVILGICFFPFSEIILSTHKLE"
     gene            complement(961..1326)
                     /locus_tag="PKI50_00570"
     CDS             complement(961..1326)
                     /locus_tag="PKI50_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HNT02242.1"
                     /translation="MKITVVKENDTEIAVVISNEILIKDVQSALDFIAAIDYETGCNH
                     IVLNKSAICEDFFDLKTRFAGEVLQKFINYHIRIAIVGDFSVYSSKSLKDFIYESNKG
                     RNIFFLPDEEQAIDKLGMI"
     gene            complement(1339..1545)
                     /locus_tag="PKI50_00575"
     CDS             complement(1339..1545)
                     /locus_tag="PKI50_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02243.1"
                     /translation="MLVRKADLNDLSNIIDLWYEMMVFHVNVNDIYRLKDNAKTISID
                     NYLAQSFWKKNNYTGYNEICYKYL"
     gene            complement(1577..2254)
                     /locus_tag="PKI50_00580"
     CDS             complement(1577..2254)
                     /locus_tag="PKI50_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNT02244.1"
                     /translation="MSYLIDFKKITLEQYKKELEKRTFIPSRQILKDKADIHFNVFVK
                     ANIKTLEELFSVLKNSKLKAELLNKNKNISDEYLTILLRELKSIQPKPVKLRDFTWIS
                     NNTIDKIEKAGISNTQMLYEKLGKSYEREKFVNTFGIDEHEIIELLKLSDLTRIQWVN
                     TTFARVLFAAGFDTVEKVSKASPEDLYNKVALKNEAMKLYKGKIGLNDMKLCIEAAKC
                     IDIEIEV"
     gene            complement(2403..3140)
                     /locus_tag="PKI50_00585"
     CDS             complement(2403..3140)
                     /locus_tag="PKI50_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT02245.1"
                     /translation="MNELFDMIFKRKSIRRYDYNLSITEAEMEDIRQAINNFRPLVKD
                     IKVKFKIVESKETTSKRGEYCLLMYSEEKPLYLLNVGYMLEQMDLFLASRNIGVCWYA
                     LAKTKELKLDGLEYVIMLAFGKSHPEDFRKDFSKTNRKDLETIWKGEHYSDIGEIVRY
                     APSACNTQPWRVVSEENCLRIYRTTQIKSFMPAGKLSYYNSIDMGIFICFLDIALSQK
                     GYKYERILSTEKCNDSELIDIAAYNIE"
     gene            complement(3320..3769)
                     /locus_tag="PKI50_00590"
     CDS             complement(3320..3769)
                     /locus_tag="PKI50_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNT02246.1"
                     /translation="MSYKLLGVCGLYCGACYHYQASFPRNAHLLRELLKKDKTVEDPT
                     CQGCRSDKLYPHCSKCKIRECAEDKGIEYCGDCIEAPCEMLRNFQLDGRAHHLDIMDN
                     LAELKEQGCEKWLKKQELKWKCQCGAEHSWYEAECHFCGLSFDFYYR"
     gene            complement(3993..6809)
                     /locus_tag="PKI50_00595"
     CDS             complement(3993..6809)
                     /locus_tag="PKI50_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT02247.1"
                     /translation="MDKGAHFYRCDFQVHSPRDINWHGPEAISNEERKAYAETLVQAC
                     RTKGLHAIAITDHHDFVFFPYIKQAALAELNDNGVCYPDEEKLIVFPGLELTLASPPC
                     QALLILDSAFPENLLQGVLTTLGITPNPDTESRTATIVPIPQAVTKNFCELYEKLSNL
                     DYIKGRFTIIPHVSDNGHKTLLRSGFADFYKTMPCVGGYLESSISTLGRGNTDIVNGK
                     NRHYGFKEIALIQTSDNRKATHEDLGRHSTWIKWAEPTAEALRQACLAKNSRISQEIP
                     VLPPVFITSLDVSNSKFLGRIYLEFNRQYNAIIGGRGTGKSTVLEYLRWGLCDQYPDL
                     SQEDEELPNYQERRKKLIERTLLPYDGTVQVSFIKNGIVHVVRRKAKSGEILLKIGNE
                     DFEVCTEENIRNLLPIQAYSQKQLSSVGVSGTELKRLVYSPIRQNLSEYDLHFKNLNT
                     DIRACFEKRNQINLLQKEIHRRELELKSVTEQVENLRKNLKGISDEDRATIDTHEAYS
                     ETEQLVEEWVSELTAASNSLDVLLTEIEAFPTPIPPRLIIPDASKDTIKTINDSISQL
                     FKDVKKQLNDIKKNIAEYSAAENEFSHGRTDWMQLLLIHKQHYESAKERSSSQQATLT
                     QISQLEKRSNEIRKSLGEKKQLLNKLGDQDQKFSRLKEQWVSSHNGRADTIQAQCEQL
                     SILSDNNLRATLGRGKGIDSLGSTLRSILAGSNIRKEKTDSICSFVSNATNPINEWQE
                     LLNEFESLANFDFENDSPETFPSTPKLVSIGLSQNDLRKIAEKMNINNFINMYLTQLE
                     DIPVFEYKTKEGEYIEFTDASAGQQATSLIHVLLNQEGPTLIIDQPEDDLDNQMVSEI
                     AELICKSKKNRQLIFTSHNANIVVNGDAELVACCDYRVSGDQSNGEIKHLGAIDISII
                     RDVITKIMEGGEKAFKLRKDKYGF"
     gene            complement(7322..9442)
                     /locus_tag="PKI50_00600"
     CDS             complement(7322..9442)
                     /locus_tag="PKI50_00600"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNT02248.1"
                     /translation="MDVFELELAGRKLSIETGRIAQLADAAVLVRYGDTVVLVTACAS
                     AEPKEGIDFFPLSVDYEERLYAVGKIPGGFIKREGRPTEKAILSARLIDRPIRPLFPH
                     GYRNDVQVVATVLSVDQDNLPEIAAMIGSSAALSISTIPFQGPTGSVLVGRVDGELVL
                     NPNNEEREKSDLHLVVSGTKDAVMMVEAGAKEVPEDVMLEAIMFAHDNIKKIVAFIEE
                     IVAKVGKEKKEYTVFKPEEEIETAVREYATQKTLDAIRTPDKLERQEKIDAVKEETFE
                     HFAEIFPEKEKDIDEVLYDILKEQVRKLITEEGIRPDDRKLDEIRPISSEVGVLPRVH
                     GSGLFTRGQTQVLTIATLGALGDVQILDGLTEEESKRYMHHYNFPPYSVGETRFMRGP
                     GRREIGHGALAERALEPMIPSEEEFPYTIRLVSEVVSSNGSTSQGSVCGSTLALMDAG
                     VPIKAPVAGVAMGLMKEKGNVAILTDIQGMEDFLGDMDFKVAGTSKGITAIQMDIKIA
                     GIDRPILERALEQARVGRLFILDKMLSVISEPRKELSPYAPKIITTRVDPDKIRDIIG
                     PGGKTINKIIAETGVKIDIEDDGRVFIAAVNQAAGDKALKIIESLVKEVKEGEIYLGR
                     VTRLMTFGAFVEILPGKEGLVHISKLDTKRVEKVEDVVAVGDEILVKVTEIDKQGRIN
                     LSRRHAMADVAKEEEKAEEEKEKA"
     gene            complement(9598..9867)
                     /gene="rpsO"
                     /locus_tag="PKI50_00605"
     CDS             complement(9598..9867)
                     /gene="rpsO"
                     /locus_tag="PKI50_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033016867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNT02249.1"
                     /translation="MALTKESKGELIQKYQIKEGDTGSPEVQIAILTERINNLNEHLQ
                     INKKDHHSRRGLLKMVGQRRGLLNYLKAKDIERYRAIIEKLGLRK"
     gene            complement(10018..10935)
                     /locus_tag="PKI50_00610"
     CDS             complement(10018..10935)
                     /locus_tag="PKI50_00610"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNT02250.1"
                     /translation="MKIAEQYDEAMKSAPWCIALGSFDGVHIGHRKLIEILKEESRLH
                     GTGSMVYTFHTHPRKILIPNKHIYLITDNKKRSEVIEQMGVDMLYLEDFNKIREMRAE
                     DFVKDILVSRFSVKSVVIGYNYKFGIKGEGNVDDLKQYGEKYGFRVIIVDAFEVNGHI
                     VSSSLIRHVIRSGNVDKVSQYLGRDFSVHGEVVYGKQNGELMGFKTANIDVKKDITLP
                     KKGVYYTSTKVCGRLFKSITNIGFNPTFNGESLSVETHLLDFCDDIYGEEIEIFFHKH
                     KRDEMKFDSIEGLVEQINRDIQDRYTFES"
     gene            complement(11073..11984)
                     /gene="truB"
                     /locus_tag="PKI50_00615"
     CDS             complement(11073..11984)
                     /gene="truB"
                     /locus_tag="PKI50_00615"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNT02251.1"
                     /translation="MNGIINVLKPPGMTSHDVVSYLRKVLNIKKVGHTGTLDPEAAGV
                     LPVCVGKATKAVQYLTDKQKSYRANIKFGTVTDTFDSYGQVIKEYESVVIDKKALEDT
                     LKSFTGAISQKPPVYSAIKIKGKKLYQYALEGKEIRIAERPVEIHELKLVEMISEDEA
                     IIDILCSKGTYIRSLCHDIGEALGCGAHMSQLIRLGSSPFSIEDSYTLEEIKAAAEEN
                     RINDILINVEILFKHYKSITVKASALKSITNGNPLYEQGVLNGFEGLNENEDIRIYGE
                     AGFIGTGTVQNDESGQRLFIKIKNIFI"
     gene            complement(11994..12947)
                     /locus_tag="PKI50_00620"
     CDS             complement(11994..12947)
                     /locus_tag="PKI50_00620"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNT02252.1"
                     /translation="MIFQNIISKIKESSKIAILSHIMPDGDSIGSSLALYNALKKCGK
                     DVRFILDDEIPKIYAFLEGAEQVRKPGQFENFDAVIALDCGDAERLGKSSLYLNDNYV
                     INIDHHISNKEFGSLNLVDTGSAASGEIIYELVKSLDIGMDREICECLYTAIVTDTGQ
                     FQYSNTTSVTHQIAGDLIDNGVSVPLMFERIYQNNSKEKVMLMKTALSTLEFFHNDAA
                     SCISLSLEQMKEANALEEDTDGIINLARDIGCVEVAMFLKEIEPGIIKVGLRSKKKAD
                     VAAVALRFGGGGHVHAAGCTVHGTIAEVKEKILEAVAEELR"
     gene            complement(12944..13309)
                     /gene="rbfA"
                     /locus_tag="PKI50_00625"
     CDS             complement(12944..13309)
                     /gene="rbfA"
                     /locus_tag="PKI50_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNT02253.1"
                     /translation="MANHRIDRISEEVKRVVSDIIRNDLNDPRISTMCSVVSAEVTAD
                     LRYAKVFVSILGSDEEKKATMKGLTNAAGFIRRELGRKIELRYLPEVIFELDRSIEHG
                     AYINKLLNEVKKKDGGENL"
     gene            complement(13342..15915)
                     /gene="infB"
                     /locus_tag="PKI50_00630"
     CDS             complement(13342..15915)
                     /gene="infB"
                     /locus_tag="PKI50_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNT02254.1"
                     /translation="MNKIRIYELAKELGVQSKELIAAAKELNINVNNHMSSLEDSEAS
                     AIKKHLKKDDNVEAPKPIQAQQPKKRTLPQKPQQPQQTQQKPQAAQQPQQEQKAQRAP
                     QTAQQPQQAQKAQRAPQTVQQPQQVQKPQQKLQQPQQPPQAQKPQQKPQTVQQPQQRP
                     QSAQQPVRPEQKKDIRPAGKPHQHPSGDKRNPSSDIRPVSAPKNVSAHDEIEEDAITL
                     STKKDFTKVERIGKKLPGKSPQGKPPFRQQQQGKPYNKNFKQRGFEAPKKEEVHVSKK
                     PVKIGDMISLKELSERLGKPVTEIIKKLLLLGIIANINQMLDFDTAQLICEEFGFTVD
                     KVIDKESEEVLLKTEEDRPEDLRPRPPVVTIMGHVDHGKTSLLDAIRQTNVIATEAGG
                     ITQHIGAYTVTIGDRKIAFLDTPGHEAFTAMRARGAKVTDIAVLVVAADDGVMPQTVE
                     AINHAKAADVALIIAINKIDKPGANPDRVKQELTEYGLVSEEWGGDTIMVPVSAKKRE
                     GISELLEMILLVAEMQELKANPNKKGKGTVIEAELDKGKGPVATVLIQDGSLSLGDYF
                     IVGSTHGKVRGMIDDKGKRIKKAGPSTPVEIQGLDEVPDAGDIFIVVDDEKTAKTISE
                     KRKEKQRLSQIQSKQTVSLEELFNQIQEGKVKELNIILKADVQGSVEAVRQSLARLSN
                     EEVKVNTIHGGVGAITESDVMLASASNAIIIGFNVRPQPMAIALAEKEKVDIRLYRVI
                     YNAIEDVEAAMKGMLEPEYKEVLLGHAEVRATFKASAVGTIAGCYVTDGKINRNNDIR
                     VIRDGIVIHEGKLASLKRFKDDAKEVNAGYECGLNIEKFNDIKEGDIIESYTTEAVPR
                     K"
     gene            complement(15908..16249)
                     /locus_tag="PKI50_00635"
     CDS             complement(15908..16249)
                     /locus_tag="PKI50_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNT02255.1"
                     /translation="MNKAYSMISLANKAGKLVTGEDAVRNSIRNGRVKLVIISEDASD
                     NTKKRMSNSAEFYSIPFIVWGLKDEFGISLGKSARSVLGITDESFSNGIKAKLIDEVR
                     KKEKPGGGFIE"
     gene            complement(16242..16511)
                     /locus_tag="PKI50_00640"
     CDS             complement(16242..16511)
                     /locus_tag="PKI50_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNT02256.1"
                     /translation="MQRKRKVPLRKCLGCNEMKPKRELIRIVRSPEGKVDIDKVGKAP
                     GRGCYICPDLTCLEAAIKAKRVENALEAPVDEDVFNRLRTQLSHE"
     gene            complement(16526..17566)
                     /gene="nusA"
                     /locus_tag="PKI50_00645"
     CDS             complement(16526..17566)
                     /gene="nusA"
                     /locus_tag="PKI50_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNT02257.1"
                     /translation="MNGEFLQALEQIAKEKGISKELLIDAIDVALVSAFKKNFGTSQN
                     VKVQINRETGEVDVYALKTVVEEVDNEFMQISLQDAKEIDKNYEAGNVVETKVTPKNF
                     GRIAAQTAKQVVVQRIREAERGIIFDEYISRENEIVTGLVQRKEKNSVFIDLGKTEAI
                     LGPGEQTPNEVYNIGDRIKTYIVEVKKTTKGPQIILSRTHPGLVKRLFELEVPEIQSG
                     IVEIKSIAREAGSRTKIAVYSHDENIDPVGACVGQRGHRVQVIVDELKGEKIDIIQWS
                     NDPEIFVGSALSPAKVIEVFADKETNAARVVVGDSQLSLAIGKEGQNARLAAKLTGWK
                     IDIKSQSQAYGI"
     gene            complement(17591..18052)
                     /gene="rimP"
                     /locus_tag="PKI50_00650"
     CDS             complement(17591..18052)
                     /gene="rimP"
                     /locus_tag="PKI50_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNT02258.1"
                     /translation="MAKRKVEDIVCELAKPIIDRNNFELVEIEYKKEGPDWYLRVYID
                     KEGGITIDDCQSVSEELSDLLDIADPIEQSYIFEVSSPGLDRPLKTDRDYRKNIGKPI
                     EIKLFSPLEGKKIIEGILKGHTESSVEIEVEGKALNVEKASIALIRPLIKF"
     gene            complement(18243..22517)
                     /locus_tag="PKI50_00655"
     CDS             complement(18243..22517)
                     /locus_tag="PKI50_00655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HNT02259.1"
                     /translation="MQNDMKPDSNLFLQYKDKFELKKIRVLKKAREWEMIISLKGFIP
                     IPELGEIESAIAEYFKLNKVRIIIDESSVSLSADELVKSYWEDLKKAVIASNPSFTGF
                     IMDSNVQLENNVIRITVKSEEAAKYLTAKKCNDIIERYINDYFNISLHVSICADNEEK
                     YCEDEYLKCYFEEENKIITEALKPIKSDNSGKAKENKDELIMGKPFKDTPVSIASITE
                     DSGRVTIQGCIFNTEIKTLNNGRTIITFNITDNTNSITAKIFAKEENDFAKLTSNIID
                     GRYIALKGDIQYDKFIKELVVFPISITAAEKSIRTDNAEVKRVELHAHTGMSAMDGIC
                     SASDLIKKAKSWGHKAIAITDHGVVQAYPEAYEASKIHGVKVIYGVEGYFVNDGVPIV
                     YNAVNRSFEEEFTVFDIETTGLYPIKDKITEIGAVKIKQGRITDRFSELINPEIPIPE
                     NITKLTGITDSMVKDKPTIREILPKFLEFAGDSPLVAHNANFDSGFIRAKAAELGLIF
                     NNTIIDTLQMSRVLLGGLKRHKLDIVCEYLGIELENHHRAADDARATAEIMLRFIEMM
                     KSKGINDAADINKLSLSGRSHKTGESYHIILLVKNMTGLRNLYKLISMSHLDFFYKKP
                     RLPKSLLMQYREGLLIGSACESGEVFRNILNNLDENTIEENARFYDYLEIQPNGNNEF
                     LVRSGIAANKGELEAINRRIVELGKKLNKMVVATGDVHFLEPEDEIYRRIIMAGQGYS
                     DADEQAPLHFKTTDEMMEEFEYLGRETAYSVVVRNPNRILEQIEEIIPIPENTYPPKI
                     EGAEEQIREMTMKKAHELYGEILPEVVEKRLDKELNSIINNGYAVMYLIAQKLVAKSM
                     ADGYLVGSRGSVGSSFVATMCDITEVNPLPPHYLCPKCKYSLFFLDGSIESGADLNDD
                     NCPQCGTVLRKEGHDIPFEMFLGFEGDKEPDIDLNFAGEYQPEAHKYTEGLFGKGNVF
                     RAGTIGTIAEKTAYGFIKKYYEEKGIKINNAEANRLIKGCTGIKRTTGQHPGGVMIVP
                     DYTDIYNFTPIQRPADDPSSNIITTHFDYHSISGRLLKLDILGHDVPSIIRMLEDITG
                     FGVNNIPFDDSRTMSLFTSAEALGISLEDIECKVGSLGIPEFGTKFVRQMLMDTQPGT
                     FAELVRISGLSHGTDVWLNNAQELIKSGTATLKEVIATRDDIMLYLIYKGVNPRTSFK
                     IAENVRKGKGLTPEYEEIMREQNIPEWYIESCKKVKYLFPKAHATAYVMMSFRVAYYK
                     VYYPEAFYATYYTVKLEDFDADLFIKGKESIRAKWIEIDKLGNNATAKEKNLMTLIEV
                     VYEMYLRGIKLLPVDIYKSDVKRFMVTKEGILPPLASLQGVGASAAQNIACARQESCF
                     ISMDDLRERARLSKTVIEILKNHGCLEGLPESNQLSLFSAGGNLGV"
     gene            complement(22540..23193)
                     /locus_tag="PKI50_00660"
     CDS             complement(22540..23193)
                     /locus_tag="PKI50_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT02260.1"
                     /translation="MNVTAVIPAYNEEQTIADVVKCVKSIDKIQKVIVVSDGSTDSTA
                     EIARECGADVIELDENVGKGGAIRAGINECGTEIILFLDADLIGLTEKHVLDLIEPVI
                     DNKADMTIGIFKNGRMVTDLAQKVTPYLSGQRAIKKSIIDKIPNIDITRYGVEVALTK
                     YVDKFNVRVQEVDLPDMTHVTKEEKLGIIRGVHARLKMYWDIVKILSSYNIKSGSSK"
     gene            complement(23245..24306)
                     /gene="ispG"
                     /locus_tag="PKI50_00665"
                     /gene_synonym="gcpE"
     CDS             complement(23245..24306)
                     /gene="ispG"
                     /locus_tag="PKI50_00665"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNT02261.1"
                     /translation="MGTYTRKIRIGNRYIGGGEPILIQSMTNTRTQDKAGTIEQIKRL
                     EAAGCDIIRIAVPDMEAAEAVKDIKNKISIPLVADIHFDYRLAIQSIINGADKIRINP
                     GNIGDNERVKKVIDAAKERGIPIRIGINSGSLEKEILEKYGSPTADALVESALKNVRL
                     VESMDFSDIVISIKSSDVLLTIDSYIKASKAANYPLHLGITEAGTLLAGSVKSSVGLG
                     ILLYNGIGDTIRVSLTGDPAEEIYAAKEILKSLKLLKEGIEIISCPTCGRTCVDLIRI
                     TEEVEKRIKGIEAKKPMKVAIMGCAVNGPGEAKEADLGIAGGQGEFLLFLKGKTVRKI
                     PQENVIEELIKEIEALQSV"
     gene            complement(24315..25322)
                     /gene="rseP"
                     /locus_tag="PKI50_00670"
     CDS             complement(24315..25322)
                     /gene="rseP"
                     /locus_tag="PKI50_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNT02262.1"
                     /translation="MTLIAAILVFNLIVFVHEAGHFAAARLGGIKVVEFALGMGPKII
                     GKKFGETVYSLRAFPIGGFCLMLGEDEENNEPGAFNNSPIISKISSVISGPVFNIILT
                     ILIYSLVIAPVAAPIVGQVTKGMPAETSGIKAGEMIVGINDVKINEWKEMKPEIAKHE
                     GEQITVTVEKNGVQREVMLTPVKNPGTDDIVIGITQKVGISGFSVKEGINTTYAVSKM
                     MLSFLGQLVVGKADANEVSGPISILVYINEAAKAGFIKVLYLTALISLNLGIINLLPL
                     PALDGGRLLFLFIELIRRKPLPAEKEGMVHFVGLIALMALSIFLMYRDIIRFNLMDIF
                     R"
     gene            complement(25322..26482)
                     /locus_tag="PKI50_00675"
     CDS             complement(25322..26482)
                     /locus_tag="PKI50_00675"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNT02263.1"
                     /translation="MVKVLSVLGSTGSIGTQTLDIIRENKEEFKIDALAANSNIDLLE
                     AQVREFRPKIAAIGDMSKYKEFKSRVGGMTCVAAGLEGVVEAATLESCDTVVSAIVGI
                     AGLIPTYNAIKTGKNIALANKETLVTAGRIITREVKKNNVNMLPVDSEHSAIFQCLAG
                     SRHNEISKIILTASGGPFRNKTLKELESVTVQEALKHPNWSMGRKITIDSATLMNKGL
                     EVIEARWLFGIMPESIEVCVHPQSIIHSAVEFVDGAVIAQLGLPDMKVPIQYALTYPE
                     RLPMKSAKLSLTSVGELTFYKPDTEKFKCLRLAFRALDMGDSACVVLNGANEEAVKLF
                     LSGKISFLDIGNLIEKTLDKHKIIENMDIEEVSELDRWSRETLAYLYETGCK"
     gene            complement(26479..27285)
                     /locus_tag="PKI50_00680"
     CDS             complement(26479..27285)
                     /locus_tag="PKI50_00680"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNT02264.1"
                     /translation="MLKTRVLSALLGIPLIIFILYSGGLYLYLFTAAVSLTGLFEYYR
                     ALSNINISTNKIFGYTIVILYYIMFLLPIAFNRPGFLIALSVITLLIYEIISKKHSFT
                     EISINIFGIAYIPFLFSHLLFIENLRYGNIILWLPFLTAWFTDTFAYFIGIYMGRRKL
                     CPAISPKKTVEGALGGIAGSVAFSIITGIILNNYGIEIRLIHFIITGFLCGIASETGD
                     LAASYIKRYAGVKDFGNIIPGHGGILDRFDSILFTAPIIYYYFVLIGDLI"
     gene            complement(27297..28019)
                     /locus_tag="PKI50_00685"
     CDS             complement(27297..28019)
                     /locus_tag="PKI50_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNT02265.1"
                     /translation="MLGDIDKGKLPVHIAIIMDGNGRWAQKKGLPRVMGHKAGMEALK
                     KTVKSCSDLGIKILTVYAFSTENWNRPQDEVNYLMDLLVEYMRREVNALHKNKVKIKI
                     LGEVDMLPDQTRTEIEEAIELTKNNEGLQFNIALNYGGRAEILRACRNLIKDLEAGNL
                     DMDSADEKVFSNYLYTSNDPDPDLIIRTSGEQRISNFLLWQGAYSELLFVDQLWPDFD
                     EAVLHSAILEYKNRSRRFGALK"
     gene            complement(28062..28262)
                     /locus_tag="PKI50_00690"
     CDS             complement(28062..28262)
                     /locus_tag="PKI50_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02266.1"
                     /translation="MDNIPLVIGYTLDHALQIIGEKHDIIIDSTSTPFEDKKKERRGN
                     SPIVVRQKAECDKIILTTSCFK"
     gene            complement(28269..28826)
                     /gene="frr"
                     /locus_tag="PKI50_00695"
     CDS             complement(28269..28826)
                     /gene="frr"
                     /locus_tag="PKI50_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNT02267.1"
                     /translation="MIKDIEKNIQEKMEKTLTVFKHELSTLKAGRANPSLLDRITVEY
                     YGAPTPLNQLANIAAPEPRVLTISPWDAKSIPVIEKAILKSDLGINPSNDGKMIRLVI
                     PQLTEERRKELVKVVKKLGEESKVAIRNIRRDANEHLKKLKKDGDITEDDLKKSEESI
                     QKITDKFIKEADKAIEAKEKEILEV"
     gene            complement(28831..29544)
                     /gene="pyrH"
                     /locus_tag="PKI50_00700"
     CDS             complement(28831..29544)
                     /gene="pyrH"
                     /locus_tag="PKI50_00700"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNT02268.1"
                     /translation="MSDVKYKRVILKISGEALAGEKSFGLDHDMLLDVAERVKLLREL
                     NVEVAIVVGGGNFWRGRTSEGMDRTTADHMGMLATVINALALQDALEGINVPTRVQTA
                     IEMRQIAEPYIRRRAVRHLEKGRVVIFASGIGNAYFSTDTTAALRAAEIEADIILMAK
                     KVDGVYDSDPVINKEARKYDKLSYMEVINKGLGVMDSTAASLCRDNKIPILVFDLKNP
                     DNIIKAIMGEKIGTVVKED"
     gene            complement(29655..30302)
                     /gene="tsf"
                     /locus_tag="PKI50_00705"
     CDS             complement(29655..30302)
                     /gene="tsf"
                     /locus_tag="PKI50_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNT02269.1"
                     /translation="MISASAVKELRERTGSGMMDCKKALTESNGDMEKAIEILREKGL
                     AAAAKKAGRIASEGIVDSYIHGGGRIGVLIEVNSETDFVAANEEFRQFVKDMAMQVAA
                     SNPLYVKREDVDPKVIEKEREIYKAQALNEGKPEKIIERMVEGRIEKYYKEICLLEQP
                     FIKDTDKTVQEILTSLIAKIGENITIRRFVRFERGEGLEKKEDNFVDEVMSQVNK"
     gene            complement(30369..31067)
                     /gene="rpsB"
                     /locus_tag="PKI50_00710"
     CDS             complement(30369..31067)
                     /gene="rpsB"
                     /locus_tag="PKI50_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNT02270.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTVKKVEEAYNFIKEVAASGKDILFIGTKKQAQESIESEAIRANMFYVNQRWLGGMLT
                     NFKTIRKRIVRLNELEKMEEDGTFEVLPKKEVINLRAEKEKLQKNLGGIKNMGENPGA
                     IFVVDPRKEKIAIQEAHILGIPIVAIVDTNCDPEEVDYPIPGNDDAIRAVKLLTSKIA
                     DAVIEARQGEQLAE"
     gene            complement(31272..31673)
                     /locus_tag="PKI50_00715"
     CDS             complement(31272..31673)
                     /locus_tag="PKI50_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02271.1"
                     /translation="MGNYNLKNIMLGLGIGLVLASMMNISMGNKELTLEEIKKEAQKH
                     GLVVFSKEEIIDSRNQENTAPTEKVTVEIKSGMSSESIAALLRQKGIINDSGAFIDKL
                     KETNNETKLKEGTFEIPKGSGYDEIISILTK"
     gene            complement(31673..32182)
                     /locus_tag="PKI50_00720"
     CDS             complement(31673..32182)
                     /locus_tag="PKI50_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT02272.1"
                     /translation="MINYVLMAIGVFVILAASIMIFLDKVKGEDIYFSMDVKEQEIKK
                     VIEDADEIISELNYTSEMVVQEIEEKLSSFREELKSLNNQVPGTSSADNKPAAPVVPN
                     VSAANKKKKIINDNSDLDKPVDEKLSQKQLKVFEYAAQGMSVTDIAKQMNIGQGEVML
                     ILSLKNEVE"
     gene            complement(32196..32516)
                     /locus_tag="PKI50_00725"
     CDS             complement(32196..32516)
                     /locus_tag="PKI50_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02273.1"
                     /translation="MSLRPVDLQTIIPKLPEVQKAKNAENELEKNNLSINMHKEQQQQ
                     DKNTQQIIETKKAQEARINRDGKQNRGQGDQKGEHKDSRPEDNEGQENKKNKDPMSTI
                     DIRI"
     gene            complement(32539..34347)
                     /locus_tag="PKI50_00730"
     CDS             complement(32539..34347)
                     /locus_tag="PKI50_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNT02274.1"
                     /translation="MSNEYIILEGKDYEKLIAKGMEHFNARKEELNIEILESKKNLFS
                     SYYKVKIAKGENKTLEIIESNIDSILNENLKANTKSIDFDFREDGVYIKADKDVTIVD
                     IVTKIDLRRIQSVDLTKIKQMLEIGSLDNWIKIAEPQMEKKIDSECLVKTAKDKMKAF
                     IVISKPIGGKDITLEVINEALKQEGVKFNIKEDVIEKAVSQKVYDKEILVAEGIEPQP
                     GKDAELVYHFDTSAEKSIGIDKDGKIDYHELSLIKNVKAGEKLVTLMPHTEGIPGKNV
                     SGEDVPGKDGKKLALPRGKNVVVSEDELELVSAIDGEVKIIDGKVNVFSVYEVKNNVD
                     NSTGNIRFNGKVIVMGNVLTGFLIEAEGDVEVYGVVEGAQIKSKGNIILHRGIQGMNR
                     GELYCEGDLVAKFIENSKIDVKGNIQSDAIMHSQVTCGKKLEATGRKGLLVGGTFKVG
                     EEIRAKVVGSPMATITELEVGVNPEMRNKFEELKNELKQVTDNLDKTSKAVDLLTKMS
                     KSMELPEDKKALQAKSLQLKLQLKQKQDQLLNEQNELEVYFEELSKGKVKVYDIIYPG
                     SRIIIGSSMMYIKDSIKFVSFCRMHAEIKMLSYNDI"
     gene            complement(34364..35098)
                     /locus_tag="PKI50_00735"
     CDS             complement(34364..35098)
                     /locus_tag="PKI50_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNT02275.1"
                     /translation="MVNLWEVYSRTKAAEIREELILKYTYIVKYVAGRLYSSYGNNVE
                     FDDLVSYGIFGLIDAIDKYDIARGVKFETYAQLRIRGAIIDHLREIDWLPRSIRQKSK
                     ELEKSYNDLESRLGRPATDEEMAESFGLSVEEFQKKIQNITTYSVVSLDDLLEQKREI
                     ISNEADKHSETPENVIEDKEMKEILADAISALPEKEKKVVSLYYYDELTYKEIGKLLN
                     ISESRVSQLHTKAIIRLKNKLKIAFE"
     gene            complement(35130..35498)
                     /locus_tag="PKI50_00740"
     CDS             complement(35130..35498)
                     /locus_tag="PKI50_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02276.1"
                     /translation="MRISDFIPILIVITALIVTVTLGICNNIDFSELMIRCMAVTIVF
                     GIFGSLITKTVLNLIEYYRVSIHIQDKGGTVSATGSKLDIKVPPLDDEALEAIDYDSD
                     NEFIEVNPADLSKYINSNTD"
     gene            complement(35532..36017)
                     /locus_tag="PKI50_00745"
     CDS             complement(35532..36017)
                     /locus_tag="PKI50_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNT02277.1"
                     /translation="MIDIVKVGMADLNTTFCPGVLTTLGLGSCVGICLYDPSTKIAGM
                     VHIMLPSSAQIKNNSNEAKFADTGIVKLITEMIKIGAKRNKLVSKIAGGAQMFNFNDT
                     SEVMRIGLRNVTATKETLKMLNIPLISEDTGGNYGRTIELYSETGILLIKTIGYGTKQ
                     I"
     gene            complement(36019..36633)
                     /locus_tag="PKI50_00750"
     CDS             complement(36019..36633)
                     /locus_tag="PKI50_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNT02278.1"
                     /translation="MGISIEELNSLQIDVLKEIGNIGAGNAATALSKMISKRIDMDVP
                     KVNILEFKNVAELVGGPETEVVGIYFKVNGDITGSIMVLVDKKSAKMLANLLMGRDSN
                     GDDLDEMDLSALQEVGNILSGSYLSSLSTLTNLNLTMSVPSLAIDMAGAIISVPVILF
                     GQVGDRVMLIETDLFEGTYHVKSNFFLIPDEDSFEILLKSLGVF"
     gene            complement(36648..37091)
                     /locus_tag="PKI50_00755"
     CDS             complement(36648..37091)
                     /locus_tag="PKI50_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT02279.1"
                     /translation="METATFARQFVIFKLENEEYGIDILRVKEIKEMMSITRVPNTAH
                     YVRGVINLRGEVIPVIDLRKRFNLPEGKDSNNTRIIIVSVDDIIVGFTADYSSEVVEI
                     SSDAIEEAPDGVGSVDQGYISGIGKVGERLIILLDIVKIITNPAV"
     gene            complement(37105..39120)
                     /locus_tag="PKI50_00760"
     CDS             complement(37105..39120)
                     /locus_tag="PKI50_00760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNT02280.1"
                     /translation="MDVNQYLEIFIEESLEHLQELNQNLLSLETNPGDMKTLNEIFRV
                     AHTLKGMAGTMGFTGIANLTHQMENVLDAIRTGNIEVNSSMIDILFECLDYLSNSINT
                     ISATGKESSSNVNSIIAGLNGMLAKKPEAEASDTADKKEPQESKMKFNQYDDNIIRKA
                     VQQNHGVYKITVVLAKDCMLKSARAFLVFQTVERYAEIFKSEPKVEDIEDEKFDNEFT
                     IAIITKETEALIRKELESISEVTEVIFERIEVPEELDVKDIDEITNEEKETLKYSIED
                     KENGDYIQGKKARMGKTVRVDIDRLDKLMNLVSELIIIKTRLEGVGTDSKNQDMIEAV
                     EYLERITTNLHDAVMKVRMVPIERVFNRFPRMVRDLAKSLSKDIILNMSGEDTELDRT
                     VIDEIGDPLIHLIRNSIDHGIEEKEERIKNNKPANGTVNLRAYQDGNSVVIEVEDDGA
                     GIDIEKIKRKAVERGILTSSEADDLDEKSAIELLFNPGFSTADKVSGLSGRGVGLDVV
                     KTKIESLNGLVEVENYRGKGSKFIIRLPLTLAIIQALLVNIGEEKFAIPLNVIRDIID
                     IEASTIRNVHGQDVVLNRGNVLPIVRIAKMLGVINASSDDSDQLTAVVVKKGEKNAAF
                     IVDSLMGQQEIVIKTLGKFLSGIRNIAGATILGDGQVALIIDSNSLI"
     gene            complement(39125..40207)
                     /locus_tag="PKI50_00765"
     CDS             complement(39125..40207)
                     /locus_tag="PKI50_00765"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNT02281.1"
                     /translation="MLSGKEKIRVLIVDDSAFMRVLISDMLKNDADIEVAGTARNGKE
                     GLEKAILLKPDIITLDIEMPVMNGLEALVELMKLKPVPKVIILSSLSYEGGEAAIKAF
                     ELGALDYVVKPVRSLITIDAEQMREELINKIKSICWSKTGYFPQIKPEYKPPSVYKKV
                     KHKSRSDEYLKYIVAIGTSTGGPKALQEVVTKLPEDIPAAVLIVQHMPPGFTKSLANR
                     LDSLSEINVKEAEDGDVLTSGWAYVAPGNYHMLVKSHEDNKYKILLNSEAPENGLRPS
                     VNVMMKSVAETGHDKIIAVIMTGMGSDGSEGILEIKNAGGKTIAQDERTCVVYGMPKS
                     AVNTGAIDTIAPLHGITGEILKCMGV"
     gene            complement(40209..40883)
                     /locus_tag="PKI50_00770"
     CDS             complement(40209..40883)
                     /locus_tag="PKI50_00770"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNT02282.1"
                     /translation="MKGLTKYFNIGDKVLIEYFDAVGELRQYVSQIVDIHDGQYEEFF
                     DVLIPIYKNMDVNIRQGTVLKLIVFKGEAIYEIRTILFKKLFGSIPLLRLKAASEVIK
                     KQRRDYYRLKIFREIEARLVINPEEKQYGEKFKCTIQDISAGGLMFSSREYFNENDTL
                     EFALDLNGPIMVVQGVIVRRILNESYRLLYTYGVQYINLNMADRNAITKFIFEEQRRL
                     IKKGLI"
     gene            complement(40960..41862)
                     /locus_tag="PKI50_00775"
     CDS             complement(40960..41862)
                     /locus_tag="PKI50_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNT02283.1"
                     /translation="MKDQAERLREIISSIKNYKDEEKRTGILTDKPGSRVIAVTSGKG
                     GVGKTNFTVNLGIKFAQMGLRVVIIDADLGLANVDVVMGKMSKYNLSDVINSNKNILE
                     ILEEGYEGVKFVSGGSGVQELVKLNRTQLVDLLMKLGRLDEEADIILIDTGAGLSENV
                     ISFVHAAREVILVTTPEPTSITDAYALIKTITHKDKTKNIKVVVNRADNAAEAFNILD
                     KLNIVTQKFLGVKLQKLGYILNDTCVTKAVKIQQPFVISFARSEASKNMNDIALTLMD
                     NKELSPSSVSGIKMFINKLTSLFN"
     gene            complement(41846..43054)
                     /gene="flhF"
                     /locus_tag="PKI50_00780"
     CDS             complement(41846..43054)
                     /gene="flhF"
                     /locus_tag="PKI50_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNT02284.1"
                     /translation="MKVKRYVGETAQEAMQKVKSDLGRDAIILNTRKIRKKGLAGLFS
                     KPLIEVVATIDNDISSGARTNNARNDHTIETNEFIKELNENLQVQKNSLKDNTKKHTH
                     LDDKELIQQDFMELKNMLNKVYDAVKVDYENTKLSDVVKKYLKTLEDNEVDKVIINEI
                     KEKLMETLDSEKQQSDAIVRNTVYNILMKYVKEPEPFSFAGGKKVIIVIGPTGVGKTT
                     TLAKLAANMVLTEKKRVGLITSDTYRIAAVEQLKTYSEIIGVPLSIIYTPGEILNAIE
                     SYAEKDLILVDTAGRSHKDKYQLMELKSLIKSSINYEVYLVISATTKFSDCIEIIKNY
                     SFLDDYKLLFTKLDETSAFGVILNVAYITKKPISYITTGQSVPDDIEIADNGKIINCL
                     LGDKLYERSS"
     gene            complement(43054..45081)
                     /gene="flhA"
                     /locus_tag="PKI50_00785"
     CDS             complement(43054..45081)
                     /gene="flhA"
                     /locus_tag="PKI50_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNT02285.1"
                     /translation="MGRYSDVIAVLIVALIILIVIPVPTILIDILLTLNISLSLVIFL
                     LSMYIKEPLQFSIFPTILLITTLFRLCLNFSTTRLILGKGYAGEVINAFGSFVTGSNP
                     IVGFIMFLILVIVQYIVITKGAERVSEVSARFTLDAMPGKQMAIDADLNSGLINEMEA
                     KERRKAIQQEADFYGAMDGASKFVKGDAIAGIIITVINIVGGFAIGMLQMDLDFIESL
                     STFTNLTIGDGLVTQIPALLVSIAAGMVVTRAASNSDLGTDIIAQLLSEPKVLYIASG
                     ALLILAFTGLPPVPNILLASILAYMGYTLRKTIKAAQKEEEIKAQDVEVEEIRKPENV
                     MSLLQVDPIELEFGYGIIPLADVNQGGDLLDRVVMIRRQCALDLGLIVPVIRLRDNIQ
                     LKPNDYVIKIKGNEVASGELMFDNYLAMNPGTADGELDGVKTTEPAFGLPAVWISENQ
                     KERAEMMGYTVVDPPSVIATHLTEIIRNHAHELLGRQDVQKIIENVRENYPALVEDVI
                     PKQLSIGDIQKVLANLLKEGVSIRDMVTIMETLADYAPMTKDTDMLTEYVRQSMRRNI
                     TKRFIVEMQAKVITLDAVLEQTIMDSVQQTEYGSYLNIEPNTAQQIINNLLKEMQKLT
                     SLGEQPIILASPVVRLYFKRLTEQVAPGLIVLSYNELDPLVEIQSVGVVSI"
     gene            complement(45096..46187)
                     /gene="flhB"
                     /locus_tag="PKI50_00790"
     CDS             complement(45096..46187)
                     /gene="flhB"
                     /locus_tag="PKI50_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNT02286.1"
                     /translation="MKDYAYLKINLQLFGEKTEPATPKRRQELRQKGQIPRSRELVTA
                     VVLLISFFSMRFLSKFIFGDLIFAVRNSLSFPKDIDSRFTTNEIMLIFLQNLLIFAKI
                     LAPILLIVALCVIIMNYVQIGSVLTAKPLMPNLNKINPVEGFKRLFSKNAYVELLKSI
                     FKIGIIGFIIYDYLVDNYRVIPELLNMNVESTMVFIGNTIISVGIRAAAVLLILSVFD
                     YGFQKWNFEKEIRMSKQEIKDEYKLIEGNPQIKSKIKEKQRQLSLRRMMAEVPSADVI
                     ITNPTHFAIAVKYEEAVSEAPVVIAKGKDLIAEKIKDTAKKNNVPIVENKPLAQALYK
                     SVEIGQQIPSELYKAVAEVLAYVYSLKDK"
     gene            complement(46184..46975)
                     /gene="fliR"
                     /locus_tag="PKI50_00795"
     CDS             complement(46184..46975)
                     /gene="fliR"
                     /locus_tag="PKI50_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNT02287.1"
                     /translation="MIMDSISVVLVDRFLVLLLVFIRMTSLFVITPVFGRREMPAYLK
                     IGLAFFCSYIMVPLLGDVRIDYSNIFSFSAIIAKEFLVGIIIGFVSFMVFSALYLAGQ
                     IIDMQVGFGMVNVLDPTTNTQIPMIGNFIYIMTTILFLAIDGHHVLLAALYKSYSVLP
                     IDGFAFTEAMTNNIAAIFTEVFLIGFKISIPVMAATLLAEVALGILSRTVPQMNVFIV
                     GLPLRIGIGLITLYIMIPVFIQIITVTFDRMYGYVYLIIRSMAKG"
     gene            complement(46972..47241)
                     /gene="fliQ"
                     /locus_tag="PKI50_00800"
     CDS             complement(46972..47241)
                     /gene="fliQ"
                     /locus_tag="PKI50_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNT02288.1"
                     /translation="MDQGIVIDVAQEAIKSVLLVSAPILGLGLLVGLIVSIVQATTQI
                     QEPTLSFIPKIIAVSLTMLVFGPWMMNIMYEFTVRLFENIPVYIK"
     gene            complement(47256..48020)
                     /gene="fliP"
                     /locus_tag="PKI50_00805"
     CDS             complement(47256..48020)
                     /gene="fliP"
                     /locus_tag="PKI50_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934844.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNT02289.1"
                     /translation="MYKKNGIIKIILILVLMILMGIIDVWCEPLPIPKIGLDIETAEN
                     PQDVALSMQILFILTIISLAPSILIMLTSFTRIIIVLSFTRNALGTQQMPPNQVLIGL
                     ALFLTFFIMSPVITEINENAFTPFTNGEITQEAAIEKAAEPIRNFMFKYTREKDLALF
                     LNLSQVKQPVAKIDIPTSALIPAFIISELKTAFEIGFFLYIPFLMIDMIVSSTLMSMG
                     MMMLPPIMISMPFKILLFVMVDGWNLIVRSLILGFN"
     gene            complement(48013..48414)
                     /gene="fliO"
                     /locus_tag="PKI50_00810"
     CDS             complement(48013..48414)
                     /gene="fliO"
                     /locus_tag="PKI50_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNT02290.1"
                     /translation="MNTGSTIYGILYFLFMSAVILAAAYYATRFLSKRSLKNAGNKNL
                     KIVEAAPLGIDKSILLVKVGEQYLLLGSTQKSITLLTEVDPEKLAVGDDESIEVYMSS
                     FQDENKKNGMNSIKNNLNKLKSIVRGNKTDV"
     gene            complement(48430..48792)
                     /locus_tag="PKI50_00815"
     CDS             complement(48430..48792)
                     /locus_tag="PKI50_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT02291.1"
                     /translation="MANGILIVDDAAFMRMMIKDVLSKNGFEISGEAENGAKAIEKYK
                     EINPDLVIMDITMPEVDGIQAVKEIKKINSAAKVIMCSAMGQQAMVIEAIQAGAKDFI
                     VKPFQAERIIEAVKKVLG"
     gene            complement(48828..50006)
                     /gene="fliY"
                     /locus_tag="PKI50_00820"
     CDS             complement(48828..50006)
                     /gene="fliY"
                     /locus_tag="PKI50_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HNT02292.1"
                     /translation="MSEMLSQEEIDALLKGDSATAPQDNSSSQAGHDSVLTTEEIDAL
                     GEIGNISMGTSATTLFTLLNNKVSITTPKVSVVTWEELSIQYPIPYVAVQVQYKEGLE
                     GSNLLILKEDDVKIITDLMMGGSGEISPDSVGDLQLSAIGEAMNQMIGSSSTSLSSMF
                     NKRIDIYPPRAFILYFQNLSELEQFNSADKVVKIAFKMEVGDIIDSEIMQLIPIDFAK
                     ELVRSLLDNISTESEPIFVGKAEEPQVKQPAVSDPGTQPQKPESNTILSKGYEEREEY
                     EPAARREERKVVNVQPVQYQTFDVEKLNAEKESIELIMDVPLEITVELGRTKKLIRDI
                     LEFSPGTILELDKLAGEPVDIMVNGKYIAKGEVVVIDESFGVRITDIVSASKRLSRIQ
                     "
     gene            complement(49999..50994)
                     /gene="fliM"
                     /locus_tag="PKI50_00825"
     CDS             complement(49999..50994)
                     /gene="fliM"
                     /locus_tag="PKI50_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNT02293.1"
                     /translation="MSEVLSQNEIDALLSALNSGEIDVQEIKEEKEERKVRDYNFKIP
                     NKFAKDHTRTLQIMHENYSRLLQTYLSGYLRALVQIEVISVDQLTYNEFTNSMPNPSV
                     LGIVEFAPLTGSIIVEMTPTISFAMIERILGGAGHAFERVRGFTEIELSLLEKIIGQI
                     INFFKDPWKNVIELRPRLKKIETNPQFAQILSPNETVALITLNTKIGSIEGMIHICIP
                     HLVIEPVIPKLSTKFWFSGITKEITGSDLKIIEKKIQSTLLPIRVVLGDSEITVRDFL
                     ELGAGDVIQLNTSVNDDLKIYVGNLKKFSGKPGLKKNKVSVKITRIEKKGDDYDE"
     gene            complement(51011..51436)
                     /locus_tag="PKI50_00830"
     CDS             complement(51011..51436)
                     /locus_tag="PKI50_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNT02294.1"
                     /translation="MKRNIILIAAIVLLLLVIVSGIIIYMFFNKPKVAEEEPIKIDKK
                     LMMLSLGDSFVNNVKDSKKICKLTFKLEVDKEVAELVTYRESEIRDRINALIRSKTEA
                     DLEGKEGQEKLQKEIVEIIKKIINSKKIKNVYFDEFIVQ"
     gene            complement(51456..52178)
                     /locus_tag="PKI50_00835"
     CDS             complement(51456..52178)
                     /locus_tag="PKI50_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT02295.1"
                     /translation="MARKRVQDEPSPGAPAWMSTYGDMVTLLLCFFVLLFSFATLDVQ
                     KFEAIKQSMSGSLGVLDSGMTLSMEPLANAFPADSPTEEAEEFKKLYEQMSEYIKENN
                     LEASITLRLDERGLLVRFMDNVLFDSGKADLTPNASDIINKVAEIIRQNDKIIRIEGH
                     TDNVPINTFRFPSNWELSTTRAVNVVKYLIEENGIYAYRLSASGYSDQHPVDDNNTAE
                     GRQKNRRVDMVILRDDIKDLNP"
     gene            complement(52181..52972)
                     /locus_tag="PKI50_00840"
     CDS             complement(52181..52972)
                     /locus_tag="PKI50_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT02296.1"
                     /translation="MDLASVFGLVGGIAVILITILISGDLIGYVDIPSIICTFGGTIA
                     MTFMAFPIKKLKEGIAALKYVFVYAETDAEYIIKSIIDLANTARKEGLLALEEAAQQL
                     KDDFLQKGILLIVDGTDPELVRNILETELSFIEERHKSNQGVYEFMAAIGPGFGMLGT
                     LIGLINMLANLSDPSTVGPNMAVAIVTTLYGSIIANVFCNPVISKLKIRSSQESLMKE
                     VMLEGMLSIQAGENPRIIEEKLKAFMSPKLRNMLDSAKEPKEGEE"
     gene            complement(52989..53201)
                     /locus_tag="PKI50_00845"
     CDS             complement(52989..53201)
                     /locus_tag="PKI50_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNT02297.1"
                     /translation="MIKVTRLNGIVYYLNCEHIETLENTPDTVITLRDGKKLLVSESP
                     EEIIEKIIEYKKRIFDLSSLIIKDNF"
     gene            complement(53266..54651)
                     /locus_tag="PKI50_00850"
     CDS             complement(53266..54651)
                     /locus_tag="PKI50_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNT02298.1"
                     /translation="MMRSMFSGVSGLRAHQLKMDTIGNNIANVNTVGYKSQRATFQEV
                     FNQTLRGAGSPQAGKGGTNPQQVGLGISLSSIDTFHVRGAVQRTDNLTDLAINGDGFF
                     ILSNSEDGLSRSYTRAGNFSLDDDGNLVSANGFKVLGYMVDPDTNVLRSQLEGVKISK
                     SEMVAAKETTYATFEGNLDNQIAVAPGADADYVFTAGGDENTPDTLTIPADTKYWETT
                     STVYDTLGGKHTLKFTFIRGMEADGAVGGGPSQWQVFIKDQDLGKYYNESGGFAASDT
                     LETITHGLTFKPDGTLDTTSTNGIVELTVPGKNGSGDIEDLKIDFTKLTQFANESNAA
                     MTGSDGYPQGSLDTFSIGPTGEINGIFTNGMSKIIGQIALAAFKNPAGLEKTAENMFQ
                     VTPNSGDPIIGLPGFSGLGSLNSGTLEMSNVDISNEFAEMISTQRGFQANSRIITTSD
                     EMLQELVNLKR"
     gene            complement(54730..55119)
                     /locus_tag="PKI50_00855"
     CDS             complement(54730..55119)
                     /locus_tag="PKI50_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HNT02299.1"
                     /translation="MSVIYNNKPIIPIGKPIQPTKPSDGRQSDKSISFDAVLQQELSR
                     QSEVKFSKHAAERLQMRNIHLTKDDLNKLNEAVNKAAEKGVKETLIIMGNSAFIANVK
                     NKMIITAATEDNLKNNVFTNIDGAVII"
     gene            complement(55141..55797)
                     /locus_tag="PKI50_00860"
     CDS             complement(55141..55797)
                     /locus_tag="PKI50_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNT02300.1"
                     /translation="MPVSGINGQYTAYEPNKPKEYNSNLDKDAFLKILVTQLRYQNPL
                     EPTKDNEFIGQMAQFSSLEQSQNSNKAIRMNSANNMVNKLIKANYRAEDSTETKELIG
                     LVEKVMLKDNEIYLTMDVLGTKYDVKFDDVREVTELENSVEQIYLMNQTMRSTTAFNL
                     IGKNVKGTYEETEKVNGVERIKTVEIEGTVEKVRKEGTSIYLTVNGKEIYLEDITEVN
                     "
     gene            complement(55809..57365)
                     /locus_tag="PKI50_00865"
     CDS             complement(55809..57365)
                     /locus_tag="PKI50_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNT02301.1"
                     /translation="MTFSIGPMPAFNVSKPDNSSNAAFFQGRPENEFSSFREIFRNTL
                     KSGDRYDTKTAAEEDTKMELAGRAEIRHKKSSNRDPEPVNVGDENPAAEKEVRFKDVI
                     ANDVIAEGANVEKAVADDEAVKEAIVKELLMKLEKLNKMKDGENTDAEKQALLEGIKE
                     LLQELAEIRTEISGEEAAGICEMVSELENVPSKLRDVIIAIFEKPAGTKAAVSAEVQN
                     DIIPTEELNSEKNPVKVPTPEKADAKDNLGDESSKMPAPDESQKNDVKTVSSSKPSVP
                     EQKGDKAETDAKEENIKAAMDDKVEKVSVEENPEKVQETGKNNNRKDAAKETHDTINT
                     THNKLDMEGTAVRQEQNIIFSKAETVQNQAEMPKAQPVSKVEIINQIVKKAEVILTGT
                     QSEMKMQLEPENLGKLTLRIAVERGVITAKFTAESYEVKQVIESNFNELRDMLQDKGL
                     DIQNLSVSVGHESKEFNFSKNYETWRDKLKSGAQNMRHDTYGGYQDEIGTTAAINPYS
                     IHTGRFDQRA"
     gene            complement(57380..58021)
                     /locus_tag="PKI50_00870"
     CDS             complement(57380..58021)
                     /locus_tag="PKI50_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02302.1"
                     /translation="MNQPSNTNEKKPAAEKSKKADSKRGKTREKKANTLLLLIIVIFL
                     SAIVAFGAIFMFNFAGLRAETSVWISNLPVVGKLLQPVLQKKNPEQIEREVIEREKSN
                     IAIQQKELNNLEKELLSREKEIEKKEKELADKELEISKLHEQLSEKLRSIQEQVQYLE
                     KVDNQKAMQIILNMNDKASAVQILRNMKKEKAASILAQMDPLQAAQILEDLAD"
     gene            complement(58034..58477)
                     /gene="fliJ"
                     /locus_tag="PKI50_00875"
     CDS             complement(58034..58477)
                     /gene="fliJ"
                     /locus_tag="PKI50_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNT02303.1"
                     /translation="MAVYKFRLQKLLDYKTKVEEEKKNKLGSAVKRLEKEKHKMAELK
                     KEFSDMQSLFQEKTSQGMAVNELKILANYIDYYKRGIKAQKVRIKMAEDYLSACREEL
                     IAATREKRMIEKLKEMDYSKYLYEEQKKEEKLVDDLVSFKESNKS"
     gene            complement(58487..59800)
                     /gene="fliI"
                     /locus_tag="PKI50_00880"
     CDS             complement(58487..59800)
                     /gene="fliI"
                     /locus_tag="PKI50_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNT02304.1"
                     /translation="MMGIDMEKYNKALLSNELIKYTGRVSQVIGLTIESHGPAVNLGE
                     ICNIYPLKGNTPIKAEVVGFKESIVCLMPLGSMKGVGPGSKIEATGESLSVGVSNDML
                     GRVIDGLGRVIDGKGTINNCTMYPVENSPPNPLSRKRITEILPVGVRAIDGPLTLGKG
                     QRIGIFSGSGVGKSTLMGMVARNTKADINVIGLIGERGREVREFIENDLKEDGLSRSV
                     VVVATSDQPALIRLKAAQLATSVAEYFRDQGKDVMLMMDSLTRFAMAQREVGLAIGEP
                     PVTRGYTPSVFSVMPRLLERSGTSDKGSITGLYTVLVDGDDMNEPITDTVRSILDGHI
                     VLSRSLANKNHYPAIEVLASVSRVMPNIVDDEHKENANEFKNIMSVYKDAEDLINIGA
                     YVKGSSSSIDRSIEKIGAIERFLKQGVDEKTEMEEILNEMRIIVS"
     gene            complement(59807..60562)
                     /locus_tag="PKI50_00885"
     CDS             complement(59807..60562)
                     /locus_tag="PKI50_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNT02305.1"
                     /translation="MSKVYKSRNITIGFPKHLVHVFRKETIKEEECKTINTDDNNTED
                     EEEYANSIVEDAKQMYLKIIEEANSEARRIMEAAEEEARSLLSNSREEGYNEGYESGY
                     LEGKNEAQSIIDEASEIKEFLDRRRESLYKEAEEQIVDLVLSISKKVLGQELSQNKET
                     LLSLVNQALQKCTFKNKLVLKISPSDFEFVKENKYRICKMVEGISDIDIVSDLSLPKG
                     SCIVETPSGEINSSIEVQFNEIKKIFTYLLRNE"
     gene            complement(60555..61571)
                     /gene="fliG"
                     /locus_tag="PKI50_00890"
     CDS             complement(60555..61571)
                     /gene="fliG"
                     /locus_tag="PKI50_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNT02306.1"
                     /translation="MSRGLKGTFNGRQKAAVLLITLGPEKSAAIFKHLKEEDIEQLTL
                     EIANMRTVTPEEKEEVLNEFYQICIAQEYISEGGISYAKDILERALGGQKALDVINRL
                     TASLQVRPFDFVRKADPAQILNFIQGEHPQTIALILAYLKPQQAAAIISSLPQDKQAD
                     VARRVAIMDRTSPEVIKEIERVLERKLSSMVTQDYTIAGGLAAVVDILNSVDRGTEKF
                     IMETLEVQDIDLAEQIRKRMFVFDDIVTLDNRSIQRVTREVENSDWALALKNAGDEVK
                     KAIFSNMSKRLAEMITEDMEYMGPVRLRDVEEAQQKIVNIIRKLEDAGEIVISRGGGD
                     EIIV"
     gene            complement(61583..63178)
                     /gene="fliF"
                     /locus_tag="PKI50_00895"
     CDS             complement(61583..63178)
                     /gene="fliF"
                     /locus_tag="PKI50_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNT02307.1"
                     /translation="MGEFFNNLRNNTVEFWNGLKKGQKVRIIIAGLLSFVLIVSFITY
                     MSRPQMGILYSDLSQGDAGTIIARLKEMNVNTRIEGTTIYVPVKRIDELRAQLAVEGI
                     FGDDILYPEELQATFYETSQDKNQRYLIAKQNKLKKGLSAIEGVEYADVNLYIPEDQT
                     FIIDQNSSEATASLIIKMKPGYPALDRNQVNGIVQYIARSVKGLKPENISIIDENGRS
                     LVPDTGTPNSIISSQMEMQEAVQDKIEKSITKFLEAPFGINNVKVKAAVKLNYDSVTQ
                     NITTYEAPNTEQNEGIVRNMQDIRKEWVDAGQGGVPGTDSNTDINQYAEVDSSKAQYN
                     EASTIVNYEINEIKEQIIKEMGSIESLSVSVLINTLDSEGNPKQIPQELQDRVGALVK
                     YALQGFNYTASTEENEGIEVVFAPFDMELANKIKADQEAERKAVFWERIIMIGTGVAA
                     LLVFGGVIFIMLRKRTGAYGAGSGYAVDSTGRAMSEAALSGEPIAEIDIEDKNEVKKK
                     IEKFVGLKPETVAQLLKTWVNEE"
     gene            complement(63208..63504)
                     /gene="fliE"
                     /locus_tag="PKI50_00900"
     CDS             complement(63208..63504)
                     /gene="fliE"
                     /locus_tag="PKI50_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNT02308.1"
                     /translation="MKINFPVGQFGIDSNLNNNAASRKEALSFTDFLNNAIKDADKLQ
                     TESEQLNQAFAMGLNDNLHQIVIASEKADIALQFTVQIRNKVLEAYQEIMRMPV"
     gene            complement(63541..63984)
                     /gene="flgC"
                     /locus_tag="PKI50_00905"
     CDS             complement(63541..63984)
                     /gene="flgC"
                     /locus_tag="PKI50_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNT02309.1"
                     /translation="MAFLNSINISASGLTAEKLRMDVISRNIANVNTTRTAEGTPYRR
                     QVVVFREGEGSTPFSEYLSDASRRLIGAGVKVTGIKEDKTPFKSVYDPGHPDADENGY
                     VKMPNVDIMTEMVNMISASRAYEANVTAINSTKSMALKALEIGRR"
     gene            complement(63990..64394)
                     /gene="flgB"
                     /locus_tag="PKI50_00910"
     CDS             complement(63990..64394)
                     /gene="flgB"
                     /locus_tag="PKI50_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNT02310.1"
                     /translation="MIDKISNKTLMLEKALDAAWMRNEAISNNIANVNTPGFKKSYVR
                     FEEYMADAQEKFQISGAKKDPRFLPIGKDRVISANPEIVRENSTSMRRDGNNVDIDVE
                     MAELAKNTIKYNALIAQLSKEFAKIKFAINGR"
     gene            complement(64719..65501)
                     /gene="codY"
                     /locus_tag="PKI50_00915"
     CDS             complement(64719..65501)
                     /gene="codY"
                     /locus_tag="PKI50_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HNT02311.1"
                     /translation="MSSTLLEKMRKVNKVLQTSGTQSVSFHDLSKILSEVLNANVYIL
                     SRKGRILGYSLGTSEECEFLKNELLTEKKFPDEYNKSLLSYHETRSNLINKDKLCVID
                     NRERCDMGTNYSTIVPVNGSGERLGTMMLAKYNQEFTDEDLVLAEYGATVVGLEILRS
                     KHDEIEEEARKKAVVQMAIGTLSYSELEAIEHIFKELDGNEGLLIASKIADKVGITRS
                     VIVNALRKFESAGVIESRSLGMKGTHIRILNDKLLEELKKVR"
     gene            complement(65516..66916)
                     /gene="hslU"
                     /locus_tag="PKI50_00920"
     CDS             complement(65516..66916)
                     /gene="hslU"
                     /locus_tag="PKI50_00920"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNT02312.1"
                     /translation="MNELTPKQIVAELDKYIIGQDKAKKSVAVALRDRYRRNLLDEEM
                     RAEVTPKNILMIGPTGVGKTEIARRIAKLVNAPFVKVEATKFTEVGYVGRDVESIIRD
                     LIEFSVRLVKAEKISKVNEKAFDIAAEKVAEILVPSSNKSSMSKNPFEMIFNYGGSKE
                     TESDEETKKKEESLAANRYYVMQNVKNGDLNNENIEIEIEDTSGHSIEMLSGPGMDQM
                     SINLQDMFGGILPKKTKRRRVTVKEALKLLTQQEAQKLIDMDEVIEEAVKKAEERGII
                     FIDEIDKIAGRGNIGGPDVSREGVQRDILPIVEGSTVITKYGPVKTDYMLFIAAGAFH
                     VAKVSDLIPELQGRFPVRVELDSLSKNNFVEILTKPNNALIKQYKALLRTEGININFE
                     EDAVEEIASIAAFMNEQYENIGARRLYTVMERLFEDISYEAPDMKSGELNITKEYVQK
                     ALIHDIKRKDVSNYII"
     gene            complement(66926..67456)
                     /gene="hslV"
                     /locus_tag="PKI50_00925"
     CDS             complement(66926..67456)
                     /gene="hslV"
                     /locus_tag="PKI50_00925"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNT02313.1"
                     /translation="MFHGTTIAAVKRNDKGAIAGDGQVTFSQSTIIKSTAKKVRTLFD
                     GKVLVGFAGSVSDAFTLSEKLEEKLEQHSGNLKRAAVELAKDWRRDKVLRQLEAMLIA
                     MDKETLLVISGSGEVIEPDDGVIAIGSGGNYALAAAKALIQNTDLPSHEVVRKSLEIA
                     ASICVFTNNNIIVEEL"
     gene            complement(67559..68869)
                     /gene="trmFO"
                     /locus_tag="PKI50_00930"
     CDS             complement(67559..68869)
                     /gene="trmFO"
                     /locus_tag="PKI50_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNT02314.1"
                     /translation="MKEKAVVIGAGLAGSEAAWQLAKRGIKVRLYEMRPVASTPAHHT
                     DKFGELVCSNSLRSNQLENAVGLLKEEMRRLGSLIMECADSKALPAGGALAVDREGFS
                     QEITDRITGNSNVEIIREEVTCIPENEYVIVATGPLTSEKLSFSIGRMMDSDYLYFYD
                     AAAPIITYESINKDKVFMASRYEKGDSDYINCPMSEEEYVNFHRELVNAETAPLKSFE
                     KEVVFEGCMPVETMAKRGLQTLLFGPLKPVGLTDPRSGKQPYAVVQLRQDNKEGTLYN
                     IVGFQTHLKWGEQKRVFGMIPGLENAEFVRYGVMHRNTFINSPKVMRPTLQFHNNDKL
                     FFAGQMTGVEGYVESASSGLVAGINMARIIDGREPVVFPETTAHGALCRYITDRTIKA
                     FQPMNVNFGIFPQPEYRIKNKKEKSSYYAERALKDLEKFIRERL"
     gene            complement(68872..70962)
                     /gene="topA"
                     /locus_tag="PKI50_00935"
     CDS             complement(68872..70962)
                     /gene="topA"
                     /locus_tag="PKI50_00935"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNT02315.1"
                     /translation="MKTSLIIVESPAKVKTIKKFLSSSYKVEASMGHIRDLPKSQLGI
                     DIENNFEPKYITIRGKGEITDKLRKEAKNCDKVYLATDPDREGEAISWHLANILNLNT
                     REVSRIEFNEITKSAVTKALKNPRKLNFNLIDAQQARRVLDRLVGYKISPLLWKKVKK
                     GLSAGRVQSVAVRLICDREDEIRTFVPREYWTLAAKLNDPDTRKSFDAKFFGTENEKI
                     EISSESEMEKILEALKGAKYTVKRIKTGEKRRNSPNPFITSTLQQEAYRKLGFTAKRT
                     MMVAQQLYEGVDIKGEGTLGLVTYIRTDSVRISEEAKKDAAGYIMNKYGKSFLGGEIK
                     QNKSGKKVQDAHEAIRPTSVYREPESIKDSLDKEQYKLYKLIWERYVASQMQAAVFDT
                     VTADIEANGYIFKAYGSILKFPGFMSVYKESSDEKEEEEISLPRLTEGQLLKLKKLEH
                     KQHFTEPPLRYTEATLVKILEEKGIGRPSTYAPIISTILARGYVIRDKKFLVPTDLGE
                     KVTGIMKEYFSDIVDAEFTANMEGKLDNIEEGEKDWVHVIREFYGPFEKTLKSAEEKV
                     GKIKIQDEVSDVVCEFCGRNMVYKQGRFGKFLACPGFPNCRNTKAIVESTGINCPNCT
                     GELIARRTKKGRKFYGCSKYPECSFVIWDEPVAEKCPECGSLIVKKYNKKGFEYKCIN
                     EACGYKRSDNNEVK"
     gene            complement(71019..72107)
                     /gene="dprA"
                     /locus_tag="PKI50_00940"
     CDS             complement(71019..72107)
                     /gene="dprA"
                     /locus_tag="PKI50_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNT02316.1"
                     /translation="MKDKKYLMWLSSIPGVGSKTCLRLVKHFSSAENVFHCSFSELMN
                     SGIVREKIAESIKADRNTEKVNEYFRIVKDNDIKVYTIFENEYPENLKHIYDPPPVLY
                     VKGELTAGDSIAISIVGSRKASDYGLKTAHRIALRLAESGITIVSGMAMGIDSAAHRG
                     AIAANGRTIAVFGCGLKYVYPAANFRLSKEIEKRGALISEYPFDTEAYPSQFPARNRI
                     ISGMSLGVIVVEAGEKSGSLITADFALEQGREVFAVPGNITSTNSKGTNELIKNGAKL
                     VSRIEDILEEFNIEPICREKSNTFDNRSCEISAEERLILELLHSCGGDRDEIAAASGL
                     QTGKAMAALTMLEVKGLIRQTGGNYHLV"
     gene            complement(72280..72711)
                     /locus_tag="PKI50_00945"
     CDS             complement(72280..72711)
                     /locus_tag="PKI50_00945"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNT02317.1"
                     /translation="MSTNERDICSVIEKLPVYGKLLIKLYKSADIRKGQKLLLSAGIA
                     YSVSPIELIPGIIPVAGQLDNLIVMLRCLRKVLRSSDEKARERFLKESGMTIEEIDED
                     IKISVDTLKSIGRGTVRIVTNTGKFAGYSVMRHIKEYKNKR"
     gene            complement(72735..74267)
                     /locus_tag="PKI50_00950"
     CDS             complement(72735..74267)
                     /locus_tag="PKI50_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNT02318.1"
                     /translation="MIYKTNTCALYGIEGHVIEVETDLSNGLPGFDIVGLPDAAVRES
                     RERVRAAIKNSGFLFPQKRITVNLAPADIRKEGSVFDLAIASGILRASMYITNEALEG
                     FAFLGELSLNGSVKGIKGVLPMCLSLKEKGFYKLILARDNADEAALVNGMEIYPINSI
                     TDMAEFLNGNRFITPHKVDLEAILRNDTAETYDFSEVRGQNAAKRALEIAAAGAHNIL
                     MLGPPGSGKTMLARRLPSILPDMSFEESLEVTKIYSIAGLLKDKLYLVTRRPFRAPHH
                     TMSAGSLIGGGRVPHPGEVSLAHYGVLFLDEMPEFQKNVLEVLRQPLEDECVTISRVG
                     GTITYPAKIMLICSINPCPCGYYGDESGRCNCSPNHIKGYLGRISGPLMDRIDLHIEV
                     PSVNFNDLSCSPKEESSAAMKKRINRARQLQLERYENEGIFFNSQLKPKHMTAYCSIG
                     KKEKELLRNAFDRFNLSARAYNRILKVARTIADLEESESIGIDHIAEAIQYRSLDRKY
                     IL"
     gene            complement(74505..75314)
                     /locus_tag="PKI50_00955"
     CDS             complement(74505..75314)
                     /locus_tag="PKI50_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT02319.1"
                     /translation="MKEIISSSSNNTLKYIKSLQMKKARDEYGKFLIEGEKLFWEALD
                     YNVPISMVLISQSYAESSKHQEYAAALTGRNIPLHYADDRVFKRVCETETPQGIIAVA
                     EKLKYDIDNIISKDKLSLILLHELRDPGNVGTIIRAADACGLDAVAISKGSADLYNGK
                     TIRATMGSLFHIPVIQSVDTAEIIDKLKQRGVVTIGADPHSSINCTELPYQMKSAIII
                     GNESQGISSEIKEMLDINTTIPMPGRAESLNAGIAASILMYELYVRKSLQS"
     gene            complement(75311..75979)
                     /locus_tag="PKI50_00960"
     CDS             complement(75311..75979)
                     /locus_tag="PKI50_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNT02320.1"
                     /translation="MKQFVVIGIGRFGGAVAETLSDLGHEVLVIDTDEEAIQKISDKV
                     THAVTADATDESVLKSLGARNFDVAVVAIGSDIQSSILITLMLKELGVRHVVAKAQNE
                     LHARVLMKIGADKIVFPEREMGERVAHNLISSSILDFIELSEEHSIIEYAVPESWKGK
                     ALRDINMRARYGVTVVAIRNMSNESINISPKADSEIKDGDIMIVIGHNDDLKKLERKT
                     EKEI"
     gene            complement(75997..77343)
                     /locus_tag="PKI50_00965"
     CDS             complement(75997..77343)
                     /locus_tag="PKI50_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNT02321.1"
                     /translation="MRKKITMTPTQIIVIGFASMILIGTLLLNMPMASRNGQPVGLIN
                     SLFTATSSVCVTGLVVVDTGTYWTAFGQVVILLLIQIGGLGFMTMSTLLSLLLGRRIT
                     LKERLLIQESLNQFDLEGLVRLTKNIIIATIIIEFIGAIVFTIAFIPDYGLRKGIAFG
                     VFHAVTSFCNAGFDLIGGFRSFTPYVDSCIININTMFLIIIGGLGFSVWMDIYKAIKE
                     RDMRNVTLHSKVVVSTTIILILTGALFIFIMELNNPDTIKKLSLSGKLLASFFQSVSP
                     RTAGFNTLDMAVLTTPTKLMTIILMVIGGSPGSTAGGIKTATAAILFLTVISVIKGKE
                     DTEVFGRHLPQYLVYRAMSVSFISLSLIVIVTMLLSITENADFMTVFFEAASAFGTVG
                     LTLGYSPNLSPFGKLIISMTMFAGRVGPLTIVLALARRAINNRGNVKYPEDRIMVG"
     gene            complement(77481..77840)
                     /gene="rplT"
                     /locus_tag="PKI50_00970"
     CDS             complement(77481..77840)
                     /gene="rplT"
                     /locus_tag="PKI50_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNT02322.1"
                     /translation="MARVKRAINAHKKRRKILKLAKGYVGARSKKYRIANETVMRALR
                     FAYIGRKLKKRDYRKLWIARINAAARNNGISYSKFINGLKLSGIEINRKMLSELAIND
                     AAAFTKLVDTVKEKLNA"
     gene            complement(77862..78059)
                     /gene="rpmI"
                     /locus_tag="PKI50_00975"
     CDS             complement(77862..78059)
                     /gene="rpmI"
                     /locus_tag="PKI50_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNT02323.1"
                     /translation="MPKQKTHRASAKRFSFTKTGKVKRACAYKSHILTKKTTKRTRKL
                     RKGAYTTATEAKMIRKLVPYK"
     gene            complement(78126..78641)
                     /gene="infC"
                     /locus_tag="PKI50_00980"
     CDS             complement(78126..78641)
                     /gene="infC"
                     /locus_tag="PKI50_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNT02324.1"
                     /translation="MSSNKDLQINEEIRDRELRIIDSNGDQLGIMSSRQALELAAERQ
                     LDLVKIAPQAKPPVCRIMDYGKFKFEQSKKEKEARRNQKIVNIKEIRFSPTIEEHDLN
                     VKAKNAQKFLQEGDKVKVTIRFRGREVDHVSFGEKALGRFYDIVKDYCVVEKPAKLEG
                     RNMIMILAPKQ"
     misc_feature    complement(78658..78769)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(78852..80261)
                     /gene="pepV"
                     /locus_tag="PKI50_00985"
     CDS             complement(78852..80261)
                     /gene="pepV"
                     /locus_tag="PKI50_00985"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HNT02325.1"
                     /translation="MKLEKIVESMKDDIIKAAQELVRIRSIEGNPEPGAPFGKEVRIC
                     LDRSLDICSKLGFKTENFDGFAGHAEYGEGDEIVGILVHVDIVPEGSGWSQNPYGGDV
                     VNDKIYGRGTIDDKGPAAAAIYALKAVADSGVKFNRRVRIIIGCDEESGRWGCMKHYF
                     KYAEAPMCGFSPDADFPIINSEMGILIFNLEKEFEPNEDSRCGGLRIKRIQGGNRVNM
                     VPDYCECELEMKKDFTDRVIKTVEYMKNEQNMKLEMEIRDDKCIINSYGISAHGATPG
                     KGVNAISQLISCICKLPLCVSEQTEYVNFLNSSIGMEIDGKSFGVAMNDEVSGNLVFN
                     LGTIDMSDKKGSVGINIRYPVTKTGKEVYDIIRRKSSEAGIKVIEGNGQDPLYVPADN
                     FMIKILQKVYENVTGQKADLISIGGGTYARAIKNAVAFGPLFPGKEETAHQKDEYIEV
                     DDLMKCTKMFAEAIYELVK"
     gene            complement(80521..81831)
                     /locus_tag="PKI50_00990"
     CDS             complement(80521..81831)
                     /locus_tag="PKI50_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02326.1"
                     /translation="MKKAKNMLGKQMPYLTDNIVPIIFIAFTLLGFALTDNVPINYFL
                     SELSSRFYRNAFIVLSLIIPVIAGLGLNFGIVIGAMSGQLAIIIVRYLSIEYLHLSGV
                     WELFFCIALALPIAAFIGFLTGRLYNKTRGQEMIASLIVGFFANGVYQFILLFATGVI
                     IPVRATHPMIKPDGVGVRMTVDMGELHYSLQNIMKTPFMWTVLVVSAVLLILVVALYL
                     TGRKRPDKGNSVIFIIKVLLLAATIAYSAYAIATNNNITKVKDVPTVTIIFIGLLCLF
                     TELIMKTKLGQDFRSVGQSQHIAQSNGINVDKTRIIAVIMSTVMAAWGQIFYLQDMGT
                     LNVYGAHTQIGMFSVAALLVGGASVRKATVSQALIGTILFNSMFIMSPEIGKAVFGNA
                     LLGEYFRTFMVYGIIGMALGIYVWNDNRKARITLEPIELNTPDN"
     gene            complement(81831..82913)
                     /locus_tag="PKI50_00995"
     CDS             complement(81831..82913)
                     /locus_tag="PKI50_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02327.1"
                     /translation="MLNNEDKQNNSVKKNLDIPIVKSIGLPTLIIAAFFLLVMITAGV
                     LKQSLPMLISDILRRFGMNGVLVLAMVPAIKSGTGPNFALPLGIICGLLAWTIGIEMG
                     FTGIVLLLITSIIATVFAAATGYLYGRLLNAVKGAEMTIATYTGFSIVALMSLVWLMA
                     PFKSKQMGWMMGKGLRETIKLDGVNAGEILNNFLSFEIIGVKIPTGLLLTFFAFCFLV
                     WLFFRTKTGVAIIAGGSNPEFAAASGIDIDKNRVIANILSTVLGALGIILYSQSYGFF
                     QLYSGPLMMAFPAVAGVLIGGATASKANISHVIIGTFLLQGLLTASMPVANQLFAGTD
                     LSEIMRVILQNGIILYALTQVKGGGK"
     gene            complement(82914..84512)
                     /locus_tag="PKI50_01000"
     CDS             complement(82914..84512)
                     /locus_tag="PKI50_01000"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNT02328.1"
                     /translation="MGDKLLTIKNISKEFYGNQVLKDINIEIKKGEIHALIGENGAGK
                     STLMNILFGMPVIHETGGYQGSIELDGKEVMFNSPHEAMFHGIGMVHQEFMLIPGFTI
                     TENIKINRELTNPNLVSKIFGKQYESLDMKRMNSDARKALDIMGMGIEEYTLVAGLPV
                     GHMQFIEIAREIDKTGIKLLVFDEPTAVLTESETQNLLAAIEKLAAQGIGIIFISHRL
                     EEIMQVSHRVTILRDGEHVVTKDTKDTNVYEIAELMVGRKVSLDRSDSDSRRIQDESV
                     LSIKNLEVKMPGEEVKGFDLEVKKGEIIGIGGLAGQGKLGVANGIMGIFPARGQVIFE
                     GRPLELNNPRGAIVAGISFLSEDRKGVGLLLDESIELNTVFTSMQIHKEFLYNLGFIK
                     VMDSQKIRNHTNKMIKDLDIRCTSSLQHAGSLSGGNQQKVCMARALTQKPKLLLVSEP
                     TRGIDIGAKKLILDLLKSLNEEQGMTIVMTSSELAELRSICDRIAIVCDGKVKGILSP
                     EASNAEFGLMMSGVHADSQMGGAE"
     gene            complement(84656..85846)
                     /locus_tag="PKI50_01005"
     CDS             complement(84656..85846)
                     /locus_tag="PKI50_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNT02329.1"
                     /translation="MKKALALMLAVVMLFSFTACAKPAEPAAAEPAASEAKPEEAKDN
                     WKIAIVTNTVSQNEEEYRSAEFVQKKYGKDKIVHELWPDNFMTEQEQMISVLTKLAAD
                     PDIKAVVINQAVPGTNPAIDKFLEARNKDEVLIVYASPQENAPDVSTRADVILQPDEL
                     SMGSAIPNQALKQGAKTFVHYSFPRHMSIPILAERRELMKAEAAKIGLQFVDATAPDP
                     TSEAGVTGAQQFILEDVPKMVKKYGKDTSFFSTNCSMQVPLIKAAFDAGAIYTQPCCP
                     SPYHGYPSALGLNVPEDIVGGLQDVIDQTTKIVEDAGLSGRFSTWPVPAAMMATIAGA
                     EYAVKWLNGETDGKFDKKVLAECMSDYAGMDISLNPYVENGVTYDNYLLFLVDFLTYG
                     KLPS"
     gene            complement(86041..86676)
                     /locus_tag="PKI50_01010"
     CDS             complement(86041..86676)
                     /locus_tag="PKI50_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT02330.1"
                     /translation="MKKNNTPEIVIIDDEPDILYTIKEICLYGGWEAATASNGRQGYE
                     LCRMHKPSLAIVDYHMPDWDGLTTVKRIRQTDNAVSILVLTVDERQETAERFIAAGAT
                     DFAIKPIKSPDLISRIKINLKINEIQKKYILDKQQIYLDKGISPATLSIILDYLKEQG
                     HGLTIDDIANGLNLAYQTVHRYVSYLLEEKRIEIVPIYGQLGRPKNKYKLL"
     gene            complement(86663..88078)
                     /locus_tag="PKI50_01015"
     CDS             complement(86663..88078)
                     /locus_tag="PKI50_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT02331.1"
                     /translation="MTASQRKINENILLKTIAAGGVLLIITGMLFPVFFQNTWFNTIM
                     KIREAITTGDSGHLILASASLNSLCSIQSTLLYMGSILLIFNTKLSSRLKSIDVFLVS
                     LVSIVFLHWMDSLIFEIPWEPVSTLLALIISLFLFEKLFVETYSFIQVFIVSVQVFFA
                     FQWLNIMPYFSSYRFGRSDVPYSVKISGIYLNADTVLNFAGFAFFIPFIISAFITATL
                     FISYSRNIHMMRENHIKENEIRDMRAKALENQIYQEVKSLVHDLKTPLVTIRGLNSLM
                     VSENANGKLAEYSERIENSVAKMSDMISSFLYEYSRQKLKASDLISYIRAQLPLEDEK
                     IRFSISIEEDLPDIYINKIRIARAVINVLENAIVVPCKHSYKMINFDVRRAEKGINII
                     IKDNGIGIDESNLTKIWEIGYSTNNTSGLGLPFAKRIIEDNAGTIEVGSQRDFGTTVT
                     IFLPSVDNFEDYGRGEAIEKK"
     gene            complement(88106..88705)
                     /locus_tag="PKI50_01020"
     CDS             complement(88106..88705)
                     /locus_tag="PKI50_01020"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNT02332.1"
                     /translation="MKRNVFRMVLIIILALLLVLVNMPYKKKAETIVFTLPGLPRPIA
                     KVPVVITSAGQSTDTYLINDISNQLMIRSFFMPQAEAEGIEDMNSIVFAVGYSSLGLK
                     LQGISYEDEKARIEKLINMAKDNKLTIITVVLGGEQPIDVRNEEIISLVGAKSDYIIG
                     LKGSSNEELLADLAENGNIPLTLVRKVKDISVPFASAFR"
     gene            complement(88725..90332)
                     /locus_tag="PKI50_01025"
     CDS             complement(88725..90332)
                     /locus_tag="PKI50_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT02333.1"
                     /translation="MKIGNKTLIVLSAFAIIIAVMSGTLKDAARGSYDTEKRLIVGRA
                     NDSVTLDPSCTVEMDSFKVTVNILETLVKCENGGNEVVPLLAESWESSEDGLKWVFKL
                     RRGVKFHDGTAFDADAVVLNFNRWMYEDNPYHKGSFNYWNYIFGGFPGLVKSITALNN
                     YSVEIKLNKPFAPFLYAMTMPAFGIASPEAIKKHQGDLSEHPVGTGPFVYRSWERNKS
                     IILERNDKYWNDAAKVERVEFKVIPGNKDRLEELRQGTIHIADYLSPNDIAGIKYDPN
                     LYLYFRPSFNVGYMAMNNEKSPFNNRLVRAAINHAVDKDKLINDVFDNLAKPAATFIP
                     PSLWGYNGSLEIYEYNPEKSRQLLAEAGYPNGFNTTIWVMNASREYFPDPLKTAQFIR
                     ENLRQVSINAEIVVFNWNEYLKKIRNGEHEIALIGWTGDYADPDNFLYTMLASENAKP
                     GLAGNYSFYKSEDVDQLLAQARQTTNMSFRRSLYRRIQETVNYDMPSVPMVHTMPVLA
                     ARLSVKGYTPHMTGIESLEQVSLENER"
     gene            complement(90480..92390)
                     /gene="thrS"
                     /locus_tag="PKI50_01030"
     CDS             complement(90480..92390)
                     /gene="thrS"
                     /locus_tag="PKI50_01030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNT02334.1"
                     /translation="MVKITLKDGSVKEYKKGTTVEEIVASIGSGLLKAALAAKLNGKV
                     ADLRTPVNEDCSLEVLTFDDDDGKWALRHTGSHILAQAVKRLYPDAKLAIGPAIDTGF
                     YYDIDSDKSFSEEDLAAIEKEMENIVKEDLRLERFELPRSEALDFMGVMGESYKVELI
                     NDLPEDAVISFYKQEEFVDLCAGPHAPSTGKVKALKLLSVAGAYWRGNEKNKMLQRIY
                     GTAYTKKSELEEYLKMLEEAKRRDHRKLGKELDLFSLHEEGPGFPFFHPKGMIIRNEL
                     ENFWRNIHRKHGYKEIKTPVILNEDLWHQSGHWDHYKDNMYFTTIDEKTYAVKPMNCP
                     GGILIYKNAMYSYRDLPLRMGELGLVHRHELSGALHGLMRVRNFTQDDAHLYLTPEQI
                     KEEVLGVISLTDYVYKLFGFKYRVELSTRPENSMGSDEDWENATEALRNALEASKLPY
                     KINEGDGAFYGPKIDFHLEDCIGRTWQCGTIQLDFQMPEKFNLTYIGADSEKHRPVMI
                     HRTIFGSIERFIGILIENYAGAFPVWLAPVQVIILPVSEKQRDYAEKLNKQLEDLEIR
                     AEADLRSEKVGYKIREAQLQKIPYMLVVGDKEMESGSVSVRDRKEGDAGVMKFEDFLS
                     KIQDEIKNKTNK"
     gene            complement(92721..93317)
                     /locus_tag="PKI50_01035"
     CDS             complement(92721..93317)
                     /locus_tag="PKI50_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNT02335.1"
                     /translation="MYEALFLIVVGAIIGWLTNVLAIKMLFRPIRPFRIPLLNITIQG
                     LIPKRRNDIAKSIGNVVEKELVSINEIFDRLVTEENKQEVIAIIKEKLLKVIDYKIPA
                     IIPYSIKSRIIDYFDEQINKDAMSILDSSVDGIISNTAYKVKIGEMVEEKIDELELEN
                     MERLILNLTSKELKYIELLGGLLGGIIGFVQTVIIRLL"
BASE COUNT        23647 a        22133 c        15876 g        31821 t
ORIGIN      
        1 ataaatatat ctcagccgtt aaaacagcag cacccattcc tatattcatg cacagaaata
       61 taagaacagc tatatcaaac aggtaaccaa cggcactgct aaacatcata attccatttg
      121 ccagtaacgc gcatttgatt attctctcta tcctggtatt gccaaatacc catgccgcaa
      181 aaatacacga taacccgaaa aaaatcgtcc acccaagcat attaacggca gctattccgg
      241 aaatggggtt tgcctggata aactgttcaa gtccgtttgt ttggccggaa ttaatctgca
      301 ttcgcaccgc acttatttgt ataaagtagt ttatgccaat caatgcaaaa aaacctatgc
      361 caaaaagctc tgctatcctg gcaaagtact tctttgatga ttcggcaatt tcctcaatgc
      421 tgcagagctg aatgacaaag catgcaccat agagaatcat aaaaagcatg gcaatatgct
      481 taaaggcttg attagtagtc tgagctgcct ttatgtaggt ttcaaagtta gtccatataa
      541 aaacaggatt tacgagcaga attgcaataa aacaaatggt atacgcgaca aaagccagtg
      601 cagctgttac tgctgaccag tacccgaaat tcaatgcatt cttattcatt catatatccc
      661 ctttactata tttttgtata ataaaaggag atcttcattc cagcttatgg gtagagagta
      721 ttatttcact gaatgggaaa aaacaaatcc caagaatcac aagaaaaacc atgtgcataa
      781 aaacatagta aatgctgtta tccggaataa ttgcatctgt cagaaaagaa acaaatgtta
      841 taagaaatat gaatatcatc agtaatgcaa ttacagctat tatacggttt ttcttcataa
      901 ttcatccgtc ctctaaacaa acttaattgg gaataattac attatagcac atcgcttcga
      961 ttatatcatg cccagcttat caattgcctg ctcttcatcg ggcaagaaaa atatattcct
     1021 tcctttattg ctttcataaa tgaagtcttt caggctctta cttgaataaa ccgagaaatc
     1081 gccgactatt gctattctaa tatggtaatt tataaacttc tgcaatacct cccctgcgaa
     1141 tcttgttttc aggtcaaaaa agtcctcgca tattgctgat ttattcaaaa caatatgatt
     1201 acagcctgtt tcataatcta tggctgctat gaaatctagt gctgattgaa catcttttat
     1261 cagtatctca ttactgatga caactgcaat ttctgtatcg ttttctttta ctactgttat
     1321 tttcatgata atcctccatt ataaatactt ataacatatt tcattatagc cggtataatt
     1381 attcttcttc caaaaggact gtgccaaata attgtctatt gatatcgttt ttgcattatc
     1441 tttcaaccta taaatatcat ttacatttac atgaaaaacc atcatctcat accataaatc
     1501 aataatattg cttaaatcat ttaaatcagc ttttctgacg agcattttaa tcactccata
     1561 aaataatttg acgttttcat acctcaattt ctatatcaat acattttgcg gcttcaatac
     1621 acaacttcat atcgttcaaa ccaatttttc ccttgtacaa tttcattgct tcatttttca
     1681 aagccacttt attatataaa tcttcagggg aagcttttga tactttttca accgtatcaa
     1741 atccggcggc aaatagtaca cgggcaaaag ttgtatttac ccattgtatt cttgttaaat
     1801 cacttagttt cagcaattca attatttcat gttcgtcaat accgaatgta ttaacaaatt
     1861 tttctctttc atatgatttc ccaagttttt catacaacat ttgagtattt gagattcctg
     1921 ctttttcaat tttatcaata gtattgtttg aaatccatgt aaaatctctt aatttcactg
     1981 gtttgggctg aatacttttt aactcccgaa gtaaaatcgt aagatattca tctgaaatat
     2041 tcttattttt attcaataat tctgctttta atttgctgtt ttttaagact gagaataatt
     2101 cttcaagtgt cttaatattc gcttttacaa acacattgaa atgtatatca gctttgtctt
     2161 tcagtatttg gcggcttggg atgaatgttc tcttctcaag ttccttttta tattgttcga
     2221 gtgtaatttt tttaaaatca attaaataac tcatttttat ccccctagtt aattttttgt
     2281 tccaaatatt ttatacaaca ttattagtat acagcaattt tctgttctga ttagatatgt
     2341 gttcattatt agattaccta agttagtaat ttccattata tcacctcata aattacacat
     2401 ctttattcaa tattgtatgc ggcaatgtct ataagctcag aatcattgca tttttccgta
     2461 cttaaaattc tttcatactt gtaccctttt tgacttaaag caatatctaa gaagcagatg
     2521 aaaattccca tgtcaattga attatagtaa gaaagtttac ccgccggcat gaatgatttt
     2581 atttgagttg ttcgataaat tctcaggcaa ttttcttcac tgacaactct ccacggctgt
     2641 gtattgcagg cacttggtgc atatcgaaca atttccccta tgtctgaata atgttcacct
     2701 ttccatatag tttctaaatc cttgcggttc gtttttgaaa aatccttacg aaaatcttca
     2761 ggatgacttt ttccaaatgc caacattata acatattcaa gaccgtcgag ttttagttct
     2821 ttggttttgg ctaaagcata ccagcagact cctatattac gggaagccaa aaacaagtcc
     2881 atctgctcca acatatagcc gacattcaga agatataacg gcttttcttc gctatacata
     2941 agcaagcagt attcaccgcg ttttgaggtt gtttctttac tttccactat tttaaattta
     3001 actttgatat ctttaacaag aggcctgaaa ttatttattg cctgcctgat atcttccatt
     3061 tcagcttctg ttatagataa gttataatca tatcttctta ttgattttct tttaaaaatc
     3121 atgtcaaata gttcattcaa aatataatcc ccttttaatt cggttctctt acttaaatgg
     3181 taatggatta taaaatttat tccaacatgt attttaaaaa ttgcaatgca atattaattg
     3241 accctacaaa caagggcatg gtatttaatt atgtatgtaa tcttactatc cgatttatct
     3301 aaatatatac cggtatattc tatctataat aaaaatcaaa tgataatccg caaaaatggc
     3361 attctgcttc ataccagctg tgttctgctc cacattgaca tttccatttt aattcttgtt
     3421 tcttgagcca cttctcgcaa ccttgttctt ttaattcagc gagattgtcc attatatcca
     3481 aatgatgagc tcgcccatct aattgaaaat tcctcaacat ttcacaagga gcttctatac
     3541 aatcaccaca gtactcaatt cctttatctt cagcgcattc tcttatttta cattttgaac
     3601 aatgggggta taacttatcg gagcggcatc cctgacatgt gggatcttcg actgttttat
     3661 cttttttaag taattctcgc aataaatgtg catttcgggg aaaagaagcc tgataatgat
     3721 aacacgctcc acaatataaa ccacatactc ctaacaattt ataactcaac aaaaagacct
     3781 cctaacattt ttaataataa ttcaaaaact atcaatagcc ttccggatac aggcacatat
     3841 tgcttttcga acatgtcgtt ctaacgtggc gcgggctttg cgcccgctgc gtgaagccat
     3901 tgttaggcga tgggcagagt atgttcttag cttctttaat tatttttaac cccgcccatt
     3961 ctgctgaagc gcaggacgcg cgtaagcggt ttctaaaaac catacttatc ttttctaagt
     4021 ttaaatgctt tttctccacc ctccataatc tttgtaataa cgtccctaat aattgatata
     4081 tctattgcac caagatgctt tatttcacca ttagactgat ctcctgaaac tctataatca
     4141 caacaagcta ctagttcagc gtcaccattg acaacaatat tcgcattatg gcttgtaaaa
     4201 atcagttgac ggtttttctt tgatttgcat attagttcag ctatttcgga aaccatctga
     4261 ttatctaaat catcttctgg ttggtcaatt attagtgttg ggccttcttg gttgagtaaa
     4321 acatgaataa gtgatgttgc ttgctgacct gcagaggcat cagtaaattc aatatattcg
     4381 ccttcctttg ttttgtattc aaaaactgga atatcttcta gttgggtaag atacatattt
     4441 atgaaattgt tgatattcat tttttctgca atttttctca aatcattttg tgataagcca
     4501 atagaaacta atttcggagt cgaaggaaag gtctcagggg aatcattttc aaaatcgaaa
     4561 tttgcaaggc tctcaaactc atttaaaagt tcttgccatt cattaatggg attggttgcg
     4621 tttgaaacaa atgaacatat tgaatcagtc ttttctttcc tgatatttga ccctgccaaa
     4681 atactcctta atgtcgaacc aagtgagtct atacccttgc ctctcccaag ggttgcgcgt
     4741 aaattattat ctgataagat ggataattgc tcacattgag cctggatagt atcagctcta
     4801 ccattatgcg aagaaaccca ttgttctttt agtcttgaaa atttttgatc ttggtcgccc
     4861 aatttgttta ataattgctt tttttcccca agggatttgc gaatttcatt agatcttttt
     4921 tcgagttggg atatttgtgt taaagttgct tgctgagaag aagatcgctc ttttgctgat
     4981 tcataatgct gtttatgtat taagagaagt tgcatccaat cagttcttcc atgcgagaat
     5041 tcattttctg cggcactata ttcggctata ttcttcttga tatcgtttaa ttgtttttta
     5101 acatctttaa atagttgaga aatactatca ttgatggtct tgatggtatc ctttgaagca
     5161 tccggaatta taagtctcgg tggaatgggt gtaggaaaag cttcaatttc agtcaaaagt
     5221 acatccaaag aattggatgc agctgttagt tccgacaccc attcctcaac taattgttct
     5281 gtttcagaat aggcttcgtg agtatcaatt gtagctctat cttcatctga aataccttta
     5341 agattttttc taagattttc tacctgctca gttacagatt tgagttcaag ttctctgcgg
     5401 tgtatctctt tttgtaatag atttatttga tttcttttct caaaacaagc cctaatatcg
     5461 gtattaaggt tcttaaaatg caagtcatac tcagaaaggt tctgcctaat ggggctatac
     5521 acgagtcgct tcaattctgt tccactaaca ccaacactac taagttgttt ttggctatac
     5581 gcttgaatcg gtaatagatt tcttatattt tcttcagtac atacctcaaa atcttcattt
     5641 cctattttta gcaaaatttc cccagatttt gcttttctgc gaacaacatg tacaataccg
     5701 tttttaataa aggaaacctg aactgttccg tcataaggta aaagcgttct ttcgattaac
     5761 ttttttcgtc tttcctgata attcggaagt tcttcgtcct cctgtgataa atcaggatat
     5821 tggtcacata aaccccacct gagatattcc aaaacagtag atttacccgt tcctcttcct
     5881 ccaataatgg cattgtattg gcgattgaat tcaaggtaaa ttcgacctag aaacttactg
     5941 tttgatacat ccagtgaagt aatgaataca ggcggtagaa caggtatctc ttgagaaata
     6001 cgggagtttt ttgcaagaca ggcttgtcgt agcgcttccg cagttggctc agcccacttt
     6061 atccaagtac tatgtctacc aaggtcttca tgtgtcgcct tgcgattatc agacgtttga
     6121 attagtgcaa tttctttgaa accataatgc ctgttttttc cattaactat gtccgtattc
     6181 cctcgtccaa gagtagaaat ggaactctct aaataaccgc caacgcaagg cattgtttta
     6241 taaaaatcag caaaaccgct tcgtaataat gttttgtggc cattatcact gacatgaggg
     6301 ataatagtga atctgccctt aatataatca agattactta gtttttcgta taattcacag
     6361 aaattttttg tgacagcttg cggtatcgga acgattgttg cagttcttga ttcagtgtca
     6421 ggatttggtg taattcctag tgtggttagt actccctgta ataagttttc tggaaaagca
     6481 ctgtcaagaa ttaataacgc ttggcaaggt ggggatgcaa gagttaattc taatccagga
     6541 aaaactatta gtttttcctc atcgggataa cacaccccgt tatcatttaa ttcagctaaa
     6601 gcggcttgtt taatataagg gaagaaaaca aaatcatgat gatcagttat ggcaattgca
     6661 tgtaatcctt ttgtgcgaca agcttgtact aaagtttcag catatgcctt tctttcctca
     6721 tttgaaatcg cttcgggtcc atgccaatta atatcacgcg gtgaatgtac ttgaaagtca
     6781 catctataaa agtgagcccc cttatccatt ctatcctctc ctcctttttg cccgccatgg
     6841 acggcgggcg tcttaggcgg ggttaaaaaa tacaccctta atgtttataa atctattatc
     6901 ctaaactatt ttatcgctct gcctttcgcc taacgtggcg cgggatttac gacgttcacg
     6961 agccctagtg cgaccgcact tggcgagtga atgtgcgtaa aggctgctgt taggcgatgt
     7021 ggcgcttcgc cactgcctaa cagcgggcgc aaagcctgcg ccacgttaga gcgaatggag
     7081 cgaagcgaca tgtcgctcta actcctgcaa ctacgtatgt cgtcaataca tccaaaatcg
     7141 tgtgtgttca cgaacatatt tcgtcaatat tttcatttat ctgcgaatcc actctatata
     7201 aattctccat atttcgagat aaatccttcc aaataaatac aaaataagta tgagtccatc
     7261 atacttatct cagatatgtt cttcagttgt gctgcaaaag aaaaaacata aaccccttgg
     7321 tttatgcttt ttctttttct tcttctgcct tctcttcttc ttttgccaca tcggccattg
     7381 cgtgccgtct tgaaaggtta attcttccct gcttgtcgat ttcggttact tttacaagta
     7441 tctcgtctcc gactgcgaca acatcctcaa ccttttcaac tcgttttgta tcaagcttgg
     7501 agatgtgtac gaggccttcc ttgccgggaa gtatctcaac aaatgctccg aaggtcataa
     7561 gcctggtaac tctgccgaga tagatttctc cttcttttac ttcctttaca aggctttcga
     7621 ttattttcag cgccttgtca ccggctgcct gatttacagc agcgatgaat actcttccgt
     7681 catcctcaat gtctattttt acgcccgtct ctgcgataat cttgttgatt gtctttccgc
     7741 cggggcctat tatgtctctt atcttgtcag gatcaaccct tgttgttatt atttttggtg
     7801 cataaggtga cagttctttt ctgggttcgc ttattacgct gagcattttg tcaagtataa
     7861 agagccttcc cactcttgcc tgctccagtg ccctttcaag tataggcctg tcaataccgg
     7921 ctatttttat gtccatctga atggcagtga ttcccttgct tgttcctgcg accttgaaat
     7981 ccatgtctcc caggaaatcc tccatgccct gtatatcggt aagaatggct acattgccct
     8041 tctccttcat aagccccatt gcaactccgg ctacaggtgc ctttatcggt acccctgcat
     8101 ccatcaatgc caatgtgctg ccgcagactg agccttgtga tgtggaaccg ttggagctta
     8161 ctacttcgga tacaagcctg attgtataag ggaattcttc ttctgatggt atcattggct
     8221 ccaatgctct ttcagcaagg gctccgtgtc ctatttccct tcttcccggg cctctcatga
     8281 atcttgtctc acctacgctg tatggcggga aattgtagtg atgcatgtat ctttttgatt
     8341 cttcttcggt aaggccgtca agtatctgta catcacccaa agcgccaaga gtcgcaattg
     8401 taagaacctg tgtctggcct cttgtaaaaa gccctgaacc gtgtactctg ggaagaactc
     8461 caacctcgct gcttatgggc cttatttcgt caagctttct atcgtcgggt cttatgccct
     8521 cctctgttat cagctttctt acctgttcct tgagtatgtc atacaacacc tcgtcaatgt
     8581 ccttttcttt ctcggggaag atttctgcaa aatgctcaaa ggtttcttct ttaactgcat
     8641 caattttctc ctgtctttca agcttatcgg gagtccttat cgcatcaagg gtcttctggg
     8701 ttgcatattc ccttacggca gtttcaattt cttcttccgg cttgaaaaca gtgtattctt
     8761 ttttctcctt gcctacttta gcaacaattt cttcaataaa tgcaacaatc tttttgatat
     8821 tgtcatgagc aaacattatt gcttcaagca tgacatcctc aggtacttcc tttgcacctg
     8881 cctcaaccat catgacagca tccttggtgc ctgatactac caagtgaagg tcgctttttt
     8941 ctctttcttc gttatttgga ttgagtacta actctccgtc aactcttcca accaataccg
     9001 agcctgtagg cccctggaag ggtattgttg aaatggaaag cgcagctgat gaacctatca
     9061 ttgctgctat ttcaggcagg ttatcctgat ctacggataa aacggtagcc actacctgca
     9121 cgtcgttcct gtatccgtgc ggaaacaaag gcctgatcgg tctgtcaatc aatcttgctg
     9181 acagtattgc tttttcggta ggccttcctt ccctttttat gaagccgccc ggaatcttac
     9241 cgactgcata aagtctttct tcatagtcaa cgctgagagg gaaaaaatct attccttcct
     9301 ttggctctgc cgaagcgcat gctgttacaa gtacgacagt atcgccgtat cttaccagga
     9361 ccgctgcatc ggctaactgg gctattcttc ctgtttcaat acttagcttt ctcccagcca
     9421 gctcaagttc aaatacatcc atgtattttc ctcctttcat tttgtatatt aagtataatc
     9481 tgaaaccggg caaaaacttt tcctgttcca gaaaacttac tccatatgta tttagtgtat
     9541 ccatgtcaaa tcaaaataat tgcaaaaagg acgggaatga tccccgccct caaactacta
     9601 ttttcttaga cctagttttt caataatagc acggtatctc tcaatgtctt tagccttgag
     9661 ataattcaga agtcctcttc tctgtccgac catcttaaga agccctcttc ttgagtgatg
     9721 atccttcttg ttgatctgaa gatgttcatt caggttgttt attctctctg taagtattgc
     9781 aatctgtact tcaggagatc ctgtatcacc ttctttgatc tgatacttct gaatcagttc
     9841 gccttttgat tcctttgtaa gtgccattaa ttccacctcc ttataaatcc cgaatatatc
     9901 tccaaaagcc aagccaaccg ttggtgaatc ggtacaccta gctattggca tcattttgac
     9961 aagtatgatt ttaacatata tagttctccg tgtaaaggat atttattcta gcatcatcta
    10021 tgattcaaat gtgtatctgt cctgaatgtc gcggtttatc tgctcgacaa gtccttctat
    10081 actgtcaaac ttcatttcat ccctcttatg cttgtggaag aaaatctcaa tttcctcccc
    10141 gtaaatatcg tcgcaaaagt cgagaagatg cgtctccaca ctcaggcttt caccgttaaa
    10201 ggtcgggtta aagcctatat ttgttatgct tttaaaaagc cttccgcata cttttgtact
    10261 ggtataataa actccctttt taggaagtgt tatatccttc ttaacgtcta tattggcagt
    10321 tttgaaaccc attaactcac cattttgttt accgtaaaca acttcaccgt gtactgaaaa
    10381 atccctgcca agatattgtg acactttgtc aacatttccg cttcttatta cgtgcctgat
    10441 gaggctgctt gaaactatat ggccgtttac ctcaaaggca tcaactatga ttaccctgaa
    10501 gccgtatttt tcgccgtatt gcttaagatc atcgacattg ccctctcctt tgataccaaa
    10561 tttgtagttg tatcctatta caacggattt tacactaaaa cggcttacaa gtatgtcttt
    10621 gacaaaatcc tcggctctca tttccctgat tttgttgaaa tcctcaaggt acagcatgtc
    10681 aacgcccatc tgctcaatta cctcagaccg ctttttattg tcggtaatca gataaatgtg
    10741 cttattgggt atgagaattt tccttggatg tgtatgaaag gtatatacca tgcttcctgt
    10801 accgtgaagc ctcgattctt cttttaatat ctcaatcagc tttctatgcc ctatatgaac
    10861 accgtcgaag cttcccaatg caatgcacca cggcgctgac ttcatagctt catcatactg
    10921 ttctgctatt ttcaagataa ccacccttat aacaagattt caataagggt ttttgcacgt
    10981 ttgcaaaaac ctgcccgagc gtttcaacga ggcgggaaat atactcgccg ccttttggag
    11041 gaacatttta ttcgcctcgt tatttacaag aattaaatga atatattttt tatttttata
    11101 aatagcctct gtccgctttc atcattctgc acagtgcctg ttcctataaa accggcttct
    11161 ccgtatatgc ggatgtcctc gttttcatta agcccttcaa aaccgttcag cactccttgt
    11221 tcatacaggg gattgccgtt tgttatggat ttaagggcgg aagcttttac agtaattgat
    11281 ttataatgct tgaacaggat ttccacatta ataagaatgt catttatccg gttttcttca
    11341 gccgctgctt taatctcctc cagggtatag gaatcctcaa tcgaaaaggg agaggagcca
    11401 agcctgataa gctgtgacat gtgtgcgccg cagccgaggg cttcgcctat atcatggcaa
    11461 aggcttctga tataggtgcc ttttgagcat aatatatcaa taatagcttc atcttccgat
    11521 atcatttcca ccagcttaag ctcatgtatt tccacaggcc tttcggctat ccggatttct
    11581 ttgccttcaa gggcatattg gtaaagcttc ttccctttta tttttattgc ggaatatacg
    11641 ggaggcttct ggctgattgc tcctgtaaag cttttcaagg tgtcttccaa agcttttttg
    11701 tcgattacca ccgattcata ttctttgatg acctgtccgt agctgtcaaa ggtatcggtg
    11761 acagtgccga atttgatatt ggccctgtag cttttttgct tgtcagtcag gtattggact
    11821 gccttcgtgg cctttcccac gcagacagga agcactccgg cggcttcagg gtccaaagtt
    11881 ccggtatggc ctaccttctt tatattcagt actttcctta aataggacac cacatcatga
    11941 gaggtcattc ccggcggctt caaaacattt atgattccgt tcataatggc tccctacctt
    12001 aattcctctg cgactgcttc cagtatcttt tctttcactt cggcaattgt gccgtgaact
    12061 gtgcatcctg ccgcatgcac atggccgccg cccccaaaac ggagagccac ggctgctacg
    12121 tcggctttct tttttgacct cagtcctact tttattattc cgggctcgat ttctttgaga
    12181 aacatggcta cctctacaca gccaatatct cttgcaaggt ttattatgcc gtctgtatcc
    12241 tcttccaatg catttgcctc tttcatctgc tccaatgaaa ggcttatgca ggatgcggca
    12301 tcattgtgaa agaactccag agtgctgagg gctgtcttca taagcattac cttttccttt
    12361 gagttgttct ggtatattct ttcaaacata aggggcacac tgaccccgtt gtctattagg
    12421 tctcctgcaa tttggtgcgt tacggaggtt gtattgctgt attggaattg acccgtatct
    12481 gttacaattg cagtgtacag gcattcgcat atctccctgt ccatgccgat atccagagac
    12541 tttaccagtt catatattat ctcccctgaa gcggctgaac cggtatctac aaggtttaaa
    12601 ctgccgaact ccttgttgct tatatgatgg tctatattga ttacgtaatt gtcattaagg
    12661 taaagactgg atttacccag tctttcagca tctccgcaat caagggctat taccgcatca
    12721 aagttttcga attgaccggg cttccgaacc tgctcggctc cttccaaaaa agcatatatc
    12781 tttggaattt catcatcaag aatgaatctt acgtcctttc cgcatttctt cagggcatta
    12841 taaagtgcaa ggctggatcc gatactgtca ccgtccggca ttatatgaga taatatggca
    12901 attttactgc tctccttgat tttgcttatt atgttttgaa aaatcaaagg ttctctcctc
    12961 cgtccttttt ctttacctca ttcaagagct tgtttatgta tgctccatgt tcaatggatc
    13021 tgtcaagctc aaaaatcacc tccggcaaat atcggagttc aatcttcctt cccaattcac
    13081 gccggataaa tcctgcggcg ttggtaaggc ccttcattgt agccttcttt tcttcatcac
    13141 ttccaagaat gcttacaaat acctttgcat atctcaaatc agcggttact tctgctgata
    13201 ctacgctgca cattgttgat atccttgggt cattcaggtc gtttctgatt atatcactga
    13261 ctaccctttt cacttcttcg gatattctgt ctattctatg attagccacc gttaatcacc
    13321 tgcctgtata ttttggatat gttattttct cggaactgcc tctgtcgtat aggactcaat
    13381 aatgtcccct tctttaatgt cattaaactt ctctatgttc aagccgcatt cgtatcctgc
    13441 attgacttct tttgcatcat ccttgaatct cttcagggat gccagctttc cttcgtgtat
    13501 tacgatgccg tcgcggataa ccctgatatc gttatttctg tttattttac cgtcagtgac
    13561 atagcatcct gctattgtgc ctactgcaga agctttaaag gtggcgcgga cttccgcgtg
    13621 tcccaataac acttctttat attcgggctc cagcataccc ttcatagcag cttctacatc
    13681 ctcaattgca ttgtaaatta ccctatacag cctgatatcg accttttcct tttcggcaag
    13741 ggcaatggcc ataggctgcg gcctgacatt gaatcctata attatagcgt ttgaagcgga
    13801 agcaagcatt acatcggatt cggttattgc acctacaccg ccatgtattg tattcacttt
    13861 tacttcttca ttgcttaacc ttgcaagaga ttgcctcaca gcttccacag aaccctgcac
    13921 atcagctttt agtatgatgt taagctcctt gactttgcct tcttgtatct gattgaagag
    13981 ctcctcaaga gacactgtct gcttgctctg tatctgtgaa agacgctgct tttcctttcg
    14041 tttttccgaa atagtctttg ctgtcttttc atcatccaca acaatgaata tgtcccccgc
    14101 atcaggaact tcatcaagcc cttgtatttc cacaggtgtt gaagggcctg cttttttgat
    14161 tctctttcct ttatcgtcta tcatgcccct tacttttccg tgagtgcttc caactatgaa
    14221 gtaatctcca aggctcaagg agccgtcttg aataagcaca gttgcaacag ggccttttcc
    14281 tttgtccagc tctgcttcta ttactgtacc ctttcctttt ttgttgggat tggcttttaa
    14341 ttcctgcatt tcggcaacaa gcaatatcat ttcaagaagt tccgatatgc cttccctttt
    14401 ctttgccgaa accggaacca ttatagtatc gcctccccat tcctctgaaa caaggccgta
    14461 ttcagtcaat tcctgcttta ctctgtcggg attggctccg ggtttgtcaa ttttgtttat
    14521 tgctataatc aatgctacat ctgccgcttt cgcatggttt attgcttcta ccgtctgcgg
    14581 cattacgccg tcatccgctg caacgaccaa tactgcaata tcggtaacct ttgcccccct
    14641 ggccctcatt gccgtaaagg cttcgtggcc cggagtgtcc aggaatgcta tttttctgtc
    14701 accgatagtt actgtatacg cccctatatg ctgtgttatt ccgccagcct ccgttgcaat
    14761 tacattagtc tgtcttattg catccagcaa ggacgtcttt ccgtggtcta catgccccat
    14821 gatagttaca acaggagggc gcggcctcaa atcctctggc ctgtcttctt cagtcttgag
    14881 aagcacttct tcgctttcct tgtcaataac cttatctacg gtaaaaccga attcttcaca
    14941 tatcagctgc gccgtatcaa aatccagcat ttgattaatg tttgcaatga tacccaacag
    15001 caggagcttt ttgattattt ctgtcacagg ctttccaagt ctttcgctaa gttccttcag
    15061 agatatcata tcgcctattt taacaggctt ctttgagaca tggacttctt ctttttttgg
    15121 tgcctcaaag ccccgctgct taaaattctt attgtagggc tttccctgct gctgttgcct
    15181 gaacggaggt tttccctgag gggatttgcc cgggagtttc tttcctattc tttcaacttt
    15241 tgtaaaatct ttttttgtgc taagcgttat tgcatcttct tctatttcat catgagcgga
    15301 tacgttcttg ggagcactga ccggccttat atcagaagac ggatttcttt tatctcctga
    15361 ggggtgctga tgaggtttac ccgccggcct gatgtccttt ttttgttccg gcctcacagg
    15421 ctgctgcgca gactgaggcc tttgctgcgg ctgctgcact gtctgcggct tctgctgcgg
    15481 tttctgagcc tgcggaggct gctgtggctg ctgaagcttc tgctgaggtt tttgaacctg
    15541 ttgaggctgc tgcactgtct gcggcgcccg ctgagccttt tgcgcctgtt gaggctgctg
    15601 cgcagtctgc ggtgccctct gagccttctg ttcctgttga ggctgctgtg ccgcctgcgg
    15661 tttctgctga gtttgctgag gctgctgagg tttctgagga agagtcctct tcttgggctg
    15721 ttgagcttga attggctttg gggcttcaac attatcatct tttttaagat gcttcttaat
    15781 tgcggatgct tctgaatcct ccagactgct catatggtta ttaacattta tattcagctc
    15841 tttggctgcc gcaatcaatt ctttgctttg aactccaagt tcctttgcca attcgtatat
    15901 tcttatttta ttcaataaaa ccacccccag gtttttcttt ttttctcact tcatcaataa
    15961 gcttggcttt gattccattg ctgaagcttt cgtcagttat tcccagtacc gatcttgcac
    16021 tctttcccaa actgatgccg aattcatcct tcaatcccca tactataaaa ggtatactgt
    16081 agaattctgc tgaattgctc atacgcttct ttgtattatc agaggcatcc tctgaaatta
    16141 ttaccagctt gactctgccg tttcttattg agtttctcac tgcatcttcg cctgtaacca
    16201 gtttgcctgc tttattggca aggcttatca tcgaatatgc cttattcatg ggaaagctgc
    16261 gtccttaatc tattgaatac atcttcatct accggtgctt ccagtgcatt ttccaccctc
    16321 tttgccttaa tggcagcttc caggcacgtc aggtcgggac atatatagca gcctcttccc
    16381 ggtgcttttc caaccttatc tatatccacc tttccttcgg ggcttcttac tatcctgatt
    16441 agttcccttt taggtttcat ttcgttacag ccgagacatt ttctcagagg tacttttctt
    16501 tttctttgca tagaatctgc ctcctttaaa taccataggc ctgtgattga ctctttatgt
    16561 caattttcca gcctgtaagc ttggcagcca gccttgcatt ctgtccttcc ttgccgatgg
    16621 cgagagagag ctgtgaatct cctacgacaa ctcttgcggc atttgtttcc ttgtctgcaa
    16681 atacttctat tacttttgca gggcttaatg cactgcctac aaatatctca ggatcattgc
    16741 tccactgaat gatgtctatt ttctctccct taagctcgtc aactattact tgaactctat
    16801 ggcccctttg ccctacacag gctccgaccg ggtcaatatt ctcatcgtgg gaatatacgg
    16861 caatttttgt tctggatcct gcttctcttg ctatgctttt tatttctaca attccgcttt
    16921 gtatttccgg aacctccagc tcaaaaagtc tcttcaccaa accgggatgc gttcttgaaa
    16981 gtattatctg cgggcctttt gtggtctttt ttacttccac aatataggtt tttatcctgt
    17041 cacctatatt atacacttca ttcggggttt gttccccggg ccccaatatg gcttcggttt
    17101 tgcccaggtc aatgaaaaca ctgttctttt ccttcctctg cacaagtcct gttactattt
    17161 cattttccct gcttatatat tcatcaaata tgatgcctct ttccgcctcc cgaatcctct
    17221 gcaccactac ctgtttggct gtctgagccg caattcttcc gaaattcttc ggtgtgacct
    17281 tcgtttcaac tacatttcct gcttcataat tcttatctat ttcttttgca tcctgaaggc
    17341 ttatttgcat aaattcatta tcgacctctt caaccactgt cttaagtgcg taaacgtcaa
    17401 cctctccggt ttccctgttt atttgaacct tgacattctg tgaggttcca aagtttttct
    17461 tgaaggccga aaccagcgca acatcaattg catctatgag cagttccttt gaaataccct
    17521 tttcctttgc tatttgttcc agtgcctgga gaaactctcc attcatttaa tgctccctcc
    17581 tataaatata ttaaaacttt attaaaggcc gtattaaggc aattgaggcc ttttctacat
    17641 ttaaagcctt accttcaact tcaatttcta ctgaactttc agtatgccct ttaaggatac
    17701 cttcaataat cttcttgccc tccaaggggg aaaacagttt aatctcaatc ggctttccga
    17761 tattcttcct atagtctctg tccgtcttca gaggcctatc caggccggga gaagagactt
    17821 caaatatata tgactgttct atcggatcgg ctatatcaag caaatcgctg agttcttcac
    17881 tgacagactg gcagtcatct atcgttattc ctccttcttt gtctatgtaa actctgagat
    17941 accagtccgg cccttctttt ttgtactcta tttctacaag ctcgaagttg tttctgtcaa
    18001 ttataggctt tgccagctcg cagacaatat cttcaacttt tcgttttgcc aaggcgtcca
    18061 ctctcctttt atgtgatatt ctgagtaatt tttcttatat taatacgaaa gagtgggatt
    18121 tgcccactct ggtaataaag tcattaataa atactttgct gcatactcgc aggaaaacat
    18181 tgtttcccta tccattacaa aattatagca ctcttttata ttacatgcaa gttttatgtt
    18241 atttatactc caaggtttcc gcctgctgag aacaggctta gctggttgct ttcaggaagc
    18301 ccttcaaggc atccgtgatt cttgagtatt tctattactg tcttggataa ccttgccctt
    18361 tcccgaagat catccatgga tatgaagcat gattcctgcc ttgcgcacgc tatattctgc
    18421 gcggcgcttg cgcccactcc ctgaagggaa gccaatgggg gcagaatccc ttcctttgta
    18481 accataaatc tttttacatc ggatttataa atatccactg gcaaaagctt tatacctctg
    18541 agatacattt cataaacaac ctctataagg gtcataaggt ttttttcctt tgcagttgca
    18601 ttgttcccca atttatcgat ttctatccat tttgccctta tggattcctt gccctttatg
    18661 aacaaatcag catcgaaatc ctccagtttt actgtataat aagtggcata gaaagcctcg
    18721 ggatagtaca ccttgtaata tgccaccctg aaggacatca tgacatatgc cgtggcatga
    18781 gctttcggaa acaggtactt gactttcttg caggattcta tgtaccattc gggaatattc
    18841 tgttccctca taatctcttc gtattcggga gtcaaacctt tgcctttcct tacattttcc
    18901 gcaatcttga aggaggttct tgggtttact cccttgtata tcaggtacag cattatatca
    18961 tctcttgtgg caataacttc cttaagggtt gcggtaccgc tttttatcag ttcctgtgca
    19021 ttgttgagcc aaacatcggt gccgtgggaa agccctgaaa tcctcaccag ctccgcaaag
    19081 gtgccgggtt gtgtgtccat aagcatctgc cgaacgaatt ttgttccgaa ttccggtatt
    19141 ccaaggcttc cgaccttgca ttcaatatct tcaaggctta ttcccagtgc ctccgcagat
    19201 gtaaacaggc tcattgtcct ggaatcatca aagggtatat tattaactcc gaagcctgtt
    19261 atgtcctcaa gcattcttat gattgacgga acatcatggc ccaggatatc cagcttaagc
    19321 agcctcccgc ttatggaatg atagtcaaag tgtgtggtta ttatattgga gcttgggtca
    19381 tcagccggcc tctgaatggg agtaaagtta tatatatcgg tgtaatccgg tactatcatg
    19441 actcctcccg ggtgctgccc cgttgtcctc tttatcccgg tacagccctt tatgagtctg
    19501 ttggcttcag cattgtttat ttttattcct ttttcttcat agtacttttt tatgaagccg
    19561 taagccgttt tttccgctat tgttccgatg gtgcccgccc tgaaaacatt ccccttcccg
    19621 aataatcctt cagtgtattt atgagcttca ggctggtatt ctccggcaaa gttcaggtca
    19681 atatcaggtt ccttgtctcc ctcaaaaccc aggaacatct caaaaggtat gtcatggccc
    19741 tcttttctga gtactgttcc gcattggggg caattatcgt cattaaggtc agcccctgat
    19801 tctattgatc cgtcaaggaa aaaaagggag tacttgcatt tcgggcacaa ataatggggg
    19861 ggcagcgggt ttacctcggt aatatcacac attgtagcaa caaaggaaga gcctacggag
    19921 cctcttgagc ctaccaggta accgtccgcc attgactttg caacaagctt ttgcgctata
    19981 aggtacataa cggcgtaacc gttgtttatt attgagttaa gttccttatc aagccgcttt
    20041 tcaacaacct cgggaagtat ttcgccataa agctcatggg cttttttcat ggtcatttcc
    20101 ctaatctgct cttctgctcc ctcgattttg ggcggatatg tattctccgg tataggaatt
    20161 atctcctcaa tctgttccag tattctgttg gggttcctca ccactaccga ataggcggtt
    20221 tccctgccga gatattcaaa ctcctccatc atttcatcgg ttgttttaaa atgcaacggt
    20281 gcctgctcat cagcatcaga gtagccctgg cctgccatga ttatgcgccg gtatatctca
    20341 tcctccggtt caagaaaatg aacatcccct gtagcaacta ccattttgtt cagcttcttg
    20401 cccagttcaa caatccgtct gtttatagct tcaagctctc ctttatttgc agcaattccg
    20461 cttcttacga gaaattcgtt gttgccgttg ggttgtattt ccagatagtc atagaatctg
    20521 gcgttttctt ctattgtatt ttcatccagg ttgttcagta tattcctgaa cacttcaccc
    20581 gattcacagg cggaaccgat taacaagcct tctcggtact gcataagcag gcttttgggc
    20641 agccgcggct ttttataaaa gaagtccagg tgggacatcg atatcagttt gtacaggttc
    20701 ctcaggcctg tcatattctt taccaggaga attatatgat aggattcacc tgtcttatgg
    20761 cttcttcctg acagagagag cttgtttata tcagctgcgt catttattcc tttgctcttc
    20821 atcatttcaa taaacctgag cattatctct gcggttgctc ttgcgtcatc ggcagctctg
    20881 tgatggtttt ccagctcaat acccaggtat tcgcatacaa tatccagctt atgcctcttt
    20941 aatccgccaa gcagcaccct tgacatctgg agggtatcga ttattgtatt attaaagatt
    21001 aagcccaatt ccgccgcctt tgccctgata aaaccgctgt caaagtttgc attatgcgca
    21061 actaacggtg aatcaccagc aaactcaagg aatttcggca atatttccct gattgtgggt
    21121 ttgtctttaa ccattgagtc cgttattccg gttagttttg taatattttc gggaatcgga
    21181 atttcgggat tgataagttc tgaaaacctg tccgttatcc tgccctgttt tattttaacc
    21241 gcgccaattt cggttatttt gtcttttatg ggataaaggc cggtagtctc aatatcaaat
    21301 actgtaaatt cttcttcgaa gcttctgttt acagcattgt aaactatcgg tactccatca
    21361 tttacaaagt agccttcaac tccgtatatt accttgactc catggatttt tgacgcttca
    21421 taggcctccg gatatgcctg aaccactccg tggtctgtaa ttgcaattgc cttatgcccc
    21481 caggattttg cctttttaat gagatccgat gcggaacaga tgccgtccat ggcgctcatt
    21541 cccgtatggg catgaagctc aactcttttt acttccgcat tatccgtgcg tattgatttt
    21601 tccgctgccg taatgctgat tgggaatact accaattctt taatgaactt gtcatactgg
    21661 atatcccctt ttaaggcaat atacctgccg tcgatgatgt ttgaagtcaa tttggcgaaa
    21721 tcattttctt cctttgcaaa aattttggcc gttattgaat ttgtattgtc cgttatattg
    21781 aaggttataa ttgttctgcc gttattgagt gtttttattt ccgtgttgaa tatgcagccc
    21841 tggattgtaa ctcttccgga atcctcggtt attgaagcga tgctgaccgg agtgtctttg
    21901 aaaggcttgc ccattatcag ttcatccttg ttctcctttg ctttgccgct gttgtcgctt
    21961 tttatgggct tcaaggcttc ggttattatt ttgttctcct cttcaaaata acacttcaga
    22021 tattcatcct cgcagtactt ttcttcatta tcggcacata ttgatacatg caggctgata
    22081 ttgaaatagt cattaatata ccgttcaata atatcattgc atttttttgc ggtgaggtat
    22141 tttgcagctt cctccgattt taccgtgatt ctgatgacgt tattttcaag ctgtacattt
    22201 gaatccataa taaaacccgt aaaagacggg ttgcttgcaa taactgcttt ttttaagtcc
    22261 tcccagtatg atttaacaag ctcatctgcg cttaagctga cagatgactc atcaatgatt
    22321 attcgtacct tgttcagttt aaagtattct gctatggctg attcaatttc tccaagctcg
    22381 ggaataggaa taaagccctt taagcttatt atcatttccc attcccttgc ttttttcaga
    22441 acccttattt ttttcagttc gaacttgtcc ttatattgaa gaaaaagatt cgagtccggt
    22501 ttcatatcat tttgcatatt gacctccgaa ggctgccgcc tatttactgc tgcctgattt
    22561 tatattatag ctgcttagaa tctttacaat atcccagtac attttcaggc gcgcatgcac
    22621 accccttata attccaagct tttcttcctt tgtaacatga gtcatgtcag gaaggtctac
    22681 ctcctgaacc ctgacattga atttatcgac atattttgtt aaagcaacct caacaccata
    22741 tctggtaata tctatattcg gtattttatc aatgattgat ttcttaattg ccctctgtcc
    22801 cgaaaggtat ggcgttacct tttgagccag atccgtcacc attcttccgt tcttaaatat
    22861 tcctattgtc atatctgcct tgttgtcaat taccggttct atgagatcca aaacatgctt
    22921 ttccgtaaga cctataaggt cggcatccag aaacaatatt atttctgttc cgcattcgtt
    22981 aatacctgct ctgattgcac cgcctttgcc tacattctca tcaagctcaa ttacatccgc
    23041 accgcattct cttgcaattt ctgcagtact gtcggtggag ccgtcgctga ccactattac
    23101 cttctgaatt ttgtcaatgc tttttacaca ctttactacg tctgcaatcg tctgttcttc
    23161 gttataagca ggaattaccg ctgttacatt cattgcgttt cctccttttg gtactacgca
    23221 ttattctgtc atagcatgca aaaattatac ggattgtaaa gcttctatct cctttatcaa
    23281 ttcttctatg acattttcct gaggtatctt cctgactgtt ttgcctttca ggaataacag
    23341 gaactcccct tgccccccgg caatacccaa atcagcttct tttgcctctc ccgggccgtt
    23401 taccgcacag cccataattg caaccttcat aggcttctta gcttctattc ctttaattct
    23461 tttttcaacc tcttccgtga ttctgataag atcaacacag gttcttccgc aagtggggca
    23521 ggatattatc tctataccct ctttaagcag cttcaggctc ttgagtatct cttttgccgc
    23581 atatatttcc tctgcggggt ctcctgtcag ggaaaccctt atggtatccc ctataccgtt
    23641 gtaaagcagt atacccagtc caaccgatga ttttacggaa cccgcaagca atgtccccgc
    23701 ctcggttata cccaggtgaa gagggtagtt ggcggccttg gatgctttta tatagctgtc
    23761 tattgtcagc agcacatctg aagatttaat ggaaattact atatcactaa agtccataga
    23821 ctcaacaagc cttacatttt tcaaagcgct ttccaccagc gcatcggctg tggggcttcc
    23881 gtacttttca agaatctctt tttccaggga acctgaatta atgccgattc tgatggggat
    23941 acccctttcc ttggctgcat caataacttt ttttaccctt tcattgtccc caatattccc
    24001 cggatttatc cttattttgt cagcgccgtt gataattgat tgtattgcca gcctatagtc
    24061 aaaatgaata tcggcaacca agggtattga tattttgttt ttaatatctt ttactgcttc
    24121 tgcggcttcc atatcgggaa ctgcgattct tataatatca cagcctgcag cttccagcct
    24181 cttaatctgt tctatggtgc ccgctttgtc ctgagtcctg gtatttgtca tagactgtat
    24241 aaggattggc tctccgcctc ctatatatct gttacctatt cttattttcc ttgtatatgt
    24301 gcccataatc cgccctacct gaaaatatcc attaaattga atcttataat atccctgtac
    24361 ataaggaata ttgaaagagc catcagtgct ataaggccta caaaatgcac cataccttcc
    24421 ttttcggcag gaaggggttt tcttctgata agctcaataa acaggaataa cagccttcct
    24481 ccgtcaaggg cgggcaatgg aagcaggtta attataccaa ggttcaggct tattaaggct
    24541 gtaaggtaaa gcacttttat gaaacctgcc ttggcagctt catttatgta tacaagtatt
    24601 gaaatgggcc cgctcacttc atttgcatca gctttgccta caaccagctg tcccagaaaa
    24661 ctcagcatca tttttgatac ggcataagtt gtatttattc cttctttaac ggaaaatccc
    24721 gaaataccaa ccttctgagt gatgcctatt acaatatcat ccgtgccggg gttcttaacc
    24781 ggagtaagca ttacttccct ttgaactccg tttttctcaa ccgttacggt tatctgttcg
    24841 ccttcatgct ttgctatttc aggcttcatt tctttccatt catttatctt tacgtcattt
    24901 ataccgacta tcatttctcc ggccttaata cctgacgttt cagcaggcat tccttttgtc
    24961 acctgcccga ctatcggagc agccaccggg gcaattacca gtgaatatat gagtattgtc
    25021 aaaatgatat tgaaaaccgg accggatata acagaagata tttttgaaat tatagggctg
    25081 ttattaaagg cccccggttc attattctct tcatcctctc ccagcatcag gcaaaatccc
    25141 cctatgggga atgcccttag tgaatacact gtttctccga attttttgcc gataattttc
    25201 ggccccatac ccagtgcgaa ttctacaacc tttatgccgc cgagacgtgc ggcggcgaaa
    25261 tgccccgcct catgtacaaa tactattaaa ttaaatacaa gtatcgcagc aattaacgtc
    25321 attacttaca ccccgtttca tacaaatagg ccagtgtctc tcttgaccag cggtcaagct
    25381 ctgagacttc ttctatatcc atattctcaa ttattttatg tttatcaaga gtcttttcta
    25441 taaggtttcc tatatccagg aagctgattt tgcccgagag gaataatttt accgcttctt
    25501 cattggcgcc gtttaatacc acacaggcgc tgtctcccat atccagagcc ctgaatgcca
    25561 gcctcaggca tttgaatttc tccgtatccg gcttgtaaaa ggtcaactct ccaactgagg
    25621 tcaggctgag ttttgcactc ttcatcggca gcctttccgg atatgtcagg gcgtattgaa
    25681 tgggaacttt catatcgggc agtcccagtt gtgcaataac tgcgccgtcc acaaattcta
    25741 ccgccgaatg gataatgctt tggggatgaa cgcatacttc tattgattcg ggcattatac
    25801 caaacagcca tctggcttct atgacttcaa gccccttgtt catgagagtt gctgaatcta
    25861 ttgttatctt cctgcccatg gaccaattgg gatgcttcaa tgcttcctgc accgtaacgc
    25921 tctccaattc cttcagggtc ttgttcctga aaggcccccc tgaagctgta agtattattt
    25981 ttgatatttc attatgcctg ctgccggcaa gacattgaaa tattgcagaa tgctcactgt
    26041 caaccggaag catgttcaca ttattctttt taacttcccg ggttataatc ctgcctgcgg
    26101 taacaagggt ttccttattt gcaagtgcta tattcttacc tgtttttatg gcattatatg
    26161 taggaatgag ccctgctata ccaacaatcg ccgaaactac ggtatcgcag ctttccaaag
    26221 ttgctgcttc caccactcct tcaaggcctg cggcaacaca agtcattcct cccacacggc
    26281 ttttaaattc tttgtatttt gacatgtctc ctatggcagc aatctttggc ctgaattccc
    26341 ttacctgggc ttccagcaag tcaatattcg aattggctgc cagtgcatct attttgaatt
    26401 cttccttatt ttctcttata atatccaagg tctgtgtgcc tattgagcct gtagacccca
    26461 gaactgataa tactttcact atatcaaatc cccaatcaga acgaaataat aataaatgat
    26521 cggcgcggta aagagaatgc tgtcgaacct gtccagtatt ccgccgtgcc ccggaattat
    26581 gtttccaaaa tccttgactc ctgcatatct tttaatatat gaggcagcca agtcacctgt
    26641 ctctgaagca ataccgcaga gaaaacctgt tattataaaa tgaatcagcc ttatttcaat
    26701 tccgtaatta tttaatataa tacctgtgat tatactgaat gcaacagaac ccgcaattcc
    26761 gcccagagca ccttccacag tcttctttgg gctgatcgcg gggcacaatt ttctcctgcc
    26821 catatatatg cctatgaaat atgcgaaggt atcggtaaac catgcagtca ggaaaggaag
    26881 ccacaatatt atattgccgt acctgaggtt ttcgatgaaa agcaggtggc tgaaaagaaa
    26941 aggtatatat gctattccga aaatgtttat cgatatctca gtaaaactgt gttttttgct
    27001 tatgatttca tatataagca atgtgatgac agaaagcgca atcaaaaacc cgggcctgtt
    27061 aaaggcaatt ggaagaagaa acataatata gtacaaaata actatagtat atccgaatat
    27121 tttgtttgtg cttatattta tgttgctcag tgccctgtaa tattcaaata atcctgtcaa
    27181 agagactgcc gctgtaaaca ggtaaagata cagccctcct gaatacaata taaatattat
    27241 aagcggtata ccgagcagcg cacttaatac ccttgttttc agcattttta cctccactac
    27301 ttcaatgcac caaaacgcct gctcctgttt ttatattcca atatagcact gtgaagcacc
    27361 gcctcatcaa agtccggcca gagttggtct acaaaaagca gttcactgta tgccccctgc
    27421 catagcagga aattacttat acgctgttcg ccgcttgttc gtattatcaa gtcgggatca
    27481 ggatcattgc ttgtgtacaa ataatttgaa aacacttttt cgtcagcgga atccatgtca
    27541 agatttccgg cctccaagtc cttaataagg tttctgcagg ctctcagtat ttctgcccgg
    27601 cctccgtaat tcagcgctat gttgaattga agcccttcat tattctttgt aagctcgata
    27661 gcctcttcta tctcagttct cgtttggtca ggaagcatat caacctcacc caatattttt
    27721 atctttactt tattcttatg gagcgcattc acttctcttc tcatatattc caccagcaga
    27781 tccataagat agttcacttc atcctgcggc cggttccagt tttctgtcga aaaggcatat
    27841 acggtcaata tcttaattcc caagtcgctg cagctcttaa ccgtcttctt taaagcttcc
    27901 atgcctgctt tatgccccat tactcttggc aagccttttt tttgtgccca gcggccgttt
    27961 ccgtccatta ttatggctat atgtacagga agcttgccct tatcaatatc tccaagcatc
    28021 agagaacctc ctcaaaaaag tatccctcat ggtgagggaa actatttaaa acacgatgtc
    28081 gtaagtatta ttttgtcaca ctcagccttc tgcctgacaa caataggtga atttccccgt
    28141 ctttctttct ttttatcttc gaagggtgtt gatgttgaat caataatgat atcatgtttt
    28201 tcaccgatta tctgcagtgc atgatccagc gtatatccta ttaccagagg tatattgtcc
    28261 aatatgctct aaacctccaa aatttctttt tccttcgctt ctatagcttt atcagcttct
    28321 ttgataaact tgtccgttat tttctgtatg ctttcctctg attttttgag gtcgtcttca
    28381 gtaatgtctc cgtccttctt aagcttctta agatgttcat tggcatcccg ccgtatattc
    28441 ctaattgcta cctttgattc ttcaccaagc tttttaacca ctttcaccag ttcttttctt
    28501 ctttcctctg taagctgcgg tattacaagc cttatcatct taccgtcatt ggaggggtta
    28561 atacccagat cggattttag tatggctttt tcaatgacgg gaatagattt tgcatcccac
    28621 ggacttatag taagaactct gggttccggt gcagcaatgt ttgccagttg attcaaaggt
    28681 gttggggctc catagtattc aacagtaatt ctgtccagaa gacttggatt tgctcgtccc
    28741 gctttaaggg tagaaagttc atgcttaaac acggtgaggg tcttttccat tttctcctga
    28801 atgttctttt cgatatcctt tatcataccg ttaatcctcc tttactactg tacctatctt
    28861 ttcccccata attgccttaa ttatattatc ggggtttttc aagtcaaata caagtatggg
    28921 aattttatta tcacggcata aggatgcagc cgtgctatcc attacgccca atcctttgtt
    28981 gattacttcc atatatgaaa gcttgtcata ttttcttgct tctttattta taacggggtc
    29041 actgtcatat acaccatcaa ctttttttgc cataaggatt atatccgctt ctatttccgc
    29101 tgcccttaga gcggcagtag tatcagtgga gaagtaagcg ttgccgattc cggatgcaaa
    29161 tattactacc cttccctttt caagatgcct gactgctctt cttctgatat agggttcagc
    29221 tatctgtctc atctcaattg ccgtctgaac gcgggtgggc acatttattc cttccaatgc
    29281 atcctgaagt gcaagggcgt ttatgacagt cgcaagcatt cccatatgat cggctgttgt
    29341 tctgtccatt ccctcactgg ttctgcccct ccagaaattg cctccgccga ctactatcgc
    29401 aacttccaca ttaagctccc ttaacagctt gactctttcc gcaacgtcca ggagcatgtc
    29461 atgatccaat ccgaatgact tttctccggc taaagcttca ccgcttattt taagtatcac
    29521 tctcttatat ttcacgtcac tcatatatat tatacctcca agtattttgc atcacggcat
    29581 gtataagcat aaatttctac aaattcaata aaaatccttc tgtttttcaa aaaaaagaga
    29641 acactaagtg ttccctattt attcacctga ctcattactt catcaacgaa gttgtcttct
    29701 ttcttctcaa ggccttcgcc tctttcaaac ctgacaaacc ttcttatcgt aatattttct
    29761 ccgatttttg ctatcagtga cgtaagtatt tcctgcactg tcttgtctgt atccttaatg
    29821 aagggctgtt ccagaaggca tatttccttg tagtacttct ctattctgcc ttcgaccatc
    29881 ctttcaataa ttttttcagg ttttccctca ttcaaagcct gggctttgta tatttcacgc
    29941 tctttctcta taaccttggg atcaacatct tcccttttga cgtataaagg attggaagca
    30001 gcaacctgca ttgccatgtc ctttacaaac tgtctgaatt cttcgttggc tgcaacaaag
    30061 tctgtttcgg aatttacttc aattagtact ccgattcttc cgcctccgtg tatgtatgag
    30121 tcaactattc cttcgcttgc tattctgcct gctttttttg cggcagcggc aagccctttt
    30181 tctcttagta tttctattgc tttctccatg tctccgttgc tttcggtaag agctttttta
    30241 cagtccatca tgcctgaacc ggttctttct cttaattcct taacagctga agctgaaatc
    30301 attttcttcg cctcctatga tatttatgaa aaaggcggct gtcgccgcct tatatactta
    30361 ccaagcagtt actcagcaag ctgttcacct tgtctagctt caataacagc atctgcaatt
    30421 tttgaagtaa gaagttttac ggccctaata gcatcgtcgt tgccgggaat cggatagtct
    30481 acttcttcag gatcacaatt ggtatcaact attgcaacta tcggaatccc gagaatgtgc
    30541 gcttcctgaa ttgcaatttt ttcttttctg gggtcaacca caaaaatcgc tcccggattc
    30601 tcacccatgt tcttgatacc gccaagattc ttctgaagct tttccttttc agctctcaga
    30661 ttgataactt ccttcttcgg aagtacttca aaagtgccgt cttcttccat tttctccagt
    30721 tcattaagtc ttacaattct ttttctgatg gtcttgaagt tggttagcat cccgcctagc
    30781 catctttggt tcacatagaa catattggct cttattgctt cagattcgat tgactcctgt
    30841 gcctgcttct tagttcctat gaaaagtata tccttgcctg aagctgccac ttcttttatg
    30901 aagttgtacg cttcttcgac cttctttact gttttctgaa gatcgatgat gtatatgccg
    30961 tttctttcag taaagatata ctccgccatc ttcggattcc atcttcttgt ctggtggccg
    31021 aagtgcactc ctgcttccaa tagctgtttc attgaaatta ctgacataat gtttcacctc
    31081 ctatagtttt tcctccatga ccgtcatatc ctttgaggac tttgtaaaag cactcccccg
    31141 aaggatcagg ccatgtgtgt ttttcacctt aaacagttta tcatataaca tatttattgg
    31201 caataatttt tttaaatcgg tgtctgatat ctcgtattta ttctatctaa ttatacaaaa
    31261 tttatgtaat gtcattttgt aagtatgctt attatttcat cataaccgga gcctttgggt
    31321 atctcaaaag tgccttcctt aagctttgtc tcattatttg tctctttaag cttatcaatg
    31381 aaagctcccg aatcattaat aatacccttc tgccggagaa gagcggctat actctcggaa
    31441 ctcataccgc ttttgatttc tacagtgacc ttctccgttg gagcagtatt ctcctgattc
    31501 cggctgtcta taatctcctc ttttgaaaaa acaacaaggc cgtgtttttg cgcctctttc
    31561 tttatttctt ccaatgtcag ttccttgttt cccatgctta tattcatcat tgatgcaagt
    31621 acaaggccta ttcccaaacc aagcattata tttttcaggt tatagttccc cattattcta
    31681 cctcattttt caaggacaat ataagcataa cctctccttg ccctatattc atctgctttg
    31741 caatatcggt aacactcatg ccctgagctg catattcaaa aacctttaac tgcttttgac
    31801 tgagcttttc atcaacaggc ttatccaaat cagaattgtc attgattatc tttttctttt
    31861 tatttgccgc gctgacattt ggtacgacag gggcagcagg cttattatct gccgatgagg
    31921 tgccgggaac ctgattattg aggctcttca attcttccct gaagctgctt aatttctctt
    31981 caatttcctg cactaccatt tctgatgtgt aattaagctc ggatattatt tcatcagcat
    32041 cttcaataac cttctttatc tcctgctctt taacgtccat gctgaaatat atatcttcgc
    32101 cctttacttt atcaagaaaa atcattattg aagcagccag tatgacaaat actccaatag
    32161 ccattaatac atagttaatc atctgagcac ctcgttcata tccttatatc tattgttgac
    32221 atggggtcct tgtttttctt attttcctgg ccttcattat cttcaggcct gctgtcctta
    32281 tgttcgccct tctgatcccc ttgccctctg ttttgcttcc catccctgtt aatcctggct
    32341 tcctgtgcct ttttcgtttc aattatctgc tgtgtgtttt tatcttgctg ctgttgttct
    32401 ttgtgcatat taatactgag attattcttt tccagttcgt tttctgcgtt ttttgccttc
    32461 tgtacctcag gcagctttgg tataattgtt tgtaagtcaa caggccgcaa agacattata
    32521 atcactccca attatttatc atatatcatt ataggaaagc atttttatct cagcatgcat
    32581 tctgcaaaag gaaacaaact ttatagaatc cttgatgtac atcatgctgc ttccgattat
    32641 tatcctgctg ccgggataaa taatatcata tacctttact tttccttttg ataattcttc
    32701 gaaataaacc tccaattcat tctgctcgtt taataactga tcttgcttct gcttaagctg
    32761 cagcttaagc tgtaaggatt ttgcctgaag tgccttttta tcctccggca gttccatact
    32821 tttgctcatt tttgtcagaa gatcaaccgc cttgcttgtt ttgtccaggt tatccgtaac
    32881 ctgcttcaat tcattcttta attcttcgaa tttatttctc atttcagggt tgacgccgac
    32941 ttccagttct gtaatcgtag ccatcggaga acctacaacc tttgctctta tttcttcgcc
    33001 taccttaaaa gtaccgccaa ccagcaagcc ttttctgccg gttgcctcca gcttctttcc
    33061 gcaggtaacc tggctgtgca ttattgcatc gctctggatg tttcctttta catctatctt
    33121 gctgttttct ataaacttgg ctacaagatc cccttcgcag taaagctctc ctctgttcat
    33181 accttgtatt cctctgtgaa gtattatatt gcctttggat tttatctgag ccccttcaac
    33241 aacaccataa acttccacat ctccttcggc ctcgattaaa aagcctgtca gaacattgcc
    33301 cattactatt acttttccgt taaaccttat gttgcctgta gagttatcca cattattctt
    33361 tacttcataa acggagaaaa catttacctt tccgtcaatg atttttactt caccgtcaat
    33421 tgctgagaca agctcaagtt cgtcttcgct tacaacaaca ttctttcccc ttggcaaggc
    33481 aagcttcttt ccatcctttc cgggaacatc ttcaccggaa acattctttc cgggtatgcc
    33541 ttcggtatgc ggcataaggg ttaccagctt ctctccggct ttgacatttt ttataaggga
    33601 cagttcatgg tagtcaattt ttccgtcttt gtctatacct attgactttt cggcggaagt
    33661 gtcaaaatgg tatacaagct ccgcatcctt ccccggctgc ggttctattc cttcagccac
    33721 aagtatttct ttgtcatata ccttttgaga aaccgccttt tcaatcacat cttctttaat
    33781 gttgaatttt acaccttcct gcttaagtgc ttcatttatt acttctaatg taatatcttt
    33841 gccgccgatg ggtttgctga taacaataaa agccttcatt ttgtccttag ccgtttttac
    33901 gaggcattca ctgtctatct tcttttccat ctggggctcg gctattttta tccaattatc
    33961 aaggcttccg atttccagca tttgttttat ttttgtcaga tccacgctct gaattcttcg
    34021 caggtcaatt tttgttacaa tatccactat tgtaacatct ttatccgctt taatatatac
    34081 tccgtcttct ctgaaatcaa aatctatact tttggtgttt gctttcagat tctcattcag
    34141 aatactgtca atattgctct caattatctc cagggtttta ttttccccct tcgctatctt
    34201 cactttgtag taagaggaaa acaggttctt tttgctttcc agaatctcaa tattgagttc
    34261 ttccttcctg gcattaaaat gctccattcc ttttgctatt aacttttcat aatccttgcc
    34321 ttcgagtatt atgtactcat tgctcattat tatcaccatc ctatcattca aatgcaattt
    34381 ttaacttgtt cttaagccga atgattgctt ttgtatgaag ttgtgaaact cttgattccg
    34441 aaatattcaa cagctttcct atctctttat atgtaagctc gtcatagtag tatagtgata
    34501 ctaccttttt ttctttttct ggtaaagcgc ttattgcatc tgcaagtatc tctttcattt
    34561 ccttatcttc aatcacattt tcaggagttt cggaatgctt gtccgcttca ttgctgataa
    34621 tttctctttt ctgttccagc aaatcatcca gggatactac agaataagtg gtgatattct
    34681 gtatcttctt ttggaattct tccaccgata atccaaagct ttccgccatc tcctcatcgg
    34741 tagcaggcct gccaagcctg ctttccaagt cattgtatga tttctccagt tctttggatt
    34801 tttgtcttat cgaacgggga agccagtcta tttccctcaa gtgatctata atagcacctc
    34861 ttattctaag ctgtgcataa gtttcaaatt taactcctct tgcaatatca tatttgtcaa
    34921 ttgcatctat taatccaaat ataccatagc tgacaaggtc atcaaattct acattgtttc
    34981 cgtaacttga atatagtctt cccgcaacat atttaactat atatgtatat ttaagtatta
    35041 attcctctct aatttccgca gctttcgtcc tggaatatac ttcccaaaga ttgaccattt
    35101 cggttattac ctccgtgcag tagtataagt taatccgtat tgctattgat gtatttgctc
    35161 aaatcggcag gattaacttc aataaattca ttgtcactgt cataatcgat tgcttccagt
    35221 gcttcatcat ccaaaggagg cacttttatg tccagtttac ttcccgttgc cgacaccgtt
    35281 ccgcctttat cctggatatg tatgctcact ctgtaatatt caataagatt caaaacagtt
    35341 tttgttatca atgaaccaaa tataccaaat actatggtaa cagccataca tcttatcatc
    35401 agttcggaaa aatctatatt gttgcatata cctagtgtta cagtaacaat aagtgcggtt
    35461 ataactatta aaattggtat aaaatcagat attctcatat tgaaacccca cattcacaat
    35521 accggacata tctaaatctg cttcgttcca tagcctatgg tttttattaa taatatacca
    35581 gtttctgaat ataattcaat agtacgccca tagttccctc cggtgtcctc cgaaataagc
    35641 ggaatattca acattttaag agtttccttt gtagctgtga catttcttaa acctattctc
    35701 attacttcac tggtatcatt gaaattgaac atctgcgccc ctccggctat cttactgaca
    35761 agcttgttcc gttttgcacc gatttttatc atttctgtaa ttaattttac aatacctgta
    35821 tctgcgaatt tcgcttcatt gctgttgttc tttatctgag cgctgctggg gagcataata
    35881 tgcaccatcc ctgcaatctt agttgaaggg tcatacaggc agattcctac acaagaacca
    35941 agccccaggg tagtaagcac tccgggacaa aatgtcgtat tcaaatctgc catcccaacc
    36001 ttaactatat cgatcatatt aaaatactcc caagctctta agaagaatct caaaagaatc
    36061 ctcgtcaggt attaaaaaga aattgctctt tacatggtat gtaccttcaa ataagtcagt
    36121 ttcaataagc attaccctgt cgccgacctg tccgaaaagt ataacgggta cgcttattat
    36181 tgctcctgcc atatctattg ccaaagaagg cacagacata gtcaggttca aatttgtcaa
    36241 agttgacaat gaggacagat aggaacccga aagtatgttc cccacttcct gcaatgctga
    36301 taaatccatc tcatccaggt catctccgtt gctatctctg cccataagca agtttgcaag
    36361 catttttgcc gattttttgt caaccagaac cataatgctc ccggtaatat ccccatttac
    36421 tttaaaataa atgccgacta cctccgtctc gggccctccg actaactccg caacattttt
    36481 aaactcaagg atatttacct tgggaacgtc catatcaatt ctctttgata tcatttttga
    36541 caaggctgtt gctgcatttc cggcaccgat gtttccgatt tcctttaaaa catcaatttg
    36601 caggctatta agttcttcaa ttgatattcc cattttactc acccttttta aacagccgga
    36661 ttagtgatta ttttcactat atccaacaat atgataagcc tctcacccac tttacctata
    36721 ccggatatat aaccttgatc cacgctgccg acaccatccg gtgcttcttc tatcgcatcg
    36781 ctgcttatct caaccacttc ggatgaataa tcggctgtaa accctacgat aatatcatcc
    36841 actgatacta ttattatacg ggtattgttg ctgtcttttc cttcgggcag gttgaatctt
    36901 ttgcgcaggt caattaccgg tatcacttct cctcttaggt taataacgcc tctcacataa
    36961 tgagcggtat tcggaactct tgtaatactc atcatttctt tgatttcttt taccctcagt
    37021 atgtcaattc catattcttc attctccaat ttgaatatta caaattgccg tgcaaatgtt
    37081 gctgtttcca aaagaaacac ctccttatat cagtgaattt gaatcaatta tcagtgcaac
    37141 ctgtccatcc ccaagtatag ttgcccctgc tatatttcta atgcctgaca agaactttcc
    37201 aagagttttt atgactattt cctgctgccc cataaggctg tccacaataa atgcagcatt
    37261 cttttcgcct ttttttacaa ctacggcagt caattgatcg ctgtcatcac tggatgcatt
    37321 tataacacca agcatctttg caatccttac tatcggcaga acattgcccc tgtttaatac
    37381 tacatcctgt ccatgtacat ttctgattgt agatgcttca atatcaatta tatcccttat
    37441 tacattgaga ggtattgcaa atttctcttc accgatattt acaagcagtg cctgtataat
    37501 tgcaagtgtt aaaggcaatc tgataataaa tttgcttcct tttcccctat agttctcaac
    37561 ctcaaccaat ccgttaagag attcgatttt tgtcttgaca acatccagtc ccactcctct
    37621 gccggaaagg cctgaaacct tgtctgcagt actgaagccg ggattgaaaa gcagttcaat
    37681 tgcacttttc tcatccaggt catctgcttc actgcttgtc aaaatgcctc tttctactgc
    37741 ctttcgtttt atcttttcaa tgtctattcc tgctccatcg tcttccactt ctataactac
    37801 gctgttgccg tcctgatatg cccttaggtt gactgttccg ttagccggct tgttattttt
    37861 aatcctttct tctttttctt caattccatg atcaatggag tttcttatta agtgtataag
    37921 cgggtcgcct atttcgtcta tgacagtcct gtccagttct gtatcttcac ccgacatgtt
    37981 aaggataata tccttggaaa ggctcttcgc aaggtctctt accattctgg ggaacctgtt
    38041 gaacactctt tctatgggaa ccattcttac tttcattact gcatcatgca ggtttgtagt
    38101 aatcctttca aggtattcca ctgcttctat catatcctga ttctttgaat cagttccaac
    38161 tccctcaagt ctggttttta taattatcag ttcgcttacc aggttcataa gcttatccag
    38221 cctgtcgata tccaccctta ctgtctttcc cattctggcc tttttgcctt gtatgtaatc
    38281 tccgttttct ttgtcttcaa ttgagtactt taatgtctcc ttttcttcat tagtaatctc
    38341 atcgatatct ttaacgtcta gttcttcagg aacttcaatc ctttcaaata tgacttcggt
    38401 tacttcggaa atggattcaa gctctttcct gatcaaagcc tcagtttctt tggtaattat
    38461 tgcaatggta aattcattat cgaatttttc gtcttcaata tcttcaacct tcggctctga
    38521 cttgaaaatc tccgcatatc tctcaactgt ctgaaatacc agaaaagctc ttgctgattt
    38581 cagcatgcag tctttggcca gcaccaccgt aattttatat actccatggt tctgctgcac
    38641 agccttcctg ataatattat catcatattg attaaacttc attttgcttt cctgtggttc
    38701 tttcttatcg gctgtgtcag atgcttctgc ttcaggcttt ttcgccagca taccattaag
    38761 gcctgcgata atagaattta cattactgct gctttctttt cctgtagcgg atatggtatt
    38821 tatggaatta ctaagataat caaggcattc aaacagaatg tcaatcatag aggaattcac
    38881 ttcaatatta ccggttctta ttgcatccag aacgttttcc atctgatgag tcagattagc
    38941 aatccctgta aatcccattg taccggccat accctttaat gtatgagcaa ctctgaagat
    39001 ctcattaagt gttttcatat cgccaggatt tgtttccagg ctcaacaggt tttgattcaa
    39061 ttcctgcaag tgttccaagg attcttcaat aaatatttcc aagtactgat tcacgtccaa
    39121 agtattacac ccccatgcat tttaatattt ctcccgtaat cccgtgtaaa ggagctatag
    39181 tatcgatagc gcctgtatta actgctgatt ttggcattcc gtaaaccacg caggttcttt
    39241 catcctgtgc aattgttttt cctcctgcat tctttatttc caaaatccct tcacttccgt
    39301 cacttcccat acctgtcata atgaccgcaa taattttgtc atgccctgtt tctgccactg
    39361 atttcatcat aacatttact gaaggcctga gtccattttc cggtgcctca ctgttcagaa
    39421 ggattttgta cttattatcc tcatggcttt ttacaagcat atgatagtta cccggagcta
    39481 cataagccca acctgacgtt agtacatcac cgtcctccgc ctcttttaca tttatctccg
    39541 acaagctatc caacctgttt gcaagggatt ttgtaaagcc cggaggcata tgctgtacta
    39601 taagtactgc ggcaggtata tcctcaggaa gctttgtcac gacctcctgg agcgctttgg
    39661 gccctcctgt ggaagtacct atggctacaa tgtatttcag gtattcatcg gatctgcttt
    39721 tatgtttaac cttcttgtaa acagaaggcg gcttgtattc tggttttatc tgagggaaat
    39781 accccgtctt tgaccagcat atggatttta ttttattgat taactcttcc ctcatctgtt
    39841 ccgcatcaat tgttattaat gatcgaaccg gttttacaac ataatcaaga gcacccaatt
    39901 caaatgcttt aatcgcagct tctccgcctt catagctaag gctggacagt attataacct
    39961 ttggtacagg cttaagcttc ataagctcaa caagagcctc aagcccattc attaccggca
    40021 tttcaatatc aagcgtgata atatccggct tcaatagtat tgctttttcc aacccttctt
    40081 tgccgtttcg tgctgtacct gctacttcaa tatcagcatc gtttttaagc atgtcggaaa
    40141 tcaatactct cataaatgcc gaatcgtcta cgataagaac tcttatcttt tctttcccgg
    40201 aaagcatatc aaattaaccc ctttttaatc aatctgcgct gttcttcaaa tataaactta
    40261 gtaattgcat tcctatccgc catatttaga tttatatact gcactccgta ggtatagaga
    40321 agcctgtagc tctcgttaag gattcggcgt acaattactc cctgcacaac cattattggt
    40381 ccgttaaggt cgagggcaaa ctccaatgta tcattttcat taaaatattc tcttgaactg
    40441 aacataagcc ctcctgcact tatgtcctgg atagtgcatt taaatttttc accatactgt
    40501 ttctcttcag ggttaatcac cagcctggcc tctatttctc tgaatatttt cagcctgtaa
    40561 tagtctcttc tctgtttttt gattacttca gaagccgcct tgagcctgag aagcggaata
    40621 cttccaaaaa gctttttaaa caatatggtt ctgatttcat aaattgcttc tcccttaaat
    40681 acgataagct tcagaacagt gccctgcctg atattcacat ccatgttctt atatatcggt
    40741 atcagtacat caaaaaattc ttcatactgg ccgtcatgaa tatcaactat ctggctcacg
    40801 tattgcctta attcccctac tgcatcaaaa tattctatta gtactttatc tcctatattg
    40861 aaatatttcg ttaagccctt cataataatt ctccttatga attataagtg caaatagatg
    40921 atatgtaaat tcatagctgc aattgatatg gcagattaat cagttgaata agctggtaag
    40981 tttgtttata aacattttaa ttcctgaaac agagctggga ctcaattctt tattatccat
    41041 caaagttaaa gcaatatcat tcatgttttt tgatgcctcg cttctggcaa agcttattac
    41101 aaatggctgc tgaattttga ctgcctttgt tacacaagta tcattcagta tatagccgag
    41161 tttttgcaat tttaccccaa ggaacttctg agttacaata tttaatttgt caaggatgtt
    41221 gaaagcctca gccgcattgt cagctctatt taccacaact ttgatatttt ttgttttatc
    41281 cttgtgggta atggttttta tcagagcata agcatccgtt atggaggtgg gctccggcgt
    41341 agttaccaat atgacctccc gtgcagcatg cacaaagctt ataacgtttt ctgaaagccc
    41401 tgctcccgta tcaattaata tgatatctgc ttcctcatcc agccttccga gcttcatcag
    41461 caaatccacc agctgagttc tgttcagttt caccaactct tgaactccgg aacctcccga
    41521 aacgaatttt actccttcat aaccttcttc aagtatttca agtatgttct tattgctgtt
    41581 tattacatcg gacaaattat atttggacat tttgcccatt acaacatcca cattggcaag
    41641 gcccaggtcc gcatcgatta ttaccaccct caggcccatc tgagcaaatt ttataccgag
    41701 gttcacagta aagtttgtct ttcctacacc gccttttccg cttgttactg caatcactct
    41761 tgaaccgggt ttatccgtaa gtatgcctgt ccttttctct tcgtctttat aattttttat
    41821 gctgcttatt atttctctta atctctcagc ttgatctttc atatagctta tctcccaaca
    41881 aacagtttat tatcttgccg ttatcggcaa tttcaatgtc atcaggcaca ctttgtccgg
    41941 tggtgatata tgaaatcggc ttcttagtga tataagcaac atttagtatc actccgaagg
    42001 ctgaggtttc atcaagtttt gtaaaaagca gcttatagtc atccagaaaa ctgtaattct
    42061 ttattatttc tatgcaatcg gaaaacttgg tggttgcact gattaccaaa tatacttcat
    42121 agttgatgct tgattttata agagatttta attccataag ctgatatttg tccttatggc
    42181 tcctgcctgc agtatcaaca agtatcagat ccttctcggc atagctttct atggcattaa
    42241 gaatctctcc cggagtgtat attatggata ggggtactcc gattatttca ctgtaggttt
    42301 taagctgctc aaccgctgct attctataag tatccgaagt aataagccct actctttttt
    42361 tctctgtcag aaccatattg gctgccagct ttgcaagagt agttgtttta cctacacctg
    42421 tagggcctat gactataatg acttttttcc ctccggcaaa tgaaaagggt tccggttctt
    42481 taacgtattt catcaaaata ttatagacag tatttcttac aattgcgtcg ctttgctgtt
    42541 tttcagagtc caaagtctcc ataagcttct ctttaatctc atttataatt accttgtcga
    42601 cctcattgtc ctcaagggtt ttaagatact tcttcactac atccgataat tttgtattct
    42661 cataatccac ctttacggcg tcataaacct tgttcagcat gtttttgagt tccatgaagt
    42721 cctgttgtat aagctcttta tcatcgagat gagtgtgttt ttttgtattg tcctttaatg
    42781 aattcttctg gacctgcaga ttctcattaa gttcttttat aaattcattt gtttcaatgg
    42841 tatgatcatt cctggcatta tttgtcctag cccctgaaga gatgtcatta tctattgttg
    42901 caactacttc aataagaggc tttgaaaaca aacctgccag gccttttttt ctgatcttcc
    42961 ttgtgtttag tattattgcg tctcttccca agtcggattt tactttctgc attgcttcct
    43021 gggctgtttc accaacatat ctttttactt tcattatata ctcaccaccc ctacggattg
    43081 tatctcaacc aatgggtcca attcattata tgacagcact attagtcccg gagcaacctg
    43141 ttcggtcagt ctcttgaagt acagtcttac aacaggcgaa gccagtatta tgggctgttc
    43201 tcccaatgat gtcagcttct gcatctcctt aagaagattg ttgattatct gctgcgctgt
    43261 atttggttca atgtttaaat atgaaccgta ctctgtctgc tgaactgaat ccattatggt
    43321 ttgttcaaga accgcatcca aggttattac cttggcctgc atttcaacta taaagcgttt
    43381 ggtaatattt cttctcattg actgcctcac gtattcggta agcatgtcgg tgtcttttgt
    43441 cattggtgca taatccgcaa gggtctccat aattgtaacc atatccctta tggatacgcc
    43501 ttccttcagc aggttggcaa gaaccttctg aatgtcgcca atgctaagct gcttgggaat
    43561 aacatcttcc accagtgccg gatagttttc tctcacattt tcaataattt tttgtacatc
    43621 ctgtctgcct aatagttcat gagcatgatt ccttatgatt tctgtaaggt gtgtggcaat
    43681 tacagaaggc ggatccacca ctgtatatcc catcatctct gcccgctcct tctggttttc
    43741 gctgatccac accgccggca ggccaaaagc cggttccgtg gtctttactc catccaattc
    43801 accgtcagca gtaccgggat tcattgccag ataattgtcg aacatcagtt ctccgcttgc
    43861 cacttcatta cctttaattt ttattacata atcattgggt ttaagttgta tattgtctct
    43921 cagccttatg accggcacta tcaaacccaa gtcaagagca cactgccttc ttatcattac
    43981 aactctgtct aaaaggtctc cgccctggtt cacatccgcc aagggtatga tcccgtatcc
    44041 gaactcaagt tctatcgggt ccacctgcag cagcgacata acattctcag gttttctgat
    44101 ctcttcgact tcaacatcct gggctttgat ttcctcttcc ttttgtgctg cttttatagt
    44161 ctttctgagg gtgtacccca tatacgccag tatggatgcc agcaaaatat tcggaacagg
    44221 aggcaatcct gtgaatgcca gtataagcaa tgcccctgat gcgatataaa gtacttttgg
    44281 ttctgataaa agctgtgcta tgatatcagt tcccaggtct gaatttgaag ccgcccttgt
    44341 tacaaccatg cctgccgcaa ttgaaacaag cagtgccgga atctgtgtaa ccaaaccgtc
    44401 tcctatagtc agattagtaa atgttgaaag ggattcgata aagtccaagt ccatttgcag
    44461 cattccaatt gcaaaaccgc ctacaatatt tattacagta attataatac ctgcaattgc
    44521 gtcacccttt acaaacttgc tggctccgtc catagctccg taaaagtcag cttcctgctg
    44581 tattgctttt cttctttcct tggcttccat ttcatttatc aggccggaat ttaaatccgc
    44641 gtctatggcc atttgcttgc ccggcattgc gtccagcgtg aatcttgcgg atacctcgga
    44701 aaccctttct gcaccctttg taataactat gtattgtact ataacaagta taagaaacat
    44761 tataaaacca actataggat tactgcctgt tacaaagctg ccaaaggcat ttataacttc
    44821 acctgcatat cccttgccca gtatcagcct tgttgtggag aaattaaggc acaatcggaa
    44881 cagcgtcgta atcagcagta ttgtcggaaa aatcgaaaat tgcagcggtt cctttatata
    44941 catggataac aggaaaataa ccagtgacaa tgaaatattc aatgtaagca gtatgtcaat
    45001 caaaatagta ggaaccggta ttactataag aattattaaa gctacaatca aaactgctat
    45061 tacatcacta tatctgccca tacataggtc tccttctatt tgtctttcaa actgtataca
    45121 tatgccagta cctctgccac tgccttgtac agttcactgg gtatctgctg tcctatttct
    45181 acgcttttat ataaggcttg agcaaggggt ttgttttcta ctatcggtac attgttcttt
    45241 tttgccgtat ccttgatttt ttctgcaata aggtcttttc cctttgctat taccaccggg
    45301 gcttccgaaa cagcctcttc atatttgacg gcaatagcaa aatgcgtagg gtttgttatt
    45361 attacatcgg cagacggaac ctctgccatc attcttctta gtgaaagctg cctctgcttt
    45421 tctttaattt ttgattttat ttgaggattc ccttcaataa gcttgtattc gtcttttatc
    45481 tcctgttttg acatccttat ctctttttca aaattccatt tctgaaagcc ataatcaaaa
    45541 accgagagga taagcagcac cgcagcagcc ctaatgccaa cgctgataat tgtatttccg
    45601 atgaatacca ttgtactttc aacattcata ttcagcaatt caggaattac cctgtaatta
    45661 tcaacgagat aatcataaat gatgaatcct ataatcccta ttttaaaaat agatttaagc
    45721 aattccacat aagcgttttt tgaaaacagc ctcttgaagc cttcaaccgg gttaatttta
    45781 ttcaaattcg gcattaaggg cttggctgtt aatacagaac caatctgaac atagttcatt
    45841 attattacgc aaagagcaac aatcagcagt attggcgcaa gtatctttgc aaatattaag
    45901 aggttttgca gaaatataag cataatttca ttagtggtaa acctggaatc aatatccttg
    45961 ggaaaactca gggaattcct tacggcaaat atcaaatcac cgaatataaa ttttgaaaga
    46021 aacctcatgc taaagaatga aatcaacagc actaccgctg tcactaactc cctgctcctt
    46081 ggaatctggc ccttctgccg cagctcctgt cttctctttg gagttgcagg ttcggtcttt
    46141 tctccgaaca gctgaagatt tatctttaaa taagcataat ctttcatcct ttagccatac
    46201 tccttattat caagtagaca tagccgtaca tcctgtcaaa ggtaaccgtg ataatctgta
    46261 taaacacagg aatcattatg taaagtgtaa taagtccgat accaattctc aagggaaggc
    46321 ctacaatgaa tacattcatc tgaggaacag ttcttgaaag tattccaagg gctacctcgg
    46381 caagcagagt agcagccatt accggaatgc ttattttgaa ccctatgaga aatacctctg
    46441 taaaaattgc agcaatatta tttgtcatag cttcggtaaa agcaaagccg tcaataggca
    46501 aaacgctata gcttttgtac agtgctgcca gcaatacatg atgcccgtca attgccagaa
    46561 aaagtattgt ggtcattata tatataaagt ttcctatcat aggaatctga gtattcgtgg
    46621 taggatcaag aacattaacc attccgaagc caacctgcat gtcgattatc tgtcctgcca
    46681 gataaagagc cgaaaaaacc ataaagctta caaatccgat aattattccc accagaaatt
    46741 ccttggctat aatcgctgaa aaactgaata tgttggaata atcaatccgt acatccccca
    46801 gcaaaggaac cattatgtag gaacagaaaa atgcaagtcc tattttcaaa taagccggca
    46861 tctctctcct tccgaatacc ggtgttatta cgaaaagaga tgtcattctt ataaaaacca
    46921 gcagcagtac caggaatctg tcgaccaaaa caacactaat actatccata atcatttaat
    46981 ataaacaggg atattctcaa acagccttac ggtaaactca tacattatgt tcatcatcca
    47041 tgggccaaag accagcatag tcaaggaaac agctattatc ttcggtataa acgataaagt
    47101 aggctcttgt atctgtgtag ttgcctgcac tatgctcact ataaggccga ccagaagccc
    47161 caatcccagt attggcgctg aaaccagcaa aacagatttt attgcttcct gtgcaacatc
    47221 aattactatt ccttgatcca tagattacac ctctattaat taaatcccaa aataagagat
    47281 cttactatca gattccagcc gtcaaccatt acaaagagta atattttaaa aggcattgaa
    47341 atcataatcg gcggcagcat catcataccc attgacataa gtgtacttga tacaatcata
    47401 tctatcatca aaaaaggtat atataagaaa aaaccaatct caaaggctgt tttaagctcg
    47461 cttataataa atgccggtat aagcgcagag gttggtatat cgatcttggc tacgggctgt
    47521 ttcacctggc ttagattcag gaacaatgca agatcctttt ctcttgtgta cttaaacata
    47581 aaattgcgta taggctctgc agccttttct attgcagcct cttgtgtaat ctcaccgttt
    47641 gtaaacggtg tgaatgcatt ttcattgatt tctgtaatta ccggtgacat tataaaaaag
    47701 gttagaaaca gcgccagccc tataagcact tgattaggcg gcatctgctg cgtacccagg
    47761 gcatttcttg taaacgaaag cacaattatt atcctggtaa aagaagtaag cataatcagt
    47821 attgacggtg caagggaaat aatagtaagt ataaaaagta tctgcatgct cagtgccaca
    47881 tcctgaggat tttcggcagt ttctatatca agtcctattt tgggtatcgg aagcggttcg
    47941 caccaaacat caataattcc cattaaaatc atcaatacca aaatcagtat aatttttata
    48001 ataccgtttt tcttatacat ctgttttgtt acctctaaca attgatttca gcttattcag
    48061 attattcttt attgagttca tcccgttttt tttgttttca tcctgaaagc tgctcatata
    48121 aacttcaata ctttcatcat ctccaacagc cagtttctcc gggtccacct ccgtaagcag
    48181 tgttatgctc ttctgtgtac ttcccagcag caggtattgt tctcctactt taaccagtaa
    48241 aatgctttta tcaataccaa gaggtgctgc ctccactatc ttaagattct tatttccggc
    48301 atttttgagg cttctttttg acaaaaacct tgtagcataa taagcggcag ccagaataac
    48361 agctgacatg aataggaaat ataatattcc gtatattgta ctgcctgtat tcataaaacc
    48421 tgctccttct tatcccaata ccttcttaac tgcctcaata atcctttctg cctggaaggg
    48481 cttaacaata aaatcttttg ctcctgcttg tattgcttct atgaccatag cctgctgtcc
    48541 catggcggaa cacataataa cctttgcggc actgtttatt ttttttattt cctttacggc
    48601 ttgtatcccg tcaacttcag gcatagtaat atccattata accaaatcag gattaatttc
    48661 cttgtacttt tctatagctt ttgcgccatt ctcagcctct ccgctgattt caaaaccgtt
    48721 ttttgacaat acatccttaa tcatcattct catgaatgcc gcgtcgtcaa caatcagtat
    48781 accgttagcc atttgattat ttcctccttg taaatgtaaa atattgacta ttgaattctg
    48841 ctaagacgct tcgatgcact tacaatatca gtgattctta caccaaagct ttcatcaatt
    48901 acgactactt cgcccttggc aatgtattta ccgtttacca ttatatccac aggttctcct
    48961 gcaagcttgt ccagctcaag aattgtaccc gggctgaact caagaatatc tcttatcaat
    49021 ttttttgtcc gccccagctc tactgttatt tcaagcggaa catccataat aagctcaatg
    49081 ctttcttttt cagcgttcaa tttttcgaca tcaaaggttt ggtattgtac gggctgaaca
    49141 ttgacaactt tacgttcctc tcttcttgca gcaggctcat actcctctct ttcttcatac
    49201 cctttgctaa gtattgtatt gctttccggc ttttgaggct gagtgccggg atcgctgaca
    49261 gccggctgct tcacttgagg ctcttcggct ttgcctacga atataggttc agactcggtt
    49321 gaaatgttat ccagaaggct tcttacaagc tcttttgcaa aatctatcgg tataagctgc
    49381 attatctcac tgtctattat atctcctact tccatcttaa aagcaatttt aaccacttta
    49441 tcggctgaat tgaactgctc aagttcgctt aagttctgaa agtataatat aaacgcacgg
    49501 ggagggtaaa tatctattct cttattgaac attgaagata acgaagttga ggatgaacca
    49561 atcatttggt tcatggcttc ccctatggca ctaagctgaa gatcgcctac cgaatcgggg
    49621 cttatctccc ccgaccctcc catcatcaaa tctgtaatta tttttacgtc atcttctttt
    49681 aatataagca ggttagaccc ttccaggcct tctttatact gaacctgcac cgcaacatac
    49741 ggaattggat attgtattga aagctcttcc catgtaacaa ccgacacttt tggcgtggtt
    49801 atgcttactt tgttgttcag aagagtaaat aatgtcgtag ctgaagtccc catgctgata
    49861 tttcctatct cacccagagc atctatttct tccgttgtaa gaacactatc atgaccggct
    49921 tgagaagatg aattatcttg aggcgctgtt gcgctatctc ccttaagcag ggcgtctatc
    49981 tcttcctgtg acagcatctc actcatcata atcatctcct ttcttttcta ttcgcgtaat
    50041 tttcactgac accttgttct tcttcaatcc gggcttaccg ctgaatttct ttaaattgcc
    50101 tacatatatt ttcaggtcat catttactga tgtattaagc tgtattacat cacccgctcc
    50161 aagctccagg aagtctctta cggtaatctc gctgtccccc agtacaactc ttatgggaag
    50221 cagagtgctc tgtattttct tttcgattat ctttaaatca ctgcctgtta tttccttggt
    50281 aatgcctgag aaccaaaact tggtgcttag ctttggaatt accggttcaa taaccagatg
    50341 cggaatacaa atatgaatca ttccttcaat acttccgatt ttggtattta atgttataag
    50401 cgctactgtt tcattaggtg acagaatttg tgcaaactgc gggtttgtct ctattttctt
    50461 aagtcttggc ctcaattcta ttacattttt ccatgggtct ttaaaaaagt tgataatctg
    50521 tccgattatt ttttccagga ggctaagctc aatttcggta aaacctctca ctctttcgaa
    50581 ggcatgcccc gcacctccaa gaatcctctc aatcatggca aaggaaatcg tcggtgtcat
    50641 ttcaactatt attgatccgg taagtggggc aaattccaca atgccaagca ctgacgggtt
    50701 tggcatagag ttggtgaatt cattatatgt cagctgatca accgaaataa cttctatttg
    50761 aactaatgcc ctaagatacc ccgacagata tgtctgcaac agtcttgaat aattctcgtg
    50821 catgatttga agagttcttg tgtggtcctt tgcaaacttg ttcggtatct tgaagttata
    50881 gtccctgact tttctttctt ctttttcttc ttttatctct tgaacatcta tttctccaga
    50941 attcagtgca ctaagaagcg catctatttc attctgcgaa agaacctcgg acacaaagcc
    51001 acctccttct ctactgcact ataaactcgt caaaatacac attttttatt ttctttgaat
    51061 taataatctt ttttataatt tcaacaattt ccttttgaag cttttcctgc ccttcttttc
    51121 cttccagatc tgcttcggtc ttggacctta taagtgcatt tattctgtct ctgatttctg
    51181 actccctgta ggtaaccaat tcggcaactt ctttgtcgac ctccagtttg aatgtcaatt
    51241 tgcatatttt tttgctgtct tttacattat tcacaaaaga atcacccaat gacaacatca
    51301 tgagtttttt gtcgattttt ataggttcct cttctgctac ttttggctta ttaaaaaaca
    51361 tatatattat tatacctgat actataacca aaagtaaaag cacaatagcc gctattagga
    51421 taatatttcg tttcattccg tctccctccc aagaattatg gatttaaatc cttaatatcg
    51481 tctctgagta tcaccatgtc gactcttctg tttttttgcc ggccttcagc cgtattattg
    51541 tcatcaacgg gatgctgatc agaataaccg gatgcggaca atctgtacgc atatatccca
    51601 ttttcctcta taagatattt aaccacattt actgccctgg tagtcgaaag ctcccaattt
    51661 gacggaaatc tgaaggtatt aatcggtaca ttgtcggtat gtccttcaat tctgataatt
    51721 ttgtcgttct gcctgattat ctcagccact ttattgataa tatcactggc attgggagtt
    51781 aagtctgcct tgccggagtc gaaaagaaca ttatccataa atctcaccag cagtccccgt
    51841 tcatccaggc gaagagtaat actcgcttcc agattatttt ccttaatata ttcactcatt
    51901 tgctcataga gcttcttgaa ttcttccgct tcttccgtag gagaatcagc cggaaaggca
    51961 ttagcaaggg gttccatgct caaggtcatt cctgagtcta atacaccgag agaaccgctc
    52021 atagattgtt ttatagcttc aaatttctga acatcaagag ttgcaaatga aaaaagtaat
    52081 acaaaaaaac acaaaagaag ggttaccata tctccgtaag tagacatcca cgcaggtgct
    52141 ccgggacttg gctcatcttg aactcttttt ctggccattc ttattcctct ccctcttttg
    52201 gttcctttgc tgaatcaagc atgttcctaa gttttggcga cataaaggct ttaagcttct
    52261 cttcaattat tctcggattc tcgcctgcct gtatcgacag cattccctcc agcattacct
    52321 ctttcataag agattcctga gaactgcgta tcttaagctt gcttattaca ggattacaga
    52381 atacgtttgc aataattgaa ccatataatg tagttacaat tgccactgcc atgttgggac
    52441 caactgttga aggatccgac aggtttgcaa gcatgtttat caagccgata agtgttccca
    52501 gcattccaaa acccgggcct atagcagcca taaactcata tacaccttga ttgctcttat
    52561 gtctttcttc aataaaagac agctctgttt caagaatgtt tcttacaagt tcagggtcgg
    52621 taccgtctac aatcagcagt atccccttct gcagaaagtc atctttaagc tgctgtgcag
    52681 cctcctccaa agcaagcagt ccttcctttc gcgcagtatt tgcaaggtca attattgatt
    52741 ttattatata ctcagcgtct gtctccgcat atacaaatac atactttaaa gcggcaatac
    52801 cttctttaag cttcttaatc ggaaatgcca taaatgtcat agcaattgtt ccgccaaaag
    52861 tacagattat tgaaggtata tccacatatc caatcagatc accgctgatg agtatggtta
    52921 taagaataac agctattccg cctaccagac caaatactga agctaaatcc aattatttgc
    52981 acctcacttt agaagttatc tttaataatt agcgatgaaa ggtcaaaaat cctcttttta
    53041 tattcgataa ttttttcaat aatttcctcc ggactttcag aaaccaacaa ttttttacca
    53101 tctcttaaag taataacagt atcaggagtg ttttctaaag tttcaatgtg ttcgcaattc
    53161 agataatata caattccgtt gagtcttgta acctttatca taaaactgcc tcctttcatc
    53221 attttacaaa tccccgggca ctaggcccag ggatttgcat aaacattatc tcttcaagtt
    53281 aacaagttcc tgcagcattt catccgaggt tgttattatt cttgaattag cctggaagcc
    53341 tctttgagtg ctgatcatct cggcaaattc gttggatata tctacattcg acatttccaa
    53401 ggtgcccgag ttaagtgatc caagtccgct gaagccggga agcccaataa tcggatcccc
    53461 tgagttgggt gttacttgga acatgttctc cgctgtcttc tcaaggcctg ccgggttctt
    53521 gaatgctgcc agtgctatct gcccgattat cttgctcatg ccgtttgtga atataccgtt
    53581 gatctcccca gtaggcccta tgctgaaagt gtctaaggaa ccttgggggt atccatcaga
    53641 acctgtcatc gctgcattgg attcattggc aaactgggtt agttttgtaa agtctatttt
    53701 caaatcttca atatcacctg agccattctt acctggaact gtaagctcaa caattccatt
    53761 agtgcttgtg gtatctaacg ttccatcagg cttaaaggta agtccatgag taatagtctc
    53821 taaagtgtct gaagcagcaa atccaccact ctcattatag tatttgccta agtcctgatc
    53881 cttgataaat acctgccatt gcgaagggcc tccgcctact gcaccatctg cttccatgcc
    53941 tctgataaat gtaaatttaa gtgtatgttt ccctccaagt gtatcgtata ctgtcgaggt
    54001 tgtctcccag tattttgtat ctgctggaat tgtcaaggta tctggggtat tctcatctcc
    54061 acccgctgtg aaaacgtaat ccgcatctgc tcctggtgcg actgcaatct gattgtcaag
    54121 attaccctca aaggttgcat atgtagtttc ctttgctgct accatctcgg actttgatat
    54181 ctttacccct tccaactggc tcctcaaaac atttgtatcc gggtctacca tataccctaa
    54241 tactttaaat ccattggcag aaaccagatt gccgtcgtca tccagtgaga agtttccggc
    54301 tcttgtatag gatctgctca atccgtcttc cgaatttgaa agtataaaaa atccgtctcc
    54361 gtttattgcc aggtctgtca ggttatccgt cctctgcacc gcacctctta catgaaaggt
    54421 gtcaattgag gaaagtgata ttccaaggcc tacctgctgc ggatttgtgc ctcccttgcc
    54481 tgcttgcggg gagcctgccc ctcttaatgt ctggttgaaa acctcctgga aggttgccct
    54541 ctgtgatttg tatccgactg tatttacatt tgctatgttg ttgccgatcg tatccatctt
    54601 gagctggtgt gccctgagtc ccgatacgcc ggaaaacatg gatctcatca taataaatga
    54661 cctccttaat tattagtacg cctttcttct gtcattccga aaggctaagc ctccttaaaa
    54721 aggtccggct tatattatta ctgcaccgtc gatatttgtg aacacgttgt tcttcagatt
    54781 gtcttccgta gcagctgtga ttatcatttt attctttaca tttgctatga aagctgaatt
    54841 gcccattatt atcagtgttt cctttacacc cttctcagcc gctttgttca ctgcttcgtt
    54901 cagtttgttc aaatcatctt ttgtaagatg tatgttcctc atctgcagtc tttctgccgc
    54961 atgcttcgag aatttcactt ccgattgtct cgataactcc tgctgcaata cagcgtcgaa
    55021 gctaatgctc ttatcagatt gtctgccatc tgaaggtttt gtcggctgta taggtttccc
    55081 aatgggaatt atcggtttgt tgttataaat tacactcatt cgtatcacct cttccaaggc
    55141 ttagtttact tctgtaatat cctccaaata tatctctttt ccgtttactg tcaggtatat
    55201 tgatgttcct tcctttcgta ccttctcaac agttccttca atttccactg tcttgattct
    55261 ctcaacaccg tttacttttt cagtctcttc atatgtgccc ttgacattct ttcctatcag
    55321 attgaaggct gtggtacttc tcatggtctg attcataagg tatatctgct ctacagaatt
    55381 ctcaagctca gtaacttccc tgacatcgtc aaacttgaca tcgtatttcg tacccagcac
    55441 gtccattgta agatatattt cgttgtcctt taacataacc ttttccacca ggcctatcaa
    55501 ctcttttgtc tctgtggaat cttcagctct gtagttagcc ttaatcagct tgttaaccat
    55561 attgttggct gaattcatcc ttattgcctt gttggaattc tgtgattgct ccaatgagct
    55621 gaactgtgcc atctgtccta tgaactcatt atccttggtt ggttcaaggg ggttctggta
    55681 tcttagctgt gttaccagta ttttcagaaa cgcatccttg tcaaggttac tgttgtattc
    55741 cttcggtttg ttcggttcat atgcagtgta ctggccgttt ataccgctta ccggcatttt
    55801 ttcacctcct atgctctttg atcaaatctc cctgtgtgga tactgtatgg gttgattgcc
    55861 gctgttgttc cgatttcatc ctgatatccg ccatacgtgt catgcctcat attttgggcc
    55921 cctgacttaa gcttgtccct ccatgtctca taattcttac tgaagttaaa ttccttgctt
    55981 tcatggccga cagaaacact taagttctgt atgtccagtc ccttatcctg aagcatgtcc
    56041 ctaagctcat taaagttcga ctcaattact tgcttaacct cgtagctttc cgctgtaaac
    56101 tttgctgtaa tcacgcctct ttccacagca attcttaatg taagctttcc aagattttca
    56161 ggttccagct gcattttcat ctctgactgt gtaccggtaa gtattacttc cgcctttttt
    56221 actatttggt tgataatctc aaccttgctt accggttgtg cctttggcat ttcagcctga
    56281 ttctgaactg tctcggcttt gctgaaaata atattttgtt cctgcctgac agcagttccc
    56341 tccatatcca acttattatg ggttgtatta atcgtatcat gggtttcttt tgcagcgtcc
    56401 ttgcggttat tgtttttacc cgtttcctga accttctccg gattttcttc tacagacacc
    56461 ttctcaacct tatcatccat agctgctttt atgttttctt cctttgcgtc tgtctccgcc
    56521 ttgtcgcctt tttgttccgg aactgaaggt ttgcttgagg acactgtttt tacatcattc
    56581 ttttggcttt catcaggggc cggcatcttg ctgctctcat ccccaaggtt atcctttgca
    56641 tccgcttttt ctggcgtagg tactttaacc ggattttttt ctgaatttaa ttcttctgtc
    56701 ggaatgatgt cattttgtac ctcagccgat actgcagctt tcgttccggc aggcttttca
    56761 aatattgcta ttattacatc ccgtaacttt gaaggaacat tctcaagctc tgacaccatt
    56821 tcacagatac ctgcagcttc ctctccggaa atttcggttc ggatttccgc cagttcctgc
    56881 agcagttctt taataccttc aagaagtgcc tgcttttctg cgtctgtgtt ttctccatcc
    56941 ttcattttat tcaatttttc aagcttcatc agcagttcct tgacaattgc ttccttaaca
    57001 gcttcatcat cagcaactgc cttttcaaca tttgcaccct cggcaattac atcattggca
    57061 atcacatcct tgaatcttac ttctttttct gctgccggat tttcatcccc cacatttaca
    57121 ggttccggat ccctgttgct gcttttctta tgtcttattt ccgccctgcc ggcaagttcc
    57181 atttttgtat cttcttctgc tgctgttttg gtatcatatc tgtctcctga ttttaatgta
    57241 ttcctaaaaa tctctctgaa gctgctgaac tcattttcag gccggccttg gaaaaatgct
    57301 gcattgctgc tgttatccgg tttgctgaca ttaaatgcgg gcattggtcc aatgctgaac
    57361 gtcactatct cacctccttt caatctgcaa gatcctcaag aatctgtgct gcctgcagcg
    57421 gatccatctg cgcaagaata gaggcagctt tttctttttt catgtttctg agaatctgta
    57481 ctgcagaagc cttgtcattc atattcaata ttatctgcat ggctttttgg ttatctactt
    57541 tttccaggta ctgtacctgc tcttgaatgc tccggagctt ttcgctcaat tgctcatgta
    57601 atttgcttat ttccaattcc ttatccgcta attctttttc ttttttttcg atttcctttt
    57661 cgcggcttaa aagctctttc tccagattat tcaattcttt ctgctgtatc gcaatgtttg
    57721 acttttccct ttcaattacc tctctttcaa tctgctcagg attctttttt tgtaacaccg
    57781 gctgcaacag tttgcctaca accggcagat tgcttatcca cacagaagtt tccgccctga
    57841 gccctgcaaa attaaacatg aatatagctc caaaagccac tatggcggac aaaaatatta
    57901 ctataatcag tagaagcagt gtatttgcct tcttttctct agttttaccg cgctttgaat
    57961 cagccttttt acttttttct gctgcgggtt ttttttcatt ggtgtttgat ggttgattca
    58021 ttagcctttc ctcctaagat ttgttgcttt ctttaaatga aaccaggtcg tctaccagct
    58081 tttcttcctt tttttgttct tcatataaat acttgctgta atccatttct ttaagctttt
    58141 ctatcatcct tttttcccgg gtcgctgcta taagctcttc cctgcaggca gaaagataat
    58201 cctcagccat tttaatcctg actttttgtg cttttattcc cctcttataa taatcgatat
    58261 aattagccaa tatctttagc tcatttactg ccattccttg agaagttttc tcctgaaaca
    58321 agctttgcat atcactgaat tcctttttca actccgccat cttgtgcttt tctttttcaa
    58381 gcctctttac agcggagccc agcttgtttt ttttctcttc ttcgactttt gttttgtaat
    58441 ccaaaagctt ttgcagtctg aacttatata cagccatacg cttctcctac gacactataa
    58501 ttctcatttc gttcaaaatc tcttccattt ctgtcttttc atcaacgcct tgcttgagga
    58561 atctttcaat tgctcctatt ttttctattg acctgtctat actgctgctg cttcctttta
    58621 catatgcacc tatgtttatc aggtcttccg cgtctttata taccgacatg atgttcttga
    58681 attcatttgc attttcctta tgctcatcat caacaatatt gggcattacc ctgctgacac
    58741 ttgccagtac ctcgattgct gggtagtgat ttttatttgc caggcttctt gacagaacta
    58801 tatgcccatc caatatgctt cttactgtat cggtaattgg ttcgttcatg tcatctccgt
    58861 caacaagaac cgtatacagt ccggtaatgc tgcctttatc cgatgttccg gatctttcca
    58921 gcagtctcgg catcacggag aacaccgaag gagtgtagcc tcttgtaaca ggcggttcac
    58981 ctattgcaag ccccacttct ctttgagcca tggcaaaacg tgttaatgaa tccatcatta
    59041 acatcacatc cttgccctga tctctgaaat actcagctac ggaagtagcc agctgtgcag
    59101 ctttcagcct tattagtgca ggctggtctg atgttgcaac aaccactaca gaacgtgaca
    59161 gcccatcctc tttaaggtcg ttttcaatga attccctgac ttccctgcct cgttctccta
    59221 taaggcctat aacatttata tccgcctttg tattcctggc taccattccc attaatgtac
    59281 tcttgccgac tccgctgcct gaaaaaatcc cgattctctg cccttttccc aatgtaagcg
    59341 gcccgtcaat agctctcact cctacaggta atatctcagt aattcttttt cttgaaagag
    59401 gattaggagg ggagttttct acaggataca tagtgcagtt gttaatagtg ccctttccgt
    59461 cgattactct tcccaaccca tcaattactc tgccgagcat atcattggat actcccaccg
    59521 agaggctttc tcccgtagct tcaattttgc ttccggggcc tacgcctttc atgctgccca
    59581 aaggcatcag gcacactatg ctttccttaa aaccgacaac ctccgctttt attggggtgt
    59641 ttcccttcaa agggtatatg ttgcaaatct ccccaaggtt gactgcgggc ccgtgagatt
    59701 cgatggtcaa accgataacc tgggaaaccc gccccgtgta ctttataagt tcgttgctta
    59761 acaaagcctt attatacttt tccatgtcaa tgcccatcat tgctgatcac tcatttctca
    59821 acaaatatgt aaatattttt tttatttcat taaattgaac ttcaatactt gaattaatct
    59881 caccggaagg tgtttcaaca atgcagcttc ctttcggaag tgataaatcg gaaactatat
    59941 caatatcgga tatgccctct accatcttgc atatccgata tttgttttct ttaacgaatt
    60001 caaaatcaga aggtgatatt ttcaagacaa gcttgttttt aaatgtgcat ttttgcagcg
    60061 cttgattaac cagggacaga agcgtttctt tattctgtga taattcctgg cctaatactt
    60121 tctttgagat actcagaaca agatccacta tctgttcttc cgcctctttg taaaggctct
    60181 ctcttcttct gtcaaggaat tcttttattt ctgaagcttc atcaattatc gactgtgctt
    60241 cgttttttcc ttcaagatat ccggattcat aaccttcatt gtacccctct tcccttgaat
    60301 tagataagag gctccgagct tcctcttcag ctgcttccat tattctgcgg gcttccgaat
    60361 ttgcttcttc tattatcttc aaatacattt gcttggcatc ttcaactatt gaattggcat
    60421 attcttcttc atcctcagta ttgttatcat cggtgtttat tgttttgcac tcttcctctt
    60481 taattgtttc tttcctgaag acatgaacta aatgtttagg aaatcctata gttatatttc
    60541 ttgatttgta aaccttagac aattatttca tctcctccgc ccctagatat aacaatctcg
    60601 ccggcatcct ccagcttcct gataatattc actatcttct gctgtgcttc ttcaacatct
    60661 cttagtctta cagggcccat gtattccata tcttctgtta tcatctcggc aagacgtttt
    60721 gacatattac tgaaaattgc ttttttaact tcatctcctg cattcttaag agccaatgcc
    60781 caatctgaat tttcaacctc ccttgtaacc ctctgtatgg atctgttgtc aagagttaca
    60841 atatcgtcaa atacgaacat tctcttcctg atttgctcag caagatcaat atcctgtact
    60901 tccagggtct ccattatgaa tttctcagta ccgcggtcaa ctgaattcag tatatctaca
    60961 accgcagcca aacctcccgc gattgtgtaa tcctgtgtaa ccatagatga aagcttcctc
    61021 tcaagaactc tttcaatctc ttttattact tcaggggatg tcctgtccat aattgccact
    61081 ctgcgggcaa catcagcctg cttgtcctgc ggtaaggatg aaattatggc agccgcctgc
    61141 tgaggcttca gatatgccaa tattagtgca attgtctgcg ggtgttctcc ttgtatgaag
    61201 ttaagaatct gagccggatc ggcttttctg acaaagtcaa agggtcttac ttgaagtgag
    61261 gcagtaagcc tgtttattac gtccaatgcc ttttgtcccc caagggctct ttcaagtata
    61321 tccttggcat aagaaatacc accctcggaa atatactcct gggcaataca tatctgatag
    61381 aactcattca gtacctcttc cttctcctcg ggagttactg ttctcatatt ggcaatttcc
    61441 aaggtaagct gttcaatatc ttcttctttt aaatgcttga atatagctgc agacttttca
    61501 ggtccgagtg ttatcagaag aactgccgct ttctgcctgc cgttaaaagt tccttttaat
    61561 cctcttgaca ttacataacc tcctactcct cattaaccca ggtttttaac agttgtgcga
    61621 cagtctcagg cttaagcccc acaaactttt ctatcttttt cttaacttca tttttatcct
    61681 caatatcaat ttcagcaata ggttcacctg ataatgcagc ctcactcatt gcccttccgg
    61741 tgctgtccac agcatatcct gatccggcac cataggctcc ggtccgtttt cttaacatta
    61801 taaatatcac tcctccaaag acaagcaatg cggcaacacc tgttccgatc ataatgatac
    61861 gttcccagaa aactgctttc ctctctgctt cctgatcagc tttaatttta ttggctaatt
    61921 ccatatcaaa gggcgcaaaa actacttcta taccctcatt ttcttctgtg gatgccgtat
    61981 aattaaaacc ttgcaaagcg tattttacta atgctccgac cctatcctgc aattcttgcg
    62041 ggatttgctt cggattccct tcagaatcca atgtatttat aagcaccgat actgataagg
    62101 actcgatact tcccatttct tttataatct gttcttttat ttcatttatt tcatagttta
    62161 caattgtact tgcttcattg tactgtgctt tggatgaatc cacctctgcg tattggttta
    62221 tgtcggtatt tgaatcggtg cccggtacgc ctccctgacc tgcgtcaacc cattcttttc
    62281 ttatatcctg catatttctg acgatgcctt cgttttgttc cgtattagga gcttcatagg
    62341 ttgttatgtt ctgtgtaaca ctgtcgtaat tcagctttac tgctgctttt accttgacat
    62401 tgtttattcc gaagggagct tccaggaatt ttgtaatgct tttttcaatt ttgtcctgca
    62461 cggcttcctg catttccatc tgggatgaaa taattgaatt aggtgttcct gtatcaggca
    62521 caagggacct tccgttttca tcgattattg agatattttc aggttttaaa cctttaacgc
    62581 tccttgcaat atactgaacg ataccgttta cctgatttct gtcaagagcc ggatatcctg
    62641 gcttcatttt gattataagg gatgcagttg cttcactgct gttttgatca attataaaag
    62701 tctgatcctc cggtatatac agattcacat ccgcatactc tacaccttct atagcgctca
    62761 gacccttttt aagcttgttt tgcttggcta taagatatct ttggttcttg tcctggcttg
    62821 tctcataaaa agtggcctgc agttcctcag gatacaatat atcatcacca aagattccct
    62881 caacagccaa ttgagccctc agctcatcta tcctcttaac cggaacgtaa attgtagtgc
    62941 cttcaattct tgtattcaca ttcatttctt tcagtcttgc aataattgtt cctgcatcac
    63001 cctggctaag atccgaataa agtatcccca tctgaggccg gctcatatat gttataaaac
    63061 tgacaattaa aacgaaagac aataatccgg caattattat cctcactttt tgtccttttt
    63121 tcaatccgtt ccagaattct acggtattgt tccttaaatt attaaaaaat tcacccattc
    63181 catcacctca tgaaaccgca aaaattttta tacaggcatc ctcattattt cctggtatgc
    63241 ttccaaaacc ttattccgta tttgaacggt gaattgtaaa gcaatatccg ccttttccga
    63301 agctatcaca atctgatgca gattatcatt caagcccatt gcaaaagcct gattcaattg
    63361 ttccgattct gtctgcagct tgtctgcatc ctttattgca ttattcaaaa aatcggtaaa
    63421 agataatgct tccttcctgg atgcggcatt attattcaaa tttgaatcaa tgccaaactg
    63481 tccgacagga aaattaattt tcataataat atcattcctt ccctaaaatg gctaacattt
    63541 ttatcttctt ccaatttcca gtgcctttag tgccatactc tttgtggagt taattgctgt
    63601 tacattggct tcatatgccc tgcttgcaga tatcatattt accatttctg tcattatatc
    63661 cacgtttggc atctttacat agccgttttc atcagcatcc ggatggcccg gatcataaac
    63721 cgacttgaag ggagtcttat cttctttaat accggtaacc tttacaccgg caccaataag
    63781 cctcctgctg gcatcactca ggtattcact gaagggcgta ctgccttccc cttcccggaa
    63841 aaccacaacc tgccttctgt aaggagttcc ttccgcggtc cttgtagtat tcacatttgc
    63901 aatatttctt gatattacat ccattctcag cttttctgca gttaatccgg aagcactaat
    63961 attgattgaa ttcaaaaatg ccattgtaat tacctcccgt taatcgcaaa ttttatttta
    64021 gcgaattcct ttgacaactg agctattaaa gcattatatt taattgtatt cttagcaagc
    64081 tccgccatct ccacatctat atcaacgtta tttccgtctc ttctcataga agtggagttc
    64141 tcccgtacaa tttcaggatt ggccgatata actctatcct tgcctatcgg caaaaatctc
    64201 ggatctttct tggcgccgga tatctggaac ttttcctgag cgtcagccat atactcctcg
    64261 aatcttacat aggatttttt gaatccggga gtattaacat tcgcaatgtt gtttgaaatt
    64321 gcttcatttc tcatccaggc cgcatcaaga gccttttcaa gcattaaggt cttattgctt
    64381 atcttatcaa tcataacagg aacctccata aaactccata atagaatttt aattgccaga
    64441 aatacctata tcagttagta tttcgacata aattcaacat ttcctccgtt ttcaaggaat
    64501 aaaaatatat tatttacata attttaactt ccatatatat tatcgaaaaa aaagcaaatt
    64561 cctttcatcc tccgctaaaa aatttcgcaa aaaaagaaga atgcgacggg gacacgggga
    64621 aggggacatg gggacgtttc tcctgtcccg ctgaaagcgg gacaggagaa acgtccccat
    64681 gtccccaaat agcattccac taatccttaa tgatgattct accgtacttt tttcagttct
    64741 tccagcagct tatcattcaa aatccttata tgtgtcccct tcatccccag ggatcttgat
    64801 tctattacac cggcactctc aaattttctg agtgcattta ctattaccga ccttgttata
    64861 cctactttat cggcaatctt gctggcaatg agcaagccct catttccatc cagctccttg
    64921 aatatgtgtt ctatggcttc caattccgaa taagaaagag ttccgattgc catctgtact
    64981 actgcttttt ttcttgcttc ttcttcaatt tcatcatgtt tggatcttaa tatctccagg
    65041 cctactaccg ttgctccgta ttccgccaaa acaaggtctt cgtcagtaaa ttcctggtta
    65101 tactttgcga gcatcattgt accaagcctt tcaccgctgc cgttaacagg aacgatagta
    65161 gaataatttg tacccatatc gcacctttct ctgttgtcaa taacgcacag tttatcttta
    65221 tttatcaggt ttgatcttgt ttcatggtat gagagcagtg atttattgta ttcatcagga
    65281 aatttcttct ctgtcagcag ttcgttcttt aaaaattcgc actcctctga ggtgcccaat
    65341 gagtatccca gtattcttcc ttttctgctc aatatatata catttgcgtt taatacctca
    65401 cttagtattt tgcttagatc atgaaaggat acggattgag tgcccgatgt ctggagtacc
    65461 ttgttgactt tcctcatctt ttctaatagt gttgaactca tatttatcct ccttattaaa
    65521 taatataatt gctgacatct tttctcttaa tatcgtgaat caatgccttt tgcacatatt
    65581 ctttcgtaat attcaattca ccgctcttca tatcaggcgc ttcatatgaa atatcctcaa
    65641 aaagtctttc cattaccgta tagagtctcc ttgctcctat attctcgtac tgttcattca
    65701 taaaagctgc aatagatgca atctcttcaa cggcatcctc ctcaaagttg atatttattc
    65761 cttccgtacg cagaagagcc ttgtattgct ttataagagc attgttgggt ttggttagta
    65821 tctcaacaaa gttgttcttg ctcaggctgt ctaattcaac ccttacaggg aaccttccct
    65881 gcaattccgg tatcaaatcg gatactttgg ccacatgaaa agctcctgcc gcaataaaca
    65941 gcatgtaatc cgtctttaca gggccgtatt ttgttataac ggtacttcct tcaactatgg
    66001 gaagtatatc tctctgtaca ccttcccttg acacgtcagg gccgccgata ttgcctctcc
    66061 cggcaatttt atcaatttca tctatgaaaa taattcctct ctcttcggct tttttaacgg
    66121 cttcttctat tacttcatcc atgtcaatga gcttttgtgc ttcctgctgc gtcagcagct
    66181 tcaacgcttc ctttactgtt actcttcttc gcttagtctt tttgggaagt atgcctccga
    66241 acatatcctg aagatttatg ctcatttgat ccatccccgg ccccgataac atttcaatgc
    66301 tgtgcccgga tgtatcttct atctcaattt ctatattctc gttgttcaaa tccccgttct
    66361 taacattttg cattacatag tatcgattgg cagccaaact ttcttccttt ttcttcgttt
    66421 cctcatcact ttcggtttct ttgcttccgc cgtaattaaa tatcatttca aagggattct
    66481 tggacatgct gcttttgttt gaagaaggaa caagtatctc ggcaaccttt tccgccgcta
    66541 tatcaaatgc cttttcatta acctttgata tcttttccgc ctttaccaac cttacagaaa
    66601 attctatgag atcccttatt attgattcaa catctctgcc tacataaccc acctctgtga
    66661 attttgtagc ttccactttt acaaaagggg cattaaccag cttggctatc ctgcgtgcaa
    66721 tttctgtttt tccaactccg gttggcccta tcatcaatat attcttggga gtaacctccg
    66781 cccgcatttc ttcatccaga agatttctcc tgtatctgtc cctcaaagcc acagctactg
    66841 atttttttgc cttatcctgt ccgatgatat atttatccaa ctctgctact atttgtttcg
    66901 gagttaactc gttcatagat tcctcctata attcctctac aatgatgtta ttattagtaa
    66961 atacacaaat tgaggcagct atttccaagg acttccttac aacctcatgg gacgggaggt
    67021 ctgtgttctg aatcagagct ttggctgccg ccagagcata attgcctccg gaaccaattg
    67081 caattacacc atcatccggc tctattacct ctccgcttcc agaaataaca agcagagttt
    67141 ctttatccat tgcaatcagc atagcctcca attgccttag tactttatct cttctccaat
    67201 cctttgccag ctcaactgcg gctcttttca gattgccgct gtgctgctcc agcttttctt
    67261 ccagcttttc ggaaagggta aaagcatctg ataccgagcc ggcaaaccca accagtactt
    67321 tcccgtcaaa taatgttctc acttttttgg cagttgactt gattatggtg ctttggctga
    67381 aggtcacctg accgtcaccg gcaatcgcac ccttatcgtt tcttttgact gcagctattg
    67441 ttgtaccatg aaacatagtt ctcctccagt aatctatttc aaatatgtta caatattcat
    67501 aaaagtcaaa aaactcgcta gattcaatat agcatatgaa tatatatatt tcaaccactt
    67561 agagccgttc acgaataaat ttttccaagt cctttaaagc tctttcagca taataactgc
    67621 ttttttcctt tttgtttttt attctgtatt caggctgcgg gaatattcca aaattcacat
    67681 tcatcggctg gaatgccttt attgtacggt ccgtgatgta tctgcacaat gctccgtggg
    67741 ctgtagtttc aggaaataca acgggttccc tcccatctat gatcctggcc atatttattc
    67801 ctgcaaccaa accggaagaa gcggactcaa cgtatccttc cactcctgtc atctgccccg
    67861 caaaaaacaa cttgtcgttg ttatggaatt gaagtgtggg cctcattacc ttcggtgaat
    67921 ttatgaaggt atttctgtgc attacaccat accttacaaa ctctgcattt tcgagtcccg
    67981 gtatcatgcc gaacactctt ttctgctcac cccatttaag gtgtgtctga aaacctacaa
    68041 tattatataa agtaccctcc ttattatcct gccttagctg tacaactgca taaggctgtt
    68101 ttccgctgcg gggatcggtt aagcccacag gctttaaagg cccgaacagc aatgtctgca
    68161 atcctctttt cgccatagtc tcaacaggca tacagccttc aaaaacaacc tctttttcaa
    68221 agctcttaag tggtgcagtt tcagcattta caagttccct gtgaaaattc acgtattctt
    68281 cctctgacat gggacaattt atataatcac tgtcaccttt ttcatacctt gatgccatga
    68341 ataccttgtc cttgtttatt gattcatagg ttataatagg agctgctgcg tcataaaaat
    68401 ataaatagtc gctgtccatc atcctgccta tgctgaaaga aagcttttcc gaggttagag
    68461 gccccgttgc aactatcaca tattcatttt caggaatgca tgttacttct tcccgtataa
    68521 tctctacatt ggagttaccg gtaattctgt cagttatttc ctgggaaaaa ccctctctgt
    68581 ctacagctaa cgcaccgcct gcaggcaatg ctttgctgtc ggcacattcc attatcagag
    68641 agcctaatct tcgcatctcc tctttcagca gtcccacggc attctccaac tggtttgagc
    68701 gaagggaatt gctgcaaacc aattccccga acttatcggt atggtgtgca ggagtactgg
    68761 ccacaggcct catttcataa agccttacct ttattcctct tttggcaagc tgccatgctg
    68821 cttcgcttcc tgccaaacct gcacctataa ctacggcctt ttccttcaag actatttcac
    68881 ctcgttatta tctgaccttt tgtaaccgca tgcttcattg atgcatttgt attcaaaacc
    68941 ctttttgtta tacttcttca caataaggct tccgcactca gggcatttct cggcaacagg
    69001 ttcgtcccag attacaaagc tgcattccgg atatttgctg cagccataaa acttccttcc
    69061 ttttttggtt cttctagcaa ttaattcccc cgtacaattc ggacagttta tgcctgttga
    69121 ctcaactatg gcctttgtgt ttctgcagtt tggaaaaccg ggacatgcga ggaatttgcc
    69181 gaatcttccc tgcttatata ccatgtttct gccgcagaat tcacagacga catcactgac
    69241 ttcatcctgt atctttattt tcccgacctt ttcttccgca cttttcagtg ttttttcaaa
    69301 gggcccgtaa aactctctta ttacatgcac ccaatccttc tcgccctctt ctatattgtc
    69361 aagcttccct tccatattgg cagtaaactc ggcatctaca atatcagaaa aatattcctt
    69421 cataatcccc gtaacctttt cgcccaaatc ggtaggaaca agaaatttct tgtctcttat
    69481 tacatagcct cttgccagta tggttgatat tataggagca tatgtgcttg gcctgcctat
    69541 tcctttttcc tcaagaatct ttacaagggt agcttccgta tatcttaaag gaggctctgt
    69601 aaaatgctgc ttgtgctcca gtttcttaag cttcagcaac tgcccctcgg taagtctcgg
    69661 cagtgatatt tcttcttctt ccttttcatc gctgctttct ttataaacac tcatgaagcc
    69721 cggaaacttc aaaatacttc cgtatgcttt aaaaatatag ccgtttgctt caatgtcagc
    69781 cgtgacggta tcgaatacgg cagcctgcat ctggcttgca acataccttt cccaaataag
    69841 cttgtataat ttgtattgtt ctttatcaag ggagtctttt atgctttccg gttctctgta
    69901 aaccgaagta ggcctgatgg cctcgtgggc atcctgtacc ttcttcccgg acttgttctg
    69961 cttaatctcc cccccaagga aactctttcc gtatttattc ataatatatc cggcagcatc
    70021 ttttttggct tcctcggata ttcttaccga atcagtcctt atgtaagtaa caaggcccaa
    70081 ggttccttcg cccttgatat ccacaccctc ataaagctgc tgcgctacca tcattgtcct
    70141 cttggcagta aacccaagct ttctataggc ttcctgctga agagtgcttg taatgaacgg
    70201 gtttggagag tttctccttt tctctcccgt tttgattctc ttgacagtat attttgcacc
    70261 cttaagagcc tcaagtattt tttccatttc gctctcgctt gagatctcaa ttttctcatt
    70321 ttctgtgccg aagaattttg catcaaagct ctttcttgta tcgggatcat tcaactttgc
    70381 tgccaaagtc cagtattccc tgggaacaaa tgttctgatt tcatcttctc tgtcacaaat
    70441 aagccttact gccacagact ggactcttcc ggcacttaag ccctttttga ccttcttcca
    70501 gagcagcggg cttattttgt atcccacaag tctgtccaat actcttctcg cctgctgggc
    70561 atctattaaa ttgaaattaa gcttccgcgg atttttcaag gcttttgtca ccgctgactt
    70621 tgttatttca ttaaactcaa tccgtgatac ttccctggtg ttcagattaa gtatatttgc
    70681 cagatgccat gatatggctt ccccttctct gtcagggtca gtagcaagat ataccttgtc
    70741 acagtttttt gcctctttgc gcagcttgtc ggtaatttcc cccttgcctc ttatagttat
    70801 gtactttggc tcaaagttgt tttctatatc aatacccaat tgacttttcg gcaagtccct
    70861 tatatgtccc attgatgcct caaccttgta gcttgaactc aggaactttt ttatcgtttt
    70921 tacttttgca ggtgattcga ctattatcaa tgatgttttc aaataattca cctcatttta
    70981 aaaattcaaa tcatattata acagaaaatt agaaaaaatc aaactaaatg gtaattcccg
    71041 cctgtttgcc gaatcagccc tttaacctcc agcattgtca atgcagccat cgccttaccg
    71101 gtttgaagcc ctgatgccgc tgcaatttca tccctgtcgc cgccgcatga atgaagtaat
    71161 tccagtatca gcctttcctc agcgcttatt tcgcagcttc tattgtcaaa cgtattgctt
    71221 ttttccctgc atataggttc aatattaaat tcctccagaa tatcttcgat tctgcttaca
    71281 agctttgcgc cgttctttat cagttcattt gttcctttgc tgttggtaga ggttatgttc
    71341 cccgggacgg caaacacctc cctgccttgt tcaagggcaa aatcggcagt aataagggat
    71401 ccgctctttt caccggcctc aacgacaatg acccccaaag acataccgct tattatcctg
    71461 ttccttgcag gaaactgaga aggatatgcc tccgtatcaa aggggtattc tgatatcagg
    71521 gcaccccttt tctcaatttc cttgctcaat ctgaaattag ccgcagggta aacatacttc
    71581 agcccgcatc cgaatactgc aattgtcctt ccgtttgccg ctattgctcc cctgtgtgct
    71641 gcggaatcaa tccccatggc cattccgctt actattgtga tacccgactc tgccagacgc
    71701 aaagctatcc tatgagcggt cttcagacca taatcgcttg ctttccgtga gcctacaata
    71761 cttattgcaa ttgaatcccc ggctgtcagt tcacccttta catacaatac ggggggcgga
    71821 tcatatatgt gcttcagatt ctcgggatat tcattttcaa atatagtata gacttttata
    71881 tcattatctt tcacaattct aaaatattca tttacttttt ctgtatttct gtccgcttta
    71941 attgattctg caatcttttc ccttacaatt ccagagttca taagctctga aaatgagcaa
    72001 tgaaatacgt tttcagccga gctgaaatgc tttacaagcc tcaggcaggt tttgcttccg
    72061 actccaggaa tagaagacag ccacataagg tattttttat ctttcatagc aacccccttt
    72121 gaattgatac aattttgtta caatcttcat taaattgtta cactatgata acaactttgt
    72181 aatattgttc tgatataata aaatcatcaa ggatataact ggaattaatt cttccccctc
    72241 ctatttttta accggtagtg tgatgctacc ggtttttaat tatcttttat ttttatattc
    72301 ctttatgtgg cgcattaccg agtaaccggc aaacttaccc gtgtttgtga ctatcctgac
    72361 ggttcctctg ccaatggatt ttaaagtatc cacactaatc tttatatcct catcaatctc
    72421 ttcaattgtc atacctgact ctttgagaaa tctttccctg gctttttcat ccgaagatct
    72481 aagtaccttc ctcagacagc gcagcattac gataagattg tccagctgtc cggcaacagg
    72541 tatgataccg ggtataagct ctatagggga gacgctgtaa gctattcccg cagaaagtaa
    72601 aagcttttgc ccttttctta tatcagcgct tttataaagt tttatcagca atttgccgta
    72661 aaccgggagt ttttctatta ccgagcatat atcacgttca ttagtgctca tcgcttacac
    72721 ctcgcatgag ttgttcataa tatgtacttc ctgtccaggc ttctgtactg aattgcctct
    72781 gcaatgtgat cgattcctat gctttcgctt tcttccaaat ccgctatggt tcttgctacc
    72841 ttcaatatcc tattatatgc tcttgcactc aagttaaacc tgtcaaatgc attcctgaga
    72901 agctcttttt cttttttccc tatggaacag tatgctgtca tatgcttggg ctttaactgc
    72961 gaattaaaaa aaattccttc gttttcgtat ctttccagtt ggagctgcct ggctctgtta
    73021 atccttttct tcatggctgc ggagctttcc tctttagggc tgcaggacag gtcattgaaa
    73081 ttcacggagg gcacctctat atgcaagtct atcctgtcca tcagcggccc cgaaattctt
    73141 cccagatagc cttttatatg attggggctg caattgcatc ttcctgattc atctccgtaa
    73201 tagccgcagg ggcatgggtt aatagagcat ataagcataa ttttcgccgg atatgttatt
    73261 gtacctccta ctcttgaaat tgtgacacat tcatcctcaa gaggctgcct taatacttcc
    73321 agcacatttt tttgaaactc aggcatttca tccagaaata gtacgccata gtgagccaga
    73381 gatacttcac cgggatgagg cactcttccc cctcctataa gcgaaccggc cgacatggta
    73441 tgatgaggag cacgaaaagg ccttcttgtt acaagatata gcttgtcttt aagcaatcct
    73501 gctatgctgt atatttttgt cacctcaagg ctttcttcaa aagacatatc cggcagtatg
    73561 gaaggcagcc ttcttgccag catagtcttt ccggagcccg gagggccgag cataagaata
    73621 ttgtgtgctc ccgctgcagc aatttccaat gctctttttg cggcattctg gcctcgcacc
    73681 tccgaaaaat catatgtttc ggcagtatcg ttccttaaaa ttgcttccaa atcaacctta
    73741 tggggtgtaa tgaacctatt tccgtttaag aactctgcca tatcagtaat gctatttatc
    73801 ggatatattt ccataccgtt gacaagggca gcttcatcgg cattatctct tgcaagaatc
    73861 agcttataga agcccttttc ttttaaggag aggcacatgg gcagtactcc ctttattcct
    73921 tttacactgc cgtttaatga caattctcct aaaaaagcaa agccttccaa tgcttcattc
    73981 gttatgtaca ttgatgctct cagtattcct gaagcaattg caaggtcaaa aactgaaccc
    74041 tcttttctta tatcggcagg ggcaaggttt actgttattc ttttttgcgg aaacagaaaa
    74101 ccggaattct ttattgcagc tcttactctt tctcttgact cccttactgc cgcatcagga
    74161 aggcccacta tgtcgaaccc cggcagcccg ttggacaagt cagtctcaac ttcaatcaca
    74221 tgcccttcaa tcccatataa agcacaggtg ttcgtcttat atatcatcat atcactccat
    74281 tttttagcat ttaatgatat ataattctat ttattcctat tatttcctct ttttatttta
    74341 aataattaca ttatttagta agtgaaaaat aatgctgcat agttataagt ttgcacaatc
    74401 aagcaagaag cttgtatagt cacgagacac gggggcgttt ctcctgtctc actgaaagcg
    74461 agacacgcga aacgtcccca tgtctcaagg ttattgctct ggaattatga ctgtaaactt
    74521 tttctcacat aaagctcata catcagtatt gacgctgcta tccccgcatt cagggattca
    74581 gccctccccg gcatcggtat cgttgtattt atatcaagca tttcttttat ttcgctgctt
    74641 attccctgcg actcattgcc tataattatt gcggatttca tttggtatgg cagctctgta
    74701 cagttaatgc tgctgtgggg gtccgcccct attgtaacaa ctcccctttg cttcagctta
    74761 tcgattattt ccgcagtatc aacgctctga ataacgggta tatggaataa agagcccatg
    74821 gttgccctta ttgtctttcc attatataag tccgcgctcc cttttgatat tgccactgca
    74881 tcaagcccgc aggcatcggc agcccttatg attgtcccca cattcccagg gtcccttaat
    74941 tcatgcagca atataaggga aagcttatcc ttcgagataa tattgtcaat atcatatttc
    75001 agcttttcag ctacagctat tattccctga ggcgtttcgg tttcacatac tctcttgaaa
    75061 accctgtcat cggcataatg cagcggtatg tttcttccgg tgagagcagc ggcatactct
    75121 tggtgtttgc tgctctccgc atagctttga ctgataagaa ccattgatat cggcacatta
    75181 taatccaggg cctcccaaaa aagcttttct ccttcaatca ggaatttgcc gtattcatcc
    75241 cttgcttttt tcatttgcag ggattttatg tactttagag tattattgga gctgcttgag
    75301 attatttctt tcatatctct ttctctgttt tcctttcaag ttttttcagg tcatcattgt
    75361 gtcctatgac aatcattatg tctccgtctt ttatttcgct gtcagctttt ggggatatat
    75421 taatactttc attgctcata tttcttatgg caacaacagt cactccgtat ctcgccctca
    75481 tgtttatatc tctcaaagct ttccctttcc agctttccgg tactgcatat tcaataatgc
    75541 tgtgttcttc cgaaagttca ataaagtcaa gtatgctgga agaaatcagg ttatgggcta
    75601 ccctctcacc catttccctt tcagggaata ctattttatc tgctccaatc ttcataagta
    75661 ctctggcatg aagttcattc tgtgcttttg caaccacatg ccttactccc agttctttca
    75721 gcattagtgt aatcaagatg ctggattgta tgtcagagcc tatagccact acagcaacat
    75781 caaaatttct cgctcccaaa gactttaata cgctttcatc cgttgcatca gcagtaactg
    75841 catgtgtaac cttatcggat attttctgaa ttgcttcctc gtcggtatct atcacaagaa
    75901 cctcatgccc cagatcgcta agtgtttcag caacagcccc tccaaatcgc cctataccga
    75961 ttactacgaa ttgtttcatt ttgccttcat tcctttctaa cctaccataa tgcggtcttc
    76021 cgggtatttg acatttcctc tgttattaat ggctcttctt gccaaagcca atacaattgt
    76081 taaaggcccg actctgccgg caaacatggt catactgatt attaattttc caaaggggct
    76141 cagattcggt gaataaccaa gagtaaggcc cacagtgcca aaggctgaag ctgcttcaaa
    76201 aaaaacagtc ataaaatccg cattctctgt tattgatagc agcattgtca caattacaat
    76261 cagactcagg cttataaaac taacagacat tgccctgtaa accaggtatt ggggaagatg
    76321 tcttccaaat acctcagtat cctccttacc tttaataaca gaaataaccg ttaaaaaaag
    76381 aatagcggcc gtggctgttt taattcctcc ggctgttgaa cccggagatc ctccaatgac
    76441 catcagaata atcgtcataa gctttgtcgg agtcgtaagc accgccatat caagggtatt
    76501 aaaaccggcc gttctcggcg ataccgattg aaagaaagaa gccagcagct tccctgataa
    76561 ggataatttt tttattgtat cgggattatt aagctccatt atgaatataa ataaagctcc
    76621 ggtcaaaatc agtattatag ttgtcgatac tactaccttg ctgtgaagtg tgacatttct
    76681 catgtccctt tcttttattg ccttgtaaat gtccatccat accgaaaacc ccagaccgcc
    76741 tattattatc agaaacatgg tatttatatt tataatacaa ctatcaacat acggggtaaa
    76801 gcttctgaag cctccgataa gatcaaagcc tgcattgcag aagctggtta cagcatgaaa
    76861 tactccaaaa gcaattcctt ttctcagccc gtaatccggt ataaacgcaa ttgtaaaaac
    76921 tattgcacct ataaattcaa tgataatagt cgcaattatt atattcttcg tcagtcgtac
    76981 taaaccttcc aggtcaaact gattcaatga ttcctgtatc agaagccgtt cctttaaggt
    77041 tattcgtctc cccagcaaaa gcgaaagcag ggtggacatt gtcataaaac caaggccgcc
    77101 tatctgtata agcaacagta ttacaacctg tccgaatgcc gtccagtaag tacctgtgtc
    77161 aacgacaaca aggccggtaa cacagactga tgaggtagct gtaaataatg aatttattaa
    77221 tccaaccggc tgtccgtttc tcgatgccat aggcatattc agaagcaggg taccaatcaa
    77281 tatcattgaa gcaaatccaa tgactattat ttgtgtcgga gtcattgtaa tctttttcct
    77341 cattatgtta aatccgatta tttcaatcac ctctgtgatg ctgctctaaa atccggtata
    77401 ctaataattt tctcaattgt gtatgcgttt ataaccgcta aaagaaaaaa gacctattcg
    77461 gcctttttct aatatgctta ttatgcgttc agcttttcct taactgtatc aacaagcttt
    77521 gtaaatgccg ctgcatcatt tattgcaagc tcggaaagca tctttctgtt gatttcaata
    77581 cctgacagct tgagtccgtt aataaacttg ctgtaggata taccgttatt tctggctgct
    77641 gcattaattc ttgcaatcca aagctttctg taatccctct tcttaagctt tcttccgata
    77701 tacgcaaatc ttaaagccct cattacagtt tcattggcta ttctgtattt tttacttctg
    77761 gctcccacgt atcctttagc cagtttaagt atctttctac gcttcttatg agcgtttatt
    77821 gcccttttta ctcttgccat gttatgctgc ctccctgtct cttatttgta tggaactaat
    77881 tttctaatca tttttgcttc cgtagcagtc gtataagcgc ctttcctcag ttttctcgta
    77941 cgctttgtag tcttcttggt aagaatatgt gacttatatg cacatgctct ttttacttta
    78001 ccggtcttgg taaagctgaa tctttttgct gatgctctgt gggtcttctg tttaggcatt
    78061 aaaaatcctc tccttctgta ttggtcatat tttgaactta tgaaatattc atagaattac
    78121 caggcttact gtttcggcgc taaaatcatt atcatatttc tgccttccaa tttggcaggt
    78181 ttttcgacca cacagtaatc cttaactata tcataaaatc ttcccagggc tttttcacca
    78241 aagcttacat gatccacttc tctgcctctg aaccttattg ttaccttgac cttgtcgcct
    78301 tcctgcagga acttttgagc attttttgct tttacattca agtcatgttc ctcaatagta
    78361 ggagagaaac gaatttcctt gatgtttact attttctgat tcctcctggc ttccttttct
    78421 ttcttgctct gttcaaactt gaatttgccg taatccataa tcctgcatac cggaggtttt
    78481 gcctgcggcg caattttaac aagatcaagc tgtctttctg ctgccagttc caaagcctgt
    78541 ctggaagaca ttattcccag ttgatctccg tttgaatcaa ttattcttaa ttctctgtct
    78601 ctgatttcct cgttaatttg tagatctttg ttgctgctta taacctttca cctccatgca
    78661 aaattaaaat ggcaggtaga aatatccacc tgcccaaagt tttattcttg agaaccataa
    78721 ccttactgac atagtcgtaa ggtgagaagt ggaacttctt cttaaatcaa tatattaagt
    78781 tatttttctc tgctgcttta aaataataac actacgcaaa cccgatgtca acactttttt
    78841 ttggcaacgt gctatttaac cagctcatat atcgcctcgg caaacatttt cgtgcacttc
    78901 atcaagtcat caacttcaat atattcatcc ttctgatggg ctgtttcttc ctttcccgga
    78961 aataacggac cgaatgcaac agcgttcttt atggctcttg catacgtgcc cccgccgatt
    79021 gatatcaaat ccgccttttg ccctgtcaca ttctcatata ccttttgaag tatcttaatc
    79081 ataaaattat ctgcgggaac atagagggga tcctggccgt ttccttcaat gaccttgatt
    79141 ccggcttcgg atgactttct tctgatgata tcatatactt ctttccctgt ctttgtgacg
    79201 ggatatctga tgtttattcc gacgcttcct ttcttatcac tcatatcgat tgtgcccaga
    79261 ttgaaaacca gattccccga gacctcgtca ttcattgcaa ctccgaagga ttttccgtct
    79321 atctccattc cgatacttga gttaagaaag ttaacgtatt cagtctgttc cgacacgcaa
    79381 agaggaagct tgcagatgca tgagataagc tgtgaaattg cgttcacacc ttttcctggt
    79441 gtagctccgt gagccgatat tccgtaggaa tttatgatac atttatcatc gcgaatctcc
    79501 atttccagct tcatgttttg ttcatttttc atatattcta cagtctttat gactctgtcg
    79561 gtaaaatcct tcttcatctc aagctcacat tcgcagtagt ccggaaccat attgaccctg
    79621 ttcccgccct gtatcctttt tattctcaga ccgccgcacc ggctgtcttc attcggttca
    79681 aattcttttt ccagattgaa tattagtatt cccatttcgc tatttattat cggaaaatcc
    79741 gcatcaggtg aaaacccgca catcggagcc tccgcatact tgaagtagtg cttcatgcag
    79801 ccccatctgc cgctttcttc atcacagccg attattatcc tgactctcct gttgaacttc
    79861 acacccgaat ccgccaccgc tttcaatgca tatattgccg ctgctgcggg ccccttgtcg
    79921 tcaatggttc ctctgccata gattttgtcg ttgacaacat cgcctccgta tggattctgt
    79981 gaccagcctg aaccttcagg tacaatatcc acatgaacaa gtatgcctac tatttcatca
    80041 ccttctccat attcggcatg tcccgcaaag ccatcgaaat tttctgtttt aaagccaagc
    80101 ttactgcata tatccagact tctgtccagg catattctta cctctttgcc gaaaggcgca
    80161 cccggctcgg gatttccttc gatgcttctt attctcacca gttcctgagc tgcttttata
    80221 atatcatcct tcattgattc aacaattttt tcaagcttca taaaatatca cctgcctgta
    80281 gtttttttta ataagttttt tttatactcg cggaatgaat ttgctcttca ataaaaaatg
    80341 catctcacga cgcatttttt tcgctaggga accctatggt tcccttcgac ggatgcaagc
    80401 atcctgagca ccctccttta tcggcagggg gcttctcccc cttgcatccc ccatcgtata
    80461 ggaaattata atttcctata cgataaaaaa ggggctgagt tatctcaatc ccctattttt
    80521 ttaattgtcc ggggtattca gctctatcgg ctccaaggtg atgcgggctt ttctgttatc
    80581 attccaaaca tagatgccca aagccatacc gataatgccg tataccataa atgtcctgaa
    80641 atattcacct aaaagggcat tgccaaatac agccttgccg atttcaggcg acattataaa
    80701 catggaattg aaaagtatag ttccaatcaa agcctgtgat acagtagcct tgcgcaccga
    80761 agccccgcct accagtaatg ccgctactga gaacataccg atttgtgtat gtgcaccata
    80821 gacattcaga gtacccatat cctgcaagta aaatatctgt ccccaggcgg ccataacagt
    80881 ggacattata actgcaatga ttcgggtttt gtctacattt attccattgg actgggcgat
    80941 atgctgactt tggcctacac ttctaaaatc ctggccgagc tttgttttca taatcaattc
    81001 tgtgaaaagg cagagcaggc caatgaagat aatggttacc gtaggtacat ctttcacttt
    81061 tgttatgttg ttattagtcg caattgcata tgcactataa gcaattgttg ctgccagcaa
    81121 aaggactttg ataataaata taacactatt gcctttatca ggccttttcc ttcccgtaag
    81181 gtaaagggca acaaccaata ttaacaatac tgccgaaacg acgagtaccg tccacatgaa
    81241 gggcgttttc attatattct gcaaggaata gtgcagctcg cccatatcca ctgtcatacg
    81301 cactccgacg ccatcgggtt tgatcatggg atgagtagcc cggacaggta taattacacc
    81361 agttgcaaag agaagaatga actggtatac cccattggca aagaaaccga caattaagct
    81421 ggctatcatc tcctggccgc gggtcttatt gtacagcctg cctgtcagga agccgataaa
    81481 agcggcaatg ggcaatgcca aggctataca aaagaataat tcccaaactc cgctaagatg
    81541 cagatattca atgctgagat accgtacaat gattatggcc aactggccgc tcattgcgcc
    81601 gattacaata ccaaagttca gccccagtcc tgctataaca ggaattatta gagacagtac
    81661 aatgaaagca ttacgataga aacggctgga taattcagat aaaaagtaat taatgggcac
    81721 attgtcggtt agtgcaaaac ctaacaatgt gaaagcaata aaaataatag gaactatatt
    81781 atctgttaaa tacggcatct gcttgcccag catatttttg gcttttttca ttatttaccg
    81841 cctcctttga cctgtgtaag tgcgtaaaga ataataccgt tttgcagtat tacacgcatt
    81901 atctcggata agtcggttcc tgcgaacaac tgatttgcca caggcattga tgcagtaagc
    81961 agaccctgta ataggaatgt accgattata acatgagaga tattagcttt tgaggcggtt
    82021 gctccgccga tgagaacacc ggcaacagcc gggaacgcca tcatcaatgg ccctgaatat
    82081 agctggaaaa aaccataact ctgagaataa agaattattc ccagggcacc taagaccgtg
    82141 gaaagaatat tggctattac tcggttttta tcaatatcaa tacctgaggc agcggcaaat
    82201 tcaggattgc tgccgccagc gattattgca acgcctgttt tcgtcctgaa aaataaccag
    82261 acaaggaagc aaaatgcaaa aaacgtcagg agcaaacctg ttggtatctt aactccaatt
    82321 atctcaaagg aaagaaaatt gtttagaatc tctcctgcat tcaccccatc cagttttatt
    82381 gtttcacgca aacccttgcc catcatccaa cccatttgtt tgctcttaaa gggtgccatg
    82441 agccaaacca aagacatgag agcaacgatg gaaaagccgg tataagttgc gattgtcatc
    82501 tctgcgcctt ttactgcatt taaaagcctt ccgtataagt acccggttgc agccgcaaaa
    82561 accgttgcta ttattgatgt tataaggagg agcacaatac cggtaaatcc catttcaatt
    82621 cctatagtcc aggctaataa accacaaatg atgcccaagg gcagagcaaa attagggcct
    82681 gttccggatt taattgcagg aaccattgcc aaaaccaata caccattcat accaaaacga
    82741 cgcaatatat cgctgatgag catcggcaaa gattgtttca gtacgcctgc cgtaatcatt
    82801 accaataaga aaaatgcagc aataataagg gttggcaagc caatgctttt tactataggg
    82861 atatccaggt tctttttaac actattattt tgcttatctt cattgtttaa catttattca
    82921 gcacccccca tttgggaatc tgcgtgaacg ccggacatca tcaacccaaa ttcagcgttc
    82981 gaagcctcag gagacagaat gccctttact ttaccatcac agacgatagc tatcctgtca
    83041 caaatacttc taagctccgc taattcagat gatgtcatta ctatagtcat cccttgttcc
    83101 tcattaaggc ttttaagcaa gtccaaaata agcttctttg cacctatatc aataccgcgg
    83161 gtgggctcgc tgaccagcag cagtttcggt ttctgggtaa gagcccgggc catgcacacc
    83221 ttttgctggt tgcctccgga taaactgccc gcatgctgca aagaggatgt gcatcgaata
    83281 tccagatctt ttatcatctt atttgtatga ttgcggattt tttgagaatc cataacctta
    83341 ataaaaccaa gattataaag gaattccttg tggatctgca tactcgtaaa aactgtatta
    83401 agctcaatgg actcatctaa tagcagtccg acgcctttcc tgtcttcgct taaaaagctt
    83461 atccccgcaa caattgctcc cctggggtta ttcagttcca gtgggcggcc ttcaaaaatt
    83521 acttggcctc tggcgggaaa aatccccata attccgttag ctactccaag cttcccttgc
    83581 ccggccaggc ccccgatgcc tattatttca cctttcttta cctccaggtc aaaacctttt
    83641 acctcttccc cgggcatttt tacctcaaga ttcttgatgc taagcacgga ttcatcttga
    83701 atccggcggg aatccgaatc tgaacgatcc agggatacct ttcgtcccac cattaattct
    83761 gcgatttcgt acacgtttgt atcctttgta tcttttgtta ccacatgttc cccatcacgc
    83821 aaaatggtga cgcgatgaga aacttgcata atttcttcca aacgatggct tataaatatg
    83881 atgcctattc cttgtgccgc taacttctca atggcggcca acaggttttg ggtttcggat
    83941 tccgtcaaaa ctgcggtggg ctcatcgaaa acaagtagtt tgattccggt tttatcgatt
    84001 tccctggcga tttcgatgaa ctgcatatgc cctaccggca gccctgccac cagagtgtac
    84061 tcctctatac ccatgcccat aatgtcaaga gcctttctgg catcgctgtt cattcgcttc
    84121 atatccaagc tttcatattg tttgccgaat atcttactga ccaggtttgg attagtcagt
    84181 tcacggttta ttttgatgtt ttccgtaatg gtaaaacccg gtataagcat aaactcctga
    84241 tgtaccattc caattccatg gaacatagcc tcatggggcg aattgaacat gacctcttta
    84301 ccatccagtt caatgctgcc ctggtatccg ccggtttcat gaatcaccgg cataccgaac
    84361 aatatattca tcaaagtgga tttgcctgcc ccgttttctc ctatgagggc atggatttca
    84421 ccctttttta tttcaatatt gatatctttt agtacctggt tgccgtaaaa ctcttttgat
    84481 atgtttttta tagtaaggag tttgtctccc aagttaatcc ctcctgccac atatgctctt
    84541 atgagtagcg gtttgcaaac ttaatgagga tgtctcaagc cgaacagtat tgaaacaccc
    84601 tcattacaag ttatacttca gttgccagga tgcaacttaa atctttatac aacaattagc
    84661 ttggaagctt tccgtaagta aggaagtcta ccaggaacag taagtaattg tcataagtaa
    84721 cgccattttc tacatagggg ttcagagaaa tatccatgcc cgcataatca gacatgcatt
    84781 ctgccagaac ttttttgtca aacttgccgt cagtttcacc gttcaaccac ttgacggcat
    84841 attcagcgcc tgcaatagta gccatcatgg ctgccggtac gggccaagtt gagaagcgtc
    84901 cgcttaggcc ggcatcttca acgatcttcg tggtctggtc aataacatcc tgcaaaccgc
    84961 cgactatatc ttccggaaca ttcagaccca aagctgacgg gtatccgtgg taaggtgacg
    85021 ggcagcatgg ctgtgtatag attgcacctg catcaaaagc tgctttgatc agaggcacct
    85081 gcatagagca gtttgtggag aagaacgatg tatcttttcc gtatttcttt accatcttcg
    85141 gtacgtcttc caggatgaac tgctgagcac ctgtaactcc agcttcactg gttgggtccg
    85201 gagctgtagc atcgacaaat tgaagaccaa tctttgcagc ttccgctttc atgagctcac
    85261 gtctctctgc aagtatcgga atagacatat gtctcgggaa tgaataatgt acaaatgtct
    85321 ttgcgccctg ctttaatgcc tggtttggta ttgccgaacc catggacagc tcatcgggct
    85381 gaagtatgac gtctgcacgg gtggatacgt ccggagcatt ttcctgaggc gaagcataaa
    85441 caataagtac ttcgtcctta tttcttgctt ccaggaactt atcaatcgcc ggatttgttc
    85501 caggtacagc ctggttaatg actacagctt tgatatcagg atctgcagca agcttagtca
    85561 atacactgat catctgttct tgttcagtca tgaaattgtc cggccaaagc tcatggacaa
    85621 ttttatcctt gccgtatttc ttctgtacaa actcggcaga acgatactct tcttcgttct
    85681 gtgaaacagt gttggtgact attgcaattt tccaattgtc cttagcttcc tcgggtttag
    85741 cttcagaagc agcaggctct gctgctgcag gctctgctgg ttttgcgcat gccgtgaagc
    85801 taaaaagcat cacaactgcc agcattaatg ctaatgcttt tttcatttct tcccctccat
    85861 taatttttgt tatttattag accgggaatt gtatttccgg gtactaatcc ctatttacaa
    85921 aagcaaaaaa gataacagaa cattattttt tctaatacaa tgtatattga ttattctgta
    85981 aaatataaat aaatcctttt tttataacat atttctctgg aatatttttt atagcattta
    86041 ttatagcagc ttgtatttat ttttgggcct gcccagttgt ccgtatatcg gtactatttc
    86101 tatccttttc tcctcaagga gataagatac atatctatgt accgtttggt acgcaagatt
    86161 cagcccgttt gctatatcat caatcgtcag cccgtgtccc tgctccttca gataatcaag
    86221 tataatactc aaagtagcag ggctgattcc cttatcaagg taaatctgct gcttatccaa
    86281 aatatatttt ttttgaatct catttatttt aagatttatt tttatccgcg aaatcaaatc
    86341 cggtgattta ataggtttga tagcaaagtc agttgcaccc gcagcaataa atctttcagc
    86401 agtttcctgc ctctcatcga cagtcaatac cagtattgat acggcattgt ccgtctgccg
    86461 gattcttttg acagttgtca gcccatccca atccggcata tgataatcta ctatggccag
    86521 actgggcttg tgcatcctgc acagttcgta cccctgtctt ccgttggacg cagtagctgc
    86581 ttcccagcct ccatacaggc atatttcttt tatggtataa agaatatcgg gttcgtcatc
    86641 tattattaca atttccggtg tattattttt tttcaattgc ctcgcccctt ccataatctt
    86701 caaagttatc aactgacggc aggaaaatag ttacagtggt tccaaaatcc cgttgactcc
    86761 caacttctat tgttcctgca ttatcctcta taatccgctt tgcaaatggt aggcccaagc
    86821 ctgacgtatt attggttgaa tagcctatct cccatatttt agttagatta gactcatcta
    86881 tgccgattcc gttgtctttt ataattatgt tgataccctt ttctgcacgc ctcacatcaa
    86941 agtttatcat cttatatgaa tgtttacagg gaacaactat tgcattttca agtacattta
    87001 taacagccct tgctattctt atcttattta tatatatatc cggaagatcc tcctcaatac
    87061 tgatggaaaa tcttatcttt tcatcttcca gcggaagctg ggcccttata taactgataa
    87121 ggtcagatgc cttcaatttc tggcgtgaat attcatatag gaagctggaa atcatatcac
    87181 tcattttagc tactgagttt tctatacgtt cactgtattc cgccagcttt ccgtttgcat
    87241 tttcggaaac catcaaggaa ttcaagccgc gaatagtaac caaaggggtc ttcaaatcgt
    87301 gaaccaggga tttaacctct tgatatatct ggttttcgag agcctttgct ctcatgtccc
    87361 ttatctcgtt ttcttttata tgattctccc tcatcatatg aatgttcctt gaataactga
    87421 taaatagtgt tgcagtaatg aatgccgata tgataaaagg tataaaaaac gcaaaccctg
    87481 caaaatttag tacggtatcg gcattaagat atatccctga tattttcacg ctgtaaggaa
    87541 catcgcttct cccaaaccgg tatgatgaaa agtaaggcat tatattaagc cattggaaag
    87601 caaaaaagac ttgtaccgaa actatgaaaa cctgaataaa gctgtaagtt tcaacgaaca
    87661 gtttctcgaa aagaaacaat gaaataatca atgccaataa ggttgacaca ggttcccagg
    87721 gtatttcaaa tatcagcgaa tccatccaat gtaaaaatac tatactgact aatgatacaa
    87781 gaaatacatc tatggatttt aagcggctgc taagttttgt attaaaaatt aacagtatgg
    87841 atcccatata caaaagggtg ctttgaattg aacaaaggct gttcaatgat gcagatgcaa
    87901 gtatgagatg cccgctgtct ccggtggtta tcgcttctct gattttcata atagtattaa
    87961 accaggtgtt ttgaaaaaag acaggaaaca gcatgcctgt aataataagc aaaacacctc
    88021 ccgcagcaat ggtctttagt aatatgtttt catttatttt cctttggctg gcagtcatag
    88081 taaaacctct cacaaatatt atcctttatc gaaaagccga agcgaaaggt acagatatgt
    88141 ccttcacttt tcttacaaga gtcaggggta tattgccatt ctccgccaaa tcggcaagta
    88201 attcctcatt gcttgacccc ttcagcccta tgatataatc cgatttggca ccaacgagac
    88261 tgataatctc ctcatttctg acgtcaatag gctgttcacc gcctaaaaca acagttatga
    88321 ttgtaagctt attatccttt gccatattga taagcttctc aatacgtgct ttttcatctt
    88381 cgtagcttat gccctggagc tttaatccca aagaactgta acccaccgca aaaacaattg
    88441 aattcatatc ttctataccc tcagcttcag cctggggcat gaaaaagctt cttatcatca
    88501 attggtttga aatatcatta attaagtatg tatcggtact ctgtcctgca gatgtaatta
    88561 caaccgggac ttttgcaata ggccgtggca gccccggaag agtaaaaact attgtctcag
    88621 ctttcttctt ataaggcatg tttacaagaa caagcaatag ggcaagtata ataataagta
    88681 ccattctaaa tacatttcgc ttcataattc ccatcctcta cccttcatcg ctcattttca
    88741 agtgagactt gttccaagga ctcaataccg gtcatgtgag gagtatatcc cttcaccgat
    88801 agcctggcag caagaacagg cattgtgtgt accatcggta ctgaaggcat gtcatagttc
    88861 acagtttcct gaatccttct gtaaagactt ctcctgaatg acatattggt tgtctgcctt
    88921 gcttgtgcaa gcagttgatc tacatcctcg cttttataaa aggaataatt gcctgcaagt
    88981 ccgggctttg cattttcgga agccaacatt gtgtaaagga aattgtccgg atcggcataa
    89041 tctccggtcc agcctattaa agcaatttca tgctctccgt tacgtatttt tttcaaatat
    89101 tcattccagt tgaataccac aatttctgca ttaatactta cctgccgcag attttccctt
    89161 ataaactgtg ccgttttcaa tgggtctgga aaatactccc ttgaagcatt cattacccat
    89221 attgtcgtgt tgaatccatt tggataaccg gcttccgcca gcagctgcct ggatttttcc
    89281 ggattatact cgtagatttc caggcttccg ttgtaacccc agagagacgg cggtatgaag
    89341 gttgccgcag gctttgcaag attgtcaaaa acatcattga taagcttgtc cttatcaacg
    89401 gcatgattta ttgccgctct caccaatcgg ttgttaaatg gagatttctc attattcatt
    89461 gccatgtagc ccacattaaa gcttggcctg aagtacaagt acaaatttgg atcatacttt
    89521 atgcctgcaa tatcattagg gctcagataa tcagctatat gaatggtacc ctgcctcaat
    89581 tcttccaatc tatccttatt cccgggtata actttgaatt caacccgctc tacttttgcg
    89641 gcatcgttcc aatacttgtc gttgcgctca agaataatac ttttatttcg ctcccagctc
    89701 ctatagacaa aggggcctgt acctaccggg tgttcagata aatctccctg atgttttttt
    89761 atagcttcag ggcttgctat accgaatgcc ggcattgtca ttgcatataa gaaaggagca
    89821 aagggcttgt tcagttttat ttccaccgaa taattgttta atgcagtgat actttttacc
    89881 aagcccggga aaccgccgaa aatataattc caataattaa atgatccttt atgatagggg
    89941 ttatcctcat acatccaacg gttaaaattt aatacaactg catcagcatc gaaagcagtt
    90001 ccgtcatgaa actttactcc cctgcgcagc ttgaataccc atttcaagcc gtcctcactt
    90061 gattcccagc tttctgcaag gagaggcact acctcatttc ccccgttttc acatttcacc
    90121 agtgtttcca atatgtttac agttactttg aaggaatcca tttcgacagt acaggaaggg
    90181 tccaaagtaa ctgagtcatt tgcacggcct actatcagcc ttttttctgt atcgtatgaa
    90241 ccgcgtgccg catccttcaa ggttccggac atgacagcga taataattgc aaaggctgat
    90301 aatactatca atgttttatt accgattttc atgacttcac ctccacggat tatttcggca
    90361 atacatgcct gttaatatga ttgtagctta aaattcgata aaaatcaaag acaggtaatt
    90421 aacaatcaaa caatcgttat acctgccttt atttttattt ttgactcttg tatatttact
    90481 tacttattgg ttttattttt tatttcatct tgtattttag acaggaaatc ttcaaatttc
    90541 ataactcctg cgtctccttc ttttctgtcc cttaccgata cagatccgct ctccatttcc
    90601 ttgtctccta ccacaagcat gtacggaatc ttttgaagct gtgcttctct tattttatac
    90661 cctacctttt cgcttctcaa atcagcttca gcgcggattt ccagatcctc cagctgctta
    90721 ttaagcttct cggcataatc cctctgtttt tcagacaccg gaagtattat tacctgcaca
    90781 ggagcaagcc ataccggaaa tgcaccggca taattctcta tcaatatacc tataaatctt
    90841 tctatgcttc cgaatatcgt cctgtggatc ataacgggcc tgtgcttctc actgtctgca
    90901 cctatataag taaggttgaa tttttcaggc atctgaaaat ccaattgtat agttccgcac
    90961 tgccaggtac gcccgatgca gtcttccaga tggaaatcta tcttggggcc gtaaaaagcg
    91021 ccatcccctt cattaatttt atacggcagt ttggatgctt ccaatgcatt ccgaagcgcc
    91081 tcggtagcat tctcccaatc ctcatcactg cccatcgagt tttcgggccg tgtcgaaagc
    91141 tcaaccctgt atttaaaccc gaataacttg taaacgtagt ctgtcaggct tattacgccc
    91201 aggacctctt ctttaatctg ttcaggggtc agatataaat gtgcgtcatc ctgggtaaag
    91261 tttcttaccc gcatcaatcc gtgaagagcg cctgaaagct catgcctgtg cacaaggccc
    91321 aattctccca tcctgagagg caaatccctg tagctgtaca ttgcattctt gtatatcaat
    91381 atccctccgg gacagttcat gggcttaacc gcataagttt tttcatctat tgtagtaaaa
    91441 tacatattat ccttataatg atcccaatgc cctgattgat gccacaaatc ctcattcagt
    91501 attacgggag tctttatttc cttatagcca tgtttcctgt ggatattcct ccagaagttc
    91561 tcaagctcat tccttattat cattcccttg ggatggaaaa acggaaaacc ggggccttct
    91621 tcatggaggc tgaagaggtc aagctctttt cccagctttc tgtggtctct tcttttagct
    91681 tcctccagca tcttcaagta ttcttccaat tcactctttt tggtataggc agttccgtaa
    91741 atcctctgca gcatcttatt tttctcattg cctctccaat atgcccctgc cacactcaaa
    91801 agcttgagag cctttacctt tcctgtggaa ggtgcgtggg gtccggcaca caggtctaca
    91861 aattcctcct gtttgtagaa ggatattacg gcatcctcag gaaggtcgtt aataagctca
    91921 accttatagc tttcacccat cacgcccata aaatcaagag cttcacttct cggcagttca
    91981 aatctttcaa gccttagatc ctcttttacg atattctcca tttctttttc tatagccgcc
    92041 agatcttctt ccgaaaaaga tttatccgaa tcaatgtcat aatagaaacc agtgtcaata
    92101 gccggaccga ttgcaagctt ggcatcggga taaagcctct ttaccgcctg ggccagtata
    92161 tgagatcctg tatgtctcaa tgcccacttg ccgtcgtcat catcaaatgt cagaacctca
    92221 agactgcaat cttcatttac gggggtgcgc aaatctgcca ccttaccgtt cagctttgca
    92281 gccagggcgg cctttaagag cccgcttcct attgaagcga caatttcttc aactgtagtc
    92341 cctttcttat attccttaac agaaccatcc tttaaagtta ttttcaccat actgtatacc
    92401 tcctttgatt ttcagcaaaa aaacaaaaac tcccgtccct gaaggggcgg gatattttcc
    92461 cacggttcca ccctgatttg tactctggat tgtaacgtaa tcaacgtccg cgaatactta
    92521 agcttcagct tgttcaacac ggcgctctaa ggtggttttc cagcagataa tccaggaaat
    92581 gcttccagtc tgcggcattt cctccctttt ggaaccctct gctatactcg tccttatcat
    92641 agcaatcaag tatattattt gtattagatt atacattatt caaataaaaa atgcaacaaa
    92701 ggcatttttt gagcatcccg ttataacaat cttattataa ctgtctggac aaatcctatt
    92761 attccaccca gcagcccacc taacagctca atatatttca gttcttttga agtaaggttc
    92821 aatatcagtc tttccatgtt ttccagttcc agctcgtcta ttttttcttc caccatttct
    92881 cctatcttga ctttataagc cgtattgctg attattccgt caacggagct gtcaagaatg
    92941 gacattgcat ccttgtttat ctgttcgtcg aaataatcaa taatcctgct ctttatagag
    93001 taaggtatga ttgccggaat cttatagtca attaccttaa gcagcttttc ctttataata
    93061 gctataactt cctgtttgtt ttcttccgtt accagcctgt caaaaatctc atttattgaa
    93121 acaagctcct tctcaaccac attgcctatg cttttggcaa tgtcatttct tctttttgga
    93181 ataaggccct gtattgttat attcagcagc ggaattctaa aaggccttat aggcctgaaa
    93241 agcattttta tggcaagtac gtttgtaagc cagccaataa ttgccccgac aactatcaga
    93301 aaaagtgcct cgtacatttg ttctcctccc ggatgcaaag gaacatacat tcccctacaa
    93361 taaaaaactc gtctctcgag aaagcataat ttcccataca ataaaaaaag cagtataata
    93421 tgctttacgc ttattatacc acagttcaca atcacaggct atatttgttt gtttcgc
//
LOCUS       DAOQUR010000003        86978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_320_length_86978_cov_10.690784, whole genome shotgun sequence.
ACCESSION   DAOQUR010000003 DAOQUR010000000
VERSION     DAOQUR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 86978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86978
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_86978_cov_10.690784"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..599
                     /locus_tag="PKI50_01040"
     CDS             99..599
                     /locus_tag="PKI50_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT02336.1"
                     /translation="MQDNSRIVSADRDKFYKIMITRLEGLLSSESDWLANLSNAAALL
                     YTNLEDINWAGFYLMKKEELVLGPFQGKTACTRIKPGKGVCGTAAAEMRTQLVPDVHL
                     FPGHIACDSASNSEIVVPLIKDGTVFGVLDIDSPIKNRFDELDAKYLQLFADKLNKYI
                     DWSQIS"
     gene            612..2123
                     /gene="malQ"
                     /locus_tag="PKI50_01045"
     CDS             612..2123
                     /gene="malQ"
                     /locus_tag="PKI50_01045"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNT02337.1"
                     /translation="MKFGRESGILLHPTSLPSKFGIGELGSEAYGFADFLARSRQKLW
                     QILPLGPIGYGESPYQCFSAFAGNTMLISIERLIEDKLLLQEDIGPLPDFDEGLVEFE
                     KVRAFKSNLFRKAFKRFEAAGAGEDYDRFLRENDYWLNEFSFFMALKEYFGGAAWNLW
                     DKEIAFRDSKAMIYYRDKLSREIEYQSFLQYVFFSQWRLLKEYVNKQGIRIIGDLPLF
                     ISYDSSDAWSQSYLFQLDEEGNPLKTAGVPPDYFSETGQFWGNPHYRWNEMEKDGFKW
                     WRDRLDLLFQMADIIRIDHFRGFESYWEIPAGEKTAQKGCWVKAPGRKLFQTLRAHRK
                     DMHIIAEDLGFITKEVEELKNEFGFPGMKILQFIFNGPLEEELLPHNFEENSVVYTGT
                     HDNDTTTGWYLKMKIANPKADYEMKDYFHISGEIGEKAMCWTLIEAALSSPANTAIIP
                     MQDLLCLGSEARMNIPNTIGGRNWGLRLRKGMLTTELEEKLASLSELYGRNKN"
     gene            complement(2177..2367)
                     /gene="ssrS"
                     /locus_tag="PKI50_01050"
     ncRNA           complement(2177..2367)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKI50_01050"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2609..3148
                     /locus_tag="PKI50_01055"
     CDS             2609..3148
                     /locus_tag="PKI50_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNT02338.1"
                     /translation="MKYTILDFEAMIESVRKDGRIHNYEIQELKVYENYLYYFRKYVD
                     HKYHEWVEYNLFPRENIGIYKLQQSAKLQFNPSEVYKYYVDMVSVSKEGQFWKAKDLY
                     IDFIVKGDGRYYVVDIDEFNDAICRKELKENDVKNALNGLNNVLKGYYESNDLECYIQ
                     SLKEKYGGMGKLLLSKKTA"
     gene            3225..3758
                     /locus_tag="PKI50_01060"
     CDS             3225..3758
                     /locus_tag="PKI50_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNT02339.1"
                     /translation="MDLKGSKTEKNLWEAFAGESQARNKYTYFASAAKKEGYEQISGI
                     FQETADNEKEHAKLIFKFLCGIGNTAENLKHAAEGENYEWSSMYKEFEKTAIEEGFKD
                     IAEFFGKVALVEKEHEERYLALLKNLEEEKVFKREEQTTWRCRNCGYIHTGAEAPEIC
                     PTCKHPRAYFEIAAFNY"
     gene            complement(3802..4929)
                     /locus_tag="PKI50_01065"
     CDS             complement(3802..4929)
                     /locus_tag="PKI50_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNT02340.1"
                     /translation="MNLGIDGRVITWHIGSGLATYTNNLLENLNKLKQLKNIFLFYPS
                     KNGTDYFEINDIPKNLLIGERRLDFWRNVYELKWDVNYPIDVFHNTVNGIGLPNCFKG
                     VKVITLHDLIPYIMPETVDRPHLDYTLKHTPYILDDISHIITVSRHSKSDIQKYFGIE
                     DDKITVTHLAADPIFRPLDRDNAKKVIFNKYGVDKKYVLYLGGFSQRKNIARLIRAFK
                     KVIIEKEEVINLLILGEHSRSFKALWKLTEELELCDYVKFLNFVPTADLPYFYNGAEL
                     FAYPSLYEGFGLPPLEAMQCGIPVITSNVSSIPEIVGDACLLTDPYSADDISDSMLTL
                     LTDGDEWHRYSLMGIEKAKEYSWFKTAAETLEVYEKSMKGI"
     gene            5150..5296
                     /locus_tag="PKI50_01070"
                     /pseudo
     CDS             5150..5296
                     /locus_tag="PKI50_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
     gene            complement(5336..6790)
                     /gene="nhaC"
                     /locus_tag="PKI50_01075"
     CDS             complement(5336..6790)
                     /gene="nhaC"
                     /locus_tag="PKI50_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HNT02341.1"
                     /translation="MSTKTKREATLFEALIPVVITIGFLMYAVLPQFGVGQDVHVPLI
                     LGALIASIVAITRLGYTWKEVEDGIVSTISDTMQAILILAIIGMIIGTWILGGIVPTL
                     IYYGLQILSPSFFLIAACLLCCVVSLATGSSWTTAGTVGIALMGVAAGLGVNPGAAAG
                     AIISGAYFGDKMSPLSDTTNLAPAMAGANLFDHVRHMVYTTGVSLIIALIGFGILGAG
                     YAGKELDISAINEILALMSDNFVINPLLLLVPLIVIVMVARKVPAIPGLFIGTLLGGL
                     AAMIFQGASFAGVMDAMQYGVVSETGNAIIDELLTRGGYQSMMWTISLILAALTFGGV
                     LEKTGMLETIGKVILGYAKSTGALVTATIITSIFTNVLAGDQYLALVLPGKMYKDEYR
                     KRGLAPRNLSRCLEDAGTLTSPLVPWNTCGATMSTYLGVPTIQYIPYCFLNLVNPIVS
                     IVFGFTGITMMKLEDDPSAPEYKGPKSKNENVSA"
     gene            7340..7750
                     /locus_tag="PKI50_01080"
     CDS             7340..7750
                     /locus_tag="PKI50_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HNT02342.1"
                     /translation="MVKFILGVKGSGKTKRMIELANESGRTAKGNVVYIDRDRNRIHD
                     LNRRIRLIETGDFQLENLKSFYGFICGIISQDFDIENIYIDGQKIVSNAQDDCLEGFI
                     KNLEILHEKFGVDFTISCSRAIEGIPEFLKSYLI"
     gene            8030..8944
                     /locus_tag="PKI50_01085"
     CDS             8030..8944
                     /locus_tag="PKI50_01085"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HNT02343.1"
                     /translation="MFGYIKPSVQELKIKEYELFRGYYCGLCKSMGRSFGVLSRFALN
                     YDSVFLGLLLASVHGEKPVLKKESCIANPVKRKWIVKESPGIDFAADINVLLTYYKLK
                     DDIRDEGGWLPTFLKLVFHKGYKRAALTNKDIDRIIASSVSSQISLEKKGCSSTDEAA
                     EPFANMMMQLLASGYSGDDKSVPRILGWIGYNLGKWIYIIDAFDDMERDIKSGSYNPL
                     LRQYKYENQDIKDFKASIAEEVKVGLLQALSQATGSLELLKLDNKGIISNIAYEGLYG
                     KTERVLNCIAGEKRSCVRDEESIRGIGY"
     gene            8913..9542
                     /locus_tag="PKI50_01090"
     CDS             8913..9542
                     /locus_tag="PKI50_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNT02344.1"
                     /translation="MRNPYEVLGIREGTSVEGIKRAYKELVRKYHPDQYQNNPLSDLA
                     EEKLKEINEAYDYLMKKAESEERNSSYRQQEEPRYGNNRENGDRGLYDQARIYVNMGN
                     TAAAEEILNRISFRGGEWYYLRGLIFMRRGWYNEAVTCLKTAVDMEPSNYEFRDALNS
                     IMNANRQYQSTVYERRGYTSGPDLCTLCQCMICTDCCCECMGGDFIRCC"
     gene            9647..10162
                     /locus_tag="PKI50_01095"
     CDS             9647..10162
                     /locus_tag="PKI50_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02345.1"
                     /translation="MSRKMAFKGIFAGIAVVFLYLSTIMPTGKLTLYFLASLPVAITV
                     AELGAGAGIAVYFAASLLNILITGNITGAVPFVFFFGHYPVFKYFIEKGRKAAVELLL
                     KLTVFNMSIFLWYLFFKNLFITALPAKFTGGSILTAVFITAMQAVFLVYDYVFSRLLF
                     YYKSKLSLFRR"
     gene            10208..10624
                     /locus_tag="PKI50_01100"
     CDS             10208..10624
                     /locus_tag="PKI50_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HNT02346.1"
                     /translation="MKGYKWWDMNSSIRIIRKSEGKVSTWSGGTTTQLYIYPGNAVYA
                     DRNFRWRLSSAKVAADESTFTPLPGISRILMIIEGEVEIEHQGHHRTVLKAFEKDSFS
                     GDWTTICKGKAADYNLMTDKSCSDKKINIIRTTIYY"
     gene            10760..11203
                     /locus_tag="PKI50_01105"
     CDS             10760..11203
                     /locus_tag="PKI50_01105"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNT02347.1"
                     /translation="MDKREEALEKVMVYFNGGYNCCESVVMAVCDYLGADKDIPQKIA
                     TPFGAGMSKNGSNCGALNAAFICMGLVGGRRSSEDSKDTSFLPADRIFGKFKEKYGST
                     FCYDITGLDMKKTAETEEEKAKRHNELCGPLVLQVTQWILEELNP"
     gene            complement(11341..12270)
                     /gene="fba"
                     /locus_tag="PKI50_01110"
     CDS             complement(11341..12270)
                     /gene="fba"
                     /locus_tag="PKI50_01110"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNT02348.1"
                     /translation="MPLVTSKEMFRKAYESQYAVGAFNVNNMEIIQGIVDAAKEENAP
                     LILQVSAGARKYAKHVYLMKLVEAAVEDTGLPICLHLDHGEDFEICKSCIDGGFTSVM
                     IDGSKHPFEENIAVTKRVVEYAHERGIAVEAELGKLAGVEDTVNVSAKDATYTDPDQA
                     VEFVERTGVDSLAIAIGTSHGAYKFKGEPKLDFERLEKITKLLPGFPLVLHGASTVLP
                     EFVELCNKYGGNVAGAQGVPEDMLLRAGKLGVCKINIDTDLRLAMTASIRKHFAENPG
                     DFDPRQYLKPAREAIKNMVKHKMRNVLNCSGRM"
     gene            complement(12359..13393)
                     /locus_tag="PKI50_01115"
     CDS             complement(12359..13393)
                     /locus_tag="PKI50_01115"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNT02349.1"
                     /translation="MAEIRIMRTSDCKPGMVLASDIINDYGAVILYQNSILDEYSISK
                     LMKLGLNFVKIYKDYEFKEKKHAVIEAQYNNNLEEFKEIIWDISCGKTLEMERVSEVS
                     RNISSNFDSIHDLVSCLSKVRSISEYTYAHSLNVSLLCSLLGSWLNLGHKHIEQLSYC
                     GILHDIGKSKIPPEILNKPDTLTEEEFDKIKQHPLIGYKMLEDNKEISKDVALGVLMH
                     HEREDGSGYPFGFKSERIYYYAKIVAVVDTFDAMTSNRIYKKRQTPFDVLEMYESEYL
                     TKCDAGIMLTFLKHISSYYIGAKVRLSDSSIGEIVYINTNRISRPLIKSKETIIDLSM
                     VPELKIVEML"
     gene            13672..14205
                     /locus_tag="PKI50_01120"
     CDS             13672..14205
                     /locus_tag="PKI50_01120"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNT02350.1"
                     /translation="MLTLNNKLDNLIFRDITKEDLKAVLNLYNQNDQNIYATGIDRRM
                     SLRDIKEKYLEVLVNSHEFFTGIFLEKEDALQLVGVIKGRIDYENSEEAWISSILVDN
                     RYQRLGIGTKAAEALINMLNKSYDVKRFYIGIIARNSVGKCFWQKLEFSYFRTIEQYI
                     KLNALAEDFIIMKREVL"
     gene            complement(14256..14789)
                     /locus_tag="PKI50_01125"
     CDS             complement(14256..14789)
                     /locus_tag="PKI50_01125"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT02351.1"
                     /translation="MSSILSGILRQKLLEIQNQLPPYVKIIKDNPPSFEDVLNKEINE
                     QSDVQNNYGGDYSHLIKAAAKKYNINENIITAVMKAESGFDPTAVSRTGAMGLMQLMP
                     ETAEALGVHNAFDPRENIEGGVRYLKDMLTEFGGNLELALAAYNAGPNAVKKYGGIPP
                     YEETQNYVRKVMSSLKR"
     gene            14963..15313
                     /locus_tag="PKI50_01130"
     CDS             14963..15313
                     /locus_tag="PKI50_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNT02352.1"
                     /translation="MKICEVKEVLNAELLCGDELLENEVSYAFGSDLMSDVLAFVKGK
                     TLLLTGLTNQQVVRTAEMADLSAIVFVRGKKPGEDIVQLATEKDIALLLTRDTMYTAS
                     GKLYSNGLEGVSID"
     gene            15361..15801
                     /locus_tag="PKI50_01135"
     CDS             15361..15801
                     /locus_tag="PKI50_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT02353.1"
                     /translation="MEDIKYVFDVKKDDFVRAGETSSKVKKILRELGIDSSIIRKISI
                     AAYEAEMNIVIHSTGGQIVLEITPGIIRITANDRGPGIADVELAMKEGYSTATDKVRE
                     MGFGAGMGLPNMKRCSDRLYVTSSEGTGTLIVIEVDLKAGEQGE"
     gene            15803..17083
                     /locus_tag="PKI50_01140"
     CDS             15803..17083
                     /locus_tag="PKI50_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT02354.1"
                     /translation="MKEKYFHSVVLDADRCVGCTSCLKVCPTEAIRLKEGKACIIGEK
                     CIDCGECIRICPNHAKNAVTDSKDILKKYKYTIAIPSLVLYGQFRGNVGINEMLTAVR
                     KIGFDEIFEASAGTEIITNVIKNHILKNPEKKRPIINSSCPAALRLIQIRFPELLPNI
                     ADIETPVEIAARLAKAEAVRRTGFSMEEIGVIYISPCTARMTSVKHPIGIKKSYIDGV
                     LSMKDVYGMMLRNLGSNTEDLTRSTKESLLWPIIGGQGKPLGIKNYLAVDGIHEVIKI
                     LEEIEMDKLEGLEFFEGMACVGGCVGGPLTIENPFIARRRMKTLAKGLPDSNPSDEEI
                     KGQIEMYKSGFIRLTEAIEPKAVMRLDDDICKSIKKMEKIKEMLKDLPGIDCGVCGAP
                     TCKAFAEDIVKGENRNAVCIVKTMKSFNKEKPQS"
     gene            17095..17430
                     /locus_tag="PKI50_01145"
     CDS             17095..17430
                     /locus_tag="PKI50_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT02355.1"
                     /translation="MLVRDAGEKIGLKLLAGEAGTSREITGVYICDLLSWVMAHAGKG
                     DAWITVQTHANIVAVAALLELGCIIIPEGVEVEEDTLRKAEDEGVPVYQSGLSAYGIA
                     KELYRMGIE"
     gene            17432..18145
                     /locus_tag="PKI50_01150"
     CDS             17432..18145
                     /locus_tag="PKI50_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT02356.1"
                     /translation="MEFAVDFHIHSALSPCGDEDMTPCNIVNMALLKGLDIIAVTDHN
                     SCGNLPAVMEAAKENGLMVIPGMEVQTKEEVHLLCLFKRMEGAMKFAEIVYNSLPDIE
                     NNEEIFGRQLIFNSSDEIIGKEHKLLLSSTILSVNDVFILTRGLGGVCIPAHVDRQGF
                     SIITNLGFIPPDLKVKTVEISKRCTPDAAFKKYPFLSRFNYIISSDAHYLEDISERDF
                     FIELDCLTVSELFNKLDPK"
     gene            18427..18897
                     /gene="nuoE"
                     /locus_tag="PKI50_01155"
     CDS             18427..18897
                     /gene="nuoE"
                     /locus_tag="PKI50_01155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT02357.1"
                     /translation="MSDAAMKENMEKLEDFIQKHKNQKGALIPILHEAQELFGYIPEE
                     VQKRIAEVLHIPAAEIFGVATFYSRFTLTPRGKHTISVCLGTACYVKNSKGVLERLEE
                     ELNVKTGGTTADGKFTLEATRCLGCCGLAPVMMIGEDVYGKLVPDDIPDIVKKY"
     gene            18905..19468
                     /locus_tag="PKI50_01160"
     CDS             18905..19468
                     /locus_tag="PKI50_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT02358.1"
                     /translation="MKDLSLHILDLSQNSISAGAGLVEISIEEDTKEDRLTICINDNG
                     KGMDKDFLEKVMDPFVTTRTSRRVGLGIPLMLAACSRCEGDLVVESEKNVGTRLKAVF
                     KHSHIDRAPMGDMAETMLSLILAGSDVNRNVDFVYRHIIDGRVFCLDTREIRTALGND
                     INLGEPEVLMWIKDYINEGLKNIRGGV"
     gene            19470..19838
                     /locus_tag="PKI50_01165"
     CDS             19470..19838
                     /locus_tag="PKI50_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNT02359.1"
                     /translation="MKTIQELEEIRKKTLDTINIRKDRSGTRIVVGMATCGIAAGARP
                     VLLTIMDEIKKHDLSDVVVAQTGCIGMCRLEPIVEVYKPGEEKVTYVKVNPSKAIRIV
                     NEHIMKGKVVEGYTIGAESK"
     gene            19892..21685
                     /gene="nuoF"
                     /locus_tag="PKI50_01170"
     CDS             19892..21685
                     /gene="nuoF"
                     /locus_tag="PKI50_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNT02360.1"
                     /translation="MEMYRSHVMICRGTGCTSSNSEQIAKNFEEEIKKHGLEKEVVVE
                     RTGCFGLCALGPVVIIYPEASFYSMVKPEDVEEIVKEHLVKGRIVKRLLYAESVEENR
                     LKALEEVDFFKKQVRVALRNCGIINPENIDEYIAFDGYKALEKVLTKMTPAEVIDTVK
                     RSGLRGRGGAGFPTGMKWEFASKSVNDQKYVLCNADEGDPGAFMDRSVLEGDPHSILE
                     AMTIAGYAIGASQGYIYCRAEYPIAVKRLGIAIGQARLYGLLGDNIMGTGFKFDIELR
                     LGAGAFVCGEETALIASVEGQRGMPRPRPPFPANKGLFAKPTILNNVETYANIPVIFN
                     KGPEWFASMGTEKSKGTKVFALGGKISNTGLVEIPMGTSLREVIYEIGGGCPNGKKFK
                     AVQTGGPSGGCIPEEHLDSPIDYESLAALGSIIGSGGMIVMDEDNCMVDIARFFMDFI
                     VDESCGKCTPCREGTKRMLEILERITEGKGEEGDLDKLESLAKNIKNAALCGLGQTAP
                     NPVLSTIRYFRDEYEAHIKDKKCPAGVCKELLDYFIIADKCKGCGICAKNCPQQCISG
                     QPKTPYTIDSSKCIKCGVCMQKCPFKAIIRK"
     gene            22065..23396
                     /gene="rsxC"
                     /locus_tag="PKI50_01175"
     CDS             22065..23396
                     /gene="rsxC"
                     /locus_tag="PKI50_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNT02361.1"
                     /translation="MRTLTFKGGTHPPHSKKATESLPIERANEPKVVVIPMQQHIGAP
                     CEPIVQAGDEVKVGQKIGEAKGFVSVPVHSSVSGKVAVVEPRLYSGGTAVPCIVIEND
                     MQNTVSDQVVPKGDISVLSAEDIKNIIKEAGIVGMGGATFPTHVKLAPPPDKNVDTVI
                     LNGAECEPYLTSDHRLMLEHPENVVFGLQALMKALGVKKGFIGIETNKPDAIEKVFEA
                     ARGIEDIEVVALKTKYPQGAEKQLIYACTGREVPSGGLPADAGVVVNNVGTAAAVATA
                     IKTGMPLIERIVTVTGEGVNNPKNLLVKIGTSFRDIIEQCGGLKGKVGKIIAGGPMMG
                     ITQFSLDIPAIKGTSGILVLSEEEARLPEPSNCIKCGKCVETCPINLMPVNISMYSLA
                     NRYDEAEALNAMDCIECGACSFVCPAKRPLVDSIRVAKREILARRKKAQSK"
     gene            23411..24406
                     /locus_tag="PKI50_01180"
     CDS             23411..24406
                     /locus_tag="PKI50_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNT02362.1"
                     /translation="MENSRFLVSSSPHIRSGETTQRIMLDVIIALMPALLAGVFYFGL
                     NALTLTAVAVVFAVTTEAVMQKILGKPVTVNDLSAVVTGILIAFNVPVTLPLWMAAVG
                     SIFAIAIAKQCFGGLGYNFINPALAARAMLLASWPVRMTAWVAPGTDAVSTATPLALV
                     KMGADALSGATHWEEGVEAVGQTLPGLWDLFIGNIGGCIGETSALALILGGAYLVYRR
                     VISIRIPVSYIAAVAVFTLLFGRFDFAAAIYHVLAGGLFLGAIFMATDYSTSPITPKG
                     QIIFGIGCGFLTSVIRFYGGYPEGVSYSILLMNLATPLIDKYTMPRKFGEVKKSA"
     gene            24399..24983
                     /locus_tag="PKI50_01185"
     CDS             24399..24983
                     /locus_tag="PKI50_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNT02363.1"
                     /translation="MRDYIRLAGILLLVCVVMAASLGYTNAITYDRIQEQIIKANDEA
                     RKEVLPDADAFEILDDNAFSEIKSNGKYNFVTEIYTAKAGGNVVGYAVKAVPKGFGGT
                     IDVVVGIGTDGIIQGIKVGNNTETPGLGKNSATPKFQNQFKGKAWDNTINVIKSGTPK
                     DNEIAAVSGATITSRAVTDGVNQALEAAKKLSGK"
     gene            25006..25602
                     /locus_tag="PKI50_01190"
     CDS             25006..25602
                     /locus_tag="PKI50_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNT02364.1"
                     /translation="MNLWKDFSNGLIKENPTFRLVLGTCPTLAVTTAVFNGIGMGIAA
                     AAVLIGSNLIISLIKKIVPNEVRIPIYIVVIATFTTIVQMLIQAFSPALDKSLGIYIP
                     LIVVNCIIIARAEAFAAKNPVANSIMDGLGMGIGFTVALTIISSVREILGAGTFLGMP
                     LFGEAFEPFLIMILPPGGFLVFGLSIAAFNKITSLRKS"
     gene            25617..26201
                     /gene="rsxA"
                     /locus_tag="PKI50_01195"
     CDS             25617..26201
                     /gene="rsxA"
                     /locus_tag="PKI50_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNT02365.1"
                     /translation="MKLGLIIISAIFINNFVLSQFLGICPFLGVSKKIDTAVGMSIAV
                     TFVMGLASVICWAVQIFVLDPMGIGYMQTIAFILVIAGLVQFVEMVVKKTSPTLYQAL
                     GIYLPLITTNCAVLGVAILNIDKSYNLIESLINGVGAGLGFSLAIVLFAGVRERLELA
                     PIPKALEGFPIAMIAASLFSIAFLGFSGLFSGLL"
     gene            26235..27212
                     /locus_tag="PKI50_01200"
     CDS             26235..27212
                     /locus_tag="PKI50_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HNT02366.1"
                     /translation="MFNSIGLPVIVLGGMAVFFGIVLAYASKKFAVEVNPKVTEIRDV
                     LPGANCGGCGFAGCDALAEAIASGNAPISQCPVGGSELAAKLAVIMGVEAGSEERQVA
                     RVICCGDSNNAAEKYEYQGIKDCKAAEMLAGGSKSCRYGCTGLGTCVRECPFDAIHIV
                     NGIAVVDEEKCTACKKCIAVCPKGIIELVPVSKKVRVLCRNQDKGKATMQACKIGCIA
                     CQKCVKACKFDAIKVENNVARIDYSKCVNCMMCAEVCPTKTIYANFADRKKAFIIEDK
                     CIGCTICSKNCNFEAIEGELKGKHKVLDDKCVGCGQCFAKCPKDAIEMK"
     gene            27635..27886
                     /locus_tag="PKI50_01205"
     CDS             27635..27886
                     /locus_tag="PKI50_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNT02367.1"
                     /translation="MRREGGRNGWIMLLMVVCGLVVGGFLGELLGKYFPILKYGYNLG
                     VSAHEWNFHILRLTFGFDFNINMFSILGIVIAIYIYRKL"
     gene            27915..28484
                     /locus_tag="PKI50_01210"
     CDS             27915..28484
                     /locus_tag="PKI50_01210"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNT02368.1"
                     /translation="MRIILASNSPRRKELLSQTGIKFEVIPSSFEESIAELPASKLVE
                     HFAYMKAKDVAKSVKGDALVIGSDTIVYCDEIMGKPRNREEAFCMLTKLSGKQHLVIS
                     GISIINTATGESLTEHESTRVQIKELSSAEITAYINTGEPEDKAGAYAIQGLGSLFVE
                     GIQGDYFNVVGLPIFRLRKMLEHFGVKLI"
     gene            28540..29229
                     /gene="radC"
                     /locus_tag="PKI50_01215"
     CDS             28540..29229
                     /gene="radC"
                     /locus_tag="PKI50_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNT02369.1"
                     /translation="MQRGHATIKNLPEEERPRERLARHGASVLSNAELLAILLRTGTR
                     EESAISLAHRILVQEQGLRYLADSNVEQLSAINGIGKAKAAQIKAAIELGKRLAAFEP
                     GADKPLKCPQDVAGLLMEEMRYLKKEHMKLVLLNVKCNLISVEEISVGSLNASIVHPR
                     EVFNPAIRKSSASIIMVHNHPSGDPAPSSEDISITARIAEAGKLIGIELVDHIIIGDG
                     KYVSMKEKGLF"
     gene            29371..30399
                     /locus_tag="PKI50_01220"
     CDS             29371..30399
                     /locus_tag="PKI50_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT02370.1"
                     /translation="MGLFNFLSKDIGIDLGTANTLVYVRGKGIVVREPSVVAMKLDTN
                     AVMAVGDEAKKMIGRTPGNIIAIRPMRDGVIADFNVTHRMIKYFIVRAYAGSPVGKPR
                     VLVSVPSGITEVERRAVEEAAMQAGARIARLIEEPMAAAIGAGLPVGEPTGSMIVNMG
                     GGTTEIAVISLGGIVTSKSIRAAGDKQDEAIVHYIKRMYNLAIGERTGEEIKIVIGSA
                     YPNTAEGSMDIKGRDLVTGLPKTIKVTSEEILEALREPIAIILDGIKSTLEKTPPELA
                     ADIMDRGIMLSGGGALLHGLDILVKHETGMPVQVAENPLDCVAIGTGKALEQYDEISK
                     SYSGKHRR"
     gene            30422..31231
                     /gene="mreC"
                     /locus_tag="PKI50_01225"
     CDS             30422..31231
                     /gene="mreC"
                     /locus_tag="PKI50_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNT02371.1"
                     /translation="MKIRNKLLISVVLISILLISLIAVTSQGRTRPGVIDGIFGRIIT
                     PVQRVFYSSGEFFKNTFKSLYDLKDLKTENENLKAEVEKLKAQNRQLIQMALENGRLR
                     SLLEFKDTNTQFEYIGANVTGRDPGNWYDVYTIDKGSGDGIQVNDAVIVGHGYLVGKI
                     IETGANYSKLMAIIDERSSISIIVNRTRDMGIVSGSADSDVIAIMELEADIVKGDDII
                     TSEYSTLPKGLHIGKVKSVEKQEKKLQKVVIIEPSVDFKRLEEVFVIKASK"
     gene            31249..31761
                     /gene="mreD"
                     /locus_tag="PKI50_01230"
     CDS             31249..31761
                     /gene="mreD"
                     /locus_tag="PKI50_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNT02372.1"
                     /translation="MRIAIICILAVFNVILESTLFQCTRINGIKPDFSIMIIVAYAIM
                     RGSSFGTFIGLGIGLLIDVMYARVIGINALSYMVTGYIIGQAHEKVFKDSFIPPFIFN
                     LIAIIILQHSFLLASYFSNDFPSAGSSYIYMLLQVILPQALYNAIAGSIVYRFIYKLD
                     ETSFMDRRIY"
     gene            31807..34248
                     /gene="mrdA"
                     /locus_tag="PKI50_01235"
     CDS             31807..34248
                     /gene="mrdA"
                     /locus_tag="PKI50_01235"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNT02373.1"
                     /translation="MFKKYFSNRLDIIRAVTVLVFLVMAFRLADLQLVKGEYYRKKSE
                     TLRTRNISVTAPRGHIVDKFGRLIAGNKQSYSVNIMKTDVPQDTLNDIALSVINIIEQ
                     NGDTYKDEIPILINPIRFSYQDDEINWKKKYGIPESASAKDAFMKLRFDYEIPADTID
                     LEAYEIMRDEKSVELPFDINEFEFSFRKSEIKWKQSHGFGPEKSASEIFDVLCSKYKI
                     PRDIYDDEKAKKILAVKYLVGQNRYKAYEPVEIAANIKMETRAQIEESKIFLPGVEII
                     QKPIRSYAGRDFASHIIGYMGMIGSELEELASKGYTPQDLIGKSGIENSMEEYLKGKD
                     GSRQIEVDVNGTLINTINEIDPVPGDTVFLTIDAKLQKTAEESLARTMENIRNGIVKG
                     KPYPNATSGAAVAIDVNTGKILALASEPRFDPNLFAAGISSEDWKNLQPKSKDVYAPK
                     PLMNNAISSIFSPGSTMKLVSAVAGLESKVITPSETIYDKGYYTAIPGVSPSCSIFKS
                     TGIPHYSQNVSQAIKNSCNYFFFEVGRRMGGEVFEEYAKKFGFGEKTGIELLSEYKGT
                     VEGPTNKKELFRRYLDNYLVNNLQINDKNVRDEIKGYLDNNPGESKVRKRLKELGIND
                     SKAIERVWRYIRDSKYQPGDVLNATIGQGMNGVTPLQLANAVASIVNGGTRYRPYLVD
                     KVIDYDGNVKLEKKPEVAEKNDISPANLEAIKRGMYAVTNEAGGTARSAFTGSKVIIA
                     GKTGTAEVGKGYDAHAWFAAFAPYEKPEIAVVVMITQGGHGNYPAPVAREIIEAYLAP
                     EATKDNMHIDNEILP"
     gene            34349..35014
                     /gene="minC"
                     /locus_tag="PKI50_01240"
     CDS             34349..35014
                     /gene="minC"
                     /locus_tag="PKI50_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNT02374.1"
                     /translation="MAENAVIFKGTKDGLYIILKEEMDLSTIKYNLEKKIKPSKRFFE
                     GANIMNFKGKKLTQDEFDELKEIMEQEYGMTVIGSYNDKDYPVHEEHRGAKKPEILEQ
                     LPFDNVHKGEALIVRATLRSGQLIQYKGDVVVIGDVNPGAHIKSAGSVIVMGTVRGII
                     QAGANGDYGAFIVAYKMQPSQLRIGDIITRSPDGMGSKSDNPEIALVKQGMIVVEPYL
                     KNR"
     gene            35063..35869
                     /gene="minD"
                     /locus_tag="PKI50_01245"
     CDS             35063..35869
                     /gene="minD"
                     /locus_tag="PKI50_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNT02375.1"
                     /translation="MGEVIVVTSGKGGVGKTTTTANIGTGLALKGKSVVLVDADIGLR
                     NLDVVMGLENRIVYDLVDVVEGVCRQRQALIKDKRFPNLYLLPAAQTKDKNAVNPEQM
                     QKLTGDLAEEFDYVILDCPAGIEQGFKNAIAGANKAIVVTTPEVSAVRDADRIIGLLE
                     ASGLRNPMLIINRIKMDMVKRGDMMNIDDMIDILAIDLLGVVPDDDLIVVSTNRGEPV
                     IAIEQSLAGQAYRNIVRRVLGEDIPFMNLESEDGFFKRVAKLFGLNKKKQ"
     gene            35885..36166
                     /gene="minE"
                     /locus_tag="PKI50_01250"
     CDS             35885..36166
                     /gene="minE"
                     /locus_tag="PKI50_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNT02376.1"
                     /translation="MDLFKWFNRSTSSKDIAKERLKLVLIHDRANVSPQFLEMIKGEL
                     IKVISDYMEIDETGLEIKLTRTKREIDDSTVPALVANIPIKRMKGNVNI"
     gene            36336..37070
                     /locus_tag="PKI50_01255"
     CDS             36336..37070
                     /locus_tag="PKI50_01255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT02377.1"
                     /translation="MGNNNFENIPRPRYVKEYRKLGAAFKTRRYGERNFGERFLMQSF
                     ISLAAIAAVLLVNNINIGIANTISDGIRTTINWNMDFSKVLGTFRNVGDIIPEAKENL
                     GVAQVGIESFEDSPSFIFPVEGEITSEYGERIHPVFKTVRMHTGIDIDAAIGTPIKSS
                     AAGTVTTVGEDETNGKYVSIRSGKYDVIYAHCYKITVKEGQKVQQGEIIGETGDTGIT
                     SGPHLHFEIQEAGKPVSPMEMLMEVQ"
     gene            37104..37943
                     /locus_tag="PKI50_01260"
     CDS             37104..37943
                     /locus_tag="PKI50_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNT02378.1"
                     /translation="MRIKLNVFFILFLFVSCIIGWLQQSLIMFASVLLHEFGHVLTAK
                     KSGIKVYEVELMPFGGISRMEELSKLGGAAEAVVSAAGPVTSFVLSLTFSFFREYSGV
                     LESACRYNLIICLFNLLPVIPLDGGRIARNLLCFLMGYRQATKILSSAGKIAAFLLLG
                     FNIHMLAAGSKSGALIIAAVFIYIGALKEEKNSSYYYLFTGNNTKNAMIARGRIRKRF
                     IKVQEDNLIRQIVNKFSPVTQCCVEVADAKGRLKRILSEEEIIKGLLQYGYDGKIAQI
                     ING"
     assembly_gap    38006..38105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            38427..40283
                     /locus_tag="PKI50_01265"
     CDS             38427..40283
                     /locus_tag="PKI50_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNT02379.1"
                     /translation="MKYDNTREFIYEELLSKVEKPGRYIGTEWNSIHKNPETVDIRFA
                     FCFPDVYEVGMSHLGMKILYHLLNDQQDIFCERVFAPWPDMEEVMRKTGIKLFGLESR
                     DFIDSFDFVGFTLQYEMSYTNIVNMLDLAGIPKRSGERNEGHPFVVAGGPCAYNPEPL
                     YDIVDFFMLGEGEEQLLEVMEHYRKWKDSKGTREEFLKMAASVRGVYVPKFYNVEYNE
                     DSTIKSIKPIKEDIPGKVRKRVIKNLKGSYFPDKIVVPFIDIVHDRIMLEIFRGCTRG
                     CRFCQAGMIYRPVREREVEELLEIADKLIQSSGYEEISLSSLSTSDFSKLNELVERLV
                     SRYKSSRIGLSLPSLRIDSFSLKLVQEVQKVRKSGLTFAPEAGTQRMRNVINKGVDEE
                     DLLNSVGAAFETGYSSVKLYYMIGLPGETMEDIQGIADLTFKVVDRYYQVPKEKRGKG
                     LNVTASASSFVPKPFTPFQWEPQDPINTLIEKQRYLQKAIKKKQITFNWHEPYVSYME
                     AVFARGDRRLCDVLIRAWELGCKFDGWDQYFDFEAWLQAFKDTGADPDSYAARRRELD
                     EVLPWDHIDVGVTKKYLRGEYKKSLKGELTRDCRTACTGCGINLLQEGGVCK"
     gene            40280..40993
                     /locus_tag="PKI50_01270"
     CDS             40280..40993
                     /locus_tag="PKI50_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNT02380.1"
                     /translation="MMHIRLKFTKGNEVKYISHLDLMRVFQRALRRTGIPISYSSGFN
                     PHMEISFGAPLSLGVTSNAEYVDLKLAEPMDVREIQRRLNDSLPDGIRILDGIEIREN
                     HKSAMSVVSHSRYRIRIFMENVSSDELTKKIEEFAAQKEIKIMKEQPKKGFELKEIDI
                     KPLLVSMKLIESKEDIYTIDCLLLSGSRGNLKPGLLIKAFKEFTGYDIQSIRINREQV
                     YGLKNGKLLDLLEYESAEA"
     gene            41029..42552
                     /locus_tag="PKI50_01275"
     CDS             41029..42552
                     /locus_tag="PKI50_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNT02381.1"
                     /translation="MKEIIIDVGSRQTRVALLENKELTEIYIERQSEERITGNIYKGR
                     VENVLPGMQAAFINIGLDKNAFLYVKDALPNTYFDEDEEYVSSAQYKDYQIDELLKAG
                     QEIMVQIMKEPIDSKGARVTTHITLPGRYAVLMPTVEYIGISRRIEDEEIREKLKKLA
                     EELKPKGMGLIIRTAAEDCTEADLSNDINFLLKLWDNIKQKQKTGAAPRIVHKDMNII
                     YRTVRDMFTKDIDRLVINNKEHYEKVRELVTIISSHFKNKVEYYNKSYDIFEYYQAEP
                     QISRIINRKVWLKCGGYIVIDQTEALTSIDVNTGKYVGTVDLKDTVLKTNIEAAKEIA
                     KQLRLRDIGGIIIIDFIDMADEIHESTVLEALKNALKKDRTRTNVLGITQLGLVEMTR
                     KKVRQRIEKILMAPCPYCKGEGRILSSETIVKKIEKEVNSLFFNTGAMAVFLEIHPDV
                     EELINYYIDEDMFELGKSFNKPVFVRTSAELHREGYIVKGIGMSEITELRKKGCREI"
     gene            42683..42994
                     /gene="rplU"
                     /locus_tag="PKI50_01280"
     CDS             42683..42994
                     /gene="rplU"
                     /locus_tag="PKI50_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNT02382.1"
                     /translation="MYAIIKTGGKQYMVKAGDVLAVEKLDAESGEKVVFEEVLAVGDN
                     GELQVGNPVVAGAKVEGTVVGNGKAKKVIVFKYKPKKDYRRKQGHRQPFTKVKIEKIN
                     A"
     gene            43024..43356
                     /locus_tag="PKI50_01285"
     CDS             43024..43356
                     /locus_tag="PKI50_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HNT02383.1"
                     /translation="MIKIVIYKAREGKITGFKISGHSGYGTHGTDIVCSAVSALGQTA
                     LLGLLKVAEAEVHYRIEEGYLDCSIAGAGSDRKRIMCDAILETMYEGFKSIKDSYKRH
                     IDIVEEEV"
     gene            43359..43640
                     /gene="rpmA"
                     /locus_tag="PKI50_01290"
     CDS             43359..43640
                     /gene="rpmA"
                     /locus_tag="PKI50_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNT02384.1"
                     /translation="MFKMNLQLFAHKKGVGSSRNGRDSHAQRLGTKKADGQSVLAGNI
                     IVRQRGTKIHPGINVGIGKDDTLFALIEGVVKFERKGRDKKQASVYPVQ"
     gene            43716..44396
                     /locus_tag="PKI50_01295"
     CDS             43716..44396
                     /locus_tag="PKI50_01295"
                     /inference="COORDINATES: protein motif:HMM:NF026040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HNT02385.1"
                     /translation="MSIKNSGIYDVPDIESEKERYIESLKEHLSEQRHDFFNLLQVLY
                     GYTQLKKPDKVLEHISEYCKRMENIGRLYNCKCIKLADLLYNKVKEAESADLKLEVSV
                     EVSFDSAVRILDEVPVVYVVDHVIMGFLYVLDEKGMKNAHIIYNLKEISEGFLMEIYC
                     REIRKGLFGPVDFIFPEKVIYWKKAARGVLGFDKIMEYCSDVKLDGKVLGDGTTFILS
                     ISKVNKGE"
     gene            44398..45672
                     /gene="obgE"
                     /locus_tag="PKI50_01300"
     CDS             44398..45672
                     /gene="obgE"
                     /locus_tag="PKI50_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNT02386.1"
                     /translation="MFVDKAKVYVKAGNGGDGRVSFRREKYVPNGGPDGGDGGKGGDI
                     VFIADENMRTLMDFRYKRKHIAENGEMGGTNNSSGKSADDLYIKVPAGTILRDAESGL
                     LLGDLKNPGDKAIVALGGRGGKGNQHFATPTRQAPRFAQPGEPGMERWIELELKLLAD
                     VGLIGFPNVGKSTFLSVVTSAKPKIANYHFTTLVPNLGVVDLGEARSFVIADIPGLIE
                     GAHSGVGLGHDFLRHVERTKVLIHLLDASGLEGRDPLEDFDKINEELRLYSEKLANKP
                     QVVACNKMDIPAARENYARVAETLSRRGYEVFAISAATRDGIAPLMDRVFNLLESTKD
                     EEPVEEIVDLMYHINTEDKPFTARKENGVFIVEGKLIEQLLASVNLDDNDSIKYFQKV
                     LRKRGIVDELRQLGVKDGDTIRMLDLEFEYFE"
     gene            45697..45882
                     /locus_tag="PKI50_01305"
     CDS             45697..45882
                     /locus_tag="PKI50_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02387.1"
                     /translation="MNCMHCENCKQNTATYFCLAKNEIIINDSFVANIEKSRKGWKKG
                     DPGYETHRRKLRKEIEV"
     gene            46172..46321
                     /locus_tag="PKI50_01310"
     CDS             46172..46321
                     /locus_tag="PKI50_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02388.1"
                     /translation="MNNLLGKGISGIYYFSFNLGYYFSAGYYACKKEYIPFPDELEEF
                     KNYCK"
     gene            46559..47572
                     /gene="aroF"
                     /locus_tag="PKI50_01315"
     CDS             46559..47572
                     /gene="aroF"
                     /locus_tag="PKI50_01315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNT02389.1"
                     /translation="MVIVMRENASDEEIEKLRERIETEDLKVSVIKGRDYCVLGIIGD
                     TTGIDISEIEANESVEAVMKIQQRYKLSNRMFHKDNSVIQVGDKTIGGQELAVIAGPC
                     SVESEEQILELAKEVKEYGAGFLRGGAFKPRTSPYSFQGMKLEGLELLKLARKETGLP
                     IVTEIMSVDMIERFVEDVDVIQVGARNMQNFELLKEIGKTTKPILLKRGLSATIEELL
                     MSAEYIMSEGNSNVILCERGIRTFETYTRNTLDISAILAIKKLSHLPVIVDPSHATGL
                     WWMVEPLAKASVVAGADGIMVEVHSDPANAKCDGQQSIKPEKFRAMMKDITLMADSLK
                     KAN"
     gene            47641..48498
                     /locus_tag="PKI50_01320"
     CDS             47641..48498
                     /locus_tag="PKI50_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNT02390.1"
                     /translation="MEEGDFRRNIAVVGLGLIGGSFAMALRKHNAVRIFGIDIDESVL
                     NKAAGCGVIDEGASNAKKILCKSDMVIIALYPREAIEFIKNYAEYFKPGTIVTDTCSI
                     KQPIIEAAKKSLRQDVEFVGGHPMAGNEFQGFEAASRELFFDTNYIITPHESNSENGI
                     IAVEKLAASIGCKCLTRIGAEAHDRLISFTSQLPHIVAISFANLMMREKGISPFTGRS
                     FKDATRVAALNKELWTQILRMNNLNIIDKIEDMEDMLHKLKDAIKEQDESSLQILFDN
                     AIKSKREII"
     gene            48499..49593
                     /gene="aroB"
                     /locus_tag="PKI50_01325"
     CDS             48499..49593
                     /gene="aroB"
                     /locus_tag="PKI50_01325"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNT02391.1"
                     /translation="MVTIEVSIKSSSKKYNIQIGKNIRQELYKYLSERYRGKKAVIVT
                     DDNVNKLYGASLYRGIVEAGMKCRLISFSPGEKSKSLQVLSGIYEDLAGFNADRGDII
                     IAFGGGVAGDLAGFAAATYLRGISYIQLPTTLLAQVDSSVGGKTAIDLSSGKNLAGSF
                     YHPEIVFIDTLFLNTLEDRYLRDGMAEVIKYGCIKDKSLFDRLSDYENLQQCIADMDE
                     LVCTCCSIKRAVVEADELDRGERMLLNFGHTFGHAVEQYYNYEKYTHGEAVAMGMAEI
                     TKRSEEMGLTERGTAKALADILKKYGLPYEIPVANKKDLINVMRRDKKFSGDSIRLVL
                     LNKLGTAFIMKAKSEELESFLDTTGDARED"
     gene            49596..50873
                     /gene="aroA"
                     /locus_tag="PKI50_01330"
     CDS             49596..50873
                     /gene="aroA"
                     /locus_tag="PKI50_01330"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNT02392.1"
                     /translation="MKEVKIYPGSLAGEVFIPASKSISHRAVICAGLSEGISNIYNVG
                     ISQDIEATIEALRSFGTMIEKADASLRIKGSAVPKLKNSGINCHESGSALRFIIPIAA
                     LTGEPVTFYGEGRLVERPLLQYYNIFEKQGIKYETEAGKLPLHINGRLVPGEFQLEGN
                     ISSQFISGLMFALPLLEGDSLITVTTEPESKPYIDLTLKVLKDFSIHIENHGYRTFII
                     KGRQKYKATDYTVPGDFSQAAFWLAAGALGSEVVCKGLDINSMQGDRVMMDIIGEMGG
                     GIFIKAAAVRALPADTRGTVIDASQCPDLVPILAVLGALSKGRTEIVNAGRLRLKESD
                     RLKAVSSELGKLGACIMELHDSLVIDGRKNLRGGRVESWNDHRIAMALAIAATRCEEP
                     VIISNASCVKKSYPDFWVHYKALGGKIDERYMG"
     gene            50854..51942
                     /gene="aroC"
                     /locus_tag="PKI50_01335"
     CDS             50854..51942
                     /gene="aroC"
                     /locus_tag="PKI50_01335"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNT02393.1"
                     /translation="MSGIWGNSLKISIFGESHGKAIGINIDGLKAGILLDLDFIRREM
                     QRRVPGNSEFATPRKEGDEFEILSGFFEGRTTGTPLCAVIHNTDKKSEDYEELKNIMR
                     PSHSDYTGYVKHGGFNDYRGGGHFSGRLTAPLVFAGAICKQLLESRGITIGSHIRSIG
                     EEQDRPFNPVSVDSGTLKKLAGKAFPVLDAKSEALMKEVIARAKEQKNSIGGVVETAV
                     LGVKPGIGEPFFDSVESTLAHLIFSIPGVKGLEFGAGFDISKLMGSEANDEYYMDGEE
                     VKTYSNNNGGILGGITDGMPIIFRTAIKPVASIGRRQRAIDIEKKENVGLEVQGRHDP
                     CIVPRAVPVVEAVTAMALLDLIMVSQGK"
     gene            51965..53077
                     /locus_tag="PKI50_01340"
     CDS             51965..53077
                     /locus_tag="PKI50_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNT02394.1"
                     /translation="MDELEILRQKINETDEKLVKLFIERMALAKKVGEYKSGKNIRIY
                     DPAREKDIVGRFVNRVRVDFDIKYIEQFLENLMFLSRRLQGDIIGRKQEPSKIKNLNT
                     NMAIGYQGVPGCYSYQAGLEYFGEGIEALSYHSFRDVFDALAKGEIQYGILPIENTTS
                     GSINEVYDLLREYEFYIVGEKCLRVEHSLLAVEGAKLSDIKEIYSHRQGLIQCSRYLS
                     QYPEWKQIPYFDTAGSAEYIAKEGLKSKACIAGEKAASVYGLCIICRGIQDNRNNSTR
                     FVIISKEKLDNEKADKISIVFSVPHKPGSLYGILRYFAEGNCNLMKIESRPLEDKAWE
                     YFFYTDFSGNINEEGIRGILADMEKECSYFRLLGNY"
     gene            53095..54393
                     /gene="aroE"
                     /locus_tag="PKI50_01345"
     CDS             53095..54393
                     /gene="aroE"
                     /locus_tag="PKI50_01345"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNT02395.1"
                     /translation="MMNNVFGLIGESLRHSFSPQIHCEIFKMLGIEGEYRLFELQEEE
                     LEPALLKYREQGIKGLNVTVPYKIKIMPYLDVISEEAEKIGAVNTISLANDVLTGYNT
                     DYRGFGMLLARSNIEVKNKTAVILGTGGVSKAAAQYLMDNGVKRITYVSRNPGDGAVG
                     YDETESLKGDIIINCTPCGMYPKADVSPVPYGTFKQFGTAVDLIYNPEKTMFLKQAEE
                     NGLKAVNGLYMLVGQAVAAQEIWNGIGLSRSQVDNIYQHIKKMLYKADTSKNIVLIGL
                     SGCGKTSIGRLLAEKLERKFIDIDDMIEKQEGCTINELFRQGEEHFRNLETKAVLALE
                     REKALVISTGGGVVKRAVNMKSLKKNGIIIFIDRDVCDIMDDIDTSTRPLLAGGTGRL
                     LQLHAERYDLYKEYSDFTVCNDSKAADIIEDIYRKLVEKL"
     gene            54453..55088
                     /gene="nadD"
                     /locus_tag="PKI50_01350"
     CDS             54453..55088
                     /gene="nadD"
                     /locus_tag="PKI50_01350"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNT02396.1"
                     /translation="MYEDAVDNGQSRIGIMGGTFDPIHYGHLAAAEAARIEFGLCKVI
                     FMPVGNPPHKQSQAISDAEHRYRMTALATSSNSGFEVSRLEVDKAGITYTFDTMKELR
                     NIYGEAPAIYFITGADAVLELLTWYKLGELLTLCKFIAVTRPGFDICKLEQKIAEITS
                     KYDGEIICLEVPLLEISSTDIRERIRSGKPVKYLLPEEVEAYIHKNGLYKE"
     gene            55094..55696
                     /gene="yqeK"
                     /locus_tag="PKI50_01355"
     CDS             55094..55696
                     /gene="yqeK"
                     /locus_tag="PKI50_01355"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNT02397.1"
                     /translation="MLDLNSIMEHLEKTISSKRYIHSVNVSKTAKKLAEFYEYDAVKA
                     EIAGLVHDCARDLEKPLQLKCLKEEGIEADELTLNIEELLHGPAAVHICRKVFAIEDE
                     EILSAVRYHTTGKENMSLLEKIIYLSDFIEPSRSFPGVEELRGLAFKNLDKALLLAFN
                     SSILYILSKNEYVHINTILARNHVLKEVGENGMQYHDSSR"
     gene            55747..56724
                     /locus_tag="PKI50_01360"
     CDS             55747..56724
                     /locus_tag="PKI50_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNT02398.1"
                     /translation="MKKYIIIFLASFLVFFAIIGGVYNKYKSNMTDEHFVRTIEENPE
                     LEPEDTKEDVIINTLVMGIDEARSDTMIVVSYNKENQKIALLSIPRDTRVEIPGYGID
                     KINAAAAKKEGTALAMRTVGNLLDVPIHHYVKVTFKGAERIIDILGGVSVNVPPGMDY
                     EDPVQDLYIHLKPGKQVLNGKDAVKFARFRSGYPDQDLGRIRAQQQLIKAFIDKLTSP
                     KMIPKALTIVDAMSKCIKTNLESSDIAHYAINIKDIKMENVSFYTLPGSDGYINRVSY
                     FLHDEAKLKEMIQQIRIDLGVEEPAEEMELLPADNDNAPEGAESGIGLR"
     gene            56822..57154
                     /gene="rsfS"
                     /locus_tag="PKI50_01365"
     CDS             56822..57154
                     /gene="rsfS"
                     /locus_tag="PKI50_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNT02399.1"
                     /translation="MANKAIELLKGKKAKDIRLLDIHELTTISDYFVIAAGSSTTQVQ
                     AMSDELEEKMELAGYEMYHKEGFRNGRWILLDYGNVIVHLFHDEERQFYNLERLWVDA
                     KTVLVDNE"
     gene            57187..57837
                     /locus_tag="PKI50_01370"
     CDS             57187..57837
                     /locus_tag="PKI50_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT02400.1"
                     /translation="MSKDLHPILKGIIPLIDGIANTFGKNCEVVLHDIRNPQSSVIAI
                     ANGHITGRSIGSPMTEYGLATLRSGQFDKPIVNYRKKTRDGRILKSSSLFIKDENGKL
                     IGFLCINYDISELTIARNIINNLANVIEETDFSEEDECFGNTVNEMLSSIVNKALESV
                     GKPVAFISKEEKVNIVQLLDEKGVFLIKGAIDYVAKVLCVSRYTVYNYLDETRVND"
     gene            57859..58242
                     /locus_tag="PKI50_01375"
     CDS             57859..58242
                     /locus_tag="PKI50_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT02401.1"
                     /translation="MTKEIISAEKAPGAIGPYSQAVKYGNMVFTSGAIGVDPATGVFP
                     EGGIQVQAKQCLENLKAVLEASGSSLENVLKTTVFIKDMNDFSKINEIYGQYFTEKHP
                     GRSCVEVARLPKDALIEIEAVAYIG"
     gene            complement(58374..59594)
                     /locus_tag="PKI50_01380"
     CDS             complement(58374..59594)
                     /locus_tag="PKI50_01380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNT02402.1"
                     /translation="MKRLIAYIMILSLMMPFVRVYGDVPEVTAPSAILIDAETGKILY
                     EKDADEKRYPASTTKVMTGLLAVEHGKLDEIVTIGKNPPLVEIGSSQIYLVPGEQLTM
                     EQLLYALMLESANDAAVAIAEHISGSVEEFAKLMNSKARELGAASTNFVNPNGLHNDN
                     HYTTARDLAMIAKYAMTLDKFRDVVKEVKYTIPKTNKQEERNYITNSNKLIWEIYDQF
                     RYEYATGIKTGYTTKAKQCLVGGAKKGDLELISVVMGAEGQNMYSDTVALFEYGFSNF
                     QKVQILKKDQIVTSVPIKEGQEKINLLAAEDFSMVLSESEKENITTEIKTRDGLREPI
                     EKGEVLGEIIVYAEGNEVTKIELLSSVSVEAVKKPILWWLWAIAGFLLYRTVVTIYKV
                     RRRKKYRNVTYIKR"
     gene            59930..60544
                     /locus_tag="PKI50_01385"
     CDS             59930..60544
                     /locus_tag="PKI50_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNT02403.1"
                     /translation="MLYITRTQKSILAFVILVLVIISGTIYIRQQKATEISLAEAVPD
                     QAEAQAETKPAEISVYIYGFVKNPGVVKVLEGTRLDEAIRLVGGAVEEADLEAVNLAY
                     RLADEDAIYIPKKGESPQDTGKAIPGVNTVKSAAVNKPGKVNINTAGEKELDTLKGVG
                     PATAKAIIEYREQKGLFNTIEDIKKVSGIGEAKFNNMKDKITVE"
     gene            61012..62058
                     /gene="selD"
                     /locus_tag="PKI50_01390"
     CDS             61012..62058
                     /gene="selD"
                     /locus_tag="PKI50_01390"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:61063..61065,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNT02404.1"
                     /translation="MDSKNKTRLTQMTKSSGUAAKIGPETLAQVLCQLPKFYDENLIV
                     GIDTSDDAAVYKIDEDKAVILTMDFFTPIVDDPYTFGQIAAANSLSDIYAMGGRPVTA
                     MNIVCFPTCLPIDILKEILKGGADKVKEAGAIVVGGHSVEDDEPKFGLSVMGMVHPGK
                     VTANSGAKPGDMLILTKPLGIGILNTAIKADITDQEAYKKAVDVMSYLNKDACEAMMK
                     VGINGCTDITGFGLLGHGYEMAEASNVSLEIWSDYIPIIKESIDFAKMGIIPAGAYNN
                     EGFIGEHIRFDKSIKQEVRDIMYDPQTSGGLLISVPEQNSEILMEELRKNNKTDFNII
                     GKVKTKGKYSIEVI"
     gene            62127..63575
                     /gene="selA"
                     /locus_tag="PKI50_01395"
     CDS             62127..63575
                     /gene="selA"
                     /locus_tag="PKI50_01395"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNT02405.1"
                     /translation="MIDKKSILSSIPSVDELMKDKELERLTVLYSRTAVVEGIREYLR
                     EYRKHVMSAKKDEELDLANGSDIMKGIIEKIHNMMRNNLVNVVNATGVVLHTNLGRSP
                     LSSRLKDTLWNIASRYSNLEMDLEAGERGSRYSHVEGLICKLTGAEAAMVVNNNAAAV
                     MLVLSTVAKGREVVVSRGQLVEIGGSFRVPDVMKQSGAALVEVGTTNKTHLEDYESAV
                     NENTAALMKVHTSNYRILGFTSQVEPEELVVLGRKLGLPVIEDLGSGLLIDLTEYGLP
                     YEPTVQQAVKAGMDVVTFSGDKMLGGPQTGIIVGKREYIDKMKKNPLTRAMRIDKLTL
                     AALEGTLKLYTDKELALAEITVLRMLTYDNEELGKKARRLGVRLKKTIGDMAAIGIED
                     EFSEVGGGSMPLHKMPTKVVAIKPKTVSLTGLEEKLRAYKTPVIARIVRDRLLLDIRT
                     IMDDEYKVVENAMLWAFTQLSGSKLGEGEGQA"
     gene            63572..65482
                     /gene="selB"
                     /locus_tag="PKI50_01400"
     CDS             63572..65482
                     /gene="selB"
                     /locus_tag="PKI50_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNT02406.1"
                     /translation="MKNVIIGTAGHIDHGKTTLIKALTGRDTDRLKEERERGISIELG
                     FTYFDLPSGRRAGIIDVPGHERFIKNMLAGAGGIDAVILVIAADEGVMPQTREHLNIL
                     SLLQVKKGIIALTKKDMVDQEWLEMIIEQVREETAGTFLEDASIIPVSSISGEGLKEL
                     IAEIDSVTEIVEEKDAHKIFRLPADRAFTITGFGTVVTGTLISGSINEGDRIEIYPRK
                     TETRVRSIQVHEKNVKTAYAGQRVAINIANIKLDEIRRGDILAQPGCMEPTMMLDARL
                     EILKDADKIIDNRDRLRFYQGTSEIMCRVVLLDREELKPGESAFVQLRLEEETACMKG
                     DRFVIRTYSPMLTIGGGTVLEPNPPKRKRFKEEVIDELKIKEQGNPTEVVERYLIQNS
                     DKFPEKKTVLKAAGNMTAELYDKIAAELLEKKLIYEFRLGDETYILHMKYLRDVETKL
                     TELLESYHRRNPLKSGISKEELRTKVFEGMKPRLADMVFGYFEDGGIIRLENQYTALS
                     GFKVQFSKQQEHIRDTILQEYKKNRFNPPRLSELAAAKRLNINQCQEVYIALIEMGEL
                     VKLDEDIAFSKYAYNEAAELLRKYIKDNGSIQLGQFRDLLGTSRKYAMALLDYFDQNK
                     ITKRIGDNRVLF"
     gene            65673..65840
                     /locus_tag="PKI50_01405"
     CDS             65673..65840
                     /locus_tag="PKI50_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNT02407.1"
                     /translation="MADKNLTCKDCGTEFVFTEGEQAFYKEKGFENEPQRCPDCRKAK
                     KAQRNDRDFRR"
     gene            complement(65928..66024)
                     /locus_tag="PKI50_01410"
     tRNA            complement(65928..66024)
                     /locus_tag="PKI50_01410"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65988..65990),aa:Sec,seq:tca)
     gene            complement(66295..66531)
                     /locus_tag="PKI50_01415"
     CDS             complement(66295..66531)
                     /locus_tag="PKI50_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HNT02408.1"
                     /translation="MSGKRTGRRSSKILVPEAEVAMDTLKKSIASEFGIDFNSDYKGN
                     LSAKQCGKVGGEMVRRIIEQMKQEIAQSPGRFRQ"
     gene            complement(66768..67691)
                     /locus_tag="PKI50_01420"
     CDS             complement(66768..67691)
                     /locus_tag="PKI50_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNT02409.1"
                     /translation="MDFNTAVNQILVLFIIMFIGTIAKKSRIIDEGIQNSISVLLMQI
                     ALPSLILSSSNIARTAEVLPNLISILTITSIAYILTILMCMLTVKGLRFDQKTSAVFI
                     SLIVFGNVGFMGYPVVRAFFAETGVFYATASNLVFTAFLWTYGILLFNKQEKIDLKKL
                     LNVGSISTLIAILMFAFRISLPYSLQTAFDLIGKMTTPLSMLLIGVLIAEIDTSKLVS
                     DKRIYLVAAIRLIIIPLATACILRFMGFNTMVISICTLMAAMPSGATNAIFAAQFDTE
                     PLFASVGVFITTLLSILTLPFTVYMLTNFIL"
     gene            67852..68193
                     /locus_tag="PKI50_01425"
     CDS             67852..68193
                     /locus_tag="PKI50_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02410.1"
                     /translation="MVDNDRDDCFLKEIVVLNEILTPLVSSYRLSVGAAEEFNRIALA
                     HRKDVEDAIDRADDLGHLVDDVRRKLKKCMKIYFSELDYKLEHMEEIREKAAMREKLN
                     SKLNKMSEEKK"
     gene            complement(68237..69430)
                     /locus_tag="PKI50_01430"
     CDS             complement(68237..69430)
                     /locus_tag="PKI50_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNT02411.1"
                     /translation="MRTERLYNDDPYLTEFRAEVIETLTYGDKYGIVLDRTAFYPAGG
                     GQPWDTGYLNDEKVTEVIEKDGKLLHIVDNKLLSTEVKGSVDWARRFDFMQQHTGQHI
                     LSACFEKLLTGSTDSFHMGKDIVSIEINIDSFSIDDAERIENMANDIIYSNLPVIARI
                     VSGEELNSLPLRKMPKVTENIRIVEVKETDYSPCGGTHVRTTGEVGMIKIKNWEKCKG
                     GIKFAFVCGNRALKDYGLYNSIIRALCEKLSARDSEIVEAVDKLLFDLRNTEKQLSAS
                     TQELIRIEADNTIKECPVVSGIRLISRVLDNRSINDVKLLAQYLTKVPGTVALLACKN
                     ENAQVIFSRSEDVNMDMNALFKAVLPIIDGKGGGNSRTAQGGGSRVDRLEEFMNNAKN
                     LIISK"
     gene            complement(69521..70240)
                     /locus_tag="PKI50_01435"
     CDS             complement(69521..70240)
                     /locus_tag="PKI50_01435"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013173749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNT02412.1"
                     /translation="MDSSKLSLLASWMKQSKYTVVLTGAGMSTESGIPDFRSPGGWWR
                     NIDPATVASRYALENRYELFRDFYIMRINTLEGCKPHEGHKVLAEWEKRGLVHCIATQ
                     NVDRFHQEAGSRNVYELHGNIRTVRCEDCSNAAELKEFLEKKPCAKCKGKLRPNVVLF
                     GESLPYEALDNATHSISKADLAVVVGTSLQVYPVNQLPELCKGKKAYINLDTGRSSSQ
                     FDLVIEGSARDVLTKAEMLLD"
     gene            70471..70737
                     /locus_tag="PKI50_01440"
     CDS             70471..70737
                     /locus_tag="PKI50_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02413.1"
                     /translation="MNISETLVVPIIVSLTQLAKGLGLPKKFCALAAVVIGVIIGVFF
                     LEPLCIKAGVFKGIVYGLSASGLYSGTKNTIEQVNGKKVKKKKK"
     gene            70805..71188
                     /locus_tag="PKI50_01445"
     CDS             70805..71188
                     /locus_tag="PKI50_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNT02414.1"
                     /translation="MGNNTPKNDQQRRSTGTGVIGFIVRFVVAAIVLMVTAYFVPGFR
                     MRGGFTTAIIAALVIAAADFLIEKLFKFDASPLGRGISGFLVSAAVIYFTQYLVQGME
                     VSLLGAVIGALVIGLVDTVLPTRFM"
     gene            71324..73639
                     /locus_tag="PKI50_01450"
     CDS             71324..73639
                     /locus_tag="PKI50_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNT02415.1"
                     /translation="MASFAFSFFFGILTASIILPDIPIILGGFLLSGAAVLINRKNKP
                     NLKAVFMLLVFLVGMADYSLAYRAESRLDAFDGKTAVYECLITDNPVERGKYLQYPAR
                     SISVQYEGKHYNFSEKVFLKIDADTVFKFGDKLSIRGQCSDIAGIRNPGDFDFKLYYK
                     NKGIAKQIAADRAALLKENSAGVFKAMLYYSKEKVRSIINEALPQEEAAVLTGIITGD
                     KADMDEDMKEAYMKTGLSHILSVSGLHVGFLMMLLTYLLMPFKLDKRLQGAVTLLITV
                     YYVLLIGAPFPSVRAVIMLAVLMAGKAAGREYDLLASVSFAFMIMLVFKPLAIHDTGF
                     MISFAAMYSIALLYPAVYGILRCMPGVIRNPAALSMSVWLGLAPVLAHYFNYISIISI
                     IINIAAIPLSFIITVTGFIGVFAGIVSKTLALYIFSVDYCFINLLSFMIRKAAELPAA
                     GFYIPRLPIYIHALYYGGIGLFIGFCKLAFFRVYMRRLALSYLLAAVIAISVYNLPSE
                     KLRMVFFDVGQGDSCCIITPQKKAVLIDGGGSSRNGDYYYDVGGKITFPALLHQGIWS
                     IDTVIVSHLHDDHMEGLLKVMEGYKIKNIILPRVSAGTDEISGSSGALLDICRNKGIK
                     IHRLGSGDQINLGGGVKIDFLHPGKAAKADENENSLVGVLYYGDFSALFTGDIGKETE
                     GLLQAEALKSVVMKVPHHGSGRSSSEEFLEKVRPRVSVISVGSNNYGHPSPDTLKRLA
                     GIGSLVYRTDGNGGVTIITDGESMKVKTVKQ"
     gene            73702..74784
                     /gene="holA"
                     /locus_tag="PKI50_01455"
     CDS             73702..74784
                     /gene="holA"
                     /locus_tag="PKI50_01455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNT02416.1"
                     /translation="MGYRQLMNDIKNNCIKPVYIIFGNETYLIDKGREALKKAVVTSF
                     PELNYTRLEGEKLEADELSAACRTFPFGTEKRLVEVRNPEILRSKGRGREEDEDEEEN
                     EDEKASAPGKDAQPFIEVLQNLDDTVCLLLLSYGSINKKRKKLLDEVKKHGAVLEFNR
                     VERDELAQWIKKTLGKSGKNIGPRELNHFISSTGYLDKNGSKTLYDLENEIKKLTGFM
                     GNSTEVTIEHIEAIVPRNIENDIFKLINACYEKDTGRSLRIFNDLLLEGETTLGVLAL
                     LSSQVKNMLKVYELYEKKYNRRGISEKLKLHEYTVKLCIQYGSSIKRQALQSAFRKCL
                     DTDLSIKSGGMGDRLAMEMLLAGLFE"
     gene            complement(74851..75114)
                     /gene="rpsT"
                     /locus_tag="PKI50_01460"
     CDS             complement(74851..75114)
                     /gene="rpsT"
                     /locus_tag="PKI50_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNT02417.1"
                     /translation="MANIKSALKRIKVANFKTRRNRIIVSSLKTSIRRFEDSIKNGNM
                     SEAKELYRKVASLIDKAVSKGTLHKNTAARKKSRLSKKIKDIA"
     gene            75314..76327
                     /gene="gpr"
                     /locus_tag="PKI50_01465"
     CDS             75314..76327
                     /gene="gpr"
                     /locus_tag="PKI50_01465"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HNT02418.1"
                     /translation="MKNTLQIFGGNAVAGIRTDLAIEAREFYNKQENRQDIPGVVVDV
                     DKDESYTVTRVKVVEDVGARIIGKPKGNYITIEVPKLRENDRELQEEVGKVLAEEVAR
                     LMSLNDQSVIMVVGLGNWNVTPDALGPRVVEHLLVTRHIKEYVPDQIDEGVRSVCAIA
                     PGVLGITGIETSEIIRGIVDKVNPDLVIAIDALASRKMDRISTTIQIADTGINPGSGV
                     GNNRKELSRESMGVPVIAIGVPTVVDAATMASDTIDMVIDTLINESPESGEFYNMLKS
                     MDKTEKHKLIDEVISPYIGNLMVTPKEIDRLIDDIAIVIANGLNIALHPNVDQDEMNR
                     YLN"
     gene            76437..77639
                     /locus_tag="PKI50_01470"
     CDS             76437..77639
                     /locus_tag="PKI50_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HNT02419.1"
                     /translation="MNKKWLFALFLALAVAASLLFLKWLYSPDPYLSKYNISSTGSAE
                     VFNYDIKKRLKLEEYSNNFYIQLLNTVMTGAKANYEIEYGCPMPGLLESAASSITDGL
                     HTGAGNPITYLNFVFTAFSQYDPSLFSYAETGSSAFPNITDFENAPQGAIYFSEEDEY
                     KAEIPAETPASQIVEEAVAIPGKIALNSKSPQILVYHSHSTESYMPNTAGNYHTQNDK
                     YNVIAVGSIFAKELQDKYKYKVLHDKTKHDKESYAYSYANSLQTIKNSINKYNSIKVI
                     LDLHRDAFDAEKATFAEKTAKKSEYTTSINGKRAARIMLVIAKENPNYAELEKFAVYI
                     KKKMDKLYPGLFYKIDIKSRGKYNLYFSNHSMLVEVGCMLNTIEEAQYSAELFARVMG
                     EVINDLKE"
     gene            77646..77978
                     /locus_tag="PKI50_01475"
     CDS             77646..77978
                     /locus_tag="PKI50_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02420.1"
                     /translation="MSKRVDKKKQRMEALKNKLKKALGIFFCIILLILSVSIADMSAR
                     RMMMCNDDKYAIAVSLQEDSLLRVDIAGEKLMINIEPAVRIFNYIYDNSTRHCDSIVK
                     SIKAKLNE"
     gene            78251..80059
                     /gene="lepA"
                     /locus_tag="PKI50_01480"
     CDS             78251..80059
                     /gene="lepA"
                     /locus_tag="PKI50_01480"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNT02421.1"
                     /translation="MAGDRQKHIRNFCIIAHIDHGKSTLADRLIEKTGVISARDMEEQ
                     VLDNMALERERGITIKLQSVKLEYTAQDGQEYVLNLIDTPGHVDFTYEVSRSLAACEG
                     AVLVVDATQGIEAQTLANVYLALDNNLELYPVINKIDLPSADPEFVKKEIEDIIGLDA
                     SDAALISAKEGLNIEEVLEGIVKYIPHPAGDEDKPLKALIFDSFYDSYKGVIAYIRVV
                     DGVLKPGMNMRMMSNNKEYEVVEAGTLKPWLNPTGLLMAGDVGYVTASIKNVKDVHVG
                     DTITDADNPCKEALPGYRKVTSMVYCGIYPADGADYENLKDALEKLQLNDAALLFEPE
                     TSVALGFGFRCGFLGLLHMEIIQERLEREYNLDLVTTAPSVIYHITKTNGEKLEVSNP
                     TNLPVPQEIEYMEEPIVKATIMTPTEYVGAIMELCQNRRGTFRDMQYMEEKRAILNYD
                     LPLNEIIYDFFDALKSRTKGYASFDYELKGYERSDLVKLDILLNTEAVDALSFIVHKD
                     KAYARGRSMAEKLKEVIPRQMFEIPIQAAIGTKIIARETVKALRKDVLAKCYGGDISR
                     KKKLLEKQKEGKRRMRQVGSVELPQEAFMSVLKLDD"
     gene            80062..81198
                     /gene="hemW"
                     /locus_tag="PKI50_01485"
     CDS             80062..81198
                     /gene="hemW"
                     /locus_tag="PKI50_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNT02422.1"
                     /translation="MGEKMKEVGLYIHIPFCRSKCLYCDFNSYPCREDDAPEYTEALL
                     RELHAYQERYNFVYKTVFIGGGTPTVINCRLIGEIMDKIAPRIKAGAEISMESNPGTV
                     TFDTLKYYRSLGIKRLSIGLQAWQAELLGELGRIHTTEDFLNAYNWARESGFDNINTD
                     LIFALPNQTTDMWKETLDKVVSLGVDHISCYSLILEEGTGLYRLYKEGKLQLPDEELD
                     REMYRIAVDTLGSNGYLQYEISNFARKNMECRHNLVYWRNEEYLGVGAGSHSKLEGKR
                     FWNYKDINRYISLAAEGKLPVEGQEELSADEDMWETIFLALRLNEGLDMAGFNSKYGV
                     DFRTRYGSQLKKLAAQGLVFIESDRLKLTKKGIDLSNSVFVEFA"
     gene            81858..82889
                     /gene="hrcA"
                     /locus_tag="PKI50_01490"
     CDS             81858..82889
                     /gene="hrcA"
                     /locus_tag="PKI50_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNT02423.1"
                     /translation="MDMGNRKRLILQAIIEDYINSAEPVGSRTISKKYLTGTSPATIR
                     NEMSDLEDMGYIEQPHTSAGRIPSDKGYRLYVDKIMKQDKINEAQREMIKREFVESLG
                     EIDRLIKHASKLLSQLTQYTSIVMAPQFRRTSIKQVQLLRIDRAVVLAVIVTNAGIIK
                     NSVLRLKQDIQPDALIRITSMINDKMAGLDVEEIEKFDFQDMLNNSLGHSEIIEQVVP
                     ELIQTLFYSDTSEVYHDGVSNILNLPEYSDINKARDFLNIMEERELLFKVLNDVREDV
                     SVSIGKENKLEQFKECSLITATYKINNKVIGSVGIIGPTRMEYSKAISVVECMTNNLS
                     EILTSILKR"
     gene            82923..83504
                     /gene="grpE"
                     /locus_tag="PKI50_01495"
     CDS             82923..83504
                     /gene="grpE"
                     /locus_tag="PKI50_01495"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNT02424.1"
                     /translation="MKHKKQKEEEKPQVNNTIEENTAEEASAQTGEAVPDQAAETDNK
                     EILELKKQSEEYYNRLLRMQADFENYKKRIAREREDMYNNVLETITAQLLPIVDNMER
                     AVEAFNKDKLDEQYISGVEMVCNQIIDILKKNGVKEIEAMDKEFDPNLHHAVMKVPGE
                     DEDENKIKEVFQKGYILGNKVIRPSLVKVSVKQ"
     gene            83533..85347
                     /gene="dnaK"
                     /locus_tag="PKI50_01500"
     CDS             83533..85347
                     /gene="dnaK"
                     /locus_tag="PKI50_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNT02425.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGGEPVVIPNAEGGRTTPSVVGFSK
                     TGERLAGQVAKRQAVTNPERTVISIKREMGSNFKVNIDDKSYTPQEISAMILTKLKQD
                     AEAYLGEKVTQAVITVPAYFSDSQRQATKDAGKIAGLEVLRIINEPTAASLAYGLDKE
                     ENQKILVFDLGGGTFDVSILELGDGVFEVLATNGNNRLGGDDFDQRVVNYLADEFKKD
                     NGIDLRNDRMAMQRLMEAAEKAKIELSGVLQTNINLPFITADASGPKHLDVTLTRAKF
                     DELTHDLVEMTMEPARKALADAGLTADKLSKVILVGGSIRIPAVQEAVKKLTGKEPYK
                     GINPDECVAIGAAIQAAVLTGEVKDVLLLDVTPLSLGIETLGGVFTKLIDRNTTIPTK
                     KSQIFSTAADGQNSVEIHVLQGEREMAAYNKTLGRFQLSGIPPAPRGVPQIEVTFDID
                     ANGIVHVSAKDLGTGNEQKVTITASTNLSDSDIEAAVKEAEKFAAEDKKRKEEVEVRN
                     NADSLVYQTEKNLKDLGDKLGSEDKARIEAELNNVKEALKGTDTEAIKAAAEELTKVF
                     YDISGKIYQANAGAQGPAGEAPQDDNTVNADYKVEDDK"
     gene            85480..86658
                     /gene="dnaJ"
                     /locus_tag="PKI50_01505"
     CDS             85480..86658
                     /gene="dnaJ"
                     /locus_tag="PKI50_01505"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNT02426.1"
                     /translation="MASKRDYYEVLGIDKSASEEDIKKAYRKLAKKYHPDMNQGDKSA
                     ESKFKELNEAYEVLSDKDKKARYDQFGHAGVDPNGFGGAGGFGGFGGFGGFGGSTGTG
                     GFEDIFDMFFGGTGGFGSTGSAGRKTGPQKGTDLKYELEIEFEEAAFGVKKDIHVTRN
                     ETCNDCKGTGAKAGTSPETCKACGGSGEVRFTQSTIFGRVVNVRTCDECHGEGKVIKE
                     PCPQCKGRGSIRRSRKITIDIPAGVDTGSVMPLRGEGEPGLRGGSNGDLYIYMRVKPH
                     KLFKRDGINLYSEMPISFTQAALGDEIEVNTLEGKMKYTIPEGTQTGTTFRIKNKGIP
                     SLKSKMRGDLYFTVKVNVPKKLSEKQKELLRQYAAISGDDIEDQGKSFFDKVKDAFGK
                     "
     gene            86821..>86978
                     /locus_tag="PKI50_01510"
     CDS             86821..>86978
                     /locus_tag="PKI50_01510"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNT02427.1"
                     /translation="MKYVELQVITTTEASDAVSEILYSEGASGVLIEDPNDFNALNKN
                     EKSWDYIE"
BASE COUNT        27344 a        15202 c        21459 g        22873 t
ORIGIN      
        1 aataatagta gtatgagcat gagcaattta caatatgaaa atattactat ataatagacg
       61 catgacaagg gaaacgagta caaaaggagg ccggaataat gcaggacaac agcaggatag
      121 tatctgcgga cagagataaa ttttacaaga ttatgattac caggctggag ggtttgctct
      181 caagtgaatc tgactggctt gccaatttat caaatgccgc ggcattgctt tatacgaacc
      241 tggaggatat caattgggcg ggtttttatc ttatgaagaa ggaagagctt gtattggggc
      301 cttttcaggg gaaaacagcc tgtacaagaa taaagccggg taaaggggta tgcggtacgg
      361 cagcggcaga aatgaggaca cagcttgtgc cggatgtgca tttgtttccg gggcatatcg
      421 cctgcgacag tgcttcaaac tctgaaatag tggttccttt aatcaaagat ggaacagtat
      481 tcggagtatt ggatattgac agccccataa aaaacaggtt tgacgaactg gatgccaaat
      541 accttcagct ttttgcagat aagcttaata agtatattga ttggagccaa atcagctgag
      601 gaggtaattg aatgaagttc ggcagagaaa gcggaatact tctgcatcct acctcgctgc
      661 cttcgaaatt cggtattgga gaactgggca gcgaagcata cggtttcgcg gattttttgg
      721 ccagaagcag gcagaagctt tggcaaatac tgcccctggg ccccataggc tatggtgaat
      781 ctccgtatca gtgcttttct gcctttgcag gcaatactat gctgataagc attgagaggc
      841 ttattgagga taagcttttg cttcaggagg atatcgggcc actgcctgat tttgatgagg
      901 ggcttgtgga gttcgaaaag gtcagggcat ttaaatctaa cttgttcaga aaggccttta
      961 agagatttga agctgccggc gccggtgaag attatgacag gttcttaagg gagaacgatt
     1021 actggcttaa tgagttttct ttttttatgg ctttgaagga atatttcgga ggagctgcct
     1081 ggaatctctg ggataaggaa atagccttca gggactccaa agcaatgata tattacaggg
     1141 ataagctgtc ccgggaaatt gagtatcaaa gttttctgca atatgttttc ttcagccagt
     1201 ggaggctgct aaaagaatat gtaaacaaac agggtatcag aataattggg gatttgcctc
     1261 ttttcatatc ctatgacagc agtgatgcat ggtctcagag ttatctgttc cagttggacg
     1321 aggagggcaa ccctttaaag acagccggag tgcctccgga ttatttcagc gaaacggggc
     1381 aattttgggg aaacccccat tacagatgga atgagatgga gaaagacgga ttcaaatggt
     1441 ggagagacag gctggatctg ttgtttcaaa tggcggatat aatcaggata gaccatttca
     1501 gaggcttcga aagctactgg gagataccgg caggagaaaa aacggcacaa aagggatgct
     1561 gggtaaaagc tcccggcagg aagcttttcc aaaccttaag agcacatagg aaagatatgc
     1621 atattattgc tgaagatctg ggttttatta ccaaggaagt ggaagaactg aaaaatgagt
     1681 ttggttttcc cggtatgaag atattgcagt ttatcttcaa cggccctttg gaagaagaat
     1741 tgcttcccca taattttgaa gagaattcgg ttgtatatac cggaacccat gacaatgaca
     1801 caacaacagg ctggtacctg aagatgaaga tagcaaaccc caaagcagat tatgagatga
     1861 aggattattt tcatatatcg ggagaaatcg gagagaaagc aatgtgctgg actcttatcg
     1921 aggcagcttt gagcagccct gcaaatacgg caataattcc gatgcaggat ttgctgtgtt
     1981 tgggaagtga ggcacggatg aatattccaa acacaatagg aggcaggaac tggggcttga
     2041 gacttagaaa gggtatgttg acaacagaac tggaggagaa gcttgcaagc ctgtcggaac
     2101 tgtatggcag aaataagaat taataaagat aacaatgcaa aaaagctatg gaaaattacc
     2161 atagcttttt tgcattgttt gccccaaaat gtcgtttagc tgtaatttga cacctagctc
     2221 tcgctaggtg ggtatccgga catggctttc tatcttcctc tgcagattat agacttcaaa
     2281 tgaggcctga tatcaggtca tgtaccttgg attcccgtgt cgtctctgta ggttcaaata
     2341 caagcatatc gaacatccca ggattcggtt gtttatttac tattatatta acataaatca
     2401 ttaacatagt ctatagaatt atctgtcgcg gcttgtattt tttatataga gatagttcaa
     2461 ttaacttggt atatatgaat cggatattga tattatttgt tataaagcgc aattttatgt
     2521 atattaaaat aatatttgtt gacctttaca gtgtaaatta tttatactta ataaaaagat
     2581 attaacaggc ttgtaggggg gatttttttt gaagtataca atcctggatt ttgaggcaat
     2641 gattgaaagc gtcagaaaag acggaaggat acataattat gaaattcaag aattgaaggt
     2701 atatgagaat tatctttatt atttcagaaa atatgttgat cataaatatc atgaatgggt
     2761 ggaatacaat ctgttcccca gggagaatat aggcatatac aagctccagc aatccgccaa
     2821 gcttcaattc aacccatcgg aagtgtacaa atactacgtt gatatggtaa gcgtatccaa
     2881 ggagggccaa ttttggaagg cgaaggattt gtacatagat tttattgtaa agggtgacgg
     2941 caggtactat gtggtggata tagacgagtt taatgatgcc atttgcagga aagaactaaa
     3001 ggaaaatgat gtaaagaatg ctctcaacgg tttgaataat gttcttaagg gatattatga
     3061 aagcaatgat cttgagtgct atatacaaag ccttaaagag aagtacggag gcatggggaa
     3121 gctgcttctg agcaagaaaa cggcttgaca tatattggtt attgagatat tatataatta
     3181 ttattaataa ataataatat aatattataa gggggttatc agtaatggat ttgaaaggaa
     3241 gcaaaactga gaaaaatcta tgggaagctt ttgcaggtga gtcacaggcg aggaacaagt
     3301 atacatattt cgcaagtgct gcaaaaaagg aaggatatga gcaaatatcc ggaatttttc
     3361 aagaaaccgc agataatgaa aaagaacatg caaaattgat attcaaattt ttgtgcggaa
     3421 taggaaacac ggcagagaac cttaagcatg cggcagaagg ggaaaattat gaatggagct
     3481 ccatgtacaa ggaatttgag aagactgcca tagaagaagg ctttaaagat attgcggagt
     3541 tttttggcaa ggtagccctt gttgaaaaag agcatgaaga aagatacctg gctctgttga
     3601 aaaaccttga agaggaaaaa gtattcaaac gtgaagagca gacaacttgg agatgcagga
     3661 actgcggata tattcacaca ggagccgaag ctcccgaaat ttgcccaacc tgcaagcacc
     3721 caagagcata cttcgaaata gcagccttta attattaata gtaaactaaa cccctgtggt
     3781 atagaccgca ggggttttaa ttcatatacc tttcatggat ttttcatata cttcaagggt
     3841 ctctgcagca gttttaaacc atgagtattc tttagccttc tcaatgccca tcaggctgta
     3901 tctatgccat tcatccccat cggtaagcag tgtgagcatt gagtcggata tatcatccgc
     3961 agaatatgga tctgtaagaa ggcatgcatc tccgacaatt tcaggtattg aagacacatt
     4021 tgaggttatg acaggtatac cgcattgcat tgcttccagt ggaggcaggc caaagccttc
     4081 gtacagagag gggtaagcaa ataattctgc tccgttgtaa aagtaaggca gatctgcagt
     4141 ggggacaaaa ttcaggaatt ttacataatc gcacagctcc agttcttcgg taagcttcca
     4201 aagagctttg aaggatctgg aatgctctcc gagtataaga aggttgataa cctcctcttt
     4261 ctcaattatt actttcttaa aggcccttat caatctggca atattcttcc tttggctgaa
     4321 gcctcccaga tagagaacat acttcttatc cacaccatac ttgttgaaaa taactttttt
     4381 tgcattgtcc ctatccaagg gcctaaaaat agggtctgcc gccaaatgag ttaccgttat
     4441 tttgtcatct tcaattccga agtatttttg aatatccgat ttggagtggc gagacacagt
     4501 aattatgtgt gatatatcat ccaaaatgta aggggtatgc ttcaatgtgt agtccaggtg
     4561 cggcctgtct acggtttcag gcataatata aggaataagg tcgtggagag ttattacttt
     4621 tacgcccttg aagcaattgg gaagccctat accattgacg gtattgtgga aaacatcaat
     4681 gggataattg acatcccatt taagttcgta tacatttcgc caaaaatcaa ggcgcctttc
     4741 accaatcaga aggttttttg gtatatcatt gatttcaaaa taatcagttc cattcttaga
     4801 tgggtaaaac aaaaatatat tctttaactg tttaagctta ttcaggtttt ccagcaggtt
     4861 attggtatag gtggccaagc cggagccgat atgccaggtt attactcttc cgtcaatacc
     4921 aaggttcatg ttttttagcc tcccgaaata cgcatattat tgtatgggaa agtatacttt
     4981 cctatataat ggggatgcaa gaaaaggtcc ggtgtgagac gcctttttta atcataatat
     5041 gtgagtggga aaagaatgtg atataattgc gttgattaaa gttgatttta gggggaaacg
     5101 catgaataat tacaatgaaa catgaaaccc ggtttcaaag gaatacaaag aggttcatga
     5161 ggaaccgtat atatcagata taagctgtac tgtactgcag cccggtatgg tattcagaat
     5221 agaagacctt gtaatggtaa ctgagaatgg agtagaagtg ctgaacaagt tcacgaagga
     5281 attgattgag atatgaataa agcctgttgc atccgcaaca ggcttttatt atttactaag
     5341 ctgatacgtt ttcatttttt gattttggcc ctttatactc cggagccgac gggtcatctt
     5401 ccagcttcat cattgtgatt ccggtgaaac cgaatacaat ggagactatc gggttaacaa
     5461 gattcaggaa gcagtatggt atatactgaa ttgttggtac tccaagatat gttgacattg
     5521 ttgcaccgca ggtattccaa ggaacgagag gtgaagtaag tgtccctgcg tcctcaaggc
     5581 atcttgaaag gtttcttgga gcaaggcctc tcttgcggta ttcatccttg tacattttgc
     5641 ccggaagcac caaagccaaa tattggtcac ctgcaagtac gtttgtgaag atacttgtga
     5701 ttatagttgc cgtaaccagt gcccctgtgc tctttgcata tcccaatatt accttgccta
     5761 ttgtctccag catgcctgtt ttctcaagta caccgccgaa agtaagtgca gccagtataa
     5821 gtgagatagt ccacatcata ctttgatatc cgcctcttgt aagaagctcg tctattattg
     5881 catttcctgt ttccgatact acgccatact gcatagcatc cataacacct gcaaagcttg
     5941 ccccctggaa tatcattgct gccaatccac ccagaagtgt acctatgaag agaccgggta
     6001 tggcaggaac ctttctggca accattacaa taacaatgag aggaaccagg agaagtaacg
     6061 gattgataac gaagttatcg ctcataaggg caagtatttc attaatggct gatatatcaa
     6121 gctctttgcc agcatagccg gcaccaagaa tgccaaaacc tataagagct attatcaggc
     6181 tgacgccagt agtataaacc atatgtctta catggtcgaa aaggtttgct cccgccattg
     6241 caggtgcaag atttgtcgta tcagacagag gagacatttt gtctccgaaa taagcaccgg
     6301 atataatagc acctgctgct gcacccggat taacaccgag acctgcagca actcccataa
     6361 gagcaatacc gactgtacct gcagttgtcc atgagcttcc tgttgcaagg gataccacac
     6421 agcagagcaa acatgctgca ataaggaaaa agctaggaga aagtatctgc aatccataat
     6481 aaataagagt aggcacaata cctccaagta tccaggtacc gattatcata cctatgatag
     6541 caagaattag gatagcctgc attgtatcgg atattgtcga tacgatacca tcttcaactt
     6601 ctttccaagt gtatccaagt cgcgtgatag caacaataga agctataagt gcgccgagga
     6661 ttaacggaac atgcacatcc tggcctacgc caaattgagg cagcactgca tacataagga
     6721 atcctattgt aataacaacc ggtattaatg cttcaaacaa tgtcgcttct ctctttgtct
     6781 ttgtagacat taacattacc ccttcctaaa ttttttttat ttcactgctt tgaggcgtgt
     6841 ttaaacagat atattaaact taaaataaat ttagaaaaat ttcgttttgc tagtttaaac
     6901 ctaccctatg cagttgaaac cccaggaaca ttataaaagg ctcaaaaaag ccttatttgt
     6961 atatttaaca ttatctcaaa aaaaagggat tgtgtaaata cataaatttt ctaaaagttc
     7021 ggcaaattta accattagag ccgctggaac gcataaatat accaaaatat accgctgcat
     7081 cagtgataat tcattccatg cctaaatggg caaacgtatt ttatgaataa atctgtataa
     7141 aatagtaatt atatacagta tatttacaaa aattttttta aggaaattga taaaatacat
     7201 aaacagcgga atttgatgtg tcgtactttt aaaaaacaga atatttagaa gttttcggcg
     7261 atattgagat tgaaaaatac tttaagttat agtatcttta tagtaactcc acaataaaaa
     7321 ataatagggg gtaattatta tggttaagtt tattctgggc gtaaaaggct caggcaaaac
     7381 aaaaagaatg attgaactgg ctaatgaatc aggcagaaca gcaaagggta atgttgtata
     7441 tatcgatagg gacagaaaca gaattcatga ccttaaccgg agaataagac tgattgaaac
     7501 cggagatttc cagcttgaga atcttaaatc attctatggt tttatctgcg ggataatatc
     7561 acaggatttt gacatagaga acatatacat tgacgggcag aagatcgtaa gcaatgcgca
     7621 ggatgattgc cttgaaggct tcataaagaa tcttgaaata cttcatgaaa aattcggcgt
     7681 ggattttacg attagctgca gcagggctat agaagggata ccggaattcc tcaagagcta
     7741 cctgatatga attaacttgg aaaacaatca aattcagata caaagatggg gacatcgcat
     7801 aactgtttaa catgcagtta acgatatccc ccttttattt ggaggaggac acggggacac
     7861 ggggacgggg acatggggac gtttcccctg tcccacttcc agtgggacac gcgaaacgtc
     7921 cccatgtccc gaaacgtccc catgtcctct ttctaatgac tcttgcctta tgatataata
     7981 ttaaagtaca atgccttaaa gatattcaag tcttgctata ggagtatata tgtttggata
     8041 tataaagcct tcggtacagg agcttaaaat aaaggaatac gaactattca ggggctatta
     8101 ctgcggcttg tgcaaatcaa tgggcagaag ctttggagtg ctctcaagat ttgcactgaa
     8161 ttatgacagc gtttttcttg gattgctgct ggcttctgtt cacggcgaaa aaccggtgct
     8221 taagaaagaa agctgtattg ccaatccggt gaaaagaaaa tggatagtaa aagaaagtcc
     8281 gggtatcgat ttcgcggcag acattaatgt gctgcttacg tattataaat tgaaggatga
     8341 tataagggat gaaggaggat ggctcccgac gtttttaaaa ctggtttttc ataagggcta
     8401 taaaagagct gctttgacaa ataaggacat tgatagaatc atagcttcat ctgtttcttc
     8461 acagatatcc ttggagaaga agggatgcag ttcgacggat gaagcggcag aaccctttgc
     8521 gaatatgatg atgcagctgc ttgcgtcagg atacagcggt gatgacaaat cagtgccgag
     8581 gatactggga tggataggct acaatcttgg gaaatggatt tatattattg atgcctttga
     8641 tgatatggaa agagatatca agagcggaag ctataaccct cttctcagac aatacaaata
     8701 tgaaaatcag gatataaaag attttaaagc ttccattgct gaagaagtca aggtcggcct
     8761 tcttcaggca ttgtcacagg ccacgggttc tttggagctg ttgaagctgg ataataaagg
     8821 cataataagc aacattgcgt atgagggttt atacggaaaa acagaaagag tattgaattg
     8881 cattgcaggg gaaaaaagga gctgtgtgag agatgaggaa tccatacgag gtattgggta
     8941 ttagagaagg tacaagtgtt gaaggaataa aaagggcata taaggaattg gtcaggaaat
     9001 accatcccga ccagtatcag aacaatcccc tctctgatct tgctgaggaa aagcttaaag
     9061 agataaatga ggcttacgat tatcttatga agaaagcgga atccgaggaa aggaacagca
     9121 gctacagaca gcaggaggag ccaaggtacg ggaacaatcg ggagaatggg gacagaggct
     9181 tgtatgatca ggcaaggata tatgtgaata tgggcaatac tgcagctgcg gaggaaatat
     9241 tgaatagaat aagcttcaga ggtggagaat ggtattacct cagaggcctt attttcatgc
     9301 gccggggttg gtataatgag gctgtcacat gcttgaagac tgccgtggat atggaaccct
     9361 ccaattatga attcagggat gcattgaaca gtatcatgaa tgccaacaga cagtatcagt
     9421 ctactgtata tgaaagaagg ggctatactt ccggaccgga tctatgcact ttgtgtcagt
     9481 gcatgatatg taccgattgc tgctgtgaat gcatgggcgg cgacttcatc agatgctgtt
     9541 agaatatgcg cactggcatg cgcatacaag agatatgagt cactagatgc gagatttggg
     9601 cggggttcga aattattaag aatgggtaac gcgggggagt ggcgttatga gcagaaaaat
     9661 ggcctttaaa ggcatatttg caggtatagc agtggtgttc ctgtatttaa gcactataat
     9721 gcctacgggt aaactgacgt tatatttttt ggcgtcattg ccggtggcaa ttactgttgc
     9781 agaattggga gcgggagcgg ggattgctgt ttatttcgct gcttcccttc taaatatact
     9841 gattacaggt aatattacag gtgcagtacc ctttgtattt tttttcggcc attatcccgt
     9901 tttcaagtac tttatagaaa agggaagaaa ggctgcagtt gaattgctgc tcaagcttac
     9961 tgtttttaac atgtcaatct tcctatggta tttatttttc aagaacctgt tcataacggc
    10021 gcttccggct aagttcacgg gaggaagcat actgacggca gtttttatca cagcgatgca
    10081 agcggtattc ctggtatatg attatgtgtt ttccagactt ctcttttatt ataaatcaaa
    10141 gctgagccta ttcaggagat agcacacaaa tactgatgta agatgcatta ttgctaaagg
    10201 ttggactttg aaaggatata aatggtggga tatgaacagc agcataagaa taataagaaa
    10261 aagcgagggc aaagtcagta catggtcggg aggcacgacg acacaattgt atatatatcc
    10321 tgggaatgca gtatatgcag atcgtaattt caggtggaga ttgagttccg caaaagttgc
    10381 ggctgatgaa tcaacattta ctcccttgcc tggaatctca aggatattga tgatcattga
    10441 aggagaagtt gaaattgaac atcaaggcca tcaccgtact gttttaaaag cctttgaaaa
    10501 agacagcttc agcggggatt ggacaaccat atgcaaagga aaagccgcgg attacaacct
    10561 tatgacagat aagagctgca gcgataagaa aatcaatatc atcagaacaa ccatatatta
    10621 ttaagagcct tattgctgca gctatggtat aataaggctt ttctccggat atgacgagaa
    10681 tgccggcggt atgatataat catattagaa atctgataac cgataaataa aagtaaagca
    10741 ataaaatagg aggggtataa tggataaacg tgaagaggct ctggaaaagg taatggttta
    10801 cttcaacggc ggctataact gctgcgaatc ggttgtaatg gctgtatgtg attatttggg
    10861 tgcggacaag gatatacccc aaaaaatagc aactcctttc ggggcaggaa tgagcaaaaa
    10921 tggaagcaac tgcggagcgt tgaatgctgc ttttatctgc atgggcctgg tcggcggcag
    10981 aagatccagt gaagacagca aggatacatc attcttaccg gcagacagga tattcgggaa
    11041 attcaaggag aagtacggat caaccttttg ttatgatatt acaggcttgg atatgaaaaa
    11101 aaccgcagaa acagaagagg aaaaggcgaa aaggcacaat gaactttgcg ggcccctggt
    11161 attgcaggtg acgcagtgga tattggagga actaaatcct taatagaagc tgagtgtaat
    11221 ataaagtggg atattgctaa ctacttatca ggtagtttgc aatatccctt ttcatcaata
    11281 cccgcgtttc ttgaaaaatg taagaaaatg aagtatactg gatggccgca tttttttttg
    11341 ctacatcctg ccgctgcagt tcaatacatt cctcatcttg tgcttaacca tgttctttat
    11401 ggcttctctt gccggcttca gatattgtct cgggtcaaag tctcccgggt tctcggcaaa
    11461 atgctttctt attgaagcgg tcatcgcaag tctcaggtcg gtatctatgt ttattttgca
    11521 tacgcctagt tttcctgccc tcagcagcat atcctccggt acaccctgtg ctcctgctac
    11581 gttgcctcca tatttgttgc acagctcgac gaactcggga agcactgtgg aagcaccgtg
    11641 aagaaccaag gggaaacccg gcagaagctt agttattttc tcgagccttt caaaatccag
    11701 cttaggttct cctttgaact tatatgctcc gtgactggtg cctattgcta ttgcaagaga
    11761 gtcaactcct gttctctcaa caaattcaac agcctggtcg ggatcggtat atgtagcatc
    11821 cttggcgctt acattgacag tatcctcaac ccctgccagt ttgcccagtt ctgcctctac
    11881 cgctattcct ctctcatggg catattcaac aactcgtttt gtgactgcaa tattctcttc
    11941 aaagggatgc ttggagccgt caatcataac ggatgtaaag cctccgtcta tgcaagactt
    12001 gcatatttca aagtcttcac catgatccag gtgcaggcag attgggaggc cggtgtcttc
    12061 taccgccgct tcaaccagct tcataagata aacatgtttt gcatactttc ttgcacctgc
    12121 ggaaacctgc agtatcagtg gtgcgttttc ttccttggcg gcatcgacaa tcccctgtat
    12181 tatttccata ttgttcacat tgaacgcccc tactgcatat tggctctcat atgctttcct
    12241 gaacatttct tttgatgtta ccaatggcat gataattcct ccttttgcta atcgtattcc
    12301 acattaatat agtataccac aaaacctgct gcttttatgg cgtcagcttg agaaatactt
    12361 agagcatttc tactatcttc agttccggaa ccatgctgag atcaattatt gtttcctttg
    12421 attttatgag aggccgggaa atcctgtttg tgttaatata tacgatctcg ccgatggagc
    12481 tgtcgctcag cctgactttt gcgcctatat aataacttga tatatgtttg aggaaagtaa
    12541 gcattatacc ggcatcacac tttgtaagat attcactttc atacatttcc agtacgtcaa
    12601 aaggtgtctg gcgtttttta taaatccggt ttgaggtcat ggcatcaaat gtatcaacaa
    12661 cggccactat ctttgcatag taataaattc tttccgattt aaatccaaag ggataaccgg
    12721 agccatcctc cctttcatga tgcatcaata ctcctaaagc aacatcctta ctgatttctt
    12781 tattgtcctc cagcatctta tagccaataa gagggtgctg ctttattttg tcaaattctt
    12841 cttcagtaag tgtatcaggt ttattgagta tttcgggagg tatttttgat ttcccgatgt
    12901 catgcaatat cccgcaataa gaaagctgct caatgtgctt atgccccaag ttcagccagg
    12961 agcccagcag ggagcataaa agtgatacat tcagactgtg ggcgtaggta tattcactaa
    13021 ttgatctgac cttactgagg catgagacaa gatcgtgtat cgagtcaaaa ttactgctga
    13081 tgtttcttga cacttcgctt acccgttcca tttccaatgt ttttccgcag cttatatccc
    13141 agattatttc tttaaattcc tcaagattgt tgttgtattg ggcctcaatg acagcgtgct
    13201 tcttttcttt gaattcataa tccttgtaaa tcttgacaaa attcaaaccc agcttcatca
    13261 atttgcttat ggagtattca tccaatattg agttctggta aagtatcact gcaccatagt
    13321 cgtttatgat gtcagaagcc aataccatcc ccggcttaca atcagaagtc ctcattattc
    13381 ttatttctgc cattatatca cctgcctcaa gtaaattggt tagtagaata ttcctattaa
    13441 ttaaattata tattaaatca cgataatgta aagcaattag tttaaatcat atggaaacgt
    13501 ctggaattag cttattgcaa tgctgtagta ataattaaaa gaggaattta ttatgttttg
    13561 tagaatatat ttcagtaatc aaaaagatgt ataaatcaaa gaaagtgttt tgcgatattt
    13621 gaatttgaca gaagatgttt gctgcgaaac atatgatatg cgggtgaaaa aatgcttact
    13681 cttaataata aattagataa tctaatattc agggacatta ccaaggagga tctgaaggct
    13741 gtccttaatc tgtataacca gaatgatcag aacatatatg caacaggcat agacagaaga
    13801 atgtccctga gggatataaa ggaaaagtat ctggaagtgc ttgtcaacag ccatgaattc
    13861 tttaccggta tttttcttga gaaagaagat gccttgcagt tggttggtgt aataaaagga
    13921 aggattgatt atgaaaacag cgaggaagca tggatatctt caattcttgt ggataacagg
    13981 tatcagaggc tgggaattgg gaccaaggct gctgaagcgt tgataaacat gctgaataag
    14041 agctatgatg taaagaggtt ctatatagga ataattgccc gtaacagtgt tgggaaatgt
    14101 ttttggcaga agcttgagtt cagctacttc aggactatcg aacagtatat caagctcaat
    14161 gccctcgcag aggattttat aataatgaaa agggaagtgc tgtaaaggga caggagaaac
    14221 gtccccgtgt cccgaaacgt cccccccatg tccctttatc ttttaaggct tgacattact
    14281 tttctcacat agttctgagt ctcttcataa ggcggtatcc cgccgtactt cttaacggca
    14341 ttggggcctg cattgtatgc tgcaagagca agctccagat taccgccaaa ctctgtcagc
    14401 atatccttaa ggtatctgac gcctccttct atattttccc gcgggtcaaa ggcgttatgt
    14461 actcccaggg cttctgcggt ttcaggcata agctgcatca ggcccatcgc ccctgtcctt
    14521 gatactgcag tcgggtcaaa gccggattct gctttcataa cggcggttat tatattttcg
    14581 ttgatattat attttttcgc agcagcttta attaaatggc tgtaatctcc gccataatta
    14641 ttttgaacat cggattgctc attgatctcc ttgttaagta cgtcctcaaa gcttggaggg
    14701 ttgtccttta ttatcttgac atagggtgga agctgatttt gtatttctaa cagcttttgt
    14761 cgaagaatgc cggataaaat ggaagacaat ccaatcacct cctgttggtt ctattttagt
    14821 acagattaaa gggcatatca ataaaaagaa gcaacggaaa gactttgtta attaattgtc
    14881 gattttatgc tttttatatt gtataattaa atatatattg taagcatacc accatagatg
    14941 gtgctctgaa ggaggtttat atatgaaaat atgtgaggtt aaagaagtat tgaacgcaga
    15001 attgctgtgc ggagatgaac tgctggagaa cgaggtttca tatgctttcg gatctgatct
    15061 tatgagcgac gtgctagctt ttgtaaaggg caaaaccctg ctccttaccg gactgaccaa
    15121 ccagcaggtg gtaaggactg cggaaatggc ggatctaagc gcaatagttt ttgtcagggg
    15181 gaaaaaaccc ggtgaagata tagtccagtt ggctactgag aaggacattg cgctgctgtt
    15241 gaccagggat accatgtaca cagcctcggg caagctttac agcaacgggc tggaaggagt
    15301 aagtattgat tgattgattg attgatcgga ttttggcttt ctatttggag gaattattta
    15361 atggaagata ttaagtacgt atttgatgtt aaaaaagatg attttgtcag agcgggagaa
    15421 acatcaagca aagtaaagaa aattttgagg gaattgggta tcgactcaag tattataaga
    15481 aagatttcca ttgccgctta cgaagcggaa atgaatattg ttatacattc taccggggga
    15541 cagattgtgt tggagattac cccggggatt atcaggataa cagcaaatga cagggggccg
    15601 ggcatagctg acgtagaact ggcaatgaag gaaggttatt caacggctac cgataaggta
    15661 agggaaatgg gttttggagc cggtatgggt ttgcctaata tgaaaagatg ctcagacaga
    15721 ttatatgtaa cttcttcaga aggaacaggt actcttattg ttattgaagt agatttgaaa
    15781 gcaggtgagc aaggtgaata aaatgaaaga gaagtacttt cattcggttg tgcttgatgc
    15841 agatagatgc gtcggatgta caagctgttt gaaggtatgt ccgacagagg ctattaggct
    15901 gaaagaaggg aaagcctgta taataggtga aaaatgcatt gactgcggtg aatgcataag
    15961 aatatgtccc aatcatgcga agaatgctgt cacagacagt aaagatatat taaagaaata
    16021 taaatataca attgctatac cgtccctggt gctgtacggg caattccgag gcaatgtcgg
    16081 aatcaatgaa atgcttacgg ctgtaaggaa gataggtttt gatgaaatat ttgaggcatc
    16141 tgccgggaca gagataataa ctaatgtaat aaaaaaccac attctcaaga atcctgagaa
    16201 aaaacgtccc ataataaatt cttcttgtcc tgctgccctc aggcttatac agatcagatt
    16261 ccctgaactg ctgcctaata ttgcggatat agaaacaccg gttgagatag cggcacgcct
    16321 tgcaaaggct gaggctgtca gaaggacagg tttttccatg gaggagatag gggtaatcta
    16381 tatttctccc tgcacagcca ggatgacaag tgtaaaacac cccataggca taaaaaagtc
    16441 ctacatcgat ggtgtactgt caatgaagga tgtatatggc atgatgctga gaaatttagg
    16501 cagcaataca gaggatctga ccagaagtac aaaggaatct ttgttatggc ctataatagg
    16561 aggacaggga aagcctttgg gcataaaaaa ctaccttgct gtagacggaa tacatgaggt
    16621 tattaaaatt ctggaagaaa ttgaaatgga caagcttgaa ggattggagt tttttgaggg
    16681 aatggcctgt gtcggaggtt gtgtgggagg gcctcttaca atagagaacc cttttatagc
    16741 caggagacgc atgaaaaccc ttgcaaaagg gcttccggat tcaaatccaa gtgatgaaga
    16801 gataaaggga caaatagaaa tgtataagag cggattcatc cgattgactg aagcgattga
    16861 gcccaaagcc gtaatgcgtc tggatgatga tatctgcaaa tccattaaga agatggaaaa
    16921 gataaaggaa atgctgaagg acttgccggg aatagactgc ggtgtctgcg gggctccaac
    16981 ctgcaaggca tttgcagagg acatagtgaa gggtgaaaac cggaatgcag tttgcatagt
    17041 caaaaccatg aaaagcttta ataaagaaaa accgcaatca tagggagggt taacatgtta
    17101 gtaagagatg ccggagaaaa aataggattg aagctgttgg caggagaggc cggaaccagc
    17161 agggagatta cgggagtgta tatatgtgat cttttaagct gggttatggc ccatgccggt
    17221 aaaggggacg catggataac cgtgcagaca catgcaaata ttgtcgcagt ggcagctttg
    17281 cttgaactgg gctgtatcat tatacccgaa ggcgtggagg ttgaggagga tactctccga
    17341 aaagcggagg atgagggagt acctgtgtac cagtcgggtt taagcgcata tgggattgca
    17401 aaagaactat acaggatggg aattgagtaa tatggaattt gcggtggatt ttcacataca
    17461 ctccgcatta tcaccgtgcg gtgatgagga tatgacacca tgcaacatag taaatatggc
    17521 cttgttgaaa gggctcgata tcatagcggt tacagatcac aattcctgcg gcaatcttcc
    17581 tgcggttatg gaagcagcaa aggagaacgg attgatggtt attccgggaa tggaggttca
    17641 gacaaaagag gaagttcatt tattgtgcct tttcaagaga atggaaggag caatgaaatt
    17701 cgcggagatt gtttataatt ccttaccgga tatagagaac aacgaagaaa tttttggacg
    17761 ccagctgatt tttaacagtt cagatgaaat aatagggaaa gagcataagc tgcttctgtc
    17821 ttccacaata ctctcagtca atgatgtgtt catattgacc agagggcttg gaggggtgtg
    17881 tatacccgct catgtggaca gacagggctt cagtatcatt accaatcttg gtttcattcc
    17941 tcctgacctt aaggtcaaaa cagtggaaat atcaaaaaga tgcactccgg atgcagcttt
    18001 taagaagtat ccttttctta gcaggttcaa ttatataata tcctctgacg ctcattatct
    18061 tgaagatata agcgaaaggg atttttttat tgagctggat tgcctaaccg tcagtgagct
    18121 tttcaacaag ctggacccca aataataaaa accttaaata aagaatactc caaaccaatt
    18181 attatatacg gttttagaaa aaaataaacg ggaaaatgaa aaatattata atacttttaa
    18241 aaataatttt ttatttttgt taaaaaaata tctaatatgt gatacaatat tgtaggacgt
    18301 aattgacaaa tttatatcaa taaatttttt cgtgagaaac aacaacctaa attttattga
    18361 atccgtgcat tcaatataag ctgttactat ttccaattat aaatatttga aggagggaac
    18421 tctttaatgt ctgacgcagc aatgaaggaa aacatggaga aattggagga ctttatacag
    18481 aagcataaga atcagaaggg tgctttaata ccaattcttc atgaagcaca ggaattattc
    18541 ggatatattc ctgaggaagt acagaagagg atagcggaag tattacacat tccggcagct
    18601 gaaatattcg gcgtagctac tttttattca aggttcacct tgactccgag aggtaagcac
    18661 acaataagcg tatgtctcgg aacagcctgc tatgttaaga actccaaagg agtacttgaa
    18721 agacttgaag aagagctgaa cgtgaaaacc ggaggtacga cagcggatgg aaagtttact
    18781 cttgaggcaa ccagatgcct gggctgctgt ggccttgctc cggtaatgat gattggtgag
    18841 gatgtatacg gcaaattagt tccggatgat atcccggata ttgtaaagaa gtattaatat
    18901 caggatgaag gatttatcac tgcatatcct tgatttgtcc cagaattcga tttcagcggg
    18961 agccggcctt gtggagatat caatagaaga agatacaaaa gaggatcgac ttactatctg
    19021 tataaatgac aatggtaagg gaatggataa ggattttctg gaaaaggtaa tggatccctt
    19081 tgtaactaca aggacaagca gaagggtagg attgggaatt cctcttatgc tggcggcatg
    19141 cagtagatgt gaaggggatc ttgtcgtaga atcggaaaaa aatgtgggaa cacggcttaa
    19201 agctgtattc aaacatagcc atatagacag ggctcctatg ggagatatgg ctgaaaccat
    19261 gttgagttta attcttgcag gatctgatgt taatagaaat gttgattttg tatacaggca
    19321 cattattgac ggaagggttt tttgcctgga tacaagggag atcaggactg ctttgggtaa
    19381 tgatattaac ttgggggagc ccgaggttct gatgtggatt aaggattata ttaatgaagg
    19441 tttaaagaat atacgcggag gtgtgtaaga tgaaaacaat tcaggaattg gaagaaatca
    19501 gaaagaaaac cctggatacg atcaatatca gaaaagatag atcaggcacc agaatagttg
    19561 tcggtatggc aacctgcggt attgctgcag gagcaaggcc ggtacttcta acaataatgg
    19621 acgaaataaa aaaacatgat ctgtcggatg tggtagttgc ccagacaggc tgtataggca
    19681 tgtgcaggct tgagcctatt gtagaagtat ataaacccgg cgaagagaaa gttacttatg
    19741 ttaaggtaaa tccgtcaaag gctattagaa tagtcaatga acatattatg aagggcaagg
    19801 ttgttgaggg atatacaatc ggtgctgaaa gtaaataaag gaaggcttga gggacagaaa
    19861 ggaatatagt aatctgtagg aggtaatatt aatggagatg tatcgttctc atgtaatgat
    19921 ttgcagaggt actggctgta cttcctcaaa ttctgaacag atagcaaaga actttgaaga
    19981 agaaataaag aagcatggtc ttgaaaaaga agttgttgtg gaaagaacag gctgtttcgg
    20041 gctttgtgca ctgggcccgg tagttattat ttatccggaa gcttctttct acagtatggt
    20101 taagcctgaa gatgtagagg aaattgtaaa ggagcatctg gtaaagggca gaattgtgaa
    20161 gaggctgctt tatgcagagt cagttgaaga aaaccggctg aaagcccttg aagaagttga
    20221 ttttttcaag aagcaggtta gggttgcatt aaggaactgc ggtataatta atccggagaa
    20281 tatagatgaa tatatagctt ttgacggtta caaggctctt gaaaaggtac ttaccaaaat
    20341 gacaccggca gaagtaatag atacagttaa gagatccggg ctcagaggaa gaggcggcgc
    20401 cggcttcccc accggaatga agtgggaatt tgcatcaaaa tcagtaaatg atcagaaata
    20461 tgttctttgt aatgctgacg aaggagaccc gggtgcgttc atggacagga gcgttttgga
    20521 aggagatcct cactcaatac ttgaagccat gactattgcc ggttatgcta ttggtgccag
    20581 ccaagggtat atttactgca gggcggaata tccgatagct gttaaacgtc tcggaatagc
    20641 aataggccag gcaaggctgt acggcctgct tggagataat ataatgggaa caggttttaa
    20701 atttgatatt gaactgaggc ttggagcagg agcctttgta tgcggcgaag aaacagctct
    20761 tatagcttct gttgaaggac agagaggtat gccgaggcca aggcctccgt tccctgcaaa
    20821 taaaggattg tttgcaaagc ctacaatact taacaatgtt gaaacctatg caaacatacc
    20881 tgtaatattc aacaaagggc ctgaatggtt tgccagcatg gggacggaaa aatccaaggg
    20941 cacaaaggta tttgcacttg gcggcaagat aagcaataca ggtcttgttg aaatacctat
    21001 gggaacgtca ttgagagaag taatatatga aataggcgga ggctgcccca acggaaagaa
    21061 attcaaagct gttcagacag gcggcccttc aggcggctgc attcctgaag aacaccttga
    21121 ctcacctatt gattatgaaa gccttgctgc attaggttct attataggtt ccggcggtat
    21181 gattgtaatg gatgaagaca attgtatggt tgacatagca aggttcttca tggacttcat
    21241 agttgatgaa tcctgcggaa agtgcacgcc gtgccgtgaa ggcaccaaga gaatgctgga
    21301 aattctcgaa agaataaccg aaggcaaagg tgaagaaggc gatttagata agcttgaaag
    21361 tcttgcaaag aatataaaga atgcggctct ctgcggattg ggacagacag ctccaaaccc
    21421 ggtactttca actataaggt atttcagaga tgagtatgaa gcacacatta aggataagaa
    21481 atgtccggcg ggagtatgca aggaactgct ggattacttt attatagccg acaagtgcaa
    21541 gggctgcggg atatgtgcca aaaattgtcc gcagcagtgt atctcaggcc agccaaagac
    21601 tccttacacc attgattcca gcaagtgcat aaaatgcgga gtttgtatgc agaagtgccc
    21661 attcaaggcg ataataagga aatagagaag agttaaaaaa caaaaaagct tcaaaaaggc
    21721 tgtcttagtt gaaaacgctg aggcggcctt tttcatcaat atcaataatc agatattccc
    21781 tggaattttc acgacaaatt tcctttgact tttcaaccag ctttgctata ttataatatg
    21841 tctgtgtgca ttagcattct tcacctcgga aatagtattg tcagattggt gaataatatt
    21901 agcggaagaa catattcaaa aaatgttaaa agattatcta gtaaaaacaa atacaactaa
    21961 aggattttgt atacacgcgt cgaatagtta ctaattgtga gtttataagg tcataatgtt
    22021 gggatttact tatttatata aattaagggg tgagcaaatt gaatataaga acactgacat
    22081 ttaagggcgg aacccaccca ccacacagta agaaggcaac tgaaagcctg ccgatagaga
    22141 gagccaatga acctaaggtt gttgtaatcc ccatgcagca gcatataggt gctccttgtg
    22201 aacctattgt gcaggctggt gatgaggtga aggttggaca gaaaataggg gaggctaaag
    22261 gatttgtatc agtcccggtg cattccagtg tatcgggaaa agttgcagta gttgagccga
    22321 ggctgtattc gggaggaacg gctgtgccct gtatagtgat tgagaatgac atgcagaaca
    22381 ccgtttcgga tcaggtggtt cccaagggag atatttcagt cctttcagct gaggatataa
    22441 agaacataat aaaagaagca ggtatagtcg gtatgggagg agctactttc ccaacccatg
    22501 tcaagcttgc tccgccgccg gacaaaaatg tggatacagt aatattaaac ggtgccgaat
    22561 gtgagcctta cctgacttca gaccacaggc tgatgcttga acatccggag aacgtggtgt
    22621 ttgggctgca ggcactgatg aaagccctgg gagtgaaaaa aggattcatt ggtatagaga
    22681 ctaacaagcc tgatgcaata gagaaggttt ttgaagcagc cagaggaatt gaggatattg
    22741 aagtagttgc acttaagacc aaatatcctc aaggggcgga aaagcagctt atttatgcat
    22801 gtaccggccg ggaggttccc tccggggggc tgccggctga tgccggagtt gtagtcaaca
    22861 acgtaggaac tgcggcggca gttgcaactg ctataaagac aggtatgcct ttaatagaaa
    22921 ggatagtaac tgttacaggg gaaggggtaa ataaccccaa aaatctattg gttaagatag
    22981 gaacatcttt cagggatata atagaacaat gcggagggct taagggcaag gtcggaaaga
    23041 ttattgcagg ggggccgatg atgggtataa cccagttttc actggatata ccggcaataa
    23101 agggcacatc cggtatattg gtgctttctg aagaggaagc aaggctgcct gaaccttcca
    23161 actgcattaa atgcggcaaa tgtgttgaaa catgtccgat aaaccttatg cctgtgaata
    23221 taagcatgta ctcccttgcc aacaggtatg acgaggctga ggctttaaat gctatggatt
    23281 gcattgaatg cggagcttgt tcttttgtat gccctgctaa gaggcctctt gttgattcaa
    23341 tcagggtggc aaaacgagaa atactggcta ggcgtaaaaa agcccagtct aagtgatggg
    23401 aggggaagaa atggaaaata gcagattttt agtatcatca tctccgcata taagatccgg
    23461 ggagacaacc caaaggatta tgctggatgt gataattgca cttatgcctg cgcttttggc
    23521 cggtgtattt tactttggat taaacgcatt gactcttacg gctgtcgctg tagtatttgc
    23581 ggtaacaact gaagccgtca tgcagaagat attgggaaag cctgttacag ttaatgattt
    23641 gagtgccgtt gttaccggta tacttattgc attcaacgta cctgtaacac ttcctctttg
    23701 gatggctgcc gttggttcaa tatttgcaat agcgattgcc aaacagtgct tcggaggatt
    23761 agggtataat tttattaacc ccgctttggc ggcgagagcc atgcttcttg cttcatggcc
    23821 tgtaaggatg acagcctggg ttgctccggg aacggatgca gtttctacag caactcctct
    23881 tgcacttgta aagatgggag cagatgctct ttcaggggct actcactggg aagagggtgt
    23941 agaagctgtg ggacaaacat taccgggctt atgggatttg tttatcggaa atatcggagg
    24001 atgcataggc gagacatctg cactggcact tatacttggc ggagcttacc tggtatatag
    24061 aagagtaata agtattagaa tacctgtttc atatatagct gcggtagcgg tgtttacatt
    24121 acttttcgga agatttgact ttgctgcagc aatttaccat gtattggcag gaggactttt
    24181 ccttggtgca atattcatgg caacagatta ttcaacatct ccaattactc caaaagggca
    24241 gataatattc ggaataggct gtggtttcct tacatcagtg atcaggtttt acggcggcta
    24301 tccggagggt gtttcatatt caatacttct gatgaacctg gcaacacccc ttatcgataa
    24361 atacacaatg cccaggaaat tcggggaggt gaagaaaagt gcgtgattat atcagattag
    24421 ccggaatttt gcttttggta tgcgttgtta tggctgcatc tctgggctac acaaatgcaa
    24481 taacctacga caggatacag gagcagatta taaaagcaaa cgatgaagca agaaaagaag
    24541 tgcttcctga tgctgacgca tttgaaattt tggatgacaa tgcattttct gaaattaaat
    24601 ccaatggaaa gtataacttt gtaacagaaa tatacacagc aaaagccgga ggaaatgttg
    24661 tgggctacgc tgtaaaggca gttccgaaag gctttggagg aactatagat gtagttgtag
    24721 gtattggtac ggatgggatc attcagggaa taaaagtggg aaataatact gaaactccgg
    24781 gacttgggaa aaattccgca actcctaaat tccagaatca gtttaaagga aaggcttggg
    24841 ataatacaat aaatgttatt aagagcggta ctccaaagga taatgaaatt gccgcagtat
    24901 caggtgcgac aattacatca agggcagtaa cggacggggt gaaccaggcc cttgaggcgg
    24961 caaaaaaatt atcaggcaaa tagaaaaaat ttggaggtga acaaaatgaa tctttggaag
    25021 gatttcagca acggccttat aaaagagaat ccgacattca ggttggtact cggaacatgc
    25081 cctactcttg cagttacaac tgctgtattt aacggcatag gtatgggtat tgcggccgct
    25141 gcagttctta taggctcaaa ccttatcatt tcgctgatta agaaaatagt accaaatgaa
    25201 gttagaattc cgatatatat tgttgtaatt gcaacgttta ctacaatagt tcagatgctt
    25261 atacaggcat tttcgcctgc actggacaaa tccctcggga tatatattcc tcttattgtt
    25321 gttaactgta taattattgc aagagctgaa gcatttgcag caaaaaaccc tgttgccaat
    25381 tccattatgg acggtttggg catgggtatc ggatttactg ttgctcttac aataataagc
    25441 tcagtcagag aaattcttgg tgcaggcaca ttcctgggaa tgccattatt tggtgaagct
    25501 tttgagccgt ttttgataat gatactgcct cccgggggct tcctggtatt tgggctttca
    25561 attgcggcat tcaataaaat taccagcctg agaaaaagtt aaggaggagg atatagatga
    25621 aattaggatt aattattatc agtgcaatat tcataaataa ctttgtcctc tctcagttct
    25681 tgggtatatg cccgttcctg ggagtgtcaa agaagataga cacagcagtg ggaatgagta
    25741 tagccgttac ttttgtaatg ggtttggcat ctgtaatatg ctgggcggtg caaatttttg
    25801 tacttgaccc catgggtata ggatatatgc agactatagc cttcatactt gttattgcag
    25861 gccttgtgca gtttgttgag atggttgtca aaaagacaag cccaacactg tatcaggcac
    25921 ttggcatata tctgccgctt attactacca actgcgcagt tttgggtgtt gcgatactga
    25981 atattgacaa aagctataat ttgattgagt cattaattaa cggcgtaggc gcaggtttgg
    26041 gtttttcact ggcaatagta ctttttgcag gtgtcaggga aagacttgaa ttggctccta
    26101 taccaaaagc cctggaaggc ttccccatag caatgatagc agcgagcctg ttttcaatag
    26161 ctttcctcgg attcagcggc ctgttcagcg gattgctgta gtattagtta tggaagaaag
    26221 tagaggtgac taccttgttt aatagtatag gattgcctgt aatagttctg gggggaatgg
    26281 cagttttttt cggtattgtg ctggcatatg catcaaagaa gtttgctgtt gaggttaatc
    26341 ccaaggttac tgaaatcaga gatgtattac ctggcgccaa ctgcggcgga tgcggatttg
    26401 ccggttgtga tgcattggcc gaggctattg caagcggaaa cgcgccgatt tcccaatgcc
    26461 ctgtaggagg gtctgagctt gcggcaaagc ttgcagtgat tatgggtgtt gaagcaggtt
    26521 ccgaggaacg tcaggtagca agagtaatct gctgcggtga cagtaacaat gcagcagaaa
    26581 aatatgaata tcaaggcata aaggattgca aggcagcaga aatgcttgca gggggttcca
    26641 aaagctgtcg gtatggctgc accggacttg gcacctgcgt aagggaatgt ccctttgatg
    26701 ctattcatat agttaacggt attgctgtag ttgatgaaga aaaatgtaca gcctgcaaaa
    26761 agtgcatagc ggtatgccct aaggggatta tcgagctggt tcccgtatca aaaaaggtca
    26821 gagtgctttg caggaatcag gataaaggca aagctacaat gcaagcctgc aaaatcggct
    26881 gcattgcttg ccaaaaatgt gtgaaagcat gcaaatttga tgcaataaag gttgaaaaca
    26941 atgtagcgag gatagattac agcaaatgtg taaattgtat gatgtgcgct gaagtatgtc
    27001 ctacaaaaac catatatgcc aatttcgcag acagaaaaaa agccttcata attgaggaca
    27061 agtgcatagg ctgcacaatc tgttcgaaga attgcaattt tgaggcaatt gagggagaat
    27121 tgaagggtaa gcataaggtg ctggacgata aatgcgtagg ctgcggccaa tgctttgcca
    27181 agtgccccaa ggatgccatt gaaatgaaat aaaagaataa taagccataa tagatttgtg
    27241 aataactccg actgtagggg aagtatgttc tcctcacctt ccaaagccag caaaatcact
    27301 ggcgtgggtc ccatctttgc gggtccatct ggttattttg cctatgaggt attcctgttc
    27361 cggggtcagg tggaaggttt cctcgaagat atcttcccca tgtcggtatt tgcctttccg
    27421 taacctcgta actggaagaa gaatgttgat attcttcttt tttgctttac ctctgttttt
    27481 tctttagctt tacgaattat ataaaatgtc gatattagat gtcaaatgaa ttccaaaaaa
    27541 gttgaagtcc ggtatttaaa atgttaaact aaaacataag gaattcacgt attgctgaca
    27601 tatgcagaag ttaataataa aggagatgaa tgtgttgaga agagaaggcg gcagaaacgg
    27661 gtggataatg cttctgatgg ttgtatgcgg attggtcgtg ggcggttttt tgggtgaact
    27721 gctggggaaa tactttccga tacttaagta tgggtacaac ctgggggttt ccgctcatga
    27781 gtggaatttc cacatactta gacttacctt tggttttgac ttcaatataa atatgttcag
    27841 catactggga attgtgattg caatttatat atacagaaag ctttagtgta aaacctatag
    27901 tacaggtgag ataaatgaga ataatactgg cttcaaattc cccaagacgg aaagaactgc
    27961 tgtctcagac aggcattaag tttgaggtca taccatcaag ttttgaagaa agtatagctg
    28021 aattgccggc ttcaaaactt gttgagcatt ttgcttatat gaaagcaaag gatgtggcga
    28081 agtccgtaaa gggagatgca ttggtaatcg gttcagacac aatcgtgtat tgtgatgaaa
    28141 taatgggaaa gccaagaaac agggaggaag ctttctgcat gctgacaaag cttagcggga
    28201 agcagcacct tgtgatatca ggcatcagca ttattaatac tgcaacggga gaaagcctga
    28261 ctgagcatga gagcacaagg gtacaaatca aggaattgag cagtgctgag ataacagcat
    28321 atataaacac cggtgaacct gaagacaaag cgggagcata tgctatacag ggtttgggtt
    28381 cactatttgt tgaagggatt cagggtgatt atttcaatgt cgtagggctt ccgatattcc
    28441 ggctgagaaa gatgctggag cacttcggag taaagcttat ttagcctgca tgatttgata
    28501 atccctggag gatttaatta taccatggag gagtaaagga tgcagagagg tcatgccaca
    28561 ataaaaaatc ttccggagga ggaaagaccc agagagcgcc ttgcaagaca tggggcttca
    28621 gtactttcca atgcggagct tctggccata ttgctccgga ccgggaccag ggaggaaagc
    28681 gcaatatctt tggcccatag aatactggta caggaacagg ggctgagata cctggcggac
    28741 agtaatgtgg aacagctgag cgctatcaat ggaatcggga aagcaaaagc tgcccagatc
    28801 aaggcagcga tagagctggg caaacgcctg gctgcttttg agcccggagc ggacaagccg
    28861 ctgaaatgcc cccaggatgt tgcaggcctg cttatggagg agatgagata tctcaagaag
    28921 gaacacatga agcttgtatt gcttaatgta aaatgcaatc tgatatcagt ggaggaaatc
    28981 tcagtaggaa gcctgaatgc ttccattgta catccgcggg aggtttttaa cccggcgata
    29041 aggaaatcaa gcgccagtat aatcatggtg cataatcatc ccagcggtga tccggccccc
    29101 agcagcgaag acatatcaat aaccgcaaga attgctgaag ccgggaagct tatcggtatt
    29161 gaactggttg accatattat cataggagac ggcaaatatg tcagtatgaa agaaaaagga
    29221 ctattctagt gtttttcagg tgtcaaaagt gaataacttg ttttccgttt gtaaaacata
    29281 tataaaaggt ttatataact atttttgcct atatacagta aaagctaatg tacaattgat
    29341 gtgtgtattt tgttaaggag gacatatatt atgggcttgt ttaatttttt gtcaaaggat
    29401 attggtatag atttgggtac cgcaaataca ctggtatatg taaggggtaa gggaattgtt
    29461 gtaagagagc cgtctgttgt tgctatgaag cttgatacca atgctgtaat ggctgtagga
    29521 gacgaagcaa aaaaaatgat tggcaggacc cccggtaata ttatagcaat aaggcctatg
    29581 agggatggag taattgccga cttcaatgtt acccacagaa tgataaagta cttcatagta
    29641 agagcctatg caggttcccc ggtaggaaag cccagggttc ttgtaagtgt tccttcaggt
    29701 attacggaag tagaaagaag agctgttgaa gaagcagcaa tgcaggcagg cgcacgtatt
    29761 gcaagactta ttgaagagcc gatggcagcg gcaataggag ccggtttacc cgtcggagag
    29821 ccaaccggaa gcatgatagt caatatgggc ggaggcacta ccgaaatagc tgtgatttcc
    29881 ctgggaggga ttgtaacgag caaatccata agggcggccg gagacaaaca ggatgaagca
    29941 attgtacatt atataaagag aatgtataat ttggcaatcg gagagcgtac cggcgaggag
    30001 ataaaaattg ttataggatc tgcttatcct aatacagcag aaggttcaat ggacattaaa
    30061 ggaagggacc tggttacggg tttgcccaaa acaataaagg ttacaagtga ggaaattctt
    30121 gaagctttaa gagaacccat tgcaataatt ctggacggca ttaagagtac tctggagaag
    30181 acaccccctg agctggctgc tgatataatg gacagaggga taatgctttc cggaggagga
    30241 gcgcttctgc acggattgga tattctggtg aagcacgaaa caggaatgcc ggtgcaggta
    30301 gccgagaatc cgttggattg tgtggcgatt ggaaccggca aagcattgga gcaatatgac
    30361 gaaataagca aatcatattc cgggaaacat aggagataag gttttgtgag gttttagtaa
    30421 tatgaaaatt aggaataagc tccttatttc tgttgtatta atatcaatac tattaatatc
    30481 attaattgct gttacatctc aaggtaggac aaggccgggt gtgattgatg gtatttttgg
    30541 aaggataata actcctgttc agagagtatt ctactcttcg ggggagtttt ttaagaatac
    30601 cttcaagagc ttatacgatt tgaaagacct taaaacggaa aatgaaaatc ttaaggcgga
    30661 ggtcgagaag ctgaaggcgc aaaacaggca gcttattcaa atggcattgg aaaacggcag
    30721 gctgcggagt cttctggaat tcaaggatac aaatactcag tttgaatata ttggagccaa
    30781 tgttaccgga agagatcccg gaaactggta tgatgtatat acgattgata aggggtccgg
    30841 ggatggaatt caagtcaatg atgccgtaat agtcggccat ggttatcttg taggaaaaat
    30901 tattgagaca ggtgcaaatt attcaaagct tatggctatt attgatgaaa gaagctctat
    30961 aagcataatt gtgaacagga caagggatat gggaattgta tcaggcagcg cggattctga
    31021 tgttatagca ataatggagc ttgaggcgga catagtcaaa ggtgacgata ttattacttc
    31081 ggagtacagc actttgccaa aaggcctgca cataggcaaa gtgaagagtg tggagaaaca
    31141 ggaaaaaaag ctgcagaagg tggtaattat cgagccatca gtagacttta aaaggcttga
    31201 agaggtattt gtaatcaaag cctccaagta aaatgtgagg gattgcttgt gagaatagcg
    31261 attatatgca tattggctgt ttttaatgtc atacttgaat cgacattatt tcaatgcacg
    31321 cgaattaatg gtataaagcc ggacttttca ataatgatta tagtagcata tgcaattatg
    31381 aggggaagct ccttcggcac ttttataggc cttggaatag gactgctgat tgatgttatg
    31441 tatgccagag tcataggcat caacgccctt tcttatatgg tgaccggata tataatagga
    31501 caagctcatg aaaaagtatt taaggacagc tttatacccc ctttcatttt taaccttatt
    31561 gcaattatca ttcttcagca cagctttttg ctagcttcat atttttccaa tgattttcca
    31621 agcgcaggga gttcatacat atatatgctg ctgcaggtga ttctgcctca ggcattatat
    31681 aatgcgatag cgggttcgat agtttacagg tttatatata agttggacga aacctctttc
    31741 atggacagaa ggatatactg atagacaccg gacatgagat gcaggttata gggagtagat
    31801 gataatatgt ttaagaaata ttttagtaat aggcttgata tcatcagggc ggtaacagtc
    31861 ttggtttttt tggttatggc gttcaggctg gcagacttgc agctggtaaa aggagaatat
    31921 taccggaaaa agtctgagac cttgaggaca aggaatattt cagtgactgc acccagaggg
    31981 catattgtag acaagttcgg gcggcttatt gcaggaaaca agcagagcta ttccgttaat
    32041 ataatgaaaa cggatgtacc ccaggataca ttgaacgata ttgcactgtc tgtaattaat
    32101 ataattgaac agaacggaga cacctacaag gatgaaatac caatacttat taatcccata
    32161 aggttttctt atcaggatga tgaaatcaac tggaagaaga agtacggtat tccggaaagt
    32221 gcatctgcaa aggacgcttt tatgaagctt agatttgatt atgagattcc ggcggatacc
    32281 attgatcttg aagcttatga aattatgagg gatgaaaaaa gcgttgaact gccttttgac
    32341 atcaatgagt ttgaattttc tttccgcaag tctgagataa aatggaagca aagccatggc
    32401 ttcgggcctg agaaaagtgc gtcggagata tttgatgtac tctgcagcaa atataaaatc
    32461 cccagagata tctatgatga tgaaaaggca aaaaaaatac tggcagtcaa gtacctggtc
    32521 ggacagaaca ggtataaagc atatgaaccg gtagaaatag ccgcaaatat caagatggaa
    32581 acaagggctc agatagaaga gagcaagatt tttcttcccg gagtggagat aatccagaag
    32641 ccaatcagat catatgcggg aagggatttt gccagccaca ttatcggcta tatgggtatg
    32701 atcggaagtg aacttgagga gttggcaagt aaaggataca ctccccagga tctgattgga
    32761 aagtccggca tagagaattc catggaagaa tatcttaagg gcaaggatgg ttccaggcag
    32821 atagaggtag atgtaaacgg gacgctgata aatacaataa atgaaataga tcctgttccc
    32881 ggtgatacgg tttttctgac tattgacgcc aagcttcaga aaacagcgga ggaatcattg
    32941 gcaagaacca tggagaatat aagaaatggc attgtgaagg gtaagccata tcctaatgca
    33001 acctccggag ctgcggtagc aatagatgtg aatacgggaa agattcttgc cctggcaagt
    33061 gaacccagat ttgatccgaa tctttttgcg gcaggaatca gcagtgagga ttggaagaac
    33121 ctgcagccta aaagcaagga tgtgtatgca cctaagccgc tgatgaataa tgcaatttca
    33181 tctatctttt cccccggatc aaccatgaag ctggttagtg ccgtggcagg gttggaaagc
    33241 aaggtaataa ctccttcgga aacgatatac gataaaggct attatacagc tatacccggg
    33301 gtatctccgt cctgttcaat atttaaatcc acaggcattc cccattacag tcagaatgta
    33361 tcccaggcaa ttaagaattc atgcaactat ttctttttcg aggtaggcag aagaatgggg
    33421 ggagaagtat ttgaagaata tgcgaagaaa ttcggcttcg gcgagaaaac gggtatagag
    33481 ctgctctctg agtataaggg aaccgttgaa ggcccgacga ataaaaaaga gctgttcaga
    33541 agatatcttg acaactatct tgtaaataat ctgcagataa atgataagaa tgtccgtgat
    33601 gaaataaaag gatacttgga taataatccg ggagaaagca aagtaaggaa aaggctcaaa
    33661 gaacttggta ttaatgattc aaaggcgatt gaacgggttt ggagatacat aagggacagc
    33721 aagtatcagc ccggagatgt gctgaacgca acaatcggac agggaatgaa cggtgttact
    33781 cctcttcagc ttgcaaatgc tgttgcgtct atagtgaatg gaggtacaag gtatagacct
    33841 tacctggtag ataaggtcat agactatgat ggtaacgtaa agctggaaaa aaagccggaa
    33901 gtggcggaaa agaatgatat ttctcccgcc aatcttgaag cgataaagag gggaatgtat
    33961 gctgttacca atgaagccgg cggaacagcg agaagtgctt ttacaggcag caaggttatt
    34021 atagcaggca aaacgggaac tgcggaagta gggaaaggct atgatgccca tgcgtggttt
    34081 gccgcctttg ctccctacga aaaacccgag attgccgttg tggtgatgat aacccagggt
    34141 ggtcacggaa actatcccgc accggttgcc agggaaataa ttgaagctta tctggctcct
    34201 gaggcgacta aggataatat gcatattgac aatgaaatat tgccgtaggg atgcattttt
    34261 tcattgggaa gaaggattta tcacaattat gaagaaatat tagactatgt atatcagggc
    34321 gatatatccg gaatcagggg gtactgccat ggcggaaaat gcagtgatat tcaaaggtac
    34381 aaaagacgga ttatatataa tactcaaaga agaaatggat ttgagcacca taaaatataa
    34441 tctggaaaag aaaatcaaac cttccaagcg tttttttgaa ggcgcaaata ttatgaactt
    34501 taagggaaag aagcttactc aggatgagtt tgatgagctt aaggaaatca tggagcagga
    34561 atatggcatg acagtaatag gaagttacaa cgataaggac tatcctgtac acgaagagca
    34621 tcgaggagcc aaaaagccgg aaatactgga acagcttcct tttgacaatg tgcataaggg
    34681 agaagcgctg atagtcaggg caacactcag gtcgggccag ttgattcaat ataaagggga
    34741 cgtagtggtg attggggatg tgaatcccgg agcccacatc aaatcagccg gatctgttat
    34801 tgtaatggga accgtaagag ggattattca ggcaggagcc aacggcgact atggggcatt
    34861 cattgtcgca tataaaatgc agccttccca gctgagaata ggagatataa taacaagatc
    34921 ccctgatgga atgggcagca agtcggataa tcctgagatt gcattggtca aacaaggaat
    34981 gatagttgtt gagccatatt tgaagaacag atagtttata tcatataaat atgctgatac
    35041 aataaattat ggaggtaata taatgggtga agtgatcgtt gttacatcag ggaaaggcgg
    35101 agtcgggaaa acaactacta cggctaatat aggcaccggt ttggctctta aagggaagag
    35161 cgtcgttctg gttgatgcag atatagggct caggaatctg gatgttgtga tgggcttgga
    35221 aaacagaata gtttatgacc ttgttgatgt tgtggaaggt gtttgcagac agagacaggc
    35281 actgataaag gacaagcggt ttcccaacct gtacctgctt ccggcagcgc aaacaaaaga
    35341 caagaatgcg gttaatcccg aacagatgca aaaactcacc ggagatcttg ccgaggagtt
    35401 tgattacgta atattggatt gtcccgcagg tatcgagcag ggtttcaaga atgcaatagc
    35461 aggtgcaaat aaggcgatag tagttacaac ccctgaggta tcggctgtca gagatgcgga
    35521 taggattata gggcttcttg aagcaagcgg attaaggaac ccaatgctta ttataaacag
    35581 gatcaagatg gatatggtca aacgcggaga tatgatgaac attgatgaca tgatagatat
    35641 actcgccatt gaccttttag gggtggttcc ggatgatgat ttgattgtcg tttccaccaa
    35701 cagaggagag cctgtcattg caatagaaca gtccctggcg ggacaagcct ataggaatat
    35761 tgtaaggaga gtactgggag aagatattcc ctttatgaat cttgaatcgg aggacggttt
    35821 tttcaagagg gtggcaaaac ttttcggatt gaataagaaa aagcagtaag agggggtgtg
    35881 agttatggat ttgttcaagt ggtttaacag aagtacaagc agtaaggata tagccaagga
    35941 aagattaaaa ttggtattga tccatgacag ggccaatgta tctcctcaat ttcttgaaat
    36001 gattaaggga gaactcatta aggtaatatc agattatatg gaaatagacg aaacagggct
    36061 tgaaatcaag cttacaagga caaaaaggga gattgatgat tcaacggtac ccgcattggt
    36121 tgccaatata ccgataaaaa gaatgaaagg caatgtgaat atttagaaac aggggaagga
    36181 cacgggacac ggggacgttt ctcctgtccc gctgaaagcg ggacaggaga aacgtccccg
    36241 tgtcccgtgt ccttttttta ttttttggga cataattcca ttggagtata aatataaata
    36301 tattaatcta taaagaacta tgggggaaag aatatatggg caacaataat tttgagaata
    36361 ttcccaggcc aaggtatgta aaggagtata gaaaactcgg tgcggccttc aaaacaaggc
    36421 ggtatggtga aaggaatttc ggagaaaggt ttctgatgca gtcctttata tctcttgcag
    36481 ccatagcagc agtactgtta gtgaataata ttaatattgg cattgcaaac acgatatctg
    36541 acggtataag aacaacgata aactggaata tggatttctc aaaggtattg gggacattca
    36601 gaaatgtcgg agatataata ccggaggcaa aggagaacct tggagttgcc caagtcggaa
    36661 tagaaagctt tgaagacagt ccaagcttta tatttcctgt ggaaggggaa ataacatcag
    36721 aatacggcga aagaatccac ccggtgttca agacagtgag gatgcatacc ggaatagata
    36781 tagatgcagc gatcggcaca cctattaagt cttccgcggc aggtacggta acaacagttg
    36841 gagaggatga aacaaacgga aagtatgtaa gtataaggag cggcaaatat gatgtgattt
    36901 atgcccactg ctacaagata acagttaaag agggacaaaa ggttcagcag ggagaaataa
    36961 taggggagac aggggacacg ggtattacct cggggcctca tctgcatttt gagatacaag
    37021 aggcagggaa gcctgtcagc ccaatggaaa tgctgatgga agtgcagtga acagtataac
    37081 cggcggcatt gggggttcac actgtgagga taaaactaaa tgtttttttt atcctttttt
    37141 tatttgtgag ctgcattata ggctggctgc agcagtcctt gataatgttt gccagtgtat
    37201 tgctccacga attcggacat gtattgacag ctaaaaagtc aggaataaag gtatatgaag
    37261 ttgagcttat gccttttggc ggaatatcca gaatggaaga attatcaaag ctgggagggg
    37321 ctgcagaagc tgtggtttca gctgcagggc ctgtcaccag ctttgtattg tcattgacat
    37381 tcagtttttt cagggagtat tcaggggtat tggaatcagc ctgcagatat aacctgataa
    37441 tttgcctgtt caaccttttg cctgtaattc cgctggacgg aggcaggatt gcaaggaatc
    37501 tgctttgctt cctgatggga tacaggcagg caacaaaaat tttatcgtca gcaggtaaaa
    37561 tagcagcttt tctgcttctg ggcttcaata ttcacatgct ggcagccgga agcaaaagcg
    37621 gggcgcttat aattgcggct gtattcatat atattggtgc tttaaaggaa gagaaaaaca
    37681 gctcgtatta ttacttgttt acggggaata acaccaagaa tgcgatgata gcccgggggc
    37741 gaataagaaa aaggtttata aaagtacagg aggataacct gataagacag atagtaaaca
    37801 aattcagccc tgtgacccag tgctgcgtgg aagtagcgga tgcaaaaggc aggcttaaaa
    37861 ggattctgag tgaagaagaa atcataaaag gccttcttca atacggatat gatggaaaaa
    37921 ttgcacaaat aattaacgga tagataaact ttcagtagaa ttttatactg caattatgta
    37981 ataatataaa ttaggttacg aggttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38101 nnnnnttcag tagaatttta tactgcaatt atgtaataat ataaattagg ttacgaggtt
    38161 acgaggtacg atatttaggg caggacttcg aggtcttggc aacactgaag acacgaaaaa
    38221 ccactaaatg cacggcggga taaatatttc gatacataga taattattga taatgatgca
    38281 aatatcgaaa tcttcttatg tagatatata acaaataata gttgccgtgt aatcgcgcag
    38341 cgattccgtg aaacatctgt gtgtttagtg ttacccgtta cctatgtcgc atttttagct
    38401 gtgtgccgat attataggag gaacaaatga aatacgataa caccagggag ttcatatatg
    38461 aggaactgct gtccaaggtg gaaaagccgg gaagatatat cggaacggag tggaacagca
    38521 tacataagaa tcctgaaacg gttgatataa gattcgcatt ttgctttccc gatgtttatg
    38581 aggttggtat gtcgcacctt ggaatgaaga tattgtatca cctgttgaat gatcagcagg
    38641 atattttctg tgagagggtt tttgccccgt ggcccgatat ggaagaagtg atgagaaaaa
    38701 ccggcataaa gctttttggg ctggaaagca gggattttat cgacagcttt gattttgtgg
    38761 gttttaccct tcaatatgag atgagctata caaacattgt gaatatgttg gatttggccg
    38821 gaatacccaa aagatccggc gaaagaaatg aaggccatcc ttttgttgtt gcaggagggc
    38881 cttgtgcata taatcccgaa cctctttatg acattgtgga tttcttcatg cttggagaag
    38941 gtgaggagca gctgctggag gtcatggagc attacaggaa gtggaaggac tcaaaaggca
    39001 ccagagaaga gttcctgaag atggcagcct ctgtcagagg agtatatgta ccgaagtttt
    39061 acaacgttga atataatgaa gattcaacca taaagtcaat aaagccaata aaagaggata
    39121 tccccggaaa agtcaggaaa agggtgataa agaacctgaa gggttcatat tttcctgaca
    39181 aaatagtagt gcccttcatt gatattgtac atgacaggat aatgcttgaa atattcagag
    39241 gctgtacaag aggatgccgt ttttgccagg ccggtatgat ttacaggccg gtaagggaga
    39301 gagaagtgga ggagctgttg gaaatagcgg acaagttgat acagagttcc ggctatgagg
    39361 agatttcatt gtcctccttg tcaaccagtg acttctcaaa gcttaatgaa ttggtggaac
    39421 ggctggtttc cagatataag agcagcagaa tcggattgtc tcttccctct cttcgaattg
    39481 attctttttc tctgaaactg gtccaggagg ttcagaaagt aagaaaaagc ggccttactt
    39541 ttgcgcctga agccggtact cagagaatgc ggaatgtgat caacaaaggt gtagacgaag
    39601 aggatctgct taactcggtg ggtgctgcat ttgaaacagg ctacagcagc gtcaagctgt
    39661 attatatgat aggccttccc ggagagacaa tggaagacat tcagggtatc gcagatttga
    39721 cattcaaggt agtggacagg tattaccagg tacctaagga gaaaagagga aagggcctta
    39781 atgtaaccgc aagtgcttcc tcctttgttc ccaagccttt tactcccttt cagtgggagc
    39841 ctcaggatcc catcaatact cttatagaaa agcagaggta cctgcaaaag gctattaaga
    39901 aaaagcagat aacctttaac tggcatgagc cttatgtcag ctacatggaa gcggtatttg
    39961 cacggggaga caggaggctc tgtgatgtac tcataagagc atgggaactg ggatgcaaat
    40021 ttgacggttg ggaccagtat ttcgatttcg aggcctggtt gcaggctttt aaagatacgg
    40081 gagctgatcc ggattcttat gccgctaggc gaagagagct ggatgaagta ctcccctggg
    40141 atcatattga tgtcggagtg actaagaagt atttgagggg ggagtacaag aaatccctta
    40201 agggagaatt gacgagagat tgcaggactg cctgcaccgg ctgcggtatt aatctgcttc
    40261 aggaaggcgg tgtctgtaaa tgatgcatat aagacttaaa ttcaccaagg gtaatgaagt
    40321 gaaatatata tcccacctgg atcttatgag agtatttcaa agagcattga gaagaacagg
    40381 catacccata tcctacagca gcggtttcaa tcctcacatg gagatttcct ttggggctcc
    40441 attatccctg ggtgtcacca gcaatgccga atatgtggat ttaaagcttg cggagccaat
    40501 ggatgtcagg gagatacaga ggcgcctgaa tgactcgctg ccggatggaa taaggatatt
    40561 ggacggtatt gagattaggg aaaaccacaa gagtgccatg tcggttgtct ctcattcaag
    40621 atatcgcatc agaattttta tggaaaacgt atcttctgat gagctgacaa agaaaattga
    40681 agaatttgca gcacaaaagg aaataaagat aatgaaggaa cagcccaaga agggctttga
    40741 actcaaagag attgatataa aacccctgtt agtcagtatg aagctgattg agtcgaaaga
    40801 agatatatac acaattgact gtcttcttct aagcggaagc agagggaacc ttaaacccgg
    40861 attattaatt aaggccttta aggaatttac gggatatgat attcaaagca tcaggataaa
    40921 cagggaacaa gtatacgggt taaaaaacgg caagcttttg gatttgcttg aatatgaaag
    40981 cgcagaagca taaaaaacca tgaatcgaag gatgaaggag cgcgatttat gaaagagatt
    41041 attattgatg tcggttccag gcaaacccgt gttgctttgc tggaaaacaa ggaactgacg
    41101 gagatatata ttgaaaggca gagcgaagaa aggattaccg gcaatatata caagggaagg
    41161 gtggaaaatg tgcttcccgg aatgcaggcc gcctttatca atattggttt ggataagaat
    41221 gcatttttat atgtaaagga tgcgcttcca aatacttatt ttgatgagga tgaggagtat
    41281 gtcagttctg cgcaatataa ggattatcag atagatgagc ttctaaaggc cgggcaggaa
    41341 ataatggttc agattatgaa ggagcctata gattcaaaag gagccagagt aacaacacat
    41401 attactctgc cgggaagata cgctgtattg atgcccactg ttgaatatat aggaatatca
    41461 aggcgcatag aggatgaaga aatcagggaa aagctgaaaa agctggcaga agagctgaaa
    41521 cccaagggaa tgggactgat aatcagaact gctgcagagg attgcaccga agcggattta
    41581 agcaatgata taaatttttt attgaagctt tgggataata taaaacaaaa gcaaaaaacc
    41641 ggcgccgccc caagaattgt acataaggat atgaacataa tatacaggac tgtcagagat
    41701 atgttcacca aggatattga caggcttgta atcaacaata aggaacacta tgagaaggtt
    41761 cgggagcttg taacaattat ttcatcacat tttaaaaaca aagttgaata ttacaataaa
    41821 agctatgata tatttgaata ttatcaggca gagcctcaga taagcaggat aatcaatcgc
    41881 aaggtctggc ttaaatgcgg aggatacatt gtcatagacc agactgaagc acttacatcc
    41941 atagatgtga atacaggaaa gtatgtcggt acggtggact tgaaggatac agtgcttaag
    42001 acaaatattg aggcagcaaa agagatagca aagcagctgc gcttaagaga tataggaggc
    42061 atcataataa ttgatttcat tgacatggcg gatgaaatcc acgaaagtac agttcttgag
    42121 gctctaaaaa atgcattgaa aaaagacagg acaagaacca acgtacttgg aataactcag
    42181 ctgggcctgg ttgaaatgac ccgaaaaaag gtgaggcagc gtatagaaaa gatactgatg
    42241 gcaccatgcc cttactgcaa aggagaggga aggattttat ccagtgagac aattgtcaag
    42301 aaaattgaga aggaagtaaa cagcttgttt tttaataccg gtgccatggc tgtttttctt
    42361 gagattcatc cggatgtgga agaattgata aactattata tagacgagga catgtttgag
    42421 ttgggtaaga gctttaataa acctgtattt gtcaggactt cggcagagct tcacagggaa
    42481 ggctatattg tgaaaggcat aggcatgtcg gaaattacgg agcttcgcaa aaagggctgc
    42541 agggaaattt aattgacaaa aatgttgcta tatgataaca tatattggcg taggcgtatc
    42601 cgcacgaatc gggtttaaat tctctaatgc aatgaggatg cctggaattc ggcgagactt
    42661 atttatgagg aggtgtagcc tagtgtatgc aataataaag accggcggta agcaatatat
    42721 ggttaaagcc ggtgatgtac ttgcggtaga aaagcttgat gcggaatccg gtgaaaaggt
    42781 tgtatttgaa gaagtacttg ctgtaggcga taacggtgag cttcaggtcg ggaatcctgt
    42841 agttgcaggt gcaaaggtcg aaggaacagt tgttgggaat ggcaaagcaa agaaggtaat
    42901 tgtattcaaa tataagccga agaaggatta taggaggaag cagggtcaca gacagccatt
    42961 tacaaaggtt aagatcgaaa aaataaacgc ataaaagcgg aaaaagcaac ggagttgttt
    43021 tttttgatta agattgtgat ctacaaggcc cgagagggca aaataacggg tttcaagata
    43081 tcggggcatt caggctacgg tactcatggc accgatatag tctgcagtgc ggtttctgcc
    43141 cttggccaaa cggctctcct ggggctgctg aaagttgccg aagcggaggt gcactacagg
    43201 attgaggagg gataccttga ttgcagtatt gccggtgcag gatcagacag aaagcgcata
    43261 atgtgtgatg cgatacttga aaccatgtat gaaggattta agagtattaa ggatagctat
    43321 aaaagacaca ttgatatagt tgaagaggag gtgtagatat gttcaagatg aaccttcagt
    43381 tgtttgcaca taaaaaaggt gtgggcagct caagaaacgg cagagactcc catgcacaga
    43441 gactgggaac aaaaaaggcg gacgggcagt ctgtgcttgc cgggaatata atagtaagac
    43501 agagaggaac aaaaatccat ccaggcataa atgtggggat tggtaaggat gacacattat
    43561 ttgcgcttat tgagggtgtt gttaaatttg aacgtaaagg cagagacaag aagcaggcaa
    43621 gtgtgtatcc ggtgcaataa tagcatagaa ttactgaagg tacggccagg ctgtaccttt
    43681 tttagcattt ttgcagtata ggagatgaga tgaatatgag cataaagaat agcggaatat
    43741 atgatgttcc tgacattgag tcggagaagg agcgttatat agagagtctg aaagagcatt
    43801 taagcgaaca gcgccacgac tttttcaacc tgcttcaagt attgtacggc tatactcagc
    43861 taaagaaacc agacaaggtg ctggaacaca tttctgaata ctgcaagcgg atggaaaaca
    43921 taggcaggct atataactgt aaatgtataa aactggccga tttactttac aataaggtaa
    43981 aagaagcgga gagcgcagat cttaagcttg aggtcagcgt tgaggtaagc tttgattctg
    44041 ctgtaaggat ccttgacgag gttcctgttg tatatgttgt agaccatgtg ataatgggat
    44101 ttttatatgt gcttgatgaa aagggtatga aaaatgccca tattatatat aacctgaaag
    44161 aaatttcaga aggatttctg atggaaattt actgcaggga gataaggaaa ggactgttcg
    44221 ggcctgttga ttttatcttt cccgagaagg tgatttattg gaaaaaagca gcaagaggtg
    44281 ttttgggatt cgataagata atggagtact gcagcgacgt taagcttgac ggaaaagtgc
    44341 ttggggacgg aacaaccttc attttgagta tttccaaggt caacaaagga gaataatatg
    44401 tttgtagata aggcaaaagt atatgtaaaa gcgggaaacg gaggagacgg cagggtatct
    44461 ttccgcaggg agaagtatgt gccaaatggc ggaccggatg gaggggacgg aggaaaaggg
    44521 ggagacattg tcttcatagc agacgaaaac atgagaaccc tgatggattt cagatataag
    44581 agaaagcata ttgccgaaaa cggagaaatg ggaggaacca ataattcatc cggcaagagt
    44641 gctgatgacc tgtacataaa ggtacctgca ggcaccattc tcagagatgc cgaatccgga
    44701 cttcttctcg gagatttgaa gaaccccggg gacaaggcaa ttgttgcact tgggggcagg
    44761 ggcggaaaag gaaatcagca ttttgccact cccacaaggc aagcgcccag gtttgcccaa
    44821 ccgggggagc cgggaatgga aagatggatc gagctggagc tgaagctttt ggccgatgtt
    44881 ggacttatag gatttccgaa tgtggggaaa tcaacattcc tttctgtagt aacttccgca
    44941 aaacccaaaa ttgcgaatta tcattttaca actcttgtac ctaatttggg ggtagtggat
    45001 ctgggagaag ccagaagctt tgtgattgca gatattcccg gacttatcga aggagcccac
    45061 agcggagtgg gattggggca tgattttttg aggcatgtag aaaggacaaa agtgctgata
    45121 catctgctgg acgcttcagg acttgagggg agagatcctc tggaggattt tgataaaatt
    45181 aacgaggaat tgaggctgta cagcgagaag cttgcgaaca agccgcaggt tgttgcctgc
    45241 aacaagatgg atattcctgc tgcgagggag aattatgcca gggtggcaga gacgcttagc
    45301 aggaggggat atgaggtgtt tgcaatttcg gcggcaacca gggatggtat tgcaccgctt
    45361 atggatagag tttttaacct gcttgaaagt acaaaagatg aagaaccggt tgaagaaatc
    45421 gtagatttga tgtatcatat taatacagag gataagccat ttactgccag aaaggaaaat
    45481 ggagtattta tcgtggaagg gaagctgatt gaacaactgc ttgcatcagt taacctggat
    45541 gataatgact ctatcaagta ctttcaaaag gtacttagaa agaggggtat cgttgatgag
    45601 ctgagacagt taggtgttaa agacggtgat acaattagga tgctggattt ggaatttgag
    45661 tattttgaat gaagaactgg aggtcaattg tagaatatga actgcatgca ttgcgaaaat
    45721 tgtaaacaga acaccgccac atatttctgc ctggcaaaaa acgaaatcat tattaacgat
    45781 agttttgttg ccaatatcga aaagagcaga aagggatgga aaaagggtga tcctggatac
    45841 gaaactcatc ggcgcaagct cagaaaagaa atagaagtat aaaaataacg gcagacataa
    45901 gtctgccttt tgaacataac ggcggagcag attatatgaa attgcggggg tgattttgtg
    45961 aaaaggaaag tggttgcggt aatcagcata ttagttgtca tagcctttct gttgacaatt
    46021 attgctccat tatttcaata gatttatgtt tcgcgggaat cgtgtttgtg catactctga
    46081 ataagaaata acagttgaca tatttgatgc atatgttata atcaacagaa atacatttga
    46141 ttcaaatcat atgagtatgg agatttatgc aatgaataat ttattaggga aaggaataag
    46201 cggaatatat tactttagct ttaacctggg ctattacttt agcgccggtt attatgcatg
    46261 caaaaaagaa tatattcctt ttcctgatga gttggaagaa tttaaaaatt attgtaagta
    46321 aaacttttat tcgtatgaaa tagcatcgat tcaaataagg ctcattagag ccttattttt
    46381 tttatcgtat aggaaataat aatttcctat acgatggggg atgcaagggg gagaagcccc
    46441 ctgccgataa aggagggtgc tcaggatgct tgcatccgtc gaagggaacc atagggttcc
    46501 ctagcgaaaa aaacgcgccg tgagacacat tttttagaaa atataaagga gggtaattat
    46561 ggtgatagta atgagagaaa atgcctcaga tgaagaaatc gaaaaactta gagaaaggat
    46621 agaaacagaa gatctcaagg taagtgtaat aaagggcagg gattattgtg tgcttggaat
    46681 tatcggagat actaccggta tagacataag tgaaatcgaa gcaaatgaaa gcgtggaagc
    46741 ggtcatgaag attcaacaga gatacaagct ttcaaacaga atgtttcaca aggacaactc
    46801 ggtaatacag gtaggcgaca agaccatagg cggtcaggaa ctggctgtaa tagcaggccc
    46861 ttgctcggtg gaaagcgaag agcagatatt ggaattggcg aaagaggtga aagagtacgg
    46921 ggcaggcttc ttaagaggag gcgcattcaa gcccaggaca tcgccataca gcttccaggg
    46981 catgaagctt gagggacttg agcttttgaa acttgcccgg aaggaaaccg gccttccgat
    47041 tgtcacggaa ataatgtcag tggatatgat agagagattc gtagaagatg tggacgtaat
    47101 acaggttggc gcaagaaaca tgcagaactt tgagctgctt aaggagattg gaaagaccac
    47161 aaagcccata cttttgaagc gcggactgtc tgcaacaata gaagagctgc taatgtcagc
    47221 agaatatatc atgtctgagg ggaacagcaa tgtaatactt tgtgaaagag gcataagaac
    47281 cttcgagacc tatacaagaa atactttgga tatcagcgct attctggcaa tcaagaaact
    47341 cagccatttg cccgtaatag ttgatccaag ccatgcgacg ggcttgtggt ggatggttga
    47401 acctctggca aaagcatcgg ttgttgcggg tgctgacgga attatggtag aagtgcacag
    47461 cgatcctgca aatgccaagt gtgacgggca gcaatccatt aagccggaaa agttcagggc
    47521 aatgatgaaa gatattacat taatggcgga ctcgctgaaa aaggctaatt aaagggcgga
    47581 acataaagaa aatactggga atggtatcag ttaagtgttg cggtgattgg aggaattaaa
    47641 ttggaagagg gtgactttag aaggaatatt gcagtagttg gcttgggatt aataggcggt
    47701 tcttttgcca tggcactgag aaagcataat gcagtaagaa tattcggcat agatattgat
    47761 gagagcgtgt tgaacaaagc agccggctgc ggagtaatag acgagggtgc ttccaatgca
    47821 aaaaaaatat tatgcaaatc cgatatggta ataattgctt tgtatccgcg ggaagcaatt
    47881 gaatttatta agaattatgc cgaatatttc aaaccgggga ccattgttac cgatacttgc
    47941 agtatcaagc agccgataat tgaagctgca aagaaaagct tgcggcagga tgtggagttt
    48001 gtcgggggac atccgatggc gggaaatgag tttcaaggtt ttgaagccgc atcccgggaa
    48061 ctcttttttg atactaacta tattatcaca ccccatgaaa gcaatagcga aaacgggata
    48121 atagctgtgg aaaagctggc agcttcaata ggatgcaagt gtttgaccag gataggagca
    48181 gaggctcatg accgtttgat atcctttaca agccagctcc cgcatatagt tgctatctcg
    48241 tttgcaaacc tgatgatgag ggagaaaggc ataagccctt ttaccgggcg cagcttcaag
    48301 gatgccacca gagtggcggc attgaataag gaactctgga ctcaaattct caggatgaat
    48361 aatttgaata ttattgataa aatagaagat atggaggaca tgcttcataa attaaaagat
    48421 gctataaaag aacaggacga aagcagcctt caaatactgt tcgacaatgc tattaaaagt
    48481 aaaagggaga ttatatagat ggtgactata gaggtttcaa taaaaagcag cagcaaaaaa
    48541 tataacatcc aaatcggcaa aaacattcgg caggagcttt ataaatatct ttcagagaga
    48601 tataggggca aaaaggctgt aatagttacc gatgataatg taaataagct ttacggagca
    48661 tcattatata ggggtatagt tgaggcaggg atgaaatgca ggcttatatc ctttagtccg
    48721 ggtgaaaaaa gcaaatccct gcaagtcctt tcggggatat atgaggatct tgccggtttc
    48781 aacgcagaca ggggagatat cattattgcg tttggcggcg gggttgcagg cgatttggca
    48841 gggtttgccg cagctaccta tttaaggggc atatcgtata ttcaactccc gacaacatta
    48901 ctggcgcaag tagacagcag cgtaggcggt aaaactgcaa ttgacctttc ctcggggaaa
    48961 aacctggcag gaagctttta tcatcctgaa attgtattta ttgatacttt atttctgaat
    49021 acactggaag acaggtattt gagggacggc atggctgaag taatcaaata cggatgcata
    49081 aaggacaaga gcctctttga caggctttcg gattatgaaa acctgcagca atgtattgcc
    49141 gatatggatg aattggtatg cacctgctgt tcgataaaaa gagcagtagt ggaagcggat
    49201 gagctggaca gaggggaaag aatgcttctt aatttcggtc atacctttgg ccatgcagta
    49261 gaacaatatt ataattatga gaagtatacc cacggggaag ctgttgcaat gggtatggcg
    49321 gaaattacaa aacggagcga ggagatggga ttaacagaac ggggtactgc aaaagctttg
    49381 gcggatatat taaagaaata tggcttgcct tatgaaatcc ctgttgcaaa taaaaaagat
    49441 ctgataaatg ttatgaggcg ggataaaaaa ttcagcggtg atagtataag gcttgtatta
    49501 ttaaataaac tgggaactgc ctttataatg aaagcaaaat ccgaagaact tgaaagcttt
    49561 cttgatacaa cgggagatgc aagggaggat taaggatgaa agaagtgaaa atatatcccg
    49621 gcagccttgc cggagaggtt ttcataccag cctcaaaaag cataagccat agggcggtga
    49681 tatgcgcagg actctcggaa ggaataagta acatatataa tgtcggaata tcgcaggaca
    49741 ttgaagcgac tatagaagca ttgagaagct tcggtacaat gattgaaaag gcggatgcct
    49801 cactgaggat aaaggggagt gccgtaccta agctgaaaaa ctccggaata aattgtcatg
    49861 aatcaggttc tgccttaagg tttattatac ccattgcagc cctgacgggt gaacccgtta
    49921 ctttttacgg agaagggaga ctggttgaaa ggcctttgct gcaatattac aatatttttg
    49981 agaagcaggg gataaaatac gaaaccgagg caggaaagct tcctcttcac attaacggca
    50041 ggctggttcc cggagagttt caactggagg ggaatatcag ctctcagttc attagcggct
    50101 tgatgtttgc actgccgctt ttggaggggg attcactgat aacggtaacc acagagccgg
    50161 agtcaaagcc ttacatagat cttacactga aggttctgaa ggacttttca atacatatag
    50221 aaaaccatgg ttacaggact tttataataa aaggaaggca aaagtacaaa gcgacggatt
    50281 ataccgttcc cggagatttt tcacaggctg ccttctggct ggcagcggga gcattagggt
    50341 cggaggtggt atgcaagggc cttgatataa attccatgca gggtgacaga gtaatgatgg
    50401 acataattgg cgaaatggga ggcggaatat ttataaaagc tgctgctgtc agggcgcttc
    50461 cggctgatac aagaggaaca gttatcgacg cctcccagtg tccggatttg gtcccgatat
    50521 tggcagttct gggggcactg agtaagggaa ggactgaaat agtaaatgcg ggccggctgc
    50581 gtttgaagga atccgacagg ctgaaggctg taagcagcga gcttggcaaa ctgggagcgt
    50641 gtattatgga attgcatgac agcctggtta ttgacggcag aaaaaacctg cggggcggaa
    50701 gggttgaaag ctggaatgac cataggattg caatggcact ggctatagca gccacaaggt
    50761 gcgaagagcc ggttatcata agcaatgctt cttgtgtgaa aaagtcttat cctgatttct
    50821 gggtacatta taaagcattg ggggggaaaa tcgatgagcg gtatatgggg taacagctta
    50881 aaaatatcaa tatttgggga atcccacggt aaagcaatag gaataaatat cgacggcttg
    50941 aaagccggaa tccttctgga tcttgacttt ataagaaggg aaatgcaaag gcgggttccg
    51001 gggaatagtg agtttgccac gccacgaaag gaaggagacg aattcgaaat tttaagcggc
    51061 ttttttgagg gcaggactac aggaacacct ttatgtgcag tgatacataa tacagataaa
    51121 aaatccgaag attatgaaga gctgaaaaat ataatgaggc cttctcacag tgactacaca
    51181 ggttatgtga agcatggcgg cttcaatgac tatagagggg gagggcattt ttccgggagg
    51241 ctgactgccc cgctggtttt tgcgggagcg atatgcaaac agcttcttga aagcagggga
    51301 ataaccatag gcagtcacat aaggagcata ggagaagagc aggacaggcc tttcaatcct
    51361 gtcagcgttg actcaggcac attaaaaaaa ttggcgggca aggcttttcc cgtattggat
    51421 gcaaaaagcg aagcccttat gaaagaggtt atcgcccggg caaaggagca aaagaactca
    51481 ataggcggtg ttgtggaaac tgcagtactg ggcgtgaagc cgggaatcgg agaacctttt
    51541 tttgactcag tagaaagcac tttagctcat ctcatatttt ccattccggg agtaaaaggc
    51601 ctggaattcg gagcgggttt tgatatatca aagcttatgg gttccgaggc aaatgatgag
    51661 tattatatgg atggagagga agtaaaaacc tacagcaaca acaatggggg aatacttgga
    51721 ggcataaccg acggaatgcc gataatattt cgaaccgcca taaagcctgt tgcctcaatc
    51781 ggcaggcggc agcgggccat agatattgag aagaaagaaa acgtcggatt ggaagtacag
    51841 ggaagacatg atccgtgcat tgtgccgaga gcagtgcctg tagtagaggc agttactgct
    51901 atggcacttt tagaccttat tatggttagt cagggaaaat gaatgaatag agggtgtgtt
    51961 tgcattggat gaacttgaaa tcttaagaca gaagataaat gaaactgatg aaaagcttgt
    52021 gaagctgttt atagaaagaa tggcacttgc caaaaaggtc ggggagtaca aatcagggaa
    52081 aaacattagg atatatgatc cggcaaggga aaaagacatt gttggcaggt ttgtcaatag
    52141 agtcagagtc gattttgata taaagtatat tgagcagttt ttggaaaacc tgatgttttt
    52201 gagcaggagg cttcagggag atataatagg cagaaagcag gaaccttcta agataaaaaa
    52261 cctcaatacg aacatggcaa taggctatca aggggtgccg ggctgctata gctatcaggc
    52321 ggggcttgaa tatttcgggg aagggattga ggccttgagc tatcacagct tcagggatgt
    52381 tttcgatgct cttgcaaaag gggaaataca gtacggcata ctgccgatag agaataccac
    52441 ctccggcagt attaatgaag tatacgattt acttagagaa tatgaattct atatcgtagg
    52501 cgaaaaatgc ctgagagtgg agcacagctt acttgcggtg gagggagcaa agctatctga
    52561 tattaaggag atttacagtc accggcaggg tttgattcag tgcagccgtt atctttcaca
    52621 ataccccgaa tggaagcaga taccctattt tgataccgca ggaagcgcag agtacattgc
    52681 gaaggaaggc ttgaaatcca aagcatgtat tgcaggtgag aaggcagctt cagtatatgg
    52741 cctgtgtata atttgccggg gcatacagga taacaggaac aacagtacca gatttgtaat
    52801 tattagcaaa gaaaagcttg ataatgaaaa ggctgataaa atcagcatag tattcagtgt
    52861 gcctcataag ccgggaagcc tttatggaat attaaggtat tttgcggagg gtaattgcaa
    52921 tttgatgaaa attgagtcca ggcctttgga agataaggca tgggaatatt tcttttatac
    52981 ggatttcagc ggaaacataa atgaggaagg tataagaggc attctggcgg atatggagaa
    53041 ggaatgttca tatttcagat tgctgggtaa ttattgaagc gaggttgggt gaggatgatg
    53101 aataatgttt tcggattaat cggagaaagc ctaagacaca gcttttctcc gcaaatacat
    53161 tgtgaaattt tcaaaatgct gggaatagaa ggagaataca ggctttttga acttcaggaa
    53221 gaggaattgg agcctgcgct cctgaagtac agggagcagg gcatcaaagg attgaatgtt
    53281 actgtaccat ataaaataaa gattatgcct taccttgatg tgatttctga agaagctgaa
    53341 aaaatcggtg cggtaaatac aatttcactg gccaatgatg tactcacagg gtataacacc
    53401 gattacaggg gattcggcat gctgcttgca agaagcaata tagaggtaaa aaataaaact
    53461 gcggtaatac tgggtaccgg tggagtttca aaggcagcag cgcagtatct catggataat
    53521 ggagtaaagc gcatcacata tgtgagcaga aatcccggtg atggggctgt cggctatgat
    53581 gaaacagaaa gcctgaaggg ggatattata ataaattgca ctccctgcgg aatgtacccc
    53641 aaggcggatg tatcaccggt tccttatgga acatttaagc agtttggtac agcagtagat
    53701 ttaatatata atccggaaaa gacaatgttt ttaaagcagg ctgaagaaaa cgggctgaaa
    53761 gcggtaaacg gactttacat gcttgtgggc caggctgttg cagctcagga aatctggaat
    53821 ggaatcggac tctcaaggtc acaggtggat aatatttacc agcatataaa aaagatgctc
    53881 tataaggcag acacaagcaa gaacatagta ttgataggac tatcgggctg cggcaagacg
    53941 tctataggaa ggctgcttgc agaaaagctt gagaggaagt tcatagatat agatgatatg
    54001 attgaaaaac aagagggatg tactattaac gagctgtttc ggcaggggga agagcacttt
    54061 cgtaatctgg aaactaaggc ggtgcttgct ctggagaggg aaaaggcttt agtcatttca
    54121 accggaggcg gtgtggtaaa aagagctgta aatatgaaaa gcctgaaaaa aaacggaatt
    54181 attatattta ttgacaggga tgtatgtgat attatggatg acattgatac aagcacgagg
    54241 ccgctgctgg caggggggac aggccggctg ctgcagctgc atgccgaaag gtatgatttg
    54301 tacaaggagt acagtgattt cacagtttgt aatgacagta aagctgcgga tattattgaa
    54361 gatatctatc ggaaattagt ggaaaaactt taaaaactct gtatattatt gtataatgga
    54421 cgtaattcat aaaaaagtac agaggtattt ttatgtacga ggatgcagtt gacaatgggc
    54481 aaagcaggat tgggattatg ggaggcacct ttgacccgat tcattacgga caccttgcgg
    54541 cagccgaggc agcgagaata gaatttgggc tctgcaaggt catattcatg cctgtgggaa
    54601 accctcccca taagcagtcg caagcgataa gcgatgcaga gcacagatat cgtatgacag
    54661 ctctcgcaac ttcttctaac tccgggtttg aagtgtcccg cctggaagtg gataaggccg
    54721 ggattacata tacttttgat acaatgaagg aactgagaaa catatatggc gaagctcccg
    54781 caatatattt tattacgggg gccgatgcag tgctggagct gctgacgtgg tacaagttag
    54841 gggagctttt gacgctctgc aaatttattg ctgttaccag gcccggattt gatatttgta
    54901 agctggaaca aaagatagcg gaaattacgt ctaaatatga tggagaaatt atatgcctgg
    54961 aagttccatt acttgaaata tcctctacag atatcagaga aaggataagg agcggaaagc
    55021 ctgtaaaata tctgctcccg gaagaggtgg aagcatatat acataaaaac ggactttaca
    55081 aggagtgaat tatttgctgg atttgaattc aataatggag catcttgaaa agaccatatc
    55141 ctcaaagaga tatatacatt ctgttaatgt gagtaaaaca gctaagaagc ttgccgaatt
    55201 ttatgaatac gatgcagtca aggcggaaat tgcaggtttg gtgcatgatt gtgccaggga
    55261 ccttgaaaag ccccttcaac tgaaatgcct taaggaagaa ggtattgagg cggatgaact
    55321 gactttgaat attgaggagc tgttgcacgg ccctgctgca gtacatattt gcagaaaagt
    55381 ttttgccatt gaggatgagg agatattaag cgctgtaaga tatcatacaa ccggtaaaga
    55441 gaatatgtca cttcttgaaa aaataatcta tctttcggat tttatcgaac cctcaaggag
    55501 ttttcctgga gtggaagagc tgaggggact agccttcaag aacctggaca aagctctgct
    55561 tctggctttt aattcgtcta tcctgtacat attgtcaaaa aacgaatatg tgcatattaa
    55621 tacaatactt gcaagaaacc atgtgcttaa agaagtcgga gagaatggga tgcagtatca
    55681 tgacagcagc agatagaaat atcactgtgt ctggcgtaaa atatttagaa aacgaggttt
    55741 acaagcttga agaaatatat aataattttt ttggcatcgt ttttagtttt ttttgcaata
    55801 atcggcggag tatataataa atataaatca aatatgactg atgagcactt tgtcaggacg
    55861 attgaagaga atcccgaact tgagcccgaa gacacgaaag aggatgtaat aataaacact
    55921 ttggtaatgg gtattgatga agcaagatct gatactatga ttgtggtcag ctataataag
    55981 gaaaaccaaa aaattgcatt gctttccata ccaagagata ccagggttga gattccggga
    56041 tacggcattg acaagattaa tgcggcagct gcaaaaaaag aaggtactgc tcttgccatg
    56101 cggactgtgg gtaatcttct ggatgttccc atacatcatt atgttaaggt aacttttaaa
    56161 ggagctgaaa ggataattga tatattggga ggagtatcgg taaatgtgcc ccctggtatg
    56221 gattatgagg accctgttca ggatttgtat attcatttaa aaccgggtaa acaagtgctc
    56281 aacggtaagg atgctgtgaa atttgccaga ttcagatcgg gatatcccga ccaggacctt
    56341 ggcaggatta gagcccagca gcagcttata aaggcattta tcgacaagct tacaagcccc
    56401 aagatgatac caaaagcact tacaatcgtc gatgcaatgt ccaaatgtat taagaccaat
    56461 cttgaaagca gtgatatagc acactatgct attaatatta aagacataaa aatggagaat
    56521 gtgagttttt atacacttcc gggcagcgac ggatacataa acagagtctc atactttttg
    56581 catgatgagg ccaagctcaa ggagatgata cagcagatac ggatagatct tggagtagag
    56641 gagcctgcag aggagatgga attgctgccg gcagataatg ataatgctcc ggagggggcg
    56701 gaaagcggta ttggcttaag atgacataag ttagtattga caatggttat aaatatgata
    56761 taacaattgt aagataatag agataaacgg aggggtgcat ttgttttcgg tatcaacgga
    56821 aatggcgaat aaagccatcg aactcttgaa gggcaagaag gcaaaagata taaggctatt
    56881 ggatattcat gagcttacta ctatatcaga ttactttgta attgctgcag gaagctctac
    56941 aacacaagta caggcaatga gcgatgaatt ggaagaaaaa atggaacttg caggttatga
    57001 aatgtatcat aaggaaggat tcagaaacgg cagatggata ttacttgatt atggaaatgt
    57061 aatagttcac ttgttccatg atgaagaaag gcaattctat aatcttgaaa gactctgggt
    57121 agatgcaaaa acggttcttg tggataatga atagataata attctatatt gtttaggggt
    57181 gttagtatga gtaaagacct gcatcccata cttaaaggaa tcataccgct gatagatggg
    57241 atagcaaata catttggaaa aaactgcgaa gttgtactgc atgatatcag aaatccgcaa
    57301 agctctgtta ttgcgatagc caacggacac ataaccggaa gaagcatcgg aagccctatg
    57361 actgagtatg gtttggctac actgcgcagc ggacaattcg acaagcctat agtaaactat
    57421 agaaagaaga caagggacgg caggattctg aaatcctcgt cattgtttat aaaggatgaa
    57481 aatgggaagc ttattggttt tctgtgcatc aattatgata tttcagagct tacaatagcc
    57541 agaaacatta ttaataatct ggcaaatgtg atcgaagaaa cggatttcag cgaagaagat
    57601 gaatgttttg gaaacacggt aaatgagatg ctgagcagta tagtgaataa ggcattggaa
    57661 tccgtaggga agccggttgc cttcatatct aaagaggaaa aggtgaatat agttcagttg
    57721 ctggacgaaa agggtgtatt cctgataaaa ggagccatcg actatgtcgc caaggttctt
    57781 tgcgtatcaa gatatactgt ttataattat ctggatgaga cgagggtaaa tgattaataa
    57841 tgaaagaagg gatatataat gacaaaagaa atcatcagtg ctgaaaaggc accaggggct
    57901 ataggcccgt attcccaggc tgtaaagtac gggaacatgg tatttacttc aggtgcgata
    57961 ggagttgatc ccgctacagg tgtatttccc gaggggggta tccaggttca ggccaaacag
    58021 tgccttgaga atctcaaggc tgtacttgaa gcttcaggga gcagtttgga gaatgtgctc
    58081 aagactacag tatttattaa ggatatgaat gatttttcaa aaatcaatga aatttatgga
    58141 caatatttta ctgagaagca tccgggaaga tcctgtgtgg aggttgcaag gctgccgaag
    58201 gatgcgctta tagaaattga agcagtagca tatataggat aaagaacatg tgtgggacac
    58261 ggggacgttt ctcctgtccc gctggggaga cacggggacg tttcgcgtgt ctcacaaagt
    58321 gagacagggg aaacgtcccc atgtctccca gtccccatgt cccacccgtg cccttatctt
    58381 tttatataag tcacgtttct gtattttttt ctgcgtctta ctttatatat ggtgactact
    58441 gtccgataca gcagaaaacc tgcaatggcc caaagccacc acaagattgg cttcttaaca
    58501 gcttcgactg atacagagga gaggagttct atcttcgtta cttcattccc ctccgcataa
    58561 acaataattt ctcccaatac ctcacctttt tcgataggct ccctcaaccc atccctggtc
    58621 ttaatttctg tcgtaatatt ctctttttca gattcggaaa gcaccattga gaaatcctca
    58681 gcagcaagga gatttatttt ttcctggcct tcttttattg gcacactagt tactatctga
    58741 tcctttttca gtatttgcac cttctgaaag ttggaaaaac catattcaaa aagggctaca
    58801 gtgtcactgt acatgttttg cccttcggca cccatgacta cggatatcag ctccaggtct
    58861 cctttctttg ccccgcctac caggcactgt tttgctttgg tggtatagcc ggttttgatg
    58921 cctgtggcat actcatatct gaattgatcg tatatttccc atataagttt gttggagttt
    58981 gttatatagt tcctttcctc ctgtttattt gtctttggaa tggtatattt gacttctttg
    59041 accacatccc ggaacttatc caaagtcatt gcgtactttg caatcatagc aagatctctt
    59101 gcagtcgtat aatgattatc attgtgcagg ccattgggat tgacaaagtt tgtgcttgcg
    59161 gcacccagtt ctctggcttt gctgttcata agttttgcaa attcctccac agagcctgaa
    59221 atgtgctctg ctatggctac ggcggcatca ttggcagatt ccagcatcaa ggcataaagc
    59281 agttgttcca tggtaagctg ctcccccggt acaaggtaaa tctgactgct tccaatttca
    59341 acaaggggag gattcttgcc tatagtgact atttcgtcca gcttcccgtg ctcaactgcc
    59401 agaagtcctg tcatcacttt tgtggtactt gccggataac gcttctcatc cgcgtctttt
    59461 tcgtacagga tctttcctgt ctcggcatct atcagaatgg ctgacggagc ggtaacctca
    59521 ggcacatctc cataaacccg tacaaaaggc atcatcaaac ttaatatcat aatatatgca
    59581 attaatcttt tcatccattt ctccttgtga atcatgttta tcccgtaaat tgaatgagtc
    59641 ttgtgccaac gatcactatg tgcctcgtaa agcatatcac ttcttgagca tgcgaggcac
    59701 tgaataggtg ttgtgattgt taacacggtg aaaaatccaa aagctgtcgt gttaacaatc
    59761 actagtatat caaaaaagct tgccgatggc acccctttgg aaagggtttt ttgaatattt
    59821 gaaatggaaa tatatgatat aaataatgtt acatggaaaa gaaggaaatt aatagaaaat
    59881 gcagaaataa gtatattatc aatgagatga aggagggtaa aggttcaaga tgctttatat
    59941 cacaaggaca caaaaatcaa tacttgcatt tgtaatactt gttctggtca taataagcgg
    60001 tactatatat ataagacagc aaaaggccac ggaaataagc cttgctgagg ctgtaccgga
    60061 tcaggcggaa gcacaggctg aaacaaagcc cgcggaaata tccgtatata tctacggttt
    60121 tgtcaagaat cccggagtag taaaagtcct ggagggaacc cggcttgatg aggctatcag
    60181 gctggtgggg ggagctgttg aggaagctga tttggaggct gttaatcttg catacagact
    60241 tgctgatgaa gatgcgatat acatcccaaa gaagggtgaa agccctcaag acacaggaaa
    60301 ggcaattccg ggggtcaata cggtcaagag tgcggcagtt aacaagccgg gaaaggtaaa
    60361 tattaataca gcaggtgaaa aagagctgga tactcttaag ggagtcgggc cggctacagc
    60421 aaaagcaatc atagaataca gggaacagaa agggctcttc aataccattg aggatatcaa
    60481 gaaggtaagc ggtatagggg aagcaaagtt caacaacatg aaggataaga taacggtaga
    60541 atagaagaat gcttcacaat aagtttgtga agtactccga ctgtagggaa agtatgttct
    60601 cctcactttc caaagccagc aaaatcactg gcgagggtcc catctctgcg tgcccgtctg
    60661 gttattttgc cagagatatt cccgttccgg ggtcagatgg aaagtttcgt caaagatatc
    60721 ttccccacgt cggtattctc atttccttta tatacaaggc agtattctgt aagttgtata
    60781 accaggcatg agcttgtata gtatagcagt aggcctgtca aaatcataat ccgtgtgcct
    60841 cgatatacta tactgtctct tgcggagggt tatacaaact tcagatatgt tgcagttttc
    60901 ttctattatg caatgaatat accgtaaaac atttagtcaa aaattcttgg aatatatgtt
    60961 ataataatgt ggaaattgaa atatagcaag aagttgaaag gaggcggcat tatggatagc
    61021 aaaaacaaaa caaggcttac acaaatgacc aaaagctccg gctgagctgc caagataggt
    61081 ccggagaccc tggcgcaggt tctgtgccag ctgccaaagt tttacgatga aaacttgata
    61141 gtaggtattg atacctccga cgatgctgcc gtatataaga tagatgaaga taaagctgtg
    61201 attttgacta tggatttttt tacgcctatt gtagatgacc catatacatt tggacagatt
    61261 gcagcggcaa actctttaag tgacatatat gctatgggcg gaaggcctgt tactgcaatg
    61321 aatatagtat gctttcccac ttgtctgccg attgatattc taaaagaaat acttaagggc
    61381 ggagcggaca aggttaaaga agccggcgca atagttgtcg gaggacattc cgtagaagat
    61441 gatgaaccga aattcggcct ttccgttatg ggaatggtac atcccggcaa ggtaaccgcc
    61501 aattccgggg caaagcccgg ggatatgctt atacttacaa agcccttggg cattggtata
    61561 ttgaatactg caataaaagc ggatattact gatcaagagg catataaaaa ggcagtggat
    61621 gttatgtcat accttaataa ggatgcctgt gaagcaatga tgaaggttgg tataaacggc
    61681 tgtactgata taacaggctt cggccttttg gggcatggtt acgaaatggc tgaagcaagc
    61741 aacgtttccc tggaaatatg gtcggattat attcccatta ttaaggaaag cattgatttt
    61801 gcaaaaatgg ggataattcc tgcaggtgca tacaataatg aaggatttat tggagagcat
    61861 atcagatttg ataaaagcat aaagcaggaa gtcagggata tcatgtacga ccctcaaact
    61921 tcgggcggac ttcttatatc agtgcctgag caaaacagtg agatactgat ggaggaactt
    61981 cgaaaaaaca ataaaacaga tttcaacata atagggaagg taaagacaaa gggtaaatat
    62041 tcaatagaag taatatagac acgagctgcg agctgtgagg attgaggtaa cgctagtatt
    62101 tcgaatctga ttttggaggt aaaaatatga ttgataaaaa gagcatattg agcagcatac
    62161 cctccgttga cgaacttatg aaagataagg agcttgaaag gctgaccgta ctgtattcaa
    62221 gaactgccgt ggtagaaggt ataagggaat acctgaggga atacagaaag catgtcatgt
    62281 ccgccaaaaa agacgaagaa ttggatttag caaatggctc cgatattatg aagggtataa
    62341 tagaaaaaat ccataatatg atgaggaata atcttgtcaa tgttgttaat gcaacagggg
    62401 ttgtgcttca tacaaatttg ggaagatcac ccctcagcag caggctgaag gataccctgt
    62461 ggaatatcgc ttcaaggtat tccaacctgg aaatggactt ggaagcgggc gagaggggct
    62521 cgagatactc tcatgtggaa ggactgatat gcaagctgac gggagcggag gcagcaatgg
    62581 tggtaaacaa caatgctgcg gcggtaatgc ttgttctgag tactgtagcc aaaggccggg
    62641 aagtagtggt gtccagaggg cagcttgttg aaataggagg ctccttcagg gtgcctgacg
    62701 taatgaagca aagcggagcc gccctggttg aggtgggtac taccaataag acacatcttg
    62761 aagactatga aagcgcagtt aatgagaaca ctgcagctct tatgaaggta cataccagta
    62821 attacaggat actgggcttt acgagccagg tggagccgga ggaattggta gttttaggca
    62881 gaaagctggg acttcccgta atagaagacc tgggaagcgg gcttcttatt gatctgacag
    62941 aatatggact gccctacgag ccgacagtgc agcaggctgt caaagcaggc atggatgtgg
    63001 tgaccttcag cggggataaa atgcttggag gccctcaaac cggtataatt gtaggcaaga
    63061 gggaatatat agacaagatg aagaagaatc ctcttacaag agcaatgagg attgacaagc
    63121 ttactttagc tgctcttgaa gggactctga agctttatac tgataaagag cttgctttag
    63181 cagaaattac tgtacttcga atgcttacct atgataacga ggagttgggc aaaaaagcca
    63241 ggcgccttgg tgtaaggctg aaaaaaacca taggcgatat ggcagcaata ggaattgagg
    63301 atgagttttc cgaagtgggc ggaggctcaa tgcctttgca taaaatgcct acaaaggttg
    63361 ttgcaataaa gccaaagaca gtatccctga cgggactgga agaaaagctg agggcttata
    63421 agactccggt gatagcaagg atagtcaggg ataggctgct gcttgacatc aggacaataa
    63481 tggatgatga atataaggtt gtggaaaatg caatgttatg ggcgtttacc cagctatccg
    63541 ggagtaaatt gggggaaggc gagggtcagg cttgaagaat gtgataatag gaacagcagg
    63601 gcatatagac cacggaaaga caactctcat aaaagctttg acgggaaggg ataccgacag
    63661 gctgaaagaa gaaagggaaa gaggaatatc cattgagctg ggctttacgt actttgacct
    63721 tccaagcgga agaagagccg gaattattga cgttcccggc catgagagat ttataaaaaa
    63781 catgcttgca ggtgccggag gaattgatgc agtcatactt gtgatagctg cggatgaggg
    63841 tgtaatgccg cagaccaggg agcatctcaa catactttcg ctgctgcagg taaagaaggg
    63901 cattatagct cttaccaaaa aggacatggt ggatcaggaa tggctggaaa tgataattga
    63961 acaggtcagg gaagagactg caggcacttt ccttgaagac gcaagtataa tacccgtatc
    64021 atcaatcagc ggtgaagggc tgaaggagct tattgcagag atagattcgg tgactgaaat
    64081 cgtggaagag aaggatgctc ataagatatt caggcttccg gcagacaggg ccttcaccat
    64141 aacaggcttc ggtaccgtag tgacaggtac gcttatttcg ggcagcataa atgaggggga
    64201 caggattgag atatatcccc gtaaaaccga gacaagggta cgttccatac aggtgcatga
    64261 aaaaaatgta aagactgcat atgcaggaca gcgggtggct ataaatatag caaatataaa
    64321 gcttgatgaa atccgaaggg gagatatttt agcacagccc gggtgtatgg aacctacgat
    64381 gatgctggat gcaaggttgg aaatattgaa ggatgcggat aagattattg acaataggga
    64441 taggttaaga ttttatcaag gaacctctga gataatgtgt agagtagtgc ttctggacag
    64501 ggaagaattg aagccggggg agagtgcatt cgtgcagctc aggctggagg aggagactgc
    64561 ctgcatgaag ggagaccgtt ttgtaatcag gacatattct ccaatgctta ccataggagg
    64621 agggacggtg ctggaaccca atcctcccaa gagaaagcgc ttcaaagagg aagtaattga
    64681 tgagctgaag attaaagagc agggaaatcc aaccgaggta gttgagaggt atctgataca
    64741 gaacagcgat aaatttcccg aaaagaaaac ggtattaaag gcagcaggga acatgactgc
    64801 cgagctttat gacaaaatag ccgccgagct tctggagaaa aagcttattt acgagttcag
    64861 gcttggtgat gagacttata tacttcatat gaagtatctg agggacgtgg agaccaagct
    64921 tacagagctt ctggagtcat atcacaggag aaatccgctg aaaagcggaa tatcaaagga
    64981 agagcttcga accaaggttt ttgaaggtat gaagccaaga ctggctgata tggttttcgg
    65041 atattttgaa gatggcggaa ttataaggct tgagaatcaa tataccgcac taagcggttt
    65101 taaggtgcaa ttcagcaaac agcaggagca tattagagat actatcctgc aggaatataa
    65161 aaagaaccgg ttcaaccctc ccaggctttc agaactggcg gcagcaaagc gtttgaacat
    65221 aaaccaatgc caggaggtat acattgctct gattgaaatg ggcgagcttg tgaagctgga
    65281 tgaggatatt gctttttcaa agtatgctta taatgaagca gcagagcttt taaggaagta
    65341 tataaaagac aacggttcga tacagcttgg ccaattcagg gatttgctgg gtaccagcag
    65401 aaaatatgct atggcactac tggattattt tgatcagaat aagattacca agcgtattgg
    65461 agataacaga gtattgttct aaaaagagga ctgggtttca gccctcttgg cgtttctgcg
    65521 aagtgaaaat aaccgttgac attaaatggt atcggtgtta tacttatata actggtgatt
    65581 taacgatatt aaaaccattt cctattattt gtcaggagaa tgtataatgt ttgaaagttg
    65641 ttaaatccta ataaataata ggaggtaaaa aaatggctga caagaatttg acttgcaagg
    65701 actgtggaac agaatttgta ttcacagaag gcgagcaggc tttttacaag gaaaagggct
    65761 tcgaaaacga acctcagaga tgccctgact gcagaaaagc aaaaaaagca cagagaaacg
    65821 acagagactt cagaagataa tatggagcaa agcaaaaaag gacttcactc taagagtgtc
    65881 agtccttttt ttatttatca aaaattaagg aatacgctaa tttaaattgg cgggagtatg
    65941 tgggaatcga acccacccat gaggctacca accccaaaca ctggttttga agaccagagg
    66001 gcacaccagc acccatctac ccccatatga agcatgcctt gtacaactac aaatattatc
    66061 ataatgcatt ttaaaaagca atgccaatat aagaggaaaa tcacaaaaaa aaggacacag
    66121 gaacgcggga catagggacg tttcgggaca cggggacgtt tctcctgtcc cactttcagt
    66181 gggacaggag aaacgtcccc gtgtcccgaa atgtccccgt gtcccgaaat gtccccgtgt
    66241 cccgaaatgt ccccgtgtcc cgcccatgtc tcaaacttcc atatgccctt tctattactg
    66301 gcgaaacctg ccgggggatt gagcgatttc ctgcttcatt tgctctatga ttctccttac
    66361 catttcaccg cctaccttgc cgcattgctt tgctgacaga ttacctttat agtcgctgtt
    66421 aaaatctatt ccaaactctg aggcgatgga ctttttcaaa gtatccattg ctacctcggc
    66481 ttcaggtacg agaatctttg agcttcgcct gccggttctt tttcctgaaa tagtcttata
    66541 tgctgtttca gtatttcttg ccatcaaaaa caccgccttt catagtatat gttttgaaca
    66601 aaacactttc ccttattcat atgtcgtggg aagtacatta atttaacaca aaatcatagc
    66661 ctatctgatt ttgcaattat aaatattttt ctcgacattt atagtattct atggattctg
    66721 cttttgcatg agttgaaaat ataggataaa gaaagaaaaa atgaaagcta aagtatgaaa
    66781 ttagtaagca tgtatacagt gaatggcagt gtaagaatgc ttaacagcgt ggttataaaa
    66841 acaccaactg aagcaaataa tggttctgtg tcaaattgtg cggcaaaaat tgcatttgtt
    66901 gcacctgacg gcatggcagc cattaatgtg catatcgata tcaccatggt gttaaagccc
    66961 ataaacctta gaatacaggc agttgccaga ggaattataa taagcctgat tgccgccacc
    67021 agatagattc ttttgtctga aacaagcttt gaagtatcta tttccgcaat tagcactcct
    67081 attagaagca ttgagagggg tgtggtcatt tttccaatca gatcaaatgc tgtctgaagg
    67141 gaatatggca gacttatacg gaatgcaaac attaaaatag caattaaagt tgatattgaa
    67201 ccaacattaa ggagcttttt caggtcgatt ttttcctgtt tgttaaacaa tagtattcca
    67261 taggtccaaa gaaaggcagt aaataccagg tttgaggctg tggcataaaa gactccggtt
    67321 tccgcaaaaa atgctctgac aacgggatat cccataaatc ctacatttcc aaaaactata
    67381 agggaaataa aaacagcgga tgttttctga tcaaagcgca gcccttttac tgtcagcatg
    67441 cacattagta tagtcagtat ataagcaatc gatgtgattg taagaatact gatcagatta
    67501 ggcagcactt cggcagttct tgcaatattg gatgatgaga gtattagcga aggtagtgca
    67561 atctgcatca ggagcactga aattgagttt tgtatgcctt catcgataat tcttgatttt
    67621 tttgcaattg ttccaataaa cattataata aataaaacca gtatttgatt gacagccgta
    67681 ttaaaatcca tataaacctc aatataaaag cagacagtcc tgcttttgtg ataataatct
    67741 attttactat atatattatg taataaagtt gcattataaa aataaatata tcatagattt
    67801 aaatatatgt acaataacgg aaataggggt gcaagggagt gagagggtaa tatggttgat
    67861 aatgacaggg atgattgttt cctaaaggaa attgtggtct taaatgaaat tctcacacct
    67921 cttgtaagct cctacaggtt gtctgtcggg gctgctgaag agtttaacag gatagccctg
    67981 gcgcaccgca aggatgttga ggatgccata gacagggcgg atgatcttgg ccatttagtt
    68041 gatgatgtga ggagaaagct taagaagtgt atgaaaatat atttctcgga actggattac
    68101 aagctggagc atatggagga gatacgggaa aaggctgcaa tgcgcgagaa gcttaattca
    68161 aagcttaaca agatgtctga agaaaaaaaa taaggtgcct gccgcttatt gatgatgcag
    68221 caggcatcct attgtgctat ttagagataa tcagattttt tgcattattc ataaattcct
    68281 ccagcctatc cactctgctg cctccgcctt gggctgttct tgaatttccg ccgcctttac
    68341 cgtcaattat gggaagtact gctttaaaaa gtgcattcat atccatattc acgtcctcgg
    68401 aacggctgaa tatgacttgt gcattttcat ttttgcaagc cagaagggca acagtccccg
    68461 gtacctttgt cagatactgc gcaagaagct ttacatcgtt tatgcttctg ttgtctaaga
    68521 ctctgcttat cagcctgata cctgatacta ccggacattc cttgatagta ttgtcagctt
    68581 cgattctaat caactcctgg gtggatgcgg aaagctgctt ttcagtattc cttaagtcaa
    68641 acagcaactt gtcaacggct tcaacaattt cagaatccct ggccgacagt ttttcacaaa
    68701 gagcccggat gattgaatta tacaatccat agtccttcaa cgctctgttt ccgcatacaa
    68761 acgcaaattt gatgcctcct ttgcattttt cccagttttt tatctttatc attcccactt
    68821 cgccggttgt cctcacatgg gtcccgccgc aaggagagta gtctgtttct ttgacttcaa
    68881 ctattctgat attttcggta accttcggca tttttcttaa aggcaggctg ttcagctctt
    68941 caccgcttac aattctcgct attaccggca ggttgctgta tattatatca tttgccatat
    69001 tctcaatgcg ctcggcatca tcaatgctga agctgtcgat gttgatttct atgcttacta
    69061 tatccttccc catatggaag ctgtcggtgc ttccggttaa aagcttttca aagcaagccg
    69121 acaatatgtg ctggccggta tgctgctgca tgaagtcaaa gcgtcttgcc caatcgacag
    69181 agccttttac ttctgttgac aggagtttat tatcaacaat atggagcagc ttcccgtcct
    69241 tttcaattac ctccgttact ttttcatcat tcaaatatcc cgtatcccag ggctgcccgc
    69301 ctccggccgg ataaaatgcg gtccggtcca gtacaattcc gtatttgtct ccataagtca
    69361 gtgtttcaat aacttcagcc cggaactccg taagataggg gtcgtcatta tataatcgtt
    69421 cagttctcat atttgcctcc tttttatgtt tagcatgccc gcaagtccag ttcaataatg
    69481 ccttttgctt tttatcgtat gggaaagtat gctttataga ttaatccaaa agcatttctg
    69541 cttttgtcaa aacatccctt gcgcttcctt ctatcactaa gtcaaattga ctgctgctcc
    69601 tgcccgtatc taagttgata taggccttct tccctttgca cagctccggc agttggttta
    69661 ccggatatac ttgaagactt gttcctacga caaccgcaag atcagcttta ctgattgaat
    69721 gtgttgcatt atccaatgct tcatatggaa gggattctcc gaaaagcaca acatttggcc
    69781 tgagtttccc tttgcatttt gcacacggct tcttttccaa gaattccttt aattctgctg
    69841 cattgctgca gtcctcgcat ctgacagtcc tgatatttcc gtgcaattca tatacattgc
    69901 ggcttccggc ctcctgatga aaccggtcaa cattttgtgt tgcaatgcaa tgtaccaggc
    69961 cccttttttc ccattcagcc aatactttat gaccttcatg gggcttacag ccttcaagtg
    70021 tgtttatccg catgatatag aagtctctga acagctcata ccggttttcc aatgcatatc
    70081 ttgaagcaac ggttgcagga tcaatatttc tccaccaccc gcctggcgaa cggaagtccg
    70141 gaatgccgct ttcggttgac atgccggcac cggtcaaaac cacagtgtat tttgactgct
    70201 tcatccatga tgcaagcagg cttaatttac tgctatccat atgaagaacc tcctctatta
    70261 cttatacggc tttgggatta ttacctcaaa cattaacctg tactttaatt atatatgctt
    70321 ttgtaataaa gtaaaccaaa aaacctgcaa agtccaaagc cttgcaggcg ttgaatggct
    70381 gctccttttt aatgccctaa agacatctgt tttgctctgt cgcatttatt ggtacaaatc
    70441 atattatgta ttgaagggag gggtaaatat atgaacatta gcgaaacatt agtggtgccg
    70501 attatagttt cattaacaca gcttgccaaa gggcttgggc tgcccaagaa gttttgtgct
    70561 cttgcagctg ttgttattgg agttattata ggtgtttttt ttcttgaacc tctgtgtata
    70621 aaagcagggg ttttcaaggg catagtatat gggctttctg catcagggct ttattccggt
    70681 acaaaaaata ccattgaaca ggtaaacggc aaaaaagtaa agaaaaagaa aaaatagaga
    70741 attcaaatta cagaagtata tatttatctt tctgaggtta aattaaactc aggaggtgaa
    70801 ttgcatggga aataacacac ctaagaatga tcagcaaaga aggtcgacag gtacaggagt
    70861 aattggtttc attgtcagat tcgtagttgc cgcaattgta cttatggtta cagcttattt
    70921 tgttcccggc ttcagaatga gaggaggctt cactacggca attattgctg ccctggtaat
    70981 agcggcagcg gattttctta tcgaaaagct tttcaaattt gatgcgtctc ctttaggcag
    71041 aggcatctca ggcttcctgg tttcggcggc ggtaatatat tttactcaat acctggtgca
    71101 gggaatggaa gtaagtttat tgggggcagt aataggggca ttggtaatcg gccttgtcga
    71161 tacagtgctg cccaccagat ttatgtagta ttgcaaatag ctgctaatgg cagctatttt
    71221 atttttttat atcgagtagt aaaaaatgta aataatagaa ggatattcaa agttttggta
    71281 gaaattatac aaatccacaa aattttgaaa cgggcgtgag gttatggcga gctttgcttt
    71341 ttccttcttt ttcggtatat tgactgcgag tattatttta cccgatattc cgattatatt
    71401 gggtggtttt ttattgtcgg gggcggcagt gcttataaac agaaaaaaca aaccaaatct
    71461 caaggcagta tttatgcttt tagtttttct tgtgggcatg gcggattaca gtcttgccta
    71521 ccgagcagag agcaggctgg acgcatttga cggaaaaact gcagtctatg aatgcctgat
    71581 tacggataat cccgtggaaa gggggaaata tctgcagtat ccggccagaa gcatttcggt
    71641 tcaatatgag ggaaagcact acaatttctc cgagaaggtt ttcttgaaga ttgatgcaga
    71701 taccgtattc aagttcgggg ataagctgtc catccggggc caatgttccg atatagccgg
    71761 aataaggaat cctggagact tcgactttaa attgtattat aagaacaaag ggatagccaa
    71821 gcagatagca gccgacagag cggccctgct gaaggagaat agtgcaggag tgttcaaggc
    71881 aatgctgtat tattcaaagg agaaggtcag gagtattatt aatgaagcct tgcctcagga
    71941 ggaggcggca gtactcaccg ggattattac cggtgataag gccgatatgg atgaggatat
    72001 gaaagaagca tatatgaaaa cagggctttc ccacatactt tcggtttccg gccttcatgt
    72061 cggctttctg atgatgcttc ttacatattt gttaatgcct ttcaaactgg ataagaggct
    72121 tcaaggagct gtcaccctat taataacagt atattatgta cttttgatag gagccccttt
    72181 tccctctgtg agggcggtta ttatgcttgc agtcctgatg gccggcaagg cggcggggcg
    72241 ggagtatgac cttttggcgt cagtatcctt tgcatttatg ataatgcttg tatttaaacc
    72301 cctggcaata catgatacgg ggtttatgat atcctttgcc gcgatgtatt ccattgccct
    72361 gctttatccg gcagtttacg gcatattgcg gtgcatgcct ggggtaataa ggaatccggc
    72421 ggctttgtcc atgtcggtat ggcttgggct tgctcccgtt ctggcacact attttaacta
    72481 tatttccatt ataagcatca taataaatat tgctgcaata cctctgtcct tcattattac
    72541 agttacgggc tttatagggg tgtttgcggg aattgtatca aagactttgg cactgtatat
    72601 attttctgtg gattactgtt ttattaacct tctttccttc atgattcgaa aagctgccga
    72661 gcttcccgcc gcaggctttt acatcccaag gctgccaata tatattcatg ctttgtacta
    72721 tggaggtatc ggactgttca taggattttg caagctggcc tttttcaggg tatatatgcg
    72781 ccggcttgct ttatcatatc tgcttgcagc tgttattgca atttctgtct acaacctgcc
    72841 ctcggaaaaa ctgagaatgg tattctttga tgtggggcaa ggggacagct gctgtatcat
    72901 tacacctcag aaaaaggcag tactgattga cgggggaggc tcttcaagga atggggatta
    72961 ttactatgac gtaggaggga agataacttt tccggcactt ttgcaccagg gaatatggag
    73021 tatcgatacc gttatcgtat cccatctgca tgatgaccat atggaggggc ttctgaaagt
    73081 tatggagggt tataagataa aaaatataat cctgccccgg gtttcggcgg gtacggatga
    73141 aatatcggga agcagcggtg cgctgcttga tatatgcaga aacaagggga taaaaatcca
    73201 taggctgggc tcgggagatc aaataaacct tgggggtgga gtaaagattg attttttgca
    73261 ccccggaaaa gctgcaaaag ctgatgagaa tgaaaactct ctggtggggg ttctgtatta
    73321 cggagatttc agtgcacttt ttacagggga cataggaaag gagacggaag gcctgcttca
    73381 agccgaagct ttaaagtccg ttgtgatgaa ggtgcctcac cacggcagcg gaagatcctc
    73441 ctcggaagaa tttcttgaga aagtcaggcc aagggtcagt gtgatatcgg taggcagcaa
    73501 caattatggg catccttctc cggataccct gaaaagactg gccggcatcg gaagccttgt
    73561 atacagaacg gacgggaacg gaggggtaac cataataaca gacggagaaa gtatgaaggt
    73621 aaaaactgtg aagcagtgac agttgatagc ttatatatta taattaaata aatactgttg
    73681 aaacggcatg gtgatgaaat tatgggctac aggcagctta tgaacgatat taaaaacaac
    73741 tgcatcaaac cggtatacat catattcggc aatgagactt atctcataga caaaggcaga
    73801 gaagccctga aaaaagcggt ggtgacctcc tttccggagc ttaattatac ccggctggag
    73861 ggggagaagc ttgaggccga tgagctatca gccgcttgca ggacttttcc ctttggaact
    73921 gaaaagagat tagtagaggt gagaaatcct gaaatactga ggagcaaggg caggggcagg
    73981 gaggaagatg aggatgagga agagaatgaa gatgaaaagg cttctgctcc ggggaaggat
    74041 gcacagccct ttattgaagt gctgcaaaac cttgatgata ccgtttgcct tttgctgctt
    74101 tcctatggaa gtattaataa aaaaagaaaa aaacttctcg atgaagtgaa aaagcatgga
    74161 gcggttttgg aattcaacag agtagaaagg gatgagctgg ctcagtggat aaagaaaacc
    74221 cttggaaaga gcggcaagaa tatcggcccc agagaactga atcatttcat cagcagtacc
    74281 ggttatcttg acaaaaatgg cagcaaaacc ctgtatgatt tagagaatga aataaaaaag
    74341 ctgacaggtt ttatgggaaa ttcaacggag gtcactatag agcatattga agcaattgtt
    74401 ccaaggaaca tagaaaatga catattcaag cttattaatg catgctatga aaaagacaca
    74461 ggccgcagcc tcaggatatt caatgatttg ctgcttgagg gggagaccac tttaggagta
    74521 ttggctctcc tgtcaagtca ggtaaagaac atgctgaaag tatatgagct ttatgagaaa
    74581 aaatataaca ggagaggtat atcggaaaag cttaagcttc atgaatatac ggtgaagctt
    74641 tgcatacagt acggttcatc aataaaaagg caggcactgc agtctgcttt cagaaagtgc
    74701 ctggatactg atctgagtat aaagtcagga ggtatgggag accggctggc gatggagatg
    74761 ctgcttgcgg gattatttga gtaatataaa tagggtgccg ctaactgcat attaaaaagt
    74821 tttgcggcac ccgaaatttt tgttcgtata ctaagctata tcctttatct tttttgataa
    74881 tctggatttt ttccttgctg ctgtattctt atgaagtgta cctttggaaa ctgctttgtc
    74941 aataagacta gcaaccttcc tgtacaattc tttagcttcg ctcatatttc cgttcttaat
    75001 ggaatcttca aaccttctta ttgaagtttt caaggaggaa acaattattc tatttcttct
    75061 tgtcttgaaa tttgccactt ttattctttt taaagctgat tttatatttg ccaaaccctt
    75121 cacctccttc aaaattcaat tgacaaaagg tattctatca tattagccaa aaaaatgcaa
    75181 gtgcattgta ttgagctttt ttgggcatgt tttatatatt atttatataa gtttacccgc
    75241 tttaaaatta attatgaaga actgcatttt gtataaaaac atcaggtaag gcgcaaactt
    75301 caattattaa ttaatgaaaa acactttaca gatttttgga gggaatgccg tggccggaat
    75361 aagaacagac ttagctattg aagcaaggga attttataac aagcaggaaa acaggcaaga
    75421 tatcccggga gtggttgtgg atgtggacaa agatgagagt tataccgtaa ccagggttaa
    75481 ggttgttgaa gatgtcggtg caagaatcat aggaaagccc aaagggaatt atattaccat
    75541 tgaagtgccc aaactgaggg aaaatgacag ggagctccag gaagaagtgg gaaaggttct
    75601 tgcggaagaa gtagcaagat tgatgagcct caatgaccaa tccgtgataa tggtggtagg
    75661 gctgggaaac tggaatgtaa ctcccgacgc actggggccg agggtagtgg agcatctgct
    75721 ggtaacacgc catattaagg aatacgttcc cgatcagatc gacgagggag taaggtcagt
    75781 ttgtgctatt gcccccgggg tgcttgggat tacagggata gagaccagtg aaataataag
    75841 gggcatagtg gataaagtga atcctgacct ggtaatagcc attgatgcct tagcttcaag
    75901 gaaaatggac aggataagca caacaatcca gattgcggat acaggcataa acccgggatc
    75961 cggagtcggg aacaacagga aggaacttag ccgggagagc atgggggtgc ctgtcatagc
    76021 catcggagtt ccgacagttg tagatgccgc aacaatggcc agcgatacca tagatatggt
    76081 gattgacact cttataaacg aatctcctga aagcggagaa ttctacaata tgcttaagag
    76141 catggataaa acagagaagc ataagctgat agacgaggtg atttccccgt atataggtaa
    76201 ccttatggtc actccaaagg aaattgacag gcttatagac gatatagcaa tagtgatagc
    76261 caacgggctg aatattgcac tgcacccgaa cgtagatcag gatgaaatga acaggtatct
    76321 gaactaaaaa agtgcgtcgt gagacgcatt ttttctagtg tatggcatat atttgtatac
    76381 agacaggtcc aatttttaat cgggttcaaa agacgggcgg aaaagaggtg gagtgtttga
    76441 acaagaaatg gctgtttgcg ctttttctgg cactggcagt tgcagcatcg cttttatttc
    76501 tgaaatggtt atattcacct gacccatatt taagcaaata caatatcagc tcaaccggct
    76561 ctgcagaggt tttcaattat gacattaaaa agaggctaaa gcttgaagaa tactccaaca
    76621 acttctacat acagctgtta aatactgtaa tgacaggagc caaggcaaat tatgagattg
    76681 aatacggctg ccctatgccc gggcttcttg agtctgctgc aagcagcatc accgacgggc
    76741 ttcataccgg cgccggcaat cccataactt acctgaattt tgtctttaca gctttcagtc
    76801 aatatgatcc gtcgctgttt tcatatgccg aaaccggcag cagtgcattt cccaatatta
    76861 ccgattttga aaatgcgcct cagggagcta tttatttcag cgaggaagat gaatataaag
    76921 ctgagatacc cgcagaaacc ccggcatcac aaattgtcga ggaagctgtc gccatccccg
    76981 gcaaaatagc attgaacagc aaatctccgc agatactcgt ttatcattcg cattccacag
    77041 aaagctacat gcctaatact gcagggaatt atcataccca gaatgacaag tacaatgtta
    77101 tagccgtagg aagcatattt gccaaagagc tgcaggataa atacaaatat aaggttctcc
    77161 atgacaagac caagcatgat aaagagtcct atgcatattc ttatgcaaat tcccttcaaa
    77221 ctataaagaa cagcatcaat aaatacaatt ccataaaagt aatcctggat ttgcacagag
    77281 atgcctttga tgcggagaaa gccacatttg ctgagaaaac agccaaaaaa tccgaataca
    77341 ccacctcaat aaacgggaaa agggctgcaa ggataatgct tgtaatagca aaagagaatc
    77401 caaactacgc agagctggaa aagtttgcag tatatataaa gaaaaagatg gataaactat
    77461 atcctggatt attctacaaa atcgatataa aaagcagagg caagtacaac ctctatttca
    77521 gcaatcactc catgctggta gaggtaggat gtatgcttaa tactattgag gaagcccaat
    77581 attccgcaga gctttttgca agggttatgg gggaagtaat aaacgacctg aaggaataga
    77641 tgcagatgag caagagagtt gataaaaaaa aacagaggat ggaagcattg aaaaataagc
    77701 tgaagaaggc cctggggata ttcttctgca taattctgct gattctttca gtcagcattg
    77761 cagatatgtc agccagaaga atgatgatgt gcaatgacga taagtacgca atagcagttt
    77821 ctttgcagga ggacagcctg ctgagggtag acattgccgg agaaaagctt atgataaata
    77881 tagaacctgc cgtcaggatt ttcaattata tttacgacaa ttccacgagg cactgtgaca
    77941 gtattgtgaa aagcataaaa gcaaaactta atgaatgatt ttattttata aagaggacat
    78001 ggggaggaca cggggacgtt tcgcgtgtcc cactggaagt gggacagggg aaacgtcccc
    78061 gtgtccccgt cccccgtccc ccgtgtcctc ttattcttct gttgtgcata aacctatttc
    78121 attttttgga atccggtgta tatagtatat atttattgtt aaaaactaat atgctataat
    78181 atgcgtaaat gtataaaact catttgatac aatcttaaag atttaagggg cacaaaggag
    78241 gacgccatat atggcagggg acaggcaaaa gcatataaga aatttctgca taatagcaca
    78301 tatagatcat ggaaaatcga ctctggccga caggcttata gaaaaaacag gggttatttc
    78361 cgccagggat atggaggaac aggtacttga caatatggcc cttgagaggg agagaggaat
    78421 caccataaag cttcaatcgg tcaagctgga gtatacggct caggatggac aggaatatgt
    78481 attgaatctt atagatactc cgggacacgt ggattttacc tatgaagtct ccaggagcct
    78541 ggctgcctgc gaaggggctg tgcttgtggt agatgccacc cagggtattg aggcgcagac
    78601 actggctaat gtatatctgg cccttgataa taatctggag ctgtatcctg taataaataa
    78661 aatcgatctt cccagcgcag acccggagtt cgtaaaaaag gaaatcgaag atataatcgg
    78721 ccttgatgct tcagatgctg cattgatatc tgccaaggaa ggactgaaca ttgaagaagt
    78781 acttgaagga atagtgaagt atatacctca tccggccgga gatgaggata agcctttgaa
    78841 agctttgatt tttgattcct tctatgacag ctataaaggt gttattgcct atataagagt
    78901 agtggacggg gtactgaagc cgggcatgaa tatgcgaatg atgtcaaaca acaaggagta
    78961 cgaagtggtt gaggcaggca cactgaagcc ctggctgaat cctaccggcc tgcttatggc
    79021 aggagatgta ggctatgtaa cagccagtat aaagaatgta aaggatgtcc atgtgggaga
    79081 taccattacc gatgcggata acccctgcaa ggaagcacta ccgggctata ggaaggttac
    79141 ttcaatggta tactgcggta tttatccagc agacggagca gattacgaga accttaaaga
    79201 tgctctggaa aagctgcagc ttaatgatgc tgcactgctt tttgaaccgg agacatcggt
    79261 agcactgggc tttggcttca gatgcggctt cctgggattg ctgcacatgg aaataatcca
    79321 ggagaggctg gaaagggaat ataatcttga ccttgttacc accgcaccaa gtgttatata
    79381 tcatattacc aagaccaacg gagaaaagct tgaggtttcc aatccgacaa acctgccagt
    79441 tccccaagag atagaatata tggaggaacc cattgtgaag gccactataa tgacacctac
    79501 agaatatgta ggagcgatta tggagctttg ccagaaccgc aggggcacct tcagggatat
    79561 gcagtatatg gaggaaaaga gagccatact taattatgat ttaccgctga atgagataat
    79621 ttatgacttt tttgacgcat taaaatccag gacaaagggc tatgcttcct ttgactatga
    79681 gctgaaggga tatgaaaggt cagatcttgt taagctggat atactgctta atacagaagc
    79741 ggttgatgca ctttccttca tagtacataa agataaggca tatgccaggg gaaggtctat
    79801 ggctgaaaag ctcaaggagg taattccgag acagatgttt gagattccca tacaagcggc
    79861 catcggaaca aaaattattg ccagggaaac tgtaaaggca ttgcgtaagg atgtgcttgc
    79921 caagtgctat ggcggagata tatcaagaaa gaaaaagctt cttgaaaagc aaaaggaagg
    79981 aaagaggaga atgaggcagg ttggcagcgt ggagctgccc caggaagcct tcatgtcggt
    80041 cctgaaactg gatgattaaa agtgggtgaa aaaatgaagg aagtgggttt gtacatccat
    80101 atacctttct gcaggagcaa gtgcctgtac tgcgatttta attcctaccc gtgcagggaa
    80161 gatgatgcgc cggagtatac agaagcgctt ctcagggagc ttcatgcata tcaggaaagg
    80221 tacaattttg tttacaagac agtattcatc ggtgggggaa ctcccaccgt tattaattgc
    80281 aggcttatag gtgaaataat ggataagatt gcaccccgga taaaagcagg agcggagatc
    80341 agcatggaat ccaatccggg aactgtaacc tttgatactc tgaagtacta tagaagcctt
    80401 gggataaaaa ggctcagcat aggcctgcag gcatggcagg cagaactgct gggtgagctg
    80461 ggaaggattc acactactga ggattttctg aatgcctata attgggcaag agaatccgga
    80521 tttgacaata taaataccga tttaattttt gcacttccca atcagaccac cgatatgtgg
    80581 aaagaaaccc tggataaggt cgtaagctta ggcgtggatc atatttcctg ctacagcctt
    80641 atactggaag agggcacagg attatacagg ttgtacaaag aaggcaaatt gcagttgcca
    80701 gacgaggagt tggacaggga aatgtacaga atcgcagtgg atacattggg ctccaacggc
    80761 tacctccaat atgagatatc caactttgcc aggaagaaca tggaatgcag gcataatctt
    80821 gtgtattgga gaaatgagga atatcttggc gtcggagccg gaagccactc caagctggag
    80881 gggaaaagat tctggaatta taaagacatt aaccggtata tcagcctggc cgctgagggg
    80941 aaactgccgg tggaagggca ggaggaatta tccgccgatg aggacatgtg ggaaacgata
    81001 tttctggcac tgcggctcaa tgaagggttg gatatggcgg gctttaacag caaatacggg
    81061 gttgacttca gaacgcgata cggcagccag cttaagaagc ttgcggcaca aggccttgtg
    81121 tttattgaat cggacaggct gaagctgaca aagaaaggga tagacctatc taattctgtt
    81181 tttgtggaat tcgcataagg agaagaaggc aacacattct tcgggtacga ggtgcgaggc
    81241 gcgaggttag aggtttaggg cagggctttg ggagctatgg taacactgaa aaaacgaaaa
    81301 accgctaaat gcacggcggg ataaatattc cggtaaatag gtaattatcg ataatgatgt
    81361 aagtatcgaa accttcttat gcaactatat aacatatata aatgttatag tgtaatcgcg
    81421 ccgcattctt cgggcaattg gtgggtggtt tgaataatgc gacacatttg aagtttgtag
    81481 tcatcctaca gagcactagc cttgtataac agcgagcact tattggcttt ggcaacagaa
    81541 tcgagagaag atacgcggta aaatccgtgg gatttacgtg aggtaaatcc agcacatacc
    81601 aacaggaggt tgggtttatg tgcgtcaggg ttttgcaagt atcaagctct gattctgtcc
    81661 aaagctaata tcgtgtgagt cggtatacaa ggctattgct ctgggagtat gactgcaagc
    81721 ttatatttac gacgtatcat tcttctatta agcagaaaaa agttgtaaaa cctattgaca
    81781 aaaatatgtg acaatgttat tttaaagaca gaattagcac tcaatacggt agagtgctaa
    81841 caagggcggt gatattgatg gatatgggaa acaggaaaag acttatactg caagctatta
    81901 ttgaagatta tataaactcc gcagaacctg tcggatcaag gacaatatcc aagaagtacc
    81961 tgacgggtac cagtcctgca acaataagaa acgagatgtc tgatcttgag gatatgggat
    82021 atattgaaca accccataca tcagccggaa gaattccctc agacaaaggc tacagattat
    82081 acgtagataa gattatgaag caggataaaa ttaatgaggc tcaaagggaa atgataaaga
    82141 gggagttcgt tgaatccctg ggagagattg accggcttat aaagcatgcc tcaaagctgc
    82201 tctcacagct gactcaatat acatccatag tgatggctcc tcagtttcgc agaaccagca
    82261 ttaagcaggt gcagcttctc agaatagaca gagcggtagt attggcggta atagtaacga
    82321 atgcggggat aataaagaac agtgttctaa gattgaagca ggatatacag ccggatgccc
    82381 tcatcagaat aaccagtatg attaatgaca agatggctgg gctggatgta gaggaaatag
    82441 aaaaatttga ctttcaggac atgctgaaca attctcttgg acacagtgaa ataattgaac
    82501 aagtggtacc tgagcttata cagacattat tttattccga cacttcagaa gtgtaccatg
    82561 atggggtttc caacattttg aatcttcctg aatacagcga tatcaacaaa gcaagggatt
    82621 tcctcaatat aatggaagaa agggaactgc tgtttaaagt gctgaatgat gttcgtgagg
    82681 atgtaagtgt aagcataggc aaggaaaaca agcttgagca gttcaaggaa tgcagcctta
    82741 taacggcaac ttataagatt aataacaagg ttatcggctc ggttgggata attggcccca
    82801 caaggatgga atattccaaa gccatttctg ttgttgagtg tatgacaaat aacctcagcg
    82861 agatattgac gagcattctc aagaggtaat cttaaatata aaacttaaag gtggtcagat
    82921 tgatgaagca taaaaaacaa aaagaagagg aaaagccgca ggttaataat acgattgaag
    82981 agaatacggc ggaagaagct tctgcacaaa caggagaagc agttcctgat caggcggcgg
    83041 agactgataa caaagaaatt cttgaactga aaaagcagtc tgaggaatac tataacaggc
    83101 ttctaagaat gcaggcggat tttgaaaact ataaaaagag gatagcaagg gaaagggaag
    83161 atatgtacaa taacgtcctt gaaactataa ccgcacagct gctgccgatt gtggacaata
    83221 tggaaagggc agtggaagcg ttcaataagg ataagctgga tgaacaatac atctcaggag
    83281 tagaaatggt ctgcaatcag atcatagata ttcttaaaaa gaatggcgtc aaagaaattg
    83341 aagccatgga taaggaattt gaccccaatc ttcaccatgc tgtaatgaag gtgcccggtg
    83401 aggatgagga tgaaaataaa ataaaggaag tatttcaaaa aggatacatt ctgggaaaca
    83461 aagtaatcag gccttcactt gtaaaagtgt cggtgaagca ataagtgcta aaaaataagg
    83521 aggaagcaat aaatggcaaa agtaataggt attgacctgg gaactacaaa ctcttgtgtc
    83581 gcagttatgg aaggcggaga gccggtagta attccaaatg ctgaaggcgg aagaacaacg
    83641 ccctcggttg taggattttc aaaaacagga gaaaggcttg caggccaggt tgcaaaaagg
    83701 caggcagtta caaatccgga gagaactgta atatcgataa aaagggaaat gggatctaat
    83761 ttcaaggtga atattgatga taaaagctat acccctcagg aaatatccgc aatgattctc
    83821 acaaagctta aacaggatgc ggaagcttat ctgggcgaga aggttacaca ggctgttata
    83881 accgtacctg catatttcag cgacagccag aggcaggcaa caaaggatgc aggcaagata
    83941 gccggccttg aagtgctcag gataatcaat gagccgacgg cggcatccct ggcatatggc
    84001 cttgacaaag aagaaaatca gaaaatactg gtgttcgacc tgggaggggg caccttcgat
    84061 gtatcgatac tggagcttgg agacggggtg tttgaagtat tggcaacaaa cggcaacaac
    84121 aggcttggcg gtgacgactt cgaccagaga gtagtaaatt atcttgccga cgagttcaag
    84181 aaggataacg gaattgacct gcgcaatgac agaatggcaa tgcagaggct tatggaggct
    84241 gctgaaaagg cgaagattga gctttcggga gtgcttcaga caaatataaa tctaccgttt
    84301 attactgctg atgcaagcgg accgaagcat cttgacgtta cccttacaag ggctaagttc
    84361 gacgagctta cccacgatct ggtggagatg acaatggagc ctgcaaggaa agccctggca
    84421 gacgcgggcc tcacagcaga caagttgagc aaagtaatat tggtaggcgg ttcaattaga
    84481 atacctgcgg ttcaggaagc cgtgaagaag cttacaggaa aggaacctta caaggggata
    84541 aaccctgacg aatgcgttgc aataggcgct gcgatacagg ccgcagtgct gacaggagaa
    84601 gtcaaggatg tactgcttct tgatgttact cctctttcac ttggaataga gacattaggc
    84661 ggagtattca caaagcttat agacagaaat acaaccatac cgacaaaaaa gagccagata
    84721 ttctctacag ccgctgatgg gcagaacagc gttgaaatac acgtactgca gggcgaaagg
    84781 gaaatggcag cttacaacaa gacattggga aggttccagc ttagcgggat acctccggct
    84841 ccaaggggag tgcctcagat agaggtaacc tttgatatag atgctaacgg tatcgtacat
    84901 gtatctgcaa aagacctggg taccggaaat gagcagaagg tgacgattac agcaagcacc
    84961 aatctttctg acagcgatat cgaagcagcg gtaaaagaag cagaaaagtt tgccgcagaa
    85021 gataaaaaga gaaaagaaga ggttgaagta aggaataatg cagacagttt ggtatatcag
    85081 accgaaaaga accttaagga ccttggagac aaacttggca gtgaagacaa agcaagaatt
    85141 gaagccgaat tgaataatgt caaggaggcc ttgaagggaa cagacacgga agcaataaaa
    85201 gctgctgctg aggaacttac aaaggtattc tatgacatat cggggaaaat atatcaggct
    85261 aatgcggggg cacaggggcc tgccggggaa gctccgcagg atgacaatac tgtaaatgca
    85321 gattataagg ttgaagacga caagtaatta gtgatataat tattgcggtt caaaggtact
    85381 gagcaggata ctttatagtt tcctgctcag ttatgataat tatgggtacg agaatggcgt
    85441 cattattcat agactggttt atgtaaaggt ggagaagaaa tggcatcaaa aagagattat
    85501 tatgaagtat tgggcataga taagtcagct tctgaagaag atataaaaaa agcatacaga
    85561 aagcttgcaa agaaatacca tcctgatatg aaccagggtg acaaaagtgc ggaaagtaaa
    85621 tttaaagaac tgaatgaagc ttatgaggta ttgagcgata aggacaaaaa agccagatac
    85681 gaccaattcg gccatgcggg agttgatccg aatggctttg gcggtgcggg aggttttggc
    85741 ggcttcggag gctttggcgg ctttggcgga tcaaccggta ccggcggctt tgaagacata
    85801 ttcgacatgt ttttcggggg caccggaggc tttggcagca cgggaagcgc cgggaggaaa
    85861 accggccctc agaagggtac ggatcttaag tacgagcttg agatagagtt tgaagaagca
    85921 gcttttggag taaaaaagga tatacatgta acaaggaatg agacctgtaa tgactgcaaa
    85981 ggcacgggag caaaagccgg aaccagcccc gagacctgca aggcatgcgg aggaagcggc
    86041 gaagtaaggt ttacccagag cactattttt ggaagagttg tgaatgtaag gacatgcgac
    86101 gagtgccatg gggaaggcaa ggtcattaag gagccttgcc cccagtgtaa gggcaggggc
    86161 agcataagaa gaagcagaaa gattactatt gatatacctg ccggtgtcga taccggttct
    86221 gttatgcctc tcagaggaga aggagaaccg ggtttaagag ggggttccaa cggcgatctg
    86281 tatatttaca tgagagtgaa accccataag ctgtttaaga gagatggcat aaacctatac
    86341 agcgaaatgc ctatttcctt tactcaggca gccctggggg atgagataga agtgaatacc
    86401 cttgagggaa agatgaagta tacaataccg gaaggtactc agacgggtac tactttcagg
    86461 ataaaaaata agggcatacc aagcctgaag agcaaaatga gaggagatct ctacttcaca
    86521 gtcaaggtaa atgtaccaaa gaagctcagc gagaagcaaa aggagcttct aagacagtac
    86581 gctgcgataa gcggagacga tattgaggac caggggaaat ccttctttga caaggtcaag
    86641 gatgcttttg ggaaataacg aaaagctcgg tgatgccggg cttttctgtg tatccggcac
    86701 tttaacggca ggattttgtg catatattgt aaatatttat cttattatat ataatgtaaa
    86761 gatgacgaat tgtaacatga agtacaagcc ttgaatattt tattgtaaga ggatgataga
    86821 ttgaagtacg tagaactgca ggtaataaca actaccgagg catcggatgc ggtatccgag
    86881 atattgtata gtgagggagc aagcggggtg cttatagagg atccgaatga tttcaatgca
    86941 ttgaataaga atgaaaaaag ctgggattat attgaaga
//
LOCUS       DAOQUR010000004        85609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_329_length_85609_cov_9.211083, whole genome shotgun sequence.
ACCESSION   DAOQUR010000004 DAOQUR010000000
VERSION     DAOQUR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 85609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85609
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_329_length_85609_cov_9.211083"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..712
                     /locus_tag="PKI50_01515"
     CDS             137..712
                     /locus_tag="PKI50_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT02428.1"
                     /translation="MKAVILNGGKENDLSITEVLGTIVKELEATGADIRTFDLRDMNI
                     SRCLGCFNCWVKTPGVCIIDDDGRMIAKEAVQSDLVIFLTPIVFGGYSPELKMALDRL
                     IPIVSPFFTKIQGEVHHKPRYKHYPSLVGIGILPENDQNKEKIFKTLVNKNAVNMHNP
                     SWAACVFSGSQPAEQIRQQIKESLAKVGVIE"
     gene            709..1380
                     /locus_tag="PKI50_01520"
     CDS             709..1380
                     /locus_tag="PKI50_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNT02429.1"
                     /translation="MNPKKAVLFIGSPKERSASEALGAYLLGKLKEKGLSTDKIKIDK
                     LVSTERGQLELFDAVNQADIIIFSCPLYVDSPPAPVIKAMELITENRKGKGKENGKKF
                     LAISNCGFPEAFHNHTALEIYKEFASEAGFKWLGGLALGGGPAVQSSELTKIGGMAKN
                     IMRSLDTAANDIVEKEALSHEAVELIARPLVPAWMYLFIAGFGWKIQAKKHGVSRSIN
                     DCPYE"
     gene            1514..1645
                     /locus_tag="PKI50_01525"
     CDS             1514..1645
                     /locus_tag="PKI50_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02430.1"
                     /translation="MENAFNSFSEITGSARQELALENICKHLDDGGTFVCTLYNTDY"
     gene            1687..1953
                     /locus_tag="PKI50_01530"
     CDS             1687..1953
                     /locus_tag="PKI50_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02431.1"
                     /translation="MISYYNVYNGKTKNITGIQFYEIYDKDNKLKEKRCLDICFSLIS
                     KEDFIEMASKAGFKIKEIFGDYNFSPFSEDSMFMNFILNKEQKG"
     gene            1958..2362
                     /gene="ndk"
                     /locus_tag="PKI50_01535"
     CDS             1958..2362
                     /gene="ndk"
                     /locus_tag="PKI50_01535"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNT02432.1"
                     /translation="MERTLVIIKPDVMERKLMGKIISIYEQEGLHISSIKILKPSVET
                     AESHYSEHKGKSFFIRLIEYITRGEVCVLIAEGDNAIDRARKINGATDPAEADKGSIR
                     GRYGVSKTENSVHASDSAESADREISIWFPEL"
     gene            2387..2803
                     /locus_tag="PKI50_01540"
     CDS             2387..2803
                     /locus_tag="PKI50_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNT02433.1"
                     /translation="MKSFRKELYFKLPVRRGYVNITNQIQACINESGIKEGFVLVNPM
                     HITASVFINDDESGLHQDFEKWLEALAPEKPHSQYRHNGFEDNADAHLKRQLMGREVV
                     AAVTEGKLDFGTWEQIFYGEYDGKREKRVLVKIIGE"
     gene            2887..3366
                     /locus_tag="PKI50_01545"
     CDS             2887..3366
                     /locus_tag="PKI50_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT02434.1"
                     /translation="MKTLIVYDSVFGNTEKIAEAIGDSLAELTGTEVEKVSEAKLEQL
                     QGTDLLIVGSPTRAFKPTKAIVDFLIQIPSNGLKGINVAAFDTRISTEDVNSRILNGF
                     VKIFGYAAKPIADKLQKKGGNLVAPPEGFFVKDNEGPLKEGELERAAKWAKQVMKGE"
     gene            3369..4139
                     /locus_tag="PKI50_01550"
     CDS             3369..4139
                     /locus_tag="PKI50_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNT02435.1"
                     /translation="MQDTSELRFAVLIDAENIPYKNIRGILAEVAKYGTPTLKRIYGD
                     WTNSSLSGWKSNLLENAITPIQQYSYTTGKNSSDSALIIDAMDILYSEKVDGFCIVSS
                     DSDFTRLATRLREAGMKVIGIGERKTPSAFIAACDKFIYIEIINAPVKNSDQDSNELH
                     RDDTVEQDHKNSLDIGNDIIRLISDSVNDVADDNGWAFLADVGNLISKKQPDFDARNY
                     GFFKLTPLVKSLKCFQIEERESGNSRIKHIYIRNKNKQ"
     gene            4175..4906
                     /locus_tag="PKI50_01555"
     CDS             4175..4906
                     /locus_tag="PKI50_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02436.1"
                     /translation="MRTYKRMYIKLMLSVTAVLGICFIALDFSRFLNQNYSYDIFPGA
                     ILKRICVIIAAVIAWSVGRDEINPKDSRLMKAAFIFVILGEAAFFIRERAIGIGMFAL
                     CQTLLTVRNGTGLRYKLARAGSNQKIGLIVSGIIVIISVIFPFLTGYLFKTDITLNIA
                     YLYGIILSISLWAGLACNILGLLPQRNAGMAALGMTCFFCCDVLVGLDAVLEAGLPWL
                     LANSFIWIFYIPALVLLALSCYRYN"
     gene            4923..5354
                     /locus_tag="PKI50_01560"
     CDS             4923..5354
                     /locus_tag="PKI50_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT02437.1"
                     /translation="MKGTIYAIAAGIFIAMQQVFNARLGEKAGLWETNTFVHGSGFVF
                     SLIILMIAGNGSFAKIGETNRLYLLGGVLGVLIVFSAMIAVSALGATYSIAILLVTQL
                     IAATVIDSFGLFGSPVIEFDITKLLGIAIMIAGIIVFKLKG"
     gene            5676..6344
                     /locus_tag="PKI50_01565"
     CDS             5676..6344
                     /locus_tag="PKI50_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT02438.1"
                     /translation="MLLIIAALLLFIFALRTISINTLHYYDPIYKGMEDDKKIAFACN
                     VVWGEEYLPQMLKLLEEKDIEITFFIGGQWAEKNEELLRNMHKAGHELGNHGYKHLYH
                     SKLTPESNKQEISKTEEIIKRITGYKTVLFAPPYGDINGTVVSSAEALGYKVIMWSID
                     TIDWNTRDYKKILQRVENKHHKGAIVLMHPTKVTVAALPQLIENLASHGYEITTVSDV
                     LEND"
     gene            6414..7670
                     /locus_tag="PKI50_01570"
     CDS             6414..7670
                     /locus_tag="PKI50_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT02439.1"
                     /translation="MYLKETLKNGIRIVAEEIPYVNSVSIGLWFKVGSRYESIENNGI
                     SHFIEHMLFKGTKNRSAKEIANSIDKLGGQLNAFTSKESTCLYAKVLDTHFDIALDVL
                     ADMFFNSNFDCDEIDKERGVVIEEINMYEDSPEDLVHDLFSQSVWSGNPLGMSILGTE
                     ESLENLNRDIIVRYFKENYSPKNIVISVVGNFKHKDAFDKIKAYFEKAELINRSATDI
                     AVPRFEPEYTLKSKDTEQVHLCMGFNGVDIKNKAFYPMLVLNNVFGGAMSSRLFQKIR
                     EDRGLAYSVFSYPSSFEDCGLFSIYAGSKPDNLKSVAKLIMEEINMIKDKGITEEELY
                     DSKEQLKGSYILGLESTSGRMISIGKSELLLDVIYSPAEILAKIDKVDMDNVNSTIKH
                     IFDTDSMGAAVIGNMKNGTDIRNLFK"
     gene            7690..8148
                     /gene="dut"
                     /locus_tag="PKI50_01575"
     CDS             7690..8148
                     /gene="dut"
                     /locus_tag="PKI50_01575"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNT02440.1"
                     /translation="MKDVTVKYIRTSPDENPEPKIPAYTTEGSAGMDLSACISEDIII
                     KPGEIAMVKTGIAIQIPDSSIGGFVFPRSGLSSKHGISLANCVGVIDSDYTGEIICPV
                     INHGSSDYTIKNGDRIAQIVFMPVVNVRLQKADKLEATGRGAGGFGSTGK"
     gene            8216..8464
                     /locus_tag="PKI50_01580"
     CDS             8216..8464
                     /locus_tag="PKI50_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HNT02441.1"
                     /translation="MRFSKLGGKEIVSLPDCERLGYLGDCDLMIDGETGRIKSLIVPE
                     NKGTLSFFIDRRYIEIPWERIKKIGNDMIIIDFADILK"
     gene            8670..9548
                     /gene="dpsA"
                     /locus_tag="PKI50_01585"
     CDS             8670..9548
                     /gene="dpsA"
                     /locus_tag="PKI50_01585"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HNT02442.1"
                     /translation="MKSSRYLIIGGDDRLVELAKLFEGEGADIATYGMDMTVIKNVPN
                     YLSFDEALENSDIVIGPIPFAKDVHKINSKYSSVDIEAEELFSKLGKDKKLILGAINN
                     YSKGLAEKYGIEYWDYHNDEGYQILNAIPTAEGALSILVNETKTTLFGSRILILGYGR
                     IGKLLSEYLKALGASLYVEARKDSDLSWITAERMKAIPLKELPQYLGEMEIIINTVPA
                     MLLDSNMLDLVKQEALILDLASAPGGTDFDCASKKGIKAIHAQGIPGKAACKSAAEYI
                     YSTIKKYLCADRQKGV"
     gene            9551..9865
                     /locus_tag="PKI50_01590"
     CDS             9551..9865
                     /locus_tag="PKI50_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNT02443.1"
                     /translation="MKVEGLNIGLAVTGSFCTFDKIIPEAEKLIKQKANVYPIFSTNA
                     ASMDTRFGKAEDWLRRFEEITGNSVIKTIEDAEPIGPGNMLDVLVIAPCTGNGFTISK
                     LR"
     gene            complement(9843..10952)
                     /locus_tag="PKI50_01595"
     CDS             complement(9843..10952)
                     /locus_tag="PKI50_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNT02444.1"
                     /translation="MADKESLRAALYIRVSTEEQALDGQSADAQAETLKQYCIAFGIS
                     IYDYYMDLGVSGKSMKDRHELKRLIEDCKSRFFDIVLVWKISRLSRNLKDLLYLIDIF
                     ENNGVCFASCSEKFDTSTPIGRMTLQLLGSVAEFERNTIVENVKLGLKEFARKGGKSS
                     TVLGYDNIDKKLVVNEEEAGIVRLIFRLYAEGGMNCSAIAGYLNSMGLKSKRGCEFRG
                     SSISYILHNTVYIGINRHLIGKENQYSVKGTHDAIIDDELWNKAQKASTGTRKRTSRK
                     ASDYSGMQLKAFCMKCGMPLTSFHASSKGKKYIYLRCRNCSNYVNAEKLTKAVLEAIE
                     PITGKKEPSMHRQLPELPIKRIEAYKGDVNVVLKL"
     gene            11135..12460
                     /locus_tag="PKI50_01600"
     CDS             11135..12460
                     /locus_tag="PKI50_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HNT02445.1"
                     /translation="MSIIDTFLLRYAKEYDFYNELAHQVAMICETIIHRSGIRAIVTY
                     RAKKPESLKDKLMKRNAIKKYQSIEQIYRDIVDLSGVRIAIYFPGDREEIGRLIENEF
                     ITRKTKKFPNSEQKQHQDNPFKRRFTGYDAVHYRVKIKEEKLEENNKRFAHAQVEIQI
                     ASALMHAWAEVEHDLAYKPQIGKLSEEEFNILDELNGLVLSGDITLERLQKSFKQRIA
                     SMEQQFNNHYELAALLREKISGSFPEGCFIGRVDILLKFLQKIGMDNPKEIHMYLDSF
                     DKMKESVGSKTIVDCFADLILNHYPDYYRQYVEAKFESSFNNPYISIQKQMLLGKENE
                     QLNSFVEKWILFENAIKALEQKNITQSRSISDIIRRHLTADEIILDTVKNLEKTRNHI
                     IYSSDIPDNLQLRNAADSITLILNKLAELASDENKTVICKALDKIIKEF"
     gene            12478..12789
                     /locus_tag="PKI50_01605"
                     /pseudo
     CDS             12478..12789
                     /locus_tag="PKI50_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
     gene            12804..14045
                     /gene="dapG"
                     /locus_tag="PKI50_01610"
     CDS             12804..14045
                     /gene="dapG"
                     /locus_tag="PKI50_01610"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNT02446.1"
                     /translation="MKIIVQKFGGTSVSTQERRELVVDKIESAVKKGFLPIVVVSAIG
                     RKGDPYATDTLINLAASAGVKNNPRELDMIMYCGEIISCVVLSNTIKKRGYDNIVLTG
                     GQSGIITDEKFGDAEVLRVEPHYIKECIEKGLIPVIAGFQGISEKGEITTLGRGGSDV
                     TGAIIGEALNAEAVEIYTDVEGVMTADPKIVPDAAVIGTIYYNEVFQMAEYGARVIHP
                     RAVEVAMRSNLPIFIKNTMSDSSGTLITNCDKNRRYRYDKNDKLLTAVAHVGGRTRVR
                     ILFDGSAKEYENDSHLFSTIASEGISIDMINVNPDFKLFIIVDDDVEKLGTVLNRCGY
                     NYELKKDCSKVTIIGNKMRGVPGVMAKAIRALAKHNIEILQTSDSHTTISCLIESINT
                     NTAVNALHHEFELGKPAIQNL"
     gene            14246..14953
                     /locus_tag="PKI50_01615"
     CDS             14246..14953
                     /locus_tag="PKI50_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02447.1"
                     /translation="MDKIIQLKNIRKVYRMGNEKVIALDNISFEIEEKEICCLLGTSG
                     SGKSTLLNLMAGLEKPTKGEVKIKNYNLESLNEKQLAKLRQKYIGFVFQSYNLLEHFT
                     ALENVTLPLIFKGVPKSVREKKAKALLKAVGLGDRMNHKPSQMSGGQQQRVSIARAFI
                     SNPEIVYADEPTGNLDTRTSTETMDLITGMAEEYKQTLIIVTHDLEIAGYANRIIYIK
                     DGNVEKIDMLSEKAGRC"
     gene            14955..17339
                     /locus_tag="PKI50_01620"
     CDS             14955..17339
                     /locus_tag="PKI50_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02448.1"
                     /translation="MKRKLFIVFLAAIIAFAGLPAINVSAGIYDDIEVSYDVNESRIL
                     DGDSFTLYFTIDEVRAGEISDVEINIETGSNFKVLGGNLNIPAAVLDKQYNVNLQYLG
                     GSNSLPIKIKYDKNSVYEGEFIYNVEIAEIDEESVNPSSSSSDNEPKLIVESSSIPVA
                     AAGETMKIPITVTNESRYSAKDVTVKLSLANNDNNPFEISSLNLVQSIGSLNAKKSDG
                     LIFRLKVNPYAEEKTYELDVQFSYYNSKNNLFTSNEKIYVKVTNSKNEPVLMIQDIKH
                     STYPVNAGAKFQISFNAANVGGIDARGVKVSIEGLNKDGFTLYSGVNSWYFDNIEGNG
                     KKNITVDLVAADSMESGNYGLEIKMEYKDQRNNSYTASSSFFVTVKGEAKSSNVTIKN
                     ISVLPQEVRANENFTLSLDVLNEGKSSARNVKLTIKGDEGIISKSPDVRIIENLPAGE
                     QQKLEYVLYANNETKTQNYNIMINLEYEDADKENSKHSVNQYAGVYVNGGNSKITPKI
                     IISNYNFEPKLVRAGEEFSMDLTFMNTSSTKEVKNIKIYLTGIDSDKEGNVVFTPVGS
                     SNTFFVDAIKPKGTAVRSITMYTIPDAQPKYYNITANIEYQDDEGTEYKAEELIGIPV
                     IQQSKLDASEIGFPPETFIGQPIPVSLQYYNKGKTRLSNLMIKIEGDFRIENGEAFIG
                     NFESGSSDYYEAMVTPMKPGPAKGKVVFSYEDPSGDEFTYEREFSINAIEMPPMEMPG
                     GEMPMPPVSTKDKIMKYTIKNWFFWVCIAVIAAGIFTARRLIRNKRRKDMDLDE"
     gene            17332..18696
                     /locus_tag="PKI50_01625"
     CDS             17332..18696
                     /locus_tag="PKI50_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02449.1"
                     /translation="MNRIDIVRIVLKNLLRRKMRTMLTVMGVVIGTASIVIMVSIGIG
                     MNEGFKSEISRMGSLNSINVRSPETGMYYIYDETEAGRTNSTEMRDLDDAAVADFSRI
                     AGVESVIPFIETHGKIKLGKYIGYVQLQGINPEHMDKLGLKVSEGRLLGAGDKMNTVF
                     GFGVKDNLYNPKGSQRRRFGRNTPEVDLLGERITLSLDMSYGEEFIQRDTSVQKKPKT
                     YKINGVGLLEEGSYEYYSSIFMDITELKKIVKDFNKGKSKNDNSGDYRDIKGYSRIIV
                     KVKDINNVQRIKNQIKDLGFFADSLTDILESMQKTTSSIRIVLGAIGAVSLLVASIGI
                     TNTMVMSIYERTREIGIMKVIGSSLGDIKRMFLFEASMIGLLGGLIGLLLSYGVSYGL
                     NTAGFALIGFFGPSGGGSKPSIIPLWLAVSAMLFTTIIGLVSGFYPAHRAMNLSALEA
                     IRKE"
     gene            18819..19535
                     /locus_tag="PKI50_01630"
     CDS             18819..19535
                     /locus_tag="PKI50_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNT02450.1"
                     /translation="MTEADGADKEKEAKNNANENIKELGEVRVPQFRSNIHCITIIGQ
                     IEGHMVLPPQNKSTKYEHIIPQIVAVEENPEIEGLLLILNTVGGDVEAGLALSELIVS
                     MKKPSVSLVLGGGHSIGVPLAVSATHSYIASTATMTIHPIRMNGLVIGVPQTFDYFNK
                     MQERIIRFIVEHSNIDVDTIKRLMFTTSELANDIGTVLVGAEAVKYGLIKEIGGLHQA
                     LAKLQNLIAASKAAENQRSN"
     gene            19696..21930
                     /locus_tag="PKI50_01635"
     CDS             19696..21930
                     /locus_tag="PKI50_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNT02451.1"
                     /translation="MSGKRKENDLNYEISGVSLILASIYIYISLFSSNSGIVGEKVRL
                     FFLGTFGLGAYIVPVLIIILGLALVYRKNEIRINIKFFSFCIFLLSTLIVLFLSNYTE
                     IENADAKWLDAVRYYFSLGILNAGGGAVGALFGYAFVRLFGMIGTIAIVSSLYIISIL
                     MFTEVSSMRILQYIKQSASDIYSRLRNKNKEAAVTKELQRKIPVKHHNDIESSEADDE
                     ERIKNIDKKIKILDFTKRFDKDFKGTAENEADDKARNKDKTSRENIPSIVPVSNIHYV
                     NYKLPGTGLLALPAENKSGNGKKEVMNNVKILEDTLKNFGVSASIAQVSVGPTITRYE
                     LQLSPGVKVSKILSLADDISLSLASPDIRIEAPIPGKAAIGIEVPNKDITSVLLREVL
                     ESEEFRKHKSKISFALGKDVAGANIVADLAKMPHLLIAGSTGSGKSVCINTLIASILY
                     KATPEEVKLLLIDPKVVELSVYKNIPHLLVPVVTDPKKAAGALNWAVTEMTERYKKFA
                     ANNVRDIYGYNNLNKEELAKMPQIVVVIDELADLMMVAPSEVEDSICRLAQMARAAGI
                     HLVVATQRPSVDVITGLIKANISSRISFAVSSHIDSRTILDMGGAEKLLGKGDALFYP
                     VGAAKPVRVQCAFVSEKEIEKLVAYIKEQVPASFDNKIIKEIEQSVEIDEDAEADELL
                     NDAIRIVVENGQASILMLQRRLRIGYSRAARLIDQMEERRIIGGYEGSKPRKVLLGRE
                     DLED"
     gene            21952..23289
                     /gene="rimO"
                     /locus_tag="PKI50_01640"
     CDS             21952..23289
                     /gene="rimO"
                     /locus_tag="PKI50_01640"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNT02452.1"
                     /translation="MSLKVAIISLGCSKNLIDSEVMVKSILNNDYEVTDNANIAEVII
                     INTCGFIESAKQESIDTILEMSDYKKNGRCKVLIATGCLAERYRDELIKELPELDAVI
                     GTGDYKDVPEVISKALKGEKVVLYGHQELIDIDKLPRHISGFGASSYLKIAEGCDNRC
                     SYCIIPALRGRYRSRKTEDILEEAKELSRNGIKEVNVIAQDITRYGLDLYKSRQLSEL
                     LKKLAEINGIEWIRLLYSYPDEFNDELIEVIAREDKICKYLDIPIQHTSNRILKKMGR
                     RSSREQIQRLIEKLRKRIPGIVLRTTLIVGFPGETEEDFKELYDFVSETKFNRLGVFA
                     YSREEDTPAFNMPNQVEEAVKQERLEKIMLLQKDISLEYNRKLVGQTLKVLVEGASDN
                     EYSGRSCMDAPEIDGKVYFKSNKEIIPGDFCSVTIKKAYEYDLMGERTDEPGK"
     gene            23273..23815
                     /gene="pgsA"
                     /locus_tag="PKI50_01645"
     CDS             23273..23815
                     /gene="pgsA"
                     /locus_tag="PKI50_01645"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNT02453.1"
                     /translation="MNLANKITIIRILMIPVFLLFAVAEFPYHMTAAVAVFLAASLTD
                     KLDGYIARKYNMITDFGKFMDPLADKLLVTAAFLVLIQLGRIEAWIVFIILAREFAVT
                     GLRTLAAAQNIIIAASSYGKIKTVTQIIAIVILLMDNYPFSLLNLPVDAVMTYAALLS
                     TVISGIDYFKKNMQVITRMK"
     gene            23838..25073
                     /locus_tag="PKI50_01650"
     CDS             23838..25073
                     /locus_tag="PKI50_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNT02454.1"
                     /translation="MKSEIIAVGTELLLGNIVNTNAQYLSQKLADLGIDVYYHVVVGD
                     NLKRLTETIKTSLERSDIVITSGGLGPTADDITKEGAAQAMGLKLLPDEESIERIKKM
                     FSSTGRIMTENNIKQGYIPEGAVVLENNNGTAPGVLIEKEGKIVIMLPGPPKELYPMF
                     ESKVLPYLKSKTDSTIRSKVLRVIGVGESAVEHILKDIFDSQANPTIAPYAKDGEVHL
                     RITAKTGIPEEADSLIAEMEQKVRAILGDNIYGCNEETLEEAVLKLLQKKNLTISLAE
                     SCTGGLVASRLTDIPGASASLISGVISYSNESKINMLKVKEETIRKYGAVSPQTAEEM
                     AVGAKRLSNTDIGLSITGIAGPDGGSVEKPVGLCYIGIAIGKSVNVQKIMLTGNRNRI
                     RWGSSSRALDFLRRELLSG"
     gene            25207..26244
                     /gene="recA"
                     /locus_tag="PKI50_01655"
     CDS             25207..26244
                     /gene="recA"
                     /locus_tag="PKI50_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNT02455.1"
                     /translation="MNDKKKAIELALSQVEKQFGKGSIMKLGDATKMNVEAVSTGSIE
                     LDIALGIGGVPRGRIVEIFGPESSGKTTVALHVIAEAQKNGGEAAFIDAEHALDPLYA
                     KNLGVDIENLIVSQPDTGEQGLEIAETLVRSGAIDVVVVDSVAALVPKAEIDGEMGDA
                     HVGLQARLMSQALRKLAGAISKSRTTVIFINQLREKVGVMFGNPETTPGGRALKFYAS
                     VRLEVRKIENIKLGNDLTGSRTRVKVVKNKVAPPFKQAEFDIMYGQGISKEGSILDAA
                     VSNDLILKSGAWFSYGDIRIGQGRENAKQYLKDNPDITKELDKKLREMFNLGISKAKA
                     ADFDSEENDEF"
     gene            26260..26883
                     /locus_tag="PKI50_01660"
     CDS             26260..26883
                     /locus_tag="PKI50_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNT02456.1"
                     /translation="MRITGIEQQKKNTKRYNIYIDGEYKCTIEDNILQELNLSKGMEL
                     NEIEFNNTLEIIQYKGALRAALFMLARAPKTEMEIEKGLSDKQHAPKAIKKVLEYLKE
                     IGYINDESYAESYIRSIKATTGTSRRSIYQKLAAKGIDKEIIQQMLNKAEIDDYASAI
                     SAAKKKAASLKGNRREKKLKLLNYLYRKGFGIDDCKRAAEDILQEED"
     gene            27120..28649
                     /gene="rny"
                     /locus_tag="PKI50_01665"
     CDS             27120..28649
                     /gene="rny"
                     /locus_tag="PKI50_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNT02457.1"
                     /translation="MEYVIYIAIALITFIFGYLVRRYIAESKIKNAEEQALKIVSEAE
                     KQSETKKKEVVLEAKEEIHRLRAELDREIRDRRNEIQRAERRLLQREESLDKKVENLE
                     QRDEALAKKQKEIQKSLEEVQQLYKKQVEELERLSGLSSEDAKQLLLNNIEKQIRHEA
                     AMMIKDIETKAKEEAERKAKEVIASAIQKCSADHVAETTVSVVPLPNDEMKGRIIGRE
                     GRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREVARVALEKLIVDGRIHPARIEEM
                     VDKAKKEVDNYIKEQGEQATFDTGVHGLHPEIIKLLGRLRFRTSYGQNVLKHSIEVAH
                     LAASMAAELGADVKTAKRAGLLHDIGKAVDHEVEGPHVQIGADLAKKYKESADIVHAI
                     SAHHGDIEATTVEAVLVQAADAISAARPGARRETLESYIKRLEKIEEIANSFDGVEKS
                     FAIQAGREVRIMVKPEDVSDSEIVYIARDIVKKIEDELEYPGQIKVNVIRETRAIEYA
                     K"
     gene            28790..29569
                     /locus_tag="PKI50_01670"
     CDS             28790..29569
                     /locus_tag="PKI50_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNT02458.1"
                     /translation="MNILFIGDVVGRPGRNYLSTNLSRLVEENNTDFVIINGENSAGG
                     VGITRSTYDELLSMGADIITLGNHTWAKKEVLEFIEDAERLIRPANYPEDIPGKGYRI
                     LEKAGKRIAVINLCGRVYMDCIDCPFKAIDKILNELKNKADIIIVDFHGEATSEKLAM
                     GWYLDGRVQAVLGTHTHVQTSDERILPGGTAYITDVGMTGPIDSILGVDKEAVIRKFI
                     TGMPAKFEITGGEVAVGAVCIQLDDNNKVTSIKRFTQNSKL"
     gene            29660..29920
                     /locus_tag="PKI50_01675"
     CDS             29660..29920
                     /locus_tag="PKI50_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNT02459.1"
                     /translation="MDVLKVSAKSNPNSVAGALSGVLREKGSAEIQTIGAGALNQAIK
                     AIAIARGFVAPGGIDLICIPAFTDIEIDGEERTALKLIIEPR"
     gene            30102..30926
                     /locus_tag="PKI50_01680"
     CDS             30102..30926
                     /locus_tag="PKI50_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT02460.1"
                     /translation="MQKVDLHIHTTASDGLMSPEKVVRWASSKRLTAIGITDHDTVKG
                     IGPAVKAASRYGVEIVPGIELSTLFEEEEIHVLGYFIDYKSQWLADTLKKIQNSRYER
                     AGKIVGKLNDMGIDITLEEVKGIADDGAMGRPHIARAMIDKGYISNIREAFAEYIGKD
                     RPAYVERYKLTCKEAIDMIEKLGGISVLAHPGLIKNKDNIGKIIDLGIDGIEVYHSKH
                     DDETVKNILAVAHSRNLLITGGSDCHGMKFNNEPILGNYSIDYKYVQMLKEALNKI"
     gene            30940..32142
                     /locus_tag="PKI50_01685"
     CDS             30940..32142
                     /locus_tag="PKI50_01685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT02461.1"
                     /translation="MEIKLSDKVLNIAPSMTLAVNAKLKQMVDEGVEVFGFGVGEPDF
                     DTPEYIRNAAVEAMNKGFTRYTPAQGTIDLRKAICAKLKRENSLDYEPNQIIVSSGAK
                     HSLSNAFAAILNPGDEVIVPVPYWVSYTEIIKLNDGVPVLVQTKKENNFKMSGEELKN
                     AITAKTKAILINSPSNPTGAVYTEVELREIAEIAVQNNLFVVSDEIYERIIYDDNKHI
                     SIASFNSEIKDLTILINGMSKSYAMTGWRLGYAAGNEKLIKAMSSVQGHAVSHPSSIT
                     QYAAAAALNGPQDDLKNMVQEFNKRRNYMYGRINAMKGLRCIKPEGAFYVYVDISSYL
                     GRNLCGIKLDSCLDFAQVMLEKGHVAVVPGAAFGTEGFVRISYATSMEIIKNGLDAFE
                     SFIYKNIE"
     gene            32157..33590
                     /gene="purB"
                     /locus_tag="PKI50_01690"
     CDS             32157..33590
                     /gene="purB"
                     /locus_tag="PKI50_01690"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNT02462.1"
                     /translation="MDRKEYQNPLISRYASKEMSFIFSEDYKFSTWRKLWVALAESEM
                     ELGLNISPEQVEELREHIYDINYEDSEKREKETRHDVMSHVYAYGLQCPKAKPIIHLG
                     ATSAFVGDNTDVIQMSEGLRLIRTKLLNVIKNLAGFAEKYKNHPTLGFTHFQPAQLTT
                     VGKRSCLWMLDFLMDMEELDFQLGKLKLRGVKGTTGTQASFMALFNGDHEKIKELDAR
                     VTKKMGFEKSFEVTGQIYPRKLDSQVLNVLSQIAESAHKFSNDIRLLQSLKEIEEPFE
                     KGQIGSSAMAYKRNPMRCERIAALSRFVITNSLNPAITAAAQWFERTLDDSANKRLAI
                     PQGFLAVDSILNIMINVTAGLVVNEKMIKRHIDEELPFMATENILMEAVKAGGDRQEL
                     HEKIRQFSMEAGKRVKEEGLDNNLISLIEGDESFRLGKNKIQELLDAGNFIGRAPEQV
                     EEFLAEHVYPLLEKNKDKLNITADLKV"
     gene            33619..35010
                     /gene="asnS"
                     /locus_tag="PKI50_01695"
     CDS             33619..35010
                     /gene="asnS"
                     /locus_tag="PKI50_01695"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNT02463.1"
                     /translation="MSSIPIKQLYRSTEDYISKNIKVSGWVKTERTSKNFGFIELNDG
                     SFFKNLQIVFEEDLENFNDIAKLSVGSAIIAEGLLTESLGAKQPFELKAVRITIEGKS
                     TPDYPLQKKNHSFEFLRTIAHLRPRTNTFSAVFRVRSLVAYAIHRFFQEQGFVYVHAP
                     IITGSDAEGAGEMFRVTTLDIDNPPRTEKGEVDFSRDFFGKSANLTVSGQLNAETFCM
                     AFRNVYTFGPTFRAENSNTARHAAEFWMIEPEIAFAELSDNMELAENMVKYIINYVLE
                     NAPEEMEFFNRFIDNTLLDRLKNIVNSDFGIVTYTEAIDILKKSGVDFEYPVEWGIDL
                     QTEHERYITEQVFKKPVFVINYPKEIKAFYMRMNDDGKTVAAMDLLVPGVGEIIGGSQ
                     REERLEVLEKKMDEMGLNKEDYWWYLDIRKYGGVKHAGYGLGFERIIMYITGMGNIRD
                     VVPFPRTVKSAEF"
     gene            complement(35110..35352)
                     /locus_tag="PKI50_01700"
     CDS             complement(35110..35352)
                     /locus_tag="PKI50_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02464.1"
                     /translation="MSFKTNPLTVLLIPPNNSPVGSADREKGRGNRLRAGEAAGYDDG
                     GHAAGGNALYADECLRPIIKPPFKGIWNEMNPYSII"
     gene            35524..35599
                     /locus_tag="PKI50_01705"
     tRNA            35524..35599
                     /locus_tag="PKI50_01705"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35557..35559,aa:Trp,seq:cca)
     gene            complement(35848..36636)
                     /locus_tag="PKI50_01710"
     CDS             complement(35848..36636)
                     /locus_tag="PKI50_01710"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNT02465.1"
                     /translation="MRKGLIAAVLLLFPAFSIDIYAEDTELIKELMACPEEETFSGLN
                     SDNYIEALKSMGYYKYDYKDELINLRNATLRFQSDCNLAADGIQGPQFKAAMIKRLSL
                     NGNYRPPDIIARPASRGYWIVINRTKRILTLYSGKTVIGKYPIAIGKATNKTPQGKFH
                     VCLKRKNPVWSPGDGSKPVAGGSPKNPLGTRWIGLSVGKGNLYGIHGNNSPYSIGRQV
                     SKGCIRMINSDVLSLYNIIPVGISVWIGNDETLRKWGVTQKSFY"
     gene            complement(36900..37115)
                     /locus_tag="PKI50_01715"
     CDS             complement(36900..37115)
                     /locus_tag="PKI50_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HNT02466.1"
                     /translation="MARNNQKVVPHAAAALDRFKYEIATEVGVTLKEGYNGDIAAKDA
                     GKIGGQMVRRLIETAERNMAGTTGTRF"
     gene            37339..37989
                     /locus_tag="PKI50_01720"
     CDS             37339..37989
                     /locus_tag="PKI50_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02467.1"
                     /translation="MKGVKPSVKALCSALAVCIIFSAGIAAFADNNAPSAVKSKHYLE
                     EAVEKMVQEGSLTREKADKILEYKQKRNEKLKKLPIEQKTQMKKQGGRGSLLKALVEE
                     GIITEEEAKSIREKLREMKEARVADGLQGLIDKGVLTKDDINNIRSYLLKVREERKSS
                     VEKLKSMTPEERKAYFKEHKNERKDIITKMVEDKVITKEQAEEIRKAIPELSKFRY"
     gene            complement(38118..39566)
                     /locus_tag="PKI50_01725"
     CDS             complement(38118..39566)
                     /locus_tag="PKI50_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT02468.1"
                     /translation="MKSNTIKWRIFKYNLTVIITLIVLVAIIFNAAIRIYIEKDIIGQ
                     LNKIASHAEETALKRGPEIFIFSKEIPPELPRIRIRTNDVFRFYFMLDRSLREPLSVL
                     NADYILMDNNKNIINSPVELYADPDEGLVGKISSEISKLKDLEKESYLNFHLSGTEYI
                     AVVKPVAQKNDFGLGWIIIYSSLQKVNQLQMAINIILFAILLLSAFIIVLFSSVIVKK
                     ISEPFSSLNQHIRSIAERNFGAKISTPVYDELSEFVTNINIMSERLETYDKAQKTFLQ
                     NVSHEFRTPLMSIQSYAEGIKYNVADSNEAAGIIIEESKRLTQLVENLLYLSRLDAME
                     ENYCLTTLDYNELLRSCAERMNMIACNNNIKIDISVPEENINIIADEEKLSRAINNIL
                     SNCIRYAKSSIEIKSEIIDEKTVRVTISDDGPGFESNELPNIFDRFFKGRKGNFGLGL
                     AISKCIIEKHKGKITAENSEAGAVFRIDLPMA"
     gene            complement(39571..40266)
                     /locus_tag="PKI50_01730"
     CDS             complement(39571..40266)
                     /locus_tag="PKI50_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT02469.1"
                     /translation="MDKKLVYIADDEVNICNIIKSFLIKEGFEAETFNDGQSILDAFN
                     VKPADMLVIDIMMPGIDGYSLCRQVRQISSVPIIIVSAKDTEPDKITGLTLGCDDYLT
                     KPFSPMELIARIRSIFRRIELDKAQTGIGEVIEVSDIVIDHNIKKAKCHDKDIGFTGM
                     EFSLLYYLAANRNRAVSRSELLDKVWGFESEVETRATDDMVKRIRKKLADAGSRLKID
                     TVWGFGFRLTGKE"
     gene            40433..42160
                     /locus_tag="PKI50_01735"
     CDS             40433..42160
                     /locus_tag="PKI50_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNT02470.1"
                     /translation="MNSNTVPVRKHLIKLSRKKVLIAAAILLVAASVIGFNYYKSKNS
                     KNPAVQQATAIARMGDLSVVITGSGPISSSEKFTLTSNVNGTLTKIYFQDGDKVNAGD
                     LIFEIDDKDTQLQIKQIKNSIAQAELTRSSNISDLEAGTVTAPIDGEIHDMQVKEGDN
                     ISNNGTLFVITDKSELKLLVPFINTYRNRLSIGQKVAINAFDTERDELHRMEGTISGI
                     STPGYTTSSGAESYNVSIVIENNGSLKEGMIANASVDIDGTEVKSSGSNTLSYLKNMT
                     VRAPSGGTVSKLYAENGQNVKKGDILAELENKDLELAIKTNDLKLEDLNIQLQTAEEK
                     LLDHKIYAPFSGTLTLNDIEQGNSIKQGDILGSIANYDIMEFSINVDELDIAKIKEGQ
                     NVRVTIDALPDTTDKPLKGIVSKIAVEGTSSNGVSIYPVTIKIEKNEALKGSMSANGE
                     IIVNEKKDVLYVPIDAVVKMNGKDYVRVVPGAGGAGKAARNRNSDANGTEVKEDTKQN
                     TELQVELREVKTGISNAEYIEIVSGLKEREAVIVTSRSSGSNNQRGPNMMIMGTPPAG
                     GGNVRIRNN"
     gene            42174..42851
                     /locus_tag="PKI50_01740"
     CDS             42174..42851
                     /locus_tag="PKI50_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02471.1"
                     /translation="MIEICNMSKIYRIGNVAVKALDNISLSIKQHEFVSIVGPSGSGK
                     STLMNMIGCLDVPTSGEYCLDGMEVSGLTDDQLAYIRNHKIGFVFQGFNLIQKLNALE
                     NVELPLIYQGIPTMERYERSVEALKLVGLEDRIHHRPAELSGGQQQRVAIARALVGNP
                     QIILADEPTGNLDSKSGKDIINLLKELHLKGSTIVLITHDNDIAMMAERIVRIQDGQI
                     NDDRMVS"
     gene            42854..44032
                     /locus_tag="PKI50_01745"
     CDS             42854..44032
                     /locus_tag="PKI50_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02472.1"
                     /translation="MSFIQACKMAVKSIKSNKVRSFLTMLGVIIGVSSVIAAVGFAQG
                     STKSITDMISSMGTNLIQVNIMGRNSNRNVTYSQIKEFAEENSGEITAVAPQSNTSAT
                     VKYKTNSRSTNVLGTSPDYQIIRSVYAQQGRFLIQNDLENMQKVAVLGTAVVNDIFEG
                     TSPLGEKIKINGQLFTVVGILEEKDGGQDQGADDIVIIPATVAQRLFRSGIIRNFSIQ
                     ATSPDTVESAMEKLEEFLAKTYKDSDAFRVFNQAEMLENLDSMTGTMTAVLAGIAAIS
                     LIVGGIGIMNIMLVSVTERTKEIGIRKAIGARKKNILVQFLIEAIILTGFGGVLGVAV
                     GLSVIHFIIGGFGLVPEVYSIPWILISFGISLGVGVTFGLFPAYKAASLNPIEALRSE
                     "
     gene            44046..45197
                     /locus_tag="PKI50_01750"
     CDS             44046..45197
                     /locus_tag="PKI50_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT02473.1"
                     /translation="MRRTFFILSAILCVSLLFNCIGAFADATEDGLLTLEEAKAMALK
                     NDVHYHHQDENIQAALEDYEKLSEDTSINSGRGSNIAQKEAARISKKIQLENAYSSVR
                     KVVLTKADLKRASYSDTANAFYAVIEAQNSLASALTDLELKRKELEKAEIKYMLNIVP
                     KKTLTQAEEACKNSEAAYNKAALELENCISKLCKIIGRKLDMSKVKLDTTLDIPDIKS
                     IDLDKIKEDNLKNNLSYFSAREQYKLAEYQLALTEEKYEDYYDDLKKGSSNVREEFDN
                     MLYDAQKKFEDAEYSWNEKLEDLEESLREQYESLEDLYENYLEQKEDIDDMKLTVKEN
                     KTKHAMSIITKAALDSSIASLERLERQLVSTIIKLNKQYISLVQCSIEE"
     regulatory      45367..45446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            45523..46467
                     /locus_tag="PKI50_01755"
     CDS             45523..46467
                     /locus_tag="PKI50_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT02474.1"
                     /translation="MNNLVSKITDLFIIKFIKNTKPEKKISDPATRRKYAYLEAYVSI
                     VGNLLLSAVKVALGLFLNSISLLADAAHTAADVLTSIVVLFGFKLSSSPADEKHPFGH
                     GRIELIATLVISAMLFLVGFEFAKSSYERLLSNTPVKGSYIIALIMIFGAIFKEWMAR
                     FSIDLGSRTSSSTLIADAWHHRTDGIASILVAAAIIASKFGYYKVDAVFGLMVSALII
                     YTGYSISKDSISQIIGETPAEEELNNIENIALTVSGVHSIHKVNVHSYGCHKEISLHV
                     QVDNDMSLVMAHDISEQVERIIEENMNCKAIVHIEPLE"
     gene            47309..48259
                     /locus_tag="PKI50_01760"
     CDS             47309..48259
                     /locus_tag="PKI50_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNT02475.1"
                     /translation="MTKLPDIAVEFLEYMETIRGKSKNTITAYSYDLALFFKYIKMKR
                     GYLVTDMEIDEIPIDDVDMYMINNISLADLYSFLSFAANQRDNKNYARARKVACIRSF
                     FKYLHKMKMIKENPSVELESPKINSRHPIYLTLEESKKLLSSIDGENKERDYAIITLF
                     LNCGLRLSELISIDINKISGDILTVIGKGSKERTVYLNKACIDAINNYLKVRPVDDVK
                     DKNALFLSERKRRISKRTVQYLVKKYIGISNLDMEKYSPHKLRHTAATLMYKYGKVDI
                     RSLQQILGHENISTTQIYTHLDDETLRKAINANPLSEEKK"
     gene            complement(48293..48550)
                     /locus_tag="PKI50_01765"
     CDS             complement(48293..48550)
                     /locus_tag="PKI50_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02476.1"
                     /translation="MEYKKECVLTDRECTDCGECDRCDIDPNKICDNCCTCIDKAADY
                     NSIEIDEIIEEEDAELDMEDHGKWKYERDYIVDYSEKEGND"
     gene            48725..49915
                     /locus_tag="PKI50_01770"
     CDS             48725..49915
                     /locus_tag="PKI50_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02477.1"
                     /translation="MINASANIFVFTAYVSLLIIEFVFLKILYIDFNTPWLITTLIYV
                     LFIIYQILVRVNEQVEKGRVKEILYCIIDSINGIPLVNKDNKIIIQWDVIFKKLIDKK
                     EYFLYDNIIKIKTKNIDRYVNQYPSLLFNSSMDMLERKNKIDTKSIIKGKESAPKELS
                     EELDLNEEMKKVLYVKYLSANKMTLANLDSSVYFDVVLNDEYEPPEFEKFGIKPDDRY
                     DGFSLVAYHKDKKYILVPRIMVYCKRIDKEKWLYRNIDRIIKCAENIAAGIQDYSIIV
                     DELSLKDVTTDYYEEETEVIPPFYFERLDNNILEFSGIGKGREVARAYCSLDTSHNSF
                     EINLQKYELIIDGVSEVVEEEKTIAIGNAYQPVVSIMEKMPIISNNIKLAILKKDIED
                     LVIK"
     gene            complement(49921..50211)
                     /locus_tag="PKI50_01775"
     CDS             complement(49921..50211)
                     /locus_tag="PKI50_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT02478.1"
                     /translation="MKRYVIRSKSRFIVSVGIILIFAMSSLFTLAVSARENKDVTMIP
                     EYVEVGDTLWNMSQKHKGDMDIRKYISRVMEINGLQNANIKPGELLYFPGFK"
     gene            50377..50997
                     /gene="lexA"
                     /locus_tag="PKI50_01780"
     CDS             50377..50997
                     /gene="lexA"
                     /locus_tag="PKI50_01780"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNT02479.1"
                     /translation="MNIDDLSKKQFEVLNFIKKEINKKGYPPSVREICDAVKLKSTST
                     VHGHLERLERKGYIRRDPTKPRAIEVLDSNSSIVTKKDMVELPVVGKVTAGLPILAEE
                     NIEDTFPVPIDFVGNSEAFMLIVKGDSMINAGIFDGDFVIVRKQSVARNGDIVVALLN
                     DEATVKTFYKEKDYFRLQPENPYLMPIIAKELSILGKVIGVFRSIK"
     gene            complement(51045..51566)
                     /locus_tag="PKI50_01785"
                     /pseudo
     CDS             complement(51045..51566)
                     /locus_tag="PKI50_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
     gene            complement(51573..52811)
                     /locus_tag="PKI50_01790"
     CDS             complement(51573..52811)
                     /locus_tag="PKI50_01790"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT02480.1"
                     /translation="MKKKAIWIGVILLIAIIAGLKIKMGSPAVTVELEKVTRGDIEEY
                     VEETGILMLEQEAAVYSAASGRVIQVSKKEGDPVKAGEVIAKIDNSDIKLQIKALEAQ
                     KLGIEAKYAEAKTSADDETIRILNAQVRSAEAVYEEAKRTMDSNKVLYEAGAISLGTY
                     KNSLTNFAAAEAGLETAKSNLALAEKGISGNIKKQYEAQLSEINARIEQLKLKSEEMI
                     VTSPIEGLVITAEAEEGSIVQPGSKLFEIGGSEGYYIESNVLIEDIAGVSVGSKVIID
                     DEEIGMQDIKGTVREIHPKAESIMSDLGIEQKRVKVEISLDNEAAGLRPGYEMTVKII
                     VKSKKDTLLIDEKAVFSYKGKDHVFVNEGGTAKLRAIEKGIESSEQVEVLRGLTEEEE
                     IILSPDETLKEGTKVRSVPD"
     gene            complement(52824..55184)
                     /locus_tag="PKI50_01795"
     CDS             complement(52824..55184)
                     /locus_tag="PKI50_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT02481.1"
                     /translation="MVMRKLDVRLLRMVRHSKGQFVSVTVIVAVALCIYILFSITSTN
                     LRNSVDYYYKITNISDLTIQLVKIPQGALDNLQSIEGVRDVQGRVCVDVALEVEEEKE
                     RVKIRLISIPDNGGSINKLYSEQKVSMKLEDNSSVILLEQFAKARNIKIGDVIYPRIN
                     GRIHSLKVSGIASSSEFVYLMENEQSLMPAPEKFGVAYTSEAFAQSVFGYRGSYNEVL
                     VTTETDADIDDVIDRIEQKLDKYGVKKIIKLEDQLSNDVLEQELDGIEMLSSVLPVMF
                     LTVAAIIISIMLSRIVNNDRMAIGVLKALGYGNRIVLSHYIKYALAIGLAGAILGIWG
                     GMMLAGPISRYYTVFFNIPLAGVKIQYGYIFYGILLTCMFCTVSGLLGARRVIRIMPA
                     DSMRPEAPKSGKRTFIEKVTFFWKHVPFSWKMVIRNILRNKKRFAFLVLGLAMAYGIN
                     TVPLYMKDAMMSMFELQYGEYQKMDYTIQFNGPQNERIIVDLNNLIEVRRIEPRIEYP
                     FELAKDWRKMTVSVIGVPRQTAFHKFIDMKDNVIILPESGIFITEAIAEKLNAKKGDT
                     ITIKSFIPGKDDIETKVTGIFKQYLGANAYMNIESMEELLLEEQLITGVSVAAQADLK
                     DELKDVANISAVNSVDDMYRAYEEYIDLMLLAIGFYMLFGGVLGFALIYNSTIIGISE
                     RTMEFATLRIMGFDRKDVYGMICRENFVMGGIAIIVGIPLGAGMIKGMVESFSSDIMT
                     LPYILKPESFIQAAAATIMFVIIAQLATLRKVYNLNFIDALKSRIS"
     gene            complement(55178..55888)
                     /locus_tag="PKI50_01800"
     CDS             complement(55178..55888)
                     /locus_tag="PKI50_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02482.1"
                     /translation="MQKKILMKIENSSKDYKMGEVTVNALCDASFEIYEGEFIAILGP
                     SGSGKSTLLNIIGGMDLPTQGKVFLRGDEINKYNDRKLTAYRRDKVGFVFQFYNLMAN
                     LTAKENVELATEMCKEALNIDEVMESVELGDRKDHFPSQLSGGEQQRVAIARAVAKNP
                     LMLLCDEPTGALDYKTGLLILSLLHKINRQLKKTVVIITHNMAIGDMADRIIKMRSGR
                     IVEIKENASPIPPERIEW"
     gene            complement(55839..56474)
                     /locus_tag="PKI50_01805"
     CDS             complement(55839..56474)
                     /locus_tag="PKI50_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT02483.1"
                     /translation="MNRQTEKKLNNLLDKSKELFLKYGYNAITVDRIANQAGISKMTI
                     YKYFNSKEDLFMEVIRKDIEYQANEIMKAISESGSSIERIEILYNYSTNMAKNYSLVL
                     IRDIMEKKSIVDKVTEIKKNFMLPIFEFIISEGIRNEEIRELDMGFISELLMNLPKAL
                     LNIDFPSDEGKMIKFYGNFIDFLKFGLFGGKEGVKSAEEDIDENRKFKQGL"
     gene            complement(56657..58582)
                     /locus_tag="PKI50_01810"
     CDS             complement(56657..58582)
                     /locus_tag="PKI50_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNT02484.1"
                     /translation="MTSLSDLIKNDILYVINPKEYQREELINLLKDHPEIRFVSLAGA
                     DLTGNSTDERIPVRAFIEDIEEFLNGSVQTDGSSVVLPGIATLNDGKLDLVADTNSLW
                     FVDYNYENIDYETGRPVGTLRIPSFLMHGNKYVDSRSILIRGINRFRSEIMKILEKHP
                     SVCKEYGISCGDIDEILLTSATELEFWVMTPNDKADVEALTASQELQESYWKRTKGIV
                     RTSLEQSLILMDKYNLKPEMGHKEVGGIKAKLDETGNLTHIMEQLEIDWRYDTALQAA
                     DNEKWVRYIVKEVFRKNGLDVTFNAKPIEGVAGNGEHTHIGVAARLKNGKTVNLFTPS
                     DMKKDYLSIIGYGALMGLLKNYEAINPFISFTNDAFNRLKPGFEAPVCIVCSLGHNVE
                     TPSRNRTVLAGLVRNMSNPLATRFEVRSPNPKTNTYLALASIYQSMLDGIKAACESGK
                     TNSELEKEISKAPESEGFYLEKGRAYRSEEDVFEYYSQEERNRLFGVHPATVYENIKN
                     LAEYPEKLSVILEGNVLDERLVKSFTTAVMNRWVTEIINRLLPENMDTVRKCRAVHSK
                     QKAQDLDITYWAQVDHLRHYLMKDTYNSKSLFTRVREAAKEKEYETLSQLQKEMAEKM
                     SELKELYNDYKKNLIDI"
     gene            complement(58748..60043)
                     /locus_tag="PKI50_01815"
     CDS             complement(58748..60043)
                     /locus_tag="PKI50_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HNT02485.1"
                     /translation="MHYKTTDYLINKLNISKKIIDICVSTEEEVSVQFKKIDEVSEFN
                     QYKVLAAMQRNRISEMHFNCSTGYGYGDVGREALEKVYAEVFACEDALVRPQIVSGTH
                     ALALCLFGVLRPGDELISVTGKPYDTLENIIGTKDKRPAGCLTELGVVYKQIELTEAG
                     KINYNEITKKISEKTKMILIQRSLGYSWRPSLTMEDIEYTIKFIKIMNPNIICLVDNC
                     YGEFLDFKEPTEAGADIIAGSLIKNPGGSIAPTGGYVCGRADLVEMAASRLVAPGIGK
                     ECGATLGINRLFFQGLFIAPHIVSEAVKGAVFCSRLFSKLGFEVNPDYNTKRSDIIQA
                     IKFNNRDALIAFCQGIQKGSPVDSFVDPEPWDMPGYNDQVIMAAGGFVQGASIELSAD
                     APMRPPYIAYLQGGVTFEHVKIGLIYALQNLAGKGCISI"
     gene            complement(60422..60679)
                     /gene="hfq"
                     /locus_tag="PKI50_01820"
     CDS             complement(60422..60679)
                     /gene="hfq"
                     /locus_tag="PKI50_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNT02486.1"
                     /translation="MNKVNINLQDLFLNQVRKEHITITIYLVNGFQLRGNVRGFDNYT
                     IVLDNEGKQQLVYKHAISTIIPVKPINFAFSDNKPTENTVK"
     gene            complement(60760..61701)
                     /gene="miaA"
                     /locus_tag="PKI50_01825"
     CDS             complement(60760..61701)
                     /gene="miaA"
                     /locus_tag="PKI50_01825"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNT02487.1"
                     /translation="MPKICILAGPTAVGKTEISLALARSLCGEIISADSAQVYKYMDI
                     GTAKLKEEEMQGIKHYMIDEVTPDMDFSVAQFREKAELYIKDINDRSKLPIITGGTGL
                     YINSLLNNLDFTDSISDEEYRREMQETAQVKGTDYVHAMLEAVDPASYKKLHPNDLRR
                     VIRALEVYKHTGRPISYFQEKSRKQPPRYDFAYITLTMDRRKLYERIDQRVDRMMASG
                     LVEEVEGLIKRGYGRELNSMQALGYKEIADFLHGLITKDEAVRIMKRDTRHYAKRQLT
                     WFRGDKRVNWVDVDNFFRKEVIVENIIRFIAGKIPLI"
     gene            complement(61722..63488)
                     /gene="mutL"
                     /locus_tag="PKI50_01830"
     CDS             complement(61722..63488)
                     /gene="mutL"
                     /locus_tag="PKI50_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNT02488.1"
                     /translation="MGKIHILDEATINKIAAGEVIERPASIVKELVENSIDAGAAAVS
                     IEIQDGGKSFINVSDNGCGIMKEDTEQVFFRHATSKIKDSADIQGISTLGFRGEAMAS
                     IAAVSEVELLTKAEEDAAGSHVIIKGGKLVQSKSIGYPTGTSVTVRNLFYNTPARLKF
                     LKSDTSEQGAIVDIIEKLAMTNTGISMKLTVNNKVVLHTPGNGDLLSVILCIYGKSVA
                     GAMLPLEYSNDIISIEGYIGKPEIARGNSTYMTFSVNKRYVKNRMMSEAVKQAYKTLL
                     MNNRYPFSIININIKSEMTDVNVHPTKQEVKFSDERAIFNTLYLAVKSCLNNNRLVFE
                     NLEDVTEPQTAPSAAFESRNSYEPEKVSFDQLSFGREAAAPRADSYKWQYDYDRKTKV
                     QQKQFDNEIRVIGQLFGTYILGQKDDDFYIIDQHAAHERIMYEYIKDKYNEKNISVQE
                     LMMPIVIELSPAEKVLYAENSLIFQNLGFEIEWFGENTLAIRAVPILMGEPCTGEFFS
                     NILDSLKESAPNASSPLEKIIISMACKNSIKAGDTITPEEISVLLERLMKTCQPYTCP
                     HGRPTIITMSRYEMEKKFKRII"
     gene            complement(63500..66100)
                     /gene="mutS"
                     /locus_tag="PKI50_01835"
     CDS             complement(63500..66100)
                     /gene="mutS"
                     /locus_tag="PKI50_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT02489.1"
                     /translation="MAQFTPMMKQYFEIKNQHKDKILFFRLGDFYEMFFEDAITASKE
                     LEITLTGRDCGQEERAPMCGIPYHAAESYIARLIEKNYKVAICEQVEDPALAKGIVRR
                     EVIKIITPGTVIESSMLDERVNNYLLSLYADGRQAALSYTDLSTGEFYCTYADISDAA
                     VVRNEINRINPAEIISNTDFQDIKAAYSAYNILDKRYFDHEACERRLKRQFGVSSLEG
                     LGIDNETLLRSTGALLLYLDDIQKVSLDNINYIKTYSISNFMVLDQSTRRNLELSETI
                     RGKSRKGALLSVIDRTSTAMGGRKLKSWLEKPLLSTVEINGRLDAVAELYDDFLKREE
                     IKEYLKSMYDVERLATRIALGSANGKDLIAFKNSIRNLPYIKSLLSDCTSILLKDIYG
                     KLDVLEDLFGIIDASINEDPPFVLREGNLIKDGYLPELDEIRSASVNGKQWIAELEQA
                     EKEKTGIKSLKIGFNKVFGYYIDITKSNLPSVPEYFIRKQTLSNSERYITPELKRLED
                     LVLGSEEKLIELEYQTFIQIRNEIAGHIKRIQQTASCLSDLDCLCSLAEVSQKYNYVR
                     PEVNNEDRIIIKNGRHPVVEAQDNELKFVPNDTQLDNDENRMLIITGPNMAGKSTYMR
                     QVALITLMAQIGCFVPASKAEIGVVDRIFTRVGASDDLASGQSTFMVEMSELANIINN
                     ATSKSLIILDEIGRGTSTFDGLSIAWSVVEYIADKARLGAKTLFATHYHELTELEDKV
                     EGVKNYCILVEEKGKDIVFLRKIARGGAGGSYGIQVARLAGIPEAVLERSSEIISILQ
                     KSEKNSHTDVYVRRRRRNPVINDHEPNLFNFASFSLAEELKSVDLVNMTPVQALNKLQ
                     ELQDKAKDMI"
     gene            complement(66100..67545)
                     /gene="miaB"
                     /locus_tag="PKI50_01840"
     CDS             complement(66100..67545)
                     /gene="miaB"
                     /locus_tag="PKI50_01840"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNT02490.1"
                     /translation="MSERKAPEKVNNAEIERQKQIALRVRENNDIEKLKTGKQKRMHV
                     ITFGCQQNNSDSEKIMGMLAEMGYGKTDIKEEADVIIINTCCVRENAELKLYGNIGAL
                     KKLKEDNPSLIIGVCGCMMQQEHAVEMIRKKYRHVDIIFGTHNLYKLPELLERSINEK
                     YTLIDIMNTEGMIIEDIPVLREEKFKAWVTIMYGCNNFCSYCIVPHVRGRERSRKPGD
                     IIREIESLAKDGCKEVTLLGQNVNSYGKNLNMEMDFADIVRMVNEIEGIERIRFMTSH
                     PKDISDKLIYTMRDCKKVCEQLHLPFQAGSDKILKRMNRKYTKEEYLNKVFKAKQEIP
                     GVALSTDVIVGFPGESDEDFEETLDVLRRVEFDQVFMFIYSRRIGTSAAEMEDTSGYE
                     DKHRNFEKLVELQNGISKKLNDEYMDKIVEVLAEGSSKNDPSKFTGRTRTGKVVNFTG
                     TDGIIGKLVNVRINEIHSWFLNGQMVDEVKK"
     gene            complement(67775..68410)
                     /locus_tag="PKI50_01845"
     CDS             complement(67775..68410)
                     /locus_tag="PKI50_01845"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNT02491.1"
                     /translation="MLIVKGVLKHLTPKQRKIAEYWGDGPATKQWTPIIDRLLDTYGF
                     SPVRAARVLAAVQAGINDAFVITWYFKYLWDVPRPNQLDQRLVTALCTPKFPSYPSGH
                     SVISGTAEVILSYFFPPEAERLKELAEENSISRLYAGVHFIEDITEGLRLGRQIGRII
                     VEELMRQSDENQSGIDIPITESRNADLLPPPYEQVIAYPSRVKDCDLPLLP"
     gene            complement(68643..69110)
                     /locus_tag="PKI50_01850"
     CDS             complement(68643..69110)
                     /locus_tag="PKI50_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNT02492.1"
                     /translation="MKAEYINSFYKATKEVFKLMVGIDPQKKDLRVIEDMVSSKDASV
                     VLGVTGDLRGTILFGFPKDMTLEMIRIMSGMEMDRIDSFASSALGEVANIISGNAMTL
                     LAQNNYACDIVPPQIFIGEYKSFTMAGERALQLILTTPIGEFDINISLKEKQV"
     gene            complement(69110..69529)
                     /locus_tag="PKI50_01855"
     CDS             complement(69110..69529)
                     /locus_tag="PKI50_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNT02493.1"
                     /translation="MMWKDKYSIGIELIDEQHKELFKRVSDFLRTVESKDNWEDKLEK
                     VKETMAFMQEYVIVHFDDEEAYQEQIGYPEIENHKIAHLKFKEGINDYVRIFEEEGFN
                     EEKIQEFGGKLMAWLIMHVGKMDQMIGEYAKSKGGEA"
     gene            complement(69700..70407)
                     /locus_tag="PKI50_01860"
     CDS             complement(69700..70407)
                     /locus_tag="PKI50_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02494.1"
                     /translation="MLKVKDLHVYYGGIHALKGINLEVNEGEIVSIIGSNGAGKSTLL
                     NAISGFLKYKQGEISYNDNKLGFNPSKIVKTGICQVPEGRLVFANLTVKDNLYMGAYL
                     RNDKNEIKEDLEKVYSLFPRLKERENQVAGTLSGGEQQMLAMGRGLMSSPQIMLLDEP
                     SLGLAPLLVQTVFDIILDIRKMGKTILLVEQNAYKALSIADRAYVLEQGKISASGQAS
                     DIASDPKIQEAYLGVKK"
     gene            complement(70410..71174)
                     /locus_tag="PKI50_01865"
     CDS             complement(70410..71174)
                     /locus_tag="PKI50_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02495.1"
                     /translation="MGILELHGLTKTFGGLTAVGDVDFNVEKDSITGLIGPNGAGKTT
                     IFNLVTGVYKVTSGKIIFDDKDITNKEPYLIAESGITRTFQNIRLFKKLTVYENVLTA
                     CHYNANYSLLDSVLRTPKFRMQEKKLNEQAAELIAIMGLEDRKDLIANNLPYGLQRRL
                     EIARALALMPKLLLLDEPAAGMNPDETEQLMKLISEIRKKFNLTILIIEHHMDLIMGV
                     CENIMVLNFGCKLAEGTPKDVQNDKSVIEAYLGVEV"
     gene            complement(71176..72072)
                     /locus_tag="PKI50_01870"
     CDS             complement(71176..72072)
                     /locus_tag="PKI50_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT02496.1"
                     /translation="MGNEYILGILTLAGINLMTVLGLSLLTGFTGLFSFGHAGFMAIG
                     GYATALMTVKFHWPFIPALLAGGLAAALVSAALGKVTLNLKGDYFCIATLGFGEAIRL
                     ILDNVQTFGGARGWPGIPHYTNVWNVLGINIVAVIVLANLIRSRHGRNMIAVREEELA
                     SQIVGINVFNYKMTSLVTSAAYAGIAGGLMGHYMTFLQPKMFSLAKSTELTIIVIFGG
                     LGSISGSVLGALVLTSLPEILRAFSSWRLVFYGLAVIFIMISRPEGLMGGYELTPSSM
                     KKLFRNMAAKRENKLQRAGGGK"
     gene            complement(72085..72969)
                     /locus_tag="PKI50_01875"
     CDS             complement(72085..72969)
                     /locus_tag="PKI50_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT02497.1"
                     /translation="MTQFFQQLINGLSIGSVYALMAVGYSLVYSIMNFSNFAHGGVIM
                     FGAYFGFFFLTFLRFPFPLAFISAAICSAILALIIERVAYKPLRERRAPFLYFIISAM
                     GASIFMENLVIAIPAIGPTFRTYPEVFNKQPIKIGELAMGKLDIAMFLISAICLIILI
                     YIIDHTKIGKAIQATSYNLKASALMGVNTDFILITVFLMGGFLAGIAGVLFGMKYTVY
                     PQIGIITNKSFIAAVFGGLGSLPGAVIGSVLLGIIETFTAGYLSSQYRDLIAFVLLIT
                     VLVVRPMGLMGKVTEDKA"
     gene            complement(73081..74262)
                     /locus_tag="PKI50_01880"
     CDS             complement(73081..74262)
                     /locus_tag="PKI50_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT02498.1"
                     /translation="MKKKVLALLLVCLMAVSVGLAGCSPQTAAPAEPAAPAEEKILVG
                     WMGSLTGDQAVWGQCELNTVKMLFEEINAKGGVLGKQLEVIGYDTKGDPQEAVNVAKR
                     LTTQDKVCTILGPNASGNAIPIAPVLEEAKVPDIATVATNPKVTVLDGKIKPYNFRVC
                     FIDPYQGAVAAGYAADVLKAKTAAVLYDVGDDYSQGLTEFFEEFFVKKGGTIVAKEGF
                     KTGDVDFRPQLSKIKAANPEVIFMPYFFKEVALSSNQARELGINATLMGGDGWPSEQL
                     ISMAKDAVEGSFFVNHLDYDDPEVQDFKTRYKAKYEKDVELNGYLVHDAVLMFVDALT
                     RAGEANGEKIAQALETTDIKGITGRIKISKDTHNPEGKDAAILKIVDGKYVFQQKFAA
                     E"
     gene            complement(74644..75234)
                     /locus_tag="PKI50_01885"
     CDS             complement(74644..75234)
                     /locus_tag="PKI50_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02499.1"
                     /translation="MPSTFRNEELVKVQGKIYPVVGGRLRLAHDENENLSIETSVVQY
                     NEESTVVQAAVKTGKGIFNGLGNASVKRDKVLSNAILELAETRAIARALRFAGYGVEY
                     TGFEEVGEGKSISEENQSQQAEEHKNYEGATKTQINTIFAIAKNLNISNDELRDLIQD
                     KTGKSFSKDLSKSDASKIIRMLKERDVVENQGTKLM"
     gene            complement(75290..76903)
                     /locus_tag="PKI50_01890"
     CDS             complement(75290..76903)
                     /locus_tag="PKI50_01890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT02500.1"
                     /translation="MRLTFCGAAGTVTGSCHLIETGNKKILLDTGMFQGGSEAEEMNF
                     EPFRFNPSEIDMLILSHAHIDHSGRIPLLVKQGFKGKIISTAPTFELCKIMLPDSAYI
                     QESEAEWKSRKRKRAGKGVAEPLYTVKDAEASLKFFQPVEYNKIINIDPDISFRIMDA
                     GHILGSGIIELWIREGSEEIKLVYSGDIGNKDAPIMNDPTIIEEADYIIMESTYGDRL
                     HMEMNNKTLALLDIITDTIERNGNVVIPSFAVERTQEILYILNMLKECKSLPLCNVPV
                     FVDSPLAINATNIFQSFLPYMDEDTQKLLKEGDNPFDFPNLIFTHTAEESKAINSMEG
                     SSIIISASGMCEAGRIKHHLKHNLWREESSVVFVGYQAKNTLGRRIKDGEKLVKIFGE
                     EISVNCNVYSIEGFSSHADQRGLIEWVGSFIKKPKKVFLVHGEEESSKVLSKLMESEL
                     GIPVEAAKLGETLELSSAAAREITVERRSMNKHNLISYLRALKAASGDTFDSLEYIIS
                     EGSNTNNDSLLYHLKKLEESIAELKKEIS"
     gene            77093..77169
                     /locus_tag="PKI50_01895"
     tRNA            77093..77169
                     /locus_tag="PKI50_01895"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77127..77129,aa:Pro,seq:cgg)
     gene            complement(77176..78264)
                     /locus_tag="PKI50_01900"
     CDS             complement(77176..78264)
                     /locus_tag="PKI50_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HNT02501.1"
                     /translation="MLSQLESKCTSDIDQKEMRTLAVVLNHYDFRVESIDKVRSAYKV
                     GTDKGFFCLKRVNNGYEKAKKSYNIMLHLKENGCDYIAEYYFTKEGKPIIKRKSTAFY
                     LTYWIEGCEASFSIPDEMERYSELLADFHNRAKGFRTPKHVKVKSHIKKWRRTFAKCR
                     DELAGYREYIDKLKLKAEFDYIYKSNIDYFIREAELAIRILDHSRYNELCEYYVNEGS
                     ICHDSFYYQNIIVDRNNKLFVVDLESCQYDIPMSDLGKMIRRILSKKRFKWDFDLCRR
                     LIASYCKLRPMSKEEYEMLLAILVFPHKFWKLGRKRYIANKKWNEKKYLKKLKRQLRN
                     REYKREFIYCYINFYGLDLDYDPDIIEL"
     gene            complement(78397..79179)
                     /locus_tag="PKI50_01905"
     CDS             complement(78397..79179)
                     /locus_tag="PKI50_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNT02502.1"
                     /translation="MLENYEGFKQKILKLTGIDLSSYKERQMRRRIDSLIRRNNYDDY
                     DEYFKALTQNSNLYDEFINYLTINVSEFYRNPEQWKVLENEIMPEIMKFNKKPKIWSA
                     ACSTGEEPYSLAMMMSRYVDLNSFRILATDIDDAALNKAMVGIYSAKSLENLPRNFVD
                     SFFIKSGDYYKVSERIINCIDFRHHNLLVDKYPENCDLIVCRNVMIYFTEEAKITMYH
                     NFRNSLSNNGVLFVGSTEQIILPYKYKLESIKTFFYKKSGQP"
     gene            complement(79412..81709)
                     /locus_tag="PKI50_01910"
     CDS             complement(79412..81709)
                     /locus_tag="PKI50_01910"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HNT02503.1"
                     /translation="MKIIIDKYAGFCFGVKRAVDKAFEEADKKKDRRIFTFGPLIHND
                     QVTGKLEELGIKSADSIDEINCEGAELIIRSHGIGEEEYRQIEEKGFGYIDCTCPYVS
                     AIHKKVQEYYKKGYKIFIAGDKAHPEVIGINGWCDNSAHVIEDEKTVDKLPIYDKICL
                     VAQTTITKKIWDSVVDAINKKYKNAIIFNTICSATEKRQESAEQLSKKVNCMIVIGSR
                     KSSNTNKLYQICLENCDNAYHIEKAEDLPAGMLKGFETVGITAGASTPDWLIKEVIEK
                     MQDNGNIMGDPVEIMENQESVVDDGEEMEKQEDTTEDQGNMMEEYEKTMRPVYPGDTV
                     KGTVIMVTDDEVMVNIGYKSDGIIKKEDYTWDTDVSLKDIIKAGDELEVMVVRINDGE
                     GNVVLSKKLLDTEKNFNKIEAAFNEKTPLEGFVKEIVKGGGIVELMGVRAFMPASQFD
                     VRYSEDLSSFVNKKVNVEIIEFDPQKRKVVVSRKSILKKQNEIKEKALWENIEVGQKL
                     TGEIKRLADFGAFADIGGVDGLIHISELSWSRVKHPSEIVKPGDKVEVIVLSVDKEKK
                     KISLGLKQTVPEPWSAAGEKYKPGDVLDVKVLRFSNFGAFVELEPGIDGLIHISQISD
                     KRIAKPSDALKIGEIVKAKIVDIKPEEKKISLSIRELIEAIGEEAKEEAEKPVIEEAK
                     EETEKPVIEEAKEEAAEKPIIEEVKEEAEKPAVEEAKEEAEKSVIEEVKQEAEEAAEK
                     PIAEEAEEAAAENITEETAEETAEE"
     gene            complement(81693..82292)
                     /locus_tag="PKI50_01915"
     CDS             complement(81693..82292)
                     /locus_tag="PKI50_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT02504.1"
                     /translation="MFYRFARSLLIPVFLLFYNYRITGRENIPKDGPYIVCGNHVSAV
                     DPILVGISIPNRMYFMAKVELFRNRLLRALMNALGAFPIKRGEADIKSIKTSLKLLNS
                     GKILALFPEGTRKKSDGITAEPGIAMLAIKSKVPIVPVAIISSYKFFKRTNIIIGEPI
                     ELAEFYDKKLLNDEYIKISVDIMKRINELKKDRENENNY"
     gene            complement(82312..82959)
                     /gene="cmk"
                     /locus_tag="PKI50_01920"
     CDS             complement(82312..82959)
                     /gene="cmk"
                     /locus_tag="PKI50_01920"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNT02505.1"
                     /translation="MSGYKVIAIDGPAGAGKSTIAKKLADMIGYTYIDSGAMYRAITL
                     KVLRENIPLKKTDMLISMAEKTDIDFRDNSIYLDGRAVNTEIREEDVNRNVSYIAAIS
                     EIRKLLVDIQRKISMNKNVVMDGRDVGTVIFPEADVKFYITASVEERADRRYKELKQK
                     GYEAEIQDIRSQIEKRDYIDSTRSDSPLKAASDAIIIDTTGKGIDEVLSEVVSYI"
     gene            complement(83065..83523)
                     /locus_tag="PKI50_01925"
     CDS             complement(83065..83523)
                     /locus_tag="PKI50_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02506.1"
                     /translation="MNSSFQSWWDRIDGLIRKVLLLLFAALIISQALLLSQTIKNIIS
                     RIDKLEGRSIADSQLFIKRGELEISIENYSTLKPLVFYINGKGIDTPNGRSIRLKVKD
                     SDIIEVGGGPLNDTAVLKVTAVSDSITVPEPGKLVYVKDNLVMVDRIRIK"
     gene            complement(83588..84841)
                     /locus_tag="PKI50_01930"
     CDS             complement(83588..84841)
                     /locus_tag="PKI50_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT02507.1"
                     /translation="MVERMFFLIYDVIVVGGGPAGMIAAGTAASKGKRTMLAEKNEKL
                     GKKLFITGKGRCNVTNTADFDEFMKNIPKNSKFFFSSFSSFSNNDLILLLESLGLKTK
                     VERGGRVFPESDKSNDVIKALERYLNRNMVDVRLNSKLLDIRQENGAVTGALFDSGRV
                     VECSSIIICTGGLSYPNTGSTGDGYKIAVKAGHSIIEPRPSLVPLVSDDAFVKELQGL
                     SLKNVAIKAYAGGKLLYEDFGEMLFTHYGLSGPIILSASFFISDYLRRKQEIRISIDL
                     KPALSEEELDKRIIRDFEKNINKQFKNSLDQLLPQKLIPVVISRSGIDESKEVHQITK
                     QERKDMAKLLKGFTVTVSGTRPIEEAIVTSGGINLKEINPRTMESKLLKGLHFAGEII
                     DLDAFTGGFNLQIAFSTGYAAGFFA"
     gene            complement(84880..85506)
                     /locus_tag="PKI50_01935"
     CDS             complement(84880..85506)
                     /locus_tag="PKI50_01935"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNT02508.1"
                     /translation="MTRLYLVRHGETEWNKNGKVQGRTDTDLSPEGVIQAKLLARRLA
                     REDIDVIYSSSLKRALKTAEIIAEYKQCEIIESDKYHEICFGPWEGMTINEIKDKYGE
                     HFRVYKEDPVNFKLPGAETLIDLTERTYGAVMDIVTRHKGSNILLVSHGTAIKAVIIR
                     ILGLDIADFKKFMIDNASLSILEFPENSSGKPVVICLNDTGHLEEENL"
BASE COUNT        25693 a        17733 c        17224 g        24959 t
ORIGIN      
        1 ctgtgcatga atataggaat gggtgctgct gttttaacgg ctgagatata tttatccaaa
       61 ctgtttaaga atattcttca gaggggctga aataatgaaa gcaaaaaggt aattttgaga
      121 aaaggggtaa ggcggaatga aagcggtaat attaaacggg ggtaaagaga atgacttaag
      181 tattactgaa gtattaggca ctattgtgaa ggaacttgaa gcaaccggtg ctgatattag
      241 gacgtttgat ctcagggaca tgaatatatc gcgctgtttg ggctgcttta actgctgggt
      301 taagacaccc ggagtttgta taattgatga tgacggacga atgattgcaa aagaagcagt
      361 tcaaagcgat ttggttattt tcctgactcc gattgttttt ggcgggtatt caccggaatt
      421 gaaaatggca ttagacaggc tgatcccgat tgtttctcca tttttcacga aaatacaggg
      481 ggaagttcac cataaaccgc ggtataaaca ttacccttcc cttgtaggga taggaatact
      541 tccggaaaat gatcaaaaca aggaaaaaat atttaaaacc cttgtaaaca aaaatgcggt
      601 caatatgcac aatccatcat gggcagcatg tgttttctca gggagtcagc ctgctgaaca
      661 aatacggcag caaataaaag agtcccttgc gaaagtaggt gttattgaat gaaccctaaa
      721 aaagctgtat tatttatagg aagccccaaa gaaagaagcg catcggaagc ccttggagcg
      781 tatctgcttg gtaagttaaa ggagaaaggc ctgagtaccg ataaaattaa aatcgacaag
      841 ctggtcagca cagagagagg acaacttgaa ttatttgatg cagttaatca ggctgacata
      901 attatttttt cctgcccgct ttatgtcgat agccctccgg ctccagtaat aaaagctatg
      961 gaactaatca cggagaatcg aaaagggaaa ggcaaagaga acggaaaaaa gtttctggct
     1021 atttcaaatt gcgggtttcc ggaagcattt cataatcata ccgctttgga aatatataaa
     1081 gagtttgcct cagaggccgg tttcaaatgg ctgggaggac ttgcattagg gggcggcccg
     1141 gctgttcaaa gcagtgaatt aacaaagatt ggcggaatgg ccaaaaacat aatgagatcc
     1201 cttgatacgg cagcaaatga cattgttgaa aaagaggcat tgagtcatga agctgttgag
     1261 ctaatagcaa ggcccctggt tcccgcttgg atgtatttgt ttattgccgg attcggatgg
     1321 aaaatccaag ccaaaaaaca cggggtaagc cggagtatta atgactgccc gtatgagtaa
     1381 ttttttgaat atgtcttagg caaggaggtt tttatgaaaa gcattgattt caattttgtg
     1441 gctgatttat atgatgccta tgtcactgta gactttgaca aagatttcta tagaaaacta
     1501 tcaaaggaat gcaatggaaa atgcctttaa ttcattttcg gaaataaccg gcagcgcaag
     1561 gcaggaactg gctcttgaga atatctgcaa acacctggat gacggaggaa catttgtttg
     1621 caccttatat aatacggatt attaagggta ctgggaagct ttcagatcga tgaaaacaaa
     1681 agcttagtga tttcatatta taatgtctat aacggaaaaa ccaaaaatat aaccggtata
     1741 caattttatg agatatacga caaagataat aaattaaagg aaaaacgctg cctggatatt
     1801 tgtttttcac ttataagcaa agaagatttc atagagatgg cttcaaaagc cggctttaaa
     1861 ataaaagaaa ttttcggcga ctataatttt tctccgttct cggaagacag catgttcatg
     1921 aactttatat tgaataagga acagaagggg tgagatattg gaaagaacgc ttgtaattat
     1981 taagccggat gttatggaga gaaaattgat gggaaaaatt atctccattt atgagcagga
     2041 aggccttcac atatcctcaa taaaaatact gaagccttcc gtagaaaccg cagagtctca
     2101 ttattcggaa cataaaggca aaagcttctt cataagatta attgagtaca ttacaagggg
     2161 agaagtatgt gtattaatcg cagaagggga taatgccata gatagggcgc gaaaaataaa
     2221 cggggcgaca gatccggcgg aagcagacaa gggaagcata agaggcagat atggggtatc
     2281 aaaaactgaa aactccgttc atgcttcaga cagtgcggaa agcgcagatc gggaaataag
     2341 catctggttc ccggagctat aaatttgttc aaaggatggt attgagatga aatcgtttcg
     2401 aaaagaactg tactttaaac tgcctgtacg caggggctat gtaaacataa ccaatcaaat
     2461 acaagcctgc ataaatgaaa gcggaataaa ggaggggttt gtactggtaa atcctatgca
     2521 tatcaccgca agcgtattta tcaatgacga tgaatcgggg ctgcaccagg attttgaaaa
     2581 atggctggag gccctggccc ctgaaaagcc ccatagccaa tacaggcata acggttttga
     2641 ggataatgcc gacgcacatc tgaaacgtca attgatgggc cgtgaggtgg tggcagccgt
     2701 taccgaagga aagcttgatt tcggcacatg ggaacagata ttctacgggg agtatgacgg
     2761 caaacgtgaa aaaagagtgc tggtcaagat aataggtgaa taaaacattc aaaaatcggc
     2821 ataaaagtga tactattagt aatatataga gtgaaaggcc aaaattaaat aaggggggat
     2881 aactcagtga agactttgat agtatatgat tctgtttttg gcaacactga aaagattgcc
     2941 gaagcaatcg gcgattcact tgcggagttg acaggcactg aggttgagaa ggtaagcgaa
     3001 gcaaagcttg aacagcttca gggaacagat ttgctgattg tcggttcacc caccagagca
     3061 ttcaagccga caaaggcaat agttgatttt ctgattcaga ttccttccaa cggactcaaa
     3121 ggaatcaacg ttgctgcatt tgataccagg atcagtacgg aagatgtgaa ttcccggatt
     3181 cttaacggat ttgtaaagat cttcggttat gcggccaaac ccatagccga taagcttcag
     3241 aagaagggcg ggaacctggt agcccctccg gaaggttttt ttgtaaagga taatgaaggg
     3301 cccctgaagg aaggagaact tgaacgtgcc gccaaatggg caaaacaagt tatgaaggga
     3361 gaatgaggat gcaagataca tcagaattaa gatttgcggt acttatagat gcggagaata
     3421 tcccctacaa gaatataagg gggattcttg cagaggtagc caaatacggc actcctaccc
     3481 tgaagcgtat atacggtgat tggaccaatt cttcactgtc aggctggaag agtaacttat
     3541 tggagaatgc aataacacca atccagcagt acagctatac cacagggaaa aattcctccg
     3601 actcggcatt gattattgat gcaatggata tactgtattc cgagaaagtt gacggcttct
     3661 gcatagtatc aagcgacagc gactttacaa ggctcgctac aaggcttcgt gaagccggta
     3721 tgaaagtaat cggaataggg gaaaggaaaa cccccagcgc atttattgca gcctgcgata
     3781 aattcatata tattgagata ataaatgcac ctgtcaagaa cagcgatcaa gacagcaatg
     3841 agctgcacag ggatgatacc gttgaacagg atcataaaaa ttctctggat atcgggaatg
     3901 atattataag gctgatatcc gattccgtaa atgacgtagc cgatgacaac ggctgggctt
     3961 tcctggcaga tgtgggcaac cttatctcaa agaagcagcc tgactttgat gccagaaact
     4021 atggcttttt caagctgact ccactggtta aaagtctcaa atgctttcag atcgaagaga
     4081 gggagtcggg taattcaaga atcaagcata tatatatccg gaataagaac aagcagtaat
     4141 tcacatattt ccgggaagca gcagaaggga taatatgaga acttacaaga gaatgtatat
     4201 aaaactgatg ctatccgtaa cagcagtatt gggtatctgt tttattgccc ttgacttctc
     4261 aaggtttttg aatcaaaact acagctatga catatttccc ggtgcaattc ttaagagaat
     4321 atgcgtaatt atagctgccg taattgcatg gtcagtggga agggatgaaa taaacccgaa
     4381 ggacagcaga ttgatgaagg ccgcctttat atttgttatc ttgggagaag cagccttttt
     4441 tatcagggaa agagcaatcg ggatagggat gtttgccctg tgtcaaacct tgctgactgt
     4501 aagaaatggt acaggcctaa gatataaact ggcccgggca ggcagtaatc aaaaaatagg
     4561 gcttattgtc tcaggtataa tagttattat ttccgtaata tttccgtttc tgaccggtta
     4621 cttgttcaag acggatatta cattgaatat agcctattta tacggaataa ttttgagtat
     4681 atcattatgg gcggggcttg cctgcaatat actgggtttg cttcctcaga gaaacgccgg
     4741 gatggccgct ttgggaatga catgcttctt ttgctgcgat gtcctggtgg ggcttgatgc
     4801 cgtattggag gcaggattgc catggctgct ggcaaacagc ttcatatgga tattttacat
     4861 tcctgccctt gtattgcttg ctttaagctg ttacagatat aattaattta aggagtatca
     4921 taatgaaagg taccatatat gcaattgcag caggaatatt tattgccatg cagcaggtat
     4981 tcaatgcaag attgggagaa aaagcgggtt tatgggaaac aaacaccttt gtacacggaa
     5041 gcgggtttgt attttcatta ataatattaa tgattgccgg aaacggaagc tttgccaaaa
     5101 ttggagagac gaacagactg tatcttctgg gaggggtatt gggagtcctt atagtattca
     5161 gcgcgatgat tgcagtttcg gctctcggtg cgacatacag catagcaata ctgttggtta
     5221 cacagcttat tgcggcaact gtcattgatt ccttcggcct gttcggcagc cctgttattg
     5281 aatttgatat aaccaaactc ctgggaatag caattatgat tgcaggcata attgttttta
     5341 agcttaaagg atgattatta gaattccatg cggcagtgag gccgctcagt ttgcagacaa
     5401 agctcaaaat aggaacagta atgtatagtc agagcatact tatacaaaaa ttgttaatgg
     5461 cccttatgag gctcgactta gaaacaaccg ttatcggatg ttttttatta gtctcagcgt
     5521 cgagagacgc ttttttttat gccgctttgg ggccattgaa gtattttgga cttcctgcct
     5581 gaatattaat tattatacca actataatat tttattttgg agggatagtc ttgaaatcca
     5641 ggataataat aatcagtaag ttaaaacttt tagcgatgct tctaattata gccgccctgc
     5701 tgctttttat cttcgcattg agaaccatca gcataaatac tcttcattat tatgacccaa
     5761 tatataaagg aatggaagat gataaaaaaa ttgcttttgc ctgcaatgtg gtatggggag
     5821 aggaatacct gccgcaaatg ctaaagctgc tcgaagaaaa ggatatagaa atcaccttct
     5881 ttatcggagg gcaatgggct gagaaaaatg aagaacttct gcgaaacatg cataaagccg
     5941 gacatgagtt gggaaatcac ggctacaagc atttatacca cagcaagctt actcctgaat
     6001 cgaacaagca ggaaatatca aagacggagg agattattaa gagaataaca ggatacaaaa
     6061 cagtcctatt tgcccctccc tacggtgata taaacggtac tgtggtaagc tcggcggaag
     6121 ccttgggtta caaggtcatt atgtggagca tagatactat agactggaat accagggatt
     6181 acaaaaagat attgcagcgg gtggaaaaca agcatcataa aggagctatt gtactcatgc
     6241 atccgactaa ggttaccgtt gccgcactgc cccaattgat tgagaacctg gcaagtcacg
     6301 gctatgaaat aaccacggta agtgatgtat tagaaaacga ttgaaaaaat accgtatatt
     6361 atatataatg agatgtacat ttgaaaaaaa ctaaaaaacc cggaggtaaa ttaatgtatc
     6421 tgaaagagac ccttaaaaat ggaataagaa tagttgctga ggaaatacca tatgtcaatt
     6481 ccgtttccat cggtttatgg tttaaggtgg gatcacgata tgagagcatt gagaataacg
     6541 gtatttccca ttttattgag catatgcttt ttaaaggcac aaaaaacaga tcggcaaagg
     6601 aaatagcaaa cagcatagat aaactcggag gccagcttaa tgcgtttaca tcaaaggaat
     6661 ccacatgctt atatgcgaaa gtgctggata ctcatttcga tatagctttg gatgttcttg
     6721 cggatatgtt tttcaattcc aactttgact gcgatgaaat cgataaggag agaggagtag
     6781 taattgaaga aataaacatg tatgaggatt ccccggagga tcttgtccat gacctcttca
     6841 gccagtcggt atggtcggga aaccctttgg gaatgtccat tctcggaact gaggaatccc
     6901 ttgaaaatct taacagagat ataatagtcc gatattttaa agaaaactat tcccctaaga
     6961 atattgtcat ttccgttgtg ggtaatttca agcataagga tgcttttgac aaaataaagg
     7021 catattttga gaaagccgaa ctgattaata ggtccgctac cgatattgca gtgccccgct
     7081 ttgagcccga gtatacactt aagagcaagg ataccgagca ggtacatctt tgtatgggct
     7141 tcaacggtgt tgatattaag aacaaggcat tttatcccat gctggtttta aataatgttt
     7201 tcggcggagc gatgagttca aggctcttcc aaaaaatcag ggaggacagg gggcttgcat
     7261 attcagtttt ctcatatcct tcttcctttg aggactgcgg actgttctcc atttatgccg
     7321 gttcaaagcc tgataatctt aaaagcgttg cgaagcttat aatggaagaa ataaacatga
     7381 tcaaggacaa gggcattacg gaggaagagc tttatgattc aaaagaacag cttaaaggca
     7441 gttatatact tggccttgaa agcaccagcg gaaggatgat ttccataggc aaatcagagc
     7501 ttttgcttga cgtaatatat tctcctgcag agatattggc aaaaatagac aaggttgata
     7561 tggacaacgt gaacagtacc ataaagcaca tatttgacac ggacagcatg ggggctgctg
     7621 taataggaaa catgaaaaac ggcactgata taaggaatct tttcaaatag gtgaaggatg
     7681 tgaagcaaag tgaaggatgt aacagtaaaa tacatcagga caagcccgga tgaaaacccg
     7741 gaaccaaaga tacccgcata tacaacggaa ggctctgccg gtatggatct gagcgcgtgc
     7801 atttccgagg atatcatcat aaaacccggg gaaattgcaa tggtgaaaac aggaatagca
     7861 atacagattc ctgacagcag cattggaggc tttgtatttc cgagaagcgg cctttccagc
     7921 aaacacggga tttcccttgc caactgtgtc ggcgtaattg attccgatta taccggcgag
     7981 ataatatgcc ccgtaatcaa tcacggaagc agtgactata cgataaaaaa cggagacagg
     8041 atagcacaga tagtatttat gcctgtagtg aatgtacggc ttcaaaaggc ggacaagctg
     8101 gaagccacgg gaagaggagc gggaggcttc ggctcaacag gcaagtaaat taaacaggaa
     8161 tattcgtccg cttcattaat ataattaact atagaattat tgatggggtg gtactatgag
     8221 attcagcaag ctgggcggga aggagattgt ttcccttcct gactgtgaaa ggctgggcta
     8281 tttgggcgac tgtgacctta tgattgacgg ggaaaccggc aggataaaat ctcttatagt
     8341 tcctgaaaac aaagggacat tatcgttttt tatagacaga agatatatag agatcccatg
     8401 ggaaagaata aaaaaaatag gcaacgatat gataataata gattttgcgg atattttaaa
     8461 atagaaataa gagaaatggt aggggacatg gggacgtttc tcctgtcccg ctttcagcgg
     8521 gacaggagaa acgtccccat gtccccttgt aagacacgcg aaacgtcccc gtgtcttgcc
     8581 catgtccttg tgttttatgc gctttggata atcaccaact gcatataaat ctcatattat
     8641 aaattagtta tatgtttgga ggaatctgta tgaaaagctc aagatacctt attatcggcg
     8701 gtgatgacag gcttgtcgag cttgcaaagc tttttgaagg ggaaggggct gacatcgcca
     8761 cttatgggat ggacatgact gtaataaaga atgtaccaaa ttatttatcc tttgatgaag
     8821 cgctggaaaa ctcggatata gttatcgggc cgataccctt tgccaaggac gtgcacaaaa
     8881 ttaattcaaa gtattccagt gttgatattg aagctgaaga gctgttcagt aagctgggaa
     8941 aggataagaa gctgatattg ggtgcaataa acaattattc aaaggggctt gctgaaaaat
     9001 acggcatcga atactgggac taccataatg atgaaggata tcagatacta aacgccatcc
     9061 cgacggcaga aggcgctctg tcaatactgg ttaacgagac gaaaacgaca ttgttcggaa
     9121 gcagaattct catactgggc tatggccgta taggcaagct gctttccgaa tatctcaaag
     9181 cattaggtgc atcgttatat gtagaagcga gaaaggattc tgaccttagc tggataactg
     9241 cagagaggat gaaagccata ccccttaaag agcttcctca atatctgggt gaaatggaaa
     9301 taataatcaa taccgtacct gcaatgctgc tggacagcaa tatgcttgat ttggtgaagc
     9361 aggaagctct catacttgat ctggcttcag cgcccggagg aactgatttt gactgcgcct
     9421 caaaaaaagg cataaaagca atacatgccc agggaatccc gggtaaagcc gcatgtaaat
     9481 cagcagccga atatatttac agcacaataa aaaaatattt atgtgctgat cggcagaaag
     9541 gagtttaata atgaaagttg aaggcttgaa tataggattg gcagttaccg gatccttttg
     9601 cacctttgat aaaataattc ccgaggcaga aaagctaatt aagcaaaaag ccaatgtata
     9661 cccgatattt tcaacaaatg ctgcttcaat ggacaccaga ttcggaaaag cggaggactg
     9721 gcttaggcga tttgaggaaa ttaccggcaa cagtgtgata aaaaccatag aagatgctga
     9781 acccataggc cccgggaaca tgttggatgt tttagtaata gcaccctgta cagggaatgg
     9841 ttttacaatt tcaaaactac gttgacatcg cccttatatg cttctatcct ttttatcggg
     9901 agttcaggca gctgcctgtg cattgacggc tccttttttc ccgtaatcgg ctcaattgct
     9961 tcaagaaccg cttttgtaag tttttcggca ttcacataat ttgaacaatt gcggcatctc
    10021 agataaatat actttttgcc ctttgaggaa gcatgaaagc tggtaagcgg cattccgcac
    10081 ttcatgcaaa aggcttttaa ttgcatcccc gaataatcgg atgctttccg tgaagttctc
    10141 tttctcgttc cggtacttgc tttttgagct ttattccaca gctcatcatc aattattgca
    10201 tcatgggttc ccttgacgct gtactggttt tccttgccta tcaagtgcct gttgataccg
    10261 atgtagaccg tattgtgcag gatatatgat atgctgctgc ctctgaattc acagcctctc
    10321 ttactcttta accccatgct gttcagatat cctgctatgg cggaacagtt catgccgcct
    10381 tcagcatata acctgaatat caaccggact attcctgctt cctcttcatt tacaacgagt
    10441 tttttgtcaa tattgtcata acccagtacc gtagatgatt tccctccctt tcttgcaaac
    10501 tccttaagcc ccagctttac attctcaaca atagtattcc gctcaaattc tgcaactgag
    10561 ccaagaagct gcaatgtcat gcggccaatc ggtgttgatg tgtcaaactt ttctgagcag
    10621 ctggcaaaac atacaccatt attttcaaaa atatctatga gatataacag gtctttaaga
    10681 tttctggaca gcctggagat tttccagacc agcacaatat caaaaaaacg gcttttacaa
    10741 tcttcaataa gccgcttcag ctcatgcctg tccttcatac tcttgccgga aacacccaga
    10801 tccatataat aatcatatat cgatataccg aaagcaatgc aatattgctt aagggtttca
    10861 gcctgtgcat ctgcggattg gccatccaga gcctgttctt cagtactgac cctgatataa
    10921 agcgcagccc tcaaactttc cttgtctgcc atttgctcac cttacatttt tctgcttagt
    10981 aaattatatt aacactcatt gaataataag cacctaaaaa attgaaaaaa ctgcggcata
    11041 aaattccgca ctttaagcat gttgtacccg gagggcagaa ttgctataat ttagtagaaa
    11101 gagttatatt attacttata aaaggagaag ccctatgagt attatcgata cctttctgtt
    11161 aagatacgcc aaggaatatg atttttacaa cgaattggcc catcaggtgg cgatgatttg
    11221 tgaaaccata atacacagaa gcggaatcag agctattgta acctatcgcg cgaaaaagcc
    11281 cgaaagcctc aaggataagc tgatgaaaag gaatgccatc aaaaaatatc agagcatcga
    11341 acagatatac cgggatatag tggatctttc cggagtgaga atagcaattt attttcccgg
    11401 cgacagggag gaaataggga ggcttattga aaacgaattc attaccagaa aaacgaaaaa
    11461 attcccgaat tccgagcaga agcagcatca ggataatccg ttcaaaaggc gtttcacggg
    11521 ctatgatgcc gttcattaca gagtaaagat aaaagaggaa aagctggagg agaacaataa
    11581 gagattcgca cacgcccagg tagagataca gatagcatca gcccttatgc acgcatgggc
    11641 ggaagtggaa catgaccttg cctacaagcc tcagatagga aagctttccg aggaggaatt
    11701 caacatattg gatgagctta acggccttgt actttcgggg gatataactt tggaaaggct
    11761 tcaaaagtca ttcaaacaaa ggatagcctc aatggagcag caattcaaca atcattacga
    11821 gcttgccgcc cttttgaggg aaaagatatc cggcagcttt ccggaaggct gcttcatcgg
    11881 cagagttgat attctgctga aatttcttca aaagataggc atggataatc cgaaggaaat
    11941 acatatgtac ctggattctt ttgacaaaat gaaggaatcc gtaggatcca aaaccatagt
    12001 ggattgtttt gcggatctta tattgaatca ttaccccgat tactatagac agtatgttga
    12061 agcaaaattt gaatcaagct tcaataatcc gtacatatcc atccaaaaac agatgcttct
    12121 cggtaaagag aatgagcagc tcaattcctt cgttgaaaaa tggatacttt ttgaaaatgc
    12181 tataaaggct ttggaacaaa aaaacataac gcagagccga agtatttccg atattataag
    12241 aaggcacctg actgctgatg aaattatact tgatactgtc aagaatctgg agaaaaccag
    12301 gaatcatata atatacagca gtgatatacc ggataattta caattaagaa atgctgcgga
    12361 ctcaataaca ttaatactga acaagcttgc ggaactggct tcggatgaga ataaaacagt
    12421 tatttgcaag gctttggata aaataatcaa agagttttag ctgcaaactt attccctaca
    12481 ggcaatactt tatcaaaact ggcaaatgcg ataactgata cgcctgtgtt aatggcggca
    12541 aaggctcatg taagaaacgg caagccaatt gtagtaggga tttccacaaa cgacgcactg
    12601 ggtttgaatg cgaagaacct ggctgtactg ctgaactcaa agtattacta ctttatcccc
    12661 ttctggcagg ataatcctgc tgcaaaaaga aattctatga ttgccgatat ggagcttttg
    12721 ataccaactg ttgaaatggc attggaaggg aagcagcttc agccaatgat aattgagaaa
    12781 tctaaataga gaggaacaat cacatgaaga taattgtaca aaaatttggc ggaacctcag
    12841 tatcaactca ggaaagaaga gaactggtgg ttgacaaaat agaaagtgca gtgaaaaaag
    12901 ggtttttgcc tattgttgtt gtttctgcaa taggaagaaa aggtgacccc tatgccacag
    12961 atacccttat aaaccttgca gcttccgcag gagtcaaaaa caatcccaga gagctggata
    13021 tgattatgta ctgcggtgag ataatatcct gtgttgtact gtctaataca attaaaaaaa
    13081 gaggctatga caatatagta ctgacgggcg ggcaatccgg aataataact gatgagaaat
    13141 tcggtgacgc cgaggtctta agagtggagc cccattatat aaaagaatgc attgaaaagg
    13201 ggctcatacc tgttattgca ggctttcagg gcatatcgga gaagggtgaa ataacaactc
    13261 tcggaagagg cggaagcgat gtcacaggtg cgataatcgg tgaagccctt aatgctgagg
    13321 cggttgaaat atatacggat gttgaaggcg tcatgacggc ggaccccaaa attgttccgg
    13381 atgctgcggt aattggtacc atatattaca acgaagtttt tcaaatggcg gaatacggtg
    13441 caagagtaat tcatcccaga gcggtagagg ttgcaatgag aagcaatctg ccgatattta
    13501 tcaagaacac aatgtcagac tcgtcaggca cattgattac caattgtgat aaaaaccgaa
    13561 gatacagata tgataagaat gataaattgc tgacggctgt ggcccatgta ggcggaagaa
    13621 cgagagtaag aatactgttt gacggttcgg caaaggaata tgagaatgac agccatctgt
    13681 tcagtacaat cgcttcggag ggaatcagca tcgatatgat taatgttaat cctgatttta
    13741 aattatttat tattgttgat gatgatgttg aaaagcttgg aacagtgctg aaccgatgcg
    13801 gatataacta tgagctgaaa aaggattgca gcaaggtgac gataatcgga aacaaaatgc
    13861 gcggggttcc gggtgtaatg gcaaaagcaa taagggccct ggcaaaacac aatattgaga
    13921 tcctgcagac atccgactcc catacgacta tttcctgcct gatagaaagc ataaatacaa
    13981 atactgccgt aaatgcactg catcatgagt ttgagctggg aaaaccggca attcagaacc
    14041 tgtgaggcat ctttttcccg ttataaatga attcctctgt ttccaaagga tatcggagga
    14101 attctttttt atgccggttt gtgctaatat attaatgtat tgaaataatc gggaatttct
    14161 aaaaaaagtg aaacaaaaca ggaaaatatt agtctaataa taggatgcac tgacaagcgc
    14221 agttttttat ggaagagtgg gtaatatgga taaaatcata cagctcaaaa atatcagaaa
    14281 agtatacaga atgggaaacg aaaaggtcat agccctggac aacataagct ttgaaattga
    14341 agaaaaagaa atctgctgcc tgttaggcac atccggctcg ggcaaatcga cccttctgaa
    14401 tcttatggca ggccttgaaa aaccaacaaa gggagaagtg aaaataaaga actataacct
    14461 tgaaagcctg aatgaaaaac aactggcaaa gcttcggcaa aaatacatag gctttgtttt
    14521 ccaatcctat aatctgcttg aacactttac tgctttggaa aacgttactt tgccgctgat
    14581 attcaaagga gtgcccaagt ctgtaagaga gaaaaaggct aaggccttgc tgaaggctgt
    14641 cggcctgggt gaccggatga atcacaagcc ctcacaaatg agcggtgggc agcagcagcg
    14701 tgtaagtatc gcaagagcct ttatctcaaa tcctgagata gtctatgctg atgagcctac
    14761 gggtaacctg gataccagaa cctccaccga aacaatggac ctgataaccg gaatggccga
    14821 ggaatacaag caaacactta ttatcgtgac tcatgacttg gaaatagccg ggtatgcaaa
    14881 ccgaatcata tatataaagg acggaaacgt agaaaaaata gatatgctga gtgaaaaggc
    14941 gggaaggtgt taatatgaaa aggaagttat ttattgtttt tttagctgcc attatagcat
    15001 ttgcgggtct gcctgctata aatgtatcgg caggtattta tgatgatatt gaagtatcat
    15061 acgatgttaa tgaatcaagg atattggacg gtgatagttt tacactatat tttactattg
    15121 atgaggtgcg tgcaggtgaa ataagtgatg ttgaaattaa tattgaaacc ggaagtaatt
    15181 tcaaagtact gggagggaat cttaatattc ccgctgctgt tctggataaa caatacaatg
    15241 ttaatttgca gtatcttgga ggaagcaaca gccttccgat aaaaataaaa tacgacaaaa
    15301 attctgtata tgagggggag tttatctata atgtagaaat tgcagagata gatgaagaga
    15361 gtgtcaatcc gtcctcaagc agctccgaca acgagcccaa gctgattgta gaaagctctt
    15421 ccattcctgt ggctgctgcc ggtgaaacca tgaaaatacc cattacagta acaaatgaaa
    15481 gccggtattc tgctaaagat gtcactgtta aactttcact cgcaaacaat gacaataatc
    15541 cctttgaaat aagcagtctc aacctggtac aatctatcgg cagtttaaat gcaaagaaaa
    15601 gcgacgggtt gatcttcaga ctcaaagtaa acccatacgc agaagaaaag acctatgaat
    15661 tagatgtaca atttagttac tataactcaa agaacaactt atttaccagc aatgagaaaa
    15721 tatatgtaaa ggttaccaac agcaagaatg agccggtgct tatgatccag gatataaagc
    15781 acagcacata tcctgtaaat gccggggcaa aattccagat aagctttaat gcggccaatg
    15841 taggaggtat tgacgcaaga ggtgttaagg tttcaatcga aggcctgaac aaagacggat
    15901 ttaccctgta cagtggtgtt aacagctggt atttcgacaa tatagaaggc aacgggaaaa
    15961 agaatattac tgtggatctt gtagctgctg actctatgga aagcggaaat tacggcctgg
    16021 aaatcaagat ggagtataag gatcaaagga acaacagcta tactgccagc tcaagctttt
    16081 ttgtcactgt caaaggagaa gccaaaagct caaatgtgac aataaaaaat atctccgtat
    16141 tgccgcagga agtcagggca aacgagaact ttacgttaag cttggatgtt cttaatgaag
    16201 gcaaatcctc tgccaggaat gtcaaattga caataaaggg agacgagggc ataatatcca
    16261 aatctccgga tgtaagaatc attgagaacc tgcctgcggg agaacagcag aagctggaat
    16321 acgtgctcta tgccaataat gaaacgaaaa ctcagaatta taacataatg ataaaccttg
    16381 aatatgaaga tgcggataag gagaattcaa agcattccgt taatcagtat gcgggagtat
    16441 atgttaacgg gggaaacagc aagattactc ccaaaataat aataagcaac tataattttg
    16501 aaccgaagct ggtcagggca ggcgaggaat tcagcatgga tctgaccttt atgaatacaa
    16561 gcagcacaaa ggaagtaaag aatataaaga tatatctgac gggaatagat tcggataaag
    16621 agggaaatgt ggtattcaca ccggtgggaa gcagcaatac atttttcgtt gatgccataa
    16681 agcccaaggg caccgctgta agaagcatta caatgtacac aattcccgat gcccagccca
    16741 agtactataa tataacggca aatatcgaat accaggatga tgaaggtacc gagtacaaag
    16801 ctgaggagct gatcggaatt cctgtaatac agcaatccaa gctggatgca tcggaaattg
    16861 ggttcccgcc ggaaacattc atcggccagc ccattccggt aagtcttcaa tattataata
    16921 aaggaaagac aaggctttcc aacctgatga taaagattga gggtgatttc cggatagaaa
    16981 acggagaagc cttcatagga aattttgaat cgggctccag tgactactat gaggcaatgg
    17041 ttacgccaat gaaacccggg cctgccaaag gaaaagtagt tttcagctat gaggatccat
    17101 caggtgatga gttcacctat gagagggaat tttccataaa tgcaattgaa atgcctccca
    17161 tggagatgcc cggcggagag atgcccatgc ctcctgtgag cactaaggat aaaataatga
    17221 aatatacaat taagaattgg tttttctggg tgtgtatcgc agtaatagct gcagggattt
    17281 ttaccgccag gaggctgatt aggaacaaga gacggaagga tatggatctg gatgaatagg
    17341 atagatatag taaggatagt ccttaaaaac cttctcagaa gaaaaatgag aacaatgctt
    17401 actgtaatgg gcgtagttat aggcactgct tcaattgtaa taatggtttc aatagggata
    17461 ggaatgaatg agggcttcaa gagtgaaata tccagaatgg gaagccttaa ttcaataaat
    17521 gtaaggtccc ctgaaacagg tatgtactat atttatgatg aaacagaggc gggaaggaca
    17581 aattctacgg aaatgcggga tttggatgat gctgcagtag cagatttttc aagaattgcc
    17641 ggtgttgaat cggtaattcc cttcattgag actcatggaa aaataaagct tggcaaatat
    17701 attggctatg tccagcttca gggaatcaat cccgagcata tggataaact tggtttgaag
    17761 gtctcggaag gaagactgct gggtgccggg gataaaatga atacagtatt tggtttcgga
    17821 gtaaaagata acttatataa ccctaagggg tcgcaaaggc gaagatttgg cagaaacaca
    17881 ccggaggtag acctcttggg tgaaagaatc actctttcat tggatatgtc ttatggagag
    17941 gaattcatcc agagagatac ttctgttcag aaaaagccca aaacatataa aataaacgga
    18001 gtcggtttgc ttgaagaggg aagctatgaa tactacagca gcatttttat ggatattacc
    18061 gagcttaaaa aaatagtaaa ggatttcaac aagggtaaga gtaagaatga taattcagga
    18121 gattaccggg acattaaggg ttattcaaga ataatagtga aagtgaaaga tataaataat
    18181 gtgcaaagaa taaaaaatca gataaaggat ttgggatttt ttgcagacag cctgaccgat
    18241 attctagaat caatgcaaaa aaccacatcc agcataagaa tcgttctcgg tgcaatcggt
    18301 gcagtatccc ttctggtcgc atccattgga attaccaata caatggtaat gtctatctat
    18361 gaacgcacaa gagagatagg tatcatgaag gtaatcggct cttctctggg agatataaaa
    18421 aggatgtttc tttttgaagc atcaatgata gggcttttag gaggactaat cggattgctg
    18481 ctgagctatg gagtatccta cgggcttaat acggcaggct ttgcattgat tggcttcttc
    18541 ggcccgtccg gaggaggaag caaaccctca attatacctt tatggcttgc agttagcgca
    18601 atgctgttta cgacaattat cggacttgtg tcgggatttt atcctgcgca ccgtgcaatg
    18661 aatctcagcg ctcttgaagc aataagaaaa gaatagccat gcagcccttc aaaaaatacg
    18721 acattgtcgt attttttatt ttattaaggt aaaaatatta tttaaatgta tagctgaatg
    18781 tacaatggct aacgaaaaaa atcgggagag tgatttcctt gacagaagca gatggagcag
    18841 ataaagaaaa agaggctaag aataatgcca atgaaaatat taaagaactg ggcgaagtca
    18901 gagtgccgca attcaggagc aatatacact gcattacaat aataggccag attgaagggc
    18961 atatggtgct tccgccgcaa aacaaatcaa caaaatatga acatataata ccgcagattg
    19021 tagccgtaga agagaaccct gagattgagg ggctgctgtt aattctaaat acagtaggag
    19081 gggatgtcga ggcgggactt gcattgtcag agcttatagt aagcatgaaa aaaccgtcag
    19141 tttctcttgt gcttggggga ggccacagca tcggagttcc cctggctgtt tctgcaacac
    19201 attcctacat tgcgtccaca gcgaccatga caatacatcc cataagaatg aacggcctgg
    19261 ttatcggcgt accgcagacc tttgactatt ttaataaaat gcaggagcga attatcagat
    19321 ttatagtgga gcacagcaat atagacgttg atacaataaa gaggctgatg tttacaacaa
    19381 gcgagctggc caacgatata gggacagtgc ttgtaggagc cgaagcagtt aaatacggcc
    19441 tgataaagga aataggaggg ctgcatcagg cacttgcaaa actgcagaac ctgatagcgg
    19501 cttctaaagc agcggaaaat caaaggagta attgaataca aaggattaga gaacattgta
    19561 acatttgtca aggatctgcc ggaaacgtca ggtccttaag ttttgcaata atacataagc
    19621 aaggttttgt tgcaaccgat tccaaataaa gctataataa cattaagttt aatatacttt
    19681 ttaagaggtg ataacttgtc agggaagaga aaagagaacg atttaaacta tgaaatatct
    19741 ggagtgtctc tgatattagc cagtatatac atatatataa gccttttcag cagcaattca
    19801 ggaattgtag gagaaaaagt caggttattt tttctgggaa ccttcggcct tggtgcttac
    19861 atagtgccgg tactcataat catactgggg ctggctttgg tatatagaaa gaatgaaatc
    19921 cgcattaata ttaagttttt ttctttctgt atatttttgc tgtccacact tatagtgctt
    19981 tttttgtcaa actatacaga gattgaaaat gcggatgcca aatggctcga tgctgtcaga
    20041 tactattttt ccctgggcat tctcaatgca gggggaggag cagtgggagc cttgttcggc
    20101 tatgcctttg taaggctttt tggaatgata ggcaccatag cgatagtttc gtcactctac
    20161 ataatatcaa tactgatgtt caccgaggta tcatccatgc gaatccttca gtatataaaa
    20221 caatccgcat cggatatcta ttcacgttta aggaacaaaa acaaagaagc cgcagtaaca
    20281 aaggaattgc agagaaaaat acccgtaaag catcacaacg atattgaatc atcagaagct
    20341 gacgatgaag aaagaataaa gaacatcgat aaaaaaataa agattcttga ctttacaaaa
    20401 cgtttcgata aggatttcaa gggcacggcg gagaatgaag ccgatgacaa agccaggaac
    20461 aaggataaaa caagcaggga aaacattcca agcatagtac ctgtcagcaa catacattac
    20521 gtgaattaca aacttcccgg caccggcctc cttgcattgc cggcggagaa taagtcaggc
    20581 aacgggaaga aagaggtaat gaacaatgta aagatacttg aggatactct gaaaaacttc
    20641 ggtgtcagtg ccagcattgc acaggtttct gtcggcccga cgattacaag atatgaactg
    20701 cagttaagcc cgggggtaaa ggtaagcaag atattgagcc ttgcggatga tatatctctc
    20761 agccttgcat cccccgatat aagaatagaa gcgcccatac ccggaaaagc agccattgga
    20821 atagaagtac ccaacaagga cataaccagc gtgcttttga gggaagtgct ggaatcggaa
    20881 gagttcagaa agcataaatc aaaaatatcc tttgcattgg gaaaagatgt agcaggggca
    20941 aatattgtgg cggaccttgc caaaatgcct catcttctga ttgccggatc taccggttca
    21001 ggtaaaagcg tgtgtataaa cacgcttatc gcaagcatat tgtataaagc aactcccgaa
    21061 gaagtaaaac tcctgcttat agaccccaaa gtggttgaac tcagcgtata caagaacatc
    21121 ccgcaccttt tggtgccggt ggtgacagat cctaaaaagg ccgcgggagc attgaactgg
    21181 gctgtaacgg aaatgaccga gcgttacaag aaatttgcgg caaataacgt aagggatata
    21241 tacggctata acaatctgaa caaggaagaa ctggccaaaa tgccgcaaat agttgtcgtt
    21301 atagatgaac tggccgattt gatgatggtt gcgccaagtg aagtggaaga ctctatctgc
    21361 agattggcgc agatggcccg tgccgccgga atacacctgg ttgtggctac tcaaaggccg
    21421 tctgttgatg taattacggg gcttatcaag gcaaacatct cctcaaggat atcctttgcg
    21481 gtatcatcac acattgattc aagaactata cttgatatgg gcggtgcaga aaagcttttg
    21541 ggaaaaggcg atgccctgtt ctatcctgtc ggagcggcaa aacccgtcag ggtacagtgt
    21601 gcctttgtat ccgagaagga aatagaaaag cttgtggcct atattaagga acaggttcct
    21661 gcaagctttg acaataaaat aattaaagaa atcgagcaaa gtgtagaaat tgatgaggac
    21721 gcagaagctg acgagctgtt gaatgatgca ataagaattg ttgtggaaaa cgggcaggcg
    21781 tcgatactca tgcttcagag aaggttaaga ataggctaca gcagggcggc aaggctgata
    21841 gaccagatgg aggaacgcag gataatcgga ggctatgaag gcagcaagcc cagaaaggtt
    21901 cttttgggca gagaggactt ggaagattag tttttggaag gagcgaattt gttgagtctt
    21961 aaagttgcaa tcatatccct tggatgctca aagaacctta ttgattcaga ggttatggtc
    22021 aagagcatcc ttaataatga ttatgaagtt acggataatg ccaatatcgc ggaggtaata
    22081 atcataaaca cctgcggttt catagaaagc gcaaagcagg aatccataga caccattctt
    22141 gaaatgtccg attacaagaa aaacggcaga tgcaaagtgc ttattgcaac aggctgcctt
    22201 gcggaacgct atagggatga gcttatcaaa gagcttcccg agttggatgc ggtaatcggc
    22261 accggagatt ataaggacgt tcctgaagtg ataagtaaag cactgaaggg tgagaaggta
    22321 gtcctatacg ggcatcagga actgattgat atagataagc ttccccggca catttcgggc
    22381 ttcggtgcat cctcgtacct taagatagcc gaaggctgcg acaacagatg cagctattgc
    22441 ataatacctg ccttgagggg aaggtaccgc agccgaaaaa cggaagacat acttgaggaa
    22501 gcaaaggagc tgtcaaggaa cggaataaag gaagtaaatg tcatagctca ggatataacc
    22561 aggtatggac ttgatttgta taaaagccgg cagttaagcg aattgttaaa aaagctggcg
    22621 gaaatcaatg gtatcgaatg gataaggctt ctttattcat atcctgatga attcaacgat
    22681 gaactcatag aagtcattgc aagagaagat aaaatctgca aataccttga tatccctata
    22741 cagcatacca gcaacaggat acttaaaaag atgggcagga gaagcagccg agagcaaata
    22801 caacggctga ttgaaaagct caggaaaaga ataccgggca tcgtacttag gacaactctt
    22861 atcgtgggat ttccgggaga gacggaggaa gatttcaaag agctgtatga ctttgtttca
    22921 gaaacgaagt tcaacaggct gggagtattt gcatattcaa gagaagaaga taccccggct
    22981 tttaatatgc cgaaccaggt tgaagaagcc gtaaagcagg aaaggctcga aaaaataatg
    23041 cttcttcaaa aggatatttc gctggaatac aaccggaagc ttgtcggtca aacactgaag
    23101 gtgcttgtgg aaggtgcttc cgataatgaa tactctgggc gaagctgcat ggatgcccct
    23161 gaaatagacg ggaaggttta ttttaaatcg aacaaggaaa ttatccccgg ggatttctgc
    23221 agcgtaacca taaaaaaggc atatgaatat gacttaatgg gggagagaac agatgaacct
    23281 ggcaaataaa ataacaatta tcagaatact tatgattccg gtttttttac tgtttgctgt
    23341 cgcagaattt ccttatcata tgacagccgc cgttgcggta tttcttgcag cctcgcttac
    23401 cgacaagctg gacggatata ttgcccgtaa atacaatatg attaccgact ttggcaaatt
    23461 catggatcct cttgcagaca aactgcttgt cacagccgct ttcttagtac ttatacagct
    23521 cggcaggata gaagcatgga ttgttttcat aatacttgca agggagtttg ccgtaaccgg
    23581 gctcagaacc ctggcagccg cccagaatat cattattgcc gccagcagct acgggaagat
    23641 aaaaacagta actcaaataa tagcaatagt aatactgctt atggataact atcccttcag
    23701 cctattaaat ctgcccgttg acgccgtaat gacctatgcc gcgcttttaa gtacagtgat
    23761 atccggaatt gattatttta agaagaatat gcaggttata acaagaatga aataatgtct
    23821 ctttaggagg tttttgcatg aaaagcgaaa ttattgcagt tggtacggag ttgcttttag
    23881 ggaatatcgt caacaccaat gcgcagtacc tgtcacagaa gctggcggat ctggggattg
    23941 acgtatacta tcatgtagtt gtgggagata atctgaaaag gctgaccgaa acaatcaaaa
    24001 caagccttga acgatcggat attgttatta caagcggcgg cttggggccg acagcagatg
    24061 acattacaaa agaaggggct gcacaggcta tgggtttgaa gctgctcccg gatgaagaaa
    24121 gcatagagag aataaaaaaa atgttcagtt caaccggaag aatcatgaca gaaaacaata
    24181 tcaagcaggg atatattcct gaaggcgccg ttgtacttga aaacaacaac ggcactgctc
    24241 cgggtgtgct gatagaaaaa gaagggaaaa tcgtaataat gctgccgggc ccgccgaaag
    24301 aattatatcc gatgtttgag agcaaagtgc tcccctatct caagtccaaa acagattcaa
    24361 caatcaggtc gaaagtactc cgggtgattg gagttggaga gtcagccgtt gagcacattc
    24421 tgaaggatat attcgacagc caggccaatc cgacaattgc tccctacgcc aaggacgggg
    24481 aagtacatct gcgaattact gcaaaaacag gtataccgga ggaagctgac agtcttattg
    24541 ctgagatgga acaaaaagta agagcaatac ttggtgacaa tatatacggc tgcaatgaag
    24601 aaacactgga agaggcggta ttgaagctgc ttcagaaaaa aaacctgact atttcactgg
    24661 cggaatcatg caccggaggg cttgtcgcaa gccgcctgac agatattccg ggagcctctg
    24721 caagtttaat ctccggagtc atcagttaca gtaatgaatc caaaataaac atgctaaagg
    24781 ttaaggaaga aaccataaga aaatacgggg ctgtgagccc tcagacggcg gaagaaatgg
    24841 ctgtcggcgc aaaaaggctc agcaatacgg atatcgggct ttccataacc ggtatagcag
    24901 ggcctgacgg aggaagcgtc gaaaagccgg tagggctttg ttatataggt atagcaatag
    24961 gcaaatccgt aaatgtgcaa aaaatcatgc tgaccggaaa caggaacaga ataagatggg
    25021 ggtcaagcag cagagccctt gactttctca gaagagaact tttatcagga taaaaacagt
    25081 aaaattatgt aattttcttg actaacaatt agcaatataa tataatagaa acataagaac
    25141 atttgttcga tttgcctcaa gggcataagt gatgaaagga cggtgcagga attaatgtgc
    25201 tcagcaatga acgataaaaa gaaagcaata gaactggctc tcagccaggt tgaaaagcaa
    25261 ttcggaaaag gctccattat gaagcttggc gatgccacaa aaatgaatgt tgaagctgtt
    25321 tccacaggct ctatagaact tgatatagca ttgggaatag gcggagttcc cagaggaaga
    25381 attgtcgaga tattcgggcc tgaatcctcg ggaaagacta ccgtagcgct gcatgttatt
    25441 gcagaagcac agaagaacgg cggtgaagca gcctttatag atgcggaaca cgcattggat
    25501 cccctatatg ccaagaatct tggagtggat atcgagaacc tgatagtatc ccagccggat
    25561 acaggggagc aagggcttga aatagcggaa acacttgtcc ggagcggagc aattgacgta
    25621 gttgttgttg actctgttgc ggcactggtt cccaaagcgg aaatagacgg tgaaatggga
    25681 gacgcacatg tgggactgca ggcaagactt atgtcacagg cactcagaaa gcttgccggc
    25741 gctatcagca aatccaggac tacagttata ttcatcaacc agcttagaga aaaggttggt
    25801 gttatgttcg gcaatcccga aacaactccc ggcggaaggg cgcttaaatt ttatgcttcg
    25861 gtcaggcttg aagtcaggaa gatagaaaac ataaagctcg gaaatgattt aaccggaagc
    25921 aggacaaggg taaaggttgt caagaataaa gtggcgcctc ccttcaagca ggctgagttc
    25981 gatatcatgt acggccaggg catttccaaa gagggcagta ttcttgatgc ggcggtcagc
    26041 aacgatctta tattgaaaag cggcgcatgg ttttcctatg gagatataag aataggacag
    26101 ggcagggaaa atgcaaagca gtatctcaag gataaccccg atatcacaaa ggaactggat
    26161 aagaagctca gggaaatgtt taacctggga atatccaaag caaaagccgc agacttcgat
    26221 tccgaagaaa acgatgaatt ctaatatgag ggtgtttcca tgagaatcac tgggattgaa
    26281 caacaaaaaa agaatacgaa gagatacaat atatatattg acggagaata caaatgcacc
    26341 attgaagata acatcctgca ggaactgaac ttgtcaaagg gtatggaact gaatgaaata
    26401 gagtttaata ataccctgga aataatccaa tataagggcg ctcttagagc tgcccttttt
    26461 atgcttgccc gcgcgccgaa aacagaaatg gaaattgaaa agggactcag cgacaagcag
    26521 catgccccaa aggccataaa aaaagttttg gaatatttga aggaaatagg ctatataaat
    26581 gatgaaagct acgccgaaag ctatataaga agcataaagg ctacaacagg cacaagccgc
    26641 cgaagcattt accaaaaact cgcagctaaa ggaatagaca aggaaatcat tcagcaaatg
    26701 cttaataagg ctgagataga tgattatgca tccgcaataa gtgccgcaaa gaaaaaggca
    26761 gcaagcctga agggaaacag aagggaaaag aaattaaagc ttcttaacta tctttacaga
    26821 aaaggctttg gaattgatga ctgcaaaagg gcggcagagg atatcttaca ggaagaagac
    26881 tgaaaggcct ccagcaaaat tcaagcaatt ctgcataaca tccttaaatt gtttgaattg
    26941 tcacaataac ttgacaaaat tttgtataag taatagaatt attctgatat ggtgtatcaa
    27001 ttgagagtga agcgtagttg ccattatttg ttaatgtgaa aatgatgatt atgaaaaagg
    27061 attaatggaa accgttaccg ataatcgata aaataaaata atgaggaggt gaaaatatta
    27121 tcgaatatgt aatatatata gcaattgcac taattacctt catttttggg tatttagtta
    27181 gaagatatat tgctgaatcg aaaataaaaa atgccgaaga gcaggcatta aaaatagtca
    27241 gtgaagctga aaagcaatcg gaaacaaaaa agaaagaagt tgttcttgag gctaaagaag
    27301 agattcacag acttagggca gaactggaca gagagattcg tgacagaaga aacgaaatcc
    27361 agagagcaga aagaagattg ctccaaaggg aagaaagcct ggacaaaaag gttgagaacc
    27421 tcgagcaaag agatgaggca ctagccaaaa agcaaaagga gattcagaaa tccttagagg
    27481 aagtacaaca gctttataag aaacaagtgg aagaactgga aagattatcg gggttatcat
    27541 ctgaagatgc aaaacaactg cttttaaaca atattgaaaa acaaataagg catgaagctg
    27601 ctatgatgat caaggatatt gagaccaagg ctaaagaaga agcagaaaga aaggccaagg
    27661 aagtcattgc aagcgcaatt cagaaatgtt cagcggacca tgtggctgaa actacagtat
    27721 ccgttgtacc gctgccaaat gatgaaatga agggcagaat aataggacga gaaggcagaa
    27781 atataagaac attggaaaca cttacaggca tcgacttgat aatcgatgat acaccagaag
    27841 ccgttatatt atcaggtttt gatccaatta gaagggaagt tgcaagggtt gcccttgaaa
    27901 agctcatagt ggatgggcga atacatcctg ccagaataga agaaatggtg gataaagcca
    27961 agaaggaagt agacaactat attaaggaac aaggagagca agctaccttt gatacaggag
    28021 tccacggatt gcatcctgaa atcattaaac ttttgggaag attgagattc agaaccagct
    28081 acggacaaaa tgtacttaag cattcaatag aagtagcaca ccttgcggca tcgatggctg
    28141 cagagcttgg tgccgatgta aaaacggcaa aaagagcggg tctgctgcat gacataggaa
    28201 aagccgtaga ccatgaagtc gaaggccctc acgttcagat tggtgccgac cttgctaaaa
    28261 aatataagga atccgcagat atagtacacg caatttccgc acatcacgga gatattgaag
    28321 ctacaaccgt tgaagcagtc cttgttcagg cagctgatgc catatctgct gcaagacccg
    28381 gagcaagaag agagaccttg gagtcataca ttaaacgctt ggaaaaaatc gaagagatag
    28441 ctaattcatt tgatggagtt gaaaaatctt ttgctattca agccggcaga gaagtcagaa
    28501 tcatggttaa gccggaggat gtaagcgaca gtgaaatagt atatatcgcc agggatatcg
    28561 tcaagaaaat tgaagatgag ctggaatacc ctggtcagat caaggtaaat gttatcaggg
    28621 aaaccagggc aattgaatac gcaaaataga aaatgtgcgg cgatacatgc aaatgcatga
    28681 agccgccatt tttaataata ataattaaaa acgggtaaca ctaaacacac ggctgtttca
    28741 cggattcctg cgcaataaca cagctgattc agacaatgga ggtatgcaga tgaacatatt
    28801 atttattggt gatgtagtag gcaggcccgg aagaaattat ctgagtacaa accttagcag
    28861 gcttgttgaa gaaaataata ctgattttgt cataataaac ggagaaaatt ccgcaggagg
    28921 agtaggcata accaggagta catatgacga gcttcttagt atgggagcgg atattataac
    28981 ccttggcaat catacctggg cgaaaaaaga agtgttggaa ttcatcgaag acgccgaaag
    29041 gcttatcagg cctgcaaact atcctgaaga catccccgga aaaggataca gaatactgga
    29101 aaaggcaggg aaaaggattg ctgtgataaa tctttgcggc agagtatata tggattgtat
    29161 tgactgcccc tttaaggcca ttgataaaat attgaatgaa ctgaagaata aagctgatat
    29221 cataatcgta gacttccatg gagaggctac ttctgaaaag cttgcaatgg gctggtatct
    29281 tgatggcagg gttcaagccg tgctgggtac gcacactcac gtccagactt ccgatgaaag
    29341 aatattgccc ggcggaactg cctatattac tgatgtcgga atgaccggcc ccatagattc
    29401 catactggga gtggataaag aagcagttat cagaaaattt attaccggta tgcctgcaaa
    29461 atttgaaatt acagggggtg aggttgctgt tggggctgta tgcatacagc ttgatgataa
    29521 taataaagtt acatccataa aaagattcac acaaaattct aaattgtaga aaaaaagagg
    29581 attttagata aatttgtaga attatcagat atatttatta tattttgtta cttttcaata
    29641 aatcaggggg gggctctata tggatgtatt aaaggtttca gcaaaatcga acccaaattc
    29701 agttgcaggt gctttatcgg gtgtgttgag agaaaaaggt tctgcggaga ttcaaacaat
    29761 aggagcgggc gctttgaacc aagcaattaa agcaattgcc attgccagag gatttgtagc
    29821 acccggagga attgacctga tatgcattcc ggctttcacc gatatcgaaa tagatggtga
    29881 agaaagaaca gcattaaaac taattataga acccagataa actcaatacc tgcttttaac
    29941 aagcaggtat tgtttttttt tgtataaaag tgtatacttt cctgtataat aattatggaa
    30001 catttttgga aaatcctttg tgtattgcat aaattctaaa gaatttatga tataataatt
    30061 gcagttcctg caattattat atctttttta ggaggcgcag tatgcaaaag gtagatctgc
    30121 atattcatac aacagcatcc gacgggctca tgagtccgga aaaagtagta cgctgggcaa
    30181 gctccaaaag attgactgcc ataggaataa cagaccatga cactgtcaag ggaatcggcc
    30241 ctgcagtgaa ggctgcttcc aggtatgggg tggaaatcgt gcccggaata gagcttagca
    30301 ctctatttga agaagaagag atacatgttc tgggttattt tattgattac aaatcccaat
    30361 ggcttgccga cactctcaaa aaaatacaga attccaggta tgagagggcc gggaaaatag
    30421 taggcaagct taacgatatg ggaattgata ttactcttga agaagtaaaa ggtatagctg
    30481 atgatggtgc aatgggcagg cctcatattg caagggcaat gattgataaa ggctatatca
    30541 gcaatatcag agaagccttt gccgaatata tcggcaagga tcgcccggct tatgtggaaa
    30601 gatacaaact aacctgcaaa gaagcaatag atatgataga aaaactggga ggaatatctg
    30661 ttcttgctca ccccgggctg ataaaaaata aggataacat cggaaaaatt attgaccttg
    30721 gaattgacgg cattgaagta taccattcca aacacgatga tgagacagta aaaaatatcc
    30781 tggctgttgc acattcgagg aatttattga ttacgggagg ctctgactgc cacggaatga
    30841 aatttaataa tgaaccgata ttgggaaact attcaattga ttataaatat gtacaaatgc
    30901 taaaagaggc attgaataaa atatgaggag gggtcaaaaa tggagataaa attatccgac
    30961 aaagtcttaa atatagctcc atccatgact ttggcagtta atgcaaagct taagcaaatg
    31021 gttgatgagg gtgttgaagt atttggcttt ggtgtaggtg agcctgattt tgatacgcct
    31081 gagtacataa gaaatgcagc tgttgaagca atgaacaaag gctttacaag atatactccg
    31141 gcacagggca caatcgatct cagaaaagca atatgtgcca agcttaaaag agagaacagc
    31201 ctggattatg agccgaatca gataatagta tcaagcggtg cgaagcattc gctttcaaat
    31261 gcatttgcgg caattttgaa tccgggggat gaggttattg tacctgttcc atattgggtc
    31321 agctacactg agataatcaa gctgaatgac ggagttccgg ttctggtaca gacaaaaaaa
    31381 gaaaacaact tcaagatgtc cggggaagag ctgaagaatg caataactgc aaagacaaaa
    31441 gcaatattga taaattcacc cagtaatcct acgggtgcag tgtacaccga agtggaatta
    31501 agggaaattg ccgaaattgc ggtacaaaac aatctgtttg ttgtatcgga tgagatatat
    31561 gaaagaatca tatatgatga taacaagcat atcagcattg cttccttcaa cagtgaaatc
    31621 aaagatctta caatactgat aaatggaatg tcaaagtcat atgccatgac gggctggagg
    31681 ctcggttatg ctgccggaaa tgaaaaattg attaaagcta tgtctagtgt tcagggtcat
    31741 gcagtatccc accccagttc cataacccaa tacgctgctg cagcagcctt aaacgggccc
    31801 caggatgatt tgaaaaacat ggttcaggaa tttaataaaa gaagaaacta tatgtatggc
    31861 aggataaatg caatgaaagg ccttagatgc ataaagcctg aaggagcctt ctatgtatat
    31921 gttgatataa gctcttatct gggaagaaat ctatgcggaa tcaagctgga ttcttgtttg
    31981 gattttgctc aggttatgct tgaaaagggg catgtggcgg tggtaccggg ggctgcattc
    32041 ggaactgaag gctttgtaag gatttcctat gcgacctcaa tggaaattat taaaaacggc
    32101 cttgacgcct ttgaaagctt tatctataaa aacattgaat agggtggtat catacagtgg
    32161 acaggaaaga atatcaaaac cctttgataa gcagatatgc aagcaaggaa atgtccttta
    32221 tcttttccga ggactataag ttttccacct ggagaaagct gtgggttgcc cttgccgaaa
    32281 gcgagatgga gctggggctg aatatttctc cggaacaggt ggaggaatta agagagcata
    32341 tttatgatat caattacgag gattcagaga aaagagaaaa ggagacgcgg catgatgtaa
    32401 tgtcccatgt ttatgcttac ggcttacagt gccccaaggc aaaacccata atacacctgg
    32461 gagccaccag cgccttcgta ggcgacaata ccgatgtcat tcagatgtcg gaaggcctca
    32521 ggctcataag aaccaagctt ctgaatgtta ttaaaaacct cgcaggcttt gcagaaaaat
    32581 ataaaaacca tcccactttg gggtttaccc attttcagcc tgcacaactt accactgtcg
    32641 gaaaaagaag ctgcttgtgg atgctcgatt ttctgatgga tatggaggag cttgattttc
    32701 agctgggcaa gctcaagctg aggggagtca aaggtactac cggtacacag gcaagcttta
    32761 tggcgctttt taacggtgac catgaaaaaa taaaggaatt ggacgcaaga gtcacaaaga
    32821 aaatgggctt tgagaaaagc tttgaagtta cgggccagat atacccgagg aagctggaca
    32881 gccaggtttt gaacgtgctt tcacaaatag ccgaatcggc gcataaattc agcaacgata
    32941 tacggctcct ccagagcctc aaggaaatcg aagagccctt tgaaaaagga cagataggct
    33001 cgtcggcaat ggcttataaa cgaaatccca tgagatgcga gaggatcgca gccctttcaa
    33061 gattcgtgat aacaaattcg ttgaatcctg caataactgc ggctgctcaa tggtttgaaa
    33121 gaacccttga tgattccgcc aataaaaggc ttgcaattcc gcagggtttt ctggcggttg
    33181 acagcatact gaacattatg ataaatgtta cggccggcct tgtagttaat gaaaagatga
    33241 taaaaaggca catcgacgag gaacttcctt tcatggctac cgagaacata cttatggaag
    33301 cagtgaaagc aggaggggac aggcaggaac tccatgaaaa aataaggcag ttctctatgg
    33361 aagcaggaaa aagagtgaag gaagagggcc tggacaacaa tctgataagc cttatagaag
    33421 gggatgaaag cttcaggttg ggcaagaata agatacagga gcttttggat gccggaaact
    33481 ttatcggaag agccccggaa caggttgaag agtttcttgc ggaacatgtt tatccgcttc
    33541 ttgaaaaaaa caaggataaa ttaaatatta cagcagactt aaaagtataa atataatcac
    33601 acattaggag gagagcatat gagcagcata cccataaaac aactgtacag gagcacggaa
    33661 gattacatca gcaagaatat caaggtatcc gggtgggtaa agactgaaag aacctcaaaa
    33721 aattttggtt ttattgaatt gaatgacgga agctttttta agaaccttca gattgtgttt
    33781 gaagaagacc ttgaaaactt caatgacata gccaaacttt cggtaggttc cgccataatc
    33841 gcagaaggat tgctgacgga gtctttaggc gcaaagcagc cctttgagct gaaagccgtt
    33901 agaattacaa tagaaggcaa atctacaccc gattatccgc ttcagaagaa gaatcattcc
    33961 tttgaattct taagaacgat agctcacctg aggccaagga caaatacatt ttccgcggta
    34021 ttcagagtga gatccctggt agcatatgca atacacaggt tttttcagga gcagggtttt
    34081 gtatatgttc atgcccctat cattacagga agcgatgccg aaggggcagg agaaatgttc
    34141 agggttacca ccctggatat agataatccc cccaggacag aaaaaggaga agttgacttc
    34201 tccagggatt tctttggaaa atccgcgaac cttaccgtaa gcggccagct taatgcagaa
    34261 accttttgca tggctttcag gaacgtatat accttcggcc ccaccttcag ggcagaaaac
    34321 tcaaataccg caaggcatgc cgcagaattt tggatgatag aaccggaaat agcctttgcg
    34381 gagctttcgg acaatatgga actggcggaa aatatggtaa agtacataat caattatgtt
    34441 cttgagaatg cgccggaaga gatggaattt ttcaacaggt tcattgacaa tactctgctc
    34501 gataggctga agaatattgt aaactccgat ttcggtattg ttacttatac ggaagccata
    34561 gacatactga aaaagtccgg tgtagatttt gaataccccg tcgagtgggg aatcgacctg
    34621 cagacggagc atgaaaggta tatcaccgag caggtcttta aaaaaccggt gttcgtaatc
    34681 aactatccga aggaaattaa agctttttat atgagaatga acgatgacgg aaaaacagta
    34741 gctgcaatgg accttctggt tcccggagtg ggagaaatca tcggaggcag ccagagagag
    34801 gaaaggcttg aggtacttga aaagaaaatg gatgaaatgg gactgaataa agaggattac
    34861 tggtggtatc ttgatataag aaaatacggc ggtgtcaaac atgccggcta tgggctgggt
    34921 tttgagagaa ttataatgta cataaccgga atgggaaata taagggatgt agtgcctttc
    34981 ccgagaacgg ttaaatcagc tgaattttaa tgggacgcgg ggacgcgggg acgtttctcc
    35041 cggggacatg gggacgtttc tcctgtcccc gtgtcctcgt gtcctattgt aactaaataa
    35101 gacaacgcct catattatgg aataagggtt catttcattc catatacctt tgaagggagg
    35161 cttaataatg ggtcgtaggc actcgtcggc atataatgca ttacctccgg ctgcatgacc
    35221 gccgtcatca tatccggcgg cttcgccggc acgaagcctg tttcccctgc ctttttccct
    35281 gtctgcgctt ccgacagggg aattgttggg aggaatcagc aacaccgtaa gagggtttgt
    35341 tttaaatgac aactggtggg ttatactgct tataattttt ttatttttcc ccaaggtcag
    35401 cggcaggatg tttggagggc ttcttggaaa atagactcct gagaacctga agttattcgg
    35461 ttttttggat ataaacagct attgactaag catgtttttt tttatataat gaataagtgt
    35521 cacaggggta tagctcaacc ggtagagtag cggtctccaa aaccgttggt tgtgggttca
    35581 agtcctactg cccctgccat tatatgaatc gtacagccgc aattgtttgc ggcttttttt
    35641 atttttgatt tatttccgtt ttattattcc actccgattg caattgtata acatgaaagc
    35701 tcaattgtat ttttatacaa aaatgagctt tttttttgaa ccttttgaac tcagcgtatc
    35761 ggctatgata tatcaaaaat gcccggagct tataagctgt atcttgcagt ggctctctac
    35821 aggcttctga attaatctcc ttttatatca ataaaaactt ttttgtgtta ctccccattt
    35881 tctcaaggtt tcatcattgc ctatccatac agatatacca accggtataa tattataaag
    35941 actcaggaca tctgaattta tcatgcgtat gcagcctttt gacacctggc gcccaatgga
    36001 ataggggctg ttatttccat gaataccgta caaattgcct tttccgacag aaaggcctat
    36061 ccatcttgtt ccaagagggt tcttcgggga accgccggct acgggttttg aaccgtcgcc
    36121 cggagaccat accgggttct ttcttttcag gcatacatgg aattttccct ggggggtttt
    36181 gtttgtagct tttcctattg ctatcggata ctttccaatg actgtttttc cgctgtacaa
    36241 agtaagaatt ctttttgtac ggttaataac tatccaataa ccgcgggagg caggccttgc
    36301 gataatatcc gggggcctgt aattaccgtt taatgaaagc ctctttatca tggcagcctt
    36361 gaattggggc ccttgtatgc cgtcggcggc aagattgcag tcgctttgaa acctgagagt
    36421 tgcatttctc aaattaatta attcatcttt ataatcatac ttataatagc ccattgattt
    36481 taatgcttct atataattat cactgttcaa cccggaaaat gtttcttcct cgggacaggc
    36541 catgagttct ttgataagct cagtatcttc cgcataaata tcaatagaaa atgccggaaa
    36601 taacagcaat accgcagcga ttaaaccttt gcgcataaaa tacctccttg aaagcatctt
    36661 aacattatta ttattctgaa aaaaatgctt tcttatgtcc gtgtttgaat atttacatat
    36721 acttcatatt catagaataa tgcgacgggg acacggggac gtttcccctg tcccacttcc
    36781 agtgggacac gcgaaacgtc cccgtgtccc cgcgtcccca tgtcctcgcg tccccatgtc
    36841 ctcgcgtccc catgtcctca tataataagg agagctaatt gctctccttg ttttaagtat
    36901 tagaaccttg ttccggttgt tcctgccata ttcctctcag ctgtctcaat tagtcttcta
    36961 accatctgac cgccaatttt ccctgcatct tttgctgcaa tatctccgtt gtaaccttcc
    37021 tttaaagtaa cgccaacctc tgttgctatt tcatatttaa atctgtcaag agctgctgct
    37081 gcatgaggta ccaccttctg attatttctt gccatccgta ttcacctcct tgagcaatat
    37141 gtataatgag ttaataatga tttacgcatt attattttgg ttaagcttca atattttaat
    37201 ccaagtaaat tttgcctgat ttggctcatt aagtgattca ttaatctacc tgaaaatcca
    37261 tcacaaaata atcattaata atgtaaataa taataatgtc attattaatg acaatacaaa
    37321 acgaagggag atcaggaaat gaaaggtgta aaaccttcag taaaagcact ttgcagtgcc
    37381 ttggcagtct gtattatctt ttctgcaggc attgcagcct ttgccgacaa caatgctcca
    37441 tcagcagtca aaagcaaaca ctacctggaa gaggctgttg aaaaaatggt gcaggaagga
    37501 agcttaacca gggaaaaggc ggataaaata cttgaataca aacaaaaaag aaatgagaaa
    37561 ctaaagaagc tgcctataga acaaaaaacc caaatgaaaa aacaaggcgg aagagggagt
    37621 cttctgaagg cactggttga ggaaggcata ataacggaag aggaagcaaa atccatcagg
    37681 gaaaagctgc gggaaatgaa ggaagcaaga gttgctgacg gattgcaggg acttatagat
    37741 aaaggagtgc ttacgaaaga tgacatcaat aacatcagaa gctatttgct gaaagtcagg
    37801 gaagagcgca agtcatccgt cgaaaagctg aaatccatga ctcccgaaga aagaaaagca
    37861 tactttaaag agcataaaaa tgaacgaaaa gacatcatta ccaagatggt ggaagacaag
    37921 gtaataacaa aagaacaggc ggaagaaatc agaaaggcaa taccggaatt aagcaagttc
    37981 agatactagg ccatgacaca gtataggaga ttcagggcag ggattttaag ttaggataat
    38041 gtcaaatacc gacgtggggg aagatatctt caaggaaacc ttcttcccct acagtcggag
    38101 tactttacaa accctctcta tgccattggt aaatcaatgc ggaatacggc tcccgcctct
    38161 gaattctccg cagttatttt tcctttatgt ttttcaatta tacacttgct tattgcaaga
    38221 cctaggccaa aattaccctt ccggccttta aagaacctgt caaaaatatt gggaagctca
    38281 ttggattcaa agcccggccc gtcatcggat atcgtgactc ttactgtttt ttcatcaata
    38341 atctctgact ttatttcaat tgagcttttt gcatagcgta tgcaattgct gagtatattg
    38401 ttgattgctc ttgaaagctt ttcctcgtct gcaattatat taatattttc ttcaggcaca
    38461 gatatgtcaa ttttgatatt gttattgcag gcaatcatat tcatacgctc tgcgcagctg
    38521 cgaagcagtt cgttatagtc cagcgtcgtg agacaatagt tttcctccat tgcatccagc
    38581 cgtgacaggt agagcaggtt ttccacaagc tgagtaaggc gtttggattc ttcaattatg
    38641 atgccggcag cttcattgct gtccgcaaca ttgtatttta tgccctccgc atagctctgg
    38701 atggacataa gcggagtcct gaactcatga gaaacattct gcaaaaaggt cttctgtgcc
    38761 ttgtcataag tctccagcct ttcggacata atatttatgt tagtcacaaa ctcgctcagc
    38821 tcatcgtaca cgggagtgct gatcttcgct ccgaaattcc tttcagcaat tgatcgtata
    38881 tgctgattca aggaactgaa cggctcagag atttttttga ctatgacgga ggagaacagt
    38941 acaataataa atgccgacaa cagaagtatt gcgaagagaa taatatttat agccatctga
    39001 agctgattta ccttctgcag gcttgagtat attattatcc accccaatcc gaaatcattc
    39061 ttctgcgcta ccggttttac tactgctata tattcggtgc cggaaagatg gaagttcaag
    39121 tagctttcct tttccagatc ctttaatttg cttatctctg aggaaatttt acctacgagg
    39181 ccctcatcgg gatccgcata caattcaaca ggtgaattaa taatgttttt attattatcc
    39241 attaaaatat aatcggcatt caataccgaa agcggttctc tcaaggatct gtcaagcatg
    39301 aagtaaaacc tgaacacgtc attggttctt attcttatcc ttggaagctc aggaggtatt
    39361 tccttgctga atatgaatat ttcagggccc cttttcaaag ccgtttcttc ggcatgggat
    39421 gctattttat tcaattgccc tattatatcc ttttcaatat agatccttat ggcagcatta
    39481 aatatgattg caaccagcac aatcaaagtg attataacgg taagattgta tttgaatatt
    39541 cgccatttta tggtattgct tttcataata tcattccttg cctgttaatc ggaagccaaa
    39601 gccccatact gtatctattt taagccttga gcctgcatct gcaagctttt tccgtattct
    39661 cttaaccatg tcatctgtag ccctggtttc tacttcgctt tcaaagcccc agaccttgtc
    39721 aagaagctca ctgcggctta cagccctgtt tctgttagct gcaaggtaat aaagcagtga
    39781 gaattccatt cccgtaaacc cgatatcctt atcatggcat tttgccttct taatattatg
    39841 atcaataaca atatcggaga cttctataac ttctccgatt cctgtctgag ctttatccag
    39901 ttcaattctt ctgaaaatgc ttctgattct tgcaataagt tccatagggc tgaagggttt
    39961 tgtcaaataa tcgtcacatc caagggtcaa tccggtaatc ttatccggct cagtatcctt
    40021 ggctgatact ataataatcg gaacactgct tatttgccgt acctgacggc atagagagta
    40081 cccgtctatt cccggcatca ttatatcaat tacaagcatg tccgcgggtt tgacattaaa
    40141 agcgtcaagt atggattgcc cgtcgttaaa tgtttcggcc tcaaaacctt ccttaataag
    40201 aaaggatttt attatattgc atatattaac ctcatcatca gctatgtata ccagcttttt
    40261 atccataggc tcatcacctc atgattaatt ttatcatgaa aacctgtaaa attgcatttg
    40321 aaaattcctt ctgctttaaa tttgccgcag ttttgcggag atttttaaat aggaaaagcg
    40381 atagaattat acatgtaaca agtcaaaagc taagaaacag ggggtagcat caatgaattc
    40441 gaacacagtt ccagtcagaa agcatttaat aaaattatcg aggaaaaagg tactgattgc
    40501 tgctgcaata ctgcttgtgg ctgcatcagt gataggattc aactattata aatcaaaaaa
    40561 ctctaagaat ccggccgtac agcaggcaac agccatagca agaatggggg accttagtgt
    40621 cgttatcaca ggatcagggc caatcagttc ctctgaaaag tttacattga catccaatgt
    40681 aaacgggact ctcacaaaaa tatatttcca ggatggagat aaagtaaatg caggagattt
    40741 gatatttgaa atagatgaca aggataccca actgcagata aaacagataa aaaacagcat
    40801 tgcacaggca gagcttacac ggagcagcaa tatatcggac cttgaagccg gaacggttac
    40861 agctcctatt gacggggaga tacatgacat gcaggttaaa gaaggagata acatatcaaa
    40921 taacggtact ttatttgtca ttacggacaa atcggaatta aaattgttgg ttccgtttat
    40981 caatacatac agaaacaggc tttccatagg gcagaaagtt gcaataaatg cttttgatac
    41041 cgaaagggac gaactgcaca ggatggaggg aaccatatcc ggcatcagta ctcccggtta
    41101 cacaacaagc agcggtgctg aatcatataa tgtgagcata gtaattgaaa ataacggctc
    41161 cttgaaagaa ggaatgattg caaatgcttc cgtagacata gacggaaccg aggtaaaaag
    41221 cagcggaagc aatacgctga gttacttgaa aaacatgacc gtaagggccc cttcgggagg
    41281 aactgtaagc aagctatatg cagaaaacgg tcagaacgtt aagaagggtg atatcctggc
    41341 agagcttgaa aataaagacc tggagcttgc tatcaaaaca aatgacctga aattggagga
    41401 tttgaatata cagctgcaaa cagcggaaga gaagcttttg gatcataaga tatacgcgcc
    41461 ctttagcgga accttaacac tgaatgatat tgagcagggc aacagcataa agcaggggga
    41521 tatactggga agtattgcaa actatgacat tatggagttc agcataaacg tggatgaact
    41581 ggatattgcc aaaataaagg aaggacagaa tgtaagagtt accatagatg ctctcccgga
    41641 tacaactgac aaaccactca agggaattgt atcaaaaatt gcagtagagg gtacatcgtc
    41701 aaacggagta tcaatctatc cggtaacaat taaaattgag aaaaatgaag cactgaaggg
    41761 cagtatgagt gcaaacgggg aaataatcgt gaatgagaaa aaagatgtac tttatgtacc
    41821 gattgatgca gtggttaaaa tgaacggtaa agactatgta agggttgtac ccggggcagg
    41881 cggggcaggt aaagctgcca gaaaccggaa ttcagacgca aacggcacag aagttaaaga
    41941 agacacaaag cagaatactg aactgcaagt tgaactgaga gaggtcaaaa caggtataag
    42001 caatgccgaa tacatagaaa tagtcagcgg gctgaaagag agagaagcag taatcgtaac
    42061 ttcaagaagc tccggcagca ataatcagag agggccgaat atgatgatta tgggtactcc
    42121 tcctgcagga ggcggcaatg tcagaatcag aaacaattag gaggagtgcg gtcatgatag
    42181 aaatatgcaa tatgagtaaa atttatcgga tcggaaatgt cgcggtaaag gcattggata
    42241 atataagcct gagcattaag cagcatgaat ttgtttcgat agtagggcct tccgggtcag
    42301 gaaaatccac tctgatgaat atgatcggct gccttgatgt acctacctcc ggagaatact
    42361 gcctggacgg catggaagta agcggcctga ccgacgacca gcttgcatat atacggaatc
    42421 ataaaatagg ctttgttttt caaggcttta atctgattca aaaacttaat gccttggaaa
    42481 acgttgagct tcccctgata tatcaaggca tacccacaat ggaacggtat gaaagatcgg
    42541 tggaagcact gaagttggtt ggcttggagg accggatcca tcacaggccg gcagagcttt
    42601 ccggcgggca gcagcagcgt gttgcaattg caagagcact ggttggcaat ccccagatta
    42661 tcttggcaga cgagccgacg ggaaacctgg attcaaaatc aggcaaggac ataattaatt
    42721 tgcttaagga gctgcatctg aaaggaagca ctatagtctt gataacccat gacaatgata
    42781 ttgccatgat ggctgagaga atagtccgga ttcaggacgg tcaaataaac gatgacagga
    42841 tggtgagcta ggaatgagtt ttattcaggc ctgcaaaatg gctgtaaaga gcatcaaaag
    42901 caacaaagtt cgctccttcc tcaccatgct gggagtaata atcggcgtat cctctgttat
    42961 tgctgcagta ggctttgccc agggcagcac caaaagtata acggatatga tatcaagtat
    43021 gggcaccaat ctgatacaag ttaatataat gggaagaaac agcaacagaa acgttacata
    43081 cagccagata aaggaatttg ccgaagaaaa cagcggggaa attacggcag tggcacccca
    43141 gtcaaacact tcggctacag taaaatataa aacaaacagc aggagcacca acgttcttgg
    43201 aacaagcccc gactaccaga taataagaag tgtttatgcg cagcagggcc ggttcctgat
    43261 tcagaatgac cttgagaata tgcagaaagt tgctgtatta ggaactgcag ttgtcaatga
    43321 catttttgaa ggaacaagcc ctcttgggga aaaaataaaa ataaacgggc agctctttac
    43381 tgttgttggc atactggaag agaaggacgg aggacaggac cagggagcag atgacattgt
    43441 aataattcct gccaccgttg cgcagaggct gttcaggtca gggataatca ggaatttttc
    43501 catacaggca actagcccgg acacagtgga aagcgcaatg gaaaaacttg aggaattcct
    43561 tgcaaaaacc tataaggatt ccgatgcttt cagggtcttt aaccaggcag agatgctgga
    43621 gaaccttgac agcatgaccg gaaccatgac agcggtactg gcggggattg cagctatctc
    43681 acttattgtc ggcggcatag gaataatgaa tatcatgctt gtttctgtca ccgagaggac
    43741 aaaggagata ggcataagga aggccatagg agcaagaaag aaaaacatac ttgttcaatt
    43801 tctgattgaa gcaataattc ttaccggatt cggcggagtc ctgggtgtgg ctgtcggcct
    43861 gtccgtaata cattttatta tcggaggatt cggccttgta ccggaagtat attcgatacc
    43921 ctggatattg atttccttcg ggatttccct gggggtaggc gttaccttcg gattgtttcc
    43981 ggcatataaa gcagccagcc tcaatcctat agaagcttta aggtctgaat aacgggagga
    44041 gaaagatgag aagaacattt tttatattat ctgccatact ttgcgtatca ttattattta
    44101 actgcattgg tgcattcgcc gatgcaacgg aggacgggct gttaaccctg gaggaagcca
    44161 aagcaatggc actcaagaat gacgtgcatt atcaccatca ggatgaaaat attcaggctg
    44221 cattagagga ttatgaaaaa ctaagcgaag atacatcgat aaactccgga agaggaagca
    44281 acattgctca aaaagaagca gcacgcattt cgaagaaaat ccagcttgaa aatgcatact
    44341 ccagcgtccg caaagtagtt ttgacaaagg cagatttgaa aagagcatca tactccgata
    44401 cggcaaatgc attctatgct gttatagaag cgcagaattc ccttgcaagc gcattgacag
    44461 atttagaatt gaagagaaag gagctggaaa aggctgaaat caaatatatg ctgaatattg
    44521 tccctaagaa aacccttacg caagcagagg aggcatgcaa aaactcagaa gctgcgtata
    44581 acaaggcagc ccttgagctt gaaaactgta tatcaaagct ttgcaaaatc ataggccgga
    44641 aattggatat gtccaaggtc aagctggata ccacacttga tatccccgat ataaaaagca
    44701 ttgatcttga taaaattaag gaagacaatc tgaaaaacaa tcttagctat ttttctgccc
    44761 gggaacagta taagctggct gagtaccaac tggcactgac tgaagaaaaa tatgaagatt
    44821 actacgatga cctgaaaaag ggttcctcaa atgtcaggga agagtttgac aacatgctct
    44881 atgatgcgca aaaaaagttc gaagatgcgg agtacagctg gaatgagaag ctggaggacc
    44941 tggaggagtc cctcagggaa cagtatgaga gccttgagga cctatatgaa aactatttgg
    45001 agcaaaagga agacattgat gatatgaagc ttaccgtaaa ggaaaacaaa acaaagcatg
    45061 ctatgagcat tattaccaaa gctgctctgg acagcagcat tgccagcctt gaaaggctgg
    45121 aaaggcaatt ggtttcaaca attataaagc taaacaagca gtatattagt ttagtacagt
    45181 gtagtatcga ggaataagct gaatttacag ccctgacggt tcatcaaatg tcccgtcatt
    45241 aagtactatc attctcattt gataaagctt atcaccatta aataattgaa aatactatat
    45301 actttatttt catttgcaat tgacaaaagt taatatatgt aatattatta tagaagaaaa
    45361 gtatataccg gaatagaaca ggcggattga atacaattta gccgggctaa aatttagcag
    45421 caggttaata tacttgtggg actataaaac agtcttgcaa gtattttttt tataggactg
    45481 ctgggtattg attcatttaa gatgtattgg gggacgaaat tattgaataa tctggtaagc
    45541 aaaatcaccg atctgtttat tataaaattt attaaaaaca ccaagccgga aaaaaaaata
    45601 tcagaccctg caactaggcg caaatatgct tatttggagg catatgtaag tattgtcggc
    45661 aatctgctgc tgtccgccgt aaaggttgca ctgggattat tccttaacag tatttccctt
    45721 ttggccgatg cagcccatac tgcggcagat gtattgacct caatagtcgt acttttcgga
    45781 ttcaagctgt caagctcacc tgcagatgag aagcatcctt ttgggcacgg cagaatagaa
    45841 ctgatcgcaa ccttggtgat ttctgcaatg ctttttttag taggctttga atttgcgaaa
    45901 tcttcttatg aacgcctttt atcaaacact ccggtcaaag gcagttatat tatagcatta
    45961 ataatgatat ttggagccat attcaaagaa tggatggcca gattctccat agaccttggc
    46021 agcagaacct cctcatcaac ccttattgcg gatgcatggc accacaggac cgacggaata
    46081 gcctcaatac tggtggctgc tgcaataata gcttcgaaat tcggttatta taaagtggat
    46141 gccgttttcg ggcttatggt atccgcgctg ataatctata cagggtatag tatttcaaaa
    46201 gattccattt cacaaatcat tggggaaaca ccggcggaag aggaactcaa taatattgaa
    46261 aatattgcct tgactgtatc gggagttcac agtattcata aggtgaatgt ccacagttat
    46321 ggctgccata aggaaatatc cctgcatgta caggtggata atgatatgtc cctggttatg
    46381 gctcatgata tttccgagca ggtggaaaga ataatagaag aaaatatgaa ctgcaaggca
    46441 atcgtccata tagaacccct tgagtaggtt tattagcacg gaggaggcaa tagcccggac
    46501 cggacccttg gctgcaaatc cttatcagaa gaatatgtcc atataatatg gaaatattca
    46561 agtaattttt tgagacaata tatcgctaat gataatcgtt ctcatttaaa ataattgagt
    46621 ctgaagcagc agccataaaa taaccaaaaa acagacaata atatttttta gtaatttatg
    46681 caggcgcgat acattttata tttttaagaa ttaaatgcat ttagcacatt tttctgagat
    46741 tataatttac ttagtttaaa tttttcttgg ataataattc acagcgatta aaatttaccg
    46801 aaatatgatt tgttgaagaa gcttgacacg taattcaata tgttttctaa tacttttttc
    46861 ttgaagcacc caggagtatt gatattgctg atgtttcaaa aaaccctcaa aaaaacgacg
    46921 cgtttaaatc gcttcagagc gatttttttt gatagggtaa tataattaga cctcgtgatt
    46981 tttattagca gaatacgcag ccggtatccg ggcagcattt tgcctggtcc ataagggcaa
    47041 ttataaattt cccggctaaa ggtgattttg ggggtcaatc aagaaaggga aattataagt
    47101 aatcacgaaa tagtaaatta atgagcattt agtattacag ataatcaaaa actcgtgcag
    47161 aaaacgccag tttaaacaat ttataaaact ttataaataa tttatcaata aaagaggatt
    47221 ttacctcttc tgtgtcgaat actttaattg cgctaaataa tcatttcgcg caatattgaa
    47281 taaataatct aaaaagggga tatttaatat gactaagctt cctgatattg cagttgaatt
    47341 tcttgaatac atggaaacca tccgcggtaa atcaaaaaat actatcaccg cttatagtta
    47401 tgaccttgct ctctttttta aatatataaa aatgaaacgc ggttatttag taaccgatat
    47461 ggaaattgat gaaattccca tcgatgatgt agatatgtat atgatcaaca atataagcct
    47521 tgctgactta tattccttcc tctccttcgc ggctaaccag agagacaata aaaattacgc
    47581 cagggccaga aaggttgcat gtataagatc ctttttcaaa taccttcata aaatgaaaat
    47641 gatcaaagaa aacccaagcg tagaactgga atcaccgaaa ataaattcca ggcatcctat
    47701 ttacctcact cttgaggaaa gcaagaagct tctttcgtcc atagacggcg agaacaaaga
    47761 aagggattac gctataataa ctctcttctt aaactgcgga ctgcgccttt cagaactaat
    47821 aagcatagat ataaacaaaa tcagcggtga catactgacc gtaatcggaa aaggcagcaa
    47881 agaacggacg gtatatctga acaaggcatg catagatgcc ataaataact acttaaaggt
    47941 ccgccctgtt gatgatgtta aagataagaa tgcattgttc ttaagtgaaa gaaagcgcag
    48001 aatcagcaag agaaccgtac aataccttgt gaaaaaatat atcggtattt ccaaccttga
    48061 tatggaaaaa tactcccctc ataagttaag gcatactgct gccactctaa tgtacaagta
    48121 cggaaaagtc gatatcaggt ctcttcagca gattcttggg catgaaaaca tttcaacaac
    48181 acagatttat acgcatttag atgatgaaac tctcaggaaa gcaataaatg ccaatcccct
    48241 ttccgaggag aaaaaataaa tcttcgaaat gattaacata aatattttta tgttaatcat
    48301 ttccttcttt ttcactgtaa tccactatat agtctctctc atatttccat tttccatgat
    48361 cttccatatc caactctgca tcttcctctt ctattatctc gtctatctca atgctgttgt
    48421 aatcagcagc cttatctatg caagtgcagc aattatcgca aatcttattt ggatcaatgt
    48481 cacatctgtc gcattctccg cagtcggtac attctctgtc cgttaaaaca cactctttct
    48541 tgtattccat atatcttcct ccccaattta tacgtcagct ctattttaca atgtattggc
    48601 actaaaatgc aatccggtca ttaacttggg aaattcttct ataatctatt cagagaagaa
    48661 tatattatgc cgatattata tttaaatgct tgctttatat ttgtataaga aggcggtgct
    48721 aatattgatt aatgcttctg ctaatatttt tgtttttaca gcttatgttt ccttgctgat
    48781 aattgagttt gtttttttga aaatactata tattgacttc aacactccct ggcttattac
    48841 cacattaata tatgttttgt tcataatata tcagatactg gtcagagtta atgaacaagt
    48901 ggaaaaaggc agagtaaagg aaattctgta ttgtatcatt gactctatta atgggatacc
    48961 gctggtcaat aaggataata aaataattat acaatgggat gtaattttta aaaaactgat
    49021 tgataaaaaa gaatactttc tatatgacaa tattataaag ataaaaacaa aaaacattga
    49081 ccgatatgtt aaccagtatc cctccctcct atttaacagc tccatggata tgctggaaag
    49141 aaaaaacaag attgacacaa aatctattat taaaggaaaa gaatccgccc cgaaagagtt
    49201 atcggaagaa ctggatttaa atgaagagat gaaaaaagtg ttgtatgtca aatatttgag
    49261 cgctaataaa atgacactgg ctaatcttga ttcttccgtt tactttgatg ttgtgttaaa
    49321 tgatgaatat gagccccctg aatttgaaaa attcggtatc aaacctgatg acagatatga
    49381 tggattttca ttggttgctt atcataagga taagaagtac attctggttc caaggataat
    49441 ggtatattgc aaaagaatcg acaaggaaaa gtggctgtat cgaaatatag acagaatcat
    49501 aaaatgtgcc gaaaatatag ctgcaggtat acaggattac tcaataattg tagatgagct
    49561 gagtttaaaa gatgtaacga ccgattacta tgaggaagaa accgaagtaa taccgccttt
    49621 ctattttgag cgcttagaca ataacatcct ggagttttcc ggaataggaa aaggcaggga
    49681 agttgcaagg gcttactgtt cattggatac atctcataat agctttgaaa tcaatctgca
    49741 aaaatatgag ttgattattg atggtgtcag cgaagttgta gaagaagaaa aaacaatcgc
    49801 cataggaaat gcgtatcaac ctgttgtttc cataatggag aaaatgccga taatatccaa
    49861 taacattaaa ctggctatat taaagaaaga tatcgaagac cttgtaatta aataagtatt
    49921 ctatttgaag cctggaaaat ataaaagttc tccgggcttt atatttgcgt tctgcaggcc
    49981 attgatctcc ataacccttg atatatattt ccttatatcc atatccccct tatgcttttg
    50041 tgacatgttc cacagtgtat cccccacttc cacatattca ggaatcatcg tgacatcctt
    50101 attctccctg gcacttactg caagcgtaaa aagcgaggac attgcaaata tcaatataat
    50161 tccaacagaa actataaacc ttgatttgct tctgataaca taccttttca tataattacc
    50221 tccatagcga acatttgttc ttttctccat tatatgcgaa catatgtact gtgtcaagaa
    50281 aaaaattaaa aatttacgaa cgtatgtttg aaatttttaa cttgttatgt tataatgata
    50341 gaagaaatta atgggaatta tatattggag agtaatatga atattgatga tttaagtaaa
    50401 aagcaatttg aagtcctgaa tttcataaaa aaagaaatca acaaaaaagg atatcccccc
    50461 tccgtcaggg aaatatgtga tgctgtgaag cttaaatcca cttctaccgt tcacggccat
    50521 cttgagagac ttgagagaaa gggatatatc cgaagagacc cgacaaagcc tcgtgccatt
    50581 gaagtcctcg acagcaacag ctctattgtt acaaaaaagg atatggtaga gctgcctgta
    50641 gtcggcaagg ttacagcagg ccttcccata cttgccgaag aaaatatcga agatacattt
    50701 ccggtgccta tagattttgt cggaaacagt gaagctttta tgctgattgt aaagggtgac
    50761 agcatgataa atgccggtat atttgacggt gattttgtaa ttgtcaggaa gcagtcagtc
    50821 gccaggaacg gggatattgt agtagcattg ttaaatgatg aagctacagt caagaccttt
    50881 tacaaagaaa aggattattt caggctgcag ccggagaatc cctacctcat gccgattatt
    50941 gcaaaggagc tttcgattct gggaaaagtt ataggagttt tcagatccat aaaataaaaa
    51001 taatccgata accgcaatat acattctaat aggttatcgg atctttattt tttgttttta
    51061 tttcttcgga caggaatcca aacttcactg taagcgtaat cttttttatt tttatccatc
    51121 ttagtaaatg aaaaggacgg agtattgatt acttcgtaat ccgacgaagg tagccattct
    51181 gaatatattc ttgccattgt attctgcagc gtggaaggaa atgatccttt attcggaaat
    51241 acggcccagg tacaggcttc tacgtgcact ttttccaata gatcacttac attattttcg
    51301 gtggttaata tacctatcag gtgagttaac tctccttctt ctcttaagaa gtcagcatca
    51361 gcatcataag aagcacttac aatctcgtaa ggctccatat tttgaagaga atgcatttct
    51421 tttttttgtt cctcagttat gctttccgca agcttaaaaa tctcttggtt gatgccttca
    51481 aattgcatag ggacacgttt acttacaccg actaaattaa acgccggttt gtcctcaatt
    51541 ctaaattcca tattcattcc tcccttgaac gattaatccg gtacactcct tactttggtg
    51601 ccttctttca gtgtctcatc cggtgacaga attatttcct cttcttctgt caagcctctt
    51661 aacacctcaa cctgctcgct gctctcgatt cccttttcga tagcccttag ttttgctgtt
    51721 ccgccttcat tgacaaatac gtggtctttg cccttgtagc tgaacacagc tttctcatct
    51781 ataaggagag tgtctttttt acttttcaca ataattttta cggtcatttc atatcccggc
    51841 ctcagccctg ctgcttcatt atccaagctt atttccacct tgactctttt ctgttctata
    51901 ccaagatcag acatgatgct ttcagctttg ggatgtattt cccttacagt acctttaata
    51961 tcttgcatac caatttcttc gtcatcaatt attacttttg aacccacact aactcctgca
    52021 atatcctcaa taagcacgtt gctttcaata taatatcctt ctgagcctcc tatttcaaat
    52081 agctttgacc ctggctgcac aatactgcct tcttctgctt cggcagttat caccaagccc
    52141 tctatagggg atgtgacaat catttcttcg gatttcagct taagctgttc tattctggca
    52201 ttaatttcgg aaagctgagc ttcatattgt ttttttatgt tccccgagat acccttctca
    52261 gcaagtgcca gattgctttt tgcagtttcc agccctgctt ctgcagctgc aaagttggta
    52321 agagagttct tataagtgcc caggcttatt gctccggctt catataacac tttattgcta
    52381 tccattgtcc tttttgcttc ttcatataca gcttctgcag agcgcacctg tgcattcagg
    52441 atacgtattg tttcatcatc cgccgaggtt ttagcttctg catattttgc ttctatccca
    52501 agcttttgtg cttctaatgc ttttatctgc agctttatat cgctgttatc aattttggca
    52561 ataacttctc cggcttttac cggatcacct tctttttttg atacttgtat aactcttcct
    52621 gatgccgcag aatacacggc tgcttcctgc tcgagcatca ggattccggt ttcttcaaca
    52681 tattcttcta tatctcccct tgtgaccttt tccaactcca cagttactgc cggacttccc
    52741 atcttgattt tcaagcctgc tataatcgct attaataaaa ttacacctat ccagattgct
    52801 tttttcttca ttttcaatac cccctatgat attcgactct tcaatgcatc aataaagttc
    52861 agattataaa cctttcttaa tgtagccagc tgtgcaatta ttacaaacat aattgttgca
    52921 gctgctgcct gtataaaaga ctccggcttc agtatataag gaagggtcat tatatcagaa
    52981 ctgaaggatt caaccatacc cttaatcata cccgcaccca aagggattcc gactattatg
    53041 gcaatcccgc ccattacgaa attctccctg catatcatgc cgtatacatc ctttctatca
    53101 aatcccatga tgcgcaaagt tgcaaattcc attgtccttt cagatattcc gattattgtt
    53161 gaattatata tcagtgcaaa tccgagaact cccccaaaca gcatgtaaaa ccctatggca
    53221 agcagcatta gatctatgta ctcttcataa gccctgtaca tatcatccac ggaatttact
    53281 gcagagatgt tagcaacatc cttcagttca tccttcagat cagcctgtgc tgctacagat
    53341 actcctgtaa tcagctgttc ctccagaagc agttcttcca ttgactcaat attcatatat
    53401 gcatttgcgc ccagatactg cttgaagatg cccgttactt tggtttcaat atcatccttt
    53461 cccggtataa agctttttat ggttattgta tctccctttt ttgcattcag cttctccgct
    53521 atggcttcag taataaatat tccgctttcc ggaagtatta ttacattgtc cttcatatct
    53581 ataaacttat ggaaggctgt ctgccggggt actcctatga cgctgactgt catttttctc
    53641 cagtcctttg ccaattcaaa ggggtattct atccgcggct ctattctcct tacttcgatc
    53701 aagttgttta aatcaactat tattctttca ttttgaggac cgttgaactg aattgtgtaa
    53761 tccatttttt gatattctcc gtattgcagc tcaaacatgc tcatcattgc atccttcatg
    53821 tataacggaa cagtattaat accataagcc attgccagcc ccagtacaag gaacgcaaac
    53881 ctcttcttgt ttctcaatat gttcctgata accatcttcc aactgaaggg cacatgcttc
    53941 caaaagaaag taaccttttc tataaaggta cgctttcccg attttggtgc ttccggccgc
    54001 atagagtcag caggcattat gcgtataacc cttcttgccc ccagcaatcc cgatacagtg
    54061 caaaacatac atgtcagcag gatcccgtaa aaaatatatc catattgtat ttttactcct
    54121 gcaagaggaa tattgaagaa aacagtataa tagcggctta tggggcctgc cagcatcatc
    54181 ccgccccata tccccaatat agcccctgcc aggcctattg ctaaagcgta ttttatgtaa
    54241 tgggaaagaa ctattctgtt cccgtatcct aaggctttca atactcctat agccattctg
    54301 tcattattga caattctgga tagcattatt gaaattatta ttgctgcaac cgtaaggaac
    54361 ataacaggca gcacggaaga caacatttct attccgtcca gctcctgttc caatacatca
    54421 ttgctcaact ggtcttccag ttttattatt ttttttacac catatttgtc cagcttttgt
    54481 tctatgcggt caattacatc atcaatatcg gcatcggttt ctgtagtaac cagcacctca
    54541 ttatagcttc ccctgtaacc aaatacggat tgtgcaaaag cttcactggt gtaagctact
    54601 ccgaactttt caggagccgg cataagagat tgttcgtttt ccataagata tacgaattcc
    54661 gagcttgatg caattcctga aacctttagg ctgtgtattc ttccgttgat acgcggataa
    54721 ataacatctc caattttgat attccttgcc ttcgcaaact gctccagcag gattacactg
    54781 ctgttgtctt ccagtttcat actaaccttt tgttctgagt aaagcttgtt aatgctgcct
    54841 ccgttatcag gaattgatat aagtcgaatt ttaacccttt ctttttcttc ttccacttcc
    54901 aaggctacat caacacatac acggccttgt acatcacgga ctccttcaat tgactgcaaa
    54961 ttatcaaggg cgccctgagg aatttttacc agttgaattg tcagatcact gatatttgtt
    55021 attttgtagt agtagtcaac tgaattcctt agatttgtgc ttgttatgct gaacaatata
    55081 tatatgcata acgcaacagc aacaattacg gtaactgaaa cgaactgccc ctttgaatgc
    55141 cttaccattc tcagcagtct gacatccagc tttctcatca ccattcaatc ctttccggag
    55201 gaatcggaga agcattttcc tttatttcta ctatgcgtcc gcttctcatc tttattatac
    55261 ggtctgccat atctccaatg gccatgttat gagtgataat aacaacagtt ttttttaatt
    55321 gccggtttat tttatgaagc aatgacaata tcaataggcc ggttttataa tccaatgcgc
    55381 cggtaggttc atcacaaagc agcataagcg ggttttttgc aacagctctg gcgatagcaa
    55441 cacgctgctg ctctcctccg cttagttgtg aaggaaaatg atctttcctg tctccaagtt
    55501 ctaccgattc cataacctcg tcaatattca aggcctcctt gcacatttct gttgccagct
    55561 ccacattttc ctttgctgtc aggttagcca taagattata gaattggaat acaaatccta
    55621 ccttatccct tctataggct gtcagcttcc tgtcattata tttattaatt tcatcgcctc
    55681 tcagaaatac tttgccctgg gttggcaagt ccatccctcc gatgatattc agaagagtac
    55741 ttttacctga accgctgggc ccaagtatag caatgaattc tccttcgtaa atctcaaagc
    55801 ttgcatcaca aagcgcatta acggtaacct ctcccatctt atagtccttg cttgaatttt
    55861 ctattttcat caatatcttc ttctgcactc ttcaccccct cctttccccc aaacagcccg
    55921 aatttcaaaa aatcaataaa attgccgtaa aacttaatca ttttgccttc atcgctcgga
    55981 aagtcaatat tcaaaagtgc cttcggcaaa ttcatcaaca gctcggatat aaaacccata
    56041 tccagttccc gaatctcttc gttcctaatc ccttcagaaa taatgaactc aaatatggga
    56101 agcatgaaat tcttcttgat ttccgtcact ttatccacaa tgcttttctt ctccatgata
    56161 tcccttatga gtacgagtga ataattcttc gccatatttg ttgaatagtt ataaagtatt
    56221 tcaattcttt caattgaact gccgctctca cttattgcct tcattatttc atttgcctga
    56281 tactcaatat ctttcctgat tacttccatg aacaaatctt cttttgagtt aaaatactta
    56341 tatatagtca ttttgcttat tccggcctga tttgcaattc tgtcaacggt gatagcgttg
    56401 tatccgtatt tcagaaatag ttctttcgac ttatccagta aattatttaa ctttttttca
    56461 gtttgcctgt tcatttccat tatcactccc aaattttcag acaagtatac ttttatactg
    56521 attcagtata atattatagt acttcattcc gtgtactttt tcaattgtct tgaataaaaa
    56581 accgttgtaa agagaacaaa aaaagtatca ccaactactt aaaacagttt tgcgataccc
    56641 tttttgattc ctatagttat atatctatca aattcttctt atagtcatta tacagctctt
    56701 ttaattcgct cattttttca gccatttcct tttgaagctg tgacagggtt tcatattcct
    56761 tttcttttgc agcttctcta actctcgtga agagtgattt tgaattatag gtgtccttca
    56821 tcagatagtg cctcaggtga tcaacctgcg cccaataagt tatatccaga tcctgggctt
    56881 tctgtttgct gtgaacagcc ctgcactttc tcacagtatc catgttttca ggaagcagcc
    56941 tgtttattat ctcagttacc catctgttca tgacggctgt ggtaaagctc tttaccagcc
    57001 tttcatccag aacatttccc tccagtatca cactgagctt ttcaggatat tccgctaaat
    57061 ttttaatatt ctcatatact gttgccggat gtactccaaa taatctgttt ctttcctcct
    57121 gggaataata ctcgaaaaca tcctcctcgc ttctgtatgc cctgcctttt tcaaggtaga
    57181 agccttcgga ctccggcgcc tttgatattt ccttttcaag ctcggagttg gttttgcctg
    57241 attcgcaggc agcctttatg ccatccagca ttgactgata gattgaagcc agcgcaagat
    57301 atgtgtttgt cttaggattt ggtgatctga cctcaaaacg tgtggcaagc ggattactca
    57361 tatttcttac caatcctgca agtacagttc tgttcctgga tggtgtttca acattatggc
    57421 ccaaggagca gactatgcaa accggtgctt caaatcccgg cttaagcctg ttaaaggcat
    57481 cattggtaaa tgaaatgaaa ggatttatag cttcatagtt cttcagcagc cccattaagg
    57541 ctccgtatcc gataatactt aaataatcct ttttcatatc cgacggtgta aacagattca
    57601 ctgttttgcc gttcttaagc cttgcagcaa ctccaatatg agtatgttcg ccgtttcctg
    57661 caaccccttc aataggtttt gcgttgaatg taacatcaag cccgtttttc ctgaatactt
    57721 cttttacaat gtatctgacc catttttcat tatctgctgc ctggagggca gtatcatatc
    57781 tccagtctat ctccagttgt tccattatat gagtgaggtt gcctgtttca tcaagcttcg
    57841 cctttatgcc tccaacctcc ttatgcccca tttcaggctt cagattgtac ttatccatca
    57901 atataagcga ctgctccaga gaggttctga ctattccctt tgttcttttc caatatgatt
    57961 cctgaagttc ctgggaagct gtaagggctt ccacatctgc cttgtcgttg ggggtcatta
    58021 cccagaattc aagctctgta gctgaagtca acagtatttc atcaatatcg ccgcagctaa
    58081 ttccgtattc tttgcataca gagggatgtt tttcaagaat cttcattatt tcagacctga
    58141 aacgatttat tccacgtatc aggattgatc ttgagtctac atacttattg ccgtgcatca
    58201 gaaaggaagg aattctcaaa gtgccaaccg gcctgcctgt ctcataatca atgttctcat
    58261 aattataatc tacgaaccac agggaattgg tatcggcaac caaatccaat ttcccgtcat
    58321 taagtgttgc tatgccgggc agcacaacac ttgatccgtc agtttgaaca gaaccgttaa
    58381 gaaactcttc tatatcctcg ataaaagctc ttacaggtat tctttcatct gtgctgttgc
    58441 ctgtcagatc tgcgccggcc agagatacaa agcgtatttc cgggtgatcc ttcagcaggt
    58501 ttataagttc ttccctttgg tactcctttg gattaatcac atataatata tcattcttaa
    58561 tcaaatcact tagacttgtc attactgcca cctcgtttca aatatttctt caataataat
    58621 taaaaattat accaccagga aagttcaatg taaataaaac aaaagcgcag tactaataat
    58681 actttctaat atagtcatta atactgctta actaatacct tgcaaagcct gattgccgca
    58741 tgcagcttta tatacttatg caccctttgc ctgccaggtt ctgcagagca taaataagtc
    58801 cgatttttac atgttcaaag gtcactcccc cttgcaaata tgcaatatag ggaggcctca
    58861 tgggtgcatc cgcgctcagc tcgatagacg ctccctgtac aaagcctcct gcggccatta
    58921 tcacctgatc attgtagccc ggcatatccc aaggctcagg atctacaaag gaatcaaccg
    58981 gtgaaccctt ttggatacct tggcagaaag caattagcgc atctctgtta ttgaacttga
    59041 tagcttgaat gatatcactt cttttagtat tgtaatcggg attgacttca aaacccagct
    59101 tcgaaaacag ccttgaacag aatactgctc ctttgactgc ttcagatact atatgaggag
    59161 cgataaaaag tccttggaaa aaaagcctgt taatcccaag ggtagccccg cattcttttc
    59221 ctatgcccgg agcgacaagc ctgcttgcag ccatttcaac caaatcagcc cttccgcata
    59281 catatcctcc ggtaggtgct atacttcccc cgggattttt aatcagtgag ccagctataa
    59341 tatccgcacc tgcctctgta ggctccttga aatcgaggaa ttctccgtag caattgtcaa
    59401 ccaaacagat tatattcgga ttcattatct tgatgaattt tatcgtatat tcgatatctt
    59461 ccatagtcag ggagggcctc caggaataac ccagggatct ttgtatcagt atcatctttg
    59521 tcttttcgct tatcttcttt gttatttcat tataatttat ttttcctgcc tcagtaagct
    59581 ctatctgttt gtacaccact cccagctcgg tgaggcatcc ggcagggcgt ttatcttttg
    59641 ttccgataat gttctccaaa gtatcataag gctttcccgt aaccgagatg agttcgtcac
    59701 cgggccttaa tacaccaaaa agacaaagag ccagtgcatg ggtgcccgat actatttgcg
    59761 gccttacaag ggcatcctca caagcgaata cttccgcgta taccttctcc agggcttctc
    59821 gccccacatc cccgtagccg taaccggtag aacagttaaa gtgcatttcg cttatcctgt
    59881 tcctctgcat agcggcaagt accttgtact gattaaactc acttacctca tcaatctttt
    59941 tgaattgtac cgagacttct tcctcggtgg atacgcagat atctattatc ttcttgctta
    60001 tgttcagctt gttaataaga taatctgttg ttttataatg cataatatta cctcttttta
    60061 atataaattt tcaatctgtg tattttatta agcatctaac tgtatattac ttcagttaaa
    60121 aaaatttgta tgaatcgcaa ttattttatc atatttcaca gaacaagaca acgcacctta
    60181 aaattcggat actataaatt tagtattcca caaaattcga aaaatgcgac ggggacacgg
    60241 ggacgtttct cccggggaca tggggacgtt tcccctgtcc cgctttcagt gggacacgcg
    60301 aaacgtcccc gtgtccccgc gtccccgtgt ccccgccgta ttattcttct aatatgaaag
    60361 ggatatcgaa aactatttat tcaatagttt tgcgataccc ccttgattgt attgggatca
    60421 attatttaac tgtattttcc gtcggtttgt tatcgctgaa agcgaaatta atcggtttta
    60481 caggtataat agttgaaatt gcatgcttat aaaccagctg ctgcttccct tcgttatcca
    60541 gtactattgt atagttgtcg aagcctctga catttcctct gagctgaaag ccatttacca
    60601 gatagattgt tatagtgatg tgctctttcc tgacctgatt caaaaacaaa tcctgaagat
    60661 ttatatttac cttgttcatc ctattcccct ccaaaataaa ataagcaaat accaagtata
    60721 tgtatattta tattattata taattattag ttatttattc tatattaaag gaatttttcc
    60781 tgcaataaac ctaattatat tttccactat gacttccttt ctgaagaaat tgtcaacatc
    60841 cacccaattc acccttttat cgcctctgaa ccaggtcagc tgcctttttg cataatgtct
    60901 tgtatctctt ttcattatcc tcactgcttc gtctttcgta atcaatccat gcaaaaaatc
    60961 cgcaatctcc ttgtagccca atgcctgcat tgaattaagc tctctgccgt atcctctctt
    61021 aataagccct tccacttctt ctaccagtcc ggatgccatc attctgtcca ccctctgatc
    61081 gattctttca taaagctttc gcctgtccat tgtcaatgtg atgtatgcaa agtcatatct
    61141 gggcggttgt ttcctggatt tttcctgaaa gtatgaaatc ggcctgcccg tatgtttata
    61201 tacttccagg gctcttatca ctcttctcag gtcattcgga tgaagcttct tataagatgc
    61261 cggatcaaca gcttcaagca ttgcatgcac ataatcagtt ccttttactt gagctgtttc
    61321 ctgcatttcc ctcctgtact cttcatctga aatggagtct gtaaaatcaa gattattaag
    61381 cagggagttt atgtaaagcc cggttcctcc cgttattatc ggcagcttgc tcctatcatt
    61441 gatatctttt atatacaatt cagctttttc tctgaattgg gctacgctga aatccatatc
    61501 aggagtaact tcatctatca tataatgctt aattccctgc atttcttctt ccttgagctt
    61561 tgccgttcct atatccatat atttataaac ctgagcagag tccgctgata ttatttcacc
    61621 gcaaaggctt cttgccagag caagggatat ctcggttttc cctacggccg taggacctgc
    61681 gagtatgcaa atttttggca gcaatttaaa cacctctgaa gctatattat tcttttgaac
    61741 tttttctcca tctcgtatct gctcattgtt attatagtcg gccttccgtg agggcaggta
    61801 tatggctggc atgttttcat cagcctctcc agcagcacac ttatttcctc cggagtaatg
    61861 gtgtcccctg cctttatcga attcttgcat gccatgctta ttataatctt ttccaaaggg
    61921 gaggatgcat taggtgccga ttccttgaga gaatcaagga tattgctgaa aaattctccg
    61981 gtacaaggct cacccattaa tatcggcact gctcttattg caagagtgtt ttctccgaac
    62041 cattcaattt caaaaccaag attctgaaaa ataaggctat tttctgcata cagcactttc
    62101 tctgcaggac ttaattcaat aactataggc atcataagct cctgcacgga aatattcttt
    62161 tcattatact tatcttttat atactcgtac attatccttt catgggctgc atgctggtct
    62221 ataatgtaaa aatcatcatc cttctgcccc agaatatatg tgccgaataa ctgtcctatt
    62281 actcttattt cattgtcgaa ctgtttttgc tgtaccttcg tcttcctgtc ataatcgtac
    62341 tgccacttat agctgtcggc ccgcggagct gcagcctccc ttccgaaaga aagctggtca
    62401 aaagacactt tttccggctc gtaggagttt ctgctttcaa atgcagctga cggcgccgtc
    62461 tgaggttccg ttacatcctc aaggttctca aataccagtc tgttgttatt cagacagctt
    62521 tttaccgcca ggtacagtgt attgaaaata gccctttcat ctgagaactt aacctcctgc
    62581 tttgtcggat gcacattaac atccgtcatt tctgatttta tattgatgtt aataatgctg
    62641 aaaggatagc ggttattcat cagaagggtt ttatatgcct gcttcacagc ttctgacatc
    62701 attctgtttt ttacatatct tttatttaca gaaaaggtca tataggtaga attgcctctt
    62761 gcaatttcag gctttcctat atatccttca atgcttataa tatcatttga atattctaga
    62821 gggagcatcg ctcctgcaac gctcttcccg tatatgcaca gtataaccga aagcaaatca
    62881 ccgtttccgg gggtatgcag aaccactttg ttgtttactg taagcttcat ggatattccg
    62941 gtatttgtca tggcaagctt ttcaataatg tcaactatgg ctccctgttc cgatgtatcg
    63001 cttttcagga acttcagcct tgccggggta ttataaaaca ggttccttac agtaacgctt
    63061 gtgcctgttg gatagccgat gcttttactc tgcacaagct tcccgccctt gataatgaca
    63121 tggcttccgg ctgcatcctc ctccgccttt gttaaaagct ccacctcaga tactgcagct
    63181 atgcttgcca tagcctctcc gcggaagccc agggtgctaa ttccctgaat atcggcggag
    63241 tcttttatct tgctggttgc atgcctgaaa aatacttgct cggtatcctc cttcattatt
    63301 ccgcagccgt tgtccgagac atttataaag ctttttccgc catcctgtat ctcgatgctg
    63361 acagctgccg cccctgcatc tatagaattt tccaccagtt cctttactat agaggcgggc
    63421 ctttctatca cttctcctgc cgcaatctta tttattgttg cctcatccag aatatgaatt
    63481 ttccccataa tattttctcc tatatcatat ctttggcctt atcctgcagc tcctgcagct
    63541 tgttaagcgc ctgaacaggt gtcatgttaa ccagatccac tgatttcagc tcttcagcca
    63601 atgaaaaact agcaaaatta aataggttag gctcatggtc gtttataacc ggattccttc
    63661 tccttcgcct cacatataca tcagtgtgac tgttcttttc ggatttctga agtatcgata
    63721 ttatctccga ggacctttca agtaccgctt ccggtattcc tgcaagccga gccacttgaa
    63781 ttccgtaact gcctcctgct ccgccccttg cgattttcct cagaaatact atatcttttc
    63841 ccttttcctc caccaggatg caatagttct tgaccccttc aaccttgtcc tccagttcgg
    63901 taagctcatg gtagtgggtt gcaaaaagag tttttgctcc tagccttgct ttgtcagcta
    63961 tatattccac caccgaccag gcaatgctta gcccgtcgaa ggtacttgtg cctcttccta
    64021 tttcatcaag tattatcaag ctcttagagg ttgcattatt tatgatattg gcaagctcgc
    64081 tcatctccac catgaaggtg ctctggcccg atgccaggtc gtcggaggcc cctactctgg
    64141 tgaatatcct gtccacaact ccaatctccg cttttgatgc cggaacgaag catcctatct
    64201 gagccatcag cgttataagt gccacctgcc tcatataagt ggatttccct gccatattgg
    64261 gcccggtgat aataagcatc ctgttctcat cattatccaa ctgtgtatcg ttgggtacga
    64321 actttagttc gttgtcctgg gcttccacca ccggatgcct tccgtttttt ataattattc
    64381 tgtcctcatt atttacttcc ggccttacat aattatattt ctgggatacc tcagccaggg
    64441 aacacaggca gtcaagatcg gaaaggcatg aagctgtctg ctgtatcctc ttgatatgcc
    64501 ccgctatttc attccttatt tgtataaagg tctggtattc cagttctata agtttttcct
    64561 ccgatcccag caccaggtct tcaagcctct taagctcagg agtgatatat ctttccgagt
    64621 ttgagagggt ctgtttcctg ataaaatact ccggaaccga aggcagattt gattttgtta
    64681 tgtcaatata atagccgaat accttgttaa agcctatttt taaagacttg atacccgttt
    64741 tttccttctc cgcctgttcc agctccgcaa tccactgctt tccgttgacc gatgcagaac
    64801 gtatctcgtc cagttccgga agataaccat ccttaatgag attcccttcc ctgagtacaa
    64861 agggcggatc ttcattaatt gatgcatcaa ttattccgaa caaatcctca agcacatcca
    64921 acttcccgta tatatctttc agaaggatgc ttgtacaatc agacagcagg ctctttatat
    64981 agggcagatt cctgattgaa tttttgaagg ctatgaggtc ctttccgttg gcacttccca
    65041 aagctatgcg ggttgccagc ctttcaacat catacatgct tttcaggtac tccttaattt
    65101 cttccctttt cagaaaatca tcatacagct cagctacagc gtcaagcctg ccgttaattt
    65161 caaccgtgct caaaagtggc ttttccagcc agcttttaag cttccttcct cccatggcgg
    65221 tggaagttct gtctattact gacagaagtg ctcctttcct ggatttgccc ctgattgtct
    65281 ccgaaagctc cagattcctt ctggtgctct ggtccagcac catgaaattg cttatgctgt
    65341 atgtcttaat atagtttata ttatccaaac tgaccttttg aatgtcatca agatataaca
    65401 gcaatgcccc ggtacttctt aatagtgttt cattgtctat tccgagccct tcaaggcttg
    65461 atacaccgaa ctgccttttg agccttcttt cacaggcttc atggtcaaaa tatctcttgt
    65521 ccaatatgtt ataagcagaa tatgcagctt tgatatcctg aaaatctgtg ttggagatta
    65581 tttcagccgg attaatcctg tttatttcgt ttctgacaac tgccgcatcg cttatatccg
    65641 cataagtgca gtaaaattct cctgtggaaa ggtctgtata ggacaatgct gcctgcctgc
    65701 cgtcggcata taagcttaac aggtaattgt tgactctttc atcaagcatt gagctttcta
    65761 ttacagtacc gggagttatt atctttatta cctccctgcg gactatgcct tttgccagtg
    65821 ccgggtcctc aacctgctca catattgcca ccttgtagtt tttctctata agccttgcaa
    65881 tatagctctc tgccgcatgg tacggaatgc cgcacatagg tgccctttcc tcctgtccgc
    65941 agtcccttcc cgtaagggtt atctccaact ccttggatgc ggttattgca tcctcaaaaa
    66001 acatttcata gaaatcgccc agtctgaaga acagtatttt gtccttatgc tggtttttta
    66061 tttcaaaata ttgcttcatc attggtgtaa actgcgccat tattttttca cctcatcaac
    66121 catttgaccg ttcaaaaacc atgaatggat ttcatttatc cttacattta ccagctttcc
    66181 gattatcccg tcagttcctg tgaaattcac aaccttgccg gttcttgtcc ttccggtaaa
    66241 ttttgaaggg tcattcttgc tgctcccctc tgccagaact tcaacgattt tgtccatgta
    66301 ttcatcattc agcttttttg atatgccatt ctgaagctct accagctttt caaaattcct
    66361 gtgtttatcc tcatagccgg acgtatcctc catttcggca gcagaagtac cgatcctcct
    66421 tgaatatata aacataaata cctgatcgaa ttccaccctt ctcaatacat caagggtctc
    66481 ttcaaaatcc tcgtcgcttt caccggggaa accaactatc acgtctgtac tgagtgccac
    66541 tccgggtatt tcctgctttg ctttaaatac cttattaagg tattcctctt ttgtatactt
    66601 cctattcatt cttttaagta ttttgtcgct ccctgcctga aaaggcaggt gaagctgctc
    66661 gcataccttt ttgcagtccc tcatggtata aataagctta tcggaaatat ccttagggtg
    66721 tgaggtcatg aaccttattc tctcaatgcc ttctatttca tttaccattc taactatatc
    66781 ggcaaaatcc atttccatat tcaaattctt gccgtaggaa ttcacattct ggcccagaag
    66841 agtcacttct ttgcacccgt ctttggcgag gctttcaatt tctctgatta tgtcccccgg
    66901 cttcctgctc ctctccctcc ctcttacatg aggcactatg cagtaggaac agaaattatt
    66961 gcacccatac ataattgtaa cccatgcctt gaacttttcc tcccgcagca cgggtatatc
    67021 ctctattatc atgccctcgg tgttcattat atctatcagg gtatatttct cattaattga
    67081 acgttcaaga agctccggga gcttgtatag gttgtgagtg ccgaatatta tatccacatg
    67141 ccggtatttc tttctgatca tttcaactgc atgctcctgc tgcatcatgc agccgcatac
    67201 gcctattatc agtgacggat tgtcttcttt aagctttttt aatgcaccta tgtttccgta
    67261 cagctttaat tctgcgtttt ctcttacaca acaggtattg attattatta catctgcctc
    67321 ttcttttata tctgttttgc catatcccat ttctgcaagc atacccatta ttttttccga
    67381 atcggagttg ttctgctggc agccgaaggt tatcacatgc atgcgtttct gctttcccgt
    67441 cttaagcttt tcaatatcat tattctctct tactctcagg gctatttgct tttgccgttc
    67501 tatttcagca ttattaactt tctctggtgc ttttctttcc gacatatatt tacctcaata
    67561 tttatttaat tcgcatataa agaagaatgc gacgcatctg tagtttgcat agacgcgggg
    67621 acatggggac gtttcccctg tcccgctttc agtgggacac gcgaaacgtc cccgtgtccc
    67681 cgtgttaccc gttacctacg tcgcattatt cttctattgc gctattatat ttttgttcat
    67741 attataacat atatatacaa atttatcatg aactttaagg caaaagaggc aaatcgcaat
    67801 cttttaccct tgaggggtat gcaataacct gctcgtaagg cggaggcaaa agatccgcat
    67861 tcctgctctc agttatcgga atatcaattc cgctttggtt ttcatcactt tgtctcatca
    67921 gttcctctac aattattctt cctatttgac ggcccagcct caaaccttcg gttatatcct
    67981 caataaaatg tactcctgca taaagcctgg atatgctgtt ttcctccgcc agttctttta
    68041 atctttctgc ttcgggaggg aagaaatagc tgagtatgac ttccgcagtt ccggatatca
    68101 ctgagtgtcc ggaaggatag ctcggaaact tcggagtgca taatgcggta actaaccttt
    68161 gatccaactg attcgggcgc ggcacatccc ataaatactt gaaataccaa gtaatcacaa
    68221 aggcatcatt aatgcctgcc tgtacagcag ccagcaccct tgctgcacgt accggagaaa
    68281 atccgtaagt atccagcagc cggtcgataa tcggcgtcca ctgtttggtt gcaggtccgt
    68341 ccccccaata ttccgcgatt ttgcgctgct tcggagtcaa atgcttaagc acgcccttta
    68401 caattaacac ttacactgct ttttctttag tttcaggcag aatttccata ttaccatccc
    68461 ttcaaaattt aatgggttaa tctgccaatg ctcacaccca attgaatgat tgtccattct
    68521 caattacaga atacgtaatt ttattaaatt tgtgcaatca gacaaaaaat aaaatagcca
    68581 agtactattt ttaataagca cccggctttt ttattgattt ataatactat ccttttttct
    68641 cactatactt gtttttcctt cagagatata tttatatcaa attctcctat gggagttgta
    68701 agaattaact gtaaagccct ttcaccggcc attgtgaatg atttgtattc cccgataaaa
    68761 atctgcggag ggacaatatc gcatgcgtag ttgttttgtg ccaaaagcgt cattgcattg
    68821 ccgcttatta tattagccac ttcccctaaa gcggaggatg caaagctgtc aatcctgtcc
    68881 atttccattc cggacattat ccttatcatt tccaaagtca tatctttcgg gaatccaaag
    68941 aggatagtgc ccctcaaatc tccggtaact ccaagtacaa cacttgcatc tttgctgctt
    69001 accatatctt caatgactct aagatccttt ttctgaggat ctatccccac cattagttta
    69061 aatacttcct tagttgcctt ataaaaggag tttatatatt cagccttcat tatgcttctc
    69121 ctcccttgct tttagcatac tcccctatca tctgatccat tttccccaca tgcattatca
    69181 gccaagccat aagctttccg ccgaattcct gtatcttttc ttcgttgaag ccttcctcct
    69241 caaagattct tacataatca tttattccct ctttaaattt taaatgtgct attttatgat
    69301 tctcaatttc cgggtaaccg atctgttcct ggtaagcttc ctcatcgtca aaatggacta
    69361 ttacgtactc ctgcatgaag gccattgttt ccttaacctt ttccagcttg tcctcccagt
    69421 tgtccttgct ctcaactgtc cggagaaagt cagaaacccg cttgaaaagc tctttatgct
    69481 gctcatctat aagttctata cctatactat acttgtcttt ccacatcata gttaaaaccc
    69541 cccttttctt tgtaattatt tttctttgat tattattata tataacatat tatagcataa
    69601 tttgccatat tgaacattaa aattaagcgc aataaaaatc aagcaaagaa aacctcgctt
    69661 gattcttaat aatatttcgc attaaatcat tctttacggc tacttcttaa cccccaggta
    69721 tgcctcctgt atttttggat cgcttgctat atcacttgcc tgtccggaag ctgatatttt
    69781 cccctgttcc aatacatagg ctctgtcagc tatggaaagc gccttatatg cattctgttc
    69841 caccaggagg attgtttttc ccatctttct tatatccaat attatgtcaa aaacagtctg
    69901 caccaataac ggggcaagtc cgagagacgg ttcatcaagc agcataatct gcggactgct
    69961 catgagtcct ctgcccatag caagcatttg ctgttctcct ccgcttaggg ttcctgcaac
    70021 ctgattttcc ctttccttaa gcctcgggaa aagagaatat accttctcca ggtcctcttt
    70081 aatctcattt ttgtcatttc tgaggtatgc acccatatag agattgtcct taacagtaag
    70141 attggcaaat acaagcctgc cttccggcac ttggcaaata cccgttttga ctattttact
    70201 gggattaaat cctaatttgt tgtcgttata gcttatttcg ccttgtttat actttaagaa
    70261 ccccgatatg gcattgagca gggttgattt tcctgcgccg ttggaaccta tgatagatac
    70321 tatttctcct tcattgacct cgaggtttat cccctttaat gcatgaatcc cgccgtagta
    70381 tacatgtaaa tccttaactt ttaacatagc tatacctcca cccccaaata cgcttcaatt
    70441 accgacttgt cattctgcac atctttggga gtaccctctg caagcttgca gccgaagtta
    70501 agcaccataa tattttcaca gacacccatt atgagatcca tgtggtgttc tattatcagt
    70561 atagtcagat taaatttctt tcttatttcc gatataagct tcataagctg ttctgtctca
    70621 tcaggattca tccccgctgc cggttcatcc agcagaagca gtttgggcat aagcgcaaga
    70681 gcacgtgcta tttcgagcct tctttgcagc ccgtaaggca aattgttggc aatcaggtct
    70741 tttctgtctt cgaggcccat tattgcaata agctcagcgg cttgttcatt cagctttttt
    70801 tcctgcattc tgaacttcgg cgttctcagc accgaatcca aaagtgaata gtttgcgtta
    70861 taatggcatg ccgtaagtac attctcataa acagtcagtt ttttaaacag ccttatgttt
    70921 tgaaaggttc tggtgattcc tgattctgct atcaggtacg gctccttatt agtaatatcc
    70981 ttgtcgtcga atattatttt cccgcttgta accttgtaaa cacctgttac gaggttgaat
    71041 attgtggtct tgcctgctcc gttaggccct ataagccctg tgatgctgtc cttttcaaca
    71101 ttgaaatcca cgtccccgac tgcggtaagt cccccgaatg tcttggtaag gccgtgcaat
    71161 tcgagtatgc ccatattact tgccacctcc tgcacgctga agcttattct cacgctttgc
    71221 cgccatattt ctaaaaagct ttttcatact ggagggggtc agctcgtagc cgcccatcaa
    71281 gccttcaggc cgcgatatca tgatgaatat aaccgccaat ccgtagaaca ccagcctcca
    71341 tgaagaaaaa gcccttaata tctccggaag cgaggtaagc actaaggcac ccagcacact
    71401 gccggatatg ctgcctaatc cgccgaatat tacaatgata gtaagctctg tcgattttgc
    71461 aaggctgaac atcttaggct gcaggaaggt catgtaatgc cccatcagcc ctcctgctat
    71521 tcctgcataa gctgcacttg taaccagaga agtcatctta taattaaaaa cattaatacc
    71581 aacaatctgt gaagccaact cctcctccct gactgcgatc atgttccgtc catgccgtga
    71641 gcgtatcaaa ttggcaagta caattactgc tacaatatta attccaagta cattccagac
    71701 atttgtataa tggggtatcc ccggccagcc tcttgcaccg ccgaaggtct gtacattatc
    71761 cagtatcagc cttattgcct ctccaaaacc caaagtagca atacaaaagt agtcaccctt
    71821 aagattcagg gtcactttac ccagcgccgc actgaccaat gcagccgcaa gcccgcctgc
    71881 caaaagtgcc ggtatgaagg gccaatgaaa ttttaccgtc atcagtgccg tagcatagcc
    71941 tcctattgcc ataaaacctg catgtccaaa ggagaacaag ccagtaaatc ctgtgagcag
    72001 cgataacccg agtaccgtca taaggtttat tccggctaat gtgagtatac ccaatatgta
    72061 ttcatttccc atatctatgc ccccttatgc tttatcctcg gtaaccttgc ccatgagtcc
    72121 cataggcctg actaccagaa cagtaattaa tagtacaaat gcaataagat ctctatactg
    72181 agatgaaagg taccctgctg taaaggtctc aataatcccc agcagcacag aacctattac
    72241 agcacccggc aggcttccca agcctccaaa taccgcagca ataaatgatt tatttgtaat
    72301 aatgcctatt tggggatata cggtatattt cataccgaac agtactccgg ctataccggc
    72361 taaaaaacca cccataagga atactgtaat taatataaag tcagtattga cccccattaa
    72421 ggcactggct ttcaagttgt aggaagtagc ctgaatagct tttcctattt ttgtatgatc
    72481 aatgatataa atcagtatta tcaggcatat ggcggatatc aggaacattg ctatatcaag
    72541 ctttcccatt gccaattcac caatttttat cggctgttta ttaaatacct caggatatgt
    72601 cctaaaggtc ggtccaatgg caggaatagc tattacaaga ttctccataa aaatagatgc
    72661 acccatcgct gatattataa aatacagaaa tggcgctctt ctttctctaa gaggcttata
    72721 tgccactctc tcaataatca gtgctaaaat ggcagagcat atggcagccg aaatgaatgc
    72781 caaaggaaaa ggaaacctta aaaatgtaag aaaaaagaat ccgaaatatg ccccgaacat
    72841 aattacaccg ccgtgggcaa aattactgaa attcataata ctatatacga gagaatagcc
    72901 tacagccatc aaagcatata cacttcctat ggaaagaccg tttataagct gttgaaagaa
    72961 ttgtgtcatt attttcttct cctttacacg tttagggaaa ataaactgta ttgagcagaa
    73021 agaacattta tagagcagaa ggagtttcct tctgctctat ttcatatggt caattaaagc
    73081 ctattctgca gcaaacttct gctggaatac atacttgcca tcaacaattt ttagtatagc
    73141 agcatcttta ccttccggat tatgggtatc cttgcttatc tttattcttc ctgtgatacc
    73201 ctttatatct gtagtttcaa gagcttgagc aatcttttcg ccattggctt ctcccgctct
    73261 ggtcaaagca tctacaaaca tgagaactgc atcgtgtaca agataaccgt taagctcaac
    73321 gtctttttcg tactttgcct tgtatctggt cttgaagtcc tgaacttcag gatcatcata
    73381 atccaaatga tttacgaaga aggaaccttc tacggcatcc ttcgccatgc tgataagctg
    73441 ctcggacggc catccgtctc cacccatgag tgttgcattt atgccaagct ctcttgcctg
    73501 attggagctt aatgcaactt ccttaaagaa gtaaggcatg aatataacct caggatttgc
    73561 tgccttaatc tttgacagct gcggcctgaa gtctacgtca cctgtcttga agccttcctt
    73621 ggcaacaatg gttccgcctt tttttacgaa gaactcttca aagaactctg taagtccctg
    73681 tgagtaatca tccccaacat cataaaggac tgcagcagtc ttggctttaa gaacatcagc
    73741 cgcataacct gcagcaacgg caccctgata ggggtcaata aagcatactc tgaagttata
    73801 tggctttatt ttaccgtcaa gtacagtaac ctttggattt gttgcaactg tagcaatatc
    73861 cggaaccttt gcctcttcaa ggacaggggc tatcggtata gcattcccgc tggcatttgg
    73921 gcccagtatt gtgcaaacct tgtcctgagt tgtaagtctt ttagcaacat ttacagcttc
    73981 ctgcgggtcg ccttttgtgt cataaccgat aacttccaat tgctttccaa gtactccgcc
    74041 ttttgcattg atttcttcaa aaagcatctt gacagtattc agttcacact gtccccatac
    74101 tgcctggtca cctgtaagag aacccatcca gcctactaat attttttctt cagcaggagc
    74161 tgcgggttcc gctggtgctg ctgtctgtgg gctgcagcct gcaagtccga ctgaaacagc
    74221 catcagacat accaatagta atgccagtac tttctttttc ataatcttcc tcctccaaat
    74281 ttctgataat taatttagaa ccatatcctc ctttcttaat aacgcatcag caggaaattt
    74341 ttcgattgca gaaaaattta ctcgcctctg cgcatttcaa cgaggcgtga aaaatgccca
    74401 aacctgtcgg agacatcttt ttgcctcgct ataatacaaa tatgaatatt attgtataaa
    74461 tatatttaaa aaatttagaa aaatgacatg taagctgccg gtaaaatttt atcatcagaa
    74521 taattatttt attctagcgt atgtcttaag attctacata aattaatttt atccttctaa
    74581 aaaaagcctt ataaataaaa aatacactat atggctatag tgtatttttg ccgcaaatga
    74641 tgattacatc agctttgtcc cctgattttc aacaacatcc cgttccttaa gcatcctgat
    74701 aatcttgctt gcatcacttt ttgaaagatc tttgctgaag ctctttcccg tcttatcctg
    74761 tatcaaatct ctcagttcat catttgatat attaagattt tttgctatcg caaatatagt
    74821 attgatttgt gtttttgtag cgccttcata atttttgtgt tcctcggcct gctggctctg
    74881 attctcttca cttatgcttt tcccttcccc cacctcctca aatcctgtat attcaactcc
    74941 gtagccggca aatcgcaatg ccctggcaat tgctcttgtc tctgccagct caagaattgc
    75001 atttgaaagc accttatccc tctttactga cgcattcccc aatccgttaa agattccttt
    75061 tccggtcttc acggcagcct ggaccactgt tgattcctca ttatactgca ctacgctggt
    75121 ttctatggat aaattctcgt tctcatcatg ggcaagtctc aatctgccgc caaccacggg
    75181 atagattttt ccctgcacct ttacaagttc ttcattccta aatgtactcg gcatattatt
    75241 ccctcctgaa tatatattgt gagggtaaga aaataatatg catatatctt cagcttattt
    75301 cctttttcag ttctgcaatg ctttcctcaa gctttttcag atgatacagc aatgaatcat
    75361 tatttgtatt gctgccttca gaaataatgt attcaagact gtcaaaagta tcacccgatg
    75421 cagcctttag tgcccttaaa tagcttatga gattatgttt attcatgctt cttctttcaa
    75481 ctgttatttc cctggcagct gctgaagata gctctaaagt ctccccaagc tttgcggctt
    75541 caacaggaat ccccagctct gattccataa gcttagacag caccttcgac gattcttctt
    75601 ctccatgcac cagaaatact ttttttggct tttttataaa agaccctacc cattctataa
    75661 gccctctctg gtccgcatgg cttgagaacc cctcaataga atagacgtta caattcacac
    75721 tgatttcttc accgaatatc ttaacaagct tttctccgtc ctttattctt cgtccgagag
    75781 tattttttgc ctgatagccc acaaatacca cacttgattc ttcgcgccat agattatgct
    75841 tgagatggtg ctttattctt ccggcttcac acataccgct tgctgaaatt ataatactgc
    75901 ttccctccat tgaattgata gccttggatt cctcagccgt gtgggtgaat atgagattag
    75961 gaaaatcaaa gggattatct ccttctttaa gcaatttctg agtatcctca tccatatatg
    76021 gcagaaagct ttgaaatatg tttgttgcat taattgccag aggactgtct acaaataccg
    76081 gtacattgca taatggtaat gatttgcatt ccttcagcat attaagtata tacagtattt
    76141 cctgagttct ttcaactgca aaggaaggta ttaccacgtt gccgttcctc tctattgtat
    76201 ctgttataat atccagcagg gccagagtct tgttattcat ctccatgtga agcctgtccc
    76261 catatgtgga ttccataatt atataatctg cttcttcaat tatcgtcggg tcattcataa
    76321 tgggtgcatc tttatttcct atatctcctg agtataccag cttaatctct tcgctgcctt
    76381 ccctgatcca taattctata attcccgacc ccagaatatg tcccgcatcc attatcctga
    76441 atgaaatgtc cgggtcaata tttataatct tattgtactc cacaggttga aagaatttta
    76501 atgatgcttc tgcatccttt actgtatata aaggctctgc aactcctttt cctgctcttt
    76561 ttcttttcct gcttttccat tccgcttctg attcctgtat gtatgcactg tcaggaagca
    76621 ttattttgca tagttcaaaa gtcggggcag tagaaatgat cttacccttg aagccctgtt
    76681 tgacaagcag cggtatcctt ccgctgtggt cgatatgggc atgggaaagt ataagcatat
    76741 cgatttctga aggattgaac ctgaatggtt caaaattcat ttcctcagct tcacttccgc
    76801 cttgaaacat cccggtgtcc agaagtatct ttttatttcc cgtctctata agatgacagg
    76861 aaccggttac agtacctgcc gcgccgcaaa aagtaagcct catattacac ctccgtaatt
    76921 actcaaatta tacacatata ttgactatat tctacattta ttctctatta cctttataaa
    76981 atcaaaaaga cctgtgaaga aaacaatctt caaggtctta ttcttgaaac attggaacca
    77041 aattctattg acaataaatt catttcgtta tatcatttat attgtcagtt tgcggggcgt
    77101 agcgcagctt ggtagcgcgc ttggttcggg accaagaggt cgcaggttca aatcctgtcg
    77161 ccccgaccat tgaaactaca gctcaataat atcagggtca taatccaaat ccaatccata
    77221 aaaattgata tagcaataaa tgaactctct tttatactct ctgttcctta attgcctttt
    77281 cagctttttc aaatacttct tttcgttcca ctttttattt gctatatacc gttttctgcc
    77341 cagcttccaa aacttgtgag ggaaaaccaa tattgccagc agcatttcat attcttcctt
    77401 tgacatgggc cgaagcttgc aatagctcgc aataagcctc ctgcacaagt caaaatccca
    77461 tttgaacctt ttcttcgaga gtatccttct tatcatttta cctaaatcgc tcatgggaat
    77521 atcgtattgg catgactcca ggtcaactac aaacagttta ttgtttctgt caacaattat
    77581 gttttgataa tagaagctgt cgtggcatat actgccttca tttacataat attcgcaaag
    77641 ctcattgtac ctgctgtggt caagtattct gattgcaagc tcggcttccc ttataaaata
    77701 gtcaatattg ctcttatata tgtaatcgaa ctcggctttc agcttaagct tatcgatata
    77761 ctccctgtat cctgccagtt catcccggca ctttgcgaag gttcggcgcc actttttaat
    77821 atgggactta accttcacat gcttaggcgt cctgaatcct tttgccctgt tgtgaaaatc
    77881 cgccagaagc tcggaatatc tttccatttc atccggtatc gaaaagcttg cctcgcatcc
    77941 ttcgatccaa taagtaaggt aaaaagccgt gctcttacgt tttattattg gctttccttc
    78001 ttttgtgaag taatattccg caatgtagtc acatccgttt tcttttaaat gcagcattat
    78061 attatagctc tttttggctt tttcataacc attgtttacc cgtttcaagc agaaaaaacc
    78121 tttatccgtt cctaccttat atgcacttct gactttatct atcgattcta cccgaaaatc
    78181 ataatggttt aatacaacag caagggttct catttctttt tggtctatat ccgatgtaca
    78241 tttgctttcc agctgtgaca gcattatatc cactccaatc acaatatgta cctataattc
    78301 cggttaatat attatatttt ggagtgctat gatatatatc aaaagaaaaa acaaacttgc
    78361 cgattggcaa gtttttaatg accgctgtta cacttcctat ggttgtccgc tttttttgta
    78421 gaagaaagtt tttattgact ccagtttata tttatatgga agtattatct gctccgtgct
    78481 gccgacaaac aacactccgt tgttgcttaa ggaattcctg aaattatggt acattgttat
    78541 cttagcttct tccgtaaaat aaatcattac atttctgcat acgatcagat cacagttttc
    78601 ggggtatttg tccaccagca gattatgatg cctgaaatct atacagttga ttatcctttc
    78661 acttaccttg taataatctc ccgattttat aaaaaaacta tcaacaaaat ttcttggcag
    78721 gttctcaagg ctcttcgcgg aatatatccc caccattgcc ttattcagcg cagcatcgtc
    78781 tatatctgtt gcaagtatcc tgaagctgtt cagatcaacg tatctgctca tcatcattgc
    78841 aagagagtag ggctcttctc ctgtagaaca tgcagcgctc catattttag gctttttatt
    78901 aaatttcatt atttcgggca ttatttcgtt ctccaggacc ttccactgct ccggattcct
    78961 gtaaaattca gatacattaa ttgtaagata attgataaat tcatcgtata gatttgaatt
    79021 ctgtgtcaat gctttaaaat actcatcgta atcatcatag ttgttcctgc ggataaggga
    79081 gtctatcctc cttcgcatct gccgctcctt gtagcttgac aaatctattc ccgtaagctt
    79141 taatatcttt tgtttaaacc cttcatagtt ttctagcata agcaactcct atcagattaa
    79201 taataagcta ttactattct atcatatgaa aaacataaaa aaaagtcgtg atatgtttta
    79261 tatcacgata atataatgta aagggggtct caatgtattt tgtgaggtcg atattagtgt
    79321 tgacaatata aaaatattta tacataaatg ttaaaacaaa ttaaaacagc acacttcttg
    79381 tgaatatcac aatagtgtgc ttgtattatg tttattcttc ggcagtttct tctgcggttt
    79441 cttcagttat gttttccgct gctgcttctt cagcttcctc agctattggc ttttctgctg
    79501 cttcttctgc ttcctgcttt acttcttcaa taactgactt ctcagcttct tctttagctt
    79561 cctcaacagc cggtttttcg gcttcttcct taacttcttc aattattggc ttttctgctg
    79621 cttcttcctt agcttcctca ataaccggct tttcagtctc ttccttagct tcctcaataa
    79681 ccggcttttc agcttcttct ttagcctctt ctccaattgc ttctattagt tctcttatac
    79741 tcaagcttat tttcttttct tcaggcttta tatcaactat ttttgctttg acaatttctc
    79801 ctattttcag agcatctgaa ggctttgcaa ttcttttgtc agatatctgt gaaatgtgaa
    79861 taagaccgtc tatacccggc tcaagctcta caaaggctcc gaagttcgaa aacctcaata
    79921 ctttaacatc aagaacatcg cccggcttat acttttctcc cgcagcagac cagggctccg
    79981 gaacagtctg cttaagccca agggaaatct ttttcttttc cttgtcaacg gataatacta
    80041 ttacttccac cttatcgccg ggttttacaa tctcagaagg atgtttgact cttgaccagg
    80101 aaagttcaga tatatgaatg agtccgtcta ctccgccaat atccgcaaag gcaccaaaat
    80161 cagcaaggcg cttaatctca ccggttaatt tctgtccgac ttcgatgttt tcccataacg
    80221 ctttttcttt aatttcattt tgctttttca aaatcgactt tcttgataca acaacttttc
    80281 ttttctgcgg atcaaattca ataatctcaa cattaacttt cttatttaca aaactgctaa
    80341 gatcctctga ataccgcaca tcaaactggg atgcgggcat aaacgccctg acgcccatga
    80401 gttcgactat tcctccgcct ttaactattt cctttacaaa accctccaga ggggtttttt
    80461 cattaaatgc cgcttctatt ttattaaagt tcttttcggt atccagaagc ttcttggaaa
    80521 gcacgacatt accttcaccg tcatttattc ttaccaccat tacttccaat tcgtctccgg
    80581 cttttattat atccttcagg ctaacatcag tatcccaggt atagtcttcc ttctttatta
    80641 ttccgtcgga tttatatcca atgttaacca tgacctcatc atcggtaacc atgattactg
    80701 ttccttttac tgtgtctcct ggatataccg gcctcatggt cttttcatat tcttccatca
    80761 tatttccctg gtcttctgtc gtatcttcct gcttctccat ttcttcccca tcatccacta
    80821 cgctttcctg attctccatt atttccaccg gatcccccat aatattgccg ttgtcctgca
    80881 ttttttcgat aacctcctta attaaccaat caggtgtgga tgcacccgcc gtaataccaa
    80941 ccgtctcaaa tcccttcagc attcctgccg gcaaatcctc cgctttctca atatgataag
    81001 cattatcgca attctccagg catatctgat aaagcttatt agtattagaa ctctttctgc
    81061 ttccaattac aatcatacaa ttaacttttt ttgaaagctg ttcagccgat tcctgccttt
    81121 tctcagttgc gctgcaaatg gtattaaata ttattgcatt tttatatttc ttattaatag
    81181 catccactac gctgtcccag attttttttg taattgtagt ttgtgcaact aaacatattt
    81241 tatcatatat aggaagtttg tctactgttt tttcatcctc aattacatgg gcggaattgt
    81301 cacaccaccc atttatgcct attacttcgg gatgagcttt atctcccgca ataaatattt
    81361 tatagccctt cttataatat tcctgcacct ttttatgtat tgccgataca taaggacagg
    81421 tgcagtcaat atatccgaat cccttttctt caatttgcct gtactcttcc tcacctattc
    81481 cgtgtgaacg aataataagc tcggcacctt cacaatttat ctcatcaatg ctgtccgcag
    81541 attttatacc caattcttca agcttgcctg tcacctgatc attatgaata agagggccaa
    81601 aagtaaaaat ccttctgtcc ttctttttat ccgcttcttc aaaggcttta tccacagctc
    81661 tttttacacc gaaacagaat cccgcatatt tatcaataat tattttcatt ttccctatcc
    81721 ttctttagct cattaattct tttcatgata tcgacactta ttttaatata ctcgtcattc
    81781 aaaagcttct tatcatagaa ttctgcaagc tcaataggct cccctataat tatatttgtc
    81841 cttttgaaaa acttatagct gctgataatt gccaccggta caatcggaac ttttgacttt
    81901 attgcaagca ttgcaatacc gggctcagca gtaattccat cacttttctt ccttgttcct
    81961 tccggaaaaa gagcaagaat ttttccgctg ttcaacagtt taagagaggt ttttatgctc
    82021 ttaatatccg cttcccctct ttttatcgga aatgcgccca gggcgttcat caatgcccta
    82081 agcagtctat ttctgaataa ttcaaccttt gccatgaagt acatcctgtt tggtatcgaa
    82141 attcccacaa gtatcggatc tactgcactt acatggtttc cacagactat atatgggccg
    82201 tcttttggaa tgttttcacg gccggttatt ctgtaattat aaaataataa aaataccggt
    82261 atcagtaatg acctggcaaa cctataaaac atcagatcct cccccctttg attaaatata
    82321 tgaaactact tcactcaaaa cctcatctat acctttaccc gtcgtatcga ttattatggc
    82381 atcagaggct gctttcagcg gagaatcact tcttgtagaa tcaatataat cccttttttc
    82441 tatctgtgac ctgatatcct gaatctcagc ttcatagcct ttctgcttca gctctttata
    82501 tcttctgtca gctctttcct caacagaagc tgtaatataa aactttacat cggcctccgg
    82561 aaaaatgact gtgcctacat cacgtccatc cattaccaca ttcttgttca tggatatctt
    82621 cctctgaata tcgactaaaa gcttccttat ctcagatatt gccgcaatat atgatacatt
    82681 tctgtttaca tcctcttccc ttatttctgt attgacagct ctgccgtcaa gatatatgct
    82741 gttatcccga aaatctatat cagttttttc agccatgctg atcagcatat ctgttttttt
    82801 caaaggaata ttctctctca gcaccttcaa cgttattgcc ctgtacattg ccccagaatc
    82861 tatgtaagta taacctatca tatctgcaag cttttttgct atggtgctct tgcccgctcc
    82921 ggcaggcccg tctatggcta ttactttata accgctcatg gataatcccc ccaatacttt
    82981 gccatttcaa ttacgccttc actgatgata ccattttacc agaaaggatt tcaaaataaa
    83041 agattattgg tcacagatac gattctactt tattcttatc ctgtctacca tcacaagatt
    83101 atctttcaca tatacaagct ttcccggttc gggcaccgtt atgctgtcgg atacagccgt
    83161 aaccttaaga actgccgtat cattcaaggg gccgcctcct acctcaataa tatcactgtc
    83221 ctttactttc agccttattg atcttccgtt cggagtgtca atgccttttc cgtttatata
    83281 aaaaaccagc ggctttaagg ttgaataatt ctctatggat atctcaagct ctcccctttt
    83341 aataaaaagc tgactgtcgg ctatggacct gccttccagc ttgtctatcc tgcttataat
    83401 gttttttatt gtctggctta acagcaatgc ttgtgaaatt atgagagctg caaataaaag
    83461 aagcagaact tttctgatta aaccgtcaat cctgtcccac cagctttgga aactgctgtt
    83521 cactatcttt atcctccctc atatgtattt atttagataa ttcctctttc gggaggcatt
    83581 tattcagtta tgcaaaaaaa cccgccgcat agccggtgga aaatgcaatc tgcagattga
    83641 agcctccggt aaaagcatcc agatcaatta tttcacctgc aaaatgcaat cctttaagaa
    83701 gctttgactc cattgtccta gggttaatct ccttcagatt gatacctcct gaagtgacta
    83761 tagcttcttc tataggcctt gtgcctgata cggtgactgt aaacccctta agcagctttg
    83821 ccatgtcctt cctttcctgc ttggttatct ggtgaacctc ttttgactcg tctattcccg
    83881 atcgggatat tacaaccggt atgagttttt gcggcagcaa ttggtccagt gagtttttga
    83941 actgtttgtt tatatttttt tcaaaatccc ggattattct tttgtcaagc tcctcctcgg
    84001 aaagagccgg tttcaggtca atgctaatcc taatttcctg cttccttctg agataatccg
    84061 aaatgaaaaa actggcagac aaaattattg ggcctgacaa tccatagtgg gtaaaaagca
    84121 tttctccgaa atcctcatat aaaagcttcc caccggcgta agctttaatt gcaacatttt
    84181 tcagggacag tccctgcagt tccttaacaa atgcatcatc tgacaccagc ggcaccaatg
    84241 acggcctggg ctctattatg ctgtgtcctg cctttacggc aattttatat ccgtctcccg
    84301 ttgaccctgt attaggatac gatagtcctc ccgtgcaaat gattatagag ctgcattcca
    84361 caacccggcc gctgtcaaat aaagcgcctg ttacagcacc gttttcctgt cttatgtcca
    84421 ggagcttact gttgagcctt acatcaacca tattcctatt caaatatctt tccagggctt
    84481 ttataacatc attggacttg tcggattcgg ggaaaaccct cccgcctctc tctacctttg
    84541 ttttaagtcc cagggattcc aaaagcaaaa taagatcatt gtttgaaaag ctgctgaagg
    84601 agctgaagaa gaattttgaa ttcttcggaa tattcttcat gaattcatca aaatccgcgg
    84661 tatttgtaac attacatctt cctttgcccg tgataaacag ctttttgccc agcttctcat
    84721 tcttttccgc cagcatagtc ctttttcctt ttgaggcagc cgtaccggca gcaatcattc
    84781 ctgcggggcc tcctccgaca actattacat catatatcaa gaaaaacatc ctttcaacca
    84841 tattatctca aacgcagaat tataatatgt ttaattctgc tatagatttt cttcctccaa
    84901 gtgaccggta tcattcaggc atattactac aggctttcct gagctattct cagggaattc
    84961 cagtattgat agtgatgcgt tgtctatcat gaatttttta aagtcagcaa tgtctaatcc
    85021 cagtattctg attatcactg ctttaatagc tgtgccatgg gataccagca gtatattgct
    85081 gcctttatga cgggtgacta tatccataac cgcgccatat gtacgctctg tcagatctat
    85141 gagggtttct gccccgggca gcttgaaatt aacgggatcc tctttatata ccctgaaatg
    85201 ttcaccgtat ttgtccttga tttcatttat tgtcatgccc tcccaagggc cgaaacatat
    85261 ctcgtgatac ttatcggatt caatgatttc gcattgctta tattctgcaa taatttccgc
    85321 ggttttcaaa gctctcttca ggctgctgga atatatcacg tcaatatctt cccttgccag
    85381 ccttcttgca agcagcttcg cctgtattac accttcgggg ctgagatctg tgtctgttct
    85441 tccctgaacc ttgccgtttt tattccattc agtttctcca tgtcttacaa gataaagcct
    85501 ggtcatcaaa tccctcctta aatagccata ttaattatat catagaacaa taataatgcg
    85561 tcgtaatgag tttgtgaagt actccgactg taggggaagt atgttctcc
//
LOCUS       DAOQUR010000005        77484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_379_length_77484_cov_10.480983, whole genome shotgun sequence.
ACCESSION   DAOQUR010000005 DAOQUR010000000
VERSION     DAOQUR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 77484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77484
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_77484_cov_10.480983"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..3286)
                     /gene="carB"
                     /locus_tag="PKI50_01940"
     CDS             complement(62..3286)
                     /gene="carB"
                     /locus_tag="PKI50_01940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNT02509.1"
                     /translation="MPKRTDINKILIIGSGPIIIGQACEFDYSGTQACKALRNLGYKI
                     VLVNSNPATIMTDPGIADSTYIEPLNTKSLTEIIDKERPDAILPNLGGQTALNLCLEL
                     YKSGILDKYYVKVIGVQINAIERGEDRIAFKETMNRLGIDMPCSKAAYSVSEAEKIAG
                     ELGYPVVIRPAYTMGGTGGGLVYNAEELRTIASRGIAASMVGQVLVEESVLGWEELEL
                     EVVRDSKNQMITVCFIENIDAIGVHTGDSFCAAPMLTISPELQQRLQEYAYSVVESIE
                     VIGGTNVQFAHDPRTGRVVIIEINPRTSRSSALASKATGFPIAYVSAMLAAGLTLDEI
                     PYWRDGSLDKYTPSGDYVVIKFARWAFEKFPGAQDKLGTQMRAVGEVMSIGKSYKEAF
                     QKAIRSLEINRYGLGFAGNFNNNTLYELLSMLTEPTSQRQFIMYEALRKGADVEQLYK
                     LTHIKPYFIEQMKELVLLEEEILKCKGSELQDELLIKAKKDGFSDRYLAQLLDVSEAL
                     VRRKRTGLGLTEGWEAVPVSGVENASYYFSSYNISDKTSVTDKPKVMILGGGPNRIGQ
                     GIEFDYCCVHAAFELRDLGYETIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIYEK
                     EKPLGVIVQFGGQTPLNIAGDLEKAGVRILGTSQETIDLAEDRDRFRRIMDKLDIPMP
                     ESGMAVNAEGAIAIANQIGYPLMVRPSYVLGGRGMQIVHDEEMLCTYMAAAVGVTPER
                     PILVDKFLGHAIETEADAISNGADTFVPTVMEHIEYAGVHSGDSACIIPPVGISEEHI
                     STIVDYTGRIAKEMNVIGLMNMQYAIADNKVYVLEANPRASRTVPLVSKVCNIQMARI
                     ATRIIMADCLGGRYSLDGLASKKIPHYGVKEAVLPFNMFPEVDPVLGPEMRSTGEVLG
                     ISDSFELAYYKAQEATQELLPSLGTVLISVNDADKPDVLEAAREFSRIGFKIVATEGT
                     CKFLQEKGISCEKVNKVYEGRPNIADNMENDEINLIINTPQTRLSEFDDSYIRKIAIK
                     RKVPYITTMTAALASAKGIAAYIKNGFHGKKIKSLQEYHRDIAE"
     gene            complement(3587..4393)
                     /locus_tag="PKI50_01945"
     CDS             complement(3587..4393)
                     /locus_tag="PKI50_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNT02510.1"
                     /translation="MNFLSGFFKGLLIGIGGVAPGVSGGAIAVIFGLYERITDAIADV
                     FKDFKKNAVFFFPIALGGGVGVLGFSKIMQYLFQYYEVQVKCLFIGLMLGTLPTVFRQ
                     ANKKGFKGIYIIPCIIAFGITIITVVLENAGIDIIPDAAPGMLQLVLYGAIIGVGTIV
                     PGISASFVLMYFGAYQIVLDGLANINLSILVPVGTGFVFSIILFAKFISLLFKKAYGF
                     TYYAVIGFVIGSIISIFPGISFSSEYLISILLFIMGYIITFLISKYSKDT"
     gene            complement(4452..4973)
                     /locus_tag="PKI50_01950"
     CDS             complement(4452..4973)
                     /locus_tag="PKI50_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT02511.1"
                     /translation="MDFLELAKMRYSVRNYLPRKVEEEKLLKILEAARVAPTGSNRQP
                     HRLIVVQEAESLEKLRKSANIYGAPLTIIVCADHNISWKRSYDGKDVADIDAAIVTDH
                     MMLEATQLELGTLWICHFNPKVLKAEFSIPENLEPMNILAIGYSADAALSPERHQELR
                     LPLEDFVLYESYK"
     gene            complement(5072..8074)
                     /locus_tag="PKI50_01955"
     CDS             complement(5072..8074)
                     /locus_tag="PKI50_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT02512.1"
                     /translation="MDFPISIVLTSYSGVGPQEIENIITRNIESAVATVNNIKTIQSQ
                     SSEGQSIVIAEFNSGTDMNFATLQMREKIDMVKGMLPDGVEAPMIIKMDPGMIPAAVL
                     GVSGGENEAELTRFVEDKIKAKIESIDGVASVAVTGGTTKEIKVNLDSQKLSGYGLSF
                     SQISGALRSENLNQPGGTVEYGDKSLLVRSTGEFQSLDQIRNIPITLPTGAVVYLRDM
                     AEVKEGYKSVDTYNRMNGESSIGLTVQKQTDSNTVKVVNLIKKEINNILKEYPDVKVE
                     MVFDQGEYVEQSIANVAKNAVVGAILAIIILFVFLKNIRTTMIIGTSIPISIIATFVM
                     IYFSGITLNLVSLGGLALGVGMLVDNAIVVLENIYRYRNEGHSRVEAAMLGTQEVGSA
                     VLASTLTTVVVFLPIVFTGGIAAEIFKQMALAVTFSLIASLLVALTLIPMLSSKFLKM
                     VKYHEASRSERLNKVFDKWEDTLSAVDNFYRSILSWALAHRRKTVITVLAIFAGSLLL
                     IPFVGTEFFPNMDMGQFTVGIELPQGSLLKNTNEVAEQVEGMLNSLPEIQKVFTTVGS
                     SGSGMDLSGGASNTASLNITLKPLSERKRSTAEVVEEMRNKVRLIPGADIEINEVSTS
                     AMGVSGGTAVSVKICGPDLDKLSEIAADAEELIKKVEGVRQANTSISEGRPEAQIYVN
                     RDKASYYGLGTAQVSAAIRTAVEGSVATTYKVDGDEIDVRVGFPEETGEKYSQLIDTK
                     ILSPLGVEVPLTEIADIKMEKGPISITREGQERYVTVSSDVFGRSTGDVNKDISERLD
                     EMLLPPGYSIKYGGEAEQIADAFSSLTLALLLAIVLVYMVMAAQFESLLHPFTIMFSL
                     PLAFTGSVLGLVLTGRALSVPAFIGVIMLAGIVVNNAIVLVDYINTLRGRGMDRLEAI
                     LKAGPTRLRPILMTTLTTILGLIPLALGIGEGAEAQAPMATVVIGGLTTSTVLTLVII
                     PVIYTLFDDVSVKLRRHKAGSIRKTEAV"
     gene            complement(8204..9460)
                     /locus_tag="PKI50_01960"
     CDS             complement(8204..9460)
                     /locus_tag="PKI50_01960"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT02513.1"
                     /translation="MKRKYTKLIIGIVVVTLLGSLIFTYVKNKAGSSANINADTVVAV
                     SVEAAAKTSVSATVNLNGKLKPIQEINIIPKLPGKVNNIYFDIGQSVKAGDVLFTLED
                     NDIRSQLKQAQAGLDMANSGLAKVTGGGAELQELQLKAALTSAEISYNDAKLAFERTK
                     QLYESGAVSKATLEQSESQFKLMEQQYNSAKSNYEITTTKTNPENIASAKAQVSQAAA
                     ALELAKSQLDNTVIKSPINGVIAARVIEIGELAGSTGIAMSVIDLSSVLIDINATEGM
                     INKIQVGDKAEVTIKSVGDSKFTGEIINISPTIDTRTQSYPVRIKIDNSSGTLKGGMF
                     AEVKTVLDKVSDVIAVPISSVIEEGDKRFVYVLNGDIAEKREVSTGLFDDELIVIVKG
                     LAENDAVIVKGQELIIDGSKVIVTEN"
     gene            complement(9486..9932)
                     /locus_tag="PKI50_01965"
     CDS             complement(9486..9932)
                     /locus_tag="PKI50_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT02514.1"
                     /translation="MDIKDKCDRMMKIAMDIHKKLMAVILKDIEPSLSKSHVYLLAYI
                     QRKGECTVTDIANHLEITLSAVTSLVDKLCTAGLVTRLRSVEDRRVVYMELTPEGEKV
                     LEKIEANKNRLSEKIFSNIDTEEINNFFATIEKITQNIIDSYSSEN"
     gene            10150..11133
                     /locus_tag="PKI50_01970"
     CDS             10150..11133
                     /locus_tag="PKI50_01970"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNT02515.1"
                     /translation="MLYIRNENTNPFFNLAMEEYLFNLNDNNDYVLLWQNEPTIVVGK
                     YQNTAEEINSEFVKEKGIHVVRRITGGGAVYHDLGNLNFTFINKGTGKKEFDFRKFTM
                     PIVKALERFGVKAELSGRNDITIDQKKFSGNAQYVKQGKVLHHGTLLFNSKMEELIKA
                     LKVTEDKFQSKGIKSVRSRVTNIADYLSENITVSEFKELLLKYMFDEDTELIEGQLKD
                     ADMNEINLLMKSKYMNWDWNYGASPEFNVKQAKRFEGGKVEVLINIKNGVIQSIKFYG
                     DFFGSESPEEIEALLRGKRYNEDEIRGAIAPLNINEYFKGISPEELLSCII"
     gene            complement(11650..17679)
                     /locus_tag="PKI50_01975"
     CDS             complement(11650..17679)
                     /locus_tag="PKI50_01975"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT02516.1"
                     /translation="MRRRLKSALMLVIWTLSIFNGIALEAVNAATPKIYSIKNDKIEL
                     LVNGDDGRYAIKTVAGSPNRPEDDNKSLLYLKGIPESTFTTFRIDGKDYIYGNSYSGL
                     TMDGGFTDTPKTEGMVNTSTWKPEGIEITQKLEMTDNVTSSDVGNVKISYIITNKGTS
                     SKLIGTRILMDTMLGDNDGSKVSLDGKSDISFETEVIGEDIPVYWRTVDSAENPKIVS
                     YGFLKGWGNTEPDKMIIAHWSSLSSTKWDYSINRDRNISSILNDYKSADSAVALYWEP
                     KSLEPGETMQVETYYGLGNIGNSDGSTFNMNVLVPSKLTIEGDKYKGNPFEIIMELDN
                     SLPNSTQLAGITAELILPEGLEIEEGEESSKYFYKINISGKETVKWKVRALNSQKLRV
                     LQYMVRITGMSQELKTIKKFIIIPGFENNNFEIGYTDAVPRNLYYEDENNAIQLIGHG
                     FDKLKDKSGYEMGFMDKKGSIVHYFSPEDITIINDNQIRLKVPKGFAVGTYRISINHR
                     EDILDYTLPQELFLTSDIKYKSRNYGIMAIKEDNGGVQSVKLYENESQLTPAEKSNSK
                     LIIRGKIKDISADNYEIYGDTIAINNNIYYKGYGDNVLSVHKSGQGYAVKGNGELYMQ
                     SCLMGSSMEVTLKKGYFLIDSATARIRDQEGEFNDITTIKVGYFPIIVKEIKIQNNGE
                     VRIEGLLQLENKYFSFLTGLGTGSVKSDLKDLSLTNKKIDIDAEIEIPFPRWKLGGFQ
                     SQNYATKSMTQITFFINTIKGAYGFETKAENIKLRLLDINAKMAFDKNLYPDYFEFSN
                     NYGKIPKPIGSTGLGFERIGGGIYGLKSMIDSLKYGILPTGSSIAVRANIVDLFTYHA
                     RIKGYTLIGLLDTEAILSSSGIDLEGDAYIYFIDVGDIIGHFDFSGGYIEADLNVLDI
                     LIADAYFGISSHEIKGSINAKVQVPDYVWFIGGEEIAGFGAELSTEKIKGSISFMGVG
                     VGITYTWKDHDVDFDVGSNSDADDKGIYTVRTKDNRGRDVTVTYGSNIERLKEVEPSY
                     PSHNVCYAGSMGKILAGTFEYNYDVTIDDSFESAIIEMKYTSSETPDIKVTKPDGTTY
                     ALADGVNYRNQIIPADKSASGEEEKYLFVTIPSPITGEWLIESDRALAVTPCKVKAPA
                     VFESISAEQSAGKIRTTWELNVTDDSTVSLYLVKEDGTSGMIELAKELPGGLGTFECD
                     IPASATTGMYKVRGEVRRTGIDAEDTFSFDEMNTAAFEIVDAEAPDTPTGFTVKSAGN
                     GMMEAEWNFSDGADEYRIYPVNESNKIDNTVKAMVSVEGTETSTIFGGTQIIPKKSED
                     DETETSPEELEYGWIPGRSYRFAIYAVNKSTVSDPEDPDKDMEIEHISRPAYTGSVNL
                     PVPEPPEFTASFSSEHGNINVEKDENDIDIRYTNYDAVICSYQSDINADVVFYVNGDD
                     VADFTGKEIIQELELNDGSNLIEIEAIKANGDKTLKSYEFYYDNKAPDLMVQSPNSSD
                     NIKSGKVLVSGKTTPGSRLYVNGTEMTVKEDGSFNEDFILSDTQRETITIAAVDLACN
                     RTEYSTEVFNSDLGDVVRVQIIPQIEQLRVGESIQLKLYGITNNSSQVLLEAEKSDWQ
                     LLDTGGSATITPEGLLTAVKPGEAIIKGQYSLSGDMSYEDALIINVVSDASNRGDDED
                     SREDYPKATDTNGINAGSLLRKSLDFNANDEITVPGLIQLRFTGNEIFANGHLEIYEI
                     KDLLQYRQQSGNKDFISSIMDIRIPEGYQFNSPIELTIIFDRNKVKDIKKIGIYVYNE
                     KTGDWDLVGGTVDEGKGTITVIIPHFSKYAVMKNSGMAVMTDMEGHWARDAVYRLIDR
                     GIVNGIKLPSGDYRFEPERTVTRAEFAKMLALCEGSPQTGVNTDLSGFADDSDIQPWT
                     RPFLKYCSSNGWINGKGIGDKVYMKPQDPITRAEAAAMVSRALKLSAADKGIKAEFSD
                     KDKIPQWAAVYIDQLIKKKLMQGYSDGTFRPDRVLTRAEAAKIFDIYISLQ"
     gene            complement(17692..23613)
                     /locus_tag="PKI50_01980"
     CDS             complement(17692..23613)
                     /locus_tag="PKI50_01980"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNT02517.1"
                     /translation="MKNIKIKLFLFLTLMLTTCWALSGINVYGGWDIYLINDDGSSPG
                     SWEHVINKLASATSLKNGPSSGNPGSFNYFKHAGDGTREMSDAVISKTVTLNADQRTL
                     CSLNQLQVNFLLDYFGWDSDDEDRFVVSIYSIKADGKWSTIFNSSSYVGEKYWRRLNM
                     PTKALPPDTVKVEAVITAFRESGSDLDVYLDNILLWLVDENPPVMTEATVTEIRDWQG
                     NLVPLKKDTVSGEYLDNWVNPADTIYGNIRFNEPVRVNYPEVTLNTNVLNQYGNRVYA
                     EIDSTSWHYTAHSTSHQYSIPLGHKCQISGEDNKIKFMDSLESIGAAGPFDYAVQDLG
                     GNQGPSDIMKPNIEHYSIKFDNASPVIVTSWDSYEEYAPGRSSVEIVIEEEEMGREQS
                     PLTMTYSWQYMNSTGFAVREEEKSIVLNDATAHRVSDGTKTTYTVTLAIPNGSKIPPY
                     QEFRLYAEVNDEARSPYGNRFFYGKVNQKDSTPPVITWDKSIHEDGREVDISTSEDTL
                     YTTSRTVSFSAEDIESGVDEVKYIWTREAYNIDSSSFKKVVLPGEDGKYTIETVREDT
                     LLEGIYYLNILAVNGTDEEVIIRKGFYFDCEGPRVTYSEFKDIDGKPASAHYQVKDRA
                     LQNKFLYALLTTMEDGILDFEPIAEPDISEGIKDSGMWKVLDLEGTGEERTANIMGVL
                     DGVELSGYYKLVTRYYDEFYNYTEYGNTIWYDFEPSELILMGSADGEPFEPENYKRDH
                     SVKVHVIDNISGANLLNEEVSVNWIDADSREVISADFETENDQFPGIKVYGNENLNGI
                     YYLKIKVKDYAENVLEEILSIDGNPVEFCFDNSPPEVTIVCDASKKLNTVMFSYSGLK
                     DEYSDISLFGFGISDTPGGEPETWIDIDTISDSGEVIHFGDVAYPEDSITEGKWYLSV
                     MLQDALGNMQIVHCPEVFDIDATKPAGSVSFEKDFTNSLEVSLKLTVDELAEAGNKVF
                     KTVLADDGSLLSEASIDDEAWKDIIFENGTAYFNWMLTNTDEAEQSVFVRFMDEVGNI
                     SDIYSDLITLDRTAPIGSVTYDVTAATKGDVKAALTTEDDNPVIILNNNQSAEYVFNC
                     NGEFEFIISDEAGNKTRVKAEVSNIDKDPPKAAVKYVWTEEPSIIASIDQWTNKSITA
                     QLQMEDENGCVILSEGGDTHTFNENGEFTFEFRDNLGNEGSIKAEVKNIDNTPPVGSI
                     IYTPSDTAPVTVYLSVNEPVKVTNNNESFRHVFEENGSFTFEFEDKAGNTGTATAVID
                     TITQAEKYADIIYSDAGRLTNENIRAGFKAFSGKAIITSPTVAEEVYGYTHEFTENGE
                     SPVSIRVLSGEDEGYIRTVAGSVYNIDRTAPEAEVILSETELTNQNVTAALLTYDDRG
                     KTVVIRNNNGSNTLEFTENGEFTFEFEDEAGNTGYKTVTVSNIDKTAPSGEIEYIRDT
                     DKNIISAKISFPEETEEVTILNNNGSDTFEFVENGAFAFEYSDAAGNKGETTAQVTGF
                     AGTAAPSAIEYYIDGNKVEDPNLEPINKSVTAKLIPEAAGGSYTIANNGGSDSYVFQQ
                     NGEFTFVYEDVTGNRGFAKAAVSKIDKSAPKLQILADIVYPTQSDVTLTVSYSDDKGT
                     VYVDHNMEAENLTASEGKLTYICTENKTIRIKVSDAAGNETTKEFIVDYIDRIAPVGN
                     IEYTPGSITNQDVRAVLNLEEPGKVLNNNGKIEYMFTENGEFTFEFEDEAGNRSSKTA
                     SVNWIDKLPPKGSLEYSHTEMSNRPVVVTLKADSDAAIINNGGIPARTFYTNGYFTFR
                     ITDEAGNIVSLKAEVWNIDEERPRIALKGNLYECIMLDEPYAEAGYKATDNIDGDITG
                     KVTVEGSVNTAEAGTYLLKYKVSDAVGNSTEEIRTVRVLESGELVLLLNGKVAEGPMM
                     LLDAKNIKANTLGNEGDFVIKWGEGRRTIAWFKGNGNILAEGTNVWLETGRWYTFFIQ
                     DRERRTKSIQVYINE"
     gene            complement(23657..27034)
                     /locus_tag="PKI50_01985"
     CDS             complement(23657..27034)
                     /locus_tag="PKI50_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT02518.1"
                     /translation="MKKSIGLILGIVVLLAVTLGIVAVQADSVIIPDGDYTIVNNGVY
                     QLSSGYSGVITVSNAVYEVTVTDSVYKTPHEETRIYIAEGRTEALELTIEDLDMSASL
                     SSVIDLRNAGSYDNKLYISGICNLTGENSAPLHVPEGVQLTVDKKESTDNDEYAQLIV
                     VNNGWHSAGIGGDASEETGTINIMGGTIKATGGMYSAGIGGGFGGSGGNITISGGVVE
                     GIGGIQGAGIGGGVGATGGNITITGGTVTAISRFGGAGIGGGTDFEDEVNDGGNITIS
                     GGHVRAISGGYGAGIGGGNEGSGGNITINGGYVEASCEVYGDNVGQGAYGAGIGGGSN
                     RNHPHYEDDLDNNGGIITINGGTVIARGGSTAAGIGGGEFGEGGTVVINGGTVEAYGG
                     NNGAGIGGGSNRDGGTLTISGGTVKATGGFNAAGIGGGSNGTGADVTILNTPTVIAID
                     VEPGWNEYPAKPIGNGGGFQELDMGTLRDGAGNNLSYLLFDTSPAVGVNIRLEGAEDL
                     DGEYLTDEEGLYWIFVPDRSGASYSYIMNKAGYSAVEGSGLFTTSSGISVEMTLDDTK
                     PEISQVTPMVIKAGGNVTVACSDYGIYGSVYLAAKRSTAYTSKAQLEAVYIEKLEAAS
                     SVSIESTGLTEGYYQIYLIDSAENVSVPEDIFVDTTEPVAASRDITLSDITYRSIRLT
                     WDEAADNFTDAANLEYIIYKSYTNNLDSVESIETNGTVAVNENITGSTGYTVAGLNDE
                     TTYYLNIIVRDQAGNKICYNSVSAKTLKAPSDNVRSRTPAPTAAWKIDIPVTDLIGAS
                     GSMKEVKNGPATLIIPSNMLTAEMLGTAQTIQLVIGKADTSGLTAELQAQIGSRPVIE
                     LELKLDGRTIAWSNPDAPVTVSIPYVPTAEELKNPENITVWYIDGAGNAVSVPSGRYN
                     PATGKVTFTTAHFSRYAVVYVIKTFNDLYSYDWANKQIEILAAKGIMTGRREKSFEPA
                     AGITRAEYIGALVRTLGVTAKAESNFKDVGKDNVYYAEIAIAKKLGITNGTGNNSFKP
                     EDIISRQDMLVLTERALRNLKKISSTGTSKDLEKFADAREVAGYAVESIAGLVMEGLV
                     QGSSGKLNVGAGTTRAEAAVFLYRIYNIINYTEFLDN"
     gene            complement(27163..27807)
                     /locus_tag="PKI50_01990"
     CDS             complement(27163..27807)
                     /locus_tag="PKI50_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT02519.1"
                     /translation="MKMKLLLVDDHQLFMEGLQYLLETYGVEVVGTAEDGREAFEKAR
                     VLKPDTILMDIKMPGLSGLDALKLIKAEMPDIKVVMLTTSEEDDDLFYAVKCGASGYL
                     LKNTNAKELVGILSDIEKGEATFSPELAAKLLKEFRSRDTGKTIQNEELTKRQLEVLE
                     LVSKGIIYKEVGEKLGLSERTVKYHMGKILEELHLENRAQVIAYAARIGLVDKN"
     gene            complement(27782..29524)
                     /locus_tag="PKI50_01995"
     CDS             complement(27782..29524)
                     /locus_tag="PKI50_01995"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNT02520.1"
                     /translation="MRIQYIPYIWPLITSAFISLSLGIFVILRRRDSKGAVSFILSML
                     VVTIWSLGNALEMASIDFSTKLFWANVQYFAYCYSPLTLLAMCMEFTGYDRWIRNRKV
                     LWLAVIPTIIVILVWTDHLHGLIRYNMHMNYSGSFPVIDKKYGPAFYIHALYSHMLNI
                     LAWILLIKAVFFRNTVYKKQATALLVGLSMIVIPNVLYITGLSPIERYDITPLFFGPA
                     GLIITWGIFRYRLFDVIPVARAMVIENMDAGVIVLDLQDRVLDINPAMRRIIGYPDVK
                     TPAETVEKVCRKIPELACTCKDRNITHSEFVIGINEGMKVYEAFFSPLADQKGAMIGR
                     LVVAYDITEKKQEQERYLKQQWKNAVTEERERLARDLHDNLGQVLGFINLQAQGIEQE
                     LINADIDIVSHKLDKLVRASQEAHNDLREYIRNARNAEALEKDFMTGLTRNIASFEEQ
                     SGLNVKVNIPLGFTGDELRPSIRINILNIVKEVINNIRKHSEANNVKFEFAIEQRQLC
                     VTVEDDGKGFDVENCLNNNNTTRFGLNIIRERASEIGAQIGIQSEPGRGCRISLIIPL
                     NKEELQNENEITAG"
     gene            complement(29537..30325)
                     /locus_tag="PKI50_02000"
     CDS             complement(29537..30325)
                     /locus_tag="PKI50_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HNT02521.1"
                     /translation="MENSKDSLNRKVASALKEAERTCIPIEPISKTYPELKIKDAYEV
                     QRINVNELLASGNKITGKKIGLTSLAMQQFIGVDQPDYGQLFDFMEVKNGILERSRYL
                     NPKVEGEIAFVLKKDLQGPNVTAKDVMEATDYVLASIEVVDSRIKDWKIGIVDTVADN
                     ASSAMYVIGNKKVDPNSINLKAVSMGLYKNGEKVLSGVGADVLGDPALSVAWLINKLW
                     EYGVTLKKGEVVLSGSLTSALAAEAGDDFKVVFSELGEVSLRFV"
     gene            complement(30340..31113)
                     /locus_tag="PKI50_02005"
     CDS             complement(30340..31113)
                     /locus_tag="PKI50_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNT02522.1"
                     /translation="MKNEVKSKMESGLPVIGTFFELGDAAAVECIGLSGMDFLIIDTE
                     HGPFDVESAMDFIRAAELRGIEPFVRIKDHTRSSVLKMLDVGAKGLIIPNIETVEQVK
                     KLVEYGKYYPVGRRGFAPTRCGGYGFVEHAAGNLEEYFSLANNETMIIPQCETKGCLD
                     NIEEIVSIEGVDGIFVGPYDLSIALGMPAQFSNPVFTESVDRILNACKAAGKSIFIFS
                     GSVEAARKYIKDGFNGIAYSLDTNILVSALKDCVRQIKG"
     gene            complement(31219..32268)
                     /locus_tag="PKI50_02010"
     CDS             complement(31219..32268)
                     /locus_tag="PKI50_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HNT02523.1"
                     /translation="MFIAITIIIGIIGGLTAVRLKMPAGAIIGSLISVALFSIFTGKA
                     EFPQSFKLVTQIGTGAYIGARICNKDVAEIKSIIKPAIILTVSMCLFGIFAGTLLSII
                     SDIDLTTALFATAPAGIADMTLISVDFGADSSKVAAVQLVRLVSVIAIMPNLIKYYLK
                     KSNKAQIEDKPPEKYEESCADYNEIFKRTLITMTVGFVFGFIGHVTGFPAGSISFSMI
                     ACAVYNIRTSRAYMPINLRKGIQFLGGALIGARITMEQVMGMRGMLPALIIIIAGYIL
                     LTFVSGFLLIKFSTLDVTTAMYSSTAGGLTDMAIIAGEMGADAPKVAVLQFARVVSII
                     AFYPVVIKLLLHILT"
     gene            complement(32472..34352)
                     /gene="htpG"
                     /locus_tag="PKI50_02015"
     CDS             complement(32472..34352)
                     /gene="htpG"
                     /locus_tag="PKI50_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNT02524.1"
                     /translation="MAKKQFKTESKKLLDLMINSIYTHREIFLRELISNASDAIDKIY
                     YKALTDESISFDKDSYYIKVVPDKENRTLRISDTGIGMTKEELDDNLGVIAKSGSLSF
                     KKEIELKDGHDIIGQFGVGFYSAFMVSDTVTVISKAYGSDDAYKWQSSGADGYTIELC
                     EKDTVGTDIILKIKENTEDEKYDEYLEEYRLKAIIKKYSDFIRYPIKMDMVSKRLKEG
                     SDKEYEGYKEEQIINSMVPIWKRNKNELTKEDYDNFYSEKHYGFDKPLKHIHISVDGA
                     VSYNAILFIPEKMPFDFYTKEYEKGLELYSKGVMIMNKCPDLLPDYFSFVKGLADSED
                     LSLNISREILQHDRQLKLIAKNIKNKIKSELENLLKTERDKYEEFYKAFGRQLKYGIY
                     SEFGSNKETLQDLLMFYSSKEKKMVTLDEYVSRMSEEQRYIYYATGESNERIEKLPQA
                     ELVSDKGYEILYFTDDVDEFSIKMLMTYKDKEFRSVSGSDLGIEAEEKKNLSESEEKD
                     SKELFEHMKSILSGKVKDVRASVRLKNHPVCLSNEGELSIEMEKVLNSMPNSPGIKAD
                     KILEINTDHEVFKSLKEAFEKDKEKLNLYTNLLYNQALLIEGLPIGDPVEFTNNICKI
                     MV"
     gene            complement(34590..35609)
                     /locus_tag="PKI50_02020"
     CDS             complement(34590..35609)
                     /locus_tag="PKI50_02020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNT02525.1"
                     /translation="MKIITFTVPCYNSEAYMKNCIESLLLGGEDVEIIIVNDGSTDGT
                     GEIADDYASRYPGIVKAIHQENGGHGEGVNTGLRNAAGLYYKVVDSDDWLDADGMLKV
                     LSKLKELHEKGTALDMMVCNYVYEHSSDNSSHVVNYKYVLPQNRVFGWDEIGNFKPTQ
                     YLLMHSVIYRTELLRQCGLVLPKHTFYVDNLFVYNPLPYVRTIYYMNIDLYRYFIGRE
                     DQSVNEKNMIKRIDQQIRVNKLMIDAYNSYDYLPSRRLANYMRSYLSMMMTISSVFLQ
                     ISATKEDLQKKAELWSYLKKSNEMLYYRLRYRFLGTLSNPYGKVGRKITIQLYRLMRR
                     IYKFN"
     gene            complement(35630..36784)
                     /gene="glf"
                     /locus_tag="PKI50_02025"
     CDS             complement(35630..36784)
                     /gene="glf"
                     /locus_tag="PKI50_02025"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HNT02526.1"
                     /translation="MKNEFDCLVIGCGFAGAVIAREHAERGGKKVLIIDKRSHIGGNA
                     YDSYNDDGILIHNYGPHIFHTQSSRVFEYLSRFTEWRDYSHEVLADIHGKLVPVPFNL
                     NSLYLVFEQSKAERLEQKLISVYGTEKRVTISELRGQTDSELLELADFIYKNVFMYYT
                     QKQWGIAPENIDPSVTARVPILISYDNRYFQDEYQGIPLNGYTSLFERLIDHPNISLQ
                     LNTDSASVLKLVENRIYFCGSPFYGRVIYTGAIDELFDCRFGRLPYRTLDFLFETHEK
                     TWYQSKGTVNYTVDEPYTRITEFKHLTGQIIPDKTTIMKEYPREYTGAGKEIPYYAII
                     NPENEALYGNYKQITEAIDDFYLLGRLAEYKYYNMDVIAERALSLADSII"
     gene            complement(36852..>37505)
                     /locus_tag="PKI50_02030"
     CDS             complement(36852..>37505)
                     /locus_tag="PKI50_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT02527.1"
                     /translation="MGEWIVMKILALLKNSKGFYDNQRQKKWQPDSTLLELYNKTVCF
                     IGTGSIALEAAKRLQGFDVNILGINSTGRKVEYFNSCHPMNELHNILPQADVVVLSIP
                     YTKETHHLINEESISFIKKGACLVNIARGSIIKEPALIDALRKGNIHGAALDVFEKEP
                     LPEESPLWEMNNVIITPHNSWISEMRTERRYNIVYENLRRYINGEALLNIVDIDKGY"
     assembly_gap    37506..37605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(37872..38276)
                     /locus_tag="PKI50_02035"
     CDS             complement(37872..38276)
                     /locus_tag="PKI50_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNT02528.1"
                     /translation="MKKLSMIEGIGEAYNEKLKDAGINSVEELLEACLTKKSRSELAE
                     KTGISEKLILKWTNHADLFRIKGIGGQYSELLEAAGVDTVPELANRKPDNLLAKMESI
                     NQEKKLIKAMPFKKQIVKWIEQAGNLPRKIQY"
     gene            complement(38325..38897)
                     /locus_tag="PKI50_02040"
     CDS             complement(38325..38897)
                     /locus_tag="PKI50_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNT02529.1"
                     /translation="MDIVKLLTNQLNSPDTLKKLAGSVGVESSQAQKLTQLGIPAILQ
                     ALGRNASTKEGAEALLGALDGHQKDEIEDLTGFLNKVDTNDGAKILQHIFSGNNKRVQ
                     ENLARQTGLNTSQVSGIMTQLAPLMMGALGKKKKEENLDVSGVVGLLGGLMDGGDNNL
                     MGIVTNLLDSDNDGDVMDNIGDLLGGLFKK"
     gene            complement(39152..40342)
                     /locus_tag="PKI50_02045"
     CDS             complement(39152..40342)
                     /locus_tag="PKI50_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT02530.1"
                     /translation="MDRRIVIAGGGIAGLNAAKSAREQDPDCSIVILEALNTNTYVRT
                     RLPDYISGTASYKEIFPYDDSWYEKNRINLKKDTRITGIDAAHKTIITDKGNYDFDSL
                     VIALGSSGNIPPIPGAKLENCFPVRTVADADKIRSLSCGGKICTIIGGGLLGLEMAWA
                     IRQLGCDVNVIHNSGRLLPKQIDEEGAKLLYKAISNKGINLYMNAQVQEIGGENRAEY
                     VRLDSGTVVKSDFVILSVGVNANTQPLKDSGINMGRSVAVDKYMETNIEGIYAAGDVA
                     EYNGKCYGIWPIAVEQGKIAGTNAAGGKLEYSEIHPFTSLKIKGITMFSIGDVFSEDS
                     LSIAEADFESGRYIKLLIKDSIIIGAIVFGDSNLPMKIKKAVDNRIRVPEIKEGITIK
                     ELIL"
     gene            complement(40388..41788)
                     /locus_tag="PKI50_02050"
     CDS             complement(40388..41788)
                     /locus_tag="PKI50_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT02531.1"
                     /translation="MNEAENDKRERKNFLNIKGKADAEVMRIAWPVLAELLLGSLFGM
                     VDMMMLGRISDNALAAASVAAVGMTNQPLFIGISLVQALNIGGTAMIARYFGANQNEK
                     IEITLKHVMLLSMLMLAIPLSILGFVFTDSIMKLMGAQADTLQVGRIYFRIIMVGYVF
                     QSLNMSISAALRGIGETRVPMIINLRCNFLNVIGNAILIYGLLGFPRLGITGAGISTA
                     LSNAVASVFIFGYVLKGKSVVRFSIKKPFNLNRRILYNLFKIGMPASFEQLVLRTGVL
                     LFVRIVAELGTVIYAAHQIALSILGLSFQPGQAFGIAASSLVGISLGAGKPDMAEEYA
                     RKTRKMGSIISTFMAIIFFFFGPQLVGLYSNDPMIIKNSSVALKIIALVQPFQSSQFI
                     LAGALRGAGDTFWPLVSTSVGVLMVRVVLAYIFVSVMGYGLMGAWIGVLVDQLVRWMF
                     VYFRFKTGKWKYIKIR"
     gene            complement(41912..43069)
                     /locus_tag="PKI50_02055"
     CDS             complement(41912..43069)
                     /locus_tag="PKI50_02055"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT02532.1"
                     /translation="MINPFEYVLPTKIRYGAGMLKVLGEELRLLKAKKVMIITDKGLV
                     NAGMIKKAAEIIEAEDIDFIVYDEIEANPKDYNVEACAEKARAESVDTLLAFGGGSPI
                     DAAKATAVLARQGGKVRDYQGKGRIKDDCLPLITIPTTAGTGSEVTFSSVITDTKEKF
                     KFTIKSTAIAAKTAIIDPELTLSVPPLITAATGIDALTHAIEGYTANCTEPIAEAAGL
                     YAVEYIAGNIEEAVKNGRNIAARDKMMIGSLLAGLSFSHADVASVHCMAEALGSMYDA
                     PHGMCNAILLPYVMEYNLPAAGHKYARVARAMGIEEKDDYTAAVKGIEHIRKLSKEIG
                     LPGIRSLNVNTDDFELLAEMSEKNGSNDSNPRKISKDEYVKLFYKAYNDCN"
     gene            complement(43099..44499)
                     /locus_tag="PKI50_02060"
     CDS             complement(43099..44499)
                     /locus_tag="PKI50_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT02533.1"
                     /translation="MGGLFGVVSREDCVMDVYFGTDYHSHLGTCRGGMAIWGGSCFNR
                     SIHNIESTQFRAKFEAELSKKSGNMGIGCISDTDPQPLTVRSHLGQYAISTVGRINNL
                     DEIAEKAFSNRNIHFLETNKGIISPTELVSVIIDHEDSFKEGLLRAQELIQGSCSVLL
                     LTPKGIYASRDRLGRTPLMVGSKEGAYCISSESCTFANLGYKSDYELGPGEIVFLTPE
                     VVEQVSPPGDKMKICAFLWVYYGYPSSTYEGMNVEVMRYRNGAAMAKNEDLGIDIVAG
                     IPDSGLGHAIGYSNQSKVPYGRPFIKYTPTWSRSFMPQDQDIRNLVARMKLMPIPEIV
                     ADKRLLFCDDSIVRGTQLRETVDLLYKCSAKEVHIRSACPPIVFGCKYLNFSASRSEM
                     DLAARQAIKELEGKEPDSLEEYCNQATDKYHNMVDLICRKLNFSSLKYQNIHDMIEAI
                     GISKDKVCTYCWNGKE"
     gene            complement(44683..45618)
                     /locus_tag="PKI50_02065"
     CDS             complement(44683..45618)
                     /locus_tag="PKI50_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02534.1"
                     /translation="MEAVISFLHAAIVAGTPLLFATLGEIITERSGNLNLGVEGMMLM
                     GAVIGFMVGLNTESTTLALLAAMIAGAGGALIFAFLTITLRANQVVSGLTLTIFGTGF
                     ADFVGQDLIGQIAPESLRLFFRPYTIPLLGDIPFIGPVLFRQDAFVYLGYAASILLGL
                     YLYKTSTGMNLRAVGENPGAADAAGINVTLYKYLHTLLGGALCGLGGAYLSLVYVPAL
                     QEGVTAGRGWIAVALVIFATWNPYKALFGAYLFGGLDIIGFRIQNLGIDVSQYFIDML
                     PYLVTITVLVVVSMRKSREKAGPKACGIPYFREER"
     gene            complement(45621..46715)
                     /locus_tag="PKI50_02070"
     CDS             complement(45621..46715)
                     /locus_tag="PKI50_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02535.1"
                     /translation="MIMPITIRAIKKSEPGRMKAASIRIGAIILALVASSLFILLLSL
                     NPADVYIKMIQGAFGTAYRIKETIVKAIPLVITSLGIAIAFKMQFWNIGGEGQIAIGS
                     FAASYFALYMNDMPMPLMLTVMILAGMLAGGLWALIPAFFKAQWGTNETIVTLMMNYI
                     ALKWVMFLQYGPWRDPKSLGFPKIANFPQNAVLPKLLGVHVGWVIALILVVVVYIFIN
                     HTKKGYEIAVIGESMNTARYAGINIKKTILIAIMLSGGLCGLVGMIQASAVNYTLSVE
                     ITGGVGYTAIITAWLAALSAPLILIVSILFAALLEGGSYIQTVFGIPQAAAEILQGII
                     LFFVLGSEFFVRYKFVIVKKHSAIAEKGAD"
     gene            complement(46684..48243)
                     /locus_tag="PKI50_02075"
     CDS             complement(46684..48243)
                     /locus_tag="PKI50_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02536.1"
                     /translation="MDNNYISMKNINKSFGKVVANRNINLAVRRGEIHALLGENGSGK
                     STLMNMLSGVYTPDSGSIFIKGKEVQFAAPKDAIEAGIGMIYQHFKLIDVMTAKENII
                     LGRKNSFLMNEKTLSDKIRDMSARFGLDIDPDKKVYNMSVGEKQTLEILKVLYRGAEI
                     LILDEPTAVLTPQETEKLFKIMRKMKEEGCAVIFITHKLNEVMEVADRITVLRKGETV
                     MTVDKADTNPKHLTELMMGCPADLSIKRIETEKNNVAMEVRNLSAVNSEGIKVLKGMS
                     FDIYSGEILGVAGIAGSGQKELCEAIAGLHPVKQGEIVFNGENLVGKSPAEIITKGVS
                     LSFIPEDRLGMGLVASMDIVNNVVLKLYQKQKGIFIDRKPAEQKSVEIIERLSIQTPG
                     IKHPVRMLSGGNIQKVLLGRELDSRPQVLITAYPVRGLDVNSCYTIYDLLNEEKRKGV
                     AILYIGEDLDVLLELCDRIMVICAGEITGIVDARYTTKEQIGLMMTGTVNLMKEGEKS
                     EHDNANNYTSH"
     gene            complement(48263..49393)
                     /locus_tag="PKI50_02080"
     CDS             complement(48263..49393)
                     /locus_tag="PKI50_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT02537.1"
                     /translation="MKRTISILLVLVLVFAAMLTGCAPKAEPAPAAPETPAPAEEPKA
                     EPLKVGFVYIGSAKDGGYTQAHDEGRLYLEQQLGDKVITMYKEDVPESSECEKVINDL
                     VDQGCTVIFTNSFGHMDYTEKAAQAHPDIKLYHCSGYKSGPNFSNYFGAMEEARYLAG
                     IVAGLRTQNNTLGYVAAFEIPEVVRGINAFTLGAQSVNPKVKVKVNWTHTWYDPAKEK
                     EAAKALLDAGCDVLAQHQDTTATQQAAEERGVWAIGYDLDTRDSAPKAYLTAPVWNWG
                     KFYAAEVQRILDGKWKQENYYGNMKDGLVDLAPLTDNVAPGTQEAVDKVKAKIIDGSF
                     YVFQGPIKDQTGAVRVPEGKSMTFDEIMSVDWFVQGVEGKIK"
     gene            complement(49481..50050)
                     /locus_tag="PKI50_02085"
     CDS             complement(49481..50050)
                     /locus_tag="PKI50_02085"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNT02538.1"
                     /translation="MELLKDRILKDGKVIGDSIIKVDSFLNHQLDIELFNEIGREFKE
                     RFSDEKISRILTVEASGIAVACIAAQYFKVPVVFAKKYEALNLDSDTYQSEVYSFTKQ
                     RNYNIRVSKKYITPEDNVLIIDDFLANGKAASGLVDIVLQAGARVAGIGIVIEKGFQK
                     GRETLRQKGVRIESLVIIKSMDEGVIIFD"
     gene            complement(50179..50628)
                     /locus_tag="PKI50_02090"
     CDS             complement(50179..50628)
                     /locus_tag="PKI50_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT02539.1"
                     /translation="MVVNGKAYSLEVDIRESLLEVLRNRLHLTGVKQGCSVGECGACT
                     VLIDGFPVNSCIYLAVWADGRKITTIEGVSKEGELSKVQQAFVDEGAVQCGFCTPGLV
                     LTATALTESGRKYSDEEIKREISGHLCRCTGYNKIFNAVKRALDVDC"
     gene            complement(50645..51526)
                     /gene="xdhB"
                     /locus_tag="PKI50_02095"
     CDS             complement(50645..51526)
                     /gene="xdhB"
                     /locus_tag="PKI50_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HNT02540.1"
                     /translation="MFDIVNIEKPGTLEAALEMLSRNPELKIIAGGTDVLIKMRNGAL
                     EEAELLSLRNIPGMDEIRILDNGTISIGAMAAFAKIARSSIINENLPVLSEAAVSVGG
                     PQIRNMATIGGNICNGAVSADSAATLLAYNAELKLLSRSGSRTVPVSEFYAGPGKVNL
                     LPAEILTDILIPEAGYGRTSGCYIKYSGRKAMDIAMLGVSVVCKTEGGKFSDVRIALG
                     VAAPVPIRCSEAEAYAIGKEISDKTMKETGRYAVKASRARDSWRASKAYREHLIEVLV
                     FRALKEAVIRGGGVDIG"
     gene            complement(51542..53833)
                     /gene="xdhA"
                     /locus_tag="PKI50_02100"
     CDS             complement(51542..53833)
                     /gene="xdhA"
                     /locus_tag="PKI50_02100"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HNT02541.1"
                     /translation="MAFEIVGKRTIRKDAFAKVTGRAKYADDFFEGDMLVGKVLRSPY
                     AHAIVKRINTSRAKALPGVEAVFTYEDLPKLKYATAGHPWSLEPDHRDAADRLILTDK
                     ARYVGDGIAAVVASDAFTAEKALKLIEVEYEVLPFVLDPEKAMLPGAPVIHEEKPDNI
                     LGDASEEIGDIEEDFGKADHIFEGVYETSIVQHCHLEIQSAYAYADADGRIVIVSSTQ
                     IPHIARRIVAQALGMPWGKVRIIKPYIGGGFGNKQDIIVEPIAAAMSLAVNGRPVRYA
                     LTREECFVDTRTRHAMKFYMKTAVTKEGKLLSLHMRPISNNGAYASHGHTIAMSAGGK
                     SRLLYDFNSMKYEPKTVYTNLPAAGAMRGYGCPQIFFALESHIEDIAREMKMDPIEFR
                     KRNLISAGKTDPHSEIVVRTFGLPECIEKGKQLIKWDEKLEKYKEQKGTKRRGLGMAC
                     FSYASGTYPQGFEISGARIVMNQDGSVQLQVGATEIGQGSDTVLSQIAAEVLGLPYDM
                     VHIVSQQDTDISPFDLGAYASRQTFVSGAAVKKAAEEVKSKVLEMASEKTGLSIDELD
                     IKAARIVERKSGNEICTLEDIAMGSYYDRLNAEPICGNASVNVRTNAMAYGVTFAEVE
                     ADMETGRIEVIEIYNVHDSGVIVNPMLAEGQVHGGVSMSLGYALSEQLLFDPETGRPL
                     NNNFLDYKLQTIMDTPDIGSAFVQLHEPEGSVGQKSLGENTTISPAPAVRNAVLNATG
                     IAFNKLPMTPQKVFEKFKQSGLL"
     gene            complement(53868..55196)
                     /gene="ssnA"
                     /locus_tag="PKI50_02105"
     CDS             complement(53868..55196)
                     /gene="ssnA"
                     /locus_tag="PKI50_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNT02542.1"
                     /translation="MILVGNGRVITRDDDIPIIENGCIAIDGNKIAELGLTSELKEKY
                     SGARFIDAKGKLIMPGFINTHMHYYSTFARGMSNDSPPATKFSEILKGLWWRLDKVLT
                     LEDVYYSAVGPMIEQVKCGVTTVIDHHASPYAVKGSLFKIAEAAEHIGIRSNLCYETS
                     DRDGEKIAEEGIAENVEFIKYCSNKKDDMLKGMFGLHASMTISEKTLDKCLDAISGLD
                     TGFHVHCAEGIEDLEDSRARYGKGVIERWYDSGVLGSKTIAVHCIHISEKEMDMLKEK
                     DTIVVHNPESNMGNAVGASPVPEMFGKGILLGLGTDGYTADMMESYKVGNIIHKHVKG
                     DSNVAWGEIPAMLFANNRKIVERFIDGQVGILKAGAMADVIIVDYNPPTPINEGNING
                     HLLFGINGRFVDTTIVNGRILMEDRKLVNIDEERMMARSRELAREVWKRF"
     gene            complement(55213..56589)
                     /locus_tag="PKI50_02110"
     CDS             complement(55213..56589)
                     /locus_tag="PKI50_02110"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNT02543.1"
                     /translation="MSQKLLKNIDLIVTFNDREETFEHCDILIDGTAIREIGSGLDTA
                     GDAEVIDCSGLTALPGFVNTHHHLYQTLFRGIKEVQEKPLFPWLVGLYEFWKNITPEA
                     VYYGALVGFAELLRTGCTLTSDHHYVFPKNQPGTLIDDQIRAAREIGIRFHATRGSMA
                     LGRDMGGLPPMEVVQAEEAILADSERLINKYHDSSPYSMQRIALAPCSPFSVTRELMV
                     QTKELAREKGVMLHTHLAETLDEEKFCLERYGRRPFELMEDLEWVGDDVWFAHGIYLN
                     DGEIKRLQGSGIAHCPSSNMKLSSGICRTTEIIRAGGKLSIAVDGSASNDGSNMWEEV
                     RRAYLLNHLKYGTDGLTAYQVLRTATRGGAEVLGRDDTGCLDIGKAADIVLFNLKGIE
                     YSGCHDPLVSLICLGNSSMVDMTVVNGRVVVKDGKLTTIDSDRVASDAHKTAVEIVKR
                     QRNSIRNH"
     gene            complement(56911..59928)
                     /gene="ygfK"
                     /locus_tag="PKI50_02115"
     CDS             complement(56911..59928)
                     /gene="ygfK"
                     /locus_tag="PKI50_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HNT02544.1"
                     /translation="MSDRMRLIPFEKMLSWVLSELKEKDSIFGIHKSKFYKNKSGSFT
                     ELFGEKLATPLGPAAGPNSQLAQNIVAAYLTGCRFIELKTVQKLDGEDLPVSKPCIYA
                     QDECYNVEWSTELRVPDAYNEYIKAWFLLHVLMKELGLSEKRDFMFNMSVGYDLEGIK
                     SPKMDAYIEGMRNAANTDIWKECRKVLLSAMNQFTHFTREDLDMISPVVCPSITLSTL
                     HGCPPQEIERIANYFLKEKKLHTFVKMNPTLLGEKFVRDTLDAMGYGYITLNGHHFIH
                     DLQFPDAVAMLKRLKALARELNLEIGVKLTNTLPVKILNQELPGEEMYMSGRSLYALS
                     ISLASKLAKEFNGDLHISYSGGIDFFNIDKVMEAGVRPITFATTILKPGGYERINQLG
                     HKAEGLQKRMAAGIDIAKISSLAENALTDKHHLKELRPVNSRKLTSELPLYDCAVSPC
                     AMGCPINQQIPQYVALVGEGKYDEAFEVIVSDNASPAITGSICDHQCQYKCTRLDYDE
                     SVLIREMKKTAALKAQDKYTAGIRPAEIRSNKKAAIVGAGAAGLATALFLRRNGMEAV
                     VMDKRERPYGVVEYVIPEFRISSDMVRKDLELVKRSGVGFKLGINENFSVEALKKEYD
                     YVVLAIGACKPGQISLEEGAEKTVNAISFLENYKAQKGNMKLGSHVCVIGGGNVAMDA
                     ARAAKRVPGVESVSIVYRRTKALMPADREEIHLAASEGVLFRELLSPAAYKEGKLVCE
                     VMALGDRDSSGRRSPVPTGEKITLDADTVIVAVGEQVDSIFLAKNGIELDSKGFPKVS
                     SACETNIPGVYVAGDAKAGPATIVRAIADGKAAAKDILSKEGLKADFEKKVLPVDEKI
                     LYERKGILNNPVYSDKEAERCLSCENICELCVDVCPNRANAVIFVDEGFASGHQVIHL
                     DGMCNECGNCGIFCPHEGNPYKDKVTVFWNEEDFRDSNNKGFLVTDMAACKCLVRTEE
                     GQIVNYALGQENGVSKEMAAIIKACIGKYSFML"
     gene            complement(59978..61222)
                     /locus_tag="PKI50_02120"
     CDS             complement(59978..61222)
                     /locus_tag="PKI50_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNT02545.1"
                     /translation="MSYDFKQILELAEKYKPQMSKFLRDMIAIPSESCGEKEVIGRIA
                     EEMRAVGFDKVELDPMGNIMGYVGHGKHLIAMDAHIDTVGVGNIKNWEFDPYKGYEND
                     EIIFGRGASDQEGGMAAMVYAGKIIKDLKLEDDFTLLITGTVQEEDCDGLCWQYIIKE
                     MNIRPEFVVLTEPTSCRIYRGHRGRMEIKVATQGVSCHGSAPERGDNAIYKMAPILME
                     LKELNERLISHPFLGKGTLTVSEIFHTSPSRCAVADSCWISVDRRLTAGESGEYALNQ
                     IRELPSVKAAKAEVTMYPYSRPSYTGLEYPTEAYFPTWFIEEDHPVCSTLLEAYKGLF
                     EDEPVLDKWTFSTNGVSIMGKYGIPCIGFGPGHEDQAHAPNEKTWKDELVKAAAMYAA
                     IPSLYVKKYADKMPKTTENLAD"
     gene            complement(61234..62715)
                     /locus_tag="PKI50_02125"
     CDS             complement(61234..62715)
                     /locus_tag="PKI50_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNT02546.1"
                     /translation="MRKLIDFTINEKGLEKAVQNVKERNIVIPTFAQMKDPDKIPAKV
                     KEKLKNTGLWDVDPINLFRISWKNEPVKHGGLYNKLPNYIEIPREISGVKARIFAMAG
                     KFFPTGAHKVGASFACLVPRLVTGQFDPTYHQAVWPSTGNYCRGGAYNSTLLGCSSIA
                     ILPENMSKERFEWLKTVAGEVIATPGCESNVKEIYDKTWDLRRTRDNVVILNQFGELG
                     NHLWHYEVTGHAMEDIINAEAGSKGKVAGVCLTSGSAGTLGAGDFIKDRYPNAKLAVG
                     EALQCPTLLSNGFGDHRIEGIGDKHVPWIHNVKNTDMVIAIDDNDSLGLFRLFNEPAG
                     QEYLKSLGIRDEVVEKLSWVGISGAANIISCIKFAKYYELAENDIVMTVLTDSAEMYQ
                     SRLMEMEEARGRKYSNVDAAIDHNRYVLALGTDTMEELTYQSRKRIHNLKYYTWIEQQ
                     MYDLDELNEQWYDYDNYWGRLHKMAPELDSLIEEFNKRTGLGY"
     gene            complement(62769..64004)
                     /locus_tag="PKI50_02130"
     CDS             complement(62769..64004)
                     /locus_tag="PKI50_02130"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT02547.1"
                     /translation="MSKVLKLKCVKCGREYNEAEILYTCRDCGIDGILDVELDYDSIR
                     KELTREYLKNNKDYSLWRYLPAIPLEKTGGIQPLQVGWTPLYNIKSLSKETGLSSFFL
                     KDDSRNPTASLKDRASAVGVAKAVELGRNVMCAASTGNAASSLSGFAAVAGIKTYIFV
                     PETAPEAKVTQLLIYGSNVFLVKGTYDEAVELCFKAAEEFGWYNRSCAINPYLVEGKK
                     TISYEICEQLDWKAPDIAVVAVGDGCTIGGVWKGFKECFELGLIDKFPKIVGVQAENS
                     NPVTRAFNKKTYEFEYRVPDTLADSISVGIPRNGIKALNALHESGGYMVDVSDEEILA
                     AMKILAQKTGVFGEPAGVTSFAGIMKMKELGLLKGDEKVVSIVSGSGLKDIKSAVKAA
                     GKANHVEPDLNDLKRIVHI"
     gene            complement(64001..66571)
                     /gene="xdh"
                     /locus_tag="PKI50_02135"
     CDS             complement(64001..66571)
                     /gene="xdh"
                     /locus_tag="PKI50_02135"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HNT02548.1"
                     /translation="MHLISFKVNGRAVNVREGTNLLKYLREEERLTSVKNGCDSGVCG
                     SCTVLIDGKAARSCTLKADKLQGREVLTVEGLDGKEKEVYSNAFAEAGAVQCGFCTPG
                     MMLASKALLDKNPDPSEEEIKKAIRNNICRCTGYVKIVKAIRLAAEALRESKHISLST
                     CSSNIGQNWRRVDAIQKALGEATYCDDVFMEDMLYVSVLRAMKPRARVLSVDTDAAKA
                     LEGVKAVLTAEDIPGENYQGYIFKDWPTFVPVGEMTRYAGDAVAAVAAETKELAEAAI
                     RLINVTYEEMEPVTDAKYAMRSDAPKIHEKGNILSRTYVKRGNPEKAMAESKYVVKNT
                     YRTPPVDHAFIEPESAAAFYEVDVLTVYAATQSITHDHHELCRVLGLPHDKVRVILQN
                     VGGGFGGKEDLSAQHYAALAAFYTKKPCKYTFTREESLLCHPKRHGMELEVATGCDER
                     GMLTFMTARIIADTGAYASLGTAVLERACTHVCGPYSIPNVELEGFCVYTNNAPAGAF
                     RGFGVPQSNFAGEANMDQLADLAGIDRWEFRYRNAVEPGMVLANGQVCGDDTAVKETL
                     LAVRDEYYRSRYAGIACAFKNTGIGVGLPDVGRCRIRIKEGKVVVYSAASCIGQGLAT
                     VMIQIVSETLGIDGSDIIFSQPDSLLCPDSGATTASRQTLFTGEACRRAALKLKAALA
                     EKTPEELEGVVFEGEYSGVTDPINSSRENPVSHVAYSYATHLAILDAEGRIAKIVAAH
                     DIGRAINPLNIEGQIEGGVVMGLGSALRERLIYEGGILKSRFGTLGLLRADEIPEIDV
                     KIIEKNQSELAYGAKGIGEISLIPVAAAVASAYQRFDGIRRFELPLEGTPYDRKKVEE
                     "
     gene            complement(66589..67167)
                     /locus_tag="PKI50_02140"
     CDS             complement(66589..67167)
                     /locus_tag="PKI50_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT02549.1"
                     /translation="MSNEVTNKILDAALEIVAQNKISGTRMHLIAKEAGVVQSNVHYY
                     YPTKNDLMIALLDRIQERFTEKRMSSVDLEKKSFEENLRGLFHEKKDDIYNHKKIDYV
                     QFDFWVQGTANPEIREKFIGAFNIWRSNITEVLNQMPGFPECKSEQCAMLPYLLVSIM
                     MGASMQYLIDEGKFNLDAYFDAAENMILNFVK"
     gene            complement(67219..68415)
                     /gene="ygeW"
                     /locus_tag="PKI50_02145"
     CDS             complement(67219..68415)
                     /gene="ygeW"
                     /locus_tag="PKI50_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNT02550.1"
                     /translation="MEEIRKLVSQFKKLDSNAMCNNDFLLTWEKTDDEIHAVFQAAEI
                     LRKMRENNISPRIFDSGLAVSLFRDNSTRTRFSFSSACNLLGLTVQDLDEGKSQIAHG
                     ETVRETANMISFMADVIGIRDDMYIGKGNTYMREVSKAVQTGYEEGVLEQRPTLVNLQ
                     CDIDHPTQCMADMLHLINYFGGVENLKGKKIAMTWAYSPSYGKPLSVPQGVIGLMTRM
                     GMDVTLAHPEGYELLSDVEDIARANAAKSGGKFSKTNSMKEAFANADVVYPKSWAPYA
                     AMEKRTNLYGEGKFDEIKALEKELLAQNAGHKDWECTEEMMKLTKNGKALYLHCLPAD
                     ITGVSCKEGEVAASVFDRYRTPLYKQAGYKPYIIAAMIFLSKVKNVPETFEMLMDSNY
                     PRFSGK"
     gene            complement(68684..70042)
                     /gene="hydA"
                     /locus_tag="PKI50_02150"
     CDS             complement(68684..70042)
                     /gene="hydA"
                     /locus_tag="PKI50_02150"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNT02551.1"
                     /translation="MDLIIKNGTIITASESYKADIAVKNGRIAMISGNIQAAGERVVD
                     AAGKLVLPGAIDAHVHLELPVGETVSADSYESGTRAAACGGTTMVFDFVTQEKGMGLV
                     ETADSRLGYVASRACVDYAFHIAVTDLTDKVLDEFKASVEFGVPSFKLYMVYKDMMVD
                     DGVIADALVRSKETGALVAVHAENPYIIDRRIKKLLSQGKTSAWYHYESRPEFVEADA
                     VRRMVYLARTFDAPLYIVHLASREGLEEVSGARDEGFEIYAETCPQYLNFNNEVYKRE
                     DGRNFVCSPSIKGEDSRRALWEGLRRGYISTVATDHCPFQSYEKDWGKDDFTKIPNGC
                     MGVENMYPYMLSEANKGNITFNKAVEVCSANAARIFGCAPQKGTLAVGSDADIVIYDP
                     EKKFIVTRENMHSDVDYTIWKDCEMSGYPVMTFSRGRLVYEEGSFLGEPGWGKFVKCF
                     RK"
     gene            complement(70093..71808)
                     /gene="ade"
                     /locus_tag="PKI50_02155"
     CDS             complement(70093..71808)
                     /gene="ade"
                     /locus_tag="PKI50_02155"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNT02552.1"
                     /translation="MSVEQKQKRIEIAAGREKADLAIKNASIVNVFTNEIISGDIAIH
                     DSLIVGVGDYSGREEIDAGGMYVCPGLIDCHVHIESSMITPAEFARVVLPHGTTTIIA
                     DPHEIANVCGVEGIHFMLNEARNVPMNIFYMMPSCVPATPFENNGAALTARVMEPFAG
                     SCHILGLGEVMNYPGVLAGDRDILNKIELYDNAVVDGHSSGLSEHELCAYKAVGVLTN
                     HECTNIDEVIAHLRLGMYVQIREGSAARNLEDIISGLIERDVPLNHCLFCTDDKHLED
                     ISREGHISCNVKKAISLGVDPIEAIKMATINPAKCYHLKRLGAIAPGYSADLLILSDL
                     NSFDIEKVLFRGSVVCEKGKKPDISRIIDDPNVLNTVRIPNLSPEHLKIKIKGDKANV
                     ITIIPNQLITKKERLAVPKDNGNNFIPDEVFSKIAVVERHKATGNIGLGIVKGFGIRN
                     GAIASTVAHDSHNLIIIGDNDADMLLAAKVLKEANGGYTVVSGGEVTGTLPLPIAGLM
                     SDRDSFFVENKLSGMIESAKSLKIPDGIDPFVTLSFLALPVIPELRVTDRGLFDVGSF
                     SFIDI"
     gene            complement(71805..72599)
                     /locus_tag="PKI50_02160"
     CDS             complement(71805..72599)
                     /locus_tag="PKI50_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HNT02553.1"
                     /translation="MEYAILEKLAAEVKANRKAALVTLVEEQGSSPGKKGFMMAVFAD
                     GSTLGTIGGGNLENKAREYALLCMSEGKNKLMEMELDDTGELHMQCGGKANIFIKVFK
                     AKDSLVIAGGGHIALELHKLGKLMGFHIVIMEDREEYGNNERFPGCEIHVGDIGECMS
                     KYPIDEDCYVVIVTRGHASDADALRAAIGKGAGYIGMIGSRQKTQFVFNRLLSEGCKK
                     EDLEAVYAPIGLGLGGDSHQEIALSIIAEIMLVKKGGSLKHMRDLR"
     gene            complement(72604..73407)
                     /gene="yqeB"
                     /locus_tag="PKI50_02165"
     CDS             complement(72604..73407)
                     /gene="yqeB"
                     /locus_tag="PKI50_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HNT02554.1"
                     /translation="MDDIIIVKGAGDIATGIGHRLHRCGFRLLLTEIEKPTVVRRFSS
                     FANAVLKGEAEVEGVRAVKAECIEDIYEIWNNSHIPVLCDSEMKILEQIKPLVIVDAV
                     LAKRNTGTYRGSAPVTIGVGPGFEAGADVDAVVETNRGHDLGKVIYKGTACADTGIPG
                     YIMGYSQERLLRAPVTGITRNTREIGDIVKQGDVVSFVDDEPVKSNIDGVIRGLIADN
                     TYVQKGLKIGDIDPRGIREYCFTISDKSRSVAGGVLEAILTLRKEKYGR"
     gene            complement(73400..73990)
                     /locus_tag="PKI50_02170"
     CDS             complement(73400..73990)
                     /locus_tag="PKI50_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT02555.1"
                     /translation="MISGIILASGLSRRMGTDKLLLPVGGIPVIERVIAAASKSELGE
                     IILVCGSDSVADIGKKYRAKIVENNAPETGQSHSVGLGVKNSCRSAEGFMFIAGDQPF
                     LDESIINKLIGKFLPGHCNAVVPLYNGIKGNPVIFASKLRNKLMSLSGDSGGRVLLEE
                     MKEGIITVNFADAKPGLDIDTWEEYEAITKLEGENG"
     gene            complement(73995..74729)
                     /gene="yqeC"
                     /locus_tag="PKI50_02175"
     CDS             complement(73995..74729)
                     /gene="yqeC"
                     /locus_tag="PKI50_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNT02556.1"
                     /translation="MESLYSQVNISIWKKEMICLVGAGGKTSAMFRLAKELSGTGKKV
                     LVTTTTAIYYPLENQNVQTVIADLLLPDLFKSIRSGITVYGRALSREGKLLGVDPEFL
                     DTLFLKDIFDYIIVEGDGSRGRPLKAPAEHEPVIPSLASRVLGLVGLDCVGKKVCPEN
                     VHRTEIFCNITGCREGDIIDADIIGRLIAHEEGLFKAVPSRAERYVVLNKADGEKEWS
                     AAGKIIQRLSDMGYEPDGIVVTKLGM"
     gene            complement(75006..76661)
                     /locus_tag="PKI50_02180"
     CDS             complement(75006..76661)
                     /locus_tag="PKI50_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNT02557.1"
                     /translation="MKGNILSNNGQLAADEVLQAAGGGIPAAYGAKEPFIADSRDTIF
                     AVLAFVLGFFFVRWVMFSWQGWGVAVFTFGYYIAVTMYFIKKGVTITRSGFFWLAVVV
                     LVGISYSLYSNNGLEPWRSLFLFCGAVYYVISASGRLILANTSNWLILDGINAMFVIP
                     FKNFGCQYKSLYSSIHIKRSFGKQFLSIALGLMLTLLIAGMVLPLLLRADSGGFSRIA
                     NGLIEYFRWMQNQFSETIFNIILSIPVAAYLFGLVAGCAHNRGCNTFKREGIQYGVQS
                     MRVLAAATVYTVLGLICCLYMVFIGSQLPYFFSAFAGQRPEGWQIYSEYARSGFFELC
                     QIAAINLTLLTLANLFCKKPQKNYILKTLNCLLSLLTLLLIAAAFSKMALYIGAYGLS
                     IRRLLPCLFMVFLAVVFVGVIVLQKRQFSIVRLSAFTGTLIMCALCLLNPDGFVASYN
                     ADRYLAGTLKSFDVQILYQSGPAGIDPALKVYSRTADEELKAELTEYIYHQQRESAKK
                     LGRTQDNLQSMLVRQKTAEFAELISGDSFKSYVPGTEQKNVLR"
     gene            complement(76685..76888)
                     /locus_tag="PKI50_02185"
     CDS             complement(76685..76888)
                     /locus_tag="PKI50_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT02558.1"
                     /translation="MPIIVNLDVVMAKRKMSSGELAEKIDINQANLSILKTGKAKAIR
                     FSTLEAICKALECQPGDILEFRE"
     gene            complement(76903..77340)
                     /locus_tag="PKI50_02190"
     CDS             complement(76903..77340)
                     /locus_tag="PKI50_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HNT02559.1"
                     /translation="MWSGRKSISLSKFCVLVFAVMLIGTVLSAPWLIRWFRPDLAEKK
                     ALFLSTIYSGSIPAAILLYCLYRLLHQIELEQVFITENVAYLRRISWCCFAGSVICLA
                     SIPYYFPWCFVAVAAAFMGLIVRVIKNVIAQAVELKNESELTI"
BASE COUNT        19632 a        19795 c        13966 g        23991 t
ORIGIN      
        1 agcgatttcg tgaaacagcc gtgtctcccg tgttacccgt tacctatggc gcattcttct
       61 tctattctgc aatgtctctg tgatactcct gcaaagattt aatcttcttt ccatgaaacc
      121 cattttttat atatgccgca attccctttg cgcttgccag agccgcggtc attgtggtta
      181 tatacggcac cttgcgcttt atagcaatct tgcggatata agaatcatcg aattcgctca
      241 gtctggtttg aggtgtattg attatcaggt ttatttcatc gttctccatg ttgtcagcta
      301 tgtttggccg gccttcgtat accttattga ccttttcaca gctgattcct ttttcttgaa
      361 ggaatttgca ggttccttcc gtagcaacga ttttaaagcc tatcctcgaa aattctctgg
      421 cagcctccag aacgtcaggc ttatctgcat catttacact aataagcact gttcctaaag
      481 aaggaagtaa ttcctgcgtc gcttcctgag ccttatagta tgccaattcg aatgaatccg
      541 agatgcctag tacttccccg gtagaacgca tttccgggcc caataccgga tcgacctcag
      601 gaaacatatt gaagggcaga actgcttcct taactccgta gtgaggtatt tttttgctgg
      661 caaggccgtc caaggagtat cttcctccca agcagtcagc catgattatt cttgtggcta
      721 tacgtgccat ttgtatattg cagactttgg aaacaagggg aacagttctt gaggcccttg
      781 gattggcttc caaaacataa accttgttat ccgctatggc atactgcatg ttcataaggc
      841 cgataacatt catttccttg gcaatccttc cggtatagtc aacaatagta cttatgtgct
      901 cttcagaaat acctacggga ggaataatac atgcggaatc tccggaatga acgccggcat
      961 actcaatatg ttccattact gtcggcacaa atgtatctgc tccgttggaa attgcatctg
     1021 cctccgtttc aatggcatgc ccgaggaatt tatctaccag aatcggcctt tcgggagtga
     1081 ctccaaccgc cgcagccata taggtgcata gcatttcctc gtcatgcact atttgcattc
     1141 cccgcccgcc caatacatat gacggacgaa ccattaaagg ataacctatt tggtttgcta
     1201 tggctatggc tccttcggca tttaccgcca tacccgactc cggcatggga atgtcaagct
     1261 tatccataat tctgcggaat ctgtccctgt cctcagccag gtctatggtt tcctgggagg
     1321 ttccaagaat ccttacaccg gccttttcca gatcccccgc aatattgaga ggagtctggc
     1381 ctccgaattg aactatcact cccagaggct tttccttctc ataaatgctt aatacatcct
     1441 ccactgtcag gggctcaaag taaagcttat cagatgtatc ataatcagtt gaaacagttt
     1501 cagggttgca gttgaccatt atcgtttcat agcctaaatc tctaagttca aaggctgcgt
     1561 gaacgcagca gtaatcaaac tcaattccct ggccaattct gttggggcct cctccaagaa
     1621 tcataacttt tggtttgtcc gttacggaag tcttatcgga tatgttatag gaagagaaat
     1681 aataggacgc attctcaaca ccgcttacag gcactgcctc ccaaccctct gtcaggccta
     1741 atccggttct tttccttcgg actaatgcct ctgacacatc aagaagctgg gccagatacc
     1801 ggtcggaaaa accatccttt ttggccttga taagcagttc atcctgcagt tcgcttcctt
     1861 tgcacttaag tatttcctct tcaagcagaa caagctcctt catctgctct atgaaatagg
     1921 gttttatatg cgtcagctta taaagctgtt caacatcagc acccttgcgc aatgcttcat
     1981 acataatgaa ctgccgctgg ctggtcggct cagtcagcat ggaaagaagt tcataaagag
     2041 tattattatt gaaattcccg gcaaagccca agccgtaacg attgatctcc agggaacgga
     2101 ttgccttctg gaaagcctcc ttatagcttt tcccgatgct cataacctcc ccaaccgcac
     2161 gcatttgggt tcccagttta tcttgggctc cggggaactt ttcaaaagcc cagcgggcaa
     2221 actttattac aacataatct cctgatgggg tatatttatc caggctcccg tccctccaat
     2281 aaggaatctc atcaagggta aggccggctg caagcatggc ggatacgtat gcaataggga
     2341 agcctgttgc ctttgaagcc agtgccgagg aacgcgaagt gcgggggttt atttctataa
     2401 ttacaaccct tccggttctt gggtcatgag cgaactggac attggttccg ccgattacct
     2461 caatagattc caccactgaa taagcgtact cttgcaagcg ctgctgaagc tcaggactga
     2521 ttgtgagcat cggagcggca cagaaggaat ctcccgtatg cactccgata gcatcaatat
     2581 tttcgataaa gcagactgta atcatctgat tcttgctgtc cctcactact tcaagctcca
     2641 gttcctccca gcccagaacc gattcttcaa ccagaacctg ccccaccatg ctggcagcaa
     2701 ttccgcggct ggcaatagtc ctcagttctt cagcattata taccaggccg ccgcctgtgc
     2761 cgcccattgt ataagcaggc ctgataacca caggataccc aagctcccct gcaattttct
     2821 cagcttcgga aacgctgtag gctgccttgc tgcatggcat atcaatccca agcctgttca
     2881 ttgtctcttt gaaagcaatg cggtcctctc cgcgttcaat ggcatttatt tgaacaccta
     2941 taacctttac gtagtattta tccaggatgc cgctcttata aagctccaga caaagattga
     3001 gagctgtctg gcctcccaga ttcggcagta ttgcatcagg cctttcttta tcaattattt
     3061 ccgtgaggct tttggtgtta aggggttcaa tgtaggtcga atctgcaatt cccgggtcgg
     3121 tcataatagt agcggggttg gaattgacca gtacgatttt atagcccaag tttctcaggg
     3181 ctttgcaggc ttgtgtcccg gaatagtcaa attcacaggc ttgcccgata atgataggac
     3241 cggatccgat aatcagaatt ttatttatgt cagttctttt cggcatataa atcctcctat
     3301 tactgtagtt aataagtcat ggatgtaaat aaggatagca aagagattca catatgaaca
     3361 ctccttgata tcccatgatc cggaaaaggt ctgcacatat atttcctcct ttctggcctc
     3421 gcaggaccaa tttaaagttt gaaattactg tttgaaaata taaacctata tgcatatata
     3481 taatataata tgtatattta tgcttgtcaa tatttttttg cctgtaagta aaaaaagaca
     3541 tggagacttt tcttatgccc ccacgccctt tgttactctg tccgttttat gtgtcctttg
     3601 aatatttgct tattaaaaat gttataatat agcccatgat gaaaagcaaa atgctgatta
     3661 aatattcaga actgaatgaa atgcccggaa atatcgaaat tatagatccg attacaaatc
     3721 caatcacggc ataatatgta aaaccgtatg cttttttgaa caaaaggctt atgaacttgg
     3781 caaatagaat tatgctgaat acaaatcctg tccctacagg tacaagaatt gacagattaa
     3841 tatttgcaag tccgtcaaga actatctgat atgctccaaa atacatcagc acaaaggaag
     3901 cactgattcc cggcactatc gtccctactc ctataattgc accgtataaa acaagctgaa
     3961 gcattcccgg tgctgcatca ggaataatgt cgattccggc attttccagc actactgtta
     4021 ttattgtgat tccaaatgca attatacaag ggataatgta tatgccttta aagcctttct
     4081 tgttggcctg cctgaaaact gtaggaagcg ttcccagcat aagcccgatg aaaaggcatt
     4141 tgacctgaac ctcatagtat tgaaaaaggt attgcattat tttgctgaaa ccgagtaccc
     4201 ccacaccgcc gccaagtgca atagggaaaa agaatactgc gtttttctta aaatccttga
     4261 agacatccgc aatggcatcg gttatcctct catataatcc aaatattacg gcaattgctc
     4321 ctccgcttac cccgggagca acgccgccta tccctatcag caatccctta aaaaagcccg
     4381 ataaaaaatt catttctcct cactcacctt tatgcttttg tttttaccat aggtatttcg
     4441 tataaaatta tttatttata cgattcgtaa agcacaaagt cttcaagggg cagtctgagc
     4501 tcttgatgcc tttccggaga tagtgctgca tctgcggaat aacctattgc aagaatattc
     4561 atcggttcaa ggttttcggg gatactaaac tctgctttga gtacctttgg attaaagtga
     4621 catatccata gagttccaag ctccagttgt gtagcttcaa gcatcatatg gtcagttaca
     4681 atagccgcat caatatcagc tacatctttt ccatcatagc tccttttcca tgaaatatta
     4741 tggtctgcgc acacaatgat agtcagtgga gctccatata tattagctga ttttctcagt
     4801 ttttctaggc tttcggcttc ttgaaccaca atcaatctgt ggggctgcct gttggatcct
     4861 gtaggcgcta ccctggctgc ttcaagtatc ttcaatagct tttcctcttc aaccttcctt
     4921 ggtaaatagt tcctcactga gtatctcatt ttagccaatt ccaaaaaatc catatcatac
     4981 accttccctt acaagcatct tttataatat tctacactaa ttcttcccaa agaaaaagca
     5041 ctgcattcaa aaagcggtgc tttttacaaa tctagactgc ttctgttttt cttatactcc
     5101 ctgctttatg cctgcgaagt tttacagaca catcatcaaa caatgtatag attaccggga
     5161 ttattacaag agtaagaaca gttgaagtcg taagtcctcc gataacaact gttgccatag
     5221 gtgcctgggc ttctgcccct tctcctatac ccagcgcaag ggggataagc cctaatattg
     5281 ttgttaaagt cgtcatcaat atcggtctca atctggtagg accggcttta agtattgcct
     5341 ccagcctatc catacccctg ccccttaatg tatttatata gtccacaagt actatagcat
     5401 tgtttacaac tatacctgca agcattatta ctccgatgaa agccggaacg cttaaagccc
     5461 tccccgttaa aacgagtccc aatacagacc ctgtaaaagc gagaggcaga gagaacatga
     5521 ttgtaaaggg atgcagcaag gattcaaatt gtgcggccat aaccatatat accagaacaa
     5581 ttgcgagcag caatgcaaga gtcaagcttg agaatgcatc tgcaatctgc tccgcctcac
     5641 cgccgtactt tattgaatac cccggtggaa gaagcatttc atccagcctt tcactaatat
     5701 ccttgttaac atctccggta ctccttccaa acacatctga tgaaacagtt acatatcttt
     5761 cctgaccctc ccgggttata gaaatcggtc ccttttccat tttaatatca gcaatctctg
     5821 taagaggtac ctcaactccc aagggtgata gtatctttgt atcgattagc tgcgaatact
     5881 tttcccccgt ttcttcggga aaacctactc ttacatctat ttcgtcccca tcaaccttgt
     5941 aggttgtcgc aacacttccc tcaacggctg ttcttattgc agccgacacc tgtgcagtac
     6001 ccaaaccata ataggatgcc ttatctctgt ttacatatat ttgagcttcc ggtcttccct
     6061 ctgaaatgct tgtatttgcc tgtcttacgc cctctacttt ttttattagc tcttcagcat
     6121 cagctgctat ctccgaaagc ttatccaaat ccggtccgca tattttgaca gaaaccgcag
     6181 tacctccgga tactcccata gccgatgtgc tgacttcatt gatctctata tcagctccgg
     6241 gaattagtct taccttattt ctcatctcct caactacctc tgcggtactt ctttttctct
     6301 cacttaaagg ctttaaggta atattcaaac ttgctgtatt tgatgcccct ccggataaat
     6361 ccattccgct tcctgaagaa ccaaccgttg taaatacctt ttgaatctcc ggaaggctgt
     6421 ttagcattcc ttcaacctgc tctgcgactt cattagtatt cttaagtaaa ctcccttgtg
     6481 gaagctcaat ccctaccgta aactgaccca tatccatatt ggggaagaac tcggtaccca
     6541 caaaaggaat cagcagcaaa cttcctgcaa atattgccaa cactgtaata actgtttttc
     6601 ttctatgtgc taatgcccag cttaatatgc ttctgtaaaa attatcaact gcacttagag
     6661 tatcttccca cttgtcaaac actttattta atctttcgct tcttgaagct tcgtgatatt
     6721 ttaccatctt caaaaatttg gagctaagca taggaataag tgttaaggct acaagcaaag
     6781 aagcaataag tgaaaaggtt actgccagtg ccatctgttt gaatatttct gctgctatac
     6841 ctccggtaaa tactataggc agaaatacaa ccactgttgt cagggttgat gccaatacag
     6901 cggaacctac ctcctgtgtc cccagcattg cggcctctac cctgctgtga ccttcattcc
     6961 tatacctgta tatattctcc aggacaacta tggcattgtc aaccagcatt ccaaccccca
     7021 gggcaagtcc tccaagggat acaagattta gtgttatacc ggaaaaatat atcattacaa
     7081 aggttgctat tatggaaatc ggtatggacg tcccgattat catagttgtt cttatattct
     7141 tcaagaacac aaaaagtata ataatagcca gtatagctcc tacgacagca ttttttgcaa
     7201 cattcgctat tgactgctcc acatattctc cctgatcaaa aaccatttca acctttacat
     7261 ccggatattc cttcaatata ttatttattt ctttctttat aagattaact acttttaccg
     7321 tattggaatc tgtctgcttt tgaaccgtaa gtcctatgct gctctcacca ttcattctgt
     7381 tatacgtatc aacactttta tatccttctt ttacttcagc catatcccgc agatatacaa
     7441 ctgctcctgt gggaagcgta atggggatat tcctgatttg gtcaagactt tgaaattctc
     7501 ctgtgcttct gactaaaaga ctcttatctc catattccac tgtgccccca ggctggttaa
     7561 gattctctga tctgagagct ccgctaatct ggctgaagga caatccatat cctgataatt
     7621 tttgagaatc caaatttact ttaatctctt ttgtcgtacc gccggtaact gcaacagatg
     7681 caactccatc aatgctttcg atttttgctt ttatcttgtc ctcaacgaac cttgtcagct
     7741 ctgcttcatt ctcgcccccg gagactccaa gtacagctgc gggaatcatg ccgggatcca
     7801 tctttataat cataggggct tctactccat ctggcagcat tccctttacc atatcaatct
     7861 tttctctcat ctgcaatgtt gcgaagttca tatcggttcc cgagttgaat tctgcaataa
     7921 caatagattg cccttccgag gattgtgatt gtattgtttt tatattattt accgtagcaa
     7981 ctgcactttc tatattcctt gttataatat tctcaatttc ctgaggtccg actcctgaat
     8041 agcttgttaa aactatagat ataggaaaat ccatttccgg cattaaatct ataggaagcc
     8101 tggaaaagga taccccccca gaaccaaaac catcagcaca cacattaaaa ttgctacagg
     8161 tcttcttatt gacacttcat gtattttcat ccaaacacca tccctaattt tcggtaacta
     8221 taaccttgga gccatcaata atcagttcct ggccctttac gattacagca tcattttccg
     8281 caagtccttt tactatcaca ataagctcat catcaaacag acctgttgaa acttcccttt
     8341 tttctgcgat atcgccatta agaacatata caaatctctt atcgccctct tcaattacgg
     8401 aggaaatcgg aaccgctata acatcagaca ctttatccaa tactgttttt acttctgcaa
     8461 acatacctcc cttaagagtt ccggagctat tatctatctt aattcttaca ggatagcttt
     8521 gagttcttgt atctattgtc ggagatatat taattatttc tcctgtaaat ttactatctc
     8581 ctactgactt gattgttacc tctgccttat ccccaacctg tattttattt atcatacctt
     8641 cagttgcatt tatatcaatg agtactgagg ataaatctat tacactcata gcaataccgg
     8701 tgctgcctgc aagctcacca atttcaataa ctcttgcagc aattactccg tttataggtg
     8761 atttgattac cgtattgtcc aattgggatt tagcaagctc aagagctgct gctgcctgac
     8821 ttacctgtgc tttggccgat gcaatgtttt caggatttgt ttttgttgtc gtaatctcat
     8881 agtttgattt tgcagaatta tactgctgtt ccataagctt aaactgtgac tccgactgtt
     8941 ccagtgttgc cttagatacg gcaccggatt catataactg ttttgttctc tcaaaagcta
     9001 gctttgcatc attatagctt atttctgccg aagtcagtgc cgctttaagc tgcagctcct
     9061 gcagctctgc tcctcctcct gttactttag ccaatcctga atttgccatg tcaagccctg
     9121 cctgagcttg cttcagctgc gaccttatat cattatcctc caaagtaaac aagacatctc
     9181 cggctttaac actttgccca atgtcaaagt atatattatt taccttcccc ggtaatttgg
     9241 gaataatgtt tatttcctga atcggcttaa gttttccgtt caaattgaca gttgcactta
     9301 cacttgtttt agctgccgct tcaacgctta ctgcaacgac tgtatcagca tttatatttg
     9361 cagagctgcc agctttgttt ttcacatatg taaaaattag cgaaccaagt aaagtaacga
     9421 ctactatacc tattatcagt tttgtatatt tccttttcac tatttaaacc cccatactaa
     9481 atctattaat tttcactact ataactgtct ataatatttt gagtaatttt ttcaattgtg
     9541 gcaaaaaaat tatttatttc ttcagtatca atatttgaaa aaattttctc ggacagtcta
     9601 ttcttgttag cctcaatttt ttccagaacc ttttctccct ccggcgtaag ctccatataa
     9661 acaacccttc tgtcctcaac gcttctaagt cttgtgacca atcccgcagt gcacagctta
     9721 tcaacaagac ttgttacagc acttaatgta atttccagat gatttgctat gtccgttaca
     9781 gtacattccc ccttgcgctg tatatatgcc aaaagataga catgagactt gcttaatgat
     9841 ggttcaatat ccttaagtat cacagccatc agctttttat gaatatccat ggctatcttc
     9901 atcatcctgt cacatttatc ttttatatcc aaattaataa acctccttaa ttattaagcc
     9961 acttaactat aaaacaattt tattataatg cagtagaata ccaattgcaa catactgctg
    10021 taaaaatttt tgagtgaatt ctattaatgt taccgctcct aaggttattc gatttagttc
    10081 tcaatagcat ggatttatta tgctataata ataatattat tcaataatga cattaccggg
    10141 aggtacaaca tgctgtacat aagaaatgaa aacacaaatc ccttttttaa tctggcaatg
    10201 gaagaatatc tgtttaacct gaatgataat aatgattatg tacttttatg gcagaacgag
    10261 ccgacaatag tagtggggaa atatcagaat accgcagaag agattaacag tgaatttgta
    10321 aaagaaaaag gtattcatgt agtaaggaga ataaccggag gaggtgcggt ttaccatgac
    10381 ctgggtaacc ttaattttac ctttataaac aaaggaacgg gtaaaaagga gtttgacttc
    10441 aggaaattta ccatgcccat tgttaaagcc ctggaacgtt ttggtgtaaa ggctgagctt
    10501 tcaggccgaa atgatattac catagatcaa aaaaagtttt ccggaaatgc acaatatgta
    10561 aaacagggaa aggttctgca ccacggaacc ttacttttca attccaaaat ggaggaactg
    10621 ataaaggcat tgaaggtaac cgaggataag ttccaatcca agggaataaa gtccgtaagg
    10681 tcaagagtca caaatattgc ggattatctg tccgagaata ttacagtttc tgagtttaaa
    10741 gagctgcttt taaaatatat gtttgatgag gacacggaat taattgaagg acagcttaaa
    10801 gatgcagaca tgaatgaaat aaacttgttg atgaaaagca aatatatgaa ctgggattgg
    10861 aattacggag cttcacctga gtttaatgtc aaacaggcaa aaaggtttga aggcggcaaa
    10921 gttgaagtat tgataaacat aaaaaacggt gttatccaaa gcataaagtt ttatggagat
    10981 ttcttcggta gtgaaagccc tgaagaaatt gaggctttgc taaggggaaa acgatacaat
    11041 gaagatgaaa taaggggtgc tatcgccccc ttgaatatta atgagtattt caaaggcatc
    11101 agccctgaag aattattaag ttgcataata taagaagaat acgacataat gagtttgtga
    11161 agtactccga ctgtagggga agtatgttct cctcaccttc caaagccagc aaaatcactg
    11221 gcgggggtcc catctctgcg ggtccgtctg gttattttgc ctggaggtat tcccgttccg
    11281 aggtcaggtg gaaggtttcc tcgaagatat cttccccacg tctgtatttg catttccttt
    11341 atatacaagg cagtgctctg taagatgcat actaaggagc actggtcttg tatagcagcg
    11401 agcacttaga agcctttggg ttacagaatc cagtgaagat gcgcagaaaa attcgtaagg
    11461 tttacgtgat gtaaacctag cacataccaa caggaagttg ggtttatgtg cgttagggtt
    11521 tttcaagcat caagctctga ttctgtccaa agcttatatc gtgcgagtcg atatacaagc
    11581 ctattgctct gggagtatga cagcaaactt aatactatgt tgcattattc ttctgatgtg
    11641 caatagtcct tactgcagtg atatataaat atcaaatatt tttgccgctt ctgcccgggt
    11701 cagcactctg tccggcctga aggttccgtc tgaatagccc tgcatcagct ttttcttaat
    11761 aagctgatca atatagactg cagcccattg aggaatttta tccttatcgc tgaattcagc
    11821 ttttatccct ttatcagctg cagaaagctt taatgctctg gataccattg cggcagcctc
    11881 ggctcttgtt attgggtcct gaggcttcat atatacttta tcccctatcc cttttccatt
    11941 tatccatcca ttgctgctgc aatacttcaa aaatggccgg gtccatggct gaatatccga
    12001 atcatctgca aagccggata aatccgtatt tacaccagtt tgcggactac cttcacaaag
    12061 agcaagcatc tttgcaaatt ccgctctggt aacggttctt tccggttcaa accggtaatc
    12121 tcccgaaggc agcttgatcc cgtttactat tcccctgtca ataagcctgt atacggcatc
    12181 cctggcccaa tgcccttcca tatctgtcat tacagccatt ccgctgtttt tcataactgc
    12241 atacttgctg aagtgaggaa ttattacggt aattgttccc tttccctcgt caacggttcc
    12301 cccaactaag tcccagtcac cagttttttc gttgtacaca tatattccga tttttttaat
    12361 atctttgact ttatttctat caaagataat cgtaagttca atcggagaat taaactggta
    12421 accctcaggt atcctgatat ccattatgct gcttatgaaa tccttattac cgctttgctg
    12481 cctgtattgc aataaatcct tgatttcata tatctctaag tgcccattgg caaatatctc
    12541 attgccggtg aatcttagct gaattagccc ggggacggtt atttcatcat tcgcattgaa
    12601 atccagagat ttccttagca ggcttcccgc attaatgccg tttgtatccg tcgccttggg
    12661 gtaatcttcg cggctatcct catcatcacc ccgatttgac gcatcagaca ccacattgat
    12721 aatcagcgca tcttcatagc tcatatcccc ggacagcgag tattggcctt ttattatagc
    12781 ttcacccggc tttaccgcag tcaacaaacc ttccggagta atagttgcac ttcctccggt
    12841 atctaaaagc tgccagtcgc ttttttcagc ctccagcaat acttggctgc tattatttgt
    12901 gataccgtaa agcttcagct gtatactttc tcctaccctc agctgctcta tctgaggtat
    12961 tatctgtacc cttactacat ctccaagatc tgaattaaat acttcggttg aatattcagt
    13021 tctgttgcag gccaaatcca cagccgcaat ggttattgtt tccctctgtg tatctgaaag
    13081 tataaagtcc tcgttaaagc ttccgtcttc tttcacagtc atttctgttc catttacata
    13141 aagcctgctt cccggtgttg tctttcccga taccaatact tttcctgatt ttatattgtc
    13201 actgctgttt ggactttgca ccatcaagtc cggtgctttg ttgtcatagt agaattcgta
    13261 agacttcaag gttttgtcac catttgcctt tatagcctct atctctatca gattgcttcc
    13321 gtcattaagc tccagttcct gtatgatctc cttgcctgtg aaatcagcca catcatctcc
    13381 gttaacatag aataccacat ctgcatttat atctgactga tagctgcaaa taaccgcatc
    13441 gtaattggta tatcttatat caatatcgtt ttcgtccttt tccacattta tgtttccgtg
    13501 ctcggaagag aagcttgcag taaactcagg aggctccggt accggaagat taaccgaccc
    13561 tgtatatgca ggcctgctta tatgctctat ctccatatcc ttatccggat cttccggatc
    13621 cgaaactgtc gatttattta ccgcatagat tgcaaacctg tatgatctgc ccggtatcca
    13681 gccatattcc aactcttcgg gacttgtctc agtctcatca tcctctgact ttttgggaat
    13741 tatctgtgtc ccgccaaaaa tagtacttgt ttcggttcct tccacgctta ccatagcttt
    13801 tacagtatta tcaatcttat tgctttcatt cacaggataa atccgatatt catccgcacc
    13861 atctgagaaa ttccattctg cttccatcat gccgttgcct gcagacttca ccgtaaagcc
    13921 cgttggagta tccggcgcct cggcatcaac tatctcaaag gccgctgtat tcatttcgtc
    13981 aaatgaaaat gtatcctcag catcgatacc ggttcttctt acctctcccc ttaccttata
    14041 cattcccgtt gtcgcacttg cgggtatatc acattcgaat gtccccaatc ctccgggcag
    14101 ttcctttgcc agctcaatca tgcctgatgt gccgtcctct tttacaagat acaatgatac
    14161 cgtactgtca tcggttacgt taagctccca ggtcgtcctt attttacccg cactctgctc
    14221 tgcagaaatt gattcaaaaa cagcgggggc cttgacttta catggtgtca cagcaagggc
    14281 ccgatctgat tcgattaacc attcccctgt aatgggagaa ggaatggtta caaataaata
    14341 tttttcttct tctcccgaag ccgatttatc tgcgggaata atctgattcc tgtaatttac
    14401 accatcggcc aaggcatatg ttgttccgtc aggcttagta accttaatat ccggcgtttc
    14461 ggaagaagta tatttcatct ctattattgc tgactcaaag ctatcgtcaa ttgttacatc
    14521 ataattatat tcaaaggtgc ccgccagtat tttacccata ctgccggcat agcaaacatt
    14581 atgggaggga taggacggct caacttcctt caatctttca atattcgaac cgtatgtgac
    14641 cgttacatcc ctgccccggt tatccttcgt tctgactgta taaatccctt tatcgtcagc
    14701 atctgaatta gatcctacat caaaatcaac atcatggtct ttccatgtat atgttatccc
    14761 gactccgact cccatgaaac tgatgctgcc tttaattttt tctgtggaca attcagctcc
    14821 gaagcctgca atctcttctc ctcctatgaa ccacacatag tcgggtacct gaacctttgc
    14881 gtttattgag cctttaattt catggctgct tattccaaaa tatgcatctg caataagaat
    14941 gtcaagaaca ttcaaatctg cctcaatata acctccgctg aaatcaaagt ggccgataat
    15001 atctcccaca tcaatgaaat aaatatatgc atctccctcc aggtcaatac cgctgctgct
    15061 aagtattgcc tcagtatcca gcagtcctat caaagtgtat ccctttattc tggcgtgata
    15121 agtaaacagg tctacaatat tcgctcgaac tgctatactg gagcctgtag gcagaatacc
    15181 gtacttcagt gaatcaatca tggacttaag gccatatata ccgccgccta ttctttcaaa
    15241 acctagtccg gtacttccga ttggcttcgg tatttttcca taattattgc tgaactcaaa
    15301 atagtcggga tacagattct tgtcaaaggc catttttgca tttatatcca aaagcctgag
    15361 ctttatgttt tctgcctttg tctcgaagcc ataggctcct tttattgtgt ttataaaaaa
    15421 tgtaatctgg gtcatagatt ttgttgcata attctgtgac tggaagcctc ctaacttcca
    15481 tcgggggaaa ggtatttcaa tttcagcatc aatatcgatt tttttattgg tcaggctcaa
    15541 atcctttaag tccgatttca cagatcctgt cccaaggcct gtcagaaagc tgaaatactt
    15601 gttctcaagc tgcagcaatc cctctatcct gacttctccg ttattttgga tttttatctc
    15661 ctttacaatt atgggaaagt atcctacttt tattgtagtg atatcgttga attcgccctc
    15721 ctgatccctg attctggcag tcgccgaatc aataagaaaa taccccttct tcaaagtgac
    15781 ttccatgctg cttcccataa gacaagactg catgtatagc tctccgtttc cctttactgc
    15841 atagccctgc ccgctcttgt gaacagacag cacattatct ccatagccct tataataaat
    15901 attgttatta attgctatgg tatcaccgta tatctcataa ttatccgcgg aaatatcttt
    15961 tatctttcct ctgataatca gctttgaatt gcttttttcc gccggggtga gctgggattc
    16021 attttcatac agttttacac tttgtactcc cccattatcc tcctttattg ccataatccc
    16081 gtagtttctt gacttatact ttatatcaga ggtcaggaat agttcctggg gcaaggtata
    16141 atccagaata tcctccctat ggttaatgga tatcctgtac gttccgacag caaaaccttt
    16201 aggaactttt agacgtatct gattatcatt tattatggta atatcctccg gggaaaaata
    16261 atgaactatg ctcccctttt tgtccataaa gcccatttca tatccacttt tgtccttaag
    16321 cttgtcaaaa ccatggccta tcagctgaat ggcattattt tcatcttcat agtacagatt
    16381 ccgcggaact gcatcggtat acccgatttc aaagttattg ttctcaaatc ccggtatgat
    16441 aatgaacttc ttgattgttt taagttcctg gctcatacct gtgattcgta ccatgtattg
    16501 aagcaccctg agcttttggc tgtttagggc tcttaccttc cacttaactg tttctttccc
    16561 gctaatattt attttataga aatatttact gctttcttct ccttcctcta tctccagccc
    16621 ctcaggcaaa atcagctctg cggttatacc cgccagctgg gttgagtttg gaaggctgtt
    16681 gtccagctcc attattattt caaaggggtt gcccttgtat ttatctcctt cgatagtaag
    16741 cttggaaggg accagcacat tcatattgaa ggtgcttccg tcactgtttc cgatgttccc
    16801 caagccgtaa tatgtctcca cttgcattgt ctctcccggc tcaaggctct ttggttccca
    16861 ataaagggcc actgccgaat ctgcactctt ataatcattt aatatagaac tgatattcct
    16921 gtccctattg attgaataat cccatttagt cgaggacaat gaactccaat gtgcaatgat
    16981 cattttgtcc ggttcggtat tcccccaacc cttaagaaat ccataggaaa caatctttgg
    17041 attttctgca gaatcaacag tcctccaata taccggaata tcttctccga taacttctgt
    17101 ctcgaaggaa atgtcggact tcccatccag ggaaaccttt gatccgtcat tatccccaag
    17161 catggtatcc atcaggattc ttgttcctat taacttgctg cttgtccctt tgttggtaat
    17221 aatatagctt atcttaacat tgcccacatc actgctggtc acattatcag tcatttccag
    17281 cttttgggtt atttctattc cctccggctt ccaggttgag gtgttgacca ttccttctgt
    17341 ctttggagta tctgtaaagc ctccatccat agttaacccg ctgtatgaat tgccgtatat
    17401 ataatccttc ccgtctatcc tgaatgtagt aaaggtggat tcaggaattc ccttcaggta
    17461 gagcagggat ttgttgtcgt cctcaggccg attaggagac cctgcaaccg tctttattgc
    17521 atatctgccg tcatctccat tgactaacag ctcaatttta tcgttcttaa tagagtatat
    17581 tttcggcgtt gcggcattga cggcttccag tgcaattccg ttaaaaatac tcaaggtcca
    17641 aattaccaac attaatgcgg attttaatct ccttctcatg tccccacccc cttactcatt
    17701 gatatacact tgaatacttt ttgttctccg ctccctgtcc tgtataaaga aggtatacca
    17761 tctgccggtc tccagccaga cattggtgcc ttccgcaaga atattcccgt ttcccttaaa
    17821 ccaggctatt gtccttctgc cttctcccca tttaatcaca aagtcccctt catttccaag
    17881 ggtatttgct ttaatgttct ttgcatctaa aagcatcatt ggtccctctg ccaccttgcc
    17941 gttcagcagg agcaccagtt caccggattc taatactcgt acagtcctga tttcctccgt
    18001 actattgccc actgcatcag aaaccttgta ttttaataaa taggttcctg cctctgcggt
    18061 atttacgctt ccttctacag ttaccttgcc ggttatatct ccgtcaatat tgtcggtagc
    18121 tttgtacccc gcttccgcat aaggctcatc aagcattatg cattcgtaca ggtttccctt
    18181 caacgctatt ctcggccttt cttcgtctat attccatacc tccgccttaa ggcttactat
    18241 attgcctgcc tcatcagtaa tcctgaaagt gaaatatccg tttgtataga aggttcttgc
    18301 aggtattcct ccgttattta taatagcagc atcggaatca gcctttagtg tcacaacaac
    18361 cggtctgttg ctcatttctg tatggctgta ttctaatgac cctttgggag gaagcttgtc
    18421 tatccagttt accgatgcag tctttgaact tctgtttccc gcttcgtctt caaattcaaa
    18481 ggtaaactct ccgttttcgg taaacatgta ttctatcttg ccgttattgt tcaatacctt
    18541 tccgggttcc tccaaattta atacagccct gacatcctgg tttgtgatgc tgccaggagt
    18601 atattctata tttcctacag gcgctatcct gtcaatatag tctacaataa attctttggt
    18661 tgtctcattg cctgcagcat cagagacctt tatccttatg gttttattct cagtgcatat
    18721 ataagtcagc tttccttcag aagcggtcaa attctccgct tccatattat gatccacgta
    18781 tacagttcct ttgtcatcac tgtagcttac tgtaagtgtc acatcacttt gggtaggata
    18841 cacgatgtcc gcaagaatct gaagctttgg tgctgactta tcaattttac ttaccgcagc
    18901 ttttgcaaaa cctctgttac ccgtgacgtc ttcatagaca aatgtaaact ccccgttctg
    18961 ctgaaagaca tagctgtcgc tgccgccgtt atttgcaatt gtataggatc ctcctgcggc
    19021 ttccggaatc agttttgcag tcacactttt gtttatcggt tctaaattag gatcctcaac
    19081 cttatttccg tcaatatagt attctattgc agacggtgcg gcagttcctg caaaaccggt
    19141 tacctgtgca gttgtttcgc ccttattccc tgccgcatct gaatattcaa atgcgaaggc
    19201 accgttttcc acgaactcga aggtatccga gccattatta ttcaatattg ttacttcctc
    19261 tgtttcctcc ggaaaggata tctttgccga aataatgttt ttatcggtat ctctaatgta
    19321 ctcaatttca cctgacggtg ccgtcttatc aatatttgaa actgtaactg ttttataacc
    19381 tgtatttccc gcttcgtctt caaattcaaa ggtaaactct ccgttttccg tgaactccag
    19441 tgtgttggag ccgttattgt tcctgattac aactgttttc ccgcgatcgt cgtacgttaa
    19501 taatgctgcc gtaacattct gattggtaag ctctgtctca cttaatatca cttctgcctc
    19561 cggggctgtt ctgtcaatgt tatacacact gcctgcaaca gtccttatat acccttcatc
    19621 ttcgccggac aacactctta tagacacagg ggactctccg ttttcagtga actcatgggt
    19681 atatccgtac acttcttccg caacagtcgg acttgtgata attgccttac ctgagaaagc
    19741 tttaaagcca gctcttatgt tttcattggt cagccttcct gcatcactgt atataatatc
    19801 agcatatttt tccgcttggg taatggtatc tattacggca gttgccgttc cggtatttcc
    19861 tgccttatct tcgaactcaa aggtaaagct gccattttct tcaaaaacat gtctgaaact
    19921 ttcgttgtta tttgtcacct ttaccggctc attaacggac agatagacgg taaccggtgc
    19981 agtatccgaa ggagtatata taatacttcc caccggcgga gtattatcta tgttttttac
    20041 ctctgctttt atgctgcctt cattgcctaa attgtccctg aactcaaatg taaactctcc
    20101 gttctcgtta aaggtatgag tatccccacc ctcgctaagt atgacacaac cgttttcatc
    20161 ctccatttga agctgtgcgg taattgactt attggtccat tgatctattg aagcgataat
    20221 tgacggttcc tcagtccata catatttaac ggctgctttt gggggatcct tgtcaatgtt
    20281 gcttacttcc gcctttaccc gtgtcttgtt tcccgcttcg tcggagatta tgaattcaaa
    20341 ctcaccgttg caattgaaaa cgtattcagc agattgattg ttattaagta taatcaccgg
    20401 attgtcatct tctgttgtaa gtgcagcctt tacatctccc tttgttgctg cagtcacatc
    20461 ataggttaca ctccctatgg gcgctgtcct gtccagtgtt atcaaatccg aataaatatc
    20521 cgaaatattg cccacctcat ccatgaacct gacaaataca ctctgctcgg cctcatctgt
    20581 attagtaagc atccaattaa aataagccgt gccgttctca aaaataatat ccttccaggc
    20641 ttcatcatca atagaagctt cggacagaag agaaccgtca tcagccagta cggttttgaa
    20701 gaccttgttt cccgcttctg caagctcatc tacagtaagc ttcaaagaca cttcaaggct
    20761 gtttgtaaaa tccttctcaa agctgacgct tccggcaggc tttgtcgcat ctatatcaaa
    20821 gacttccggg caatggacaa tctgcatatt tcccaaagca tcctgaagca ttacggataa
    20881 ataccatttg ccctctgtaa tggaatcttc cggatatgcc acatctccga aatgtatcac
    20941 ctctccggaa tctgatatag tgtcaatatc tatccatgtt tccggttcgc cccccggagt
    21001 atctgatatt ccaaatccaa acaatgagat atcgctgtac tcatccttta gtcccgaata
    21061 gctgaacata acagtattta acttcttgga agcatcgcag acaatcgtca cttccggagg
    21121 actgttgtca aaacagaact ctacagggtt gccgtctata gacaaaattt cttccaaaac
    21181 attttcggca taatctttta cctttatttt aagataatat ataccgttga ggttttcgtt
    21241 accatatact tttattccgg gaaactgatc attttcagtc tcaaagtctg cggatataac
    21301 ttctctgctg tctgcatcta tccaatttac agatacttcc tcatttaaca aatttgctcc
    21361 tgatatattg tcaattacat gcactttaac actatggtcc ctcttataat tctccggttc
    21421 aaaaggctcc ccatcagccg atcccatcag tataagctcc gatggttcaa aatcatacca
    21481 tattgtattc ccatactcgg tataattgta aaattcatca taatacctgg taaccagctt
    21541 gtaataccct gatagttcaa caccatcaag tactcccatt atatttgcag tccgttcttc
    21601 tcctgtgcct tccagatcaa ggaccttcca catgccgctg tctttaatgc cttccgatat
    21661 atcaggttca gcaatgggtt caaagtccaa tatcccatct tccatggttg tcagcaaagc
    21721 atataagaac ttgttctgca aagctctgtc cttcacctga taatgtgctg atgcaggttt
    21781 cccgtcaata tctttaaact cactataggt aacgcggggg ccttcacaat cgaaataaaa
    21841 gccctttctt ataatcactt cttcatctgt tccgttcact gccagtatat tcaaataata
    21901 tataccttct aatagagtat cttcccttac tgtttcaatt gtgtacttac catcctctcc
    21961 gggcaatacg acttttttga agctgctgct gtcgatgttg tatgcttccc tggtccatat
    22021 gtatttaacc tcatccacac cgctttcaat atcttcagca ctgaaggaaa ctgtacggct
    22081 tgtagtataa agagtgtctt cggatgtact gatatccact tctctcccat cctcatgaat
    22141 actcttatcc catgttatta ccggaggggt actgtccttc tgattgacct ttccgtaaaa
    22201 gaatctgtta ccgtaagggc tccttgcttc atcatttact tccgcataca gccggaattc
    22261 ctgataagga ggtattttgc ttccgttggg tatggctaaa gttacagtat aagttgtttt
    22321 ggttccatca ctaactctat gagcagtagc atcattcaga actatgcttt tttcctcttc
    22381 tcttactgca aagcctgttg aattcatata ttgccaggag taagtcatag taaggggaga
    22441 ctgttctctc cccatttctt cttcctctat aactatttcc accgacgatc ttcccggggc
    22501 gtattcctca tagctgtccc acgatgtgac tataacagga gaagcattat caaattttat
    22561 actgtaatgc tcaatatttg gcttcattat gtcagagggg ccctgatttc ctcccagatc
    22621 ctgcacagca taatcaaagg ggcctgctgc tccgattgac tcaaggctgt ccataaactt
    22681 gattttgtta tcttctccgc tgatttgaca tttatgaccg agaggtatgc tgtattgatg
    22741 tgaggttgaa tgtgctgtgt aatgccagga tgtactgtct atttctgcgt ataccctatt
    22801 tccatattga tttaaaacat tagtattcag agtaacctcg ggatagttta ctctgacagg
    22861 ctcattaaat cttatattcc catatatggt gtccgcagga ttcacccagt tgtccagata
    22921 ttctccggaa actgtatcct ttttcaatgg caccaaatta ccttgccagt ctcttatctc
    22981 tgttaccgtt gcttcggtca tgacaggggg attttcatct acaagccaca ataatatatt
    23041 atccagatat acatccagat cactgccgct ctccctaaat gccgttatga cagcctctac
    23101 ctttacagta tccgggggaa gagcttttgt cggcatgtta agccgtctcc aatacttttc
    23161 tccgacataa ctgcttgaat tgaatattgt agaccatttt ccgtccgctt tgatgctgta
    23221 tatgcttact acaaacctgt cctcatcgtc actatcccat ccgaaatagt ccaataaaaa
    23281 gttgacctgc agctgattca ggctgcacaa ggttctttga tccgcattca aggtaaccgt
    23341 tttggaaata actgcatcac tcatttccct tgttccatca cctgcatgct tgaaataatt
    23401 gaaggagccg ggattaccgc ttgaagggcc gttcttcaaa gaagtggcag aagccagctt
    23461 atttatcaca tgctcccacg agcccggtga gcttccatca tcatttatca gatatatatc
    23521 ccatcccccg taaacattta tccctgataa tgcccagcag gttgtcagca ttaatgtaag
    23581 gaacagaaac agctttatct ttatgttttt caacccaatc cctcctaatt gttagaggtg
    23641 cctttttatc tattatttaa ttatccaaaa attctgtata attaattata ttgtatatcc
    23701 tgtacagaaa cactgcagct tctgccctgg tagtacctgc cccaacattg agctttccgc
    23761 tgctgccttg taccagccct tccataacca ggcctgcaat gctttcaacc gcataacctg
    23821 ctacttccct tgcatcagca aacttctcca ggtcttttga tgttcccgta ctgcttatct
    23881 tcttcagatt ccttaaagct ctttctgtca gcacaagcat atcctgcctc gagataatgt
    23941 cctcaggctt gaagctgttg ttccctgtcc catttgttat tccaagcttc ttagctattg
    24001 ctatctcagc ataatagacg ttatcctttc ctacatcctt gaaattgctt tctgcctttg
    24061 ccgttacccc caaggtcctc accagagcac ctatgtattc tgcccttgta attcctgctg
    24121 ccggttcaaa gctcttttct ctcctgcctg tcattatgcc ctttgctgcc agtatttcga
    24181 tctgtttatt cgcccaatca taactataaa gatcattaaa agtctttatt acatatacta
    24241 ctgcgtatct gctgaaatgg gcagtagtaa aggtaacctt tcctgtcgca ggattatacc
    24301 tgccgcttgg tacagacaca gcatttcctg ctccgtcaat ataccacact gttatatttt
    24361 caggattctt cagttcttct gctgttggca catagggtat cgataccgtt acaggcgcat
    24421 caggattgct ccaggctatg gtccttccgt ccagcttcag ctcaagctca ataaccggtc
    24481 tgctgcctat ctgtgcctgc aattcggcag tcagcccgga ggtatctgcc tttccgataa
    24541 ccagttgaat tgtttgtgca gtacccagca tttctgccgt cagcatgttg gacggaatta
    24601 tcaacgttgc cggcccgttc tttacttcct tcatgcttcc tgaagctcca atcaaatcgg
    24661 ttacaggtat atcaattttc cacgcagctg ttggcgccgg tgtcctggat cttacattgt
    24721 ctgaaggagc ctttaatgtt tttgccgaaa cactgttata acatattttg ttgcctgcct
    24781 gatccctgac tataatgttc aagtaatagg tggtttcgtc attaaggcct gcaacagtat
    24841 agccggtact tcctgttata ttttcattga ccgcaacagt accgtttgtc tctatacttt
    24901 caactgaatc cagattattg gtataggact tatatataat atattcaagg ttggcagcat
    24961 cggtaaagtt atcggctgcc tcatcccacg tcagtcttat gcttctgtat gtaatatccg
    25021 ataaagtgat atctctgctt gctgcgacag gctcggtagt atctacaaat atatcctcgg
    25081 gaaccgatac attctcagcg ctatctataa gatatatctg ataatatccc tcagtaagtc
    25141 ctgtgctttc aatactgaca gagcttgcag cctctagttt ttcaatatat acagcttcaa
    25201 gctgggcttt gcttgtataa gccgttgacc tctttgctgc caggtagaca gacccgtata
    25261 tcccgtaatc agaacaggca acagttacat tacctccggc tttgattacc ataggagtaa
    25321 cctgtgaaat ttccggcttt gtatcgtcca gagtcatttc aacactgatg ccgctgcttg
    25381 ttgtaaaaag cccgctcccc tctaccgctg aatatccggc cttgttcatg atatatgaat
    25441 atgaggctcc tgacctatcc ggtacaaata tccaatataa tccctcttcg tctgtcaagt
    25501 actcgccatc aaggtcttct gctccttcaa gcctaatatt tacgcctaca gcaggtgaag
    25561 tatcgaacag aagataggac agattattgc ctgcgccgtc ccttaatgta cccatatcca
    25621 gctcttgaaa tccgcctcca ttgcctatag gctttgccgg atactcattc caaccgggtt
    25681 ctacatctat tgcaattaca gtcggtgtat tcagtatagt tacatcagct cccgttccat
    25741 tgcttcctcc gccgattccc gctgcattaa aacctccggt tgcctttacc gttcccccgc
    25801 ttatggtaag agtacctccg tccctgtttg aacctcctcc aatgcctgct ccgttgtttc
    25861 caccgtatgc ttcaacagta cccccattta tgacgacggt tcctccctct ccgaactcac
    25921 cgcctcctat gcctgcagct gtacttcctc ctcgtgctat tacagtaccg ccgtttatgg
    25981 ttatgatacc gccgttattg tccaagtcat cttcataatg gggatggttc ctgtttgagc
    26041 ctccccctat ccctgctccg taggcacctt gtccgacatt gtccccatat acttcacagc
    26101 tggcttcaac atagccgccg tttatagtta tatttccgcc gcttccttca ttgcctcccc
    26161 caataccggc cccatagccg ccgcttatcg ccctcacatg gcctccgctt attgtgatat
    26221 ttccaccgtc attgacctca tcttcaaagt cagtgcctcc accaatacct gctcctccaa
    26281 acctgcttat tgccgtaaca gtaccccctg taatagtaat gttccctccg gtggctccca
    26341 caccgccgcc gatacccgct ccctgtatgc cgccaattcc ctctacaact cctccgctta
    26401 ttgttatatt cccccctgag cctccaaaac caccgcctat gcccgctgaa tacatacctc
    26461 cggttgcttt aatcgttcca cccatgatat ttatagtgcc tgtttcctct gaagcgtctc
    26521 cgcctatacc tgcactatgc cacccattat tgacaacaat cagttgggca tactcatcat
    26581 tatcggtgct ttccttctta tcaactgtta gctgtacgcc ctcaggaaca tgtaagggtg
    26641 cagaattctc tcctgtcaga ttgcatatgc cgcttatata cagcttgttg tcatagcttc
    26701 cggcatttct taaatcaata acagatgata ggctcgctga catatccaaa tcctctatgg
    26761 taagctccag agcttccgtt cttccctctg ctatataaat ccttgtttct tcatgaggtg
    26821 tcttataaac tgaatctgta accgtgacct catataccgc atttgaaacg gtaataacac
    26881 cagaataacc ggaggaaagc tgatataccc cgttatttac tatagtatag tccccgtccg
    26941 gaattataac actgtcagcc tgcactgcga caatcccaag tgtcacagcc agcagaacca
    27001 caatacccag aatcaatccg atgcttttct tcatatactt tcctccctcg tactgttcat
    27061 cattactttt ataatacttt tttcaacatt tttatacaat gtactaaagt acagtctgag
    27121 aaaaaaagta cagtccaaat tgtaagactg tacttttgaa tatcaatttt tatccacaag
    27181 ccctatacgg gctgcataag ctataacctg agccctgttt tcaagatgca gctcctccag
    27241 tatcttcccc atgtgatatt ttactgtccg ttcagaaagt ccgagctttt cccctacctc
    27301 cttgtagata atgccctttg acaccagctc cagcacttcc agctgccgct ttgtgagttc
    27361 ctcattctgt atggtttttc ctgtatcccg gcttctgaat tccttcaaga gcttggcagc
    27421 gagctccggt gaaaaggtcg cttccccctt ttctatgtcc gacaatatac ctacaagttc
    27481 tttcgcattg gtattcttaa gcagatatcc cgaggcaccg cacttgacgg cataaaaaag
    27541 gtcatcatcc tcttccgagg tagtcagcat cactaccttt atgtccggca tttcggcctt
    27601 tatcagctta agggcatcca acccgctcaa acccggcatc tttatatcca ttaatatagt
    27661 gtccggtttc aatactctgg ctttttcaaa agcttctctc ccgtcttccg ctgtaccgac
    27721 tacctctacc ccataagttt caaggaggta ttgaagtcct tccataaaaa gctggtggtc
    27781 atcaaccagc agtaatttca ttttcatttt gcagttcctc cttattcaaa ggaataatta
    27841 aagaaatacg gcagcctcta cccggttctg attgaatacc aatctgcgca cctatttctg
    27901 atgcacgctc cctaatgatg tttaacccga acctggttgt attattgtta ttaagacaat
    27961 tctcaacatc aaatcctttt ccgtcatctt ccacagttac acataactgc cgctgttcaa
    28021 ttgcaaattc gaatttcaca ttattggctt cagaatgctt cctgatatta ttaatgactt
    28081 ctttaacaat attcaatata ttgatgcgta tgctcggtct tagttcatct cctgtaaatc
    28141 caaggggaat atttactttt acatttaatc ctgactgctc ctcaaagctt gcaatattcc
    28201 ttgtcagccc ggtcataaaa tccttttcca aagcttctgc attcctggca ttgcggatat
    28261 attcccttaa atcattatgg gcttcctggg aagcacgaac tagcttgtcc agcttatgag
    28321 atacaatgtc aatatctgca tttataagct cctgttctat tccctgggct tgaagattaa
    28381 taaatcccaa tacctgtcca aggttgtcat ggagatctct cgccagccgt tccctttctt
    28441 ctgtaacagc gtttttccac tgctgcttca ggtatctctc ctgctcctgc tttttttcag
    28501 ttatatcgta ggcaacaaca agccttccta tcattgctcc tttttgatct gcaagagggg
    28561 aaaagaatgc ctcgtaaacc ttcattcctt cattaatgcc tattacaaat tcactgtgag
    28621 ttatgttcct gtccttacag gtacatgcca gttccggaat tttacggcac actttctcta
    28681 ctgtctctgc gggagtctta acatccggat agccaattat tcttctcatg gccggattga
    28741 tatccagcac tcgatcctgc aaatccagca ctataacccc cgcatccata ttctcaatca
    28801 ccatggctcg tgccacgggt attacatcaa acaatctata ccggaatatt ccccaggtaa
    28861 taatcaatcc ggcaggccca aaaaaaagcg gtgtaatgtc atatctctct atcgggctta
    28921 gtcctgtgat atatagaaca tttggtatta caatcatact cagccccacc aatagtgctg
    28981 ttgcctgctt cttgtaaact gtgtttctga aaaagacggc ctttatcaaa agaatccatg
    29041 ccagaatatt cagcatgtgt gaatataagg catggatata gaaagcaggg ccatattttt
    29101 tatcaataac aggaaatgaa ccgctgtaat tcatatgcat attatatctt atcaagccat
    29161 gcagatgatc cgtccataca agaattacaa taatagtcgg aataacagca agccacagta
    29221 ctttccggtt tcggatccat ctgtcatatc ccgtaaactc catacacatt gcaagcaggg
    29281 tcagcggaga ataacagtat gcaaaatact gtacatttgc ccaaaaaagc tttgtagaaa
    29341 aatctatgct tgccatctca agggcattgc ccagggacca tatggtcaca acaagcatac
    29401 tcaatatgaa gcttacagcc ccttttgaat cccttcgtct cagtattaca aatattccca
    29461 gggataatga aataaatgca gatgtgataa ggggccatat atatggaata tattgtatcc
    29521 gcatttattt caactcctat acaaacctca gtgacacttc tcccagttcg gaaaaaacta
    29581 ccttaaaatc atcccctgct tctgcagcca gtgccgaagt cagagaaccg gataaaacca
    29641 cttctccttt tttcagtgtt actccatatt cccacagctt attaataagc catgccacag
    29701 aaagggcagg atctccaagt acatcagcgc caactccgga taataccttt tccccattct
    29761 tatataaacc cattgatacg gctttcaaat taatgctatt gggatcaacc tttttattcc
    29821 cgattacata cattgcagaa gatgcattat cggcaacagt atccacaatt cctattttcc
    29881 agtctttgat tctgctgtca accacctcaa ttgaggctaa tacatagtct gttgcttcca
    29941 taacatcttt tgccgttaca ttaggcccct gcaaatcctt tttcaataca aaggctattt
    30001 ctccctcaac cttcggatta agatatctgc ttctttccag aataccattc tttacttcca
    30061 taaaatcaaa cagttgaccg taatccggct gatccactcc aatgaattgc tgcattgcaa
    30121 gggaagtgag ccctattttc ttaccggtta tcttattccc tgatgcaaga agttcattaa
    30181 cattaatacg ctgtacttca taagcgtcct tgattttcag ttcgggatat gtctttgata
    30241 tgggctcaat cggtatacat gtcctttcag cttccttcaa agctgatgcg accttccgat
    30301 tcaagctgtc tttgctattc tccatcttta tcattccctt cagcctttga tctgcctgac
    30361 acagtccttt aaggcactga ctaaaatatt ggtatcaaga ctatatgcta ttccattaaa
    30421 accatccttg atatattttc ttgcagcttc caccgatccg ctgaatataa atatggattt
    30481 tccggcagcc ttgcaggcat tcagtatcct atcaaccgat tcggtaaaaa cgggattgct
    30541 gaactgtgca ggcatcccca aggctatgga taaatcatat gggccaacga atataccatc
    30601 cactccttca atggatacaa tctcctcaat gttatccagg catccctttg tctcacactg
    30661 gggtataatc atcgtctcat tattggctaa actaaaatac tcttctaaat ttcctgcagc
    30721 atgttcaaca aatccgtatc ctccgcacct tgtgggagca aagccccggc ggccgacggg
    30781 ataatacttg ccatactcca ccaatttttt tacctgttcc actgtttcta tgttgggaat
    30841 aatcagaccc ttagctccta catcaagcat cttcagtaca gaagaacgtg tatgatcctt
    30901 tatccgcaca aaaggttcaa tccccctaag ttccgctgcg cgtataaaat ccatggcgct
    30961 ttccacatca aagggaccgt gctctgtgtc gattataaga aaatccattc cggaaaggcc
    31021 gatgcactct acggcagcag catcacccaa ttcaaagaat gtaccgatta caggcaatcc
    31081 actttccatt ttgcttttta cttcgttttt cattgctaac ctcctgcata tgctgaatat
    31141 cgttttctat acagtccaat tataacataa aagaaaatct ttattcctgc agcaccatga
    31201 aaatttccct gtacaatatt aagtcaaaat atgtaaaagc agcttgatta ctacaggata
    31261 aaaggcaata atactgacaa ctcttgcgaa ttgcagcaca gcaacttttg gagcatctgc
    31321 ccccatttcg ccagcaatta tagccatatc ggtaagccct cctgctgttg aggaatacat
    31381 ggcagtggtc acatccaaag tactgaactt tataagtaaa aatcccgata caaaagtaag
    31441 cagtatatat cctgcgataa ttataatcaa agccggcagc atccctctca tacccataac
    31501 ctgttccatt gttatcctgg cacctattag agctcctccc agaaattgaa tgccctttct
    31561 taaatttata ggcatatatg ctcttgaagt tcttatatta tacacagcgc aagctatcat
    31621 ggaaaagctg atagacccgg ccgggaaacc cgtgacatgc ccgataaatc caaaaacaaa
    31681 ccctacggtc atcgttatta atgtcctctt gaatatttca ttataatccg cacaggactc
    31741 ttcatatttt tcgggcggtt tgtcttcaat ctgagcttta tttgattttt ttaagtagta
    31801 ttttattaga ttcggcataa tagcaattac cgaaacaagc ctcacaagct gcactgctgc
    31861 aacctttgaa gaatcagcac caaaatccac tgaaataagc gtcatatctg ctatacctgc
    31921 cggagctgtt gcaaaaagtg ctgtcgtcaa atctatgtct gagatgatac ttagcagggt
    31981 acccgcaaat atgccgaaaa ggcacataga aacagttaat attattgcag gcttgattat
    32041 actctttatc tcagcaacat ctttattgca tattcgtgca ccaatatatg ctccggttcc
    32101 tatttgagtc accagcttaa agctttgagg aaattcagct tttccggtga aaatactgaa
    32161 cagtgctaca gaaattagcg aaccgattat tgctcctgcc ggcatcttca gtctgacagc
    32221 tgtcaaaccg ccgataatgc caattataat tgttatagcg atgaacataa aaaagcctcc
    32281 ttgtaggagt cgtcttgacc gcatatgctt atagtagcat attttcaatt ttcagtcata
    32341 ttttgttttt ccattgtgcg cttattatat atgaaaaaga acgttctccc ttaatataat
    32401 aaaaattcct gcctgtttcg gacttttccg cagcatcagg caggaaccaa tcattgttaa
    32461 ccctattgca cctaaaccat tattttacaa atattattcg tgaattctac cggatcgcct
    32521 atgggcaaac cttctatcag aagggcttga ttatataata gattcgtgta caggttcagc
    32581 ttctctttat ccttttcaaa ggcttcctta agggatttga agacttcatg atcggtattt
    32641 atctccagaa tcttatcagc tttgataccc ggactgttcg gcattgaatt aagcaccttc
    32701 tccatttcaa tagaaagctc tccttcgttg gacaggcata cgggatggtt tttcagcctc
    32761 actgatgctc tgacatcctt gacctttccc gacagtatgc ttttcatatg ctcaaaaagt
    32821 tccttactgt ctttctcctc agactcggat aagtttttct tctcttcggc ttctataccc
    32881 aaatcactgc ctgataccga tctgaactct ttgtccttat atgtcatcaa catttttatt
    32941 gaaaattcgt cgacatcatc ggtaaaatac agtatttcgt agcccttgtc agataccagt
    33001 tctgcctggg gcagtttttc aatcctttca ttggattctc cggtggcata atagatatat
    33061 ctttgttctt cggacattct ggaaacgtat tcatccaaag tgaccatctt tttctccttt
    33121 gatgaataaa acatcaacag gtcctgaagg gtttccttgt tgcttccaaa ttcgctgtat
    33181 attccgtatt tcagctgcct tccgaaggct ttgtagaatt cctcgtactt atccctctcg
    33241 gttttcaaca gattctccag ttcgctcttg attttgtttt ttatattctt ggcaataagc
    33301 ttcagctgcc tgtcatgctg gagaatttcc ctggatatgt taagggacaa atcctccgag
    33361 tccgcaaggc ctttaacaaa gctgaaataa tccggaagca ggtcagggca cttgttcatt
    33421 atcataacac cctttgagta aagctccagg cctttttcat attcctttgt atagaaatca
    33481 aagggcatct tttccggaat gaacagtatt gcattatagc tcacagcgcc gtcaacactt
    33541 atatgaatat gctttaaagg tttgtcaaag ccgtagtgct tttccgaata gaagttatca
    33601 taatcttcct tggtcagttc gttcttgttc cgtttccata tgggcaccat actgtttata
    33661 atctgctctt ccttatagcc ctcgtattcc ttgtcgctgc cttccttgag acgtttgctg
    33721 accatatcca tttttatcgg atacctgata aagtcggaat atttctttat aatagcctta
    33781 agcctgtact cttcaagata ctcgtcatat ttctcatctt cggtattctc ttttatcttt
    33841 agtatgatat ccgtaccgac agtatctttc tcacaaagtt caatcgtata accgtctgcc
    33901 ccgctggatt gccacttgta cgcatcatca cttccgtagg ccttgcttat taccgtaaca
    33961 gtatcagata ccataaatgc cgaatagaat cccactccga actggcctat tatgtcatgc
    34021 ccgtccttta gttcaatttc cttcttaaat gaaagagaac cgctttttgc tattactcca
    34081 agattatcat ccagttcttc ttttgtcatt ccgattcctg tatccgaaat cctgagggtt
    34141 ctgttttcct tgtcaggtac aacctttata taataactgt ctttatcaaa gcttatagat
    34201 tcgtccgtta atgctttgta ataaatcttg tcaatggcat cacttgcatt ggaaataagc
    34261 tccctgagaa aaatctccct gtgagtgtaa attgaattga tcatgagatc caatagcttc
    34321 ttggattctg ttttgaattg ttttttagcc ataaaaattc tcctcccaaa gaaaatgtca
    34381 tatgtattgt tagcactctg cctatttgag tgctaaccct ctatccatta tttatcacaa
    34441 ttcaatttct atgtcaaggc ttattatgta aaagaagaag aatacgacgg gggacacggg
    34501 gaggacacgg agacgtttct cttgtcccgc tttcagcggg acaagagaaa cgtccccgtg
    34561 tcctccccgt gccttttcgc cgcattcttc taattgaatt tgtatatcct gcgcatgagc
    34621 cggtataatt gaattgtaat ctttcttcca accttgccgt aaggattgga taatgttcct
    34681 aagaagcggt atctcagcct gtaatagagc atttcattgc tctttttaag atatgaccac
    34741 aattccgctt tcttctgcaa atcctctttt gtggcggaga tctgaagaaa caccgatgaa
    34801 attgtcatca tcatggaaag ataacttctc atataatttg caagccgtct gctgggcaga
    34861 taatcatagg aattataggc atctatcata agcttgttta ctcgaatttg ctgatcaatt
    34921 cgctttatca tgtttttttc atttaccgat tgatcttctc tcccgataaa atagcgatac
    34981 agatcaatat tcatataata aatggttctt acataaggaa gcgggttgta cacgaatagg
    35041 ttgtccacat aaaaagtatg cttgggcagt accaggccgc attgccgaag aagctcggta
    35101 cgataaataa ccgaatgcat gagaaggtat tgagtcggtt tgaagttacc tatctcatcc
    35161 cagccgaaaa cacggttctg gggcaataca tacttgtaat tgacaacatg actgctattg
    35221 tctgatgaat gctcataaac ataattacat accatcatat cgagagcagt acctttttca
    35281 tgcagctcct taagcttgga cagcactttc agcataccgt cggcatccag ccaatcatcc
    35341 gaatccacaa ccttgtaata caaaccggcg gcatttctca aaccggtatt gacaccctcg
    35401 ccatgtcctc cgttttcctg atgtatcgct ttaacaatgc cgggataccg ggaagcataa
    35461 tcatccgcaa tttcacccgt tccatccgtt gaaccgtcat ttacaatgat tatttcaaca
    35521 tcttctcctc cgagaagcaa agattctatg caattcttca tatatgcctc ggaattatag
    35581 caaggaactg taaaagttat tatcttcata tcccactctc tttcccgcat tatatgatgc
    35641 tgtcggccag cgacaatgcc cgctctgcta ttacatccat gttgtagtat ttgtattcag
    35701 ccagcctgcc aagcagataa aaatcatcaa tagcctcagt aatctgttta taatttccat
    35761 ataatgcttc attctccgga tttattatag catagtaggg tatttctttt ccagcaccag
    35821 tatattccct gggatattct ttcatgatag tggttttatc cggtattatc tgacctgtca
    35881 gatgcttgaa ctccgttatg cgggtataag gttcgtcaac tgtatagttt acagttccct
    35941 tagactgata ccaggtcttt tcatgagttt caaacaaaaa atccagagtt ctgtatggca
    36001 gccggccgaa gcggcaatca aaaagctcgt ctatagcacc ggtatatatt actctgccgt
    36061 aaaacgggct gccgcaaaaa tatatcctgt tttcaaccag cttaagcacg gaagctgaat
    36121 cagtgttaag ctgcaggctt atgttcggat gatcaatcag cctttcaaaa agggaagtgt
    36181 agccgtttaa tggaatgccc tgatattcat cctgaaaata ccgattgtca taggaaataa
    36241 gaatgggaac ccttgccgtc actgaaggat ctatgttttc cggagcaata ccccattgtt
    36301 tttgtgtgta atacatgaaa acatttttgt atataaagtc tgcaagttca agcagttcgc
    36361 tgtcggtctg ccctctcagt tcggatattg tcacccgctt ttccgttcca taaaccgaaa
    36421 tgagcttttg ctcaagcctt tcagctttgc tttgttcaaa aaccagataa agagaattga
    36481 gattgaaggg aaccggaacc agttttccgt gaatatcggc cagaacctcg tgagagtaat
    36541 ccctccactc tgtgaaacgg gaaaggtatt caaatacacg gctgctttga gtatggaaaa
    36601 tatgagggcc gtaattatga attagtattc cgtcatcgtt gtatgaatca taagcgttgc
    36661 cgccaatatg gctgcgctta tcaataatca aaaccttttt gcctcccctt tccgcgtgtt
    36721 ccctggctat caccgctcct gcaaagccac agcctatgac aaggcaatca aattcatttt
    36781 tcatttgccg gaattacctc catttattta tatacttcta tcaatgactc atataaatac
    36841 acatattttc atcagtatcc tttatcaatg tccactatat taagaagcgc ttccccattt
    36901 atataccttc tgagattttc ataaactata ttatatcttc tttcggttct catttcggat
    36961 atccaggaat tatggggtgt aataataaca ttgttcatct cccataaagg gctttcctca
    37021 ggcaaaggct ccttctcaaa cacatccagc gccgctccgt gaatatttcc cttccgaagc
    37081 gcatctataa gcgcaggctc ttttataatg cttcctcttg caatgtttac caggcaggca
    37141 ccctttttaa taaaactgat gctttcctca ttaattaagt ggtgagtctc ctttgtatac
    37201 ggaattgaaa gcaccactac gtcagcttgg ggcaaaatgt tatgtagttc attcattggg
    37261 tggcagctgt tgaaatactc cactttcctg cctgtggagt ttattcccaa aatatttaca
    37321 tcaaatccct gaagcctttt tgccgcctca agagctatac tgcctgtacc gatgaaacag
    37381 acagttttat tgtaaagctc taacagtgtt gaatccggtt gccacttttt ttgtctctga
    37441 ttatcgtaaa atcccttgct gttttttaag agtgcaagaa ttttcataac aatccattca
    37501 cccatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacct caaatttttc
    37621 aacttaaccg agttttcaca tataaaaagc cttcatttat cgtaaatgca ggatgtatat
    37681 attatacatc aaattattgc tatatagaat acagtattat ttattatctt caaagacaca
    37741 tagcggggga catctatccg ccacttacta taaagaaaaa aaggagtgtt tctcacaccc
    37801 cttatccatc ctgatatgaa tctgattatc ggaatttccc aagacaaata ctatgcagac
    37861 tctttgtcct attaatactg aattttgcgt ggaagattgc cagcctgctc aatccacttt
    37921 actatctgtt tcttgaaagg cattgccttg attagctttt tttcttggtt tatactttcc
    37981 atctttgcca agagattgtc cggcttacga tttgccaatt ccggtacagt atcaacgccg
    38041 gcagcttcca gtagttctga gtactggcct ccgattccct ttatcctgaa aagatcggca
    38101 tgattagtcc acttcaatat caatttttcg ctaatacctg tcttttcagc taattctgac
    38161 ctgctctttt tggttagaca ggcttccaac agctcttcta cagaattgat acccgcgtct
    38221 ttgagtttct cattataggc ttcacctatt ccctctatca tactgagctt tttcattata
    38281 agatcctcct ttttaatatt taatttgtat acatatttgc atacctattt tttgaataac
    38341 ccaccaagca agtcgccaat attatccatt acatctccgt cattatcaga atccaacaaa
    38401 ttagtcacaa tgcccattaa attgttgtcg ccgccgtcca tcaaaccgcc taacaaacca
    38461 acaactccgg aaacatccaa attctcttcc tttttctttt ttcccaatgc acccatcata
    38521 agtggtgcca actgggtcat aattccagat acttgacttg tattgagtcc ggtttgcctt
    38581 gccaaatttt cctggactct tttattattt cctgaaaata tatgctgtaa aatctttgct
    38641 ccgtcgttgg tatcaacttt atttaagaaa ccggttaaat cttcaatttc gtccttctga
    38701 tgcccatcca aagctcccaa taaagcttct gcaccttctt ttgtgctggc attgcgtcca
    38761 agagcttgca atatagccgg tataccaagc tgtgtcagtt tctgtgcctg tgaggactca
    38821 actccaacag atccggcaag ttttttcagt gtatcagggc tattaagctg attagtaagc
    38881 agtttaacaa tatccataat actcctcctc taaatattcc ttgaatttac ttaattatat
    38941 cataggatag tatttattta aatgacttat ttattaataa ctatttttgt ctataattac
    39001 aataaaacac atttactgta aaaccattaa tgagctatat ttactatatg taaacatagc
    39061 tcatcaatgg tttttgtaat tattcagata ttgaaagccg tattatgaca attgctactg
    39121 atgctcttga tgaatactag tgtcattctt tttataggat aagctccttt atcgttatgc
    39181 cttcttttat ttccggaacc ctaattctat tatctactgc ctttttaatt ttcataggca
    39241 gattagaatc tccgaacaca atagcaccga taattatact atcctttatc aaaagcttta
    39301 tgtatcttcc gctttcaaaa tcagcttctg caatcgaaag agaatcttcg gaaaacacat
    39361 ctccgattga aaacattgtt atccccttta tcttaaggct tgtaaaggga tgaatctccg
    39421 aatactctaa tttccctccc gctgcatttg ttccggcaat tttaccctgt tcaaccgcta
    39481 taggccatat gccatagcat ttgccgttat attcagctac atcacctgcc gcatatattc
    39541 cttcaatatt tgtttccata tatttatcaa ctgctacaga cctgcccata tttatccctg
    39601 agtctttcaa aggttgtgta tttgcattga ctcccacaga caatatgaca aaatcggatt
    39661 tgactactgt tccgctgtca agcctgacat actccgccct gttttctccc ccgatttcct
    39721 gcacctgtgc gttcatatac agattaatac ctttattaga tattgccttg tataatagtt
    39781 ttgctccttc ctcatcaatc tgctttggaa gcagcctgcc tgaattatgt ataacattga
    39841 catcacagcc aagctgcctt attgcccaag ccatttcaag ccccaaaagc cctcccccta
    39901 ttattgtgca tatttttccg ccgcaggaca agcttcttat cttatcagca tcagccacag
    39961 tacgcacagg aaaacagttt tccagttttg ctcccggaat aggaggaata ttgccgctgc
    40021 tccctagtgc aataaccagc gaatcaaaat cataattccc cttatctgtt attatggttt
    40081 tatgtgcagc atcaattcct gtaatcctgg tgtccttttt aaggtttatc ctgttctttt
    40141 cataccagct gtcatcataa gggaatattt ctttgtatga agcagtaccg gatatatagt
    40201 cgggaagtct ggttcttaca tatgtatttg tattcaaagc ctccagtata acaatactgc
    40261 agtccggatc ctgttccctt gctgactttg cagcattcaa tcctgcaatt ccgccgccgg
    40321 cgattactat ccttctatcc atattaaaac tcctttcatt aataccataa ttgaccatta
    40381 ttcatattta tcttatctta atatatttcc attttcccgt tttaaacctg aaataaacaa
    40441 acatccatct gacaagctga tctaccaata cgcctatcca cgcccccatc aggccgtagc
    40501 ccattacact gacaaatata tacgctaaca caactcttac catcaataca ccaacagaag
    40561 tggataccag aggccagaag gtatccccag cacccctgag ggcccctgcc aatatgaatt
    40621 gagatgactg gaagggctga accagtgcaa ttatcttcaa tgcaacggaa gaattcttga
    40681 taatcatcgg atcattggaa tataacccta caagctgcgg tccgaaaaag aaaaatatta
    40741 tagccataaa tgtagaaata attgacccca tctttcgggt ttttctggcg tattcttccg
    40801 ccatatcagg tttccctgca cccagggata tccctaccaa agatgacgct gctattccaa
    40861 aggcctgtcc cggttgaaag gaaagtccca atatactcag agcaatctga tgggctgcat
    40921 aaataacggt ccccaattca gcaactatcc tgacaaaaag aagtacacct gtccttaaga
    40981 ccaattgctc aaaggatgcc ggcataccta ttttgaataa attatataaa attctcctgt
    41041 ttagattaaa gggcttcttg atgctgaatc ttacaacgct cttacctttt aagacatatc
    41101 cgaatataaa tacacttgca accgcatttg ataatgctgt agagatgcct gctcccgtaa
    41161 tcccaagtct tggaaatccc agcaagccgt atattaatat tgcattgcct ataacattta
    41221 agaaattgca tctcaagttt attatcatcg gcactcttgt ttcgcctatt cccctcagtg
    41281 ccgctgatat ggacatatta agagactgaa atacatatcc gaccattatt atcctgaaat
    41341 agattctccc cacttgcagg gtatctgcct gggcacccat caatttcata atggaatctg
    41401 taaagacaaa ccccaatata gacagaggta ttgccagcat cagcatgctt agaagcatta
    41461 catgcttaag ggttatttct atcttctcat tctgattggc gccgaaatac ctggcaatca
    41521 ttgctgtccc gccgatattc agagcctgaa ctaaagatat acctataaac agcggttgat
    41581 ttgtcatgcc aacagcagct acggaagcag ctgccaaagc attatcactg attcttccca
    41641 gcatcatcat gtctaccatt ccgaataatg accccaaaag aagctctgcc agtaccggcc
    41701 atgcaatcct cataacttcg gcatcggcct tgccctttat gttcaagaag tttttccgtt
    41761 ctcttttgtc attctctgct tcgttcattt taatgattcc tttttaaata tcaatattaa
    41821 tactattata tcataaatcc gcaaggattt atgatagccc atgtatcaag ataaaagcag
    41881 ccggaacaat ccccactgct ttccgcaatc atcagttgca gtcgttatat gctttataaa
    41941 acagttttac atattcatcc ttgcttatct tccttggatt actgtcatta gagccattct
    42001 tttcggacat ttccgccaac agctcgaaat catcggtatt gacatttaaa gaccttattc
    42061 ccggcagccc tatttccttt gacagctttc ttatatgctc gatacctttc actgccgccg
    42121 tgtaatcatc tttttcttct atacccattg cccttgctac cctggcatat ttatgccctg
    42181 cggcaggcag gttatattcc atcacatatg gaagcaatat ggcattgcac attccatggg
    42241 gcgcatcata catgcttccc agtgcttctg ccatgcagtg tactgaagct acatcggcat
    42301 gactgaaaga taagcctgcc agcaggcttc caatcatcat tttgtctctt gcggctatat
    42361 ttctcccgtt cttaaccgct tcttcaatat tgcctgctat gtattcaact gcatacaagc
    42421 ctgctgcttc tgcaataggt tcggtacaat ttgccgtgta accttctata gcatgagtta
    42481 aggcatctat acccgttgca gctgttatca gcggcggtac gcttaatgtc agctcaggat
    42541 ctattatggc tgttttggct gctattgccg tactttttat tgtaaatttg aatttttcct
    42601 tagtatccgt aataactgaa gaaaaggtta cctcacttcc cgtaccggct gtggtaggta
    42661 tagttatcaa aggcaaacag tcatctttta ttctaccctt accctgataa tccctaacct
    42721 ttcctccttg tcttgctaaa actgccgttg ctttagccgc atctataggg cttccgccgc
    42781 cgaaagccaa cagcgtatca actgattcag ccctggcttt ttcagcacat gcttctacat
    42841 tataatcctt tggattagct tcgatttcat cgtaaactat aaaatctata tcttcagctt
    42901 cgattatctc tgcagccttt tttatcattc ccgcattaac aaggccttta tcggttatta
    42961 tcattacttt ttttgctttc aataatctca attcttctcc caatactttc agcatacctg
    43021 ccccatacct tatcttagtc ggcaatacat attcaaacgg atttatcata tttattccct
    43081 ccttaattca ttaaccaatt attcctttcc gttccagcag tacgtacaga ccttatcctt
    43141 gctgattccg attgcttcaa tcatatcgtg gatgttctgg tacttcagcg aagaaaagtt
    43201 gagcttcctg cagattagat ccaccatatt atgatacttg tcggttgcct gattgcaata
    43261 ctcctccaga gaatccggtt ccttgccttc aagttctttg atagcctgtc ttgccgccag
    43321 gtccatttca gaccgggagg ccgaaaagtt caggtacttg catccgaaaa caataggcgg
    43381 gcaagccgac cggatatgga cctccttggc gctgcatttg tacagcaggt ctaccgtttc
    43441 tctcaactgt gtgccccgga caattgaatc atcacagaaa agcaatcgtt tgtcagccac
    43501 aatttcagga atgggcataa gcttcattct tgcaacaagg tttcttatat cctgatcctg
    43561 gggcataaag ctccttgacc aggttggggt atactttata aaaggccttc cgtagggtac
    43621 cttggattgg tttgagtagc cgatggcatg tccgaggccc gaatctggta ttccggcaac
    43681 tatatcaatt cccaaatcct catttttagc cattgccgca ccgtttctgt atcgcatgac
    43741 ctcaacattc attccctcat atgttgaaga aggataaccg taataaaccc aaagaaaagc
    43801 acatattttc attttatctc cgggaggtga aacctgttca accacctccg gtgtcagaaa
    43861 aactatttct cccgggccga gttcataatc agacttatat cccagatttg caaaggtgca
    43921 ggattcagaa gaaatacaat aggcgccctc tttgctgccg accattaaag gagttctgcc
    43981 aagcctgtcc ctggaggcat aaatcccttt aggcgttaaa agcaaaacag aacaggagcc
    44041 ttgaatcaat tcctgagccc ttaataagcc ctctttaaag gaatcttcat gatcaatgat
    44101 tacagacaca agctccgtag gacttattat ccctttattc gtttccagaa aatgaatatt
    44161 tcggtttgag aatgcttttt ccgcaatttc atccaaattg ttgattcttc ctacagtact
    44221 tatggcatac tgtcccagat gcgatctcac tgtcaaaggc tgagggtcgg tatcgcttat
    44281 gcagcctata cccatgttgc cgcttttttt tgacagttca gcctcaaatt ttgctctaaa
    44341 ctgcgtactt tctatattgt gaatcgatct gttgaagcag cttccgcccc atattgccat
    44401 tcctcccctg caggttccca aatgtgaatg gtaatcggtg ccgaaataca catccatcac
    44461 gcaatcttct cttgaaacaa caccaaataa tccgcccatt gcagctgcac tccctttaac
    44521 atattttacc gcatatactg ttagatttgt cggaaaagac cggtatatac attaatatag
    44581 ttgcattttt attataattt tgcaagaata ttttatttcc aaaaaataaa cagcacccca
    44641 ttcgtctata gaataaggtg ctgtatatgt aagttgttcc tttcagcgtt cttccctgaa
    44701 atatggaatc ccgcatgcct tcgggcctgc cttttccctt gacttccgca tggataccac
    44761 tacaagcacg gtaattgtca ccaggtacgg cagcatgtct atgaaatact gggatacatc
    44821 gatacccagg ttttgtattc tgaaaccgat gatatccaat ccgccgaaca gataagcccc
    44881 aaagagcgcc ttataaggat tccatgttgc aaatatgacc aatgcaacgg cgatccagcc
    44941 tctgcctgct gtaacgccct cctgcagggc gggtacataa acaagtgaaa ggtaagctcc
    45001 tcccaggccg cagagcgctc cgcccaataa agtatgcagg tacttgtaca aggttacatt
    45061 tattcctgca gcatctgctg ctcccgggtt ctcacccacc gccctcaggt tcatccctgt
    45121 actggtctta tacaggtaca gccccagcag tatagatgcc gcatagccaa ggtatacaaa
    45181 ggcatcctgc ctgaagagca caggccctat aaaaggaata tcgccaagca gcgggatggt
    45241 gtaaggcctg aaaaacaatc tcaagctttc aggagctatc tgacctatca gatcctgacc
    45301 cacaaaatcc gcaaagcctg tgccgaatat cgtaagtgtc aagccggata caacctgatt
    45361 tgccctcagg gtgattgtca ggaaagcgaa tatcaacgcg ccgcccgctc ccgcaatcat
    45421 tgctgccaat aaagccaagg tcgtgctttc ggtgtttagg ccgaccatga agccgataac
    45481 ggcacccata agcatcatgc cttcaactcc aaggttcaga tttcccgaac gctctgttat
    45541 gatttctccc agcgtagcaa ataaaagggg tgtacctgcg acaatggccg catggagaaa
    45601 ggatataaca gcttccataa tcaatcagcc cccttttccg caatagccga atgctttttt
    45661 acaattacga acttataacg tacaaagaat tcactgccca atacaaagaa taaaattatt
    45721 ccctgaagta tttctgccgc tgcctggggt atgccgaaaa cagtctgtat atatgagccc
    45781 ccctccagaa gagcagcaaa aagaatggat actatcaata taagcggtgc acttaaagct
    45841 gccagccaag cggttattat tgcggtataa cctacacctc cggtaatttc aactgacaat
    45901 gtgtagttaa cagccgaagc ctggatcata ccgacaaggc cgcagaggcc gccgctcagc
    45961 attatggcta tcaatatcgt cttcttgata ttaatgcctg cataacgtgc cgtattcatg
    46021 ctctcaccga ttacggcaat ttcataaccc ttttttgtat ggtttatgaa tatatagaca
    46081 acgaccacca gtataagcgc aatcacccag cccacatgta cccccagcag cttgggaaga
    46141 accgcgtttt ggggaaaatt ggctattttg gggaatccca gggactttgg atccctccaa
    46201 ggcccgtact gtaagaacat gacccattta agcgcgatat agttcatcat taaagtaact
    46261 attgtttcat tagtccccca ctgcgcttta aaaaatgcgg gtataagcgc ccacagccct
    46321 cctgccagca ttcctgcaag tatcattacc gtaagcatca gaggcattgg catatcattc
    46381 atgtataatg caaaataact ggctgcgaaa gaacctattg ctatctgccc ttccccgcct
    46441 atattccaga actgcatttt aaaagctatc gcaattccaa gggaggtaat taccagaggt
    46501 atcgctttta ctattgtttc ttttatcctg taagctgtac cgaatgcgcc ctgaatcatt
    46561 tttatataaa catcggcggg attaaggctc agcagcagta taaaaagtga agaggccacg
    46621 agagccagga taatagcgcc tattctgatg gaagcagctt tcatcctgcc gggctcgctt
    46681 ttcttaatgg ctcgtatagt tattggcatt atcatgctcg ctcttttccc cctctttcat
    46741 cagatttacc gtccctgtca tcatcaggcc gatttgttct tttgtcgtat atcttgcatc
    46801 aactattccg gttatttcac cggcacatat gaccatgatc ctgtcgcaaa gctccagtaa
    46861 gacatccagg tcttccccta tatacagaat tgcgacaccc ttccgctttt cctcattgag
    46921 aaggtcatat atggtatagc aggagtttac atcaagcccc cttacaggat atgcagttat
    46981 cagaacctgc ggccgggagt ccagctctct tcccaataat accttttgta tatttcctcc
    47041 tgagagcatg cgcaccggat gcttgattcc cggagtctga atactgagcc tttctataat
    47101 ctccacggac ttttgttctg ccggctttct gtctatgaag atgcccttct gtttctgata
    47161 cagcttaagc acgacattat ttacgatatc cattgaggca accagtccca ttcccaatcg
    47221 gtcctcggga ataaagctca ggctgacccc cttggttatt atctctgcag gtgatttgcc
    47281 taccaggttt tctccattga aaacaatctc cccctgcttc acgggatgca agcctgcaat
    47341 agcttcacac aattccttct gcccgctgcc ggcaattccc gcaacaccca gaatctcacc
    47401 ggagtatata tcgaagctca tccctttaag cactttgatg ccttctgaat ttacagcgct
    47461 taaattcctt acttccatag ccacattgtt tttctcagtt tctatcctct ttatggacag
    47521 gtcggcagga catcccatca tcagctcggt aaggtgcttg gggttggtat ccgccttgtc
    47581 aaccgtcatt actgtttcac ccttgcgcag gacagttatg cggtctgcta cctccataac
    47641 ctcgttcaat ttgtgagtta taaagattac ggcgcagcct tcttccttca tcttcctcat
    47701 aatcttgaaa agcttttctg tttcctgagg tgtcaatacc gccgtgggtt catccaatat
    47761 cagtatctca gcacctctgt acagcacctt cagtatttcc agggtctgct tttcacccac
    47821 cgacatgttg taaacctttt tgtccgggtc aatatccagg ccgaagcggg ccgacatatc
    47881 cctgatcttg tctgaaagag tcttttcgtt catcagaaaa ctgttctttc gccccaggat
    47941 tatgttctct ttggcagtca tgacatctat cagcttgaaa tgctggtaga tcattcctat
    48001 tcctgcttct atcgcatcct ttggtgctgc aaactgcacc tctttcccct taataaaaat
    48061 agaaccgctg tcgggggtat aaacccctga cagcatgttc atcaatgtgc tcttacctga
    48121 accgttctct cccaacaggg catgtatctc ccccctgcgg acggccaggt ttatatttct
    48181 gtttgcaact acctttccga aggatttatt tatattcttc attgaaatat agttgttatc
    48241 cataattatt tcctatcctg acttatttaa tcttgccttc tacaccctgt acaaaccagt
    48301 caacgctcat tatttcatca aaagtcattg acttgccttc cggtaccctg acagcgccag
    48361 tctgatcctt tatcgggccc tggaatacat agaatgaacc atcgataatc tttgctttca
    48421 ccttgtcaac agcttcctga gtgcccggag caacgttatc cgtaagaggt gccaggtcaa
    48481 caagcccgtc tttcatattg ccgtagtagt tttcctgctt ccatttgccg tccaatatcc
    48541 tctgtacttc tgccgcatag aacttacccc agttccatac gggagcagtc aggtatgctt
    48601 tcggtgctga atcccttgta tccaggtcat agccgatagc ccaaacgcct ctttcttcag
    48661 ctgcctgctg ggttgctgtt gtatcctgat gctgtgcaag tacgtcgcag cctgcatcca
    48721 ataaagcctt tgccgcttcc ttttcctttg cggggtcata ccaggtatgt gtccagttaa
    48781 cctttacctt tacttttgga ttgactgact gtgcaccaag agtaaatgca ttgatgcctc
    48841 ttacaacctc gggaatttca aaggctgcta cataaccaag ggtattgttc tgtgtcctga
    48901 ggcccgcaac tatacccgca aggtatcttg cctcttccat ggcaccgaaa taattgctga
    48961 aattcgggcc actcttatag ccggagcagt gatagagttt tatgtccgga tgcgcctgtg
    49021 cggctttttc tgtatagtcc atgtgtccga aagagttggt gaatattaca gtacaaccct
    49081 ggtcaaccaa atcattgatt accttttcgc attcgctgga ttccggaaca tcttccttgt
    49141 acattgtaat aaccttgtct ccaagctgct gttcaaggta aagcctgcct tcatcatgag
    49201 cctgggtata tccgccgtct ttagcggagc ctatgtagac aaaacctacc ttaaggggtt
    49261 cagcctttgg ctcttctgcc ggtgcaggtg tctcgggcgc tgccggtgcg ggttcggcct
    49321 tcggtgcgca gcctgtcagc attgctgcaa acaccaggac aagcactaaa agaatactaa
    49381 tggttctttt catactgatt tctcctcccc aaatttaatt tagtggtcta tgtaaaatca
    49441 tgaaactggt ggaatcataa ttttgctttt gttaaccgtg ctaatcaaaa atgatgactc
    49501 cttcgtccat ggacttaata attaccaagg attcaatacg gactcctttt tgccgcagcg
    49561 tctccctgcc tttttggaaa cctttttcta taacaattcc gatgcccgca accctggcac
    49621 cagcctgaag gacaatgtcc accaagcctg aagcggcttt gccgttagcc aaaaaatcat
    49681 caatgataag aacattgtcc tccggagtga tgtatttttt ggaaacgcgg atgttgtagt
    49741 tcctctgctt ggtaaaagag tatacttcgc tttggtaagt atccgaatca agattcaatg
    49801 cttcatactt ttttgcaaaa accacaggca ctttaaaata ctgggcagct atgcacgcaa
    49861 cagcaatacc cgatgcctcc actgtcaata tcctgcttat cttttcatcg ctgaatcttt
    49921 ctttaaattc ccgtcctatc tcgttgaaca gctctatatc cagctgatgg ttcagaaagc
    49981 tgtcgacttt aataatgctg tcgcctatga cttttccgtc tttcaatatt ctatccttaa
    50041 gcagctccat tattatccct ttctatcacg gctgtttctc gataatgtaa tagcatatta
    50101 cctgaatcgg tgcaaaatac cagtacatta cacattgtat tttaccgtaa atgcctgttt
    50161 aatgcaataa gctattgctt aacaatcaac atccagagcc cttttcactg cattgaatat
    50221 tttgttgtaa cctgtgcagc ggcataaatg acctgaaatc tcacgtttta tctcctcatc
    50281 ggaatatttc cttccgcttt ccgtaagagc agttgccgta agcacaaggc cgggagtgca
    50341 aaaaccgcac tgcacggcac cctcgtctac aaaagcctgc tgcactttcg acaattcacc
    50401 ctccttggat actccttcaa tagtagttat tttcctgccg tcagcccata ccgccaggta
    50461 aatgcacgaa ttcaccggaa acccgtcaat cagcactgta catgctccgc attcgcctac
    50521 ggagcagccc tgcttgactc cggtaagatg aagcctgttt ctcagcacct caagcaacga
    50581 ttctctaata tcaacctcaa gggaatatgc cttaccgtta accaccatac ttatcttttc
    50641 atacttatcc aatatccaca cccccgcccc taatcacagc ttctttcagt gccctgaata
    50701 ccaatacctc aatcaaatgc tccctgtaag cctttgaagc cctccaggag tcccttgctc
    50761 ttgatgcctt taccgcatat cttccggttt ccttcatggt tttatcggat atttccttcc
    50821 caatggcata agcttcagct tcactgcacc ttatcggtac aggggccgca actccgagag
    50881 ctatccttac atcagaaaac ttacccccct cagttttgca tacgacagat acccccagca
    50941 ttgctatatc cattgccttt ctgccgctgt attttatata gcagccgctg gtccggccat
    51001 atcctgcctc gggtatcaaa atgtcggtaa gtatttcagc aggaagcaga ttcacttttc
    51061 ccggccccgc ataaaactcg cttactggta ctgttcttga cccgctgcgg cttaaaagct
    51121 tcagttccgc attataggca agcagcgtag ccgcactgtc tgccgacaca gcaccattgc
    51181 atatattccc ccctatcgta gccatatttc ttatctgcgg gcctcctacc gatactgccg
    51241 cttccgacaa aaccggcaaa ttctcgttta ttatgctgct cctggctatt ttggcaaagg
    51301 ccgccattgc cccgatggaa atggttccgt tgtcaagaat tcttatttca tccatcccgg
    51361 ggatatttct caggcttaga agctcagctt cctcaagagc accgttacgc atcttgatca
    51421 gtacatccgt cccccctgct atgattttca gttcaggatt tcgggaaagc atttccaaag
    51481 ctgcctccag agtgccgggt ttttcaatat tgacaatatc aaacattcct atctctcctt
    51541 tctaaagcag ccctgattgt ttaaactttt cgaaaacctt ctggggcgtc ataggcagct
    51601 tgttgaatgc aatgcctgtg gcattcagta ctgcattcct tactgcaggt gcgggagaaa
    51661 tcgttgtatt ctcacccagg gatttttgcc ctaccgagcc ttcgggttcg tgcaattgga
    51721 caaatgcaga cccgatatcc ggtgtatcca ttattgtctg gagtttgtag tccaggaaat
    51781 tgttattaag gggcctgcct gtttccgggt cgaacagcag ctgctcggac aatgcatatc
    51841 caaggctcat gctgacacct ccgtggacct ggccttccgc aagcatcggg ttgactataa
    51901 ctcccgagtc atgcacatta tatatctcga ttacctcaat ccttcccgtc tccatatctg
    51961 cttcaacctc ggcaaaagta actccgtatg ccattgcatt ggttctcaca ttgacagaag
    52021 catttccgca aataggttct gcatttaatc tgtcataata ggaccccatg gctatgtctt
    52081 caagtgtgca gatttcattg ccagactttc tttcaactat ccttgccgcc ttaatatcaa
    52141 gctcatcgat gcttaatccg gttttttccg atgccatctc cagaaccttg gatttaactt
    52201 cctccgccgc tttttttaca gccgcccccg atacaaaggt ttggcgtgag gcgtaagccc
    52261 caagatcaaa ggggctgata tccgtatcct gctgagatac tatatgcacc atatcatagg
    52321 gcaaacccag tacttccgcc gctatctggg acaaaacggt atcgcttccc tggcctatct
    52381 ccgttgcgcc tacctgaagc tgaaccgagc cgtcctggtt catgacaatc cttgctcctg
    52441 agatttcaaa accctgggga taggttcctg aggcatagct gaagcaagcc atacccagcc
    52501 cccttctttt tgtgcccttc tgttccttgt atttttccag tttttcgtcc catttgatga
    52561 gctgttttcc cttttcaatg cattcgggaa gtccgaaggt tctcacaacg atttcagaat
    52621 gaggatctgt ctttccggca cttatcaggt tccgcttcct gaattcgatg ggatccattt
    52681 tcatttcccg ggctatatct tctatatggc tctccagagc aaagaatatc tgcggacaac
    52741 cgtatcctct cattgctccg gctgccggaa gatttgtata aactgttttc ggctcatact
    52801 tcattgaatt aaagtcataa aggagcctgg acttaccgcc tgcactcatt gcaatagtat
    52861 ggccgtggga agcataagcc ccgttgttgc ttatgggcct catatggagg cttaacagct
    52921 tcccttcttt tgtcactgct gtcttcatgt agaatttcat tgcatggcgg gttctcgtat
    52981 caacaaagca ttcctccctc gtgagtgcat agcgtacagg ccttccattg actgcaaggg
    53041 acatggcggc tgcaataggt tctactataa tatcctgctt gttcccaaag cctcctccta
    53101 tgtatggttt gataattctg acctttcccc agggcatgcc aagagcttga gcgacaatcc
    53161 tcctggctat atgcggaatc tgtgttgaag aaacaatgac tatccttccg tcagcatccg
    53221 cataggcata tgcgctttga atctcaagat ggcagtgctg tacaatactt gtttcataaa
    53281 ccccttcaaa tatatggtca gcttttccga aatcctcttc aatatctcct atctcttcgc
    53341 ttgcatcccc caaaatatta tccggctttt cctcatgaat caccggtgcg ccgggcagca
    53401 ttgccttctc agggtcaagt acaaagggaa gtacctcata ctccacctca atcagcttaa
    53461 gagccttttc cgctgtaaat gcatcggaag ctacaacagc cgcaataccg tcacctacat
    53521 acctggcttt gtctgtaagt atcaacctgt ccgccgcatc cctgtgatcc ggttccaagg
    53581 accagggatg gcctgccgtt gcatatttca gcttaggcag atcttcatag gtaaatacag
    53641 cctcaacccc gggcaaagcc tttgccctgc ttgtattgat acgttttaca attgcatggg
    53701 cataagggct tctcaatacc ttccccacaa gcatatcccc ttcgaaaaag tcatcggcat
    53761 atttcgccct gcctgtcact ttggcaaacg catcttttct aatcgttctt ttccccacta
    53821 tttcgaaagc catttttcca ccttcaatca tacagatttt tcatgattta aaagcgcttc
    53881 catacttccc ttgccaattc acggcttctt gccatcattc tctcttcatc aatatttacc
    53941 agttttctgt cttccatcag aattctgccg ttcacaatcg ttgtatcaac aaaacgcccg
    54001 tttattccaa acaacagatg gccgtttata ttgccttcgt ttataggtgt aggcggatta
    54061 taatcaacta ttatcacatc agccattgct ccggctttca ggattcccac ctgtccatca
    54121 ataaacctct caactatttt cctgttgttt gcaaacagca ttgcgggaat ctcaccccag
    54181 gcaacatttg aatccccttt tacatgctta tgaattatat ttcctacctt gtaggattcc
    54241 atcatatccg ctgtatatcc gtcagtcccc aaaccaagaa gaattccctt gccgaacatc
    54301 tccggcaccg gtgaagcacc taccgcattc cccatattgg attcgggatt atggaccact
    54361 attgtgtcct tttccttcag catatccatt tctttttccg atatgtggat gcagtgcaca
    54421 gctatggtct tactccccag tacccctgaa tcgtaccacc tttcgataac ccctttccca
    54481 tatcttgccc tcgaatcctc caaatcctca attccctcgg cacagtgtac gtgaaaaccc
    54541 gtatcaaggc cgcttatggc atccaggcat ttgtccagtg ttttttccga aatggtcatg
    54601 gatgcatgaa ggccgaacat tcctttaagc atatcatcct ttttattgct gcagtacttt
    54661 ataaattcca cattttccgc aataccttct tcggcaatct tctcaccgtc acggtctgag
    54721 gtttcgtagc acaggttgct tcggatgcct atgtgttctg ccgcctctgc tattttgaac
    54781 aggcttcctt ttacggcata agggcttgca tgatggtcaa taactgtggt cactccgcat
    54841 tttacctgtt caatcatcgg gcccacggcg ctgtaataca catcctcaag ggtcagtacc
    54901 ttgtcaagcc tccaccacaa gcccttcaat atctcggaga actttgtggc cggcgggctg
    54961 tcgttggaca tacccctggc gaaggtactg taataatgca tgtgggtgtt aataaaaccc
    55021 ggcattatta gcttgccttt ggcatcaatg aaccttgccc cgctgtattt ctccttaagc
    55081 tccgaggtca gccccaattc cgctatctta ttgccgtcta tggcaataca tccgttttcg
    55141 atgattggaa tgtcatcatc tcttgtgatt actctgccgt ttcctactaa tatcattttt
    55201 ccccctcctt attcagtggt ttcttatgct gtttctttgc ctcttcacta tttcaacagc
    55261 tgttttatgc gcatctgatg ccacccggtc agaatcaatt gtcgtcagct ttccgtcctt
    55321 aactacgact ctgccgttga caacagtcat gtcaaccata ctggaattcc caaggcatat
    55381 gagagatacc agcgggtcat ggcagccgga gtattcgatg cctttcaggt tgaacagcac
    55441 tatatccgcc gctttaccta tatccaggca tcctgtgtca tcccgtccca acacctctgc
    55501 accgcccctt gtagccgttc tgagtacttg gtacgctgta agcccgtcag tgccgtattt
    55561 caggtggtta agaaggtaag cccttcttac ttcctcccac atatttgaac cgtcattgct
    55621 tgcgcttccg tctacagcta tggaaagctt ccctcctgca cggattattt ccgttgtcct
    55681 gcatatgccg cttgagagtt tcatatttga ggaggggcag tgggctatgc ctgagccctg
    55741 aagccttttt atttctccat cattcaaata aatgccatgg gcgaaccata catcatcccc
    55801 cacccattcc aggtcctcca tcagctcaaa aggcctcctg ccgtaacgct ccaggcagaa
    55861 tttctcctcg tcaagtgtct ctgccagatg tgtatgaagc atgaccccct tctcccttgc
    55921 cagttccttc gtctgcacca taagctccct ggtcaccgaa aagggggaac aaggtgccaa
    55981 ggctatcctc tgcattgaat aagggctgct gtcatgatat ttgtttataa gcctttcgct
    56041 gtcggcaagt attgcttcct cagcttgtac aacctccatg ggaggcagcc cgcccatatc
    56101 cctgcccagg gccatggagc cccttgtggc atggaacctt atgccgattt cccttgccgc
    56161 ccttatctgg tcatcaatga gtgttcccgg ctggttcttc gggaatacat agtgatgatc
    56221 ggaggtcaga gtgcagccgg tgcgcaataa ctctgcaaat cccaccagcg ctccgtaata
    56281 aacagcttcc ggggttatgt tcttccagaa ctcatataat cccaccagcc acgggaatag
    56341 gggcttctcc tgtacttcct ttatacctct gaacaaggtc tgatagagat gatggtgggt
    56401 attgacaaag cccggcagtg cggttaatcc gctgcagtct atcacttcag catctcccgc
    56461 agtgtcaagt ccgctgccta tctccctgat tgcagtcccg tcaatcagta tgtcgcagtg
    56521 ttcaaaggtc tcctccctgt cgttgaaggt gactatcagg tcaatgtttt tcaaaagctt
    56581 ctgactcata tttcaagtcc cccaaataac ttatttgtac cggtatttaa gaataatgcg
    56641 gcgtgtctga agttcgcatt cagccttcag agcactagcc ttgtatatga aggatatgta
    56701 aatgccgacg tggggaagct atattcgacg aaactttcca ccgacctcgg aacgggaagg
    56761 cttccaagca aaataaccag acggactcgc agagatggga cccgtgccag tgattttgcc
    56821 ggctttggaa agtgaggaga acatacttcc cctacagtcg gagtactttg caaacccttg
    56881 attttaagtc gcattcttct tatgtgctat ctacagcata aatgaatact taccgatgca
    56941 ggcctttatg attgcagcca tctctttgga cacaccattt tcctgcccta atgcatagtt
    57001 tactatttga ccttcctctg tccgtacaag gcatttgcac gctgccatat cggttaccag
    57061 gaagccctta ttgttgctgt ccctgaaatc ttcctcattc cagaaaaccg taaccttatc
    57121 cttgtacggg tttccttcat gggggcagaa tattccgcag ttgccgcact cgttgcacat
    57181 accgtccaga tgtataacct gatgcccgga ggcaaagcct tcgtcaacaa atatcactgc
    57241 atttgccctg tttggacaca catcaacaca caactcgcat atattttcgc aggagaggca
    57301 tctttcagct tccttatcac tatatacggg attattcaat atccctttcc tttcataaag
    57361 aatcttctca tccacgggta ataccttctt ttcaaaatca gccttcaagc cttccttgct
    57421 caatatatcc tttgcagccg cctttccgtc ggctattgct ctgacaatgg ttgcgggccc
    57481 ggctttagca tctccggcca catatacccc gggtatattt gtctcacagg cagagctgac
    57541 ctttggaaaa ccttttgaat ccagttctat tccgtttttt gcaagaaata tgctgtcaac
    57601 ctgttcacct acggcaacaa ttaccgtatc ggcatcaagg gtaattttct ctcctgttgg
    57661 cacggggctt ctcctgccgg aagaatccct gtcccccaga gccattactt cacatacaag
    57721 ctttccttct ttatatgctg ccggcgaaag cagttccctg aataatactc cttcggaagc
    57781 agcaagatgt atttcctcac ggtctgccgg catgagcgcc tttgtcctcc tgtatactat
    57841 ggataccgat tccactccgg gaaccctctt tgctgccctt gccgcatcca tagccacatt
    57901 tcctccgcct atgacacata catggcttcc taatttcata tttccctttt gtgctttgta
    57961 attctccagg aaggatattg catttaccgt cttttccgct ccctcctcaa gggatatctg
    58021 tccgggctta caagcgccta ttgccagcac aacgtaatca tattccttct tcaaagcttc
    58081 gacgctgaag ttttcattaa tgccaagctt gaatcccact ccgctccttt tgacaagctc
    58141 aagatccttt ctgaccatat cagaggatat cctgaactca ggaatcacat attcaactac
    58201 accgtaaggc ctttccctct tatccattac aacagcttcc attccgtttc tccttaggaa
    58261 cagagcagtt gcaagtcccg cagcccctgc acccactatt gccgcttttt tattgcttct
    58321 gatttctgcc ggcctgatac cggcggtata cttatcctgc gccttgaggg ctgctgtttt
    58381 cttcatttcc ctgataagaa ccgattcgtc ataatccaag cgggtacact tgtactggca
    58441 ttgatggtcg cagattgagc ctgtgattgc cggagaagca ttatcgctta ctattacttc
    58501 aaaggcttca tcatatttgc cttctcccac cagagcaaca tactgaggta tctgctggtt
    58561 aatggggcag cccattgcac agggagatac ggcacagtca tataaaggaa gctctgaggt
    58621 cagtttcctg ctgttaacgg gtctcaactc tttaaggtga tgcttatctg ttaacgcgtt
    58681 ctcagcaagg ctgcttattt ttgcgatgtc aatacccgca gccattctct tctgcagccc
    58741 ttccgccttg tggcccaact ggtttatcct ttcatagcct cccggcttca gtatcgtggt
    58801 tgcaaaggtt ataggcctta ctccggcttc cattaccttg tcgatattaa agaaatcaat
    58861 tccgccggaa taggagatat gcaaatctcc gttgaattcc tttgccagct tgcttgcaag
    58921 agaaattgaa agggcataca gtgatcttcc cgacatatac atttcttctc ccggcagctc
    58981 ctggttgaga atcttaaccg gcagtgtatt tgtcaatttt acccctatct ccagatttag
    59041 ttctcttgca agggctttta ggcgcttcag cattgccacg gcatccggga actgcaaatc
    59101 atgaatgaag tggtgcccgt tcagggttat atacccatat cccattgcat ccagggtatc
    59161 cctgacaaac ttttcgccga gaagtgtcgg gttcatcttt acaaaagtat gcagcttctt
    59221 ttccttcaga aaataatttg ctattctttc gatttcctgg ggcgggcatc catgcagcgt
    59281 agaaagtgta atggaagggc agaccacagg agaaatcata tccaggtctt cccttgtgaa
    59341 atgagtaaat tgattcatgg cggaaagcag tactttcctg cattccttcc atatatccgt
    59401 attggctgca ttgcgcatcc cttctatata tgcgtccatt ttaggggact taatgccctc
    59461 caggtcatag cctacactca tgttgaacat gaagtccctt ttttcgctta atccaagctc
    59521 cttcataaga acatgcagca agaaccatgc cttgatatac tcattatatg catcaggaac
    59581 ccttaattca gtagaccatt ctacgttgta gcattcatcc tgggcatata tgcatggttt
    59641 ggatacgggc aaatcttctc cgtcaagctt ttgtaccgtt ttcaactcaa tgaaacggca
    59701 gcctgtcaaa tacgcagcca caatattctg cgccagctgc gaattgggtc ctgctgcagg
    59761 gcccaaaggg gtcgccagct tttctccaaa caactcggtg aagcttccgc ttttattttt
    59821 gtaaaatttg cttttgtgga taccgaaaat tgagtccttt tcctttaatt cggacaacac
    59881 ccaggaaagc atcttctcaa aaggtatcag cctcattcta tcactcattc ttctcctcct
    59941 attcaagtac catatatgat ctcaatggca cagtatgtta atcagccaga ttctcagtgg
    60001 ttttcggcat tttatcagca tactttttta catagagcga gggaattgcc gcatacatgg
    60061 cagcggcttt caccaattcg tccttccagg tcttttcatt tggggcatgg gcctgatctt
    60121 catgacccgg tccaaagcct atgcaaggta tgccgtattt acccattatt gaaactccgt
    60181 ttgtggagaa tgtccacttg tccaataccg gctcatcctc gaaaagtcct ttgtatgctt
    60241 ccagcaaggt actgcatacg ggatggtcct cttctatgaa ccaggtgggg aaatacgctt
    60301 ctgtgggata ttccagcccc gtataggagg gcctgctgta tggatacatt gtcacctcag
    60361 ctttagctgc tttgaccgaa ggaagctccc tgatctgatt aagagcgtac tctccgcttt
    60421 ctcccgctgt cagccttctg tctaccgaaa tccagcagct gtccgccact gcacatcttg
    60481 acggagatgt atggaatatt tcagatactg tcagggttcc tttgcccagg aacggatggc
    60541 tgatgagcct ttcattcagc tctttcaatt ccatgagtat aggagccatc ttatatatgg
    60601 cgttgtcgcc cctttccgga gcagagccgt ggcagcttac accctgggtt gccaccttga
    60661 tttccattct gcccctgtgc cccctgtata tacggcagga ggtgggttca gtcaggacca
    60721 cgaattccgg gcggatattc atttccttaa tgatatactg ccagcataag ccgtcacaat
    60781 cctcttcctg tacagtaccc gtaatcagca atgtgaaatc atcctccagc ttcaggtcct
    60841 tgattatctt accggcgtat accattgcgg ccataccgcc ttcctggtcg ctggcacctc
    60901 tgccaaagat tatctcgtca ttctcatagc ccttgtacgg atcgaactcc caatttttga
    60961 tatttccgac ccctactgta tctatatggg catccatggc tatcaggtgt ttgccgtgcc
    61021 ctacatagcc cattatgttg cccatgggat ctaactctac cttgtcaaaa ccgacagccc
    61081 tcatttcctc ggcaatccta ccgataacct ctttttcacc gcagctttcg cttggaattg
    61141 ctatcatatc cctcagaaat ttggacatct gcggtttgta tttctctgca agctcaagta
    61201 tttgtttaaa atcatacgac atttcatgcc ctcctaatat cctaaaccgg ttcttttgtt
    61261 gaattcctct atcaagctgt caagctcagg cgccattttg tgcagccttc cccagtagtt
    61321 gtcatagtcg taccactgtt cattcagctc atccaggtca tacatctgct gctcgatcca
    61381 ggtgtagtat ttcaggttat gaatacgttt cctgctctgg taggtcagct cttccatggt
    61441 atctgtaccc agtgccaata catacctgtt atggtctata gctgcatcaa cattggagta
    61501 ctttcttccc cttgcttctt ccatctccat aagccttgac tggtacatct ctgctgaatc
    61561 tgtgagtact gtcattacta tatcattttc tgcaagctca tagtatttag caaacttgat
    61621 acatgatatg atgtttgcag cccctgaaat tcctacccag gacagctttt ctacaacctc
    61681 atcccttatg cccagggatt tcagatattc ctgtcctgcc ggttcattga agaggcggaa
    61741 caaacccaaa ctgtcattat catctattgc tataaccata tcggtattct ttacattatg
    61801 aatccacggc acgtgcttgt cacctattcc ctctatcctg tggtctccaa agccgttact
    61861 gagtagtgtg ggacactgca gtgcttctcc taccgcaagt tttgcatttg ggtaccgatc
    61921 ctttatgaaa tctcccgcac ccagcgttcc tgccgagcct gaagtcaggc atacccccgc
    61981 cacttttcct tttgaacctg cctcagcatt gattatgtct tccattgcat ggcctgttac
    62041 ctcatagtgc cataagtggt tgcccaactc gccgaactgg ttgagtataa caacattgtc
    62101 cctggtcctt ctcaagtccc aggtcttgtc atatatttcc tttacattgc tttcgcagcc
    62161 cggtgtggca ataacctcac cggcaactgt cttgagccat tcaaaacgtt ctttgctcat
    62221 gttctcaggc agtattgcta tggagctgca gccaagcagc gtcgagttat atgctccccc
    62281 cctgcagtag ttgcctgtgg aaggccagac tgcctgatgg taggtagggt caaactgtcc
    62341 ggtgaccagt cggggaacca ggcatgcgaa gcttgcacct accttgtgtg cccctgtagg
    62401 aaagaacttg cccgccatag caaatattct ggctttaacc cctgaaatct cccttggaat
    62461 ttctatataa ttaggcaatt tattatacag tccgccgtgc ttgacgggct cgttcttcca
    62521 ggatattctg aaaaggttga taggatccac atcccacaag cctgtgttct tcaatttttc
    62581 ctttaccttt gcgggtatct tgtcgggatc cttcatctgg gcaaaagtcg gaataactat
    62641 gtttctttcc tttacgttct gcaccgcctt ttcaagccct ttttcgttga ttgtaaaatc
    62701 aatgagcttt ctcatttttt cccctcctaa gcttatttac aatttaaatt tactattacg
    62761 ggaacgcctt aaatgtgcac aattcgtttc agatcattca ggtcaggttc cacatggttt
    62821 gcttttcccg cagctttcac tgcactcttt atgtccttca gcccgcttcc gctgactatg
    62881 cttactacct tttcatcacc ttttaaaagc ccaagctctt tcatcttcat aattcctgca
    62941 aagcttgtta ccccggcagg ttctccgaaa acccccgttt tttgcgccag tattttcatt
    63001 gctgcaagta tttcctcatc ggatacatcc accatgtacc cccctgattc gtgcagggcg
    63061 ttcaaggctt tgatgccgtt ccggggtatc cctaccgaaa tactgtccgc caaggtatca
    63121 ggcactctgt attcaaattc ataagttttc ttattaaaag cccttgtaac cggatttgag
    63181 ttttccgcct gaacccctac tattttgggg aacttgtcaa tgagtccaag ctcaaagcac
    63241 tccttgaagc ccttccatac ccctcctatg gtgcagccgt cccccacggc caccacagct
    63301 atatcgggag ccttccagtc cagctgttcg cagatttcgt atgagattgt cttcttgcct
    63361 tccaccaggt atgggttgat tgcacagctt ctgttgtacc agccgaattc ttcggcagcc
    63421 ttgaagcaaa gctcaaccgc ttcatcatag gtgcctttta caaggaatac attggagccg
    63481 tatatcaaaa gctgtgtcac cttagcctcc ggagctgttt ccggcacaaa tatataggtc
    63541 ttaatcccgg caaccgctgc aaatcctgac aaggaagaag ccgcatttcc cgttgaagcc
    63601 gcgcacatga cattccttcc aagctccaca gcctttgcca ctcccactgc acttgccctg
    63661 tcctttaacg aagctgtggg atttctggag tcatccttga gaaaaaaact gctcagtcct
    63721 gtttcctttg aaagggactt tatattgtac agaggtgtcc atcccacttg aaggggctgt
    63781 attccgccgg ttttctccaa aggtattgcg ggaagatagc gccacaggga ataatccttg
    63841 ttgtttttca gatattcccg ggtcaattcc ttcctgatac tgtcataatc cagttcaacg
    63901 tccaggattc catctatgcc gcaatcacgg cacgtataaa gtatctcagc ttcattgtat
    63961 tctcttccgc acttgacgca tttcagcttt aataccttgc tcattcctcc accttctttc
    64021 tatcataagg ggttccctcc agggggagtt caaaccttct gatgccgtca aacctttgat
    64081 aggctgaagc tacggcagca gccaccggaa tcagtgatat ctctccaatc ccttttgcac
    64141 cgtaggccag ctctgattga ttcttttcta tgatttttac atctatttca ggtatttcgt
    64201 ccgccctcag caagccaagg gttccgaacc tggattttaa aattcctcct tcatatatca
    64261 gcctttccct caatgccgag ccaaggccca ttactacgcc cccctcgatc tgtccttcga
    64321 tatttaaagg gtttatcgcc ctgccaatgt catgggccgc cactattttt gcaatcctgc
    64381 cttcggcatc gagtattgct aaatgtgtgg catagctgta tgccacatgg cttacaggat
    64441 tttccctgct tgaatttatc ggatcagtaa ccccactata ttcaccttca aaaactacac
    64501 cttccaactc ttccggagtt ttttccgcca atgccgcctt caacttgagt gcagcccgtc
    64561 ggcaggcttc accggtaaaa agagtctgcc ttgaggctgt tgttgctccc gagtcgggac
    64621 acaataagga atccggctga ctgaaaatta tgtcagatcc gtcaataccg agggtttcag
    64681 acactatctg aatcattacg gtagccagtc cctgccctat gcaggaggcg gcagaatata
    64741 caacaacctt gccctctttt attcttatac ggcagcgccc gacatcaggc aagcctactc
    64801 ctatccctgt attcttaaaa gcgcatgcta ttcctgcata tctgctccgg tagtattcat
    64861 ccctgactgc aaggagtgtt tccttgacag ctgtatcatc gccgcatacc tggccgtttg
    64921 ccagcaccat ccccggttct acggcatttc tgtagcgaaa ctcccacctg tcgatgcctg
    64981 ccaggtctgc cagctgatcc atgttggcct ctcccgcaaa attggactga ggcactccaa
    65041 agcctctgaa tgcgcctgcc ggggcattgt tggtatatac acagaagccc tccagttcca
    65101 cattggggat actgtatgga ccgcagacat gagtacatgc ccgctccaga accgcagtcc
    65161 ccaaagacgc ataagcgcct gtgtcggcta ttatccttgc agtcatgaag gtcagcattc
    65221 cccgttcatc acagcctgtt gcaacctcca gttccatacc gtgccgtttt ggatggcata
    65281 acaggctttc ttccctggta aaagtgtatt tgcatggctt ctttgtataa aaggcggcca
    65341 aagctgcata atgctgggcg ctcaagtcct ccttgcctcc gaagccgccg cctacatttt
    65401 gcagaatcac tcttaccttg tcatgaggca atcccagaac acggcataac tcatgatggt
    65461 cgtgggtgat gctttgggta gcggcataca ctgtcagaac atcgacctca taaaaggctg
    65521 ccgcgctttc aggctcaata aaggcatggt caacgggagg cgtcctgtag gtgttcttca
    65581 ccacatactt ggactctgcc attgcttttt cgggatttcc cctttttaca taagtcctgc
    65641 ttaaaatatt gcccttctca tgaattttcg gcgcatcgct cctcatggca tattttgcgt
    65701 cggtaacagg ctccatttct tcataggtca cattaataag ccttattgca gcttccgcca
    65761 gttctttagt ttcagcggca accgctgcta cggcatctcc tgcatagcgg gtcatttctc
    65821 ccacaggtac aaaggtgggc caatccttga aaatataccc ctgataattt tctcccggta
    65881 tgtcctctgc tgtcaggaca gccttcaccc cttccagggc tttggccgcg tctgtatcga
    65941 cggacagcac ccttgccctg ggcttcattg ccctcagaac agaaacataa agcatatcct
    66001 ccatgaaaac atcatcacag taagttgctt cgcccagtgc cttctgtatg gcatctaccc
    66061 tgcgccaatt ctgtccgata ttggaactgc atgtactcaa gctgatatgc tttgattccc
    66121 ttaaagcttc ggcagcaagc cttattgcct tgacaatctt cacatagcct gtgcatctgc
    66181 agatattatt cctgattgcc ttctttattt cttcttcaga aggatccggg tttttatcaa
    66241 gcagcgcctt tgaagccaac atcattcccg gggtacagaa gccgcattgt acggcccccg
    66301 cttcagcaaa agcatttgaa tacacttctt tttcttttcc gtcaagccct tcgacagtaa
    66361 gcacttccct gccctgcagc ttatcggcct tcagtgtaca tgaccttgct gccttaccgt
    66421 ctatcaaaac cgtacagctg ccgcataccc cgctgtcaca gccattttta acagatgtca
    66481 gcctttcttc ctcccgaagg tatttcaaca gattggttcc ttcccgtaca tttactgccc
    66541 ttccgttcac cttgaagctg atcaagtgca tcacctgcct tatttgctct atttaacgaa
    66601 attaagtatc atattttctg ctgcatcaaa ataagcgtcc agattgaatt taccttcatc
    66661 aataagatac tgcatagatg ctcccatcat tatggaaact aacaaatacg gaagcattgc
    66721 acattgctca gatttacatt ccggaaaacc cggcatctga ttcaatacct ctgttatgtt
    66781 acttctccat atattaaaag cgcctatgaa tttttccctt atttcagggt ttgcggttcc
    66841 ctgtacccag aaatcaaact gcacataatc aatttttttg tggttatata tgtcatcctt
    66901 cttctcatga aaaagccccc ggagattttc ttcaaaagac tttttctcca gatccactga
    66961 actcatcctt ttctcggtga atctctcctg gattctgtcc agcaatgcaa tcatcaggtc
    67021 atttttggta gggtaataat aatgcacgtt ggattgtacc acccctgcct cctttgcaat
    67081 caaatgcatc ctcgtgccgc ttattttatt ctgcgcaact atttcaagag ctgcgtccag
    67141 tattttgttt gtaacttcat tagacataac agtacctcga ttttcgttaa aaatatacct
    67201 tatctatttt ttgccggttt atttcccgga gaacctgggg taatttgaat ccatcagcat
    67261 ttcaaaggtc tcaggcacat ttttgacctt gctcaggaat atcatcgcag caataatgta
    67321 cggcttataa ccggcctgtt tatataaagg tgtacgatac cggtcaaaca cagatgcggc
    67381 aacttcccct tccttgcagc tgactccggt aatatcagca ggcaggcagt gcaaatacag
    67441 ggctttaccg ttctttgtaa gcttcatcat ttcttctgtg cattcccagt ccttatgccc
    67501 ggcgttctgg gcaaggagtt ccttttcaag agctttgatt tcatcaaact ttccttctcc
    67561 atagagattc gtcctcttct ccattgcggc atagggagcc cagcttttgg gatataccac
    67621 atcggcattt gcaaaggctt ccttcattga attggtcttg gaaaatttcc ctcctgattt
    67681 tgcggcgttt gctcttgcta tatcctcaac gtctgacagc agttcatagc cttccgggtg
    67741 agccaaagtc acatccattc ccattcttgt catcaggcct attaccccct ggggcactga
    67801 caaaggcttg ccgtaggaag gagaatatgc ccatgtcatg gctatctttt tgcccttaag
    67861 gttttccaca ccgccgaaat agtttattaa gtgaagcata tcggccatac actgtgtagg
    67921 atgatctatg tcgcactgca gattcaccag ggtcggcctc tgctccagga ctccctcttc
    67981 atatcctgtt tgtacggctt tggaaacctc cctcatatat gtattgccct tgccgatata
    68041 catatcgtcg cggataccga taacatcagc cataaaggaa atcatgtttg cagtctcccg
    68101 cactgtttcc ccatgagcta tctgggactt cccttcatcc aaatcctgta cggtaaggcc
    68161 cagcagattg catgcgctgc tgaagctgaa cctggttctt gttgaattgt ccctgaacag
    68221 agatactgca agcccgctgt cgaatatcct gggagatata ttgttctccc tcattttgcg
    68281 gagtatttcc gccgcctgga atactgcatg aatctcatca tcggtttttt cccatgttaa
    68341 aagaaaatca ttgttgcaca tagcattgct gtcaagcttt ttgaattggc taaccagctt
    68401 tcttatttct tccatctgca tctcccctac tatctaatat ctttataatt tccatatgaa
    68461 catattgaga aaaatttgat tgtctagtca atataattat agaatgttta gtaaattctg
    68521 tcaataaaaa tgctaagcat ttttcgggta cgaggttcga ggttcgaggg aggacacggg
    68581 gacgtttcga ggacacgggg acgtttctcc tgtcccgcta aaagcgggac aggagaaacg
    68641 tccccgtgtc ctcgaaacgt ccccgtgtcc tcttctataa tccttacttc ctaaaacact
    68701 tcacaaattt gccccatccg ggctctccca aaaaggagcc ctcctcatac accagtctgc
    68761 cccgggagaa ggtcataacg ggatagccgc tcatttcaca gtctttccat atggtgtagt
    68821 ctacatcgga atgcatgttt tctctagtca caataaattt cttttccgga tcatatataa
    68881 ctatatcagc atcgctgccc acagctaaag ttcccttttg cggagcgcag ccgaaaatcc
    68941 ttgctgcatt ggcggaacaa acctctactg ccttattgaa ggtaatgttc cctttatttg
    69001 cctcacttaa catgtagggg tacatgttct caactcccat gcagccattg ggaatctttg
    69061 tgaaatcgtc cttgccccaa tccttttcat aagactggaa agggcagtgg tctgtggcaa
    69121 ccgttgaaat atatcctctt ctcaggcctt cccacaatgc cctccggctg tcctccccct
    69181 taatggaagg agagcatacg aaattcctgc catcctctct cttatacact tcattgttga
    69241 aattcaggta ctgggggcag gtctccgcat atatttcaaa gccttcgtcc ctggctcctg
    69301 aaacttcctc caagccttcc ctgcttgcca gatgcacgat gtacagggga gcatcgaagg
    69361 ttctggcaag atagaccatt ctccttaccg catctgcttc tacgaactcg ggccggcttt
    69421 cataatgata ccatgccgat gttttcccct gtgacagcag cttctttatt cttctgtcaa
    69481 taatgtatgg gttttccgca tgtacggcaa ccaaggcacc cgtctccttt gaccttacca
    69541 aagcatcggc tattaccccg tcatccacca tcatatcttt gtaaaccata tacagcttga
    69601 agcttggcac tccgaactct acagaagcct tgaactcatc aaggacttta tcggtcaggt
    69661 cggtaacggc aatatggaag gcataatcaa cacaagcccg ggacgcaaca tatcccagcc
    69721 tgctgtctgc tgtttccaca agacccatgc ctttctcctg ggtaacgaag tcgaatacca
    69781 tcgtcgtacc cccacaggca gcagcccgtg taccggattc gtagctgtcc gccgagacgg
    69841 tttctccgac aggcagctcc agatgcacgt gagcatcaat ggcacccggc agtaccaatt
    69901 tgccggcggc atcaacaacc ctttcacctg ctgcctgtat attccccgaa atcatcgcaa
    69961 tcctgccgtt tttcacagca atatcagctt tatatgactc cgaagcagta atgattgtcc
    70021 cattcttgat tatcaagtcc atatcttcac tcataaaagg cacttccttt tatccgttta
    70081 ttttgcaagc agctatatat caataaaact gaagcttcca acatcaaaga ggcccctatc
    70141 cgttaccctt aactcgggga tgaccggcag cgcaagaaat gacagggtta cgaagggatc
    70201 aattccgtca ggtattttaa ggctttttgc tgactctatc atgccggaca gcttgttttc
    70261 cacaaaaaat gaatctctgt cactcataag gccggctatg ggcagcggca gcgtacctgt
    70321 tacctctccg ccgcttacaa cggtataacc tccgttagct tccttcagta ctttcgcagc
    70381 caggagcatg tcagcatcgt tatcgccgat tatgataaga ttatgggaat catgggcaac
    70441 agtggatgct atggcaccat tcctgatacc aaagcctttt acaattccaa gccctatgtt
    70501 tcccgtggct ttgtgccgct ccacaacagc aatttttgaa aacacctcat ccggtatgaa
    70561 attgtttccg ttgtccttgg gcactgcaag cctttctttt tttgtaatca gctgattcgg
    70621 gatgatggtt ataacatttg ccttatcacc tttaattttt attttcaggt gttcagggga
    70681 taagttaggg attcttactg tgtttaatac atttgggtca tctatgattc tgcttatatc
    70741 aggctttttc cccttttcac aaacaaccga tccgcggaaa agcacctttt caatatcaaa
    70801 gctgtttaaa tcggacagaa taagcaaatc agccgaatat ccgggggcta tagctcctaa
    70861 gcgctttaaa tggtagcact tggccggatt tattgttgcc atttttattg cttcaatagg
    70921 gtctacaccc aggctgattg ccttttttac attgcagctt atatggcctt ccctggaaat
    70981 atcctccagg tgcttgtcgt cagtgcaaaa caggcagtgg ttcagaggaa cgtcccgttc
    71041 aataagccct gatataatgt cctcaaggtt tctggcggct gagccctctc ttatctgcac
    71101 atacatgcca agcctcagat gtgcgatgac ctcatcaata tttgtgcatt catggttggt
    71161 cagcacacca acagccttgt atgcacaaag ctcatgctcc gataatcccg aggaatgtcc
    71221 gtctacaacc gcattatcat aaagctcgat tttgttcagg atatccctgt ccccggccag
    71281 tactcccggg taattcataa cttctccaag cccgaggata tggcagctgc ctgcaaaggg
    71341 ctccattacc cttgccgtaa gagcggctcc gttgttttca aaaggagtcg caggtacaca
    71401 ggaaggcatc atgtaaaata tattcatagg cacgttccgg gcctcattca gcatgaaatg
    71461 aattccttca acaccgcata cattggcaat ttcatgggga tctgcaataa tcgtagtcgt
    71521 tccatgaggt aaaacaactc ttgcaaattc agccggagtt atcatgctgg attcaatatg
    71581 aacatggcag tcaataagcc ccgggcaaac atacatgccg ccggcatcaa tctcttccct
    71641 gcctgaatag tctccgacac caactataag gctgtcatgg attgcaatat cgccggatat
    71701 tatttcattt gtaaacacat tcacaatact ggcatttttg attgccaggt ctgctttttc
    71761 cctgcccgcc gcaatctcta ttcttttttg tttctgttca acactcatct caaatccctc
    71821 atatgcttaa ggctgccgcc tttttttaca agcattatct cagctataat actcaatgct
    71881 atctcctgat gggagtcccc tccaaggccc aagccgatag gtgcatacac ggcttccagg
    71941 tcctcctttt tacagccttc actgagaagc ctgttaaaga caaattgtgt cttctgtcgg
    72001 ctgcctatca tcccaatata acctgcgcct tttcctattg ctgctcttag cgcatccgca
    72061 tcgcttgcat gccctctcgt tacaatgact acatagcagt cttcatctat gggatattta
    72121 ctcatgcact ctccaatatc tcccacatgg atttcacagc cgggaaaccg ttcattatta
    72181 ccatattcct cccggtcctc cattatgaca atatgaaatc ccatcagctt tccaagctta
    72241 tgcagttcaa gggctatgtg ccctcctccc gcaataacca ggctgtcttt tgccttgaat
    72301 acctttataa agatatttgc cttgcccccg cactgcatat ggagttctcc ggtatcatca
    72361 agctccatct ccattagctt gttctttcct tcactcatac agagaagagc atattcccgt
    72421 gctttgtttt caagattgcc gccccctata gtgccaagag tgcttccatc agcaaataca
    72481 gccatcataa agcccttttt gcccggtgat gaaccctgct cctcgaccag agtcacaagt
    72541 gcagcctttc tgtttgcctt tacttctgcg gcaagcttct ctaaaatcgc atactccata
    72601 atctcacctt ccatactttt cttttctgag tgttaatatt gcttccagca ctccccctgc
    72661 taccgacctt gacttgtccg atattgtaaa gcaatattcc cttatgcccc tggggtctat
    72721 atctcctatt ttcaaaccct tctgtacata agtgttatct gcaataagcc cccttattac
    72781 tccgtctata ttggatttta ccggctcatc atctacaaat gaaaccacat ctccctgctt
    72841 cacaatatct cctatctcac gggtattcct tgttataccc gttaccggtg cacgaagcag
    72901 ccgttcttgt gaataaccca ttatatatcc cggaattcct gtatcggcgc aagctgttcc
    72961 cttgtatatg acctttccca aatcatgccc tctattagtc tcaaccacgg catcaacgtc
    73021 cgcccccgcc tcgaatccgg gaccgacgcc aattgtgaca ggagccgacc ctctatatgt
    73081 tcccgtgttt ctttttgcca aaacagcatc aacaatcacc aatggtttta tctgttcaag
    73141 tatcttcatt tcactgtcgc acagtaccgg tatatggcta ttattccata tttcatatat
    73201 gtcttcgata cattccgctt ttaccgcacg gacaccttcc acttccgctt cacctttcag
    73261 aacggcattt gcaaaggaag aaaaacgcct tacgaccgtt ggtttctcaa tttcggtgag
    73321 aagcagcctg aagccgcatc tatgaagcct gtgccctatt ccggttgcaa tgtcccccgc
    73381 accttttacg ataataatat catccatttt cgccctccag ctttgttatt gcttcatatt
    73441 cttcccaggt atcaatatcc aggcccggtt tcgcatcagc aaaattgaca gtaatgattc
    73501 cctctttcat ttcttcaagc aatacgcgcc ctcccgaatc tcccgagagg ctcataagct
    73561 tatttctcag ctttgaagca aatatcacag ggtttccttt tattccatta tacaggggaa
    73621 ctacggcatt gcaatgcccc ggtaaaaatt ttccaatcag tttattgata atactttcat
    73681 cgagaaaggg ctgatccccc gcaataaaca taaaaccttc ggcagaccgg caggaattct
    73741 taacccccaa accgacagaa tggctttgcc ccgtttcagg agcattattc tccacaattt
    73801 ttgcccggta tttcttccct atatcggcca cagaatcgct gccgcacact aatataattt
    73861 ctccaagctc cgattttgag gccgctgcta taaccctctc aattacggga attcccccaa
    73921 ccggaagcag cagcttgtcc gtacccattc tcctggataa tcccgatgca agtataatgc
    73981 cgctgatcat attttcacat ccccaatttt gtaactacta tcccgtcagg ctcatacccc
    74041 atatcagaaa gcctttgaat aattttgccg gctgcagacc attccttttc cccgtctgcc
    74101 ttatttaaca ccacatatcg ttcagcccgg gaaggaacgg ctttgaacaa accctcttca
    74161 tgggcgatta atctgccgat aatatctgca tcgataatat ccccctcacg gcagcccgtt
    74221 atattgcaga agatttccgt cctgtggaca ttttccgggc ataccttttt tccgacacaa
    74281 tcaaggccaa ccagcccaag cactctggaa gcaagggagg gtatgacagg ctcatgctcc
    74341 gcaggcgcct tcagaggcct ccctcttgaa ccgtctcctt ctacgattat ataatcaaat
    74401 atgtctttta aaaaaagggt atccagaaat tccggatcaa cccccagcag cttgccttct
    74461 ctagaaagtg ccctgccgta aacagttata ccgcttctta tgctcttaaa caaatccggc
    74521 agcaaaagat ccgcgattac tgtttgaaca ttttgattct ctaaaggata ataaatagct
    74581 gtggtagttg tgacaagaac cttttttccc gtacccgaaa gttctttggc cagcctgaac
    74641 attgcggagg ttttaccccc tgctcccacc aggcaaatca tctctttctt ccatatgctg
    74701 atatttactt gactatataa gctttccata tcttacattc caaatgtaca gcaagggtat
    74761 ctgcagcgtc tttcacccta atgcttctct tggaacctct cctcctccgt tactatatag
    74821 tcatcaatat ttctatatat atgggaaaaa atccttttta tagcgcagga aaagtattgt
    74881 atttttataa atgaattgta tcaaagacga ataaatatcc cccggcatgg ccgggggttt
    74941 attgttatac acaaaaatac accttctgca ttaatcctga cagaaggtat acataatgca
    75001 aatatttacc ttagtacgtt cttttgttcg gtgcctggca cataagattt aaaactgtcc
    75061 cctgaaatca gctcagcaaa ctcagctgtt ttctgccgga ctagcatgct ctgcagattg
    75121 tcctgtgtcc gtccgagttt ttttgcactc tcccgctgct ggtgatatat atattccgta
    75181 agctcagctt ttaattcttc atctgccgtt cgggaataaa cctttaatgc gggatcaatc
    75241 cccgcaggac ccgactgata caatatttgt acatcaaagc tttttagtgt tcccgcaagg
    75301 taacggtcag cattatagga ggcgacaaaa ccgtccggat taagcaggca aagggcgcac
    75361 ataatcaaag tacctgtaaa agctgaaagc cgcacaattg agaactgcct tttctgcagt
    75421 acaataacgc cgacaaaaac aacggcaagg aataccatga ataaacaggg cagcaggcgg
    75481 cgtatggaaa gcccgtacgc tcctatataa agcgccatct tgctgaatgc tgcggcaatt
    75541 aacagcaaag tcagcagaga aagcaggcag ttaagtgttt ttaatatata gttcttttga
    75601 ggttttttac aaaataggtt tgccaatgtc agcaatgtca ggttaatcgc agcaatttgg
    75661 cacagctcaa aaaatccgct gcgggcgtat tcggagtata tctgccaccc ctccggcctc
    75721 tgtcctgcaa aggctgagaa aaagtaagga agctggctgc cgataaatac catatacaag
    75781 cagcatatta aaccgagcac ggtgtaaaca gtagcggctg caagtaccct catggattga
    75841 accccatatt ggataccttc tcttttaaac gtattgcagc ccctgttatg ggcacagcct
    75901 gctacaagcc caaataaata agctgctaca ggtatcgaaa ggataatgtt gaatattgtt
    75961 tctgaaaact gattctgcat ccatcggaaa tattcaataa gcccatttgc aattctggag
    76021 aaccctccgc tgtccgctct caaaagcagc ggcaaaacca tgcccgcaat caataacgtc
    76081 agcatcaatc ccagtgcgat ggataaaaac tgcttaccaa agcttctctt aatatgtatt
    76141 gatgaataaa ggcttttgta ttgacatccg aagtttttaa acggaataac aaacatggca
    76201 ttaatcccgt ccagtatgag ccaattgctg gtattggcaa gaatcaatcg tcctgaggca
    76261 ctgattacat agtaaacggc cccacagaac aggaacaggc tgcgccacgg ctccaagcca
    76321 ttattagaat ataatgaata gcttattcct actaatacaa ccacagccag ccaaaagaaa
    76381 cctgaacggg taatggttac tccttttttt ataaaataca ttgttacggc aatgtaatat
    76441 ccaaatgtaa acactgcaac accccatccc tgccaggaaa acattaccca tcttacaaag
    76501 aaaaacccca gcacaaatgc aagcaccgca aaaattgtat ccctgctgtc agctatgaaa
    76561 ggttctttgg ccccatatgc cgccggtatc cctcctccgg ctgcctgcag cacttcgtcg
    76621 gcagccagtt gtccgttatt acttaaaata ttccctttca tatataatct ccttattctt
    76681 agtcttactc cctgaattcc agaatatcac ccggctggca ttccagagct ttgcagattg
    76741 cctccagtgt tgagaaacgt attgctttgg ctttaccggt cttaagtatt gaaagattgg
    76801 cctggtttat atcaatcttc tcagccagtt ctccggagga catttttcgc tttgccatca
    76861 ctacatccaa attcacaatt atgggcatat ccccttcctc ctttaaatcg taagttccga
    76921 ttcgttctta agttcaaccg cctgcgcaat aacattcttg ataacccgga caatcagccc
    76981 cataaatgcg gctgctacag ccacaaagca ccacggaaaa tagtaaggta ttgaagctaa
    77041 gcagataaca gatcccgcaa aacaacacca ggaaatccgc cgaaggtatg ccacattttc
    77101 tgtgataaaa acctgttcaa gctcaatttg atgaagaagt cgatagaggc agtacagcag
    77161 tattgctgca ggaatactgc cggagtatat ggttgacagg aataaagctt ttttttcggc
    77221 taaatcaggc cgaaaccacc ttataagcca gggggccgat aaaacggttc ctatcagcat
    77281 aacagcaaat accagcacgc agaacttgct taaactaatt gatttcctac cgctccacat
    77341 aaaatcatcc tccaaaattt catgaaaagt cttatttcac ttccatcgtt tgtagtatag
    77401 catatgattt attgatatgc aatataattt tattgttatt caatatattt ttattgatta
    77461 ttttattggg gactgcattt cggt
//
LOCUS       DAOQUR010000006        76327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_386_length_76327_cov_10.274937, whole genome shotgun sequence.
ACCESSION   DAOQUR010000006 DAOQUR010000000
VERSION     DAOQUR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 76327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76327
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_386_length_76327_cov_10.274937"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1184)
                     /gene="tsaB"
                     /locus_tag="PKI50_02195"
     CDS             complement(48..1184)
                     /gene="tsaB"
                     /locus_tag="PKI50_02195"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNT02560.1"
                     /translation="MKILAVDTSTSAATAAIMEDDRLISEYILNISRVHSQKIMGIIE
                     ELFNKSDIKPAEIDLYACSAGPGSFTGLRIGASIIKAMAQTFDKPVVGVPTLDALACN
                     LYNCTGLVCPMLDAQRGMVYSSLYSYNSGRLTKLEDLGVIPIEDLIELVKSKAVPTTF
                     LGDGAVIFEENLKKGLEGYNAAPAGSLLPRASSCARMALEMYNKGSFDTYNSFRLTYI
                     RKSQAEAEYEKKNKAEFQSMSLDDIDQVVEIENLSFASPWSRESFESELLKNKLARYI
                     VAKVNGKVAAYGGMWIILDEAHITNIAVHPEFRERKIGEKLVKEMLRTAKENKAEHIT
                     LEVRASNDAARKLYEKLGFKDSGIRKGYYADTGEDAIIMWNELS"
     gene            complement(1165..1641)
                     /gene="tsaE"
                     /locus_tag="PKI50_02200"
     CDS             complement(1165..1641)
                     /gene="tsaE"
                     /locus_tag="PKI50_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNT02561.1"
                     /translation="MLKFHTESAEETAKIGEGLGRLLSPGDIVCLSGDLGAGKTAFTQ
                     GIARGLEVKDYVTSPTYTIINEYEGRVPLYHFDVYRLNDVEEMYELGYEEYFFGDGVV
                     VLEWADMVSDIIPGERLWITILNTKGDNSREIIMEPAGEAYEEIMKGMESNENTGC"
     gene            complement(1707..3833)
                     /locus_tag="PKI50_02205"
     CDS             complement(1707..3833)
                     /locus_tag="PKI50_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNT02562.1"
                     /translation="MDIIAKLAKELSVKPWQVENTVKLIDDGNTIPFIARYRKELTGE
                     LSDTTLRELGERLTYLRNLENRKEEVLRLIEGQGKLTEELKASIEKAEVLQEVEDIYR
                     PYKQKKRTRATIAREKGLEPLADIIFSQERQSKALEEIAEAFVNDELGVSTVQDAING
                     ALDIIAEIISDNAEFRKEIRELTFGNGTIVVKGTKEEKSEYQMYYDFSEPVKKIAPHR
                     ILAINRGEKEEFLNVKAEAPADRILTYLNNSTIKENSPYKNMLEETVKDAYNRLIAPS
                     IEREIRNNLTEEAEEQAIKVFAANLRNLLLQAPIKGKVVMGFDPAYRTGCKIAVVDDT
                     GKLLETAAVYPTPPQNQAEEAKMILKKLIKKHNVEVISLGNGTASRESERICAEIIKE
                     MNMPLHYVIVNEAGASVYSASELASQEYPDINVSLRGAISIARRLQDPLAELVKIDPK
                     SIGVGQYQHDVAQKKLSDSLNGVVEDCVNSVGVDLNTASPSLLQHISGINSAIARNIV
                     SYREENGKFRSRRELLKVKKLGEKAFEQAAGFLRIPESENILDNTSVHPESYDVAGKL
                     LEKAGRDISAAVDKLSAVDVNELAAELGVGVPTLRDIINELKKPGRDPRDEMPKPVLM
                     SDVLDIKDLKPGMILTGTVRNVIDFGAFVDIGVHQDGLVHISELADRFVRRPLDVVQV
                     GDIVKVKVLDVDLDRNRISLSMKGLN"
     gene            3979..4419
                     /locus_tag="PKI50_02210"
     CDS             3979..4419
                     /locus_tag="PKI50_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT02563.1"
                     /translation="MVNKKMFIVDSDILPKAYEKVIEAKLLLRTGDAATIQEACRMAG
                     VSRSAFYKYKDSVFDYTAQERGRTVTLFMILKHIPGVLSDIINKIASVKGNIITINQN
                     IPVMDSASLTASIDTKIMEINPEELVELLLSCKGCKKVEIIGME"
     gene            complement(4422..5529)
                     /gene="prfB"
                     /locus_tag="PKI50_02215"
     CDS             complement(join(4422..5453,5455..5529))
                     /gene="prfB"
                     /locus_tag="PKI50_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_106406468.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNT02564.1"
                     /translation="MLELEQYKYGLSEIWDAIEEIEVSLDLTSIQERIYELEQEMNQE
                     GFWNDAENAGKVLKTIKALKDKTEKIENLKKEYEDIEVLIELGMEAQDESVIPEVEKS
                     FKTLQKDVESFRIETLLKGQYDRNNAIFTIHSGAGGLEAQDWAEMLLRMYTRWAERKG
                     YAITILDMLRDYEAGIKSAALLIEGENAYGYLKSEKGVHRLVRISPFDTAGKRHTSFA
                     SVDVMPELDDEVKVEIDEEDLKIDTYRSSGAGGQHVNKTDSAVRITHIPTGIIVQCQN
                     ERSQHSNKEMAMKMLFAKLLEIKEQEHKDKIDDIKGEYREIAWGSQIRSYVFHPYNLV
                     KDHRTGEETGNTGAVMDGELDDFINAYLKQGNGV"
     gene            complement(5543..8041)
                     /gene="secA"
                     /locus_tag="PKI50_02220"
     CDS             complement(5543..8041)
                     /gene="secA"
                     /locus_tag="PKI50_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNT02565.1"
                     /translation="MFLDKVFGNPNDREIKKLMKTVQEINKLEPAMQKLSNEQLQSKT
                     DEFKKRLADGETLDDLLVEAYAVVREASVRVLGMRHFDVQMMGGIVLHQGRIAEMKTG
                     EGKTLVATLPLYLNALTGRGTHLVTVNDYLATYQSQWMGKLYNFLGMSVGLIVHGLEP
                     EDRQKAYRADITYGTNNEYGFDYLRDNMVIYRDQMVQRELNYAIVDEVDSILIDEART
                     PLIISGVGEKSTQLYYVADTFVRGLKNEADFTIDEKAHTVVLTEEGVAKAERFFNLEN
                     LADANNMEIQHHINQALKARNLMRRDIDYVIKDGQIIIVDEFTGRLMFGRRYSDGLHQ
                     AIEAKEGVKVERESQTLATITFQNYFRMYGKLAGMTGTAKTEENEFLAIYNLDVVEIP
                     TNMPMIREDYPDAVYKNKRGKFNAVVEEIVRLHEKGQPVLVGTISVETSEMLSDMLKK
                     RGVPHEVLNAKHHEKEAQIVAQAGQLGMVTISTNMAGRGTDIVLGEGVKELGGLHILG
                     TERHESRRIDNQLRGRAGRQGDQGSSRFFISLEDDLMRLFGSEKIMKIVESLGMEEDM
                     TIEHPLLSKSIENAQKRVEGRNFDIRKHVLQYDNVMNKQREVIYSQRRQVLLGEDIRE
                     NIMEMIAEIIGDAVATYTIGSEFAEEWDIKGLMEYLHTIFLPKNAFAREELLNFTREE
                     LTEAITGKAVDIYEQKESQVDPQVMRELERVALLRSVDTKWIDHIDAMDQLREGISLR
                     AYGQKDPVQAYQIEGYDMFLELVKNIKEDTVRFIYHVNISNLPQREKVAEPVATNQEG
                     GVRKPVVKEDKIGRNDPCPCGSGKKYKKCCGMNK"
     gene            complement(8099..8410)
                     /locus_tag="PKI50_02225"
     CDS             complement(8099..8410)
                     /locus_tag="PKI50_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02566.1"
                     /translation="MPHNSYNNIKKYAAFIFMVFTVFSGSSEVFDSDINTFAVSRFVA
                     QNARLSLSRTHIQSSVQNNESLRKYPDAENIQACVSFTILICILNNIIMKNSIWKNAL
                     F"
     gene            complement(8631..9185)
                     /locus_tag="PKI50_02230"
     CDS             complement(8631..9185)
                     /locus_tag="PKI50_02230"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HNT02567.1"
                     /translation="MLVESVGTSLAVGKMRGGSFSNIKDASLEKWYFFVLGFMVEFAA
                     VYAASRNIGFFKDNILIVHFLSYLLLFIGIYFNRRTLSFRIVFIGVFLNFLVIMANGG
                     QMPVSAEAMADIGLIDNMIAIRDGEIITHTLMNGNTALKYLGDILVLAKPYPRPKIFS
                     IGDVFMALGIFMYIQEIMVKKAGK"
     gene            complement(9175..10452)
                     /locus_tag="PKI50_02235"
     CDS             complement(9175..10452)
                     /locus_tag="PKI50_02235"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNT02568.1"
                     /translation="MPIKLKAYVAAIVLLGTIFFVFTLNQAHEVDAVGLVIFTLLSVI
                     AESMVIVTQGQRALSVGFIIGLAAIVVFGVPEAAWITSIGVMLRVVRHEGKTYHPFNF
                     PIEKTLFNGANATISAGLAGLCYEALGAVPGTIDLNSSIAALIAVITAMVIYILVNAV
                     IMSILMHLITGEKFFSNFINNMAWVVKDYLAMAPFAVIMAMAYANYKVAGILIFFIPL
                     LFARYSFKMYIDMRSIYIDTVKSLSQAIEAKDPYTNGHSRRVGEYACKLAERLGLPPK
                     KVENLKIAAILHDIGKIGIDESILNKPGRLTTEEFDKIKQHPEIGVKIIKDIDFLKDA
                     SEIILSHHERYDGTGYPEGRKYKDIILEAQILSIADVFDALTSERPYRNAMTVEEALD
                     IIEKGKDIQFESKLADAFIRMIKDDKEMKKVAG"
     gene            complement(10668..11300)
                     /locus_tag="PKI50_02240"
     CDS             complement(10668..11300)
                     /locus_tag="PKI50_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02569.1"
                     /translation="MSNNKAILYNDIQSLICKLPDIINCSVICDKEGKIEEIHILAGA
                     GRNIKQMVRDIQSAISAKFNISVDYKKISIAQINEAEFKETRVRIEGIQVKNVNNAIE
                     AEVTLSYDDKVYQGRSSKVKSRSNRAKVVAEATLAALEACIGISGVLYLEGLENFKVS
                     DKEVFVSLVGFSKDNIDEYYSGSSIISLDENESAVKAVLAAVNRKVNTVA"
     gene            complement(11526..12056)
                     /gene="raiA"
                     /locus_tag="PKI50_02245"
     CDS             complement(11526..12056)
                     /gene="raiA"
                     /locus_tag="PKI50_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNT02570.1"
                     /translation="MRTTVSGKNIVITNALKERVEKKLSKFERYFGPDTEVQATLSVE
                     KNRHIIEVTIPFNGVILRGEEATGDMYTSIDNVVEKLERQMRRQKTKLEKRIKDGNIK
                     FENWAAPPAEFEDEEVKIVRTKRFAMKPMPVEEAVLQMELLGHSFFVFSNADTEEVNV
                     VYKRKDGNYGLIEPEF"
     gene            complement(12756..13043)
                     /locus_tag="PKI50_02250"
     CDS             complement(12756..13043)
                     /locus_tag="PKI50_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HNT02571.1"
                     /translation="MFEDKTLICKDCGNEFTFTAGEQEFYAEKGFQNEPARCKDCRMA
                     RKNASGRNGQRREVVMYDTVCADCGKETQVPFKPTNARPVYCKECFDAKRQ"
     gene            complement(13455..13937)
                     /locus_tag="PKI50_02255"
     CDS             complement(13455..13937)
                     /locus_tag="PKI50_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02572.1"
                     /translation="MDNTNSVIIELLSLTDKKKQLFDSILEITLAQKKDIEENAANKI
                     EGLVSKKQSVIDSIDEIDKSFSEKFKLLKKQLMVNSLEEVDFTKYPELKNLKLKVEEI
                     MSMAQEIMLIERSNKEKLVSIMNEMKREIKQIKAGKKSIKAYERPITNNDGIYVDKKK
                     "
     gene            complement(14005..14400)
                     /gene="fliS"
                     /locus_tag="PKI50_02260"
     CDS             complement(14005..14400)
                     /gene="fliS"
                     /locus_tag="PKI50_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNT02573.1"
                     /translation="MAMANNAHDIYKKNMVTTATPQELTLMLYNGLIRFLKQAVMGID
                     EKSIEKANNNIIKAQDILFEFMRTLDMSYEISNNLLVLYDYMNTRLVEANISKDKAIV
                     EEVIGYAEELRDTWAQAMKLAKQQAVVNR"
     gene            complement(14420..16360)
                     /gene="fliD"
                     /locus_tag="PKI50_02265"
     CDS             complement(14420..16360)
                     /gene="fliD"
                     /locus_tag="PKI50_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNT02574.1"
                     /translation="MASNINSVSTLRFSGIASGLDVDDMVEQLMKVERSKMDKVKQDK
                     QLLEWKRDDYRSITSLMRSFQSSYFDVISSTNMRSSTSYKAFSTTVSPSDAADAGAVS
                     AVATSAASEGTHSIIVGNLATAQVRQSAGGVTKDVQGDSGYTLTAGETFKLTIDGVTK
                     SITLSDLDGTDGVTLADLNKSIENAFGSGKVTVDDTTNPGKLTFKVSSETNGINKITV
                     SAGTTNNALANMGFGADAIYSNRLSSGDTLAVLKSKLDDSNGGLAFTALSDGTEGIKL
                     AINGKTFEFSETTTLYSMMNQINRDSTANVNMQYDEINDKFKFTAKQTGAGNNIEISE
                     SGSSFITTADISVNNAGEDAKITIDGTEITRSSNTFTADGITYTALKETGTREVTVST
                     KQDVDTVYNKIKSFVDKYNELISKINGELTEERDRDYAPLTDDQKEDMTEDEIKRWEE
                     KAKSGTLRGDSLLQKIVSDLRNTLYAGVEGESGTSFLFSIGIETGSWNNKGKLVINES
                     KLKAAIKDSPDKVAEIFSKESSIAYSADMTSTERSTRFKENGIINRMYDVIQDNIRTY
                     SNKNGQKGALLEKAGVVGETTENKNIMYSMIKTQSDMITTLTKKLQTREDYYYKQFTA
                     MEKAISQMNIQSVWLSQQYDSQ"
     gene            complement(16366..16749)
                     /locus_tag="PKI50_02270"
     CDS             complement(16366..16749)
                     /locus_tag="PKI50_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNT02575.1"
                     /translation="MKIAGNSISPAVSSDTMRNNAVKGNHAEISNRNNAVKSGEQEVS
                     AKPKDKSEINEYQFIRAVETANKALEGSFTSFEISVHEKTNRIMVKVVDKETKEVIRE
                     IPPEKILNLMAKLWEMAGIIVDERR"
     gene            complement(16841..17788)
                     /locus_tag="PKI50_02275"
     CDS             complement(16841..17788)
                     /locus_tag="PKI50_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT02576.1"
                     /translation="MNMLVTGGAGFIGRWTVKRLLEGGHKVWILDDLSNGSLDNIKEF
                     EGHKALMGFLKGDIKDNSILERLFENNFDICFHLAASINVQDSIDDPRTTFENDVAGT
                     FNVLEQCRKHNVKMVFMSTCMVYDMAENDQGINELHPVKPASPYAGSKIAGESLALSY
                     WHAYKLPVVVVRPFNTYGPFQKSGGEGGVVSIFIERKLKGQALNIYGDGKQTRDLLYV
                     EDCAEFLVMAGCSEKSNGEIINAGSGKDLSINTLAYMISGNYEQVRHVTHIHPQSEIQ
                     RLLCNYEKAEKLLNWSPRTGLQEGIEKTEAWLENNTIKL"
     gene            complement(17795..18940)
                     /gene="pseC"
                     /locus_tag="PKI50_02280"
     CDS             complement(17795..18940)
                     /gene="pseC"
                     /locus_tag="PKI50_02280"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HNT02577.1"
                     /translation="MRKFIPYGRQYVDEDDIQAVVEVLRSDFLTTGPKVDELENKITE
                     YTGAKYAAVLSNGTAALHAACFAAGIGPGDEVITSPMTFAATANCILYMGGKPVFADI
                     DPMTYNIDPWDIEKKITHRTKAIIPVHFTGQPCEMDEIIRIADNHGLTVIEDGAHAMG
                     AEYKGRKIGTIGTMTTFSFHPVKHITTGEGGAITTGDASLYEKLTLFRTHGITKNAEK
                     MTDYDGLWFCEQQYLGFNYRMTDIQAALGVSQMKKLDSFLEKRRSYAALYNKLLGTID
                     GLTIPYQNEYASSAWHLYIIKLNLSKLDIGRREVFDKLRDRNIGVQVHYIPVYYHPYY
                     KNMGYEKGLCPEAEALYEGIITLPLYPTMEIEDIYYIADNLRDILRS"
     gene            complement(18937..19929)
                     /gene="pseG"
                     /locus_tag="PKI50_02285"
     CDS             complement(18937..19929)
                     /gene="pseG"
                     /locus_tag="PKI50_02285"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HNT02578.1"
                     /translation="MKIVIRAEGGPGIGMGHVMRMLVLADKLKAFAEVVFACRDNKEF
                     EPGVRQIEACGYSVWRFSGETVIDGLAAIGGDCLITDSYDIDEGYFDRTKDIFGITGY
                     MDDINKVRIYADFIINQNIYAEDMEYKAGIGTKLFLGTRYLLLRDEFRNLPKRKTRMH
                     IEDVLITLGGADPGNLSETIALKLSKVFPEITFHIAVGPSFTNRDSIERIAGGNIILH
                     YNPKMSELMLKCDAAISACGSTVYELCACGTPIIGVAAAPNQIMTSRSMDIKGALKYA
                     AGAGEIVQQLESLNYNTRARMSDTARELVDGYGSIRVSEQIWKIICEKEKGGTV"
     gene            complement(19954..20988)
                     /gene="pseI"
                     /locus_tag="PKI50_02290"
     CDS             complement(19954..20988)
                     /gene="pseI"
                     /locus_tag="PKI50_02290"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HNT02579.1"
                     /translation="MNEIVLKNGRRIGEDNPCYIMTDIAANHNGDLETAKELIRKAAE
                     AGADAVKFQTYRAEDLYSKKTPQFSRDSMKPYDLIKKVQHPREWIPILSEHAKGFGID
                     FLSSPFDYDAVDLLDNTGVSLFKVASLEIVDLKLIKYIAGKGKPVVLSTGMASLGEIE
                     EAIEAARSTGNGDIALLHCNTCYPAPAHIVNLKAMETLRNTFRLPTGFSDHTLGWHIP
                     VAAVALGACIIEKHFTLSREQEGPDHGFSIEPEELKLMVRQIRDVEKAMGDGVKKVSR
                     EELENYEKGRRSLIAITDIPKGTKITESMITAKRPGYGIKPKYFDMVIGRTAKVDIFE
                     DDVLLWDMLV"
     gene            complement(21000..21719)
                     /locus_tag="PKI50_02295"
     CDS             complement(21000..21719)
                     /locus_tag="PKI50_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNT02580.1"
                     /translation="MVTCIVQARMGSERLPGKVLRKVLGIPLILLNLKRLEKSREIDR
                     MILATSDRQLDDPLYETVKGEGFEVFRGDEENVLKRYRDCVLEYGGDIVVRVTGDCPL
                     IDPDIADNVISHYKMYDYDYVRLDVPDTFVRGFDTEVFSREALFKAFDLADEYRYKEH
                     VTAYMYLHPELFRTGLVRGESLFKKNYRLCVDTLEDFTLVEKIYEHFGDIYVSAGDVV
                     KFLDNNPDIAAINSEVVQKHV"
     gene            complement(21739..22653)
                     /locus_tag="PKI50_02300"
     CDS             complement(21739..22653)
                     /locus_tag="PKI50_02300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT02581.1"
                     /translation="MEHRYEKHWEHKEYKIIECMDCGFKHLYPVPEKESMKEFYDEKY
                     YSEIKPFYYKNVTEDFILRKSEEVDNNKIYGAIYDKVGEYKRAYENVMVDIGSGNDLL
                     ALYFQNKEWEVCVVEPNIKAAEYLNSYNLNVINDYMENIKPEILKGSSFINVQFVLEH
                     IHNPAAFLEKAYRMLVPGGIIRICVPNDFSEGQMAFKEYHSEAYRWIVLPDHINYFNI
                     NSLSGLLSRTGFRELYRTTDFPLEILLMGGMNYYTEEEYKKQVGPFIYNFENSLKSTG
                     RKQLLDRFYEGLANIGMGRSIYMYAIKE"
     gene            complement(22670..23341)
                     /locus_tag="PKI50_02305"
     CDS             complement(22670..23341)
                     /locus_tag="PKI50_02305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT02582.1"
                     /translation="MKALVFDLDDTLYNEADFVNGAFMEVAQYLSKKHCIFSHELYHA
                     MLKLLQEKGRGRIFNDICDLYNLDEDINNLIKIYRNAVPPISLYEDAEYFLKYCQGKY
                     KTGLITDGLYYVQRNKIRLLGIEKYFDSIIVTDEHGEGFCKPDIKPYLKMAENLGVAL
                     DSMIYVGDNPRKDFCGARQLGVYTVRIIRPAGDHMGLRLGSDYEADREITNMYELKAV
                     IDTLK"
     gene            complement(23338..24258)
                     /locus_tag="PKI50_02310"
     CDS             complement(23338..24258)
                     /locus_tag="PKI50_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT02583.1"
                     /translation="MKLLITAVGKRVQLIKYLKRQFTVIGADCSMLAPASFHVDRFCP
                     VSPCDNPGYIGEVLNICRKYGIDILLPLYEKEFYLLDSIREELLNEGTLLLLSDKRVL
                     DICSDKWETYQFFIGSNISTPISFKNKAECNIEFPMFIKPRRGMGSFNSYELNDMEEF
                     DFYYERIPEPIVQEKLSGVEYTMDCLSDLGGKVISVVPRERLEVRAGEVTKTRTVKDM
                     EIIEKTRYLLECLGSAGPTTVQCFRTVEGDIKFTEVNVRVGGGVPLTMEAGIDYGKIF
                     IDLIEGNKPEILLGNFKELTMLRYDEAVFI"
     gene            complement(24273..24842)
                     /gene="rfbC"
                     /locus_tag="PKI50_02315"
     CDS             complement(24273..24842)
                     /gene="rfbC"
                     /locus_tag="PKI50_02315"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNT02584.1"
                     /translation="MGRFIFSKTHIRGLYIIEPAVFEDGRGYFMETYNYNDFKEAGLD
                     MIFVQDNQSSSGKGVLRGLHLQKERPQGKLVRVLHGEVFDAAVDLRAGSDTFGSCYGT
                     VLSGENRKQLYIPEGFAHGFLVLSDTAEFAYKCTDFYYPEYEKGIIWNDPDIAIDWPT
                     DKVEGIILSDKDKRLPLLKSILDSFEPKQ"
     gene            complement(24870..25925)
                     /gene="rfbB"
                     /locus_tag="PKI50_02320"
     CDS             complement(24870..25925)
                     /gene="rfbB"
                     /locus_tag="PKI50_02320"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNT02585.1"
                     /translation="MKTYLITGGAGFIGSNFIQYMLNKYSDIYIINYDKLTYAGNPEN
                     LKSIEKDSRYNFVQGDICDKEKLSELFDKHDIDYVVNFAAESHVDRSIKDPEIFIRTN
                     VLGTVNLLNGAKADWSEGQGFRRGKKFLQVSTDEVYGSLGDTGYFTENTPLNPHSPYS
                     SSKAGADLVVKAYYDTFGMPINITRCSNNYGPYQFPEKLIPLIINNCLNKKSLPVYGD
                     GLNVRDWLYVEDHCKAIDNVIKSGEPGEVYNIGGSNERTNIHIVKYIINYLHDNVDKE
                     IDEGLIKYVEDRKGHDRRYGIDATKIGEELGWAPETGFEDGIVKTIEWYLDNIEWMSN
                     ITSGEYRKYYSSMYENA"
     gene            complement(25922..26803)
                     /gene="rfbD"
                     /locus_tag="PKI50_02325"
     CDS             complement(25922..26803)
                     /gene="rfbD"
                     /locus_tag="PKI50_02325"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNT02586.1"
                     /translation="MKMLITGAAGQLGSELLRIAKEMNCSLGAIDRSYNNPDIMALGA
                     KELDIADLPAVRNIVGRFRPDVIINTAAYTNVDGCEKEAEAAFKVNALGARNVAIAAQ
                     ETNSKLIHISTDYVFPGGGSTPYREYDKTSPKSIYGKTKLLGEEYVREFCKKYFIIRT
                     SWLYGRNGRNFVRTILSEAKEKQYLEVVDDQKGCPTNAEDLAYHILKIAVTDEYGVYH
                     CSGNGECSWYEFAIKILEIAGIDCPVKPIKTNQLDRAAERPAYSVMDNMMLRLTVGDD
                     MRSWEAAIEHFIKGLLI"
     gene            complement(26824..27597)
                     /locus_tag="PKI50_02330"
     CDS             complement(26824..27597)
                     /locus_tag="PKI50_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020756465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT02587.1"
                     /translation="MKGIILAGGTGSRLYPLTKVTNKHLLPVGKHPMIDHLIAKIKQA
                     GICDIMVITGKEHMGAVVNLLGSGHDYGVKFTYRIQDQPGGIAQALGLCEEFVHDDRC
                     LVILGDNIFEDDISEYVENFMQQEKGAKILIKKVPDPQRYGVAELKGGNIVGIEEKPE
                     KPKSDCCVTGIYMYDNRVFNVIKSLKPSNRGELEITDVNNWYIKDGSLTFDVLEGWWT
                     DAGTFSSFAYANELAKDKDLSGILCRDYEILREAAITKE"
     gene            complement(27630..28787)
                     /locus_tag="PKI50_02335"
     CDS             complement(27630..28787)
                     /locus_tag="PKI50_02335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT02588.1"
                     /translation="MNILHITTFLQGGAGRIICDLAIEQKKLGHNVYVVTSKTQESDY
                     CNYAEYINRLNNADVILYEIDSTFKRDLYLNLKVVEKVKEIIINSDIDLIHAHAAIPA
                     LVGLISRQASHKYIPVIHSMQGWGTNKTPEQEETDKIIMNGLDAIIASSQSSFDLMCD
                     KGINKEKIGLIYNGIACTDISESDKVDDDVREMIKLKHEGVKTIGCIGTICKRKNQGL
                     LLEAFKEVVKAEDAICVFVGEGELVHELMQKANEYGIADRVRFWGYKENSYQFYKYFY
                     CVVLPSLSEGQPITTVECFREKVLFIGSDIPSITELIKHGETGFLFKSNNPASLAGVI
                     KQVLSLTDDESAVIAEKAYDFYSSSFTLNAMLDNYMRLYEALILKGKGLHK"
     gene            complement(28787..29701)
                     /locus_tag="PKI50_02340"
     CDS             complement(28787..29701)
                     /locus_tag="PKI50_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT02589.1"
                     /translation="MNILVIGGQGFIGHNLVLKLVSEGHKVTVFEKNFKPERKLDSCD
                     YTIGSFSEIAQYENVLEGMDIVYHLISTTIPKTSMDNPSFDIQSNVVDTIKLLELCCK
                     HKVRKIIFPSSGGTIYGLAETIPISEDHPTNPICSYGITKLSIEKYLHMFNHLYGLDY
                     QVFRISNPYGPFQNPFSNQGAVGVFLGKILRGEEIEIWGDGSVCRDYIFISDVVQALY
                     LASEMHTDIKVLNIGSGKGVTLNDLVDIISRVTSKEAKVVNKESREIDLPVNVLDISK
                     AADKLMWFPQIPLEKGIEITWDWLMRYK"
     gene            complement(29862..31364)
                     /locus_tag="PKI50_02345"
     CDS             complement(29862..31364)
                     /locus_tag="PKI50_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT02590.1"
                     /translation="MKKSIILFGASKSGEAAMKKYTKEYKIAYFCDNDKSKWGTSFRG
                     VEVVSPDNLKMLDFDKIIITSMYYDEISKQLSNMGIEKYEVFKLSAKERSNKSLREYI
                     TSMHCKIQYIYEVSRSAYWDEQQRKWKQKLDFNKMAEELSKQLRAAGLDKPVLYDVSI
                     KNDILKERPKIIHAIPNTFVGGSTQLIVDLIEHLGHKYDMEIITAALPKEGMHKGMTI
                     HDFSGAKSGEEVSEFLKSKNADLLHIHYWGDTWYEHIFDSLNTYDCKVVENINTPIAA
                     YAHNKVDEYVYVSEYARRMFGDFPENSSVIHPGIDLSHFEPSKDNKRPYAVGMVYRLE
                     QDKLREDSIKVFIELAKRKPELLIYIVGYGTFFNSYVRQVYKENVRENFVFAGYVPYK
                     ELPAIYDKFSIFVAPVWKESFGQVTPFAMNKEMVVTGFNIGALPEILDGEVTLGNSLE
                     ELVERTIYYLDNQEAYRAKGSELRRRAQDCFCVKAMVSKYEQIYDKLLSM"
     gene            complement(31423..32364)
                     /locus_tag="PKI50_02350"
     CDS             complement(31423..32364)
                     /locus_tag="PKI50_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT02591.1"
                     /translation="MSNKRILVTGGAGFIGSHLCERLLKDGNHVICLDNFFTGSKRNI
                     AQLMNSKDFELITGDVENPIDIEADEIYNLACPASPVHYQYDKVKTLKTNVIGAMNML
                     ELAKKYDAKILQASTSEVYGDPMIDIQDEGYRGNVNPIGIRACYDEGKRCAETLFFDY
                     MRQYNVDIKVVRIFNTYGPKMHPEDGRVVSNFIVQALKGENISIYGDGSQTRSFCYVD
                     DLLEGLISMMNTSKDINGPINLGNPEEYNMLELAEKVIKLTGSSSKLIFKELPEDDPK
                     KRKPDISLAESVLGWKPSIAVEDGLIRTIGYFSELIR"
     gene            complement(32354..33694)
                     /locus_tag="PKI50_02355"
     CDS             complement(32354..33694)
                     /locus_tag="PKI50_02355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNT02592.1"
                     /translation="MNICVIGTGYVGLVTGTCFAEMGNKVWCVDVDNAKIEMLNKGIS
                     PIYEPGLKEMIERNSKNGRLKFTTDIKEGIESSLYCFIAVGTPPGEDGSSDITNVLKV
                     AGAIGQNMNKYTIIINKSTVPVGTGEKTKELVREELRLRNELNIDFDVVSNPEFLKEG
                     AAIEDFMRPDRIIIGTDNPRTAELMKQLYEPFVRNQHPILIMDIKSAELTKYAANSML
                     AARISFMNEIARLCDITGADVNNVRVGMGMDSRIGMAFLYAGTGYGGSCFPKDIKELI
                     SLGSRNGLEMKIAKAVEEVNESQKHYLVNMVTKRFGCDLKDMSFGIWGLAFKPQTDDM
                     REAPSLVIMKELTGMGANIIAYDPEAIEQSKCIMSEDEYNIKYVYDMMEAIDKVDALI
                     LVTEWRQFRQPDFREMKSRMKQSIVFDGRNQYDTIAMQENGWEYYCIGRNTNVK"
     gene            complement(33744..34808)
                     /locus_tag="PKI50_02360"
     CDS             complement(33744..34808)
                     /locus_tag="PKI50_02360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT02593.1"
                     /translation="MSKNPFVSVVINNYNYSEFISEAIESVLKQTYKNFELIIVDDGS
                     TDNSVSIIEQYHKQNQRIITPVYKKNGGQASAMNTGYNMAGGEIIALLDSDDYWFPEK
                     LERIVKCHENYDIVQHNLLINGEQKYINLRNDVDWSTALKKYGYLLGYWVPTSGLSFS
                     KALLGKVFPIPEVSLRLCADAYIIWNALYYSKIFSIDEALGFYRVHGNNNWHNKFNDE
                     RIKNTIDTILDILNEKLILSGESETIQYNSTLYKDLLGLFIEKDKKYVLYGTGSLSEE
                     FTRIVEQCESEIVYYSDSNSDKWGKHFMGREIIPPEDLLSKREHFDKIVIASSFIDEI
                     LIVLEKLGFVKASDIIVPQL"
     gene            complement(34835..36517)
                     /locus_tag="PKI50_02365"
     CDS             complement(34835..36517)
                     /locus_tag="PKI50_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNT02594.1"
                     /translation="MVRSVLIITKCIRMDAEGAKLIKRIKGLIENNISVIVASHGGNG
                     AAEIAEAGAEYYRFRWLNSEPDELDHKTFVENLSIIKGIIDKKQIDIIEVTDLYPALF
                     VYYLSKLVSIPYLVNFLSIEDLLADDFKYIGFVKEINSASILYAADSKLISEVQSHRE
                     ITFSNWKVIHAIDSDLRNLINEYNWVIDNFSTNDSIFLNYDDLHVTSNNSYLRFLEDK
                     DKSLVLFGASEFGLYAQDSLRMNNIMVDYFCDNDVSKCGSIISGAAIISPDQLLALYG
                     RVNILITSMYRAEIERQLLHLGFSESDIYFIPREIYGRLSYYKKSQEAISCSCERIAE
                     ALDILEDIKSKQVFINIIKHKMSFDFNLINEIIDHEQYFDSSILEFCDHETFVDAGAY
                     DGQTTMEFIAKVKGRFNKAFVFEPDKNMLDKVAGTIDSVNKDNNIKLIAGGVYDKSGV
                     IKFTPGEIGGSHISNNGSENIEIYKLDDVLKDEDVTFIKMDIEGAEMNALIGAQNIIR
                     ENKPKLAICTYHMPPQDFWEIPLYIKTLVPEYKIFIRHYGYDVRDTICYAVI"
     gene            complement(36555..37991)
                     /locus_tag="PKI50_02370"
     CDS             complement(36555..37991)
                     /locus_tag="PKI50_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT02595.1"
                     /translation="MIFCTSVCANYLPKAMVLAESLKKHNPNSEFVVCLVEKTIEEAA
                     EKFEYFDYVVLSKDIGIRDFDNFIFRHTIVEASTAVKGDLFEYLLNNFKDEDKFVYLD
                     PDIMVYSSFSELDSILEKNEIVLTPHTYFPEAGEEFNNGIFLECNHLKRGVYNLGFLG
                     IRRGEESSKFITWWKNRLNLLCYDEVDKGLFVDQKWIDLAPALFNVYILKEPGYNLAY
                     WNLSKRNISIQEGKDYYANDKPLVFVHFSGWDSGFNNTAIATYAPNVNELIHEINKQY
                     NAQLSEMDKYNLSKAKWSYNYFDNGDEIPRGIRIIYRNSGVLQQLYTHPFGQLNGELG
                     EYLEGLYNIKDNNPEADKWSIIDNYINEVCLLDKEWKAKKRIELGILKLILGGVEKKC
                     IIWGASNGGKIMKKVLDVFYEDVKIAGFIDKYKTGEFEGYNIYKPGQLKELSFDYAFI
                     ATSPGKADAEKYLKSNGCLPYKDYYSLF"
     gene            complement(38014..38742)
                     /gene="kdsB"
                     /locus_tag="PKI50_02375"
     CDS             complement(38014..38742)
                     /gene="kdsB"
                     /locus_tag="PKI50_02375"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNT02596.1"
                     /translation="MKILAVIPARYNSQRLPGKPLALIGSKPMIQRVYEGTKQCEFFN
                     KVIVATDDDRIKECVESFEGVAMLTDTNHSNGTERVAEVAQRFPEADIIVNIQGDQPF
                     ITSDILTELIHPYLNNQMPDMVTLACPLNEELDYTNPNVVKVICSTSNKALYFSRAAI
                     PYYRTKLEAPVYQHLGLYAFTREFLLKYVRLEPTPLEQCEGLEQLRALEHGYNIVVRI
                     IKESIMEINTPEDLVRTELKKKFI"
     gene            complement(38742..39725)
                     /locus_tag="PKI50_02380"
     CDS             complement(38742..39725)
                     /locus_tag="PKI50_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNT02597.1"
                     /translation="MNNGLYEGNIRRVIQQEGEKLLQIADNLDYEKVNIAVELLLNCK
                     GKILLMGVGKSGFVARKIAATFTSIGTPAIYLNATDAMHGDIGIVSSSDIAIVISNSG
                     ESHEIIEVLPFIKNRNVSIIALVSNAKSTLAENADIVIDLMVKQEACQYNLVPTTSAA
                     AVTAFGDALALTLMEAKNIGPENYALNHPAGRLGKRLTLRVKDLMHVKESNPTIYGDS
                     NLYEIIKAISDGGLGAVSVIAEDGHLIGIVTDGDIRRAVQNNLPNNIYDLYAEQVMTK
                     DPIKVTQDILAFEAMNIMENRISQISVLPVVDNNNVSIGMIRLHDIVRSGL"
     gene            complement(39727..41427)
                     /locus_tag="PKI50_02385"
     CDS             complement(39727..41427)
                     /locus_tag="PKI50_02385"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HNT02598.1"
                     /translation="MEFDYFNLFNKVKNIIYEAQAKKLVIFGTGSFAKLLSEYMPKKP
                     SYYVDNNINKLNRLFMDSDIHSPDLLKTEDSNNLLILVASMYYEEISQQLKSMGLNEG
                     EHFENGRPLYDLINVYKSFGLEEDLKEYIRKEQLITSSCTDPHKVVLANKECEWLCAD
                     NDRLYLKGLLEGYMLVFVEDNRNTHNIYFEKMDKSFRQIEYKGINLYEVSIYNICVDL
                     QLVITDIDISLNEHKDAIRKWYNISANYINTIENLTESLNVTSVMILQGYLYTSAILR
                     RFALSNEMPILALENTFNKTKAIWDNISGITVNKNLARSYYWRYYDSVDFSTAFEYCM
                     KYIDGIKNLKMEEHITPSNKFLQKSGRKTILYIGQVYSDSSVLFGIWNFKNPVEIIKV
                     LVDYCINNDFQLIVKPHPKEKDLVDVLGNKYDKLTFRMINKDKELAEKITKYGVVVDS
                     ENEYDTYSLIDYSDLCITINSQAGLEALIKNKDVILCGNCSYGGLGFSYEAHNKDMLL
                     CFMDMVLKKGISLINKEDIYKFFYIFNEKYCISKDAEAIIDKLKTIEFGLNKKNAGRN
                     "
     gene            complement(41459..44485)
                     /locus_tag="PKI50_02390"
     CDS             complement(41459..44485)
                     /locus_tag="PKI50_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNT02599.1"
                     /translation="MDNNVLLSTLKYNYCIGCGICRMSCPYEAIKMVLTKNYDINPKI
                     DKSKCIKCGICTEYCPHTKEKLLSEIQKVCETSSPNSFGLEKATYLLAHDKDADKRKK
                     SASGGVATALVEHLFKKKIIDGVIHATMRLGSIGELHYEASISNSFVEADRKRSSYYG
                     QIDYSHIVDEIKGKEKTYLVIGVPCAIRALTKLFKEHSSYNKNKIYTLALACSHNVNG
                     QFVDFLAESLGIPKDIQFKANLRNKDNIKDANNFNNHFFNGQQDIVKINRFKSLFTDI
                     WRNYFFSMKVCGYCSDFWGYTADISLKDAWGKWADNPLGDSIAVIRNKKLLDELLDSD
                     NIEYKYLDFETVAGSQLSTTLYKQAKAADRLNKPIWHIDNIRSGYLKHTVLSELSKFC
                     YRHFGYELSKRILFIVLAVLVKLENNARNIENIGFLKRKALIKDITKKADNKRVVFFG
                     TGKMAETLKKAIPVHVEYYVDNDEKKHNTMIDNTIIKDPNVILYENRDDVLIIVASMY
                     IDQISMQLEKMGLIPGVHFIDGRILFENIAGKPKHFKESKFAMGIEVILKPFIAVFIK
                     AKSIFVYEKTKKHKKILVVGGYGYKNTGDEAQLSATIRSLKEEFEGYIIKVLTPNQHY
                     TYFTHGECNVGDAPRVAFYDAGETALYYLDSTYKKLKFIFVSLLVYWNAFFVRAGLPL
                     FLINAKKAALLYEISTSDMIYFSGGGYLTGKTVSRLWDSMFLIRIADIFRVPVFLSGQ
                     TIGVWNGSFNKRLSKWGLSKAKLITLRDSYESVRALEEIGISGEHVFSTFDDALFCEK
                     LEDESKINNLLMNSGIRDEQANKGYVAINIHYWGLDTSEEKRQLLEKIDKVVGIVLRK
                     TDCNILFVPMVPSDEEAINDFMKAYRKDRLYNLSYDYDFRTVRALIGGSDICVTMKHH
                     PIIFALGEKVPVISLALGDYYEHKNIGALGIFGLEKCNIILSDALYYRDFDLILDEIY
                     SAKENIIRKIEESYKEVRQKKAYFISRIKEIISNKL"
     gene            complement(44518..45420)
                     /locus_tag="PKI50_02395"
     CDS             complement(44518..45420)
                     /locus_tag="PKI50_02395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT02600.1"
                     /translation="MKKCDFCGSIMNFIYNMNNYEIVQCDKCLTSTVLSMPQDDEIED
                     FYSGFMFCANASNKSIFEDETFNKWFDSFNLGCNAKMLDVGGGGGFFSYAFEYFDKGD
                     SYYIDIDNEACEFAAQELGLKNVINDNVKNLKDRTNLKFDFIYARHLIEHLKDPLEFI
                     DSCIELLTASGVLIIQFPNGVSFEYLGYPELLKSRVKTIQSSNRNFSRIETLLTICSN
                     KIAHGIDPIRHLWAITPKGISSYLSGKDVDFEIKNAPLTDPVYSPYYKAKGVYNKVRS
                     SIINNSLVKINGGTHLIVIIKRKQ"
     gene            complement(45455..45949)
                     /locus_tag="PKI50_02400"
     CDS             complement(45455..45949)
                     /locus_tag="PKI50_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNT02601.1"
                     /translation="MSRVKMLIMDVDGTLTDGKLYISGSGEEMKAFNVKDGYAIKNIL
                     PSMGIIPVIITGRKSKIVEIRAKELGILEVHQGIENKEETYIKLLDSYSCQDEDIAYM
                     GDDINDIPILKRAGISACPWDASAAVKSVCSYVMSSKGGEGAVSEFISHLHEEITGSK
                     LGVL"
     gene            complement(45949..46779)
                     /gene="kdsA"
                     /locus_tag="PKI50_02405"
     CDS             complement(45949..46779)
                     /gene="kdsA"
                     /locus_tag="PKI50_02405"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNT02602.1"
                     /translation="MQPRVVNVCDVKIGTNNPLVLIAGPCVIESEEIVMRTAEYISRL
                     AEKLRVSFIFKSSFDKANRSSITSYRGPGIDNGLKLLDKVKSSFRVPVITDIHSAEQA
                     MPVSDVADIIQIPAFLCRQTDLLTAAARTKKPVNIKKGQFMAPWDMKNVIDKILTYDN
                     TNILLTERGTSFGYNNLVVDMTSIVEMRKFGFPVIFDATHSVQKPGAMGTTSGGNCEY
                     VKYLAQAATAIGIDGLFLEVHPDPNTALSDGPNMIALNKLEELLVTIMELDRTIKGGI
                     "
     gene            complement(46815..47801)
                     /locus_tag="PKI50_02410"
     CDS             complement(46815..47801)
                     /locus_tag="PKI50_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02603.1"
                     /translation="MKNFFIKHKDKILSGQKVLKEYDIFEEIDTRLSDSYLFDRENNC
                     VKLGYSRCKKIGFDNLLLYRFGTVSRDDETLIFVPQVDEQIGDFIKRSQVFLNNPVLP
                     DETPVIHLNFDIHEKIEACIWLGNKYAYETYDMYLKGIIPWDNERKRSTEFKRTGIFI
                     GTSSIHIIEYSGTEAEHKHMVFSTPNYGNEVDCYIDLKKGYFRLGQNGSKIYHSGLKQ
                     DESCSINVSCNSRDNQVFSCTNPGLILRRFELCRNQGVIYFKSIDASLAKESRVMMLY
                     TGDIAVQYFNEKSNQWHFVKDSILFKDLHNVSLRAIMDTDDRIYALILLKVI"
     gene            complement(47825..48799)
                     /locus_tag="PKI50_02415"
     CDS             complement(47825..48799)
                     /locus_tag="PKI50_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT02604.1"
                     /translation="MFENKKILVIGGTGTIGQALVNKLISYNPEVIRILSRDEFKQYQ
                     MQQQLEGYKNIRFLLGDVRDKERIYKAMHGIDIVFNLAALKHVPSCEYNPFEAVKTNV
                     LGTQNVIECAIENNVKNVIYASSDKAVSPTNTMGATKLLAERLIASADYWKGGKIPVF
                     AAVRFGNVIGSRGSVVPLWEEQIVGRKEMTVTDPNMTRFMISIGSAVNLTLRACEIAK
                     GGEIFVLKMPVIRLGDLAEVVLDKVCSEKGIAKDEVNTRVVGLRPGEKMYEELMTVDE
                     AQYAVEFDDMFAIQHLSNITLPSNKPKEFRSDMCSIVEKSSIWSMLID"
     gene            complement(48936..50822)
                     /locus_tag="PKI50_02420"
     CDS             complement(48936..50822)
                     /locus_tag="PKI50_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNT02605.1"
                     /translation="MGTDNSAILRTRFESLWESLNKTTENVGPNVVIENARNSMYTLY
                     VENSGNKLYIHSRYNPVNEAEMFAGQFKDVDKYDHVIFYGIGMAYHIEAFFSKFGNKH
                     FSIYEPFLNIFKSFISYRSMDSIPLEYLDNIFIGDSLEDPYECLEALLKEIEGEVLLV
                     ILPSYERIFSQEYKSFYQRFGSILDDTKTLNRTNRLFEKLYVTNIMKNFMEIIDSPNI
                     LHLKNNKFAGNPAILVAAGPSLEYELKNLRYIKKKGLAYVLSVGSAINTLLEYDIYPD
                     AALTYDPQNHNHIVFKKLKDKGITSIPVVFGTIAGYGTVQAYEGPKLHFISSNDHVAP
                     YFFKPESAKEFDTVHDAGSIAVAAMQMLYKLGCDPIILVGQNLAYKDRKLYAKGIDYG
                     RDLSETDNKYLDSSFMVDDVEGNKIQTNKVLNHYKDEFERCIKQYGIENIINTTRGGA
                     AIGGTVYKPLKDVMENMLVSSIVDAEWYKDEGVSYDIDYMISRCKSIEAEQRHLLDIL
                     KSIKKIAAQINGNTNSVEAPESLFSFFYAEFNKMLQNTYFSTFIYPMNYYQCSLLNRK
                     INSINTEKNIVLKIQKLCREMEPFIDGCINDMHLISPLFGDMYEAIMDNDIKIMTFTI
                     KLCS"
     gene            complement(50809..51438)
                     /locus_tag="PKI50_02425"
     CDS             complement(50809..51438)
                     /locus_tag="PKI50_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02606.1"
                     /translation="MQVWFLDRTYNYSNESASVKELFDNIDRLLADPQYILAGMIIDD
                     MEIYENYVEYITKRIDRIKTIKVVIDTVENTIDSILESMSKYMDRAVPELELLAGSFY
                     SGGDEEVSIEKLGQLAEGIQWILNMIYYIDNNKALHSVLKSNSIWNECSSYGNRLGEE
                     FNNLKEAMGNIDMILIADILRYEITEVFTGINQRIKQIYGHGDEGNGNR"
     gene            complement(51570..52337)
                     /locus_tag="PKI50_02430"
     CDS             complement(51570..52337)
                     /locus_tag="PKI50_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNT02607.1"
                     /translation="MRINNNIMAMNAQRQLGVNSTGQSKALEKLSSGLRINRAADDAA
                     GLSISEKMRGQIRGLAQAQRNAEDGISLIQTAEGALNEVSDMLVRMKELGVQKLNGTY
                     SAGDVANMTAELTRLNSAITSIVADTKFNGTQLLNGGTVNLMLKDTGTATAYNLANIG
                     TVPAADADLATIETAINTVSTQRANLGAVQNELEHVVKNLGTTTENLQASESRIRDTD
                     MAKEMMAFTKLNILNQAAISMLAQANQAPQGVLQLLQ"
     gene            complement(52500..52727)
                     /gene="csrA"
                     /locus_tag="PKI50_02435"
     CDS             complement(52500..52727)
                     /gene="csrA"
                     /locus_tag="PKI50_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNT02608.1"
                     /translation="MLVLTRKKDEAVVIGDDIEIIISEISEDKVKIAIKAPKNIKIFR
                     KELIKAVEDENRKSVDMSDLDIDGLREAIKK"
     gene            complement(52727..53158)
                     /gene="fliW"
                     /locus_tag="PKI50_02440"
     CDS             complement(52727..53158)
                     /gene="fliW"
                     /locus_tag="PKI50_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNT02609.1"
                     /translation="MIIKTKFFDEIEISDKDIVDFDEGIPGFPGTHRYAIINDDNTFI
                     SYLQSLDSEDVCFIMIPPVFALKDYDIEISESTVKKLSIERPEDVKLYAILTILGNFK
                     DATANLKAPIVVNVNNNKAVQEILDDERYGIRHRIVKESDV"
     gene            complement(53187..54452)
                     /gene="flgL"
                     /locus_tag="PKI50_02445"
     CDS             complement(53187..54452)
                     /gene="flgL"
                     /locus_tag="PKI50_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNT02610.1"
                     /translation="MRVTNSMIINNLMRNLNKNLNRMEKTQRSLSSGKKFVNPSDDPI
                     GVSRSLRLNTEVATMNQYKRNADDVLSWLSTTEMAASNITEVLKRAKELTVQAASETN
                     SIDERVSISKEIEELRNQLIQIGNTTYAGSYLFSGFKTDKPLLAKDGTYDLGDSTNIL
                     SSEEIIEVNIGVGDRIGENFVGQRLFGLYSVAADLDLKNAESIKQQQIITGSFLDLAT
                     NPIDTATSFDITYQGATTTINLPNVPYNDIADVEGDISAAIMASADLNGHVTVSVANN
                     KIVFEADGEFSVQTGTAIGISDNKKSVSKVSSGDKSQMIAIFDQLLADLIADDTAGIG
                     NALNRIENQFENVNAITAEIGVRMNRVELTSNRILDDTLNLKDLLSKNEDADIAEVVM
                     NLKMEEYVYQASLSGGAKIIQPSLVDFLR"
     gene            complement(54465..55931)
                     /gene="flgK"
                     /locus_tag="PKI50_02450"
     CDS             complement(54465..55931)
                     /gene="flgK"
                     /locus_tag="PKI50_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNT02611.1"
                     /translation="MRTTFGSFNIATSGLFASQRSLDTTSHNISNANTDGYSRQISKQ
                     RATMPTYGDPTGVVGTGVETYDIIRMRSSYLDTKYWGQNKTYQEWNVKQTQLENLEGL
                     FNEPSDTGIRIVMDEFFTALEELSKQPGDSTCRVNVVEKANMLTTTINRNGHELVNSI
                     RDVNFSIKNKVSQVNSLTEQIANLNKHIFSFELGGNKANDLRDQRTLLLDELSSIINI
                     TVIEMPGPNNNNYMDVKVGGITLIDHIHFNKLATEDETKEVSDLGGGKISKVVWEGLD
                     NQEVNISSGELRGLLDVRDGNGEDYSYRGIPYYLNKLNEYVKGFTTAFNTQHKMGVDL
                     QGDPGQNFFYEPGIPEVNCISFKVNSDIIADPTTIAASSQGNGESNNDNIKLLIALRE
                     NKGMFSSLIGTPDDFVKSILSALAVDSNQAKRMTTNAQALTDHIYNSRLSESGVSLDE
                     EMTNMVKFQQAYNASARMVTTLDAILDTTVNRLGLVGR"
     gene            complement(55944..57386)
                     /gene="flgK"
                     /locus_tag="PKI50_02455"
     CDS             complement(55944..57386)
                     /gene="flgK"
                     /locus_tag="PKI50_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNT02612.1"
                     /translation="MSFRGLSIGVSAVFANQRALDVTGHNISNVNTAGYTRQIISNSN
                     AFYQKLGHSGNGKVMQVGYGVDVQEIRQYRDEFLDNKYKKEKTGLGYWEERYSSVREL
                     ETIFSDNSEDGLQEVMNNFWNSWEQLSKPTGGLTTRAMVKENAIAFVETVKNMDTMLL
                     NFRKSKDKEIIDNAARINEIAKEFATLNLDIKKIESHGVTANDLRDRRNLLIDELSGL
                     AKIQIIEGDYINVSLEGRLLVEGSRYEQIKTVPDTGNSGFVRLVWEIDNGSLYISGGS
                     INSSFESRDVLVGGFRERLDQFVIGIADEINAVHITGYGISDTLQRPFFINYRDNSAV
                     GINLLTIAYNPELENYDRIAAAEDAYSNEDNRIALKIAALRYTDCFTDEKYTTTAADK
                     KYNYDEFYRNIISDLGNIGQEASTAADAQKLLVDQIDYSRQALSAVSLDEEMTNLIKY
                     EHSYNAATRIVNVIDEMIEMIVNRTGLVGR"
     gene            complement(57452..57964)
                     /locus_tag="PKI50_02460"
     CDS             complement(57452..57964)
                     /locus_tag="PKI50_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNT02613.1"
                     /translation="MINVDKEVNAVIDILAEEYGYYSDLVELSKSKKDIIVAGKVAEL
                     DKMVKLEQDMIFSIGQLERKREDVISMLCKALDMNSERVTVHELAKVLKPELKNKLED
                     IQSKLQKALSELKAANDINGQLIEQSLEYIDYSINLIAGSGMETGSLYEDIGKNKSKQ
                     GKKNIIDTKV"
     gene            complement(58026..58331)
                     /gene="flgM"
                     /locus_tag="PKI50_02465"
     CDS             complement(58026..58331)
                     /gene="flgM"
                     /locus_tag="PKI50_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNT02614.1"
                     /translation="MGFNINKIDGVSGVYKNQKVASRKDVPESTRARGKKDEVQISKE
                     AMDFQLVMKAAKAAREIPDIREEVVAPIKEKLDNGTYEVDSKGIADKLLARRLNLKI"
     gene            complement(58466..58891)
                     /locus_tag="PKI50_02470"
     CDS             complement(58466..58891)
                     /locus_tag="PKI50_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT02615.1"
                     /translation="MAEIRNCPRCGKIFTYIGKPICGRCIESEENEFKVVKEYIYDNP
                     GATISEVSQETEVSVEKIMRFLREERLEIKSENSNLMLECERCGRAIKTGRFCDYCKE
                     QINKDFKREFGLDRKEPEPRPQTRAEKERMYTATRRKND"
     gene            complement(58935..59639)
                     /locus_tag="PKI50_02475"
     CDS             complement(58935..59639)
                     /locus_tag="PKI50_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNT02616.1"
                     /translation="MIKYLLDLLYPPKPKCSICGRAGRLAVCNACMASLDYLQGITCL
                     HCGKQLNEQYDDCTCPDCKTGIFYYERAYSCFEYGGMGKKLIHKLKYEGMVQLANVIA
                     GLMAERLENERLVIDAIVPVPIHKKKLEARGFNQSQIIARELGERLHKPVMGCLERMV
                     ETKEQYNLDRNQRFLNIRGAFSVKLSYNIGKYKNILLVDDIYTTGSTVNECSKLLKDA
                     GAAKVYVITAATGSNT"
     gene            complement(59655..61859)
                     /locus_tag="PKI50_02480"
     CDS             complement(59655..61859)
                     /locus_tag="PKI50_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNT02617.1"
                     /translation="MDEVQGTICEIIFRNEYNGYTVLDLECNNELHTLIGYFSFLNIG
                     EIIKAYGSWVQHPSYGSQFKVETYTTVTPATINGIEKYLSSGMIPGIGPHTAKKLVEK
                     FGLDTLDIMQYNPDRLTEVEGIGDKKAAKISEAFQEQKELKDIMMFLEQYGIGPAYAV
                     RIYRTYGANTIKEIKENPYKLADDIFGIGFKMADRIAISMGVSKFSEYRTASGTRYVL
                     NQYHGNGHTFVPQEELVRSSAALLDVDEAHIEDTIVRLFIDNKLVAENIGDIRGVYSV
                     PFFRAESGTARRLMQLLYYKIPPMDLDMEGEIAEIEKAEGIELADKQKTAVKEALTQG
                     ILVITGGPGTGKTTTIKTIISIFEKHGLEVLLAAPTGRAAKRMQEATGREAKTIHRLL
                     EFGYGDSDEEMFFQKNEESPLECDVIIIDEVSMIDILLANNLLKAIAEGTRVILVGDV
                     DQLPSVGPGNVLRDIIDSGVLPVVRLDEIFRQAESSMIVINAHRINRGEPPVLSSKNG
                     DFFLIRQESQEDIVATIIELCTSRLPKYTGLDPYEGIQVLSPMKKGLCGVLNLNHVLQ
                     EVLNPRGSGKEEKQHREAVFRVGDKVMQIKNNYRMRWRNINNLETEGEGVFNGDLGTI
                     VSIDNEELYMEVVFDRERKVKYDFTMLDELEHAYALTVHKSQGSEFPVLVMPLTFGPP
                     MLMTRNLLYTALTRAKSMVVLVGKERFLYQMISNNHIITRHSGLRKRLSAAE"
     gene            complement(61864..63054)
                     /gene="metK"
                     /locus_tag="PKI50_02485"
     CDS             complement(61864..63054)
                     /gene="metK"
                     /locus_tag="PKI50_02485"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNT02618.1"
                     /translation="MLKKLFTSESVTEGHPDKICDQISDAVLDAILEQDSMGRVACEV
                     SVTTGLVLVMGEISTECYVDIPKIVRKTIEDIGYTRAKYGFDCDTCGVITSIDEQSSD
                     IALGVDKALEAKKGEMTEQDIAATGAGDQGMMFGFACDETPELMPMPISLAHKLALRL
                     SQVRKEGILDYLRPDGKTQVTVEYDDGRPVRVDTIVVSTQHSPEVDHDTIERDIIDNV
                     IKPVIPEGMLDKDTKFYINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSG
                     KDPTKVDRSASYAARYVAKNVVAAGLASKCEVQVAYAIGMAKPVSVMVETFGTGKLTD
                     DEIAKIIMKVFDLRPAAIIKDLDLRRPIYRQLAAYGHMGRTDIDVPWEKTDKVEELKK
                     LAGI"
     regulatory      complement(63140..63246)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(63350..63997)
                     /locus_tag="PKI50_02490"
     CDS             complement(63350..63997)
                     /locus_tag="PKI50_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02619.1"
                     /translation="MEFIPAKTIISGYTENNPWFGNNYSMNIYKGCCHGCIYCDSRSE
                     DLLNSIKRHSPVLVKHTVTTADDSLCSKIEPNAPAASRRFEAVRDLSMNGIFTGILLM
                     PVLPFLEDNEDNISNIIDLAYEKGAKFIYPAFGVTLRQNQREWFYSKIDEHFPSIRQK
                     YVKQYGNSYKCISPRAKELWKFFRARCDRLGILYKMADIINEYQQNYLYEQISMF"
     gene            complement(64052..64804)
                     /locus_tag="PKI50_02495"
     CDS             complement(64052..64804)
                     /locus_tag="PKI50_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT02620.1"
                     /translation="MARQARKRSATGVYHVMLRGIDKRDIFLHDEDKMIFMKKLLKAK
                     ENAKFGLYGYCLMDNHIHLLILESEEIGVSIKRVSVGYASWHNNRYGRVGHLFQNRYL
                     SEPVESEGYLVHVLRYIHQNPLKANMVKKPEDYPWSSYNDYISAYQGNGVNIDADMIM
                     AYFKDQNDFEEFTNTQNDDIFVDFEPEEKFTDEALGDIIKKKIGSVFLYGKSIGDRNR
                     LIKDIYNSTGASIRQLERVFGVNKNIIAKAVK"
     gene            complement(65013..65097)
                     /locus_tag="PKI50_02500"
     tRNA            complement(65013..65097)
                     /locus_tag="PKI50_02500"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65061..65063),aa:Tyr,seq:gta)
     gene            complement(65102..65177)
                     /locus_tag="PKI50_02505"
     tRNA            complement(65102..65177)
                     /locus_tag="PKI50_02505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65142..65144),aa:Thr,seq:tgt)
     gene            complement(65322..65396)
                     /locus_tag="PKI50_02510"
     tRNA            complement(65322..65396)
                     /locus_tag="PKI50_02510"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65361..65363),aa:Glu,seq:ttc)
     gene            complement(65411..65486)
                     /locus_tag="PKI50_02515"
     tRNA            complement(65411..65486)
                     /locus_tag="PKI50_02515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65451..65453),aa:Pro,seq:tgg)
     gene            65798..66352
                     /gene="yyaC"
                     /locus_tag="PKI50_02520"
     CDS             65798..66352
                     /gene="yyaC"
                     /locus_tag="PKI50_02520"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HNT02621.1"
                     /translation="MIEKYSIKDGICVHHTDNRAHLKISDFLKSNKLDKCLILCIGTD
                     RFIGDSLGPLTGTLLSKLSLPVPILGTLDNPIHAVNLRRVMSHIKTSYKDHKIIAIDA
                     CLGAGKSVGYVHVKEGSIQPGKGVGKRLSHVGDFSIIGVVDEAVESGDSEYENYLKIS
                     NVRLGLVMKMSETITKGIYAAFRQ"
     gene            complement(66413..66841)
                     /gene="fabZ"
                     /locus_tag="PKI50_02525"
     CDS             complement(66413..66841)
                     /gene="fabZ"
                     /locus_tag="PKI50_02525"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNT02622.1"
                     /translation="MLNNTDIQGILPHRYPFLLVDKITEIEYGKKAVGIKNVTVNEPF
                     FQGHFPGLPIMPGVLIVEAMAQVGAVAILGMEENKNKLAVFTGIDGMRFRRQVVPGDT
                     LRMEVEMLNFKRGIGKAKAQAYVGDELAASGELMFAIVNK"
     gene            complement(66831..67205)
                     /locus_tag="PKI50_02530"
     CDS             complement(66831..67205)
                     /locus_tag="PKI50_02530"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNT02623.1"
                     /translation="MINPINNNGITDYNNAKSKAQEAKQGEFGKALEKAMEEKDEKKL
                     KQACSDLEAIFVSMVFKQMRSTVQKAGLIDGGMAEEMYEEMLYDKYAEEASKGEGIGL
                     ADLLYQQLSKSLKPEREREDVE"
     gene            complement(67208..68002)
                     /gene="flgG"
                     /locus_tag="PKI50_02535"
     CDS             complement(67208..68002)
                     /gene="flgG"
                     /locus_tag="PKI50_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNT02624.1"
                     /translation="MMRALWTASTGMLAQQLNVDTISNNMANVNSVSYKKSRVEFKDL
                     LYETLERGIVRDGQGRPVNLQVGHGVMPAASVKFFTTGNLASTENPYDVAIEGLGFFK
                     VLGPEGQEYYTKDGAFKISPDGENFNLVTSEGYYVQGENGNIELGSDIKDIIIDARGL
                     ITVKREDGTIEELGSLSISTFVNPAGLESVGKNLYVATVASGEAFDTQNDGTGGNILQ
                     GYLEYSNVQIVEEMVNLIQAQRAYEMNSKSIQTADEMLQMANNLRR"
     gene            complement(68016..68789)
                     /gene="flgF"
                     /locus_tag="PKI50_02540"
     CDS             complement(68016..68789)
                     /gene="flgF"
                     /locus_tag="PKI50_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNT02625.1"
                     /translation="MIRGLYISASSAITEAKRVDVIANNIANVNTTGYKKDVMVTQNF
                     SEVLISRINDRFGKDLKVSRPFKPLGPLSYGIHSSEIMIDFKQGQLNSTGNSFDLALR
                     GKGFFCIDTPEGERYTRNGSFAKDAEGWLVTGEGWRVFGEDGYIRIEGSDMTVNEKGE
                     VFSDAQLAGKLRLVDFEDYEALRKEGNGLIRIAEEIEAEPVITAGTVQQGFLEGSNVN
                     SVKEMVEMLTMLRTYEANQRIIKIHDELIGKAVNEIARV"
     gene            complement(68963..69997)
                     /locus_tag="PKI50_02545"
     CDS             complement(68963..69997)
                     /locus_tag="PKI50_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT02626.1"
                     /translation="MWGGQDIGIDMGTASVLVYIKGKGIVLREPSVVAIDRNTNKILA
                     VGEEARLMLGRTPGNIVAIRPLREGVISDYDITEKMLRYFIDKAIGRRRIIKPRMVVC
                     VPSGVTEVEKRAVIDAANNAGARTTRLIEEPIASAIGAGLDISKASGNMVLDIGGGTT
                     DIAVISLGGIVVSTSIKIAGDKFDEAIVRYMRKKHNIMIGERTAEELKINAGTAFPRE
                     EQLKVEVRGRNLISGLPKTIEVTSDEMLEALSEPVSAIADAVHSVLERTPPELSADIS
                     DRGIVMAGGGSLLNGLDKLISKRTGIPVYVAEDAISCVAIGTGQALEHIDMLQDSDIN
                     ANRKIITLNY"
     gene            complement(70230..70484)
                     /gene="spoIIID"
                     /locus_tag="PKI50_02550"
     CDS             complement(70230..70484)
                     /gene="spoIIID"
                     /locus_tag="PKI50_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HNT02627.1"
                     /translation="MKDYIEERALEIAKYIISSKTTVRDAAKIFGVSKSTVHKDVTER
                     LPRINPSVALQVKEIMDQNKAERHIRGGRATKMKYQSISL"
     gene            complement(70593..71375)
                     /locus_tag="PKI50_02555"
     CDS             complement(70593..71375)
                     /locus_tag="PKI50_02555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT02628.1"
                     /translation="MKFRKEFNNRHIYFLVLLIAALLTAGGVMQYRNYLLDKQAQLDF
                     EKSMEEYIEESEPSSTAQVIEEKAESGGSENVVETPDATEVSNSSSVKITEDKKDTKE
                     KVNEAKPISAIKMEEPAMETMVVPVFGTMYTEFSEDVLVYSKTLDQWSTHAGIDIKAE
                     EGSPVRAAMDGVVDQVVNDPQLGLTIVLDHGGEVYTKYSNLSTLDLVSINQSVKKGDV
                     ISGVGKTALYEIADDPHLHFEVIKQGKSVDPKKYLPKQALKK"
     gene            complement(71442..72401)
                     /gene="spoIID"
                     /locus_tag="PKI50_02560"
     CDS             complement(71442..72401)
                     /gene="spoIID"
                     /locus_tag="PKI50_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HNT02629.1"
                     /translation="MKKLAAAIAVLMILLFAIPLLLVRDKGEESGKKEVPREDLPIEV
                     YMCDTDEVVTMNLEDYILGVVAGEMPASFHPEALKAQALAARTYTMLRMRQFGGSGCS
                     KHQGAEICTDSTHCQAYRDPASVKKDLDKLKDAVYGTAGEVIVYDNKLIDAVFHSTSG
                     GKTENSEDIWSNRVPYLRSVVSQYEEHSPKYVARQEISIDNFVQCIKKLDSGVVINKK
                     DIKNEIKVLERSEGGRITRIKIGNKTFKGTDVRNTLGLNSSNFNFDVTASTVLFTVVG
                     NGHGIGMSQYGADGMAKNGASYRDIVTHYYQGVAIVSIKDLTN"
     gene            complement(72841..74097)
                     /gene="murA"
                     /locus_tag="PKI50_02565"
     CDS             complement(72841..74097)
                     /gene="murA"
                     /locus_tag="PKI50_02565"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT02630.1"
                     /translation="MLTKLTIEDKCVLKGEVKVHSAKNAILPVIAASLLTPDTCVLDE
                     VPCLDDVDIMEEVVRSIGAEAERTNGSLVIKAENISSFEATYELVRKMRASFLIMGPM
                     LARIGKVKISLPGGCAIGTRPIDLHLKGFSLMGANITLGHGYIEAYAEKLKGETIYLD
                     FPSVGATENLMMAGCLAEGTTTIENAAEEPEIVDLASFLSKMGAVIKGAGTDTIRIEG
                     VEELKGTAHTAIPDRIEAGTYMVAAAATGGDVIVEGIVPEHLKPVTAKLKEMGAEVQE
                     YERAIRVRAAERLNSVDIKTMPYPGFPTDMQSQMMTAMCLAKGTSIATETVFENRFMH
                     INELKLMGANIKVDGRSAIIEGCEKFSGAKVRATDLRAGAALVIAGLCAEGRTEIHDV
                     YHLDRGYIRIEEKLKKLGADIRREEE"
     gene            complement(74107..74856)
                     /locus_tag="PKI50_02570"
     CDS             complement(74107..74856)
                     /locus_tag="PKI50_02570"
                     /inference="COORDINATES: protein motif:HMM:NF020264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HNT02631.1"
                     /translation="MRKKILLLIVCALFSVGSFEAAEKPFEALTEEEALVTSFRSTGA
                     DVLESTISCWTKLNDKFLDIEQIEAEMNRIVRRIEPDKVTVVKNDESDSGLNKIVVSC
                     FKGNRTYSIAIESIKQEGIGETYAVIDVSIDRSYQELIAERRNIIDAMQRPEGSMNFS
                     SCIIGTYKGRLDEKVADKKSRMALQSINAREVEGMENEELKSISAFSSSVGGHVVSDE
                     AKVNVQLAMRYSSYDDRTYIWIGTPLIPMGY"
     gene            complement(74973..75638)
                     /locus_tag="PKI50_02575"
     CDS             complement(74973..75638)
                     /locus_tag="PKI50_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNT02632.1"
                     /translation="MIQDKDRKREVIVKRVAQELKDGDLVNLGIGMPTLVANYIPEGI
                     DILLHSENGFIGLGPNPEPGKEDLDIVNAGGLPASVVPGGAFFDSAMSFTIIRGGHLD
                     VTVLGALQVDQEGNLANWMIPGKMVPGMGGAMDLVAGARKVIISMEHTAKGAPKILKK
                     CNLPLTAEKEVDLIVTDMAVIEVTPKGLLLTELAPETTLEEVISATEAELIISENLKV
                     MNV"
     gene            complement(75660..76304)
                     /locus_tag="PKI50_02580"
     CDS             complement(75660..76304)
                     /locus_tag="PKI50_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HNT02633.1"
                     /translation="MDKIRTIDEAASYIKDGMTVAVGGFIGAGTPEKLIDAVIAKGIK
                     DLTLICNDTALPDKGVGKWVVNRVVSRIIVSHIGTNPETGRQMNAGELEVELVPQGTL
                     AERVRAAGAGLGGILTPTGIGTVAEEGKEKITVNGKEYLLELPLKADVALLKGSIVDK
                     KGNIYYKKATRNFNPLMATAADLVIVEAEKIVEVGELDPTDVMTPSIFVDIIAK"
BASE COUNT        19975 a        18745 c        12298 g        25309 t
ORIGIN      
        1 atatatacgc actcgaaaaa aaattgtctg aaataactta attgtgttta tgacaattca
       61 ttccacatta ttatcgcatc ttcaccggta tctgcataat aaccctttct tattccactg
      121 tccttaaagc ccagcttttc ataaagcttc cttgcagcat catttgaagc tcttacctcc
      181 aatgtaatat gctccgcctt gttttccttt gccgtccgca gcatctcctt tacaagcttt
      241 tcccctattt tgcgctccct gaattccgga tgcactgcaa tatttgttat atgtgcctca
      301 tccagtataa tccacattcc tccataggct gcaaccttgc cgttcacttt ggccactata
      361 tacctggcaa gtttgttctt caaaagctct gattcaaagg attccctgga ccacggagaa
      421 gcaaagctca ggttctctat ttcaacaacc tggtcaatat catccaggct catgctttga
      481 aactctgctt tgttcttttt ctcatattcc gcttctgcct gggatttcct gatataggta
      541 agcctgaaag aattataggt atcaaaggaa cccttattat acatttccaa agccatcctt
      601 gcgcaggagg aagctcttgg cagcaagctg cctgcgggtg ccgcattata cccttccagg
      661 ccttttttca agttttcttc aaaaattacg gcaccatccc caaggaaggt tgtgggtaca
      721 gccttactct tcaccagctc tatcaaatcc tctatcggta ttaccccaag atcctccagc
      781 tttgtcagcc tgccggaatt atacgagtac aagctgctgt ataccatccc cctttgggca
      841 tccagcatcg ggcaaaccag gcctgtacaa ttgtaaaggt tgcaggccag tgcatcaaga
      901 gtcggaactc cgacaacagg cttatcgaaa gtctgagcca tagcctttat aattgatgct
      961 cctatacgca gtcctgtaaa agaacccggc ccggcagagc aggcatataa atctatttca
     1021 gcaggcttga tatcggactt gttgaatagc tcctcaatta tgcccattat cttctgggag
     1081 tgaactctgc tgatgttcaa tatgtattcg gatatcagtc tgtcatcttc cattattgcc
     1141 gctgttgcgg cggaagtgga cgtatcaaca gccagtattt tcattggatt ccattccttt
     1201 cattatttcc tcataagctt ctccggccgg ctccattatt atttcacggg aattgtctcc
     1261 ctttgtattc agtattgtaa tccaaagcct ctctcccggg attatatcac ttaccatatc
     1321 cgcccattcc aatactacca ctccgtcacc gaagaaatat tcctcatagc ctaattcgta
     1381 catttcttca acatcattaa gcctgtatac gtcaaagtgg tataaaggca ctctgccttc
     1441 atattcatta atgatggtat aggtggggct ggtaacataa tccttgactt ccagtcctct
     1501 tgctatacct tgggtaaaag cagtcttccc tgctccaagg tctcccgaga ggcacacaat
     1561 atccccgggg ctcagaagcc ttcccagccc ttcgccgatt ttggcagttt cttcagcgct
     1621 ctcagtatga aatttcagca ttgatatacc tccgtcgcga ttgctattga tattatactc
     1681 tgtttgaata taaatgtata catcggctaa ttcaagccct tcatgctgag gcttatcctg
     1741 ttcctgtcaa ggtccacatc caaaactttg accttcacta tatcacctac ctgcactaca
     1801 tcaagaggcc tcctgacgaa cctgtcagcc agctctgata tatgtacaag gccgtcctgg
     1861 tgtactccga tatccacaaa ggcaccgaag tcaataacat tccttacagt cccggtgaga
     1921 atcataccgg gcttcaagtc cttaatatcc agcacatccg acatcaatac aggcttcggc
     1981 atttcatccc tcgggtctct tccgggcttt tttagctcat tgattatatc ccttaatgtc
     2041 gggaccccca ctcccagctc agccgccagc tcatttacat ccactgctga aagcttatcc
     2101 accgcagcgg atatgtccct gcctgctttc tcaaggagct ttcctgcaac atcgtaggat
     2161 tcgggatgca ccgaagtgtt atccaatata ttttcacttt caggtatcct caaaaagcct
     2221 gctgcctgct caaaagcctt ttccccgagc tttttcacct taagcagctc tcttctgctc
     2281 ctgaacttgc cgttttcttc cctgtaggat actatattcc tcgcaatggc tgagtttatt
     2341 ccggatatat gctgcaggag agacggtgat gcggtattga gatccacccc tacactgttc
     2401 acgcagtctt ccacaactcc attcaaggaa tccgacagct tcttctgtgc aacgtcatgc
     2461 tggtattgcc ctaccccgat ggatttggga tctattttca ccaattctgc cagagggtcc
     2521 tgcagccttc ttgctattga aatcgcccct ctcagggata catttatatc ggggtattcc
     2581 tgtgatgcca gctccgatgc cgaatatacc gatgctccgg cttcattgac tattacatag
     2641 tgcaggggca tattcatttc cttgattatt tccgcgcata tcctttcgga ctcccttgat
     2701 gcagtcccgt tgcccaggga tattacttca acattatgct ttttaatcag cttcttaaga
     2761 atcattttgg cttcctccgc ctgattctgg ggcggagtgg gatataccgc agcagtttcc
     2821 agaagctttc cggtatcatc taccactgcg attttgcagc cggttctgta agccgggtca
     2881 aagcccataa ccacctttcc tttaataggc gcctgcaaca gaaggtttct gagattggcg
     2941 gcaaacacct ttatcgcctg ttcctccgct tcctcggtga gattattcct gatctccctt
     3001 tctatggagg gggcaataag cctgttgtat gcgtccttta ctgtttcctc aagcatattt
     3061 ttatagggag aattctcttt gattgtgctg ttgttcagat aagtcagtat cctgtctgcc
     3121 ggtgcctccg ctttgacatt caaaaattct tccttttccc ctctgttaat ggccagtatc
     3181 ctatgggggg caatcttttt gacaggctcg ctgaaatcat aatacatctg atattccgat
     3241 ttttcttcct tggtgccttt tacaacaata gtgccgttcc cgaaggtcag ctcccttatt
     3301 tcctttctga attccgcatt gtcggatatt atctccgcaa ttatatccaa agcgccgttt
     3361 atggcatcct gtactgtcga aacgccaagc tcgtcattaa cgaatgcctc tgcaatttcc
     3421 tccagagcct ttgattggcg ttcctggctg aatattatgt ccgccaaagg ctccagccct
     3481 ttttccctgg ctatggttgc ccttgtcctc ttcttttgct tatacggacg atagatgtcc
     3541 tccacttcct gcaatacctc agccttttca atggaagcct tcagctcctc ggtaagcttg
     3601 ccctgccctt cgataagcct taagacttct tcctttctgt tttcaagatt tctgagatat
     3661 gtaagcctct cacccaactc tctcagtgtt gtgtcggaaa gttctcccgt aagttccttc
     3721 ctgtaacggg caataaaagg tatggtattg ccgtcatcta ttagttttac cgtgttttca
     3781 acctgccagg gcttgaccga cagttccttt gcaagttttg caataatgtc catatattat
     3841 tctccttcac tctgtttttt catcctttat atattaacca tttttgtacg gatattgcaa
     3901 taggcgccga gtgtatgata gtatacaaaa taggtacata tgtatcccga ttgcatacag
     3961 aaagcgaggc aatatgtaat ggtaaacaaa aaaatgttca tagttgattc cgacatactc
     4021 cctaaagcat atgagaaggt tattgaagca aagctcctgc tcagaacggg agacgccgca
     4081 actatccagg aagcatgccg tatggcagga gtgagccgaa gtgcatttta taagtacaag
     4141 gacagcgtat ttgactatac tgcccaggaa cgggggcgta cagttacact tttcatgatt
     4201 ctcaagcata ttccgggggt gctttccgat ataataaaca aaatagcctc cgtcaagggt
     4261 aatataataa ccataaatca gaacattccg gtgatggact cagcttcact gacggcatcc
     4321 attgatacaa aaattatgga aataaaccct gaggaactgg tagagctgct tctatcctgc
     4381 aagggctgca aaaaggttga aataatagga atggagtaaa attatactcc attcccctgc
     4441 ttcaaatatg catttatgaa atcatccagt tctccgtcca ttaccgcccc tgtatttccc
     4501 gtttcttccc ccgtcctgtg atcctttacc aggttgtacg gatggaatac ataggagcgt
     4561 atctggcttc cccatgcaat ttcacggtac tcgcccttta tgtcgtctat tttatcttta
     4621 tgctcctgtt ccttaatttc cagcagcttg gcaaacagca ttttcatagc catttcctta
     4681 ttgctgtgct gggatcgttc attctggcac tggactatta ttcctgtagg tatatgggta
     4741 atccgtaccg ctgaatccgt tttattgacg tgctgcccgc cggctccgct ggatcggtag
     4801 gtgtcaatct tcaaatcctc ttcatctatc tctactttta cttcatcatc aagctcaggc
     4861 atcacgtcca ccgatgcaaa ggatgtatgc ctttttcccg cagtgtcaaa aggcgaaatc
     4921 cttacaagcc tgtgcactcc cttttccgat tttagatatc cgtaggcatt ttccccctct
     4981 ataagcaaag cggcactctt tatgcctgct tcataatccc ttagcatatc aaggattgtt
     5041 atagcataac ctttcctttc agcccatctt gtgtacatgc gaagcagcat ttcagcccaa
     5101 tcctgggctt ccagtcctcc tgccccggag tgtattgtga atatcgcatt atttctgtca
     5161 tactgcccct tgagcaatgt ctcaattctg aagctctcta catccttctg aagagtcttg
     5221 aaggatttct ccacctcagg tatcaccgat tcatcctgag cttccatccc cagctctatc
     5281 aatacttcaa tatcctcata ctcttttttc aggttttcta ttttttctgt tttatccttt
     5341 aaagccttta ttgttttcag aacctttcct gcattttcgg catcattcca aaaaccttcc
     5401 tggttcatct cctgctccag ttcgtatatc ctctcctgta tcgatgtcag gtcaaaggga
     5461 cacctcaatt tcctctattg catcccaaat ctcggataag ccgtatttat actgttcaag
     5521 ctccaacata tattattcct ccttatttat tcatcccgca gcatttcttg tacttcttgc
     5581 cgcttccgca ggggcagggg tcatttcttc ctattttgtc ctcctttact acaggctttc
     5641 tcactccgcc ctcttggttt gtggctacag gttcagctac cttttccctt tggggaaggt
     5701 ttgaaatatt tacatgataa atgaatctta ctgtatcttc ctttatattt tttacaagtt
     5761 ccaggaacat atcatatccc tcaatctggt atgcctgaac gggatccttc tgcccgtagg
     5821 ccctgaggga tattccctct ctcagctgat ccattgcgtc tatatgatcg atccactttg
     5881 tatcaacgga tctcagcagc gcaactctct ccagttcacg cattacctgg ggatcaacct
     5941 gtgactcctt ctgttcatat atatctacag ccttaccggt aatagcctca gtaagctctt
     6001 ccctggtgaa attcagcagt tcttccctgg caaaagcatt cttcggaagg aaaatcgtat
     6061 gcagatattc catcagccct ttaatatccc actcttccgc aaactcactg cctatagtat
     6121 aggtggcaac tgcatcccct attatttcgg caatcatttc catgatgttt tcccttatgt
     6181 cctcgccaag cagcacctgg cgcctctggc tgtatatgac ttctctctgc ttgttcatta
     6241 cattgtcgta ctgcagcaca tgcttcctta tatcaaagtt cctgccttcc accctttttt
     6301 gcgcgttttc tatggatttt gaaagcagcg gatgctcaat ggtcatatcc tcttccattc
     6361 ccagggattc aactattttc attatttttt cggaaccgaa gagcctcatc aaatcatctt
     6421 ccaaagatat aaagaacctg ctggaaccct ggtccccctg ccttccggca cgccccctca
     6481 gctgattgtc tatacggcgt gactcatgcc tttcggtacc cagtatatgg agccctccca
     6541 gttcctttac tccttctccc aatacaatat cagtacctct tcctgccata ttggttgaaa
     6601 tggtgaccat gccgagctgt cctgcctgag ctactatctg agcttctttt tcatgatgct
     6661 tggcattcaa tacctcatgg ggcactcccc tctttttcag catatcgctg agcatttcag
     6721 aggtttcaac agatatggtg cctaccagga caggctgccc cttttcatgg agccttacaa
     6781 tttcctcaac taccgcattg aatttacctc ttttgttctt atataccgca tcgggataat
     6841 cttctcttat cattggcatg tttgtaggta tttcaactac atccaggtta taaatggcca
     6901 ggaattcgtt ttcttcggtc tttgccgtcc ctgtcatgcc ggccagcttg ccgtacattc
     6961 tgaaatagtt ttgaaaggtt atggttgcaa gagtctggga ctctctctcc accttgacgc
     7021 cttcctttgc ctcaatggcc tgatgcaggc cgtcgctgta tcttctcccg aacataagcc
     7081 tgccggtaaa ttcatcgaca attattatct gcccatcctt aattacatag tcaatatcac
     7141 ggcgcatgag gttccttgcc ttaagtgcct ggtttatatg gtgctgtatc tccatattgt
     7201 tggcatcggc aagattttca aggttgaaga atctttcggc cttcgccacc ccttcctctg
     7261 tcaggactac ggtatgagcc ttttcatcta ttgtaaagtc agcttcattt ttaagccctc
     7321 tgacaaaagt atcagctaca taataaagct gtgtggattt ttctccgacc ccggaaatta
     7381 taagaggagt cctggcttca tctatcagaa tactgtccac ttcatccaca atcgcgtaat
     7441 tcagttctct ctgaaccatt tggtccctgt atataaccat gttgtcccta aggtaatcaa
     7501 agccgtattc attatttgtc ccgtatgtta tatcagctct gtaggccttc tgcctgtctt
     7561 ccggttcaag cccgtgaaca ataagcccca cggacattcc gagaaagttg tacagcttcc
     7621 ccatccattg gctctggtat gttgccaggt aatcgttaac ggtaacaaga tgtgtacctc
     7681 tgcctgtaag agcattcaga tacaatggga gcgttgcaac cagcgtcttt ccttcaccgg
     7741 ttttcatctc tgcaatcctg ccctgatgca gtactattcc gcccatcatc tgcacatcaa
     7801 aatgccgcat tcccagaacc ctcacggaag cttcccttac caccgcatag gcttccacca
     7861 gcaaatcatc cagggtctct ccgtcggcaa gccttttctt gaattcatca gttttgctct
     7921 gcagctgctc gttgctgagc ttctgcatgg caggctccaa tttgtttatc tcctggaccg
     7981 tcttcattaa tttttttatc tctctatcat tgggattgcc aaagacttta tctaaaaaca
     8041 ttcagttatc tctcctcata attatttagt tccctgtctg acatatttat aaggataatc
     8101 aaaataatgc attcttccat atgctatttt tcataattat gttatttaaa atacatatta
     8161 gtattgtaaa cgatacacag gcttgaatat tttccgcatc cggatacttt ctcaggctct
     8221 cattattctg aacggagctt tgaatatgcg tccggcttag cgaaagcctt gcattctgtg
     8281 caacgaacct gctcaccgca aatgtattga tatcagaatc aaacacttca gatgatcctg
     8341 aaaacactgt aaaaaccata aaaataaagg ctgcatattt ttttatatta ttatagctat
     8401 tgtgcggcat ttagattcac ctgccctgat tatcacggat tccaatatat ctttaatatt
     8461 ttatcataat ttcatgctat agtctgtaaa attcgcaaaa aacaaaaaaa gacatgggga
     8521 cgtttcggga cacggggggg acatggggac gtttctcctg tcccgctgaa agcgggacag
     8581 gagaaacgtc cccatgtccc ccccgtgtcc ccccatgccc ctgtgtcaca tcactttccg
     8641 gcctttttta ccataatttc ctgaatatac atgaatatac ccagagccat gaatacatcc
     8701 cctatgctga aaattttcgg cctgggatac ggtttcgcca gtaccagaat atctcccaga
     8761 tacttgagtg cagtattgcc attcattaat gtgtgggtaa ttatttcccc gtctcttatg
     8821 gctatcatat tatcaatcaa acctatatcc gccattgcct ccgccgaaac cggcatctgc
     8881 cctccgttag ccattatcac aagaaagtta agaaataccc cgataaaaac tattcggaag
     8941 gataatgtcc gacgattgaa ataaatgcca atgaaaagca gaagatatga caggaaatgg
     9001 acgattagaa tattgtcctt gaaaaaccct atattccttg aagctgcata aactgccgca
     9061 aactcaacca tgaatcccaa aacaaaaaaa taccattttt caaggctggc atcctttata
     9121 tttgagaagc ttcctcctct catctttccg actgcgaggg aagtgcctac actttcaacc
     9181 agcaaccttt ttcatctcct tatcatcctt tatcattctg ataaaagcat ctgcaagctt
     9241 gctttcgaac tggatgtcct tccctttctc aataatatca agggcttcct caactgtcat
     9301 tgcatttcgg tatggccgct ctgaagtcaa tgcatcaaat acatcagcaa tgctgagtat
     9361 ttgtgcttca agtattatat ccttatactt cctgccttcg ggataccctg tcccgtcata
     9421 tctttcatga tggctgagta taatttcgga agcatcctta agaaaatcaa tatccttaat
     9481 aattttgacc ccgatctccg gatgctgctt aatcttgtca aattcttccg tcgttagcct
     9541 tccgggcttg ttaagtatgc tttcatcaat tcctattttg ccaatatcat gaagtatagc
     9601 cgctatctta aggttctcaa cttttttagg aggcagcccc agcctttcag ccagcttgca
     9661 cgcgtattcc ccaactctcc gggaatggcc gtttgtatag gggtctttcg cctctatcgc
     9721 ctgtgaaagg gattttaccg tatctatata tatactcctc atatcaatat acattttgaa
     9781 ggaatacctg gcaaacaaca atggaatgaa gaatattaaa atacctgcca ccttataatt
     9841 tgcataagcc atcgccatta tgacagcaaa gggcgccatt gccaaataat ccttcaccac
     9901 ccaggccata ttattaatga agttggaaaa aaatttctct ccggttataa gatgcataag
     9961 aattgacatt attacggcat taaccagtat atatattacc atcgccgtta taactgcaat
    10021 taatgcagct attgatgagt ttaaatctat tgtgccggga accgccccca aggcttcata
    10081 gcaaagccct gccaatcccg cggaaatagt agcatttgcc ccgttaaaca atgttttttc
    10141 tattggaaaa ttaaatggat ggtatgtttt cccttcatgc cttacaactc taagcataac
    10201 ccctatgctg gttatccagg cagcttcagg cacaccaaag accactatgg cagcaaggcc
    10261 gattataaac ccgacagaca aagccctttg cccctgggta acaataacca ttgattcggc
    10321 aattacagat aatagggtaa aaataaccag acccactgca tcaacctcgt gggcctgatt
    10381 taatgtgaat acaaaaaata ttgttcctaa tagtactatt gctgctacgt atgctttaag
    10441 cttgataggc atctattcac ctcataaaat ggccggcata tgctatgcta ctcctaaaac
    10501 caggtctggt gtgcccctcc ggctaatacc aatgtgaaaa tagccataag aataccaagt
    10561 atctttttca ttgtttgccc tccttttatc aaggctctgc ctcaccgcta gtcaaggcag
    10621 gcaaactctc cgcttaaata acttgccggc catatttgac cctttgttta tgctactgtg
    10681 ttcacttttc tatttactgc tgccagtaca gctttaactg cagattcatt ctcgtcaaga
    10741 ctgattatac tgcttcctga atagtattca tcaatattgt ccttagaaaa acccactaaa
    10801 gacacaaata cctccttatc tgaaacctta aagttttcca gcccctccaa atacagcact
    10861 ccgcttattc caatacaagc ctccagggcc gccagtgttg cttcagcaac tactttggct
    10921 ctgttggacc tggatttgac tttgctgctc cttccttgat aaactttgtc atcgtaggaa
    10981 agcgtcactt cagcttctat cgcattgttt acgttcttaa cctgaatacc ttctatcctg
    11041 actcttgtct ccttgaactc agcctcattg atctgggcaa tgctgatttt tttataatcc
    11101 acagatatat tgaattttgc acttattgcc gattgtatat ccctgaccat ctgcttgatg
    11161 ttcctgcctg ccccagccaa aatatggatc tcttcaattt taccttcctt gtcacatatg
    11221 acgctgcagt ttattatatc gggaagcttg catataagtg attggatatc attatacaga
    11281 atggccttat tattgctcaa agggaactcc tcctcaagtt atatatgccg cggaaaacat
    11341 tagacatgcg atataatagt ttctagaaat acttccttaa tccttcttct cttatataaa
    11401 ttagttaaat tgcactatcg aatgggcaat ttgcactata catataataa ctgctacaga
    11461 ataaatacca tatctccgcc cattagctcc taaaataaaa acagccgatt tgctcggctg
    11521 ttttatcaga attccggttc aatcaaaccg tagttcccgt cctttctctt atatacaaca
    11581 ttgacttctt ccgtatctgc attggaaaac acaaagaagc tgtgtcccag cagctccatc
    11641 tgcagaactg cctcttcaac gggcataggc ttcatggcaa atctttttgt ccttacaatt
    11701 tttacttcct catcctcgaa ctctgccgga ggggcggccc agttctcgaa tttgatgttg
    11761 ccgtctttaa ttctcttttc cagctttgtc ttttggcgcc tcatctgcct ttccaatttt
    11821 tctacaacgt tatctataga tgtatacata tctccggtag cttcttctcc tctcaggata
    11881 accccattaa aaggaattgt gacctcaatg atgtgcctgt tcttttcgac gctcaaagta
    11941 gcctggactt cagtatcagg gccgaagtat ctttcaaact tggaaagctt cttttctacc
    12001 ctttccttca gagcattggt gataacaata tttttcccgc tgactgtagt acgcataaac
    12061 acatctcctt tcgcaactat tatgctaccg tttatacata ttttattcta caaatgagaa
    12121 aataatcctt ctcatttcca taacttccaa acatttatat tcagcagccg aaaagaaaaa
    12181 atgcgtcgca cgacgccttt tttttcgcta gggaacccta aggttcccga aggcgtcagc
    12241 tccgcagctg agcaccctcc ttcagcggca gggggctcct cccccttgca tcccctatcg
    12301 tataggaaat cataatttcc tatacgatgg caaaaataca gcattttagc agcaaatgca
    12361 agtataattg aaactataaa gttatgtaaa gcgaacaaaa ataataatta aaggaaacct
    12421 gcccccaccc ctcatatttc ttagttagtc acattataca caaggttatc cacatctttt
    12481 ttatgttatc tgtgcataat gttgataagt ctgttgataa cttgaatcta ttaactttct
    12541 attgttgaaa aaatgtgaat tgatttgtgg ctgaaaaatg tcgagcgaac aaagtgtaaa
    12601 atattatttt gacttatctt gtcgttcagt gggttttaaa atccctttgc aggtactttg
    12661 ctgtcgtaaa atgatgtata cggtatggga caagttatgc acaggatagc atactttcct
    12721 gtacaataaa aagccgcctt gataggcggt tttaattact gtctttttgc gtcaaaacac
    12781 tctttgcaat ataccggtct tgcatttgta ggtttgaatg gaacctgggt ttctttgccg
    12841 caatctgcgc atactgtatc atacattacg acttctcttc tctgtccatt tcttccgctg
    12901 gcattctttc ttgccattct gcaatctttg catctggccg gttcgttctg gaaacccttc
    12961 tctgcataga attcctgttc tcctgctgtg aatgtgaatt catttccaca gtctttgcag
    13021 attaacgttt tgtcttcgaa catgtaaaaa tccctctctt ttcttctcca ggttggtgtt
    13081 gggtaaaaag aactttctgg gatcattacg ccgcgaaaaa cgttcttgaa ccgcccactc
    13141 atacctttaa atttatggca atactctagc tgacctggtc tttgacccca cactcgccgt
    13201 caggatgttt cgctactagg actcaacccc tcactacagc acctctcaat aataaactat
    13261 tggacggact tattcctaag ccgcaccatg ctcacagaag cttcttctgc ttacgtggat
    13321 cgttttgcct gcgactggca tcgattttca accacagacc tagatatttc accacattta
    13381 atattatata atgtaattct tgatttaaca agtacttttg aaaacaaata ggtattaaat
    13441 atcagaaaat accgtcattt cttcttgtct acatatattc cgtcattgtt tgtaattggt
    13501 ctttcatatg cctttatgga cttcttcccg gcctttatct gctttatttc tcttttcatt
    13561 tcattcatta tggacacaag cttctccttg ttggaccttt ctatgagcat tatttcctgt
    13621 gccattgaca ttatttcttc aactttaagc tttaaatttt tcaattccgg atattttgta
    13681 aaatcaacct cttccaagct attgaccatt aactgttttt tcagcagctt aaacttttcg
    13741 gaaaaagact tgtcaatttc atcaatgctg tcaattacag actgcttctt acttacaagt
    13801 ccttctattt tatttgctgc attctcttca atatcttttt tttgcgccaa agtaatttcc
    13861 aatatgctat cgaataactg ctttttctta tctgtcaggc tgaggagttc aattattaca
    13921 ctatttgtat tatccataaa taacctccaa aaaaattagg aacgacgagg gcgccgtccc
    13981 ttatttgtat ctcgtatctc caaactagcg attaactaca gcctgttgtt tcgctagttt
    14041 catggcctgt gcccaggtat ccctaagctc ctcggcatac cctattactt cttcaacaat
    14101 agccttatcc ttacttatat ttgcttctac caatctcgtg ttcatataat catagagaac
    14161 cagtaaattg ttggatattt catagctcat atcaagggtc ctcatgaact caaataaaat
    14221 gtcctgagct ttaatgatat tattattagc cttttctatg cttttttcat caatacccat
    14281 aacggcttgt ttaagaaacc taatcaagcc attatacagc ataagtgtca attcttgcgg
    14341 cgttgcagta gttaccatat tctttttata tatatcatgt gcattgttag ccattgccat
    14401 tttgttcttc ctccaggtat tattgactat cgtactgctg tgaaagccat actgactgta
    14461 tgttcatttg agatatggct ttttccatag cagtaaactg tttataataa tagtcttctc
    14521 tagtttgtaa ttttttagtc aaggtcgtta tcatatcgct ttgagttttt atcatgctgt
    14581 acataatatt cttattttcc gtcgtttctc ctaccacacc ggccttttca agcaaagcac
    14641 ccttctgccc gtttttattg gaatatgtcc ttatattatc ctgaatgaca tcatacatcc
    14701 tgttaataat accattctct ttaaaacgag tgcttctttc tgttgaagtc atatctgccg
    14761 agtatgcgat gcttgattct ttagaaaata tttctgctac cttgtccggt gaatctttta
    14821 ttgcagcctt caatttactc tcgttaatta caagcttccc cttgttgttc cagctgcctg
    14881 tttcgatccc tatgctgaat aaaaatgatg ttccggattc gccttcaaca ccggcataca
    14941 aagtgttcct taaatcactt actatcttct gcaacaagct gtctccgcga agggtaccag
    15001 actttgcctt ctcttcccac ctttttatct catcttcagt catatcctct ttctggtcgt
    15061 cggtaagagg agcataatcc cggtctcttt cttccgtaag ctcaccatta atttttgata
    15121 tcagttcatt atacttgtct acaaagctct taatcttatt atataccgta tcaacatcct
    15181 gtttggtaga aactgtcact tctcttgtcc ctgtttcctt aagtgcagta taagttattc
    15241 catctgcagt gaaagtatta ctgcttcttg taatttcagt accatcaatt gtgatctttg
    15301 cgtcttcacc ggcattgttg acactgatat ctgcagtggt aataaagctg cttccacttt
    15361 cgctgatttc aatattgttc cctgcgccgg tctgttttgc agtaaacttg aatttgtcat
    15421 ttatttcgtc atactgcata tttacatttg cagttgaatc ccggtttatt tgattcatca
    15481 tgctatataa agtcgttgtt tcgctaaact caaaggtttt accatttata gcaagcttta
    15541 ttccctctgt gccatccgat aaagctgtaa atgccaatcc tccattgcta tcatccagct
    15601 tggactttaa aactgcaagt gtgtcaccac tgctcagtct gttagaatat attgcatcag
    15661 ctccaaaacc catattggcc aaagcattat ttgtagtacc agcactgact gttattttat
    15721 ttataccgtt tgtttcgctg gagactttga atgtaagctt tcccggattt gttgtatcat
    15781 ctactgttac ttttccactg ccaaaggcat tctctatgga cttgttcaag tcagccagtg
    15841 ttactccgtc agtcccatcc aggtcagata aagttattga cttggtaact ccatcaattg
    15901 tcagcttaaa ggtttctccc gctgtcaatg tatatcctga atcgccctgt acatccttag
    15961 taactccacc tgcactttgt cttacctgtg ctgttgcaag atttcctact ataattgaat
    16021 gtgtgccctc agaagcagca gatgttgcca ccgcactaac cgcacctgca tccgcagcat
    16081 cactagggct gacagtagtt gaaaatgcct tgtagcttgt cgaagacctc atattggttg
    16141 atgaaattac atcaaaatag ctgctctgaa agcttctcat aagactggta atacttctgt
    16201 agtcatccct tttccattcc aataattgct tgtcctgctt gactttatcc atcttcgatc
    16261 tttcaacctt cataagctgt tcaaccatat cgtctacatc aagtcccgat gcaattcctg
    16321 agaaccttaa tgttgaaaca ctattaatat tggaagccat gttgcctacc tcctctcgtc
    16381 cacaataata ccggccattt cccataattt agccatcaaa ttaagtattt tttccggtgg
    16441 aatttctcta ataacttctt tagtctcttt gtccactact tttaccatta tcctatttgt
    16501 cttctcatgt acagagattt caaagcttgt aaatgaacct tccaatgctt tgtttgctgt
    16561 ttcaaccgct cttataaact gatactcgtt tatttctgac ttatctttag gttttgcgga
    16621 aacttcctgt tcaccgctct ttactgcatt atttctatta cttatttcag catgattccc
    16681 tttaactgca ttgtttctca tagtatctga gcttacagca gggcttatag aatttcctgc
    16741 tattttcata ttacttccct ccttggatat atttcccgca tccttacaat atgttatata
    16801 tagtatatat cgataaaaaa cacgaatttc tttagctttt ttataactta atagtattat
    16861 tttctagcca ggcttcagtt ttttctattc cttcctgaag tccggtcctg gggctccaat
    16921 tcaaaagctt ctctgccttt tcataattgc agagcagtct ttgtatttca ctttggggat
    16981 gtatatgtgt aacatgcctc acctgctcat aattacccga aatcatatat gccaaagtat
    17041 ttatgctcaa atcttttcct gatcctgcat ttataatttc cccatttgat ttttccgagc
    17101 agcctgccat taccagaaac tccgcgcaat cctccacgta aagcaagtcc cttgtctgct
    17161 tcccgtcgcc gtatatattc aaagcttgac ccttaagctt tctttcaata aatattgata
    17221 caactccccc ttctccgccg gacttctgaa agggtccata tgtgttaaag ggtctcacta
    17281 ccactaccgg aagcttataa gcatgccagt aggacagtgc aaggctctca ccggctatct
    17341 tgcttccggc atatggggat gctggtttta caggatgaag ctcgtttatg ccctgatcat
    17401 tttccgccat gtcatatacc atgcaggtgc tcatgaaaac cattttaaca ttatgtttcc
    17461 tgcattgctc caatacatta aaggtccctg caacgtcatt ctcaaaggtt gtccggggat
    17521 cgtcgatgct gtcctggaca tttatgcttg cagcaaggtg aaagcatata tcaaagttgt
    17581 tttcaaacag tctttccaat atgctgttat ccttgatatc accttttaaa aatcccatca
    17641 gggctttatg tccttcaaat tcctttatgt tatccaatga gccatttgac aggtcatcca
    17701 gtatccaaac cttgtggccg ccttcaagca gccttttcac tgtccagcgt cctatgaagc
    17761 ctgctccgcc tgtaacaagc atgttcaatg tatatcaact ccttaatata tccctaagat
    17821 tgtcagctat ataatatata tcttcaattt ccattgttgg atataatggc agtgttatta
    17881 ttccttcata aagggcttct gcttcgggac aaagcccttt ttcatatccc atgtttttgt
    17941 aatagggatg ataatatact ggaatataat gtacttgtac gcctatattc ctatccctta
    18001 acttgtcaaa aacttctctt cttccaatgt caagcttgct caggtttagc tttattatat
    18061 ataaatgcca cgcagagctt gcatattcat tttggtatgg aattgttaat ccatcaatag
    18121 taccaagcaa tttattgtat agagctgcat agcttcttct tttctccaga aatgaatcaa
    18181 gcttcttcat ttggctaact ccaagggctg cttgtatatc agtcattctg taattaaagc
    18241 ctaagtactg ttgctcacaa aaccaaagtc catcatagtc agtcattttt tccgcatttt
    18301 ttgttatacc gtgagttcta aacaaagtta gcttttcata taaggatgca tcaccggttg
    18361 taatagctcc gccctcacct gtagtaatat gcttgacagg gtggaagctg aaggttgtca
    18421 tggttcctat agtaccgatt tttctgcctt tgtattccgc tcccatggca tgagcaccgt
    18481 cttctatgac agttaatccg tgattatccg cgattcttat aatctcgtcc atttcgcagg
    18541 gctgcccggt aaagtgcacc ggaattattg cctttgtcct atgggtgatt ttcttttcaa
    18601 tatcccaagg gtcaatatta taggtcatgg gatcgatgtc tgcaaagacc ggtttccctc
    18661 ccatatacaa aatgcagtta gctgtggctg caaatgtcat gggtgaagtg attacttcat
    18721 ctccgggtcc gatgcctgcg gcaaagcaag cggcatgaag agcagcggtt ccgttggata
    18781 atactgcagc atattttgcc cctgtatact ccgttatttt attttcaagt tcgtcaacct
    18841 tgggccctgt ggttaaaaaa tcgctcctga gaacttcaac caccgcctgt atgtcatctt
    18901 catcaacata ctgtctcccg tagggtatga acttcctcat acagtacctc ccttttcctt
    18961 ctcacagatg attttccata tctgctccga tacccttata cttccgtaac catccactaa
    19021 ttcccgggct gtatccgaca ttcttgccct tgtattataa ttcaagcttt ccagttgctg
    19081 aacaatttct ccggcacctg ccgcatattt taatgcacct ttaatatcca tgctgcgtga
    19141 cgtcataatc tggttaggtg ccgcagcgac tccgataata ggtgtcccac aggcacaaag
    19201 ctcatagacg gtacttccgc atgcggaaat ggcagcatca catttcagca taagctctga
    19261 catcttggga ttatagtgga gaatgatatt tcctcccgct attctctcaa tactatccct
    19321 attagtaaaa gatggtccca ccgctatatg aaatgtgatt tcaggaaaaa ccttactgag
    19381 ctttaatgca atagtttccg acaggttccc gggatccgcc cctcccaatg taatcaatac
    19441 gtcttctata tgcattcttg tttttctctt gggcaggttc ctgaattcat ctctcagaag
    19501 caaataccgt gtacccaaaa ataacttggt gccaataccg gctttgtatt ccatatcctc
    19561 ggcataaata ttctgattga taataaaatc ggcataaatc cggactttat ttatatcatc
    19621 catatatccg gttatgccaa aaatatcttt agtcctatca aaatagcctt catctatatc
    19681 ataactgtct gtaatcaggc aatccccgcc tattgcagcc aatccgtcaa tgaccgtctc
    19741 tccgctgaac ctccatactg aatagccgca agcttcaatt tgccttacgc ccggttcaaa
    19801 ctccttgtta tccctgcagg caaaaaccac ctctgcaaag gctttcagct tatccgccaa
    19861 aaccagcata cgcattacgt gacccatgcc gatcccggga cctccctctg ccctgattac
    19921 tatcttcata agcccatctc ctgaatttct atactatacc agcatatccc acagcaatac
    19981 atcatcttcg aatatatcaa cctttgcagt tctgccaatt accatatcaa aatatttagg
    20041 ctttatacca tagcccggtc tttttgcggt tatcatactt tctgttattt tcgtaccttt
    20101 tggaatatca gttatggcta tcagacttct tcttcccttt tcgtagttct ccagttcttc
    20161 ccgcgaaacc tttttaactc cgtcccccat agccttttcc acatccctga tttgccgcac
    20221 catcagcttc agttcctccg gttcaattga aaaaccatga tcgggacctt cctgctctct
    20281 gcttaaagta aaatgctttt ctattatgca ggcacccaaa gccactgctg ccacaggaat
    20341 atgccagcct aatgtgtgat ctgaaaagcc tgtgggaagc cggaaggtgt ttcgaagtgt
    20401 ttccattgct ttcagattaa caatgtgtgc cggtgccgga taacaggtat tgcagtgcag
    20461 taaagcaata tctccatttc ctgtacttct cgcagcttct atagcctctt caatttctcc
    20521 aaggctcgcc atacctgttg acaaaaccac aggctttcct ttaccggcta tatattttat
    20581 cagttttaaa tcaactattt caagagatgc tactttgaaa agactgacac ctgtattgtc
    20641 aagcaaatct accgcatcat aatcaaaggg cgaggatagg aagtctattc cgaagccttt
    20701 agcatgttcg gacagtatcg gtatccattc cctgggatgc tgaacctttt taatcaaatc
    20761 ataaggcttc atggaatccc ttgaaaactg aggagttttc ttagaatata aatcctctgc
    20821 cctataagtc tgaaacttta ctgcatctgc gccggcttca gctgctttcc ttatcagctc
    20881 tttagctgtt tccaaatccc cattatgatt ggcagctatg tccgtcatta tatagcaagg
    20941 attatcttca cctattcttc ttccgttttt cagaacaatt tcattcatat ccggactcct
    21001 taaacatgct tttgcacaac ttcactgttt atggctgcaa tatcagggtt attgtctaaa
    21061 aattttacca catccccggc agatacatat atgtctccaa aatgctcata tatcttctca
    21121 accaatgtga aatcctctaa agtatccaca cacagcctat agttcttttt gaacaaactt
    21181 tctcctcgga caagtcctgt acggaacagc tccggatgaa ggtacatata ggcggtaacg
    21241 tgttccttat acctgtactc gtccgccaaa tcaaaggcct taaacagagc ttcccttgaa
    21301 aacacttccg tatcaaagcc tctgacaaat gtatccggta catccaatct gacataatca
    21361 taatcgtaca tcttataatg ggatattaca ttgtcggcta tatcaggatc aataaggggg
    21421 caatcccccg tcacccttac gacaatatct ccgccatatt ccagaacaca gtccctgtat
    21481 ctcttaagga cattctcttc atctcccctg aaaacctcaa agccttctcc tttgactgtt
    21541 tcatacaaag gatcgtctaa ctgcctgtcg gaggttgcca gtatcatcct gtcaatttcc
    21601 cttgattttt ccagccgctt cagattcaac aatatcaggg ggatacccag aaccttcctg
    21661 agcacctttc ccggaagcct ttccgatccc attcgggctt gtactataca tgtaaccatc
    21721 ctatcaccgc attcttaatt attccttaat tgcatacata taaattgatc ttcccatccc
    21781 aatatttgca agcccttcat aaaatctgtc caataattgc tttcgccctg tacttttcag
    21841 tgaattctca aaattatata taaatggccc cacttgcttt ttatattctt cctccgtgta
    21901 gtaattcatg cctcccataa gcagtatttc caatgggaaa tccgttgtcc tgtacaattc
    21961 tctgaagcct gtccttgaaa gcaggccgct aagcgaatta atgttaaagt aatttatatg
    22021 gtcaggcagc actatccatc tatatgcctc gctatggtat tctttgaatg ccatttgccc
    22081 ctcactgaaa tcattaggta cacaaatcct gattatacct cccggcacca gcattctata
    22141 tgctttctca aggaatgctg cagggttatg aatatgttcc aggacaaatt gtacatttat
    22201 aaatgaagaa ccttttaaaa tttccggttt gatattttcc atatagtcat ttattacatt
    22261 gaggttatag ctattaagat attctgcagc cttaatattg ggttctacta cgcagacctc
    22321 ccactcctta ttctgaaaat ataaggccag caaatcattt ccgctgccga tatcaaccat
    22381 tacgttttca tatgcccttt tatattcgcc tactttgtca tatattgcac catatatttt
    22441 attgttgtca acttcttcag atttccttag gatgaagtcc tccgtcacat ttttataata
    22501 aaagggtttt atctctgaat aatacttttc atcatagaac tccttcatac tttctttttc
    22561 aggtacgggg tatagatgct taaagccgca atccatgcac tcaataattt tgtattcttt
    22621 gtgttcccaa tgtttttcat atctatgttc cataataatc ctccggattt tatttcaacg
    22681 tatctatcac cgcttttagc tcatacatat ttgttatctc tctatccgct tcataatcac
    22741 tgcccaatct taatcccatg tgatccccgg ccggtcttat aatcctgacc gtatatactc
    22801 ccaattgcct tgccccgcag aaatccttcc ggggattgtc tcctacatat atcatactgt
    22861 cgagcgcaac tcctaagttt tcagccattt ttaaatatgg tttgatgtcc ggcttgcaga
    22921 aaccctcccc gtgttcatca gttacaataa tagaatcgaa atatttctca ataccgagca
    22981 gccgtatctt gttccgctgc acataatata gcccgtctgt aatcaagcct gtcttgtact
    23041 tcccttggca gtatttcaaa aaatactccg catcttcata tagggagatt ggtggaactg
    23101 catttctgta tatctttata agattattta tatcttcatc caagttgtat aaatcacaga
    23161 tatcattaaa tattctgccc ctgccctttt cttgaagcag ctttaacatg gcatgatata
    23221 gctcatgact aaagatgcaa tgcttttttg aaaggtattg cgcaacttcc ataaaggcac
    23281 cattcacgaa atctgcttca ttataaaggg tgtcatccaa atcaaaaact aatgccttca
    23341 tataaaaacc gcctcatcat atctgagcat tgtaagctct ttaaagtttc caagcagtat
    23401 ttccggcttg ttgccttcaa tcaagtcaat gaatatcttt ccataatcaa ttccggcttc
    23461 cattgtgagc ggaacaccgc ccccaaccct gacattgact tcagtgaatt taatatcccc
    23521 ttcaaccgtt ctgaaacact gaactgtagt cgggcctgcg ctgccaaggc actccagtaa
    23581 atacctggtc ttttctatta tttccatatc tttaaccgtt cgtgttttgg taacctctcc
    23641 tgcccttacc tccagccgct ctcttggtac aacggatata actttgcctc ctaaatccga
    23701 taagcagtcc atggtatatt caacccccga cagtttttcc tgcactatgg gttcaggtat
    23761 acgttcatag taaaaatcaa attcctccat atcatttaat tcatatgaat tgaagctgcc
    23821 catacccctt ctgggtttta tgaacatcgg aaattcaata ttgcactctg ccttgttctt
    23881 gaagcttata ggcgtactga tattgcttcc gataaaaaac tgatatgtct cccatttgtc
    23941 gctgcatata tccaatacac gtttatcgga caacaacaaa agggttcctt cattcaaaag
    24001 ctcttccctg atagaatcca ataagtaaaa ttccttttca taaagaggca gtaaaatgtc
    24061 tattccatat ttcctgcaaa tattgagtac ttccccaata tatccgggat tatcacaagg
    24121 tgataccgga caaaaacggt caacatgaaa tgatgcaggc gcaagcatcg agcagtctgc
    24181 acctatcacg gtaaattgtc tttttaagta ttttattagt tgtacacgct tgcctacggc
    24241 ggtaataagc agcttcatag caccacctcg ccttactgtt ttggttcaaa gctgtccaat
    24301 atacttttga gcaaaggaag cctcttgtcc ttatctgaca gtattatacc ttcaacttta
    24361 tcagtaggcc agtctatggc tatatcagga tcattccata taatgccttt ctcatattcg
    24421 ggataataaa aatcagtaca cttataagca aattccgccg tatccgacaa taccaggaaa
    24481 ccatgggcga agccttcggg aatataaagc tgtttcctgt tttccccgct taatacagta
    24541 ccataacaac ttccaaaggt gtctgagccg gctctcagat ctactgctgc atcaaatact
    24601 tcgccgtgca atactctcac cagcttgccc tgtggacgtt ccttctgcag atgaagccct
    24661 ctcaatacac ctttcccgga actggactga ttgtcctgta caaatatcat atccagccct
    24721 gcttccttaa aatcattgta attatacgtt tccatgaaat atcccctgcc gtcttcaaat
    24781 acagcaggtt caataatata aagccctcta atatgagttt tgctaaatat aaaccgtccc
    24841 aattatgata cctcactttt caatacatct caggcatttt catacattga gctgtagtac
    24901 ttgcgatatt ctcctgaagt aatattgctc atccattcga tgttatccaa ataccattct
    24961 attgtcttta caatcccatc ctcaaagcct gtttcaggag cccatcccaa ttcttcccct
    25021 atttttgttg catcaattcc ataacgtctg tcatgtccct tcctgtcctc tacatatttg
    25081 atcagtcctt catcgatttc cttatcgaca ttatcatgca aataatttat tatatactta
    25141 actatatgta tgtttgtcct ttcgttgctg cctccaatgt tatacacctc tcccggttcc
    25201 ccggatttta tcacgttatc tattgccttg cagtgatcct ccacataaag ccagtctctt
    25261 acattaagcc cgtctccata tacaggcaaa gactttttgt tcagacagtt gttgataatc
    25321 agaggaatga gcttttcagg gaactggtaa ggtccgtaat tattcgagca tctggttatg
    25381 tttatcggca ttccgaaagt atcataataa gcctttacca caaggtcggc gccggctttg
    25441 ctggatgagt acgggctatg gggattcaga ggtgtatttt ccgtgaaata tcccgtatcc
    25501 cccagagagc catatacttc atcagtagac acctgaagga actttttccc tcttctgaag
    25561 ccctgtccct ctgaccaatc tgcttttgca ccattaagta agttgactgt tcccagcaca
    25621 ttagtcctta taaatatctc agggtctttt atacttctgt ccacatggga ttccgcagca
    25681 aagttcacaa catagtcaat atcatgttta tcaaacagct cacttaactt ttccttatcg
    25741 cagatatctc cttgtacaaa attatacctt gaatcctttt caatactttt cagattttcc
    25801 ggatttccgg cataagtcaa cttgtcataa ttgatgatgt aaatgtcact atacttattc
    25861 aacatatatt gtatgaaatt ggaacctata aagccggctc cacctgtaat caaatatgtt
    25921 ttcatatcag cagccccttt ataaaatgct caatggcagc ctcccagctc cgcatgtcat
    25981 ctcccaccgt cagtctcagc atcatattat ccattactga atatgcaggg cgctctgccg
    26041 ctctgtctag ttggttcgtc ttaataggct tcacagggca atcaattcct gcaatctcca
    26101 atatcttgat tgcaaactcg taccagctgc attctccatt gccgctgcag tggtaaacgc
    26161 catactcgtc agtcactgct atttttaaaa tgtggtatgc taaatcctct gcatttgtag
    26221 ggcatccctt ctggtcatcc acaacttcga gatactgctt ctctttagct tcactgagga
    26281 tagtccggac gaagttccga ccatttcttc catataacca tgaagtacga ataataaaat
    26341 attttttgca aaactctctt acatactcct cgcctaagag cttagtcttg ccataaatac
    26401 tttttggtga agttttgtca tattccctgt atggagtact cccgccaccg ggaaaaacat
    26461 aatcggtaga aatatgtata agctttgaat ttgtctcttg ggcagctata gcaacattcc
    26521 ttgctccaag tgcgtttact ttgaacgcag cttccgcttc tttctcacag ccgtcaacat
    26581 tggtatacgc tgctgtattt ataataacat caggcctgaa cctgcccact atattcctga
    26641 ccgcaggcag gtctgcaata tccagctcct tagcaccaag tgccataata tcaggattat
    26701 tataagacct atcaattgca cctaaagagc agttcatctc cttcgctatc ctgaggagtt
    26761 cacttcccaa ctgccctgct gcgccagtaa tcagcatttt cataccattt ctcccctatg
    26821 tcatcattct tttgttatgg cggcttccct caatatctca taatccctgc aaagtattcc
    26881 gctcaaatcc ttatccttcg ccagctcatt ggcataagca aaggagctga aggtaccggc
    26941 atccgtccac cagccctcaa gcacatcaaa ggtaagggag ccgtccttta tataccagtt
    27001 gttcacgtct gtaatctcca gctctcccct gttagaaggc ttcaaggact taattacgtt
    27061 gaatactcta ttgtcgtaca tatatatccc ggtaacacag caatcactct ttggcttttc
    27121 gggtttttct tcaattccga cgatattccc gcccttcagt tctgccactc cataccgctg
    27181 agggtcgggc actttcttaa tcaatatttt tgcacccttc tcctgctgca taaagttttc
    27241 cacatattcc gaaatatcat cctcaaatat gttgtctccc agtattacaa gacatctgtc
    27301 atcgtgcacg aattcctcac acaggccgag ggcctgagct atccctcctg gctgatcctg
    27361 tatcctgtat gtgaatttca ctccataatc atgtccgctt cccaatagat ttactaccgc
    27421 acccatatgc tccttacctg taatgaccat tatatcgcat attcctgcct gcttgatctt
    27481 tgcaatcaga tggtcaatca taggatgctt gcccaccggc agcagatgct tgttggttac
    27541 tttagtcagg gggtaaagac gagagcctgt cccacctgca aggataatgc ctttcatata
    27601 atacctccct tgattggata tgattataat tatttatgga gacctttgcc ctttagtatc
    27661 aaagcttcat ataagcgcat gtaattatca agcattgcat taagagtaaa gctgctgctg
    27721 taaaaatcat aggctttttc tgctataaca gcactttcgt catcggtaag cgataaaacc
    27781 tgcttaatta cacctgccag tgaagccggg ttattggact tgaacaagaa gcctgtctcc
    27841 ccgtgcttta tcaattctgt aatactcggt atatcgctgc ctatgaatag taccttttct
    27901 ctaaagcact caacagtcgt aattggttgt ccttcactta aagaaggcag gacaacgcaa
    27961 taaaagtatt tataaaattg atatgagttt tccttatatc cccaaaacct aactctatct
    28021 gctataccat attcattagc tttctgcatc agttcgtgta ccaactcacc ctcgcctacg
    28081 aacacgcaga ttgcatcctc tgccttcact acctctttga atgcttcaag aagaagtccc
    28141 tgattctttc tcttgcaaat tgttcctatg cagcctatgg tttttactcc ctcatgcttt
    28201 agtttaatca tttccctgac atcatcatct actttatcag attcactaat atctgtgcat
    28261 gcaattccgt tatatatcaa acctattttc tctttattaa tacctttatc acacattaag
    28321 tcaaaggagc tttgtgatga ggcaattatt gcatcaagtc cgttcattat gattttgtcc
    28381 gtttcctcct gttccggggt cttatttgtt ccccagccct gcatggagtg tattacaggt
    28441 atatacttgt gtgatgcttg tctggatata agacctacca atgcaggtat tgccgcatgt
    28501 gcatgtatta aatcgatatc actgttaatt attatttctt ttactttctc aacaaccttt
    28561 aaattaagat acaagtctct tttaaaggtt gagtctattt catataatat aacatctgca
    28621 ttattaagcc tgtttatata ttccgcataa ttgcaatagt cgctttcctg ggtcttgctt
    28681 gttacgacat atacattatg tcccagcttt ttctgttcaa tcgcaagatc acatatgatt
    28741 ctgcctgcac caccctgcaa aaaagttgtt atgtgcaata tgttcattat ttatacctca
    28801 taagccaatc ccaggttatt tctatgccct tctccaatgg aatctgcgga aaccacatca
    28861 atttatctgc agcctttgaa atgtcaagta catttaccgg aaggtcaatt tcccttgatt
    28921 ccttatttac tacctttgct tccttgctgg taactctgct tataatatct accaaatcgt
    28981 tcaaggtcac acctttaccg cttccaatat tcagaacctt gatatctgta tgcatctcag
    29041 aagcgaggta cagagcctgt actacatcac ttataaatat atagtcacgg catacactgc
    29101 catcacccca aatttctatt tcttctcccc tcagtatttt acccagaaaa acaccgaccg
    29161 ctccttgatt tgaaaagggg ttttgaaacg gaccataagg gttggatatt ctaaacactt
    29221 gataatcaag cccatataaa tgattaaaca tatgcagata tttctctata ctaagctttg
    29281 ttatcccata agagcaaatc ggattggtag gatgatcttc actaatcgga atagtctctg
    29341 ctaatccata tattgtgccg ccagaggaag gaaatattat cttccttact ttatgcttac
    29401 agcaaagctc taataattta atagtatcca caacattcga ctgtatgtca aatgaaggat
    29461 tatccattga agtctttgga atggtcgtac taatcaaatg ataaacaata tccattcctt
    29521 caaggacatt ctcatattga gcaatctcag aaaaactgcc gattgtatag tcgcaggaat
    29581 ccaattttct ttccggttta aaattcttct caaacactgt aaccttatgg ccttcagaaa
    29641 cgagcttcag gaccaggtta tgccctatga aaccctgtcc gccaataacc agtatattca
    29701 tgagccctcc tactgtataa catcttctat cattagaatc ctaataatac atcggaattt
    29761 ttacaacatt tctttaacag actgtatcgc tgtgcgcccc catattaaaa aagaaagctg
    29821 ttaaagcatt tctgcattca gcaactcctt gtattgataa tttacataga cagaagctta
    29881 tcgtatatct gctcgtattt tgaaaccatg gcctttacgc agaagcagtc ctgagctctt
    29941 ctccgcagct cgctgccttt tgctctataa gcctcctgat tatcaagata gtaaattgtc
    30001 ctttcaacca attcttccag actgtttcca agagtcactt ctccgtcaag tatttccggc
    30061 aaagcgccaa tgttgaatcc cgtaactacc atctctttat tcatggcaaa gggtgttacc
    30121 tgtccgaagc tttccttcca tacaggtgct acgaatatgg aaaacttgtc atatatggcc
    30181 ggaagttctt tataagggac ataccccgca aagacaaaat tctctcttac attttcctta
    30241 tatacctgcc ttacatatga attgaaaaag gtaccatacc caactatgta tataagcagc
    30301 tccggctttc gtttagctaa ctcaataaac acttttatag agtcttccct gagcttatcc
    30361 tgctccagcc tgtataccat accaaccgca tacggccttt tattgtcttt actgggttca
    30421 aaatgggaaa ggtcaatgcc gggatgtatt acagatgaat tctcaggaaa atccccaaac
    30481 atccttctgg catattcact tacatacaca tattcgtcta ctttattatg cgcatatgca
    30541 gcaataggcg tgttgatatt ttccacaacc ttgcagtcat acgtattcaa ggaatcaaaa
    30601 atatgctcgt accaggtatc tccccagtaa tgaatatgca gaaggtctgc atttttactt
    30661 tttaagaact cagatacttc ttcaccggat ttggctccgg aaaagtcatg tatagtcatg
    30721 cctttgtgca taccctcctt aggtaatgct gcagtgatta tctccatgtc atatttatgg
    30781 cctaaatgct ctataagatc aacaataagc tgggttgaac cgccaacaaa ggtgttcggt
    30841 atagcatgaa taatcttagg cctttcttta agaatatcat ttttaatact cacatcatat
    30901 aacacaggtt tgtctaaacc ggcagcacga agctgttttg acagttcttc agccatttta
    30961 ttaaaatcca gcttctgctt ccattttctc tgttgctcat cccaatatgc agagcggctt
    31021 acctcatata tatattgtat tttgcaatgc attgatgtta tatattctct aaggctttta
    31081 ttgcttctct cctttgcact caacttaaaa acttcgtatt tttctatccc catattactg
    31141 agctgctttg atatttcatc ataatacatg cttgttataa tgatcttgtc aaagtccagc
    31201 atcttcagat tgtcgggaga aaccacctca acacctcgga agcttgttcc ccacttgctt
    31261 ttatcattat cacagaaata agcaatcttg tattcttttg tatatttttt catggctgct
    31321 tccccggatt ttgaagcacc aaacagtatt atactctttt tcatctgtaa tgctcctcgt
    31381 tagctttcaa ctaatgccct tcgtttcgtt actctcgata tgtcagcgaa ttaattcact
    31441 aaaatagcct atcgttctta tcaatccgtc ttctactgca atgctgggct tccatccgag
    31501 aaccgattct gctaagctta tatccggctt tctctttttg gggtcatctt cgggtaattc
    31561 cttaaaaatt agctttgaag acgagcctgt cagctttatt accttttcag ccagctccag
    31621 catattgtac tcctccggat ttcccaagtt aatagggccg ttgatatcct ttgatgtgtt
    31681 catcattgat atcaatcctt caagcaggtc atcaacatag caaaaggacc tggtctgact
    31741 tccatcacca tatatggaga tattctcgcc tttcagtgct tgcactatga aattgctgac
    31801 cactctgccg tcttccggat gcattttggg tccataggta ttaaatattc ttactacttt
    31861 tatatctaca ttatactgac gcatataatc aaaaaacagc gtttcggcac atcttttgcc
    31921 ttcgtcataa caggctctta ttcctatagg atttacattg cctctatagc cttcatcctg
    31981 tatatcaatc attgggtctc cgtagacctc actggttgaa gcctgcaata tctttgcatc
    32041 atactttttt gccagctcca gcatattcat cgcacctata acattcgttt tcaaagtttt
    32101 tactttgtca tactggtaat gcaccggcga tgcagggcaa gccagattat agatttcatc
    32161 tgcttctatg tcaataggat tctccacatc acctgtaatc agctcaaagt ccttactatt
    32221 cataagctga gcaatattcc ttttagatcc tgtaaaaaag ttatccaggc atattacatg
    32281 attgccatct ttcagaagtc tttcacacaa atgtgaacct ataaagcctg cgcctccggt
    32341 aactaaaatc ctcttatttg acattggtat ttctccctat acaataatat tcccagccat
    32401 tttcttgcat tgctattgtg tcatattgat ttcttccgtc aaatactatt gactgcttca
    32461 ttctggattt catttctcta aaatccggtt gtctgaactg tctccactct gtaactaata
    32521 tcaaggcatc caccttatct atggcttcca tcatatcata aacatattta atattatatt
    32581 catcttcact cataatacac tttgactgct cgattgcctc cggatcatac gctattatat
    32641 tggctcccat accggtcagt tccttcataa taaccaggga tggcgcctct ctcatatcat
    32701 ctgtctgagg tttaaaagca agtccccata tgccgaagct catgtccttc aaatcacatc
    32761 cgaacctttt tgttaccata ttaaccaagt agtgcttctg tgactcattc acttcttcaa
    32821 cagcttttgc aattttcatt tcgagtccgt ttctgcttcc caagcttatc agttccttaa
    32881 tatccttggg aaagcatgag cctccatagc ctgtacccgc atacaaaaaa gccattccta
    32941 tacggctgtc catacccatg ccaacacgta cattatttac atcagcaccc gtaatatcac
    33001 ataagcgtgc aatttcattc ataaagctta tccttgctgc cagcattgaa tttgccgcat
    33061 atttggttag ttccgcagat ttaatatcca ttattaatat tggatgctga ttcctcacaa
    33121 atggctcata tagctgcttc attaattctg cggttctggg attatctgtt cctattatta
    33181 tcctatccgg tctcataaaa tcttctatag ctgctccctc tttcaagaac tccggattgg
    33241 aaaccacgtc aaaatcaata ttcaattcat tccttaacct taactcttct cttactagtt
    33301 ctttagtctt ttctcctgta ccgacaggta ccgtagattt atttataata atagtatact
    33361 tgttcatatt ttggcctatt gcgccggcaa ccttaagcac atttgtaata tcagatgaac
    33421 cgtcttcacc cggcggagta ccgactgcaa taaagcaata cagcgaagac tctatccctt
    33481 cttttatgtc ggttgtaaac tttagtctgc cgttttttga atttctttca atcatttctt
    33541 tcaaaccagg ctcatatatt ggggaaatac ctttattaag catttcaatt ttagcattgt
    33601 caacatcaac acaccatacc ttgttcccca tctctgcaaa gcaagtgcct gtcaccaatc
    33661 ccacatagcc tgtcccaata acacagatat tcatatatta atcctccgct acacattaaa
    33721 tattggcgtc attcccacag ctgctataat tgtggcacta taatatctga agcttttaca
    33781 aagcctaact tctccagcac aattaaaatc tcatctataa aactgctggc aataacgatt
    33841 ttgtcaaaat gctctctttt tgacaacagg tcttcagggg gtataatctc tctgcccata
    33901 aagtgctttc cccatttatc ggaattagaa tcagaatagt atacaatttc actttcgcat
    33961 tgttctacta tccttgtaaa ctcttcactc agtgatccgg taccatacag tacgtatttt
    34021 ttatctttct caataaaaag gccaagtaaa tccttatata atgtactatt atattggatt
    34081 gtctcacttt ctcccgatag tatgagcttt tcattcaaaa tatccagtat cgtgtctata
    34141 gtattcttaa ttctctcatc attgaactta ttatgccaat tattattacc gtgtactctg
    34201 taaaaaccca aagcttcatc tatagaaaaa attttggagt aatatagtgc attccatata
    34261 atataagcat cggcacatag ccttaatgag acctcaggaa taggaaatac ttttcccaaa
    34321 agagcttttg aaaaggacag ccctgatgtc gggacccagt atcctaaaag ataaccatac
    34381 ttttttaatg cagtgctcca atcaacatcg tttcttaagt tgatatactt ctgttctcca
    34441 tttatcaata gattgtgctg aacaatatca tagttttcat ggcactttac tatcctttca
    34501 agcttttccg gaaaccaata gtcatcgcta tccaacaaag cgattatttc tcctcctgcc
    34561 atgttgtatc cggtattcat agcggaagcc tgccctccgt ttttcttata tacaggagtt
    34621 ataattctct gattctgctt atgatattgc tcaatgatgc tcactgaatt atcagtggaa
    34681 ccatcatcta caattatcaa ttcgaaattc ttatatgtct gcttcaacac tgattctata
    34741 gcttcagata taaattccga atagttataa ttattaataa caacagatac aaaaggattc
    34801 ttagacatat atatcccctc actcgttaat ttttctatat caccgcatag catatggtat
    34861 cacgcacatc ataaccataa tgccttataa atatcttata ttcaggcacc aaagtcttta
    34921 tatataatgg aatctcccag aaatcctgcg gaggcatatg ataggtacat atagccaact
    34981 tgggcttgtt ttcccggata atattctgtg cacctatcaa tgcattcatt tctgcacctt
    35041 cgatatccat tttaataaat gttacatctt catcttttaa tacgtcatcc agcttataaa
    35101 tttcaatatt ctccgaacca ttattgctta tatgcgaacc accaatttct cctggtgtaa
    35161 atttaatgac accgcttttg tcatatactc caccggctat cagtttgata ttgttgtctt
    35221 tatttactga atcaatggtg ccggccacct tatcaagcat attcttatca ggttcaaaaa
    35281 cgaaagcttt attgaagcgg cccttgacct tcgcaataaa ctccattgta gtttgcccgt
    35341 cgtaggctcc tgcatctaca aaggtttcat ggtcacagaa ctccaggatg ctgctatcaa
    35401 agtactgttc atggtcgata atctcattta tcaaattaaa gtcaaaggac atcttgtgtt
    35461 ttatgatatt tatgaatacc tgctttgatt taatatcctc caagatatct agagcttcag
    35521 caattcgttc acaactgcag gaaattgcct cctgactttt cttataatat gataatctgc
    35581 catatatctc acgaggtata aaatatatat cactttcact aaaccctaaa tgaagcaatt
    35641 gcctttcaat ttccgccctg tacatactgg taatcaatat attgaccctt ccataaagcg
    35701 ccaataattg atccggagat attatggccg caccactgat aatgctgcca catttgctta
    35761 cgtcattatc gcaaaaataa tcaaccataa tattattcat cctcaagctg tcctgtgcat
    35821 atagcccaaa ctcagatgct ccaaacagca ccaaactctt atccttatct tccaaaaaac
    35881 gaaggtaact attgttcgag gtaacatgca aatcatcata attaagaaat atactgtcat
    35941 tggtgctgaa attatcaata acccaattat attcatttat gagatttctc aaatcactat
    36001 caatcgcatg aattactttc cagttgctaa aagtaatctc tctatgactt tgaacttcac
    36061 taatcagctt actatcagct gcatacagaa tactggcact gttgatttcc tttacaaagc
    36121 cgatgtactt aaaatcgtct gccaacagat cctcaatgct tagaaaattc actaaataag
    36181 gtatgctaac taattttgac aagtagtaca caaaaagggc cggatataaa tcggttactt
    36241 caattatgtc tatttgcttt ttatcaatta taccttttat aatactcagg ttttcaacga
    36301 aggttttgtg gtctaattca tcaggttcac tattcaacca tctgaaccta taatactcag
    36361 ctcctgcttc agcaatctct gcagcaccat ttcctccatg agaagccaca attaccgaaa
    36421 tgttattttc tatcagccct ttaattcttt ttattagttt ggcgccttcc gcatccattc
    36481 gaatgcattt agtaattatc aaaacacttc ttaccattta taccctccat taagtgcgct
    36541 aataaacata ttcactaaaa taatgaataa taatccttat aaggtaaaca tccgtttgat
    36601 ttcaaatatt tttccgcatc tgctttcccg ggagaagttg ctatgaaagc ataatcaaag
    36661 ctcaattctt ttagctgccc aggtttatag atattgtatc cttcaaactc cccggtcttg
    36721 tatttatcaa taaatccggc aatcttaaca tcttcataaa aaacatccag aacttttttc
    36781 attatcttac caccgttgct tgcaccccat attatgcatt tcttttctac accaccaaga
    36841 attagcttta agatgcctaa ctcaattctc ttttttgctt tccactcttt gtctaatagg
    36901 caaacctcat ttatatagtt gtcaataatt gaccatttgt ctgcttcggg attgttgtcc
    36961 ttgatattat ataatccttc caggtattcc cccaattctc cattcaactg cccgaaagga
    37021 tgagtataca actgctggag aactccacta ttcctatata taattctaat gcccctggga
    37081 atttcgtcac cattatcaaa gtaattgtag ctccattttg ctttgctcag gttatacttg
    37141 tccatctctg atagttgtgc attatattgt ttatttattt catgtattaa ctcatttaca
    37201 ttgggagcat aggttgcaat ggcagtatta ttaaatccgg aatcccaacc ggaaaaatga
    37261 acgaacacca atggtttgtc gttcgcgtaa taatctttac cctcttgtat ggaaatattt
    37321 ctcttgctaa ggttccaata agccaaattg taaccaggtt cctttagtat atatacatta
    37381 aataaagcag gggctaagtc tatccacttt tgatcgacaa acaagccctt atcaacctca
    37441 tcatagcaca acaaatttaa gcggtttttc caccaggtta taaacttaga agattcttct
    37501 ccccttctga ttcccaaaaa acccagatta tataccccgc gtttcagatg gttgcattca
    37561 agaaaaattc cattattgaa ttcttctcct gcctcaggaa aatatgtgtg cggtgtcaat
    37621 acaatttcat ttttctccag tatgctatcc aattctgaaa aactgctgta taccataata
    37681 tcaggatcca aatatacaaa cttatcttca tccttaaaat tattcagaag atactcaaat
    37741 aaatcaccct tcaccgctgt tgaagcttct acaattgtat gcctgaatat gaaattatcg
    37801 aagtctctta ttccaatatc ttttgataat acaacatagt caaaatactc aaacttttca
    37861 gcagcctcct ctattgtttt ttctaccaga catactacaa attcagaatt aggattatgc
    37921 ttcttcagtg attctgctaa aaccatggct tttggcaaat aattggcgca tactgatgta
    37981 caaaaaatca tcttaggttc ctccagtatg aatttatata aattttttct ttaactcagt
    38041 ccttactaaa tcctctggtg tgtttatttc cataattgac tctttaataa tcctgacaac
    38101 aatattataa ccatgctcca atgctcgcag ctgttccaag ccctcgcatt gctccaaggg
    38161 agtgggttca agccttacat atttcaacag aaactctcta gtaaaggcat aaagccccag
    38221 atgctgatat acaggggctt ccagcttggt tctatagtat gggatagctg ctctggaaaa
    38281 atacagtgct ttattgcttg tactgcatat taccttaact acattgggat tggtgtaatc
    38341 aagctcctcg ttaagagggc aggctaaagt taccatatca ggcatttgat tatttaagta
    38401 agggtggatt agttccgtta gtatatcaga ggtaataaaa ggctgatctc cttgaatatt
    38461 cacgattata tccgcttcag gaaacctttg tgcaacttcg gcaactctct cagtgccatt
    38521 tgagtggttg gtatctgtaa gcatagcaac tccttcgaag ctctcaacgc actccttaat
    38581 cctgtcatca tccgtagcga caataacttt attaaaaaac tcacattgct tagttccttc
    38641 atatacacgc tgtatcatag gtttgcttcc tataagcgcc aagggcttcc cagggagtct
    38701 ttgtgagtta taccttgcag gaataactgc cagtattttc attacaatcc gctcctaaca
    38761 atatcatgta gccttatcat acctatagaa acattattat tatccactac cggaagaaca
    38821 gatatctgag atatacggtt ttccataatg ttcattgcct caaatgccaa tatgtcttgt
    38881 gtcaccttaa ttggatcctt tgtcattacc tgttcggcat ataaatcgta aatattattt
    38941 ggtaagttat tctgaacagc tcttctgatg tccccatcgg ttactattcc aataagatgc
    39001 ccgtcttcag ctataacact cacagcccca agcccgccat cacttattgc ctttataatt
    39061 tcatacaaat tggaatcccc atatatcgta ggattacttt ccttaacatg catcaaatcc
    39121 tttactctta atgtcagcct ttttcccagc ctgcccgcag ggtgattcaa tgcgtaattc
    39181 tcaggcccaa tattcttcgc ctccatcaag gttaatgcta aagcatctcc aaaagccgtt
    39241 acagcagcag cactggtcgt aggcaccaaa ttatattggc atgcttcctg cttaaccata
    39301 aggtcaatta ctatatctgc attctcggct aaagttgact tcgcattgct gactaacgct
    39361 attattgaaa catttctatt tttgataaag ggaagtacct caattatttc atgggattca
    39421 ccgctgttgc tgattactat ggcaatatct gaggatgaaa caatgccaat atctccatgc
    39481 atagcatccg ttgcattcaa gtatatcgca ggagtaccga tgctagtaaa ggttgctgct
    39541 atcttgcggg caacaaagcc tgatttccca acccccatca gcaatatctt acctttacaa
    39601 tttaatagca gctctaccgc aatattcacc ttttcatagt caaggttatc cgcaatttgc
    39661 agcagttttt ctccctcttg ctgaattact cttcttatat ttccttcata aagcccatta
    39721 ttcatatcaa tttctccctg catttttctt attcagacca aactcgatag tttttagttt
    39781 atctatgata gcttcagcat ctttcgaaat gcagtatttc tcattaaata tgtaaaaaaa
    39841 cttataaata tcttctttat ttataaggga aatgcctttc ttcaatacca tatccataaa
    39901 gcacaacaac atatccttgt tgtgagcttc gtaagagaat cctaatcctc catagctgca
    39961 attcccgcaa agtatcacgt ctttattttt tatcaatgct tccaacccag cctgtgaatt
    40021 tattgtgatg cataaatccg agtaatctat aagtgaatat gtatcatatt cgttttcact
    40081 gtccacaaca acaccatatt tagtaatttt ctctgccaat tctttatctt tattaatcat
    40141 tctaaaagtg agcttgtcat acttattacc taatacatca accaggtcct tttcttttgg
    40201 atgcggcttc actatcaatt ggaagtcatt attgatacaa taatctacta atactttaat
    40261 tatctctaca ggattcttaa agttccatat gccaaataat accgatgaat ccgaatatac
    40321 ttgacctata taaagaatag tttttctgcc gctcttttgg aggaacttat ttgaaggtgt
    40381 tatatgctcc tccatcttta agtttttgat accgtcgata tacttcatgc aatattcaaa
    40441 tgctgttgaa aaatctaccg aatcataata cctccaataa tagcttctgg ctaaattctt
    40501 atttacagtt atacctgata tgttatccca gattgcttta gtcttgttaa aggtattctc
    40561 caatgccaga ataggcatct cattactcaa tgcaaacctt cgcaaaatag ctgaagtgta
    40621 cagatatcct tgcaaaatca ttacgcttgt gacattaaga gattcagtaa ggttttctat
    40681 tgtatttata taattggcac taatgttata ccactttcta atcgcatcct tatgctcatt
    40741 taaggatatg tcaatatcag ttataaccaa ctgcaaatca acacaaatat tataaataga
    40801 tacctcatac agattaattc ccttgtactc aatctgtctg aagcttttat ccattttctc
    40861 aaaatatata ttgtgtgtat tacggttatc ctcaacaaat actagcatgt atccctcgag
    40921 cagccctttt aaatataatc gatcattatc agcacacagc cactcacatt ccttatttgc
    40981 aagcacaact ttgtgaggat ctgtgcaaga agaagtaatc aattgttctt tgcgaatata
    41041 ctctttaaga tcttcttcca aaccaaagct tttatataca ttaatcaaat catatagcgg
    41101 cctcccgttt tcaaagtgct ctccctcatt taatcccatg ctcttcagct gctgtgatat
    41161 ttcttcgtaa tacatacttg caacaaggat taataaatta ttactgtcct cggtttttag
    41221 caaatccggg ctatgtatat cggagtccat aaataaacga tttagtttat tgatattatt
    41281 atcaacataa tatgacggct tcttcggcat atattcagac aaaagcttcg caaagcttcc
    41341 ggtgccaaat attacaagct ttttagcttg tgcctcatat ataatatttt ttactttatt
    41401 aaataaatta aaataatcaa attccattat atcctccgat ggtgatattt ttatgatatc
    41461 ataacttatt actaatgatt tctttaatcc tggatataaa gtatgccttc ttctgtctta
    41521 cttctttata gctttcctct attttccgta taatgttttc tttagccgag taaatttcat
    41581 ccaaaattag atcaaagtct ctataataca acgcatctga caatattata ttgcattttt
    41641 ccaatccgaa aatccccaaa gctccgatat ttttatgctc atagtaatca cctaatgcca
    41701 agcttataac aggaactttt tctcctaacg caaatataat gggatggtgc ttcatcgtga
    41761 cacatatatc tgacccgccg atcaaggccc ttactgttcg gaaatcatag tcatatgaca
    41821 aattgtataa tctatccttc ctataagctt tcataaaatc atttattgct tcctcatcac
    41881 ttggcaccat gggtacaaaa aggatattac aatcagtttt tctcaaaacg atgccgacaa
    41941 ctttgtctat tttctccagt aattgtctct tttcttcgct ggtgtccaag ccccaataat
    42001 gtatgttaat cgctacatag cctttattag cctgttcatc ccttatccct gaattcataa
    42061 gtaaattatt aattttactc tcatcttcca gcttctcgca gaataaagcg tcatcaaagg
    42121 ttgaaaacac atgctcccca cttattccta tttcttccaa agcccttaca gactcataag
    42181 aatctcggag ggttatgagc tttgctttag acagtcccca ttttgacagc cgcttattaa
    42241 agctgccatt ccatacccct atggtttgcc ctgacagaaa taccggtacc ctaaatatat
    42301 cagctattct aataagaaac atactatccc ataaccgaga tacagttttt cctgttaaat
    42361 agcctccacc gctgaaatat atcatgtctg aagtagaaat ctcatatagc aatgctgcct
    42421 tttttgcatt aattaagaat aaaggcagtc ccgccctaac aaaaaaggca ttccaataaa
    42481 ccaataaaga gacaaaaata aactttagct tcttgtaagt actgtccaaa taatacaagg
    42541 cagtctcccc tgcatcgtaa aacgccaccc tcggcgcatc acctacattg cattcgccat
    42601 gtgtaaaata agtataatgc tgatttgggg ttagcacctt tataatgtac ccctcaaact
    42661 cctctttcaa tgaccttatt gtagcgctta actgagcctc atcacctgta tttttgtaac
    42721 cgtatccgcc taccacaagg attttcttat gcttctttgt tttctcatac acaaatatgc
    42781 ttttagcttt aataaacaca gctataaaag gttttagaat tacctcaata cccattgcaa
    42841 atttgctttc cttaaaatgt ttcggttttc cggctatatt ttcaaataat atcctcccgt
    42901 caatgaaatg tactcccggg ataaggccca ttttctcaag ctgcatggat atttggtcaa
    42961 tatacatact ggcaacaata attagaacat catccctatt ctcataaagt attacatttg
    43021 gatcttttat tatggtatta tcaatcatgg tattatgctt tttttcatca ttatcaacat
    43081 agtattcaac gtgcaccgga atagcttttt tcagtgtttc agccatctta ccggttccaa
    43141 aaaaaactac tcttttatta tcagcttttt ttgtaatatc cttaataagt gcttttctct
    43201 taagaaatcc aatgttctct atatttcggg cattgttctc gagcttgacc agtacagcaa
    43261 gcacaataaa tagaatcctc ttcgacaatt catagccaaa atgcctgtaa cagaactttg
    43321 aaagttccga taaaacagtg tgcttaaggt atccactcct aatattgtct atatgccata
    43381 tgggtttatt aagcctgtcc gccgctttag cttgcttgta tagtgttgtg ctcaattggc
    43441 tgccggctac tgtttcaaaa tccaaatatt tgtattcgat attatcagaa tccaaaagtt
    43501 catccaataa tttcttattt ctaatcactg ctatactgtc gcccagtgga ttatctgccc
    43561 acttgcccca tgcatcctta aggcttatgt ctgcagtgta tccccaaaaa tccgagcagt
    43621 aaccgcatac tttcatggaa aaaaagtaat tcctccatat atcagtaaat agagatttga
    43681 atctgttaat ttttactata tcttgttgtc cattgaagaa atgattgttg aaattgtttg
    43741 catctttgat attatcttta ttcctcaaat ttgctttaaa ttgtatatcc tttggtatgc
    43801 cgagcgattc agccagaaaa tcaacaaatt gtccattgac attatggctg caagccaatg
    43861 caagcgtata tattttattc ttattgtatg agctgtgttc tttaaatagc tttgtcagcg
    43921 ctctgattgc acatggaacg ccgataacta agtatgtctt ttccttgcct tttatttcat
    43981 caactatatg tgaataatct atctgcccat aatatgagct tcttttccta tcagcctcta
    44041 caaaggaatt gcttattgat gcttcataat gtaattctcc gattgaacca agtctcattg
    44101 ttgcatggat taccccatca ataattttct tcttaaacaa atgctctact aaagctgtag
    44161 ccactccccc tgaagcactt ttttttctct tgtctgcatc tttgtcatgc gccaataaat
    44221 aagtggcttt ttcaagacca aagctatttg gcgaagatgt ttcacatact ttttgtattt
    44281 cagataaaag cttttctttt gtatgaggac aatattctgt gcaaattccg cacttaatac
    44341 acttggattt atcaatcttg ggatttatat cataattctt tgtcaaaacc atttttatag
    44401 cttcatatgg gcacgacatc ctacaaattc cacaaccgat acaataatta tatttcaatg
    44461 tagataataa aacattgttg tccattagta ccatcctttt tatatatcaa taatcatcta
    44521 ttgttttctt ttaattataa cgataagatg agtacctcca tttatcttga ctaagctatt
    44581 attaatgatg cttgatctaa ccttattata tacacctttt gccttgtagt atggagaata
    44641 cacaggatcc gttaatggag catttttaat ttcaaaatca acatcttttc ctgagagata
    44701 gcttgaaatg ccttttggtg taatggccca caaatgcctt ataggatcta taccatgagc
    44761 tattttatta ctgcaaatag taagtaatgt ctctattctt gagaaattac gattggagct
    44821 ttgtatggtt tttacccggc tttttagtaa ttcagggtat cccagatatt caaaactcac
    44881 accattagga aactgaataa tcagaacccc ggatgcagtc aacagctcaa tacaagagtc
    44941 tatgaactct aaagggtcct taaggtgttc aatcagatgt cttgcatata taaaatcaaa
    45001 ctttaaattc gttctgtcct ttagattctt aacattatca tttatcacat tttttaagcc
    45061 cagttcctgc gctgcaaatt cgcatgcctc attgtcaata tctatataat aactgtctcc
    45121 tttatcaaag tattcaaatg cataagagaa aaatcctccc ccgcctccta catccagcat
    45181 tttcgcatta caacctagat taaatgaatc aaaccactta ttaaaagtct catcttcgaa
    45241 tatactttta ttggatgcat ttgcacaaaa cataaatccc gaatagaaat cttctatttc
    45301 atcatcctgc ggcatactaa gaacagtaga agtcaggcat ttgtcacact gtactatttc
    45361 ataattgttc atattatata tgaaattcat aatgctaccg caaaaatcgc actttttcaa
    45421 aataaatacc tcaattcata atcactaaag caacctacag cacaccgagt ttagacccgg
    45481 ttatttcttc atgcaaatgt gatataaact cgcttacagc gccttcccca cccttgcttg
    45541 acatcacata actgcaaaca ctttttacag ctgctgaagc atcccatggg caagcgctga
    45601 tacctgcgcg ttttaaaatt ggtatgtcat taatatcatc acccatgtaa gctatatcct
    45661 catcttgaca actgtaacta tcaagcaatt tgatgtaggt ttcttctttg ttttctatac
    45721 cctgatgcac ttccaaaatg cccagttctt tagccctaat ttcaacaatt ttcgattttc
    45781 tgcctgtaat aataacaggg attattccca tacttggaag tatatttttt attgcatatc
    45841 catccttaac attaaatgct ttcatctctt caccagaacc ggatatatat aacttcccat
    45901 ctgttaaagt accatcaacg tccataatca acatttttac tctactcatt aaattccacc
    45961 cttaattgtc ctatccaatt ccataattgt aaccagcagc tcctccagtt tgtttagtgc
    46021 tatcatattc ggaccgtcag ataaagctgt atttggatca ggatgtactt caagaaataa
    46081 tccgtcaatt cctattgcgg ttgctgcctg agccagatat tttacatatt cacaattccc
    46141 tccgcttgtt gtccccattg cccccggctt ctgaacactg tgtgtcgcat caaatatcac
    46201 aggaaaacca aatttcctca tttcaactat agatgtcata tcaacaacca gattattata
    46261 tccaaagctt gtgcctctct ctgtcaggag aatattagta ttatcatatg tgagtatttt
    46321 atctatcaca tttttcatat cccacggtgc cataaattgt ccttttttga tattcacggg
    46381 cttttttgtc ctggctgctg cagtcagcaa atctgtctgc ctgcataaaa aagccggtat
    46441 ttgtataata tctgccacat cagagacagg cattgcttgt tcagccgagt gaatatccgt
    46501 tatcacaggg actctaaagc tactttttac tttatccagt aatttaagtc cattatcaat
    46561 tccgggtccc ctgtaagacg ttatcgaaga cctgtttgcc ttatcaaaag aagatttgaa
    46621 aatgaatgat actcttaatt tttcagctaa cctgcttata tactcagcag tacgcataac
    46681 tatttcttca gattcaatta cacatggtcc ggcaatcagg acaagtggat tattcgttcc
    46741 aattttaaca tcacatacat taactactct tggctgcatt ctgtcacctc taatcaataa
    46801 ttactctttg ctttctatat caccttcagc aatatcaacg catatattct gtcatcggta
    46861 tccataatag ctcttaggct tacattatga agatctttaa ataggatgct atccttcaca
    46921 aaatgccatt gattgctttt ttcattaaaa tattgaactg caatatcccc tgtatataac
    46981 atcattacac gagactcctt ggctaaagaa gcatcaatgg atttaaaata gatcactcct
    47041 tgatttctgc ataactcaaa ccttcttaaa atcaatcccg gattagtaca cgaaaagacc
    47101 tgattatctc ttgaattaca ggaaacatta atactacagc tctcatcctg ctttagtcca
    47161 ctatgataga tcttgctgcc attctgccct aacctaaaat aacctttttt caagtcaata
    47221 tagcagtcaa cctcgttacc ataattaggg gtgctaaata ccatatgttt atgttctgct
    47281 tccgttccgc tgtactctat aatatgaata cttgaggtac ctataaagat accggtcctc
    47341 ttgaattcag tacttctctt tctctcatta tcccatggaa taatcccttt caagtacata
    47401 tcatatgttt cataagcata tttattacca agccaaatac acgcctctat tttctcatgt
    47461 atatcaaaat taagatgtat cactggtgtt tcatccggca atactggatt attcaagaaa
    47521 acctgcgacc tttttataaa gtctccaatt tgttcatcaa cttgcggtac aaaaattaaa
    47581 gtttcatcat ctcttgatac tgtcccaaat ctatagagta agagattatc aaaaccaatt
    47641 tttttacacc tactataacc taattttaca caattgttct ctctgtcgaa aagatatgag
    47701 tcagaaagcc tcgtgtctat ttcctcaaaa atatcatatt cctttagtac tttttgtcct
    47761 gacaatatct tatccttatg ctttataaaa aagtttttca tattattccc tcggcaccct
    47821 taattcaatc tattaacata ctccatatac ttgacttttc tactattgag cacatatctg
    47881 acctgaattc cttaggcttg ttactgggca gcgtaatatt tgataaatgc tgaatagcaa
    47941 acatatcatc aaattccacc gcatactgtg cttcatctac agtcatcagc tcctcataca
    48001 tcttctctcc cggacggagt ccgacaaccc ttgtgttaac ttcatctttt gctattccct
    48061 tctcgctgca caccttatcc aacaccactt cggccaagtc tcccagacgt atgacaggca
    48121 tctttaatac gaatatttct ccacccttgg caatctcaca tgcccttaag gtaagattta
    48181 cagcagaacc tatagatatc ataaatcttg tcatattggg atctgtcacg gtcatctctt
    48241 tcctgccgac aatctgctcc tcccagagag gtactacaga gcctctggag ccgattacat
    48301 ttccaaacct taccgcggca aataccggta tctttccgcc cttccaatag tctgcagatg
    48361 caataagcct ctcagccaat agtttggtgg cgcccattgt atttgtaggg gatactgcct
    48421 tgtcactgct tgcatatatt acattcttaa cattgttttc tattgcacat tcaatcacat
    48481 tctgagtccc caatacattt gtctttaccg cttcaaatgg attgtattca catgagggca
    48541 catgcttcaa tgcagccaaa ttaaaaacaa tatcaattcc gtgcatcgct ttgtagatac
    48601 gttctttatc ccttacatcg ccaagcaaaa aacgtatatt cttatagccc tccagctgtt
    48661 gctgcatctg atattgcttg aattcatccc ggctgagaat tcttattact tctgggttat
    48721 aggatataag cttgttcacc aaagcctgtc ctatcgtacc tgtcccgccg ataacaagta
    48781 ttttcttgtt ctcaaacatt gttccacccg ctttgcatta aatactccat atttataaat
    48841 atcgacaaaa tccaactgtt tctttatatt atataagcat tcgtcatatc gcgtactgta
    48901 acacaattga aaaaacccca aaacattttg aggttttaag agcataattt aatagtaaaa
    48961 gtcattattt ttatatcgtt atccataatc gcttcgtaca tatctccaaa tagtggtgaa
    49021 atcaggtgca tatcattgat gcagccatca ataaacggct ccatctctct acacagcttt
    49081 tgtatcttga gtacaatatt cttttcagtg tttatactgt tgatttttct attcaacaat
    49141 gaacattgat aataattcat gggatatatg aatgtactaa aataagtgtt ttgtaacatt
    49201 ttgttaaatt ctgcataaaa aaatgaaaac aatgattccg gagcctctac tgagtttgta
    49261 ttcccattta tttgtgcagc gatttttttt atactcttta atatgtcaag cagatgtctt
    49321 tgctcagctt caattgattt gcacctgctt atcatatagt caatatcata agatacgccc
    49381 tcgtccttat accattcagc atcaactata ctgcttacaa gcatattttc cattacatcc
    49441 ttcaacggct tatataccgt accgccaata gctgcaccac ctcttgtggt atttattata
    49501 ttttcaatgc catactgctt gatgcatctt tcaaattcat ccttgtaatg gtttaatact
    49561 ttatttgttt gaatcttatt tccctctaca tcatctacca tgaagctgct gtccagatac
    49621 ttgttgtctg tttccgataa atccctgcca taatctatac cctttgcata aagtttcctg
    49681 tctttatatg caagattctg acctaccaga ataatcgggt cgcagccaag cttgtaaagc
    49741 atttgcatag ccgctacagc aattgatcca gcatcatgaa cagtatcaaa ttctttagca
    49801 ctttccggtt taaaaaagta tggtgctaca tgatcattgc tgctgataaa atgcagcttt
    49861 ggtccttcat aggcttgtac agtaccgtaa cctgcaatgg tgccaaacac tacaggtatg
    49921 cttgttatgc ccttatcctt cagctttttg aatactatgt ggttgtgatt ctgtggatca
    49981 taggtcaagg ctgcatccgg atatatgtca tattccaaaa gtgtatttat tgctgagccc
    50041 acagataaaa cataggccag tccctttttc tttatgtatc ttaggttttt aagctcatat
    50101 tccagtgaag ggcctgccgc aaccaatatt gccggattac ctgcaaattt attattcttt
    50161 aaatgaagta tgttagggct gtctatgatc tccatgaagt ttttcattat atttgttaca
    50221 tagagctttt caaacaaccg gttggtccta ttcagtgttt ttgtatcgtc cagaatgctt
    50281 ccgaaccttt gatagaagct tttgtactcc tggctgaaaa ttctttcgta gcttggcaga
    50341 attacaagaa gaacttcccc ttctatttct ttcaggagtg cctccaagca ttcataagga
    50401 tcctccaggc tgtctcctat aaaaatattg tccagatact ccaagggaat actatccatg
    50461 gatctgtatg atataaagct cttaaaaata ttaaggaagg gctcatatat tgaaaaatgc
    50521 ttattgccaa atttgctgaa aaaggcttca atatggtatg ccattccgat accataaaat
    50581 atcacatggt catatttatc cacatctttg aattgcccgg caaacatttc agcctcattg
    50641 acaggattat acctgctatg tatataaagc ttgttaccgc tattttccac atatagtgta
    50701 tacatactgt ttcttgcatt ttcaattact acatttggac caacgttttc tgttgttttg
    50761 ttcaagcttt cccataggct ttcaaatctt gttctgagta tggcagaatt atcggttccc
    50821 atttccctcg tctccatgac cgtatatttg tttgatacgt tggttaatac ccgtaaaaac
    50881 ctcagtaatt tcgtacctca gaatatcggc aataagtatc atgtctatat tgcccatggc
    50941 ctctttcaga ttattaaact cttcgcctaa tctgtttccg tatgaagaac attcgttcca
    51001 gatactattg ctttttaaaa cactgtgcaa ggctttatta ttatctatat agtatatcat
    51061 attaaggatc cactgtatcc cctcggcaag ctgccccagc ttttctatgc tgacctcttc
    51121 atccccaccg gaataaaaac taccggcaag cagttcaagc tccggaacag ccctatccat
    51181 atatttgctc attgattcaa gaatactgtc tatggtattt tctactgtat caattacaac
    51241 cttgattgtt tttattctat cgatcctctt ggtaatgtac tcaacgtagt tttcatagat
    51301 ctccatatca tcaattatca tacctgcaag gatatattga ggatcagcga gcaatctgtc
    51361 aatattatca aataattcct ttaccgaagc agattcgttg ctatagttat atgtcctatc
    51421 caaaaaccat acctgcatat tataacctcc aagtaataaa atactctatt attgttatcg
    51481 gtaaatattc cacatacctt aataatagac ctgatgccct tttgtgtaaa aaaaaactcc
    51541 ggaaaaatcc ggagtttctg gcttttgtat tactgcagta actgcaatac accctgaggt
    51601 gcctggttag cctgagcaag cattgatatt gctgcctggt taagaatatt gagctttgtg
    51661 aaagccatca tttctttggc catatctgta tctcttatac gggattctga tgcctgaagg
    51721 ttttcagttg ttgtaccaag attctttacc acatgctcca actcgttctg gacagcacca
    51781 agattagctc tttgtgtact tacggtatta attgctgttt caatggttgc caaatctgca
    51841 tctgctgcgg gaactgttcc tatatttgca agattatatg ctgttgcagt tccagtatcc
    51901 ttgagcatca aattaacagt tccaccatta agaagctgtg ttccattgaa ttttgtatcc
    51961 gctacaatgc tggttattgc agaattaagt cttgtcagtt ctgctgtcat gttagcaaca
    52021 tcaccggcag agtacgttcc gtttaatttc tgaacaccca gttccttcat tctgacgagc
    52081 atatcactta cttcattcag tgcaccttct gctgtctgta tcaatgatat accatcttca
    52141 gcatttctct gtgcctgtgc taagcccctt atctggcctc tcattttctc tgatattgac
    52201 agacctgccg catcatcagc cgctctgttg atcctaagac cggagctgag tttctccaat
    52261 gccttgctct gccctgttga gtttacgccc aactgcctct gtgcattcat tgccataatg
    52321 ttattattta ttctcatttg ttcttcctcc ttgatttttt tagccggatt cctttccggc
    52381 aatatattca taagatcaat ccacaccatg ggccctatgc tgttaattaa tcttatgttc
    52441 taatttatat atcggttgga atgttttgta actttatagc atttaaataa ttatttttgt
    52501 tactttttga tagcttcccg cagtccgtca atgtcaagat ccgacatatc aacagacttc
    52561 cggttttcat cctcaacagc ctttatcagt tctttcctga aaatttttat atttttcgga
    52621 gcttttattg caatttttac tttatcctcg gatatttcgg atattattat ttcaatatca
    52681 tctccgataa caacagcttc atcctttttc cttgtcagaa ccagcatcag acatcagact
    52741 cctttactat tctatgacgt atgccgtacc tctcatcatc aagtatctcc tgaacagcct
    52801 tgttattatt cacattcact actatcggag ccttgagatt ggcagtagca tccttgaaat
    52861 tgccgagtat ggtaagtatc gcatacagct tgacatcttc aggcctttca atactcagct
    52921 tctttacagt actttcgctg atttcaatat cataatcctt gagagcgaaa acaggaggta
    52981 tcataatgaa gcatacatcc tctgaatcta aagattgaag gtagctgata aatgtattat
    53041 catcatttat tatagcatat cgatgcgtcc ccggaaagcc tggtatccct tcatcaaaat
    53101 ccactatgtc cttgtcgcta atctcaatct catcgaaaaa ctttgttttt attatcatat
    53161 tttcacctca ttcttaatca ttcgctttac ctaaggaaat caacaagtga gggctgaatt
    53221 atctttgctc ctccagatag agaagcctga tatacatatt cctccatttt aaggttcata
    53281 acaacctccg caatatccgc atcttcattc tttgaaagca gatccttcag attcagtgtg
    53341 tcatccagta tcctattgct tgtcaactcc accctgttca tcctaacacc gatctcggct
    53401 gttattgcat tcacattttc aaactggttt tctatcctgt tcaatgcgtt gcctatgcct
    53461 gcagtatcat cagcaataag atctgctaac aattgatcaa agatagctat catctgagat
    53521 ttatcccctg agcttacctt ggatacagat ttcttattat cactaattcc aatagccgta
    53581 cccgtttgaa cgctgaactc tccgtcagct tcaaaaacta tcttgttatt tgcaaccgaa
    53641 acggtaacat gtccgttcag atctgctgaa gccattattg cagcgctgat gtcaccttcc
    53701 acatcagcaa tatcgttata aggaacattg ggcagattta ttgtagtagt tgctccttga
    53761 taagtaatat caaagctggt tgctgtatca atcggattcg ttgcaagatc cagaaagctt
    53821 cccgtaatta tttgctgctg tttaatgctt tcggcgtttt tcaaatccaa atcggcagca
    53881 actgagtaca gcccaaaaag cctctgacct acaaaattct caccgattct gtctcctacg
    53941 ccgatgttta cttcaataat ctcttcggaa gataatatat ttgtactgtc tcctaaatca
    54001 taggttccat ctttagccaa taggggttta tccgtcttaa agcctgaaaa cagataactt
    54061 cctgcatagg ttgtattccc gatttgtata agttggttcc taagctcttc aatttcttta
    54121 gatattgata ctctctcatc tatagagttt gtctcactgg cagcctgaac tgtcaactcc
    54181 ttagcccttt tcagaacttc agtgatattg cttgcagcca tctcggtagt gctcagccat
    54241 gacagcacat catctgcatt ccttttgtac tgattcattg tcgccacttc ggtattgagc
    54301 ctcaagcttc tcgatacacc aatagggtca tcggaaggat taacaaactt cttgcctgat
    54361 gataggcttc tctgtgtctt ttccattctg ttgaggtttt tgttcagatt cctcatcaga
    54421 ttattaatta tcatagaatt tgtaactctc ataccatcac ctcattatct tcctactaat
    54481 cctaatctgt tcacggtcgt atccagtatg gcatccaaag ttgtaaccat tctggcggaa
    54541 gcattgtaag cctgctggaa ctttaccatg tttgtcattt cttcatccaa ggataccccc
    54601 gattccgaca aacggctgtt atatatatga tctgtcaatg cttgtgcatt cgtagtcatc
    54661 ctctttgctt ggttgctgtc tacagcaaga gcggaaagta tcgactttac aaagtcatcc
    54721 ggagtaccta tcagtgatga aaacatacct ttattttctc taagtgctat aagaagctta
    54781 atgttatcat tattactctc tccgtttccc tgggatgaag cagctatggt agttggatct
    54841 gctataatat cagaattcac cttaaaactt atgcaattta cttcaggaat accgggttcg
    54901 taaaaaaagt tctgccccgg atctccttgg agatcaactc ccattttgtg ctgtgtatta
    54961 aacgctgttg taaatccttt tacatactca ttcagtttat taaggtagta tggtattcct
    55021 ctgtagctgt aatcttctcc gttgccgtca cgtacatcca acaagcctct cagttctccg
    55081 ctgcttatat ttacctcctg attgtccaaa ccttcccaaa ccaccttgct gatttttccg
    55141 cctcctaaat ctgaaacttc tttcgtttca tcctcagtag ctaatttgtt gaaatgtata
    55201 tggtctataa gggttatacc cccaactttt acatccatat aattattgtt attgggacca
    55261 ggcatttcta taactgtaat atttattata gatgaaagtt catccaagag caaagttctt
    55321 tgatccctga ggtcattagc cttattccct cccaactcaa agctgaatat atgcttattc
    55381 agattagcta tctgctctgt caatgaattt acttggctga ccttattttt tattgaaaag
    55441 ttcacatccc ttattgagtt aacaagctca tgtccgtttc tatttatagt agtggtaagc
    55501 atattcgcct tctctacaac attcacccta caggtactgt ctcccggctg ttttgaaagc
    55561 tcttcaagag ctgtgaaaaa ttcgtccata acgattctta tacctgtgtc ggagggctca
    55621 ttaaacagcc cctcaagatt ctcaagctgg gtctgcttga cattccattc ctgataagtt
    55681 ttattctgtc cccagtactt ggtatcaaga taactgctcc tcattctgat aatgtcgtaa
    55741 gtctccactc ctgtacccac tacccctgta gggtccccgt aagtcggcat cgtagccctt
    55801 tgtttgctta tctgtcttga gtacccatct gtattggcat tggatatatt gtgacttgta
    55861 gtatcaagag acctttgact tgcaaagagt cctgaagttg caatattaaa ggatccgaat
    55921 gttgttctca tataatcacc ccgctatctt cctaccaaac cggttctgtt aacaatcatt
    55981 tcaatcattt cgtcaattac attcactatt cttgttgcag cattgtagga atgctcatat
    56041 tttataaggt tggtcatttc ctcatctaaa gatacagcac ttaatgcttg tctgctatag
    56101 tctatctgat ctaccaaaag cttctgtgca tcagctgcgg tagacgcctc ctgaccgata
    56161 ttccctaaat ctgatatgat attcctatag aattcatcat aattatactt tttgtctgcg
    56221 gctgttgttg tatacttttc atccgtaaaa caatcagtgt acctaagggc tgcaattttt
    56281 aatgctatcc tgttgtcctc attgctgtat gcgtcttcgg cagcagcaat cctgtcataa
    56341 ttttcaagct ccggattata agcaattgtt aagagattta tgcctacagc actattatct
    56401 ctatagttta tgaagaaggg tctttgaagt gtatccgata taccgtaacc ggtaatatgt
    56461 acagcgttaa tctcatccgc aatgcctatt acaaactggt caagcctctc ccggaagccc
    56521 cctaccaaaa catcccttga ttcaaagctt gaattaatac ttcctcctga aatatataga
    56581 ctcccattgt caatctccca tacaagcctt acaaatccgc tgtttcccgt atcaggtact
    56641 gtttttatct gttcatatct gctgccttca accaacaaac gcccttccaa agaaacatta
    56701 atgtaatccc cttctataat ttgtatcttc gccaatccgc tcaactcatc aataagaaga
    56761 ttcctccggt ctctgaggtc atttgctgta acaccgtgag actctatctt ttttatatca
    56821 aggttcaggg tcgcaaattc ctttgctatc tcattaatcc ttgctgcatt gtcaatgatt
    56881 tccttatcct tgcttttcct aaaattaagc agcatggtat ccatgttttt tacagtttca
    56941 acaaaggcaa tagcattttc ctttaccatt gcccttgtag tcagaccacc tgtaggcttg
    57001 gaaagctgct cccacgaatt ccagaagttg ttcatcactt cttgaagacc gtcctccgag
    57061 ttatcgctga atatagtctc aagttctctt acacttgaat atctttcttc ccaataaccc
    57121 agtccggtct tctccttctt gtacttgtta tctaaaaact catctctata ctgccttatt
    57181 tcctgtacat caacgccgta tccaacctgc atcaccttgc cgttccccga atgtcccagc
    57241 ttctgataga aggcgtttga gttagaaata atctgcctgg tatatccggc agtattcaca
    57301 ttgcttatat tatgaccggt aacatccagc gccctctgat ttgcaaagac tgcggatact
    57361 ccgatgctca atcctctaaa cgacattaat actcacctca aaatgcgcaa ggctcattgt
    57421 atgcgctccg gtattttcca ttaatcctaa tctatacctt agtatcaata atattctttt
    57481 tgccttgttt gctcttattc ttgcctatat cctcgtacag ggatcccgtc tccataccgg
    57541 atcctgcaat gaggtttatt gaataatcta tatattccag ggactgctct ataagctgtc
    57601 cgtttatgtc atttgcagcc ttcagctctg ataaagcctt ctgaagcttg ctctggatat
    57661 cctccagctt gtttttcagc tcaggcttga gtacttttgc cagttcgtgt acggtcaccc
    57721 gctcactgtt catatccagc gccttacaga gcatggaaat tacatcctcc cttttcctct
    57781 ccaattgtcc tatactaaag atcatgtcct gctccaactt gaccatcttg tccaattccg
    57841 caactttccc tgcaacaatg atgtccttct tcgactttga cagctcaacc aggtcactgt
    57901 aatagccata ttcctcagcc agtatgtcta taactgcatt tacttctttg tcgacattta
    57961 tcatgtatac accccctgga tagaaataac cacagccagg taatggcctt aagctgtggt
    58021 taatttcata tttttaaatt taacctgcgg gctaaaagct tatcagcgat acctttgctg
    58081 tccacttcat aagtgccgtt atcaagcttt tctttaatgg gggcaacaac ttcttctctt
    58141 atgtccggta tctcccttgc tgcctttgct gctttcataa ccagctggaa atccatagcc
    58201 tctttggata tctgcacttc gtcctttttc cccctggctc gggttgattc cggaacatcc
    58261 ttcctggacg caaccttctg attcttgtag actccgctta ccccatcgat tttattaata
    58321 ttaaatccca cattaacacc ccatttgcat ttcatctttc aactatttta tcggatttac
    58381 ttttatatta gtttagcatt ttttaaaaaa ataaacagcc atgttatatc ctcacggctg
    58441 cttcatatcc tattcatata tttgtttaat cgttcttcct tcttgtggcg gtatacattc
    58501 tttctttttc cgccctggtt tgaggtcttg gctcaggctc cttcctgtcc aatccgaatt
    58561 ctctcttgaa gtctttgtta atctgctcct tgcagtaatc acaaaacctt cctgttttta
    58621 ttgctcttcc gcatctctcg cattccagca tgagattgct gttctcagat ttgatttcca
    58681 gcctttcttc cctcaggaac cgcatgattt tctcaacgga aacctctgtt tcctgcgata
    58741 cttccgaaat agtagctccg ggattgtcat aaatgtattc ctttactacc ttgaactcat
    58801 tttcctccga ttcaatacac ctgccgcaaa tgggctttcc tatataagtg aatattttcc
    58861 cgcacctggg gcagtttctt atttcagcca taaaattacc tccttattct cacatctcac
    58921 gtcccacatc tcaattacgt attgctgccg gtcgctgccg taatcacata cactttggct
    58981 gcaccggcat ctttcaataa cttgctgcat tcattcacag tgcttcccgt agtgtatatg
    59041 tcatcaacca gcaggatatt cttatattta ccaatattat acgataattt gacagaaaat
    59101 gcacccctaa tatttaaaaa cctctgatta cggtctaaat tatactgttc tttggtctct
    59161 accatccttt ccaggcagcc cataacaggc ttgtgcagcc tttcccccaa ttcccgtgca
    59221 attatttgcg actgattgaa gcctcttgct tcaagctttt ttttatggat aggcacaggt
    59281 actatggcgt ctattaccaa cctttcattt tcaagccttt ccgccatcaa accggcaatt
    59341 acatttgcaa gctgaaccat accttcgtat ttaagcttat gtataagctt tttacccata
    59401 ccgccgtatt caaagcagga ataagccctt tcataataaa agataccggt cttacagtcc
    59461 gggcaggtgc aatcatcata ctgttcattc agctgtttgc cgcagtgaag gcaagttatt
    59521 ccctgcaggt aatccaggga tgccatgcat gcattgcata cagccaatct cccggctctg
    59581 ccgcatatac tgcattttgg ttttggggga tacaacaggt caagcaaata cttaatcatt
    59641 ccgttacctc tttattactc tgcagcactt agcctctttc tcagtcctga atggcgtgtg
    59701 attatatgat tgttgcttat catctgataa aggaatcgct ctttccccac cagaaccacc
    59761 atacttttcg cccttgtcaa tgcagtatag aggagattcc ttgtcataag catgggaggc
    59821 ccgaaggtta agggcataac aagcaccggg aactcggagc cctgactttt atggactgtg
    59881 agggcatatg cgtgctccaa ctcgtccagc attgtaaaat catatttgac ttttctttcc
    59941 ctgtcaaata ccacttccat atacaactcc tcattgtcta tgctcacaat agtgcccagg
    60001 tcgccgttga acactccctc cccttccgtt tccaagttgt tgatattccg ccacctcatt
    60061 ctgtaattgt tctttatctg catcactttg tccccaactc tgaaaacagc ctctctgtgc
    60121 tgcttttcct ccttgccgct gccccgggga tttaacacct cctgcagcac atgattcaga
    60181 ttcagcaccc cgcacaggcc ttttttcatc ggggacagca cctgtattcc ctcataggga
    60241 tccaggcctg tgtatttggg aagcctgctg gtgcagagtt caattattgt tgcaacaata
    60301 tcctcctgac tctcctgcct tattaaaaag aagtcaccgt tttttgagct taataccgga
    60361 ggttcccccc ggtttatcct gtgggcattg ataacaatca tgctgctttc agcctgtctg
    60421 aatatctcat ccagccttac cacaggcagc actccgctgt ctattatgtc cctcagtaca
    60481 tttccggggc ccacggaggg aagctggtcc acatccccca ccagtatcac cctggtgcct
    60541 tccgctatcg cctttaaaag gttattggca aggagtatgt caatcatcga tacttcatca
    60601 attattatta catcacactc caggggcgac tcttcatttt tctggaaaaa catttcctca
    60661 tcactgtcgc cgtatccgaa ttccagcagc ctgtggatgg tctttgcctc tctgcccgtt
    60721 gcctcctgca tcctttttgc tgcccttccg gtaggtgcgg ccaacagaac ctcaagccca
    60781 tgcttctcaa aaatgcttat tattgtcttt atggttgtgg ttttgcctgt gcccgggccc
    60841 cctgttatta caaggatgcc ttgggttaaa gcctccttga cggcggtttt ttgtttatcc
    60901 gccagctcta ttccttccgc tttttcgatt tccgctattt cgccctccat atccagatcc
    60961 ataggaggaa tcttgtagta caacagctgc ataagccttc ttgcagtccc tgattcagct
    61021 ctgaaaaaag gaacggagta tactccccgg atgtctccga tgttttccgc aaccagcttg
    61081 ttatctataa aaagccttac tattgtatct tctatatggg cttcatcaac gtccagcagt
    61141 gctgccgacg atctcaccag ctcttcctgg ggcacaaagg tatgtccgtt tccatgatat
    61201 tgattgagca catatcttgt acctgatgca gtccggtatt ccgagaactt ggacaccccc
    61261 atggatattg ctatcctgtc cgccattttg aagcctatgc cgaatatgtc atccgcaagc
    61321 ttgtaggggt tttcctttat ttcttttatt gtgtttgctc cataagtcct atatatcctt
    61381 acggcataag cgggccctat gccgtactgt tcaaggaaca tcatgatatc cttcagctct
    61441 ttctgctcct gaaaggcttc ggatatcttc gccgcctttt tatctcctat cccctccacc
    61501 tccgtcaggc ggtcgggatt gtactgcatg atatcaagag tatcaaggcc gaacttctca
    61561 accagcttct tggccgtatg ggggcctatg cccggtatca tgccggagga cagatacttt
    61621 tctattccgt taattgttgc cggagtaaca gtagtgtagg tctccacctt gaactgactg
    61681 ccgtaactgg gatgctgcac ccatgaaccg tatgccttta tgatttcacc aatattcagg
    61741 aaggaaaaat atcctatcag cgtatgcagt tcattattgc actccaaatc cagcactgta
    61801 tatccgttat attcatttct gaatatgatt tcgcatatgg ttccttgaac ttcatccatt
    61861 gtatcatatc cctgctaatt ttttcagctc ttcgacttta tcggtcttct cccagggcac
    61921 atctatatct gttcttccca tatgcccata tgctgccaac tgcctgtata tcggccttct
    61981 caggtccaga tcctttatta tggccgccgg cctgagatca aataccttca ttattatttt
    62041 tgctatctca tcatccgtca gcttgccggt tccgaaggtt tccaccatta ccgacaccgg
    62101 ttttgccata cctattgcat aagccacctg tacctcacat ttggatgcga ggcctgcagc
    62161 aaccacattc tttgccacat accttgcggc ataggaagca gatctgtcaa ctttggtcgg
    62221 gtcttttccc gagaaagctc ctccgccgtg ccttgcatat cctccatagg tatcaacaat
    62281 gatttttctc cctgtaaggc cggaatcccc ctgaggccct cctattacaa accttcccgt
    62341 aggatttata tagaactttg tatccttgtc taacattccc tccgggataa cgggttttat
    62401 aacattgtcg ataatgtctc tctcaatagt gtcatggtcc acctccgggc tatgctgtgt
    62461 ggaaacaact attgtatcta cccttacagg ccttccgtca tcgtattcca cagtaacctg
    62521 ggtttttccg tcaggcctaa ggtagtcaag gatgccttcc tttctgacct gtgaaagcct
    62581 caaagcaagt ttgtgtgcaa gggaaatagg catcggcatc agttcaggag tttcatcgca
    62641 ggcaaaaccg aacatcatgc cctggtctcc cgctccggta gcagctatat cctgctcagt
    62701 catctctccc ttttttgctt caagagcctt gtcaacgcca agagcaatgt ctgaggattg
    62761 ctcgtctatt gaggtgataa ccccgcaggt gtcgcagtca aaaccgtatt ttgcccttgt
    62821 atagcctata tcctcaatgg tctttctgac tattttggga atatcaacat aacattcggt
    62881 agatatctcc cccattacca gcacaaggcc tgttgtgacc gatacctcac aggccactct
    62941 tcccatggaa tcctgttcaa gtatcgcatc cagcactgca tcggatatct gatcgcatat
    63001 tttatcagga tgcccttcgg taacagattc ggatgtaaac aattttttta acataataaa
    63061 cctccttaag cattcgttct ttaataggta taaaaagaaa actccttctg acgaaggagc
    63121 atgacattta ttatttactc cctcatcttt caggattgat aatgacaatc ctgcaggaat
    63181 tggcaccgta catctgccgg ttgccgggtt tcatagggcc cttccctcca cctctcttga
    63241 tgagtcttca gttataaagt taacagcatt atactatcat agcaaaattc aactgtcaat
    63301 agtaagacac gggggggaca cggggacgtt tctcttgtcc cgccctggac taaaacattg
    63361 aaatctgctc atataaatag ttttgctgat attcattaat tatatccgcc attttataca
    63421 aaataccaag cctgtcacac cgggccctga aaaatttcca cagctccttt gctctgggag
    63481 atatgcattt ataggaattt ccgtattgtt tgacatattt ttgcctgatg gacggaaaat
    63541 gttcatcaat tttgctgtag aaccattctc tctgattttg ccgcagggta actccgaagg
    63601 caggatatat gaattttgcc cccttctcat atgcgagatc tataatattg cttatattat
    63661 cctcattatc ctccagaaac ggaagtaccg gcataagcag tatacccgtg aaaattccgt
    63721 tcatcgaaag atccctgaca gcctcaaacc tcctggaagc cgcgggagca ttaggctcaa
    63781 tcttactgca aagcgaatca tcagcagttg taaccgtatg cttaacgagc accggagaat
    63841 gccttttaat agaatttaac aaatcttcgc tgcggctgtc gcaatatatg cagccatggc
    63901 agcagccctt gtagatattc atgctgtaat tattaccaaa ccacgggttg ttttccgtat
    63961 agccggagat aattgttttc gcaggtataa attccatatt ataaccctct cttttggtac
    64021 atgggtggta cacggggacg tttctcctgt cctattttac agctttcgct ataatgtttt
    64081 tatttactcc aaatacgcgt tctaactgcc tgatgcttgc tcctgtacta ttatatatat
    64141 ccttaatcag tctatttcga tctcctattg atttcccgta caaaaaaaca gaacctattt
    64201 ttttcttaat tatatctccc agtgcctcat ctgtaaattt ttcttccggt tcaaaatcta
    64261 caaatatatc atcattttgt gtgtttgtga attcctcaaa atcattctga tctttgaaat
    64321 atgccataat catatcagca tcaatattta cgccattccc ttgatatgcg gaaatataat
    64381 cattatagct gctccacgga taatcttcag gtttttttac catattagct ttcaatggat
    64441 tctgatgtat gtacctcagt acatggacca aatagccttc agattcgacc ggttcgctta
    64501 aatacctgtt ttgaaacaga tgccccactc ttccgtatct attattgtgc cacgatgcat
    64561 agcctacgga tacacgcttg atgctaactc ctatttcttc actttcgagt atcagaagat
    64621 gtatatggtt atccatcaaa caatatccgt ataggccaaa ttttgcattt tcttttgctt
    64681 ttagaagttt tttcataaaa atcatcttat cttcgtcatg cagaaaaata tctctcttat
    64741 caattcccct tagcatgaca tgataaacac ctgttgcgct tcttttcctg gcctgtcttg
    64801 ccatatcaaa ccctcccaag ttcatcattc ggaagacagc ttaaacgtcc ccctgccttc
    64861 tttcctaatt ataccaaaca tatgttcgta ttgcaaccat tttttaggaa attgtcacca
    64921 atgggacagg agaaacgtcc ccgtgtcccg ggagaaacgt ccccgtgtcc cgcaaaaaaa
    64981 gaaaaaagcc ctttcggact attgttttaa aatggtgggg aaaggtggat tcgaaccacc
    65041 gaagtctgcg acaacagatt tacagtctgc cccctttggc ctctcgggaa tttccccatt
    65101 atggagctgg tgatgggact cgaacccgca acctgctgat tacaaatcag ctgctctgcc
    65161 aattgagcta caccagcata atttgcctcc gcttatgctc cggctttaat caatgaatgt
    65221 cggtcttttt aattttgcta aacagtttgg ttaaagctgc tccgcaaatg ctgcgcatta
    65281 cactaaggaa tgtcggtctt tttccgtgaa gcggaaaaag ttggtgaccc atgggggaat
    65341 cgaacccccg ttaccgccgt gaaagggcga tgtcttgacc gcttgaccaa tgggccatat
    65401 cttgcacaca tggtcggggt gacaggactt gaacctgcgg cctcatggtc ccaaaccacg
    65461 tgcgctacca tctgcgctac accccgacgc gacttctcta ttatataaaa aataagaata
    65521 tcaatcaatt atcaaatttg accgaaaaca actaaaatcc aagattatat ccctcatctt
    65581 gtaatatgct gaggaatgat acttattatt cataattata atttcagcat tctaaaaaat
    65641 caatagacaa atcatccagg aatatgggac atcacggctt tcatcttaca caactcattc
    65701 atctcaatat tatgatatga ataattgacc tgaacttcca atatatttat aacaagatcc
    65761 aacgaagagc aaaaaacaaa tgtttggagg tcatattttg attgagaaat acagcataaa
    65821 agacggaatt tgtgtgcatc atacggataa tcgggcacat ttgaaaatct ctgatttttt
    65881 gaaatcaaat aaattagata aatgcttaat actttgtata ggcaccgaca ggttcatagg
    65941 agattctctc gggccgctga ccggcacatt gctgagcaag ctgagccttc cggtcccaat
    66001 cctggggacc ttggataatc ctattcatgc ggttaatctc agaagggtca tgtctcatat
    66061 aaaaacctcc tataaagatc ataaaatcat cgccatagac gcttgcctgg gtgcgggcaa
    66121 aagcgtagga tatgttcatg tcaaggaagg cagcatccag cccggaaaag gcgtcggtaa
    66181 aaggctctcc catgtgggag acttttctat aattggagta gttgatgaag ctgtggaatc
    66241 aggtgacagt gaatatgaga actacctcaa aataagcaat gtaaggctgg gactggtaat
    66301 gaaaatgtca gaaactataa caaaaggaat atatgccgcc tttcggcagt agggacgcca
    66361 tatatggcgt ccctacttgt ccaaataaaa aaacggtgaa tacatcaccg ttttatttgt
    66421 tgactatcgc aaacataagt tctccggaag ctgcaagctc atcgcccaca taagcctggg
    66481 cttttgcctt gcctatgccc cttttaaaat taagcatttc tacttccatt cttaatgtat
    66541 ctccgggaac aacctggcgc ctgaacctca ttccgtcaat ccccgtaaaa accgccagct
    66601 tgttcttgtt ctcttccatt cccagaattg ctaccgctcc tacctgtgcc atagcttcca
    66661 ctataagcac tcccggcatt atcggaagcc ccgggaagtg cccctgaaaa aaaggttcat
    66721 taacagtaac atttttgata cctaccgctt tttttccata ttctatttcg gttattttat
    66781 ccacaagcaa aaatggatac ctgtggggta aaattccttg aatatccgta ttattcaaca
    66841 tcttccctct ctctttccgg cttcaggctt tttgacagct gctgataaag cagatccgcc
    66901 agccctattc cttcgccctt ggaagcttcc tccgcatatt tgtcatacag catttcctca
    66961 tacatttcct ctgccatacc gccgtcaatt aaaccggctt tctgcaccgt actccgcatt
    67021 tgcttaaaga ccatgctgac aaatatagcc tccaggtcgc tgcaagcttg cttaagcttc
    67081 ttctcgtcct tttcctccat tgctttttca agagctttcc caaactctcc ctgctttgct
    67141 tcctgagctt tgcttttagc attattataa tctgttattc cattattatt aataggatta
    67201 atcataatta tctcctaagg ttatttgcca tctgcagcat ctcgtccgct gtctgtatgg
    67261 acttggaatt catttcatat gccctctggg cctgtatgag atttaccatt tcctcaacaa
    67321 tctgcacatt tgagtactcc aggtaaccct gcaggatatt ccctccggtg ccgtcgttct
    67381 gggtatcgaa ggcttctccg ctggctactg ttgctacata aaggttcttc cccacactct
    67441 caagccctgc aggatttaca aaggttgata ttgaaaggct ccccagctct tcaatggtgc
    67501 catcctctct tttaacagtt atcaatcctc ttgcgtctat tattatatcc ttgatatccg
    67561 aacccaattc tatattgccg ttctctcctt gaacataata gccttctgag gttaccaggt
    67621 tgaagttctc cccatccggc gatatcttga aagctccatc cttggtataa tactcctgtc
    67681 cttcaggccc cagcacctta aaaaagccca aaccttcaat tgccacatcg tacgggtttt
    67741 cagtggaagc caggtttccc gtagtgaaga atttgactga agcggcgggc atgacaccgt
    67801 gccccacctg cagattgaca ggtctcccct gtccgtccct tactattcct ctttccaatg
    67861 tttcataaag caggtccttg aattccactc tggacttttt atatgaaact gaattgacat
    67921 tagccatgtt gttggatatg gtatcaacat taagctgttg ggccaacatc cccgtactgg
    67981 ccgtccaaag agctctcatc atagtttatt acctcctaaa ccctcgcaat ttcatttaca
    68041 gcttttccaa tcagttcatc atgtatcttt attatccttt gattagcctc ataggttctc
    68101 aacatagtca gcatttcaac catttccttt acggaattga cattggaacc ctcaagaaaa
    68161 ccctgctgta ctgttcccgc cgttattacc ggttccgcct caatttcttc tgctattctg
    68221 atgaggccgt ttccttcctt gcgcagcgcc tcataatctt caaagtccac aagcctgagc
    68281 ttgcctgcca gctgtgcatc ggaaaaaacc tcgccctttt catttactgt catatcgctg
    68341 ccttctatcc ttatgtatcc gtcttcgcca aagaccctcc atccttcccc ggttaccagc
    68401 cagccttccg catcctttgc aaagctgccg tttcttgtat acctttcacc ctcaggggta
    68461 tcaatacaga aaaacccttt ccctcttaaa gctaaatcaa agctgtttcc ggtagagttc
    68521 agctgcccct gtttaaaatc aatcattatt tcactgctgt gtattccata gcttaaaggc
    68581 cccaatggtt taaagggcct tgaaaccttc aagtccttgc caaacctgtc atttattctg
    68641 gaaatcagaa cctcggaaaa gttctgtgtc accatgacat ccttcttata tcctgtagta
    68701 ttcacatttg caatattgtt ggctattaca tccactcttt ttgcttcagt aattgctgag
    68761 gaggctgaaa tataaagtcc tctgatcaca ctatcaccta cattaaatgt tattctttac
    68821 atgaaagtta tcggtataat gtttaaagga ctttagcaga taaaaaaacg gggacgtttc
    68881 gaagggacat ggggacgttt ctcctgtccc gctaaaagcg ggacaggaga aacgtccccg
    68941 tgtcccttct cttgtatctg acttaataat tcaatgtgat aatttttctg tttgcattta
    69001 tgtccgaatc ctgaagcatg tctatatgct ccaaagcctg ccctgttcca atagctacgc
    69061 aggatattgc gtcttccgcc acatatacgg gtatgccggt tcttttgctt atcagcttgt
    69121 caaggccgtt taacagggaa cctccccctg ccataacaat gcctctgtcg cttatatcgg
    69181 cagaaagctc cggcggcgtc ctttccagca ccgaatgtac cgcgtcggca atggcggaaa
    69241 ccggttcgga aagcgcttca agcatttcat cagaagtaac ttctatggtc tttggaagtc
    69301 ctgaaataag atttctgccc ctgacttcaa ccttaagctg ctcttctctc gggaacgctg
    69361 taccggcgtt tatcttcagc tcttcggcag ttctctcacc aatcataata ttgtgcttct
    69421 ttctcatata ccttacaatg gcttcgtcaa atttgtcccc tgcaatctta atggaggttg
    69481 atacaacaat tcctcccagg gatataaccg ctatgtctgt tgtccctccg ccaatatcta
    69541 ataccatatt gccgctggct ttggatatat ccaaacctgc tcctatagca gatgcgattg
    69601 gctcttcaat aagccttgtg gttcttgccc ctgcgttatt ggcagcatca ataaccgctc
    69661 tcttttcaac ctcagtaaca ccgctgggca cacaaacaac catccttggc ttaattatcc
    69721 ttctcctgcc gatagccttg tctatgaaat atctgagcat tttctcagtt atgtcataat
    69781 cagaaataac gccctccctg agcggcctga ttgcaacaat attaccgggt gtcctgccaa
    69841 gcataagcct tgcctcttca cctacagcca atattttgtt tgtattcctg tctatagcaa
    69901 caaccgacgg ttctctcaga actatgccct ttcctttaat gtataccaac acgctggctg
    69961 ttcccatgtc gatgcctatg tcttgtcctc cccacatttt aatacctcca taatatttcg
    70021 cttgtcatta tcaatccttt tatatttcta tataaaaaac aaaattcctt cacgaagtac
    70081 attattataa caaaaaagta gtgactattt tatggcttgt ctactgcaat tataagacaa
    70141 ataaccccaa ataacaagct ccggcaaata taatgtgcaa tgcagcagca tcagcacatc
    70201 aatacttgcc ggagcctgaa ccttcatcac tacaaactta tggactgata tttcatcttg
    70261 gttgcccttc cgcctcttat atgtctttct gctttgttct ggtccattat ctccttaact
    70321 tgaagtgcta cactgggatt tattctcgga agtctttctg tgacatcttt atgtactgtg
    70381 cttttactta ccccgaatat tttggctgca tctctaaccg ttgtcttgct gcttattata
    70441 tatttcgcta tttccaatgc tctttcttcg atataatcct tcaattacta ctaccccctt
    70501 attggtgtct ttttacatta atatgcggtt tttttggtaa ttagaaccac acgtatcgtt
    70561 cattaattaa tgctttttga tgcccggccg cactatttct ttagtgcttg tttgggcagg
    70621 tacttctttg gatcaacact cttgccttgc ttgattactt caaagtgcag atggggatcg
    70681 tcggcaattt catacagggc ggttttccct acaccgctga tcacatcgcc ttttttgaca
    70741 ctctggttta tgcttaccaa atcgagggta gaaaggttgc tgtatttagt gtagacttcg
    70801 ccgccgtgat ccagtacaat ggttaatcca agctggggat catttacaac ctgatccacc
    70861 acaccatcca tcgctgctct tacagggctt ccctcttccg cctttatatc gattcctgca
    70921 tgtgtactcc actgatccag tgtttttgag taaacaagca catcctctga aaattccgta
    70981 tacatagtcc caaatacagg aaccaccatt gtctccattg ccggctcttc catttttatt
    71041 gcagaaatag gcttggcttc attgactttc tctttggtat cctttttatc ttcggttatc
    71101 tttactgaac tgctgtttga tacttctgtt gcatccggtg tttctacaac attttcactt
    71161 ccgccgcttt ccgccttttc ctcgattact tgcgctgtag aactgggttc agactcctca
    71221 atgtattcct ccatgctttt ctcaaagtcc aattgtgcct gtttatccaa aaggtagttt
    71281 ctgtactgca ttactcctcc tgcagtaagc aatgcagcta tcaacaagac aagaaaatat
    71341 atatgcctat tattgaattc ctttctaaat ttcataattt acacctccgc cagtattatt
    71401 tccaataatt ttaaaaataa tacaggcatt cggcgaaatt tttaatttgt taaatccttt
    71461 atactcacta ttgcaacccc ttggtaataa tgggttacaa tatccctgta tgatgccccg
    71521 ttttttgcca tgccgtcggc accgtactgg ctcatgccta ttccgtggcc gtttcctacc
    71581 acagtaaaca gaacagtgct tgcggtcaca tcaaaattga agtttgagga gttcaaaccc
    71641 agtgtatttc tcacatccgt acccttaaag gtcttgtttc cgatctttat acgggttatt
    71701 cttcctccct cgctcctctc cagcaccttt atttcattct ttatatcctt tttatttatt
    71761 accacccctg agtcaagctt cttaatacac tgcacaaaat tatcaatgct tatctcctgc
    71821 cgggccacat acttggggga gtgttcctcg tattggctta caacgcttcg caaataagga
    71881 accctgtttg accatatatc ctctgaattc tccgttttcc ccccgctggt ggagtggaaa
    71941 accgcatcga taagcttatt atcatagact attacctctc ccgctgtccc atataccgca
    72001 tccttcagct tatccaggtc cttcttcact gacgccggat ccctgtatgc ctggcagtga
    72061 gtggagtctg tgcatatctc cgctccctgg tgcttgctgc agccgctgcc tccaaactgc
    72121 ctcatcctga gcattgtata agtccttgca gccagtgcct gggctttaag ggcttcagga
    72181 tgaaagctcg caggcatttc tcctgcaaca acacctaata tatagtcctc cagattcatt
    72241 gtcacaacct catctgtatc acacatatag acctctatgg gcagatcctc ccggggcact
    72301 tcctttttcc ccgactcttc tcccttgtct ctcacaagga gcaagggaat ggcaaacagc
    72361 aatatcatta gtactgcaat ggctgcggcc aacttcttca ataatcgtac ccccctccaa
    72421 aatattcata tccatatgca acacaagaaa cgagtaacac gggagtcacg gctgtttcac
    72481 ggaataacac agttaattat tatattttta tcgttataat cgctaaatgg gaaaataccg
    72541 ataccttacg cgttatcgat tattactcga gtaatagaat atttccccgc cgtgtattca
    72601 gtgttatacg ttactttacc cataaggaat accctggctt tcctcgtaac ctcgcagcct
    72661 cgcaacccaa aatctagtgc ctcgcgtctg aagactacgt cgctttttcc tgcttatgtg
    72721 ccatatctat atttattttt aaggaggcca ttatatgata ggtgaaatta acaaaggtga
    72781 ttgaagaaag ttatgccttt ctatacaata agaaacccgg tgctgctgca ccgggagtta
    72841 ttattcctct tctcttctta tatccgcacc caactttttc aatttttcct caatccttat
    72901 ataccctctg tccaaatgat atacatcatg tatttccgtt cttccctccg cacagagtcc
    72961 ggcaatgacc aatgccgccc ccgctctcag gtcggtagct ctgacctttg ctccgctgaa
    73021 cttttcacag ccctcgatta ttgcgcttcg cccatctacc tttatatttg cccccatcag
    73081 cttcagttca ttgatatgca tgaacctgtt ttcgaatacc gtttcggttg caatgcttgt
    73141 tcctttggcc aggcacattg ctgtcatcat ctgagactgc atatctgtgg ggaatcccgg
    73201 atagggcatt gtcttaatat ccactgaatt caaccgctcc gctgccctga ctctgatagc
    73261 cctttcatat tcctgtactt cagcacccat ttccttcaac tttgcagtaa ccggcttcaa
    73321 atgctccggt actatccctt ccactatcac atctcctccg gtggccgccg ccgcaaccat
    73381 atatgtcccg gcctctatcc tgtccggtat tgctgtgtgg gcagtaccct tcaattcttc
    73441 taccccttcg attcttattg tatcagtgcc ggctcccttt attaccgctc ccatcttgct
    73501 taaaaaactt gccaaatcca ctatttcagg ctcctccgcc gcattttcaa tggttgttgt
    73561 accctctgcc aggcagccgg ccatcatcag attctcagta gcgcctacgc tgggaaaatc
    73621 cagatatatt gtctctccct tcagtttttc agcatatgcc tcaatatagc cgtgtcccaa
    73681 tgtaatgttt gcccccatca gggaaaatcc tttaagatgc aggtctattg gccttgtgcc
    73741 aatggcgcat cctcccggaa gagaaatctt taccttgcct atccttgcca gcatggggcc
    73801 cataataaga aatgacgctc tcatcttgcg caccagctca taggttgcct caaagctgct
    73861 tatgttttcc gcttttataa ccaagctgcc gttggtccgc tccgcttcag ccccaattga
    73921 ccttacaacc tcttccatta tatctacatc atccagacaa gggacttcgt ccagcacaca
    73981 agtatccggc gtcagcaggg acgctgcaat tacagggagt atggcatttt ttgcactgtg
    74041 cacctttact tcacctttaa gtacacattt atcttcaatt gttaattttg tcaaaatttt
    74101 tcctccctaa tatcccatag gtatcaacgg cgtacctatc cagatgtagg ttctgtcgtc
    74161 ataggaactg taccgcatgg ccagttgtac attcacttta gcctcatccg acaccacatg
    74221 ccctcctaca ctgctgctga aagcggatat gcttttaagc tcttcgtttt ccattccttc
    74281 cacttccctg gcattgatgg actgcagtgc catccttgac tttttatcgg cgaccttctc
    74341 gtccagcctg cctttataag tgcctataat gcagctgctg aaattcattg agccttccgg
    74401 cctctgcatt gcatcaatta tattccgcct ctcggctata agctcctgat agcttctgtc
    74461 tatggagaca tcaataacag cataggtctc tccaatccct tcctgtttta ttgactctat
    74521 tgctatacta taggttctgt tacccttgaa gcaggacacc actatcttat ttaaaccgct
    74581 gtcgctttca tcgtttttaa caaccgtaac cttgtccggc tcaatccgtc ggactatcct
    74641 gttcatttcg gcctctatct gctctatatc caaaaatttg tcattaagct ttgtccagca
    74701 gcttatggtg ctttccagca catccgctcc ggtacttctg aatgaagtaa caagtgcctc
    74761 ttcttcggta agagcttcaa aaggtttttc cgcggcttca aaactcccca ccgaaaataa
    74821 agcacatact atcaacagca aaattttctt cctcatatcg atacctccca ttcttttaaa
    74881 ttattgcccc atgggaggat ttatattcag cttaattaaa cgaaaaagac gacaatttat
    74941 ttgccgtctt cttctctctg tgctatctga ttttacacgt tcataacctt tagattttct
    75001 gatataataa gctctgcctc tgttgcactt attacttctt ccaacgttgt ctcgggtgcc
    75061 agttctgtaa gaagcagtcc cttgggtgtt acttcgataa ctgccatgtc agtgactata
    75121 aggtcaacct ctttttcggc agtcaaagga agattgcatt ttttcagtat cttcggagcg
    75181 ccttttgccg tatgctccat ggatattatg accttcctgg ctcccgcaac aaggtccatg
    75241 gctccgccca taccgggcac cattttaccg ggaatcatcc agttggcaag atttccttcc
    75301 tggtctacct gaagagcacc cagcacggtt acatcaaggt gtcctcccct tattatggta
    75361 aaggacatgg cgctgtcgaa aaatgcccct ccgggaacca cacttgccgg aagccctcct
    75421 gcatttacta tatcgagatc ttccttcccg ggttcggggt tcggccccaa accaatgaag
    75481 ccgttttcag aatgaagcaa tatgtcaatt ccttccggaa tataattagc taccaatgta
    75541 ggcattccaa tgccaagatt gaccagatcc ccgtccttca gttcctgggc aactctcttg
    75601 acaataactt ccctttttct atccttatcc tggatcactt gattcactcc tttacatatt
    75661 tattttgcaa ttatgtccac aaatatcgac ggggtcatca cgtcagtcgg atccagttct
    75721 cccacttcaa ctattttctc agcttcaact atgaccaaat ccgctgccgt cgccatgaga
    75781 ggattgaaat tccttgtggc cttcttataa taaatgttcc ccttcttatc aacaatgctg
    75841 cctttaagca gtgccacatc agccttcaaa ggaagctcca ggaggtattc cttgccgttt
    75901 acggttatct tctccttgcc ctcttccgcc accgtaccga tgcccgtggg ggtaagtatt
    75961 ccgccaagcc ctgctccggc cgctcttact ctctctgcaa gggttccctg cggaaccagc
    76021 tcaacctcca gttcgccggc attcatctgt ctgccggttt cgggatttgt ccctatatgg
    76081 gatactatta tcctgctcac taccctgttt accacccact tgcccactcc cttgtcaggt
    76141 aaggcagtat cgttgcaaat cagcgtaaga tcctttatac ccttggcaat aaccgcatca
    76201 atcagctttt caggagtccc cgcaccgata aatcctccga ctgcaacagt catgccgtcc
    76261 tttatatagc ttgctgcctc atcgattgtc ctgattttgt ccacaactcc acctccaaag
    76321 tattaat
//
LOCUS       DAOQUR010000007        67355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_477_length_67355_cov_9.975319, whole genome shotgun sequence.
ACCESSION   DAOQUR010000007 DAOQUR010000000
VERSION     DAOQUR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 67355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67355
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_67355_cov_9.975319"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1164
                     /locus_tag="PKI50_02585"
     CDS             112..1164
                     /locus_tag="PKI50_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT02634.1"
                     /translation="MNNIWGSKKVNIRLIHIIIIAALVFIVLKYGIAISTVIKPFIIA
                     AAFSYLLNPIVKVFEKKGIRRIFGVLIVYLIFIAIIMLLSFVLVPKLIKEISTLVVNI
                     PQYSRQMQELFRQFQDGYMRSGLPESFKDVLDDNILMLQSMILTLLQNIANGIIEIFS
                     QFFNIVIVPVITFYMLKDAEYFKNQFILVLPKAKRAKLIILIRDIDNVFGKYIRGQII
                     IASFVGVLTTVALILIKVKYAFILGIFAGIANIIPYFGPFIGIIPTILFALLDSTGKA
                     LYAAGVFILIQQLESGFLTPRIIGKSVGIHPVYVIMSLIAGGRLFGIIGLIMAVPVLA
                     AIKLTLRHVFRDRQGS"
     gene            1521..4160
                     /gene="alaS"
                     /locus_tag="PKI50_02590"
     CDS             1521..4160
                     /gene="alaS"
                     /locus_tag="PKI50_02590"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNT02635.1"
                     /translation="MEKLGLNEVREKFLSFFESKDHLRMKSASLVPQSDKSLLIINSG
                     MAPLKPFFTGQAVPPSLRVTTCQKCIRTPDIERVGKTARHGTFFEMLGNFSFGDYFKD
                     EAIAWAWELCTEVYKMPEDRLWVTIYLDDDEAFDIWHNKIGLPPDRIVRMGKEDNFWE
                     IGLGPCGPCSEIYFDRGPGTGCGKEDCKLGCDCDRYMEFWNLVFTQFDKDEEGNYNRL
                     AKPNIDTGMGLERMAAVMQGVDNIFEVDTIKYILDSICSLAGVEYGADPKKDVSIRVI
                     TDHIRGVTFMISDGILPSNEGRGYVLRRLLRRAARHGRLLGVTDSFLSKIALKVIEVS
                     GDAYSELREKKDYILNIIDVEENRFYETLDQGLSILQGYIDELKLTNTDTLSGENAFR
                     LYDTYGFPLDLTKDILEEQGLSVDEETFQKEMQNQRERARAARADSSAESWKEDIYAK
                     IDWSIKTKFVGYESFSAKAIVKAIVRDNELEDSANEGDEISVVLDITPFYAESGGQVA
                     DTGRIIGEGFELDVKDCKKVTGERFAHVCTVISGEIKVGDSCIAEIDSKRRMATARNH
                     SATHLLHKALRKYLGTHVEQAGSYVTPERLRFDFTHFKAMTKEELDRIEREVNEAILA
                     SYEVITKETSINEAKKLGAMALFGEKYGEVVRVVKMGDYSIELCGGTHLKATSQAGFF
                     KIISEGGIAAGVRRIEAATGEKAYEFIKEEEKLMNELCGKLKAHPSECVKKMDTVLKQ
                     LKDTEKEVSVLKSKMAAEMLDELAAGAQEEKDIKYLVTKLDNMGIDSLREMGDKLRDK
                     LGTSFVMLASFDGEKTVLVAMASKDAVSKGVHAGNIIKEAAKAAGGSGGGRPDMAQGG
                     IKDLDKVAAALDAVPEILKNMVK"
     gene            complement(4359..5675)
                     /locus_tag="PKI50_02595"
     CDS             complement(4359..5675)
                     /locus_tag="PKI50_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT02636.1"
                     /translation="MSKPVLSAHFESRTPSSVRLAQMKYEERKVKPQAVINVGIGNVS
                     LPTNPAMQKRMFSLDAPESPFAKGVIRYTTTAGLIECQDAFKNILKCEGFDTSKLYVQ
                     ITDGGSAAMELLLLGVCGPAGTDEKPLMVIDPAYTNYISFAERVGRKIVTVTRSLNEN
                     GKFSLPKLNEIEETIKKYNPGALLVIPYDNPTGQFYDYETLKGLAALCVKYNMWFVSD
                     EAYRELFYEEGSKLVSIWGVTDADVPGIEGRRMSIETASKVWNACGLRIGALITDSPE
                     FNNRSVAEYTANLCANAIGQYIFGALAHESKEQIAAWCKSIREYYKELIFNVYNGLKK
                     QEPGLIVSSPDASIYSVIDVRNVVKPGFDATEFVLYCAGEGSVNLDGVETTLLVAPMK
                     GFYNTKKGEYNPGSTQFRISFVETPDKMAKVPELFAKLLRQYEAQR"
     gene            5921..8407
                     /locus_tag="PKI50_02600"
     CDS             5921..8407
                     /locus_tag="PKI50_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT02637.1"
                     /translation="MKAVIMAGGEGTRLRPVTCGIPKPMVPVLNKPVMEYTIELLKKH
                     NITDIAATLAYFPAVITDYFGDGGEFGVSLRYYIEHTPLGTGGSVKNAEDFYDDTFII
                     VSGDALTDIDFEKALEFHKYRKSKATLILKKVPVPLEYGVVIIDEEGRITSFMEKPSW
                     GEVFSDTINTGIYILEPEVLDYYSKGDNFDFSKDLFPKLLRDNIPMYGYVTDDYWNDI
                     GDLRVYKEVNFDILSGKVKTTIGYRQHGEGIWIGDGCEISDSCNLIAPVLIGDNCRIK
                     DRTVIEASILGNDTEVQEATSIKKSIIWKNSSIGKNVQCRGGVICSGAAVKSGVHIFE
                     NTVIGSNTTLETGAIIKPDIKIWPEKVVEEDAVVAQNLVWGTKASKTLFGHRDVSGDV
                     NINLTPEFASRLGSAFGSILKDDSGIIVSSDGSNSAEIMKESLISGVLSTGVGVISIE
                     NAAVPIMRFAVRFYKAEGGVHIWKDYSDKNKVHLEFSGSKGISIDRSVERKIENSFIR
                     EDFKRCNADRIKDIISAGNFTDFYIQSGINMLKYTDRIRSSGIKLVAASISSQIAGLA
                     QMYLKNMGCSVERDSVWDRYKMPAKDNISAGAYYEHIAELTVKSGSCMGVVFSEDGES
                     FVLIDHKGRILDKDKQIALTSLIILKTGMDKRLIIPHTASRVIEEMAKCYSIEVVRTK
                     NTPAAIMREMFQQNTGMEESLFQYILNFDGIWGIGNLVDFIAGNNVTLYDLVNELPHF
                     HMEKNEVKCDWQHKGRVIREIIEENKNREIELFEGVRINNENGWVLILPDSERPVCNV
                     YAEGASEEYARELAAEFTDKIKKIVSAEDK"
     gene            8453..16885
                     /locus_tag="PKI50_02605"
     CDS             8453..16885
                     /locus_tag="PKI50_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNT02638.1"
                     /translation="MNLVANYFYMAAFVLAAVLIYLLSRLLYFIDRDEPEVYDAILNA
                     EELEKHALELARKHVVGKRAKHYFSLEGRMNKNFRFITSVYKDLNEYANERSSVTPAA
                     EWLLDNFYIIEEQVKEIRQNLSRKYYYQLPRLKSGFLKGYPRVYAIALELVSHTDGRF
                     DDKQLVGFVSAYQSQSLLSNGELWAIPIMIRMALIEHVRHVCESISLSQQQWHKAEKL
                     AEQILSYKDRKPEELLAIAKESMRQLENISPSYGEHLLNMLKKHGIEIAPIIHYIDER
                     LSEQHTTSESITQLEHKEQAARQVSMGNSITSLRFISTLDWKVIFEELSQVEQILRQD
                     PAGIYQKMDFASRDKYRHELEILSKKLKVSEIKVARKLIECSVEDNRHIGYYLFREQK
                     EQLLDRIARKGMFIYSPAGVYFFSIITLTAFLVFLMLNYSYREGAAGFWTQLVIALAV
                     LIPASDVSVSAINWILVHIKPPAFLPKLELKDGIPEDAAAIVVMPTLITKGSSAVKLL
                     LKLEEYYLANREGNLYFALLGDYKDSDGEKSEGDQEIILAASEKVRELNRKYGSDEDK
                     FFFFHRRRVFSKTQNRWMGWERKRGALYEFNELLLGGKKASFEHIEGNISALKDVKYV
                     ITIDSDTKLSIETAKKLIGTITHPLNKAVVDEGRGVVKEGYGLLQPRISVDIVSANTS
                     RFARVFAGQGGIDIYTNAVSDIYQDYFGEGIFTGKGIYELAVFQGILRNMIPENTVLS
                     HDLIEGAHVRTGLVTDIELIDGYPSKYSASSMRLHRWVRGDWQLLQWLFSKVRSAKGE
                     IVRNPLSPINRWKILDNMRRSLVPPNLVLLFFLSLWLMPGNALVWLLLTLIAAVFPLF
                     VSFAEVVMTKYDRRMNEGSARGELGTLKRPFLQSLLALAFLPHQAQLMLDAIVRTLVR
                     ALITRKNLLEWVTAADMEHALKNDMASYWKRMWLCPASGAVLLLLSMFRSPEAIIAVL
                     PVALLWIAAPATAYWVSEPLAEKKNELSKKEIMELRILARKTWSFFEDFVSALDNYLP
                     PDNYQEDPPNGVAHRTSPTNIGLLLASIISARDLGYISTWEMLERLDNTITTIEKLKK
                     WRGHLYNWYDTVTLEVLRPNYISTVDSGNFIGYLMTLRQGLLEYKNKPSIDSGMVLGL
                     KDTSELVKLEDEQLSLPDEIFDKALEGDMLEQQGFIELLTKIIGKSDLKRFNWGIKLH
                     KSAITFLKEINSGIAQENNEELKIKSEGLIERIDNLIEETSFLPLFDNKRKLFSIGYN
                     EEEEQLTKSYYDLLASEARQASLISIARGEVKKEHWFMLNRTLTEVDGFKGLLSWTGT
                     MFEYLMPLLIMKDYSDTLLHETYDFVVRCQKDYGNKRKVPWGVSESGYYSFDFRLSYQ
                     YKAFGIPSLGLKRGLINDTVIAPYATVLALMVNPKESFKNILTLKEEGLDSPYGFYEA
                     IDYTPERLKRGKRSSIVRSYMAHHQGMSFMALNNVINNYILQERFHREPVIKAAEILM
                     QEKIPSKVIFAKDYKEKIEPFEVVVKEDVEYTKVLGLNNNILPEVHMLSNGDYSVMVT
                     DNGSGFSKYRDLAVTRWREDSVFNYSGMFFYLQDIDSGSVWSNSLAPCSVLPNKYKVV
                     FSQDKISISRTDDDIDTNTEIIVSPEDDAEVRRLTLTNHSQQVRTLEVTSYLEVSIAH
                     QSADLAHPAFNNLFINTEYLHKYNTLLSVRRPREEKGTRMWAFHELTVQEEVLGGIQY
                     ETDRAKFIGRGNMPDKPAAVGGSHPLSNTVGPVLDPIMSLRCRLRLQPGQTVKLNYIT
                     GVSDSKEKIMLMAEKYHDCSSIDRAFELAWTRSQVEMRYLGLKKEEIEVFRNMMSQIL
                     YLSPLRRKQESSIKGNRKGQPGLWAYGISGDFPIVAVFISRAEELDLVKELIKAHEYW
                     RLKGLFVDVVIINEEESSYLRPLYNMLQDIISVSHARDMRDKAGGVTIKQGYSMPEED
                     KQLIAAAARIVLKGKYGPLSVQTKVRNGKNAFEYKIWKSKGKKHNKYDIKKLEIKNFN
                     GFGGFDKEGREYVIKLTEGMNTPVPWCNVISNQKFGFLITESGASTTWAENSRENRLT
                     TWSNDPVIDPSGEIIYMRDEETGEIWSVTPHPIRTKTPYIVRHGFGYSVFEHNWNGIE
                     HELTEFVPVSDTVKLCILKLRNGSNTKMKLSAIYYARTVLGVYEQGTVQHVYTELHSN
                     GAILARNNYNTDFPGRIVFLDTNAKERYYTGDRREFLGNIGGLKHPEALRKDKLSNTL
                     GAGFDPCMCIQTVIELKPREQTEVVFMLGQGESLEAVSAYSERYRNVKRVYKALDEAK
                     AFWEDVLRKVTVLTPDENMNILLNGWLLYQTLSCRIWARTAFYQSGGAFGFRDQLQDA
                     MAVIDVLPDIARKQILLHASHQFIEGDVQHWWHPGSNKGIRTRFSDDLLWLPYVTVNY
                     INRTGDVQILEERVEFLEDEPLREGEDERYKIPCVSDVSASLYDHCVRAIERSLKFGH
                     HGIPLMGSGDWNDGMSTVGNKGKGESVWLGWFLYDILMNFSELCRMRRDLNKADRYIN
                     IAKEIAKAQEENAWDGNWYRRAYFDNGTALGSAENTECKIDSIAQSWSVISGAANLDR
                     AREAMQAVEQYLVKYDEGIVKLLTPPFDEGDLEPGYIKGYPPGVRENGGQYTHAATWV
                     IMAYSIMKNPDIAWKLFNMLNPINHANTMMEASRYKVEPYVMAADVYAVPPHTGRGGW
                     TWYTGASGWMYNVGMQHILGFRKKAGRIVMEPCIPRDWPEYNIRYRHNKNTIYNIIVR
                     NQNRSGNAKAEIILDGRKLEGNEFPLAEDGAEHTAEVILT"
     gene            16974..17231
                     /locus_tag="PKI50_02610"
     CDS             16974..17231
                     /locus_tag="PKI50_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HNT02639.1"
                     /translation="MSERFEDTMKFKVETDKKYEASDILLSVYSALKEKGYNPINQIV
                     GYILSGDPTYITSHNNARSLIKKLERDELLEELVRAYVDGK"
     gene            17259..19376
                     /locus_tag="PKI50_02615"
     CDS             17259..19376
                     /locus_tag="PKI50_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02640.1"
                     /translation="MNKSLIAFFSIAALSAAIIALQDVKAYEIFASGEEEYGKVVMIV
                     GDYLDIKDIGRMESLYRLAGNSRVALINNRQPGKADAAKSKLIIGSGKKLELDADMVS
                     EGRRESLTGSLVYTGINRIKDRNADSEYKKYIGYIGDAINKKGGTACYIGNADAEKPN
                     KSSMLIAADSSGEIDSGETEDAIIEDAAFPGGKRTDYDRLAELYKQYLPASSFIVIET
                     GDMERLEAFKGSMSEEAWQSCKQDVLERIDGFVKKIVSYGGFKTLVFISTYPSKSNAE
                     AGNKLTPIVVYEGREGVLYSNNTRRSGIILNTDIADYVLCKLGYLTECAVEELDKKAS
                     LNALRYMDMSILRTSVLRTPVLTYYAVSVIAVLGILFGAAVFLGKKAGHFWSGFGIIT
                     AYTMLSIPIAFLLIPASYTDWGSAEYSVLTAAASAVLSILLHLTLRNRLKVILAICLL
                     LQAGLAADILTGGELIKKSVLGYDPIIGARFYGIGNEYAGMFIGISLMVFGCVSELRG
                     RRCGKAAAAAYFSFCTLLLGLTSLGANFGGAVAGVFGYLLAYFLVFDIKFNRRNIFTG
                     ALILGSAAAALFIADSLGLARQSHMGALIKDTRANGVGVITSVISRKISMNLRLMRYT
                     IWTKVLLCIIGAISIMFYKPVKQMFYIFNRYKYLKYSWMSIAASAAAGFAVNDSGIVV
                     AATAMIFMAFTMLIMCIGEGKAV"
     gene            19379..20326
                     /locus_tag="PKI50_02620"
     CDS             19379..20326
                     /locus_tag="PKI50_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT02641.1"
                     /translation="MDYRILGSTGLRVSRLCFGSLTIGPLQAGLDIEEGASVIRAAFD
                     MGVNFIDTAELYRTYDYIRKAAEGRRKDIIISTKCYAYTREGAEKSLRKALDELNTDY
                     IDIFSLHEQESEHTLKGHQEAIEYFVRAKEKGYIRSIGISTHAIAAVRASLKYKDIEV
                     LHPILNKRGLGIIDGDVNGMLEAAAEAYKAGKGIYSMKPLGGGNMIKDSSQCFDFVLS
                     NDNLHSIAVGMQSYEEVASNVMIFEGKQVPEDIRLKLEKKKRRLLIDYWCSGCGSCSG
                     KCAQKAISVEKGKAVVDSSKCVLCGYCSAYCPDFCIKVV"
     gene            20422..20847
                     /gene="ruvX"
                     /locus_tag="PKI50_02625"
     CDS             20422..20847
                     /gene="ruvX"
                     /locus_tag="PKI50_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNT02642.1"
                     /translation="MRIMGLDLGDATIGVAVSDELGMIAHGITTIRRKSMYYDIDCLK
                     NIICEKDIKRIIVGLPKNMNNTIGPRGEKTIEFVRILEENFPDIEVEMWDERLTTCAA
                     ERTLLEADVRRKDRKKVIDKLAAVLILQCYLDNMRNDNP"
     gene            20924..21205
                     /locus_tag="PKI50_02630"
     CDS             20924..21205
                     /locus_tag="PKI50_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNT02643.1"
                     /translation="MSDEMDIILLYDENDNETEFEVVATLEVDENEYAILLPRDDTRD
                     PDAEEVDEAYVLRIEQDENGEDILIGIDDEEELNTVIEAYEELVREESN"
     gene            21391..22320
                     /locus_tag="PKI50_02635"
     CDS             21391..22320
                     /locus_tag="PKI50_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT02644.1"
                     /translation="MRPELIEFNDGIPVRAFVRSGNQYPYHWHDALEIIQVLKGSVDI
                     GMGDDDLVLREGDIAVTNINEIHRMTGDGEDEILFLHIDSGFCRSVLPNHYIFIYCCS
                     PYHEAQAPEKYGVLKEYIARLVGMLGSSPNRIGQEALRKPLMEMLSYTAYSFDFLRWG
                     FGTEPFDEKRVARLRQMAQNATGDSEVKKGLTILAAELGVSLQHLSSDIKEKFRVPFQ
                     ELLCYSRCERAARLLLGTDMRIVEIALDCGFSDVKYLIKYFRRFFHATPSGFRKLHRA
                     DSAALARQTQYSDMSLGHAVRLLSAETDGDHIE"
     gene            22520..27139
                     /locus_tag="PKI50_02640"
     CDS             22520..27139
                     /locus_tag="PKI50_02640"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT02645.1"
                     /translation="MKHLFRKTTALFLTLCMIVGMTGFPITAYAEGLDSGDGGAADKA
                     APKIGFSVTDSVYGVTELKEEKLSMLFSPMSAVEWVGIGDENSPYQVSSAEHLADIPN
                     QGMGAHYIQTADINLTGINWTPIGDNSSFDSTTQFTGTYDGNNKTISNLTINNTELYY
                     VGLFGYAGSGGTLENIILENVSINSSRDSAFVGGLTGQNCGGTIKDSCVSGSITATGD
                     SARAGGLIGDSYLGGTVENSSSSCAVTGGEGVSNTIGGLVGRQQGGSIRNSYSTGNVT
                     GTYYVGGLVGYLLGSGTIEHGYSTGNVTGTPSDGTIGGLVGTVNNGTITNSCSTGNVN
                     GTNNASNYGIGGLVGYFYGSGGIANSYSTGSVSLLNSGSDTNQGGFIGLIQSTSATIA
                     YCYSTGDVTPGDGSVGGFIGLVRDNPTVTSSYYLDTAAASSARGTALSATALKQRESF
                     VDWDFDTDWKIGEGVSSPTLRWQPWTTDEKITAAHDALDWDAIRGANTLESDVTADLV
                     LNASGAQGASISWSAAPAGFINTETGEVTTPLYSQGDQTVTLTAAISHADGGTPQTKD
                     FTLTVKNVPVTWVGSGDADSPYQVSSAAHLDEIRIKGLGAHYIQTANIDLTSINWTPI
                     GDNVDQFTGAYNGNGYTIANLTINDDGQNYVGLFGSVGTGGTLENIRLTGVNISSITW
                     GAYVGGVTGVNQGTVTNSSSEGSVSGGDSANVGGLIGNGGGVITYNYSAANVTGGNNA
                     NVGGLLGRSYVSTSDNYSTGTITGGDNARVGGLIGDVDDGTVTNSYSTGSASGGTGAS
                     LGGLIGGGNATVNDSYYLDTAGTSAGGDPLTAVQMKQWDSFEGWNFNTIWKIGQNISY
                     PTLQWQTWTADEEITAAVAALAWTDIQGTNSAENDVTSALVLIISGGRDTVISWSADP
                     TGYINTSNGTLSKPYTDEVIVTLTATVSKGSGIPQTKPFSLTIKPLTPDEAITLDKAA
                     LTWAVIKGTNSTEDHVTADLELPDTGTSGTTAITWSADPVGCIDISDGKVTFPDDGNK
                     AVTLTAAITRDGGTAQTKDFTLTIIAHYPVTFTAGTGGSITNGTSGSYAAGTVIEISA
                     APDSGYQFDKWITGSGGSFGNAASASTTFTVPANAVTVTATFTRSSGDRDDRGNKNAP
                     KAAEPEYKAEVKNKDGEEAVLTVTVDKNGGTASVDAGLQSFDIDGSVITIPTVSDIEA
                     YSVSIPMTSLSTADAQGTLTVNTDKGSITIPSNMLTGIPGISGSKAQISIGQGDKNIL
                     PDAVKAAVGDRPIIRLTVSIDGKQTDWSNPNTPVTVCIPYTPAAAELANHESIVVWYI
                     DGSGNAVSVPNGRYDPATGTVTFNTTHFSHYAIVYVHKTFSDLCSAEWARKPVEVMAS
                     KGIINGTGNDTFSPAASITRADYLVLLVKTLGLAAEFDSNFDDVDKSAYYYDAIGIAK
                     KLGIVAGSGNNRFNPRESISRQDMMVMTARALERFKGLKVLDTNGVPEKFADKEDIAE
                     YAVNSMSALITEGLIAGTGNKLNPCAYATRAEAAVFLYNIYSNYP"
     gene            27312..27530
                     /locus_tag="PKI50_02645"
     CDS             27312..27530
                     /locus_tag="PKI50_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNT02646.1"
                     /translation="MTLDKVARGNKVRITKINNRDMKTQAIRIGLYEGADFLCSERLA
                     GGPIILRSRMQEVAIGRRLAEGIEVEKI"
     gene            27559..29400
                     /gene="feoB"
                     /locus_tag="PKI50_02650"
     CDS             27559..29400
                     /gene="feoB"
                     /locus_tag="PKI50_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNT02647.1"
                     /translation="MSGCNNCNKNCIKDIKDKKLVLAGNPNSGKSVFFNHLTGIYVDV
                     SNFPGTTVDISTGKYKGYTVADTPGVYGVSSFNEEETVARDVILEADIILNVVDSVHP
                     GRDLFLTKQLIDMGKPVLVALNMIDELQKNGMDIDVKLLEELLGVPVFPTSAVKNIGV
                     TDVLMSLEQARQGNRDPGLTEMMKQFGDTCSEAEALMILEDDEVVAARYGIETSGLRD
                     EIYMERRRAVEEIVNKVLKETSKGASFGILLGRWMLRPVTGIPMLLLTLYAMYKIIGI
                     FVAGTVVGFTEELIMEGMYKPFIISVLSLFISEGSFIGNMLIGEFGILTMVPVYIIGL
                     LLPLIIGFYVLMAVLEDSGYLPRIAALADRMLISLGLNGKAIIPIILGFGCVTMATMT
                     TRILGTKREKIIATVLLGLAIPCSAQLGVIMGLVSGLDVFYIITYITVITSIFVLAGT
                     VLNKLMPGESSHLLIDLPTIRLPMPGNVVKKTFQKTVMFLKEAVPLFIYGALLISVLE
                     QTGALSYIQQWMTPITEGLLKLPPETATAFIMGIIRRDFGAAGLTSIAMTQQQLLVSL
                     IVMTLFVPCIAAIMVIFKERGTRDALAIWLGSFAAAFITGGIVAWLI"
     gene            29388..29573
                     /locus_tag="PKI50_02655"
     CDS             29388..29573
                     /locus_tag="PKI50_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02648.1"
                     /translation="MVDMKNGLKCGKCGYTLKEQSKCPRCGTVAQCSLSCPGNCLKCF
                     AEAAKANKQKLVDKNAE"
     gene            29685..30119
                     /locus_tag="PKI50_02660"
     CDS             29685..30119
                     /locus_tag="PKI50_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNT02649.1"
                     /translation="MREFEYLREKLKEKGFKLTPQRRAILDIILETEGKHLSAEEIYD
                     LVKVKCPDIGLATVYRTMQMFEEVGLVYKHNFDEVRSRYEINHHNEYHQHHHLICVGC
                     SKVIEVEEDLLEQLESGIEKKYNFKISNHNVKFFGYCEKCRK"
     gene            30297..31985
                     /locus_tag="PKI50_02665"
     CDS             30297..31985
                     /locus_tag="PKI50_02665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNT02650.1"
                     /translation="MKGLIKKVAKSKAKLKLIPLGGLNEIGKNMTAVEYKDTIVIIDC
                     GLMFPDEEMLGIDLVIPDVTYLVKNKEKVKAILLTHGHEDHIGALPYVLPQINVPVYG
                     TKLTLGLVENKLKEFGLLSLVERIIVKPKDMVKFGELEVEFIKTSHSIADSVALAIHT
                     PVGIIVHSGDFKIDYQPIDGELIDLHRFAELGKQGVLVLMGDSTNVERPGYTMSESTV
                     GATFMELFRDAGGRVILATFASNIHRIQQAINAAAMFNRKVAVVGRSMVNVIGVATEL
                     GYINIPEGVMIEVDHIDKYPNDRIMILTTGSQGEPLSALARMSTAEHRKVTIIPGDRV
                     VISANPIPGNEKLVSRVINQLFKKGADVIYESLVDIHVSGHAYQEELKLLQALIKPKY
                     FVPVHGEYRHLTQHAQLAEKMGVNKENIFIADIGTVLEFTKDSGRTAGTVVAGKVLVD
                     GLGVGDVGNIVLRDRKHLSQDGLIVVVVTISKETGQVVAGPDIISRGFVYVRESEDLM
                     EEARGVIKDALAKCEEKQITEWGTIKSEVRDSLREFIYERTKRNPMILPIIMEI"
     gene            31992..32912
                     /locus_tag="PKI50_02670"
     CDS             31992..32912
                     /locus_tag="PKI50_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNT02651.1"
                     /translation="MDKICIVVNPVSANKTTAREWPLFEKVLSDAGFEFEAVLTEYPG
                     HAAELTRKALKLGYNTIMSVGGDGTMNEVVNGFFENDRLIDENSRLVVFSRGTGCDFV
                     RSLGINRNIEDLLAILKRNREKQIDVGRLSLVRTEGNMPARYFLNVADTGIGAETANR
                     VNMHSKRLSGFLSFMLGTLSTVVLYKNQNFEVVIDDKIHLNGIMNSVIVANGKFFGGG
                     MMVAPDAVLDDGVFDIIILGNLSKYDIIKSFPLIYKGKHMNHPKLKYCRGSKVKVTSG
                     GKGLIEADGEIPGRADAVFELMPKALKILI"
     gene            32940..34760
                     /gene="typA"
                     /locus_tag="PKI50_02675"
     CDS             32940..34760
                     /gene="typA"
                     /locus_tag="PKI50_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNT02652.1"
                     /translation="MEKTIREDIRNVAIIAHVDHGKTTLVDGLLRQSGTFRANENVME
                     RVMDSNDLERERGITILAKNTAVIYNNIKINIVDTPGHADFGGEVERVLKMVNSVLLI
                     VDAAEGPMPQTRFVLKKALELQLKPIVVINKIDRPDSRIEEVIDEIINLFIELGADDN
                     QLEFPIVYVSAKQGVAKLDIKDESQDLRPLFDTIISYVEPPKGDAEGPFQMLVTTIEY
                     DEYVGRIAIGRIERGRIISGMSAALCKRDGVLQSVKLSRIYSFMGLKRKEVEEAFLGD
                     IIAVSGIDDINIGETICDIQNPEAIPFVNIDEPTISMTFSVNNSPFAGREGDYVTSRH
                     LRARLFKELETNVAMRLTETDSPDTFEVAGRGELHLSILIETMRRQGYEFQVSKPNVI
                     MRNINGAACEPIEHLIIEIPEEFMGVVMDRLGPRKAEMMNMGNSSDGYLRLEFKIPAR
                     GLIGYRSQFMTDTKGNGTMHHVFYGYEEYKGEIPDRPRGSLIAFENGETTAYGLYNAQ
                     ERGYLFLGPGVQVYEGMIVGENAKSDDIEVNVCKKKHVSNMRASGSDEALRLTPHKEM
                     SLEECLEFIAADELVEVTPKSIRLRKRILDSSLRAKARNK"
     gene            34939..35961
                     /gene="mltG"
                     /locus_tag="PKI50_02680"
     CDS             34939..35961
                     /gene="mltG"
                     /locus_tag="PKI50_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNT02653.1"
                     /translation="MKAKLKFNIFRFLVFCSLLALVLAYVYYNNGMEAVSGEGSDFKE
                     IVIPKGSTIRYISKILKEEDLIKDSLIFELYCKINEKADKIKAGRYSISSSMKVSEIV
                     DVLVSGKALIDTVKFTIPEGYNLAQIAERISSLGVVSPESIQAALKAEGYEYEFIEQI
                     PDRENKLEGYLFPDTYEIYRDTTAEAIIKKLLGRFDDIFTEKFRSRAEELNMSIDQVV
                     TLASIIEREAKLDSERKTISGVFHNRLKKKMLLQSCATVQYLLKEPKEELLYEDLEID
                     SPYNTYKYAGLPPGPIASPGLAAIEAALYPENTDFLYFFALEDGSHVFTKTYNEHINT
                     QNKYRK"
     gene            36220..36885
                     /locus_tag="PKI50_02685"
     CDS             36220..36885
                     /locus_tag="PKI50_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNT02654.1"
                     /translation="MANISHDYIDSFLLGMIPGRSGILKEMEEYARENHVPIITPNIA
                     SLLAVLIKTAKVKSILEIGTAIGYSAIHMGLCAGKDFSITTIERNEEYAALAADFIRK
                     AGMGDNIRIITGAAEEMLKDIPGSFDMIFVDAAKGQYMDFMKKSIGKLRIGGLYVCDN
                     VLFRGMVAERSLLIRRKITIVKRLKKFLSFITSCESLETAVIPMGDGISVCCKLKEVE
                     FIE"
     gene            36878..38113
                     /locus_tag="PKI50_02690"
     CDS             36878..38113
                     /locus_tag="PKI50_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNT02655.1"
                     /translation="MNKPELLAPAGNLEKLKMAIIYGADAVYIGGEKYGLRAAAGNFS
                     LDEIKEGAAFAHDNCRKVYVTVNIIPHNEDLEGLEEYLTSLGDAGADALIVSDPSVIM
                     TAREVLPDMQLHLSTQASNTNYMSARFWHSQGIKRIVLARELSFEEIRETIEKSPKSL
                     EYEVFVHGAMCISYSGRCLLSNYMTGRDANRGECAQPCRYKYYLVEEKRPGQYMPVDE
                     DERGTYIFNSRDLCMIEFIPELAESGVKSLKIEGRMKSSYYVASVVRAYRMALDSYFS
                     NPGNYEFRKEWLEELSKASHREFGTGFFFGKPDSRGQIYESGAYVRDYAFVGLVLDYD
                     SKTGIATVEQRNKMLVGDEIEVIGPRREMFTQKIEKMWNDEGVEILSAPHPQQIVTIK
                     MERPVEKHDILRRERKDGE"
     gene            38106..38756
                     /gene="udk"
                     /locus_tag="PKI50_02695"
     CDS             38106..38756
                     /gene="udk"
                     /locus_tag="PKI50_02695"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010173838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNT02656.1"
                     /translation="MSEEKHRKPLVIGIAGGTGSGKSTVSKAITDSIPGKKVVIIEQD
                     CYYKDQSHLPPEERVNVNYDHPLAFDNELLIAHMLKLISGQAIDKPQYDFTVHNRKKH
                     TLRVEPKDVILLEGILILDSPRLRELMDIKIFVDTDADVRVIRRIVRDMRERGRSLES
                     VITQYLNVVKPAHNEFCEPTKRYADIIIPEGGYNKVAIDIMVTKIKSIIDGGCDSN"
     gene            38809..40584
                     /locus_tag="PKI50_02700"
     CDS             38809..40584
                     /locus_tag="PKI50_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNT02657.1"
                     /translation="MAGIMKFLRKKAVPECRKEEENRSMELLTLPGTRLYRKNNYILI
                     HKKRLQVIGLLFIAACGLLLCRLFYIQVYMGKHYSERAVRQRMVHIPISSNRGIIYDR
                     NMIPITDREVKKVVIAYPDYVYDKKTAVETISKACGIKMEDVEKKMGSADTAEFICET
                     EQNEYLSLIESGRIRGVIAVEKRLRYADDNIARHVVGYIGKTDKTGQMGIEKSMNSYL
                     ESRGSDSIAAVVDSGKNIIRGLGFRKVEAAGDGVSYSLKLTLDYHIQNIIETAMEKND
                     VEGSIVVMDVKNGDILGMASKPDFDQGNVDKYLKSSGNELINKSIWQFDLGSIFKTVV
                     AAAAIEGGYVNPEEKYKCRGYADVGSSRIKCSTHKTHEDIEINMKEAFALSCNSTFIQ
                     IGTATGAENILKMAGRLGLGQKLCFTIPEEKAGYIPEAFEDGIGNISIGQGKIQVTPL
                     QVTCMMAAIANNGIRNDPQIIDSLITEKGLTVKKLERSRPQIVLNPITSKLLKDMLHE
                     VTVTGTGKQANMDEYGGCCGKTSSAQTGIKEGEVVHGWFAGFIPYEKPKYAITVFIYN
                     GQSGGGAAAPLFKEAASKILTAVKR"
     gene            40725..41432
                     /gene="sigK"
                     /locus_tag="PKI50_02705"
     CDS             40725..41432
                     /gene="sigK"
                     /locus_tag="PKI50_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HNT02658.1"
                     /translation="MLLFLASLIVFTIKDLCLLFGYIQNVNSFPQPLTTEEEQYYLEE
                     YKRGNEDAKNILVERNLRLVAHIVKKYVNTGREIDDLISIGTIGLIKAIITFDESKGT
                     RLATYAARCIENEILMTIRASKKTKTEVSLHDPIGIDKEGNEISLIDILGSDSDIILD
                     EVELKIQIKKLYQRMDTALKKRERLVIELRYGLLNGISKTQREIADMLGISRSYVSRI
                     ENRAIKKLHKELYNENG"
     gene            41511..42017
                     /locus_tag="PKI50_02710"
     CDS             41511..42017
                     /locus_tag="PKI50_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HNT02659.1"
                     /translation="MKNRLKPDIYLNSVYELDTASLKEHGINAIIVDIDNTLVSWNTR
                     EPDEKANEFMCRLGSEGFKICILSNNSRKRVEEFNKCLNLPAIHKATKPSKFAFRRAM
                     KLMKSKPADTAVIGDQLFTDVLGGNRLGLFTILVSPISNKEFIWTRIVRKLEKHVLRK
                     YRQTNNMQ"
     gene            42178..42816
                     /locus_tag="PKI50_02715"
     CDS             42178..42816
                     /locus_tag="PKI50_02715"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNT02660.1"
                     /translation="MHGLTVISSSAFTAASVLLVKLLERKVGAGKGDKIGLSFAAPVF
                     ILFLSVNLYMINKNAEGSYLILNVLLLTAVVSSYQDIKYREIEDEIHIAALGAGLVGL
                     LIKGFQPADAFLGLMAGGGTLLLIAILTKGGMGGADIKLNAVYGAILGLKLSMLSLVI
                     AFAAGALASLLLILFGIKKRKDTIPFSPFLSIGALTAFVFGKSIMGIYLGFF"
     gene            42842..43114
                     /locus_tag="PKI50_02720"
     CDS             42842..43114
                     /locus_tag="PKI50_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HNT02661.1"
                     /translation="MYKLKNYMEDLVIKRTDEILKSIDVCKCDKCKLDIIALALNSLP
                     SKYVVSAKGELYAKVSELEQQFDIDVGTAIIKAAFLVNENPRHNEE"
     gene            43217..44098
                     /locus_tag="PKI50_02725"
     CDS             43217..44098
                     /locus_tag="PKI50_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT02662.1"
                     /translation="MIHFGPSGNSDSFYEQGYKSSLDMPAWLRDMGLDAYEYSCARGV
                     RIKPDMAERLGKAARENGITLSVHAPYYINLASEEPEAVKKSINYIIDTSITAELMGA
                     MRVVLHPGSLGKLTRNKAFENIRSNLFRVLEIINKKGIKVSLCPETLGKRNQMGTLDE
                     ILELCLLDELLIPTVDFAHLHALGSGALRKREDYEKVFDRIFTVLGEKRGRLMHMHYS
                     RVEYTEKGGEKKHWSYDDVEYGPEFEPLAECLIKFGISGTIISESRGTMAEDALKIKK
                     IYGRLSKENLYAMEKLC"
     gene            44166..44723
                     /gene="efp"
                     /locus_tag="PKI50_02730"
     CDS             44166..44723
                     /gene="efp"
                     /locus_tag="PKI50_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNT02663.1"
                     /translation="MISASEFRKGLTFEMDGQIYTIVDFQHVKPGKGAAFVRTKIKNI
                     ITGGVTERTFNPTDKYPKAHIERKSMQYLYEDGGLYYFMDNETYEQIPLNHEQVEDAI
                     MYLKPNDTANIKFYKGEAFSVEPENFVEMEVTQTEPGFRGDTATGATKPAIVETGATV
                     YVPLFINTGDRIRIDTRTGEYMERV"
     gene            44790..45209
                     /locus_tag="PKI50_02735"
     CDS             44790..45209
                     /locus_tag="PKI50_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02664.1"
                     /translation="MEYLYERVAYLKGLAEGMKVDESTDQGKLITKIIDVLSDFADAV
                     NELNESQNELDEYVEAIDEDLSALEDDLCYDEDDEEEFDDEEDYDYIEVECPHCKEFV
                     YLDEDLVESGEQVICPNCHEPIEFEYECGCGCDHDQE"
     gene            45347..46354
                     /gene="spoIIIAA"
                     /locus_tag="PKI50_02740"
     CDS             45347..46354
                     /gene="spoIIIAA"
                     /locus_tag="PKI50_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HNT02665.1"
                     /translation="MKNDIKILEVLEYMPKHIGAMIAKLPEKILMDAEEIRLRLAKPL
                     CVAGSNGELFISADGYAVSEAEEAYLVSEEDLKRALQLTCNFSLYSVEEELRNGFITV
                     SGGHRVGVCGRAVVDSGKVKTMKDISYMNFRVAKQVLGASDKVSGYLVQPPDSIFSTL
                     IISPPQCGKTTLLRDLVRKLSSGSRDIPFKGMKISLVDERSEIAACSMGIPRNDVGIR
                     TDVLDGCPKAEGIIMVIRSMSPEIVAADEIGRAEDADAITAAVNAGVKVITTIHGSNI
                     TDFLNKQDLKRIQKGVFERYIVLSRKKGVGTLETVLDGEFNVLYERSKSICKWGDRVD
                     A"
     gene            46347..46865
                     /gene="spoIIIAB"
                     /locus_tag="PKI50_02745"
     CDS             46347..46865
                     /gene="spoIIIAB"
                     /locus_tag="PKI50_02745"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein SpoIIIAB"
                     /protein_id="HNT02666.1"
                     /translation="MLKIIGGLLVISASGLLGMVYSYKLSLRYRELANLRRLMQMLET
                     EVTYGATPLPAALFSVSKKAEGPIGSFFSCISRCLSDRSFYTVREAWNDSAESILTQS
                     SLKMADIDLIKSFGSILGCSDREDQKKHFQLFYLQLSHQEAAATEEIKRSAKMYRSLG
                     FLLGIAVFVVLA"
     gene            46889..47083
                     /gene="spoIIIAC"
                     /locus_tag="PKI50_02750"
     CDS             46889..47083
                     /gene="spoIIIAC"
                     /locus_tag="PKI50_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HNT02667.1"
                     /translation="MDVDLIFKIAAIGIVTAVLNRVLNQSGREEMAMMTTLAGVVVVL
                     MMVISLISNLFNSVRTMFHL"
     gene            47093..47479
                     /gene="spoIIIAD"
                     /locus_tag="PKI50_02755"
     CDS             47093..47479
                     /gene="spoIIIAD"
                     /locus_tag="PKI50_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HNT02668.1"
                     /translation="MDIMKIVMVGIIAALLAVVLKEEKPEMAFAISIVTGLVIFVFVI
                     TKLNSVMTVLKHFAAKANIDVLYFTTILKVIAIAYITEFGAQICRDAGEGAIAAKVEF
                     AGKVLIMVIAIPILAALMDIMIKIIP"
     gene            47491..48651
                     /gene="spoIIIAE"
                     /locus_tag="PKI50_02760"
     CDS             47491..48651
                     /gene="spoIIIAE"
                     /locus_tag="PKI50_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HNT02669.1"
                     /translation="MGKGIVFFMIIILLSLCAANCDDTQDILQSQLDNLDISDIEKFV
                     DDINRESEGMLPEIDVREMVLGLFKGNTQNSIRDMGKGIASLFFKEVISNFSLMGKLL
                     ILAIFCAILQNLHNAFEEDTIGRLAYGMCYILIIILAVKSFNDAMLIGVDTINKMTGF
                     MQALLPTLLALLASVGGISSSAVFQPLMFTCIALITTFIKVFVMPVILFSAVISILNN
                     LSDSVNISKLASLLKQIAVGALGLVLTAFTGVLTVQGIASASIDGATIRTAKFAVDNF
                     IPIVGNFLSEAFDAVVSCSMILKNGIGIAGFIILLLICSFPLIKMLSVVLIYKVTSAL
                     IQPILDNQIVQCLNDMGNAMLVLLTCVLAVAVMFFMALTVIMGVGNMAVMVR"
     gene            48669..49304
                     /gene="spoIIIAF"
                     /locus_tag="PKI50_02765"
     CDS             48669..49304
                     /gene="spoIIIAF"
                     /locus_tag="PKI50_02765"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HNT02670.1"
                     /translation="MADFLREWIINITVIIIFIMFLDTIIPNSSMKRYINVIVGLMII
                     VVVIKPFVLIKDYADSFNMKFLETSSYIEQGGSAADSTEISNFQRQMAVEIFENNLKK
                     KVGRIVDNSTDSDYEEVSIDLRLEKDFESKDFGEIKSIEVIISKRPKEVIEVDRIKIA
                     ADEDAEVNKNVINKDKVEYNLNNSRIRSEIRDGISKALGISESIIGVEVRQ"
     gene            49326..49949
                     /gene="spoIIIAG"
                     /locus_tag="PKI50_02770"
     CDS             49326..49949
                     /gene="spoIIIAG"
                     /locus_tag="PKI50_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HNT02671.1"
                     /translation="MKLHEWLMDKLRKVNFQKLNFSKKTTANLIIVFCLGLALILIAD
                     FYRDIRRDRASEEGIFSEITQADTGTSDNNASDYVRRLEADLSSILSKIQDAGKVSVM
                     ITLESGAEIIPAKDESTSDKVTNEKDTSGGTRIINEKTTDDKVVLTAAQGGNSKPLII
                     KEINPEVKGVIVVAEGAKDSKVKLKISQAVQTVLDIPAYRVTVYERN"
     gene            49980..50564
                     /locus_tag="PKI50_02775"
     CDS             49980..50564
                     /locus_tag="PKI50_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HNT02672.1"
                     /translation="MQVKKKNLIICSMIILLFVVGYAYNSFTSERLTDANLDLDAQVI
                     GTDEMGMPAETAGTEEAAGPIVIEDSGSEALETTAASFFSEYRIERDKNRSKEVEMWQ
                     DIINSDKAEENFKNMAQQEIVKIVSLTDKEMIIENLIISRGFNDALVFLTDDSATVIV
                     EAKELTSSNIAQIQDIVVRKTKLDPKNIKIMKKN"
     gene            50719..51114
                     /locus_tag="PKI50_02780"
     CDS             50719..51114
                     /locus_tag="PKI50_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNT02673.1"
                     /translation="MEKEIKLDKYGNVKIADDVVAIIAEIAAREVQGVYSMSGGIADS
                     ITEILGKKNPSKGVKVEVGEKEAAIDLYIIVDYGVRIPDVSWQIQENVKKAVETMTGL
                     TVVEVNIHIQGINMDKESKEEENPEGKSK"
     gene            51204..51662
                     /gene="nusB"
                     /locus_tag="PKI50_02785"
     CDS             51204..51662
                     /gene="nusB"
                     /locus_tag="PKI50_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT02674.1"
                     /translation="MSRRLAREFAIQFLYSTDFNRNENTDEMLEDFYEIKKEQGKDGQ
                     QDSMKEADMKFAEEIIKGTIGKLQEIDQFIQNNTTGWTKERIAKVDLAILRLALYEIL
                     FRDDIPDSVSINEAIELAKKYSTDESGSFVNGVLGKIIREADIKVYGTNE"
     gene            51655..52866
                     /gene="xseA"
                     /locus_tag="PKI50_02790"
     CDS             51655..52866
                     /gene="xseA"
                     /locus_tag="PKI50_02790"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNT02675.1"
                     /translation="MNNERIFTVTEVNNYIKHLIQNDVLLSHLHVKGEISNFKHHSSG
                     HIYFTLKDADSRIRCVMFKGNASKLKFMPEDGCAVILRGYFSIYERDGQYQFYTEDMI
                     PEGTGSLYKAYEQLKKKLAAMGYFDEARKKKIPFMPRAVGVVTSPTGAAVRDIISIIR
                     RRCPCIDVYLYPVQVQGAGAENEIAEGIRYFNSKGNVDVMIIGRGGGSIEELWAFNEE
                     VVAKAVYESDIPVISAVGHETDYTISDFTADLRAATPSAAAELAVPDMQSLLGKLSQY
                     QYVLNSAIANFIKDNRSRVERISGDMVFRQLERKVLNMRQTLDSLDKYLDYNMLNIMK
                     GRRNEIDKTIEKLQLLNPEAAMGRGYGIIMRKSDGKLLTSVKDMKLNEELYIKLKDGK
                     AEVRVKGLEST"
     gene            52916..53152
                     /gene="xseB"
                     /locus_tag="PKI50_02795"
     CDS             52916..53152
                     /gene="xseB"
                     /locus_tag="PKI50_02795"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNT02676.1"
                     /translation="MNENFSFEQALKRLEEIVETLEAGNISLEESIKIYQEGIALSKL
                     CSGKLEEAEGKVMAIMNRGSEQMEEFSISQIKEA"
     gene            53155..54036
                     /locus_tag="PKI50_02800"
     CDS             53155..54036
                     /locus_tag="PKI50_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT02677.1"
                     /translation="MDLKEYLANKRHLVEQKLKSMIPADNSIPVITEAMRYSLLAGGK
                     RLRPILAIMSCELFGGQEEEVLPFACCIEMIHTYSLIHDDLPAMDNDDLRRGRPTSHK
                     VFGEGFAVLAGDALLNSAFETMTAILMDNPKPEYIRAMNIICRASGISGMIGGQCIDL
                     YYENRSIDIETLKSMHRKKTGALIKASLAAGAVIARADEGDIKNIMEFGDAIGLAYQV
                     EDDILDVSGSEEKLGKSINKDAEFHKSNFISFYGLDKSISIAAELIGEAKKKLERYGD
                     KSCYLIELSDYIIDRDN"
     gene            54094..54540
                     /locus_tag="PKI50_02805"
     CDS             54094..54540
                     /locus_tag="PKI50_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNT02678.1"
                     /translation="MNFFVKVADNKALLACITAWLIAQILKIIFTFYSSKKLDLTRLV
                     GSGGMPSSHTAFVMALSTSIGRMYGWDSPIFAVSLCFAFVVMYDAAGVRRAAGNQARI
                     LNMIIEDLAHNKPITDEKLKELIGHTPKEVMAGALLGIVVANIMVR"
     gene            54576..56426
                     /gene="dxs"
                     /locus_tag="PKI50_02810"
     CDS             54576..56426
                     /gene="dxs"
                     /locus_tag="PKI50_02810"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNT02679.1"
                     /translation="MLDSINSPADLKKLSYQELEVLSKELRDYVIKVVSENGGHLASN
                     LGVVELTLALHRVFDTPGDKIVWDVGHQSYIHKIITGRRESFKTLRKINGISGFPKRE
                     ESVYDSFETGHSSTSISAALGMAKARDLAGENYSVAAVIGDGALTGGMAFEALNHAGD
                     FPTNLIVILNDNKMSISHNIGGLSNYLSKLRTEPLYFKMKGRIEAFLHRIPVIGGSMY
                     RGAERLRDWMKYLLVPGVIFEELGFKYFGPINGHDIRKLEDVIKRAKSYNGYPVLIHV
                     VTTKGKGYSIAENKPDIYHGISPFFIENGELKSKNSRKTYSEIFGDTMLKLADKDNKI
                     VAVSAAMAEGTGLSAFASKYKDRFFDVGIAEQHAVTFCAGLASEGFRPVFAVYSTFLQ
                     RAYDQIIHDVCMQKLNVVFAIDRAGIVGSDGETHQGVFDITYLRHIPNIRIMSPKDGN
                     ELSEMLKMALSLEGPTAIRYPRGKAKKFEFAHAPLELGKSEVLTEGEDGVIISEGNMV
                     SIALEVCEKLKKAGRNFTLVNARFIKPLDEELLVGLSKKHKLIYTLEDNVVSGGFGSS
                     ILELLSRLRADVDVRIIGFDDSFIPHGEKEELYSLKKIDSESVFNAIMKE"
     gene            56433..57245
                     /locus_tag="PKI50_02815"
     CDS             56433..57245
                     /locus_tag="PKI50_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNT02680.1"
                     /translation="MAEKERLDVLIHKCGMSESREKASSLIMSGIVYVNGIKVDKPGT
                     KLATDSNIEIRGNALPYVSRGGLKLEKALCEFGINLKGKVAIDIGASTGGFTDCMLQK
                     GALKVYAIDVGYGQLAWKLRTDSRVVCMERTNIRYVAPEDISESADFSSIDVSFISLT
                     KVLPAVMQLLNEKGEIVCLIKPQFEAGREKVGKKGVVRDKDVHIQVIEKIISFCRDEM
                     GLSILGLSFSPIKGPEGNIEYLLYLSKRKDLSDTAQRIDIGGIVLKAHELLR"
     gene            57335..58183
                     /locus_tag="PKI50_02820"
     CDS             57335..58183
                     /locus_tag="PKI50_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNT02681.1"
                     /translation="MKLGIIPNINKDKDLNVTRSILLWFANKDAVLMLESDIAEKLGY
                     KNSGFSREKIYSSSDVVIVLGGDGTLLNIARQSARYDVPLFGINLGHLGFLAEAEISD
                     MYLSLDKIMEGDYNIEKRMMLEALVEDDENNGKEFLALNDFSVTKGMTSRMITISTYI
                     NDSFFELYSADGVVISSPTGSTAYSLSAGGPIVSPELKVLIITPICPHTIHNRSIVVS
                     EKDEVKIVIGDENPEVMLSVDGQDSYRLNSGKVVRIRSSSFVTNLVKLKQRSFFDVLR
                     RKISER"
     gene            58210..58659
                     /locus_tag="PKI50_02825"
     CDS             58210..58659
                     /locus_tag="PKI50_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HNT02682.1"
                     /translation="MKISRHAKILEIIEKHPTETQEELAEELKKCGYNITQATVSRDI
                     KELKLVKVLDEDGIYRYASLKEQDSMLNERLVKVFAESVLSVDFAGNIVVVKTFSGAA
                     NAAAEAIDVLDFKEVVGTVAGDDTIFILIRNLENVEVVIDRLKKMMK"
     gene            58671..60353
                     /gene="recN"
                     /locus_tag="PKI50_02830"
     CDS             58671..60353
                     /gene="recN"
                     /locus_tag="PKI50_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNT02683.1"
                     /translation="MLLEMSIKNYVLIERQLIAFEPGLNVITGETGAGKSLVIDALAA
                     VTGGKFSKEDIRTGEEKASVEALFSLEGVWDIKNILEEYGIEKDADNSLVITREVNAH
                     GKSVCRLNGQIVTLSVLKRIAQYLVDIVGQNEYQLLFNSVKHGEYVDSFGSDEIYQIK
                     RDVKHLSDKHAELSEKLKSASGSASERERKLDMLRFQVEEIESARLKINEDEELRLRR
                     HLLVNAEKLLKGVNHVYNVIFSGDSNKRGALDMLSESAGTLRELAAADEKLETYKSRI
                     EEIMYQLEDIKGELRVYRDNIEFDDNEIDFLEERLDQINKLKRKYGSSIEEVLVYRDN
                     AAKELQELIDNEKNMAEIESEIEKVKDQYMIKARLLSGTRKAKAEILQKLIEKELQDL
                     NMYGSRFVIKILQDENIISPNGIDKIEFMISPNPGEPEKALSKIASGGEMSRVMLAIK
                     NAFSEVEKAPCVVFDEVDAGVGGQTANMVGQKIKAISKNAQILCITHLPQIASLTDNH
                     IFVDKKVQDNKTYTEIKILNFEERVEEIARMLGGNENIEASLEHAKQLVGKM"
     gene            60515..61819
                     /gene="spoIVB"
                     /locus_tag="PKI50_02835"
     CDS             60515..61819
                     /gene="spoIVB"
                     /locus_tag="PKI50_02835"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HNT02684.1"
                     /translation="MHKKNYRNVFLYTTLIITILCLTNVLFTIYSFPDEINVIQGQDR
                     KLPSSMVCNLEVIGDYKHSIDVNNNKIISSFLLMEDAGKGKAKANLMLLGLLPVKQVT
                     LNMIPDIKVVPSGEAIGVRIESKGVLVVGLSSITDTKGRRCSPAADAGFEVGDKIIQI
                     ADNQVEKERDIIDYLNNRHDKEERTKVLVERDGSRQDLYVKPVKCEEDGIYRIGLWVR
                     DNIAGVGTMTFYDPKSRMFGALGHGITDVDSGVLVDINSGKIIKSKIASIQKAKKTVP
                     GELVGVFYDSEDEYGIIYKNTSFGIYGKLSKKQKPERTKPVSIGLNSQIKEGPAKILT
                     TIEGNKVEEFDIEIQKAMRQRNSESKSMIIRITDEKLIEKTGGIVQGMSGSPIIQDNK
                     LVGCVTHVLVNDPTKGFGISIEWMLKEADIELVDSLKRASGE"
     gene            62159..62965
                     /gene="spo0A"
                     /locus_tag="PKI50_02840"
     CDS             62159..62965
                     /gene="spo0A"
                     /locus_tag="PKI50_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HNT02685.1"
                     /translation="MNLQKIKILIADDNKDFCIILSEYLTAQPDFEVVGVAKDGLEVI
                     ELIESELPDVVILDIIMPHLDGLGVLEKLNSMQIEKVPKVIILSAVGQDKITQKALTL
                     GAEYYVVKPFDMDIFSKRIRECFGIANNETERKNYIHPVAVASPAVTTPRELESAITN
                     IIHEIGVPAHIKGYVYLREAITMVVNNMELLSAITKELYPSISRKFNTTPSRVERAIR
                     HAIEVAWGRGRIDVINNLFGYTIHNVKGKPTNSEFIAMIADKLRIELKIS"
     gene            63121..64140
                     /gene="steA"
                     /locus_tag="PKI50_02845"
     CDS             63121..64140
                     /gene="steA"
                     /locus_tag="PKI50_02845"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HNT02686.1"
                     /translation="MKLQGKIKAGARTKNLVNFLCPGDIAVIRHDDIDEMAALAIANT
                     GVKAVINTGKSMTGRFSSAGAAVLLENNIAVIDVDLSLEMFKDDDMITVLGNDIIIRN
                     RIYKNVCTAVNWKYIGKRRQESKENEKYEILKFIDNTINLTALEVDKFISVNNYPELS
                     TRLKGRHAVIVVRNSGTLDELNALKAYIKKFNPVFIGVDGGADLIINSGYIPDLLIGD
                     MDSVSDIGIYKSREIILHAYEDGHCPCLKRIGGMNVPYKIFAVRGTSEDAALMLAYNK
                     GAELIVLVGGHNCMYDFISKGRAGMASTFIVRTLIGERLVDCKGFGIISASENEGKDA
                     QWAKM"
     gene            64125..64799
                     /locus_tag="PKI50_02850"
     CDS             64125..64799
                     /locus_tag="PKI50_02850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT02687.1"
                     /translation="MGEDVSVIIPAYNESDCILRTLEGLKGIENIKEIIVIDDGSTDS
                     TYELLKPLRDIILLKNEYNEGKGSAVRHALDYATNRFVALLDADICESAAEIYKLIEH
                     INPCGKSIIIGRLPAPARKGGFGIVKRLSSGCFRAMTSRNVDSILSGQRVLPLDFLKS
                     IELPSGFGLEFKLTLEAVRRGYEVIEVPVDMRHRETGRNISGFMHRGKQCIDIIDIIR
                     NEMKRL"
     gene            64816..65655
                     /locus_tag="PKI50_02855"
     CDS             64816..65655
                     /locus_tag="PKI50_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl pentapeptide
                     phosphotransferase"
                     /protein_id="HNT02688.1"
                     /translation="MDLIVIAAVTLIIAHYLIRNIIKMLCRAESRICISSNYKGKRIP
                     SIGGIVFVPIQLAAVFQLLLQRPEHSYGYLSYMFLMLSMGFAGVIDDLAGDKRIKGLI
                     NHVRSTLKSNMTTGFIKALIGFLAACTISFRTTGSFAELAADVLVITLSANAINLFDM
                     RPGRAVKVFLAISLMILSSSAGRPAEALPLIILVMTVLIYMSYDLREICMLGDTGANI
                     LGISLGFYSTLFLSFSSKLMLLLLLILLNIAAERLSITALISKSRILSYLDSLGRRRT
                     DNR"
     gene            65652..66722
                     /locus_tag="PKI50_02860"
     CDS             65652..66722
                     /locus_tag="PKI50_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HNT02689.1"
                     /translation="MIDIFKAQVKEIIYRDVYISKFRIERNSMLCNCINYEMLTGSVE
                     VNDTVLLNTTAVQLGLGSGGYHYVLANLSKDSFSNIGLGHIMKLRYTPMQINCMAAEA
                     QESPYHKIFNDFESLRGLPVITGTLHSMLAPIALTLKHIAGKERIVYIMTDGGALPIW
                     MSETVKRLRRSGVLYGAITFGNAFGGDLECINVYTALIAAKEIMKADAAVITMGPGIA
                     GTGTRYGFSGIEQGYIIDAVNNLDGKAIAVPRISFADSRSRHYGLSHHTMMTLGKLCC
                     TKALIALPILEKSKDDLIRKQLEESGVLSRHRLIRLDGTEVEKLLETEKRQLERMGKS
                     FCQDKEYFITCGVSALLAMDSN"
     gene            complement(66715..66852)
                     /locus_tag="PKI50_02865"
     CDS             complement(66715..66852)
                     /locus_tag="PKI50_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02690.1"
                     /translation="MVKYVYKNGFHFVGKFNELMEQLNMIENKQITLREYILSYRYRL
                     N"
     gene            67008..>67355
                     /locus_tag="PKI50_02870"
     CDS             67008..>67355
                     /locus_tag="PKI50_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNT02691.1"
                     /translation="MPDSLNDSIRRHLADNIWIYLIVMFLFILGISLGALAVNNIDEA
                     AKTDAITYLEGFLDLTNQNQLQSEAILKQSIKYNLYYALILFFSGVVYIGILVIPILV
                     AFRGFCIGFSIAFL"
BASE COUNT        21079 a        12071 c        17200 g        17005 t
ORIGIN      
        1 gacgtttctc ctgtcccact tccagtggga cacgcgaaac gtccccgtgt ccccgtgtcc
       61 ccgccgcatt cttcttcaat tctgccacaa tatttatata ggatgaacag tatgaacaat
      121 atttggggat caaagaaggt caatataaga cttattcata ttatcataat tgcagcattg
      181 gtttttattg tgctcaaata tggcattgcc atcagcacag tgataaagcc ctttataatt
      241 gcggcagcct tctcttacct gctgaaccct atagtgaagg tttttgaaaa aaaaggcatc
      301 agaaggattt tcggagttct tattgtatat ctgattttta tagctataat tatgctcctg
      361 tcttttgtgc ttgttcctaa gctgataaag gaaatcagca cactggttgt caacatcccc
      421 caatactcaa gacagatgca ggagcttttt cggcaattcc aggacggata catgagaagc
      481 gggcttccgg agagttttaa ggatgtactg gatgataaca tacttatgct gcaaagcatg
      541 attctcacac ttttgcagaa cattgccaac ggcataatag aaatcttttc acagtttttt
      601 aatatagtaa tagttccggt gattactttt tatatgctga aagatgcgga gtacttcaag
      661 aaccaattca ttcttgtact gccgaaggca aagcgcgcga aacttattat attaataagg
      721 gatattgata atgtttttgg taaatatata agaggacaga ttattatagc cagctttgtg
      781 ggtgtactga caactgtcgc ccttatactg attaaagtaa aatatgcctt catacttggt
      841 atctttgccg gtatagcaaa cataatacca tacttcggac cttttatcgg gattatacct
      901 acaatattgt ttgcattact ggattcaacc ggtaaagcat tgtatgcggc aggagtcttt
      961 attctgatac agcagctgga aagcggattc cttacaccaa ggataatagg aaagagtgtg
     1021 ggaatacacc ctgtttatgt tataatgtcc cttatagcgg gaggtcggct ttttggaatc
     1081 atagggctta ttatggctgt gcccgtattg gctgcaatta agctgacact cagacatgta
     1141 ttcagagata ggcaggggag ttaaggactg cttaatggtt gaatttgagt tttaatgtga
     1201 attattgaca aagcttcccg gctaatatat aatcttcgta tatatttaat attgtgttga
     1261 tgcagaggag taaacagact gattttcaat atgctgcaaa atatggcagc ataagcagag
     1321 aagggatggc tggtgagaat cccagggaat aagctgttga agctgctgct gagcatcgta
     1381 aagacgctgc aggcgttata ctgcatggct aaagccaaca aagcggccct ttcgggcaac
     1441 tagggtggta ccgcgaagtt ctcttcgtcc ctaaccggga tggggagttt tttttacaaa
     1501 aatatacggg aggaatacac atggaaaagt taggacttaa tgaagtaaga gaaaaattcc
     1561 ttagcttttt tgaaagcaag gatcatttaa gaatgaaaag tgcatctctt gtgccacaga
     1621 gtgacaagag cctgctcatc ataaactcgg gtatggcccc cttgaagccg ttttttaccg
     1681 ggcaggccgt acctccgagc ttaagggtga caacctgtca gaagtgcatc agaacccctg
     1741 atattgaaag ggttggaaag actgccagac acggaacctt ttttgaaatg ctgggtaatt
     1801 tctccttcgg agattacttc aaggatgagg caatagcctg ggcatgggag ctgtgcactg
     1861 aggtatataa gatgccggaa gacaggcttt gggttactat ttatctggac gatgatgaag
     1921 cctttgatat ctggcataac aagataggat tgccgccgga caggattgtt agaatgggta
     1981 aggaggacaa cttctgggaa atcggccttg gcccctgcgg accttgttca gagatatatt
     2041 ttgacagagg gccgggtaca ggctgcggta aggaagactg caagctgggc tgcgactgcg
     2101 acaggtatat ggaattctgg aaccttgtat tcacacagtt tgataaggac gaagaaggca
     2161 attacaacag actggcaaag ccaaatatag acacaggcat gggattggag cgaatggctg
     2221 cggttatgca gggagttgac aacatatttg aggtagacac tataaagtat atattggaca
     2281 gcatctgcag ccttgcaggg gtggaatacg gtgcggatcc gaaaaaggat gtttcaataa
     2341 gggttattac cgatcatatc agaggagtta ccttcatgat atccgacggc atacttccaa
     2401 gcaatgaagg cagaggatat gttcttagaa ggcttctaag aagagctgca aggcatggaa
     2461 ggctactggg tgtgaccgat tctttcctca gcaaaattgc cctgaaggtt atcgaagtaa
     2521 gcggagacgc atacagcgaa ttgagggaaa agaaggatta tatcctaaat ataattgatg
     2581 ttgaagaaaa taggttttat gagacattgg accagggact ttcaatactt cagggttata
     2641 ttgacgagtt gaagctgaca aatacagaca cattgtcagg agaaaatgcc ttcaggcttt
     2701 atgataccta cggcttcccc ctggatctga ctaaagacat actggaggag cagggactat
     2761 ctgttgatga ggaaaccttc cagaaggaga tgcagaacca aagggaaagg gcaagggcag
     2821 caagggctga cagcagcgct gaaagctgga aggaagatat atatgcaaag atcgactgga
     2881 gcataaagac taagtttgtg ggatatgaaa gcttttccgc aaaagcgatt gtcaaagcaa
     2941 tagtaaggga taatgaactg gaagactcag ccaatgaagg ggatgaaata agcgtagtac
     3001 ttgatattac acctttctat gcggaaagcg gcggacaggt agccgatacc ggaagaataa
     3061 tcggggaagg cttcgagttg gatgtaaaag actgtaagaa ggtcaccggg gaaaggtttg
     3121 cccatgtgtg tacggtaatc agcggtgaaa ttaaggtcgg agactcctgt atagcagaaa
     3181 ttgattcaaa aaggagaatg gctaccgcca ggaaccactc ggccactcat ctgcttcata
     3241 aggcattgag aaaatacctt ggcactcatg ttgagcaggc aggctcttat gttacgccgg
     3301 agcgtttaag gtttgacttc actcacttca aggcgatgac aaaggaagag cttgacagga
     3361 tagaaagaga agtaaatgaa gcaattctgg caagctatga agtaattaca aaggaaacct
     3421 ccataaatga ggcgaaaaag cttggagcta tggcgctttt cggagagaaa tacggtgaag
     3481 tggtaagagt tgtcaaaatg ggcgactatt ccattgaact ctgcggggga acacacttga
     3541 aagccacaag ccaggcgggc ttcttcaaaa taataagcga agggggcata gctgccggag
     3601 tcagaaggat tgaagcggca acaggagaaa aagcttatga atttattaag gaagaagaaa
     3661 aactgatgaa tgagctgtgc gggaaactca aggcccatcc aagcgaatgt gtcaagaaaa
     3721 tggatacagt actcaagcag cttaaggata cggaaaagga agtatctgtg ctgaaatcaa
     3781 aaatggcggc ggaaatgctg gatgagcttg cagccggggc acaagaggag aaggacataa
     3841 aatatcttgt aacaaagctt gacaacatgg gtattgacag cctgagagaa atgggagaca
     3901 aactaaggga taagctggga acctcctttg tgatgcttgc ctcctttgac ggggaaaaga
     3961 cagtcctggt tgcaatggca tcaaaggatg cggtttccaa gggagtacac gcagggaata
     4021 tcattaagga ggctgcaaag gctgcaggag gaagcggcgg cggaaggccg gacatggcac
     4081 aggggggaat aaaggatctg gataaagtcg cagcagcttt ggacgcagtg ccggagatac
     4141 tgaaaaacat ggtgaagtag taaaataaca agaggcaggg ggacatgggg acgtttcggg
     4201 acacggggac atttcgggac acggggacgt ttctcctgtc ccactgaaag tgggacagga
     4261 gaaacgtccc cgtgtcccga aatgtcccca tgtccccaca tatcatctgc aaactaaaag
     4321 ggtggaaagc ttgcttccca ccctttattt attgacttct atctttgtgc ttcgtactgt
     4381 ctcagaagct tggcaaacaa ctccggaacc tttgccattt tatccggtgt ttctacgaag
     4441 gaaatacgga attgtgtgct ccccgggttg tattctcctt ttttagtatt atagaatcct
     4501 ttcattggag ctaccagaag agttgtctca accccatcca ggttgaccga tccttctcct
     4561 gcgcagtaca gtacgaactc agttgcatca aatcccggtt ttacaacatt tctgacatcg
     4621 attaccgagt agatggacgc atcagggctt gaaactatta agcccggttc ctgtttcttc
     4681 agtccgttat acacattaaa tatcaattcc ttgtaatatt ctcttatact cttgcaccat
     4741 gcagctattt gctccttgct ttcatgagcc agtgcgccga atatatactg gccgattgca
     4801 ttagcgcaaa gatttgcagt atattcagct actgaacggt tattgaattc ggggctgtct
     4861 gtaatcaggg caccgattct cagtccgcag gcattccaaa cctttgatgc agtttcaatg
     4921 ctcattcttc ttccttcgat accgggtaca tccgcatcgg taactcccca gatgcttaca
     4981 agcttgctgc cttcctcata gaagagttcg cggtacgctt catcgcttac aaaccacata
     5041 ttgtacttta cgcacaatgc cgcaagccct ttaagggttt cataatcata gaattggccg
     5101 gtcggattgt cgtaaggtat aacaagaagt gccccgggat tatacttttt aatagtttct
     5161 tcgatttcgt tcaattttgg aaggctgaac ttgccgtttt catttaatga acgggttacc
     5221 gtaactattt ttcgtccgac tctctcggca aaagaaatat agttggtata ggccgggtca
     5281 attaccataa gaggtttttc atcagtacct gccggtccgc aaacccccaa taacagtaat
     5341 tccattgcag cagatccgcc gtcagttatc tgaacataca gcttgcttgt gtcgaagcct
     5401 tcgcatttga ggatattctt aaatgcgtcc tgacactcta taaggccggc agttgttgta
     5461 taccttatga caccttttgc aaaggggctt tcaggtgcat caagcgaaaa cattcttttt
     5521 tgcattgcag gattggtagg aagtgaaaca ttacctatac ctacattaat tacggcctgg
     5581 ggtttgacct tccgctcctc atatttcatc tgggccagtc ggacacttga cggtgtcctt
     5641 gattcaaagt gagcggataa tactggttta ctcatttctc taaaacaccc tttctcattg
     5701 atatttgttt attattacta taatattaga aattacagtg ttttttttta gctctatact
     5761 attttaacac taattgataa tattttcact atttttttaa attttaatta aaaccggtta
     5821 aagccctcat tgaaatgttg ttataaaatc cggcttatag gggaaaatat tgtatagcgc
     5881 aatttataaa taagcaaaag tatggttatg aggtgacagg atgaaggctg taataatggc
     5941 cgggggagaa ggtacaagac tcaggccggt gacctgcgga ataccaaagc caatggtgcc
     6001 ggttcttaat aagccggtaa tggaatatac catagagctg cttaaaaagc ataatataac
     6061 tgatattgcc gcaacactgg catactttcc cgcagtgatt accgattatt tcggagacgg
     6121 cggagaattc ggcgtaagtt taaggtacta tattgagcat acccccctgg gaaccggcgg
     6181 aagtgtaaaa aatgcagagg atttctacga tgatactttt attatcgtca gcggcgatgc
     6241 cctgactgac atagattttg aaaaagcact ggaatttcat aaatacagaa aatcgaaagc
     6301 tacgctgata ttgaaaaaag tacccgtgcc cctggaatac ggagttgtta ttattgacga
     6361 agaaggcaga ataacaagct ttatggaaaa gcccagttgg ggagaggtgt tcagcgacac
     6421 cattaatacc ggtatatata tacttgagcc tgaagtgctt gactattaca gtaagggtga
     6481 taatttcgac ttcagcaagg atttgtttcc aaagctgtta agagataata tccccatgta
     6541 tggatatgta accgatgact attggaatga catcggtgac ctgagagtat acaaggaagt
     6601 aaatttcgac attttgtcag gaaaggtcaa gacaacaatc ggatacaggc agcatgggga
     6661 gggaatatgg ataggtgacg gctgtgagat ttccgacagc tgtaatctta ttgcaccggt
     6721 gcttatagga gacaactgca gaatcaaaga caggacagta atagaagcct caatattagg
     6781 caatgacacg gaagtacagg aagcaacctc cataaagaaa agcataatat ggaagaattc
     6841 aagtatagga aagaatgtcc agtgcagggg aggagtaatt tgcagcggtg cagcagtaaa
     6901 aagcggagtg catatttttg aaaataccgt tataggcagt aatactactt tggaaacagg
     6961 cgcgataata aagccggata tcaagatatg gcctgaaaag gttgttgagg aagatgccgt
     7021 agtggcccaa aatcttgtat ggggtacgaa agcatcaaaa actttattcg gccacaggga
     7081 tgtcagcggg gatgtaaata taaaccttac gccggaattt gcttcgcggc ttggttccgc
     7141 tttcggctca atactgaagg atgattccgg aatcattgtc agcagcgacg gttcgaactc
     7201 tgctgagata atgaaggaat cactgatatc cggagtgctg tctacaggtg taggagtaat
     7261 aagcattgaa aacgcagcag tgcctataat gaggttcgcc gtcagattct acaaggcaga
     7321 aggcggagtt catatatgga aggattatag tgataagaac aaagtgcatc ttgaattctc
     7381 aggcagcaaa ggaataagca tagacagatc cgttgagaga aaaatcgaga atagctttat
     7441 cagggaggat ttcaaaaggt gcaatgcaga tagaatcaag gacataataa gtgcgggtaa
     7501 tttcacggat ttctatatcc aaagcggcat aaacatgctg aaatatacag acagaataag
     7561 gagttccggg attaagctgg ttgcagcttc aatatcctca cagatagccg gtttagctca
     7621 aatgtatcta aaaaatatgg gttgttcagt agagagggat agcgtttggg acaggtataa
     7681 gatgccggcc aaggataata tcagtgccgg agcatactac gaacatatag ccgagctgac
     7741 tgtcaagagc gggtcatgta tgggagtagt attcagtgag gatggagaaa gttttgtact
     7801 tattgaccat aaggggagga tactggataa ggataagcag attgctttga cttcattaat
     7861 aatacttaag acaggaatgg acaaaaggct tataattccc catacggcat caagagtaat
     7921 agaggaaatg gccaagtgct acagcataga ggtagtgaga accaagaata cccctgcggc
     7981 aattatgaga gagatgtttc agcaaaatac cgggatggag gaatctttgt tccaatatat
     8041 acttaacttt gacggaattt ggggtatagg aaatctggtt gactttattg caggcaataa
     8101 tgtaacactg tatgacctgg taaatgagct tccccatttc catatggaga aaaatgaagt
     8161 aaagtgcgac tggcagcata agggaagggt tataagagaa atcattgagg aaaacaagaa
     8221 cagagagatt gaactctttg aaggggtaag gataaataat gaaaacggat gggttctcat
     8281 acttcctgac agcgaaaggc ccgtgtgcaa tgtttatgcg gaaggagcat ccgaggagta
     8341 tgcaagagag cttgcagccg agttcaccga caaaataaaa aaaatagtct ctgctgaaga
     8401 taagtagcag aatgcggcgt aaatatgaac tgaaggaatg gtgaggatta tattgaattt
     8461 ggtagctaat tatttttata tggcagcatt tgttcttgct gctgttttga tatacttgct
     8521 cagcaggctc ctatacttta ttgacagaga tgaaccggaa gtatatgatg cgatacttaa
     8581 tgcggaggaa ttggaaaaac acgcattgga attggcacgc aagcatgttg tgggtaagag
     8641 ggcaaaacat tacttcagtc ttgagggcag gatgaataaa aacttccggt tcattacctc
     8701 cgtctataag gatctgaatg aatatgcaaa tgaaagaagc agtgttactc cggctgcaga
     8761 atggctgctg gataattttt atattattga ggaacaggta aaggaaataa ggcagaactt
     8821 atccaggaag tattattacc agctgcccag gctgaagtcg ggattcctga agggatatcc
     8881 gagggtttat gccatagcct tggaattggt ttcccatact gacggcagat ttgacgataa
     8941 gcagctggta ggcttcgtat ccgcttatca gtcccaatca ctgctttcaa acggggaact
     9001 ctgggctata ccgataatga tcagaatggc tcttattgaa catgtaaggc atgtctgtga
     9061 aagcatttca ttatcgcagc agcagtggca taaagctgaa aagcttgctg aacagattct
     9121 ttcatataag gacagaaagc cggaagagct gcttgccatt gccaaggaaa gcatgaggca
     9181 gcttgagaat atcagcccct cctatggcga acacttgctt aatatgctta aaaagcacgg
     9241 catagaaata gcccccataa tacactacat tgatgaaagg ctgtccgagc agcataccac
     9301 ctcggaaagc atcacccagc tggaacacaa ggaacaggct gcccgccagg tttcaatggg
     9361 caattccatt accagcctga ggttcatatc cactttggat tggaaggtga tttttgaaga
     9421 actcagccag gttgaacaaa ttttgaggca ggatccggca ggtatatacc agaagatgga
     9481 ctttgcttca agggataaat atcgccatga gttggagata ctttccaaaa agcttaaggt
     9541 atcggagata aaggttgcaa gaaaattaat agagtgctct gtggaagata acaggcatat
     9601 cggatattac ctgttcaggg agcagaagga gcagctgctg gacagaattg cccgcaaggg
     9661 gatgtttatc tattccccgg caggggttta tttcttttca attattactc taacggcgtt
     9721 cttagtgttt ttgatgctta actactcata cagggaaggt gcagcggggt tttggacaca
     9781 gcttgtgata gcccttgcag tattaattcc ggcaagtgat gtttcggttt cggcaatcaa
     9841 ttggatactc gtacatataa agcccccggc atttctgccg aagcttgagc ttaaggacgg
     9901 aataccggaa gatgcagctg caattgtagt gatgcctaca ctgataacaa agggcagcag
     9961 tgctgtaaag ctgcttctga agcttgagga atactatctt gcaaacaggg agggaaacct
    10021 ttattttgcc ttgcttggag attataagga cagtgacggg gaaaaatccg aaggggatca
    10081 ggagattatt ctggcagcaa gcgagaaggt aagggagtta aatagaaaat acggaagcga
    10141 tgaagataag ttcttttttt tccatagaag aagggttttc agcaaaaccc aaaacagatg
    10201 gatgggctgg gaacgcaaga gaggcgccct ttatgaattt aatgaactgc tgctgggagg
    10261 caaaaaagca tccttcgagc atatcgaggg aaatatcagc gcacttaaag atgtaaagta
    10321 tgttattact attgattcag acacaaaact cagtattgaa acagcaaaaa agcttatcgg
    10381 cacaattact catcctctaa acaaagcagt tgtggatgag ggcagaggtg tcgtaaaaga
    10441 aggctatgga ttgctgcagc cacgcatcag tgtcgacata gtaagtgcaa atacctccag
    10501 gtttgcaaga gtctttgcgg gacagggggg catagacatt tatacaaatg ccgtctctga
    10561 catatatcag gattactttg gagaaggaat atttacaggt aaagggatat atgagcttgc
    10621 agtttttcag gggattttaa gaaatatgat acctgagaac acagtattaa gccatgacct
    10681 tattgaagga gcccatgtca gaacaggact tgttacggat attgagctta ttgacggata
    10741 cccttcaaaa tatagtgcca gctcaatgcg ccttcacaga tgggtaaggg gtgattggca
    10801 gctgctgcag tggctttttt caaaggtgcg cagtgcaaag ggagagattg tcaggaatcc
    10861 gctttcaccg ataaacaggt ggaaaatact ggataacatg agaagaagcc tggttccgcc
    10921 caatctggtt ttgctgtttt ttctttccct gtggttaatg cccggaaatg ccctggtatg
    10981 gctgctgctt actttaatag ccgcagtatt cccgcttttt gtgagctttg ctgaagtggt
    11041 aatgaccaaa tatgacagga ggatgaatga ggggtcggcc cgcggagaat tgggaactct
    11101 gaaaaggccg ttcctgcagt cgctgcttgc actggcattt cttccccatc aagcccagct
    11161 tatgctggac gcaatagtaa gaaccttggt aagagctctg ataacaagaa aaaacctgct
    11221 tgaatgggtc actgcggcag atatggaaca cgcgctgaaa aatgatatgg caagctattg
    11281 gaagcgtatg tggttatgcc ctgcgtccgg ggcagtttta ttgctgctga gtatgttcag
    11341 aagcccggaa gcaatcatcg cagtgcttcc tgttgctttg ctgtggattg ccgctccggc
    11401 aacagcttat tgggttagtg agcctctggc ggagaaaaaa aatgaactga gcaaaaaaga
    11461 gattatggaa ttaaggatat tggcccggaa gacctggagt ttttttgagg atttcgtaag
    11521 tgcattggat aattaccttc ctcccgacaa ctaccaggaa gaccctccga acggagtagc
    11581 gcacaggaca tccccgacga atataggttt acttcttgcc tcaataatat ctgcaaggga
    11641 tctgggttac ataagcacat gggaaatgct cgaaaggctt gacaatacaa ttactacaat
    11701 agagaaactc aagaaatgga gagggcacct gtataactgg tatgataccg ttaccttgga
    11761 ggtactgagg ccaaactata tttcaacagt ggacagcggc aattttatcg gatacctgat
    11821 gaccttgagg caagggctgc tggagtataa gaacaaaccc tcaattgatt cgggaatggt
    11881 tctggggctt aaggacacct cggagcttgt aaagctggag gatgaacaat tgagcctgcc
    11941 tgatgaaatt tttgacaagg ctttggaagg ggatatgctt gagcagcagg gttttattga
    12001 gctgctgaca aaaattatcg ggaaaagtga tttgaagaga ttcaactggg gcatcaagct
    12061 ccataagtcc gcaatcactt ttctcaagga aataaacagc ggaattgcgc aggaaaacaa
    12121 tgaggagctg aaaatcaaat cggaagggct gattgaaagg attgacaatc ttattgaaga
    12181 aacatccttc cttcctctct ttgataataa aagaaaattg ttttcaattg gctataatga
    12241 ggaggaggaa cagcttacaa agtcctatta cgatcttctt gcatcggaag caagacaggc
    12301 aagcctgatt tccatagcca ggggtgaggt caagaaggaa cattggttca tgctcaacag
    12361 gacattgact gaggttgacg gcttcaaggg cttgctttca tggacaggta caatgtttga
    12421 atatcttatg ccccttctta ttatgaagga ctattcggac acactgctcc atgagacata
    12481 tgacttcgta gtaagatgcc aaaaggatta cggaaataag aggaaagtac cctggggggt
    12541 ttcggagtcc ggctactaca gctttgactt caggctgagc tatcagtata aggcttttgg
    12601 aataccgagc ctggggctta agaggggact gattaatgat acggtaatag cgccctatgc
    12661 tacggttctg gccttgatgg tgaatccgaa agagtccttc aaaaatatct taactttaaa
    12721 ggaggaaggt ctggatagtc cttatggctt ttatgaagcc atagattata cccccgaaag
    12781 attaaaaagg ggaaaaagaa gcagtatagt cagaagctat atggctcatc atcagggtat
    12841 gagctttatg gcgctgaata atgttatcaa caattatata ctgcaggaaa gattccacag
    12901 ggaacccgtc ataaaagcgg cggaaatact catgcaggag aagataccct caaaggtgat
    12961 tttcgcaaag gactacaagg aaaaaatcga accctttgaa gtagttgtga aagaagacgt
    13021 agaatatacc aaagttctcg gactgaacaa caatatactt ccggaagtgc atatgctgtc
    13081 aaatggggat tattccgtaa tggtaacgga caacggttcg gggttcagca agtacaggga
    13141 tttggcagtg accagatgga gagaggacag tgttttcaac tattccggca tgttcttcta
    13201 tttacaggat atcgattccg gttcggtttg gtcaaattcc ctggctcctt gcagtgtact
    13261 tcccaacaag tacaaggtag tgttttctca ggataagata tccatttcca gaaccgatga
    13321 cgatattgac actaacacgg agataattgt gtcgccggag gatgatgcgg aagtaagaag
    13381 gcttaccctt acaaatcaca gtcagcaggt aagaactcta gaagtaacaa gctatttgga
    13441 agtttcaatt gctcatcagt ctgccgactt ggcacatccg gccttcaaca atctgtttat
    13501 caataccgag tatttgcaca aatacaacac actgctgtca gtaagaaggc ccagggagga
    13561 aaagggaacc cgcatgtggg catttcatga gctgacagtt caggaggaag ttctcggagg
    13621 aatacaatac gaaaccgaca gggcaaaatt cataggaaga ggcaatatgc cggataaacc
    13681 tgcggcagtc ggagggagcc atcctctttc caataccgtg ggaccggtgc tggatcccat
    13741 aatgagtctg agatgcaggt taaggctgca gcctggccag actgtcaaac tgaattacat
    13801 aacgggtgta tccgacagca aagaaaaaat aatgctgatg gcagagaaat atcatgactg
    13861 ttcgtccatt gacagggctt ttgagctggc atggaccaga agccaggtgg aaatgcggta
    13921 cctgggactc aagaaagagg agatagaagt cttcaggaat atgatgtccc agatattgta
    13981 tctaagcccc ctcagaagga agcaggaaag ctccataaag ggaaacagga aaggacagcc
    14041 ggggctttgg gcatatggta tttcaggaga ttttcccata gtagcggtat ttatcagcag
    14101 ggcagaagag ctggatcttg taaaagagct tataaaggcc catgaatact ggagattgaa
    14161 gggcttgttt gttgacgtcg ttattatcaa tgaagaggag agcagctact taaggcctct
    14221 gtacaacatg ctacaggata ttatttcggt aagccatgca agagatatga gggataaggc
    14281 aggaggagtg acaataaagc aggggtactc catgccggaa gaggataagc agcttattgc
    14341 ggcagcagca aggatagtat tgaagggtaa atacgggccc ctttcggtac agacaaaagt
    14401 gagaaacggt aaaaatgcat ttgagtacaa gatatggaag agcaaaggga aaaagcataa
    14461 caagtacgat ataaaaaagc ttgaaattaa gaacttcaac ggtttcggag ggtttgacaa
    14521 agagggcagg gaatatgtaa tcaagcttac tgaaggcatg aatactcccg taccctggtg
    14581 caatgtaata tccaatcaaa aattcggttt tctgattact gaatccggtg cctccacaac
    14641 ctgggcggaa aacagcagag agaacaggct gaccacctgg tccaatgatc ctgtcattga
    14701 tccctcaggg gagataatat atatgaggga tgaagaaaca ggtgaaatat ggtcggttac
    14761 ccctcatccc ataaggacga aaactcctta tattgtaagg cacggcttcg gctattcggt
    14821 tttcgagcac aactggaacg ggatcgagca tgaactgact gaatttgttc cggtctccga
    14881 cactgtaaag ctgtgcatat tgaagctcag gaacggctct aatacaaaaa tgaagctgtc
    14941 ggctatatat tatgcaagaa ctgtattggg tgtatatgaa caaggtacgg ttcagcacgt
    15001 ttacaccgaa ttgcacagca acggcgccat tcttgccaga aacaactata ataccgattt
    15061 cccgggaagg atagtatttc tggatactaa tgcaaaggaa aggtattaca cgggagacag
    15121 aagggagttt ttgggcaata taggagggct taagcatccg gaagcgctgc ggaaggataa
    15181 gctttcaaat acacttgggg caggctttga cccctgtatg tgcattcaaa cggttataga
    15241 gctgaagccc cgggaacaga cagaagtggt attcatgctg ggccaggggg aaagccttga
    15301 agctgtgagc gcatacagtg aaaggtacag aaatgtgaag agggtatata aggctttgga
    15361 tgaggcaaaa gctttctggg aagatgtttt aagaaaagtg acggttttga ctcccgatga
    15421 aaatatgaat atactgctga acggatggct gctttatcag acattaagct gcagaatatg
    15481 ggcaagaacg gcattctatc agtcgggagg agcttttggc ttcagagacc agcttcagga
    15541 tgcaatggca gtaattgatg tactgcctga tattgcaaga aagcagattc tgctgcacgc
    15601 ttcccatcag tttattgaag gggatgtgca gcattggtgg catcccggct ccaataaagg
    15661 tatacggaca agattctcgg atgacctttt atggcttccc tatgtaacag taaattatat
    15721 taacaggaca ggagatgtgc aaatacttga ggaaagggtt gaattccttg aggatgaacc
    15781 gctgagggaa ggagaggatg aaagatacaa gataccttgt gtatcagatg tcagtgcttc
    15841 actgtatgac cactgcgtaa gggcaattga aagatcgttg aaattcgggc atcacggtat
    15901 acccttaatg ggatcgggag attggaatga cggtatgagc acggtaggca acaagggcaa
    15961 gggtgaaagc gtatggctgg gttggttcct gtatgacata cttatgaatt tctcggagct
    16021 gtgcaggatg agaagggatt tgaacaaagc agaccgatat ataaacatag cgaaagaaat
    16081 tgcaaaggcg caggaggaaa atgcatggga cggaaattgg tacagaaggg catattttga
    16141 taatggaacg gcccttggct cggcggaaaa tacagaatgc aaaatcgatt ccatcgcaca
    16201 gtcctggtca gtgatatcgg gagctgcaaa tcttgacagg gcaagggaag ccatgcaggc
    16261 agtagaacag taccttgtaa agtatgatga agggattgtc aagctgctta cacctccctt
    16321 tgatgaagga gatctggagc cggggtatat aaaaggctat ccccccggtg taagggaaaa
    16381 cggaggccag tatacccatg cggcaacttg ggtgattatg gcttattcaa taatgaaaaa
    16441 tcctgatata gcctggaagc tgtttaatat gctgaatccc atcaatcatg caaatactat
    16501 gatggaagcc tcaaggtaca aagttgagcc atacgtaatg gccgcagatg tgtacgccgt
    16561 tcctccccac acgggaagag gggggtggac ctggtacaca ggtgcatcgg gctggatgta
    16621 caatgtagga atgcagcata tactgggatt caggaaaaaa gcaggcagga ttgtgatgga
    16681 accatgtata ccaagggatt ggcctgagta caatattagg tacagacaca acaagaatac
    16741 gatatataat ataatagtaa gaaatcagaa caggagcggc aatgcaaagg cagaaatcat
    16801 cctggacgga aggaagcttg aaggaaatga gtttccgctt gccgaagacg gagcggaaca
    16861 tacagcagag gtcatcctta catgacatta cggttacaca tgcaaacatt tccttgaaat
    16921 gaattattat ctatattatt atatatatat atgtgaacct ggggggtgta ataatgagcg
    16981 aaagattcga agacacaatg aaattcaagg ttgagacgga taagaagtat gaagcatcgg
    17041 atatacttct gtctgtatac agcgcactta aggagaaagg ctacaacccc atcaatcaga
    17101 tagtgggata tatactttcg ggagacccta cctatattac aagccacaac aatgcaagaa
    17161 gcctgattaa aaagcttgaa agagacgaac tccttgaaga gcttgtaagg gcttatgtcg
    17221 atgggaagta gctgaaatac ggttcaggag agattagttt gaacaaaagc ttaatcgctt
    17281 ttttttccat tgctgcgtta agtgcagcaa ttattgcttt gcaggatgta aaggcttatg
    17341 aaatttttgc atccggagaa gaggaatacg gcaaggtagt gatgatagtc ggagattacc
    17401 tggatatcaa ggatatcggc aggatggaga gcctttacag actggccggc aacagccgtg
    17461 ttgccctcat aaacaacagg cagccgggga aagccgatgc agccaaatca aaactcataa
    17521 tcggatccgg gaaaaaactt gagttggatg ccgatatggt ttcggaaggc agaagggaat
    17581 ctttgacggg tagtctcgta tatacgggta ttaacaggat caaagacaga aatgccgaca
    17641 gtgaatataa gaaatacatc ggatacatag gcgatgcaat taataagaag ggcgggactg
    17701 cctgctatat cggaaatgca gatgcagaga agccgaacaa aagttctatg ctaattgctg
    17761 cggacagcag cggtgaaatt gattcaggag agacagagga tgctattata gaagatgctg
    17821 ctttcccggg gggaaaaagg actgattatg acaggctggc ggaattgtac aagcaatatc
    17881 tgccggcttc ttcatttatt gtaatagaga caggggatat ggaaaggctg gaagccttta
    17941 aaggctccat gtccgaggaa gcctggcaat catgcaaaca ggatgtgctg gaaaggattg
    18001 acggatttgt aaagaagatt gtcagttacg ggggttttaa aacacttgta ttcatatcaa
    18061 cctacccttc gaaatcaaat gcggaagcgg gcaataaact gactcccatt gtggtttatg
    18121 aaggaaggga aggagtgctt tactcaaaca atacaaggcg gagcggcata attttgaata
    18181 cggacattgc ggattatgtg ctctgtaagc tgggatacct gactgaatgt gccgtcgagg
    18241 aattggataa aaaagcttcg ttaaatgctt taaggtatat ggacatgagt attttaagga
    18301 cttccgttct gagaactcct gttcttactt attatgcggt gtctgtgatt gcagtactcg
    18361 ggattctgtt tggcgcggct gtttttctcg gaaagaaagc ggggcatttt tggtctggtt
    18421 ttgggattat tactgcatat acaatgcttt cgattccaat cgcttttctt ttaatacccg
    18481 catcttatac ggattgggga tccgccgaat attcggtgct gactgccgcc gcttctgcag
    18541 tattgtcaat attgcttcac ctgaccttaa ggaacaggct gaaggttata ctggcaatct
    18601 gcctgctgct gcaggcagga cttgccgctg atatacttac aggcggcgaa ctgataaaaa
    18661 agtctgtgct ggggtatgat cccataatcg gcgcaaggtt ttacggcatc ggaaatgaat
    18721 acgcagggat gttcatcggt atttccctta tggtctttgg ctgtgtttcg gagctgagag
    18781 gacggagatg cggcaaggct gccgcagctg cttatttttc tttttgcacg ctccttcttg
    18841 gattgacgtc tcttggcgct aattttggag gggcagttgc aggggttttt ggttatttac
    18901 ttgcttattt cctggttttt gatataaaat ttaacagaag aaatattttt acgggagcac
    18961 tgattttggg atcggctgca gcggcattgt ttattgcgga cagcctggga ttggccaggc
    19021 aatcccacat gggagccctt ataaaggaca cccgtgccaa tggcgttgga gttattacct
    19081 ctgtgataag caggaaaata tcaatgaatc tcaggctgat gagatatacc atatggacaa
    19141 aagtgctgct gtgcataata ggggcaatat ccataatgtt ttacaagcct gtaaaacaga
    19201 tgttctatat attcaacagg tataaatatc tgaaatactc atggatgagt attgccgctt
    19261 cagccgccgc aggcttcgca gtaaatgatt caggcattgt agtagccgca acggcgatga
    19321 tatttatggc atttaccatg ttgattatgt gcataggaga agggaaggcg gtctgaagat
    19381 ggattacagg attttgggaa gcacagggct gagagtatcg aggctctgtt tcggaagcct
    19441 caccatcggg cctcttcaag cggggcttga tattgaagaa ggggcatcgg tgataagggc
    19501 agcctttgat atgggagtaa acttcattga cacagcggag ctttacagaa catatgatta
    19561 tataagaaaa gctgcagagg gaagaagaaa ggatataata atatcaacaa agtgctatgc
    19621 ctacaccagg gaaggtgctg aaaaaagcct tagaaaagcc ctggatgaat taaatacgga
    19681 ttatatagat attttttccc ttcatgagca ggaaagcgag catacattaa aagggcatca
    19741 ggaagccatt gaatatttcg tcagggcaaa ggaaaagggg tatatcagga gtatcgggat
    19801 atctacccat gccattgccg cggtaagggc ttcattaaaa tacaaggata tagaggtgct
    19861 tcatccgata ttgaataaaa ggggtctggg gataattgac ggagacgtaa acggaatgct
    19921 tgaagcagca gctgaggcat acaaggcagg aaaaggcata tattcaatga agcctttagg
    19981 cggaggaaat atgataaagg acagcagcca atgctttgac tttgtacttt caaacgacaa
    20041 cctgcattca atagccgttg ggatgcagag ttatgaagag gtagcaagca acgtaatgat
    20101 ttttgaagga aaacaggtgc cggaagatat caggctgaag cttgagaaga aaaaaagaag
    20161 gctgctaatt gattactggt gcagcggctg cggaagctgc agcgggaaat gcgcacagaa
    20221 agctattagt gtggaaaagg gcaaggcagt ggtggattct tcgaaatgtg tgctttgcgg
    20281 gtattgcagc gcttattgcc ctgatttttg cattaaggtg gtataacaaa caagagatgc
    20341 aagatgcgag aaagcctggg tattccttac aggtgttgtg ttactcgtgc tttatgtaaa
    20401 aatatatgga ggagaatttt tatgagaatt atgggattgg atttggggga tgccactatc
    20461 ggtgtcgctg tcagtgacga gcttgggatg attgctcatg gcataaccac aataagaaga
    20521 aagagcatgt attatgacat tgattgttta aaaaacatta tttgtgagaa agatataaaa
    20581 agaattatag tcggattgcc caaaaatatg aataacacta tcgggccaag aggagaaaaa
    20641 accatagagt ttgtaagaat actggaggag aattttccgg acattgaagt ggagatgtgg
    20701 gatgaaaggc ttactacctg cgctgccgaa agaactctgc ttgaagctga tgtaagaagg
    20761 aaggacagaa agaaagtcat agataagctg gctgcagttt tgatattgca atgttacctg
    20821 gacaatatga gaaatgataa tccttgaaat attcaggttt tattgacaca gtattgtcag
    20881 tagtataaaa tagttttacg agaaagattg gagtgatttt tgaatgagtg atgaaatgga
    20941 cataattctt ctttacgatg aaaatgacaa tgagaccgaa tttgaagtgg tcgctacttt
    21001 ggaagtggat gaaaatgaat atgctatact gctgccgaga gacgatacaa gagaccccga
    21061 tgctgaggaa gtggacgagg catatgtatt gagaatagaa caggatgaaa atggagaaga
    21121 catactcata ggcatagatg atgaagaaga gcttaatacc gtaatagaag catatgagga
    21181 actggtaaga gaagaatcaa attaatggag gcttgcagtt catagatgcg ttccgtagtc
    21241 ttagggattc gatccctaag gcttttttgc tttttattgt attcagtcag tttgccaagc
    21301 accttgtaat tttcctgtta aggaattata atgaaaaaaa ggaaaatgtc gaaaaaataa
    21361 tcctgagatg cctgtgaggt ggtgacggac atgcgtcctg aattgataga atttaatgac
    21421 ggaataccgg tcagggcgtt tgttcgcagc ggtaaccagt acccttacca ctggcatgac
    21481 gcactggaga ttattcaggt tctcaaaggc tcggtcgata tcggtatggg tgacgatgac
    21541 ctggtgctga gggaaggcga tattgccgtc acgaacataa acgagattca ccgcatgacg
    21601 ggtgacggag aagatgaaat ccttttcctc catatcgaca gcggcttctg ccgcagcgta
    21661 ctgccgaacc actacatttt catctactgc tgttctcctt accacgaggc ccaggcacct
    21721 gaaaagtatg gtgtcctgaa ggaatatatc gcccgcctgg tcggtatgct tggttcaagt
    21781 ccgaatcgga tcggtcagga agcccttcgg aagcctctga tggaaatgct gagctatact
    21841 gcatatagct ttgacttcct gcgttggggc tttggtaccg agccctttga cgaaaagcgt
    21901 gtggcaaggc tgcgacagat ggcacagaat gcaaccggcg actcggaggt gaaaaagggc
    21961 ttgacgatac ttgccgccga gctgggtgtg agcctccagc atttgagcag cgacattaag
    22021 gaaaagttca gagtgccctt tcaggagctg ttgtgctaca gccggtgcga gcgcgcggca
    22081 aggctgctgc tcggcaccga catgcgcatt gtggagatcg cgctggactg cggcttctcc
    22141 gacgtcaaat acctcatcaa gtatttccgg cgcttcttcc acgccacgcc atcgggcttc
    22201 cggaagctcc accgtgcgga cagcgcggcc ctcgcccggc aaacgcaata cagcgatatg
    22261 tctctcggcc atgccgtccg gcttctgtcg gcggagaccg acggagatca catagaataa
    22321 ccgaatgaac atgcccgaca cttaacccgc caaacggccg gttaattttt ttgaaaaaaa
    22381 cctgattttt tctctccgag cttaaaagtg tcaatgcttg atttgcaaaa accatataca
    22441 atgggatttg tcataggaca ctacgaccct acggttaaaa tcagggtcgc aaaatttcat
    22501 agattggagg acgagaacaa tgaaacactt gtttcggaaa acaacagctc tgttcctgac
    22561 gctctgcatg atcgtcggca tgacgggctt cccgataaca gcttacgcgg aggggcttga
    22621 cagcggtgac ggcggcgcgg ctgacaaagc tgctccgaaa atcggatttt cggtgacgga
    22681 cagcgtgtac ggcgtaaccg aactcaaaga ggaaaaactg tctatgctgt tcagcccgat
    22741 gtctgccgtg gaatgggtgg gcatcggcga tgaaaacagc ccttatcagg tatcgtcggc
    22801 ggagcacctt gcggatattc ccaatcaggg aatgggcgct cattacatcc agacagcgga
    22861 catcaacctg acaggcatca actggacacc catcggggat aacagcagtt ttgacagtac
    22921 cactcaattc accggcacct atgatggcaa taacaaaacc atatccaatc tgaccatcaa
    22981 caacactgag ctatattatg ttggcctgtt cggctatgcg ggcagcggcg gaacgctgga
    23041 aaatattatt cttgagaatg tcagcatcaa ttcttccaga gatagtgcct tcgtcggtgg
    23101 actgaccggc caaaattgcg gaggaacgat taaggatagc tgcgtttccg gcagtataac
    23161 ggctaccgga gatagcgccc gcgcaggcgg attgattggc gatagttatt taggtggaac
    23221 cgttgagaat agcagcagtt cctgcgccgt aaccggcggc gaaggtgtca gcaatactat
    23281 aggcggactg gtgggtcgtc aacagggagg gagtatcaga aacagctata gtaccggcaa
    23341 tgtgactgga acatattatg taggcgggct ggtcggttat ttgctgggtt cgggaaccat
    23401 tgaacacggt tacagcaccg gcaatgtgac tggaacgcca tctgatggaa caatcggcgg
    23461 gctggtcggt actgtcaaca acggaactat tactaatagc tgcagtacag gtaacgtaaa
    23521 tggaacaaat aatgctagta actatggtat aggcggactg gtgggctatt tttatggaag
    23581 cggaggcatt gccaacagct acagcaccgg cagcgtatcg ctgttaaaca gcggctctga
    23641 tacaaatcag ggaggcttta ttgggcttat tcaaagtacc agcgcaacta ttgcatactg
    23701 ctacagcacg ggcgacgtaa cacctggcga tggcagcgtc ggaggcttta tcggcttagt
    23761 tagagacaat cccaccgtca caagctcata ttaccttgat acagcggcag cttcctccgc
    23821 aagagggacg gctttatcag cgacggcgct gaaacagcgg gagagctttg tggactggga
    23881 ctttgatacc gactggaaga tcggcgaggg cgtatccagc cccaccctgc gctggcagcc
    23941 gtggacgacg gacgagaaaa tcaccgcagc ccatgacgct cttgattggg atgctatcag
    24001 gggcgcgaac accctggaaa gcgacgtaac cgcagacctt gtgcttaatg catcgggcgc
    24061 gcagggcgcg agcatttcat ggagcgccgc tcccgcaggc tttatcaaca ccgaaaccgg
    24121 cgaggtcaca acgcccctgt actcgcaggg cgatcagacg gtcacgctga ccgccgccat
    24181 cagccatgcg gacggcggta cgccgcagac aaaggatttt accctgaccg tcaaaaatgt
    24241 gcccgttaca tgggtgggca gcggcgatgc agacagtcct tatcaggtat cttccgcggc
    24301 gcaccttgac gaaatccgca taaagggctt gggagcacac tacatccaga cggcgaatat
    24361 tgacctgaca agcatcaact ggacgcccat cggggataac gtggatcaat ttaccggagc
    24421 atacaacggc aatgggtata ccatagctaa cctgaccatc aacgacgacg ggcaaaacta
    24481 tgtcggtctg ttcggatctg tcggtacggg aggaactctg gaaaatatca ggctcacagg
    24541 cgtaaatatt agttcaatca catggggagc ctatgtaggc ggtgtgacgg gcgtgaatca
    24601 gggaaccgtc acaaacagca gcagcgaggg cagtgtctcc ggaggcgata gcgcaaatgt
    24661 gggcggcttg attggcaacg gcggcggcgt tatcacatat aactacagcg ccgccaacgt
    24721 caccggcggc aataacgcca atgtcggcgg actgctcgga agaagctatg tcagtacctc
    24781 cgacaactat agcacgggaa ccatcaccgg tggggacaat gcaagagttg gcggcctgat
    24841 tggcgacgtc gacgacggaa ccgtcaccaa cagctacagc accggcagtg catccggcgg
    24901 aacgggggcc tctttgggcg gtctgattgg cggcggcaac gccaccgtca acgacagcta
    24961 ttacctcgat accgcaggca cctcagcagg cggcgaccct ttgaccgctg tgcaaatgaa
    25021 gcaatgggac agctttgagg gctggaattt caacaccatc tggaaaatcg gtcagaacat
    25081 atcctatccc actctacaat ggcagacatg gacagccgac gaggaaatca ccgcggccgt
    25141 cgccgccctt gcctggaccg atatacaagg aacaaacagc gcggaaaatg acgtaacctc
    25201 ggctcttgtg cttattatat cgggcgggcg cgacacggta atctcatgga gtgccgatcc
    25261 cacgggctat atcaatacca gcaacggcac actatcaaag ccctacaccg acgaggtaat
    25321 cgtcaccctg acggctaccg tcagcaaagg cagcggaata ccgcagacaa agcctttcag
    25381 cctgaccatc aagccgctga cgccggatga agccatcacg ctggataagg ctgccctgac
    25441 atgggctgtc atcaaaggta caaacagcac ggaggatcat gtgacggctg atcttgaact
    25501 tcctgatacc ggaaccagcg gcaccacggc aatcacatgg agcgccgatc cggtcggctg
    25561 catagacatt tctgacggaa aagttacatt ccccgatgac ggtaacaagg ccgtcactct
    25621 gacggctgcc atcaccaggg acggcggtac ggcgcagaca aaagacttta ccctgaccat
    25681 cattgcgcat tatcctgtga cattcactgc aggcacaggc ggaagcatca ccaacggcac
    25741 cagcggcagc tatgcggcag gtacggttat cgaaatatcc gccgcgcctg acagcggcta
    25801 tcagttcgat aaatggatta ccggcagcgg cggcagcttt ggtaacgctg cttccgccag
    25861 caccacattt acagtacccg caaacgccgt aaccgttaca gccactttta cacgcagcag
    25921 cggcgacaga gacgacagag gcaataaaaa cgcaccaaaa gcggcggaac cggaatacaa
    25981 ggcggaggta aagaacaaag acggcgagga agctgtgctt actgtcaccg ttgacaaaaa
    26041 cggcggaact gcgtccgtgg acgcaggctt acaaagcttt gatatagacg gatcggtaat
    26101 caccataccc accgtctccg acattgaggc ttattcagtc agcataccga tgacgagcct
    26161 gtcaacggct gatgcacagg gcacgctgac cgtaaacaca gataagggca gcatcactat
    26221 tccgtccaat atgctaacgg gtataccggg gattagcgga agcaaggcgc aaatttccat
    26281 cggtcaggga gataaaaata tcctgcctga cgcggtgaag gccgccgtcg gcgaccgtcc
    26341 gattatccgg ttaacggtct ccatcgacgg aaagcagaca gactggagta acccaaacac
    26401 tccggttact gtctgcatcc cttatacccc ggctgcggca gagcttgcta accacgaaag
    26461 cattgtagta tggtacattg acggaagcgg aaatgcggtt tccgtaccca acgggcgcta
    26521 cgacccggcc accggcacag tgacctttaa cactacgcat ttcagtcatt atgcgattgt
    26581 atatgtacat aagaccttca gtgacctctg cagtgcggaa tgggcaagaa aacccgttga
    26641 ggtaatggct tccaagggta tcatcaatgg aacagggaat gataccttct cacccgcagc
    26701 cagcatcacc agggccgatt atctggtact gctggtcaag acactggggc ttgcagcgga
    26761 atttgacagc aattttgatg atgtagataa gagtgcctac tattacgatg ccattggcat
    26821 tgcaaaaaag ctgggcattg tcgccggcag cggaaataac cggttcaatc cgcgggaaag
    26881 catttcccga caggatatga tggtgatgac agccagagca ctggaaagat tcaaaggatt
    26941 gaaggttttg gataccaacg gtgtgccgga gaaattcgcc gacaaggaag acatcgccga
    27001 gtatgccgta aacagtatgt ctgctcttat cacggaagga ttaatcgcag gcaccggaaa
    27061 taagctgaat ccctgtgcat atgccacacg ggccgaagcg gcggtattcc tatataatat
    27121 ttacagcaat tacccgtaag ctctttcaag gcttgcccgt cttatgcaaa gcaacgaacc
    27181 cgcaggattt tttcctgggg gttttgtttt atatttttat tttctaaatg atattgacaa
    27241 tcaatatcag taatgctatt attaaatcaa tgggaatgag aatcaatatc aaatagaagg
    27301 agatgttgaa tatgacactg gataaggttg cgagaggaaa taaggttaga attaccaaga
    27361 taaataatag ggatatgaaa actcaggcaa taaggattgg tttatatgaa ggggcagact
    27421 ttctttgcag cgagaggctt gcgggagggc caataatact gcggagcagg atgcaggagg
    27481 ttgccatagg cagaagactg gcagagggca tagaagtaga aaaaatataa atgtaaaaac
    27541 ttgaagggat aaggtagtat gagtggttgt aataattgca ataaaaattg cattaaagat
    27601 ataaaggata aaaagctggt tcttgccggc aatccgaatt caggcaaatc ggtttttttc
    27661 aatcatttga ccggaatata cgttgatgtc tcaaatttcc cggggacaac ggttgatata
    27721 agcacaggca aatacaaggg ctacacggta gcggacacac cgggggttta tggagtttct
    27781 tcctttaatg aagaagaaac ggttgccagg gatgtaatac ttgaagctga tattatactg
    27841 aatgtggttg acagtgttca tccgggcagg gacttgttcc ttacaaaaca gctgatagat
    27901 atgggaaaac cggtattagt ggcgttgaat atgattgacg aattgcaaaa gaacggcatg
    27961 gatattgatg ttaagctgct ggaagaactg ctgggagtcc cggtttttcc aacttctgct
    28021 gttaaaaata ttggagtaac ggacgtgttg atgtcattgg aacaggcaag gcaagggaat
    28081 agagatcccg ggcttacaga aatgatgaag cagttcgggg atacatgcag cgaggcggaa
    28141 gctttaatga tacttgagga tgacgaggtt gtcgctgcca gatacggcat cgaaacctcc
    28201 ggcttgcgtg atgaaattta tatggaaagg cgcagggcgg ttgaagaaat agtaaataaa
    28261 gtactgaagg aaacaagtaa gggagcttcc ttcggaattc tgctgggcag gtggatgctg
    28321 aggcctgtta ccggaattcc gatgctttta ctgactttat atgccatgta taaaataatt
    28381 gggattttcg tagcgggaac ggtcgtaggc tttaccgagg aattaatcat ggaaggcatg
    28441 tacaagccct ttattatatc agtactgtca ctttttatca gtgaaggcag ctttataggc
    28501 aatatgctga tcggagaatt cggaatactt acaatggtgc ctgtatatat aataggcctg
    28561 ttattgcccc ttatcattgg tttctatgtc ttaatggcag ttctggagga cagcggttac
    28621 ctgccgagaa ttgctgcact ggcggacagg atgcttatat cactcggact taacggtaag
    28681 gcaattatac ctattatact tggctttggt tgtgttacaa tggcaaccat gactacaagg
    28741 atattgggta caaagaggga aaagattatc gctacggtac ttttggggct tgcgatacca
    28801 tgctccgcac agcttggggt aataatgggg ctggtttcag gattggatgt attctatata
    28861 atcacgtata tcacagttat tacttcaata tttgtgcttg ccggtacggt gctgaataag
    28921 ctgatgcccg gagaaagctc ccatcttctt atagaccttc ccacaatcag gctgccgatg
    28981 cccggaaatg ttgtgaagaa gacttttcag aagacggtga tgttcctgaa ggaggcagta
    29041 ccgctgttca tatacggggc attgcttata agtgttttgg agcaaacagg tgcattgtca
    29101 tatatacagc agtggatgac accaataacg gaaggcttgc taaagcttcc gcctgaaacg
    29161 gcaacggcat ttattatggg cattatcagg agggatttcg gtgccgcagg gctcacttca
    29221 atagcaatga cacagcagca gctgcttgtt tctctgatag ttatgacgct gtttgtgccg
    29281 tgcatagcag caataatggt tatttttaag gaaagaggca caagggatgc tctggcaata
    29341 tggctgggaa gctttgcggc agcattcatc acgggaggga tagttgcatg gttgatatga
    29401 agaatggttt aaaatgcggc aagtgcggat atacattgaa ggagcagagt aaatgcccca
    29461 gatgcggaac tgttgcgcag tgcagcctgt catgcccggg caattgcctg aagtgctttg
    29521 cagaggcggc aaaagccaat aaacaaaaat tagttgacaa aaatgcagaa taaataataa
    29581 tattaaatgt aaggaatatt gtacaaaaaa atactaatat atcaaaatat aggtgtgcaa
    29641 gatgtgactt tttaaacaag atataaagga agttgaaagc tttgatacga gagtttgagt
    29701 acttaaggga aaagcttaag gagaaagggt ttaaactgac ccctcaaaga agagctatac
    29761 ttgacatcat attggagact gaaggcaagc acttaagcgc ggaagaaatc tatgacctgg
    29821 ttaaggtaaa gtgccccgat ataggcctgg ctacagtgta tagaaccatg cagatgtttg
    29881 aagaggtagg gcttgtatat aaacataatt tcgatgaggt aagaagcagg tatgagatta
    29941 atcaccacaa tgagtaccac cagcatcatc atctcatatg tgtcgggtgc agcaaggtaa
    30001 tagaggttga ggaggatttg ctggaacaat tggagtccgg tatagaaaag aaatataatt
    30061 ttaagataag taatcataac gttaagtttt tcggatactg tgaaaagtgt agaaagtgac
    30121 ttgcggtatt gcatagcata tgcaatacca ttttaattgt acaggaagcc ctgcccgcat
    30181 attctgatag taataaatga tattctattg actaataata gctaatagcc taaagtaaga
    30241 tgtttggcac ggaacatata gtatgtttag tgtgaaatat attaagaacg gaaaaaatga
    30301 aaggattgat aaagaaagtg gcaaaaagta aggcaaaatt aaaattaata cccctcggag
    30361 ggctgaatga gatcggaaaa aacatgacag ccgtcgagta caaggatacc atagtcataa
    30421 tcgactgtgg gctgatgttt ccggatgagg agatgcttgg aattgatctt gtaatacctg
    30481 atgtaacata tctggtgaaa aacaaggaaa aggtcaaagc catactgctt actcacggac
    30541 atgaggatca tataggtgct ttgccctatg tgcttcctca gataaatgtt cccgtatatg
    30601 gtacaaagct taccctggga ctggtggaga ataagcttaa ggaattcgga cttctttcat
    30661 tggttgagcg gataattgtc aagccaaagg acatggtgaa attcggcgaa ctggaggtag
    30721 agtttataaa aacaagccac agtatagctg attcagttgc cctagcaatt cataccccgg
    30781 ttggcattat agttcactcg ggggatttca aaatcgatta tcagccgata gacggtgagc
    30841 tgattgattt gcacagattt gcggaacttg gaaaacaagg agtgctggtg cttatgggtg
    30901 acagcacaaa cgttgagcgc ccgggatata caatgtctga aagcactgtg ggagctacct
    30961 tcatggaact tttcagagat gcaggcggaa gagtaatatt ggctaccttt gcatcaaaca
    31021 ttcacaggat acagcaggca ataaatgcgg ctgcaatgtt caacaggaag gtggcggttg
    31081 tcggcaggag catggtcaat gtaataggag tagctacaga gttgggctat attaatatcc
    31141 ccgagggagt tatgatagaa gttgaccata tagacaagta tccgaatgac agaataatga
    31201 tacttaccac agggagccag ggagagcctt tgtcagccct ggcaaggatg tccactgcag
    31261 aacaccggaa ggtaacaata ataccgggag acagagttgt tatttcagca aatccgatac
    31321 ccggaaatga aaagcttgta tccagagtta taaatcaact ctttaagaag ggtgcagatg
    31381 ttatatatga gtctctggtg gatattcatg tttcaggcca tgcataccag gaagagctga
    31441 aactgcttca agccctgata aaaccaaagt attttgtccc ggtgcatggt gaatacaggc
    31501 atcttacgca gcacgcccaa ttggcggaaa aaatgggtgt gaacaaggag aatattttta
    31561 ttgccgatat cggaacagtt ctggaattta ctaaagacag cggcaggaca gcgggaaccg
    31621 ttgttgccgg gaaggtactt gtagacggcc ttggagttgg agatgtggga aacatagttc
    31681 tgagggacag aaagcatctt tcccaggacg ggctgatagt tgtggtggta accataagca
    31741 aggagacagg acaggtagtg gcaggccccg atataatatc aagaggcttt gtatatgtcc
    31801 gtgagtctga agacttaatg gaagaggcaa ggggagtaat aaaggatgca ctggcaaaat
    31861 gcgaagaaaa acagataaca gaatggggaa ccatcaaatc cgaggtcagg gatagcctcc
    31921 gggagttcat atatgaaaga accaagagaa atcccatgat tcttccaatt attatggaga
    31981 tttagactgt tatggataaa atatgtatag tagttaatcc tgtttcagca aacaaaacaa
    32041 cagccaggga atggccctta tttgaaaagg tattgtcgga tgcaggtttt gaatttgaag
    32101 ctgtactgac tgaatacccg ggacatgctg ccgaactgac aaggaaggca ttaaagcttg
    32161 gctataatac aattatgtct gtcggcgggg atggcacaat gaatgaagtg gtcaacggtt
    32221 tttttgaaaa tgaccggctc attgatgaga attcaaggct tgtagtattt tcaagaggaa
    32281 cgggctgtga ctttgtacgg agccttggaa ttaatagaaa tatagaggat ttattggcta
    32341 ttctgaaaag gaatcgggaa aaacaaatcg atgtgggacg tttaagcctt gtccgaactg
    32401 aagggaatat gcctgcaagg tattttttga atgtcgcgga cacgggcata ggcgccgaga
    32461 cagcaaacag agtaaatatg cattcaaaga ggctttcggg ctttctttcc tttatgcttg
    32521 gaacattaag cactgtggtt ttgtacaaga atcaaaattt tgaagttgtt atagatgata
    32581 agatacattt gaacggaatt atgaacagtg taatagtggc aaacgggaag tttttcggcg
    32641 gcggaatgat ggtggccccg gatgcggtgt tggacgacgg agtatttgac ataatcattc
    32701 tgggaaactt aagcaagtat gatattataa aaagctttcc cttgatatat aaagggaagc
    32761 atatgaacca cccaaagctt aagtattgca gaggcagcaa agtcaaggta acaagcggcg
    32821 gcaaaggcct tattgaagcg gatggagaaa tccccggcag ggctgacgct gtttttgagc
    32881 taatgcccaa ggcattgaaa atccttattt aacctaattt atatattgga gaagatacaa
    32941 tggaaaagac gataagagaa gatataagaa atgtagcaat aatagcacat gtggatcatg
    33001 gcaagactac cttggtggac gggcttttaa ggcagagcgg tactttcagg gcaaatgaaa
    33061 acgtcatgga aagagtaatg gattcaaatg acctggaaag agagcggggc ataaccatac
    33121 tggcaaaaaa tactgcagta atatataaca atatcaagat aaacatagtt gatactccgg
    33181 ggcacgcaga tttcggcgga gaggtggaaa gggtcctgaa aatggtaaac agtgtgctgc
    33241 tcattgtgga tgccgctgag gggccgatgc cgcagacgcg ttttgtactg aagaaggctc
    33301 tggagcttca actgaagcct attgtggtaa taaacaagat agacaggccg gattcaagga
    33361 ttgaggaagt gattgacgaa attatcaatt tgttcattga acttggcgcg gatgataatc
    33421 agctggaatt cccgattgta tacgtttctg caaagcaggg agtggcaaag ctggatataa
    33481 aggatgagtc tcaggatttg aggccccttt ttgatacaat aatatcctat gtcgaacctc
    33541 ccaaaggtga tgcggaaggc ccttttcaga tgcttgtcac taccatagaa tatgatgaat
    33601 atgtcggcag gattgctatc ggcaggatcg aaagaggcag gataatctcc ggaatgagcg
    33661 ctgctctttg caaaagggac ggagtgctgc aaagtgtaaa gctgagcagg atttacagtt
    33721 tcatggggct caagaggaag gaagttgaag aggcatttct gggagacata attgccgttt
    33781 caggcataga cgatataaat atcggagaga caatttgcga tattcagaat ccggaggcaa
    33841 taccctttgt caatattgat gaaccaacaa tttctatgac ctttagcgta aataacagtc
    33901 cttttgcagg ccgcgaaggg gattatgtca cttcaaggca tttaagggca agattgttta
    33961 aggaattgga aacaaatgtg gcaatgaggc tcacggaaac ggattccccc gacactttcg
    34021 aggtggcggg aagaggagaa ctgcaccttt caatactgat agagacaatg agaaggcagg
    34081 gctatgagtt ccaggtatca aagccgaatg tcattatgag gaatatcaac ggtgcagcct
    34141 gtgagcccat agagcatctt attattgaaa tacccgaaga attcatggga gtagtaatgg
    34201 acagattagg ccccaggaag gctgagatga tgaatatggg caacagcagc gacggttatc
    34261 tgcgccttga attcaagata ccggcaaggg ggcttattgg ctacaggtcc cagtttatga
    34321 ccgatacaaa gggaaacgga accatgcacc atgttttcta cggctacgag gaatacaagg
    34381 gcgaaattcc cgacaggccc aggggctccc ttattgcctt tgaaaacggc gagactacgg
    34441 cttacggact ctataacgct caggaaaggg gatatttgtt ccttggaccg ggagtgcagg
    34501 tatatgaagg aatgatagtc ggagagaatg caaaatccga tgatatagag gtaaatgtct
    34561 gcaagaagaa gcatgtgtca aatatgaggg cttccgggtc ggatgaagca ctgaggctga
    34621 ctccccataa ggagatgagc ctggaggaat gccttgaatt cattgcggcg gatgagcttg
    34681 tggaagtaac tccaaaatca ataaggcttc ggaagaggat attggacagc agcctcaggg
    34741 caaaagccag gaataagtaa aaaataggac acaaagacgg ggacatgggg acgtttctcc
    34801 tgtcccgctg aaagcgggac aggagaaacg tccccatgtc cccgtccttc tttgggtgca
    34861 tttttattct gttaatcttg tcaaaagatg ttacacatta tataataaag tcagcgattt
    34921 ttgaaagaag tgtttataat gaaagcgaaa ctcaaattta acatattcag gtttttagtt
    34981 ttctgttctc ttcttgccct tgttctcgct tatgtatatt acaataacgg aatggaggca
    35041 gtaagcggag aaggatctga ttttaaagaa attgtcatac caaaaggcag tacaatcaga
    35101 tatatatcaa aaattctgaa agaagaggat ttgataaagg acagcttaat ttttgagttg
    35161 tattgtaaga ttaatgaaaa agcagataaa atcaaggctg gcagatacag catcagcagt
    35221 tcaatgaagg tttcggagat agttgatgta ctggtatccg ggaaggcact tatagatacc
    35281 gtaaagttca caataccaga aggctacaat cttgctcaga tagctgaaag gattagcagt
    35341 ctcggggttg taagtcctga gagcatacag gctgctttga aagcggaggg atatgaatat
    35401 gagttcatag aacagatccc tgacagggaa aataaactgg aaggctatct atttcccgat
    35461 acctatgaaa tatacaggga tactaccgct gaggccatta tcaaaaagct tctgggacgc
    35521 ttcgacgata tatttacgga gaaattcaga agcagggcgg aagaacttaa tatgagcatt
    35581 gatcaggtgg taaccctggc gtctataata gaaagggaag ctaagctgga ttcggaaaga
    35641 aagactatat caggagtatt ccacaacagg ctgaaaaaga aaatgctgct tcaaagctgc
    35701 gccaccgtac agtatctgtt aaaggaaccg aaagaagaac tgctgtatga ggacttggag
    35761 attgattcac cctacaatac atacaagtat gcaggcttgc ctcccggacc cattgcatct
    35821 ccgggccttg cggcaataga agccgctctt taccctgaaa atacggattt cttgtatttc
    35881 tttgcattgg aagacggttc ccatgtattc acgaaaacat acaacgagca tatcaatact
    35941 cagaataaat atagaaagta attcaaaaac tatagaagaa taatgcgaca taatgagaat
    36001 tgcaaatacc gacgtgggga agctatattc gacgaaacct tccacctgac cacggaacgg
    36061 gaatacccct ggcaaaataa ccagacgggc ccgcagagat gggacccgcg ccagtgattt
    36121 tgctgacttt ggaaggtgag gagaacatac ttcccctaca gtcggagtac tttacaaacc
    36181 tatatattac gcattatttt ttttatgcga ggtgatactg tggcaaatat cagccatgat
    36241 tatatcgaca gcttcctgct tggcatgata cccgggcgaa gcggaatact aaaggaaatg
    36301 gaagagtatg caagggagaa ccatgtgccc attattactc ccaatattgc aagcttgctg
    36361 gcagtgctta taaagactgc aaaggtgaaa tccatactgg aaatcggtac ggctatagga
    36421 tattccgcaa tacatatggg gctgtgtgcc ggtaaagatt tctcaataac tacaattgaa
    36481 agaaatgaag aatatgctgc attggcagca gattttatca ggaaggccgg catgggggat
    36541 aatatcagga taataaccgg agctgcggaa gaaatgctga aagacattcc aggcagcttt
    36601 gatatgatat ttgttgatgc ggcgaaagga cagtatatgg attttatgaa aaaaagcatc
    36661 ggcaagctca ggattggcgg cctgtatgta tgtgacaacg tgcttttcag gggaatggtg
    36721 gctgaaagaa gcttgctgat aagaaggaaa atcactattg taaaaaggct caagaagttt
    36781 ctgagcttta ttacttcctg tgaaagcctg gagactgctg ttattcccat gggtgacggt
    36841 atatccgtat gctgcaagct taaggaggtt gagttcattg aataaaccgg aattacttgc
    36901 tccggcggga aacctggaaa aattaaaaat ggcgattatt tacggtgcgg atgcggttta
    36961 tatcggcggt gaaaaatatg gcctcagagc cgctgcggga aatttctccc ttgatgaaat
    37021 caaagaaggt gctgcgtttg cccatgataa ctgcagaaaa gtatatgtta cagtaaacat
    37081 tattccgcat aatgaagatt tggaagggct ggaggaatat ttaacaagcc tgggagatgc
    37141 gggagccgat gcacttatcg tatctgaccc cagtgtaata atgacagcca gggaagtatt
    37201 gcctgatatg cagctgcatt taagcactca agccagtaat acgaattata tgagcgcaag
    37261 gttctggcac agccagggaa taaaaagaat agttctggca agggaattga gctttgaaga
    37321 gataagggaa actatagaaa agtctccaaa aagcttggaa tatgaagtat ttgtacacgg
    37381 agcaatgtgc atttcatact caggcagatg tttgctcagc aattatatga cgggcagaga
    37441 tgcaaacaga ggcgaatgtg cacagccttg caggtataag tactatcttg tggaagagaa
    37501 acgccccgga cagtatatgc cggttgacga agacgaaaga ggaacttaca tattcaattc
    37561 aagagattta tgcatgatag aatttattcc tgaattggcg gaatcgggag taaaaagcct
    37621 gaaaattgag gggcgtatga aaagctcata ttatgtggca tctgtagtaa gagcatacag
    37681 aatggccctg gacagctatt tttcgaatcc cgggaactat gaattcagga aagaatggct
    37741 ggaggagctg tcaaaggcca gccacaggga attcggcaca ggcttttttt tcggcaagcc
    37801 cgattcacgc gggcagattt atgaatccgg cgcttatgtg agggattatg cctttgtagg
    37861 tttggtattg gattatgaca gcaagacagg catcgcaacg gtagagcaga ggaataaaat
    37921 gctggtggga gatgaaattg aggtaatagg gccccgcaga gagatgttta ctcaaaaaat
    37981 tgagaagatg tggaacgatg agggagttga aatattatct gctccgcacc cgcagcagat
    38041 tgtaaccatc aaaatggaga ggcccgtgga aaaacatgat atattgagaa gggagaggaa
    38101 agacggtgag tgaagaaaag catagaaagc cgcttgtaat cggaatagca ggaggaaccg
    38161 ggtcaggcaa gagcacggtt tcaaaggcta ttacggacag cataccgggg aaaaaagtag
    38221 ttatcattga acaggattgc tattacaagg accaaagcca tcttccgccg gaggaacgag
    38281 ttaacgtgaa ctatgatcat ccccttgcct ttgataacga gttgctgatt gctcatatgt
    38341 taaagttgat aagcggacaa gctattgata agcctcagta tgattttacc gtgcacaaca
    38401 ggaaaaagca tacattgaga gttgaaccca aagatgtaat acttctggaa ggcattctta
    38461 ttctggattc tccgaggctc agagagttaa tggatattaa gatttttgta gatacggatg
    38521 ctgatgtcag ggttataaga agaattgtaa gagatatgag ggaaaggggc agatctcttg
    38581 agtcagtgat tacccaatac ctgaatgttg taaagcctgc ccacaatgag ttctgtgagc
    38641 ctacaaaaag atacgcagac ataattatcc ccgaaggggg ctacaataag gtggcaatag
    38701 atataatggt gacaaagatt aaatcgatta ttgacggcgg ctgtgattca aactgaagtg
    38761 aataacctcc cacaatctga caatactaag attgagggag gttttgttat ggccggaatt
    38821 atgaagttct taagaaaaaa agctgtgccg gagtgcagga aggaagagga aaacaggagt
    38881 atggagcttc ttacactgcc aggtacaagg ctttacagga aaaacaacta tattcttatt
    38941 cataagaaaa gacttcaggt aataggtctt ttatttattg ctgcatgcgg gcttctcctg
    39001 tgcaggcttt tctacatcca ggtatatatg ggtaaacact acagtgagag ggctgtgcgg
    39061 caaaggatgg ttcatatacc catttcctca aacaggggta ttatttatga caggaatatg
    39121 atacctatta ccgatagaga agtaaaaaaa gtggttattg catatccgga ttacgtatat
    39181 gataagaaaa ccgctgttga gaccatttcc aaagcatgcg gcattaaaat ggaagatgtt
    39241 gaaaaaaaga tgggttcggc agatacggcg gaattcatat gtgaaacaga gcagaatgag
    39301 tatttgtcat tgattgaaag cggcaggata cgcggtgtca ttgccgtgga aaaaaggctg
    39361 agatacgctg atgacaatat cgcaaggcat gtagtgggct acataggaaa aacggacaaa
    39421 acaggccaaa tgggtattga aaagagtatg aacagttacc tggaaagcag aggcagcgac
    39481 agtatcgcag ctgtagtgga cagcggaaag aatatcatac gggggctggg cttcagaaag
    39541 gttgaggcgg caggagacgg tgtaagctac agccttaaac ttacccttga ctatcatatt
    39601 cagaatataa ttgaaaccgc aatggaaaaa aacgacgttg aaggctccat agtggtaatg
    39661 gacgtaaaaa atggcgatat actgggaatg gcaagcaaac ctgattttga tcagggcaac
    39721 gtggacaaat acctgaaaag cagcggaaac gagctgatta ataaatcaat ttggcaattc
    39781 gaccttggtt caatatttaa aaccgtggtg gctgcagctg ccattgaagg agggtatgtg
    39841 aacccggaag aaaaatacaa atgccggggc tacgcagatg tgggcagcag caggataaaa
    39901 tgctccaccc ataaaaccca tgaagacata gagattaata tgaaggaggc ttttgccctg
    39961 tcctgcaaca gcacttttat acagatcggt acagccacag gggctgaaaa catactgaaa
    40021 atggccggaa ggctgggcct cgggcaaaaa ctgtgcttta ccattccgga agaaaaggca
    40081 ggttacatac cggaagcttt tgaggacggt ataggaaata tatccatcgg acaggggaag
    40141 atacaggtta caccccttca ggtaacatgt atgatggctg ccatagcaaa taacggcatc
    40201 aggaatgacc ctcagataat agattccctt attacggaaa agggcttgac agtcaaaaag
    40261 cttgaaaggt caaggcccca gattgtgctt aatcccataa cctctaagct gttaaaggat
    40321 atgctccatg aagtcacggt aacaggtacg ggaaagcagg caaatatgga tgaatacgga
    40381 ggctgctgcg gcaagaccag ctcagctcag acgggaatta aagaaggaga ggttgttcac
    40441 ggctggtttg caggctttat tccctatgaa aagccaaagt atgccataac ggtattcata
    40501 tataacgggc aatcgggggg cggtgcggca gctccgttgt ttaaggaggc agcttccaaa
    40561 attttgactg cagttaaacg atagattcac ataaaatttt ttatgttatg tatttataca
    40621 ggcacacatt cggaatgtaa atcatataat ttcaataggc gaagttttcc attgtatgaa
    40681 ccttgtacgt ggcagacttc tttaaaattc ggaggtgtgc ttctttgctg ctctttttag
    40741 cctctcttat tgttttcaca ataaaggacc tatgcctgtt attcggttac attcagaatg
    40801 tgaactcatt tccgcagccc cttacaactg aagaagagca gtattatctt gaagagtaca
    40861 aaagaggtaa tgaagatgca aaaaacatac tggtagagcg gaacctcagg cttgtggcgc
    40921 acatagtgaa aaagtatgta aatacgggaa gagaaattga tgatttgata tccataggaa
    40981 ccataggcct cattaaagca ataataactt ttgatgagag caaggggaca aggcttgcga
    41041 cctatgctgc aagatgtatt gaaaatgaga tcctgatgac tataagagcc tcaaaaaaaa
    41101 caaaaaccga agtgtctctc catgatccga taggaattga caaggaaggc aacgagataa
    41161 gtttaataga catattggga agtgattccg atataatact tgatgaagtg gagcttaaga
    41221 ttcagattaa gaaactgtat caaagaatgg atacagcttt gaaaaaaaga gaaaggctgg
    41281 ttattgaact gaggtatggg ctgcttaacg gaatcagcaa gactcagagg gaaatagccg
    41341 atatgcttgg aatttcaagg tcatatgtgt caagaatcga aaatagggca atcaaaaagc
    41401 tgcataaaga gctttacaat gaaaacgggt aagaatagaa tagaggtacg gggtcttaag
    41461 gctgctgtat acatgcagca ttcataaaaa atgtacaatt gaggtaagat atgaaaaaca
    41521 ggcttaaacc ggatatttat ttgaattcgg tatatgaatt ggatacagct tcattgaagg
    41581 aacatggaat aaatgcaatt atagtggata ttgacaatac tttggtttcg tggaatacaa
    41641 gagagcctga cgaaaaggcg aatgaattta tgtgccgatt gggttcggaa ggctttaaaa
    41701 tctgtatatt atcaaataat tcaagaaaaa gagtggagga attcaataaa tgcctgaacc
    41761 ttcctgcaat tcataaagca accaagccaa gcaagtttgc ctttagaaga gctatgaagc
    41821 ttatgaaatc caaacctgct gatacggcag taatagggga tcagcttttt acggatgtac
    41881 tgggaggtaa ccgcttgggg ctgttcacca tacttgtgtc accaataagt aataaggaat
    41941 tcatatggac aagaattgtc cggaagctgg aaaagcatgt tttgaggaaa tatcgtcaaa
    42001 caaataatat gcaatgataa atgaaataaa atcctgtgag tatttgcagg atttttattg
    42061 ttgtttgtag aatgatataa gttgaaataa attggtaaag gcttcattat tggtgaagcc
    42121 tttttttagg actaaaggtt tgccaggcgg atggaaagcc aggaagggtt attgattatg
    42181 cacggtttga ctgttatatc aagtagcgct tttactgctg cttctgtttt gctcgtaaag
    42241 cttttggaga ggaaggtcgg ggcgggcaaa ggcgacaaaa taggcttaag ctttgcggca
    42301 cctgtattta tactgttttt atctgtaaat ttgtatatga taaataagaa tgccgaagga
    42361 agctatttga tactgaatgt tctgcttctg acagctgtgg tttcttctta tcaggacata
    42421 aaatacaggg agatagagga tgaaattcat attgcggctt tgggcgctgg actagtgggc
    42481 cttttaataa agggctttca gccggcggat gcatttttgg ggctgatggc agggggcgga
    42541 acactcttat tgatagccat attgacaaaa ggcggaatgg gaggagctga cataaagctt
    42601 aatgctgttt acggtgcaat actgggattg aagctttcga tgctttcact tgtaattgca
    42661 tttgcagcgg gagcgttggc aagtctgctg ctgatattgt tcgggataaa gaagagaaag
    42721 gatacaatac cattttcccc tttcctttct attggcgctc tgaccgcgtt tgttttcggg
    42781 aaaagcataa tgggcatata tctcgggttc ttttagatat aattaataag gaggactggg
    42841 tatgtataaa ttaaaaaact atatggagga tttggtaatc aagcgaacgg atgaaatttt
    42901 aaagtccata gatgtctgca agtgtgataa atgcaagctg gatataatag cacttgcttt
    42961 gaacagcctt ccgtccaagt acgttgtatc tgctaaaggg gagctgtatg caaaggtcag
    43021 cgaattggaa cagcagtttg atatagatgt tgggactgca ataataaaag ctgcgtttct
    43081 tgtgaatgag aatcccaggc acaatgagga ataagtgcag gtgctattct gaagcagcat
    43141 ggatgtatga gaaaattgga tatactatta tatatggcag caagggcatt taaataaatt
    43201 gcttaaggag cgcaaaatga tacatttcgg accatcgggt aattcagatt ctttttatga
    43261 gcaaggctat aaaagctccc ttgacatgcc tgcctggctt agggatatgg gattggacgc
    43321 ttatgagtac tcctgcgcaa gaggtgtgag aataaagcct gatatggcgg aacggttggg
    43381 aaaggcagca agagaaaacg gaatcacttt gagcgtacat gctccgtact atataaatct
    43441 tgcatcagag gagcccgaag ctgttaagaa atccatcaat tatattattg atacctcaat
    43501 cacagcagag ttgatgggag ctatgcgtgt cgttctccat cccggctctt tgggtaagct
    43561 gacaaggaat aaggcttttg aaaacatccg gagtaattta ttcagagtat tggaaataat
    43621 aaataagaaa ggaatcaagg tttccctttg tcccgagacc ctggggaaga ggaatcaaat
    43681 ggggacattg gatgagatac tggaactttg cctgctggat gaattactga taccgactgt
    43741 tgattttgca catcttcatg cgcttggttc aggcgcactc aggaaaaggg aggattatga
    43801 aaaggtattt gacaggattt tcactgttct tggggaaaag agaggcagat tgatgcacat
    43861 gcattacagc agggtagagt atacggaaaa gggcggagag aaaaagcact ggtcctatga
    43921 cgatgttgag tatggccctg aattcgagcc ccttgcagag tgccttatca aatttgggat
    43981 ttccggaaca attatcagcg agtcaagggg tactatggcg gaggatgcat tgaagataaa
    44041 aaaaatttac ggcagattgt caaaggaaaa cctgtacgct atggaaaaat tatgttaata
    44101 ttatattatt cgcagaagta ggagtcttca gtttaaataa atacatacac ggagggaaat
    44161 gattaatgat atcagcgagt gaatttagaa aaggtcttac ttttgaaatg gatgggcaaa
    44221 tatatacaat tgtagacttt caacatgtta agcccggcaa gggtgctgct tttgtaagaa
    44281 caaagataaa aaatatcata accggaggag taactgaaag gacatttaat cctacggata
    44341 agtatccaaa ggcgcatatt gagagaaaaa gcatgcagta tttgtatgaa gacggcggac
    44401 tttattattt tatggataat gaaacctatg agcagatacc tcttaaccat gagcaggtag
    44461 aggatgcaat tatgtatctg aagcccaatg atacagccaa tataaaattt tataaagggg
    44521 aggctttttc agtagagccg gagaactttg tggaaatgga agtaactcag actgaaccgg
    44581 gtttcagagg ggataccgct accggagcaa ctaagccggc tatcgtagaa acgggtgcaa
    44641 cagtctatgt tcctttgttt atcaatactg gtgacagaat cagaattgat acaagaacag
    44701 gggaatacat ggaaagagta taatcctgta tatagttgcc gatactggta agaatactga
    44761 gagcatataa aaaataagga ggagtacata tggaatacct atacgaaaga gtagcctatc
    44821 ttaaaggcct ggcggaaggg atgaaggtag acgagtcaac agatcagggc aagttaatca
    44881 ctaaaattat agatgtatta agtgattttg cagatgcagt aaatgaactg aatgaatcac
    44941 aaaatgagct ggatgaatat gttgaagcaa tagatgaaga cctttcagca ttagaggatg
    45001 acttgtgcta tgacgaagat gacgaagaag agtttgatga cgaggaggac tatgattaca
    45061 ttgaggttga gtgtcctcat tgcaaggaat ttgtctatct tgacgaagac cttgtagaaa
    45121 gcggtgagca ggtcatatgc cctaactgtc atgagccgat cgaatttgaa tacgagtgcg
    45181 gctgcggctg cgaccatgac caggaatagc cggcaaaaga agctgaatta taacccgatt
    45241 gaatccaagt attcaatcgg gttatttttt ttcccgcagt aattatttca aatcagttgt
    45301 catatctata gtacaaatta aaatttgtac aaaggacaga tgctttatga agaatgatat
    45361 aaaaatactt gaggtactgg aatatatgcc gaagcatata ggggcgatga tagcgaagct
    45421 cccggaaaaa atacttatgg atgcggaaga aataagattg aggctggcaa agcccttatg
    45481 cgtggcaggg agcaatgggg agctttttat ttctgcggac gggtacgcag tatccgaggc
    45541 agaagaggca tatcttgtaa gcgaggaaga cttgaagcgt gcacttcagc tgacatgcaa
    45601 tttttcattg tactcggtgg aggaagaact gagaaatggt tttataacgg taagcggcgg
    45661 ccacagggtg ggggtatgcg gccgggcagt agttgacagc ggcaaggtaa agaccatgaa
    45721 ggacataagc tatatgaatt tcagagtggc aaaacaggta ttaggcgcat cagacaaagt
    45781 atcgggctat ttggtacagc ctcccgacag catattcagc actcttatta tatcaccgcc
    45841 tcaatgcggc aagactacat tgctgagaga tttagtcaga aagctcagca gcggaagcag
    45901 agacataccc tttaagggca tgaagatttc tttggtggat gagcgctccg aaatagccgc
    45961 atgcagtatg ggcataccca gaaatgacgt agggataaga acagatgtac tggatgggtg
    46021 cccaaaggcg gaaggaatta ttatggtcat aaggtcaatg tctcctgaga tagtggctgc
    46081 cgatgaaata ggaagggcgg aggatgcaga tgccataacc gctgcagtca atgcgggggt
    46141 gaaagtcata actacaatac acggcagcaa tataaccgat tttttgaata agcaggatct
    46201 taaacgtatt caaaagggag tttttgaaag gtatattgtc ctgagcagga agaagggtgt
    46261 cggcactctt gaaactgtct tggatgggga attcaatgta ctgtatgaaa gaagcaaaag
    46321 catttgtaaa tggggggata gagttgatgc ttaagattat cggaggcctg ctggtgattt
    46381 ccgcaagcgg attattaggg atggtttatt catataaatt gtcattgcga tacagagagc
    46441 ttgcaaatct caggagatta atgcagatgc tggaaacaga agtgacatat ggggctacac
    46501 ccttgcctgc cgcacttttc agcgtatcaa aaaaggcgga aggcccgatt ggcagctttt
    46561 ttagctgtat ctcccggtgc ctttcggaca gaagctttta tactgtgaga gaagcatgga
    46621 acgattcggc tgaatcgatt cttactcagt cttcattgaa aatggcggac attgatctta
    46681 taaagagctt tggaagcata ttgggatgct ccgacaggga agaccagaaa aagcatttcc
    46741 agctttttta tcttcagctc agccatcaag aggctgcagc aacggaggaa ataaagagaa
    46801 gtgcaaaaat gtacagaagt ctcggttttt tattgggaat tgcagtattt gtagtattgg
    46861 cataaatacc ggcgaaaggg gcgataaaat ggatgttgat ttgatattca aaatagcggc
    46921 tataggtata gttacagcgg ttctgaacag agtcctgaac cagtcaggaa gggaagaaat
    46981 ggctatgatg actactttgg cgggagttgt agtagtgctt atgatggtaa taagccttat
    47041 tagcaatctt tttaatagtg taagaacaat gttccatttg tagggtgata ttatggatat
    47101 catgaaaatc gttatggtag gcataattgc ggcattgctt gcagtagtcc tgaaggagga
    47161 aaagcctgaa atggcatttg caataagcat tgtcaccgga ttggttatat ttgtatttgt
    47221 aataaccaag cttaactccg tcatgacggt gctgaagcat tttgcggcta aagccaatat
    47281 tgacgtatta tacttcacta caatacttaa ggttattgca atagcatata taacggaatt
    47341 cggcgcccag atatgcaggg atgccggaga gggtgccatt gccgcaaagg ttgaatttgc
    47401 ggggaaagtg cttattatgg ttattgcgat accaatactt gcggcactga tggatataat
    47461 gataaaaatc ataccttaag ggggatatac atgggaaaag gcatagtatt ttttatgata
    47521 atcattctgc tttccttatg tgccgctaac tgcgacgata cacaggatat tctgcaaagc
    47581 cagctggata atctggatat aagcgatata gaaaagtttg ttgatgatat caacagggaa
    47641 tctgaaggca tgctgcccga gatagatgta agggagatgg tattgggcct ttttaaggga
    47701 aatactcaaa acagcatcag agatatggga aaaggtattg caagcttgtt tttcaaagaa
    47761 gtaatcagca atttcagcct tatgggcaag cttctgatac tggctatttt ttgtgccata
    47821 cttcaaaacc tccataatgc tttcgaggag gataccatcg gccggttggc atacgggatg
    47881 tgctacatac tgataattat tctggctgta aagagtttta atgatgcaat gcttatcgga
    47941 gttgatacca ttaataaaat gacaggcttc atgcaggctc tgctgccaac attgcttgca
    48001 ttattggctt ctgtcggcgg gatttcttct tcggctgtgt tccagccttt gatgtttaca
    48061 tgcatcgcgc taataacaac atttataaaa gtatttgtca tgccggtaat acttttttcg
    48121 gcggttataa gcatattgaa caatctgtct gactccgtga atatttccaa gcttgcttca
    48181 ttgctgaaac aaattgcggt aggagctttg ggtttggtgc tcaccgcatt tacgggagta
    48241 ttgacggtac agggtattgc atcagcctca atagacggtg cgaccataag gactgcaaaa
    48301 tttgcggtgg acaactttat tcccatagtc gggaatttcc tctctgaagc ctttgatgcg
    48361 gttgtcagct gctcgatgat acttaagaac ggaataggga ttgcaggatt cattatcctg
    48421 cttcttatat gcagttttcc ccttataaag atgttgtccg ttgtattgat atataaggta
    48481 acaagtgcgc ttatccagcc gattcttgat aatcagatag tacaatgcct gaatgatatg
    48541 ggcaatgcaa tgctggttct tctgacatgt gttttagcag tagctgtcat gttcttcatg
    48601 gcacttacgg ttatcatggg agtcggaaat atggcggtaa tggtcaggta gtataggcag
    48661 gtgattttat ggctgacttt ttaagagaat ggatcataaa cataacggtg ataatcattt
    48721 ttataatgtt tctggatacc ataatcccca atagttccat gaaacgttat ataaacgtta
    48781 ttgtggggct tatgattata gtagttgtaa taaagccctt tgtgctgata aaggattatg
    48841 cagacagctt caatatgaag tttcttgaga cttcaagcta tatagaacaa ggcggttccg
    48901 cggcggacag tacggaaata agtaattttc aacgtcagat ggctgtagaa atttttgaaa
    48961 acaacctgaa aaagaaggtc ggaaggattg ttgacaacag cacggattcg gattacgagg
    49021 aagtatcgat agatcttcgg ctggagaagg attttgaaag caaggatttc ggtgaaatta
    49081 aatcgataga ggttattata agcaaaaggc cgaaagaggt gatagaagtt gacaggataa
    49141 aaattgcggc tgacgaagat gcggaagtta ataaaaatgt aattaataaa gacaaagttg
    49201 aatataattt gaataatagc agaataagaa gtgagattag ggatgggata agcaaagctt
    49261 tgggaatcag tgaatctatt ataggggtgg aggtaaggca gtaataatgc aagggaggaa
    49321 gtactatgaa attacatgag tggttgatgg ataaattaag gaaagtgaat tttcaaaagc
    49381 tgaacttttc aaaaaagacc actgcaaatc tgattatcgt attctgcttg gggttggctc
    49441 tgattttaat tgccgatttt tacagggata tccggaggga cagggcatca gaggaaggta
    49501 tttttagtga aattactcag gcggatacag gaacttcaga caacaacgct tctgactatg
    49561 tcagaaggct ggaggcggat ttgtcttcaa tactcagtaa gattcaggac gcgggcaagg
    49621 tttcggtaat gattacttta gagagcggag cagagataat tcctgcaaag gatgaatcga
    49681 cctccgacaa agtgaccaat gaaaaggata caagcggcgg aacacggata ataaacgaga
    49741 aaacaacgga tgacaaagtc gtattaactg ccgctcaggg aggtaattca aagcctttga
    49801 taataaagga gataaacccg gaagtaaaag gtgtaattgt agtagccgaa ggcgccaaag
    49861 attcaaaggt taaactgaaa atttcccaag cggtgcaaac ggtattggat attcctgcct
    49921 atagagtaac ggtatatgaa aggaattagt attaaatata aattcaggga ggtataatca
    49981 tgcaagtgaa gaaaaaaaat ttaataatat gttcaatgat aatactgctt tttgttgtcg
    50041 ggtatgctta caacagtttt acttccgaaa ggctgactga tgcaaatctt gacctggacg
    50101 cgcaagtaat cggaacagat gaaatgggga tgcctgctga aacggcggga acagaagagg
    50161 cggcggggcc aatagtcata gaggatagcg gcagtgaggc attagaaaca acagcagctt
    50221 cctttttctc ggaatacagg attgaaaggg ataagaacag aagcaaggaa gtagaaatgt
    50281 ggcaggatat aataaacagt gacaaggcgg aagagaattt caagaatatg gcgcagcagg
    50341 agattgtgaa aatcgtatcc cttaccgata aagaaatgat aattgagaat ctcataattt
    50401 ccagaggttt taacgatgca ttggtatttc ttaccgatga ctctgcaaca gtcatagttg
    50461 aagccaagga gcttacctcc tcaaatattg cacagataca ggacattgtg gtaagaaaaa
    50521 caaagctgga tcccaaaaac ataaaaataa tgaagaaaaa ctaaggcgta ttctatgaat
    50581 cttaggtaat gcttatcctg aagcccatgg aataaatact ataataatgt ataatataat
    50641 attggcagat acatatattt gtaaatggat taggattttg atataattgt tcatagaatc
    50701 tacaaggggg tgcaatattt ggaaaaggag ataaaattgg ataagtacgg taatgttaag
    50761 attgcagacg atgtagttgc aataattgct gaaatagctg ccagggaagt acagggtgtc
    50821 tattccatga gcggcgggat agctgacagc ataacagaga tacttggcaa gaaaaaccct
    50881 tcaaaagggg ttaaggttga agtaggcgaa aaagaagctg ccattgacct atacataata
    50941 gttgattatg gcgtgagaat acccgatgtt tcctggcaaa tacaggagaa cgtcaaaaag
    51001 gctgttgaga caatgaccgg cctgactgtg gtggaagtaa acattcatat acagggaata
    51061 aatatggata aggaaagcaa agaagaagag aatcctgaag gaaaatccaa ataggctttt
    51121 ataaactaac cctcttataa ttctattaca gtaaagaggg ttagtttaaa tatatgcagg
    51181 ttgtgaaata cataggagga tttatgagca gaaggcttgc cagagaattt gcaattcaat
    51241 tcctatatag tacggatttc aacaggaacg aaaatactga tgaaatgctt gaggattttt
    51301 atgagataaa aaaggaacag ggaaaagacg ggcagcagga ttccatgaag gaagcagaca
    51361 tgaagtttgc agaagaaata atcaaaggta ctattgggaa gcttcaggag atagatcaat
    51421 tcatacaaaa caatacaacc ggatggacaa aagagaggat tgccaaggtt gacctggcaa
    51481 tattaaggct tgctttgtat gaaattctgt ttcgcgacga cattcctgac agtgtatcta
    51541 taaatgaagc aattgagctt gccaagaaat acagcactga tgaatccggg agctttgtca
    51601 acggtgtgct gggcaaaatc ataagagaag cggatataaa ggtgtatgga acaaatgaat
    51661 aatgaaagaa tatttacggt taccgaggtt aataattata taaagcatct gatacagaat
    51721 gatgtactcc tgtcacattt gcatgtgaaa ggagagatat ccaatttcaa gcatcactca
    51781 tcggggcaca tatacttcac cttaaaggac gcagactcaa gaataagatg tgttatgttc
    51841 aaaggcaatg cttcaaagct caagttcatg cctgaggatg gctgtgccgt tattctgaga
    51901 ggttattttt caatatatga gagagacggg cagtaccaat tctatactga ggacatgatt
    51961 cccgaaggga caggctctct atataaggcc tacgaacagc ttaagaaaaa gcttgccgcc
    52021 atgggatatt ttgatgaggc aaggaagaag aaaatacctt ttatgccccg ggctgtcggt
    52081 gtggttacat cacctacagg agcagctgta agggatataa tttcgataat aagaagaaga
    52141 tgcccctgca tcgacgtata cttataccct gtgcaagtgc agggagcggg agcggaaaat
    52201 gaaattgcgg aagggatcag atattttaat tccaagggaa atgtggatgt catgataatc
    52261 ggccgcggcg gcggttccat tgaagagctt tgggctttca atgaggaagt ggtggcaaaa
    52321 gctgtatacg aatcggatat accggtaatt tcggcagtgg ggcatgaaac ggattataca
    52381 atttcagatt ttactgcgga tttaagagca gcgactccat ctgcggcagc ggagctggcg
    52441 gttcccgaca tgcagagcct gctgggaaag ttaagccagt atcaatatgt tctgaactct
    52501 gcaattgcaa attttataaa ggataataga agcagggttg agaggatttc cggagatatg
    52561 gttttcaggc agttggagag gaaagtcctc aatatgaggc agactcttga ttcactggac
    52621 aagtacctgg attacaacat gctgaatata atgaagggca gaaggaatga aatcgacaag
    52681 accatagaaa agctgcagct gttgaaccct gaggcagcta tgggcagggg ttacggcata
    52741 atcatgagaa aatccgacgg gaagctcctg acttctgtta aggatatgaa gcttaatgaa
    52801 gagctttata taaagctgaa agacggaaaa gcggaagtcc gggtcaaagg actggagagt
    52861 acataaaata tatataaaaa ccctacaata atatcttgaa atggagaaga ataatatgaa
    52921 tgagaatttt tcatttgagc aggctttaaa gaggctggag gaaatagtgg agactctgga
    52981 agcaggcaat atttctcttg aagagtcaat taaaatctat caggagggga ttgctctttc
    53041 aaagctttgc tccgggaagc tggaagaagc tgaggggaag gtaatggcta ttatgaaccg
    53101 cggaagcgag cagatggagg aattcagcat cagtcaaatc aaggaggcat agaattggac
    53161 cttaaggagt atctggcaaa taaaaggcat ttggtggaac agaagcttaa atccatgata
    53221 ccggcagaca atagtatacc ggttattacg gaagcgatga ggtatagcct gctggcaggg
    53281 ggtaagaggc tcaggcccat acttgccata atgtcctgtg agcttttcgg agggcaggag
    53341 gaagaagtgc ttccatttgc ctgctgcata gaaatgattc acacatattc acttattcat
    53401 gatgatttgc ctgctatgga taatgatgat ctcaggcggg gaaggccgac cagccacaag
    53461 gtttttggag agggctttgc tgtccttgcc ggtgatgcat tattgaacag tgcttttgaa
    53521 accatgacgg ccatattgat ggataacccc aagcctgaat atataagggc tatgaatata
    53581 atatgcaggg cctcgggaat atccggaatg ataggcgggc agtgcataga cctgtactat
    53641 gaaaacagga gtattgatat tgagactctg aaatccatgc atcggaaaaa gacgggtgcg
    53701 ctgattaaag cctcccttgc ggccggtgcg gttattgcaa gagcggatga aggtgacata
    53761 aaaaatatta tggaatttgg agatgcaatc gggcttgcgt accaggtaga agatgatata
    53821 cttgatgttt caggttctga agaaaagctt gggaaaagca taaacaagga tgcggaattt
    53881 cacaaatcaa attttatcag cttctacgga ctggataagt ccataagcat tgcggcagag
    53941 cttatcgggg aagccaagaa aaagcttgaa agatatgggg acaaaagctg ctatcttatt
    54001 gaactgtctg attatatcat tgacagggat aactaagctt tacaatatgt ataatagagt
    54061 acatatgaaa agtatctgag gagagggttg tttttgaatt tctttgtgaa agtagcagac
    54121 aacaaagcgc tgttggcctg tattacagca tggctgattg cgcagatact caaaataata
    54181 tttacctttt acagcagcaa gaagctggat ctgaccaggc ttgtaggttc gggaggcatg
    54241 ccgagttctc atactgcttt tgtaatggcg ctttctacgt caataggaag gatgtatggc
    54301 tgggactcgc ctatttttgc agtttcctta tgcttcgcct ttgttgtgat gtatgatgct
    54361 gcaggagtaa gaagggcagc gggaaaccag gcaagaatat tgaatatgat aatcgaggat
    54421 cttgcacaca ataagccgat taccgacgaa aagctgaagg agcttatagg acatactccg
    54481 aaggaagtta tggccggagc attattggga attgttgttg cgaatattat ggtgcgataa
    54541 aaataagtta gaaggttgag ttataaatgt ataaaattct ggatagtata aattctcctg
    54601 ccgatttgaa aaaactgagc taccaggagc ttgaagttct ttcaaaggag cttagggatt
    54661 atgtaataaa agtagtttcc gaaaacggag gccatttggc ttcaaatctt ggggtagttg
    54721 agctgactct ggcacttcat agggtttttg acacacccgg agacaaaata gtatgggatg
    54781 tggggcatca gtcttacatc cacaaaatta ttacaggcag aagagaaagc tttaaaactc
    54841 ttagaaaaat caatggaatc agcggtttcc ccaagaggga agagagtgtt tacgactcat
    54901 ttgaaacagg acacagcagt acatccatat ctgctgccct tggcatggca aaagcaaggg
    54961 atctggccgg agaaaactac tccgttgctg cagttatagg ggacggtgca cttacaggag
    55021 gcatggcctt tgaagcattg aaccatgccg gagactttcc caccaatctt attgtgatat
    55081 taaatgacaa taaaatgtca atatcccata atataggggg actgtccaac tatctgagca
    55141 agttgagaac agagccccta tacttcaaaa tgaaaggcag gatagaagct ttcctgcaca
    55201 ggataccggt aataggggga tctatgtaca gaggagcgga aaggctcaga gactggatga
    55261 aatacctgct ggttcccgga gttatttttg aggaactggg ttttaaatat ttcggcccta
    55321 taaacggcca tgatatcaga aagcttgaag atgtaataaa aagagcaaaa tcatataacg
    55381 ggtatcctgt attgatacat gtagtcacaa caaaaggaaa gggctattcc atagctgaaa
    55441 acaaaccgga tatatatcac ggcatcagcc ccttctttat tgaaaacgga gagcttaaat
    55501 caaaaaattc aagaaagact tattctgaaa tattcggaga taccatgtta aagctggcgg
    55561 acaaggacaa caaaatagtt gccgtgtcgg ctgcaatggc tgaaggaaca ggtttatccg
    55621 cctttgcatc aaaatataag gacagatttt ttgatgtagg tattgccgag cagcatgcgg
    55681 taaccttctg tgccggcttg gcctcagaag gtttcaggcc tgtttttgcg gtatattcca
    55741 cattcctgca aagggcttat gaccagatta ttcatgatgt ctgcatgcag aagctgaatg
    55801 tagtgtttgc cattgatagg gcaggtatcg tcggctccga tggggagact catcagggag
    55861 tatttgatat cacatacctg aggcatattc caaatatcag gataatgagc cccaaagatg
    55921 ggaatgagtt gagtgaaatg ctgaaaatgg ctttgtccct tgaaggcccg acggcaataa
    55981 ggtatccaag gggaaaggca aagaagtttg aatttgccca tgcacccctg gaattgggga
    56041 aaagcgaggt ccttacagag ggagaagacg gtgtcattat atcggaaggg aatatggttt
    56101 caatagccct ggaggtatgt gaaaaactga agaaagccgg caggaacttt accctggtta
    56161 acgccagatt cataaagccc ctggatgaag agctgcttgt cggactttcg aagaaacaca
    56221 agctcatata caccttggag gacaatgtag taagcggggg gttcggaagc agcattctgg
    56281 aattgttaag ccggctgagg gcggatgttg acgtcagaat aataggcttt gacgacagtt
    56341 tcatacctca cggtgaaaag gaagagcttt attccctgaa aaaaatagac agtgaaagtg
    56401 tattcaacgc aattatgaag gagtagagtg cattggcgga aaaggaaagg ctggacgttc
    56461 tcatacacaa atgcggtatg tccgaaagcc gtgaaaaggc aagcagtctg ataatgtcag
    56521 ggattgtata tgttaacggc atcaaggttg acaaaccggg aacaaagtta gccacggatt
    56581 caaatattga gatacggggt aatgccctgc cttatgtcag caggggaggc ctgaagctgg
    56641 aaaaggctct atgtgaattc ggcatcaacc ttaagggtaa ggtagctata gacattggag
    56701 catctacagg gggcttcacg gactgcatgc tgcagaaggg tgctttaaaa gtatatgcaa
    56761 tagatgtcgg ttacggacag ctggcttgga agctgaggac cgacagcaga gtggtatgta
    56821 tggagcggac aaacatcagg tatgttgctc ctgaagatat tagcgaatcg gcagacttct
    56881 catcaataga cgtttccttt atatccctga caaaggtgct tcccgcagtg atgcagcttt
    56941 taaatgagaa gggtgagatt gtatgcctta ttaaaccgca gtttgaggcg gggcgggaaa
    57001 aagtagggaa aaaaggtgta gtgagagaca aggacgtaca tattcaggta attgaaaaaa
    57061 taatttcttt ctgccgtgat gaaatgggac tatccatact ggggttaagc ttctcaccca
    57121 taaaagggcc ggaaggaaat atcgaatacc tgctgtacct gtcgaaaagg aaagatttat
    57181 ccgatacggc tcaaaggatt gatattggcg gtattgtgct gaaggcccat gaacttttgc
    57241 gttgattcgg gaattatata atgtaaatat ttgtagataa tttcaaatac ataatataaa
    57301 atatatttaa gacatttagg tggtgttgca gttaatgaag ctggggatta taccgaacat
    57361 aaataaagat aaggacttga acgtaaccag aagcattttg ctgtggtttg caaataaaga
    57421 tgcagtgctt atgctggaat ctgatattgc ggaaaagctg ggatacaaaa attccggctt
    57481 cagcagggag aagatatatt cctccagcga tgtggtaatt gttcttggcg gtgacggcac
    57541 attattgaat atagcaaggc aatcagcccg gtatgatgtc cccctgttcg gcataaatct
    57601 cggacacctt gggtttcttg cagaagccga aataagcgat atgtatttgt cactggataa
    57661 gattatggag ggtgattata atattgaaaa aaggatgatg ctggaagcat tggttgaaga
    57721 cgatgaaaat aacgggaaag aatttttagc tcttaatgat ttcagcgtta caaagggcat
    57781 gacttcaaga atgataacca tttctacata tataaacgac agtttttttg aactgtattc
    57841 ggcggacgga gtagtaatat caagccctac aggatccacc gcatactcgt tatccgcagg
    57901 aggccctata gtaagccccg aattgaaggt gctgataata actcctatat gtccccacac
    57961 aatacataac aggtcaatag ttgtgtcaga aaaagacgaa gtcaaaatag taataggcga
    58021 tgaaaaccct gaggtcatgc tttcggtgga cggacaggat tcatatagat taaattccgg
    58081 aaaagtagtt aggataagaa gttcaagctt cgttaccaac cttgtcaaac taaagcagag
    58141 aagctttttt gatgtactaa gaagaaaaat ttctgagcga tagttagaaa atggagtgga
    58201 ggtagagaaa tgaaaatatc aaggcatgca aagattttgg agattattga aaaacaccct
    58261 acggagacac aggaagagct ggcggaagaa cttaaaaaat gcggttataa cattacccag
    58321 gcaacggttt caagggacat caaggaattg aagctggtaa aggtacttga tgaggatggc
    58381 atctacaggt atgcatccct gaaggagcag gacagcatgc tgaatgaacg tcttgtgaag
    58441 gtatttgctg aatctgtttt aagtgttgat tttgcaggga atatagtagt tgtgaaaaca
    58501 ttctccggag ctgcaaatgc agccgcagaa gcaattgatg tattggattt caaagaagtg
    58561 gtagggactg tggccgggga tgatactatt tttattctaa taagaaattt ggagaatgtt
    58621 gaagtagtaa tagacaggct taagaagatg atgaaatagg tgatgcagta atgcttcttg
    58681 aaatgagtat caaaaattat gtcctgattg aacgacaact gattgccttt gaacccgggc
    58741 ttaatgtaat cacgggagaa acgggagcag gaaaatcgct tgttattgat gctctcgctg
    58801 cagttacagg cggaaagttc agtaaggaag acataaggac aggtgaggaa aaggcctctg
    58861 tagaagcgct gttttcactt gagggtgttt gggatatcaa gaatatcctg gaggaatacg
    58921 gtatagaaaa ggatgctgac aatagcctgg ttattacgcg ggaagtaaac gcacacggca
    58981 aatcggtctg caggttgaat ggtcagattg ttactctttc cgtacttaaa aggattgcac
    59041 agtatctggt ggatatagtg ggccagaatg aataccagct gcttttcaat tccgtcaagc
    59101 acggagagta tgttgacagc ttcggaagtg atgagatata tcaaatcaag agggatgtaa
    59161 agcatttatc ggacaagcat gccgaacttt cggaaaaact taaaagcgcc agcggcagtg
    59221 cttcggaaag agaaagaaag ctggacatgc tgaggttcca ggttgaagaa atcgaaagcg
    59281 caaggctgaa gataaatgaa gatgaagaac tgaggctgag acgccatctc ctggttaatg
    59341 ccgaaaagct gcttaaaggt gtgaatcatg tatataatgt tattttcagc ggagacagca
    59401 ataaaagggg agcattggat atgctttccg aaagcgcagg tactttgaga gaattggcag
    59461 cagcagatga aaagctggaa acctataaat cccggattga agagataatg taccagcttg
    59521 aggatataaa gggagaactg agggtatacc gggataatat tgagtttgat gacaatgaaa
    59581 tagatttttt ggaagaaagg cttgatcaga taaacaagct gaaaagaaaa tacggaagca
    59641 gcattgagga agtactcgtt tacagggata atgctgcgaa ggaacttcag gagcttattg
    59701 acaatgagaa gaatatggcg gaaatagaaa gtgaaataga aaaagtcaag gatcagtaca
    59761 tgataaaagc aaggctgctt tccggaacaa ggaaggcaaa ggcggaaata ctgcagaagc
    59821 ttatagagaa ggagcttcag gaccttaaca tgtacggatc aagatttgtt ataaaaattt
    59881 tgcaggatga aaatattatc agcccaaatg gcattgacaa gattgaattt atgatatctc
    59941 cgaatcccgg ggagcctgag aaggcactgt cgaaaatagc ttcgggagga gaaatgtcaa
    60001 gggtaatgct tgctatcaaa aatgcttttt cggaagtaga aaaggctcca tgcgttgtat
    60061 ttgatgaggt agacgcagga gtaggcggtc agacagccaa tatggtgggg caaaaaatta
    60121 aagcaatttc aaaaaacgca caaattcttt gcatcacaca cctgcctcaa attgcaagcc
    60181 ttacagataa tcatatattt gtagataaaa aagtacagga taataagact tacacggaaa
    60241 ttaaaatttt gaactttgaa gaaagagtgg aagaaattgc gcgaatgctc ggaggaaatg
    60301 aaaatattga agcatccctg gaacatgcta aacaattagt cgggaaaatg taaaaacatt
    60361 ttcccgattt gtatttattt tcattttcca agttaaaagc ttacgtgggt tcattatttt
    60421 tatgagaata ataggacttt aaaaaggtta acttaaatga gagaggtttt tgattgaagg
    60481 cgggctcttc tggggaaaca ggagagtgat gctattgcat aaaaagaatt acagaaatgt
    60541 ttttttgtac accactctga taataacaat actctgcctg acaaatgtat tattcactat
    60601 ttatagcttt cctgatgaga taaatgttat acaaggacag gaccgaaagc tgccgagcag
    60661 catggtttgc aatcttgagg tgattggaga ctataagcac agcatagatg ttaataataa
    60721 caaaataatt tcttcctttc ttttaatgga ggatgcagga aaaggaaagg caaaagccaa
    60781 tctaatgctt ttaggattac ttccggtaaa acaggttact ctgaatatga ttcctgatat
    60841 aaaggtagtg ccgtcagggg aagctatcgg cgtaaggata gaatcaaagg gagtgctggt
    60901 tgtaggttta tccagcataa ccgatactaa gggaagaaga tgcagccctg ctgcagatgc
    60961 aggctttgaa gtgggtgaca aaataataca gattgccgat aatcaggtgg aaaaggaaag
    61021 ggatattatt gattatttaa acaacaggca tgataaggag gaaagaacca aggttctggt
    61081 tgaaagagac ggaagcaggc aggatttata cgtcaagccg gtaaaatgcg aggaggatgg
    61141 catatacagg ataggtttgt gggtaaggga caatatagcc ggcgtcggaa caatgacctt
    61201 ttatgacccg aaaagcagga tgttcggtgc tctgggccac ggcattactg atgttgactc
    61261 aggggtattg gtggatatca atagcggaaa aataattaaa tcaaaaattg catcaattca
    61321 gaaggcgaag aaaacagtac ccggagagct tgtcggagta ttctacgaca gcgaggatga
    61381 atacgggatt atatataaga atacaagctt tgggatatat ggaaagcttt caaaaaaaca
    61441 aaagccggaa agaacaaaac cggtttctat aggtttgaac agccagatta aggaagggcc
    61501 cgcaaagata ttaacaacta tagaaggaaa taaggtagag gaatttgata ttgaaatcca
    61561 gaaggcaatg agacaaagaa actcagaatc caagagcatg ataattcgaa taacagatga
    61621 aaaacttata gaaaaaaccg gcggaattgt tcagggcatg tcagggagtc cgattattca
    61681 ggacaacaag cttgtaggat gcgtaaccca tgtgcttgtt aacgatccga caaaaggctt
    61741 cggtatttct atagaatgga tgctcaaaga ggcggatatt gaattggtag acagcctcaa
    61801 aagggcttcc ggagaataaa tttcagcaag ccggagtgcc gcagaactgc ggcactcctt
    61861 ttcgcgatac aagaagaatg cgccggggac acggggacgt ttctcccgag gacatgggga
    61921 cgtttcccct gtcccgcttt cagtgggaca cgcgaaacgt ccccatgtcc ccgcgtcccc
    61981 gtgtccccga cgcattattt ttctattgcg aaagtcgatt tttgtagaaa atggcataaa
    62041 catgctgcat ttatttgtat gaaattgttt aacgattatt aggcaaataa tagcaggaaa
    62101 tgaagctgct ttgtcgaata tgttttctat tgaataacaa tttatgggag gaataccaat
    62161 gaatttacaa aaaataaaaa tcctgatagc tgatgataat aaggatttct gcattattct
    62221 aagcgaatac cttactgcac agcctgattt tgaagtggtc ggagtagcca aggacggtct
    62281 tgaagttatt gaactgatag aaagtgaatt gcctgatgtt gtaatattag atataataat
    62341 gcctcattta gacggccttg gggtgctcga aaaactaaat tccatgcaga ttgaaaaagt
    62401 tccgaaggta ataatccttt ctgctgtcgg tcaggataaa attacacaaa aagcacttac
    62461 ccttggagca gaatactatg ttgtaaagcc gtttgatatg gatatcttct cgaagaggat
    62521 aagggaatgc tttggaattg ccaataatga aacagaaagg aaaaactata tacatcccgt
    62581 agctgtggcc tcgcctgcag ttactacacc cagggagctg gaatcggcta taacgaacat
    62641 aattcatgaa atcggcgttc ctgctcatat caaaggatac gtatacttaa gggaagccat
    62701 tacaatggtg gttaacaata tggaactctt aagcgctatt acaaaggaat tgtacccctc
    62761 tatctcgagg aagttcaata ctacacccag cagggtcgaa agggccataa ggcatgccat
    62821 cgaagtggcc tggggaagag gaaggataga tgttataaac aacctgttcg gctatacgat
    62881 acataatgtg aaaggaaagc cgacaaacag tgaatttatt gctatgatag ccgataaatt
    62941 gaggattgag ctgaagataa gctgaaggct tctaaaatta ccaagttaaa tactaaaatc
    63001 actgaatagt ggttttagta ttttttttgt gcaggaaagc atgctttaca aaataaacgc
    63061 gtcgtaagac gcatttttta ttttgttttg aaagatacta aaaacaatgg aggtgtggaa
    63121 atgaagctgc aaggcaaaat aaaagccggt gccagaacaa aaaaccttgt aaatttttta
    63181 tgccccggag acattgccgt catacgtcat gatgatattg atgaaatggc tgcccttgct
    63241 attgcaaata caggagtaaa agcagtcatc aatacaggga aaagcatgac gggaagattt
    63301 tcatcagccg gtgcagcggt actgctggag aataatattg ctgtcattga tgtggatttg
    63361 tctctggaaa tgttcaagga tgatgatatg ataactgtcc tggggaatga tattatcata
    63421 cggaaccgta tttacaaaaa tgtttgcacc gccgttaact ggaaatatat cggcaaaagg
    63481 aggcaggagt caaaagaaaa tgagaaatat gaaatactga agtttataga taatacgata
    63541 aaccttacag ccttggaagt tgacaagttt atttcagtta ataattaccc tgagctaagt
    63601 acaaggctta aaggcaggca tgcagtgatt gttgtaagaa acagcggtac tcttgatgaa
    63661 ttaaatgcat tgaaggctta tataaaaaaa tttaatcctg ttttcatagg ggtggacgga
    63721 ggagccgatc ttataataaa ttccggttat atacctgatt tgttgatcgg tgatatggac
    63781 agcgtatcag atattggaat atataaaagc agagaaatca tacttcatgc atatgaagac
    63841 gggcattgcc cctgccttaa aaggatcggc ggcatgaatg tgccctacaa gatatttgca
    63901 gtcagaggga ccagtgagga tgcagccttg atgctggctt acaacaaggg tgcggaactg
    63961 attgttcttg tcggagggca taattgcatg tatgatttca taagcaaggg aagagccgga
    64021 atggccagta catttatagt cagaaccttg ataggagaaa ggcttgttga ttgtaaaggg
    64081 ttcggaataa tatcagcatc cgaaaacgag gggaaggatg cgcaatgggc gaagatgtaa
    64141 gcgtaatcat tccggcatac aatgagtccg actgtattct tcggacgtta gaaggcctga
    64201 aaggaataga aaacataaaa gagataattg taatagatga cggttccaca gattctacat
    64261 atgaactctt gaagcccctg agggatatta tacttctgaa gaatgaatat aacgaaggaa
    64321 agggcagtgc ggtaagacat gccctggact atgcgaccaa taggtttgtt gctcttttag
    64381 acgcggatat ttgtgaaagc gctgcggaaa tatataagct tatcgaacat ataaatccct
    64441 gcggaaaatc aataattata ggccgcctgc ctgctcccgc aagaaaagga ggttttggaa
    64501 tagtaaagcg gttatcctcc ggctgcttca gggctatgac ttcccgaaat gtggattcga
    64561 tattgagcgg acaaagggtt cttcctctgg attttttaaa aagcattgaa ctgccttcgg
    64621 gctttggact tgagtttaag ctcaccctgg aagcagtaag aagaggatat gaagtgattg
    64681 aggttcctgt tgatatgcgc cacagggaaa cggggagaaa catttcgggc tttatgcaca
    64741 gggggaagca gtgcatcgat attattgaca ttattcgaaa tgagatgaag cgattgtgaa
    64801 acagggtaga tatatatgga tctgatagtt attgcggcag ttaccttaat aattgcacat
    64861 taccttatcc gcaatattat aaaaatgctg tgcagagcag aaagcaggat atgtatctct
    64921 tcaaattaca aaggtaaaag aataccttca atcggaggaa tagtatttgt tcccatccag
    64981 ttggctgcgg ttttccagct gctgctgcaa aggccggagc attcatacgg ctatctgagc
    65041 tatatgtttc ttatgctgag catgggtttt gcaggtgtca tagatgatct ggccggtgat
    65101 aaaagaataa aaggcttgat aaatcatgta aggagcactc ttaagagcaa tatgaccaca
    65161 ggttttatca aggcgctgat aggttttctt gcagcatgca caataagctt cagaacgacg
    65221 ggctcatttg ccgaacttgc tgcggatgtg cttgtaatca cactgtctgc aaatgccatt
    65281 aacctttttg acatgcggcc gggcagagca gtaaaggtct ttctggcaat atcccttatg
    65341 attttgtcct cgtcagcagg aaggccggcg gaagcgctgc cgttaataat acttgttatg
    65401 actgttctta tttacatgag ttacgattta agagaaatat gcatgctggg tgatacgggg
    65461 gcaaatatac tgggaatttc cctgggcttt tacagtacat tatttttgag cttcagcagt
    65521 aagcttatgc ttcttttact tcttatttta ctgaatattg ctgccgaaag gctgtctatt
    65581 accgcgttaa taagcaaaag caggatactc tcatatctgg acagcctggg aaggagacgg
    65641 acagataacc gatgatagat atattcaaag cacaagttaa ggaaatcata tatagggacg
    65701 tgtacatatc aaaattcagg atagaaagaa attccatgct gtgcaactgc attaactacg
    65761 aaatgctgac aggcagtgtc gaagtcaatg ataccgtttt gctgaataca acggcagtac
    65821 agttggggct tggctccggc ggttatcatt acgtactggc caatttaagc aaggacagct
    65881 tttcaaatat cgggctcggg catattatga aattgcgata tactcccatg caaataaatt
    65941 gtatggcggc ggaagcccag gaaagccctt atcataagat attcaatgat tttgaatcac
    66001 tcaggggcct gccggtgata acgggaacgc ttcacagcat gcttgccccc atagccctta
    66061 ccttgaaaca cattgcgggg aaagagagga ttgtctatat tatgacagac ggaggagcgc
    66121 tgcccatatg gatgagcgag acagtgaaaa ggcttcggcg cagcggggtg ctgtatgggg
    66181 caattacctt cgggaatgca ttcggtggag acttggaatg tataaatgta tatacggcac
    66241 tgattgcagc aaaggaaata atgaaggcag acgccgcagt aataaccatg ggccccggaa
    66301 ttgcagggac gggcaccagg tacggtttca gcggcattga gcaaggttat attattgatg
    66361 ccgtcaacaa tcttgacggc aaagccatag cagttcccag aatatctttt gccgacagca
    66421 gaagcagaca ttacggcttg agccatcaca ccatgatgac tttaggaaaa ctctgctgca
    66481 ctaaggcact tatagcactg ccgattcttg aaaaatcaaa ggatgattta ataagaaaac
    66541 agcttgagga aagcggagta ctgtccaggc accggttgat ccgcctggac ggtacagaag
    66601 tagagaagct gctggagacg gaaaaaagac agcttgagag gatggggaaa agcttttgcc
    66661 aggacaagga gtactttatt acttgcggtg tttccgcctt gcttgccatg gattctaatt
    66721 aagcctgtat ctgtaagaca aaatgtattc cctcaaggtg atttgcttat tttcaatcat
    66781 attcagctgc tccatcagct cattgaattt gcctacaaag tgaaaaccat ttttataaac
    66841 atatttaacc attacattta ccttccttgc atttgttaca tattatatgt gcaagtgtat
    66901 attttcatta ctgtttgaaa aaaatatgga atcataatat acttccttaa agcatattat
    66961 caagatagag aaacagtgcg gacaaaagct gagaggaggt ttttcttttg ccggacagcc
    67021 ttaatgattc aatcagaaga catcttgcag ataatatatg gatttactta atagtcatgt
    67081 ttttattcat tcttggaata agccttggag cattggctgt aaataatatc gatgaagcgg
    67141 caaaaacaga tgcaataacc tatctggaag gtttccttga tctgacgaac caaaatcagc
    67201 ttcagtccga agccatattg aagcagtcaa taaaatacaa cctttattat gcattgatac
    67261 tgtttttttc aggggtagtg tacattggca tacttgttat tccgatactt gtcgctttca
    67321 ggggtttttg catcgggttt tcaatagctt ttctc
//
LOCUS       DAOQUR010000008        67077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_481_length_67077_cov_11.915789, whole genome shotgun sequence.
ACCESSION   DAOQUR010000008 DAOQUR010000000
VERSION     DAOQUR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 67077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67077
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_481_length_67077_cov_11.915789"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1288
                     /locus_tag="PKI50_02875"
     CDS             83..1288
                     /locus_tag="PKI50_02875"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNT02692.1"
                     /translation="MSDLNKLKNISNVRTFKENEYIFHEGDTGNDMYILLSGRVSIFI
                     KSKDNLQIKLCDICAGGFFGEMSLLEGEPRTATALTIENTVALSVNKDNFEAFVVEQP
                     SMAFKLLKGLSSRIRALDEELSRLKKGSQYVIPPADSTDALSDSAPCISASNPEDLKG
                     VLYHDEHGTYEGESPDTDTFYIYDKKITCPICGKEFELSVTSQSKLRLNSIDFDFRHR
                     YDGFEPLWYNVLICPFCYFAGYSNEFDNHMDNLCKAFTEKTAPIRKKLAFKYSQPRKL
                     DEVFTAYYLALHSAKALSAGPLKSARIWLQLSWLYKDAGDEKMYAIATDQAFEHYYDV
                     LYGSSLTKLTDEQDQQCCLILGELYLLKNDRNQAVRQFHAAIRRGGGNALYNKQAQDR
                     IYYVKHEMK"
     gene            complement(1398..2132)
                     /gene="cwlD"
                     /locus_tag="PKI50_02880"
     CDS             complement(1398..2132)
                     /gene="cwlD"
                     /locus_tag="PKI50_02880"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HNT02693.1"
                     /translation="MRIYIIDRSKAVGYMIKFAWILVLFISICFIGKSTTAAFSLPIR
                     STVVVIDAGHGGRDPGASGSSGITEEEVNLKIALKLRRLIEQGGGTAIMIREDNSGLY
                     TEGGNRQGTRKSEDLKNRHALINSCGADILISIHLNSFPQSQYFGAQTFYMKNEEKSR
                     KLAEGIQNELIKVLDRGNERKAKATDSMYILKNNNMPGALVECGFLSNREEERLLDDE
                     HYQEKIAWSIFVGIVKYMEEEKKAIE"
     gene            complement(2245..2856)
                     /locus_tag="PKI50_02885"
     CDS             complement(2245..2856)
                     /locus_tag="PKI50_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT02694.1"
                     /translation="MINVLAIYGSHRRGQNSDMLADKILEGIGSNEASIERIYASSQG
                     IKSCTACERCYELGYCVIKDEMQQVYEAMDKADIVVSSSPVYFYSVSSHMKKLIDRCQ
                     AVWASKYRTEGSLISKKHRIGYIVCTAGEPEGKSRFECAVMVFELLYKCINVEFVGSL
                     LVSDVDNQHVRGREDIRQKAFEAGRLLRESISSIKSHNLHNSI"
     gene            complement(2955..3284)
                     /locus_tag="PKI50_02890"
     CDS             complement(2955..3284)
                     /locus_tag="PKI50_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HNT02695.1"
                     /translation="MNRLGDTEYKSISLPRLNNLKPNIESTALKLMEEAGELAQLIGK
                     YRGLNGEKCRMEERQCVDRIAEELLDVAQVAISMMFVLEESYSIDIQEKVESHIKKLK
                     GKGYIKG"
     gene            complement(3310..3969)
                     /locus_tag="PKI50_02895"
     CDS             complement(3310..3969)
                     /locus_tag="PKI50_02895"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT02696.1"
                     /translation="MEFRNGQFLVILSPFTDSEAYGYINSITEDSLILSVNPAGFLRN
                     GWGILCIFLENDDIYEFYSEITYLEGNEIIIKKPIENKLNTIEKRRFARVDCEIGFVA
                     RPVTINNVSVGKLGKTFLGTIKNISAGGVLAETNLCLPVDSVFSFKLKTNYFIDCTAK
                     VLRVNELPSEKKFQMGCKFVNMRIEDFKAISMFTFKEQLKKKRKQFYETAAKQKIHIS
                     C"
     gene            complement(4224..4484)
                     /gene="rpsI"
                     /locus_tag="PKI50_02900"
                     /pseudo
     CDS             complement(4224..4484)
                     /gene="rpsI"
                     /locus_tag="PKI50_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(5002..5130)
                     /locus_tag="PKI50_02905"
     CDS             complement(5002..5130)
                     /locus_tag="PKI50_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02697.1"
                     /translation="MLRYEGDETFLSDVIGKSALQPGDRVTLSFSAQDALVLPAQA"
     gene            complement(5114..5566)
                     /locus_tag="PKI50_02910"
                     /pseudo
     CDS             complement(5114..5566)
                     /locus_tag="PKI50_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(5555..5962)
                     /locus_tag="PKI50_02915"
                     /pseudo
     CDS             complement(5555..5962)
                     /locus_tag="PKI50_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(5959..6279)
                     /locus_tag="PKI50_02920"
     CDS             complement(5959..6279)
                     /locus_tag="PKI50_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02698.1"
                     /translation="MEPAPAEEGGKLGGELNLLVMPGYEEEKIIKPFEEKYGVKINAK
                     VYSTSDQMFSLLANSAEGEWDIVTPDTPWVAKLVDADLIDELNPKIIRKLIISMTAGR
                     ILTR"
     gene            complement(6716..7360)
                     /locus_tag="PKI50_02925"
     CDS             complement(6716..7360)
                     /locus_tag="PKI50_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT02699.1"
                     /translation="MQNCRLFSGLSDRQIKLAKQQLDGVINSYREAEVIVEEDAIVNR
                     IGILLQGNAYISKFTMDGKELLMQKLVPFYLVCAEVAFTRKQNAPYTIYCEKDTDIYW
                     FSIEKITKNGLIDDGIREILLRNIMLYIADENMRKYHKIEAISIKGIRERIVHYLALQ
                     QKINGSNQFYIKFDREKLANFLGMNRSVLSHELKRMEKEGLITFKKNYFEIFRL"
     gene            complement(7561..8598)
                     /locus_tag="PKI50_02930"
     CDS             complement(7561..8598)
                     /locus_tag="PKI50_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT02700.1"
                     /translation="MKRLLALTMAIIMLMVAFSGCAPANSQPDVITDFTVTDMAGRQV
                     TIPGEIKSIATFGSVGVLNAFVELMGSGDLLCNQMPARFTKSDKWKMQYEFAPQIKDA
                     PLLANSNDELLLEEVLKLQPDLCITMTKETAEQLGENGIPCIYLEWKQTEDVKAAVNL
                     MGEILKKEDIAEDYLAYFDSMVQEASDLTAKLTDEDKKTVLYGNVTNLTQPHVIAEWW
                     IDVAGGISVTNDGKTTGQSREYTQEELLLWDPDIIIVTERDMIDKIKEDKLYTDVTAV
                     KAGSIYYIPTVAHTWGNRTVEQPLTVFWTLNKLYPELVSDNMLAEKVSYFYSHFFKYD
                     LTPQQISEIIE"
     gene            complement(8646..9437)
                     /locus_tag="PKI50_02935"
     CDS             complement(8646..9437)
                     /locus_tag="PKI50_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02701.1"
                     /translation="MIRVENVIFSYRHSAPVLRDVSFNFERGESLCVLGPNGTGKTTL
                     FRCLLNLHSIQGGKIFIDGKDAASLRPRVRARLISYVPQASALTFPYTAWETVLMGRV
                     AHQKLGASVSLKDRIAAEAAMERLNVLCMAQCRFHELSGGERQLVLIARALAQETDCL
                     ILDEPTASLDYANQIRILQVIRQLAEDGYAILMTSHLPDQAFLAATRVLMLKDGIVFA
                     DGTPEDIITSRNLSSLYGLDVYVADAQAVFNSGTQESKVCIPSMS"
     gene            complement(9439..10455)
                     /locus_tag="PKI50_02940"
     CDS             complement(9439..10455)
                     /locus_tag="PKI50_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT02702.1"
                     /translation="MKKQIRYPLMMVMFLCGLVAAFFISFLIGRYPIAIRTVADIILS
                     RFFTITAYWEGTLEKVVLHVRLPRILLAILVGGSLSMAGASYQTLFKNPMVSPDILGV
                     SAGAGFGAALAMLQNGSWAGIQFFALTFGLLAVGLAYLIASLSGGRQNLTVLILAGIV
                     TSSLFQSLLSVVKTLADTDNQLPSITFWLMGSLGKGNNDDVLIMLPVVTASAFVLFLF
                     RSHIDALSAGEDEALSMGVNVPLVKIVVIVSSTLLTVVSVSICGIIGWVGMVVPHIAR
                     ALTGASYGRLCSLSFLIGALFLLLIDNIIRGIPGVELPLGVMTALIGTPAFAVMLARI
                     KRGW"
     gene            complement(10452..11288)
                     /locus_tag="PKI50_02945"
     CDS             complement(10452..11288)
                     /locus_tag="PKI50_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNT02703.1"
                     /translation="MSKNEILLESVHIVKGRLKSELSNITIDRAVLGLFFSGVKLSTG
                     HGGLCFTPVKDIPAAVCCPSSAAAMPLSGKLRGRSAEQYLEDVFSDNILKKTLGIAAL
                     NALSNLIWELEPPEGYSIAIGMDAFDNVDVSQYKKSVVIGALVPMLKKLITGGCDFRV
                     LEQDPRTLKSGELPYYAPAEDAHKYVPDADLLVITGVTILNDTLPGILNMAKPGAQII
                     VTGPTASMLPDALFSRGVTMVGGILVTKADELLDIISEGGSGYHFFGKSAERTVIFKN
                     QP"
     gene            complement(11369..12568)
                     /locus_tag="PKI50_02950"
     CDS             complement(11369..12568)
                     /locus_tag="PKI50_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNT02704.1"
                     /translation="MYLNQKQFLMRSEALKELFGRWNPEIATESIAVSEAVGRVLSDA
                     VSSVNTLPVCRSSKLDGIAVSFEALMQASGGQFPWTGTIAHVSADTGDDFDDAFDTVI
                     AVERIVRHSDGSLTITSKEPIKQGQNIRPRGDTLKEGQQLLKKDTLLQPVHLAFLSTA
                     GVSTVKVYKKPVVSFIPTGNELIPIGVSPKRGQNIESNSLMVRTMLAQWGAKAHCCPI
                     VKDDNVLMEETLLNACSKADIVLINGGSSMGSEDYASRLLQKHAEWFQHGIRCIPGIP
                     IALAIIDKKPVINLPGPTLASYYAMDWCVKAMVFHMLKQDMPLRRTVRAVLKQKVQKP
                     SHLEMYVRLRVKCINGRYTAEVLSPQKGLAQLLADCNALFIAPIGKETFEAGEMVRAE
                     LLSGFDI"
     gene            complement(12836..13231)
                     /gene="rpsI"
                     /locus_tag="PKI50_02955"
     CDS             complement(12836..13231)
                     /gene="rpsI"
                     /locus_tag="PKI50_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNT02705.1"
                     /translation="MAKKVQYFGTGRRKQSVARVRLVPGEGKIIINDREIDDYFGLET
                     LKLIVRQPLILTDTLSKFNVLVNVIGGGFTGQAGAIRHGISRALLEADDTLRPMLKKA
                     GFLTRDPRMKERKKYGLKKARRAPQFSKR"
     gene            complement(13253..13687)
                     /gene="rplM"
                     /locus_tag="PKI50_02960"
     CDS             complement(13253..13687)
                     /gene="rplM"
                     /locus_tag="PKI50_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNT02706.1"
                     /translation="MKSYIPKTDEIQRKWYVVDATDKVLGRLASEVAKILTGKNKPIY
                     TPHIDTGDYVIVINCDKVVLTGKKLDQKMHRTVTTRVGSMKETPYRKFMAEQSDKAVY
                     EAVKGMLPKNSLGRKMLKKLRVYKGAEHENAAQMPEALELDI"
     gene            complement(13912..14661)
                     /gene="truA"
                     /locus_tag="PKI50_02965"
     CDS             complement(13912..14661)
                     /gene="truA"
                     /locus_tag="PKI50_02965"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNT02707.1"
                     /translation="MRNIKLIIEYDGTNYCGWQVQENGPSIQAEIEKALLSVTGEAIS
                     INGSGRTDAGVHARGQAASFITESSIPPEKFAYALNNKLPEDIVIKESGEVPLDFHAR
                     FSAVGKKYSYLVINSRFPSALLRNHAYHVNYCERLDIDKVKTAAEAFIGTYDFSGFMA
                     AGSKVSNTVRTIYDISIEQEGELIRFIYKGSGFLYNMIRIITGTLLYAGIGKINPEDM
                     KDVILSKNREQAGITVPARGLYLEEVYYNLS"
     gene            complement(14709..15500)
                     /locus_tag="PKI50_02970"
     CDS             complement(14709..15500)
                     /locus_tag="PKI50_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNT02708.1"
                     /translation="MIKDITIGQYIPGDTAVHNLDPRIKIIITFVFITLLFLVGNYFS
                     YLYIIAFMGLAIGLSKISLKFIAKGLKPLIVIIVLTVILNAFMTQGEVIYRIWGLSIT
                     KEGLYQAGFMGLRLIFLITGASLLTLTTSPIALTDGIEKLLNPLKRIGVPAHELAMMM
                     TIALRFIPTLLEETDKIMKAQMARGADFETGNLINRAKAMVPLLVPLFISAFRRADEL
                     ALAMEARCYRGGEKRTRMKQLKLEFRDYSAMMIIAVFCFLVIWSR"
     gene            complement(15507..16355)
                     /locus_tag="PKI50_02975"
     CDS             complement(15507..16355)
                     /locus_tag="PKI50_02975"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNT02709.1"
                     /translation="MPVKVENLTHIYMKDSVFEHVAIDNISFEIQDGEFIGLIGHTGS
                     GKSTLIQHLNGLLKPSSGKVFIDGEELGSKEVQMKKIRQKVGLVFQYPEYQLFEETVY
                     KDVAFGPRNLGLSEDEVDARVRKAIELVGLDFDSVSNVSPFELSGGQKRRVAIAGVLA
                     MEPKVLILDEPAAGLDPKGRDDILKGFKTLHETQKITVVLVSHSMEDIANLVDKVLVM
                     NKGKIAYFDTPRNVFGEAETLEKIGLGVPQMTYLVRELRKKGYPIADCINIDEAKNEL
                     LKLFKR"
     gene            complement(16340..17179)
                     /locus_tag="PKI50_02980"
     CDS             complement(16340..17179)
                     /locus_tag="PKI50_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNT02710.1"
                     /translation="MANMITTENVRFEYGTNSGSAKPALDDININIAKGEFVAILGHN
                     GSGKSTLAKHFNALLLPGAGTVYVKEMNTKNEKLLWDIRQTAGMVFQNPDNQIVATIV
                     EEDVAFGPENLGIPPLEIRRRVDDSIKTVDMTEYAKHSPHLLSGGQKQRVAIAGIIAM
                     KPECIILDEPTALLDPVGRKEVLNTIIKLNKEEGITIILITHYMDEAIQADRIYVMKN
                     GKIIMEGKPKDVFKEVETVKAAGLDVPQVTELSHRLNREGLQLSPDILTVEEMVVELC
                     RLK"
     gene            complement(17372..17713)
                     /gene="rplQ"
                     /locus_tag="PKI50_02985"
     CDS             complement(17372..17713)
                     /gene="rplQ"
                     /locus_tag="PKI50_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNT02711.1"
                     /translation="MAGYRKLGRPSDHRKAMLRNLVTSFLEHGKIVTTETRAKETQSI
                     AEKMITLGKRGDLHARRQALGYITKEDVVKKLFDDIAPKYKERNGGYTRIAKVAPRRG
                     DAAEVVVLELV"
     gene            complement(17781..18728)
                     /locus_tag="PKI50_02990"
     CDS             complement(17781..18728)
                     /locus_tag="PKI50_02990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNT02712.1"
                     /translation="MIEIEKPKIECIEMSEDYTFGKFVVEPLERGYGTTLGNSLRRVL
                     ISSLPGVAVTSIKIDGVLHEFSTIPGVAEDVTEIILNIKNLALKMHGENTKIIKIDVQ
                     GEKEIKASDIICDADVEIINKDLHIATVDEGHRLYMEMTVEMGRGYQISEKNKQPGQP
                     IGIIPIDSIFTPVRKVNYLVENTRVGQVTDFDKLVLEVWTDGTINPDEAISFGAKILS
                     EHLKLFITLTEHVGNVEIMVEKEEDKKEKVLEMTIEELDLSVRSFNCLKRAGINTVEE
                     LTQRTEEDMMKVRNLGKKSLEEVQQKLEALGLSLKTSED"
     gene            complement(18838..19464)
                     /gene="rpsD"
                     /locus_tag="PKI50_02995"
     CDS             complement(18838..19464)
                     /gene="rpsD"
                     /locus_tag="PKI50_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNT02713.1"
                     /translation="MARYTDAKCRLCRREGSKLYLKGDRCYTDKCAVARRAYAPGQHG
                     QGRKKVSEYGIQLREKQKVRRIYGILEKQFRDYFVEAERQKGVTGENLLRLLELRLDN
                     VAYRMGFGESRAEARQLVRHGHFTVNGRKVDIPSYQTGLNDLIAVKPGSKSTDKFKGL
                     AENAAGKTAPQWLSINTEMMEARIVALPAREDIDLPIEEHLIVELYSR"
     gene            complement(19485..19883)
                     /gene="rpsK"
                     /locus_tag="PKI50_03000"
     CDS             complement(19485..19883)
                     /gene="rpsK"
                     /locus_tag="PKI50_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNT02714.1"
                     /translation="MVTKPKAKKTRRRKERKNVERGAAHIQSTFNNTIVTLTDSTGNA
                     LSWSSAGSLGFRGSKKSTPFAAQMAAEVAAKGAMEHGLKIIEVFVKGPGAGREAAIRS
                     LQAAGLEVSLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(19910..20278)
                     /gene="rpsM"
                     /locus_tag="PKI50_03005"
     CDS             complement(19910..20278)
                     /gene="rpsM"
                     /locus_tag="PKI50_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNT02715.1"
                     /translation="MARIAGVDLPRDKRAEIGLTYIFGIGRATSNRILAEAGVSPDTR
                     IKDLTEAEIGRLREVIDRSVKVEGDLKRDIALNIKRLMEIGCYRGLRHRRGMPVRGQR
                     TKTNARTRKGPKKTVGVRRK"
     gene            complement(20372..20485)
                     /gene="rpmJ"
                     /locus_tag="PKI50_03010"
     CDS             complement(20372..20485)
                     /gene="rpmJ"
                     /locus_tag="PKI50_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNT02716.1"
                     /translation="MKVRPSVKPICEKCKIIRRKGKVMVICENPRHKQKQG"
     gene            complement(20503..20721)
                     /gene="infA"
                     /locus_tag="PKI50_03015"
     CDS             complement(20503..20721)
                     /gene="infA"
                     /locus_tag="PKI50_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNT02717.1"
                     /translation="MSKQDVIEVEGKVVEALPNAMFQVELENGHKILAHISGKLRMNF
                     IKILPGDKVTIELSPYDLTRGRITWRGK"
     gene            complement(20750..21013)
                     /locus_tag="PKI50_03020"
     CDS             complement(20750..21013)
                     /locus_tag="PKI50_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT02718.1"
                     /translation="MQDISLGQIVISAAGRDKGCKLVVLCIIDEKYVYISDGDIRKVD
                     KPKLKKVKHLKKLNHVAENIKEKLESCEKLSNSEIRKLLKSID"
     gene            complement(21033..21779)
                     /gene="map"
                     /locus_tag="PKI50_03025"
     CDS             complement(21033..21779)
                     /gene="map"
                     /locus_tag="PKI50_03025"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNT02719.1"
                     /translation="MISIKSPREIELMRIAGRIVAETHELLSKAIRPGITTLELDTIA
                     EEFIRKRGAIPAFKDYNGFPASICSSINEQVVHGIPGPTALKDGDIIGIDIGAVYDGY
                     YGDAARTYWIGSIDKETERLLKVTEESFFKGIEYALPGNRLSDISHSIQKHVESNGFS
                     VVRDFVGHGIGRNMHEDPQIPNYGLPHKGPRLAAGMALAIEPMVNQGKYAVKVREDGW
                     TVVTADGKPSAHYENTIVITNGKPEILTLL"
     gene            complement(21776..22426)
                     /locus_tag="PKI50_03030"
     CDS             complement(21776..22426)
                     /locus_tag="PKI50_03030"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNT02720.1"
                     /translation="MRIVLLGSPGSGKGTQASFIEKEFGIPHISTGDIFRDNISRNTA
                     IGAEAKKFIERGLLVPDSLTVSIVESRLLEEDCNEGFLLDGFPRNIAQAEALDKDLEK
                     MGKKLDAVVNLEVSAETIIERMAGRRICSDCGESYNLCFLKPAADGICDKCGGKLYIR
                     EDDKPETVMNRLNVYKNQTFPLIEYYKGKGILISVNGEQPADKVYGDICRVLERFK"
     gene            complement(22490..23746)
                     /gene="secY"
                     /locus_tag="PKI50_03035"
     CDS             complement(22490..23746)
                     /gene="secY"
                     /locus_tag="PKI50_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNT02721.1"
                     /translation="MFNTLRNAWKIPDLRQKMLFTLVMLIIFRLGSFIPVPGMNTEAL
                     SRLIQSGGLLGFFNTISGGAFDNFAIFAMSITPYINSTIIMQLLTIAIPSLEALAKEG
                     EEGRRKIAQYTRYGTVVLALIQAAALSYGLRGYLLRTDAASVILVALSLTAGTAFLMW
                     LGEQINEKGIGNGISLLIFAGIISRVPGGALKIFELYKVKSTNLIEIAFFLIFAILII
                     MAVIFIQEGERRIPVQYAKRVVGRRMYGGQSTHIPLKVNQAGVIPVIFAMSLIAFPGQ
                     IAQFFPNSWAYRFFNTYLGWGSWAHSILYALLIIGFTYFYTAVSFNPIEVANNIKKYG
                     GFIPGIRPGKPTSDFITKSLNRITLVGAFFLAVIAIVPIFLMNFTGIKVYFGSTSLLI
                     VVGVAMDFVKQVEAQMVMRNYQGFLK"
     gene            complement(23748..24188)
                     /gene="rplO"
                     /locus_tag="PKI50_03040"
     CDS             complement(23748..24188)
                     /gene="rplO"
                     /locus_tag="PKI50_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNT02722.1"
                     /translation="MKLHELKPAEGTTKSKKRLGRGTATGQGKTAGKGQKGQKSRSGG
                     GVRVGFEGGQMPLYRRLPKIGFTNVFRKEYAVVNLSDLERFDNGTIVNPEVLKEAGLV
                     KAMLAGVKILGNGDLTKNLTVQAHKFSKTAAEKIAAAGGKVEVI"
     gene            complement(24205..24384)
                     /gene="rpmD"
                     /locus_tag="PKI50_03045"
     CDS             complement(24205..24384)
                     /gene="rpmD"
                     /locus_tag="PKI50_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNT02723.1"
                     /translation="MAKLKVKLIKSTIGRKDDQIATVKALGLKKINSEVEHNDTPQIR
                     GMIQKVIHLVEVKEM"
     gene            complement(24397..24897)
                     /gene="rpsE"
                     /locus_tag="PKI50_03050"
     CDS             complement(24397..24897)
                     /gene="rpsE"
                     /locus_tag="PKI50_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNT02724.1"
                     /translation="MQPIDSSKLDLKEKVVAINRVAKVVKGGRNFRFSAIVVVGDMNG
                     YVGVGTGKSVEIPEAIRKGIEDAKKNLIKVSIVGTTIPHEVIGVAGAGRVLLMPAKEG
                     TGVIAGGPARAVLELAGIKDIRTKSLRSNNPRNMVNATIQGLASLKTVEDVAKLRGKA
                     VEEIIG"
     gene            complement(24916..25284)
                     /gene="rplR"
                     /locus_tag="PKI50_03055"
     CDS             complement(24916..25284)
                     /gene="rplR"
                     /locus_tag="PKI50_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNT02725.1"
                     /translation="MITKASKNSTRQKRHLRMRKKVFGTSERPRLNVYRSLNNIYAQI
                     IDDTKGVTLVSASTLDKELKDNPGHGGNKESAKAVGTLVAKKALDAGIKQVVFDRGGY
                     IYHGRVKELADAAREAGLEF"
     gene            complement(25306..25848)
                     /gene="rplF"
                     /locus_tag="PKI50_03060"
     CDS             complement(25306..25848)
                     /gene="rplF"
                     /locus_tag="PKI50_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNT02726.1"
                     /translation="MSRIGKLPIEIPQGVEVKIADDNTVSVKGPKGQLIKKMHEDINI
                     EKKDGKLLVTRPTDQKMHRALHGLTRALLNNMVEGVSKGFEKVLEINGVGYRAAKSGK
                     KLTLTMGYSHPVEMDDPEGITTEVPAPNKIIVRGADKEKVGTHAANIRAVREPEPYLG
                     KGIKYSDEVIRRKEGKAGKK"
     gene            complement(25876..26274)
                     /gene="rpsH"
                     /locus_tag="PKI50_03065"
     CDS             complement(25876..26274)
                     /gene="rpsH"
                     /locus_tag="PKI50_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNT02727.1"
                     /translation="MVTTDPIADMLTRIRNANTVKHETVDIPASNMKKAIAQILLDEG
                     YIKGYEIIGDGSTNSIRISLKYGSNKQKVITGLKRISKPGLKVYAKNEDIPKVLGGLG
                     IAIISTSKGIMTDKNARLQGVGGEVLCYVW"
     gene            complement(26455..26640)
                     /locus_tag="PKI50_03070"
     CDS             complement(26455..26640)
                     /locus_tag="PKI50_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNT02728.1"
                     /translation="MAKKSMVLKQQRTPKYKTRAYTRCKICGRPHAYLRKFGICRICF
                     RELAYKGQIPGVKKASW"
     gene            complement(26670..27209)
                     /gene="rplE"
                     /locus_tag="PKI50_03075"
     CDS             complement(26670..27209)
                     /gene="rplE"
                     /locus_tag="PKI50_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNT02729.1"
                     /translation="MARLKEKYLNEVAPAMMQKFQYKSVMQIPKLEKVVINMGLGDTK
                     DNAKGLDSAVSELGTITGQKPVITKAKKSVAAFKVRQGMSVGAKVTLRGNQMYEFLDK
                     LFNIALPRVRDFRGVSVNSFDGRGNYSLGIKEQLIFPEIEYDKVDKIRGMDIIFVTTA
                     ETDEEARELLRLLGMPYAS"
     gene            complement(27233..27571)
                     /gene="rplX"
                     /locus_tag="PKI50_03080"
     CDS             complement(27233..27571)
                     /gene="rplX"
                     /locus_tag="PKI50_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNT02730.1"
                     /translation="MANAEVKKLHVKKGDTVVVISGKDKGKKAKVLIALPQEDKVLVE
                     GVNMSVKHKKPGKNMQQGGIIHQESPIFSSKVMLWCDKCKKGVRLGHRILDDKSKVRY
                     CKSCGEILDK"
     gene            complement(27586..27954)
                     /gene="rplN"
                     /locus_tag="PKI50_03085"
     CDS             complement(27586..27954)
                     /gene="rplN"
                     /locus_tag="PKI50_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNT02731.1"
                     /translation="MIQQETRLNVADNSGAKELLCIRVVGGSTRRYGNIGDVIVATVK
                     DATPGGVVKKGEVVKAVIVRSVKGKRRPDGSYIRFDDNAAVIIKEDKQPRGTRIFGPV
                     ARELREKDFMKIISLAPEVL"
     gene            complement(27977..28231)
                     /gene="rpsQ"
                     /locus_tag="PKI50_03090"
     CDS             complement(27977..28231)
                     /gene="rpsQ"
                     /locus_tag="PKI50_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNT02732.1"
                     /translation="MDRNNRKTRIGKVVSDKMDKTIVVGIETLVRHPLYGKSIKRTTK
                     FKAHDENNECKIGDKVKIMETRPLSKDKRWRLVEIIEKAK"
     gene            complement(28251..28457)
                     /gene="rpmC"
                     /locus_tag="PKI50_03095"
     CDS             complement(28251..28457)
                     /gene="rpmC"
                     /locus_tag="PKI50_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNT02733.1"
                     /translation="MKANKFRDMSVKELEQKLNELKGELFNLRFQMATGQLENPMRVK
                     DVKKSIARIKTILKENELKSVQAK"
     gene            complement(28447..28890)
                     /gene="rplP"
                     /locus_tag="PKI50_03100"
     CDS             complement(28447..28890)
                     /gene="rplP"
                     /locus_tag="PKI50_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNT02734.1"
                     /translation="MLIPKRVKHRKQMRGSMSGKATRGNFIAYGEFGLQATEPGWITN
                     NQIEAARQAMTRYTKRGGKIWIKIFPDKPVTQKPAETRMGSGKGAPEYWVSVVKPGRV
                     LFEVAGVEEEIAREALRLAQNKLPIKCKFVTRRETEEMVGDTNES"
     gene            complement(28890..29588)
                     /gene="rpsC"
                     /locus_tag="PKI50_03105"
     CDS             complement(28890..29588)
                     /gene="rpsC"
                     /locus_tag="PKI50_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNT02735.1"
                     /translation="MGQKVNPHGLRVGIIKDWDTKWYADDKSFADFLVEDNKIREFLK
                     TKLFTSGISRVEIERTAGKIKINIHVAKPGMVIGKGGAGIEELKKQLEKLTNNKGVTV
                     NITEIKVPELDAQLVAENIAAQLEKRISFRRAMKQSMQRTMRVGAKGIKTMCAGRLGG
                     AEIARSEWYREGTIPLQTLRANIDYGFAEAKTTYGRIGVKVWIYKGEVLPQAKTRKSE
                     QAKLNEAAAEGGEA"
     gene            complement(29605..29940)
                     /gene="rplV"
                     /locus_tag="PKI50_03110"
     CDS             complement(29605..29940)
                     /gene="rplV"
                     /locus_tag="PKI50_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNT02736.1"
                     /translation="MEARAIAKYIRISSRKVNIVIDLIRGKKVSEAFAILKHTPKAAS
                     VLLEKLLKSAVANAENNYEMNVDKLYISEIYATQGPTLKRFRAASHGRGVRILKRTSH
                     ITLAVKEKE"
     gene            complement(29985..30269)
                     /gene="rpsS"
                     /locus_tag="PKI50_03115"
     CDS             complement(29985..30269)
                     /gene="rpsS"
                     /locus_tag="PKI50_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNT02737.1"
                     /translation="MSRSTKKGPYVDEKLLKKIIDMNKTNKKVVLKTWSRSSTIFPEM
                     VGHTIAVYDGRKHVPVYMTEDMVGHKLGEFAPTRTFRGHGHHTERSSALK"
     gene            complement(30291..31124)
                     /gene="rplB"
                     /locus_tag="PKI50_03120"
     CDS             complement(30291..31124)
                     /gene="rplB"
                     /locus_tag="PKI50_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNT02738.1"
                     /translation="MAIKKYNPTSPARRNMTVSTFEEITKTEPEKSLVEILKSKAGRN
                     NYGKITVRHQGGGHKRQYRIIDFKRDKVDIPAKVAAIEYDPNRSANIALLYYADGEKR
                     YIIAPNGLKVGDTIISSENADIKVGNALPLKSIPIGTVIHNIELKAGKGAQLVRAAGN
                     SAQLMAKEGEYAQVRLPSGEVRMIRIECKATIGQVGNLDHENISIGSAGRKRHMGIRP
                     TVRGSVMNPADHPHGGGEGRSPIGRPGPVTPWGKPTLGYKTRNNKKASSKFIVKRRNE
                     K"
     gene            complement(31139..31426)
                     /gene="rplW"
                     /locus_tag="PKI50_03125"
     CDS             complement(31139..31426)
                     /gene="rplW"
                     /locus_tag="PKI50_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNT02739.1"
                     /translation="MNAYDIVRKPVITEKSMSEMADKKYTFIVDVNANKTEIKNAVEE
                     IFKVKVDKVNTVKVLGKMKRMGKYEGRRPGYKKAIIKLTKDSKGIEFFEGL"
     gene            complement(31426..32049)
                     /gene="rplD"
                     /locus_tag="PKI50_03130"
     CDS             complement(31426..32049)
                     /gene="rplD"
                     /locus_tag="PKI50_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNT02740.1"
                     /translation="MPNVALYNISGKQVGEIELNENIFGVNVNVEAMQQVVKMYLANQ
                     RQGTQSALTRAEVRGGGIKPWRQKGTGRARHGSIRSPQWKKGGIVFAPKPRSYRYTVP
                     KKIKRIAMKSALSSKANENSIVVIEELNFDAPKTKQVVSLLENLKIDSKTLIVLADNN
                     ENVVKSARNIDGVKATFVNTLNVYDILKYDKFVITKDAVKKVEEVYA"
     gene            complement(32082..32711)
                     /gene="rplC"
                     /locus_tag="PKI50_03135"
     CDS             complement(32082..32711)
                     /gene="rplC"
                     /locus_tag="PKI50_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNT02741.1"
                     /translation="MKKAILGLKVGMTQIFDNEGKAVPVTVIAANPNIVVQKKTVENE
                     GYEAIQVGFDDKAEKKVTKPLKGHFAKAKVATKRYLREFRLEDAASYEVGQEIKVDIF
                     KEGDRVDISGVSKGKGFAGAIKRHNFHRGPMKHGSKYHRWAGSMGASSSPSRTIPGKK
                     LPGHMGAENTTVVNLEVVKVDPEKNIILVKGGIPGIRGSLVMIKDTVKA"
     gene            complement(32863..33171)
                     /gene="rpsJ"
                     /locus_tag="PKI50_03140"
     CDS             complement(32863..33171)
                     /gene="rpsJ"
                     /locus_tag="PKI50_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNT02742.1"
                     /translation="MAKQKIRIRLKAYDHILIDQSAERIVETAKRTGAKVSGPVPLPT
                     EKEIVTILRAVHKYKDSREQFEMRTHKRLIDILNPTPKTVDSLMRLDLPAGVDIEIKL
                     "
     gene            complement(33636..34838)
                     /gene="tuf"
                     /locus_tag="PKI50_03145"
     CDS             complement(33636..34838)
                     /gene="tuf"
                     /locus_tag="PKI50_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNT02743.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITVVLSKYGQATA
                     TKYDEIDKAPEEKERGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDAELIELVEMEVRELLTE
                     YEFDGDNTPIIAGSALKALECGCGKEDCEWCKSVHELMDAVDTYIPTPQRQTDKPFLM
                     PVEDVFSITGRGTVATGRVERGTVKVSDTVEIVGLADAPRSTVVTGVEMFRKLLEQAQ
                     AGDNIGVLLRGVQRNEIERGQVLAKPGSIKPHTHYKAQVYVLTKEEGGRHTPFFNGYR
                     PQFYFRTTDVTGVIELPAGVEMCMPGDHIEMTIKLITPIAIEEGLRFAIREGGRTVGS
                     GAVASVIE"
     gene            complement(34926..36995)
                     /gene="fusA"
                     /locus_tag="PKI50_03150"
     CDS             complement(34926..36995)
                     /gene="fusA"
                     /locus_tag="PKI50_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT02744.1"
                     /translation="MPRQFSLEKTRNIGIMAHIDAGKTTTTERVLYYTGKTYKLGEVH
                     EGTATMDWMEQEQERGITITSAATTAEWKDHKINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFCAKGGVEPQSETVWRQADKYNVPRIAYINKMDIMGADFYRVVEMMKERLGANAV
                     PIQLPIGSEDRFVGIVDLIGMKAHIYKDDLGKNMEVGEIPSDMMDKAKEYREKLIEAV
                     SEMNEDLMMKYLEGEEPTEQEIKDAIRAGTISVKINPVVCGSSYRNKGVQLMLDAVVD
                     FMPSPIDIPPIEGTTVDGEEKIVRHSDDKEPFSALAFKIMVDSYVGKLAFFRVYSGMV
                     EAGSYVYNSTKGKRERIGRIVQLHANHREEIKEVYTGDIAAIVGLKDTTTGDTLCDEE
                     NAVILESMDFPEPVINVAIEPKTKAGQDKMGIALQKLAEEDPTFRTYTDAETGQTIIA
                     GMGELHLDIIVDRMMREYKVEANVGKPQVAYKETIRKKVQVEGKFVRQSGGKGQYGHV
                     WLEIEPKEPGTGFEFVNKIVGGVIPKEYIPAVQRGVEEATMNGVLGGYPVLDVKVTLY
                     DGSYHEVDSSEMAFKIAGSMAFKDGMKKADPVLLEPIMKVEVTVPEEYMGDVLGDINS
                     RRGKIEGMEARSGAQVIRGFVPLSEMFGYATDLRSRTQGRAVYSMETDHFEEVPKSIA
                     EKILGEK"
     gene            complement(37104..37574)
                     /gene="rpsG"
                     /locus_tag="PKI50_03155"
     CDS             complement(37104..37574)
                     /gene="rpsG"
                     /locus_tag="PKI50_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNT02745.1"
                     /translation="MPRRGHISKRDVLPDPLYNDKIVTKLINNVMYDGKKSVGQRIVY
                     GAFEILREKTGKDPLEVFETAMNNIMPVLEVKARRIGGATYQVPIEVRPDRRQALGMR
                     WLIEYARKRGEKTMKERLAGELLDAANNTGSSVKKREDTHKMAEANKAFAHYRW"
     gene            complement(37706..38080)
                     /gene="rpsL"
                     /locus_tag="PKI50_03160"
     CDS             complement(37706..38080)
                     /gene="rpsL"
                     /locus_tag="PKI50_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNT02746.1"
                     /translation="MPTISQLVRKGREKVEYKSTAPALKESPQKRGVCTVVKTTTPKK
                     PNSALRKIARVRLSNNIEVSGYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DAAGVADRKQSRSKYGAKRPKK"
     gene            complement(38201..38443)
                     /locus_tag="PKI50_03165"
     CDS             complement(38201..38443)
                     /locus_tag="PKI50_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNT02747.1"
                     /translation="MQELKAIHRRTVGLKQTVKAIKSGTVKKVYLAEDADDFIKQSVL
                     DVCRDRDIQIIYVSNMKELGDACGIDIGASTAAIKD"
     gene            complement(38643..42167)
                     /gene="rpoC"
                     /locus_tag="PKI50_03170"
     CDS             complement(38643..42167)
                     /gene="rpoC"
                     /locus_tag="PKI50_03170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNT02748.1"
                     /translation="MFELNNFEAIKIGLASPEKIREWSRGEVKKPETINYRTLKPEKE
                     GLFCEKIFGPTKDWECHCGKYKRIRYKGIVCDRCGVEITKAKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLILDMSPRALEKVLYFASYIIIDSGDTPLSKKQLLSEKEYRENIE
                     KFGNRFRAGMGAESIRELLLDLDLEKLSKELRQDLKGSTGQKRLRTVRRLEVVEAFKN
                     SGNKPEWMILEAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKLFQCGLPNEMALELFKPFVMKKLVNDGLAHNIKSAKRMVER
                     VKPEVWDILEEVISEHPVLLNRAPTLHRLGIQAFEPVLVQGRALKIHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSANNILAPKDGRPVVSPTQDMVMGVYYLTIYVPG
                     HKGEGKEFTDLNEMLMAYQLDEVSLHAKVKVRITKEINGRMVSRFVENTVGRFIFNQS
                     IPQNLGFVERNTEEDMFLLELDPRDKDGNLMPVDKSLLGKIVDKCYRMHGNTNTAIVL
                     DEIKRLGFHYSTKGAITISISDMDIPMAKKELLSKADERVDKIEKMYRRGLISNDERY
                     EQVIETWKIATEDVTKALMDNLERLNPVYMMANSGARGSVNQIKQLCGMRGLMADTSG
                     RIIEIPIKANFREGLDVLEFFTSTHGARKGLADTALRTADSGYLTRRLVDVSQDVIAR
                     EEDCGTEDGLMVSEIKDGNEVIEKLKDRLMGRYASSDILHPETGEVLVKRNELITEKK
                     ANSIVKLGIKKVPIRSVLTCHSKYGVCTKCYGVNLATGDPVHIGEAVGIIAAQSIGEP
                     GTQLTMRTFHTGGVAGDDITQGLPRVEELFEARKPKGLAIISEISGVVKIIDQRKKKD
                     VVVLAEDGDSRTYTIPYGSRLRVADGDVIEAGDEITEGSVNPHDILKIKGVAGVQSYL
                     LQEVQRVYRLQGVDIADKHIEVIVRQMLRKVKIEESGDTDLLPGGLFDIFKYEEENQR
                     VSAEGGEMATGKRVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKIDPLVGLKE
                     NVIIGKLIPAGTGMKKYMDISIKQVEAE"
     gene            complement(42194..46243)
                     /gene="rpoB"
                     /locus_tag="PKI50_03175"
     CDS             complement(42194..46243)
                     /gene="rpoB"
                     /locus_tag="PKI50_03175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT02749.1"
                     /translation="MLVHPISAGRNQRISYSKIDEVMDMPNLIEVQKNSYNWFLEEGL
                     REVFNDVSPIQDYTGNLILEFVDYSLDGKPKYSVEECKERDVTYSAPLKVKIRLINKE
                     TNEVKEQEVFMGDFPLMTDTGTFIINGAERVIVSQLVRSPGVYYAETIDKSGKKLYSS
                     TVIPNRGAWLEYETDSNDILWVRIDRTRKIPITVFLRALGFGTDEQIDGILGEDDRIK
                     ATLQKDGTKNEEEGLIEVYKRLRPGEPPTVESAKSLLEALFFDAKRYDLAKFGRYKFN
                     KKLSLSSRLTGQKLDRELADPRTGEILAEEGEKITRDKAVKIQNAGINEVYVTAEGKT
                     VKILGNNTVDINKYLSFDVSDLNIKELVNLSVLKDILQKAGSESEIKEEIRNRIKELI
                     PKHILIDDIVASINYNINLSNGIGNKDDIDHLGNRRLRSVGELLQNQFRIGLSRMERV
                     VKERMTIQDIDVVTPQQLVNIRPVAAAIKEFFGSSQLSQFMDQTNPLAELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLIGSLSTYAKVDEYGFIETP
                     YRTVKTDDNGEKYVTDEIVYLTADVEDEYVIAQANEPLDEEGRFVNRRVTARDANEIV
                     IVPTMEIEYMDVSPKQVVSVATAMIPFLENDDANRALMGSNMQRQAVPIIKPDSPIVG
                     TGMEYKSARDSGVIGLANSDCVVEKVSANEIVMRNSLGEKERYKLLKFLRSNQGTCIN
                     QRPVARKGEVLKKGDIIADGPSTDMGEIALGRNVLIGFMTWEGYNYEDAILISEKLVK
                     EDTFTSIHIEEYEAEARDTKLGPEEITRDIPNVGEEALKDLDERGIIRIGAEVRAGDI
                     MVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGESGIIVDVKVFTRENGD
                     ELPPGVNELVRCYIAQKRKISVGDKMAGRHGNKGVISRVLPEEDMPFMPNGTPLEIVL
                     NPLGVPSRMNIGQVLEVHLALAAKALGWKIATPVFDGATEESILETIECANKVLQVKK
                     NLRIIKERILIADKQNDEDYEGQQLAETDIRSEIKENVEIIYELLNHEIYDIDSKFTS
                     LREMIKSSDLTELKSVEKILKAFGETPIDGIELNHTGKATLRDGRTGEYFDNDITVGY
                     MYILKLLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGSAHTL
                     QEILTVKSDDVVGRVKTYESVVKGENIPEPGVPESFKVLIKELQSLALDVKVLTEEHE
                     EITIRESVEEDVEDLGADMEVREDMDFSSLSYKGETVTPEDLYEDYEMESEGAEDFDA
                     EIDLDDLLIGDNEVEEEVEEEVEEDGEEF"
     gene            complement(46537..47430)
                     /locus_tag="PKI50_03180"
     CDS             complement(46537..47430)
                     /locus_tag="PKI50_03180"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNT02750.1"
                     /translation="MIIGDVLNGKYRIERLLGKGGTGSVYLCTNIEIGNKWAVKHIPP
                     DKINDKTLSEIEILKRLYHPSLPRVVDAFRNEQGIYIVESNIEGSSLDKLLKRHGSFD
                     LETVTDWFLELCDILKYLHGMKPRPIIYRDMKPSNIILAQGVRLVLVDFGISQEYCEY
                     ESGDTFFAGTSAYAAPEQLINGGKTEQRTDIYNMGVTIYHLLYGRLPGSADYSLRRRK
                     SRAASNVGRIISKCMENKPEDRYQRVEDIQKELESVKNMLAVNKARQKAVFKLEIASI
                     ALLSAASYTAAILGALYSLSK"
     gene            complement(47457..47672)
                     /locus_tag="PKI50_03185"
     CDS             complement(47457..47672)
                     /locus_tag="PKI50_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02751.1"
                     /translation="MKLHMIYISIGVFTLILNLALTLHIIRGHRNKSCRKNPDCEEVI
                     TFRLTGAEGIEDATTAAAPCDDTLPLE"
     gene            complement(47699..49036)
                     /locus_tag="PKI50_03190"
     CDS             complement(47699..49036)
                     /locus_tag="PKI50_03190"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HNT02752.1"
                     /translation="MRNTVLDFEYSYDNNAAGSYLVLKIDSESALLKHQVEILCQNPN
                     SAFMPFHVRRENENTFIYYNITSKISLSHYIERKSLNRAGLLDLLRSIAKNLMLSGNY
                     LLDLSGFIIHPDYIFINPATAEASLLYVPVSCNRNVTEILRSFFKDIVVNSSNADDNA
                     RDNYLQRILNYLKSDTFSLIDFNRLIVDLRSSGELYEYGQGAAVQEAAAGISTPSSRS
                     PKERAYEDAGKSSNIRSIMLLQLIFLVAAAAACLFLISRKMGDIVSIAGVVIISAALD
                     ILVINRISGSRGKRAATDDTQRIAQDKVKRSAGRHDEVLRSRYKPVFSSPDVVKACDT
                     VCISEIPKDCRPYLESIGPNRVERVIINKDGFIIGRLGSMADHIIQGGTIGKLHAEIT
                     FNEGFYYIRDLNSKNGTFVNGVKIPSNREYGIKDNDRIKFSNYEYVFKMQEEL"
     gene            complement(49058..49486)
                     /locus_tag="PKI50_03195"
     CDS             complement(49058..49486)
                     /locus_tag="PKI50_03195"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNT02753.1"
                     /translation="MSHYLPYLTIIILLAAAAIIDIRKKRIPDKLVLAGTIAGMAFVL
                     IDPQREFLNSILGGVTAGLVLLLIHYITKGGLGLGDVKLFGCSGIFLGFERIISAMLI
                     SAILSGLFSIVLICLNPDNKKSEIPFAPFILAGVLAVIIP"
     gene            complement(49512..50453)
                     /locus_tag="PKI50_03200"
     CDS             complement(49512..50453)
                     /locus_tag="PKI50_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02754.1"
                     /translation="MYKGYKKLRSRGSITVEACAVFPVFISVFFLLLFLIKFTCTGMV
                     LDHVVNEAAKEIAAAAYPISFINELEDEKHREYGNAKIPTLEEELEKLAGEISGGSSD
                     NLFRAIVSGELEGTDLTEAVQDILKDYGKGAIGNVVGRITPAYWDMKSDAKYYIAEAF
                     IKEHLKSPLINRENIRLRLVEFPQGEEEYNARSQRAIYSELGLTPDKDFSRDDVVLQL
                     EYEYLVRLPFIEPFNIRMIHTAVERAWINGSFGILTAEDEGLDLEPEGSIVYITRTGI
                     RFHEGDCRYLRKSRIPILIEEAEGSGYTPCKVCKPLG"
     gene            complement(50465..52519)
                     /locus_tag="PKI50_03205"
     CDS             complement(50465..52519)
                     /locus_tag="PKI50_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02755.1"
                     /translation="MYQRDGSIRKEKGAITVFLALIFMSLIIFAGTVIDIVRIIAADR
                     RVQSTLNTSARSVLADYDSELMGSYGIYGVNAAEGNVKEDFYRYLNVNLKERHKNINF
                     INISIDYEDIEIQGMENLLKHETFNRQVQEYMKYRTVISATGSLIEQLQNIKLNKGIE
                     FAKSEKITRDKANELRAKVSEVNIKLTSIKKKLTNISAEKLEDIRDELSGALEINSLI
                     CNGNAGGLLADYIQSRDETNIKAEEGQFVENQSREFDSIKKNNENLTPGIQMYIEEID
                     KTLLLVKPLQNELERMEGKLESLKDELDELKEKLSDVQEDDAGVAEEIDETREEIDEV
                     KDKIRRLESEIDEEMSDLKMKLGRISLEGYSLKEEAVQLPDKKTEELKSFISKTKKEI
                     KEALFRRLEKGWLIEPEEFEDSSLITGENFGEMDESTYFSASMREEEAEKSNDAIIKT
                     LEKLAKAIGDVASNAFEKVNTIEYVMDKYTFLTSGTERGHYFRKSEVEYIISGSDIEE
                     GYSPIKNTEYYVVTKVLLQVWALRFALDTIDAFIRSTVVFPPQRLAFALAEGALDSSL
                     DIFNMLNGEAVPICPKSFTTVRLKYSDHLKILLLMKPEEEILRRARQLIQVNIKQVVD
                     AKTELPRSDFRLGDYSTVISASVKAKVNLFFLPLLKVDRLLPGSFENGKYLIYKQIYV
                     GY"
     gene            complement(52549..53250)
                     /locus_tag="PKI50_03210"
     CDS             complement(52549..53250)
                     /locus_tag="PKI50_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02756.1"
                     /translation="MLDKHICDRGSYTVEASMVFVTIMLALLALLFTFLYMGQRASLE
                     SFASFAAQQGAELWLDSRRNMEDGKINNKRPEDSMGYRIFDNLLFSSTTYEGYFEREA
                     SDNGKTRVVFKMDAGNNLPGHKALLIGEDLCKRIESTILKPESTRIRITFNNNALRGR
                     LIVELIQEIKVPLGGIKEFFDGKDTLTLSVQSEAAVSEPDEYIRNVDLILELSKRLGE
                     ELDLGELAGRIGAKR"
     gene            complement(53299..53511)
                     /locus_tag="PKI50_03215"
     CDS             complement(53299..53511)
                     /locus_tag="PKI50_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HNT02757.1"
                     /translation="MLKEVKGFIVEEDGISTVEIVVIIAILIGIALLFRKSITAYVQR
                     VIRNFIDTAPAVENNEIDMTAPLPTD"
     gene            complement(53556..54419)
                     /locus_tag="PKI50_03220"
     CDS             complement(53556..54419)
                     /locus_tag="PKI50_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT02758.1"
                     /translation="MLPVFITEIIVLGFICFYVKGKYEDFTETGFKKGHIYKVLAPAA
                     LFITDKLIAGRFIAYERKLGIKLAELYGGRDIKHMLKLHFVHKVIHMIIALLSVTLLG
                     ALMEKPDIIYAIFALSALGIVFYVSDRELDEKIKKRNFNIRYDFPDFLNKMVLLINAG
                     MTVPRAWEKIVRDRKNITPLYEELRTTYIEIMNGKPEMEAYEDFAKRCRVKEITKFIT
                     AVIQNLKKGNSDLVPLLKLQSNECWQLRKNMAKKLGEEASTKLVLPLMIMFVGILIIV
                     TLPAVMQLRYL"
     gene            complement(54423..55238)
                     /locus_tag="PKI50_03225"
     CDS             complement(54423..55238)
                     /locus_tag="PKI50_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT02759.1"
                     /translation="MDLRKWPAVSTEYEYGRGTAAHASLADYDTYIMSLKEKLLCIFM
                     AGTVIFATGYIFYRSALLSLLLTPFALLYPGFRTKEIIKKRKNELNLQFREALYSLSS
                     SLSAGKSIETAIKGSIKELSIQYPNNDTYILIELEHISRRIEMNETIEEALADFAARS
                     HLEDIINFADVFLICKRTGGNLVQVVKNTAEIITEKIDIRQEINLLLMEKRLEHKVLN
                     LMPVLIVLLLSASAEEFMAPVFSEPLGRAAMTFSLVLFGAAYFVSRKIMDIEV"
     gene            complement(55250..56488)
                     /locus_tag="PKI50_03230"
     CDS             complement(55250..56488)
                     /locus_tag="PKI50_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNT02760.1"
                     /translation="MDDDAKNLVINNLRKEIRESLDIKSQVTDEDINELITDKVFEEA
                     RLRSLSIREKQELISVLFNSIRKLDILQPLIDDKSITEIMVNGEDNIFIEKHGRTYSI
                     ESSFESRQKLEDVIQSVVSKVNRVVNEASPIADARLADGSRVNVVLPPIALNGPILTI
                     RKFSERPISMKDLIEWGSITEEAAEVLERLVKAKYNIFICGGTGSGKTSFLNALSDFI
                     PRDERIVTIEDPAELQIKNIKNIVKLETRNANIEGKGEITIRDLIRASLRMRPERIII
                     GEVRGAEAFDMLQAMNTGHDGSISTGHGNSTADMLSRLETMVLSGAMMPLEAIRQQIA
                     SAVDIMIHLSRFRDSTRKVVEIVEVTDCREGMIGLNPLFEFVERGGSMSEKVEGCLVR
                     TGNTMKNLAKLRMAGLNEVI"
     gene            complement(56567..57742)
                     /locus_tag="PKI50_03235"
     CDS             complement(56567..57742)
                     /locus_tag="PKI50_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02761.1"
                     /translation="MARLNLVIADCDESYARGLFEYINSNHSSSFMVSCFTKLDSFAG
                     YFEKQPQTDILAISSDFYDISVEYPNIKLKVILSGEALNREYPGFQAIRKYSTGDKLI
                     RDLLYLYSRANPQEIRLSSCSKDTEFIGVYSPAGGTGKTTIATSLSTQCAELGLKSFY
                     LNLESIQSTGMFFGLGSKRNLSYVYYYIKEKSRNLSFKMEGIKSTDDGVEYFNPPDSP
                     MEYEEINPEELEQLIKGIKGMGCCDYVFIDMSSNFDSKNYKIMKLCDRIVLVTLREPI
                     ASYKERVLLNELAKLRDTDGGSISDKFITVINKYKNKGDEDFESFGENAAAAVKIPEY
                     SRTLIREDGRIFIENDDFRKAVNRIIDIITRRKDVTTSTSDGNPFRCPTGSEHISPR"
     gene            complement(57745..58272)
                     /locus_tag="PKI50_03240"
     CDS             complement(57745..58272)
                     /locus_tag="PKI50_03240"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNT02762.1"
                     /translation="MIAIVFKLIIVSMLMLLALVSDFRTYSVKNYITYGFMLIGLAVN
                     LAIEGIGGLVFSLQGIIIPAACLFVLYITRIVGAGDIKLLCAVGAVMGAAFALYAAIY
                     SFIFGGFIASGLMLTRENASERFNHLFVYLKSCFLTMDILQYTDLKNRQDGGKFHFSI
                     AIASGTAAAIIMRGV"
     gene            complement(58517..58900)
                     /gene="rplL"
                     /locus_tag="PKI50_03245"
     CDS             complement(58517..58900)
                     /gene="rplL"
                     /locus_tag="PKI50_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNT02763.1"
                     /translation="MASEKTLKFIEEIKNMTVLELAELVKAIEEEFGVTAAAPVAVAG
                     PAAAGAAAAPAEEQTEFNVVLAEVGAEKIKVIKVVREVTGLGLKEAKDLVDGAPKPVK
                     EAVSKQEAEEIAAKFAETGAKIEIK"
     gene            complement(58926..59429)
                     /gene="rplJ"
                     /locus_tag="PKI50_03250"
     CDS             complement(58926..59429)
                     /gene="rplJ"
                     /locus_tag="PKI50_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNT02764.1"
                     /translation="MAKNLEQKKQQVSEVKEKLQKSNAVVLVDYKGISVEDVTDLRKR
                     FREAGVDYKVYKNTLFKRAASELGMEALHEYLQGTVAVAFGYDDAVAPAKTINQFVKD
                     NPKTPISIKAGYIEGKIMNVDEVKALGDLPSREVLIAMLLGGLQGSIRNLAYALNTIK
                     EKKEAEA"
     misc_feature    complement(59480..59599)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(59607..60302)
                     /gene="rplA"
                     /locus_tag="PKI50_03255"
     CDS             complement(59607..60302)
                     /gene="rplA"
                     /locus_tag="PKI50_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNT02765.1"
                     /translation="MFRGKKYQESAKLIDRTKLYDPKEAMELVQQTAKAKFDESVEIH
                     VKLGVDPRHADQQVRGVVVLPHGTGKTLKVLVFAKGDKAKEAEAAGADYVGAEELVAK
                     IQGENWFDYDVVVATPDMMGVVGRLGKILGPKGLMPNPKSGTVTFDVERAINEIKAGK
                     VEYRVDKTSIVHVGFGKVSFGTEKLLDNFRTLMEAVIKAKPAAAKGTYLKSVVVTSTM
                     GPAIKINPTRVTD"
     gene            complement(60351..60776)
                     /gene="rplK"
                     /locus_tag="PKI50_03260"
     CDS             complement(60351..60776)
                     /gene="rplK"
                     /locus_tag="PKI50_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNT02766.1"
                     /translation="MAKKVSALVKLQVPAGKATPAPPIGPALGQHGVNIMGFCKEFNE
                     RTAKQAGLIIPVVITVYQDRSFTFILKTPPASVLIKKAAGIESGSGIPNKTKVGKISK
                     AKVREIAEMKMPDLNAGSIETAMSMVAGTARSMGISVED"
     gene            complement(60819..61337)
                     /gene="nusG"
                     /locus_tag="PKI50_03265"
     CDS             complement(60819..61337)
                     /gene="nusG"
                     /locus_tag="PKI50_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNT02767.1"
                     /translation="MEERGKWYVVHTYSGYENKVMANILKIVENRNMQDVIFEVKVPV
                     EEQVEIKGGKKKITQKKVFPGYVLVKMIMTDESWYVVRNTRGVTGFVGPGSKPVPLTD
                     AEIKNLGVEKIPIKLDAKVKDHIRVISGPFENFVGVIQEINNEKHKVKVMISMFGRET
                     PVELEFTQIERL"
     gene            complement(61407..61607)
                     /gene="secE"
                     /locus_tag="PKI50_03270"
     CDS             complement(61407..61607)
                     /gene="secE"
                     /locus_tag="PKI50_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNT02768.1"
                     /translation="MAGAAKRFDVAKSFKETKAEVKKVTWPNRKELLQHTEVVITSII
                     LVGAVLWVIDFIFGKVLNLLIK"
     gene            complement(61629..61778)
                     /gene="rpmG"
                     /locus_tag="PKI50_03275"
     CDS             complement(61629..61778)
                     /gene="rpmG"
                     /locus_tag="PKI50_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNT02769.1"
                     /translation="MRVKITLACSECKQRNYDSMKNKKNNPDRMEMNKYCRFCRKHTS
                     HKETK"
     gene            complement(62136..63338)
                     /gene="tuf"
                     /locus_tag="PKI50_03280"
     CDS             complement(62136..63338)
                     /gene="tuf"
                     /locus_tag="PKI50_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNT02770.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITVVLSKYGQATA
                     TKYDEIDKAPEEKERGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDAELIELVEMEVRELLTE
                     YEFDGDNTPIIAGSALKALECGCGKEDCEWCKSVHELMDAVDTYIPTPQRQTDKPFLM
                     PVEDVFSITGRGTVATGRVERGTVKVSDTVEIVGLADAPRSTVVTGVEMFRKLLEQAQ
                     AGDNIGVLLRGVQRNEIERGQVLAKPGSIKPHTHYKAQVYVLTKEEGGRHTPFFNGYR
                     PQFYFRTTDVTGVIELPAGVEMCMPGDHIEMTIKLITPIAIEEGLRFAIREGGRTVGS
                     GAVASVIE"
     gene            complement(63492..63568)
                     /locus_tag="PKI50_03285"
     tRNA            complement(63492..63568)
                     /locus_tag="PKI50_03285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63533..63535),aa:Thr,seq:ggt)
     gene            complement(63583..63656)
                     /locus_tag="PKI50_03290"
     tRNA            complement(63583..63656)
                     /locus_tag="PKI50_03290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63622..63624),aa:Gly,seq:ccc)
     gene            complement(63660..63744)
                     /locus_tag="PKI50_03295"
     tRNA            complement(63660..63744)
                     /locus_tag="PKI50_03295"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63708..63710),aa:Tyr,seq:gta)
     gene            64121..64894
                     /locus_tag="PKI50_03300"
     CDS             64121..64894
                     /locus_tag="PKI50_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT02771.1"
                     /translation="MNKSGENVIQSVCRAIQIIRCFEKNEELGVTEISKMAGLHKSTA
                     FGLISTLEANKLIEKNELTGKYRLGLELFRLGTKVNSALRHIAVPYLEQLVKMYQETA
                     NFVVMDDLSIIYLEKVESTQSMRISTLVGGRLPLYCTAVGKAILANLPPEELNSKLKR
                     MEFRKYTGNTVSDRNSLMKSLELTRNRGYAEDNEELEIGLSCIAAPIYNHFNIPFAAI
                     SLSGPTFRMDEIFRKEASVTLMNYTGEISMKLGYDNNNL"
     gene            64922..65773
                     /gene="panB"
                     /locus_tag="PKI50_03305"
     CDS             64922..65773
                     /gene="panB"
                     /locus_tag="PKI50_03305"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNT02772.1"
                     /translation="MAKKKSINDFYEMKKTGEKVSWITAYDFPMASFAEQAGMDMILV
                     GDSLGMVVLGYQGTVPVTMDDCISHCQAVRRGAPNTFAIGDMPFGSYQVSDEEAVRNA
                     CRFLKEADMDAIKLEGGIRVLSRIKAISDAGIVVFGHIGLTPQSSGQLGGFKAQGRTV
                     DSARELIKDALAIQEAGARALLLEAVPPELTEYLSHKLTIPVYSIGAGLPCDGQLIIC
                     GDMLGLFQAFTPKFVKKYANVAEVIINAFKEYVADVKEEKFPEDNHVYHAIDSKEAFE
                     ALFREFE"
     gene            66143..>67077
                     /locus_tag="PKI50_03310"
     CDS             66143..>67077
                     /locus_tag="PKI50_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02773.1"
                     /translation="MNSTAIKILEFDKIKELLKTYTVSNLGKDLTDKLEPSLNTDTIL
                     SWMNETTEACAILQRSGSVPIHSLNGIESIVGKLGKNMTLLPEEFEVILGLLQEVGKL
                     KRFMEDKSAIAPIVSSFALSAYELEELSEEIERCIQNGAVADKASSELSRVRKKIIIL
                     EDRIKGKLESILRSPAYARYLQDGIISSRNGRYVIPVKREYRRNVDGEVMDSSVSGST
                     VFIEPSEVRKLQDELNHCRIEEEKEVCRILSMLTNLAESYKKEITTNIQVMSYYDFLF
                     AKGRFSRSLEANPVSVNTCGRLDICGARHPLIGKSA"
BASE COUNT        16808 a        16778 c        11927 g        21564 t
ORIGIN      
        1 gtccgtgtcg cattattcta tttatatgta tttcccttta tagtataata atacatataa
       61 tacttttaag ggggtgccgg ttttgtctga ccttaacaag ttgaagaata taagcaatgt
      121 aagaaccttt aaggaaaacg aatatatatt ccatgaggga gataccggaa acgatatgta
      181 tattctcttg tccggcagag tgtcgatttt catcaaatcc aaggataatc tccaaatcaa
      241 gctgtgcgac atttgcgcag gaggtttttt cggagaaatg tccctgcttg agggggagcc
      301 ccgtactgct actgctttaa ctattgagaa cacagtagcc ctttcagtta ataaggataa
      361 ctttgaagca tttgtagttg aacagccaag tatggcattt aagctgctaa aaggcttaag
      421 ctcgagaata agggcattgg atgaagagtt aagccggtta aagaagggaa gtcaatatgt
      481 catacccccg gcggattcaa ctgatgcttt atcagacagc gccccttgca tttctgcctc
      541 caacccggaa gatttgaaag gcgtcctgta tcatgatgaa catggaactt acgaagggga
      601 atccccggat actgatactt tctatatcta tgataagaaa ataacctgtc ccatatgcgg
      661 aaaggaattc gagctgtcgg ttacatccca gtccaagctc agactcaact cgattgattt
      721 cgatttcaga catcgatatg atggatttga gcctctgtgg tataacgtcc tgatatgtcc
      781 tttctgttat tttgccggat attccaatga gtttgataat catatggaca acctctgcaa
      841 ggcattcacg gaaaaaacag cccccatccg caaaaagctg gcttttaagt attcccagcc
      901 ccgtaagctg gatgaggttt ttacggctta ctatctggct ctgcacagtg caaaagctct
      961 gagtgccggt cctcttaaaa gcgcaaggat atggcttcag ctgtcctggc tttataagga
     1021 tgccggagat gagaaaatgt atgctatcgc aacagatcaa gcttttgagc actattacga
     1081 tgtattgtac ggaagctccc tgacgaagtt gaccgacgaa caggatcaac agtgctgcct
     1141 catattggga gagctatatt tgctgaaaaa cgaccgcaat caagctgtga ggcaattcca
     1201 tgccgcaata aggaggggcg gaggaaacgc cctatacaac aaacaggccc aggacaggat
     1261 atactacgta aaacacgaaa tgaaataggc tggacgggga cagggggacg gggacacggg
     1321 gacgtttccc ctgtcccact tccagtggga cacgcgaaac gtccccatgt ccccgtcccc
     1381 ctgtccccgt cgcattctta ttctattgcc ttcttctcct cctccatata cttcacaatg
     1441 cccacgaata tgctccatgc aatcttctcc tgatagtgct cgtcatccag cagccgttcc
     1501 tcctcgcgat ttgaaagaaa tccgcattct acaagggctc ccggcatatt gttgtttttt
     1561 agaatataca tggaatcagt ggccttagcc tttctttcat tccctctgtc cagcaccttg
     1621 atcagttcat tctgtatgcc ctcggccaac ttcctggatt tttcctcatt cttcatataa
     1681 aaggtctggg ccccaaaata ttgggactgt ggaaagctgt ttaaatgtat gcttataaga
     1741 atatcagctc cgcagctgtt tatcagggca tgcctgttct tgaggtcctc gctttttctt
     1801 gtcccctgtc tgtttccgcc ttcagtatag agcccggaat tatcctctct tatcataata
     1861 gccgtgcccc ctccctgctc tatgagcctt cggagcttaa gcgcaatctt cagattaact
     1921 tcctcttctg ttataccgct gctgcccgac gctccaggat ctcttccccc atgccctgca
     1981 tctataacaa caacagtact tctaatggga agggaaaatg ccgcagtagt gcttttgccg
     2041 ataaaacata tgcttatgaa caacaccaat atccatgcaa atttgatcat atatccgaca
     2101 gccttgcttc tgtcgattat ataaattctc actgaacaca cccccaaata ctccggcaaa
     2161 acttaaaata actgttcagt ttaagatatt caccgaacca tgtacaaaag aaccgaaagt
     2221 atcatggggt tatatgggaa gggcttaaat tgagttgtgt aaattgtggg atttgattga
     2281 tgatatgctt tctctcaaca gacgtcctgc ttcaaaggct ttttgccgaa tatcctccct
     2341 gcctctgacg tgttgattat ctacatcgga gacaagcagg cttccgacaa attcaacatt
     2401 tatacattta taaagcagtt caaaaaccat gactgcacat tcaaaacggc ttttgccctc
     2461 aggctctcct gcagtgcaca caatataacc tatcctatgc ttttttgata tcaggctgcc
     2521 ctctgtcctg tacttgcttg cccatacagc ctggcacctg tcgataagct tcttcatatg
     2581 ggaagatacc gaataaaagt ataccggcga tgatgaaacc acaatatcag ctttatccat
     2641 agcttcataa acctgctgca tctcatcctt tatcacacag tatcccagct cgtagcatct
     2701 ctcgcaagcc gtacagctct ttataccctg cgaagaagca tatatccgct ctatgcttgc
     2761 ttcattgctt ccaataccct caagtatctt atcagccagc atgtcactgt tctgtccccg
     2821 cctatggctt ccatagatag ctaatacgtt tatcataata tgtatatttc ccttcctgat
     2881 attagtgcat gtgtgctgct cgactctcgt atctcgcacc tcgtgcctcg tacctgaccc
     2941 tgaagcatga ccctttagcc ctttatatat cctttccctt taagcttctt tatatggctc
     3001 tcaaccttct cctgtatatc tatcgaatag ctttcttcca gaacaaacat cattgaaatc
     3061 gccacctggg ccacatccag cagctcttca gctattctgt caacgcattg tctttcttcc
     3121 atcctgcatt tttctccgtt caaccctctg tactttccga taagctgtgc cagttcacct
     3181 gcctcttcca tcagcttaag ggctgtggac tctatattgg gtttaaggtt attaagcctc
     3241 ggcaggctga tgctcttgta ttcggtatct ccaagtctgt tcattaagaa ttcctccgta
     3301 aatattttgt taacaactta tatgtatttt ttgcttagcc gcagtttcat aaaactgttt
     3361 acgtttcttt tttaactgtt ctttaaaagt gaacatggat attgccttaa aatcttctat
     3421 tctcatatta acaaatttgc agcccatttg aaactttttt tcggagggta attcattaac
     3481 tcttagaact ttagctgtac aatcaataaa ataattagtc ttgagcttga aactgaaaac
     3541 cgagtctacc gggagacata aatttgtctc tgccaatacc cctcctgcgc tgatattttt
     3601 tatcgttccc aaaaatgtct tccctaactt tccaacagag acattattta tagttaccgg
     3661 tctggctaca aagccaattt cacaatctac cctggcaaat cttctttttt ctattgtatt
     3721 taatttattt tctattggtt tttttattat aatctcattg ccttcaagat aggttatttc
     3781 actgtaaaac tcatatatat catcattttc aagaaaaata cacaatatcc cccatccgtt
     3841 tctcaaaaaa ccggcaggat tcacactcag aatcagtgag tcttcagtaa tagaatttat
     3901 atacccatat gcttcagaat cggtaaatgg tgacaatata acaagaaact gcccatttct
     3961 aaactccata ataagcttac ctcctcccgt ttaagagcaa ctactaatta gtattaagta
     4021 aaactttttt tactattata tcaaatttca atataattcg acatttaatt gctataatct
     4081 gtcattttca ttatatgttt ggctcccaac caaacaaaca acaattcatc cggctctctt
     4141 atacaacgct gaaaactcgt atctccaatc aaaacatgca aaaagagaag gcatatatgc
     4201 cttctccaat tccgtaacaa taattatctc tttgagaatt gaggtgctct tcttgctttt
     4261 ttgagcccgt atttctttct ttccttcatt ctaggatctc ttgtaaggaa tcctgctttt
     4321 ttaagcatag gcctgagtgt atcgtcagct tccaaaagcg ccctcgaaat gccatgcctt
     4381 atggctccgg cctggcctgt aaagccgccg ccgaaaacat ttaccaatac attgaatttt
     4441 gaaagggtat ccgtaagaat cagaggcagc agataatgca ctaacccaat ggtagttgaa
     4501 gtatttgtat atagtatttg tataggttgc tcaatgaatc cgatgttttt cagcaaagtg
     4561 tttattatgc cggtgtttcc cagtacgata aaccaggaat aagcacgaat cagatagccg
     4621 gtccagaacg gcgcaatgat cagcaccaga aagaagttgc gccagcgttt cgggactgcg
     4681 taatacaaca ccattgccag aggataggca acaagtgtag taatagtcac aactttaagg
     4741 gaaagtaaaa gggaattctt caacgccttc caatacataa cactggtgaa aatcctctca
     4801 tagctttgga aggtaaattc gctggtaagc ttataatttc ccatggtttt aaaacttgtt
     4861 gcaacgatga atagaagagg aataagatag aaaaatccca tccagaatga aatcggcagg
     4921 caccaaagag cggagcggtt tcctcccgga cttgcattgc ttgcattatt tttctttttc
     4981 attttaacaa cacctcccag tctatgcctg cgccggcagt accagcgcat cctgtgcaga
     5041 aaatgaaagg gttacccggt ctcccggttg taaagcagat ttccctataa catccgacag
     5101 gaaggtttca tctccttcgt atctgagcat ccagcgcacc cagaggttca tccaacagca
     5161 tgacaacagg atgcaacacc aaggatcgtg ccaaggctat acgctgacgc tgaccgccgg
     5221 aaagttgact gggtttcctc ttttcgtatc cctggggccc taccatattc agtgcatgaa
     5281 ggattctttc cctcgctttt ttatgctcta taaacataca tccaattttt ctatcattta
     5341 ggcacctttc caaagcaaag agaggctggc agtgccttcc ccctttgttt ttcaaaagaa
     5401 tatataagta tacatagatg tccctacttg cttttgaatt cgtcccagat agcctgaacc
     5461 tcaggagtcg taagatactg tatgaatgcc ttggctgctt ccttgtttta ctggccttta
     5521 cgatcgaaat tgattctgtc catgaaacgc cgccttcctt gggcactgta acttcgatgg
     5581 gcttgccttc ttctttcaga agaacctgga gccactcacc cccgaagctg atccatgcac
     5641 tttcattggc aagagcttga attgtgtctg cattggtcgc agcaatcata ccaacctgcg
     5701 gtttcagctg gttcaaggta tccttaactt ttgaaagttc atcccctgaa atgtcgtagg
     5761 gttcatcata gcctgcatat cgggaaagca ttcccatatt cggcaggtac cagtcaaaca
     5821 gaacaacttt gccggctgcc ttagggttgt ataaaaatgc agtggatgag gcctcctcct
     5881 ctgtaatata attggagttg tatacgattc cataatagcc ccaacggctg acaactgcgt
     5941 acagcttatc cccaaccttc acctggtcaa aatccttcca gcggtcatag aaattatcaa
     6001 tttccggata atcttcggat tcagctcatc aatcaggtct gcatccacca gtttggctac
     6061 ccaaggtgta tccggagtaa caatatccca ttccccctct gccgaattgg caagaagtga
     6121 aaacatctga tcggatgtag aatatacctt tgcattaatt ttgacaccgt atttttcctc
     6181 aaagggtttt attatctttt cttcctcata gcctggcatc acaagcaaat tcaactcacc
     6241 gcccagcttt ccgccttcct cggccggtgc cggctccacg ggtttgttct cctagcatcc
     6301 gcttaatacc ggcagcagca tcgtcagtaa tactacaaaa ccaacgcctt tttcattatt
     6361 tcttcctccc atactgaata ttttgctgtt cctttctcta cgccgtaaca attatcttgc
     6421 tgtatataat actttcaagt gcttttcgac actataccaa gcgtcagagt ttgaactata
     6481 tgtatacata tactaccgta tgcatatata tgtcaggagg atattatctg tattattatt
     6541 atttttcctt tgccgcccat gggcatttcc cgaaggaaac ctcggtgcga gaagcatact
     6601 tttctatatt atacaaagtc cctcaggcac aattgcctga aggactttgt attttgcaaa
     6661 ccctaacgtt ttgagaattg agacggggca gcggggcacc aaggcaggta tctcgttaca
     6721 acctgaaaat ttcaaagtag ttctttttaa atgtaattag cccttccttt tccatccgct
     6781 tcaattcatg agaaagcacg ctgcgattca tacctaagaa gttggctaac ttttcacgat
     6841 caaacttgat atagaattga ttgctcccat tgattttctg ttgcaaagct aagtaatgta
     6901 caatacgctc cctaatacct tttatggaaa ttgcttcaat tttatgatac ttacgcatgt
     6961 tttcatcagc aatatagagc atgatattcc gtagtagaat ttctctgatt ccatcgtcaa
     7021 ttaatccatt tttagttatt ttctcaatag aaaaccaata tatatccgta tctttctcgc
     7081 aataaatagt ataaggggcg ttttgttttc tggtaaatgc gacttcagca caaacaagat
     7141 agaatggaac aagtttttgc atgagaagtt ccttaccatc catcgtgaac tttgaaatat
     7201 atgcatttcc ctgaagaagt attccaatcc ggtttacgat tgcatcttcc tcaacaatca
     7261 cctcggcttc ccggtacgaa ttaattacgc catctaactg ttgttttgcc aatttgatct
     7321 gtcggtctga taagccggaa aataatctgc aattttgcaa atcttacacc cctttcccaa
     7381 cttactccgt atgccgtcgg caatctgccg acacttttct ttgcagagtc aatacataat
     7441 atagcatagg ttacccccaa aactcaagaa aagtttgcaa tatttacctg tttcaaaata
     7501 tgaaaactac cccactaaag tttcggtgct cctgcggggt cattcttttg tagttgtgac
     7561 ttactcaatg atctcggaaa tttgctgtgg agtaagatca tatttaaaga aatggctgta
     7621 aaaatagctt actttttctg ccagcatgtt atcagaaacc agctcgggat aaagcttgtt
     7681 gagcgtccag aaaacggtta gaggctgctc taccgtacgg ttgccccaag tgtgggcaac
     7741 tgtggggata taatagatgc tacccgcctt tacagcagtt acatctgtat aaagcttatc
     7801 ttctttgatc ttatcaatca tgtcacgctc ggtgacgatt attatatccg gatcccacag
     7861 caatagctcc tcttgagtat attcacggga ttgaccggtg gttttaccgt cgtttgttac
     7921 acttatgccg cctgcgacat ctatccacca ctcagcaata acatgtggct gggtgagatt
     7981 ggtaacattg ccatagagaa cggttttctt gtcttcatcg gtgagcttgg cggtcaaatc
     8041 ggaagcttct tgcaccatag aatcaaaata agccaaatag tcctcagcga tatcttcttt
     8101 tttaaggatc tcgcccatca gattgacggc agccttcaca tcttcagtct gtttccattc
     8161 cagatatata caggggatac cgttttcacc aagctgttct gcagtttcct tggtcatagt
     8221 aatacaaaga tcgggttgaa gctttagaac ctcctcaagg agcagttcgt cgttactgtt
     8281 agccagcagt ggtgcgtcct taatctgcgg cgcaaactca tactgcattt tccatttgtc
     8341 ggatttggtg aatcttgccg gcatctggtt gcacagcaga tcaccactgc ccatcagctc
     8401 tacaaaagca ttaagtacgc caactgagcc gaatgtagcg atagacttga tctcgccggg
     8461 aatcgtcacc tggcggccgg ccatatcagt cacagtaaaa tcggtaatga catccggctg
     8521 cgagttggcg ggcgcacaac cggagaatgc gaccatcaac ataataatgg ccatagtaag
     8581 tgctaataaa cgtttcatct taatcctcct taattgtaag atatataatc gcacacatcc
     8641 tgtcgttagg acatggatgg aatgcaaact ttcgattctt gcgtacctga attaaaaaca
     8701 gcctgagcgt ctgctacgta tacatctaac ccgtatagtg aagataggtt ccgtgacgta
     8761 ataatgtctt caggggttcc gtcagcaaaa acaatgccat ctttgagcat gagtacccgc
     8821 gtggcagcta gaaaagcctg atccggcaag tgtgaagtca tgagaatagc ataaccatcc
     8881 tccgcaagtt gtctgatcac ctgcaagata cggatttgat tcgcatagtc gaggctggcg
     8941 gtaggctcat ctaaaataag gcaatccgtc tcctgtgcaa gagcacgtgc tataagaaca
     9001 agctggcgtt caccaccgct tagctcgtgg aaacggcact gcgccatgca caatacattg
     9061 agccgttcca tggcagcttc ggcagctatt ctgtctttaa gggaaacaga tgcccctaac
     9121 ttctgatgtg ccacacggcc catcaaaact gtttcccatg cagtgtaggg aaaagtaagt
     9181 gccgacgctt gcggtacata ggagatcagt cgggcgcgga ctctcgggcg taaagaagca
     9241 gcatctttcc catctataaa aatttttcct ccctgtatgc tatgcagatt aagcaaacag
     9301 cgaaacagtg tagtctttcc ggtaccgttc ggtcctaata cgcaaagact ttcacctctc
     9361 tcaaagttga aggacacgtc ccgtagtacc ggtgcactgt gccgatagct aaatatcaca
     9421 ttctcgactc taatcatgtc accaccccct tttaatccgg gccagcatta cggcaaaagc
     9481 aggagtgccg atgagagctg tcataacacc aagaggcaat tccactcctg ggataccccg
     9541 gatgatatta tcaatcagca gtaaaaaaag tgcgccgatc agaaatgaaa gggaacacaa
     9601 tctcccgtaa ctcgcacctg tgagtgcacg ggcaatgtga ggaactacca ttcctaccca
     9661 accgatgata ccgcatatgc tcacagaaac taccgtgagc aatgtggaac tgacgataac
     9721 cacaattttc accaaaggca cattgacacc catgcttaag gcttcatcct ctcctgcaga
     9781 cagtgcgtcg atatgtgatc gaaaaagaaa taaaacaaaa gcggaagctg tcaccaccgg
     9841 gagcattatg agcacgtcat cattgttacc ctttccaagg ctccccatca accagaatgt
     9901 tattgaagga agttggttat cagtatcggc taaggttttg acaactgaaa gcagagattg
     9961 gaatagactg gacgttacaa tacctgccag tatgagaacc gttaaattct gtcgtccacc
    10021 actgagggat gcgattaggt acgcaagccc tacagccaaa agaccaaatg taagtgcaaa
    10081 aaactgtata ccagcccatg agccgttttg aagcatagct aaagccgcac caaagcctgc
    10141 gcctgccgat acgcccaaaa tatccggcga gaccatagga ttcttaaaca atgtttgata
    10201 agacgctcct gccatggaaa gggatccgcc cacaaggatg gccagcagga tgcggggcaa
    10261 gcgcacatgc agcaccacct tttcaagtgt accctcccag tatgccgtta tggtaaaaaa
    10321 gcgcgacagg ataatatctg ccaccgttct aatagcgatt ggataccgac cgataaggaa
    10381 tgagatgaaa aaggcggcaa ccaacccgca taggaacatg accatcatca gagggtaacg
    10441 aatctgtttc ttcatggctg attcttaaat atcacggttc tctctgccga tttgccgaag
    10501 aaatgatatc cggaaccccc ttcggatatg atatctaaaa gctcgtccgc ctttgtcaca
    10561 aggatgccgc ccaccatggt aactccccgt gagaacagtg catccggcag catgctggct
    10621 gtgggaccgg ttacgataat ctgtgcgccg ggcttagcca tattcaatat gccgggaagg
    10681 gtatcattga gaatggtcac gccggtaatg acgagaaggt cggcatccgg cacatactta
    10741 tgtgcatcct cagcgggagc gtaataaggc agctcaccgg atttaagcgt gcggggatcc
    10801 tgttcgagaa cgcgaaaatc acagcctccg gttatcagct ttttgagcat aggtacaagt
    10861 gcgccgatga caacggactt tttatattgt gaaacatcca cattatcaaa tgcatccata
    10921 ccgattgcaa tagaatatcc ttccggcggc tccaactccc atatcaagtt gctcaatgca
    10981 ttgagagcag caatgcccag agtctttttg agaatgttgt cactgaacac atcttccaaa
    11041 tattgctctg cactgcgtcc ccgcagtttt cctgacaacg gcatggcagc tgccgaactg
    11101 gggcagcata ccgctgcagg gatatcctta acaggtgtga agcatagccc cccatgtccg
    11161 gtggacagct tgaccccgga gaagaaaaga cccaataccg ccctatctat ggtaatattc
    11221 gaaagttctg attttagacg cccttttact atatgtacag attcaagtaa tatttcattt
    11281 ttcgacatga cgtattcctc cttgtgcatt gtcatgatta gatatagctg aactcgttct
    11341 gttttctctg caatccatta cccgtatttc aaatatcaaa gccactcagc aactctgcac
    11401 gcaccatttc tcctgcttca aaagtctcct tgccaatggg agcaataaac agtgcattac
    11461 aatctgccaa cagttgtgcc aagcccttct gggggctgag aacctcagca gtataccggc
    11521 catttataca tttcactcgc agccgtacat acatctccaa atgcgatggc ttttgcactt
    11581 tttgcttcag aacagccctg acggttctgc gtaaaggcat gtcctgcttc agcatatgga
    11641 aaaccattgc cttaacgcac caatccatag catagtagga tgcgagggta ggccccggca
    11701 ggttgatgac aggctttttg tcaatgatag ccaatgcaat cgggatacct gggatacaac
    11761 gtataccatg ctgaaaccat tcggcatgtt tttgcagcag acggctggcg taatcttcgc
    11821 tgcccataga tgatcctccg tttattaaga cgatatcggc ttttgagcat gcattcagca
    11881 gtgtttcttc catgagtacg ttatcatcct taacgatagg gcagcagtgc gcctttgcac
    11941 cccattgcgc aagcatggtc cgtaccatta gactgttgct ttcgatattt tggcctcgct
    12001 tcggagacac gccaatggga atcaattcat tgcctgtagg aatgaatgag acaacaggtt
    12061 ttttataaac ctttaccgta ctaaccccgg cagtgctcag gaaagccaaa tgtacaggct
    12121 gcaaaagggt gtcctttttc agcagctgct gcccttcctt caatgtatcc ccgcgcggcc
    12181 taatattttg tccctgtttt attggttcct tggaagtaat ggttagcgat ccatccgaat
    12241 gtcttacgat tctttcaacg gcaatcaccg tatcgaaggc atcatcaaaa tcatcgccgg
    12301 tatccgctga tacatgggca attgtgcctg tccaggggaa ttgaccccca ctggcttgca
    12361 ttagcgcctc aaaggataca gcgatgccgt caagcttgga actcctgcat accggcaaag
    12421 tattcacaga actgacagca tcggaaagca cacgtccgac tgcttccgat accgcaatgc
    12481 tctctgtggc tatctccgga ttccaacgtc caaaaagctc cttaagtgcc tcagagcgca
    12541 taagaaattg cttctgattc aggtacatat taaaaacctc ctgtcccggt actttattgc
    12601 ttatatggtt ttatttcctc agattagtga ccacacaaag actttattat cattatctac
    12661 taaatcctct ataatgtaag ttgttataac aacattttta atgatccggg gaggtgttaa
    12721 cattgagcgg attcgcaaat ccggtacggg cagggacagg tgggacaagg tgcctgtcca
    12781 tctgcccaat aaaaaaacgc cagaccgttt ccaatctgac gcttgtgttt tgaaattatc
    12841 tctttgagaa ttgaggtgct cttcttgctt ttttgagccc gtatttcttt ctttccttca
    12901 ttctaggatc tcttgtaagg aatcctgctt ttttaagcat aggcctgagt gtatcgtcag
    12961 cttccaaaag cgccctcgaa atgccatgcc ttatggctcc ggcctggcct gtaaagccgc
    13021 cgccgataac atttaccaat acattgaatt ttgaaagggt atccgtaaga atcagaggct
    13081 gtcgcacaat aagcttcaat gtctctaatc caaaatagtc atctatttct ctatcattaa
    13141 ttattatctt accttctccg ggaaccagtc tgactctagc aacagactgc tttcttcttc
    13201 ctgttccaaa atactgaact ttctttgcca cagtaacttc ctcctttcaa acctatatgt
    13261 ccagttcaag agcttccggc atctgagctg cgttttcatg ttcggcgcct ttataaactc
    13321 ttaacttttt aagcattttt ctgcccaagc tgtttttagg aagcatgcct ttaactgctt
    13381 catatacggc cttgtcagac tgttcagcca tgaactttct gtatggagtc tccttcatgc
    13441 tgccgacacg ggttgttact gtcctgtgca tcttctggtc aagcttcttg cctgtaagta
    13501 caaccttgtc gcagtttata actattacgt aatcgcccgt atcaatatga ggcgtataaa
    13561 tcggtttatt ttttcctgtt aatatctttg caacttcact tgcaagtctt ccgagaacct
    13621 tatcggttgc atcaactaca taccattttc tctgaatctc atcggtcttt ggtatatatg
    13681 acttcatttt ttccactcct tgtctataat attatgacag atgtgaactt tacagcatgc
    13741 aattgtgttc cacattcaca aaagccggct gccgattcgt tcaaataagc acactaaatt
    13801 tttctatttt tcaagtttat ttattctgcc aatattaata cattgcaagt gattggggct
    13861 aagaatcact tttcgcaaca tataatattt taatacattg aaccacgtgt gtcaagacaa
    13921 attatagtat acctcttcca aatacagacc ccgtgccgga acggttatac cagcctgttc
    13981 cctgttcttt gacagtatta cgtccttcat atcctccgga ttgatttttc caattccggc
    14041 atacagcagg gtgcctgtta ttatacgaat catattgtac aaaaatccgc tgcccttgta
    14101 aataaaacga attaattccc cttcctgctc tatggaaata tcatatattg tcctgacagt
    14161 attggaaacc ttgctgcccg ctgccatgaa cccggaaaaa tcataagtac ctataaaagc
    14221 ttctgccgca gtctttacct tgtctatatc taatctttca caatagttga catgatatgc
    14281 atgatttcta agaagcgcag aaggaaatct gctgtttata acgagataac tgtacttttt
    14341 tccgacagcg ctaaatcttg catgaaaatc aaggggcact tctcccgatt cctttataac
    14401 aatgtcttcc ggcagcttgt tgttcaaagc ataggcaaac ttctccggag gtatggaact
    14461 ttccgtgata aagcttgccg cctgccctct ggcatgaact cccgcatccg tcctgccgga
    14521 gccgtttatg cttattgcct caccggttac ggaaagcaga gccttttcaa tttcagcctg
    14581 aatggaaggc ccattctcct gaacctgcca tccgcagtaa ttcgtcccat catattcgat
    14641 tatcagttta atatttctca tacacatccc ccgcgacctc gcaaccccga aaccccgaaa
    14701 cctgcgtttt acctgctcca tatcaccaga aagcaaaaaa ctgctataat catcatggca
    14761 ctgtaatctc tgaattccag cttcagctgc ttcattctcg tccttttctc tccgcccctg
    14821 tagcaccttg cctccattgc aagagcaagc tcatcggccc tcctgaatgc gctgatgaat
    14881 aacggaacca acagcggaac cattgcttta gccctattta tcagattgcc cgtctcaaaa
    14941 tccgcacctc ttgccatttg cgccttcata atcttgtctg tttcctcaag aagggtagga
    15001 ataaatctca aggcaatggt catcatcatg gccagctcat gagcaggtac tccgattctc
    15061 tttaaaggat tcagcagctt ctcaattcca tcggttaaag ctatcggcga tgttgtcagt
    15121 gtcaaaagag aggctccggt tatgaggaat atcagcctca gccccataaa tcccgcttga
    15181 tacaaacctt ccttggtgat tgaaagcccc cagatcctgt atattacttc tccttgtgtc
    15241 ataaaagcat tcaatatcac cgtcaagaca attataacaa tcaacggctt caggcccttg
    15301 gcaataaact tcagggatat ctttgaaagc ccaatagcca aacccatgaa cgcaataata
    15361 taaaggtaag aaaagtaatt cccgactaaa aacaataatg ttataaagac aaaggttata
    15421 attattttta tcctgggatc caggttatgg acggctgtat ctccgggaat gtattgtcct
    15481 attgtaatgt ccttaatcat attctattac ctcttaaaaa gcttcagcag ctcatttttt
    15541 gcttcatcta tatttatgca atcagcaatg gggtacccct tttttctgag ttctcttacc
    15601 aggtatgtca tttgaggaac acccagcccg attttttcca aggtttctgc ttctccgaaa
    15661 acatttcttg gtgtatcaaa gtatgctatt ttgcctttat tcataaccaa taccttgtcc
    15721 accaggttag caatatcttc catgctgtgc gacaccagca ctactgttat tttctgcgtc
    15781 tcatgaagtg tcttgaaccc cttgagtata tcatcccttc ccttggggtc caaacctgcg
    15841 gcaggttcat ccaatatcag cacttttggc tccatcgcca gcactcccgc tattgcaacc
    15901 cttctctttt gccctccgct gagttcaaag ggcgatacat tgcttacgct gtcaaaatcc
    15961 aatcctacaa gctcaattgc tttacggact cttgcatcaa cctcatcttc acttaaaccc
    16021 aggttccggg ggccgaaagc cacgtcttta tacaccgtct cttcaaaaag ttggtactcg
    16081 gggtattgga aaaccaaacc taccttctgt ctgattttct tcatctgtac ttccttggag
    16141 cccagttctt ccccatcgat gaataccttt ccgctgctgg gcttcaatag tccgtttaaa
    16201 tgctgtatga gtgtcgactt ccctgagccc gtatggccaa taaggcctat gaattccccg
    16261 tcttgtatct caaagcttat attgtctatt gcaacatgtt cgaatacaga gtctttcata
    16321 tatatatggg taaggttctc tactttaacc ggcacaattc caccaccatt tcttcaacag
    16381 tcagaatatc cggagaaagc tgcagcccct ccctgttcag cctgtgactc agttctgtaa
    16441 cctgaggaac atcaagtcct gcggctttta ccgtttccac ttccttgaat acatccttag
    16501 gctttccttc cataataatc ttgccgtttt tcattacata tatcctgtcc gcctgtatgg
    16561 cttcatccat gtagtgggta atgaggatta tggttatacc ttcctcctta ttgagcttta
    16621 taatggtatt cagcacttcc ttccttccaa ccggatcaag aagtgcagta ggttcatcaa
    16681 gtatgatgca ttccggcttc atggcaataa tccctgctat ggccactctc tgcttctggc
    16741 ctcccgacag caggtgcggg ctgtgcttgg catattcggt catatctacc gtttttattg
    16801 agtcgtctac ccttcttctt atttccaaag gcgggatccc aagattctca ggcccgaagg
    16861 ctacatcttc ttcaactatg gtagctacta tctggttgtc agggttctga aacaccattc
    16921 ccgcagtctg tctgatatcc cataagagct tttcattctt ggtgttcatt tcttttacgt
    16981 atacagtacc tgctccgggc agcagcaggg cattaaagtg ttttgccagg gtagacttac
    17041 ctgacccatt gtgcccaagt atagccacaa actcaccctt ggcaatatta atatttatat
    17101 catccaaagc cggtttggcg cttccgctgt tagtcccgta ttcaaatctt acgttttcag
    17161 tggtaatcat atttgccatt tttaattcct tctttttctt ccgagcagtc gtttagtctg
    17221 cccaacctat agatattctt cgaatccagt ctttaacccc tcgtgtcccg aacctcgaac
    17281 ctcgtgccgg cgttttgcgc acaaaacgcc tcatagcaaa aggggactaa gcagtcactt
    17341 aatccctaat aattcgatat gatctacgct tctacaccag ttccagtaca actacttccg
    17401 ctgcatcgcc gcgtctcgga gcaaccttcg ctattcttgt atatccgccg tttctctcct
    17461 tgtacttcgg cgctatatca tcaaagagtt tttttacaac atcttctttt gtaatatatc
    17521 ccaaggcctg acgtcttgca tgcaggtcac cgcgcttgcc aagtgtaatc attttttcgg
    17581 caatgctctg agtttccttc gctctggtct cggtagtcac aatctttcca tgctcaagaa
    17641 aagaagtaac caagttccta agcatggctt tcctatgatc tgaaggacgt cccagctttc
    17701 tgtatccagc cattgcaata cctccttttg ttttgtattg ttaatcaata tccgtattaa
    17761 actgcttacg caccagaatc ctagtcctcg ctcgttttta agcttaatcc caaggcttca
    17821 agcttctgct gcacttcttc caatgatttc ttacccagat ttcttacctt catcatatct
    17881 tcctctgtcc tctgggtcag ctcctcaact gtatttattc cggctctctt caggcagtta
    17941 aaggatctga ccgaaagatc cagctcttca atggtcattt caagaacctt ttccttcttg
    18001 tcttcttctt tttccaccat tatttcaaca ttgccaacat gctcagtcaa ggttatgaat
    18061 aacttcaagt gctcgctgag tattttggca ccaaagctta ttgcctcatc cgggttgatt
    18121 gtaccgtctg tccagacctc aagcactaat ttgtcaaaat cagtaacctg tccgactctt
    18181 gtattctcta ccagatagtt gacctttctg accggcgtga aaatcgaatc tatcgggatt
    18241 attccgattg gctgtcccgg ctgtttgttc ttttccgata tctgatatcc tctgcccatt
    18301 tccaccgtca tttccatata taacctatgg ccttcatcaa ccgttgctat atgaagatcc
    18361 ttgttaataa tttcaacatc ggcatcacat atgatatcgg aagctttaat ttccttttca
    18421 ccctgaacat ctattttgat aatcttggta ttttccccgt gcatcttaag cgcaagattc
    18481 ttgatgttta atattatttc cgtcacgtct tccgctacac cgggaattgt tgaaaactca
    18541 tgaagcacac cgtcaatctt aatggaagtt acagcaactc ccggaagtga cgaaatcaat
    18601 accctcctta aagagtttcc aagggtagta ccgtaacctc tttccagagg ctccactacg
    18661 aacttcccga aagtataatc ctccgacatt tcgatacatt caattttggg cttttctatt
    18721 tcaatcatcc tacaaaccct ccttttatct atggcttttt tattctgagg gcaactgata
    18781 cgtcaagttt atataaagaa acttcatctt ctatcgctta tgctgcacta aaacttatta
    18841 tcttgaatac aactcgacta ttagatgctc ttctatcgga aggtcaatat cctctctggc
    18901 cggaagagca actattcttg cttccatcat ctccgtattt atagataacc actgtggagc
    18961 agttttccct gctgcgtttt ccgcaagtcc cttgaacttg tcagtactct tgctcccggg
    19021 ttttacagct atcagatcgt taagacctgt ctgataggat ggaatatcta ccttcctgcc
    19081 gttaactgta aaatggccat gtctaaccaa ttgtctggct tcggctcttg attcaccgaa
    19141 tcccattctg taagccacat tgtcaagtct gagctcaaga agtctcaaaa ggttctcacc
    19201 tgttacaccc ttctgtcttt cagcttcaac aaagtagtct ctgaattgct tctcaagtat
    19261 tccgtatatt cttcttactt tttgtttttc tctaagctgt attccatact ctgacacctt
    19321 tttcctgccc tgtccgtgct gacccggagc gtatgccctt cttgcaacag cacatttatc
    19381 cgtataacag cggtcaccct tcaaatacag cttggatcct tctcttctgc aaagcctgca
    19441 cttagcgtct gtatatcttg ccatctatca cacctcctca ataactaaac tcttctgcgt
    19501 tttggcggtc tgcaaccgtt gtgaggtata ggagtaacat cttttataag gctgacttcc
    19561 agtcctgcag cctgaagtga tcttatagct gcttctctgc ctgcgccagg tccttttacg
    19621 aatacctcta ttatttttag accatgttcc attgcacctt ttgcagcgac ttcggctgcc
    19681 atctgtgcag caaagggtgt gctcttcttt gatcctctaa agccaagtga accggcactg
    19741 gaccaagaca atgcattccc tgtgctgtct gtcagggtaa ctatggtatt gttaaaagta
    19801 gactgtatat gtgcagctcc tctttcaaca ttttttcttt cttttcttct tctggttttc
    19861 ttagcctttg gctttgttac cattaaagca aacctcctta tttaccaatt tactttcttc
    19921 ttacaccaac agtcttcttc ggacctttcc ttgtcctcgc atttgtcttg gttctctgcc
    19981 ctcttacagg catacctctt ctgtggcgta atcccctgta gcatcctatt tccattaatc
    20041 ttttgatatt taaagctata tctctcttaa ggtcaccttc aactttaacg cttctgtcga
    20101 ttacttccct taacctgcct atttctgctt cagtaagatc ctttattctt gtgtcaggac
    20161 ttacacctgc ttctgccaaa attctgtttg atgttgctct tccaatgcca aatatatatg
    20221 tgagcccaat ttctgctctt ttgtctctcg gtaagtcaac accggcaatt ctcgccatta
    20281 gtacttgcac ctcctaattg ctttttgttt atactaaaat caatcgggaa cacccattta
    20341 tcatgggcca gccgcgtccc ccgaactggt tttatccttg tttctgttta tgtctaggat
    20401 tttcacagat aaccattact ttgccttttc ttctgattat cttacacttt tcacatatag
    20461 gctttactga tggtctgact ttcatcttaa accctccctg tatcatttgc ctcgccaagt
    20521 gatcctgcct ctggtaagat cataaggcga taactctatt gtaactttat ctccgggcaa
    20581 aatcttaata aaattcattc tgagcttgcc ggatatatgt gctaaaattt tatgaccgtt
    20641 ttccagctct acctgaaaca ttgcatttgg taatgcctca actacttttc cttctacctc
    20701 tattacatcc tgctttgaca taaggtaatc aaacctcccg ttcccgatat caatcaatag
    20761 attttaacaa tttcctgatc tcgctgttac tgagtttctc acaagattcc agtttttctt
    20821 taatattctc ggcaacatga ttcagctttt ttagatgctt tactttctta agcttaggct
    20881 tatctacttt tcttatgtcc ccatcagaaa tatagacata cttttcatca attatacata
    20941 aaacaactaa tttgcagcct ttatcccgtc ctgcggctga tattactatc tgtcccaaac
    21001 ttatatcctg catatattca ccttccgaac agctacaaaa gcgtcagtat ttcaggcttg
    21061 ccatttgtaa tgacaatcgt attctcataa tgtgccgatg gttttccgtc agccgttacc
    21121 actgtccaac cgtcttcccg gacctttact gcatatttgc cctgatttac catgggctca
    21181 attgcaagcg ccataccggc cgctaatctg ggtcccttgt gcggcagccc gtaattaggt
    21241 atctgcggat cttcatgcat gtttcttcct attccatgcc cgacaaaatc cctgactaca
    21301 gaaaatccgt ttgactccac gtgtttttgt atgctatggg aaatatcaga caatcggtta
    21361 cctggcagag cgtattcaat ccctttgaaa aaactctctt cagttacctt taaaagcctt
    21421 tccgtttctt tatcaatgct ccctatccag taggtcctgg ctgcatcccc ataatatccg
    21481 tcataaacgg ctccgatatc tatgcctatt atatctccgt ccttcaacgc tgtaggcccc
    21541 ggtattccgt gtacgacctg ctcatttatt gatgaacata tgctggctgg aaatccgtta
    21601 taatctttga atgcaggtat tgctcctctt ttccttataa actcttctgc aattgtatca
    21661 agttccaatg tagtaatacc cggtctgatt gccttactca aaagctcatg ggtttccgca
    21721 actatcctgc ccgctattcg catcaactcg atttctctgg gagactttat gctaatcatt
    21781 taaatctctc caataccctg cagatgtctc catatacttt atcggcaggc tgttcaccat
    21841 ttacagaaat cagaattcct tttcctttgt aatattcaat aagagggaat gtctggtttt
    21901 tatatacatt caaacggttc atgactgttt cgggcttatc atcttccctg atataaagct
    21961 tccctccgca cttgtcgcaa attccatccg ccgcgggctt taaaaagcat agattatagc
    22021 tttctccgca atcactgcat attctcctgc cggccattct ttcaataatt gtttcggcgg
    22081 atacttccag gttcacaact gcgtcaagtt tttttcccat tttttcaagg tccttgtcta
    22141 aagcttcagc ctgagctatg tttctcggaa aaccgtccaa caggaatcct tcattgcagt
    22201 cctcttcaag aagcctgctc tcaacaatgc ttacagtcaa actgtccgga accaataaac
    22261 ctctctcaat aaacttcttt gcttctgctc cgatggctgt atttctgctg atattgtctc
    22321 tgaatatatc cccggtggat atgtgcggta ttccaaactc cttttctata aagctcgctt
    22381 gtgtaccttt gccggatccc ggcgaaccca acagcactat cctcatttct acctctccaa
    22441 tccggagttg tgcagaattt tacacaactg cgttcaatat aaacctaatt tattttaaga
    22501 atccctggta attcctcatc accatctgcg cttccacctg ctttacgaaa tccattgcaa
    22561 caccgacaac tatgagcagt gaagtgcttc cgaaatacac cttgatacct gtaaagttca
    22621 taaggaatat cggaactata gctattacag cgaggaaaaa tgctcctacc aacgtaattc
    22681 tgttaagtga ttttgttata aaatcggagg tcggttttcc cggcctgatt cccggtataa
    22741 atccgccata cttcttgata ttgttagcga cctctatagg attaaaggat actgctgtat
    22801 agaagtatgt gaatccaata atcagcaatg catatagtat gctgtgagcc caggatcccc
    22861 aacccaaata tgtgttgaaa aacctgtatg cccaggaatt cggaaagaat tgtgcaattt
    22921 gtcccgggaa tgctattaat gacattgcaa atataaccgg tattactccg gcttgattta
    22981 ccttaagcgg aatatgagtg ctctgtcccc catacatcct tctgccgaca acccgctttg
    23041 catactgtac cggtattctt ctttcaccct cctgaataaa tattacagcc attattatca
    23101 atatcgcaaa tatcaggaag aaggcgatct caataagatt ggttgatttt accttgtaga
    23161 gttcaaaaat tttcagagct ccgccgggca ctcttgagat tataccggca aaaatcaaaa
    23221 gagatatacc gtttccgata cctttttcat ttatctgctc gcccagccac atcaggaagg
    23281 ctgttccggc tgtgagggaa agtgctacca atataacaga tgcagcatct gttctcagaa
    23341 gataccctct gagtccataa gacaaagccg ccgcctgaat tagtgcaagg acaaccgtgc
    23401 cgtatcttgt gtactgtgct atttttcttc ttccttcttc cccttcttta gccagggctt
    23461 ccaggctcgg aattgcaatt gtaagaagct gcattataat agttgagtta atataaggtg
    23521 ttatgctcat tgcgaatatt gcgaagttat caaatgctcc acctgaaata gtgttaaaaa
    23581 atccgagcaa accgccgctc tgaataagcc gtgacagagc ttcggtattc ataccaggta
    23641 caggaatgaa gcttcccagt ctgaatatta taagcattac aagtgtaaaa agcatttttt
    23701 gtctcagatc aggaatcttc caagcatttc ttaatgtgtt aaacacccta aatcacctct
    23761 acctttcctc ctgctgctgc aatcttttct gcagctgttt tgctgaattt atgtgcctga
    23821 accgtcaagt tttttgtaag atcgccattg ccaagtattt taacaccggc aagcatagct
    23881 tttaccaagc ctgcttcttt taatacctcc ggatttacaa ttgttccgtt atcaaatctt
    23941 tcaaggtcgc tgaggttaac aacagcatat tcttttctga atacattggt gaaaccaatc
    24001 tttggcagcc ttctgtacaa aggcatctgt ccgccttcga agccgaccct tacaccgccg
    24061 ccggacctgg atttctgtcc tttttggcct ttgcctgcgg ttttgccctg acctgttgcc
    24121 gtacctctgc caagtctttt cttagacttc gttgtacctt ctgcaggttt aagctcgtgt
    24181 aatttcatat ttacacctcc ttgattacat ttcctttact tcaaccaaat gtataacctt
    24241 ctgaatcata cctcttatct gaggtgtatc gttatgttca acttcactgt tgattttttt
    24301 gagtcccaga gccttaactg tagctatctg gtcatctttc ctaccgattg tactctttat
    24361 gagtttgact ttaagtttag ccaaggtact tccctcctag cctattatct cttcaaccgc
    24421 tttgcctctc agctttgcta catcttcaac cgttttcaat gaagcaaggc cttgaatcgt
    24481 tgcatttacc atgtttcttg gattatttga tctcaaggat tttgttctta tatccttaat
    24541 gcctgccagc tcaagaaccg cacgagcagg gccccctgct ataactccgg taccttcttt
    24601 tgccggcatg agaagcactc tgcctgcgcc ggcaactcca ataacctcat gaggtattgt
    24661 agttccgact atggatacct ttataagatt cttcttggca tcttcgatac cctttcttat
    24721 tgcttcaggt atttctactg attttccggt gccgactccc acatagccat tcatgtcgcc
    24781 aaccactacg atggcgctga atctgaagtt cctgccgcct ttaacaacct tagcaactct
    24841 atttatggca acaacctttt ccttaaggtc aagtttactt gaatctattg gctgcatcta
    24901 tttccctccc ttatcctaga attccaatcc ggcttctcta gctgcgtcag ccagttcctt
    24961 gactctgcca tgatatatat atccgcctct gtcgaaaaca acctgcttaa tgcctgcatc
    25021 taatgctttt tttgctacca gcgtgccaac ggccttagct gattccttgt ttccgccgtg
    25081 acctggatta tcctttaact ctttatccag tgttgaggct gatactaaag taacaccctt
    25141 tgtgtcgtca attatctgtg catatatgtt gttaaggctt ctgtaaacat ttaatctggg
    25201 cctctctgat gttccgaaaa ccttctttct cattcttaaa tgtcttttct gccttgtact
    25261 gtttttactg gctttagtta tcaccctgtg tcaccccttt gctatttatt tcttacctgc
    25321 ttttccttct ttacgcctaa ttacttcatc agagtactta atgcctttgc cgagatacgg
    25381 ctccggctct cttacagctc tgatattggc agcatgtgtt ccgacctttt ccttgtccgc
    25441 gcccctcaca attattttgt tcggagccgg tacctctgtt gttatccctt ccggatcatc
    25501 catttcaaca ggatgtgagt aacccatcgt aagtgtgagc ttcttgcccg acttggcagc
    25561 tctgtatcct acgccgttta tttcgagtac tttctcaaat cccttcgaaa caccctcaac
    25621 catattgttc aaaagcgctc tggtaagtcc gtggagtgcc ctgtgcattt tctggtcggt
    25681 tggccttgtt actaataatt tgccgtcttt tttctctata ttaatatctt catgcatctt
    25741 ttttataagc tgacctttag gccccttgac tgaaaccgta ttgtcatcag ctatttttac
    25801 ctcaactccc tggggaatct caataggtaa tttccctata cgtgacatgc ttgcacctcc
    25861 tctatacgta agatattacc aaacgtagca aagcacttct ccgccgacgc cctgaagtct
    25921 cgcattcttg tccgtcatta tgcctttgga tgttgaaatg attgctatgc ctagccctcc
    25981 aagtaccttt ggtatatctt cattttttgc atatacttta agtcccggtt tgctaattct
    26041 ctttaatcct gttattacct tctgcttgtt tgatccatat ttgagactga ttctgataga
    26101 atttgtgctt ccgtctccga ttatttcata accctttata tatccttcat caagcaggat
    26161 ctgcgcaata gcttttttca tattggatgc aggaatatca acagtttcat gcttgactgt
    26221 gttggcattc ctgattcttg ttagcatatc agcaattgga tcagttgtaa ccattaatct
    26281 taacctccct ttttcagatg tcatctttct gaggtctgag accagaaatc ggaggactga
    26341 ttttccctcc tgttctgaac tccgccataa acttttattc taactttata tacaattctt
    26401 ccgcctgtct tgtctgcctt cgggcatccg gcttccgact tccgacttcc gaaattacca
    26461 gctggccttc tttacacctg gtatttgccc tttataagcc aattccctga agcagatacg
    26521 gcagataccg aacttcctca aataagcatg aggccttccg catatcttgc atctggtgta
    26581 agctctggtc ttgtactttg gtgttctctg ctgctttaat accattgatt ttttagccac
    26641 aattttacct ccttcgcaaa gtttctgtat tagcttgcat aaggcattcc aaggagtctc
    26701 aagagttctc ttgcttcctc atcagtctcg gctgtggtta caaaaattat atccatgcct
    26761 cttattttat caactttatc atattctatt tcagggaata tgagctgttc cttgattccg
    26821 agggagtaat tgcctcttcc gtcaaatgaa ttaacggata cgcccctgaa gtctctaact
    26881 ctcggcagag ctatattgaa taatttatca aggaattcat acatctgatt gccgcgaaga
    26941 gtaaccttag cgccaacaga cattccctgt ctcactttaa atgccgctac cgattttttt
    27001 gcttttgtta taaccggctt ctgtcctgtt attgttccaa gctcagatac tgcggagtca
    27061 aggcctttgg cattgtcttt agtatcaccc aagcccatat ttataacaac cttctcaagt
    27121 tttggtattt gcatcacact tttatattga aacttctgca tcattgcagg agctacttcg
    27181 ttcaggtatt tctcttttag tctggccatt tcttattacc tcccttcaga gactatttat
    27241 ccaatatttc gccgcagctt ttacagtatc tgacctttga cttgtcatcc agtattctgt
    27301 gtcccagcct tacgcctttt ttgcatttgt cgcaccaaag cataaccttg gaactgaata
    27361 tcggcgattc ctgatgtatt attccgccct gctgcatatt tttgccgggc tttttatgct
    27421 taacgctcat gtttacaccc tcaacaagta ccttgtcttc ctgcggtaaa gctataagca
    27481 ctttggcctt cttgccttta tccttgccgg agatcaccac caccgtatct ccctttttaa
    27541 catgtaattt tttaacttct gcatttgcca ttcaaaacac ctcccttaaa gcacttcagg
    27601 tgcgagggat attattttca taaaatcctt ttcacgaagc tccctggcta caggcccgaa
    27661 tatacgggtt cctcttggct gtttatcttc ttttatgatt actgctgcat tatcatcaaa
    27721 tcttatatag cttccgtccg gacgtctctt gccctttaca gatcttacga taacagcttt
    27781 tacaacttct ccctttttaa caacaccgcc tggtgttgca tctttaacag tagcaactat
    27841 tacatcgcct atgtttccat atcttctggt ggaaccgcct actacgcgaa tgcacagcag
    27901 ttccttcgcc ccggaattgt ccgctacgtt caaccgtgtt tcctgttgaa tcatttaaaa
    27961 ccctcctttc ggaattttac ttggccttct caattatttc aacaagtctc catctcttat
    28021 ctttgcttaa tggccttgtt tccataattt taactttatc gcctatcttg cattcgttgt
    28081 tttcatcatg ggccttgaac ttagttgttc tctttattga tttgccgtaa agaggatgac
    28141 gaaccaatgt ctcaatgcct acaactattg ttttgtccat cttgtcactt acaaccttgc
    28201 cgattcttgt cttcctatta tttctatcca atgtctaaac ctccttctta ctactttgcc
    28261 tgtacagact taagttcatt ttctttaagg atggttttta ttctagcaat agatttcttc
    28321 acatctttta ccctcatagg gttctcaagc tgtcctgtag ccatttgaaa tctaagatta
    28381 aaaagctctc cttttaattc attcaacttc tgttccagtt cttttaccga catatcgcga
    28441 aacttgttag ctttcatttg tatcaccaac catttcctca gtttcccggc gggttacaaa
    28501 cttgcacttt atgggaagct tattctgcgc cagcctaaga gcttctctgg ctatctcttc
    28561 ctcaacaccc gctacttcaa ataatactct gcccggcttt acaactgaca cccagtattc
    28621 aggagcaccc ttaccggaac ccatacgtgt ttcagcaggt ttctgagtga ccggtttatc
    28681 cgggaaaatc tttatccata tctttccgcc tctttttgta taccttgtca ttgcctgtct
    28741 ggcggcttca atctgattgt ttgtgatcca gcccggctca gtagcctgaa gcccgaattc
    28801 accatatgct ataaaattgc ctcttgttgc cttgccactc atgctgcctc tcatctgctt
    28861 acggtgtttg actctttttg gtatcaacat tatgcttctc ctccttccgc agctgcttca
    28921 ttcagcttag cctgctcgct cttacgagtt ttggcttgtg gaagaacctc ccccttatat
    28981 atccagacct taacgcctat ccttccatag gtggtttttg cttctgcgaa tccataatct
    29041 atatttgctc tcaaagtctg tagaggaatt gttccttcac gataccattc gcttcgtgca
    29101 atttctgctc cgccaagacg gcctgcgcac atagtcttaa tacccttggc ccctactctc
    29161 attgtccttt gcattgactg cttcattgcc cttctgaatg atattctctt ttccagctgt
    29221 gccgctatat tttctgcaac aagctgagca tccagttccg gtacttttat ctctgtaata
    29281 ttaactgtaa cacccttgtt gttagtgagc ttttccaatt gtttcttaag ctcttcaata
    29341 ccggctccgc cttttccgat taccataccc ggctttgcca cgtgtatatt aatctttatt
    29401 tttccggcag ttctttctat ttcaactctt gaaattcctg aggtgaaaag cttggttttc
    29461 aagaactctc ttatcttatt atcttctaca agaaaatctg caaagctctt atcatctgca
    29521 taccattttg tgtcccaatc cttaataatg ccaactctta gtccgtgcgg atttaccttt
    29581 tggcccacta aaatccctcc ttcttcactc tttttcctta accgccaatg taatatggct
    29641 tgttcttttt agtatcctta cacctctgcc gtgtgaagct gctctaaacc tcttaagagt
    29701 gggtccctga gtggcataaa tttctgagat atacaatttg tcaacattca tttcataatt
    29761 attctcagca tttgccaccg ctgattttaa gagcttctcc agtaacactg atgcggcttt
    29821 tggcgtatgt ttcaatattg caaaggcttc actgaccttt ttacctctta tcaaatctat
    29881 aactatatta acctttcttg aggaaattct aatatattta gctattgccc tagcttccac
    29941 aggaaatacc tccttccgta ttatcgctta gtttggcact ctttctattt taaagctgag
    30001 gacctctccg tatggtgtcc gtggcccctg aaagttctgg tgggagcaaa ttcacccagc
    30061 ttgtgtccta ccatatcctc tgtcatgtat acaggaacat gcttcctgcc gtcatataca
    30121 gctatggtat ggccgaccat ttcagggaat attgttgaac tccttgacca ggtcttaagc
    30181 acaacctttt tgttggtctt attcatatct ataattttct ttaatagctt ctcatcaaca
    30241 taaggtcctt tttttgttga gcggctcatt gatttgcctc ctttcgagta ctatttctca
    30301 tttcttctct taacaataaa cttgctcgaa gctttcttat tgtttctggt cttatatcca
    30361 agtgtcggct tgccccaagg ggtaacaggc cccggccttc ctattggtga cctgccttca
    30421 ccgcctccat gaggatggtc tgcaggattc attacagagc ctcttactgt cggtcttatg
    30481 cccatgtgcc tcttcctgcc ggcactgcct attgaaatat tttcatgatc taggttccct
    30541 acctggccta ttgttgcctt gcattcgatt ctaatcatcc tgacttctcc ggagggaagc
    30601 cttacctgtg catattcccc ttcttttgcc ataagctgtg cagagtttcc tgctgctctt
    30661 accagctgcg ctcccttgcc tgccttaagt tctatgttgt gtatcactgt acctatcggt
    30721 atgcttttta aaggtaaagc atttccgacc tttatgtcgg cattttctga cgatattatc
    30781 gtatctccca cttttagtcc gttaggagca ataatatacc tcttctcgcc gtcagcataa
    30841 taaagcagtg ctatattggc agacctgtta ggatcatact cgattgccgc aacctttgcc
    30901 ggtatatcaa ctttgtccct tttaaagtca attattctat actgtctctt atgtccgccc
    30961 ccctgatgcc ttactgttat cttgccgtaa ttatttcttc cagctttact ttttaatatt
    31021 tcaaccaggg atttctccgg ttcggttttt gtaatttctt caaaggttga aaccgtcata
    31081 ttacgtctag ctggagaagt cggattatac tttttaattg ccattttttc tcctcctttt
    31141 acagtccttc gaagaattcg atgcccttgc tatcttttgt aagctttatg attgctttct
    31201 tgtagccggg tcttcttcct tcatattttc ccattctctt cattttgccc aatactttga
    31261 ctgtgttgac tttgtctact tttaccttga atatctcttc aacagcattt tttatttcag
    31321 tcttgttagc attaacatca actataaagg tgtatttctt gtctgccatt tcgctcatgc
    31381 ttttttcggt tattacaggt tttctaacta tatcgtaagc attcattatg catacacctc
    31441 ctcgactttt ttaacagcat cctttgttat tacgaacttg tcatacttca aaatgtcata
    31501 tacattcaat gtatttacaa aagtggcctt tacaccgtct atgttccttg ctgatttaac
    31561 aacgttttca ttgttgtcag ccaaaacaat gagagtcttg gaatcaatct tgagattctc
    31621 aagcaagctt acaacctgct ttgtctttgg tgcatcaaag ttcagctctt caataacaac
    31681 tatgctattc tcattagcct ttgaacttaa tgcagacttc attgctattc tctttatctt
    31741 cttaggtact gtatacctgt atgatcttgg ctttggagca aatactatgc cacccttctt
    31801 ccactgcggg gacctaatac tcccgtgcct tgccctgcct gttccctttt gtctccaggg
    31861 ctttattcct ccgcctctta cctcagctct ggttaaggct gactgagttc cttgcctctg
    31921 gtttgcaaga tacatcttaa ctacctgctg cattgcttca acgtttacat ttacgccgaa
    31981 tatattttca tttagttcga tttctccaac ctgtttgcca cttatattgt aaagtgctac
    32041 attaggcatt ttacatcctc ctttctacct cgaatgcttt atcaagcttt cactgtatcc
    32101 tttatcatta ccagtgaacc tcttattccc ggaataccgc cttttacaag gataatgttc
    32161 ttttccgggt caacctttac tacttcgagg tttaccactg ttgtattttc tgcgcccata
    32221 tgccccggaa gcttcttccc gggaattgtt cttgagggac tggatgacgc acccattgaa
    32281 cctgcccatc tgtggtattt ggagccgtgc ttcattggcc ctctgtggaa attgtgcctc
    32341 ttgatggcgc ctgcaaatcc tttacccttt gatacgccgc taatatctac tctgtcgcct
    32401 tccttaaaga tgtccacctt tatttcctgg ccgacttcgt aagatgctgc atcttcaagt
    32461 ctgaattccc taagatatct ttttgttgca acctttgctt tggcaaaatg tccttttaat
    32521 ggctttgtga cctttttctc ggctttatca tcaaagccaa cctgtattgc ttcatatcct
    32581 tcattttcta cagttttttt ctgtacaact atgttgggat tcgcagctat tacagttacg
    32641 ggaaccgctt tgccttcatt atcaaatatc tgagtcattc caacttttaa acccagtatt
    32701 gcttttttca ttaattacac ctccgaaatt tacagcggac cgcttgccgg tcatactaaa
    32761 tttgatctgg ctcgtggttc agggtccgtg gttcggggtt tttgtccggc ttcgaagccc
    32821 ttctcaatcc tcgcacccgg tacctcgtac ctcgcacctg ttttataact ttatttcgat
    32881 gtcgacacct gccggcagat caagtctcat aagcgaatca accgtctttg gtgtcgggtt
    32941 cagtatgtca atcagtctct tatgagtcct catttcgaac tgctctcttg aatccttgta
    33001 cttgtgcact gcacgaagta ttgtgactat ttccttctca gtcggaagcg gcaccggacc
    33061 tgataccttt gcacccgtcc tttttgctgt ctcgactatt ctttctgctg actggtcaat
    33121 cagtatgtga tcatatgcct tcagtctgat tctgattttt tgttttgcca ttttcaattc
    33181 cctccttttc atcgcacgcc ttattttgct ggcgtgcaac cgcatactgt tatacactta
    33241 tccgggtgtg ttctattttt ttatcagtgt ccggcatccc ggacacttca ttttcacccg
    33301 cagcagcata atcagtagag tcgcccgaat tctagaaacg gacattactc tgtaagaatt
    33361 cagccgcaaa tcacttcaac tgcggctttc cagaatacgc ccattaaggc ttaatctgca
    33421 acctcttact tcatcgcatg ctgaataagt atcgcaacct tatatatttt ataacatttt
    33481 aggacaaatt acaatacctt taaaaccatt gtttaaatat ttttttactg tccattttta
    33541 cagcctcaaa ggaccgggat aatcccagtc cttgaagcct ttccaagtgc tcgtgcctcg
    33601 cacctggcaa cctcgtacct gcgaataggc gcagcctatt cgattaccga tgcaacagca
    33661 cctgatccga ctgttcttcc gccttcacgt atagcgaatc tcaagccttc ttctattgct
    33721 atcggagtga taagctttat tgtcatttct atatggtcac cgggcatgca catttctact
    33781 cctgccggta attcaattac tcccgttacg tctgttgtcc tgaaataaaa ctgcggcctg
    33841 tatccgttga agaatggagt atgccttccg ccttcttcct ttgtcagtac gtatacctgt
    33901 gctttgtagt gtgtatgagg ctttattgat ccgggctttg caagtacctg ccctctttct
    33961 atctcattcc tctgtactcc tcttaacagt actcctatgt tgtctcctgc ctgtgcctgc
    34021 tcaagaagct tcctgaacat ttccactcct gttactaccg tggatctcgg cgcatctgca
    34081 agccctacta tttctaccgt atccgatacc tttactgttc ctctttctac tcttcctgtc
    34141 gccactgttc ctcttcccgt tatcgagaat acgtcttcca caggcataag gaacggcttg
    34201 tctgtctgtc tctgcggtgt gggtatatag gtatctactg catccataag ctcatgtacg
    34261 ctcttgcacc attcacagtc ttcttttccg catccgcact ccagtgcctt taatgccgat
    34321 cccgctataa tcggggtatt atctccatcg aattcatact ctgtaagcag ttcccttact
    34381 tccatttcta caagttcgat aagttctgcg tcatctacca tgtcaacctt gttcagaaat
    34441 accactatgt agggtactcc tacctgacgg gacaacagta tgtgctctct tgtctgaggc
    34501 atcggaccgt ctgccgccga tacgacaagg attgctccgt ccatctgtgc tgctcctgtt
    34561 atcatgttct ttacatagtc agcgtggccc gggcagtcaa catgggcata atgcctgttg
    34621 gctgtctcat actcaacatg ggctgtgttt attgttattc ctctttcctt ttcttccggt
    34681 gccttgtcta tttcgtcata ctttgttgca gttgcctgac catactttga aagcacaact
    34741 gttatagctg ccgtaagtgt agtcttgcca tggtcaacat gacctattgt ccctatgttt
    34801 acatggggtt tggtcctttc atattttgcc tttgccattt tacttttcct cctttaaaat
    34861 cggataattc aatatcttag gaaattgtac aatatcaaat tgccatacga tttccctgtt
    34921 tttaatcact tttcacctaa tatcttctcc gctatgctct taggaacctc ttcaaagtga
    34981 tctgtctcca ttgagtatac tgcacgtcct tgtgtccttg aacgcaggtc ggtagcataa
    35041 ccgaacatct cggaaagagg tacaaagcct cttataacct gagccccgct tctcgcttcc
    35101 atgccttcta ttttccctct tctggagttt atgtctccga gaacatctcc catatattcc
    35161 tcaggcactg ttacttcaac tttcattata ggctccagaa gaacaggatc ggcctttttc
    35221 attccatcct taaaagccat tgaaccggca attttaaatg ccatttcgga agagtcaacc
    35281 tcatggtatg aaccgtcata cagcgtgact ttaacatcaa gtacaggata tcctccgagt
    35341 acgccgttca tggtggcctc ttccacaccg cgctgtacag cgggtatata ctctttcgga
    35401 atcacaccgc ctactatctt gtttacaaat tcaaaaccgg ttccgggttc cttaggctca
    35461 atctcaagcc aaacatggcc gtattgtcct ttgccccccg actgtctgac aaactttcct
    35521 tccacctgta ctttttttcg gatggtttct ttataagcaa cctgcggctt gccgacattt
    35581 gcttcaacct tgtactcccg catcatcctg tcaacgatta tgtccagatg cagctcaccc
    35641 atgcccgcaa ttatggtctg gccggtctca gcatcagtat atgtcctgaa tgtcgggtct
    35701 tcttccgcca gtttctgcag ggctataccc atcttatcct ggcctgcctt tgttttgggc
    35761 tctatggcga cattgataac cggttccgga aaatccatcg attccagtat aactgcattt
    35821 tcctcatcgc aaagggtgtc tcccgtagtc gtatccttaa gccccacaat tgctgcgata
    35881 tcgcctgtat aaacctcttt tatctcttcc ctgtggttgg catgcagctg cactattctt
    35941 ccgattcttt ctcttttgcc ctttgttgaa ttatatacat aggatccggc ttcaaccatc
    36001 cccgagtata ctctgaagaa ggcaagctta cccacataag agtcgaccat tattttgaat
    36061 gcaagagctg agaagggttc cttgtcatca gaatgcctga caatcttctc ttctccatct
    36121 acagtagttc cttcaatggg cggaatatca ataggagaag gcataaaatc cacaacagca
    36181 tccagcataa gctgtacacc tttattcctg tacgaggagc cgcaaacaac cggattaatc
    36241 ttgacactga ttgtaccggc cctaatggca tccttgatct cctgctccgt cggttcttct
    36301 ccctccagat acttcatcat gagatcctca ttcatttcgg atacagcttc aattagcttc
    36361 tccctgtatt cttttgcctt atccatcatg tctgaaggta tttcccctac ctccatattt
    36421 ttgccaaggt cgtccttgta aatatgagct ttcataccta tcaagtccac tatacctaca
    36481 aatctgtctt cgctgcctat cggaagctgt ataggaacgg cattggctcc gagcctttct
    36541 ttcatcattt cgactactct gtagaaatcc gctcccataa tgtccatttt attaatatat
    36601 gcaatccgcg gaacattgta tttgtctgcc tgcctccaga cggtttccga ctggggctca
    36661 actccgccct tggcgcaaaa aactgctaca gctccatcca atacgcgcag tgacctttct
    36721 acttcaacag taaagtccac gtgccccggc gtatctatta tgttaatctt atgatccttc
    36781 cactcagccg ttgtagctgc agaagttatt gttattcctc tttcctgctc ctgctccatc
    36841 cagtccattg tggcagttcc ttcgtgaact tcacctaatt tataggtttt tcctgtataa
    36901 tacagtactc tttcagtcgt tgtcgtcttt cctgcatcaa tatgggccat tattcctatg
    36961 tttctggtct tttccaacga aaattgccta ggcacaatta tcctcctttc tgttgcaagt
    37021 atcactgtta tacttcccga aaattttaat atttagcatt aatttataat catttttata
    37081 agtcacaatt acctctttta agattaccac ctgtaatgtg caaaggcctt attggcttct
    37141 gccatcttat gagtgtcttc tctcttcttc acgctgctgc cggtattgtt ggcagcatct
    37201 aaaagttcac cggccaatct ttctttcatg gtcttttccc ctctttttcg ggcatactcg
    37261 atcagccacc tcatccctaa tgcttgcctt ctgtcaggcc ttacttctat aggaacctgg
    37321 taagttgctc cgcctattct tctggctttg acttccagaa caggcatgat attgttcata
    37381 gctgtctcaa atacttcaag ggggtcttta cctgtctttt ccctcagtat ttcgaaggca
    37441 ccataaacta ttctctgtcc gacacttttc ttgccgtcat acataacgtt gtttataagc
    37501 ttggtaacta tcttatcgtt gtataacgga tccggcaata cgtccctttt tgatatatgt
    37561 cctcttcttg gcactatact tccctcctta atattatttt aatatcatcg gtactcggca
    37621 gtatcaagcc aaccaactgc cgttgtgcgc aaatatattc aaaacaaagg ctgcagtcag
    37681 ccaatgtcaa ataactcgca caatattatt tctttggtct cttggcaccg tactttgatc
    37741 tgctctgctt tctgtcagcg acgccagcgg catcaagagt acctcttact atgtggtacc
    37801 ttacgccggg aaggtcctta actctgccgc ccctgataag aacaaccgag tgttcctgca
    37861 ggttatggcc gattccgggg atgtagcctg atacttcaat gttgtttgaa agtctaaccc
    37921 tggcaatttt tctcaatgcg gaattaggct tcttgggtgt tgtagtcttt actacagtac
    37981 atactcccct tttctgcgga ctttccttca atgctggtgc agttgatttg tactctacct
    38041 tctctctgcc tttcctcact aactggctaa ttgtaggcat taaatgcacc tccttgtttt
    38101 atttatcata tacaggcccg gtatttgcca agctctgcta tacgcggtga aaaccaatac
    38161 actaaaaaac ctcttcatat gaagaggtct gatatgcata ctaatccttg atagccgccg
    38221 tcgaggcgcc gatatcaatg ccgcatgcat ctccaagctc tttcatattg ctgacatata
    38281 tgatttgtat atctctatct ctacatacat ccagtaccga ttgcttaata aagtcatcgg
    38341 catcctctgc caggtaaacc ttttttaccg tcccgctttt tattgccttt actgtctgct
    38401 tcaaccccac tgttcttctg tgtatggcct tcagttcctg cattcaaacc acctcttaag
    38461 gatttttttg catggcagca tcagacatca acaatcaaat tataaatact gtattcaact
    38521 gcaagcttag aaaaccatta ttaatggttt tctataaaac actagattag tttagcattt
    38581 gttataacat gatgtcaata atggtttttt gcatttgcgc aaaaaacttt tcccgcctca
    38641 tattactcgg cttccacctg ttttattgat atatccatat attttttcat accggtgcct
    38701 gcaggtataa gctttccgat tattacattt tctttcaggc ccacgagggg atctatctta
    38761 ccctttattg cagcctcggt aagaaccctt gttgtttcct ggaatgatgc ggctgacaag
    38821 aaggaatcgg ttgccaggga ggccttggtt ataccaagga gtactctctt ccccgtagcc
    38881 atctctccgc cttcggcact taccctttgg ttttcttctt catatttaaa tatgtcaaag
    38941 agccctcccg gaagcagatc ggtatctccg gattcttcta tcttgacttt tctcagcatt
    39001 tgtctgacaa tgacttctat atgcttatcc gcaatatcaa caccctgaag cctgtatacc
    39061 ctctgaactt cctgaagcag ataggattgc accccggcta ctcctttgat tttcaatata
    39121 tcatgaggat taaccgaacc ttcggttatt tcatctcctg cttcaattac atctccgtcg
    39181 gcaaccctaa gccttgagcc gtatggtatt gtatatgtcc tggaatcgcc gtcttcagcc
    39241 agcacgacaa catctttctt tttcctctgg tctattattt ttacaacacc ggagatttcg
    39301 gatattattg caagcccctt aggcttcctg gcttcaaaaa gctcttcgac tctgggaaga
    39361 ccctgggtta tatcatcacc ggcaacgcct cccgtatgga aggttctcat tgtaagctgc
    39421 gtaccgggtt ccccgattga ctgagccgca attattccca ctgcctcgcc gatatgcacg
    39481 ggatctcctg ttgccaggtt taccccatag catttggtgc atactccata ttttgaatga
    39541 catgtcagta cagaccttat cggaactttc ttaatcccca gcttgacaat actatttgcc
    39601 ttcttttcgg ttataagctc attcctctta accagtacct cccctgtctc aggatgcagt
    39661 atatcgctgc tggcgtatct gcccataagc ctgtctttca gcttttcaat tacttcattt
    39721 ccgtctttga tttcgctgac catcaaaccg tcttccgtac cgcagtcctc ttctctggct
    39781 attacatcct ggcttacgtc aacaaggcgt cttgtcagat agccggagtc ggcagtacgc
    39841 agcgcagtat ctgccaagcc ttttcttgca ccgtgggttg acgtgaagaa ttccagtaca
    39901 tcaaggcctt cacggaaatt ggcctttata ggaatctcta tgattctgcc tgaggtatcc
    39961 gccatcaggc ctctcatgcc gcacaactgc ttgatctggt ttacgctgcc ccttgcgcct
    40021 gagttagcca tcatatatac gggattaagc ctctccaggt tatccatcaa tgcttttgtt
    40081 acatcttcag ttgctatttt ccaggtctct attacctgct catatctttc atcgttggat
    40141 ataaggcctc ttctgtacat tttttctatt ttgtctactc tctcatctgc ttttgaaagc
    40201 agctccttct tggccatcgg tatatccata tcagatatgc ttattgttat tgcacccttt
    40261 gttgagtaat gaaagcccag tctcttaatt tcgtccagca caattgcggt atttgtgttc
    40321 ccatgcattc tgtagcattt atccactatt ttacccagga gcgacttatc cacgggcatc
    40381 aaatttccgt ccttatccct tggatcaagc tccagaagga acatatcctc ttcggtattt
    40441 ctctcaacaa aaccaaggtt ctgaggaatt gactgattga atatgaatct tccgacagta
    40501 ttttcaacaa acctgctgac cattctcccg ttaatctctt tggttattct taccttaacc
    40561 tttgcgtgaa gggaaacttc atccaattga taagccatga gcatttcgtt caaatctgta
    40621 aattccttgc cttcgccctt atgccccggc acataaatgg taaggtagta tacccccata
    40681 accatatcct gagtcgggct gacaaccggc cttccatcct tgggagccag tatattattg
    40741 gcggacagca tcagaaatct tgcctctgcc tgtgcttcaa ccgataatgg tacgtgcacc
    40801 gccatctggt ctccgtcaaa gtcagcgtta tatgcagtac ataccagggg atgtatcttc
    40861 aatgccctgc cttgtacaag cacaggttca aaagcctgta tccccagcct gtgaagtgtt
    40921 ggagcacggt ttaaaagcac cggatgctcg cttattacct cttcgagtat gtcccagacc
    40981 tccggtttca ctctttcaac cattctcttg gcgcttttga tattatgagc aagcccgtca
    41041 ttaacaagct tcttcattac aaaaggttta aacagctcca aggccatctc attaggtaaa
    41101 ccgcattgga aaagcttcag ctcaggccct acaactataa ctgaacggcc tgagtagtcc
    41161 acacgttttc cgaggaggtt ttgcctgaac ctgccctgct ttcctttaag catatcactt
    41221 aaggatttaa gaggcctgtt gccggggccg gttacgggcc ttcctctcct gccattgtcg
    41281 ataagtgcgt caactgcttc ctggagcatt ctcttttcat ttctgactat gatatcgggt
    41341 gctcccaagt ctaacagcct cttaagccta ttgttcctgt ttattactct tctataaaga
    41401 tcattcaggt cggaggtggc aaatcttccg ccatccagct gcaccatcgg cctcaaatcc
    41461 ggcggtatta caggtatcgc ctcaagaatc atccactcag gcttgtttcc ggaattcttg
    41521 aaagcctcga caacctccag ccttctgact gttctaagcc ttttctgccc tgtactgcct
    41581 ttaaggtctt gcctcagttc ctttgacagt ttttcaaggt ccaaatccag aagcagttct
    41641 cttattgatt cagctcccat tcctgccctg aatctgttgc cgaatttctc aatattttct
    41701 ctgtattcct tttcacttag cagctgcttt tttgaaagag gagtatcacc gctgtcaatt
    41761 ataatgtatg aagcaaagta cagaaccttt tccaaagccc tcggggacat atcaagtata
    41821 agccccattc tgctgggtat ccctttaaaa taccaaatat gggatacggg agcagcaagc
    41881 tctatatgcc ccatcctttc tcttcttacc ttagccttgg ttatttcaac accgcagcgg
    41941 tcgcatacta ttcctttgta cctgattctc ttgtatttcc cgcagtggca ttcccaatcc
    42001 tttgtaggac cgaaaatctt ctcgcaaaaa agtccttcct tctcaggctt cagtgtcctg
    42061 tagtttattg tctcaggctt ctttacctct cctcttgacc actctcttat tttctccggg
    42121 gatgccaaac caatttttat tgcttcaaag ttatttaatt caaacaagga gttatctctc
    42181 ccttctaaaa attttagaac tcttcgccat cctcttcaac ttcttcttca acttcttctt
    42241 caacttcatt atcacctatg agaaggtcat ccaggtctat ctctgcatca aagtcttccg
    42301 ctccttcgga ttccatctca taatcctcgt aaagatcctc cggagtgacc gtctcccctt
    42361 tatatgaaag actcgagaaa tccatgtctt cccttacttc catatcagcg cccaaatcct
    42421 ccacatcttc ttccaccgat tctcttatgg taatctcttc atgctcctcg gtcagaacct
    42481 tgacatcaag ggccaggctc tgcagttctt ttataagaac cttgaaagat tcaggcacgc
    42541 cgggctcggg tatgttttcg cccttgacaa cggattcata ggtctttact cttcctacta
    42601 catcgtcaga cttaacagta agtatttcct ggagtgtatg ggctgaacca taggcttcca
    42661 gtgcccacac ttccatttca ccgaacctct gaccgccgaa ctgggcttta cccccaagag
    42721 gctgctgcgt tacaagagag tatgggcctg tggaccttgc atgaatcttg tcatctacaa
    42781 gatgcagcag cttcagtata tacatatagc ccactgtaat atcattgtcg aagtactctc
    42841 cggtacgccc gtccctcagt gtagcttttc ctgtatgatt gagttcaatt ccgtcaatag
    42901 gagtttcacc aaatgccttg agtattttct caacggactt taattccgta agatctgaag
    42961 actttatcat ttcccttaag gaggtgaact tgctgtcaat gtcatagatt tcatgattta
    43021 acagttcgta aattatttca acattttctt ttatttcaga cctgatatcc gtttcagcaa
    43081 gctgctgccc ctcataatct tcatcattct gcttgtcggc aataagaatt ctctctttta
    43141 ttattctcag gtttttcttt acctgaagca ccttattggc gcattcgatt gtctctaaga
    43201 tactttcctc cgttgccccg tcgaataccg gagtagcaat tttccagccc agtgctttag
    43261 ccgcaagtgc aagatgcacc tcaaggacct ggccgatatt catacgtgaa ggcacaccca
    43321 gaggattcaa tactatctca agaggtgtcc cgttgggcat aaagggcata tcctcctccg
    43381 gaagcactct cgatataaca cccttgttcc cgtggcgccc tgccatcttg tcaccaacgg
    43441 atatcttcct cttttgtgct atataacatc ttaccagttc gttcactccc ggaggaagtt
    43501 catctccgtt ttctctggta aacactttta cgtctacgat tatccctgat tccccgtgag
    43561 gaactctgag cgaagtatct cttacctccc ttgccttctc accgaatatc gcccttaaga
    43621 gcctttcctc ggcagtaagc tcggtttctc ctttcggagt cactttacct accattatat
    43681 ctccggctct tacttcggcg cctattctta tgatgcccct ttcatccaga tccttcaagg
    43741 cttcttcccc gacattcggt atgtcccttg ttatttcctc ggggccaagc tttgtatccc
    43801 ttgcttccgc ctcgtattcc tctatgtgta tggaggtgaa ggtatcctct tttacaagct
    43861 tttcactgat aagtatagca tcctcgtaat tgtagccctc ccaagtcatg aaaccaatga
    43921 gcacatttct tcccaatgct atttcaccca tatcggtaga aggcccgtca gctattatgt
    43981 cgcctttttt cagcacttcg ccctttcgtg ctacaggcct ctgattgata caggttccct
    44041 gattggagcg caggaacttg agaagcttgt atcgctcctt ttcacctaag ctgttccgca
    44101 taactatttc attggcgctg accttttcaa ccacgcaatc gctgtttgcc aatcctatga
    44161 cacccgaatc cctggcagat ttgtattcca tgcctgtacc tactataggc gaatcgggct
    44221 ttattatagg aactgcctga cgctgcatgt tcgaacccat caaggctcta ttggcatcat
    44281 cattttccag aaagggtatc atagcagttg caacggatac tacctgcttt ggggatacgt
    44341 ccatatattc aatctccata gtaggtacaa taactatttc attggcatcc cttgccgtaa
    44401 cccttctgtt aacgaatctt ccttcttcat ccagaggttc gtttgcctgt gcaattacat
    44461 actcgtcctc aacgtcggca gtaagatata caatttcatc agttacatat ttttcaccat
    44521 tatcatcagt cttgacagtt ctgtacggtg tttcgataaa tccgtactca tctaccttgg
    44581 cataagtact caaagaaccg atcaggccta tattaggacc ttcaggggtc tctatggggc
    44641 acattctgcc gtagtgggag tggtgaacgt ctctgacttc aaaccctgct ctttctcttg
    44701 acaaacctcc gggtccgaga gctgacagtc ttctcttatg cgtaagctca gcaaggggat
    44761 ttgtctggtc catgaactgt gataactggg aacttccaaa aaattccttg attgctgcgg
    44821 ctacaggcct tatattaacc agctgctgcg gtgtgacaac atcaatatcc tggatagtca
    44881 ttctttcctt tacaactctc tccatcctgg ataagcctat tctgaactgg ttctgcagca
    44941 gctctcccac cgacctgagt cttctgttgc ccagatggtc tatatcgtct ttgttcccga
    45001 taccgttaga aaggtttata ttataattaa tagaagcgac tatatcatca atcagaatat
    45061 gcttcggtat aagctcctta atccggttcc tgatctcttc cttgatttcg ctctcagaac
    45121 ctgccttttg cagtatatcc ttcagcactg ataaattgac caattccttt atattaagat
    45181 ctgatacatc aaagctgagg tatttgttta tatccacagt attgttcccc agtatcttaa
    45241 ccgtctttcc ctcggctgtg acatatactt cgtttatacc tgcattctga attttcacag
    45301 ctttatccct ggtgattttc tccccttctt ccgccagaat ctctcctgtc ctgggatccg
    45361 ccaactctct gtccagcttt tgcccggtca gcctgctgct caggcttagc tttttattga
    45421 atttatatct tccgaatttg gcaaggtcat atctttttgc atcaaaaaac aaggcttcaa
    45481 gcagggattt tgcgctttct acagtcggtg gctctcccgg cctcaggcgc ttatacactt
    45541 ctattaagcc ttcttcctca ttctttgtcc catccttttg cagggttgct tttatcctat
    45601 catcttcccc caatattcca tcaatctgtt catcggtgcc aaagcctaaa gctctcaaaa
    45661 aaacagttat agggattttt cgggttctgt cgattcttac ccaaaggata tcattggaat
    45721 ccgtctcata ttccagccat gcacctctat taggtataac agtgcttgaa tacagcttct
    45781 tcccggactt gtcgatggtc tctgcataat aaacccccgg tgaccttacc agctgactta
    45841 ctataaccct ttctgcgcca ttgataataa aagtacccgt gtctgtcatc aaaggaaaat
    45901 cgcccatgaa aacttcctgt tccttgacct cattggtttc cttattgatg agtctgatct
    45961 taacttttaa aggagcagag tatgtaacgt ccctttcctt gcattcctca actgaatact
    46021 tcggttttcc gtctaacgaa taatcaacaa attccagaat cagattacct gtgtaatcct
    46081 gaataggtga aacatcattg aaaacctctc taagaccctc ctccagaaac cagttatagg
    46141 agtttttctg cacttcgatc aggttaggca tatccattac ttcgtcgatt tttgagtagc
    46201 ttatcctctg atttcttccg gcagagattg gatgtaccag cataaagtcc ttcacccctt
    46261 aaatttaaat aaattaaaat acccaaaagc aaaaaaattc ccaaaaagta acgcttttgg
    46321 tttgtccgta cagttaaagc acacctattt taatattgcc ttttttaatt atacttattc
    46381 aaatgcagct tcaaatgcag cagtatagcg caaaaaaact tactacaatg caatatataa
    46441 tgttaacata ccaataaatg actgtcaagc ataaactgat aaaattggta tgtattttgc
    46501 taatttttct attttctgat ttgtcaatag gtatttttat ttcgacagcg aatacaatgc
    46561 acccagaata gcagcagtat atgaagctgc cgacaatagt gctattgaag ctatctcaag
    46621 cttgaaaact gctttctgcc ttgctttgtt tactgccagc atatttttca ccgattcaag
    46681 ctccttctgt atatcctcta ctctctgata cctgtcctca ggcttgttct ccatgcattt
    46741 ggaaattatc ctgccgacat tagatgctgc cctgctcttc ctgcgcctca gactgtaatc
    46801 ggcacttccg ggaagcctgc catacagcaa atgatatatg gttaccccca tgttatatat
    46861 atcggttctc tgttctgtct ttcccccatt tatcaactgc tccggtgccg cgtatgcact
    46921 tgtacctgca aagaaggtat ctccggattc atactcgcag tactcctgag atatcccaaa
    46981 gtcaaccagc accagtctga ccccctgtgc aagtataata tttgacggct tcatatccct
    47041 atatataata ggcctcggct tcatgccgtg caggtatttc agtatatcac acagctccag
    47101 aaaccagtct gttactgtct cgagatcaaa actgccgtgc cttttaagca gtttgtccaa
    47161 ggaggaaccc tctatattgc tttctacaat ataaataccc tgctcattgc ggaatgcgtc
    47221 aacaaccctt ggcaggcttg ggtggtataa ccttttcagt atctcaatct ccgacaaggt
    47281 tttatcattt atcttgtccg gcggaatatg ctttacagcc catttgtttc cgatctcaat
    47341 attggtgcat aaatagacag agcctgtccc tcctttaccc agaagccttt ctattcggta
    47401 ctttccattt aacacgtctc cgattatcat ctttcaccct ccattgcaca gtgccgtcat
    47461 tccagtggca gcgtatcatc acagggggct gctgcagtag ttgcatcttc aataccttcc
    47521 gccccggtca gcctgaaggt tatcacttct tcacagtccg ggttctttcg gcagctctta
    47581 tttctatgtc ctctgattat gtgcagggta agtgcaaggt ttagaataag agtaaaaact
    47641 ccgattgata tgtaaatcat atgaagtttc ataaggttac ctccgacatg tcctgatttt
    47701 acagttcttc ctgcatcttg aaaacatatt cgtaatttga aaactttatt ctatcattat
    47761 cctttattcc gtattccctg ttgctgggaa tttttacgcc atttacaaaa gttccatttt
    47821 ttgagtttag atctcttatg taataaaacc cttcgttaaa ggtaatttct gcatgaagct
    47881 ttcctattgt acctccctgt atgatatggt ctgccatact gcctaatctt cctattataa
    47941 aaccatcctt gtttataatc accctttcaa ccctattggg ccctatgctt tcaagataag
    48001 gacggcaatc cttaggaatt tcagaaatgc aaacggtgtc acaggctttt actacatccg
    48061 gcgatgaaaa caccggttta tacctgctcc taaggacttc atcatggcgg cctgcacttc
    48121 tttttacttt atcttgtgct atacgctgag tatcatctgt tgccgctctc ttaccccggc
    48181 tgcccgatat cctattaatt acgagaatat ccagtgcagc tgaaataatc accacccctg
    48241 caatggatac aatatcgccc attttccggg atatcaaaaa taggcatgcg gcagctgcag
    48301 ctactaaaaa aatcagctgc agcagcatga tgcttcggat attgctgctt tttcccgcat
    48361 cttcatatgc cctttccttc gggcttctgc tgctgggggt gcttatgccg gcagctgctt
    48421 cttgaacggc tgccccctgt ccatattcat ataattctcc gctgctcctc aaatccacta
    48481 tcaacctgtt aaaatcaatg agactgaatg tatctgattt taaataattc agtattctct
    48541 gcagataatt atcccttgca ttatcatctg cattggatga gttaaccaca atatccttaa
    48601 aaaaggatct taatatctcc gttacattcc tattgcagga aacaggtaca tataacagcg
    48661 aagcttccgc cgtcgcagga ttaataaaaa tataatccgg atggatgatg aatccggaaa
    48721 gatccaataa gtaattaccg ctaagcatca ggttttttgc aatgcttctg agcagatcca
    48781 aaagccccgc cctgttaagg ctttttcttt cgatgtaatg ggataatgat attttggagg
    48841 ttatattata atagataaag gtgttttcat tttcccgcct tacatggaat ggcataaaag
    48901 cagaattcgg attctgacat agtatctcta cctgatgctt caacagtgca gattcactgt
    48961 caatcttaag aaccaaataa ctgccggcag cattattatc atagctgtat tcaaaatcca
    49021 atactgtatt tctcacatca actacctccc ggtttaatta aggtattatt actgccagca
    49081 ctcctgccag tataaagggc gcaaaaggta tttcgctttt cttgttgtca ggattcaggc
    49141 atattaatac tatgctgaac aagccgctta gaattgcaga aatcagcatt gcggatatta
    49201 ttctttcaaa gcccaggaat attcctgaac agccaaagag ttttacatct ccgagtccaa
    49261 ggccgccctt agtaatataa tggataagaa gcagcaccaa tcctgctgtc acaccgccca
    49321 aaatgctgtt taaaaattct ctttgggggt caatcaacac aaaggccatt ccggctattg
    49381 tacctgcaag caccagctta tccggtatcc tcttctttct gatatcaata attgctgctg
    49441 cagcaagtaa tatgataatt gttaaatatg gaagataatg actcataata ccctcctaat
    49501 gagtatttgc cctatcccag cggtttgcat accttgcacg gagtatagcc cgatccttcg
    49561 gcttcttcaa ttaatattgg aatcctgctt tttctaagat atcggcagtc cccttcatgg
    49621 aagcggatac ctgtccttgt aatatatacg atactgcctt ccggctccag gtccaaacct
    49681 tcatcctcgg ctgtcagtat tccgaagctg ccgttgatcc acgccctttc cacagctgta
    49741 tgaatcattc ttatattgaa aggctcaata aaaggaagtc tcaccaaata ttcgtactcc
    49801 aactgaagca caacgtcatc cctgctgaaa tccttatccg gagtcaaccc aagttcacta
    49861 tatattgctc tttgtgacct tgcgttatat tcttcctcgc cctgaggaaa ctcaacaagc
    49921 cgcagcctta tattctcccg attaatcagc gggcttttca ggtgttcctt gataaacgct
    49981 tcggcaatat aatatttggc atcagacttc atgtcccaat atgcaggtgt tattctaccg
    50041 acaacatttc ctattgcgcc tttaccgtaa tcctttaata tgtcctgcac cgcttcagtg
    50101 aggtctgttc cttcaagctc ccccgataca atagctcgaa acaggttatc agagctgcct
    50161 cccgaaattt ccccagccag cttttccagt tcctcttcaa gagtaggtat ttttgcattc
    50221 ccatactctc gatgcttttc atcctccagt tcattaatga aggatatggg atatgcggct
    50281 gcagctattt cctttgctgc ttcatttaca acatgatcaa gcaccatccc ggtacatgta
    50341 aacttgataa gaaaaagcag caggaaaaaa acgcttatga aaaccggaaa tacagcacaa
    50401 gcctctactg ttattgaacc tctggaacgt agttttttat aacctttata caatttcaac
    50461 caccctaata gcctacatat atttgcttat atattaaata ctttccgttt tcaaaactgc
    50521 cgggcagcag cctgtcaacc ttcagcaggg gaagaaaaaa aagatttacc ttcgccttaa
    50581 cagaggcaga tatgacggtg ctgtaatctc ccagcctgaa atccgacctt ggaagctctg
    50641 tctttgcatc cacaacctgc ttaatgttta cttgaataag ttgtcttgcc cttcttaaaa
    50701 tttcttcctc cggcttcatt agaagcagta tctttagatg atccgaatac tttaatctaa
    50761 ctgttgtaaa gctcttcggg catattggca cagcctcgcc gttaagcata ttgaaaatat
    50821 ccaaacttga gtcaagggca ccttcggcaa gggcaaatgc cagcctttga ggcggaaata
    50881 ccacagtgct ccttataaaa gcatctattg tatctaatgc aaatctcaat gcccaaacct
    50941 gaagcagcac ttttgttact acataatact cggtattttt tatcggactg tatccttcct
    51001 caatatctga tccgctgatg atatactcca cttcactctt tctgaagtaa tgaccccttt
    51061 ctgtcccgga ggtcaggaag gtatacttgt ccattacgta ttcaatggta tttacctttt
    51121 caaatgcgtt tgatgcgaca tctccaattg cttttgcaag cttttccaag gttttgatta
    51181 tagcatcatt gcttttctcg gcttcttctt cccgcattga tgcgctgaaa tatgtacttt
    51241 cgtccatttc gccaaagttc tcgccggtta tcagactgct gtcttcaaat tcttcgggct
    51301 ctatcagcca acccttttcc agcctcctga acaatgcttc cttaatttct ttttttgtct
    51361 tgctgataaa gcttttcaat tcttccgtct tcttgtccgg cagctgtaca gcctcttcct
    51421 tcaaggaata gccctcaaga gaaatccttc caagcttcat tttcaaatct gacatttcct
    51481 catcaatttc tgattccagc cgtcttattt tatctttaac ttcatcaatc tcttctctgg
    51541 tttcatctat ctcctccgca acaccggcat catcctcctg aacatccgag agcttttctt
    51601 ttaattcatc cagctcatcc ttaaggcttt ctagctttcc ctccatacgc tccagctcat
    51661 tctgcaaggg cttaaccagc agaagtgtct tatctatttc ctctatatac atttgaatcc
    51721 cgggagtcag gttttcattg tttttcttta tgctgtcaaa ctcccgggac tgattctcta
    51781 caaactggcc ttcctcagcc ttaatgtttg tttcatccct gctttggata taatcagcaa
    51841 gaaggcctcc ggcattccca ttgcaaatta agctgttgat ttccaaggct cctgacagct
    51901 catcccttat atcctcaagc ttttccgcgc ttatatttgt cagcttcttc ttaatacttg
    51961 taagttttat atttacttca cttaccttgg ctctcagctc atttgcctta tctctggtaa
    52021 tcttctcact ttttgcaaat tcaatccctt tattcagctt aatattctgc agctgctcta
    52081 taaggctgcc ggttgctgaa attactgttc tgtatttcat atattcctgt acctgtctat
    52141 taaaagtctc atgcttcaac aggttttcca tgccttgtat ttcaatatcc tcataatcta
    52201 tacttatatt tataaagttt atattcttat gccgctcctt tagatttaca ttcaaatatc
    52261 tataaaagtc ctcttttaca tttccttctg cagcattcac tccgtatatt ccatagctgc
    52321 ccataagctc actgtcatag tctgccaata cagacctggc tgaggtgttc agtgtacttt
    52381 gaactctccg gtcggcagcg ataattctca caatatcaat tactgttcct gcaaaaatta
    52441 tcagagacat gaaaataagc gccagaaaaa cagtaattgc ccctttctct tttcttatgc
    52501 tgccatccct ttggtacatt tgcctctccc ctttgatgac caatcatctt aacgttttgc
    52561 ccctattctg cctgccagtt caccgaggtc aagttcctct ccaagcctct ttgataactc
    52621 cagtataagg tcaacattcc ttatatactc atccggctct gatactgctg cttcggattg
    52681 aacactcaaa gtcagagtat ccttaccgtc aaagaattcc tttatgccgc ctaaaggcac
    52741 cttaatctcc tggattaact caactatgag tcttccccta agagcgttgt tattgaatgt
    52801 aatccttatt cttgtacttt cgggcttcag gattgtgctt tcaatccttt tgcacaaatc
    52861 ctccccaatc agaagggctt tatgcccggg cagattattc cccgcatcca ttttaaaaac
    52921 caccctggtt ttgccattgt cagatgcttc tctttcaaaa tatccttcat aggttgtact
    52981 tgaaaacagc agattgtcaa aaattctgta gcccattgaa tcttcaggtc ttttattgtt
    53041 tattttccca tcctccatat ttcttctgct gtcaagccat aactccgccc cctgctgagc
    53101 agcaaaggag gcaaagcttt caaggcttgc cctttgcccc atgtataaga atgtaaacaa
    53161 aagtgccaat aaggccagca taatcgtcac aaacaccatg gaggcttcaa cagtatagct
    53221 tcccctgtca catatatgct tgtccagcaa tcatcacctc cgcacccttt tcaggaagta
    53281 aatatcctgt acaaggcgct agtccgtcgg caacggtgca gtcatgtcaa tttcgttatt
    53341 ttcaacagca ggggcagtat caataaaatt tcttataacc ctctgcacat aagcagttat
    53401 cgactttctg aagagcaatg ctattcctat aagaattgca attattacca caatctcaac
    53461 cgtacttatg ccatcctcct caacaataaa tccttttact tcctttaaca ttttgctgcc
    53521 tcctttaatt tttatttggt attcgtgtat catatttaaa gataccttaa ctgcattact
    53581 gcagggagtg ttacaataat aagtattcct acaaacataa tcatcagcgg aagcaccagc
    53641 ttggttgagg cctcttcccc cagcttcttc gccatattct ttctaagctg ccagcattca
    53701 ttcgattgaa gcttcagcag cgggaccaaa tcgctattgc cttttttcag gttttgtatc
    53761 acggcagtaa tgaatttggt tatttccttt accctgcatc gttttgcaaa atcctcataa
    53821 gcttccattt caggctttcc gttcataatc tcaatatagg ttgttcttag ctcttcatat
    53881 aggggagtga tattttttct gtccctgact atcttctccc aagccctggg tacagtcatt
    53941 ccggcattga taaggagcac catcttatta agaaaatcag gaaaatcgta tcggatgtta
    54001 aaattccttt ttttgatttt ttcatccaac tctctgtctg aaacataaaa aacaatcccc
    54061 agtgccgaca atgcaaatat tgcatagatg atatcaggct tttccattaa tgcccccagc
    54121 agagtaacag atagcagtgc aataatcatg tgaattacct tatgtacaaa atggagcttg
    54181 agcatgtgct ttatatccct gcctccataa agctcagcca gctttatgcc gagcttcctt
    54241 tcataagcaa tgaacctccc tgcaatgagc ttgtctgtta tgaataaagc agcaggcgcc
    54301 aaaactttgt atatatgccc ttttttaaag cctgtttcgg taaaatcttc atatttgccc
    54361 tttacataaa agcaaataaa tccaagaaca ataatttccg taataaatac cggcagcata
    54421 agctatacct caatatccat gatttttctc gatacaaaat aagcggctcc aaaaagcact
    54481 aatgaaaaag tcattgccgc tctgccaagg ggctccgaaa ataccggagc catgaattcc
    54541 tcagcgctgg cggacagcag aagcacgata agcaccggca tcaggttaag cactttatgt
    54601 tccagccttt tttccataag cagaagattt atctcctgcc ttatatcaat tttctcggtt
    54661 attatttccg cagtattctt tactacctgg acaagattac ctcctgtgcg cttgcatatt
    54721 agaaaaacat cggcaaagtt tattatatcc tccaaatgcg accttgcagc aaagtcagcc
    54781 aatgcttcct ctatggtttc attcatctca attcttctgc ttatatgctc aagctcgatc
    54841 aatatatatg tatcattgtt gggatactgt atggaaagct ccttaataga gcccttgatc
    54901 gcagtttcta ttgactttcc ggcagacaga gaagatgaca atgagtaaag ggcttctctg
    54961 aactgcaagt tgagttcgtt ttttcttttt tttattattt cttttgtcct gaatccggga
    55021 tacaaaaggg caaagggcgt taatagcagc gataacaaag cacttctgta gaaaatataa
    55081 cctgtcgcaa atataactgt acccgccata aatatgcata aaagcttttc ttttaatgac
    55141 attatataag tatcatagtc tgcgagagat gcatgtgcag cagtacccct tccatattcg
    55201 tactccgttg agactgccgg ccattttcta agatccataa aaaatcacct catatcactt
    55261 cattcaaccc tgccattctg agctttgcca ggtttttcat agtattgccg gttcttacaa
    55321 gacacccttc aaccttttct gacattgatc cgcctcgctc cacaaattca aagagaggat
    55381 taagccctat cattccttct ctgcagtctg ttacctccac tatttccact acttttctcg
    55441 tgctgtccct gaatcttgaa aggtgtatca taatatcaac ggctgaagca atctgctgcc
    55501 taatagcctc caaaggcatc attgcgccgc tcagcaccat ggtttcaagc cgtgagagca
    55561 tatcagcggt agaatttcca tggcctgttg aaattgaacc gtcatgaccg gtattcattg
    55621 cctgaagcat atcaaaggct tcagctcctc ttacttcacc aatgatgatt ctctcaggcc
    55681 tcatcctcaa tgaggccctt atcagatccc ttatggttat ttctccttta ccctcgatgt
    55741 ttgcatttct ggtttccagc ttgactatgt tctttatatt ctttatttgc agctcagccg
    55801 gatcctcaat ggtgacaatt ctttcatccc ggggaatgaa atctgaaagg gcgttgagga
    55861 aagaagtttt gcctgatccc gtacccccgc atatgaaaat gttatatttg gcctttacca
    55921 acctttccag gacttcggca gcctcttccg taatagagcc ccattcaatc aagtccttca
    55981 ttgatatggg cctttcagaa aacttcctta ttgttagtat aggcccgttc aatgcaatag
    56041 ggggaagtac cacatttact cttgatccgt ccgccaatct ggcatccgct atcggcgaag
    56101 cttcatttac gactctgttt accttcgaaa caactgattg tatgacgtcc tcgagcttct
    56161 gccggctttc aaagctgctt tcaatactgt atgtccttcc gtgtttttca ataaaaatat
    56221 tatcctcacc gtttaccatt atctcggtta tggacttgtc atcaataaga ggctgcagta
    56281 tatcaagctt ccgtatggaa ttgaaaagca cggatataag ttcctgcttt tccctaatgc
    56341 tcaaagaccg cagcctggcc tcctcaaaaa ccttatctgt tatcagttca tttatatctt
    56401 catcagtgac ttggctttta atgtccagac tttctcttat ttcttttctc aaattattta
    56461 tcaccaagtt tttcgcatca tcatccatgt actccacctc aacggccagt tataacaaga
    56521 tttcaatgag ggtttttgca tacttgcaaa aacctacccg agcgtttcaa cgaggcgaga
    56581 tatgctcact gcctgtgggg caacgaaagg ggttcccgtc acttgtagaa gtggtaacat
    56641 cctttcgcct cgttataata tcaataatcc ggttaacggc tttcctgaaa tcatcatttt
    56701 caataaaaat cctcccatcc tctctgatca gtgtccttga gtattcagga atcttaactg
    56761 ctgcagcggc attttctcca aagctttcga agtcttcatc ccctttgttt ttatacttgt
    56821 ttatcactgt aatgaattta tcggaaatgc ttccaccgtc cgtatcccgc agcttggcaa
    56881 gctcattcag caatactctt tctttatacg atgcaattgg ctcccgcaaa gtaacaagta
    56941 caatacggtc acataatttc attatcttgt aattttttga gtcaaaattg ctggacatat
    57001 cgataaaaac atagtcacag catcccatgc cttttatccc ttttataagc tgttccaatt
    57061 cctccggatt tatttcctcg tattccatag ggctatcagg aggattgaaa tattccaccc
    57121 catcatcagt actttttatc ccctccattt taaaggagag gttccggctt ttttctttga
    57181 tatagtaata aacataagaa agatttcttt tgctgcctaa accgaaaaac attccggtgg
    57241 attgaatgga ctctagattc aaataaaatg atttcaagcc cagttcggca cattgtgttg
    57301 ataaggatgt cgctattgta gtttttcctg taccccctgc aggagaatat acacctatga
    57361 actcagtatc ctttgaacag gatgaaagcc taatctcttg gggattggcc ctggagtaca
    57421 aatacagcaa atcccgaatc agcttgtctc cggtactgta ctttctgatg gcctgaaacc
    57481 cgggatactc cctgttcaaa gcttcccctg aaagtattac cttaagctta atattcggat
    57541 attcaaccga aatatcataa aaatcggaac tgattgccag aatatcggtt tgaggctgct
    57601 tctcaaaata tccggcaaaa gaatcaagct ttgtaaagca gctgaccatg aaggatgagg
    57661 aatgatttga attgatatat tcaaacagcc cccttgcata actttcgtcg caatctgcga
    57721 taaccagatt caatcttgcc atactcaaac tcccctcatt attatggctg ccgcagttcc
    57781 tgatgctata gctattgaaa aatgaaactt ccctccgtcc tgcctatttt ttaagtcggt
    57841 atattgaaga atatccatag tcagaaaaca acttttgagg tacacaaaaa gatgattgaa
    57901 tctttctgaa gcattttccc ttgtcagcat taggcctgaa gcaataaaac ctccaaagat
    57961 aaatgaatat attgcggcat ataaggcaaa tgctgcgccc attaccgctc ctacagcgca
    58021 taaaagcttt atatcccctg ctcctacaat ccttgtaata tataagacaa aaaggcatgc
    58081 ggcgggaatt ataatgccct gcagtgaaaa taccaggccc ccaatacctt ctattgcaag
    58141 atttactgcc agtcctatca gcatgaagcc gtaagtaatg taatttttaa cactgtaagt
    58201 cctgaaatca gatactaatg caagcagcat aagcattgaa actataatca gcttaaatac
    58261 tattgcaatc actcccaatt gcattgtata acatattaca ttttatacaa attttatatg
    58321 attttgttaa attatagcat atactagaaa tttttgtata tatatgatat atttggtatg
    58381 tcctattata cgaatgaata atacagtcta tttactgtat ttttacatcc cccactgtgc
    58441 aggaaaatat ttttcctgca taataattgc aaataaaaaa gccgcaataa atgcggcttc
    58501 ttatacctgg tatgacttac tttatttcta tctttgcgcc tgtttctgca aactttgcag
    58561 ctatttcttc tgcttcctgc ttgcttacag cttcttttac aggctttgga gcaccgtcaa
    58621 ccagatcctt tgcttccttg agcccaagac cggtaacttc tctaacaacc ttgataacct
    58681 tgatcttttc tgctcctact tctgcaagaa ctacgttgaa ctcagtctgt tcttctgccg
    58741 gagctgctgc tgcacctgct gctgccggtc ctgcaactgc tacaggagct gctgctgtta
    58801 cgccgaattc ctcttcaatt gccttaacca attcagccag ttcgagaact gtcatattct
    58861 ttatttcttc aataaacttt aaagtctttt cacttgccat tttaaattac ctcctaattt
    58921 tttaattaag cttccgcttc tttcttttct ttaatagtat taagcgcata tgcgagattt
    58981 ctgattgaac cctgtaatcc tccaaggagc attgcaatga gcacttctct tgatggaagg
    59041 tctcccaagg cctttacttc atcaacgttc ataatcttgc cttcaatata tccggctttt
    59101 attgatatcg gcgtcttggg attatcctta acaaactgat tgatggtctt cgcgggtgca
    59161 actgcatcgt catatccgaa agcaactgca actgtgccct gaagatattc atgaagcgct
    59221 tccatgccca attctgatgc tgctcttttg aaaagggtat ttttgtacac cttatagtca
    59281 acgccggcct ctctgaacct ttttctcaaa tcggtcacat cttcaacact tattccctta
    59341 tagtccacca atacaactgc attggacttc tgaagctttt ctttcacttc acttacttgc
    59401 tgttttttct gttcaaggtt ttttgccatt tttacacctc ctcatggtaa agcttaaatc
    59461 tctcttatgg ataaggcctt cataaaatca aaagacctct ccgtagacat agagaggtca
    59521 taatatcttt atgacaaacc tcggcaggaa tttacggcct gagccaacct actgtctacg
    59581 gtatatattc gtttcaaaca cgggcactaa tcggttacgc gggtaggatt gatttttata
    59641 gccggtccca tagtgcttgt aactacaacg ctcttaaggt aagtaccctt tgcagctgcc
    59701 ggctttgcct ttataactgc ttccataaga gtcctgaagt tgtcaagaag cttttccgta
    59761 ccgaaagaga ccttcccaaa accaacgtga actattgatg tcttgtctac cctgtactca
    59821 accttaccgg ctttgatttc attgatggct ctttcaacat caaaggtgac cgtaccggat
    59881 ttcgggtttg gcattaggcc tttaggtcca agtatcttac caagtcttcc gactacaccc
    59941 atcatatccg gagtagcaac taccacgtca taatcaaacc agttttcacc ctggattttt
    60001 gcaaccaatt cttcagcacc tacatagtca gctcctgctg cctctgcttc cttggccttg
    60061 tcgccttttg caaataccaa gaccttaagg gtcttgcctg taccatgggg aagcacaaca
    60121 actcctctga cctgctggtc tgcatgtctt ggatcaacac caagctttac gtgaatttca
    60181 acagactcat caaatttagc ctttgctgtc tgctgaacaa gctccatagc ttccttaggg
    60241 tcatataatt tggttctgtc aataagcttt gcactttcct gatatttctt tcctctaaac
    60301 attacaaatt cctccttgtg gtattagcgg aacatgtcct cccactgata ttaatcttct
    60361 actgatatac ccatacttct tgccgtaccg gcaaccatgc tcattgccgt ctcaatgctt
    60421 cctgcattca agtcaggcat cttcatctct gcgatttctc tgaccttggc ttttgaaatc
    60481 tttcctacct tggtcttgtt cggtatacct gatccgcttt cgataccggc cgctttcttt
    60541 ataagtaccg atgccggcgg agtcttgagt atgaatgtaa atgatctgtc ctgataaaca
    60601 gttattacta caggaataat aagtcctgcc tgtttagccg ttctttcatt gaactctttg
    60661 cagaatccca tgatgttgac accatgctga cctaaagccg ggcctattgg tggtgccgga
    60721 gttgctttac ctgccggaac ctgcagctta accaatgctg aaaccttctt tgccattatt
    60781 acacctcctc tcagcaaatg atatatgtct atccgggtct atagcctttc aatctgagta
    60841 aactccagtt ccacaggagt ctccctcccg aacatcgaaa tcataacctt taccttatgc
    60901 ttctcgttat tgatctcttg tattacaccg acgaaatttt caaaaggccc ggaaataact
    60961 cttatatgat ccttaacctt cgcatccaac ttgattggaa tcttttcaac gcctaaattc
    61021 tttatttccg catcggtaag aggcacaggc ttagatccag gtcctacaaa tccggtaacc
    61081 cctcttgtat tccgaacaac ataccatgat tcatccgtca taatcatctt gaccagtaca
    61141 tatcccggaa atactttttt ctgggttatt tttttcttac cgcctttgat ttcgacctgt
    61201 tcttctaccg gcacttttac ttcgaagata acatcctgca tgtttctgtt ttctactatc
    61261 ttcaaaatat tagccattac cttgttttcg tagcccgaat aagtgtggac tacataccat
    61321 ttgcctcttt cttccataga tgcacaaccc taaagtatct ttaaggtttt cctcctaatc
    61381 aataatatgg ttatacagaa gcccttctat ttaattaaaa ggttaagcac tttcccaaat
    61441 ataaagtcta tcacccaaag aactgctcct accagtataa ttgaagtaat aaccacttcg
    61501 gtatgctgta aaagctcctt cctgttgggc caggtaacct tcttgacttc tgcttttgtc
    61561 tctttaaaag attttgccac atcaaatctt tttgctgcac cagccatgcc attcacatcc
    61621 tttacaggct atttcgtttc cttatgcgat gtatgcttcc tgcagaatct gcagtacttg
    61681 ttcatttcca ttctatccgg gttgttcttt ttgttcttca tggaatcata gttcctttgt
    61741 ttgcattcgc tgcaagcaag tgttatttta actctcatat ctacacctcc caacgacttg
    61801 gcactacaat actgtataat aaagatacta aaatgccgca aaaatacata aaaattgcat
    61861 tttgagacta ctcaataaaa tctatcataa tttaacttca atgtcaataa tattttgtgc
    61921 cctccctcat gcataacagc agtatacatg agacgttgcc ctgcttatag tattgcctgt
    61981 tttagacatg ttaatcgcaa taagcccaat ccggcttaaa tctgcaatta agttttcagg
    62041 tattatgctt tttaaaggac taggatcaaa gacctagtcc ttagctaact ctgcactctg
    62101 aagctcttct ctgagatcct gcgaataggc gcagcctatt cgattaccga tgcaacagca
    62161 cctgatccga ctgttcttcc gccttcacgt atagcgaatc tcaagccttc ttctattgct
    62221 atcggagtga taagctttat tgtcatttct atatggtcac cgggcatgca catttctact
    62281 cctgccggta attcaattac tcccgttacg tctgttgtcc tgaaataaaa ctgcggcctg
    62341 tatccgttga agaatggagt atgccttccg ccttcttcct ttgtcagtac gtatacctgt
    62401 gctttgtagt gtgtatgagg ctttattgat ccgggctttg caagtacctg ccctctttct
    62461 atctcattcc tctgtactcc tcttaacagt actcctatgt tgtctcctgc ctgtgcctgc
    62521 tcaagaagct tcctgaacat ttccactcct gttactaccg tggatctcgg cgcatctgca
    62581 agccctacta tttctaccgt atccgatacc tttactgttc ctctttctac tcttcctgtc
    62641 gccactgttc ctcttcccgt tatcgagaat acgtcttcca caggcataag gaacggcttg
    62701 tctgtctgtc tctgcggtgt gggtatatag gtatctactg catccataag ctcatgtacg
    62761 ctcttgcacc attcacagtc ttcttttccg catccgcact ccagtgcctt taatgccgat
    62821 cccgctataa tcggggtatt atctccatcg aattcatact ctgtaagcag ttcccttact
    62881 tccatttcta caagttcgat aagttctgcg tcatctacca tgtcaacctt gttcagaaat
    62941 accactatgt agggtactcc tacctgacgg gacaacagta tgtgctctct tgtctgaggc
    63001 atcggaccgt ctgccgccga tacgacaagg attgctccgt ccatctgtgc tgctcctgtt
    63061 atcatgttct ttacatagtc agcgtggccc gggcagtcaa catgggcata atgcctgttg
    63121 gctgtctcat actcaacatg ggctgtgttt attgttattc ctctttcctt ttcttccggt
    63181 gccttgtcta tttcgtcata ctttgttgca gttgcctgac catactttga aagcacaact
    63241 gttatagctg ccgtaagtgt agtcttgcca tggtcaacat gacctattgt ccctatgttt
    63301 acatggggtt tggtcctttc atattttgcc tttgccatta tacttctcct cctctaagtt
    63361 tcaagattat aggttaaacg tgcagacagc ccgaaactat ctgtacagca tactcgccgg
    63421 ctccaatccg gctgctcact aaagccgcca tagagttaca gcaaactcat ccgtgtgtgt
    63481 ctatataaag atggagctca cgagcgggct tgaaccgccg aacctcttcc ttaccaagga
    63541 agtgctctac ctactgagct acgtgagcat aacacattat attggagcgg gcaacgggaa
    63601 tcgaacccgc atagccagct tgggaagctg gtattctacc actgaactat gcccgcaaat
    63661 ggtggagagg gatggatttg aaccatcgaa gtctgcgaca acagatttac agtctgcccc
    63721 ctttggccac tcgggaacct ctccgtacaa tatggtatta taatgctaat ttatagtttt
    63781 gtcaatatgg ttaaagccta gagaaatata taactcaaga gtatattaca tgcaattaat
    63841 gtgacattta atacttttta tataatgtcc gggcgggaat atatgcttgc ttttaatttt
    63901 gtttcatcat aaatattatg aaacttttta taatatttat taacgcattt tgataacaca
    63961 tgagaaagca caaatttccc ttgtaatgct tctctgaatg tattacacaa ttgctgaaga
    64021 gaggtattga aattaaatat atattgggtt acaataatag tgtactatat tatagaatgt
    64081 tgttcttcat tatagtacgt tttggcgggg agggatcgta ttgaataaat ccggtgaaaa
    64141 tgtaatacag tcagtatgca gagcaataca aataattcgc tgttttgaaa aaaatgaaga
    64201 actgggagtt accgaaataa gcaaaatggc tgggcttcat aaaagtactg cttttggttt
    64261 aatatccaca cttgaagcaa acaaattgat tgaaaagaat gagcttaccg gaaagtacag
    64321 attggggctt gaattgttca ggctgggtac caaggttaat tccgccctta ggcacatagc
    64381 cgtaccatac ctggaacagc tggtaaaaat gtatcaggaa acagcaaact ttgttgttat
    64441 ggacgacctt tccattatct accttgaaaa agttgagagc acacagtcaa tgcgtatcag
    64501 caccttggta ggaggcaggc tgcctctgta ttgtactgcg gtgggaaaag cgatattggc
    64561 caatcttccc ccggaggagc ttaacagcaa actcaaaagg atggaattca gaaaatacac
    64621 cggcaatacc gtttctgaca gaaactcgct gatgaaatcc cttgaattaa caagaaacag
    64681 agggtatgca gaggataacg aagagcttga gatagggcta agctgcatag ctgcacccat
    64741 ttataaccac ttcaatattc cctttgccgc tataagtctg tccggcccaa cctttagaat
    64801 ggatgagatc ttccgaaagg aagcaagtgt gacattaatg aattacactg gggaaatatc
    64861 tatgaaactc gggtatgaca ataataatct ataaaatatt aaaatttaag gaggaattca
    64921 aatggcaaag aaaaaatcaa tcaacgactt ctatgaaatg aagaagacgg gggaaaaagt
    64981 ctcatggatt accgcctacg attttccgat ggcctcattt gctgaacagg ccggcatgga
    65041 catgatcctt gtgggtgact ctttaggtat ggttgtattg ggatatcaag gtacagttcc
    65101 ggttacaatg gacgattgca tatctcactg ccaggctgtc agaagaggtg ctccgaatac
    65161 ttttgcaata ggtgatatgc cctttggttc atatcaggta tctgatgaag aagcagtaag
    65221 aaacgcatgc agattcctga aggaagccga tatggatgcc ataaagcttg aaggcggaat
    65281 tcgtgtacta agcaggatta aagcaatttc tgacgcagga atcgttgtat tcgggcatat
    65341 aggcttaaca cctcaaagct caggacagtt gggaggattc aaagcacagg gaagaacagt
    65401 tgacagtgca agagaattga ttaaggatgc cctcgcaatt caggaagcgg gagcaagagc
    65461 actgcttctg gaagcagttc ctccggaatt gacagaatac ttaagccata aattgactat
    65521 cccggtatat tccattggtg caggacttcc ctgcgacggc cagttaataa tatgcgggga
    65581 tatgctgggc cttttccagg ccttcactcc aaaattcgtc aaaaaatatg ccaacgtagc
    65641 tgaagttatt attaatgcat tcaaagaata cgtggcagat gtcaaagagg aaaaattccc
    65701 cgaagacaac catgtatacc atgcaataga cagcaaggaa gcctttgaag ctttattcag
    65761 ggaatttgag taaataaaca ttgacatctt gcagatattt acatagaata aaatatgaaa
    65821 gtcgtagaaa tcaataattt gataaacgcc gaaaattaaa gttcgaggta ttttatgtct
    65881 gagagtgttc ataactagat aaatgaatct agcatcgaaa gggaatccta ttaattgctt
    65941 tgcgcattaa taggcttggt tagttgccct tttgatatta tggacacgga accaattgta
    66001 tatgttgtac agtgccgtgc ttttgcacgg ttttttcata tttggcgttt atctgcagga
    66061 tacgtatgac tgtaccggat ataaaggttg tgattggacg tagtctgatt acggcctttt
    66121 cccatttaag gaggaatata aattgaattc aacagctata aaaattttag aatttgataa
    66181 aatcaaggaa ctgctgaaaa cctatacagt atccaatctg ggaaaggatt tgacagataa
    66241 gcttgaacca tctctgaata ctgacaccat attgtcgtgg atgaatgaaa ctaccgaagc
    66301 ttgtgccata ttgcagagga gcggcagcgt accaatccac agccttaacg gtatagaaag
    66361 tatcgttggc aagttaggaa agaatatgac tctgcttccc gaagaattcg aagtaatact
    66421 tggattgctt caggaggttg gcaagcttaa gcggttcatg gaggataaat ctgcaatagc
    66481 ccctatagta agcagctttg ccttgtccgc ctatgagctt gaggagttgt ccgaggaaat
    66541 agaaaggtgc attcagaacg gagcggttgc tgataaggcc agcagcgagc tgtccagagt
    66601 aagaaagaaa ataataatac ttgaagacag gataaagggc aagcttgaaa gcattttgag
    66661 atccccggcc tatgccaggt atcttcagga cggaataata agctcccgaa acggcagata
    66721 tgttataccc gttaaaagag agtatcgcag gaatgtagac ggggaagtga tggacagttc
    66781 agtcagcggg tctactgtct tcattgagcc ttcggaagta agaaagctcc aggacgaact
    66841 gaatcactgc aggatagagg aggaaaaaga agtctgcagg atattgtcaa tgctcacaaa
    66901 tcttgcagaa tcatacaaaa aggaaataac aaccaatata caggttatgt catattatga
    66961 cttcctgttc gccaagggaa ggttcagcag atctctggaa gccaaccctg taagcgtcaa
    67021 tacctgcggc aggctggaca tatgcggagc caggcacccc ttaataggaa agtcagc
//
LOCUS       DAOQUR010000009        66301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_489_length_66301_cov_10.828065, whole genome shotgun sequence.
ACCESSION   DAOQUR010000009 DAOQUR010000000
VERSION     DAOQUR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 66301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66301
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_489_length_66301_cov_10.828065"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..601)
                     /locus_tag="PKI50_03315"
     CDS             complement(296..601)
                     /locus_tag="PKI50_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT02774.1"
                     /translation="MEKKYKIEYLPSSAKDLAEIVDYIKIDSPQSALNFLDKIDESIS
                     RLEQFPFIGVIPKDIRLQSLGYRILIIDNHLIFYVVFDDIVEIRRIISGKRKYDFLF"
     gene            complement(585..860)
                     /locus_tag="PKI50_03320"
     CDS             complement(585..860)
                     /locus_tag="PKI50_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNT02775.1"
                     /translation="MPIIRPISDLRNNFNDISEICHTESEPVFITKNGQGNLVVMSMA
                     LYEKQQALIELYQKLSVAEAESKYSDNRISHKNLMKELRETINGKEV"
     gene            1314..1673
                     /locus_tag="PKI50_03325"
     CDS             1314..1673
                     /locus_tag="PKI50_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02776.1"
                     /translation="MSQNNKSLNKIKWAARISAMVIIAFGLPFYFGYGNPLPFINPDY
                     TLWDNMWLMIFPIMFIGLALGLKFEKTGGYLVIISVGLGLLMGLFMQGELVSHMLVPL
                     LVGVLYLIVGYKSDKNY"
     gene            2098..2889
                     /locus_tag="PKI50_03330"
     CDS             2098..2889
                     /locus_tag="PKI50_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT02777.1"
                     /translation="MKIISIISSSRKNGNTENLVSILEEELKSVSELEHEEIEIERIY
                     LGQANLKMCMGCRICFDKGEQFCPLKDDLLTIREKVVEADGVILASPVYVEDVNGIMK
                     NWIDRMAFYCHRPALAGKVAVTITTSGAGSTNHALKTISFAIMSWGAYLGGQYKFRTG
                     ALMKREEIYEHNHSKVNEVARKLIDDIKNQKAFMPTLYSLIIFKVQQKLWQKNTDSQN
                     SIDYTYWNNKGWLEPKCVFYTTIRTNWFKVKIARLFGAIIALFFK"
     gene            3285..3650
                     /locus_tag="PKI50_03335"
     CDS             3285..3650
                     /locus_tag="PKI50_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02778.1"
                     /translation="MKSLFSKLGFRKMDEMEKNIALKAQQNALIYVLLALAMWSFYES
                     FKAYSQHTSINSIPSFLLVTTSLVQVFSQLVLQKRVVKDDDEYKDDNPLLKIIIVGVI
                     ISAIIVSIGALLIVSGIQK"
     gene            3647..3838
                     /locus_tag="PKI50_03340"
     CDS             3647..3838
                     /locus_tag="PKI50_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT02779.1"
                     /translation="MKNKIKQLRKNKGLRQEDIAMLLNVSRQTIVAIENDKYNPTLEL
                     AMKMARLLDTTVEELFILE"
     gene            4086..4631
                     /locus_tag="PKI50_03345"
     CDS             4086..4631
                     /locus_tag="PKI50_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02780.1"
                     /translation="MINEREKVIKAKEVLIKIANGINPVDGTPINEESFLNDPRIIRC
                     FFYISELLNRVIAGQTNQNLPKPTELHITKEEKSLVKFPENKIGVNEFAKCINNVIDL
                     NKSKKLSGVELNKQLKKMGVLSENIDEQGKVRTIINNKSVDYGFESEKRNYNGNEYEM
                     VVINDIGKAYILENLEDIMKY"
     gene            5048..5341
                     /locus_tag="PKI50_03350"
     CDS             5048..5341
                     /locus_tag="PKI50_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02781.1"
                     /translation="MGITPFKSSGRGPAILRNCILGVWVLMFAFKQSTTFMARNSGEV
                     TEIVAGLLIIFFSLYDTYVRREKNDVWGIPTWILGGLILTIGILFYLYKNPFA"
     gene            5466..5927
                     /locus_tag="PKI50_03355"
     CDS             5466..5927
                     /locus_tag="PKI50_03355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT02782.1"
                     /translation="MTVRIAKLSDIDVIYDLICDMENSKLDYERFQEVFKKYLEDDRY
                     FCLVAEHSGTVIGCLNLRIEYQLHHTAKIAEILELAVMDKYRSKGVGKKLFEKASEIA
                     KDNECLQIEVCCNQLRSRAHNFYEKQGMHNFHYKFSLNFKEEEIKENRLGL"
     gene            6484..7212
                     /locus_tag="PKI50_03360"
     CDS             6484..7212
                     /locus_tag="PKI50_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT02783.1"
                     /translation="MYTFTNQFMSNNKNVEFLKANMMGPNAMRLAEELASHLNINENM
                     RILDLGCGCGLSTLLLVKKYGVSVFAADLWISPTENYERFESVGIDEKAVPIFSDATK
                     GLPFANGYFDLLFTVDAYHYFGDTAEMLPSLIPFVKKEGHIAVAIPGLKYEFGENVPD
                     EMQPFWDSDMERTLHSLDWWKDLWKSAKGIEVMDIREMGCCRQAWEEWLASDHPIAAE
                     DIKMMEAEGGKYFNIIQLIAKVKR"
     gene            7247..7690
                     /locus_tag="PKI50_03365"
     CDS             7247..7690
                     /locus_tag="PKI50_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT02784.1"
                     /translation="MEVKPIKGNKKLFLDLLLLADEQEDMIDKYLERGEVFALYDGDL
                     KSICVVTQEDENTCELKNLATYEKYRGKGYGKYLISYIFDYCRDKCKTLLVGTGDIPW
                     IISFYEHCGFEVSHKVENFFTNNYDHPIYEDGIQLVDMVYLKKDF"
     gene            7809..8261
                     /locus_tag="PKI50_03370"
     CDS             7809..8261
                     /locus_tag="PKI50_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT02785.1"
                     /translation="MEVISIKKSPEYKDRAIKYLQSKWASPNSMKVYDDCITNCITTL
                     SPLPQWYLLIDNDRIIGCAGLITNDFISRMDLYPWVCAVFIEEEYRGNAYGSLLIEQA
                     KIDAKAGGFSHLYLCTDHIGYYEKYGFKYIGTGYHPWGDSSRIYDAAL"
     gene            8425..9177
                     /locus_tag="PKI50_03375"
     CDS             8425..9177
                     /locus_tag="PKI50_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002066342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNT02786.1"
                     /translation="MRLVEIERFIISLFESSENEHFHEQSGTTVEGTEEIRRIGYCTN
                     LTLDTIEEGKKNNVDLIITHHDAWDFIFGLKDACREKLSEYNISHYFTHLPLDDCNFG
                     TNNSLIEKLGLSIIKKSHNEDGFYCGRIAEFDEEVEFEELVRKLEMILGEPTKAWKFK
                     DGCIKRVGLVCGGGGSTADVREAFEKNCDVYITGEKVLYTIEYAKFVGMNLIIGSHTF
                     TELFGIESLARKIADKYRGVNIIRLSEEHLEA"
     gene            9237..9620
                     /locus_tag="PKI50_03380"
     CDS             9237..9620
                     /locus_tag="PKI50_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT02787.1"
                     /translation="MKAQINLITIWADDINKMKNFYNQVLGFKISNDLGNYVEFENEG
                     VRFAICLREVMYAYSSEYEKKTGGQAFELAFPCEDPEDVDKSFEWLISKGATPIHQPQ
                     NMPWNQRTALFADLEGNIHEIFAEL"
     gene            9636..9869
                     /locus_tag="PKI50_03385"
                     /pseudo
     CDS             9636..9869
                     /locus_tag="PKI50_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419935.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            9966..10367
                     /locus_tag="PKI50_03390"
     CDS             9966..10367
                     /locus_tag="PKI50_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNT02788.1"
                     /translation="MRSELIAPCGMNCRLCMAFQRKKNKCIGCNSDDCTKPAYCLHCI
                     IKNCVTIQNSSSGLCYECDKFPCRRLKQLDKRYRTKYHMSMIENLENIKQNGMKEFLK
                     NEEARWACIECGNIVSVHRNACPICKTQYIE"
     gene            10469..10600
                     /locus_tag="PKI50_03395"
                     /pseudo
     CDS             10469..10600
                     /locus_tag="PKI50_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
     gene            10625..10975
                     /locus_tag="PKI50_03400"
     CDS             10625..10975
                     /locus_tag="PKI50_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNT02789.1"
                     /translation="MSASVKPNITIDILDKIDIRVGTIISVEDIKDSNKLVKLSVDFG
                     DFLRTILVGMKGERENPKEIEGQQALFVVNLAPKKMAGELSEGMLFDIGYADGIVPVL
                     AQPEKAVPNGTRAG"
     gene            11037..11879
                     /locus_tag="PKI50_03405"
     CDS             11037..11879
                     /locus_tag="PKI50_03405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT02790.1"
                     /translation="MFNNLKAINERPKPFQYYTAAELWTDEHTSKKMLDYHLNEEIDV
                     SSRNARFINRSVSWIIEKFNLNNKSNVLDFGCGPGLYTNRFAERGINVTGVDFSKRSI
                     EYAAQIASSKNLKVNYVHKNYLEYETQDRFDLITMIMCDYCALSPEQRGIMLNKFKEL
                     LKHSGNVLLDVYSLKSYNRREEIAAYEFNHLNNFWSPEDYYCFVNTFKYCDEKIILDK
                     YTIIEKAQTRVIYNWLQYYSVETLRREFEDNGLEVLEFYSDVCGLPYDAESLEFAVAA
                     RQKN"
     gene            12129..12980
                     /locus_tag="PKI50_03410"
     CDS             12129..12980
                     /locus_tag="PKI50_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HNT02791.1"
                     /translation="MTKGEKEKMSSGDYYSAVDEELVRERDYARNLTYEFNHTRPTEK
                     ERRLEIIVLSLLPGILILAFYLLVSWYLVPFSIPSILVIFVAIAVILVPFELGFLIYQ
                     GRKVNKRISLNNVVLFREKVPVIQFIILVVILIKWSEFVFRCLSTRIDTLFVNNFFYW
                     VPKWFFLDSFYQHISQYSKTVLLVTWILGLIFNGIIGPIVEELYFRGYLLPRMKHMKG
                     WAPIINVLLFSLYHFFTPWQNVTRIIALLPLVYVVWWKRNIYIGIVTHCALNITGMIG
                     MLPIVLK"
     gene            13068..13562
                     /locus_tag="PKI50_03415"
     CDS             13068..13562
                     /locus_tag="PKI50_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT02792.1"
                     /translation="MVEIIKASEEYAESFYQALDVVARERKYLGIVKGFPLQGVKDFV
                     SMNVANDYAQYYAIDGNKVIGWCDIIPKRNEGFTHVGILGTGLLSEYRNQGIGSKLIE
                     KAMEHAVKKNGIEKVELEVFESNINAIRLYEKFGFVYEGKRIKSRKLDGVYDNVVMMG
                     KEVL"
     gene            13563..14129
                     /locus_tag="PKI50_03420"
     CDS             13563..14129
                     /locus_tag="PKI50_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02793.1"
                     /translation="MKTNTMFSDVEIKLKKRNKILFSRDSKCLQELIKLIQLQNHRTL
                     VMWALDCAKLPLEQFEAKYPDERRPRICLELCEAWARGKIKMPTAKKAILNSHAAAKE
                     INDSEYGALCHAIGHAGATVHVGTHALGLPMYELTSIVLRYGKDNFLIPVSEKINYYY
                     KRLLYWQENTDKLKLDWADFLLRGDFNE"
     gene            14122..14652
                     /locus_tag="PKI50_03425"
     CDS             14122..14652
                     /locus_tag="PKI50_03425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT02794.1"
                     /translation="MNDYQIGRPKPEEICSINEFFEIVVRDTFAKNGISHLTELMEGE
                     IECKKKYLMQDMDSEGRDRFFLVVRDKGRIIGTIEYGRPNELIMKCTKGRYIDVIEIG
                     TVFVHPDYQYKGIGSMMLRMMYDELKNKGYTEFCLDSGYKTAQKVWTGKWGKPSCIME
                     DYWGKGAHHLVWRVDL"
     gene            complement(14665..14847)
                     /locus_tag="PKI50_03430"
     CDS             complement(14665..14847)
                     /locus_tag="PKI50_03430"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HNT02795.1"
                     /translation="MLLFYLNTYNKNSISSLTLIPSSGGVLKVKLNNELIFSKKSAGR
                     FPKEDEVEKLPIARLQ"
     regulatory      15078..15179
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15237..16688
                     /gene="guaB"
                     /locus_tag="PKI50_03435"
     CDS             15237..16688
                     /gene="guaB"
                     /locus_tag="PKI50_03435"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNT02796.1"
                     /translation="MEKFVKEGLTFDDVLLVPAKSEVLPKNTDTSTMLTRKIKLNIPL
                     MSAGMDTVTESKLAIAIAREGGIGIIHKNMSIEKQAMEVDKVKRSEHGVIVDPFHLSP
                     DHVISDAMELMERYRISGVPITDEKGKLVGILTNRDLRFETEFSKKISEVMTKENLIT
                     APVGTNLEQAEQILKKHKIEKLPLVDDKGYLKGLITIKDIEKAIQYPNSAKDPGGRLL
                     VGAAVGVTEDMIERAEALVSSKVDVVVIDTAHGHSKGVIEAVRRIKGRFPELQLIAGN
                     VATAGAVKELIEAGADAVKVGIGPGSICTTRVVTGVGVPQITAIYDCAAEADKYGISI
                     IADGGVKYSGDIVKAIAAGASAVMLGSLFAGTAESPGEMEIYQGRSFKVYRGMGSLGA
                     MACGSKDRYFQEDARKLVPEGVEGRVPYKGPLAETIYQLVGGLKSGMGYCGTHTIEQL
                     RKNTQFMRITAAGLRESHPHDVSITKEAPNYSL"
     gene            16779..18311
                     /gene="guaA"
                     /locus_tag="PKI50_03440"
     CDS             16779..18311
                     /gene="guaA"
                     /locus_tag="PKI50_03440"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNT02797.1"
                     /translation="MADKELVLILDFGGQYNQLIARRVREANVYCEVVPYDYSLDEIR
                     KKNPKGIIFTGGPASVYEEEAPKCDPGMFEMGIPILGICYGAQLMAHMLGGKVARAVK
                     REYGKTQVKLLENTLFREVPQDSICWMSHTVYVDSPPAGFDIIGATETCPVAAMGSKS
                     GKLYGVQFHPEVEHTEYGKQILRNFLYDICGLSGDWTMGNFIDENVERIKELAGDKKV
                     ICALSGGVDSSVAAVLVHKAIGNRLTCIFVDHGLLRKNEAEQVEEVFRKRFDINLIKI
                     NAQDRFLDKLKGVSEPERKRKIIGEEFIRVFEEEANKLGEIDFLVQGTIYPDVIESGT
                     KTAAVIKSHHNVGGLPENMRLKLIEPLRELFKDEVRLVGLELGLPEDLVWRQPFPGPG
                     LGIRVLGEITKEKLHIVKESDAILREEIKNAGLERCIWQYFTALPDIRSVGVMGDERT
                     YSHTVAIRAVTSSDAMTADWARIPYEVLEKISNRIVNEVEHVNRVMYDITSKPPATIE
                     FE"
     gene            18510..20063
                     /locus_tag="PKI50_03445"
     CDS             18510..20063
                     /locus_tag="PKI50_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNT02798.1"
                     /translation="MSYSPSLGSSLTNTKMRTPEHISDFSGMCSVCTASCIGTCEIGL
                     SAVRGSEAIYPFETDKNQFASEKSYPLDFSHFNINGRVFGALGCPEDANEATFPKANI
                     AVDFGINNKIKLKAPIILPAMAKLNWKDYYAGAALSGVLVVIGEDAVAKDKELVLENG
                     KVAGSPLLKEMVSSFRQYYRGFGDIILQGNYDDENLGVLDYAIKELGVKTVELKFGQA
                     AKGIQGMGRVKDIDAALEYQKKGYLVYPDPSDPEIAKNYKEGKGQVFEKIGKLPMWNE
                     DILINRVKELRKLGAERICFKTGPFDPKDLIRILKIASKAGVDLVTFDGAGGGSGNSP
                     VKMMNEWGIPTVYLESLLYNILKRMKEKNYNLPQVAITGGFAMEDQVYKGLALGAPYI
                     NIVGIGRAAMAAAMAGKQIGDLMKEGMTPKEYLRFGTAKEEVFGDIRELKLLYGEDAL
                     NISTGAVGLYSYINRIAAGLKQLMALNRKFSLEYIDRSDIIPLTEIASKVTGLEAVYR
                     YNNELDKEI"
     gene            20152..20400
                     /locus_tag="PKI50_03450"
     CDS             20152..20400
                     /locus_tag="PKI50_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNT02799.1"
                     /translation="MPGRDGTGPMGAGAATGRGLGFCSGVNAAGLGFGCRRGFVRGYG
                     KGFAVNWASAKTQKEMLQTQKDLLESRLKIVNEQLENI"
     gene            20463..20816
                     /locus_tag="PKI50_03455"
     CDS             20463..20816
                     /locus_tag="PKI50_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNT02800.1"
                     /translation="MPRPRKWRKVCCLPESNRFGPLGSPADESNYIVMTVDEYETIRL
                     IDLEGFTQEECASRMNIARTTVQGIYVDARKKLAESLVNGKVLWIEGGEYRLCDGSGN
                     ACGRGGCRRQRFGSK"
     gene            20831..21199
                     /locus_tag="PKI50_03460"
     CDS             20831..21199
                     /locus_tag="PKI50_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT02801.1"
                     /translation="MKIAIPVDDKNMEASVCISFGRTPYFLIHDTESNRSLFFENSAA
                     ASQGGAGIKAAQFLVDHKADILLTPRCGENAAEVIKAANIKMYKTIPGSAKDNVNAFT
                     EGKLSLLEEIHAGFHGHGEK"
     gene            21201..22061
                     /locus_tag="PKI50_03465"
     CDS             21201..22061
                     /locus_tag="PKI50_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT02802.1"
                     /translation="MKIAVLSGKGGTGKTLISVNLAAAAGKSVYLDCDVEEPNGHLFF
                     KPENFYEEEVSIKIPVADEKQCSGCRVCVDFCKFNALAYIKDRLVVFDEVCHSCGGCI
                     LLCPEKALNEKEKIIGKIQKGVSGQVTVNTGILNTGEASGIPLIKKLLDENRKVKGMT
                     TFIDCPPGSACIVMESIRDADYCILAAEPTLFGVHNLNMVYELVKLFRKPYGVVLNKC
                     QEGENPAERFCEEKGIRILGRIPFDNELGTLNSNAKIAVRESEKYRTVFSSLLETVTK
                     EVRHEAAVNP"
     gene            22039..22914
                     /locus_tag="PKI50_03470"
     CDS             22039..22914
                     /locus_tag="PKI50_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT02803.1"
                     /translation="MKQLLILSGKGGTGKTTIAGAFIKLSHAKAYADCDVDAPNLHLI
                     MGRNSEPEKRDFYGLPKAEINQELCIRCDRCRQNCRFEAILSDKAYKVDYFACEGCGV
                     CEAVCPAGAVSLNDAVAGELMLYSDEDKVFSTAQLKMGSGTSGLLVTEVKKQMRLNAK
                     DPELAIIDGSPGIGCPVIASLSGADMVLIVAEPSLSGISDMERIEKTAATFQTKAAVC
                     VNKYDANIANTARIEEYCRTNNLPFVGRIPFDPEAVKAINEGLTVIDIDCPAGNAVRE
                     VFNKTTKLLFDTSTI"
     gene            22939..23778
                     /locus_tag="PKI50_03475"
     CDS             22939..23778
                     /locus_tag="PKI50_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNT02804.1"
                     /translation="MSEQCNQNCSSCSESCTERKEEFDFSEKPHALSSIKKVIGVVSG
                     KGGVGKSLVTSMLAVTMNRMGYHSAVLDADITGPSIPKAFGIREKARGSEKGLYPIKS
                     HTGIDIMSINLLMDNDKDPVVWRGPILANTVKQFWSEVIWSDVDFMFIDMPPGTGDVP
                     LTVFQSIAMDGIIIVTSPQELVSMIVSKAVRMAEIMNIPIIGLVENMSYFKCPDNDKE
                     YKIFGDSHIDEIAEEHNLKVLAKLPIDPRLSAACDKGMIELFEGDWMEPVGKILENME
                     DKK"
     gene            23775..24134
                     /locus_tag="PKI50_03480"
     CDS             23775..24134
                     /locus_tag="PKI50_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT02805.1"
                     /translation="MIKIAVASENDMVTEHFGHCMNFNIFEAENDRIVKSESILNPGH
                     RPGFLPNFLNNMGVNVIISGGMGGGAIDIFNEKGIEVVLGARGYAKEAAEAYLKGTLK
                     STGSVCHEHQHHDECGK"
     gene            24151..24624
                     /locus_tag="PKI50_03485"
     CDS             24151..24624
                     /locus_tag="PKI50_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT02806.1"
                     /translation="MTASLYVIAILALSVSLLKSKERTILALKKAWKSFENILPQFLS
                     ILIIIGILLAVLSPEQISKLLGRESGWYGVLIAAVIGSVTLVPGFIAFPLAAALLKSG
                     AGYMQIAAFISALMMVGIVTIPVEVEYFGKRAAAVRNTAAFVFSLIVALVMGVVM"
     gene            24687..25166
                     /locus_tag="PKI50_03490"
     CDS             24687..25166
                     /locus_tag="PKI50_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT02807.1"
                     /translation="MAVLIFYPAVGSKSLQLTWSNLLEMMAILPPIFILLGLLDVWVK
                     RETMIKYMGDDSGIIGVLLAFFLGSAAAGPLYAAFPVAGVLLKKGSKLSNVFIMLGAW
                     STTKIPLILFEASSLGLKFMLVRMVMDLIGIAVIAYFTERMLSKGEIEAIYQNASNR"
     gene            25265..26317
                     /gene="fni"
                     /locus_tag="PKI50_03495"
     CDS             25265..26317
                     /gene="fni"
                     /locus_tag="PKI50_03495"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNT02808.1"
                     /translation="MEDQKSAREKRKDEHLKYFLLSKPSGYNGFSDVVLQNNSLPDAD
                     FDEISTECVFLNKYIDSPLMINAMTGGTEYSGDINRKLARLAVKFNIPIAVGSQTVAF
                     DNPKAADTFKRVRDIHRDGIVIANLGANRDIEDVYKAIDMIDADAVQLHINIVQEMCM
                     AEGDRNFRGVLENIKKIAALAEVPVIVKETGFGISFETAGKLQAAGVKYIDIGGKGGT
                     DFVSIESLRNSDRECPFLENWGIPTALSLFQCRKASSDMFIICSGGISKAEEVVKALC
                     MDGDMAALGGTILRILLEQGYDAAEKYMAALLYEIRVLMLLLGAKDITDLKNVSYLLK
                     GELGELVLSLKKLYKF"
     gene            complement(26282..27175)
                     /locus_tag="PKI50_03500"
     CDS             complement(26282..27175)
                     /locus_tag="PKI50_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT02809.1"
                     /translation="MDINFELYKVFYYVSKSLSFSEASEELFITQSAVSQSIKLLEEK
                     LKCKLFFRNTKRVKLTREGEILYSHIEQAFNFIKSGERILEERNSLTQGEIRIGTSDT
                     ICKYYLMPFIKSFNQAYPNIKIHLTNRTSPRCIELLRKGSVDLSVINIPEKLNYSNII
                     VKNTKRVRDVFIANENFKELKGRKVQLKELEEYPLLVLERNSTTREFFDSLMAKHKVS
                     IKPEIELGSIDILMEMSKIGLGIAFVPDECVRIGLAKDDVFIIDIKEEIPERNLGFVI
                     HSNIPLPVAASKFIELLEGEH"
     gene            27360..31133
                     /locus_tag="PKI50_03505"
     CDS             27360..31133
                     /locus_tag="PKI50_03505"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNT02810.1"
                     /translation="MNGNVRRIFVEKKDDYAVSAKDLFNDIRENLGIKSLTNLRIVNR
                     YDISGITDSEYNSAKNTIFSEPPVDNSYDEKIIIDKNDRLFAIEYLPGQFDQRADSAA
                     QCLQILTQKERPKVVTAKLIILSGEISDDEFEKIKNYCINPLELREASLEKPESLEAV
                     YPEPGDIKEVKDFITMDEAGLARLMEVMGFAMSSEDLRFCREYFKTSEKRNPTVTELR
                     VIDTYWSDHCRHTTFNTLIEKIEIEKGKYTEAIEKALLRYMDARAFVYGDSERDITLM
                     DMAVMGMKEMRKKGLLDDLEVSDEINACSIIVNADIDGKREEWLVMFKNETHNHPTEI
                     EPFGGAATCLGGAIRDPLSGRSYVYGAMRVTGSADPRASIEETLPGKLPQRKITTGAA
                     AGYSSYGNQIGLATGQVAEVYDEDFVAKRMEIGAVIGAAPKSNVIRKAPSEGDVIILL
                     GGRTGRDGCGGATGSSKEHTEESIQSCGAEVQKGNAPTERKIQRLFRDPMVSRMIKKC
                     NDFGAGGVSVAIGELADGLLIDLDAVPKKYEGLDGTELAISESQERMAVLLAPEDAES
                     FINYAAAENLEAVVVAKVTGDNRLRMQWRGQTIVDISRDFLNTNGVRQSTRVLIKSPD
                     AESCFNKLQAWEKSKEDLKGKWLENLKDLNVCSQKGLVERFDSTIGAGTILMPFGGIY
                     QETPSEGLAMKLPVLSGETNTATVMTYGYNPKLAKWSPFHGAVYAVVEAVAKVAALGG
                     DYGRIRLTLQEYFEKLGKVPEKWGKPAGALLGAFYAQMMLGVPAIGGKDSMSGTFKDL
                     NVPPTLVAFAVDTAEAGRIVSQEFKKSGSKVILLRAERDDKDLPDFEKLHKIFSKVHE
                     LIQKGLVLSSATVRIGGIAAAVSKMCFGNKLGFAFEKGYEERDLFTPEYGSIILEMDQ
                     SCDLNGVFSGMDYEIIGVTESTGYISIKGIKIELEEALKAWKAPLERIFPTHSGDNDD
                     KPQNIYYNSTNIVKSSAKIARPKVFIPVFPGTNCEYDTARAFEKAGGEPSIFVFRNIG
                     PSDIEASINEMVRLIGNTQIIALPGGFSSGDEPDGSGKFIATVFRNPRVKEAVMKFLR
                     EKDGLMLGICNGFQVLIKLGLLPYGEIMDMDESSPTLTFNKIGRHVSCMVRTKVVSSL
                     SPWLSKSKVGDIHTIAVSHGEGRFAASREIMDSLIKNGQVATQYVDLNGNPTYDSAFN
                     PNGSIEAVEGITSPDGRIFGKMGHSERTGANVGKNVPRDKEQLIFKAGVEYFS"
     gene            31161..31670
                     /gene="purE"
                     /locus_tag="PKI50_03510"
     CDS             31161..31670
                     /gene="purE"
                     /locus_tag="PKI50_03510"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNT02811.1"
                     /translation="MLVSSDKPKAAVIMGSDSDLSVVRKAIEILKAFEVDYEVNVISA
                     HRTPDRAAEYAKKAEENGFEVIIAAAGKAAHLPGVLAAFTTLPVIGVPIKSSVLDGLD
                     SLLSIVQMPSGIPVATVAVDGAENAGLLAVQILSVKYGNLKDKLKAYKEKMKQDVIKK
                     DEKIKEEVF"
     gene            31670..32374
                     /locus_tag="PKI50_03515"
     CDS             31670..32374
                     /locus_tag="PKI50_03515"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNT02812.1"
                     /translation="MKRLGMIYEGKAKKVYKTDDEALYIVEYKDDATAFNGLKKGTII
                     NKGVMNNRISEKLFRLLEEKGIDTHQVERISDREVVVKKVKIIPLEVLARNYAAGSLS
                     KRLGLEEGVKLNSTVLEFSYKNDELGDPMVNDYHIKAIGIATEQEVAKITEMALKINE
                     ILSEFMLTKGILLVDFKLEFGKTPDGKIILADEISPDTCRLWDAVTLEKMDKDRFRRD
                     MGKVEEGYMEVVKRLC"
     gene            32385..33785
                     /gene="purF"
                     /locus_tag="PKI50_03520"
     CDS             32385..33785
                     /gene="purF"
                     /locus_tag="PKI50_03520"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT02813.1"
                     /translation="MDKLKEECGIIGIYDKGNMEIESLTYYGLIALQHRGQESAGIAV
                     NRDGVITCYKEMGLVSEVFDDKILNLLKGDISIGHVRYSTTGDSFAHNAQPLLVKYKK
                     GSIALAHNGNLVNAGSVREELEDQGMIFTTTIDSEVIAGLIARSCSKGIEDAILDTMD
                     IIRGSYSLVIMTDDKLIGVRDSHGLRPLVIGRIGNGYVLASETCALDTIGASFIRDVR
                     PGEIIIIDENGMRSIQCRSTGKQAGCIFEYIYFARPDSVMDGISVYQAREKAGRILAE
                     EQPADADVVISVPDSGTPAAIGYSLQSGIPYAIGLIKNRYIGRTFIQPSQVTREIGVA
                     LKLNPLKANIEGKRVVMVDDSIVRGTTSRKIVQALKAAGAKEVHVRVSSPVITHSCHF
                     GIETSNRDELVGATSVLEAIREKIGADSLGYLSYNGILRSVGSTGEGFCTACFSGRYP
                     MDIPENCSKDVFERKK"
     gene            33811..34851
                     /gene="purM"
                     /locus_tag="PKI50_03525"
     CDS             33811..34851
                     /gene="purM"
                     /locus_tag="PKI50_03525"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNT02814.1"
                     /translation="MDEKLTYKAAGVDVEAGYEAVKLMKQHTKRTMIPGVLGELGSFG
                     GFFEIDRESYKNPVLVSGTDGVGTKLQIAFMTGKHDTIGIDCVAMCVNDVACHGAKPL
                     FFLDYIGTGKLMPNIAADIVKGVCDGCVEAGCALIGGETAEMPGFYKDGEYDLAGFAV
                     GIVDKGNVINGSNVKEGDVLIGIASSGIHSNGYSLVRKIFFGINNFDINTRFDELEGT
                     LGEELLKPTKIYVRTVQSLIVKHRINGIAHITGGGFIENIPRTLPAGLKAQIDLGSWY
                     IPPVFRLMQRLGSIEEKEMFNTFNMGIGLVLAVGKQDAEGILGTLEELGEKACIIGSV
                     AGGEGGIEFCGR"
     gene            34839..35456
                     /gene="purN"
                     /locus_tag="PKI50_03530"
     CDS             34839..35456
                     /gene="purN"
                     /locus_tag="PKI50_03530"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNT02815.1"
                     /translation="MRKVRIAVLVSGGGTNLQTLIDAVDKGDINGEIAAVISDNENAY
                     ALERARKHGIKAIYINRKLLAERLMAELQKLDIELVVLAGFLSILDRELVKAYEGRII
                     NIHPSLIPSFCGKGFYGERVHKAALEYGVKVSGATVHFVDEGTDSGPIIFQEAVPVYF
                     EDTSETLAARVLQVEHRLLPAAVGLFCEGRLRIEGRKVKILKEEV"
     gene            35464..37005
                     /gene="purH"
                     /locus_tag="PKI50_03535"
     CDS             35464..37005
                     /gene="purH"
                     /locus_tag="PKI50_03535"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNT02816.1"
                     /translation="MIKRALISVSDKTGIVDLARELAKQGVEIISTGGTAKALEEAGI
                     KVIGISDITGYPECLDGRVKTLHPNIHGGLLAIRDKAEHMDKLRELGIGTIDMVVVNL
                     YPFRKTIEKEGTELPEAIENIDIGGPSMLRAAAKNYKYVTVVVDPKDYSKVLSEINNS
                     GDTTEATRFELAAKVFSHTASYDALIGNYLWSKAGLPKYPETVTMTFEKVQDLRYGEN
                     PHQGAAYYKEMKKVSGSIAAAVQLHGKELSFNNINDANAALETLKEFDEPAVVALKHA
                     NPCGVGVGSSIYEAYMKAYEGDPVSIFGGIVALNREVDEKTASEIGKIFVEIVIAPSY
                     SEEAMNVLTGKKNIRIMKLEDISRKPEADIDVKKVGGGLLIQDADWADFNMADINYIT
                     DRKPTEKEMQDMLFAWKVVKHVKSNAIVVAKDGMTLGIGPGQTNRIWSANMSLERSGE
                     KAKGAIIASDAFFPFGDVVESAAKAGITAIIQPGGSLRDQESIDGCNKHGIAMIFTGV
                     RHFKH"
     gene            37019..38281
                     /gene="purD"
                     /locus_tag="PKI50_03540"
     CDS             37019..38281
                     /gene="purD"
                     /locus_tag="PKI50_03540"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNT02817.1"
                     /translation="MKVLVVGSGGREHTLVWKIAQSKLADKVYCAPGNGGISELAECV
                     DIKADDIDGLLEFAKTEGIGLTVVGPEVPLSLGIVDCFEENGMRIFGPCRKGAMLESS
                     KAFSKDFMIRNKIPTAAYKVYYEAEKAKAGLDDFGFPVVIKADGLAAGKGVIIAQNRE
                     EAISAIDDMLMVRKFGEAGSKIVIEEFLVGKEASILAFVDGKTAVPMVSAQDYKRALD
                     NDEGLNTGGMGAVSPAFYYDEKTAAVVERDIIRRTVDALKAEGICYKGVLYFGIMITE
                     KGPKVLEYNARFGDPETEVVLLRLESDLVEIMNSVIDEKLEAQKIQWKDEQAVCVVMA
                     SGGYPEHYEKGYEIKGLDEAAEIDGVTVFHAGTKKASGKTVTAGGRVLVISAAAKNYE
                     EARDKAYSAISSIAFEKAHYRKDIGKKD"
     gene            38735..39505
                     /locus_tag="PKI50_03545"
     CDS             38735..39505
                     /locus_tag="PKI50_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT02818.1"
                     /translation="MIRKIVKIDEDKCNGCGLCVPNCAEGAIKIIDGKARLIADNLCD
                     GLGACLGECPRGAITITEREADEFDETAVHEHLDRKEAKPEVKQEAHSCSGHAHIGGG
                     CPGSMAREIKLRENPAEAAAVTSGDIEVKIKSQLTQWPVQLMLAPQKAPYFENADLLI
                     TADCVPFAYPNYHLELLKGKKVVVGCPKLDDIGYYMEKLTGIIRNNDIKSVTVTFMEV
                     PCCGGIVRAAETALEMSGKNIPLHKIRIKINGEAEKIS"
     regulatory      39618..39811
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            39942..43286
                     /locus_tag="PKI50_03550"
     CDS             39942..43286
                     /locus_tag="PKI50_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HNT02819.1"
                     /translation="MGKAYSYRRVSAIMLIMIFLTVIAPVDLYGTDYTGVLDDVVNYY
                     KSDKPGLDSWWELVALKGTGQNLSDDSWILPQWESEDLPAAAANPADYSGFILGLMAM
                     DENPQNALGGRNLTEELAGRQEEGGAFGNGAINMHIWSMVALDAAEENYDRESAVEWL
                     LSNQKADGGFALSGINGDPDMTGMALLALSPYINQADVSSAVSQAVEYLENAQCESGG
                     YSSWGIENSNSAAMVISGLVSAGQGVSSSILDALLEYRLPDNTFSYQKGGATNFLSTA
                     QSLIALGDIIENKSIFQRLSISQQPTDGEKVRIRVEGSSYSMVNKEITVTSAVYAVDA
                     LKQVLDSENITYDIQDGVWGQYINGIDGETAGKFGGYDGWLYLVNGEMASVGVGEYKI
                     EDGDELVFYYGMYPPDTLIPQVDIQPVNPKVGDNLTITVTSSYYDWNTGSTVELKVQN
                     AEISFNGKTYATDANGQAIVPSPADKGTYTLKVSKDNDGSYPSIVRIPGINVIYRANG
                     NGGGGSGSGGSGSGSGGSAETATLSVEGDSRKGTILPAVQVLLRDGDTAYSILARKMG
                     SKVKAKGSGSNIYVYEIDGLAEFDRGPSSGWMFEVNGRNPDISASAYVLKDKDVVNWY
                     YSSDSNESLGATGQTGTAQSGNSSAEISSTSNEAIKKALSENIQNSANKILTQENISD
                     WSVFALQRAGRSIPEKYIESLKVYINEHAAGSGSATDIARMVLAASAAGMNPANIDGN
                     NLIEKLFSMEKIDRQGVTGPVFALIALDSGPYSIPSGARWTRDKLVSLILEYQNEDGG
                     FCLAKDEKSDIDLTAMALQSLSGYSSESAASSVEKAIDWLSNLKPEEEQTSESISQII
                     IALTSLGKDPGSIQFGMYDGDLLTCLLKYKKEDGGFSHTLDGDSDEMATEQALMAITS
                     YSMYVNGSGKLYSLQVPNGAYSDLHAISEWALPYVAKASEQGLMEGIKTDASGQRFEP
                     RRNISRAELTALLLRILGEEPSKSFTPVYNDIKEGSWYSGYVLRAREKGIINGVTEVT
                     FDPNKPVTRQEMAVMTARAFNLGEKASGSRINDLDIASEWARQAIEAVYDNGIMMGES
                     NMFNPKGYVSREMAAAVGVRLYNMKQ"
     gene            43357..43959
                     /locus_tag="PKI50_03555"
     CDS             43357..43959
                     /locus_tag="PKI50_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HNT02820.1"
                     /translation="MRRFTFLLYAVLLIILSGCGNAADADIPNSEKISKPVLSDESNC
                     IKGTKKEDIPDGKTSIKPESKSESKNNEEHVVNQEAATAKVHISITGHKDVGIILADT
                     AVNIDEGDTVIDVLKRTAELNSIQLECSGIKAMAYVKGIGNTYEFDYGPKSGWIYRVN
                     GEISNVGAGKYEVKDGDYIEWLYTVDLGREFDTESGGAQE"
     gene            43956..44834
                     /locus_tag="PKI50_03560"
     CDS             43956..44834
                     /locus_tag="PKI50_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNT02821.1"
                     /translation="MNAGFSSVHPGVCFTYYTVIMAFAMILYHPAYLASMPIALITWL
                     LLHEKGRSLSGSWHYYLLTGIAVLIINPLINHRGERVLFFLGEQAVTFEAVVYGFTMM
                     LSMLTILIAFICFRQAVDNHKFLYLFSSILPKTAFLIMMSMGFVPLLTDRMKQISIVQ
                     KARGLDMTEGTAVKRAKDGMQILKILISWSLEGALQTADSMKGRGYGIAKRSSYMKYR
                     IDGRDWLIMASIAILSAITSIGLYFGYGKLVIFPSLEIISMDITGIVFYSAFCLLLLI
                     PIIVEGREILIWHCYK"
     gene            44816..46480
                     /locus_tag="PKI50_03565"
     CDS             44816..46480
                     /locus_tag="PKI50_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNT02822.1"
                     /translation="MALLQINNFNFTYAGDTKKVLSGINLTVGQGEFIVLCGPSGSGK
                     TTLLRNIKREISPAGIKTGDILYKGMALDELKAEVSAKEIGMVFQEPDSQIVMDTVIH
                     ELAFSLENFGYAPDFINKKLSEMAAFFGLTEYLHKPVQELSGGQKQVINICSVMLTEP
                     KLLLLDEPTSQLDPVAARELISLIHQINREFSVTVIISEHRLEELFSIADRVVYMDGS
                     IIKYCNSPDGVSKEIMEAKDEYAVKSIPPIVRLFFSLSKEKGKGPGCGIPMSVREGKQ
                     FMQNIRCSVNGLTDMEIKKDWVEEIINCKDIGFRYAREEPMVLEKLTLKIMKGEFMAI
                     LGGNGSGKSTLLLAMAGLITPQQGSICVRKKNITYMKQSERNGIIGYVAQNPLLHFTL
                     DTVKEELEFALQNSLKTGSKAKMEELIELFGLKDLTDRHPYDLSGGQQQNLAVACALI
                     HEPEILLIDEPTKGLDSISKEKLAELLKSLKGKGTSVVMATHDIEFAAHYAEKCAMLF
                     DGSISDCAEPSRFFSGNYFYTTSINRAVRDYLPHAITYEDVIRACL"
     gene            46471..47031
                     /locus_tag="PKI50_03570"
     CDS             46471..47031
                     /locus_tag="PKI50_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNT02823.1"
                     /translation="MPMIRFETKKQDPRELVLISMLAAIAAISRILFAPLPNFKPMSF
                     VIIVTGIVFGPESGFMVGSVSAILSNIFFGQGPWTLLQMLAWGLMGLTAGLLRNTVWI
                     KSRYGILMFGFSWGFIFGWIMDAWYIIGFIRPLSLMTIIGGLSASFYFDLMHALANTF
                     FLGIFSKSWIKLFNRIKIKYGLLEDS"
     gene            complement(47058..47477)
                     /locus_tag="PKI50_03575"
     CDS             complement(47058..47477)
                     /locus_tag="PKI50_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02824.1"
                     /translation="MFRTNLKKRIGPLLVMLIAVMMIALGLLRISLGFAGEKDTAVIT
                     GIRRQGGERGEAIPNRYTYVVNYAFTLPDGEKIDGFTYDIRDAVYIKVSDSNIRMTPI
                     RYFKALPHINALESDSGLKAGNLILIGIGIFMIKFVK"
     gene            complement(47482..48054)
                     /locus_tag="PKI50_03580"
     CDS             complement(47482..48054)
                     /locus_tag="PKI50_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02825.1"
                     /translation="MDEYKQTKFYWEIDVPIFKNNAILKDLGIALGIPFGLLILILFI
                     ASGGDVSADGIKYPLTLIGLLFIAGFLFIMVVYGGRYSAGYLIDKNGILNYTQRKQAK
                     KNLIVNSLLIIIGLFSKRPSAAGAGLLAQTRQSVFIAWKSIRKVKVYPKSRSIVVIGG
                     FAEKIALFCNEDNFHLVSDIILSKFNANGE"
     gene            complement(48142..48633)
                     /locus_tag="PKI50_03585"
     CDS             complement(48142..48633)
                     /locus_tag="PKI50_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02826.1"
                     /translation="MNTIKRKLMIYMMLSSFGILISFTLFAGYFHIVVVFSILTTILL
                     LVFSLNEYRRYAFARLIIENKIIHIEAAETESESKMNKSIEIFVSCFGILLDSKIIKF
                     NTEGISLKKVEVGHDYILLAYGKGEKYKTIRLLHSNINEPELRDIIEKFRRETGIIPE
                     LAG"
     gene            complement(48707..50455)
                     /locus_tag="PKI50_03590"
     CDS             complement(48707..50455)
                     /locus_tag="PKI50_03590"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNT02827.1"
                     /translation="MKKFLSIVTVLALLFSMAAGFTTVVSAAEEGYAWVLVDTHEYPV
                     PEADSFYGSLKGNTIELQYAWAYGLGIYASKDYDNPTDMHAIYTWSNPPSTIMPNQTV
                     TLKLEQNVISNRNGNYSIGFNPYFHMDMEDIDIGYATASKVIAKITYADGTTAKYPGI
                     GYNQDAQAQQSFTADMTLNFIGEGRPGDKHSLYVGVYAGSPGSVGVRYTYEWKQTSSA
                     NAAPISSGTTDLGTYSWAGEWDSNWGKMLITQNGASVTGTYTHDSGKINGTISGNVFT
                     GTWSESPSYSPPSDAGDMELTMSADGKSFTGRWRYGSEGSWSNWDGSMRITEVIPAPK
                     PAATSPAPVITTTQANSAGSNAETFESGSRIMWQPADGLGYRLFRSTSKSDLGISVTD
                     FYITGTSYADVNIEANTTYYYTVKPVLAEAKPFEGIDEKLGAAIATYTVTTGSQVYKP
                     GSFKHFIMLKLDNPNMSVDGVIQEVDPGRSTSPMTIAGRTMVPIRAVVEAMGGTIGWE
                     QDTRKITLTARGNVVEMWLDKTDIKINGVSQKMDVAPVSKNGRTFVPVRFAAENLNCK
                     VDWINSTKEAVIVYEE"
     gene            complement(50624..52024)
                     /locus_tag="PKI50_03595"
     CDS             complement(50624..52024)
                     /locus_tag="PKI50_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT02828.1"
                     /translation="MLRDERSKTIIAFSSFSSWLLSFLFGGQVLLTIASINNVNVNEL
                     TFGAIAAHFIGLFACGFFIKSTKAAKGLMLFSIPVCILGTVVFFFIPSIMWHISLLIL
                     SFLSGACVAAWGFYFKMLAFSYERIKIIADVLIYSNILMVIINTAAVLISSYLGLALS
                     ILMLSISLFFHTKLAVDQNTAEERHTRQPIKAVSPIKPLAFLCLFIVIITINSGLMYQ
                     VINPAFAHHEWLVSWYWALPYIAALYIVRNLSGKANRSYILYVAIAMIGLSSVGFMIL
                     DRSAASYLIIDTLMLGACGVYDLFWWSILGEMLDYHHNPAKILGIGLSANVFGVLIGG
                     MFGSTIADSDTGSIDSSVLALVVVFIVLILLPLLHRHLSGLLKNHIFLTALSEIEPSK
                     QKKAIENFTVIGRLTERESEIAALLLKGRTYKMIADELCLSENTVKTHIKNIYSKFNI
                     QSKTELIKLLTGKETH"
     gene            complement(52170..52352)
                     /locus_tag="PKI50_03600"
     CDS             complement(52170..52352)
                     /locus_tag="PKI50_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02829.1"
                     /translation="MNIIDETYNGEKYPIIERKYNSYWFPENYNPDNEEAQAEELVNS
                     YMNHEEIINNKNKNSK"
     gene            complement(52349..52534)
                     /locus_tag="PKI50_03605"
     CDS             complement(52349..52534)
                     /locus_tag="PKI50_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02830.1"
                     /translation="MSTKINKKLNVLTPAAEEPITDYEIVMAKINHDSHKKYNGIENI
                     ALENEDIKEKDKDEVKP"
     gene            52686..54611
                     /locus_tag="PKI50_03610"
     CDS             52686..54611
                     /locus_tag="PKI50_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT02831.1"
                     /translation="MNYLENLNKEQREAVLHTEGPLLILAGAGSGKTRVLTYRIAYLI
                     DEKGIYPSNILAITFTNKAAREMKERVHRLIGNTDNMWISTFHSACVRMLRKNVESLK
                     GYKKNFVIYDTKDQETLIKECLKELNLNEKNFPFRVVSAEISAAKDRLIPPREFYDKN
                     MHDIRRRKLADIYMLYQKKLEKNNALDFDDIISKTVELLKLNPDILEYYQNKFRYILV
                     DEYQDTNFSQYTFIRLLAAQHRNICVVGDDDQGIYSFRGADIGNILNFEKDFPGAKVV
                     KLEQNYRSTETILEAANAVISNNYGRKSKKLWTDKGTGRSIIYHYAVDEREEANFIMD
                     EVERMIAQENRKLGEFAILYRTNAQSRVLEETCMSHGLPYRIVGGFKFYDRKEVKDII
                     AYLRVIQNPDEDLSLKRIINVPKRGIGNSTLEAIAQYARTTGDSIFGALLEVDRIEGV
                     STKAKKGIKEFVKIMSDLMGIAETEMVSIIVKEVLERTGYLDQLEKDGDEESLMRAEN
                     IKELLSATFEFEELNESALLPEFLEQMALMSDIDAVEEGKDALIMMTLHSAKGLEFPF
                     VFISGMEEGVFPSQRSYFEDNQMEEERRLMYVGITRAKERLYLTAAFERTLYGNTTFN
                     TISKFVREIPKELLVKV"
     gene            54863..56863
                     /gene="ligA"
                     /locus_tag="PKI50_03615"
     CDS             54863..56863
                     /gene="ligA"
                     /locus_tag="PKI50_03615"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNT02832.1"
                     /translation="MGIDKLEKRADELIKLINRHNYNYYVLDKPEISDYEYDLLMKEL
                     IELENTFPALKRADSPTQRVGGEVLKGFAEVVHKTPKLSLSNVFDENDIRDFDYRVRK
                     AVGGDVEYVVELKIDGLTVVLNYEEGRFVLGATRGDGIKGEDITSNLKTVKSIPLLLE
                     EEVDLEVRGEVFMSKKAFEELNARREEQEEPLFANPRNASAGSLRQLDTKVTAGRPLD
                     IFIFNLESIEGKNFDTHVETLEYLKELGFKVSPFISVHKSSEEIIEKCKYWADKRGEL
                     PFVIDGLVIKVNNLRQREAMGYTTKNPRWATAYKFPPETKKTKVKDIIVQVGRTGAIT
                     PTAELEPVRLAGSVISRATLHNEDYIKEKDIRVGDTVLIKKAGDVIPEVVEVVREDRD
                     GSQQPFEMPKKCPVCQSDTIREQGEAVTKCTNISCPAQLKRALFHFVSRDAMNIEGFG
                     PQIMSLLMDKDFIRDAADIYLLKNHRDELVEIERMGEKSVQNLLDAIEASKVNPLNRL
                     IYALGIRMIGQRASQVLANEFEDIEAMFDADYERLTGISEIGDKMAESIITFFKQEQN
                     IRLIRKLQECGVNTKGSKKEIKENEHFKGKTFVLTGGLANFTREQAKEIIESFGGKVS
                     GSVSNKTDYVLAGENAGSKLDKANELNVAVIDEDTFRSWAEI"
     gene            57299..58954
                     /locus_tag="PKI50_03620"
     CDS             57299..58954
                     /locus_tag="PKI50_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT02833.1"
                     /translation="MKKSLVLVLAMLMILSFALSACAPKAPAETPGAETPAPAAEPKI
                     FRTNNMSEPGSLDPALAQGTHESWILDHAFEGLMARDPSGISVPGMAESYTLADDEVT
                     YTFKLRDGITWSNGDPVTAYDFEFAWKRALDPDLAADYAFQLYYLKGGEAYNTGAGSV
                     NDVGVKAIDDKTLEVVLATPTAYFLELTSFYTYYPVNKKVVEANADWAKSADTHVSNG
                     PFKLTGWEHNATITLAKSENYYNKEAIKLDGIELAIIADENTAWQKYLGGEFDFLTPL
                     PQAVTAQLKAEGNPELVIGGEVGTYYYNLNNKLKPFDNAKIRKALSLTLDRQTICDKI
                     AQGGQLPAEGVVPFGMLDENNKEYRDGAGKLVEYNVEEGKKLFAEGLAETGFKVEDFS
                     KFTLLYNTSEAHKIIAQAAQEMWRTNLGIELTLENVDFQVKLDREKAGDFQISRAGWI
                     GDYIDPMTFMDLWLSEGAFNDAKYNNPEYDRLIKESKATTDQKVRMENMRKAEQMLME
                     AMPVVPVYFYTQPYAQKPYVTGVFKPLNRYPIFTFADMDMSKR"
     gene            59054..59983
                     /locus_tag="PKI50_03625"
     CDS             59054..59983
                     /locus_tag="PKI50_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02834.1"
                     /translation="MKKYLIKRVLMSVVTIWAIITITFVLMHSVPGNPFRQENKMPKV
                     VYENLLKKYGLDKPLSEQYFIYLGKIVKGDFGDSMKSRVETVNDMIRRGFPVSAVIGA
                     EALLIAIIFGPALGAIAALNQNRWPDYLSMIIAIIGISVPSFIMGTVFIQFLARNIEW
                     LPIGGWGSFRHSILPSLALALMPLAQLARFMRSSMLEVMGQDYINTAKSKGISKATVV
                     LRHAVRNAILPVVSVLGTVATNLLVGSFVIEKIFGIPGLGMFFIISINNRDYPLIMGT
                     TIFYAIILIVMLLIVDIAYMFIDPRIKLAKEGR"
     gene            59980..60927
                     /locus_tag="PKI50_03630"
     CDS             59980..60927
                     /locus_tag="PKI50_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02835.1"
                     /translation="MMITEFEYSPELFELASKEDRNAEKIRRPSIKYWPDVWRRLKLN
                     KLAMVGLSLIVFFGIMAFVGTQINGYTYFEQNYSLINVHPDSVHWFGTDELGRDLFTR
                     TWFGVRYSLFIGVAAAFIDFSIGVLYGGIAGITTRRIDAVMMRIAEVIYSIPYLLVVI
                     LISVVFSAAGQGSSMFVLILALSLTSWVPMAILVRGQVLQLKESEYSLASESLGADKK
                     WILRKHIIPNTLGPILVNITLSIPRAIFAEATLGFIGLGLQAPKSSLGTLANSGLAGL
                     PVGNSYQLFIPAIFISLIMFAFNVLGDGLRDALDPRLRK"
     gene            60942..61952
                     /locus_tag="PKI50_03635"
     CDS             60942..61952
                     /locus_tag="PKI50_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02836.1"
                     /translation="MENVLEVKNLKISFQTFFGEVEAVRGITFSVGKKETLAIVGESG
                     CGKSVTANGIMQLLPKPPAFFKGGEILFNGEDLLKKTEKQMQAIRGNKISMVFQDPMT
                     SLNPTMKIGYQIVEGLVKHNKMSREEAKKKSIEMLELVAVPQPEKRINQYPHEFSGGM
                     RQRVMIALAMVCNPQLLIADEPTTALDVTVQAQILELMKKLQDQMGMSIILITHDLGI
                     VADMSDRVVVMYAGQILEVGLTDEIFAAPKHPYTRKLLASVPRLDMSREEPLYSIDGT
                     PPDLYVPPKGCSFYDRCSEAMKICKNNMPEFDEHSATHQSRCWLNHPMAQKTNSTDGR
                     EV"
     gene            61949..62905
                     /locus_tag="PKI50_03640"
     CDS             61949..62905
                     /locus_tag="PKI50_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT02837.1"
                     /translation="MMTEPLISARNIKKYFDVKGGKLKAVDGISFDIFPRETFGLVGE
                     SGCGKSTAGRTIVRLYDPTEGELIFNGQNIYKLSRKDMQEIRRDFQMIFQDPYASLNP
                     RMTVEDIVAEPLDIHQTYKNRAERTERVIELLRLVGLNDEHAMRFPHEFSGGQRQRIG
                     IARALALNPKFIVCDEPISALDVSIQAQVVNLLKELQDGLGLTYMFIAHDLSMVRYIS
                     DRVAVMYLGHMMELAESDELYSNPVHPYTKALLSAIPIPNPEIQRNKKRIMLEGDVPS
                     PINPREGCRFVDRCQYAIGRCREETPVFEEKAANHFAACHVV"
     gene            63035..63703
                     /locus_tag="PKI50_03645"
     CDS             63035..63703
                     /locus_tag="PKI50_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02838.1"
                     /translation="MTLEDYLEEGCVYHVVSIIDLKKTLHEGIRYDDKNTYACKYTDF
                     HSFFDLYKPKGIPDWVERRKAIFASIGFKEGHKWHSHSAILRIRILKDRCWICNENLA
                     NFIYEPFILQHMDGFEGTKEFMKANGKAFAGKYWQNSLSFNDNLIKRYDKREGYDAEV
                     LVMHPIPPRNIECLYIVSDHRIMSYQAWKEYFTPGSPHAEVQYNRYLQPDNMSSAWKY
                     PGSQ"
     gene            complement(63637..65004)
                     /locus_tag="PKI50_03650"
     CDS             complement(63637..65004)
                     /locus_tag="PKI50_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02839.1"
                     /translation="MIFEFSYPDIIKQCNTMTYLNVISFCLLSGCGSIEMPDEQELSH
                     IYKLLKGAGSIRMPKNITFMSSLYKRCIPIYAAIPEASYDFKSFRWLEKKMKKSVEPN
                     VLAHSICSMTALIPIIQNGEIQMENKEFIAYCLGVNSIKQARFLIDYLKLGDFYYSGE
                     DTGDNVYGEYRITINNENPDIKTQFFVAEALSSILGLIDRDDSYSRDLVPRLKKALEV
                     LPVLCENITENINDISSRDLSVVGLCLLSILGHTAVYKETVYSTANVIGMELCERLAH
                     TGDIARNISDDSFSSFITLCNCMNFLIKLHEVNDLIVYEASFLKLYDRIDSYWDRSSG
                     LFLVTRKNKQKYSFKETAAVFAALCMLRSCLTDAGLFMHVDRQLSAFYSATFISSKLF
                     NNQFYPILQDTKLELHNLGSTEKRSAPVFSEYFEVKLNKRKYHCEPGYFQAEDILSGC
                     KYLLY"
     gene            65193..66173
                     /locus_tag="PKI50_03655"
     CDS             65193..66173
                     /locus_tag="PKI50_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HNT02840.1"
                     /translation="MKEGKIPPELLKSLVFNNIKVRNEEVILRPEIGEDCAAIDFGSH
                     ACVLSTDPITGAVKNAGALAIHISCNDVAASGVRPVGIMITILAPPEAAEGDIRRVME
                     EAGEAASKLGVEIIGGHTEVTSAVNRIVISTTALGKVRKDRIVRSSGAKAGDDVIMTK
                     WAGLEGTSIIASDKGKELEAVLTPEEIESARSFIDNISVVEEGVTAGEFGANSMHDVT
                     EGGILGAAWEIAESSGKGIDIYIDKIPVWEVTKKICSFYGIDPYRLISSGSMLITASK
                     GAELAKLLEKKRIPAAIIGRITEAGRNIIRNGNISLLNPPDTDEIYKALK"
BASE COUNT        20634 a        11747 c        16282 g        17638 t
ORIGIN      
        1 tggaaaaagt tcatacccag gtaagctggt tatgaaccta gtatacgaac aatgccctca
       61 cgctgcggca cgtcgggcag tgtcatgggg cagacgtgcc ttgggttgca gaggatatgc
      121 taaactgtag ttagatttaa gtagcaacca cacctcttca ccgggtgtca ccaccgccag
      181 ggggcagggc tctgtgggtc cggttcaggg atgtcgtgag tgcgaacccg ttatgtgaca
      241 tatggcggat ttcatggagt agtagtatta tctctgctaa atacctagtg agatgctaaa
      301 atagaaagtc atactttctt ttgccggata tgatacgtct tatttcaact atatcgtcga
      361 acactacata gaagatcaag tgattgtcaa ttattagtat tctatagcct aagctttgaa
      421 gcctaatatc ttttgggatt acacctataa aagggaattg ttcaagacga gagattgatt
      481 catcaatttt atctaggaaa tttaatgcag attgagggct atcaattttt atataatcga
      541 ctatttccgc taagtccttt gcagaacttg gcaggtactc aattttatac ttcttttcca
      601 ttgatagtct cccttaattc tttcataaga tttttatgag agattctatt gtcggagtat
      661 ttgctctcag cttctgcgac agataacttt tggtataatt caataagtgc ctgctgtttt
      721 tcataaagag ccatactcat tacaacaagg ttaccttgac cattctttgt tataaataca
      781 ggttcggatt cagtatggca tatttcggat atatcattaa agttattgcg tagatcagaa
      841 attggtctaa taataggcat tataactcac ctcactaact ttttatcatt ataataatat
      901 tattatatca taataatgat aaaatatgag aaaaatttat atttttatat tttattcagt
      961 ttgccgctgg agcccgccat acatcatcta acaatgcgct cacgctgcgg cacggtgggc
     1021 aaggacaagg agaatgcgtg ccttgggttg caaagatatg caatactgta gtctgactta
     1081 agtagcaacc acacctcttc accgggaggg tcgctctgcc agggggagag gctctgtggg
     1141 tccggttcag tgatgttgtg agtgcggaac cgttagctga cagataatgc agattatgac
     1201 ttatatgaga agtactattc ttatatttcg tattattagt aatttgcgaa gtgaaaagat
     1261 ttagttttgc agtgcgataa tctgaatata ttatataaaa aggaggcgag tgaatgagtc
     1321 aaaataataa gagtttaaat aagataaaat gggctgcacg aattagcgct atggttataa
     1381 ttgcattcgg tttgccgttc tattttggat atggcaatcc actgcccttt attaaccctg
     1441 attacacttt atgggacaac atgtggctaa tgattttccc gattatgttt ataggacttg
     1501 cgcttggttt gaagttcgaa aaaactggcg gctatcttgt gatcatttcg gtagggctag
     1561 gactacttat gggattgttc atgcaaggag aattagtgtc tcatatgctt gtaccacttt
     1621 tggtaggtgt tctttactta attgttggat ataaaagcga taagaattat tgatgaatca
     1681 aactgttcac attatgtgta aacttctatg cagagtttgt gagagattta aacccaagag
     1741 aggtaatatt tatacaggcg acacatgtca gatcccgggt accgaaagcg gtatataaag
     1801 attattaagc gaggcgttgc ggggcatcat ccaccatcta acaatgcact cacgctgcgg
     1861 caggttgggc tggagtatta agtagcgtgc cttgggcgct gccacacccc ttcactggga
     1921 acaaatctcc gccaggggga aggtcactgc cggtccggtt cagggacgtc gtgagtgcga
     1981 acccgttttg tgaaatatct tgctttgaag tgccgcccaa gtgcgggtgt ttgaaaggaa
     2041 gcctttggat taagaattta taatccaatg aaaatactaa taacaagagg taaggttatg
     2101 aaaataatct caataatcag tagttctcgg aagaatggaa atacagaaaa tctagttagt
     2161 atattagaag aagaactaaa atcagtgtca gaattagaac atgaagaaat agaaattgaa
     2221 agaatatatc ttggacaggc taatcttaaa atgtgtatgg ggtgcaggat ctgctttgat
     2281 aaaggggaac aattttgtcc gttgaaggat gacttattaa ccatcagaga gaaggtggtt
     2341 gaagctgatg gtgttatact tgcaagcccg gtttatgttg aggatgttaa tggtattatg
     2401 aagaactgga tcgacaggat ggcattttat tgccataggc cagcattggc aggtaaggta
     2461 gctgtgacta taacgacatc gggagcagga tcaaccaatc atgcgttaaa gacaattagt
     2521 tttgcaatta tgtcatgggg agcatatctc ggcggacagt ataaatttcg tacaggcgct
     2581 ttgatgaaaa gagaagaaat atacgagcac aatcatagta aagttaatga agtagcaaga
     2641 aagctaattg atgatataaa gaatcagaaa gcctttatgc ctacgcttta ttcgcttatt
     2701 atatttaaag tacagcaaaa gctctggcaa aagaatacag actcacagaa tagtattgat
     2761 tatacatatt ggaacaataa agggtggctt gaaccaaagt gtgtatttta tactactatt
     2821 agaacaaatt ggttcaaagt aaaaattgca agattatttg gggcaataat agcgctgttt
     2881 ttcaaatagt tatgtattga agatcaggca agagctttat tggaccgcgg catccgtgtc
     2941 gctattgact ctggaggcgt ggtgctttat ataacaatgc cttcacgctt cggcgcgatg
     3001 ggcaggtgca agtagtattc gtgccttggg cattaccaca tcccttcacc gggagggtcg
     3061 ttccgccaag gggtaaggct ctgtaagacc agttcggatg tgtcgtgagt gcgggaccgt
     3121 tatgcgacag atggtgcaaa ttggcttaca taagaagtct gattgctata ttgtatgatg
     3181 gtaaaaaaaa gcgaaatgaa gattattaat gtaaaaggtt cttgacatta ataaccttta
     3241 tgatacaatg aatgtaaaga actttttaca tttggaggtg tactatgaaa tcacttttct
     3301 caaaattagg gtttcgtaaa atggatgaaa tggaaaagaa cattgcactt aaagcacaac
     3361 aaaatgcttt gatttatgtt ttattggcct tagcaatgtg gtctttctat gaatctttta
     3421 aagcgtattc acaacacaca tctatcaact caataccaag ttttttatta gtaaccacta
     3481 gtttggttca agttttctca caactcgtgt tacaaaaaag agttgtaaaa gatgatgatg
     3541 agtataaaga cgataatcca cttttgaaaa ttatcatagt aggtgtaatt ataagtgcga
     3601 ttattgtttc gattggcgct ttattaatcg tttcggggat tcaaaaatga agaataaaat
     3661 caaacaatta agaaaaaaca aaggtttacg acaagaagat atagctatgc tattaaatgt
     3721 ttcaaggcaa acaatagttg ctatagaaaa cgataaatac aatcctacac tggaattggc
     3781 tatgaaaatg gccaggttat tggatacgac agtggaagaa ttgtttatat tagagtaact
     3841 tattggcatg agcataatgt aaagttgagt taattcgcat tcttcttata atgttcaaat
     3901 tatatgcgga gtttgtatga tttaaactca agagagacaa tactaaataa gcaacacatt
     3961 taaggtttcg ggtaccggaa cggtatagaa aaaagagcaa ggctctgcgt gttcggttca
     4021 gcggtgtcgt gagtgcggaa acgttagacg aaatcttatt tgtgtatata agggggtggt
     4081 acattttgat taatgaaaga gaaaaagtta ttaaggctaa ggaagttttg attaagattg
     4141 caaatggaat aaatcctgtc gatggaacgc ctattaatga agaaagtttt ttaaacgatc
     4201 ctagaatcat aagatgtttt ttttacatat ccgaattact aaaccgggta atagctggac
     4261 aaacaaatca aaacttgcct aagccaacag agcttcatat cacaaaggaa gaaaaaagtt
     4321 tggttaagtt tcctgaaaat aagattgggg taaatgagtt tgcaaaatgt attaataatg
     4381 tgattgattt aaataaaagc aagaaattat cgggagtaga attaaacaaa cagttaaaga
     4441 aaatgggagt gttgagtgaa aatattgatg agcaaggtaa ggtaaggacg attattaata
     4501 ataaatcggt tgactatggt tttgaatcag aaaaaagaaa ctataatggc aatgaatatg
     4561 agatggtcgt aattaacgat attggcaagg cctacatatt agaaaattta gaagacatta
     4621 tgaagtatta ataatttact tactagagta tcagacttgc ctaacaatgt gttgccgact
     4681 tcgcttcgct acgatactaa tccccgggcc taagcgcatc gcgcccggct gacgccttaa
     4741 ggctcggtgg ctacggtagc acggaaccgt tatacgccat accatgctaa aattcaaaaa
     4801 gtagaaaaca tagaattatt ttaggatata aatcctttgt ttatacacct caagttatcg
     4861 aaatagataa gtaccctgat ataagggacg tagcagatct gccaatactt gtttcaccgg
     4921 acgccatatt agaaacgcaa caagtatgta gaatttaaag tgttgctgct gaagttattg
     4981 gctgcaagct aatagagaga agtcataatt aattagattt aatgcaatcg caatgggagg
     5041 taagagtatg ggaattactc catttaaatc ttctggcaga gggcctgcta tattgagaaa
     5101 ttgtatatta ggggtatggg ttttgatgtt tgcttttaag caaagcacga catttatggc
     5161 tagaaacagt ggtgaggtaa ccgagattgt tgcaggtttg ctaattatat ttttttcatt
     5221 gtatgatact tatgtaagaa gagaaaagaa tgatgtgtgg ggaataccaa catggattct
     5281 tggcgggttg attttgacaa ttggtatatt attttacctt tataagaacc catttgcttg
     5341 acaataaaat aatgcatata taattattac tttcacgaaa caatagcgat agccgtcata
     5401 ctgccaaaat atgtgaagca gagttgattt atatgtatac tatatgagaa atggagatga
     5461 gaaaaatgac tgttagaata gcaaaactgt ctgatataga tgtaatatat gatttgattt
     5521 gtgatatgga aaattcaaaa ttggattatg aaagatttca agaggtcttt aaaaaatatc
     5581 ttgaagatga taggtatttc tgccttgtgg cggaacatag cgggacagtc attggttgtt
     5641 taaatcttcg gatagagtat caacttcatc atacagcaaa aattgcagaa attttagaat
     5701 tagcggtaat ggataaatac cggtctaaag gggtaggaaa gaaacttttt gagaaagcat
     5761 ctgaaattgc gaaagacaat gagtgtcttc aaattgaggt ttgttgtaat caattaagat
     5821 caagagcaca taacttttat gaaaaacaag gcatgcataa ctttcattat aaattttctt
     5881 taaattttaa ggaggaagaa attaaagaga acaggctagg attataacaa ttatctcaat
     5941 gataatcgga cacatcattc ctgcccccat gcagagcttg tcagagattt gaacctaata
     6001 tagacaatat ttatttaagc ggcacatgac aagtccgggg taccggaaac ggtatagtaa
     6061 gatataggga aagtccctgt ggggccctct gaaatttact ggtttaatat tttgtattgg
     6121 aaatttatta ggaagtgaag tgataaagac tatgaaccga ctaatagata agtacacata
     6181 ccatctgcag cagggcgaaa tacaagctgc gtacaaaggg atattagagt ttatcggcaa
     6241 attgagagca gacattaaaa aaaatatccg cattacgaca taggcagttt atatcaaggc
     6301 tatacggaca tgagctattt ttcagtaagc acaaaaacac ttaaagataa aggattaaaa
     6361 ttctccattg tttacttaca cgcaaagaaa tcctttgaaa tatggctttc cgctcgtaat
     6421 agagatattg caaagagata tgagtctatt cttaaaagta agcaagagta aaggatgaat
     6481 gccatgtata catttacaaa ccagtttatg tcaaataata aaaatgttga gttccttaaa
     6541 gccaatatga tggggccaaa cgccatgcgg ttagcagagg aattggcgtc acacctgaac
     6601 attaatgaga atatgcgaat tcttgacctc ggttgcgggt gtggcctttc cacgcttttg
     6661 ttagtaaaaa aatacggtgt gtctgttttt gccgccgacc tttggatttc accaactgaa
     6721 aactatgagc gttttgagtc cgttggaatt gatgaaaagg ctgttccaat tttttcagac
     6781 gcaaccaaag gcttaccttt cgcaaacgga tatttcgact tgttgttcac cgttgacgct
     6841 taccattatt ttggtgatac ggcggaaatg cttccatcgc tcattccttt tgtgaaaaag
     6901 gaagggcata tcgccgtggc tattcccggc ttaaaatacg aattcgggga aaatgtccca
     6961 gatgaaatgc agccgttttg ggatagcgac atggaaagaa ccttgcactc tctcgattgg
     7021 tggaaagatt tatggaagag tgcaaagggc attgaagtga tggacattcg tgaaatgggc
     7081 tgttgcagac aggcttggga agaatggctg gccagtgatc atcccattgc tgctgaggac
     7141 atcaaaatga tggaggctga gggtggaaag tattttaata ttattcagtt gatagctaaa
     7201 gtcaaaaggt agtacagagg atatttatct tatggggggg ttaagagtgg aagtcaagcc
     7261 tattaaagga aataaaaagc tttttttaga tttactacta ttagcagatg aacaagaaga
     7321 tatgattgat aaatatttag aacggggaga agtatttgct ctatatgatg gagatttaaa
     7381 aagcatttgt gtagtaacgc aggaagatga aaatacctgt gagctgaaaa acttagctac
     7441 atatgaaaaa tatcgcggaa aaggttatgg aaaataccta ataagttata tatttgatta
     7501 ctgcagggat aagtgtaaaa cactattagt cggtacagga gacatcccgt ggataatttc
     7561 attttatgaa cattgtggat ttgaagtatc tcataaagtt gaaaatttct ttacaaataa
     7621 ctatgaccac cctatttatg aggatggaat tcaattggtc gatatggttt atttaaagaa
     7681 agatttctga tagcgcaagg gtgcagatag caaagtcacg aagcgggttc aggaagtagg
     7741 cgcatgaaga agggtgatgc aaaggtggag gttgaaaaat gtttgcatat cacaaagggg
     7801 tgaaggaaat ggaagtcata tctataaaaa aatctcctga gtataaagat agggcaatta
     7861 aatacttaca gagtaaatgg gcatccccaa atagtatgaa ggtctatgat gactgcataa
     7921 caaattgtat tactaccctg agtccattgc ctcaatggta tctgttgatt gataatgata
     7981 gaataattgg ctgcgcaggt ctcataacaa acgattttat aagccgtatg gatttatacc
     8041 cttgggtttg tgctgtattc attgaggaag aatacagagg aaatgcttat gggtcccttc
     8101 taatagagca ggcaaagata gacgcaaaag caggaggctt ttctcactta tacctctgta
     8161 cagaccatat tggatattat gagaagtatg gatttaagta tatcggtaca ggttatcatc
     8221 cgtggggaga cagttctcgc atatatgatg cagcgttata attagtgatt taagccaaag
     8281 ggagacaata tctgtacaag cggaacaggc aagaccccgg gtatcggaaa caatatggaa
     8341 ttattgctca gatcagggat gtcgtaagtg cagaaccgtt agatgacatg cagataaaat
     8401 tttaactata aaggaggaga gaatatgaga ttagttgaga tagagagatt cattataagc
     8461 ttgtttgaaa gcagtgagaa tgaacatttc catgaacaaa gcggaactac agtagagggt
     8521 acggaagaga ttagaagaat aggatattgt acaaatttaa ctcttgatac aattgaagaa
     8581 ggcaagaaaa ataacgttga cctgattata actcatcatg atgcatggga ttttatattt
     8641 ggccttaaag atgcatgccg ggaaaaatta tcggaataca acatatcaca ttattttact
     8701 catctgccat tagatgattg taattttggg acaaataatt ctttaattga aaagctgggg
     8761 ttaagtatta tcaagaaatc tcataatgaa gacggattct attgcggaag aatagctgag
     8821 tttgacgaag aggtagaatt tgaagagctt gtgagaaaat tggagatgat tcttggagaa
     8881 ccaacaaagg cgtggaaatt caaagatggc tgtataaaaa gagttggttt agtatgtggg
     8941 ggcgggggtt caactgcaga tgtaagagag gcatttgaaa aaaattgtga tgtatatata
     9001 actggcgaaa aagtccttta cactattgag tatgcaaaat ttgtaggaat gaatttaata
     9061 attggcagcc atacctttac tgaactattt gggattgaga gtctcgccag aaaaattgct
     9121 gacaagtaca gaggtgttaa tattataagg ttatccgaag agcaccttga agcataaaat
     9181 gcgtatccat aagataataa aagagacctt aataaatctg aagggtggaa attgctatga
     9241 aagcacaaat taaccttatt acaatttggg cagatgatat aaacaaaatg aaaaatttct
     9301 ataatcaggt gcttggattt aagattagta atgacctcgg aaattatgtt gaatttgaga
     9361 atgagggggt tcggtttgct atttgtttaa gggaagtcat gtatgcttac agtagtgaat
     9421 atgagaaaaa aactggtgga caggcatttg agcttgcctt tccttgtgaa gatcctgaag
     9481 atgtcgataa atcctttgaa tggctgattt cgaagggagc aactcctata catcaacctc
     9541 aaaatatgcc gtggaatcaa cgaactgcat tatttgcaga cctagaaggg aatattcatg
     9601 aaatcttcgc agaactctaa gaaacatagc attttcatgg aatgaatgta aaaggcttat
     9661 accgaatatc ccgcaatcct atgtacattg catatttcat ttgtttgtta ggatgtgtac
     9721 ttatatcaaa atcatgggcg ttatttacat tattgatagt ttttcaaata tcttcccatt
     9781 ggattatact atcggaagaa agatggtgca tcaagaaatt tggagaagaa tatatgagat
     9841 atatgaataa ggttagacgt tatatataga gccgcggcat cgttgccgca ctttacaccc
     9901 cttcaccgga aaggctgatc cgccagtata tcaataaaaa gtatgagggg aattgaatgg
     9961 ataatatgag atcagaactt attgccccgt gcggtatgaa ttgcaggctt tgcatggctt
    10021 ttcaacgtaa aaaaaacaag tgcatcggat gcaatagtga tgattgtaca aaaccagcgt
    10081 attgcctgca ttgtattatc aagaattgtg ttaccataca gaatagcagt tctggcttat
    10141 gctatgaatg tgataaattt ccatgcagaa gattgaagca actggataaa cgataccgaa
    10201 caaaatatca catgagcatg attgaaaatc ttgagaatat taagcaaaac ggtatgaaag
    10261 aattcctgaa gaatgaggaa gctcgatggg cttgcattga atgcggcaat attgttagcg
    10321 tacacagaaa tgcttgtcct atatgtaaaa cgcaatatat agagtaacag cagctatact
    10381 gcatactttt agaattgtaa tatataaagt acatgtgaaa gagaggctat atgatgagga
    10441 gtgttaagaa aatattttgg gaaaacccat agaagggttt gccaaagttc cctgcggagg
    10501 gacgcattta aaaaaaacag gagaaattag ttctatatcc ttaaaaagaa acaatttggg
    10561 cagtggtaaa gaaagaattg agatatacat acaatcctaa ataatttacg aaggagagag
    10621 tgcaatgagt gcttcagtaa agcctaatat tacaatagat atacttgata aaattgatat
    10681 tcgggtagga accataatat cagttgagga cataaaagat tcaaataagc tggttaaact
    10741 ttcagttgat tttggtgact ttttgaggac tatattagtc gggatgaaag gtgaaagaga
    10801 gaatccaaaa gagatagaag gacagcaggc gttatttgtt gttaacctgg ctcctaagaa
    10861 aatggcggga gaactttccg aagggatgtt atttgatatt ggatatgcag atggtattgt
    10921 tcctgtattg gctcagccgg aaaaagctgt acctaatggc acaagggctg gatagcgctt
    10981 attagttcgt aattatatgc aataaacacc gaaagaattt ttagggagat atctttatgt
    11041 tcaataattt aaaagcaatc aatgaaaggc caaaaccatt tcagtattat actgccgcag
    11101 aactatggac cgatgaacat acatcaaaaa agatgctgga ttatcatctt aatgaagaga
    11161 tagacgtttc ctctcgaaat gctcgtttta ttaaccgatc tgtatcctgg ataatcgaaa
    11221 agtttaatct caataacaaa tcaaatgttt tagattttgg atgcggcccg ggactttaca
    11281 ctaataggtt tgcagaaagg ggtattaacg ttaccggagt agacttttca aaaagatcaa
    11341 ttgagtatgc cgcacagata gcttcaagca aaaacttgaa agttaattat gttcacaaaa
    11401 attatcttga gtatgaaaca caagacaggt ttgatctaat tacaatgatt atgtgtgatt
    11461 attgtgcgtt gagtcccgaa caaaggggta ttatgttaaa caaatttaaa gagcttctga
    11521 aacattcagg aaatgtttta cttgatgtct actcattaaa atcatataac cggagagaag
    11581 aaatcgccgc ttatgaattc aaccatctaa ataatttttg gtctccggag gattattatt
    11641 gctttgttaa tacgtttaag tactgcgatg agaaaataat acttgataaa tacacaatta
    11701 ttgaaaaggc tcaaacgcga gttatatata attggctgca gtattacagt gttgaaacac
    11761 tcagaaggga atttgaggac aatggccttg aggttttgga gttttattcg gatgtttgcg
    11821 gactgcctta cgatgcagag tcattagaat ttgctgttgc agcaaggcaa aagaactaat
    11881 ggagtatagt atagcgtact tactgcatca aacaatgccc tcacgctgcg gcatgggcgg
    11941 caatatggtt atagctgtac ctatcgaata tcatgaagta atgaaaagta cccgggcggt
    12001 attgatcaac aaaagatgat gcttgaaatt gaaaaccata aagtagtaaa gaaaggcaag
    12061 cgatattttg cggaagacta tgaaaaatga ttctttcaac tgcagtgatt tatttgaagg
    12121 agaagaatat gacaaaaggt gaaaaagaaa aaatgtcatc gggagattat tatagtgctg
    12181 ttgacgaaga gctagtaaga gaaagagatt atgcaaggaa tttgacttac gagtttaacc
    12241 atacaagacc aactgaaaaa gagagaaggc tggaaattat tgtattgtcg ctattgccgg
    12301 gcattttgat tttagcattc tacttactgg ttagctggta cttggttccg ttttcaatac
    12361 cttcaatttt agttattttt gttgcaattg cagtaatttt ggttcccttt gaattgggat
    12421 ttttgattta tcaaggtaga aaggtgaaca aaagaatatc ccttaataat gtggtgctat
    12481 ttagagaaaa ggtgcctgtc attcaattta ttattcttgt cgtcatactg ataaagtgga
    12541 gtgaatttgt tttcagatgc ctgtctacca ggattgacac attattcgtt aacaattttt
    12601 tttattgggt cccgaaatgg tttttccttg atagctttta tcaacatatt agccaatact
    12661 ctaagacagt cttattggtt acgtggattc ttggcctgat ttttaacggg ataatcgggc
    12721 caatagtaga ggaactctac ttcagaggtt atttactgcc cagaatgaag catatgaaag
    12781 gatgggcgcc aataatcaac gtattgttgt tttctcttta ccacttcttc actccctggc
    12841 agaatgttac tagaataata gcactattac ctcttgttta tgtggtttgg tggaaaagaa
    12901 atatttacat tggcattgta acacactgtg cattaaatat aactggaatg attgggatgt
    12961 taccgatagt tttaaagtaa aatcacttac gcctaacaat gcctcatgca accacacctc
    13021 ataaaccctc aataggtaat ttatagtaat taaaaggagg aatataaatg gttgagatca
    13081 taaaagcgtc ggaagaatac gctgaaagct tctatcaagc gcttgacgtc gtagcaaggg
    13141 aacgtaagta tttaggcatt gtcaagggat ttccgctgca aggggtaaaa gattttgtca
    13201 gtatgaatgt agcaaatgac tatgcccaat attatgctat tgatggaaat aaagttatcg
    13261 gatggtgtga cataattcca aaaagaaatg aaggttttac tcatgttgga atactaggca
    13321 caggcttatt gagcgaatat agaaaccagg gtataggcag caagctgatt gaaaaggcta
    13381 tggaacatgc agtgaagaag aatggtattg agaaggttga acttgaagta tttgaaagca
    13441 atatcaatgc tataaggctt tatgagaagt tcggatttgt ttacgagggg aagagaataa
    13501 aatcacgcaa gcttgacggt gtttatgata acgtggtgat gatgggaaag gaagtattat
    13561 gattgaaaac aaacactatg ttttcggatg tagaaatcaa attaaagaaa agaaataaaa
    13621 tattgttttc ccgcgacagt aaatgtttgc aagagctgat aaaacttatt caactgcaga
    13681 atcaccgaac cctggtgatg tgggcactgg attgtgcaaa actgcctttg gagcaatttg
    13741 aagcaaagta tcctgatgaa cggaggccaa gaatatgttt ggagctttgt gaggcttggg
    13801 caagaggcaa aatcaaaatg cccacggcaa aaaaggctat cttgaattca catgcagcgg
    13861 caaaagaaat caatgacagt gaatatggcg ctttgtgcca cgcaatcggt catgccggag
    13921 caaccgtgca tgtagggaca catgccttgg ggctgcctat gtatgaattg acgtcaattg
    13981 ttttaaggta cggaaaagat aactttttaa taccggtcag tgaaaagata aattactact
    14041 ataaacgcct gttgtactgg caagaaaata ccgataaact taaacttgat tgggcggact
    14101 ttttgttaag gggtgatttt aatgaatgat tatcagattg gcaggccaaa gccggaggaa
    14161 atatgctcta taaacgagtt tttcgaaatc gtggtcaggg atacctttgc gaaaaatggt
    14221 atttcccatt tgacggagct gatggaaggc gaaattgagt gcaagaagaa gtatcttatg
    14281 caggatatgg atagtgaagg ccgtgatagg ttttttctgg tcgtaaggga caaaggcagg
    14341 atcataggca ctattgaata cggcaggcca aatgaattga ttatgaaatg cacaaaggga
    14401 agatacattg acgttataga aatagggaca gtgtttgttc atccggatta tcaatataaa
    14461 ggaataggaa gcatgatgct caggatgatg tacgatgagt tgaaaaataa gggatatacc
    14521 gagttttgcc ttgacagcgg ttataagact gcacagaagg tttggaccgg caagtggggc
    14581 aaaccgtctt gcataatgga ggactactgg ggcaagggag cacaccatct ggtatggcgt
    14641 gtagatttgt agccctttca tatatcactg cagccttgct atcggcagtt tttcaacctc
    14701 atcctctttt gggaacctgc ctgcactctt ttttgagaaa atcagctcat tattcagttt
    14761 aacttttaaa acccccccgg aggatggaat caatgtaaga gaggatatac tatttttatt
    14821 atatgtattt aaataaaaaa gtaagattgc aaagcaactg cagttatagt atgtattatg
    14881 tatggcctta taggcggatt cctcggggga atacttgggc ttattgcttt aggcataccc
    14941 gggcttataa tcggacagct tgcaggggtt atactgggag cactgtaaac aggaggagcg
    15001 gcatatgagc aactctgtaa ccgggttctg caaagtcaga aataatttga caaaaactgc
    15061 agcattaaat atactattaa aaatacgttt tttaaactca tataatttcg aggatacggc
    15121 tcggaagtat ctacaacata accgttaatt atgtttctat gagtgaaatt tcttgtctgc
    15181 aatttcacta ttgcagactt ttttctacaa attaaaaagg agtgtgttta agcccaatgg
    15241 aaaagtttgt gaaagagggt ctgacctttg atgatgttct actggttccg gcaaaatctg
    15301 aagtacttcc aaagaataca gacacctcga caatgcttac caggaaaatc aagctcaata
    15361 ttccgctgat gtcggcaggg atggataccg ttacggagtc aaagcttgct attgcaatag
    15421 caagggaagg cggtatcgga ataatccata agaatatgtc catagaaaag caggctatgg
    15481 aagtggataa ggtaaagagg tcggagcacg gagtcatagt tgatcccttt cacctgtccc
    15541 ccgatcacgt tatatccgat gccatggagc tgatggaacg gtacaggatt tccggagtcc
    15601 ctataactga tgaaaaaggc aaactggtag ggatacttac aaacagggat cttcgctttg
    15661 agacggagtt ttcaaaaaaa atatccgagg taatgacaaa agaaaacctt ataaccgcac
    15721 ctgtaggcac caatttagag caggcggaac agatattgaa gaaacacaag atcgaaaagc
    15781 tccctcttgt ggatgataaa ggatatctta aagggctgat tacaataaaa gatattgaga
    15841 aagccataca atatccaaat tccgcaaagg atcccggagg aaggcttctt gtgggcgcag
    15901 ctgtcggcgt aaccgaagat atgattgaga gagcagaggc tctggtatca tcaaaggtcg
    15961 atgttgtagt aatagatact gcccatgggc attcaaaggg tgttatagaa gctgtaagga
    16021 ggataaaagg ccgattccct gaactgcagc tgattgccgg taatgtagct acagcagggg
    16081 cggtaaaaga gctaatcgag gccggtgcgg atgctgtgaa ggtaggtata ggccccggtt
    16141 cgatctgtac caccagagtg gtgaccggtg taggagtgcc tcagataacg gctatatatg
    16201 actgtgcagc ggaagcagat aaatatggca tatccataat agcggatgga ggagtcaaat
    16261 acagcggaga tatcgtaaag gcaattgccg caggagcaag tgcagttatg ctgggcagcc
    16321 tttttgccgg tactgcggaa agccccggag aaatggaaat ttaccaggga agaagcttca
    16381 aggtatacag gggtatgggt tccttgggcg ctatggcatg cggcagtaag gacaggtatt
    16441 tccaggagga tgcgaggaag cttgttccgg aaggagtgga aggcagagta ccttataaag
    16501 ggcctttggc agaaacgatt tatcaattgg taggaggcct gaaatccggt atgggctatt
    16561 gtggaacaca tactattgag cagctgagga aaaatacgca gttcatgcgt ataaccgctg
    16621 caggtttgag agaaagccac ccccacgatg taagcataac aaaggaagct cccaactata
    16681 gtctgtaggg aactaatgtt atctatatta ggaatatgca aagatgcacg ggctgcgagc
    16741 cgtgtgccgc gaggcagcta atgaatggag agtgtaatat ggcagacaag gaactggtat
    16801 tgatactgga cttcgggggt caatacaatc agcttattgc ccgaagagta agagaggcca
    16861 atgtatattg tgaagttgtg ccttatgatt acagcctgga tgaaataagg aagaagaatc
    16921 ccaaggggat aatattcacc ggcgggcctg caagtgtata tgaagaggag gctcccaagt
    16981 gcgatccggg aatgtttgaa atggggatac ctattctggg catctgctat ggagcgcagc
    17041 ttatggcaca tatgctggga ggaaaggttg caagggctgt taagagggaa tacggaaaga
    17101 cgcaggtgaa gcttctggag aatactcttt tcagagaagt accacaagac agtatatgct
    17161 ggatgagcca taccgtttat gtagacagcc ctccggccgg atttgacatt attggagcca
    17221 cggagacatg ccctgtagcg gccatgggaa gcaagtccgg gaagttgtac ggagttcagt
    17281 tccacccgga agtagagcat actgagtatg ggaaacagat attgaggaat ttcctttatg
    17341 atatatgcgg gctttcaggg gattggacaa tgggaaattt cattgatgaa aatgtagagc
    17401 ggataaaaga acttgccgga gacaagaagg taatatgcgc cttaagcggc ggtgttgatt
    17461 cctcagtagc ggcagtattg gtgcataagg ccataggaaa tcgcctgacc tgcatatttg
    17521 tcgaccacgg cttgctgaga aaaaatgaag cggagcaagt agaagaagta ttcaggaaac
    17581 gttttgacat caaccttatt aagattaacg cccaggacag attccttgac aaactgaagg
    17641 gagtatcgga acccgagaga aaaagaaaga taataggcga agaattcatc agggtttttg
    17701 aggaagaggc aaataaactt ggagagattg acttcctggt gcaaggtaca atttaccctg
    17761 atgttattga aagcggaacc aagactgcgg ctgtaataaa gtcccatcat aatgtgggcg
    17821 gcctgcctga aaacatgcgg ttaaagctta ttgagccttt aagggaactc ttcaaggatg
    17881 aggtaaggct ggttggcctg gagcttggcc ttccggagga tttggtatgg aggcagccct
    17941 tcccgggacc gggacttggc ataagagtat tgggagaaat aacaaaggaa aagcttcaca
    18001 tagtaaagga aagcgatgca attctcaggg aagagataaa gaatgcagga ttggaaagat
    18061 gcatatggca gtactttact gccctgcccg acataagaag cgtaggggta atgggggacg
    18121 agcgaaccta ttcccatacg gtggcaatcc gtgccgttac cagctccgat gccatgactg
    18181 ccgattgggc aaggataccc tatgaagtac ttgagaaaat atccaacagg atagtaaacg
    18241 aagtggagca tgttaaccgc gtaatgtatg atataacaag caaaccaccg gcaaccatag
    18301 agtttgaata gaagtacatg aagtccagaa aagtgaagta caccccaaat gtcagataaa
    18361 aaatctatca tttggaggtg tacttttttg cgggacaggt cggatatata ctactggcag
    18421 aggacggaaa taataattga catatgtcat aaatacctta taattgaaat tgaaggaatt
    18481 aacttaaaaa taattaaggg gggttttcta tgtcatatag tccatcctta ggaagttctt
    18541 taaccaatac aaaaatgagg actccggagc atatttccga tttctcggga atgtgttcgg
    18601 tatgtactgc aagctgcatc ggaacttgtg aaataggcct gtctgctgtc agaggttcag
    18661 aggccatata tccctttgaa acagacaaaa accagtttgc ctctgaaaaa agctatcctc
    18721 tggatttttc ccactttaat atcaacggca gggtctttgg cgcgctcggc tgcccggagg
    18781 acgcaaatga agcaacattt cccaaggcca atattgctgt ggatttcgga atcaacaaca
    18841 aaattaaatt aaaggcgcct ataattcttc ctgccatggc caagctgaac tggaaggatt
    18901 attatgcagg agctgcattg tcaggggtgc tggttgttat tggagaagat gctgtggcga
    18961 aagataagga actggtactg gaaaatggga aggtcgccgg ctctccgctg ctgaaggaaa
    19021 tggtatcatc cttcaggcag tattacagag gatttggaga cataatactc caggggaatt
    19081 acgatgatga aaacctcggc gtattggatt atgcaattaa agaacttggt gtaaaaaccg
    19141 tcgaactgaa gttcggacag gcagccaagg gaattcaagg aatggggaga gttaaggata
    19201 tagatgctgc tcttgaatac cagaagaagg gctacttagt ttaccctgac ccgtcggatc
    19261 ctgaaatagc aaaaaattat aaagagggaa aaggacaggt atttgagaaa ataggcaagc
    19321 ttcctatgtg gaacgaagat atactgatta atcgggtaaa agaattaaga aagcttggag
    19381 ctgagcgcat atgcttcaag acaggcccct ttgaccctaa ggatttaata aggattctta
    19441 agatagcttc aaaggcagga gtggatttgg taacctttga cggagccgga ggaggatcag
    19501 ggaacagccc cgtcaagatg atgaatgaat ggggaattcc gacagtatac ttggagagcc
    19561 tgttgtataa cattttaaag agaatgaagg agaagaacta taatcttccg caggttgcga
    19621 taaccggtgg ctttgccatg gaggatcagg tatataaagg attggcgtta ggtgccccat
    19681 atataaatat tgtcggcata ggaagggcgg caatggcagc agccatggca ggcaagcaga
    19741 taggagattt aatgaaggaa ggaatgactc ccaaagaata tctgaggttt ggcactgcta
    19801 aggaagaggt attcggagac attagagagc ttaaactgct gtatggggaa gatgctttga
    19861 atatttcaac cggagcggta gggttatatt catacataaa caggatagct gcagggctaa
    19921 agcagcttat ggcactgaac aggaaattct ctctggaata tattgacaga agcgatataa
    19981 tccccttgac cgaaatagct tcaaaggtta caggattaga ggcagtatac cggtataata
    20041 acgagttgga taaggaaatc taattcaaat tagttattga catatgtcag aaacatagta
    20101 taataaaaat tgaaagcgac atatgtcata aactatttgg aggtgaatca tatgccagga
    20161 agagatggaa caggtccgat gggagccgga gcagctacgg gaagaggttt aggcttttgc
    20221 agcggagtta atgcagccgg attgggattt ggatgcaggc gtggttttgt aagaggttac
    20281 ggaaaaggct ttgctgtaaa ctgggcttcc gcaaaaacac aaaaggagat gctgcaaaca
    20341 caaaaggact tgctggaaag ccggctcaaa atcgttaatg aacagttgga gaatatatag
    20401 ccagaagcag aaataaccgg aggacgctcc agttatttct gctgttaaga gaggtgaatc
    20461 acatgccaag accaagaaaa tggagaaaag tatgctgcct gcctgaaagc aaccgcttcg
    20521 gccctctggg gtccccggca gacgaatcga attacattgt tatgaccgtt gacgaatatg
    20581 aaaccataag gctgattgat ctggagggat ttacacagga agaatgtgcc agccggatga
    20641 atattgcacg cacaaccgtt caagggattt atgtcgacgc aagaaaaaaa cttgcagagt
    20701 cactggtaaa tggaaaggtg ttgtggattg aaggcggaga atacaggctt tgtgacggca
    20761 gcggcaatgc ctgcgggaga ggcggatgcc gcaggcaaag attcggcagt aaatagaatg
    20821 gaggattttt atgaaaattg caatacctgt agacgataaa aatatggaag caagtgtatg
    20881 catatccttt ggacgcacac catattttct gattcacgac actgaatcaa atcgaagtct
    20941 gttctttgaa aacagcgcag cagccagcca gggaggtgcc ggtatcaaag cggcacaatt
    21001 ccttgtagat cataaagctg atatattgct gacaccccga tgcggtgaaa acgcagccga
    21061 ggttataaaa gctgcaaaca ttaaaatgta taagactata cccggttctg ccaaagataa
    21121 tgttaatgca tttactgaag gaaaattgtc tttgcttgaa gaaatacatg ccggatttca
    21181 cggccatgga gaaaaataac atgaagattg ctgtgttaag cggcaaggga ggcacaggga
    21241 aaacactgat atcggtgaat ttggctgcag cggctgggaa atctgtttat ttggattgcg
    21301 atgtggaaga gcccaatggt catctttttt tcaagccgga gaatttttat gaggaagagg
    21361 tttcaattaa aatccctgtt gcagatgaaa agcaatgcag cggatgccgg gtttgcgttg
    21421 acttttgcaa attcaatgct cttgcttata taaaagacag actggtagtt tttgatgagg
    21481 tttgtcattc ctgcggcggg tgtattctgc tttgcccgga aaaggcgctg aatgagaaag
    21541 agaaaattat agggaaaatt cagaagggtg tttcaggtca agtgactgta aatacaggaa
    21601 tactcaatac cggtgaagct tcgggcatcc ccctgattaa gaagctgctg gatgaaaaca
    21661 gaaaagtgaa aggaatgact acctttattg attgccctcc gggcagtgcc tgtatagtaa
    21721 tggagagtat cagggatgcg gactattgta ttctggcagc tgaacctaca ttgttcggcg
    21781 ttcataatct taatatggtt tatgaactgg taaagctgtt tcgcaagcct tatggggtgg
    21841 ttcttaacaa atgccaggag ggagaaaacc ctgcagagag gttttgtgaa gaaaaaggca
    21901 tcaggatttt aggcagaatt cccttcgaca atgagcttgg aacactgaat tcaaatgcaa
    21961 agatagcagt aagagaaagt gaaaagtaca gaaccgtgtt ttcatctctg cttgaaactg
    22021 tgactaagga ggtgcggcat gaagcagctg ttaatcctta gcggaaaagg cggaacaggt
    22081 aaaacaacaa tagccggtgc atttataaag ctttcccatg caaaggccta tgcggattgc
    22141 gatgttgacg cacctaatct ccacttgatt atgggtcgga attcagaacc ggagaaaaga
    22201 gatttttacg gtctcccaaa ggccgaaata aatcaggaat tgtgcatacg gtgtgatcgg
    22261 tgcaggcaaa attgccggtt tgaagcaata ttatcggata aagcatataa ggtggattat
    22321 tttgcctgtg aaggctgcgg tgtctgtgaa gccgtatgcc ctgcgggagc agtttcttta
    22381 aatgatgctg ttgcagggga attgatgctg tattcagatg aagataaagt tttttcaaca
    22441 gcccagctta aaatgggaag cggtacttca ggattgctgg ttaccgaggt caaaaagcaa
    22501 atgagattaa atgcaaaaga tccggagctt gccattattg acggctctcc cggaatagga
    22561 tgtcctgtta ttgcatcact aagcggtgct gacatggtat taatagtagc agagccttcc
    22621 ctctcaggca tcagtgacat ggagcgcata gaaaaaactg cggcgacatt tcagacaaaa
    22681 gcagcagttt gcgttaataa atatgatgca aacattgcaa atacggcgag gatagaagaa
    22741 tattgccgga caaataatct gccctttgtg gggaggatac cttttgatcc ggaggcagtg
    22801 aaagcgatta atgagggcct tactgttatc gatattgatt gtccggcagg caatgcagtc
    22861 agagaggttt ttaacaaaac cacgaagctg ctttttgata cttcaacaat ataatattta
    22921 catgaaggag acaaaaaaat gagcgaacaa tgcaatcaga actgcagcag ctgttcagaa
    22981 agctgcactg aaagaaaaga agaatttgat ttttcagaga aaccacatgc attaagcagc
    23041 attaaaaaag taatcggtgt tgtcagcgga aaaggaggag taggcaaatc attggttaca
    23101 tcaatgctcg ctgtcacaat gaacagaatg ggctatcatt cagccgtatt ggatgccgat
    23161 attacaggcc cttcaatacc caaagccttt ggtataaggg aaaaggccag aggcagcgaa
    23221 aagggattat atccgataaa aagtcataca ggtattgaca ttatgtccat aaatctgctt
    23281 atggataatg acaaggaccc ggtagtatgg agaggtccca tattggccaa tacggtaaaa
    23341 caattctggt cggaggtcat atggagtgat gttgatttca tgttcattga tatgcctccg
    23401 ggaaccggtg atgtgcctct tactgttttt caatcaatag caatggatgg aatcattata
    23461 gttacatctc cccaggagct tgtttcaatg attgtatcca aggcggtcag gatggcggag
    23521 attatgaata ttcccataat cggcctggtg gaaaacatgt cgtattttaa atgtcctgat
    23581 aatgataaag aatacaagat atttggagac agtcatatag atgaaattgc cgaagaacat
    23641 aatcttaagg ttcttgccaa gctgcccata gacccgagac tatctgcagc ctgtgacaaa
    23701 gggatgatag aactttttga aggcgattgg atggagcctg tcgggaaaat attagaaaat
    23761 atggaggata aaaaatgatt aagatagcgg tagcaagtga aaatgatatg gtaacggaac
    23821 atttcggaca ttgcatgaac tttaacatct ttgaagctga gaatgaccgg atagtaaaaa
    23881 gcgaatctat tttgaatccg ggacataggc ccggttttct ccccaacttt ctgaataata
    23941 tgggagtaaa tgtaattata tcaggaggta tgggcggcgg cgccattgac atatttaacg
    24001 aaaaaggtat agaggttgtt ttaggtgcaa ggggttatgc caaggaagct gcggaagcat
    24061 acctgaaagg cacattgaaa tctaccggtt ctgtttgtca tgaacatcag catcatgatg
    24121 aatgcggaaa ataacaagga ggatttgcct atgacagcgt ctctatatgt tattgcgata
    24181 ttggcacttt cggtctcact tctcaaaagc aaggaaagaa ctatacttgc tcttaagaag
    24241 gcatggaagt cttttgaaaa catactgcct caatttttgt ctattttgat tataataggt
    24301 atattattgg cagttttatc acccgaacag atctcaaaat tattgggaag agaatccggc
    24361 tggtacggtg tgctgattgc tgcagttata ggttctgtta ctttagttcc cggatttatc
    24421 gcatttccat tggcggcagc attgttaaaa agcggcgccg gctatatgca aatagcagct
    24481 tttatttctg cgctaatgat ggttggtatt gtgacgattc cggtggaggt ggaatacttc
    24541 gggaaaaggg cagccgcggt taggaatact gccgcctttg tattttcttt gattgtagct
    24601 ttggttatgg gggtggttat gtgacgacta aagaagcggg gttggttaaa agatatagag
    24661 tatttttttt acttgcttta cttaacatgg ctgtactcat tttttatcct gcggttggca
    24721 gcaagtcatt gcagctgaca tggtcgaatt tgcttgagat gatggcaata ttgcctccga
    24781 tttttattct gcttggactg ctggatgtat gggtgaagcg tgaaaccatg atcaagtata
    24841 tgggtgacga ttcaggaata attggtgtac tgctggcatt ctttcttggc tctgccgctg
    24901 ccggcccgct gtatgcagca tttcctgttg ccggtgtgct gctgaaaaaa ggaagcaaat
    24961 tgtcaaatgt gtttattatg ctgggagcct ggtcaaccac aaagataccc ctgatactat
    25021 ttgaagcctc ttcccttggc ctgaagttca tgcttgtaag aatggtcatg gatttgatag
    25081 gaatagctgt tattgcatat tttaccgaaa ggatgttgtc taaaggtgaa attgaagcaa
    25141 tatatcaaaa tgcatcaaac cgttagcctg aggggtattg agtattgacg gcatacatgc
    25201 tgatataata gaatacgtat aataatatta aatatttggc agtagtgata tggaggtggt
    25261 tcaaatggaa gatcagaaat cagcccgtga gaaacgcaag gatgagcatc tgaagtattt
    25321 tcttttgagt aagccttcag gatacaacgg cttttccgat gtggtactgc agaataattc
    25381 cctccctgat gccgattttg atgaaataag cacagagtgt gtgttcctga acaaatatat
    25441 tgacagcccc ctgatgatta atgcaatgac cggagggaca gagtattccg gcgatattaa
    25501 caggaagctg gcaaggcttg cggtcaaatt caacattcct atagccgtgg gttcacagac
    25561 ggtagctttt gacaatccca aggctgctga tacctttaag cgggtaaggg atatacacag
    25621 ggatggaata gttattgcca atctgggtgc aaaccgggat atagaggatg tttacaaggc
    25681 aatagacatg attgatgcag atgcggtaca gcttcacata aacatagtcc aggaaatgtg
    25741 catggcggaa ggagatcgga acttcagggg tgtgctggag aatatcaaaa agattgccgc
    25801 actggctgag gttccggtaa tagttaaaga aacaggcttc ggaatatcct ttgaaactgc
    25861 cggaaagctt caggcggcag gagtaaaata tatagacatc ggaggcaaag gcggaactga
    25921 ctttgtaagc attgaaagct tgcggaacag cgatagggaa tgccccttcc tggagaattg
    25981 gggcataccc actgctctga gcctttttca gtgcagaaag gccagcagtg atatgttcat
    26041 aatctgctcg gggggcatat caaaggcgga agaagtggtt aaagccctct gcatggacgg
    26101 agacatggcg gcattgggag gaacaatact gaggatactg ctggagcagg gctatgatgc
    26161 tgcagaaaag tacatggcag cccttttgta cgaaatcagg gtgcttatgc tgttattggg
    26221 agccaaggat ataacagatc tgaaaaacgt atcttacctg ctgaaagggg agcttgggga
    26281 gctagtgctc tccctcaaga agctctataa attttgatgc tgctacaggc agcggaatat
    26341 ttgaatgtat gacgaatccc agatttcttt cggggatttc ttcttttata tcaattatga
    26401 atacatcatc cttcgcaagc ccaatcctga cacattcatc cggcacgaag gctattccga
    26461 gtcctatctt tgacatttcc atcagtatat ctatactccc aagctcaatt tccggcttta
    26521 tggatacctt gtgctttgcc atcaggctgt cgaagaattc tctggtggtg gagttcctct
    26581 ccaatacaag aagggggtac tcttccagtt ccttcagctg caccttcctg cctttcagct
    26641 ctttaaaatt ttcatttgct atgaatacat ccctgactct tttggtattt tttacaataa
    26701 tattgctgta attaagtttt tcgggaatgt ttataacgct cagatccaca ctgccttttc
    26761 tcaaaagctc tatgcacctg ggggaagtcc tgtttgtcag gtgaatcttt atattggggt
    26821 aagcctggtt gaagcttttg ataaagggca taagataata tttgcatatg gtatcgctgg
    26881 ttccaatcct gatttcaccc tgggtaaggg aatttctttc ctcaagtatt ctttcaccgg
    26941 attttatgaa attaaaggct tgctcaatat gactatataa tatttcccct tccctggtaa
    27001 gttttactct cttggtattc ctgaaaaaaa gcttacactt gagtttttcc tccagaagtt
    27061 ttatggactg gcttactgcc gactgggtta tgaaaagctc ctccgaggct tcggaaaaac
    27121 tgagggattt tgacacataa taaaaaacct tataaagttc aaagttaata tccatatata
    27181 agcccttctt atcattaata ttaaaaatat taattatttt aaataatttt actatgataa
    27241 aatcaaattg taaagtaggg acaccataca tggtgtccgc atcatacatg gtgtccgcag
    27301 aaaccggtga ataacagcac cgtattaaag agatattagt ataaatgagg tgaagtggga
    27361 tgaatggcaa tgtaagaagg atatttgtgg agaaaaagga cgattatgcc gtttccgcaa
    27421 aggacttgtt caatgatatc agggaaaacc ttgggataaa gagcctcaca aatcttagga
    27481 tagtaaatcg ttacgacatt tccggaatca ccgacagtga atacaattca gccaagaata
    27541 caatcttttc agaacctccc gtagataatt cctatgatga aaaaatcata atcgataaaa
    27601 atgatagatt gtttgcaatt gagtaccttc cgggacaatt cgaccagagg gcggattcag
    27661 ccgcccaatg ccttcagata ttgacccaga aggaaaggcc caaggttgtg actgcgaaac
    27721 tgattatact ttcgggagaa atttccgatg atgaatttga aaaaatcaaa aattattgca
    27781 ttaatcctct tgaattaagg gaagcaagcc tggaaaagcc tgaaagcctg gaggctgttt
    27841 atccggaacc gggggacata aaagaagtca aagattttat aaccatggat gaggccggac
    27901 ttgcaaggct tatggaagtt atgggttttg caatgagttc cgaagacctc cggttctgcc
    27961 gggaatactt caagacgtcg gaaaaaagga accctacggt aactgagtta agagtaatcg
    28021 acacctattg gtcggatcac tgcaggcata ccaccttcaa cacattaata gaaaaaatag
    28081 aaattgagaa gggcaaatat accgaagcta ttgaaaaagc cctgttaagg tatatggacg
    28141 caagagcctt tgtatacgga gactccgaaa gggatatcac ccttatggac atggcagtaa
    28201 tgggcatgaa agaaatgagg aaaaaaggac ttcttgatga ccttgaggtt tccgatgaga
    28261 taaatgcctg cagtattatc gttaatgcgg atattgacgg gaagcgggag gagtggcttg
    28321 tgatgttcaa gaatgaaaca cacaaccatc cgacagagat agaacccttt ggaggtgcag
    28381 caacctgtct cgggggagca ataagggatc ccctgtccgg caggtcctat gtgtacggtg
    28441 ccatgagggt tacgggaagc gcagacccaa gggcaagcat agaagaaacg ctgcccggaa
    28501 aacttcccca gaggaaaatt accacagggg cagccgcagg ctacagctca tacggcaatc
    28561 agatcggcct tgccacagga caggttgcgg aggtttacga cgaggacttt gtggcaaaaa
    28621 gaatggaaat cggcgcggtt ataggtgcgg cgcctaaaag caatgttatc aggaaagcac
    28681 cctcagaggg tgatgtaata atactcctgg gaggaaggac cggaagagac ggctgcggcg
    28741 gagctacagg ctcatcaaag gagcataccg aggagtcgat acagagctgc ggagcggaag
    28801 tgcaaaaagg caatgctccc acagagagaa agattcaaag actgttcaga gaccccatgg
    28861 tcagcaggat gataaaaaag tgcaatgatt tcggtgccgg cggtgtatcg gtggcaatcg
    28921 gagaactggc cgacgggctt ttaatagacc ttgacgcagt tcccaagaaa tatgaaggct
    28981 tggacggaac cgagctggcc atatcggaat ctcaggagag aatggcggtt cttttggctc
    29041 cggaggatgc ggaaagcttt ataaattatg ctgccgctga aaaccttgaa gcagtggttg
    29101 tagcgaaggt aacaggggac aacaggcttc ggatgcagtg gcggggacag acaatagttg
    29161 atataagccg tgactttttg aacaccaacg gtgtaagaca gagtacacgg gtgctgataa
    29221 agtctccgga tgcggagagc tgcttcaata agctgcaggc ttgggaaaag agcaaggagg
    29281 acttgaaggg aaaatggctt gagaacctta aggatctcaa tgtatgcagt cagaaggggc
    29341 ttgtcgaaag gttcgacagt accataggcg caggaacaat actgatgccc ttcggaggca
    29401 tataccagga aactccttcg gaaggactgg caatgaagct gccggtgctt tccggagaga
    29461 caaatacggc aacggtaatg acctacggat ataatccgaa gcttgcaaag tggagcccct
    29521 tccacggagc ggtatatgca gtggttgagg cggttgcaaa ggttgcagcc cttggagggg
    29581 attacggcag aataaggctt acacttcagg aatacttcga gaagctgggc aaggttcccg
    29641 aaaagtgggg aaaacctgcc ggtgcgcttc ttggtgcctt ctacgcccag atgatgctgg
    29701 gggttcctgc catagggggt aaggacagca tgtccggtac cttcaaggac cttaatgtgc
    29761 ctcctaccct ggttgccttt gcggttgaca cagcggaagc cggcaggata gtatcacagg
    29821 agtttaaaaa atccggaagc aaggtgatac ttttaagggc tgaaagagac gacaaggacc
    29881 tgccggattt tgaaaagctt cataagatat tttccaaggt acatgaattg atacagaagg
    29941 gtttggtgct ttcatcagca acagttagga ttggaggaat tgcggcagca gtcagcaaaa
    30001 tgtgcttcgg gaacaagctg ggctttgcct ttgaaaaggg ctatgaggaa agggatctgt
    30061 ttactcccga atacggttca attattctgg agatggatca gtcctgcgac ctgaacggcg
    30121 tattcagcgg catggattat gaaattatcg gagtcacgga aagcaccgga tatataagca
    30181 taaaaggtat aaaaattgag cttgaagaag cgcttaaggc atggaaagct cctttggaga
    30241 ggatattccc aacccattca ggggataatg atgacaaacc gcagaatata tactataata
    30301 gtacaaatat tgtcaaatcc tccgcgaaaa ttgcacggcc caaggtattc ataccggtat
    30361 tccccggtac aaactgcgag tacgatactg caagagcctt tgaaaaagca ggaggcgagc
    30421 cttcaatttt cgtattcaga aatatcggcc cttcagatat agaagcctca ataaatgaaa
    30481 tggtaaggct aattggaaat actcagataa tagcacttcc gggaggcttc agcagcggcg
    30541 acgagccgga cggttcaggc aaattcattg cgacagtatt caggaacccc cgggttaagg
    30601 aagcggttat gaaattcctg cgggagaagg acggcttgat gcttggaatc tgcaacggct
    30661 tccaggtgct tattaagctg gggcttctgc cctacggaga aatcatggac atggacgaaa
    30721 gctcaccgac cctgaccttc aacaagatag ggcggcacgt atcctgtatg gtaaggacaa
    30781 aggtggtatc ctcactgtct ccgtggctca gcaagtccaa agtcggggat attcacacca
    30841 tagcggtatc ccacggggaa ggaaggttcg cagcaagcag ggaaattatg gacagcctta
    30901 taaaaaacgg ccaggttgcc acacaatatg tggatttaaa cggcaatccg acatacgatt
    30961 ctgcctttaa tccaaacggc tccatagaag ctgtagaagg aataacaagt cccgacggaa
    31021 ggattttcgg caagatgggg cattcggaaa gaaccggggc caacgtcggc aaaaatgtgc
    31081 ccagagacaa ggaacagctg atattcaagg cgggagtgga gtatttcagc tgataaaata
    31141 aatagcaata aaaaggagtg atgcttgtgt cttcagataa accaaaggct gcggtcataa
    31201 tgggcagtga ctcggatctg tccgttgtaa gaaaggcaat tgaaatttta aaggcttttg
    31261 aagtagacta tgaggttaat gtaatatctg cgcacaggac accggacagg gctgcggaat
    31321 atgcaaaaaa agcggaagaa aacggttttg aggttataat tgccgcggca gggaaggcgg
    31381 cgcatcttcc cggggttttg gcggcattca caacgcttcc ggttataggg gtacctataa
    31441 aatcgtcggt tttggacggc ttggattctc ttttgtcaat agtacagatg ccttcaggaa
    31501 tacctgtggc cactgttgcc gttgacggcg cagaaaacgc agggcttctg gcagttcaga
    31561 tcctatcggt aaagtacggc aatttaaagg ataagcttaa ggcctacaag gaaaagatga
    31621 aacaggatgt aattaagaag gacgagaaga taaaagagga ggttttctaa tgaaaaggct
    31681 gggaatgata tatgaaggaa aagcaaaaaa agtatacaaa acagatgatg aagcactgta
    31741 tatagttgag tacaaagatg atgcgacggc attcaacggc cttaaaaaag gaaccataat
    31801 aaataagggt gttatgaaca accggatatc ggaaaagctc ttcaggcttc ttgaagaaaa
    31861 aggcatagac actcatcagg ttgaaaggat aagtgacaga gaggtagtcg tcaagaaggt
    31921 aaaaataatt cctctggaag tgctggccag gaattatgct gccggctccc tttcaaagag
    31981 attaggcctt gaggaaggtg ttaagcttaa ttccacagtg ctggagtttt cctacaagaa
    32041 tgacgaactg ggagatccca tggtaaatga ttatcatata aaggcaatag gtatagcaac
    32101 agagcaggaa gtagcaaaga ttaccgaaat ggctctgaaa atcaatgaga ttctgagtga
    32161 atttatgctt accaaaggca ttctgctggt ggatttcaag cttgaattcg gcaagactcc
    32221 cgacgggaaa ataatcctgg cagatgagat ttccccggat acctgcaggc tttgggatgc
    32281 ggtgaccctt gaaaagatgg ataaggatag gttcagaagg gatatgggca aggtagaaga
    32341 aggatatatg gaagtagtaa aaaggttgtg ttaggagata agccatggac aaacttaagg
    32401 aagaatgcgg aataataggt atctacgaca aaggcaatat ggaaatagaa agccttacct
    32461 attacggcct cattgccttg cagcacaggg ggcaggagag cgcagggatt gccgtcaaca
    32521 gggacggggt cataacctgt tataaggaaa tgggcctggt gtccgaggtt tttgatgaca
    32581 agatattgaa ccttttaaag ggggatatca gcatcggcca tgtaaggtat tcaacaaccg
    32641 gggacagttt tgcccacaat gcacagcccc ttttggtcaa gtacaagaag gggagcatag
    32701 cccttgccca taacgggaat ctggtgaatg caggttcggt gcgggaagag ctggaggacc
    32761 agggtatgat ttttaccacc actatagaca gcgaggttat tgcaggcctt atagcaagaa
    32821 gctgcagcaa aggcattgag gatgccatac tggataccat ggacattatc cggggctctt
    32881 attccctggt tataatgacc gatgacaagc ttataggggt gagggacagt cacgggctga
    32941 ggccgctggt tataggaagg ataggaaacg gttacgtgct ggcttccgaa acctgtgccc
    33001 tggacaccat aggtgcaagc ttcataagag atgtaaggcc aggggagata ataatcattg
    33061 atgaaaacgg tatgcggagc atacagtgca ggtcaacagg gaagcaggcc ggatgcattt
    33121 ttgaatatat ttattttgca aggccggaca gcgtcatgga cggcattagc gtataccagg
    33181 ccagggaaaa ggcaggcagg atactggcgg aggaacagcc tgccgacgcg gatgttgtta
    33241 tcagcgtgcc cgattcgggg actcctgcgg caataggtta ttcgcttcag tcaggaattc
    33301 cctatgcaat aggtttgata aagaacagat atataggaag gaccttcata cagccttccc
    33361 aggttaccag ggaaataggg gtagccttga aactgaatcc tttaaaggcg aatatcgaag
    33421 gaaagcgtgt tgtaatggtt gacgacagca tagtgagagg tactaccagc aggaagattg
    33481 tgcaggccct gaaggctgcg ggggcaaaag aggttcatgt tagggtaagc tccccggtaa
    33541 ttacccattc ctgccatttc ggaatagaaa cttcaaacag ggatgagctt gtgggcgcga
    33601 ccagtgtatt ggaagcaata agggaaaaaa taggagcgga cagccttgga tatctcagct
    33661 ataacggaat cctgcggtcg gtgggaagca cgggggaggg cttctgtacc gcatgcttct
    33721 caggaaggta tcctatggat ataccggaaa attgcagcaa ggatgttttc gagaggaaaa
    33781 aataattttg aaacagaggt gtgggatcct atggatgaaa aattgacata caaggctgcg
    33841 ggagtagatg tggaggcagg ctatgaagca gtgaagctga tgaagcagca tacaaaacgc
    33901 accatgatac ccggagtgct tggagaattg ggcagcttcg gcgggttttt cgagatagac
    33961 agggaaagct ataagaaccc tgtcctggtt tccggaactg acggagtagg aaccaagctt
    34021 cagatcgctt ttatgacagg aaagcatgat acaattggga ttgattgtgt ggcaatgtgt
    34081 gtcaatgacg tagcctgtca cggagcaaaa cccctgttct ttcttgatta catcgggaca
    34141 ggcaagctta tgccgaacat agcggctgat atagtaaagg gcgtgtgcga cggttgtgtt
    34201 gaagcaggct gcgctttgat tgggggagaa accgcagaaa tgccgggctt ctataaggat
    34261 ggggaatatg acctggccgg ttttgccgta ggaatagtgg acaagggcaa tgtgataaac
    34321 ggctccaatg ttaaagaggg agatgtgctg ataggcattg cttccagcgg catacacagc
    34381 aacgggtatt ccctggtaag aaaaatcttt ttcgggataa acaattttga tataaatacc
    34441 agatttgacg aactggaggg cacccttgga gaagaattgc tgaagcctac aaaaatatac
    34501 gtgaggacag tacagtccct gatagtaaaa cacaggataa acggtatagc ccatattacc
    34561 ggcggcggct tcatagaaaa tatccccagg acactgcccg cagggcttaa ggctcagata
    34621 gacctcggaa gctggtatat ccctccggtg ttcaggctga tgcagaggtt gggaagcata
    34681 gaggaaaaag aaatgttcaa caccttcaat atgggcatcg gattggtact ggcagtcgga
    34741 aaacaggacg cggaaggaat tctcggcact cttgaggagc tgggggaaaa agcctgtatc
    34801 ataggcagtg ttgccggagg tgaagggggt atagaatttt gcggaaggta aggatagctg
    34861 ttctcgtatc gggaggcgga acaaacctgc agaccttgat tgatgccgtg gacaaaggtg
    34921 atatcaacgg ggaaatagca gcagtgatat ccgataacga aaatgcatat gccctggaaa
    34981 gggcaaggaa gcacggcatc aaggcaattt atatcaacag gaaactgctt gccgaaaggc
    35041 tcatggcgga acttcaaaag ctggatattg aactggtggt tctggcgggt ttcctttcca
    35101 tactggacag ggagttggta aaagcctatg aagggcggat aataaacata catccttcat
    35161 tgataccgtc tttctgcggc aagggatttt acggggaaag agtacataag gcagctttgg
    35221 aatacggggt aaaagtatcg ggggctaccg tccactttgt ggatgaaggc accgacagcg
    35281 gccctataat attccaggag gctgtgccgg tttattttga agatacttct gaaactctgg
    35341 ctgcgagagt gcttcaggtg gaacacaggc tgctgccggc tgcagtaggg ctgttttgcg
    35401 aaggaaggct ccgcatagag gggcggaagg ttaaaatatt aaaggaagag gtgtgatgcc
    35461 ggtatgatta agagggcgct tataagcgtt tcggacaaaa ccggaatagt tgatctggcc
    35521 agggaactgg caaagcaagg ggtggagata atctcaacag ggggtactgc caaggctctt
    35581 gaggaggcag gaataaaggt tattgggata tcagatataa caggctatcc cgagtgtctt
    35641 gacggaaggg taaagacact gcatcccaat attcacggcg ggcttctggc aataagggac
    35701 aaggcggagc atatggataa gctccgtgaa ttggggatcg ggactattga catggtggtc
    35761 gtcaatcttt atcccttcag aaaaaccata gaaaaagaag gcacagagct tccggaagct
    35821 atagaaaaca tagacatcgg cggcccttcg atgctcaggg cggcagccaa gaactacaag
    35881 tatgtaacgg ttgttgtgga tcccaaggac tattctaagg tgctttccga aatcaataac
    35941 agcggagata caactgaagc aacaaggttt gagcttgctg ccaaggtttt ttcacatact
    36001 gccagctatg atgctttgat aggcaattat ttatggtcta aggccggatt gccgaagtat
    36061 ccggagacag tgaccatgac ctttgaaaag gttcaggacc tgagatatgg agaaaatcct
    36121 catcaagggg cagcatatta caaggaaatg aaaaaggtca gcggcagtat tgccgcagca
    36181 gtgcagcttc acggcaagga gctttccttt aataatataa acgatgcaaa tgcagcactg
    36241 gaaaccctga aggaattcga cgagcccgca gttgttgcac taaagcatgc aaatccctgc
    36301 ggagtaggtg tcggaagcag catatatgag gcatatatga aagcttatga aggggatccc
    36361 gtatccatat tcggcggtat agtagccctt aacagggaag tggatgaaaa gacggcgtcg
    36421 gaaatcggaa agatatttgt ggaaatagtg attgcaccct cctattcgga agaagccatg
    36481 aatgtcctta ccggcaagaa gaacatcaga ataatgaaac ttgaggatat aagccgcaag
    36541 ccggaggcag acatagatgt gaagaaggta ggaggcggcc tgcttattca ggatgcggat
    36601 tgggcggatt ttaatatggc tgatataaat tatattaccg atagaaaacc taccgagaag
    36661 gaaatgcagg atatgctttt cgcatggaag gtggtaaagc atgtgaagtc caatgccatt
    36721 gtagtggcca aagacggtat gactcttggc ataggacccg gccagaccaa caggatatgg
    36781 tctgcaaata tgagtcttga gcgttccggg gaaaaggcaa aaggagctat aatagcctca
    36841 gatgcattct tccccttcgg ggatgtggtg gaatccgccg caaaggcagg aataacggca
    36901 attatccagc cgggaggctc cttaagggat caggagtcca tagacggatg caacaagcat
    36961 ggaattgcga tgatttttac tggggtgcgg cattttaagc attagaagag gtgataatat
    37021 gaaggtactt gtggtcggaa gcggcggaag ggaacatacc cttgtatgga agatagctca
    37081 gagcaagctg gcggataaag tgtactgcgc gccggggaac ggcggaatca gtgaactggc
    37141 cgaatgcgtg gacataaagg ccgacgatat tgacggactt ttggaatttg ccaaaaccga
    37201 agggatcggg cttacggtgg ttggtcccga ggtgcctttg agcctgggta ttgtagattg
    37261 ctttgaggaa aatggcatga gaatattcgg gccttgccgg aagggcgcaa tgcttgaaag
    37321 cagtaaggcc ttttcaaagg actttatgat aagaaacaaa atacctaccg cagcttataa
    37381 ggtatattat gaagctgaaa aagccaaggc agggcttgat gattttggtt ttcctgttgt
    37441 tatcaaggct gacgggcttg cggcaggaaa gggtgtaata atagcccaga acagagaaga
    37501 agcaatctcc gccattgatg atatgctcat ggtcaggaag tttggcgaag caggctcaaa
    37561 gattgtcatc gaggagtttc ttgttggcaa agaagcttcg atacttgctt ttgttgacgg
    37621 aaaaactgca gtgcccatgg taagcgccca ggattacaaa agggcattgg acaatgacga
    37681 agggctgaac acaggaggaa tgggcgcagt atctcccgca ttctattatg atgaaaaaac
    37741 ggctgctgta gtggaaaggg atataatccg gcgtactgta gatgctttaa aggcagaagg
    37801 catctgttac aaaggagtat tatacttcgg aatcatgata accgagaagg gccccaaggt
    37861 attggaatac aacgccaggt tcggggatcc cgagacagag gtggtgcttc tgaggcttga
    37921 gtcggacttg gttgaaataa tgaattcggt aatagacgaa aagcttgaag ctcagaaaat
    37981 ccaatggaaa gatgagcagg cagtctgtgt tgtaatggca tccggcgggt atccggagca
    38041 ttacgaaaag ggctatgaaa taaaaggcct ggatgaagcc gctgaaattg acggagtaac
    38101 ggttttccat gcgggaacaa aaaaggcttc cggaaaaact gttactgccg gaggccgggt
    38161 gcttgtaata agcgctgctg caaaaaatta tgaagaagca agagataaag catatagcgc
    38221 aattagcagt atagcttttg aaaaagccca ttacaggaaa gacataggga aaaaagatta
    38281 aatcattgaa cttattgcaa agtcccgtaa ggggctttga gactgatgaa aaacctccga
    38341 tataagaaca gcaatgtata gtctcgcata ggtatacaaa aattgatggc tctaattcca
    38401 ctcaactaag aaaaacctaa cgctgtcaaa ggtcgtccgt gaccgtgaca gcttgctcgg
    38461 cctccatgcc tcgcatacgg ttgtttctaa gtcgagcgag atttgagcca ttaacaattt
    38521 ttgcataagc atgctctgac tatacattgc tgttcctatt ctgagatttg tctgcaaact
    38581 gaaagtcctg aaaagggctt tattttttac tataaagatt tttttccgcc ggtattttgt
    38641 gattaataat acacgcttaa gtgacatata tcacagctaa gttattttaa cggggatata
    38701 ataaaaccaa agaaaaaatt tggaggtaaa taaaatgata agaaaaattg ttaagattga
    38761 tgaagataaa tgcaacggct gcgggctttg tgtgcccaac tgcgctgaag gtgccataaa
    38821 gataatagac ggaaaagcaa ggctcatagc agataatctt tgcgacggcc tgggtgcatg
    38881 tcttggagaa tgtccccgcg gtgcaatcac aattactgaa agagaagcgg atgaatttga
    38941 cgaaacagct gtacatgagc atcttgacag gaaggaagca aaacctgaag tgaagcagga
    39001 agcccattcc tgcagcgggc atgcacatat tggcggcgga tgcccgggaa gcatggcaag
    39061 ggaaataaaa ttacgcgaga atcccgcaga agcagcagca gtgacttccg gagatatcga
    39121 agtaaagatt aagtcacaac tgactcagtg gccggtacaa ttgatgctgg ctccccaaaa
    39181 agcaccgtat tttgaaaatg cggacctttt gattactgca gactgtgtgc cctttgctta
    39241 tcccaactat catctggagc tgttgaaggg taagaaggtt gtagtgggat gccccaagct
    39301 ggacgatata ggctattata tggagaagct tacagggata ataagaaata atgatataaa
    39361 aagcgtaaca gtgacattta tggaggtccc ctgctgcggc ggaattgtaa gggcggctga
    39421 aactgccctg gagatgtcag gcaagaatat tccccttcac aagatcagaa taaaaatcaa
    39481 cggagaagct gagaagatca gctgaatccg ttaaaataca ggggcggcca tcatcggccg
    39541 ccctgttatg caatatattt ttatttatat atgataatac tattgatagg attgaatgaa
    39601 gcatatataa taaaactata acttaatact gatcaggtgc cggtttgttt atgaataatc
    39661 tgccggctaa aagggaagtc gggtgaaaat cccgcgcggt cccgccgctg taaaagagga
    39721 gttctcctta agatgtcact gagtagattg ggaaggcaag gagaatgatg atgcttgagc
    39781 cagaagacct acctggttta taacactaaa tactctacga gagatagggg ggtgttctta
    39841 tatgcaggat gattatagtc attttgtcac ataacctctt aactatttag ttaagaggtt
    39901 tatcttttta ttaagaaaga aagcatggag gttgtatttg tatgggcaag gcgtattcat
    39961 acaggcgtgt atcagctatt atgctgataa tgatatttct aacggtaatt gctcctgttg
    40021 atttgtatgg aactgattat acaggagtgc ttgacgatgt tgttaattat tataagtcag
    40081 ataagcccgg ccttgattca tggtgggagc ttgtggcgtt aaaaggtacg ggacagaacc
    40141 tgagtgatga cagctggata cttcctcagt gggaaagcga ggatttgcct gccgcagctg
    40201 caaatccggc agattattca ggatttatac tggggcttat ggcaatggat gaaaatccgc
    40261 aaaatgccct tggaggcagg aacctgacgg aggaattggc aggcaggcag gaggagggcg
    40321 gcgcttttgg caatggtgca attaatatgc atatatggtc aatggttgcg ttagatgcag
    40381 cagaggagaa ttatgacagg gaaagcgccg ttgaatggtt actgagcaat cagaaggccg
    40441 atgggggttt tgctctgtcc ggcatcaacg gcgatcctga tatgacagga atggcgctgc
    40501 tggcattatc tccatatata aatcaggctg atgtaagcag tgctgtatct caggcagttg
    40561 aatatctgga aaatgcccag tgcgaatcag gaggctacag ctcatggggc atcgaaaatt
    40621 ccaacagtgc ggcgatggtt atttcggggc ttgtttctgc cggccagggt gtgagcagct
    40681 ccattcttga tgcgctgctg gaatataggc tgcctgacaa tacattcagt tatcagaaag
    40741 gcggcgctac aaatttcctt tctaccgcac agtcccttat agcactggga gacataattg
    40801 agaataagag tatattccaa agattgagca tttctcagca gcccacggat ggagagaaag
    40861 tcaggattcg ggtagaaggc agcagctata gcatggtaaa taaggaaata acagtaacct
    40921 ctgcagtgta tgcggtagat gccttgaagc aggtattgga ttccgaaaat atcacctatg
    40981 atatacaaga cggcgtgtgg ggacagtata ttaatggcat tgacggagaa accgcaggaa
    41041 agttcggtgg ttatgacggt tggttatatc tggttaacgg agaaatggca tcggtgggtg
    41101 taggtgaata taaaatcgaa gacggagatg aactggtatt ttattatggc atgtatccac
    41161 cggatacttt gattccccaa gtggatatac aacctgtaaa tcctaaggta ggagacaatt
    41221 taacgattac tgtcacttca agctattatg actggaatac cggcagtacg gttgagctta
    41281 aagttcaaaa tgctgaaata agcttcaacg gcaagaccta tgccacggat gccaatgggc
    41341 aggcaattgt gccaagtccg gctgataagg gtacatacac cttgaaagta agtaaggata
    41401 acgatggcag ttatcccagc atcgtccgca tcccaggtat taatgttata tatagggcga
    41461 atggtaatgg cggcggaggc agcggcagcg gaggcagcgg ttccggcagc ggcggctctg
    41521 cagaaactgc aacattatca gtggaaggtg acagccgtaa aggtacgata ttgcctgctg
    41581 tccaagtatt attgcgggat ggggatactg cttacagcat acttgcaaga aaaatgggaa
    41641 gtaaggtaaa ggctaaaggc tccggctcaa acatatatgt ctatgagatt gacggcttgg
    41701 ctgagtttga caggggtccg tcaagcggct ggatgtttga ggtcaacggg agaaatcctg
    41761 atattagcgc ttctgcctat gtattgaagg ataaggatgt tgtcaattgg tattattcat
    41821 ccgattccaa tgagagcttg ggtgcaaccg gtcagacagg aactgcacag tcagggaata
    41881 gcagcgcgga aatcagcagc acgtcaaatg aggcaataaa gaaagcattg tcagagaata
    41941 ttcagaattc agctaataaa atactcacac aggaaaacat ttcggattgg tctgttttcg
    42001 cactgcaaag ggcaggccgg agcatacccg aaaaatatat tgaatcccta aaggtataca
    42061 ttaatgaaca tgcagcgggt tccggaagtg caaccgatat tgcaaggatg gttctggctg
    42121 caagtgcagc aggaatgaat cctgcgaaca tagacggaaa taatctgatt gaaaagctgt
    42181 tcagcatgga gaaaatcgac aggcagggag tcacagggcc ggtttttgcg ctgatagcat
    42241 tggacagcgg accttacagt attccgtcag gcgcaaggtg gacgagagat aagcttgttt
    42301 cactgatact tgaataccag aatgaagacg gaggcttctg ccttgcaaaa gatgagaaaa
    42361 gcgacataga tttgacagct atggcactgc aatcactgtc cggttacagc agcgaaagcg
    42421 ctgcttcatc cgtagagaaa gctattgatt ggttatctaa cctgaaaccg gaagaggagc
    42481 aaaccagtga gagcatttca cagattatca tagctcttac atctcttggt aaggatccgg
    42541 gaagcataca gttcggcatg tatgatgggg atttgctgac ctgcctgctg aaatataaaa
    42601 aggaggacgg cggattttca catacattgg atggagacag tgatgaaatg gccacggaac
    42661 aggctttaat ggccatcact tcatacagca tgtatgttaa cggttcaggc aagctttatt
    42721 ccctgcaggt tcctaatggg gcatattcag accttcatgc gatttccgaa tgggcactgc
    42781 cgtatgttgc caaggcctct gagcagggac tgatggaagg gataaaaaca gatgcttcag
    42841 ggcaaaggtt tgagcccagg aggaacattt caagggcaga gcttactgcg ctgcttttaa
    42901 ggattttggg agaagaaccg tcaaagagct ttacgccggt atacaatgac ataaaagagg
    42961 gttcatggta ttcaggatac gttctgaggg ccagagaaaa gggtatcatt aacggtgtta
    43021 cagaagtcac cttcgacccc aacaaaccgg ttacaaggca ggagatggct gtcatgacgg
    43081 ccagagcttt taatcttggt gaaaaagcat caggcagcag gataaatgac ttggacattg
    43141 cttcggaatg ggcaaggcag gctattgagg ctgtttacga taacggtatt atgatggggg
    43201 aaagtaatat gttcaacccg aagggttatg tcagccgtga aatggcagca gctgtcggag
    43261 taagactcta taatatgaag caataataat attaagcctc ctgcctgtaa aaatacaggc
    43321 aggagcaaaa tccattattt aatgcgggag agtaatatga gaagattcac atttttatta
    43381 tatgcagtac ttctgataat tttatcaggg tgcggcaatg cagcagatgc ggacattccg
    43441 aattcggaga agatttcaaa gccggtattg tctgatgaaa gcaattgcat aaagggtaca
    43501 aaaaaagaag atatcccgga tggtaaaaca agtattaaac cggagagtaa atcggaaagt
    43561 aaaaataatg aagagcatgt agtaaatcaa gaagcagcaa ctgccaaggt acatatttcc
    43621 attacgggac ataaggatgt ggggataatt cttgcagata cggcagtaaa tattgatgag
    43681 ggggatacgg taattgatgt tttgaaaaga actgcagaac taaacagtat acagctggaa
    43741 tgcagcggaa tcaaagccat ggcatatgta aaaggcattg ggaacaccta tgaattcgat
    43801 tacggtccca agagcggctg gatttacaga gtaaacgggg aaatatccaa tgtaggtgcg
    43861 ggcaaatatg aagtgaaaga cggagattat atcgaatggc tgtatacggt ggatttagga
    43921 agggagtttg atacggaatc aggaggagct caggaatgaa tgcaggattt tcgtcggtac
    43981 atcccggagt atgctttaca tattataccg taataatggc ttttgcaatg attttgtacc
    44041 atcctgctta cctggcctca atgcccatag ctttgataac ctggctattg ctgcatgaaa
    44101 agggcagaag cctgtccggt tcatggcatt attacctgct gacaggtatt gcggtactga
    44161 taataaatcc tctcataaac cacagagggg agagggttct gttttttctc ggtgaacagg
    44221 ctgtgacctt tgaagctgtt gtatacggat tcactatgat gctgtcgatg ctgacaatac
    44281 ttattgcatt tatttgtttc cgtcaggcag tggataatca taaattcctg tatcttttct
    44341 catccatcct tcccaaaaca gcattcctga taatgatgtc aatgggcttt gtccctttgc
    44401 ttactgacag gatgaagcag atatccattg ttcagaaagc cagggggctg gatatgacgg
    44461 aaggaactgc cgtaaagagg gcaaaggacg gtatgcaaat attgaagatt cttatttcct
    44521 ggtctcttga aggagcactg caaactgccg attcgatgaa agggagaggc tatggaattg
    44581 caaaaaggag cagctatatg aaatacagga tagatggcag ggattggctg attatggcat
    44641 caattgcaat actatcggct atcacttcca ttggtttata ttttggatat ggaaagcttg
    44701 tgatttttcc gtccttggaa atcataagca tggatattac cggcattgtt ttttattctg
    44761 ccttctgttt gctgttgctt ataccaataa ttgttgaggg aagggaaatc ttaatatggc
    44821 actgttacaa ataaacaact ttaattttac ttatgcggga gatacaaaaa aggttctttc
    44881 aggaataaat cttaccgtcg ggcaaggaga gtttattgta ttatgcggac catcaggttc
    44941 agggaaaacg acacttctcc gtaatataaa aagagagata agtcctgcgg gcattaaaac
    45001 cggagatata ctgtacaaag gtatggcctt agatgaatta aaggctgagg taagtgcaaa
    45061 agagatagga atggtgttcc aggaaccgga cagccagatt gtcatggata cggtgattca
    45121 tgagctggcc ttttcccttg agaattttgg gtatgcacct gattttataa ataagaagct
    45181 atccgaaatg gccgcttttt tcggattgac tgaatatctg cataagccgg tgcaagaact
    45241 gtcaggaggc cagaagcagg taataaacat ctgttctgtc atgctaacag agccgaagct
    45301 gcttttattg gatgagccta catctcagct tgatccggta gcagcccgtg aacttataag
    45361 cttgattcat cagataaacc gggaattttc tgttactgta ataattagcg aacacaggct
    45421 ggaagagctt ttttcaatag cagaccgggt tgtttatatg gatgggagta ttattaagta
    45481 ttgcaattct ccggatgggg tatcaaagga gattatggaa gccaaagacg aatatgcagt
    45541 taaatctatc cctccaatag tacgtttgtt tttcagcctt tcaaaggaga aaggcaaagg
    45601 cccagggtgc ggcattccca tgagcgtaag agaaggaaag cagtttatgc aaaatatcag
    45661 atgcagtgtt aacgggttga ctgatatgga aatcaagaag gattgggttg aagagatcat
    45721 taactgtaag gatatcggct ttagatatgc cagggaagag cccatggttc tagagaaact
    45781 gacacttaaa ataatgaaag gggaattcat ggctatactg ggcggcaacg ggtcaggcaa
    45841 aagcacgctg ctgctggcga tggccgggct gataaccccg cagcagggca gcatctgtgt
    45901 cagaaagaag aatatcacct atatgaaaca gtctgaaaga aacggtataa taggctatgt
    45961 tgcgcaaaat cctttgcttc atttcacatt ggatacagta aaggaggagc ttgaatttgc
    46021 attgcagaac tcattaaaaa ccggaagcaa agcaaaaatg gaagagctta ttgaactatt
    46081 cggcttgaag gatttaacgg acaggcatcc ctatgatttg agcggaggcc agcagcaaaa
    46141 tctggctgtc gcatgtgctt tgattcatga accggagata ctgttgattg atgaacctac
    46201 caaggggctt gacagcatat caaaggaaaa acttgcagaa ttgttaaaaa gccttaaggg
    46261 taaaggtact tccgtagtta tggcaaccca cgatattgag tttgcagcac actatgctga
    46321 aaaatgcgca atgctgtttg acggttctat atcagattgc gctgaacctt caaggttttt
    46381 cagcggcaat tacttttata ctacttcgat taaccgggcg gtaagggatt atctgcctca
    46441 cgcaataaca tatgaggatg tgataagggc atgcctatga taagattcga gacaaaaaag
    46501 caggacccgc gggaattggt tctgatttcg atgcttgcag caatagcggc aatcagcaga
    46561 atattgttcg cacctctgcc gaattttaaa ccaatgtctt ttgttattat tgttaccggc
    46621 attgtattcg gcccggaatc cggcttcatg gtgggatctg tatcggcaat cctgtcaaat
    46681 atttttttcg gccagggtcc gtggacttta ctgcagatgc ttgcctgggg attgatgggg
    46741 cttacggccg gacttttaag aaatacggtc tggattaaaa gcaggtatgg aatattaatg
    46801 tttggattct cctggggatt catattcggt tggataatgg atgcatggta catcatagga
    46861 ttcataaggc cgttgagcct gatgacaatt attggaggcc tttctgctag cttttatttt
    46921 gatttgatgc atgcgcttgc aaatactttt ttcctgggaa ttttttcaaa gagctggata
    46981 aagctgttta atcgcataaa gataaaatac ggtttactgg aagactctta gatatataat
    47041 gctatatcgg aggaacctca ttttacgaat tttatcatga aaatacctat tccaattagt
    47101 atcagattgc ccgcctttag cccggaatcg ctctcaagtg cgtttatatg gggtaaagcc
    47161 ttgaagtatc tgattggagt cattctgatg tttgagtccg atactttaat ataaaccgca
    47221 tcacgtatat cataagtaaa tccgtctatt ttctctccgt caggaagcgt gaaagcatag
    47281 tttacaacat aggtgtacct gttgggtatt gcttcgcctc tttcgccacc ctgccttcga
    47341 atgccggtta ttacagcagt gtccttttca ccggcaaacc ccagggaaat ccgcagcaag
    47401 cccaaggcaa tcatcataac tgcaataagc attactaata aaggcccgat tctttttttc
    47461 agattagttc tgaacatacg gctattcccc atttgcattg aatttgctta atataatgtc
    47521 actaaccaga tgaaagttgt cctcattaca gaacaaagcg attttttccg caaaaccccc
    47581 aataacaact atagatctcg atttgggata tacttttact tttcttatgc ttttccatgc
    47641 tataaatacc gattgccttg tttgagccag caatccggca cctgctgcag agggcctttt
    47701 tgaaaacagc ccgatgataa tgagcaatga gtttacaata aggtttttct tcgcttgctt
    47761 cctttgagtg tagttgagga ttccgttctt atcaattaga taaccggcag agtatctgcc
    47821 cccatataca accatgataa acagaaaacc cgcaataaat aatagaccga ttaaggtcag
    47881 gggatatttt attccgtcag cggaaacgtc gcctcctgaa gcaataaaga gaatgaggat
    47941 aagcagcccg aagggaattc ccagcgcaat tcccagatcc tttaaaatcg cattattctt
    48001 gaatatgggc acatcaattt cccaatagaa tttggtttgt ttatattcat ccataattat
    48061 atactccgtt tatctagtat aaattggtaa tgcaataacg gcaataactg cagcagatgg
    48121 catacagtaa tacccatttt ttcagccggc caattccgga atgatgcctg tctcacggcg
    48181 gaatttctca atgatatccc ggagttcagg ttcattaata ttgctatgca gcagtcttat
    48241 agttttatac ttttctccct taccataggc caataggata taatcatgtc cgacttcaac
    48301 ttttttcagg ctgattcctt cggtattgaa tttaattatc tttgaatcaa gcagaattcc
    48361 aaaacaggat acaaagattt caatagactt attcattttg gattcggatt ctgtttcagc
    48421 cgcttcaata tgaatgattt tattttctat tatcagcctt gcaaaggcat accttctgta
    48481 ttcattcaga gagaatacca agagcaggat agttgtcaga atcgagaata caacaactat
    48541 atggaaatac ccggcaaaga gcgtaaagga aataagaatc ccaaaactgc tcagcatcat
    48601 ataaatcata agtttacgct taattgtatt caaagcgcta tcccccttta ggaataaagc
    48661 tcctcccctt tttacggaga ggagctgata ttatccatac tcaggattat tcttcgtata
    48721 ctatgactgc ttccttggtg ctgttaatcc agtccacttt gcagttgagg ttctctgctg
    48781 caaatcttac cggtacaaag gttcttccgt tcttagatac gggggctaca tccattttct
    48841 ggctgactcc gtttattttt atatcagttt tgtccaacca catttcaacc acatttcctc
    48901 tggctgttag tgttatctta cgtgtgtctt gctcccatcc tatcgtgcct cccatggctt
    48961 ccacgacggc acggattggt accattgttc ttcctgcaat ggtcataggt gaggtgctgc
    49021 gtcccgggtc cacttcctgg attactccgt ccacactcat attcgggttg tcaagcttga
    49081 gcatgatgaa gtgcttaaag cttccgggct tgtagacctg gctgcctgtt gttactgtat
    49141 atgtggcaat tgcagcaccc agtttttcat ctattccttc gaagggtttt gcttctgcga
    49201 gtaccggctt tacagtgtaa tagtaagtgg tgttggcttc tatatttaca tcagcataag
    49261 aggtgccggt tatataaaag tcagttactg atatgccaag gtcacttttg gaggttgacc
    49321 tgaacagcct gtatcccaat ccgtcagcag gctgccacat tatccttgat ccggattcaa
    49381 atgtttctgc attgcttcct gccgaattgg cctgagttgt tgtaattaca ggtgcaggag
    49441 atgttgctgc cggttttggt gcgggaataa cctcagtaat acgcatgctg ccatcccaat
    49501 tactccagct tccttctgaa ccgtagcgcc atctgccggt aaagcttttc ccgtcggcgg
    49561 acattgtaag ctccatgtca ccggcatcgg acggtggtga ataggatggg gactcggacc
    49621 aggtaccggt aaagacattt ccggatattg tgccgttgat ttttccgctg tcgtgggtat
    49681 aggtacctgt aacagaagca ccgttttggg ttattaacat tttaccccag ttgctgtccc
    49741 attcccctgc ccaggagtag gtaccaagat cggtagttcc tgatgaaatg ggagcagcat
    49801 ttgctgatga ggtttgcttc cattcatagg tataccttac acctaccgaa cccggtgagc
    49861 ctgcataaac tcccacatag agggaatgct tatctccggg gcggccttct cctatgaagt
    49921 tcagggtcat atctgccgta aaggattgtt gtgcctgcgc atcttgattg tatcctattc
    49981 ccggatactt tgccgtggta ccgtcagcat aggttatttt agcaattacc ttgctggctg
    50041 tggcatatcc gatatctata tcttccatgt ccatatggaa gtaaggattg aagccgatgg
    50101 agtaattccc gttcctgttg cttataacat tctgttcaag ctttaaagtc actgtctggt
    50161 ttggcattat ggtgcttgga ggattgctcc aggtatatat tgcgtgcata tctgtcgggt
    50221 tgtcatagtc ctttgatgca tatataccta agccgtaagc ccatgcatac tgcaattcta
    50281 tagtgttgcc tttaaggctg ccgtaaaagc tatctgcttc aggtaccgga tattcgtgag
    50341 tatccaccag cacccatgca tatccttcct ctgctgccga aacaaccgtt gtaaatcccg
    50401 cagccatact aaagagcagc gcaaggacgg tgactattga tagaaacttc ttcataatat
    50461 aactcctttc cgatatgtaa atattaagta ttcatctatc ccaatatatc tgatatactc
    50521 aaaaaagtcg tcacccgaaa tgggtgatta cgttgattga agtaaaaaat ccctcatata
    50581 taataatgag agatatagaa tgcgtcatta attgtattgc agattagtgg gtttcctttc
    50641 ccgtcagcag ctttataagc tccgttttgc tctgaatgtt gaatttggag tagatgtttt
    50701 ttatatgtgt ttttacagtg ttttcactga gacaaagttc atcggcaatc atcttgtagg
    50761 ttcggccttt gagaagcagg gctgctattt cgctttcccg ctcggtaagc cggccgataa
    50821 ctgtaaaatt ctcaattgct tttttctgct tcgatggttc aatttcgctc aaggccgtca
    50881 gaaaaatatg atttttcaag aggccggaaa gatgcctgtg cagcagcgga agcagtatga
    50941 ggactataaa tactaccaca agcgccaata cggatgagtc aatgcttcct gtatccgaat
    51001 ctgcaatagt gcttccaaac atcccgccta tcaatactcc gaaaacattt gcagaaagcc
    51061 cgatgccaag gatttttgcc ggattatggt gataatccag catttctccc agaatgctcc
    51121 accagaacag gtcatatact ccgcaggccc ccagcatcag ggtatctatg ataagatagc
    51181 ttgcggcaga acggtcaagt atcataaagc ccacagatga aaggcctatc attgcaatgg
    51241 caacgtagag tatgtagctt ctgttggcct tgccggaaag gtttctgaca atatataagg
    51301 ctgctatata gggtaaggcc caataccagc tgactagcca ctcatgatgg gcaaaggcgg
    51361 gattgatgac ctgatacatc aagccggaat taatggtgat tatgacgata aacaagcata
    51421 aaaaggccaa aggctttata ggactgacag cctttatagg ctgtctggta tgcctttctt
    51481 cagctgtatt ctgatccaca gccaatttag tatgaaaaaa caatgatatt gaaagcatga
    51541 gtatggacag tgcaagcccc aaataagaag atataaggac ggcagcagta tttataataa
    51601 ccatgaggat atttgaatag attaggacat cggctatgat tttaattcgt tcataggaga
    51661 aagcgagcat tttgaagtag aagccccagg ctgccacgca agcaccggac aggaatgaca
    51721 atatcagaag cgatatatgc cacattattg aaggaataaa gaaaaaaact acagtaccga
    51781 gtatacaaac aggaatggaa aaaagcatta gaccctttgc ggcttttgtt gattttataa
    51841 aaaatccgca ggcaaacaga ccgataaaat gagctgcaat tgctccgaag gtcagctcat
    51901 ttacgttaac attattaata cttgcaattg taagcagtac ctgtcccccg aagaggaagg
    51961 acaggagcca tgaagaaaag gatgaaaagg caataattgt tttactgcgc tcatctctca
    52021 acaaaaatgg tacccccggt ctttatataa attaatgctt tatggattaa ttatactttg
    52081 aaatgtgcat tattccaaga gcagcatcag aaaattatat agaacacagg ctgaaatttt
    52141 ttgctgtgtt ctatatggat ttttatttcc tattttgaat tcttgttttt attattaata
    52201 atctcctcat gattcatata agaatttacc agctcctcag cctgggcttc ttcattgtcc
    52261 ggattataat tttcggggaa ccagtaggag ttatatttac gttctattat gggatacttt
    52321 tccccgttgt acgtctcatc tattatattc atggcttcac ctcatcctta tccttctctt
    52381 taatgtcttc attctcaagg gcaatatttt ctatcccgtt atatttttta tgtgaatcat
    52441 gatttatttt ggccatgact atttcatagt ccgtaattgg ttcctccgca gcaggagtca
    52501 ggacgttcaa ttttttattt atttttgttg acataaaatt tcacctcgtt ttatttttag
    52561 ctatttttat ttttgtattc attgaaaaat tgcaaagcgt cattgaaacg ttgcggttgt
    52621 tcttctcttt atcagaagga tgctttataa tagtatgtat caaaagaaaa ctaccggagg
    52681 tactgatgaa ttatttggag aacctgaaca aagagcagcg ggaagcagtg ctgcacaccg
    52741 aagggccgct tctgatactt gcgggggcag gcagcggcaa gacaagagta cttacctaca
    52801 ggatagccta tttgatagat gaaaagggca tatatccttc gaatatactt gcaataacct
    52861 tcaccaacaa agcggccagg gaaatgaagg agagagttca cagacttata ggcaataccg
    52921 ataacatgtg gatttccacc tttcattcgg cttgcgtcag aatgctgaga aaaaatgtag
    52981 aaagcctcaa gggttataag aagaactttg tgatatatga tacgaaggac caggaaactc
    53041 tgataaagga atgcctcaag gaactgaatc tcaatgagaa gaattttccc ttcagggttg
    53101 tatctgccga aatatccgca gcgaaggaca ggctgatacc cccccgggag ttctatgata
    53161 agaacatgca tgatatcaga agacggaagc ttgcggatat atacatgctg taccagaaaa
    53221 agctggagaa aaacaatgcc ctggattttg acgatataat cagcaagacg gtggagcttt
    53281 taaagctaaa tccggatata ttggaatatt atcagaacaa gttcagatac attttggtgg
    53341 atgaatacca ggacacaaac ttcagccagt atacctttat caggcttctt gcggcgcagc
    53401 acaggaatat ctgcgttgta ggggacgatg accagggcat atacagcttc aggggagccg
    53461 atatcggcaa tatactgaac tttgagaagg atttccccgg agctaaagtg gtcaagctgg
    53521 agcaaaacta ccgatccacc gaaacaatac tggaagctgc caatgcggta ataagcaaca
    53581 attacggcag aaagagcaag aagctgtgga ccgacaaggg cacaggccgt tccataatat
    53641 atcactatgc tgtggatgaa agggaagagg caaacttcat aatggacgag gttgaaagga
    53701 tgatagccca ggaaaaccga aaactggggg aatttgcgat attgtaccgt accaatgccc
    53761 agtcccgtgt acttgaggaa acttgtatgt cccacggact gccctataga atagtagggg
    53821 gcttcaagtt ctatgacagg aaggaagtaa aggatataat cgcttatctg agagtcattc
    53881 agaatcccga tgaggatttg agcctgaaaa gaataataaa tgtccccaaa agaggcatag
    53941 ggaattcaac tctggaagcg atagcccaat atgcccggac tacgggagat tccatattcg
    54001 gtgccctgct tgaggttgac aggattgaag gagtaagcac aaaggcaaaa aagggaatca
    54061 aggaatttgt taagattatg agcgatctta tgggcatagc ggagaccgaa atggtatcaa
    54121 taattgtaaa ggaagtgctg gaacgcacgg gatatcttga ccagcttgaa aaggacggag
    54181 atgaagaatc cctaatgaga gcggagaata tcaaggagct gttatcggcg acctttgagt
    54241 ttgaggaatt gaatgaaagt gccttattgc ctgagtttct tgaacaaatg gcccttatgt
    54301 ccgacataga tgcggtggag gaaggcaaag acgctttgat aatgatgacc ttgcacagtg
    54361 caaaaggcct tgaatttccc ttcgtattca tatcgggcat ggaagagggg gtatttccca
    54421 gccagaggtc atacttcgaa gacaatcaga tggaggagga aaggcggctg atgtatgtag
    54481 gtataacccg ggctaaggaa aggctttacc tgacagccgc atttgaacgc acattatatg
    54541 gaaatactac ctttaataca atatcaaaat ttgtacggga gattccgaaa gaattgttag
    54601 taaaagttta agtttttagt ttgtatattt attgcaacgc agccggagct tcaaagtcag
    54661 atacttctaa actcgttccg ggctgcatca aaactcgctt agctcaaaca gttgatgcag
    54721 ctgaccctcc acttcgttaa gaagttctag tgcttttacg caaggctgct tttacaataa
    54781 acatacaaat tcaaaacata taatcttaat tcctgatgta attctaaaat aatagtatat
    54841 caaaccgaag ggaggaatcc gattgggtat agataaactg gaaaaaaggg ctgatgagct
    54901 tattaagctc ataaataggc ataattacaa ctactacgtg cttgacaagc cggaaatcag
    54961 cgattatgaa tatgatttgc tcatgaagga gcttatagag ctggaaaaca cattcccggc
    55021 tttgaagcgg gccgattctc ccacccagag agtaggggga gaggtcctta aggggtttgc
    55081 ggaagtcgtg cacaagaccc cgaagctgag cctgagcaat gtatttgatg aaaacgacat
    55141 aagggatttt gattacaggg taagaaaggc cgtaggaggc gacgtggaat atgttgtcga
    55201 attgaagata gacgggctca cagtggtatt gaactacgag gaaggccggt ttgttctggg
    55261 ggcaacgaga ggtgacggaa taaagggcga ggatattact tcaaatctaa agacggtaaa
    55321 atccataccc ctgctgcttg aagaggaggt tgacctggag gtcaggggag aagttttcat
    55381 gtccaaaaaa gcctttgaag agctgaatgc aaggcgtgaa gagcaggagg agcctttgtt
    55441 tgcaaatccg agaaatgcgt cggcgggctc tctgaggcag ctggatacca aagtgactgc
    55501 aggcaggccc ctggatatat ttatattcaa tcttgaaagc atagagggaa aaaactttga
    55561 tacccatgtg gaaaccttgg aataccttaa ggaattgggc ttcaaggtta gccccttcat
    55621 cagcgtgcac aaaagcagcg aggaaataat agaaaagtgc aaatactggg ctgacaaaag
    55681 aggagagctg ccctttgtaa ttgacggcct tgttataaag gtaaacaacc tcaggcaaag
    55741 agaagccatg ggatatacga caaaaaaccc ccggtgggcg acagcatata agttcccccc
    55801 ggagaccaaa aagaccaaag ttaaggatat tatagtccag gtgggaagga cgggagccat
    55861 aacccctact gccgagctgg agcctgtaag gcttgcaggg tctgttatca gcagggcaac
    55921 ccttcataat gaggattaca taaaggaaaa ggatataaga gtcggagata cggtattaat
    55981 caaaaaagcc ggggatgtga tcccggaggt cgtggaagtg gtcagggagg acagggacgg
    56041 atcccagcag cccttcgaaa tgccgaaaaa atgccccgtt tgccagtcag acaccattag
    56101 ggagcaggga gaggcagtga ccaagtgtac aaacatttca tgccccgccc agcttaaaag
    56161 ggcattattt catttcgtat caagggatgc aatgaacata gaagggttcg ggccccagat
    56221 aatgagcctg ctgatggata aagactttat cagggatgcg gccgatatat acctgttaaa
    56281 gaatcacagg gatgaacttg ttgaaataga aagaatgggt gaaaaatcag tacagaacct
    56341 tttggatgca attgaagctt ccaaggtaaa cccgctgaac aggcttatct atgccctcgg
    56401 aataaggatg atcggacaga gggcgtcgca ggttctggca aatgaatttg aagatatcga
    56461 agccatgttt gatgcggact atgaaagatt gaccggaata tccgaaatag gggataaaat
    56521 ggctgaaagc ataataactt ttttcaagca ggagcagaat ataagattaa taagaaagct
    56581 tcaggaatgc ggtgtgaata caaagggatc aaaaaaggaa ataaaggaaa atgaacattt
    56641 caaaggcaaa acctttgtac ttacaggagg gcttgcaaac tttaccaggg aacaggcgaa
    56701 ggaaataata gaaagctttg ggggaaaagt atcgggaagt gtaagtaata agacagatta
    56761 tgtactggca ggtgaaaatg cgggttcaaa gcttgacaag gcaaatgaac tcaatgtggc
    56821 tgttattgat gaggatacct tcagaagctg ggctgaaata tagaagatta atacaattaa
    56881 agttcataaa aaaatcaaca aaagttcaca aaaaattcac aatactctgg tatattaata
    56941 tttaagatgt atatcaaata tacatcgatt agcaggtttt aggggtatat cttacaacag
    57001 cataatcaag acagtgtttc atctctgtta agtcatggca acggatgaaa cagggtttca
    57061 aagattagtt gttatattag attactattg aatttgaaca cctgctgatt tttttaaaca
    57121 attataggac atttcaagca tatcgaagta tttttactat ttgacgccgg aataacggca
    57181 agaaggtttg cattactaat attttatgtt cataaaatat tttcattgtt gatttaatat
    57241 ttttgttagg ctattaattt acatagatgc ctaaaactaa aataaagggg gatgaattat
    57301 gaaaaagtct ttagttttag tactggcaat gctgatgatc ttgagctttg cattatcagc
    57361 atgcgcaccg aaggctcctg cagaaactcc cggagcagaa actcctgcac cggctgcaga
    57421 accaaaaata ttcagaacca acaatatgag cgagcccggc tcactggatc cggctctggc
    57481 acaaggaact catgaatctt ggatacttga ccatgcattt gaaggcctta tggcaagaga
    57541 tccaagcggt atatctgtac cgggtatggc agaaagctat actttagcag atgatgaggt
    57601 tacttataca ttcaagcttc gcgatggaat cacctggtca aatggtgacc ctgttaccgc
    57661 atatgatttt gaatttgcat ggaaacgtgc attggatccg gatttggcag cagattatgc
    57721 attccaactc tattatctta aaggcgggga agcttacaat acaggtgcag gctctgtcaa
    57781 tgacgtagga gtaaaagcta ttgatgataa aactttagaa gttgttcttg caacacctac
    57841 tgcttacttc ttggagttaa catccttcta tacttactat ccggttaaca agaaggttgt
    57901 agaagctaac gccgattggg caaaaagtgc tgatactcat gtgtcaaacg gacctttcaa
    57961 attaacagga tgggaacaca atgctacaat tacgttggct aaaagtgaaa actactacaa
    58021 taaagaagcc attaagttag atggtattga gcttgcaatt attgcagatg aaaataccgc
    58081 ttggcagaaa tatttaggcg gagaatttga tttccttact cctctgccac aagcagttac
    58141 tgcacagtta aaagctgaag gcaatcctga actggtaatc ggcggagaag tcggaacata
    58201 ttattataac cttaataata aattaaaacc ctttgacaat gctaaaataa gaaaagcttt
    58261 gtccttgact ttggacagac agacaatttg cgataagatt gcccagggtg gacagcttcc
    58321 ggcagaggga gttgttcctt tcggaatgct tgatgaaaac aacaaggaat acagggacgg
    58381 cgcaggtaaa ttagtagagt ataatgtgga agaaggaaag aaactgtttg ccgaaggtct
    58441 ggctgaaaca ggcttcaagg ttgaagattt cagcaaattc acccttcttt acaatacctc
    58501 tgaagctcat aagataatag ctcaggcagc tcaggaaatg tggagaacaa atcttggtat
    58561 cgaacttaca ctggaaaacg ttgacttcca ggttaagctt gacagagaaa aagccggaga
    58621 ttttcagatt tcacgtgccg gatggatagg cgactatatt gacccgatga ctttcatgga
    58681 cctgtggtta tccgaaggtg cctttaacga tgcaaaatac aacaatccgg aatatgacag
    58741 attgattaaa gaatcaaaag caactacaga tcagaaggtt cgtatggaaa acatgcgtaa
    58801 agcagagcag atgctaatgg aagctatgcc ggtagttccg gtatacttct atactcagcc
    58861 atatgctcag aaaccatatg taaccggcgt atttaaaccg cttaacaggt atcctatatt
    58921 cacatttgca gatatggata tgagcaaaag ataaaataat ctaaattttt atagaagatc
    58981 gatgggctgt ttatatacag ccctcggtgt tctttttatt aatggcaaag attaatggta
    59041 aaggaggtaa agaatgaaaa agtatttaat aaaaagggtt ctgatgtctg ttgttacaat
    59101 ttgggcgatc attaccataa cctttgtatt gatgcattct gttccgggta atcctttcag
    59161 acaagaaaat aagatgccta aggtagtata cgaaaatctt ttgaaaaaat atggattgga
    59221 caaaccatta agtgaacagt acttcatata tttagggaag atagttaagg gcgattttgg
    59281 tgattctatg aaatcaaggg ttgaaacagt aaatgatatg attagaaggg gcttcccggt
    59341 atctgcggta ataggtgctg aggccttatt gatagctata atattcggcc cggcattagg
    59401 ggcgattgcc gccttgaatc agaacaggtg gccggactat ttatctatga taattgctat
    59461 tatcggtatt tctgtaccaa gttttataat gggtacagta tttatacaat ttctggcccg
    59521 aaatatagaa tggctgccta ttggaggatg gggaagtttc aggcattcaa ttttaccatc
    59581 tcttgcctta gcccttatgc ccttggctca gcttgccagg tttatgcgct caagtatgct
    59641 ggaggttatg ggacaggatt atataaatac tgccaaatcc aaaggaatat ccaaagctac
    59701 ggttgtatta aggcatgctg taagaaatgc catactccct gttgtctcag ttttaggtac
    59761 cgttgcaacc aaccttttgg tcggtagctt tgttatagaa aaaatatttg gtatcccggg
    59821 tctcggaatg ttctttatta tttcgatcaa taatagggat taccctttga ttatgggaac
    59881 tacaatcttt tatgccatta ttttaattgt tatgttattg attgtggaca ttgcttatat
    59941 gttcattgat ccaagaataa agcttgctaa ggaggggcga tgatgattac tgaatttgaa
    60001 tattcacctg agctatttga attagcatca aaagaagata gaaatgcaga aaaaatacgt
    60061 cgtcccagca taaaatattg gccggacgta tggcgcagac tgaaattaaa taaattagcc
    60121 atggtaggtt taagtcttat agtttttttt ggtattatgg cgtttgtggg tactcaaatt
    60181 aatggatata catattttga acaaaactat tcattgataa atgtgcaccc tgacagcgtc
    60241 cactggtttg gaactgatga gttaggaaga gatttgttca caagaacctg gtttggtgtc
    60301 agatattcct tattcatagg agttgcggca gcttttattg atttctccat aggagtattg
    60361 tatggaggta ttgcaggtat tactaccaga agaatagatg ctgtaatgat gcgtattgca
    60421 gaggttatat acagtattcc ctacttatta gtggtaatcc ttatatcagt tgtgttttct
    60481 gcagccggcc aaggaagttc aatgtttgtt ttgattttgg cactgtcttt gacaagctgg
    60541 gtacctatgg ctatactcgt ccgtgggcaa gtgcttcaat tgaaagaaag cgagtattca
    60601 ttggcttcag aatccctggg ggctgataag aaatggatat tgaggaagca tattatacca
    60661 aacactcttg gaccaatatt ggttaatatt actttatcaa tacccagagc tatttttgca
    60721 gaagctacct tgggctttat aggtctcgga cttcaagcac ctaaatcaag cttgggaact
    60781 ttggctaaca gcggattagc gggccttccg gtggggaatt cttatcaatt attcataccg
    60841 gcgatattta tttcactgat tatgtttgct tttaatgtct taggcgatgg acttcgtgat
    60901 gctttggacc caagactgcg aaaatagaga gaggtggaaa tatggaaaat gtactagaag
    60961 taaaaaattt aaagatatcc ttccagacct tttttggcga agttgaagct gttcgaggta
    61021 tcacgttcag tgtaggcaag aaagaaacct tagctattgt tggagaatca ggctgtggga
    61081 agtctgtaac agcaaatggt attatgcagc ttctgcctaa gcctccggca tttttcaagg
    61141 gaggagaaat actattcaac ggagaagacc ttttgaagaa aacggaaaag caaatgcagg
    61201 caataagagg aaataaaata tcaatggtat tccaggaccc catgacctct ttgaatccaa
    61261 caatgaaaat cggatatcag atagttgaag gcttagtgaa gcataataag atgagtcgtg
    61321 aagaagctaa aaagaaatct attgaaatgc ttgaactggt agcagtacct cagccggaaa
    61381 aaagaataaa ccaatatcct catgagtttt ccggaggtat gcgccagaga gtaatgattg
    61441 cattggctat ggtgtgcaat cctcaattgc tgattgccga tgagcctacc acagctttgg
    61501 atgttacggt tcaggcccag attctggaac tgatgaagaa gcttcaggat cagatgggga
    61561 tgtcaataat ccttattaca cacgatttgg gaatagtggc cgacatgagc gacagggtcg
    61621 ttgttatgta tgcggggcag atattggagg taggtttgac tgatgagata tttgctgcgc
    61681 cgaagcatcc ctacacaaga aaattgttag cctcggtacc tcgtttggat atgagcaggg
    61741 aggaaccgct ttattcaata gacggaacac cgccggattt gtatgttccg cccaagggct
    61801 gttctttcta tgaccgctgc agtgaggcca tgaagatttg caagaataat atgccggaat
    61861 ttgatgagca cagtgcaact catcaaagcc gctgctggtt gaaccatccc atggcacaaa
    61921 aaacaaattc aacggatggg agggaagtat gatgacagaa cctttaatct ctgcaagaaa
    61981 tataaaaaag tattttgatg tgaagggcgg aaagctaaaa gctgtggatg gcatcagctt
    62041 tgatattttt ccccgggaga cctttggttt ggttggagag tccggttgcg gaaaatcaac
    62101 tgccggcagg actatagtgc gtctgtacga tccaacggag ggagagctta tattcaacgg
    62161 acagaacata tataagcttt caagaaagga tatgcaggag atcagaaggg attttcagat
    62221 gatattccag gatccctatg cttcccttaa tccgagaatg acagttgaag atattgtagc
    62281 tgaacctctt gacatacatc agacctataa gaatagagca gaacgtaccg aaagagtaat
    62341 agagctttta agacttgttg gtttaaatga cgagcatgcc atgcgttttc ctcatgaatt
    62401 ctcaggaggg cagcgtcaaa gaataggcat agcaagggca ttggctctga atccgaaatt
    62461 tatcgtctgt gatgagccta tttccgcatt ggacgtttca atacaagccc aggtggttaa
    62521 tcttttgaaa gaactgcagg acgggctggg cctgacttat atgttcatag ctcacgattt
    62581 gtcaatggtc agatatatat ccgacagggt tgcggttatg tatcttgggc atatgatgga
    62641 gcttgcagag tcggatgaac tgtactccaa tcctgttcat ccctacacca aggctttgct
    62701 gtcggctata cccataccga atcctgaaat acaaaggaac aagaaaagga taatgctgga
    62761 gggggacgta ccaagcccga taaaccccag agaaggatgc aggtttgttg acaggtgcca
    62821 atatgccata ggacgatgcc gggaagaaac acccgtattt gaagagaagg cagcaaatca
    62881 ttttgcggca tgccatgttg tatagttgat aattggataa tggcgctgtc gaatattgcc
    62941 cgggcaatat tcgacagcgc cattactcat tattcacata gttttttcag atttgtgaaa
    63001 actaatacca caaattattt aggagagcaa tgccatgacc cttgaagatt atttggagga
    63061 aggctgtgta tatcatgtgg tcagtattat tgacttgaaa aagaccctgc atgaagggat
    63121 cagatacgat gataagaata cttatgcctg taaatatact gacttccata gctttttcga
    63181 cctctacaag cccaaaggaa ttccggactg ggtagagagg aggaaggcca tatttgccag
    63241 tatcgggttc aaggaaggtc ataaatggca ctctcacagc gccatattga ggataaggat
    63301 tttaaaagac agatgctgga tatgtaatga aaatctggca aatttcatat atgagccctt
    63361 tatacttcag cacatggatg gattcgaggg cacaaaagaa ttcatgaagg ctaacggaaa
    63421 agcatttgcg gggaaatatt ggcagaactc attgagcttc aacgataacc ttatcaaaag
    63481 gtatgacaaa agggaaggct acgatgccga agtactggta atgcatccca tacctccccg
    63541 gaatattgaa tgcctctata tagtatcaga tcaccggata atgagctatc aggcgtggaa
    63601 ggaatatttc acaccgggaa gccctcatgc tgaagttcaa tacaacagat atttgcagcc
    63661 tgacaatatg tcttcggcct ggaagtaccc cggctcacaa tgatattttc ttttattcag
    63721 cttgacttca aaatactcag aaaagacagg agctgagcgt ttttccgtgg agcccaggtt
    63781 atgaagctcc agcttggtat cctgcagtat agggtagaat tggttgttga acagcttgct
    63841 gcttataaat gttgcactgt aaaatgcgga tagctgtctg tccacatgca tgaaaaggcc
    63901 ggcgtcggta agacagctgc gaagcatgca caaagcggca aaaacggctg ccgtctcctt
    63961 aaagctgtat ttttgcttgt tttttctcgt gaccaggaat aatccgctgc ttctgtccca
    64021 atatgaatca atccggtcat agagcttaag gaatgaagcc tcatatacaa tcaagtcatt
    64081 gacctcatgc agctttatca ggaaattcat acagttgcac aaggttatga aggagctgaa
    64141 gctatcatcg gaaatatttc ttgcaatatc gccggtatgg gccaaacgct cgcaaagctc
    64201 catgcctatt acattggctg tgctgtatac tgtttctttg tacacggcgg tatgccccag
    64261 aatggacaaa aggcacaagc ccacgactga cagatcccgt gagctgatat cgtttatatt
    64321 ttctgtaata ttctcacata ataccggcag cacttccaaa gccttcttaa gtctgggtac
    64381 taaatctctt gaatatgaat catcccgatc aatcagcccc aatatcgaag aaagggcttc
    64441 cgcaacaaaa aattgagttt tgatatccgg attttcgttg ttaatggtaa ttctatattc
    64501 accatagacg ttatcccccg tatcctctcc ggaataataa aagtctccaa gcttaagata
    64561 gtctatgagg aagcgggcct gtttaattga gtttacaccg aggcagtagg caataaattc
    64621 cttattctcc atttgtattt ccccattttg tataataggt atgagggcag tcatgctgca
    64681 tatggaatgg gccagtacat tgggttcaac ggatttcttc atctttttct ccagccagcg
    64741 gaagcttttg aagtcatagg aagcttccgg aatggctgca tatatgggaa tgcacctttt
    64801 ataaagggaa gacataaaag taatattttt aggcattctg atgctgcctg ccccttttag
    64861 aagcttgtat atatgactta actcctgttc atcaggcatc tctatacttc cgcagcccga
    64921 aagcaggcag aaggatatta cgttgagata tgtcattgtg ttgcattgtt ttattatgtc
    64981 aggatatgag aattcaaaga tcattgaaac accacccgat aaaaaatatt gcaattggta
    65041 tatctatata tgtttatgca gtaaaaggga tggataaaac taaaagctgg tactttaata
    65101 ataggcataa gatacataga cggataacat aaattcttgc gaatttatgc tataattaaa
    65161 ctatatattg actgctgaag aaggtgttga atgtgaagga agggaagatt cctccggaac
    65221 tattgaaaag cttggtattc aataatatca aggtacgaaa tgaagaagta atattgcggc
    65281 ccgaaatcgg agaggactgt gctgccatag acttcggaag ccatgcatgc gttttgtcca
    65341 ccgatccgat aacaggagcc gtcaagaatg ccggtgccct ggcaatacat atatcctgca
    65401 atgatgtagc agccagcggg gtaaggccgg ttggaataat gataacgatt ctggcgcctc
    65461 cggaagcggc agaaggggat ataaggaggg taatggagga ggcgggagaa gcagcctcaa
    65521 agctgggagt cgaaataatc ggagggcata ccgaagtaac ctctgcggta aaccgtattg
    65581 ttatatccac aactgcctta gggaaggtgc ggaaggacag aatagtaagg tcttcgggag
    65641 caaaggcagg ggatgatgtg attatgacca aatgggcagg cttggagggc acatcaataa
    65701 tagcctcgga taagggaaag gagcttgaag ccgttctgac accggaggag atagagtctg
    65761 ccaggtcttt tatcgataat atcagtgtag ttgaagaagg agtgactgcg ggggaattcg
    65821 gtgccaacag catgcatgat gttacggagg ggggaatatt aggggctgcc tgggagattg
    65881 cagaaagctc cggaaagggt atagacatat atattgacaa gatacctgtc tgggaggtta
    65941 caaaaaaaat ctgcagtttt tatggaattg acccctatag actgatatca agcggctcaa
    66001 tgcttattac cgcatcaaag ggagccgaac tggcaaagct gcttgagaag aagagaatac
    66061 cggctgcaat aatcggaagg ataaccgagg ccggcagaaa tataataaga aatgggaata
    66121 tttctctttt gaaccctcct gatacggatg aaatttataa agctttaaaa taaaccggaa
    66181 caaaacatgt aaattcccgt cgaaagatac gagcatatcg tatctttttt ctttgcggga
    66241 catgggggga cacggggacg tttctcctgt cccaccttca gtgggacagg agaaacgtcc
    66301 c
//
LOCUS       DAOQUR010000010        65106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_503_length_65106_cov_11.676715, whole genome shotgun sequence.
ACCESSION   DAOQUR010000010 DAOQUR010000000
VERSION     DAOQUR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 65106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65106
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_65106_cov_11.676715"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="ytvI"
                     /locus_tag="PKI50_03660"
     CDS             complement(<1..826)
                     /gene="ytvI"
                     /locus_tag="PKI50_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HNT02841.1"
                     /translation="MLQPDLEKYLNIILRILVYIAMALIALFAFKFVFAYFMPFVIAL
                     ILSMLIEPLVLFLQKLKLKRGISVVLSILLFLGGFVAISTFAITRIVYELMKLYNRLP
                     KYYDGIYNFSAEVIQQVTDLYLQLPQEALNIMQEVLRTVFGKITSFLSHTTTWLIDTL
                     TVLPSTLIFILITLIATFFITKDKGLIKDFIFRQLPPTWSAKITSLKTDLLVALIGFL
                     KAQSIILSVTFIESFIGLTIIGIDYAFILAIIIAILDILPVLGTGGIYVPWGITNLV"
     gene            1202..2671
                     /locus_tag="PKI50_03665"
     CDS             1202..2671
                     /locus_tag="PKI50_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT02842.1"
                     /translation="MNIDCIPILKSLRAYKNEGAVPFHMPGHKRGNIYKKTGLDFLSE
                     DILALDTTEVPGVDNLHCPEGAVLEAQGLAAEAFGADYTFFLVNGTTSGIYSMIMASA
                     NPGDKIIIPRNCHRSVYGAVILGRLIPVYIEPEIDYELGIAMGIKPETLESILEEHSD
                     AKAVVITNPTFYGICSDLKKIAEIVHNRGMLLLVDEAHGSHFCFNERLPISALEAGAD
                     MTSQSIHKTLPSMTQSSMLHVKSERVDIEKLKIFLQLVQTTSPSHILLASLDAARFIM
                     QQYGHELLDDAIDWSNWARSEINDTEGCCCLGPDRAGRYGAVDFDPTRLTVNFGASGL
                     SGTKAEEILRKNFKIQVEMSDLYNIVAITTIGDYRSDYETFVNAVKKLVETAEKRKAK
                     AMAPAVFFRPQEPSLMPWEAVYYEKEQVDMGQSIGRICGEMIIPYPPGIPVLMPGETI
                     SREIYEYLKLCVEYGIKLNGISDSKLKKVWVLKNNKKEV"
     gene            2675..3004
                     /locus_tag="PKI50_03670"
     CDS             2675..3004
                     /locus_tag="PKI50_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNT02843.1"
                     /translation="MKLIIAVVQDDDVEDLVETLVKEKVYSTKLASTGGFLREGNTTL
                     LIGVEKDKVDSVISIIKDICKSRKQTFTTPIPPTGSAGVYIPYPIEVMVGGATIFVVD
                     IDRFEKV"
     gene            3017..>3429
                     /locus_tag="PKI50_03675"
     CDS             3017..>3429
                     /locus_tag="PKI50_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HNT02844.1"
                     /translation="MKITEIKTHPSVISTVAKEDKKSDIGSSKSSFENTLRKTEEQDL
                     ASRLNRLMSDIEKQGDNLVKSMDIKELKKYKKLIAEFMDEVVNNSLKFSKQSHFDRRG
                     RHKVYALVRKVNAKIEELSREFLKDEKDNIKILES"
     assembly_gap    3430..3529
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3530..4554
                     /gene="holB"
                     /locus_tag="PKI50_03680"
     CDS             <3530..4554
                     /gene="holB"
                     /locus_tag="PKI50_03680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNT02845.1"
                     /translation="TEGEETMGFNDIIGQRVIVESLRNAVKSNMISNGYVFSGPKGCG
                     KKLMAFIFAMALNCGGEANDKPCGRCSSCMRTSSGNHPNLKIVRPIGQSIKIKQVRGI
                     ISDSARKPFESGYKIIIIDNADKMTIDAQDAFLKTLEEPPENTVFLILTENHSLLLPT
                     IVSRCQVYRFKPVDSLEMKAFIKSKYDYPAMDVEAAVRHSKGIVGSALELLRDKGNFK
                     ARALYIDILEKAMTGDGNDAILLASEAIEDKESAESFLEFSLSWFRDIMVLRESRGAY
                     DNLIINADSLEVLTRHNSFLTEAKLNSIMEIINKSYRYIKHNVGIKNSIDGMLFNIAE
                     VNSYNG"
     gene            4547..5428
                     /locus_tag="PKI50_03685"
     CDS             4547..5428
                     /locus_tag="PKI50_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HNT02846.1"
                     /translation="MVRTVGVRFKKAGKIYYFDPGDLEINIDDNVVVETIRGIEFGKA
                     VLGIREVPEEEIIAPLKKVLRIATEEDNRIYENNKLKEQEAFNICLQKINHHNLGMKL
                     IDVEYTFDNNKIIFYFTADGRVDFRELVKDLASVFKTRIELRQIGVRDEAKMISGIGP
                     CGKSLCCSTFLGDFAPVSIKMAKEQNLSLNPTKISGICGRLMCCLKYEHETYECARRY
                     MPKIGALVDTPEGRGEVVESNILLEMVKVKLELMEGNLRVIKDFHVKDIVQLRPGKDI
                     EPDIEGDIENLELLEDN"
     gene            5518..5688
                     /locus_tag="PKI50_03690"
     CDS             5518..5688
                     /locus_tag="PKI50_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT02847.1"
                     /translation="MAYKIQDTCISCGACESECPVSCISQGDDKYVINPDECIECGAC
                     ANVCPVDAPVQD"
     gene            5744..6484
                     /locus_tag="PKI50_03695"
     CDS             5744..6484
                     /locus_tag="PKI50_03695"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HNT02848.1"
                     /translation="MEQLVHRDETVDDLQNGFSIIQKKHSFRYGVDAVLLADFADIKH
                     RHSVIDLGTGTGIIPILIYSRKKPVEITAVEIQKDMADMAARSIVLNGLEKNIKVLCM
                     DIKDAPMLLGKSRYDCVVTNPPYMKKECGINNPSETKAIARFEIMCSLEEILIAAREL
                     LKPGGKLFMVHRADRLADIIYEMRDKGIEPKRIRFVHPSIGKRPNLLLIEGARGGNKE
                     LKFMDPLYIHDENGEYTEEIHRIYGRKV"
     gene            6484..7392
                     /gene="rsmI"
                     /locus_tag="PKI50_03700"
     CDS             6484..7392
                     /gene="rsmI"
                     /locus_tag="PKI50_03700"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNT02849.1"
                     /translation="MTGILYLCATPIGNLEDITLRCVRVLREADIIAAEDTRQAIKLL
                     NHFQIRKPLISYHEHNKHEKGKEIVELLRSGKNIALVSDAGMPAISDPGEELVRLCID
                     SGIEIIPLPGPSASLTALIVSGLSTRRFCFEGFLPSNKKERKERLKKLSAETRTIIVY
                     EAPHRLLDTLGEIMEYLGDRRISVSREITKKFEETVRGEISSVIGIFKERAVKGEFVL
                     IIEGISEKELVDIELKKWDNVSVEEHIAMYLQQGADKKEAIKMVAEDRKLPKKEVYKI
                     GCNLERRTKNAIHKGYDSSSDIKSKS"
     gene            7340..7534
                     /locus_tag="PKI50_03705"
     CDS             7340..7534
                     /locus_tag="PKI50_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNT02850.1"
                     /translation="MQFTKDMTVAQILRANPKTAEVFLRYGMHCLSCPGATGESVEQA
                     AMVHGFDADQILDDLNKVSE"
     gene            complement(7603..7842)
                     /locus_tag="PKI50_03710"
     CDS             complement(7603..7842)
                     /locus_tag="PKI50_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNT02851.1"
                     /translation="MKSTGIVRKVDELGRVVIPIELRRTLNIEEKDSLEIYVDGEHII
                     LKKYEPACIFCGNAKDVVQYKGKNICPACMEEFKK"
     gene            8089..9657
                     /locus_tag="PKI50_03715"
     CDS             8089..9657
                     /locus_tag="PKI50_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT02852.1"
                     /translation="MGKNTDTYHIDSDIPPESFWMASTARTNYPALGNDIKVDVAIVG
                     GGMAGITSGYMLKKEGLSVAVIEADRIIQGTTGHTTAKITSQHDLIYSKIKNYMGEEK
                     ARQYAVANETAIRVISDLIEQEKIDCDFHKESAYVVTLTDSYVQQIADEANTAGSLGI
                     KSSFIENTPLPFKVKGAVKFDGQARFHPRKYLLALAKKVDGGGSFIFEQTRVVDINKG
                     NPCILVTNLGKKVTADNVIIASHFPCFDGMGFYFARMYPDRSYAIAVRAKEKFLGGMY
                     ITAEDPGRSLRSQEYEGGELIIVSGEHHKTAHGTNLHRHYENLLEFAKKHYDVQEQLF
                     RWSTQDYTTLDKVPYVGRLTSKIQNIYVATGFRKWGMTNGTASAMILKDLILNGRNPW
                     AEVYDPSRFVLNPSITSFISINADVAAKLASGKLKPVPEDIEVEKGEAAIADIEGNKM
                     GIYRDKKGQLHRVDTTCTHLGCELKWNDAERSWDCPCHGSRFTYEGEIIEGPAISELQ
                     HGSNEKNKIDPNIV"
     gene            9935..10699
                     /locus_tag="PKI50_03720"
     CDS             9935..10699
                     /locus_tag="PKI50_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNT02853.1"
                     /translation="MGELTLGLVQMRCEKGAIEYNIISMYKYLEECRNKGVDIVCFPE
                     MNISGCINPLKYSNALLSMFHPSIQRIADMSFLYQTIIIAGFAEKNYGGKPYITQFVA
                     KDGKIEGCYRKRTLRGDQSKWFSPGTKPFMFRYSGYNFGLTISQDIYDGGIFKEYAER
                     GASLVFESAAPGLSVGQGVGDWEPGFSSWKNECKSKLGKYAAEDGIFIAAATQAGRTC
                     DEDFPGGGYVFAPDGRCIYEMEDGEEGMLVAKIQLN"
     gene            10766..11353
                     /locus_tag="PKI50_03725"
     CDS             10766..11353
                     /locus_tag="PKI50_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNT02854.1"
                     /translation="MINKLWFYMIAIAVAFAATGRRLEILTEEIFSSLGAAVELGIGL
                     LGGMMLWCGVLKVAEKSGLADKISDFIRPLMKLLFRGIPPKSAAMGAMIMNITSNMLG
                     LGNAATPMGLKAMQELQQLNPRKDTATNAMCLFLVVNAAPIQILPATVLSLRASVGSA
                     NPGIIILPAVASSFAAVSAGVITCKLLEKTCGGRA"
     gene            11350..11859
                     /locus_tag="PKI50_03730"
     CDS             11350..11859
                     /locus_tag="PKI50_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNT02855.1"
                     /translation="MNFIIPIIILLILIYGYLNGVKVYDAFIEGAGEGLLITFRILPY
                     IGAMLLAVGMLRNSGGLDFMLYVLSPLTSAAGIPEEVVPLIIMKPLSGSGALGILADI
                     LKRSGADNYAGVLASIIMSSTETIFYTITVYFGSIGIKNIRHTLAAAVIADMAGLAAA
                     VLLCRFILF"
     misc_binding    11925..12173
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12211..14115
                     /gene="metG"
                     /locus_tag="PKI50_03735"
     CDS             12211..14115
                     /gene="metG"
                     /locus_tag="PKI50_03735"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNT02856.1"
                     /translation="MTDKKTYYITTPIYYPSDKLHIGHTYTTVAADAAARFKRMLGYD
                     VMFLTGTDEHGQKIQRKAEEKGATPKQYVDEVVAGIKDLWKLMQISNDRFIRTTDRQH
                     EEAVQKIFKKLYDQGDIYKSEYEGWYCTPCESFWTERQLVDGKCPDCGREVELTKEES
                     YFFKLSKYQDRLIKYIEENPDFIQPQTRQNEMLNNFLKPGLDDLCVSRTSFNWGIPVS
                     FDEKHVVYVWIDALSNYITALGFLSDDDSDYKRYWPADVHLVGKEIVRFHTIIWPIML
                     MALGEPVPRQVFGHGWLILEGGKMSKSKGNVVDPVVLVEKYGVDAIRYFLLREVPFGS
                     DGVYSEEALVNRMNSDLANDLGNLLSRTITMIDKYFEGVIPAAKNSGEPLDADLIKLA
                     ESTPIKVEELMDKLLFSNALTEIWKLIGRSNKYIDETMPWVLARDESKKERLAEVLYN
                     LAEVLRIVGVLISPYMPNTPAKIFQQLGIEDEALKTWESIKSFGALKAGTIVNKGEII
                     FPRVEFGGENAAPETPKEDNYITIDDFAKIDLRVAKVLECEKVEGSDKLLKLQLEVGS
                     EKRQVVSGISKYYAPEDLIGKSVVLVANLKPVKIRGVESNGMILAADAGDSVVVVSPL
                     SEIPSGSKVR"
     gene            14232..15002
                     /locus_tag="PKI50_03740"
     CDS             14232..15002
                     /locus_tag="PKI50_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT02857.1"
                     /translation="MLFDTHAHLDDGRFDKDRDEAISQCKQDGVELILNAGSNIETSV
                     KAIALSKKYEFIYASVGVHPHDAASMDEETAGVLAALAGNRKVRAIGEIGLDYHYDLS
                     PRDVQKQRFIEQIDIARQLKLPIIVHDREAHGDVMDIFKKTRIKEVGGVLHSFSGSAE
                     MALECIKLGLYISISGPVTFENARKTVEAVKQVPLDMLLIETDSPYLTPVPHRGKRNY
                     PGYVRFVAEKIAEIKGISFEEVARQTLENGKRLFGIEE"
     gene            15427..16005
                     /locus_tag="PKI50_03745"
     CDS             15427..16005
                     /locus_tag="PKI50_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HNT02858.1"
                     /translation="MITYEINDALYINLTNRCTNNCSFCIRNHPEGFGFDLWLPKEPV
                     LEEIMADLENLELYNEVVFCGYGEPLVRYREIIGICRHIKQKGVRTRINTNGQANLIW
                     NRNIVPELKGLVDSISISLNAKNAEDYAELCRPEYGKAAYSAVLDFAGECIKYIPEVW
                     LTVVDVISAYDIEVCRGIAEGIGAGFRVRHYC"
     gene            16243..17280
                     /locus_tag="PKI50_03750"
     CDS             16243..17280
                     /locus_tag="PKI50_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNT02859.1"
                     /translation="MKINLKDFKSLFSIKLIAVAAICLIFAASYVYSAMKKEITIEDI
                     GGVKVVSTFKETVSDVLEENNIQLIPQDKINLGMTEKLEDGTHIKISRAKKVTISSDG
                     EDIVILTTAPDVKSALGEVQIKLGEKDRVSPGFDTAITGDTTIAVTRVEERIVKQEMK
                     MDFTNEVRKSDNLDKGLARVIKKGSQGSKELSIKVIFENGKEVKREIAEEKVLKEPVN
                     GIIEEGTRTTFVSSRGQVTRFVRALKMTATAYDATFESCGKHPDHPQYGITYSGLRVR
                     PGIVAVDPKVIPLGTYLYVEGYGEALAADIGSAIKGNKIDLYYESPEDVAKYGKRTVK
                     VYVLDKPKYKF"
     gene            17322..17864
                     /gene="rnmV"
                     /locus_tag="PKI50_03755"
     CDS             17322..17864
                     /gene="rnmV"
                     /locus_tag="PKI50_03755"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HNT02860.1"
                     /translation="MLKETIVVEGKNDAAAVRRAVEADIIITSGFGINGSILERIRTA
                     QDRNGVIVLTDPDFVGEKIRKAISDKVKGVKHAFLSKEEAEAKGDIGVENAAPESILR
                     ALSLAKYELEEVQATFTSEDLTYYGLMGGQNSAELRNRVGSALGIGYSNAKQFINRLN
                     KYGISREELEAALEKVSEQY"
     gene            17879..18736
                     /gene="rsmA"
                     /locus_tag="PKI50_03760"
     CDS             17879..18736
                     /gene="rsmA"
                     /locus_tag="PKI50_03760"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNT02861.1"
                     /translation="MKETSIKTSTLDLMKKYDFKTAKKLGQNFLTDETVLKTIIDCSE
                     LSKEDCVLEIGPGLGVMTQILCEKAGRVLAVEIDRKLIQILRVSLFGYENVEIINEDI
                     LKLDLKKVLEEKFGSSPVKIVANLPYYITTPIIMKLLEEDINLRSITIMVQKEVGERI
                     CAAPGGKDYGALTVAVQYRCIPRKILVVPPDSFMPQPEVESMVVRLDFRDKPPITLKN
                     EKMYFRVVKASFGQRRKTLLNALSAGNLGKTKDELKDILARNGIDEKRRGETLSLEEF
                     ARIANDLLS"
     gene            18816..20087
                     /locus_tag="PKI50_03765"
     CDS             18816..20087
                     /locus_tag="PKI50_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02862.1"
                     /translation="MASNNEYKRYFIILQEDDKGYELSPGKIPTGYVKIEVKNNKVRL
                     NAFIQNIKHEDRIEYRLILVSSAKKLAVDAGKIIIDGSGRGELSYELEPDNVLKSGLS
                     IGDFNVAAVTCGSSIPLSGYAGRDKLEWKNKYDIVNKPKVQEAPRIDKKIEIEEIVPE
                     VPQPPIHVPLAPQPEPAVVEPAPKPEPAPVPIMEAVPEPEPKPVPEPEVLIQPAPEPV
                     IIPESKVEVKIVIPECPPIAPMEEPCEIEKVKPIKDKCEYENLEEYYCYDDEEPCKHT
                     ASHYYSGGMYSRLKRVLRKLRRYEPFEKERGYSWFKVGDDMYEINRVSVPYMGYMMPL
                     GYPFLSEGCSMMMDRKDYILGVRYEDDDEDDDRGQIKYILFGVPAIYSKRNEMYYKSR
                     GFMEFRPHKTKSYGYFIMCLDIRRGMLCRMN"
     gene            20166..21281
                     /gene="rodA"
                     /locus_tag="PKI50_03770"
     CDS             20166..21281
                     /gene="rodA"
                     /locus_tag="PKI50_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNT02863.1"
                     /translation="MKVENLVDRKLLKNIDLGIVISTILLIAYGMAAISSATHIRSGG
                     SAGTLKVQIIAFILGIIGVIVILLIDYKTFGDNYVLIYIINVFLLALVLIIGFSTKGT
                     KGWIDLGPVNMQPSEIVKIGYILTFAKYLEKKKGNLNRIIDVLPAMLHLVLIVGLIML
                     QPDAGTALVFIFISIFMLFAAGISYKIIAGAFGAFLISLPVMWKLLKPYQQSRILVFL
                     NPELDPMDKGYHVIQSKTAIGSGQLWGKGLFEGTQNNLGFVLERHTDFIFSVIGEELG
                     LIGALVLMMLFLWLLLRCIHIAKVSKDDYGMLICVGIISMFLFHILENIGMTIGLMPV
                     TGIPLPFISYGGSSLLTNMAAVGLVLNVGMRRQVIRF"
     gene            21598..22842
                     /locus_tag="PKI50_03775"
     CDS             21598..22842
                     /locus_tag="PKI50_03775"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNT02864.1"
                     /translation="MDINKNKVVLAYSGGLDTSIIIPWLKENYDLDVIACCVDVGQRD
                     ELEPIKHKALSTGASKVYIIDAKKEFAEDYIIPTVKAGAVYEGKYLLGTAMARPLIAK
                     KLVDVAEKEGAGYIAHGCTGKGNDQVRFELTIKALNPDIKVIAPWREWTIKSREEAVD
                     YAAARGIPVPVSKERPYSMDRNLWHLSHEGVDLEDTKNPHPTDIYMLTTTPEDAPDKP
                     EYVEIEFNKGIPVKLNGIACDSLELIEELNVIGGRNGIGLVDLVENRLVGMKSRGVYE
                     TPGGTILCSAHKELEYITLDKQTMRFKQMVSDQYADLVYNGLWYTPLKEALDAFVDRT
                     QQNVTGVVKMKLYKGSCVISGMDSIYSLYNKDFATFGENVDNIYNQKDAEGFINLFGL
                     PIEVMALMKAKLAKQQEERKIG"
     gene            22843..24237
                     /gene="argH"
                     /locus_tag="PKI50_03780"
     CDS             22843..24237
                     /gene="argH"
                     /locus_tag="PKI50_03780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNT02865.1"
                     /translation="MKLWGGRFSKNTALEAEDFNSSISVDSRLYLQDIEGSKAHAIML
                     GKCGIISADEAAEILSGLEEIKQGIESGSIDLKKDAEDIHMNIEKLLIEKIGETGKKL
                     HTARSRNDQVALDIRMYLRDEIHSILNLLTELEALLLDKAEEHAGTIMAGYTHMQKAQ
                     PVTLGHHFMAYFEMFKRDMGRFKDCMKRMDIMPLGSCALAGTTYPIDRKMVAGILKFS
                     DITKNSIDAVSDRDFAIEFISCCAVGMMHLSRFCEELIIWNTGEFGYIEMDDGYSTGS
                     SIMPQKKNPDIAELIRGKTGRVYGDLIALLTIMKGLPLSYNKDMQEDKESVFDAGDTL
                     KSCISVFIPMLEAIIINKERIRNNLIGGFLNATDLADYLVKKGLPFRDAHEAAGEMVK
                     YCIAANKELTELSMDEFRKFSCCIEEEIYEFLKPEVCLSRRNTEGGPSEQRVREAVLR
                     GREYLNHFAETVKF"
     gene            24260..25219
                     /locus_tag="PKI50_03785"
     CDS             24260..25219
                     /locus_tag="PKI50_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02866.1"
                     /translation="MIWVGILGAKGYFGEELSKLIAGHQSAQVSTVLDLQELSESGCG
                     FGRSNGKSAGYLKMMNAVNRSDVIFNGFSGTAAEDIYSKALSNGKKIIDVGDKHIDGS
                     AYPGSVFGLSELYKDKMKDASIAANPSSYCTGAMLGLAPLAAGNLIDINTAVIESKSG
                     ISCLRCGDKLECAGITADDGSKIYKMESRDYAEEVKGQMLALFGKDALSSYVSYIIPG
                     IKGIITSITAEPGTGFCGSDISEIYRGFYKFNPLIEVSGADTINGARNGFDKYFCSIS
                     ASTDADTGKITVTTILDDAVRGSASQAVQTMNLMYGIDGKVGL"
     gene            25393..27048
                     /locus_tag="PKI50_03790"
     CDS             25393..27048
                     /locus_tag="PKI50_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNT02867.1"
                     /translation="MGFNVIIALFAGLSLFLYGMQIMSDALQKSAGDRLKKLLEILTT
                     NKYIGVLVGAAITAVIQSSSATTVMVVGLVNAGIMNLSQAVGVIMGANIGTTMTAQII
                     AFKLTNIVPYAIIIGAILVLFSRKKSRKHLGELIIGFGILFMGMNMMGDAMKPLKDIP
                     EFSQFMIGLEDNPLLGVLSGLLLTAIVQSSSATVGILQVLAMQGLVPIEAALPILFGD
                     NIGTCATALIASIGTGLTAKRAAIMHVTIKIIGTIVFMFILHPVTSLVVMIASEPARQ
                     IANAHTLFNITNTVILLPFSAMLITFVTKVLPGEDVYDKFALKYLDKRILETPSIAVG
                     QIVKEIIRMGEVAKCNVQKGMQAILNNDERVLDEILNNEKVINELERRIGEFLQEVMH
                     SAIGDDQQKMVGMLFNTVHDMERIGDHADNLGEIAQYKLDNKVSFSNYAMEELREIYE
                     NVEEAMDNAFLALSTKDTAYVEAVDAYERKVDELRDSFRESHINRINKGECNINAGVL
                     FLEILTNLERISDHCVNVADVVRIVDRSKLKLDRVSEISPEKC"
     gene            27146..27826
                     /locus_tag="PKI50_03795"
     CDS             27146..27826
                     /locus_tag="PKI50_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT02868.1"
                     /translation="MNNHDYLRQISIFSELEGEFLEKIYQISRVRKYKKGRIIFMEGE
                     PGEAFFYIKSGLVKISKLSRDGREHILHVLNEGHVFAEVTLFSNTEYPATAEVLEDAE
                     IGIIKNEDLEKVIKENPDLSLQLIKYLNKRLVEAHMKIRNLALYDTYERTAQALVKLA
                     EDYGKKSSKGVNLDINISRQELANIVGTTRETVIRALTAFKKEHLIGIEKNTITIIDL
                     ESLKEWPY"
     gene            28224..28427
                     /locus_tag="PKI50_03800"
     CDS             28224..28427
                     /locus_tag="PKI50_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02869.1"
                     /translation="MNNIIHQICENYISEVLHFFDSGAIRKLDEIEVVLKEKTNSYLK
                     QMTQTYFEEIDQAIADDKTGGRS"
     gene            28956..31955
                     /locus_tag="PKI50_03805"
     CDS             28956..31955
                     /locus_tag="PKI50_03805"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HNT02870.1"
                     /translation="MEGYKLRYKLITFLSSGNIKDISSVYAKTFDIALFIFPSRRSFI
                     PFDRIEAIISETLEVYSEKNLVQVPPFDNLEPTITNLGNVFFVLLKEALFQKDLVLET
                     LEISESPIRSYIVNEVNADRGFLVGHKKIKATSLLMDDIISQSTYHLLSENEKEGQTP
                     IPVVHAPAPVAEAGLPLPPPKPKIEDSSPTLQPVKVPVYQFTLCFLLLTVCGVLITIY
                     LKKTGAYPSGSDIYGHLFKSDFLYHSIRAGDFYPLYTDLWYNGMQPFRYWAPLPYYFL
                     VILQFIAGGDAISSYLLFVLFAFVIGGTGWLLWGWAYNRMLFTTFLGVLWFFLPDNIR
                     VFFVEGNFPRMAIAIFLPYLFYFVWRYVEHSKTWAVVPVIFIMCCIILSHVMIAAMTG
                     IGTFIFLFIYSMRQKRLLESFQIIVAMLLSFALCGVWLYPALQGGLMGMNAAATSEVM
                     QSLSTPISISLNPLLRTQEIFDYYYFGLSILVISIIGIFVADKKSLPGFLTSIIIFLG
                     TTTVVVPFLEKLPLNQLLWMMRFTPIVYAIFMLALLEWRNCRRYVAIIIAFFLIIDSI
                     PSAHLEKYFSHNPARLSDSLSTTKELTRQRVSLIDLSVFDSYPSYYFSSKEPKTKYTF
                     GWAWQGAATANNIMMINTAAEKGYYYYLFDRSLELGDDTVMVHKELVDKAKRTMTSLL
                     EAAQASGYKFYKETNFTYIFHRDTPACFGVASEYAGLAIGNSAKAIALEYPSFEEGSS
                     DNLTDYRYEDLSRYKVIYLSGFSYNDKKAAEELLTRTANGGVRIIIDMGRIPVDPVTS
                     RMTLFGVTAQAISFSAQYPELMYHDQVYQALPFKEEYSTWNTFYLDNVEQAIGYSWFK
                     NKNMPFLGTAGSPNIIFMGYNILFHAMETQDESIIMLINDLLEFEPTQLPDREVVSIS
                     VKKQKDKLIIDSEKGSINTTIAYQDNFRSDQKIYEKNNLLVVQEPHTEIEITYAYLAQ
                     GLALSGAGVLGTGVLLYFIIRKRRCVQ"
     gene            31952..32584
                     /locus_tag="PKI50_03810"
     CDS             31952..32584
                     /locus_tag="PKI50_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Firmicu-CTERM sorting domain-containing protein"
                     /protein_id="HNT02871.1"
                     /translation="MKKIIQKVLIGLLLIQAVSFSGYADYGSIDIDGYYDDWEDKPHT
                     EVYYGKKPYVSEIHLVSVFRDETTVYVHIKMSENGYTELPNSYFTMDTDMGSEAFTLM
                     LDSMKKKESGTAGIEVHLTDNWDVVGSGYYTREEGESDEAEFEIPLYSITDEPDGVVD
                     IAIMFPDLGSQDIVCVGAGTGPYIAVAIGAVIVLLSLAYYYKKKHPAYAK"
     gene            32597..33199
                     /gene="xrtG"
                     /locus_tag="PKI50_03815"
     CDS             32597..33199
                     /gene="xrtG"
                     /locus_tag="PKI50_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase family protein XrtG"
                     /protein_id="HNT02872.1"
                     /translation="MLTLKIIAFIVWIYVIWVLSRTGLHFFKFCIGSVGLFFFLMLWI
                     QPLIMQPLKMAVSAAAGVLGDFFEMYDSYYQYSILFIPKDTTAVSLTIDYECSGVIEI
                     MAFSCMLWFFPLYNTIEKLIINCIGLAVIFLANVLRIFLICTLIYFYGNEIFYFAHTI
                     FGRIFFYSISIALYFYVFTRSHIIRQKVGSFNYGNGDTDK"
     gene            33174..34547
                     /locus_tag="PKI50_03820"
     CDS             33174..34547
                     /locus_tag="PKI50_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase, exosortase G
                     system-associated"
                     /protein_id="HNT02873.1"
                     /translation="MEMEILINRIIREVIFWMAWIIIPLIMEIIPAFGGFFILLKKSF
                     VLKGKAIPQAVSEITLIIPIYNSAETLRTCLTSVHNSNYPIELMDILLVNNGSTDNSY
                     EVYRQCQDEFRDLPMTWLNASQGKSKALNMALYNSHGKYIVHIDSDGKLHPDALRNMA
                     IRFEQRPEVDCLTGVILTDMKLIESTNSFMMKLVRRCEFFEYCQSFLAGRNFESEFNS
                     IYTLSGAFSAFRKSAILKSQLFNTATVSEDTQVTFQMHKIQKKGISLCENAFFYVDPI
                     ESYNKLYTQRQRWQRGEIEVSHMFLQEELTIIKGFISNFMVRTLVFDHTFAFPRMIWY
                     FALIFLVFLNYPIALVLSSIGIIYLLYSFSMFLYYINVLMYLDKVKDIQRYYRSKWYL
                     IFVLPGFNFVVFWIRFAGIINSIKGEGVWKTTSLTEEWNKIKVAVNDDFHFPKRVIAA
                     LRRKVNL"
     gene            34544..35323
                     /locus_tag="PKI50_03825"
     CDS             34544..35323
                     /locus_tag="PKI50_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT02874.1"
                     /translation="MNTLTVALPAYNEEENIEALVSSWQQYREILMDKYGLILQIVVI
                     NDGSKDKTVEIAEDLKRKYSNFTLVNHIHNKGLGEAVKTGIAYFLDNCPESRYMCLMD
                     CDNTQDPCYITDMLDCSNRTGADVIIASRYRKGSEVMGVSGFRLIMSGGARYVFSVLL
                     NIPGVRDYTCGYRLYSREILRKASYRFEKDLIEESGFTCMAELLYKLFTCGAVFGEIP
                     FVLRYDYKKGTSKMSVLKTAINSMGLALRLRKIKMTEKSDE"
     gene            35316..37955
                     /locus_tag="PKI50_03830"
     CDS             35316..37955
                     /locus_tag="PKI50_03830"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HNT02875.1"
                     /translation="MSEITENKITDSKLQYRIELFSALIITGIVCAAGSSNLLFASND
                     LYPAATDAMGHMAKVQYITQCLKGGVIPSWFPYWYNGAAVTQYYPPLSYYLMVPVYAL
                     TGNVMLTFKINCFLMMFIGGMGVWYFCRKVIGKWCGCIAIVFYCLQPFLLSSLYIVGV
                     LAQGPVYAITPWLLYLVLSFIKEPNRQKALFCTIATALLILSHPMHAFIIVLCIMLVL
                     LMFIPSKRISLLNYTVLVVMMTFSLIITAFWSLVGVTGLENPTIPYILPGVAANVTAT
                     LKWFQPGNMGGLYFYFALPILVFTVIAAMALILRTTKKQVINAERFEIPFCITLTFFT
                     TMFSFGRQIPGFKLLPMTDSLVSGRILSLTALTGAIACAYLTFTVIGYIPTLRRMMRL
                     VILAIALSAFVLTIVHINPYKFSFPIGSYEKFEKRFESVAGDISVSPFDKGRYIWFSG
                     LSSDEANIPIAYGFTISEGWNMEGSPHNRALWNHLLCIATDTRDYLAKNFAFWNVRYF
                     MLPTQYDEMVIPLKEHLGFRLAYDTEFYTSQLPSSYFLTDNRNGLIFGIGAPGVAMEF
                     PYLVYEQRRDISDYSLAELSEYKLIYLCEPVVDTMRQKERIEETIKYLIDKGVRIIIE
                     PTPTKGFKLFDVAVSDVPAEDSLLLEKQYMSPLNSIIDNVKIDEKHGYFRALFGLDEA
                     YYKSVQNGGLLQNDIIGTKRVGKGEVLFLGMHLSQYLKAVYALNLGVPEDIKGYPSCS
                     DEIKALFGDIFKTYGVEQNFWPEPFPVNNVRWNYKGVDFDYSSQAAQEMTISITYTPR
                     WKATIDGKPVDVGQRENLITLQLPAGEHKVQLKYGLTKYAVIGYTTLIAGLLLFSLFL
                     RNYDINVINLRKLCALLKNYLQV"
     gene            38084..39778
                     /locus_tag="PKI50_03835"
     CDS             38084..39778
                     /locus_tag="PKI50_03835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT02876.1"
                     /translation="MFQELNKYKDSIIKNYSSTQESLVTEVSKKVKESLLTGSGEEGV
                     IDLLSNAGTSGSRYWFLYTASGAVFEKDYEETRKIKGKSASELIKFWKLQGGTGTEDF
                     EKLLLQKKNGSSVFTKSADYGEEIVSVKYFIANNTGYFLGMSTQHQYVLYVGRVNEHI
                     LYLYIFAGAVSLALLVLALFLCLTLYKGQKRCDKANKIIKDNNFHIEDLTKKLASKTD
                     TVKKASIFDDLTKHYNRKFFYNLLSKINDEHFQPISIVVLDINGMDRLNRTAYYRTGD
                     EVLEKTSGILQRLCLDTDIVARTGGAEFSVLMTVTNISEAYGITENIRRQFTGLNHAD
                     LTLSIGVAQMQPEDNSIFSALDRARKNLILEKLNDKNSSDYSIIAMLMEILGAYSLST
                     VAHCNRLREKALIIGKSLRLSNSELSRLAIAAQLHDVGKIGISDTILNKKEMLTRHEK
                     DCIYRHPDIGYEIIKLIPPLDEVAIDIRQHHEFYDGTGYPAGLKGEEISLCARIINVI
                     DSFDAMTNKSVYAKVKTPEEAIEELQKKSSIQFDPHIVDVFVKVFSDNIKVGSEIA"
     gene            40209..40721
                     /locus_tag="PKI50_03840"
     CDS             40209..40721
                     /locus_tag="PKI50_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNT02877.1"
                     /translation="MTYSQYRFKFYFNASHAIYLMGQLGESHPHTWELIIHGAKITDD
                     FAPFNEIEKKIEDFLSQYQDVFINTVQPFTTINPTLENICTHFKTSIQELLYLKGWLL
                     LSIELSETPTRAYVISMHDEAESKKSYYEKAGAETYQDVVDELVFEKLDSLFENREIA
                     AEKDTDGEQK"
     gene            41019..43499
                     /locus_tag="PKI50_03845"
     CDS             41019..43499
                     /locus_tag="PKI50_03845"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNT02878.1"
                     /translation="MKRIRGSRFLIKALVILLIASITFPGAVYSLESAKLIEDGQHIG
                     PGTYYRNMNYITPNGNFMVNMIESKFDDKYISVEVADSGSSLTNKPVSYQASLKNDEN
                     KRVIGAVNGDFFDMTTIKGLTYGTSIIEGEIKTAVKTSTILGVTDKGDCFIDTLNMEG
                     AAAYKYIQYPVDSVNRLRWADQTVIYTPFFGKTTVNTVAGTDIIVRGVELPLKAGKRY
                     TGKIEKIIPDSKITEIPSDGVVISMPVKAFRQLAGVPSGAEMSFYVDFDKKNLDFAVS
                     GAPRLLEGGQISAKVKERADALQRHPRTAIGIKDNKIYMVTVDGRQLGYSDGMTLYEL
                     VEFFLSQGVENAINLDGGGSTAMAVRKQGDAAAKLANNPSDGRERYVGNSIQLVSEAP
                     LSDPAILRFKDTSVKIYKNSSFKPSYYVLDKYYNLLPGDMGKVRFGIDVKTSKIAADG
                     TYSSGSQAGKGYIYAACGDTQSKMLVEIVDKVSSLAVTNDFVLLDPGEKTQLQVKAFD
                     ENGGNIIISPSAVKWTVEGELGTVDKDGVFTAGNNNGHGKITASAGTVTAAVGAKIGK
                     TPVIVADFGTLDNVEAKFIRSKAAIRHNQKGEPVRIGNISLRLDYDFQNTEGTSAAYV
                     TFKKPVTIIGKPIELGVWVYGDGSSHWIRGNYINSAGEKKVFDLTKSGGLDWKGWKYV
                     YAELPKDEKYPIALEQIYLAETAQDNKNSGSIYFDDVMAIYKLDKDYYDPVIVSVLSE
                     SSEGLPGQAQEIGVFAADRGYGIDPASIKMYINDSAVKASFEPETGKISYIPAKPLKK
                     GEYKVRISLKDKIGHSLNPEYAYTFTVK"
     gene            complement(43585..44241)
                     /gene="trmB"
                     /locus_tag="PKI50_03850"
     CDS             complement(43585..44241)
                     /gene="trmB"
                     /locus_tag="PKI50_03850"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNT02879.1"
                     /translation="MRIRNNPKALEIIKQYDEIVIFRPGEYKGNWNEYFSRRTGNKED
                     APIRAELGMGRGRFISTNGLLNRDINFIGIDLRHEIILSALSKVLEMELQNVILIPYN
                     ILDIEDAFEPSEVDTFYINFCDPWPKVRHAKRRLTHRLFLGKYRTLLKNGGRIEFKTD
                     SRELFDFSINEFKASGFEILDLTYDLHNEDYPGNITTEYEEKFIKKGIKINRLEAVKK
                     "
     gene            44448..45677
                     /locus_tag="PKI50_03855"
     CDS             44448..45677
                     /locus_tag="PKI50_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNT02880.1"
                     /translation="MQLSTKILIGLAAGILAGILLQGSPDVANAWIKPFGTLFLNLIK
                     MIIVPLVLASLVVGTCGLGDVKKLGRIGGKTIAYYLLTTAFAVILGLILANIFSVGAG
                     YVLPADVKAAEPSKIPSFVDTLLNIIPTNPIKAAADGNMLQIIAFALILGSGIVVLGD
                     KGKPVLDFFDSLAEISYKITGGIMILAPYAVFALITPVVASNGPKVLLPLLSVIIIVY
                     VGCALHAALVYSGTLMLFAKFSPMKFFKGIFPAQAIAFTTCSSSATLPVNMQCTEENL
                     GVPKGISSFVLPLGATINMDGTALYQGICALFIANVYGLELTMAQQFTIVLTATLASI
                     GTAGVPGAGLIMLTMVLQTVGLPLEGLALVAGIDRILDMARTTVNITGDSACAVIIAA
                     SENELDRDCQIKHTYSA"
     gene            complement(45713..46657)
                     /locus_tag="PKI50_03860"
     CDS             complement(45713..46657)
                     /locus_tag="PKI50_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNT02881.1"
                     /translation="MKKYKALFSEAAILLLLVSSLGGCTYDPARRYIQTDPNTNNMAA
                     PNTPAIPNNVVGRADNNADGNKISATYEVVDFSYITCNTNNLNVRAGAGNKFPSVGTL
                     RSGQKIRALGKLDGWYVVKMPDSGRIGCIPSASARPYSTSANTGTTGAGTVTPSPNQG
                     AITGAGAGAGTTEAGTTGGGTAAGSGAMSSDESRILQLVNAERAKAGAKALSASSDCT
                     RLARMKSQDMADNNYFSHQSPTYGSPFDMLKSNNVSYMYAGENIAMNQSAEAAFKAWM
                     NSEGHRKNILNPNFTELGVGIAPKGNGSYIYTQLFIGR"
     gene            46858..47376
                     /locus_tag="PKI50_03865"
     CDS             46858..47376
                     /locus_tag="PKI50_03865"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNT02882.1"
                     /translation="MSILIQALLIFFARIIDVSIGTMRTILLVKGQRRIASVLGFFEV
                     MIYLIVLGKVVGNIDKPMLILAYCSGYAAGNIIGSKIEERLSIGRLVVQVIVKEALEE
                     LVESLREAGFGVTIIEGEGKEGKSYMLNIILDRKQVKTLYEIVDGCDCGAFITTMDVR
                     SSLGGYFQPKKM"
     gene            complement(47363..48238)
                     /locus_tag="PKI50_03870"
     CDS             complement(47363..48238)
                     /locus_tag="PKI50_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT02883.1"
                     /translation="MKKSLKADLALILVTIFWGAGFPATKIALQTMTPFYHIGIRFAV
                     ASLLLSLLFYRKLRSLNKSLIKPALILSSLLFATYAFQTVGIQYTTASKSGFFSGLAV
                     LIVPIFSIFYLKTKLELKTIISVATATLGLFLLSYTGSDFNFNIGDFLTILCSICYAW
                     QLLFTGTYVQKHDATLLAIVQLFFVSLYGMAFAVILEPIPANMSVPSFWSLMFSAVFC
                     TAFAFWMQTTAQKFTAASHIALIFTMEPVFGALTSFLLLNEVLGARGIIGGILIVSAM
                     IISEFQWAKSQSTSS"
     misc_binding    48384..48662
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            48708..49946
                     /gene="tyrS"
                     /locus_tag="PKI50_03875"
     CDS             48708..49946
                     /gene="tyrS"
                     /locus_tag="PKI50_03875"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNT02884.1"
                     /translation="MVPKYNVYDVLKERGFIAQVNHEEELRELLGKECITFYIGFDPT
                     ADSLHVGHFLQIMVMAHMQKAGHRPIALLGAGTAMVGDPSGKSDMRKMLTFEMIDHNA
                     RNFRKQFEKFLDFRNDRAIMDNNANWLLKLNYVDFLRDIGVHFSVNRMLSAECYKSRY
                     DKGLTFLEFNYMLMQSYDFLELYRKYGCRLQLGGDDQWSNIISGTDIIRRVEGADAYG
                     MTFTLLTTSEGKKMGKTESGAIWLDESKTSPYEFYQYWRNVDDADVEKCLALLTFLPM
                     DEVRRLGSLPGERINEAKKVLAYELTRMVHGEKEAAKAQNTAESLFGGSGDSEHIPTT
                     ELSKEQLDKGVNIVDLLVMTGLAPSKGEGRRLINQGGVLVEDTRVESIDLIIGTANLK
                     DGKLIIKKGKKTYHRIKIKE"
     gene            complement(50078..51652)
                     /locus_tag="PKI50_03880"
     CDS             complement(50078..51652)
                     /locus_tag="PKI50_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNT02885.1"
                     /translation="MEIFHNEYFSLFSVNGELYICVYHTGYEIREFNSLLMDMPFLQL
                     NSFTTLKNALDEASRLRICIGKIKPRVEVEISDDEMEASIMLNISAKEFAENKVPISS
                     EIIEALNNAGVVFGHDNIFKKPITVQKKIKAAKGTKPEDGKDTVIKYYEIPDKKPIVK
                     EDGTVNHYELNLIDNVKKGDWLGEKIPATEGKPGITVTGKVLPARKGIDLTLKYDKKT
                     VEEHEEGGKIVLRAKTDGAVKFENDKIKVDNHLIITKDVDYETGNISFEGYVTVKGIV
                     KDGFSVTAKNDISIQGNMGLGVINKIISKEGSIYIKGGIFGKNISEIQAKKSVFIKYC
                     NECKIVAGEDINIGFYSLDSHLEAKKVILDPMHGKIIGGSVNAEIQVVTGIIGNRSEK
                     KTYISVSGFNREAIRKEIEQLSQKYSVTLNEAAKAKKQIESFEINIPGAEYVNSREYH
                     QCIRKYENILDEIKLLDEYRKKLERILETKGEGEIDVSKAAYPETYIQIKNMQKKIDS
                     IVKGSFYALDKKLHHN"
     gene            51933..52259
                     /locus_tag="PKI50_03885"
     CDS             51933..52259
                     /locus_tag="PKI50_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02886.1"
                     /translation="MDKNIDVYIRRLIELDSRAVELKRERDAEISELEMRGENELKGL
                     DEELEEAVIKAKQRRDEIIEAGRLQAKEMEEAVNLKIDKLQTHFENFKEDAARDIWKQ
                     LLAIER"
     gene            52265..53308
                     /locus_tag="PKI50_03890"
     CDS             52265..53308
                     /locus_tag="PKI50_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNT02887.1"
                     /translation="MGNITRFAAVNTKIKSMEGELLKDEDYTLLLGLESVTEVAGYLK
                     ERTAYSEVLAEVDTGTIHRGMLENLIKQKMVKSIDRIIHYFTGDYKSFVKTLYAKYEI
                     EELKILARAVYNGKDAAAFRNNVFVGKHSRIDTSKIYEAKYIRDIIYALQGSEFYKFL
                     IPLVDGDFSENPFRFEMVMDMSYYSILQNKWSKLEKRDIKALEQAQGIIADLLNIQWI
                     YRGIKFYRLSPEELLNYTINIGYRMDFNMLKTLCYSKSLEEFNRIVKSTRYGFMLKHD
                     GTTDIYMERRMERYICFELKSMMKNNALSIISAFAFIIFLQFEVKDIISIIEAIRYKV
                     PADQAYKYIVRKI"
     gene            53325..55313
                     /locus_tag="PKI50_03895"
     CDS             53325..55313
                     /locus_tag="PKI50_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNT02888.1"
                     /translation="MAVERMKITSIIGKLTDLDKVSRLIVINGSTHILNAISELNSNN
                     LNLSVPQKHMQILQELVNIKPYISGRDFSGDEGIIRLFEDLFNLKLKICEDYLCVGDE
                     YENTMAELRKVHEDVRHISEDMAERAANIEKLQQYLNNVSYLRNTDLRLEEINNLNFM
                     KFRLLMLSNENFKKLKLNIENVPAIIFQAAVFNGNTVIASITPKSLEEEAERIFISLN
                     YKELELPQEFSGTIEDVINQINAKSSNEKRQVEELRESVAEMSRTYSDTVFKAYTLLE
                     LEKKAEKLKQETALGSSMFFVFGFVPESEVEGVRIELKQYFGESIIFIADDVEDKRLG
                     NAPPTKMKNISLFKPFESLISMYGMPSYGEKDPTPFFALSYMLMFGAMFGDMGQGLII
                     LLAGLLISYRTANKSFGGILTRLGVSSMIFGALYGSIFGSEELIGALLIRPMANINTM
                     LIGAIVLGIILITISYMYSLLNHYSSGNIEEGLFGREGLTGFLFFLTLVFGALGKATG
                     ELTVGIGLPIGIMVVLLLIMVFKQPLAGMLTGRRLYEDSPGDYYIEAGFGVIETILSV
                     ASNIISFIRVGAFALNHVGLYIAFAAMAEMLNSKTAGIMVLIIGNIFIIGLEGLIVFI
                     QSLRLEYYELFSKYYSGYGVEYKPVGLFGKGDSGNEMV"
     gene            55366..55785
                     /locus_tag="PKI50_03900"
     CDS             55366..55785
                     /locus_tag="PKI50_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HNT02889.1"
                     /translation="MNTILGIAIGIVIFTIGLGVVLYRSAANIQGRKIRNILRASLVS
                     YMFLIALFLFILTPDIVNAAQEGDSGAGMGFIAAGISTGLATIGAGYAVGAVGSSALG
                     AVSENPRILGKTLIFVGLAEGIAIYGLIVSIMILGAL"
     gene            55825..56133
                     /locus_tag="PKI50_03905"
     CDS             55825..56133
                     /locus_tag="PKI50_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNT02890.1"
                     /translation="MRFFLLSDNIDTLTGMRLAGINGVLVHEREEILAAFDEACSSND
                     IGIILMTELLAEKVPEELKAIRLDASKPIITIIPDRHGERRRRDYITSYIKESIGLKI
                     "
     gene            56145..56771
                     /locus_tag="PKI50_03910"
     CDS             56145..56771
                     /locus_tag="PKI50_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNT02891.1"
                     /translation="MSVSVEDKIELFRNIIFKDIEETAYDIRHKAAEGFNQNKDRLLQ
                     EVEIRKNQIMQEAEKKAEKEKQQLIAKTKSQGQHRLLEKRQQSINEITEMLIRKADSF
                     VSEEGYKEYLAASLKKAAVDFENAASVQFAFTKRDLRTLKGFIEQEIASGELKDRCQL
                     RESEKNIIGSFYAEDEKREIQADYTLRSLIEENRELIGSSIYRRLDEV"
     gene            56776..58560
                     /locus_tag="PKI50_03915"
     CDS             56776..58560
                     /locus_tag="PKI50_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNT02892.1"
                     /translation="MDTNKGKIVYINGPVVKVAEANDLKMREMVLVGRRQLIGEVISV
                     DKETATIQVYEETTGLMRGEGIISTGRPLSVKLGPGIIKYIFDGIERPLLSMYEAGTA
                     FIPEGIGMITIDEEKLWDAEIIVGIGDYLDSGDIYAKVQETSLIEHRLMVPPGISGRV
                     VFAEKSGSYNIEHVLVKLETETGLHELKMYQMWPVRGARPVSRRNPIAKPLITGQRII
                     DSFFPLAKGGTAAIPGGFGTGKTITQHQLAKWSDADIIVYIGVGERGNEMTEVLEDFP
                     KIIDPKSGKSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYNIAIMADSTS
                     RWAEALREISGRLEEMPAEEGYPAYLPSRLAQFYERAGDVEALGGRDGSITIIGAVSP
                     AGGDFSEPVTQNTKRVVSTFLALDKSLAYARHYPAINWLKSYSGYITDLSLWYEENIA
                     PDMMELRAKLLRLLQEEEKLMEIVKLVGEDILPDDQRLILEISRVIKVGYLQQNAYHK
                     NDAHVSLKKQYGMLRVIDRLYDKAHNCVKMGAPISKVKNDELFYKITTMKYNVPEDKP
                     EMFDELINEIDNFYDSLKTMYGREVSRQ"
     gene            58557..59930
                     /locus_tag="PKI50_03920"
     CDS             58557..59930
                     /locus_tag="PKI50_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNT02893.1"
                     /translation="MRLKYLKLQKVEGPLIILSGAKEAVFGEVVDIEVEQEGHKKGKV
                     VQIDGDKVIIQVFESTSGISLNNASVSFTGKPMEIPLSRDILGRTFNGIGEPIDGAGF
                     YADSKYDVNGRPINPVGRRYPRNYIETGISSIDGLATLVRGQKLPLFSGDGLPHNELA
                     AQIVKQAKISGDDESNFAVVFAAMGIKHDEADFFRKQFDSSGVASRVVMYINLADNPI
                     VERITAPRCALTAAEHLAFVHNMHILVIMTNMTSYSEALRELSASREEVPSRKGYPGY
                     LYSDLASLYERAGMIKGSKGSITQIPILTMPNDDITHPVPDLTGFITEGQIILSRELH
                     QKAIFPPINVLPSLSRLQKESIGEGFTREDHPDVANQVFSAYSHVQDVRSLAQVIGED
                     DISEMDKRYMEFGRQFEQKFLNQGSEVNRSMEDTLDIMWELLTFLPKSELDRINPELL
                     DKYYGKR"
     gene            59939..60562
                     /locus_tag="PKI50_03925"
     CDS             59939..60562
                     /locus_tag="PKI50_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNT02894.1"
                     /translation="MEIIYTKTSLIAAQNSLELSRKGYELLDKKRNVLIMTMMSFIEK
                     AEEIQKKVEVVFGDAYEALQTANTTMGISNIEQIAQSIPEASGFEVLDKSIMGVEIPE
                     ISHQEEKLIHYYSFYHTNTALDVAMQKFHEVKLLLYELSEIEDSVYKLAMEVKRTQKR
                     TNALQNIQIPKYEEIVGKILEVLEEREREDFFRLKVVKKKGEKKAAK"
     gene            complement(60576..61865)
                     /gene="hcp"
                     /locus_tag="PKI50_03930"
                     /gene_synonym="priS"
     CDS             complement(60576..61865)
                     /gene="hcp"
                     /locus_tag="PKI50_03930"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNT02895.1"
                     /translation="MFCNQCEQTPHGGCTKVGVCGKNEDIAALQDTMIFGLKGIAAYA
                     THAREMGYIDPEVDGITHEALYMSLTNSNFNLKEHIDMVLKVGSAAVKVMELLDKAHT
                     ARLGVPVPVTVSQDKIQGRCILVTGHNLYALEELLKQTEGKGINIYTHSEMLPAHGYP
                     ELKKYSHLKGNAGKAWYDQRKLFEEFPGAILGTTNCLMPIRGTYSDKFFSYGTAGLEG
                     VQKIINDDFSPIIEKALSLPEVDIESDKTLITGFHHQTVLSIAPEIIDAVKTGKIRRF
                     FVIAGCDAPTKGRDYYRELALSLPSDCVLLTTSCGKFRFNDHDFGTVPGTNIPRYIDL
                     GQCNNSGSAAKIAIALAEAFNCSVNDLPLSIVLSWFEQKAVAVLLGLLHLGVKNIHIG
                     PKAPEWFTSNVAEVLQKNFNLQLISGDAKADLEKMLG"
     gene            62105..62635
                     /locus_tag="PKI50_03935"
     CDS             62105..62635
                     /locus_tag="PKI50_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02896.1"
                     /translation="MTEYKGRWEAIRGSLIVTAALIALITCIYVLVPHGFWAYFFIGL
                     LLLFIPTAYSGLILKITMDEKKIVVVRPFTRVTVKFEDVALCAVNCVGDGKYLIYAFV
                     KQRYRGGYTVKGIKPKLPFDEVIKMSLKEECLDLDVNFNRAKKIPVSFVENGEELKDR
                     FLLEVGKHQVKIMKEV"
     gene            62784..64268
                     /locus_tag="PKI50_03940"
     CDS             62784..64268
                     /locus_tag="PKI50_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNT02897.1"
                     /translation="MKEKKFFAVIIALLFVLMLAVGCSYNQAAPAITTNPIPADAEQP
                     QETTVTEVYEAPDPIESDQDEKLGVVYISASKLKVRESAGTDAKALDDLIKGTAVDIV
                     EEKMDDSGKAWYRISYDGITGSNEGWIAAEYTVRDRTELLDENLRKLDFSPQNKTYEY
                     PGNPRVQARGIYLTKYSASNDRLDKLIEMTKRTKINTFVIDVKDDKGHMLFPTKAAEK
                     YSPEANKYATVKDIGALMKKLKENNIYTVARIVSFKDPTYTKQHPDRAIIYRDSGKPF
                     TNSDGLIWASAYDKVLWEYNLEVSKEAAETGFNEIQFDYVRFPASDGGKLDKSLDYRN
                     NEGESKPQAIQDYLKYAREQLAPLNVYISADVYGLVGSVADDMSLGQYWEAVSNAVDY
                     ICPMMYPSHYANGTYGLPVPDAYPYETVFNSAKDSAARNKNIETPGAIRPWIQDFSAP
                     WVPGHIKYSDKQVKEQIRALEENGIGEYLLWNAGNRYSEGAVTE"
     gene            complement(64357..64812)
                     /locus_tag="PKI50_03945"
     CDS             complement(64357..64812)
                     /locus_tag="PKI50_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02898.1"
                     /translation="MALACFYGAIAAAWIIFLICALKKSISFKHIMVAIAGMGFSLLY
                     ETALGEYAGLYHYINQSDSLLYIIISGVFLYPVIEMLYALFLPERIKPAIVYTVICIM
                     LLLVFEILSLYTRTIVFTGWRIVPWSIATYILTFVWVNLLFRYLKKKGL"
BASE COUNT        20229 a        11351 c        15394 g        18032 t
ORIGIN      
        1 atacaagatt ggttatcccc caaggaacat aaatgcctcc ggtgccaaga acgggcaaaa
       61 tatctaaaat agcaataata attgccagga taaatgcgta gtcaatacct ataatagtca
      121 accctataaa ggattcaata aatgttacac ttaatatgat ggattgagct ttcaaaaagc
      181 ctatcaaggc tactaaaaga tctgttttaa gtgaagttat tttagcactc catgttggcg
      241 gaagctgcct gaaaataaag tccttaatca agcctttgtc ctttgtaata aagaatgttg
      301 caataagagt aatcaatatg aatatcaagg ttgacggaag tacggttaag gtatctatta
      361 gccatgtagt tgtatggctt aagaaagacg ttatttttcc aaagacagtc ctcagaactt
      421 cctgcatgat attgagggct tcctgaggca gctgcagata taagtcggtt acctgctgaa
      481 ttacttcagc tgaaaagtta tatatcccgt cataatattt tggcagacgg ttatatagtt
      541 tcattagctc atacactatc cttgtaattg cgaaggttga aatagcgaca aatcctccaa
      601 ggaaaagcaa aatagacaat actactgaga tgccccgttt aagcttaagc ttttgaagga
      661 ataataccag cggttcaatt aacatggaaa gtatcaaggc aataacaaag ggcataaaat
      721 atgcaaaaac aaacttgaat gcaaataatg caatcaaggc catggcaatg taaaccaaaa
      781 ttcttaatat tatattaaga tatttttcta gatccggctg aagcataaca tcacttccaa
      841 aaatatttaa atctgctgaa agaatcccat actactaaat atgctgataa aaagaatatg
      901 caactatata tttatattat attaaatgaa agtgtaataa tcaaacaata tcgaatatgc
      961 agagctgttt tttggagcat aatgccaatt tggcaattat tttggagtgt cttgtagaaa
     1021 ctttcatatc atcatattat aaacaaccaa gagcatatat agtataatta aagaaataaa
     1081 attatttcag cttaataatt gccaagaacc agataattat acaagagaca aaaaagtttc
     1141 tcagttagaa taggaaatac cggttaaata attaaaatta ctgatacgga agggatatct
     1201 gatgaatatt gattgcatac ccatattgaa atcgcttaga gcatataaga atgaaggggc
     1261 tgtgcctttt catatgccgg gacataaaag gggaaatata tacaagaaaa caggccttga
     1321 ttttctgtct gaggatatat tagccttgga tacgactgaa gtgccaggag ttgacaatct
     1381 gcactgcccg gagggtgcgg ttctcgaggc gcaaggcctc gccgcggaag catttggagc
     1441 ggattataca tttttccttg ttaacggcac cacctcgggt atatattcaa tgataatggc
     1501 atccgcaaat cccggagaca aaataattat tccccgtaac tgccatagat cggtatatgg
     1561 tgcagtaata cttgggaggc tgataccggt atacattgag ccggaaatag actatgagct
     1621 tggaatagca atggggataa agcctgaaac attggaaagt atactagaag agcacagtga
     1681 tgcaaaagcg gttgtaatca ctaatcccac tttttatggg atatgttctg atttgaagaa
     1741 gattgcagaa atcgtacata ataggggaat gctgctgctt gtggatgagg ctcacggttc
     1801 acatttctgc ttcaacgaaa ggcttcccat atctgcactt gaggcgggag cggacatgac
     1861 atctcaaagc atacataaga ccctgccttc tatgactcaa agttcaatgc tccatgtgaa
     1921 atctgaaagg gtagatattg agaagctgaa gatattcttg caattggttc agacgacgag
     1981 cccttcccat atactgctgg catctttgga tgcggcaagg tttattatgc agcaatacgg
     2041 acatgagctt ttggatgatg caatagattg gagcaattgg gcgagaagcg aaatcaatga
     2101 tactgaagga tgctgctgtt taggcccgga cagggcaggc cggtatggag cggttgattt
     2161 tgatccgaca aggctgacgg taaattttgg agcgtcaggc ctaagcggta ccaaagctga
     2221 ggaaatattg agaaagaatt tcaagataca ggtggaaatg tccgatttat ataatatagt
     2281 tgcaatcaca acgataggag attacaggtc tgattatgaa acttttgtaa atgcagtgaa
     2341 aaaacttgtg gagactgcgg agaaaagaaa ggcaaaagct atggcgccgg cagtattttt
     2401 caggccgcag gagccttccc ttatgccgtg ggaagcggta tactatgaaa aagagcaggt
     2461 ggatatgggg caaagcatag gaagaatctg cggagaaatg ataataccgt atcccccggg
     2521 aatacctgtt ttgatgccgg gagaaacaat cagcagagag atttatgagt atctgaagct
     2581 ttgtgttgaa tatggaataa agctgaacgg gatttctgat tccaagctga aaaaggtatg
     2641 ggtgttaaaa aataataaaa aggaggtatg agggatgaaa cttataatag cggttgtaca
     2701 ggatgatgat gtggaggatc tggtagaaac gctggtcaag gaaaaggtct atagtacaaa
     2761 gcttgcttct acgggcggat ttctcaggga agggaataca acacttttga taggtgttga
     2821 gaaggacaaa gtggattcgg taatatcaat aattaaggat atatgcaaaa gcagaaagca
     2881 gacctttact acgccaattc cgcctacagg ctctgcaggg gtttatattc catatcccat
     2941 tgaagtgatg gtcggcggtg caactatttt tgtagttgac atcgacaggt ttgagaaggt
     3001 ctaagggtga agtattatga agataacaga aatcaagacc catccttctg taatcagcac
     3061 agttgcgaaa gaagacaaaa aatcagatat cggcagttcc aaatcaagct ttgagaatac
     3121 tctcagaaag acagaagagc aggatttggc cagccgcctg aacaggctta tgagcgatat
     3181 tgagaagcaa ggggataacc ttgtcaaaag catggatata aaagaattaa agaaatataa
     3241 gaagcttatt gcagagttta tggatgaggt agtgaataat tcccttaagt tttcaaaaca
     3301 aagccatttt gacagacggg gaaggcataa ggtatatgcc cttgtgagaa aggtcaatgc
     3361 caagattgag gagttaagca gggaatttct caaggatgag aaggataaca taaagatact
     3421 tgaaagcatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taccgaggga
     3541 gaagaaacga tggggtttaa tgacataatt gggcagaggg taatagttga aagcctgaga
     3601 aatgcagtga aaagcaatat gatttcaaac ggctatgttt ttagcggacc taagggctgt
     3661 ggcaagaagc taatggcctt tatttttgca atggctttga actgcggcgg tgaagccaat
     3721 gacaagcctt gcgggaggtg cagctcttgt atgagaacaa gcagcggcaa tcaccccaat
     3781 ttgaagattg ttaggcctat cgggcagagc attaaaataa aacaagtccg cggcataata
     3841 agtgattcag caagaaagcc ctttgaaagc ggatataaga taataataat agataatgcg
     3901 gataagatga ctattgatgc acaggatgca tttttaaaaa ctcttgagga acctcctgaa
     3961 aacactgtat ttttgatact tacggaaaat catagcctct tattgcctac aattgtctcc
     4021 agatgccagg tatatcggtt caaacctgtg gacagcttgg aaatgaaggc ttttataaaa
     4081 tccaaatatg attatccggc aatggatgtt gaagcagctg taaggcattc aaagggaata
     4141 gtcggaagcg cgctggagct tctgagggat aaaggaaact tcaaggctcg tgcattatat
     4201 attgatattc tggagaaggc aatgacaggg gatgggaatg atgcaattct tctggcatca
     4261 gaagcaattg aggataagga atctgcagag agctttcttg agttttccct gtcctggttc
     4321 agggatataa tggtattgag agaaagccgg ggggcttacg ataatcttat tatcaacgct
     4381 gacagcttgg aggttttgac gaggcacaat tcttttttga cggaagccaa gttaaacagt
     4441 ataatggaga taattaataa gagttataga tatatcaagc acaatgttgg tataaaaaac
     4501 agcattgatg gtatgctatt taatattgcg gaggtaaatt catataatgg ttagaacagt
     4561 tggagtaagg tttaagaaag cagggaagat atattatttt gatcccggtg atttggaaat
     4621 aaacattgac gataatgttg tggtggagac cataaggggt atagagttcg ggaaagccgt
     4681 attgggaata agagaggtac ctgaggaaga aataattgcg cctcttaaaa aggtgctgag
     4741 aattgcaaca gaggaagaca acaggatcta cgaaaataat aagctgaaag aacaggaagc
     4801 ctttaacatt tgcctgcaga agattaacca tcataatctt ggtatgaagc ttattgatgt
     4861 agaatatacc tttgacaaca acaaaatcat attctatttc actgcagacg gacgtgtgga
     4921 tttcagagag cttgtaaagg acttggcttc agtttttaag acaaggatag agcttcgtca
     4981 gataggtgtc agagatgaag ccaagatgat aagcgggata ggcccttgcg gcaaaagtct
     5041 atgctgttca acgttccttg gagattttgc gcctgtgtcc ataaaaatgg caaaggagca
     5101 aaacctgtcc cttaatccca caaagatatc gggtatttgc ggaaggctca tgtgctgtct
     5161 taaatatgag catgagacat atgagtgcgc cagaagatat atgccaaaaa taggggcatt
     5221 ggtggatact cccgaaggcc ggggtgaagt ggtggaatcc aatatacttc ttgagatggt
     5281 caaggtaaag cttgaattaa tggaagggaa ccttcgtgtt atcaaggatt ttcatgtaaa
     5341 agatatagtt cagttaaggc cggggaagga tatcgaacct gatatagagg gagacataga
     5401 aaacctggaa ctgcttgaag acaattagtg aaaaattaaa caaaaacagt acaaggggtt
     5461 ttatttataa tagaatagtg atagaatgta tttgtaattt atttaggagg tgctactatg
     5521 gcatataaaa ttcaggatac ttgcatcagc tgcggtgcat gtgagtcgga atgtccagta
     5581 agttgtataa gtcagggcga tgataaatat gtaataaatc cggatgagtg tatcgaatgc
     5641 ggtgcgtgtg ccaatgtatg cccggttgat gctccggtgc aggattaaga gttactagaa
     5701 aaaagaccga aaaggtcttt ttttcgattt agcgaggtgt gtcatggaac agttagtgca
     5761 tagagatgag actgtcgatg atttacagaa cggatttagt ataattcaga aaaaacactc
     5821 tttcagatat ggcgttgatg ctgtgcttct tgcggacttt gccgatataa agcataggca
     5881 ttcggttata gacttgggca ccggaaccgg tataatcccc attcttatat attccaggaa
     5941 aaagcctgtt gagattactg cggtggaaat acagaaggat atggctgaca tggctgcgcg
     6001 cagcatcgta ttaaacggac tggaaaaaaa catcaaggtg ctgtgcatgg atattaagga
     6061 tgcaccaatg cttttgggca agtcaaggta tgattgcgta gtgaccaacc ctccctatat
     6121 gaaaaaggaa tgcggtataa ataacccttc tgaaacaaaa gcaatcgcaa ggtttgaaat
     6181 aatgtgcagc ctggaggaga tattgattgc agcaagggaa ttgctgaagc ccggggggaa
     6241 acttttcatg gtccataggg ccgaccgcct ggcagatata atttatgaga tgagagataa
     6301 aggcattgaa ccaaaacgca taagatttgt acacccgtcc attggcaaaa ggcccaatct
     6361 gttattgatt gaaggagcaa gaggagggaa caaggagctt aagttcatgg atccccttta
     6421 tatacatgat gaaaatggag aatatactga agagatacac agaatatacg ggaggaaagt
     6481 atgattaccg gtattttata tctatgcgca actcccatag gaaaccttga agatattacc
     6541 ctgaggtgcg tcagggtatt aagggaagcg gatatcatag ccgcagagga tacccgtcag
     6601 gcaataaagc tgctgaatca ttttcaaatc cgaaagcccc tgataagtta tcatgaacac
     6661 aataagcatg aaaaaggcaa agagattgtt gagctgttaa gatcagggaa gaatattgct
     6721 ttggtttctg atgcgggtat gcctgcgatt tcagatcccg gagaggagct tgtaaggctt
     6781 tgtattgaca gcggtattga aattattccg ctgccggggc cttctgcttc acttacggca
     6841 ttgattgttt cggggctttc aacaagaaga ttctgttttg aaggctttct tccgtcaaat
     6901 aagaaggaga ggaaggaaag gctcaaaaag ctctccgccg aaacaagaac tataatagtg
     6961 tatgaagcgc ctcatcgttt attggatact ttgggcgaaa tcatggaata ccttggtgac
     7021 aggaggatat ccgtttcaag agagataaca aagaaatttg aagaaaccgt aagaggagaa
     7081 ataagcagcg taatcggtat tttcaaagag cgggctgtaa aaggtgaatt cgtactgatt
     7141 attgaaggca taagtgaaaa agaacttgtt gatattgagc tgaagaaatg ggataatgta
     7201 tctgttgagg agcatattgc aatgtatttg cagcagggag cagacaaaaa agaagcaatt
     7261 aaaatggttg cagaggatag gaagctcccg aaaaaggaag tatataaaat aggatgtaac
     7321 cttgaaagga ggacaaaaaa tgcaattcac aaaggatatg acagtagctc agatattaag
     7381 agcaaatcct aaaactgctg aagttttttt gaggtatggt atgcactgcc ttagctgccc
     7441 gggtgccaca ggagaatctg tggaacaggc tgcaatggtg catgggtttg atgcagacca
     7501 gattttggat gacttgaaca aggtaagtga ataaaataag gggggacgtt tctcttgccc
     7561 cgcaagcggg acaagagaaa cgtcccccct tattcgataa tactattttt taaattcttc
     7621 catgcaagcc ggacaaatgt tcttaccctt gtattgaact acgtcttttg cgtttccgca
     7681 gaatatgcat gccggctcat atttcttcag tatgatatgc tcgccgtcaa cgtatatttc
     7741 taaggagtcc ttctcctcga tattaagggt cctcctcaat tcaatcggga ttacaactct
     7801 gcctaattcg tcgacttttc ttacaattcc tgtagatttc atgtactctc ctccttttac
     7861 attatacaac aaatttcgac aatttctact atgattaaat gttaacattt atgacaataa
     7921 aagtcaatag tgtataaaaa taaattaata attattattt attatctcca acatagctta
     7981 agcccatgcg ctgcaagcct tggggattta tcggaatatt tttcgcgggt atgtaaatga
     8041 tatatattgg tataaaatgt tacaaatcta taaggagtga aaggattaat gggaaaaaat
     8101 accgatacat atcatatcga ttccgatata cctcctgagt cattttggat ggcatcgact
     8161 gccaggacca attatccggc tttgggaaat gatatcaaag tggatgttgc aatagtaggg
     8221 ggcggtatgg caggaataac atcagggtat atgctgaaaa aagaaggctt aagtgtagct
     8281 gtaatcgaag ctgacagaat tattcagggc acgacagggc atacaacggc aaaaattact
     8341 tcccagcacg atctgatata cagcaagata aagaattata tgggagagga aaaagcaagg
     8401 caatatgctg ttgccaatga aacagcaatc cgcgttattt cagatttgat agaacaagag
     8461 aaaatagact gtgattttca taaggagtcg gcttatgtcg ttacattgac ggattcatat
     8521 gtccagcaga tagcagatga agcaaatacg gcaggcagtt tgggaataaa atcatcgttt
     8581 atagaaaata ccccccttcc tttcaaggtt aaaggagctg tgaagtttga cggccaggcg
     8641 cggtttcatc cgcgcaagta tctgctggct ctggcaaaga aggtagacgg aggcggcagt
     8701 tttatatttg aacaaacaag ggttgtagat attaataaag ggaacccctg catccttgtt
     8761 accaacctgg ggaaaaaggt aacagccgat aatgttatta ttgcttctca ttttccgtgc
     8821 tttgacggca tgggctttta tttcgccagg atgtatccgg atagatccta tgctattgcg
     8881 gtaagggcga aggaaaaatt cctcggaggt atgtatatta ctgcggaaga ccccggcagg
     8941 tcactgcgct cccaggagta tgaaggcggt gagcttatta ttgtctcggg ggagcatcac
     9001 aagacagctc atggaacgaa tcttcacagg cattatgaaa acctgttgga atttgcgaag
     9061 aagcactatg atgtgcagga acagcttttc agatggtcaa ctcaggatta caccactctt
     9121 gacaaagtac cctatgtggg aaggctgacc tccaagattc aaaatatata tgtggcaacg
     9181 ggcttcagaa agtggggaat gacaaacggc acagcttcgg caatgatatt gaaggatttg
     9241 attcttaatg gaagaaatcc gtgggctgag gtttatgacc cttcaaggtt tgttttgaat
     9301 ccttcaataa caagctttat atcaataaat gcggatgttg cggccaagtt agcctcagga
     9361 aaactgaagc ctgtgccgga ggatatagag gttgaaaagg gagaggcggc aattgcggat
     9421 attgaaggaa acaaaatggg gatttaccgt gataagaagg gacagcttca tagggtagat
     9481 actacctgta ctcatttggg ctgcgaattg aagtggaatg atgcggaaag gtcctgggac
     9541 tgcccgtgcc atggttccag attcacatat gagggagaaa taattgaagg ccctgcaatt
     9601 agtgaattgc agcatggcag caatgagaag aacaagattg atcctaatat tgtataattt
     9661 ctcattaccc ttgtttccct cctaaagcaa aaagtatgac ccctttttaa aaaaataaag
     9721 gaaatttgta acaggtgtag aagtatatta catgcagttt ttattttata ggaaagtata
     9781 ctttcctata aaatggggga tgcaaggggg agaagccccc tgccgttcaa ggagggtgct
     9841 caggatgcgg agcatccgtc gaagggaacc atagggttcc ctagcgaaaa aaacgcgtcg
     9901 tgagacgcat tttttgccga gggaggtttg gacaatggga gaactgacct tgggacttgt
     9961 tcagatgaga tgcgagaaag gggcaataga gtataacata ataagcatgt ataaatatct
    10021 tgaggaatgc agaaataaag gcgtggatat agtatgtttt cctgaaatga atatcagcgg
    10081 ctgtatcaac ccgctgaaat actctaatgc tctgctttcc atgtttcatc cctcaattca
    10141 gcgtatagcc gatatgagct tcttgtacca gacaatcatt atagcaggtt ttgcagaaaa
    10201 gaactacggg ggaaagccct acataactca gtttgttgca aaagacggca agatagaagg
    10261 ctgctacaga aaaagaactc tgcggggtga tcagtcgaaa tggtttagcc cgggtacgaa
    10321 gcccttcatg tttaggtatt cgggatataa ttttggactg acaataagcc aggatattta
    10381 cgacggcgga atttttaagg agtatgccga gcggggtgcc agtttggttt ttgaaagcgc
    10441 agcacccgga ttatcagtcg gacaaggtgt cggagattgg gagcccgggt tcagtagttg
    10501 gaagaatgaa tgcaagagca agctgggcaa atatgcagca gaggatggaa ttttcatagc
    10561 agcagcaacc caggcaggac gtacatgcga tgaggatttc ccgggaggtg gctatgtatt
    10621 tgctcccgac ggcaggtgca tatatgaaat ggaggatggg gaggaaggca tgcttgtcgc
    10681 caaaatacag ttaaattaaa ccacataaaa caatcatatc cgccatacat tttattatta
    10741 ataaatgtat ggcggtgttt ttttaatgat taataagctc tggttctata tgatagccat
    10801 agctgtcgcc ttcgcagcga caggaagaag gcttgaaatc ctcactgaag agatattttc
    10861 ctctctcggt gctgctgttg aacttggaat tgggcttctg ggaggcatga tgctttggtg
    10921 cggggttttg aaggtggctg aaaaatcagg attggcggat aagatatccg attttatcag
    10981 gcctcttatg aagctgcttt tcagggggat acctcccaaa agtgctgcaa tgggagctat
    11041 gataatgaac ataacctcca acatgcttgg acttgggaat gcggctactc ccatgggact
    11101 taaggcgatg caggagctgc agcagctgaa ccccagaaag gataccgcaa ctaatgcaat
    11161 gtgtcttttt ctggtagtga acgcagcgcc tatacagata ttgccggcaa cggttctatc
    11221 ccttagagcg tccgtaggtt cggcaaatcc gggaattatt atacttccgg cggttgcttc
    11281 gtcctttgca gctgtttctg caggggtgat aacctgcaaa ctgcttgaga aaacatgtgg
    11341 aggcagggca tgaactttat tataccgatt atcatactgc ttatacttat ctacggatat
    11401 ctcaacggag ttaaggtata tgatgcattc attgaggggg cgggggaagg gcttctgata
    11461 accttcagga tactgcctta tataggggca atgcttcttg cggtgggaat gctcaggaat
    11521 tccggcggat tggatttcat gctttatgtt ttgagcccgt tgacctcggc agccggaata
    11581 cctgaagaag tagtgccgct tattattatg aagcccctgt cgggaagcgg cgcattggga
    11641 atccttgcgg atatattgaa aagaagcggc gcggacaact atgcgggagt actggcaagt
    11701 ataataatga gcagtacgga gacgattttt tatacaatta ccgtatattt tggttcaata
    11761 ggaataaaaa atatacgtca cacactggca gcagctgtga ttgcagatat ggcaggattg
    11821 gcagcggcgg tactcctgtg cagattcata ctgttttagc ctccaatatt atatattgac
    11881 actccccaaa tattgttgta tatttttatc atataattaa aactataaaa gtaatgatgg
    11941 gaagagtaag ttggcgtgat tgttaaagag agtcgggaaa ggtgcgagcc ggtacataaa
    12001 accaacccaa catggcccct gaactgcaca ggtgaggtat ttgcttagct tgtgccggta
    12061 agctgacgtt atacagcaca gtgatgaatc ttaaggagga gtcacttgtc gagttttctg
    12121 ccgtgaggca gggaagaatc agggtggtaa cacgagcagc tctcgtccct ttatgggatg
    12181 gagctttttt attttaaagg aggaatttct atgacagaca aaaagacgta ttatatcact
    12241 acgccgatat attatcccag tgataagctg catatcggcc atacttatac tactgttgca
    12301 gctgacgctg cagccaggtt caagaggatg ctgggctatg atgttatgtt tcttaccgga
    12361 actgatgagc atgggcagaa aatccagaga aaagcggagg aaaagggtgc aactcccaag
    12421 caatatgttg atgaagtggt agccggcata aaggaccttt ggaaactgat gcagataagc
    12481 aatgacagat ttataagaac taccgacagg cagcatgagg aagcagtgca gaagatattc
    12541 aaaaaactct atgaccaggg agatatttat aaaagtgaat atgaaggctg gtattgtact
    12601 ccctgcgaat ctttctggac agaaaggcag ttggttgacg gcaaatgccc cgactgcgga
    12661 agagaagtcg agctgacaaa ggaggaaagc tatttcttca agctttccaa gtatcaggac
    12721 agactcataa agtatattga agaaaaccct gattttatac agcctcaaac cagacagaac
    12781 gaaatgctga ataacttttt aaagccggga ttggatgacc tttgcgtctc cagaacctcc
    12841 tttaactggg gaataccggt ttcctttgat gaaaaacatg ttgtatacgt gtggatcgat
    12901 gccttgtcaa actatattac ggcattggga ttcttgagcg atgatgattc ggactacaag
    12961 agatattggc ctgcggatgt tcaccttgta ggaaaagaaa tagtcaggtt ccatacgatt
    13021 atttggccaa tcatgctcat ggctctggga gaacctgttc ccaggcaggt gttcggccat
    13081 ggctggctga tacttgaagg agggaaaatg tcaaaatcaa aggggaatgt tgtggatccg
    13141 gtggtacttg tagaaaaata cggagttgat gcaataagat acttcctgct tagagaagtt
    13201 ccctttggtt ctgacggagt atattctgag gaagcccttg tgaacaggat gaattctgat
    13261 ctggctaatg atctggggaa tctgctttcc agaactatta caatgataga taaatatttt
    13321 gaaggagtca ttccggctgc taagaattcc ggagaaccgc tggatgcaga cctcatcaaa
    13381 ctggctgaaa gtactcctat aaaggttgag gagttaatgg ataagctgct tttctccaac
    13441 gcgttaactg agatatggaa actgatcggg agatccaaca aatatattga tgagacaatg
    13501 ccttgggtgc ttgccagaga tgaatcaaag aaagaaaggc ttgcagaagt tctgtacaat
    13561 cttgcagaag tattaagaat agttggagtt ctgataagcc cctatatgcc caatacccct
    13621 gcaaagatat tccaacagct tggcatagag gatgaagcct tgaagacttg ggaaagcata
    13681 aaaagctttg gggcattaaa ggcaggaacg attgtaaata aaggcgagat aatatttcca
    13741 agggttgagt ttggagggga aaacgctgct ccggaaacac cgaaggaaga taattatatt
    13801 acaatagatg atttcgcaaa gattgacttg agggttgcaa aggtattgga gtgcgagaag
    13861 gttgaaggct ccgacaagct gctgaagctg cagcttgaag tgggaagcga aaaacgtcag
    13921 gtagtgtccg gcatatccaa gtattatgca ccggaagacc tgattggcaa gagcgtagtg
    13981 ctggtggcaa atctcaaacc tgtcaagata aggggagtgg aatccaacgg aatgatactg
    14041 gcagcggatg ccggtgattc tgttgtggta gtttctccgc tttcagaaat accgtcaggt
    14101 tccaaagtac gataatttag caggtacggg gttacgaggt acggggtacg agaaaagtcc
    14161 gagtattccc tttaccccta accctgtttc ctttagtgta ttattaagaa ctaaaaggaa
    14221 gtgagacgta aatgcttttt gacacgcatg ctcatctgga cgatggcagg tttgacaagg
    14281 acagggatga agcaataagc cagtgtaaac aggatggagt tgaactgatt cttaatgcgg
    14341 gaagcaatat tgagacttcg gttaaggcaa tcgcattgtc gaaaaaatat gagttcatat
    14401 atgcttcagt gggggttcat ccccacgatg ccgcgagcat ggatgaagag acggcaggag
    14461 tgcttgctgc ccttgccggg aacaggaaag taagggcgat aggagaaatc ggactggact
    14521 atcactatga tttgtctccc agggatgtgc agaagcagag gtttattgag cagattgata
    14581 ttgcaaggca gctgaagctc ccgattattg tacatgacag ggaagcccac ggggacgtaa
    14641 tggatatatt caaaaaaacc cgtattaaag aggtcggggg agttctccac agcttttccg
    14701 gcagcgcgga aatggcattg gagtgtatta agctggggct gtatatatcc ataagcggcc
    14761 cggtgacttt tgaaaacgca aggaaaacag ttgaggcggt gaaacaggtt cctcttgata
    14821 tgctgctgat cgaaaccgac agtccttatc tgacccctgt gccccacagg ggcaaaagga
    14881 attaccccgg ctatgtcagg tttgtagcgg agaagatagc ggaaataaaa ggcatatcct
    14941 ttgaggaagt agccaggcag accttggaaa acggaaaacg cctcttcgga atagaagaat
    15001 aatgcgacgt aggtaacggg taacacggga gacacggctg tttcatggaa tcgctgcgcg
    15061 attgcacggt aacttttata tattatatag ttacataaga aaatttcgat acttacatca
    15121 ttatcgataa ttatttatgt aacgtaatat ttatcccgcc gtgcatttag cggtttttcg
    15181 tgtcttcagt gttatcataa ctccgaagcc ctgccctaaa cctcgaacct cgcgcctcgt
    15241 acctcgtacc cgaagaatgc gtcgcattct tcctatattg cctaaattcc cctcatatag
    15301 taaaattagc atatatagca aattaagaag ctaatttgca taaacatatg taatgttttt
    15361 catatcgagc ttattttttt attagcaata ttccaaagca ttatcaaacc ttccgggggt
    15421 actgcaatga ttacttatga aatcaacgat gccctttata taaaccttac caacaggtgt
    15481 acaaacaact gcagtttttg tatcagaaat catcccgaag gttttggatt tgatctttgg
    15541 cttccgaagg aacccgttct tgaggaaata atggcggacc tggagaacct ggagctttat
    15601 aatgaagtgg tgttctgcgg ttatggagag ccattggtac gatacaggga aataatcggg
    15661 atatgcaggc atataaagca aaagggagtc agaacaagga taaataccaa cgggcaggca
    15721 aatttaatat ggaacaggaa cattgtacct gagcttaaag gactggtgga ttcaatatct
    15781 ataagcctga atgcaaaaaa tgcggaggac tatgcggagc tttgccggcc ggaatacgga
    15841 aaggcagctt acagcgcagt attggatttt gcgggagaat gtataaaata tattccggaa
    15901 gtatggctta ctgttgttga cgtgatttca gcctacgata ttgaggtatg cagaggcata
    15961 gcagaaggca ttggcgcagg cttcagagtc agacactatt gttaggtgct gttttggtga
    16021 ataaaatgct ttattatggc aaatatatat atgaatgata atattcccca atttaccaga
    16081 gattgtatag atgcttcgtg gaaaaggtaa atttgacaat ggaagcaaat acaggtaaaa
    16141 taaaaatact tgcctgacga ttgaggcaga attggcaagt aaaacggatg ctaaaagtga
    16201 aatcaagaca ggcattcggt taaacaaaaa ggaggttgaa cgatgaaaat aaacttgaag
    16261 gatttcaaga gtttattttc aatcaagctt attgctgtcg ctgcaatatg tttgatattc
    16321 gctgcatctt atgtttacag tgcgatgaaa aaagaaatta ctattgaaga tattggagga
    16381 gtaaaagttg taagtacctt taaggaaacc gtatccgatg tgctggaaga aaataatata
    16441 caactgatac cacaggataa gataaacctg ggtatgacgg aaaagcttga agacggaacg
    16501 cacataaaaa tatccagggc aaagaaggtt acaattagtt cagatggtga ggatattgtt
    16561 atcctgacta ctgctcctga tgtgaaaagt gcattagggg aagttcagat aaagctgggg
    16621 gagaaagaca gggtttcacc cggttttgat actgcaatta cgggagatac aacaattgca
    16681 gtaacaagag tggaagagag aattgtaaag caagagatga aaatggactt taccaatgaa
    16741 gtgagaaaaa gcgacaacct tgacaagggc ttggcacggg ttataaaaaa gggcagccaa
    16801 gggtcaaaag aactgtcaat aaaggttata ttcgagaatg gtaaggaagt taaacgggaa
    16861 atcgcagaag aaaaggtgct taaagagcct gtgaacggca ttattgagga aggaaccaga
    16921 actacctttg tatcttcaag agggcaggtt accagatttg taagagctct aaagatgact
    16981 gcaacagctt atgatgccac ctttgaaagc tgcggcaagc atccggatca ccctcagtac
    17041 ggtattacct attcgggttt aagagttagg cccggtatcg ttgccgttga ccccaaggtg
    17101 attcctctgg ggacatatct ttatgttgaa gggtatgggg aagcccttgc agctgacata
    17161 ggcagtgcta taaaaggaaa caagattgac ctgtattatg aatccccgga agatgtagcg
    17221 aaatatggaa aaagaactgt aaaggtatat gtgcttgata agcccaaata caaattctaa
    17281 aaaatgcgtc gagagacgca tttttattgc gaggtatatc aatgttaaag gaaacaattg
    17341 tggtggaagg caagaacgat gctgctgcag ttaggcgggc agtggaagcg gatataataa
    17401 ttaccagcgg ttttggcata aacggaagta ttctggaaag gataaggact gctcaggata
    17461 ggaacggggt gattgttctt accgatcccg attttgtggg agagaagata cgaaaggcca
    17521 tttccgataa ggtcaaggga gtaaagcatg ctttcctgtc taaggaagag gctgaagcaa
    17581 aaggagatat cggggtggag aatgcagctc ccgaaagtat tttaagagcc ctgtctttgg
    17641 ctaaatacga actggaagag gtccaggcga catttacaag tgaagatttg acatattacg
    17701 gccttatggg aggacaaaac tcagccgaac tgaggaacag ggtaggttct gcattaggta
    17761 taggctattc aaacgcaaag cagttcataa accgtttgaa taagtatggt attagcagag
    17821 aggaacttga agcagctttg gaaaaggtat cggagcaata ctgaaaagga tgatagatat
    17881 gaaggaaaca tcaattaaaa cgtcaacttt agatttaatg aagaagtatg attttaagac
    17941 ggcaaaaaaa ttaggacaga attttttgac ggacgaaact gttttaaaga caattataga
    18001 ctgctcagag ctttcaaagg aggattgcgt tcttgaaata ggccccggac tgggagtgat
    18061 gacgcagata ctctgtgaga aagcaggcag ggtattggcg gtggagatag acaggaagct
    18121 catacagata cttagggttt cgctgttcgg ctatgaaaat gtggagataa tcaatgaaga
    18181 tattttaaag ctggatttga aaaaggtgct tgaagaaaag ttcggcagtt ctcctgtaaa
    18241 gatagtggca aatttgcctt attatattac aactcctata ataatgaagt tgctggagga
    18301 ggatattaac ctcaggagca tcactattat ggttcagaag gaggtagggg agaggatttg
    18361 cgccgccccc gggggaaagg attacggtgc ccttacagtg gcagtacagt accgatgcat
    18421 acctcgcaaa attcttgttg tccctcctga ttcattcatg ccgcagcccg aagttgaatc
    18481 catggtagtg aggctggact tcagggataa gcctcccata actttgaaaa atgaaaaaat
    18541 gtatttcaga gtagtgaagg cttccttcgg tcagcgaagg aagaccctgt tgaatgcttt
    18601 gtccgcaggg aatctgggaa aaacaaaaga tgagcttaag gatatattag caagaaacgg
    18661 cattgatgaa aaaagacgag gtgagacttt gtctctggag gaatttgcga gaattgccaa
    18721 cgacctttta tcctgataaa acagaaaaaa tttgttcact tttaaccccc tcatacatat
    18781 attagtaatg actatgtata gaagggggtt attatgtggc atcaaataat gaatataaaa
    18841 gatattttat aatacttcag gaagatgaca aaggatatga attgtcaccc gggaaaatac
    18901 cgacaggtta tgtaaaaatt gaagtgaaga ataataaggt aagactcaat gcgtttattc
    18961 agaatattaa gcatgaagat cggattgagt atagattgat tcttgtgtca tcggccaaga
    19021 agctggcagt ggatgcagga aagataatta ttgatgggag cgggagagga gagctttcct
    19081 atgaacttga accggataat gttttgaaaa gcggtttgag tattggagat ttcaatgttg
    19141 ccgctgtgac ctgcggatca tccataccgc tttccggtta tgccgggagg gataagctgg
    19201 agtggaagaa taaatatgat atagtaaaca agcctaaggt tcaagaggca ccaaggatag
    19261 ataagaagat agagatcgaa gaaatagttc cggaagtacc gcagccgccg atacatgttc
    19321 cgcttgcccc acagcctgaa ccggctgttg ttgaacctgc cccgaagcct gagccggcac
    19381 ctgtccccat tatggaagct gttcccgaac ccgagcctaa acctgtgcct gaaccggaag
    19441 tcctgataca gcctgctcct gagccggtaa ttatacctga aagcaaggtg gaggttaaaa
    19501 tagtaatacc tgaatgtcct ccaattgctc ctatggaaga accctgcgaa atagaaaagg
    19561 tgaaaccgat aaaagataag tgcgaatatg agaatttaga agaatactat tgttatgatg
    19621 atgaagagcc ctgcaagcac actgcatctc attattacag cggaggaatg tacagcaggc
    19681 ttaagagagt actgagaaag ctgagaagat atgaaccctt tgaaaaagag agaggctaca
    19741 gttggttcaa ggtaggagat gacatgtatg agataaacag ggtatcggtt ccatacatgg
    19801 gttatatgat gcctttgggg tatccatttt tgtctgaagg ctgcagcatg atgatggaca
    19861 ggaaggatta tatactgggt gttagatatg aagacgatga tgaggatgat gacagagggc
    19921 agataaagta tattctcttt ggagtgcctg cgatatatag caaaagaaat gaaatgtatt
    19981 acaaatcaag aggattcatg gaattcaggc ctcataaaac aaaaagctac ggatatttca
    20041 taatgtgtct ggatattagg cggggcatgc tgtgccgtat gaattaaagc aaaaaaataa
    20101 aacaaagttg aattttttat gttagaataa tattaagtat tgaaatacag aagaggaatg
    20161 attttgtgaa agttgagaat cttgtggaca ggaagctcct taagaatata gatttgggca
    20221 tagtaatatc cactatactg cttattgcat acggcatggc agcaatttca agtgctactc
    20281 atattaggag cggaggctct gcgggcacac ttaaggtaca gattattgct tttattctgg
    20341 gaataatcgg agtaatcgtg attctgctca ttgattacaa gaccttcggg gataattatg
    20401 ttcttatcta tataatcaat gtctttcttt tagccctggt attgattatt ggcttttcca
    20461 ccaagggaac caagggctgg attgacttgg gcccggtgaa catgcagcct tctgagatag
    20521 taaagatagg atatatattg acctttgcca agtatctgga aaaaaagaag ggcaacttga
    20581 atagaataat tgatgtatta cctgcaatgc ttcatttggt actgatagta ggcttgataa
    20641 tgctgcagcc ggatgccggc accgcattgg tatttatttt tatttcaatt tttatgctgt
    20701 ttgctgcagg tattagctac aaaataatag caggcgcctt tggtgccttt ttaataagcc
    20761 ttccggtaat gtggaagctt ttgaagccct atcaacagag cagaatactt gtgtttctga
    20821 atcctgaatt ggatcccatg gataaaggct atcatgtaat acagtcgaaa acagctatcg
    20881 gatcgggaca attatggggc aaggggcttt ttgaaggtac tcagaacaat cttggcttcg
    20941 tacttgagag gcatacggat tttatctttt ctgttatcgg ggaggaactg ggactgattg
    21001 gtgctctggt tttgatgatg ctgttcctgt ggctgctttt gagatgcata catattgcaa
    21061 aagtatcaaa ggatgattat ggaatgctta tatgcgtagg aataatatca atgtttctct
    21121 ttcatatatt ggagaacata ggaatgacca tagggttaat gcctgtaaca ggtataccgc
    21181 tgcctttcat aagctatggg ggaagctccc tgctgactaa tatggctgcg gtaggactgg
    21241 tgcttaatgt gggtatgaga agacaggtaa taaggtttta aaagagtata accaaagggg
    21301 tgccgcaaaa ctgcggcatc cttttttcat ataataagaa aattgcgacg gggacacggg
    21361 gacgcgggga cacggggacg tttcccctgt cccgctttca gtgggacacg cgaaacgtcc
    21421 ccgtgtcccc gcgtccccgc gtcctcatgt cgcattattc ttattatatg aatataaatt
    21481 catagaggaa aaaaatttaa taaatattgt aataagtact tgcatattta tgcatacgat
    21541 attataatta taattattac tattattatt actaatacaa tagggggagt ctcaaatatg
    21601 gatattaata agaataaagt agttcttgca tattccggag ggttggatac ctccataata
    21661 attccctggt taaaggaaaa ttacgatttg gacgtaattg catgctgcgt tgatgtaggg
    21721 cagagggatg aactggagcc gataaagcat aaggctctga gcacgggagc ttcgaaggtg
    21781 tatataatag acgcaaaaaa ggaatttgca gaggattaca ttataccaac tgtaaaagcc
    21841 ggagctgtat atgagggtaa atatcttctg gggacagcaa tggcaaggcc gctgattgca
    21901 aagaagctgg tagatgttgc agagaaggaa ggagcaggat atattgctca cggatgcacc
    21961 ggtaaaggaa atgatcaggt acgtttcgaa ctgacaataa aagctttgaa tccggatata
    22021 aaagtcattg ctccatggcg tgagtggact ataaaatcca gagaagaagc agtggattat
    22081 gcggcagcaa gaggaattcc cgttcctgtt tccaaagagc gcccatacag tatggatagg
    22141 aacctttggc atttaagcca tgagggagtg gatttggaag ataccaaaaa tcctcatcct
    22201 acggatattt atatgctgac aacgacacct gaggatgctc cagacaaacc ggagtatgtc
    22261 gaaatcgaat tcaataaggg catacctgtg aagcttaacg gtattgcctg tgattctttg
    22321 gaattaatag aagagcttaa tgtaataggc ggcagaaacg gtataggcct ggtagattta
    22381 gttgagaaca gacttgtagg aatgaagtca aggggagtat atgaaactcc cggcggaacc
    22441 atactttgct ccgcccataa agagctggaa tatataactc tcgacaagca gacaatgcga
    22501 ttcaagcaaa tggtttccga ccagtatgca gatcttgtat ataacggctt atggtatact
    22561 cctttaaagg aggccttgga tgcttttgtt gatcgtactc agcaaaatgt aacaggtgtt
    22621 gtgaaaatga agctttataa gggttcctgt gtaatttccg gaatggactc aatttattct
    22681 ttgtacaaca aggattttgc gaccttcggc gagaatgtag acaatattta taatcaaaaa
    22741 gatgctgaag gctttatcaa cttgttcggc ctgcccatag aggtaatggc gcttatgaaa
    22801 gcaaaactgg ccaagcagca ggaggaaagg aagataggtt aaatgaaact ctggggagga
    22861 aggttcagta agaataccgc acttgaggct gaggatttca actcttcgat ttctgttgat
    22921 tcgaggctgt acctgcagga tatcgagggg agcaaagccc atgcgattat gctaggaaaa
    22981 tgcggaataa tcagtgccga tgaagcagca gaaatactaa gcgggctgga ggaaatcaaa
    23041 caggggattg aatccggaag catagacctg aaaaaggatg ctgaagatat tcacatgaac
    23101 attgaaaagc ttctaatcga aaaaataggt gagacaggta agaagcttca tactgcaaga
    23161 agcagaaatg atcaggtggc tttggatatc aggatgtatc tcagagatga aatccatagt
    23221 atactgaacc tgctgacgga gcttgaggcc ttgctgcttg acaaggcgga agaacatgcc
    23281 ggaaccataa tggcgggata tacccatatg cagaaagcac agcctgtaac cctgggacat
    23341 cattttatgg cttactttga aatgttcaaa cgggatatgg gcagatttaa agactgcatg
    23401 aagaggatgg atataatgcc tttagggagt tgtgcccttg cagggactac atatcccata
    23461 gacagaaaaa tggttgcggg aatcctaaaa ttttcggata ttaccaagaa ttccatagac
    23521 gcggtatcag acagggactt tgcaattgag tttatcagct gttgtgctgt tggaatgatg
    23581 catctcagtc ggttctgcga ggagttgatt atatggaata ccggagaatt cggatatatt
    23641 gagatggatg acggctacag cacaggaagc agcattatgc ctcaaaaaaa gaatcctgat
    23701 attgcagaac ttataagagg caagaccggc agggtatatg gagacctgat tgctcttctt
    23761 accattatga aggggcttcc gctatcctac aacaaggata tgcaggaaga caaggaatcg
    23821 gttttcgacg cgggggacac tttaaagtcc tgcatttcag tatttattcc aatgcttgaa
    23881 gctattataa taaacaaaga aagaataagg aacaatctta taggcggatt tctgaatgcc
    23941 accgatctgg ctgattacct ggttaaaaaa ggattgccct tcagggatgc tcatgaggcg
    24001 gcgggggaaa tggtgaagta ctgtattgcc gccaataagg agctgacaga gctttccatg
    24061 gacgaattca ggaaattttc ctgctgtatt gaagaagaga tatatgagtt tttgaagccg
    24121 gaagtatgtt tgtcaagaag aaacactgaa ggagggcctt ccgaacagag agtcagagag
    24181 gctgtattgc gcggaagaga gtatctgaat cattttgcgg aaacagttaa gttttagcat
    24241 atattggggg aaggtattta tgatttgggt tggaatttta ggggcaaagg gatattttgg
    24301 agaagagctt tcaaagctga ttgccggaca tcagtcggcg caggtgagca ctgtcttgga
    24361 cttgcaggag ctgagtgaat ccggatgcgg atttggcaga agcaatggaa aaagtgcggg
    24421 atacctgaag atgatgaatg cagttaatag gtcggacgtt atatttaacg gattttccgg
    24481 aactgcagca gaggatatat actcgaaagc actttccaac ggaaaaaaaa taatagatgt
    24541 cggtgataag catatagacg gcagtgcata tcccggctca gtatttgggc tgtctgagct
    24601 ttacaaggat aagatgaagg atgcatccat agctgcaaat ccaagcagtt attgcaccgg
    24661 cgcaatgctg ggattggcgc ctctggctgc aggcaatctg attgatataa atactgcggt
    24721 tattgaatcc aagtcgggga tttcctgcct cagatgcgga gataagcttg aatgtgcagg
    24781 tatcaccgct gacgacggca gcaagatata caaaatggag agcagggact atgctgaaga
    24841 agttaagggg cagatgcttg cgctgtttgg taaggacgcc ttatcttcat atgtatcata
    24901 tataattccg ggaatcaaag ggataattac atctataacg gccgaacccg gtaccggatt
    24961 ctgcggaagc gatatttcag agatttacag aggtttttac aaatttaacc ctttaattga
    25021 agttagtggt gctgatacaa taaatggggc aaggaacgga ttcgacaaat atttttgctc
    25081 catatcagcc tcgacagatg cagataccgg taagataaca gtgactacaa ttcttgatga
    25141 tgctgtaaga ggatcagcaa gccaagcggt acagacaatg aaccttatgt atggtatcga
    25201 cggaaaagta ggtttataga taacaaagca agccggcatc ggcttgcttt gttttttcac
    25261 agcatagcag aataataaat tgtacaggct tttaataagc ttgttctttt tgttttatga
    25321 tacttgatgt cgaaaggcca aacaggtata attaattggg tactgcattt tattagccat
    25381 ggaggtggag ttttgggttt taatgtgatt attgcactat ttgccggttt aagtcttttc
    25441 ctttatggta tgcagataat gtcggatgct cttcagaaat ctgccggaga cagattgaaa
    25501 aagctgctgg agattcttac aacgaacaag tatattgggg tcctggtagg tgcggcaatt
    25561 acagcagtta ttcaaagcag cagcgccacc acagtaatgg tggttggcct ggtcaatgca
    25621 gggataatga acctttcgca ggcagtaggg gtaataatgg gggcgaatat aggcactaca
    25681 atgaccgctc agataattgc ctttaagctt accaacatag tcccctatgc cattattatc
    25741 ggtgcaatac ttgtgttatt ctcaaggaaa aaatcacgta agcacctggg tgaattaatt
    25801 atagggtttg gtatactgtt catgggtatg aatatgatgg gtgacgccat gaagcctttg
    25861 aaagacatac ctgaattctc acaattcatg ataggcctgg aggataaccc gcttctgggg
    25921 gttctttccg ggttgctgtt aactgcaata gtccaaagca gcagcgcaac tgtcggtata
    25981 cttcaggttc tggctatgca ggggctggtt cctatcgaag cagcactgcc gatattattt
    26041 ggtgacaata tcggtacatg cgctaccgcc ctaattgcca gcataggtac aggcctgacg
    26101 gctaaaagag cagctataat gcatgtgaca attaagatta taggtacgat agtatttatg
    26161 tttatactgc acccggtaac ttcattagtg gttatgattg cttcagagcc tgcaaggcaa
    26221 atagcaaatg ctcataccct atttaatatt acaaataccg ttattctact gcccttctcc
    26281 gcaatgctta ttaccttcgt gactaaagta ctgccgggag aagatgtgta tgataaattc
    26341 gcattaaagt atctggacaa gagaatactg gagactccct caatagctgt cggacaaatt
    26401 gtaaaagaga ttatcagaat gggagaagtt gcaaagtgca atgtacaaaa aggaatgcag
    26461 gcaattttaa ataatgatga gagagtctta gatgaaatac tgaacaatga gaaagttata
    26521 aatgaattgg aaagaagaat aggtgagttt cttcaggagg taatgcattc cgcaataggg
    26581 gatgaccagc aaaaaatggt agggatgctg ttcaatacag tacatgatat ggagagaata
    26641 ggggatcatg cggataacct tggagaaata gcccaatata agctggataa taaagtttcc
    26701 ttctccaatt atgcaatgga ggaattaaga gaaatatatg aaaatgttga ggaggcaatg
    26761 gacaatgcat ttttggcctt aagcaccaag gatactgctt atgtcgaagc agtagacgct
    26821 tatgagagaa aggttgacga gcttagagat tcatttaggg aaagccatat caacaggata
    26881 aacaaagggg aatgcaatat aaatgcaggg gtgcttttcc tggagatact gaccaatctt
    26941 gaaaggatca gcgaccactg tgttaacgtt gcggatgttg tcaggattgt agacaggtca
    27001 aagctaaagc ttgacagggt gagtgaaata tctcccgaga agtgctgaaa caacggcagt
    27061 catgtgtaag ctgtaaaagc ttgaacatga ctgctatttt tatataatat aggtatataa
    27121 cgaggtgaaa gggagaagaa tttgtatgaa taatcatgat tatctgaggc agataagcat
    27181 cttttcagag cttgagggtg aatttcttga gaaaatctat cagatatcaa gagtccgtaa
    27241 atataaaaag ggcagaataa tattcatgga gggagaaccg ggggaggcct ttttctatat
    27301 aaagtccggc cttgtcaaga tctcgaagct ttcaagagac gggcgggagc atatacttca
    27361 tgtattaaat gaagggcatg tttttgcaga ggtgactctt ttcagcaata cggaatatcc
    27421 tgctacggca gaggttcttg aagatgcaga aataggaata ataaagaacg aggatttgga
    27481 aaaagtcata aaggaaaatc cggacctgtc cttgcagctg atcaagtacc tgaacaaaag
    27541 acttgtagag gcacatatga agattaggaa tctggcgctt tacgatacat atgaaaggac
    27601 tgcccaggct ttggtaaagc ttgccgagga ttacggcaaa aaaagcagta agggggtaaa
    27661 tctggacatt aacatatcaa gacaggagct tgcaaatata gtaggtacta ccagagagac
    27721 agtaataaga gcacttaccg ctttcaaaaa agagcacttg ataggaattg aaaagaatac
    27781 aatcacgata attgacctgg aaagcctcaa ggaatggcct tattaaatta agaataaaac
    27841 aaatcggtat tgaattttga tactcggttt gtttttttat tgtataggaa agtatgcttt
    27901 cctatacaat gggggatgca aggaagagga gtcccctgcc gatgaaggag ggtgctcagc
    27961 tgcagagctg acgccttcgg gaaccatagg gttccctagc gaaaaaaatg cgtcgggagt
    28021 cgtgtttttt atgaaaccag gagctttttt tcgtctaaaa cacaggaggg cattgagggt
    28081 aacgggaata ccggttgcat ataaatttta atattacata tcggtcttag tgtaaaattg
    28141 ttgtaggtca aaaaatagca gagaccactc tttttggtaa aatattagtt gcaaaacaaa
    28201 ataaacacca aggaaggtct cttgtgaata atattataca tcaaatttgt gagaattaca
    28261 ttagtgaagt tttgcatttt ttcgattcag gggcaataag aaaacttgat gaaatcgagg
    28321 ttgttctaaa ggaaaagaca aatagctatc ttaaacaaat gactcaaaca tactttgaag
    28381 agatagatca agctatagca gacgataaga cgggcggaag atcttaaagt agggtttcct
    28441 gaggaaaagc gtagtatcga gggcgttaag aggcttaaaa agcagtgggt atataatcta
    28501 aacagcagga aagcaaacca aaataaatac aaaaaaagag aagaaaaaag agaaaaaaag
    28561 aaaagaaaaa gcataatata tgcactgcga ccgacggagc caggcttctt gtcaaggccg
    28621 cgtgcggagc accgttcatt ttagccttga caaaatggca agcggtacaa taataatatt
    28681 tttctttgtg taatgctgac atctgagaaa atctcaaaga acggatgcaa ggcaacttgt
    28741 gcgtttaccc ggaatactga tttagttaaa ccataaaggg agaatctgct ttttaaaaac
    28801 cgagaacaaa ttgacaccgt cgattgtaac agcgatattg caattttata gaaaaaatgg
    28861 gataatatca ataatgatgc aattttatag aaaaacatgg taatatcatg ttggctaaaa
    28921 aacatttgat aatgtcggat attggagtaa tggcattgga agggtataaa ttaaggtata
    28981 agttaataac gtttctcagt tccggtaata ttaaagatat ttcatctgtt tacgctaaaa
    29041 catttgatat agctttattt atttttccga gtagaaggag ttttattccc tttgatagaa
    29101 tagaagctat catttccgaa accttggaag tctattcaga aaaaaacctg gtgcaggttc
    29161 ctccctttga taaccttgag cctaccatta caaacctggg aaatgtgttt tttgtgctat
    29221 taaaagaagc tctctttcaa aaagatttag ttttagaaac cctggaaatt agtgagagcc
    29281 ctattcgcag ctatatagtc aatgaagtaa atgcagatcg gggattcctt gtcgggcaca
    29341 agaaaattaa agcaaccagt ttgctaatgg atgatataat ttctcaatca acttatcatt
    29401 tgctttcgga aaatgaaaaa gaaggacaga cgccaattcc tgtagttcat gctcccgcgc
    29461 cggtagctga agcagggctt cctttgcctc ctcctaaacc caaaatagag gattcctcac
    29521 ccactttgca gcctgttaag gtaccggttt atcagttcac cctttgtttt cttttattga
    29581 cggtttgtgg agtacttatt acaatatatt taaaaaagac aggagcgtac ccttcgggat
    29641 cagacatata cggtcatctg ttcaaatccg attttcttta ccacagtatc agagcagggg
    29701 acttctatcc tctttatacc gatctttggt acaacggtat gcaacctttc cgctattggg
    29761 caccactgcc ttattatttt ctcgtcatac tccaatttat cgccgggggc gatgcgattt
    29821 cctcatatct gctgttcgta ctttttgctt ttgtgatagg aggaaccgga tggctgttat
    29881 ggggttgggc ctataacagg atgcttttta ctactttcct tggtgtgctg tggttctttc
    29941 ttcctgataa catcagagtt ttctttgttg aaggaaactt cccgcgtatg gcaatagcaa
    30001 tttttctgcc ctatttgttc tattttgtat ggagatatgt ggaacatagc aaaacgtggg
    30061 ctgttgttcc cgtaattttt ataatgtgct gcattatatt atctcatgtt atgattgctg
    30121 ccatgacagg catagggacc ttcatttttt tgtttattta cagtatgaga cagaagcgcc
    30181 tgctcgagtc cttccagata atcgttgcca tgctgttatc atttgcactc tgcggagtct
    30241 ggttataccc ggctcttcaa gggggactga tggggatgaa tgctgctgca acctccgaag
    30301 tcatgcaatc actaagcacc cccatttcaa tatccttgaa tcctttgctg cgtactcagg
    30361 agatatttga ctattattat tttggacttt caatccttgt gattagcatt atcggaattt
    30421 ttgtagctga taaaaaaagc ttacccggct ttcttacatc aattattata tttttgggaa
    30481 caacaactgt tgtagtgcct tttctggaga agctgccttt aaaccagttg ctttggatga
    30541 tgcgttttac acctatcgta tatgccatat ttatgctggc tctactggaa tggcggaatt
    30601 gccgccgcta tgttgcgatt attatagctt tctttcttat tattgacagt attccctctg
    30661 cacatttaga gaaatatttt tcccataacc ctgccagatt atctgattct ctttcaacta
    30721 ccaaagagct tacccggcaa agagtatcat tgatagatct cagtgttttt gattcttatc
    30781 cgtcttatta tttttcaagt aaggaaccga aaaccaaata cacttttgga tgggcatggc
    30841 aaggggctgc gacagcaaac aatattatga tgatcaatac agcagcagag aaaggatact
    30901 attactatct ttttgaccgc agccttgaac tgggtgatga cacagttatg gtgcataaag
    30961 aacttgtgga taaggcaaaa aggacaatga cttcactgct tgaggctgca caggcttcag
    31021 gatacaaatt ttataaagaa acaaacttta cttatatttt tcatagggat actcccgctt
    31081 gttttggtgt tgcttccgaa tatgcggggc tggcaatagg taattccgca aaggcaatag
    31141 cgttggaata tccatctttt gaagaaggtt ccagtgataa cctaacggat tacagatatg
    31201 aggacttaag ccggtataaa gtgatctacc tttccggttt tagttataat gataagaagg
    31261 ccgctgagga gctgctgact cgcaccgcta atgggggcgt caggataatc atcgatatgg
    31321 gtcggatacc ggttgaccct gtgaccagcc ggatgacctt atttggggta acagcccagg
    31381 ctataagttt ttctgctcag tatcccgaat tgatgtatca tgaccaggtt tatcaagcgc
    31441 ttcccttcaa agaagaatac tcgacgtgga atacctttta tttagataat gtagagcaag
    31501 caatcggata ttcctggttt aaaaacaaga atatgccttt tttaggaacg gcgggcagcc
    31561 caaacataat atttatgggt tacaatatat tgtttcatgc aatggaaacc caggatgagt
    31621 caataataat gctgataaat gatttattgg agtttgagcc gacccaactt ccggacaggg
    31681 aagtagtgtc gatttctgtc aagaaacaaa aagacaagtt gataattgat tccgaaaaag
    31741 gtagtattaa tacaacaatt gcttatcagg ataactttcg gagtgatcag aaaatttatg
    31801 aaaaaaataa ccttttagtt gtgcaagagc cccacacgga aattgaaata acctacgcat
    31861 acttggccca aggattggct ttaagcggtg cgggagtact tggtacaggt gttctgctgt
    31921 atttcattat caggaagagg aggtgtgttc aatgaaaaaa ataattcaga aggttcttat
    31981 aggtttatta cttatacagg cggtttcctt ttccggttat gcagactatg gttctattga
    32041 tattgatggt tattatgatg attgggagga taaacctcat accgaggttt attatggtaa
    32101 gaagccttat gtctcggaaa ttcacctggt ttctgtattc cgggatgaaa caacggtata
    32161 tgtgcatata aaaatgtccg agaacgggta tacagaacta ccaaactctt attttactat
    32221 ggatacagac atgggttcag aagcttttac gctgatgctt gattccatga agaaaaaaga
    32281 gtccggtaca gccggaattg aagttcatct gacagataac tgggatgtag tcggttcggg
    32341 gtattatacc agggaggaag gggaatccga tgaagcggag tttgaaatcc cactttactc
    32401 aattacagat gagcctgatg gagtagttga tatcgcaatt atgtttcccg atttgggatc
    32461 gcaggatatt gtatgtgtcg gtgccggaac aggaccatat attgcggttg caataggtgc
    32521 ggttatcgtt ttgctttccc ttgcttatta ttacaaaaag aagcatccgg cttatgccaa
    32581 gtgaaaggaa taatcaatgc tgactttgaa aatcattgcg ttcatagttt ggatatatgt
    32641 tatttgggtt ctcagcagga cgggacttca cttttttaag ttttgcatag gcagtgtcgg
    32701 acttttcttc tttttgatgc tctggataca gcccttaatc atgcagccct taaaaatggc
    32761 agtatctgct gctgctggcg tattgggaga tttttttgaa atgtatgact catattatca
    32821 atactcaata ttatttattc caaaagatac gaccgcagta tcccttacaa ttgattatga
    32881 atgttcaggg gtaattgaaa ttatggcctt cagctgtatg ctatggttct ttccgcttta
    32941 taatacaatt gaaaaactga taatcaattg catagggttg gcagttatat ttttagctaa
    33001 tgttttgaga atttttttga tttgcacact gatttacttt tacggtaatg agattttcta
    33061 tttcgctcat accatttttg gcagaatatt tttctattct atttctattg ctctctactt
    33121 ttatgtattt acccgttcgc atatcatacg gcagaaggta ggtagtttca attatggaaa
    33181 tggagatact gataaataga ataatcagag aagtaatctt ctggatggcc tggataatca
    33241 tccctttaat tatggagatt atccctgctt ttggaggttt ttttatactg ctaaaaaaga
    33301 gctttgtact caaaggtaaa gccattccgc aggctgtgtc ggaaattacc ctgattattc
    33361 cgatatataa ttctgccgaa accttgcgga cttgtttgac ttcagtccac aattccaact
    33421 atcccatcga attgatggat attctgttag tgaataacgg gagcaccgac aatagctatg
    33481 aggtatacag acagtgtcag gatgaattca gagacctgcc gatgacctgg ctcaatgcca
    33541 gtcaaggtaa atccaaagca ttgaatatgg cactatataa cagccacggt aaatatattg
    33601 tccacattga cagtgacgga aaacttcatc ccgatgcact cagaaatatg gcaatacgat
    33661 tcgaacagag gcctgaggta gattgcctta cgggagttat attgacagat atgaagctaa
    33721 tcgaatcgac aaatagtttt atgatgaaat tagtaaggcg atgtgaattt tttgaatatt
    33781 gccagtcttt tttagccgga agaaattttg aatcggaatt caacagtatt tacacgcttt
    33841 ccggagcatt ttcggctttt cgtaaatcag ctattcttaa atcccagctt tttaatacag
    33901 ctacggtaag tgaagatacc caggtaacgt ttcaaatgca caagatacaa aaaaaaggca
    33961 ttagtctttg cgaaaacgct ttcttttatg ttgatcccat tgaaagctat aataagcttt
    34021 atacccagcg ccagcgctgg caaagaggtg agatagaagt atcccatatg tttttgcagg
    34081 aagagctgac tataataaaa ggttttatta gcaattttat ggtgcgtaca ctggtttttg
    34141 accatacatt tgcattcccg cgtatgatat ggtattttgc acttattttc cttgtatttt
    34201 taaactatcc tattgccttg gttttgagct ctataggaat aatatatctg ctatattcct
    34261 tttccatgtt tctttattat atcaatgtac ttatgtatct ggataaggta aaagatattc
    34321 agaggtatta caggtcgaag tggtatctca tatttgtatt gcccgggttt aactttgtgg
    34381 ttttttggat tcgttttgcc ggtattatta acagtatcaa aggggaggga gtgtggaaaa
    34441 ccacatccct gaccgaagaa tggaataaga taaaagttgc cgttaatgac gacttccact
    34501 ttcccaaaag agtgatcgcg gcattaagaa ggaaggttaa tctatgaata cgctgactgt
    34561 ggctttaccc gcctataatg aagaagaaaa tattgaagct ttggtaagca gctggcagca
    34621 gtatcgggaa atacttatgg acaagtatgg gttgatatta cagattgtcg tcatcaatga
    34681 cggcagtaag gataagactg tcgagattgc cgaagatctg aaaagaaaat attcgaactt
    34741 cacattggtc aatcatatcc ataataaagg tcttggagaa gctgtaaaaa cagggattgc
    34801 ttattttctt gacaattgtc ccgaaagccg gtatatgtgt ctgatggact gcgacaacac
    34861 acaggatccg tgttatataa ccgatatgct tgactgcagt aatcgaaccg gagcagatgt
    34921 catcattgct tcccgttatc ggaaaggatc ggaggttatg ggggtatccg gttttcggct
    34981 gattatgagc ggaggggcga gatatgtttt ttcggttctg ctgaatattc caggtgtaag
    35041 ggattatacc tgcggctata ggctttacag cagagaaatc cttcgaaagg cctcatacag
    35101 atttgagaag gatttaattg aggaaagcgg atttacctgc atggctgaac tcttatataa
    35161 gctttttacc tgcggtgcgg tctttggtga aattcccttt gttctgcgct atgactataa
    35221 aaagggaaca agcaaaatgt cagtactcaa aacagctata aacagcatgg gtttagcgct
    35281 gcgactcaga aaaatcaaaa tgacggagaa atcagatgag tgaaattaca gaaaacaaga
    35341 taacagatag caaattgcaa taccgtatag agcttttttc ggctctgatt attacaggta
    35401 ttgtttgtgc tgccggttct tccaatctat tatttgcatc aaatgatttg tatccggcgg
    35461 caacagatgc tatgggtcac atggcaaagg tacaatatat tacccagtgc ctgaagggag
    35521 gggttattcc ttcatggttt ccttattggt ataacggtgc cgccgtcact cagtattatc
    35581 cgccgctttc gtattattta atggtgccgg tatatgccct aacgggaaat gttatgctta
    35641 cattcaaaat caactgcttt ctgatgatgt ttatcggtgg catgggagtt tggtattttt
    35701 gccgtaaggt tatcgggaaa tggtgtggtt gtatagctat tgtattctac tgtttacagc
    35761 cctttctttt gagctcatta tacattgtcg gagtattagc acagggaccc gtctatgcca
    35821 taactccttg gctgctatat ttagtgctta gctttatcaa agaaccaaac cgccagaaag
    35881 cactcttttg caccattgct actgcattgc tgattctatc acatccaatg catgcattca
    35941 taattgttct ttgcataatg ctagtgcttc tgatgtttat tccttctaaa aggatatcct
    36001 tacttaatta tactgtgttg gtagtcatga tgacattttc cttaattata acggcttttt
    36061 ggtctttagt cggagtaacg ggattagaaa atccaacaat tccctatata ttaccaggcg
    36121 tcgcagcaaa tgtcacagcg actcttaaat ggttccaacc cggaaacatg ggtggcctat
    36181 acttctattt tgcactgcca attttggtat ttactgtaat tgcggcaatg gctttaattt
    36241 tgcgcactac aaaaaagcag gttattaatg ctgaaaggtt cgagattcca ttttgtataa
    36301 cattaacatt cttcactact atgttttctt ttggcaggca aattccagga ttcaagcttc
    36361 tacctatgac agatagtttg gtatcgggtc gaattttgtc tcttactgca cttaccggag
    36421 caattgcctg tgcctaccta acctttacag ttatagggta tataccaacc ttgcgaagga
    36481 tgatgcgttt agtaattttg gctattgccc tcagcgcctt tgtcctgaca attgttcaca
    36541 ttaatccata taaattctct tttcccatag ggtcctatga aaaatttgaa aaacggtttg
    36601 aatctgtagc cggagatatt tccgtttctc cttttgacaa aggacgttat atctggttca
    36661 gcggactaag ttctgatgaa gcaaacatac ctatagctta tggttttact atctcagagg
    36721 gatggaatat ggaaggaagt cctcacaacc gtgctttgtg gaatcatcta ttatgtattg
    36781 ccacagatac aagagattat ctagctaaga attttgcttt ctggaatgta agatatttta
    36841 tgttgcctac tcaatatgat gaaatggtaa tacccttaaa agagcatctt ggttttcgat
    36901 tagcttacga cactgagttt tatacaagcc aattgccgtc atcctatttt ctaaccgaca
    36961 accgcaatgg gctgattttt ggtatcggag cacccggagt tgctatggag tttccgtatc
    37021 ttgtctatga acagcgtcgg gatatttctg attattcgct tgcagaactt tcagaataca
    37081 agcttatata tttatgcgag ccggtggtgg ataccatgag gcaaaaagaa aggatcgaag
    37141 aaacaattaa atacctgata gataaaggag tcagaattat tattgaacct acgccaacca
    37201 aaggatttaa actatttgac gtggcagtct ccgatgtgcc tgctgaggac tcactgctac
    37261 tggagaaaca atacatgtcc cctttaaata gtattataga taatgtgaag attgatgaga
    37321 aacatgggta cttcagggca ctgtttggac ttgatgaagc gtattacaaa tcggttcaaa
    37381 atggcggact gttgcaaaat gacattatcg ggacaaaaag agtgggaaaa ggtgaagtgc
    37441 tttttctcgg aatgcatctc agtcagtatc tgaaagctgt atatgcattg aacctgggtg
    37501 tacctgaaga tattaaagga tatccaagct gctcagatga aattaaggct ttattcgggg
    37561 acattttcaa aacctatggg gttgaacaga atttttggcc ggaacctttt cctgtaaata
    37621 atgttcgctg gaattataag ggtgttgatt tcgattattc cagtcaggca gcgcaggaga
    37681 tgactattag tatcacttat acgccacgct ggaaggcgac aatagatgga aaaccggtgg
    37741 atgtcgggca gcgtgaaaac ttgataacac tgcagctgcc tgccggcgag cataaagtgc
    37801 aattaaaata tgggttgaca aaatacgctg tcataggata cactacattg atagcgggat
    37861 tgttactatt ttctcttttt ttaaggaatt atgacataaa tgttattaat ctaagaaaat
    37921 tatgtgcttt attaaagaac tatttgcagg tataaaatca tctttttgtt ttatagaaag
    37981 aagttgagat aaaatgctga aaataggaaa aattagttac aaagttaagc tttttgtcat
    38041 ttccttactg acaataggtt ttatttcgat ttgctggcta tatatgtttc aagagttgaa
    38101 taagtacaag gacagtatta tcaaaaacta tagcagtact caagaaagtc tggttacaga
    38161 ggtctcaaaa aaagttaaag aaagcctttt aaccggctcc ggtgaagaag gtgttattga
    38221 tttattaagt aatgccggaa cttcagggag cagatattgg tttttatata cagcttcagg
    38281 ggcggtattt gaaaaagact atgaagaaac caggaaaatt aaagggaaaa gcgcatcgga
    38341 gttaattaaa ttttggaagc ttcagggagg caccggtacg gaagattttg agaagctgct
    38401 tcttcagaag aaaaatggat cttctgtctt tacaaaaagt gcagattatg gagaagagat
    38461 tgtttcagta aagtatttta ttgcaaataa taccggatac tttttaggaa tgtcaacaca
    38521 acatcagtat gttttgtatg ttggaagggt caatgaacat attctatatt tatatatatt
    38581 tgcaggtgct gtcagcctgg ctcttttagt ccttgctctt tttctgtgcc tgactttgta
    38641 taagggtcag aagcgatgtg ataaagcaaa taaaataata aaagataata acttccacat
    38701 tgaggatctt acaaaaaagc ttgcctcgaa aaccgataca gtgaaaaaag cgagtatatt
    38761 tgacgacctg acaaagcatt ataataggaa gttcttttat aatttgttgt ccaagataaa
    38821 tgatgaacat tttcagccca taagcattgt tgttttggat atcaatggga tggataggct
    38881 aaaccgaaca gcatattaca gaacaggtga tgaggttctt gaaaaaacct ccgggatatt
    38941 gcaaagatta tgtttagata cagatattgt tgccagaacc ggcggcgctg aattcagtgt
    39001 tcttatgaca gttacaaata tatctgaagc ttacgggata acagagaaca taagacgtca
    39061 gtttacaggt cttaatcatg ccgatttgac gttatcaata ggcgttgctc aaatgcaacc
    39121 ggaagataac agcattttta gcgctttgga ccgtgcacgt aaaaacttga ttttggaaaa
    39181 actgaatgat aaaaacagca gcgattatag tattatcgca atgttgatgg aaatactcgg
    39241 agcttattct ctttcaacag ttgcccattg taaccgactg agagaaaaag cactgataat
    39301 cgggaaatcc cttaggttgt ctaattcaga actctcacgt cttgcaatag ccgctcagct
    39361 tcatgatgtg gggaaaatag gtatttccga tacgattttg aacaaaaaag aaatgcttac
    39421 aagacatgag aaagattgta tttacaggca tccggacatt gggtatgaaa taataaaatt
    39481 gattccgcct ttagatgagg ttgctattga tatccggcag catcacgaat tctatgacgg
    39541 caccggttat cccgcaggac ttaaaggaga ggagatttcc ctctgtgcca gaattataaa
    39601 tgttatcgat agcttcgatg cgatgacaaa caaaagtgta tatgctaaag tgaagacgcc
    39661 ggaagaggca attgaggaac ttcagaagaa aagttccatt cagtttgatc cccatatagt
    39721 agatgttttt gttaaagtat ttagcgacaa tataaaggta ggcagtgaga ttgcatgatg
    39781 aatattttaa agcagattct gaaggataaa tttgcattat caaaatattt cttatactca
    39841 gtgatagttg ctgttatcga tactataatt gtttggttac tgacaaggca gaatttgact
    39901 cacttggtta tggcaaacac aataggcgta tcaataggct ttgttcttca ttatttacta
    39961 tcttcaaaat cagtcttcaa aacacgatac agtgttatag ggtttattat ctatttgggg
    40021 actttttttg ttggcttatt tgctgccaac tggcttattt atgtatctta ccaatacatc
    40081 tttgttgatt atatgatgga tttacgtatc ttgctaagca aaggagtctc aataatagtt
    40141 ccgttctttt taatgtattt tttgagaaga tatctgtttt ttattcttaa tagaaaggaa
    40201 gacaaatagt gacatacagt caataccgat tcaaatttta tttcaatgca agtcatgcaa
    40261 tatacctgat ggggcaattg ggggagagtc atcctcacac atgggaattg attatacatg
    40321 gggccaagat aactgacgac tttgcacctt ttaatgagat tgagaagaag atagaagatt
    40381 ttctttcaca atatcaggat gtattcatta atactgtaca gccatttacc acaattaatc
    40441 cgacactgga gaatatatgc actcatttta aaacaagtat tcaagagctg ctttacttaa
    40501 agggatggct tcttctttcc atagaattaa gcgaaacgcc tacaagggca tatgttataa
    40561 gtatgcatga tgaagccgaa tcaaaaaaat catattatga aaaagccggc gcagaaacat
    40621 atcaggatgt tgtggacgag cttgtttttg agaagttgga ttccttattt gaaaacaggg
    40681 aaatagctgc cgaaaaagat actgacggtg aacaaaaata aaaagacata tgaattgcgg
    40741 aaaatgtgac ctgccatttg aattccaatg taaaatgttt gacaggtcac atatagcatt
    40801 aaattttatg atacctgaaa ctaagggtct tttttttcgt ctaatattaa agtagggctc
    40861 gggggttacc gggaacatgt actgtataga aattgtaata ttacatatcg gttactttgc
    40921 tgaaagtacg gggatagtct ggtaaaatag gttatacaat agcctgaaca atggttcaag
    40981 aaatctaaaa taaggtaaaa gtaaatgagg tagtgattat gaaaagaatt cgtggcagta
    41041 gatttttaat taaggcattg gttatactgc ttatagcatc tataacattt ccgggagcgg
    41101 tttattctct ggaatcggcc aaactgattg aagacgggca gcacattggc cccggtacat
    41161 attataggaa tatgaattac ataaccccta acgggaactt tatggtcaac atgatagaga
    41221 gtaaattcga tgacaaatat atcagtgtag aagttgctga cagcggaagc agcctgacca
    41281 acaaaccggt gtcgtatcag gcttcgctga aaaatgatga gaacaagaga gtaataggtg
    41341 cagtcaatgg agatttcttt gacatgacga caataaaagg gcttacttac ggtaccagta
    41401 ttatagaagg agaaatcaag actgcagtaa agacttcaac tatacttgga gttaccgaca
    41461 agggagattg cttcatcgat accttgaata tggaaggggc tgccgcatat aagtacatac
    41521 aatatccggt agactcagta aacaggctca gatgggcgga tcagaccgtt atttacactc
    41581 catttttcgg aaagacaaca gtaaatacag ttgccgggac tgatatcata gtaagaggcg
    41641 ttgaacttcc gcttaaagct gggaagagat atacaggcaa aattgaaaag ataattccgg
    41701 actccaaaat cacagaaata ccttcagacg gagtggttat ttccatgcct gtcaaagcct
    41761 tcaggcagct tgccggagtt ccttccggtg ctgagatgag cttttatgta gattttgaca
    41821 agaaaaacct ggattttgca gtatccgggg cgcccaggct tcttgaaggg gggcagatct
    41881 ctgcgaaggt aaaggaaagg gcagatgcct tgcaaaggca cccaaggact gcgataggca
    41941 taaaggataa caagatttat atggttacgg ttgacggaag gcagcttggg tacagcgacg
    42001 gaatgactct ttatgaattg gtggagtttt tcttgagcca aggagtggaa aatgccatca
    42061 atctcgacgg cggagggtca acggccatgg ctgtaagaaa gcagggtgat gctgctgcaa
    42121 agctggcaaa caacccttct gacggaaggg aaagatatgt gggaaactca atacagcttg
    42181 tatctgaagc accgttatca gatcctgcaa tattgagatt caaggatacc agtgtcaaga
    42241 tatataagaa cagctccttc aagccatcct attacgtttt ggacaagtat tacaatcttc
    42301 tgccgggaga tatgggcaaa gtaaggttcg gaattgatgt gaagacatca aagattgctg
    42361 cagacggcac atacagctcc ggcagccagg caggcaaagg gtatatatat gctgcgtgcg
    42421 gggatacaca aagcaaaatg cttgttgaaa tagtggacaa ggtaagctcg cttgcggtaa
    42481 caaatgattt tgtgcttctt gatccgggag aaaagacaca actgcaggta aaagcctttg
    42541 atgaaaacgg cgggaatatt ataataagcc cctctgctgt caagtggaca gttgaaggtg
    42601 aattgggaac ggtggataaa gatggagttt tcacggcagg aaataataat ggacatggta
    42661 aaataacagc ttctgcagga actgtcacag cagccgttgg ggctaagata ggcaagactc
    42721 ccgtaattgt tgccgacttt ggaacccttg acaacgtgga agcaaaattt atcagaagca
    42781 aggcagctat aagacataat cagaaggggg aaccggtaag gattggaaat atttccttaa
    42841 gacttgatta tgatttccaa aatactgaag gtacgtcagc cgcttatgta acctttaaga
    42901 aaccggtaac aattatagga aagcctattg agcttggagt ctgggtatat ggtgacggaa
    42961 gcagccactg gattagagga aattatataa actcggcggg agagaaaaag gtatttgatc
    43021 tcaccaagtc cggaggcctt gattggaagg gctggaagta tgtatatgct gaattgccga
    43081 aagatgagaa atacccaata gcccttgagc agatatactt ggccgagaca gctcaggata
    43141 ataagaattc cggcagcata tattttgacg atgtaatggc aatatacaaa ctggataaag
    43201 actattacga tcctgtgata gtatcggtac tgtccgaaag cagtgaaggg cttcccggtc
    43261 aggctcagga gataggtgtc tttgcagctg acagaggtta cggcatcgat cctgcttcca
    43321 taaagatgta tattaatgat tctgccgtaa aagccagttt tgaacctgaa acaggaaaaa
    43381 taagctacat tcctgcaaag ccgcttaaaa aaggtgaata caaggtgaga atatcattga
    43441 aggataagat agggcattcc ctgaatccgg aatatgccta taccttcaca gtcaagtaag
    43501 agaagaacga ggacacgggg acgtttcgcg tgtcccactg gaagtgggac aggggaaacg
    43561 tccccgtgtc cttttttcgt aaccttactt ctttaccgct tccagcctgt ttatctttat
    43621 ccccttttta atgaacttct cttcatattc cgtggtgata ttcccgggat aatcctcatt
    43681 gtgaaggtcg taagtcagat caagtatttc aaagccggag gccttgaatt catttataga
    43741 aaaatcaaaa agctctctgc tgtctgtttt gaattctatt cttcctccat ttttcaaaag
    43801 ggttctgtac tttcccagga agagcctgtg agtcagcctt cgcttggcat gcctgacctt
    43861 cggccacgga tcgcagaagt ttatatagaa agtgtccact tcagaaggtt cgaaggcatc
    43921 ctcaatgtcc aaaatgttat aaggtataag aataacgttt tgaagctcca tttcaagcac
    43981 tttactcaga gctgagagaa ttatctcatg ccggaggtct attccaatga aatttatatc
    44041 tctgttaagc aatccatttg ttgatatgaa tcttcccctg cccatgccaa gttctgcgcg
    44101 tatcggagca tcctctttat tgcctgttct ccttgagaag tattcattcc aattgccctt
    44161 gtattccccc ggacggaata tgacgatttc atcatattgc tttataatct caagagcttt
    44221 gggattattt cttattctca tttgtcttct ccacctgcct gccgtagtta tattacaatt
    44281 attatttata gtaagagcat tgtcaatgta tcacacaata cattgctttg tattacaaaa
    44341 ataattatat cagttgttat taaaataaca taaaaagcaa tagacaaaag acattttagt
    44401 atgttacctt aaatataaat agaagaggag gtgttatagt gaaaaaaatg caattgtcca
    44461 ctaaaatctt gattggcctt gctgcaggta tacttgcagg tattttgctt caggggtctc
    44521 ctgacgtggc caatgcctgg atcaaaccct tcggtacctt gtttctgaac cttatcaaaa
    44581 tgataattgt acctttggtg cttgcatctc ttgtagtagg aacttgcgga ctgggggatg
    44641 taaagaaact gggaagaatc ggcggaaaaa ccatagccta ttatctgtta accactgcgt
    44701 ttgcagtaat tttaggactt attcttgcta atatattcag tgtaggtgca ggttacgttc
    44761 ttccggcaga tgtaaaagca gcggaaccca gcaaaatacc aagcttcgtt gacacattgc
    44821 ttaacataat acctaccaat ccgataaagg ctgccgctga cggcaatatg ctccagataa
    44881 tcgcatttgc attgatactg ggcagcggaa tagtagtatt gggagataaa ggcaaaccgg
    44941 tcttagactt ctttgactcc cttgcagaaa tctcatataa gattacaggc ggaatcatga
    45001 tccttgctcc ttatgctgta tttgccctca ttactccggt tgttgcttca aacggaccta
    45061 aggtgctgct gccgctcttg agtgtaatca ttattgttta tgtaggctgc gcactccatg
    45121 ctgctctggt atactccggc acactgatgc tatttgcgaa gttcagtccc atgaagttct
    45181 tcaaaggaat tttccctgct caggctattg cgtttacaac ctgcagcagc agtgcaaccc
    45241 tgccggtgaa tatgcagtgt actgaggaga atcttggtgt tccaaagggt ataagcagct
    45301 ttgtattacc tttaggtgca actataaaca tggatggtac tgctttatat caaggcatat
    45361 gcgcactttt tatcgctaac gtatacggtt tggagcttac catggcccag cagttcacta
    45421 ttgtattgac tgctaccctt gcttccattg gaacagctgg tgttcccgga gcggggctta
    45481 taatgcttac aatggtgctg cagactgtag gactgcctct tgaaggcctg gcactggttg
    45541 ccggtattga ccgaatactg gatatggcca gaacaacagt taacattaca ggagactcag
    45601 catgcgcagt tatcatagca gcatccgaga atgagctgga cagagactgc cagataaagc
    45661 atacttattc ggcataaatc aaaaaagcgg cgaaaagccg ctttctttta ctttatcttc
    45721 cgataaacaa ctgcgtatag atataacttc cattaccctt tggtgcaatt ccaacaccca
    45781 attcggtaaa gttgggattc agtatgtttt tcctatggcc ttccgagttc atccacgcct
    45841 tgaaggctgc ttcggcggac tggttcattg caatattctc tcctgcgtac atatagctta
    45901 cattattaga tttaagcata tcaaagggtg agccgtaagt cggggactga tggctgaagt
    45961 aattgttatc tgccatatcc tgtgacttca tccttgccag tcttgtgcag tcactgcttg
    46021 cactgagagc ctttgcaccg gcttttgctc tttcagcatt aacaagctgc agtattctag
    46081 attcatcgga actcatggca cctgatcctg ctgctgtccc tccgccagta gtgcctgctt
    46141 cggtcgtacc cgcacctgca cctgctcctg ttatagcacc ttgattagga gaaggagtaa
    46201 cagtcccggc gcctgtagtg ccggtatttg cagaagtact gtaaggcctt gcacttgcag
    46261 acggtataca gccgattctg ccgctgtcgg gcatctttac tacataccag ccatccagtt
    46321 ttccgagggc tctgattttt tgtcctgacc ttaaagtgcc gacggatggg aatttgtttc
    46381 cggctcccgc tcttacattc aggttatttg tattgcatgt aatgtagctg aaatccacta
    46441 cctcataagt agcggatatt ttgttgccgt cagcgttatt atctgccctt ccgaccacat
    46501 tgttcggaat ggccggagta ttcggtgcag ccatgttatt ggtatttggg tcggtttgga
    46561 tgtatcttct tgccgggtca taggtacagc cgccaagaga ggaaacaagc aaaagtaata
    46621 ttgcagcttc tgaaaatagg gctttgtatt ttttcataac taattccctc ctaaggtgaa
    46681 tttagaattg aatattattt atttatatat atattttgac tcaaacacaa taaaatattg
    46741 actctaattc tgccaatttt tgcagagaca aaaaaatgca ggaagaaagc agaaagatgt
    46801 agaattaata attatattgc tgaatgttat ttaacagatt atacaggggg aaaaagtgtg
    46861 agtattctaa tacaggcttt attaatattt tttgcaagaa ttattgatgt atcaatagga
    46921 accatgagaa ccatattgct tgttaaaggt cagagaagaa ttgcttcggt attgggtttt
    46981 tttgaagtaa tgatttatct tattgtgttg ggtaaggtag taggaaatat tgataaaccg
    47041 atgcttatac tggcatattg ttccggttat gctgccggca atataatcgg aagcaagata
    47101 gaagagagat tgtccattgg gcgtcttgtt gtccaggtaa tagtgaagga agccttggag
    47161 gagttggtag agagtctgag ggaagcaggt tttggagtca ctattattga gggcgaaggc
    47221 aaggaaggga aaagctatat gctgaatata atactggaca gaaaacaggt gaaaacgctt
    47281 tatgaaatag tcgacggatg tgactgcgga gcatttatta ccacaatgga tgtaagatcc
    47341 tctttaggtg gatatttcca gcctaagaag atgtagattg gcttttggcc cactgaaatt
    47401 cagaaataat cattgcagat actataagta tgccgccgat aattcccctt gcaccaagca
    47461 cttcgttcag cagaaggaaa gaggtcagtg ctccgaatac cggctccatt gtaaaaatca
    47521 gggcaatgtg ggatgcggcg gtaaatttct gagctgtagt ctgcatccag aacgcaaaag
    47581 ccgtgcagaa taccgccgag aacattaaag accagaaact ggggacagac atatttgccg
    47641 gaatgggttc aagaataacg gcaaaggcca ttccgtacaa tgatacgaag aataactgaa
    47701 caatggcaag caatgtcgcg tcatgcttct gtacataggt tccggtgaat aggagctgcc
    47761 aggcataaca tatcgaacat aatattgtta gaaaatcccc aatgttaaag ttaaaatcgc
    47821 tgcctgtata agatagcaaa aacaggccca gggtagccgt agctacgctt attattgttt
    47881 tcagctccag ctttgttttc aggtagaata ttgagaaaat aggcactata agcactgcaa
    47941 ggcctgagaa gaagcctgat ttcgaagctg tagtatactg tatgcctaca gtttggaaag
    48001 cataggttgc aaaaagcaat gatgacagta tcaacgcagg ttttataagg cttttattta
    48061 agcttcgcag tttcctgtaa aacagcaggg aaagaagcag actcgccaca gcgaatctga
    48121 tcccgatatg ataaaagggg gtcatggttt ggagagcaat ttttgttgca gggaagcctg
    48181 ctccccagaa tatagttaca agaatcaaag ccaggtctgc tttcagggat tttttcatgg
    48241 tctgttctct cctaagctat gtttaaatta ttatgcataa tctattgtat agcatataat
    48301 aacaaatatc aattaatgca tttattgtat actttttatt gacacagtaa tactatgact
    48361 atataattaa tttatctata tttctaacag taatgatgag gataagtaat ctgcggaggc
    48421 ttacgtgcat atgcatcaga tgcatggcac ggccagagag ccggcggtag gtgcaagccg
    48481 gcaactgaag cttatgaaat acacctctga actgcgggtt gaaagcattg caagtaggcc
    48541 tttgccggaa gcttcctccg ttatgacagg gaagggaata tgtaagtatt ccagagtggg
    48601 ttttgcaggt tttatatctg cagggccaat tagggtggta ccgcgagcct ttcgtccctt
    48661 tgtggacgaa gggcctttat taattttggg aggtgtttat tatgacaatg gtaccaaaat
    48721 ataatgtata tgatgtatta aaagaaagag gctttattgc gcaggtcaat catgaagaag
    48781 aattgagaga gctgttgggg aaagagtgta taacatttta tataggattt gatcctactg
    48841 cagacagtct tcacgtcggc catttcctgc agataatggt tatggctcat atgcagaaag
    48901 ccggccacag gccgatagct cttttgggtg ccggaacagc aatggtgggg gatccaagcg
    48961 gaaaaagcga tatgagaaaa atgctgacct ttgaaatgat tgatcataat gcaagaaact
    49021 tcaggaagca atttgagaag ttcctggact tcagaaatga cagggctatt atggataaca
    49081 atgccaactg gctgttaaag cttaactatg ttgacttttt gagagatata ggagttcatt
    49141 tttcagtcaa cagaatgttg agtgcagaat gctataagag caggtacgat aaaggactta
    49201 ccttcctgga attcaattac atgcttatgc agtcctatga ttttctggag ttgtacagaa
    49261 agtacggctg caggcttcag ttaggcggag atgaccagtg gtccaacata ataagcggaa
    49321 ctgacattat aagaagagtt gagggggctg acgcctacgg tatgaccttt acactgctga
    49381 ccaccagcga agggaaaaaa atgggaaaga cagaatcagg ggcaatatgg ctggatgaat
    49441 caaaaaccag tccctatgaa ttctatcagt actggagaaa cgttgatgat gccgatgttg
    49501 aaaaatgcct tgcactgctg accttcctgc ccatggatga agtaagaaga ttgggcagcc
    49561 tgcccggtga aaggataaat gaagcgaaaa aagttcttgc ctatgagctg accaggatgg
    49621 ttcacggaga aaaagaggcg gcaaaggctc agaacacggc agaatcgctg tttggaggaa
    49681 gcggagactc tgaacatata ccaacaactg aattgtcaaa ggaacaattg gataagggtg
    49741 taaacatagt agatcttttg gttatgaccg gtcttgcccc ttccaaagga gagggcagga
    49801 ggcttataaa ccagggtgga gtattagttg aggataccag agttgagagc atagatctga
    49861 ttataggaac tgcaaatctt aaagacggca agcttatcat aaaaaaaggc aaaaagacat
    49921 atcataggat aaaaataaaa gaatgatgcg tcacgataca tggggaggac acggggaggg
    49981 acacggggac gtttctcctg tcccgctttc agcgggacag gagaaacgtc cccgtgtccc
    50041 tcctcgcaac ctgaagaaaa ttgctgtgca attttcttca attatgatga agttttttat
    50101 ccagtgcata aaaactgcct ttgactattg aatcgatttt tttctgcata tttttgattt
    50161 gaatataggt ctccgggtat gcagcttttg aaacatcaat ctcaccttcg cctttggttt
    50221 caagaatccg ctccagcttt ttcctatatt catccagcag ctttatttca tccagaatat
    50281 tttcgtattt tctgatacat tgatggtatt ccctgctgtt tacgtattca gctcccggaa
    50341 tattgatttc aaaggattcg atttgttttt ttgccttggc agcttcattc agagtaacac
    50401 tgtatttttg ggagagttgt tcaatctcct tccgaatagc ttcccggtta aaacctgaga
    50461 cggatatata cgtttttttc tctgatctgt ttccaattat accggtaaca acttggattt
    50521 cagcatttac ggaacctccg ataattttac cgtgcatagg gtccaaaatc actttttttg
    50581 cttcgagatg gctgtcaaga gaataaaaac caatattgat atcttctcct gccacaattt
    50641 tgcattcgtt gcagtacttt ataaatacac tcttttttgc ctgtatttca gatatgttct
    50701 taccgaaaat accacctttt atatatatgc ttccctcttt tgagattatt ttatttatta
    50761 ctcccaagcc catgttgcct tgtattgata tatcattttt agctgttaca gagaaaccgt
    50821 cctttactat acctttaacc gtaacatatc cttcaaagga tatgttaccc gtttcatagt
    50881 ctacgtcctt tgttattatg aggtgattat ccacctttat cttgtcattc tcaaatttta
    50941 cggcaccgtc tgttttagcc ctgagtacga ttttgcctcc ttcttcatgc tcttcaacgg
    51001 ttttcttatc atatttaagt gttaagtcaa tcccttttcg ggcaggcaat actttccccg
    51061 tgacggttat gccgggtttt ccttccgtgg caggtatttt ctcccccagc cagtcacctt
    51121 ttttgacatt atcaatcaga ttaagctcat aatggtttac agttccgtct tcttttacaa
    51181 tgggcttctt atcggggatt tcatagtact tgattacagt atccttgccg tcttccggtt
    51241 tggtgccctt tgcggcttta atttttttct gaacggtaat aggttttttg aaaatgttgt
    51301 catgaccgaa aacaaccccg gcattattaa gcgcctcaat tatttccgaa gaaatcggaa
    51361 ctttgttttc ggcaaattcc tttgccgaaa tattgagcat tattgatgct tccatttcgt
    51421 catcagatat ttccacttcc acccttggct tgatttttcc tatacatatc ctgagcctgg
    51481 aagcctcatc caatgcattt ttcagggttg taaaactatt gagctgtaaa aatggcatat
    51541 ccataaggag agagttgaat tcgcgaatct cgtacccggt gtgatataca caaatataca
    51601 gctccccatt aacgctgaaa agcgaaaaat attcgttatg aaatatttcc ataatattac
    51661 ctcgatttac acattttata acaattctat catgctgttt gcgaataaac aagttttttg
    51721 ccaataaagt atgtaaaaaa ttgctcccta aattgctctt tttgcgtatt ttagcaaaat
    51781 ctaaactaaa gacatagatt atggatataa aagagcaaaa gttgaaatat taagcttaaa
    51841 cagaaacact tgtattatta gcgcgaatga ctctcatttc taaatgctgc ataataatat
    51901 aattattatt aaatagtttg aaggtgatat atatggataa aaatatagac gtatatataa
    51961 gaaggcttat agaactcgac tcaagggctg tggagcttaa gcgggaaagg gatgcagaga
    52021 tttcggaact ggaaatgagg ggcgaaaatg agcttaaggg cttggatgaa gagttggagg
    52081 aagccgttat taaggcaaag cagagacgag atgagataat tgaggccggc aggcttcagg
    52141 caaaagagat ggaggaagcc gtaaatttaa agattgataa gcttcagacc cattttgaaa
    52201 acttcaaaga agatgctgca agagatattt ggaagcagct tcttgcaatt gagaggtaag
    52261 tttgatgggt aatatcacca ggtttgctgc tgtgaatact aagataaaat caatggaggg
    52321 ggaactccta aaggatgagg attatacctt attgcttgga ttggaatcgg taacagaagt
    52381 agccggatat cttaaagaaa ggactgccta ttctgaagtt cttgcagaag tggacacggg
    52441 aactatccat agaggcatgc tggagaacct gataaagcag aagatggtaa agagcattga
    52501 caggataatt cattacttca caggggatta taagagcttt gtcaaaaccc tctatgcaaa
    52561 atatgaaatc gaggaattaa agattttagc aagagcagta tataacggaa aggatgcagc
    52621 tgctttcagg aacaatgtct ttgtagggaa gcacagcagg attgatacaa gcaagattta
    52681 tgaggctaag tatataagag atatcatata tgcacttcaa ggctctgagt tctataagtt
    52741 cttgatacct ttggttgacg gagatttttc cgagaatcct ttcagattcg agatggtcat
    52801 ggatatgtct tactactcta tactgcagaa caaatggagc aagcttgaaa agcgggatat
    52861 aaaggcattg gagcaagctc agggaattat tgccgacctg ctgaatattc agtggatata
    52921 taggggtatt aagttttata ggcttagccc ggaagagctg ttgaactata ccattaatat
    52981 cggttacagg atggatttta atatgctgaa gactttatgc tattcaaaaa gtctggagga
    53041 attcaacaga attgtaaaat ccaccaggta tggttttatg ctgaagcatg acggaaccac
    53101 tgatatttat atggaaagga ggatggagcg ctatatatgt tttgaactca aatccatgat
    53161 gaaaaataat gctctttcta ttattagtgc ttttgcattc ataatatttc tgcagtttga
    53221 ggtaaaggac ataatatcga taattgaagc cataaggtat aaagtaccgg cggatcaagc
    53281 ctataaatat atcgtaagga agatctgata ggagatgaaa atcattggct gttgagagaa
    53341 tgaaaattac tagtataata ggaaagctta cagacctgga caaagtatca agactaattg
    53401 tgataaacgg cagcactcat atactgaatg ctatttcaga gttgaattcc aataacctga
    53461 acctgtcagt tccgcagaag catatgcaga ttcttcaaga attggtaaat atcaagccgt
    53521 atatttccgg aagggacttt tccggggacg aaggaataat aagattattt gaggacttat
    53581 tcaatctcaa gctgaagata tgcgaggatt atttatgtgt tggcgatgag tatgaaaata
    53641 ctatggcaga attgaggaag gtgcatgaag atgtcaggca tatttccgaa gacatggcag
    53701 agagagcagc taatattgag aagctgcagc aatatctgaa taatgtgagt tatcttagga
    53761 atactgattt aaggctggaa gaaataaata atttaaattt tatgaaattc agattgttaa
    53821 tgctttctaa tgagaatttc aaaaagctta agctaaatat tgaaaatgtg cccgcaataa
    53881 tatttcaggc agcagttttt aacggaaata cagtaattgc ttcgatcaca cccaagagcc
    53941 tggaggaaga ggcggaaaga atttttatat cattgaatta taaggaactg gagttgcccc
    54001 aggaattcag cggaaccata gaggatgtga taaatcagat aaatgcgaag agcagtaatg
    54061 aaaaaagaca ggttgaggaa cttagagaat ccgtagcaga gatgagccga acgtacagtg
    54121 atactgtgtt caaggcatat acactgctgg aacttgaaaa aaaggcggaa aagctaaagc
    54181 aggaaacagc tttgggcagc agcatgtttt ttgtgttcgg ctttgtgcct gaaagtgaag
    54241 ttgaaggtgt ccgcattgaa ctgaagcaat atttcggcga aagtattata tttatagctg
    54301 acgatgtaga ggataaaagg ttgggaaatg ctcctccgac taaaatgaag aatatttcat
    54361 tattcaaacc ctttgagtca ctgataagca tgtatgggat gcccagctac ggtgagaagg
    54421 atcctacacc gttttttgcc ttgtcatata tgctcatgtt tggagcaatg tttggagata
    54481 tgggacaagg actgataatt cttttggcag gcttgctgat aagttataga acagcaaata
    54541 agagctttgg agggatactg acaagactgg gagtcagttc catgattttt ggagcattat
    54601 acggaagcat tttcgggtcg gaggaattga ttggggcact gcttataagg cctatggcaa
    54661 atataaatac aatgctcata ggtgcaatag tattgggaat catactgata accataagct
    54721 atatgtacag cctgctgaat cattacagct ccgggaatat agaagaaggg cttttcggca
    54781 gggaaggcct gacgggtttc ctgtttttcc tgactctggt attcggagca ttgggaaagg
    54841 ccacagggga actgactgta gggataggcc ttccaatagg tattatggtt gtgcttctgc
    54901 ttataatggt gtttaaacaa ccgcttgcag gcatgcttac aggccgcagg ctatatgagg
    54961 attctccggg ggattattat atagaagcag gcttcggggt tatagagaca attctttccg
    55021 ttgcatccaa tataatatct ttcataaggg taggagcttt cgctcttaat catgtgggat
    55081 tgtatatagc ctttgccgcc atggctgaga tgctgaactc aaaaacagca ggaataatgg
    55141 ttcttattat tggaaatatc tttattattg gcttggaagg acttatagtg tttatacaaa
    55201 gtctcaggct tgagtactat gagctcttca gcaaatacta ttccggctac ggagttgaat
    55261 acaagccggt tggattgttc ggaaaaggag actccggaaa tgagatggta tgattccggt
    55321 cagatgaatt caaatataaa acaaatatag acagggggca tataaatgaa tacaatattg
    55381 ggaattgcaa tagggattgt catatttaca ataggtctgg gagtcgtatt ataccgttcg
    55441 gcagccaata ttcaaggcag aaagatcaga aacattttaa gagccagcct ggtttcttat
    55501 atgtttctaa tagctttgtt tctcttcatt cttactcctg acatcgtaaa tgctgcacag
    55561 gaaggtgatt ccggcgcagg catgggcttc atagctgcag gtatatccac cggccttgcc
    55621 acaatcggag ccggttatgc agtcggtgca gtgggttcat cagctttggg agcagtttca
    55681 gagaacccga ggatactggg gaaaacatta atattcgtag gcctggctga aggtatagca
    55741 atttatggac ttattgtatc aataatgata ttgggagcac tttagaacgg cttgctctca
    55801 gccattttaa agaggagaac ggcgatgaga tttttcttat taagcgacaa tatagatact
    55861 ttgacgggaa tgaggcttgc aggaataaat ggtgtgttgg ttcatgaaag agaagagata
    55921 cttgctgctt ttgatgaagc atgcagcagt aatgatatcg gaataatact aatgacggag
    55981 ctgttggcag aaaaggttcc ggaggaactt aaagctataa ggctggacgc gtccaagcct
    56041 ataattacta ttattcccga caggcatgga gaaaggcgca gaagagatta tataacaagc
    56101 tacattaaag agtccatagg gctgaagatt tgaggtgatg agtcttgtca gtatcggttg
    56161 aagataagat agagcttttc agaaacataa tattcaagga tatagaagag actgcttatg
    56221 atataaggca taaagctgcg gaaggcttta atcagaataa ggacagactt ttgcaggaag
    56281 ttgagattag aaagaatcag ataatgcagg aagcggagaa aaaggcagag aaagagaaac
    56341 agcagcttat agcaaagaca aaatctcagg ggcagcatcg tttgcttgaa aaaaggcagc
    56401 agtccataaa tgaaataaca gagatgttga ttcggaaagc cgacagcttt gtatccgagg
    56461 agggctacaa ggaatatctg gcagcaagtc tgaagaaggc tgccgtcgat tttgaaaatg
    56521 ccgcttcagt acaatttgct tttacaaaga gggatttgag aaccttaaag ggatttatcg
    56581 aacaggaaat agcctccgga gaattgaagg acagatgtca gcttagagaa tcagaaaaga
    56641 atataatagg gagcttttat gcggaggatg aaaaacggga aatacaggcg gattatacat
    56701 taagatcctt gattgaggaa aatcgtgagc tgattgggag cagcatatac cgcaggcttg
    56761 atgaggtgta aggcaatgga cactaataaa ggaaaaattg tatatattaa cggccctgta
    56821 gttaaggttg ctgaggctaa cgaccttaag atgagggaaa tggtgctggt aggacgcagg
    56881 cagctgatcg gagaagtgat ttctgttgat aaggagacag ccacaataca ggtatatgag
    56941 gagaccacag gacttatgag aggagaaggt ataatctcca ccggaaggcc tttatcggta
    57001 aaactggggc ccggaataat aaagtatatt tttgacggta tagagcgtcc gcttcttagt
    57061 atgtacgaag caggcacagc attcatacct gaaggaatcg gtatgattac aatagatgaa
    57121 gaaaaactgt gggatgcgga aataattgta ggaatcggag attaccttga ctcaggggac
    57181 atttatgcaa aagtacagga gacatcgctt attgagcaca ggcttatggt tcctcccggc
    57241 attagcggga gggtggtttt tgcagaaaag agcggcagct ataatattga gcacgtgctt
    57301 gtaaagctgg aaacggagac gggacttcac gagctgaaaa tgtatcagat gtggcctgta
    57361 agaggtgccc ggcctgtcag ccgcaggaat cccatagcaa aaccgctgat tacgggccag
    57421 aggataatag actctttctt tccattggca aagggcggca cagcagccat tcccggaggc
    57481 ttcggaacag gtaagacgat aactcagcac cagcttgcaa aatggagtga tgccgatatt
    57541 attgtctata ttggtgtcgg agaaagagga aatgaaatga cagaagtgct cgaggacttt
    57601 ccgaagataa tcgatcctaa aagcggaaaa tcaattatgg agaggacagt actgatagca
    57661 aatacctcca acatgcctgt ggcagccagg gaagcgtcaa tttataccgg aataactata
    57721 gctgaatatt tccgtgatat gggctacaac atagcaataa tggcagactc tacctccaga
    57781 tgggctgaag ccttaagaga gatttccgga aggcttgaag agatgcctgc tgaagaagga
    57841 tatcctgctt atcttccctc aagacttgcc caattctatg aaagagccgg agatgtggaa
    57901 gccttaggcg gcagagacgg ttccattaca ataatagggg ccgtgtcacc ggcgggagga
    57961 gatttctcgg aaccggttac tcagaatacc aagagggttg taagcacttt ccttgctctg
    58021 gataagtcac tggcttatgc cagacactat cctgccatca actggttgaa gtcatacagc
    58081 ggatatatca ctgacctgag tttatggtat gaagaaaaca ttgctccgga tatgatggaa
    58141 cttagggcaa agctattgag gctgcttcag gaagaggaaa agctcatgga aatagtcaag
    58201 ctggtgggag aggacatatt gcccgatgac cagcggttga tactggaaat ttccagggtt
    58261 atcaaggtag gctatcttca gcagaatgcc tatcataaga atgatgccca tgtatccctg
    58321 aaaaagcagt atggaatgct cagggtaata gacaggctgt atgataaagc ccataactgt
    58381 gtaaaaatgg gtgctccaat atccaaggtc aagaatgatg agttgtttta caagattaca
    58441 actatgaaat ataatgttcc ggaagacaaa ccggagatgt tcgatgaatt gataaatgag
    58501 attgataact tctatgacag cctgaaaacc atgtacggca gagaggtgag cagacaatga
    58561 gattaaaata tttgaagctg caaaaggtag aagggcctct tataattctc agcggggcca
    58621 aggaggctgt atttggagaa gtcgttgaca ttgaggtgga gcaggaagga cataagaaag
    58681 ggaaggtcgt acaaattgac ggagacaaag ttataataca ggtttttgaa agtacttccg
    58741 gtatatcact caataatgcc agtgtttcct ttacgggaaa accgatggag ataccactgt
    58801 caagggatat actgggaaga acctttaatg gcataggtga gcctatagat ggagcaggct
    58861 tttatgcgga cagtaagtat gatgtcaacg ggcgccctat taatcccgta gggagaagat
    58921 acccgcgaaa ctatatagaa acaggaatat cttcaataga cggccttgct actcttgtaa
    58981 ggggacagaa gctgccgctg ttttcaggtg acggattgcc ccataatgaa cttgcggcac
    59041 aaatagttaa gcaggcaaag atatcagggg atgatgagag caattttgcc gttgtatttg
    59101 ctgcgatggg gataaaacat gatgaggctg attttttcag aaagcagttt gattcgtcag
    59161 gagttgcaag cagggtggta atgtatataa acctggcaga taatcccatt gtagaaagaa
    59221 ttactgcgcc cagatgcgcg ctgactgcag cagaacacct ggcatttgtt cataatatgc
    59281 acatactggt aataatgacc aatatgacca gctacagtga agctttaaga gagctttcgg
    59341 catccagaga agaggtgcct tcaaggaaag gctatccggg atatctgtat tctgatttgg
    59401 caagccttta tgagcgggca ggaatgataa agggttcgaa gggcagcata actcagatac
    59461 cgatacttac aatgcccaac gatgacataa ctcatccggt gccggatctt accgggttta
    59521 ttacagaagg gcagattata ttatcgagag aacttcacca aaaagcaata ttccctccca
    59581 taaatgtgct tccgtcgttg tccaggctgc aaaaggaaag cataggtgaa ggctttacca
    59641 gggaggacca tcctgatgtc gccaatcagg tattttcggc atattctcat gttcaggacg
    59701 taaggtcact ggcccaggta ataggtgaag atgacatttc tgaaatggat aaaagatata
    59761 tggagttcgg gagacaattt gaacaaaagt tcctgaacca aggctctgag gtgaacagaa
    59821 gcatggagga taccctggat atcatgtggg agcttttgac atttcttcca aaatcggagc
    59881 ttgacaggat caatccggaa ctgcttgaca agtactatgg caagaggtag tgaaaacaat
    59941 ggaaatcatt tataccaaaa ccagcctcat agccgcacag aattctcttg agctttcgag
    60001 aaagggctat gaactgctgg acaaaaaaag aaatgtgctt attatgacca tgatgagctt
    60061 tattgaaaag gctgaagaga ttcagaagaa agtagaggta gtattcggcg atgcctatga
    60121 ggcacttcag actgcaaata ctactatggg tataagcaat atagagcaaa ttgcccagtc
    60181 cataccggaa gcatcaggct ttgaagtgct ggataaaagc ataatgggtg ttgaaatacc
    60241 tgaaataagt catcaggaag agaagcttat ccactattat agcttttatc ataccaacac
    60301 ggctcttgat gtggcaatgc agaaattcca tgaggtgaag ctgctgctct atgagctttc
    60361 ggaaattgag gactcggttt acaagcttgc aatggaggta aaaaggactc agaaaaggac
    60421 aaatgcactg cagaatattc agatacccaa atatgaggaa atagtgggca agatactgga
    60481 agtactggag gaacgtgaaa gggaagactt cttcagactg aaagtagtga agaaaaaggg
    60541 tgaaaaaaaa gctgccaagt aaaggcagct ctttcttatc ccagcatttt ctctaaatct
    60601 gcttttgcat caccgcttat aagctgcagg ttgaagttct tctgcaatac ttccgctaca
    60661 tttgaggtga accattcggg agcttttggc cctatgtgga tatttttcac acccaggtgg
    60721 agaagcccca ggagtaccgc tacggccttc tgctcaaacc atgagagtac aatactcaga
    60781 ggaaggtcat taacggagca gttgaaggct tctgcaagag caatggcaat ttttgctgcg
    60841 gagcctgagt tgttgcattg ccccaggtct atatacctcg gtatgtttgt gcccgggact
    60901 gttccgaagt cgtggtcatt gaacctgaat tttccgcagg aagtggtcag cagcacgcaa
    60961 tctgacggca gtgaaagagc aagctctctg tagtagtccc tgcctttggt cggagcatca
    61021 cagcctgcaa tgacaaagaa ccttcttatt ttaccggttt ttaccgcatc gattatttcg
    61081 ggtgctattg agaggacagt ttgatgatgg aaacctgtaa taagggtctt atctgattca
    61141 atatcaactt ccggcagtga aagtgctttt tctataatgg gagagaaatc atcattaata
    61201 attttctgta ctccttccag ccctgctgtt ccgtaggaga agaatttgtc tgaatatgtt
    61261 cctctgattg gcatgagaca gttggtagtt cccagtatgg cacccgggaa ttcctcaaaa
    61321 agctttcttt gatcatacca ggctttacct gcattgcctt tcagatggct gtatttctta
    61381 agttcgggat atccatgggc aggaagcatt tcggaatgag tataaatatt gatacctttg
    61441 ccttctgtct gcttgagaag ctcctctaac gcataaagat tgtggcctgt aacaagtata
    61501 cagcggcctt ggattttgtc ctgtgatact gtaacaggaa ccggtacacc taatcttgcc
    61561 gtatgagcct tgtccagaag ctccataacc ttaactgcgg cagaacctac cttgagaacc
    61621 atgtcaatgt gctctttcag attgaagttt gaatttgtca gactcatata aagagcttcg
    61681 tgagttatgc cgtcaacctc agggtcgatg taacccattt cccttgcatg ggtagcatag
    61741 gcagctatac cttttaagcc aaatatcatt gtatcctgaa gtgctgctat atcctcattc
    61801 tttccgcata ctcccacttt tgtgcatccg ccgtgggggg tttgttcaca ttgattacag
    61861 aacatattgt ttcctcctga taatatattt tttgacttaa ttggatttta ctaaaagaat
    61921 ctgtttatgg atgtgatata aatcacacaa agggatgata ataatcataa aactttactt
    61981 ctaagcagga agcaaggcac gtggattttg ccggggtatt gctttaacag aagagattaa
    62041 ttataataaa gtagaatatc ttatgaaatc caaggattcg gcaaatagct aaggaggaaa
    62101 gaatgtgact gagtataagg ggcgctggga agcgataaga ggttctttaa tagtgacagc
    62161 tgcattaata gcattaataa catgtatata cgttttagtc cctcacggct tctgggctta
    62221 cttttttatc ggattgcttc tgctgtttat ccccactgca tatagcggcc tgatattgaa
    62281 aataacaatg gatgaaaaga agatagttgt agtaaggccc tttaccaggg tgacggttaa
    62341 gtttgaggat gtggccttat gcgcagtgaa ctgtgttggt gatggaaaat atctgattta
    62401 tgcttttgtt aaacagagat accggggagg ttatactgta aagggtataa aacccaagct
    62461 tccttttgat gaagttatta agatgtcttt aaaggaggag tgcctggatc tggatgtgaa
    62521 tttcaacagg gctaagaaaa tcccggtatc atttgtagaa aacggcgagg agcttaaaga
    62581 caggttcctg ttggaagtag gaaaacacca agtaaaaatt atgaaagaag tataaaggct
    62641 tcagatgaag tctttttttt tgaagatttt tagcaaaatg tatttattta catgacaaat
    62701 tgcaacaata tcatggcaga aaacgtcata tttcatgcta taattagttt atgaagcaat
    62761 atattttaaa ttgtgaggtg cctatgaaag agaagaagtt ttttgccgta attattgcac
    62821 tattattcgt attaatgctt gctgtcggct gctcatacaa tcaggctgca ccggcaataa
    62881 ccacaaatcc catacctgcc gacgctgaac aaccccaaga gacaactgtt accgaggtat
    62941 acgaggctcc ggatcctata gaatcagatc aagatgaaaa gcttggagtg gtatacataa
    63001 gtgccagtaa actcaaggta agagagtcgg cgggaactga tgcaaaggca ttggatgatc
    63061 ttatcaaagg cactgctgta gatatagttg aagaaaaaat ggatgattcg gggaaggcct
    63121 ggtacaggat aagctatgac ggtataacag gaagtaatga aggttggata gctgccgagt
    63181 atacagtcag ggacaggacc gagcttctgg atgaaaatct gaggaagctt gatttttcac
    63241 ctcaaaataa gacctatgaa tatccgggga atcccagagt acaagcaagg ggaatatacc
    63301 tcaccaaata ttctgcttcg aatgacaggc tggacaagct tattgaaatg acaaaaagga
    63361 caaagataaa tacatttgtt atagatgtca aggacgacaa aggacatatg ctttttccca
    63421 ccaaggctgc ggaaaagtac agtcctgaag ccaataagta tgctactgtc aaggacattg
    63481 gggcacttat gaaaaagctt aaggaaaata atatatatac agttgccagg atagtatcct
    63541 ttaaggatcc tacttatacc aaacaacacc ctgacagggc cattatatac agggacagcg
    63601 gaaagccttt tacaaacagc gacggtttga tttgggcatc tgcctacgac aaggttctgt
    63661 gggaatataa cctggaagtg tcaaaggaag cagcggaaac aggctttaat gaaatacaat
    63721 ttgactatgt aagattccct gcatcggacg gagggaaatt ggacaaatcc ctggactaca
    63781 ggaataacga aggggaatca aaaccgcagg ctatacagga ttacctgaaa tatgcaaggg
    63841 agcagctggc acctttgaat gtatatatat cggccgatgt atatggcctt gtaggctcgg
    63901 tagcggatga catgtcgctg ggacagtact gggaagcggt cagcaacgca gttgactata
    63961 tttgtccgat gatgtatcca agccattatg ccaacggcac ttatggactt ccggtacctg
    64021 atgcatatcc atacgaaact gttttcaatt ccgccaagga ttctgcagcc cggaacaaga
    64081 atatagagac acccggggcc ataaggccat ggatacagga tttttctgca ccttgggtac
    64141 ctgggcatat caagtattcg gacaagcagg taaaggaaca aataagagct cttgaggaga
    64201 atggcattgg agaatatctt ctttggaatg ccggaaacag gtattcggaa ggagcggtaa
    64261 cggaatagaa ttagagctga caagaataat atctatgcac ggttcaaatt gatgaaaaag
    64321 tatgggagaa ataatcaatg aaggaatcgg gcctgatcac aggccttttt ttttcagata
    64381 tctgaacaat aggttcaccc agacaaaagt taagatatat gttgctatgg accaaggaac
    64441 aatcctccag cccgtaaaca ctattgttct tgtatacagg cttagaattt caaaaaccag
    64501 taaaagcatt atacatatga cggtgtacac gatggcaggt ttaatccttt cgggcaggaa
    64561 taatgcatat agcatttcaa tcaccggata aagaaataca cctgatatta taatatataa
    64621 gagtgagtct gattggttga tatagtggta tagcccggca tattccccca atgctgtttc
    64681 ataaagcagg gaaaatccca tgccggcaat agcaaccatt atatgcttga aggatattga
    64741 cttcttcaga gcgcatatta gaaatatgat ccatgcagct gctatagcgc cgtaaaaaca
    64801 tgccagtgcc atttaaatat cttcctccgg ttttctgcaa acaatgctct atttattagt
    64861 atgacccaag gcagtctgat taatccaagt ctgaatatat aaacctaata atccgtataa
    64921 tatgaataat gcgacgggga catggggaca cggggacgtt tcccctgtcc cacttccagt
    64981 gggacacgcg aaacgtcccc gtgtccccgt gtccccgcgt ccccgtcgca tcattcttct
    65041 aatatatcac gctatggagg aagcagtttg gaaaaggaaa taaaaaaact gatgcaaata
    65101 tcagca
//
LOCUS       DAOQUR010000011        56712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_628_length_56712_cov_9.890375, whole genome shotgun sequence.
ACCESSION   DAOQUR010000011 DAOQUR010000000
VERSION     DAOQUR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 56712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56712
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_56712_cov_9.890375"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3078
                     /locus_tag="PKI50_03950"
     CDS             <1..3078
                     /locus_tag="PKI50_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HNT02899.1"
                     /translation="IIDGAQLSGVVDGDSVTLGDHTMGVFAQAKVGTGIGVITYMTIT
                     GDDVENYTIAQPVLTADITTKELTIINAAAQNKIYDGTDNAVIGGAQLSGVVDGDSVT
                     LGNHTVGTFAQAAIGTGIGVTTNMTITGDDADNYTIAQPVLSANITAKGLTVINAAAQ
                     DKVYDGTADADIINAELSGVVDGDDVTLGNHTVGTFAQATIGTDIGVTTNMTITGDDA
                     GNYTIAQPVLATANITAKELTVINAEAQDKVYDGTADADIINAELSGVVDGDSVTLEN
                     NDAGTFAQATVGTGIEVTTNMTITGDDAGNYTIAQPVLSADITAKELTIGGTFTAEDK
                     IYDGNTTVVIDDSDLTLTGVVDGDSVVLNAAAAFADANAADYIVVSLTDASSISGEDS
                     GNYSLSLLGAPTTTADITPPPSDSKAITDFSLEAFEPDIQAVIDEGAKTITMEVPFGT
                     DVTALVPTIEHTGADIIPASGVVQDFTNPVTYEVTAENNTTAVYTVTMSVALPSNEAC
                     LTNLVISSGILNPAFVPDIQAYTADVANSTADITVTSTAAHPGASIEVNMTAVVSGEA
                     SQAIALNVGVNTINIIVTAEDGTERIYEVTVNRASSDSDNDDDRDNDDDNDRNTSISE
                     PEPTTSSNITINVNGNSITSAASVDSETGTVTAVLNESAINNALTESQDDGQGVDTVD
                     IKIPKTTGANYYVTELPATVLTGNNTTRIEINSELGTITLPGNMLGDEGAYGIDNVGL
                     SIGTVDKSAIDEKVRTQIGDKPVIELKLKSKEGTISWNNPDAPVTVSIPYTPTEKELS
                     DPEHITVWYIDGEGNVIEVPTGRYNPVTGMVTFSTTHFSNYAVVYVTKTFDDLENAAW
                     GKKSIEVLASKGVMKGVSEKEFEPQSNITRADFLYSLIRTLGVEARFDGNFDDISKDD
                     YYYKEIGIAQRMGITKGTGNNKFNPEESITRQDMMVLTERALRMFKRIEAQGAASDLD
                     KFADKSLIADYAVNSVASVVKEGLIVGSGDGVNPLGNTTRAETAVFLYRIYNK"
     gene            complement(3144..3887)
                     /locus_tag="PKI50_03955"
     CDS             complement(3144..3887)
                     /locus_tag="PKI50_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNT02900.1"
                     /translation="MIKAILVDDELFALKELQLQLESLPNIQIIGSFTNPLESFFNIT
                     MLNPDVVFLDIDMPEVSGIYLAEQIMTTHPQVKIIFVTAFDKYAINAFELNAVDYILK
                     PFTLERIKKTISRLEEKIEKNINTDIHSLSNQYKESVKKFFIYEQDNIILLNIKDIYY
                     IEALNKFVRIRTKDRFYNSNHSLNYFENKLKNLDFFRTHRGYLVNLDKIAEIIPKINY
                     SFDIRMTDTNDLIPVSRTNIKLLKQLLEL"
     gene            complement(3865..5781)
                     /locus_tag="PKI50_03960"
     CDS             complement(3865..5781)
                     /locus_tag="PKI50_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNT02901.1"
                     /translation="MKNYKSYIIYLLIISLISLIIVIEYNSFKNNKGVPGIELKAKQG
                     ILDLTDLHFDDESLHSLDGEWSFFYNQFIMPDNYDYSAPGIITVPGLWNSYQYNKEEI
                     GASGYGTYRLKVLLPSNCNEKLALKIPDMSSSYRFFVNGKEISHNGVVGKSIEEETPQ
                     WKPLIKTLNTDSGQLDIVIHVSNFHHVKGGIWESIILGTEEAIYKHRELNLLLSFILI
                     GVLFISVFYYLVIFSVMQSNVTSLYLAMFSFMAGLRELLIREAVVFLIFPDISFKLMS
                     KLEYITVPSGPLLLALSLYSLYQTEFPRKIMSTTVTVFSLYIIVIIFTPLKIFGPLMH
                     LYLILFIIAFIYMIFVIAAAAKKKKPGAGTLLFGTLFLLLTALIDMGYVYKLHNNYDL
                     AYTFSIGLIIFILCQMHSLSLNIADVFQRAQGLAKAEMAFLQAQIVPHFLYNTLNTII
                     HLTRESPEKARSLLMELSKYLHGKFNFDLYNKNKLVTLEYELDIVKSYLAIESVRFSN
                     RLKVNYQIDEQTLNHKILPFLLQPLVENAVRHGFRKNSEDCIITISAFVQEKNLILSV
                     SDNGIGMPKNKILALTKCDYSQLGTGLTNINQRLNTAYGTDLSIVSTIDAGTTINIKI
                     PLRSDNVHDKSNIS"
     gene            6096..6686
                     /locus_tag="PKI50_03965"
     CDS             6096..6686
                     /locus_tag="PKI50_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT02902.1"
                     /translation="MARLLFQGHGSFRIISDSDAVIYLDPYAGAGYEEPADVILVTHQ
                     HGDHNQIQLPARKSTCVVIQEKDALQNGEYKSFDIKGVKIRAVPAYNKNHDRSKCVGY
                     ILTVDGIKVYASGDTSTTEEMKEYPSHHIDYALLPIDGVYNMNPVEASKCADLIGAKH
                     TIPIHMAPGMLFDRHKAEQFKGKNRLIVSAGDEIEL"
     gene            6705..9185
                     /locus_tag="PKI50_03970"
     CDS             6705..9185
                     /locus_tag="PKI50_03970"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT02903.1"
                     /translation="MSMIHRVLQRKHINEQLKSVFAYPLTVAVAAMGYGKTTAVREFL
                     DRANAKYVWLSVESDYRSAGHIWDSLTRQLAKLRPDLGNQLNALGFPADASQRDKIIS
                     IIEEHTYLNDIVLVLDDYHYARSQELDKLLERIVRTNIEGFHIIIISRTKPEINIEEL
                     SLKGYCFMIASELFELSRDEIREFFKLYGCRIPDNSVRTVHEISEGWITAVYLILHRY
                     SATGRLETGRNIESLIESAIMSRYTEEESQILKSICILDSFTLQQAVYVTGNAAAAGI
                     IERMSAGNSFLRYDEQLDVYKMHNIFSEYLKKQIEKQGSSIEAEALYKRSGQWYIRSG
                     DILSGLSFLLKAKEYDLMLTEFERHGATQELDKDPQLIVEVFSQIPDEVKYRHPMSYL
                     TYAYNYISSVDMEGGAELLAQIEEHYRYDESIPDELRRRIAGEIELAGSFLYFNDLEK
                     MNEKYLNAYSLLDGKSSIANKDMIFTFGTPHTLYLYYREKGRLLWIVEYLDNYFHYFC
                     ELSKGCGTGFEELARAEYCLETANFNQAELYAFKSIYKAKPMEQTSLIICASLTLARA
                     YAAQGKFDKARELLDNLAAETAQYNNPVYNSTLDLCKGYLGGITCRLQDFAPWLESGD
                     MEQSDILFLGMGFNYIVHAQAVLLQKNYIKLEVLCEGMLQIFSMFNNLLGFLHTHILD
                     AAAKYNLYGIERAKAAMLQAADIGRADDIILPFAEYGAHILDILKALDNDEKGDEYIS
                     RLISAAEQYSTGLERFKEEKISVPSLTKREREVLKLVAEGKTNREIGAELFIAEVTVS
                     KNITSIYQKLGVTGRPSAVKKAIELNIL"
     gene            9293..9526
                     /locus_tag="PKI50_03975"
     CDS             9293..9526
                     /locus_tag="PKI50_03975"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HNT02904.1"
                     /translation="MPKIIRVEPKDDHTLLIELNNKHMIVYDMKPRLETVRFCGLADL
                     NRFKDVRIEDENTLVWDSLCQITIDEVLSMIER"
     gene            9560..14503
                     /locus_tag="PKI50_03980"
     CDS             9560..14503
                     /locus_tag="PKI50_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HNT02905.1"
                     /translation="MKNNNTIQLLVKTIAITAMFLLAAFLLIPDTVLAEGGFARGDGS
                     PEQPFEIATPEQLATVAEEVYCSSHFKLIDHIDLSDYLSDGGGGWNEGAGWKPIGTYD
                     MFDPTKAFTGSFDGDGYTISSLFINRSDTEGVGLFGYTVGATIKDVTLSDADITGDAC
                     VGSLVGVNENCTISGCSAENAEVTGFTTVGGLVGSNDSGNIANSLATAEVSGVFSVGG
                     LVGDNYGGSIESSYATGTVSGIDYDDPESQGHFGGLAGRNYIYGTIENSYSTGNVAGI
                     HLVGGLVGSNTDAGVIENSYSTGDAAGTAYVGGLVGLNSLDEESYDNPFITDCYAAGS
                     VEGEQYVGGLVGQNCEGDIVHSFSIGEVSGTNKVGGLVGENEDGTVTDSFYDSNTSGQ
                     SDEGKGERKSTAEMNAYTTFFNASWDFIGEMDNGNEEIWGIDESADNPANDGYPFLTW
                     QGYGHKVWPAVSTMEAEDITDITAVGQGGIIIIGYPNPTQHGVVWSSHEDPTLEDNKT
                     EKGAVLGAGSFTSEITGLEPETTYYVRAYATNDLGTVYGEQISFTTEEGTFAGGNGLP
                     ENPYQIQTADQLAAVSEYMDSYFVLVDNIDLSAYGDGYDGGKGWLPIGNYDNSFTGSF
                     DGNGHTITGLYINRPDTYYVGIFGCTGADAEISNLRLLNVDVSGYGYVGALVGENHGT
                     ITGSCAAGSVEGSDYVGGLVGLNWEATGNIIDSYSAVNVVGAESVGGLAGGNEGTITG
                     SHATGDVVGEDYIGGLTGGSGTSDYRCSITDSYATGNVTGNGIVGYVGGLTGQNFNAD
                     IENSYAVGTVTGSNNVGGLVGINEADGTVTSSYYDTETTGQTDGGKGVPKTTAEMKQQ
                     GSFVGWDFTDTWNLAGGTYPYLEWQTDNFDFAPPVFTVGYPKAEDITYERMDILVQVD
                     EVAAAYYVVLANGVDAPDAAQVKAGMDSENTVLAENLKGSIAGISAGTDGRAVITGLA
                     PGTEYDIYVVAEDNAGNMQPDGSVALLEACTGLMPTGNMSFALTTPVPEADGNSIAVD
                     QSSPKIVDVDTVNGIGSVEITGTKTAAQSVTVGGVDADKVTAAGEATEPVYTIDTSAI
                     AAEGGTYEFTLTVSEAGKADIVYNVTVTVAVPDNTPPVPGNGGTITVSNITHNSLELS
                     WAAASDDVTAQQNLQYKVVRSESNNIDTAINAESNGTIVKDWTAGLTGTEASGLSAET
                     IYYFNVIVQDEAGNMAVYTMKSQAAGSVPEREDEDDRDEDDRSERNVSGNSPVYNAVI
                     KGSNTSGTTITVNVNTRRGSAVVNLGEQFEDVSNGEEVPLITIPSIPGVNAYTVGIPA
                     DSLSGSQSKDILTFSTPEGSITLPGNMLAGIAQTGSKGAGITVGEGDKSLLPDDVKAA
                     LGDKPLVQLSLTLDGNQAEWNNPDAPVTVSIPYKPTAAELSDPEHITVWYIDGSGNVV
                     EVPSGRYNPETGMVTFSTTHFSHYAVAYVTKSFNDLGSAAWAKKPIEALASKGILKGI
                     SETEYAPQTNITRADFLYSLVRALGIYAKVEGNFDDISRDAYYYREIGTAKKLGITGG
                     TGNNKFSPDESITRQDMMVLTERALRMLKKLEVQSSASDLDRFADKTLIAAYATDSIA
                     SVVREELIVGSGDRINPLGSTTRAEAAVFLYRIYNRF"
     gene            14869..15432
                     /locus_tag="PKI50_03985"
     CDS             14869..15432
                     /locus_tag="PKI50_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT02906.1"
                     /translation="MGPKIKFTREQIIDAAFEIARAEGIDNVTMRKIAHRMGSSVAPI
                     YVNFKSINELIEALIEKIADIGQQLIAEENTGSPFADIGRASIRFAMEYSVLFRDLVM
                     KNSRYLKGYDEKVMPALIEEMKKDSDLEGFSEDKLKEILLKMRIFQLGLSVMAASGIL
                     SKDYDMKDLTDLLSSTADDVILSARLK"
     gene            15636..16142
                     /locus_tag="PKI50_03990"
     CDS             15636..16142
                     /locus_tag="PKI50_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT02907.1"
                     /translation="MDKIVIIGGGIAGLSAGIFAQKNGFESVILEKHNVLGGECTGWN
                     RRGYHIDGCIHWLVGTKKGTPIHDLWTAAGALDGVEIYHPESFLAFEYDGIRVHLYRD
                     LDRLKSSWLELSPQDEAAINNFCCTIKQLQSFEIPVGKPEDMMEFMEKIKLMISLFIP
                     GRRYRSPL"
     gene            16153..16566
                     /locus_tag="PKI50_03995"
     CDS             16153..16566
                     /locus_tag="PKI50_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02908.1"
                     /translation="MNIEGNRVRRVICSSGKAFEAGYYIAACDAHVLYEKLLKGKYPD
                     PEFQKRFNNPEDYPLASEIRIAIGYEGRTDEIPRTLRFPIDSFKVNGRPINYLTITHY
                     GYEPDFAPEGHVLITCSINQFHADYDAWNALAAHH"
     gene            16604..16729
                     /locus_tag="PKI50_04000"
     CDS             16604..16729
                     /locus_tag="PKI50_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02909.1"
                     /translation="MENIFLSGQWLQPPGGLLVALITGKDTIMRLCKMKNKLFVY"
     gene            17002..17931
                     /gene="rarD"
                     /locus_tag="PKI50_04005"
     CDS             17002..17931
                     /gene="rarD"
                     /locus_tag="PKI50_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNT02910.1"
                     /translation="MDGKKEGLQVKNDVSGVVYGVIAYTVWGLLPIYWKLLGEIPADE
                     ILAHRIFWAFLFVGGLFLSKNDIGVLKEAIKERKNIRNILICSFLITINWGTYIWAVN
                     SGRILESSMGYYINPLMVVLLGMTVLKEKLNVLQYISIVFAAAGVIIITVQFGNIPWV
                     SLLLALSFALYGLFKKLLKVESLTGMVLETAAIMPLALGYILFKLFSGQSALYSVPLS
                     TAAILMLSGIATALPLLWFAMGAARLKLSTLGFLQYISPTLSLLLGVFLYGEKFTVTH
                     LLSFSFIWTGLIIYSFSNFEAKSKFGNSKQADV"
     gene            18083..18529
                     /locus_tag="PKI50_04010"
     CDS             18083..18529
                     /locus_tag="PKI50_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNT02911.1"
                     /translation="MEVAEFKNIVFDYTRKINESLHCAFGPAIGNRGLTMMQTRILME
                     LHRCESHTIGSLADSICAAGANISAMCKKLEEQGFIERIRNRKDERVVRVVLTRLGKE
                     TVLEIDELFNERISKCLKYESEEAFEDIIRGMQKLNELLTRIIKNE"
     gene            18565..20382
                     /gene="ftsH"
                     /locus_tag="PKI50_04015"
     CDS             18565..20382
                     /gene="ftsH"
                     /locus_tag="PKI50_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNT02912.1"
                     /translation="MKEQNNPRKPIIYFYIVTLVIVMLLNAYIFPAVVKQQVTQVDYG
                     TFLTRIDEGKVKSVEIQDNQIAFTAIDEAGKENIYVTGKVNDPELVDRLLAADIEDFS
                     QVIPRENSPLMNFIISWVLPLIFFIGMGQLLAGSMQKRMGSNAMTFGKSNAKIYVEAQ
                     TGKTFDDVAGQDEAKEALSEIVDFLHNPAKYKEIGAIMPKGALLVGPPGTGKTLLAKA
                     IAGESKVPFFSISGSEFVEMFVGMGAARVRDLFRQAQEKAPCIVFIDEIDTIGKKRDS
                     GGIASNDEREQTLNQLLAEMDGFSSEKGVVILAATNRPDSLDKALLRPGRFDRRVPVE
                     LPDLAGREAILKVHAKKIKLGNDINFNEIARATPGASGAELANIINEAALRAVKCGRK
                     YVKQIDMEEAVEVVIAGYQKKGAVISPKEKEIIAYHEIGHAIVAAKQTESAPVHKITI
                     IPRTSGALGYTMQVAENENVLMTKEEALNKIATYTGGRAAEELVFGTYTSGAANDIEQ
                     ATKIARAMVTRLGMSKAFDMMGLETISNVYLGGDPSMTCSSETASKIDEEVLAIIKSA
                     HEKAINILKENMEKLHLLSSYLIEKETITGEEFMRILES"
     gene            20602..21522
                     /locus_tag="PKI50_04020"
     CDS             20602..21522
                     /locus_tag="PKI50_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT02913.1"
                     /translation="MNALKRISQVFEASEQITFDDSSKIILMSDCHRGDGSWADDFSR
                     NQNLYFTALTHYYKNNFTYIEIGDGDELWENNNFFNIKQVHCNVFWLLSRFYSEGRLH
                     MIYGNHDMVKKYNRFVRSNMHRYLDEPARRHMPLFINIKFHEGLILKYRPTGGKIFLI
                     HGHQVDCLNDRLWKLARFLVRYLWRPLNNLGVNDPTRTAKNYKKKETAEKKLTEWVIK
                     EGHVLIAGHTHRPMFPEVNEPPYFNDGSCVHPRCITGIEIAEGYILLVKWSVKTKDDG
                     TLFIGREVLEGPRKLVDFFACKDRTKKLLL"
     gene            21587..22456
                     /locus_tag="PKI50_04025"
     CDS             21587..22456
                     /locus_tag="PKI50_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT02914.1"
                     /translation="MKRLAYVFVISAAVLWGTIGIYSNQLSQYGANVLQIVSIRAIFA
                     APAIFVFILLKDIKLLNIDIHDWKYFFGTGILSFVFFNWCYFTAIDKTTLSTAAILLY
                     TAPAIVMVFSIILFKEKLTRRKVAALILTFIGCAFVTGVVKGSQDISPLGILAGLGSG
                     FGYALYSVFGRYALKKYDPITVTLYTFVFASIGLIPISDIKGLFGLLSSTVAFYNAVL
                     LGLAATVMPFLLYTKGLSYLETSKASIIAAIEPVVATIIGIILFGEPITVFKIMGVFL
                     VVFAVSILEQKKS"
     gene            complement(22777..23790)
                     /locus_tag="PKI50_04030"
     CDS             complement(22777..23790)
                     /locus_tag="PKI50_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNT02915.1"
                     /translation="MNYDEVLKNAKANLNGTCRVCPVCNGKACSGEVPGMGGKGTSEA
                     FTANVDALKKYKLNMRVLHNCKNPDTSIELFGKKMSIPVFAAPVAGTNFNMGGKFQEQ
                     EYISWIIKGCIQGGIYPMFGDAAIDSLLVTNLEELKKSGGEGIAIIKPWTNDKVIEKI
                     RMAEKAGAYAVGIDIDAAGLITLSLLGKPVGPKSVEDIKEIAESTKLPLILKGIMTPD
                     EAELAVESGAAAIVVSNHGGRVLDQTPGTADVLPEIAKKVKGKITILADGGVRNGVDV
                     LKMLALGADAVLIGRPFVPASFGGQAEGVKAYIDSIAADLKSAMILTGCNSIEDVSSR
                     ILF"
     gene            complement(23894..25531)
                     /locus_tag="PKI50_04035"
     CDS             complement(23894..25531)
                     /locus_tag="PKI50_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HNT02916.1"
                     /translation="MNSLVLLFIGIAAFLIAYTIYGKWLANQWGIDPTKKTPSCTLRD
                     DIDYCPANAKVLLGHHFSSIAGAGPITGPIAAAVFGWLPVYLWIVVGSIFVGGVHDWG
                     SLFASVRHDGKSIGEIIRANIGENGKKLFNIFAYLTLILVVAAFTDICAGTFAFDPAK
                     PEAITGARAGTASILFIFLAMGFGYFVYRKGASLSVSTVAGVALLFFCIWIGYQFPVL
                     KFSKLTWDVILIIYIFSASTLPVWLLLQPRDYLCSFLLYAMLAGAVIGVLLVRPAMAI
                     PASVGFTVGEGAKAQYLFPMLFVTVACGAVSGFHSLVASGTSSKQVDNEKDTQLIGYG
                     GMLIEGVVAIIALIAVSYVAGATGTPAQIFAAGVADFMSSFGIPVPVGTVFVTLAFSA
                     FALTSLDTATRLGRYMFQEMTTKADGTKSPLNNMYTATAVTVGLSALLLSYGYTKIWP
                     IFGSANQLLAGLALLAVSAWLAKSGKKNTMTIIPMVFMFCVTLTALVLVARTNFAGGN
                     VIIGSLAAILFVLAIVLIITTIKTFSGDKSKNQNVDA"
     gene            complement(25617..26123)
                     /locus_tag="PKI50_04040"
     CDS             complement(25617..26123)
                     /locus_tag="PKI50_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02917.1"
                     /translation="MKYDEYIKLLTSKLEKNFAIERDIVIFDNKIDILAKFSNISVRT
                     FITQHDIIDQYNNHEHIYIKRCDDVNEKDIISFGQFLKRISDECIKPDKDHMSTYVTG
                     VLIGNHINENAIKAVEKYNYKKAFCFYLKGWCDVRLIYVGLDNSEIITNKDGKKVKKL
                     YEFASLNK"
     gene            complement(26326..27027)
                     /locus_tag="PKI50_04045"
     CDS             complement(26326..27027)
                     /locus_tag="PKI50_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNT02918.1"
                     /translation="MNCIIVDDEYPSIQELSYFITNFSSITISETFDDSIKALEYIQR
                     HSVNVIFLDINMPKLDGLTFSKVINTLPVKPVLVFITAYREHALEAFEVSAFDYILKP
                     YSQTRIADTLQRLENSTAVRLHNDKITLWKNEKLYVLDADDIYYCEAHKHEVYVYTKD
                     KRFVVASSISDFHKKLRQDCFFRCHRSYIVNIDKITEIIPWFNNTYMLKLKGLDAEIP
                     VSRQNILLFKKLMGI"
     gene            complement(27067..28737)
                     /locus_tag="PKI50_04050"
     CDS             complement(27067..28737)
                     /locus_tag="PKI50_04050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNT02919.1"
                     /translation="MIDLIKNLINSLIYVIIVAFIVSNLSVFKKIIQKDAFKKSDLLI
                     LSVIFAVFGIISTYTGTDVLGAIANTRIIGVMTGGILCGPFVGIVSGIIAGLHRFLYD
                     IGGITSVPCAISTILAGFLSALIYKKGIRYQKWLYGLAGGIIIESIEMLLILLMSKPF
                     SSALAIVKSIYVPMSFTNAIGICILILLIQNSFKEKEQMAAMQAQLALEIANKTLPYF
                     REINGDSLKKVCTIIRESTGAAAVSITDKTTILAHVGLGSDHHISGDPIQTFATKQVI
                     MNGQMRIMNSPEEIECSSEDCQLKSGLIVPLKEKDEIIGTLKLYFAKRNGISFTHEKL
                     AIGLSQLISTQIEISKVGKLEELAAQAEIKALQAQINPHFLFNALNTIIALLRINPNS
                     ARELIVNLSTYLRYNIEETSIFVDISKELQQVRSYIEVEKARFGDKLNVIYDIDESIH
                     VKIPSLVIQPIVENSIKHGILKGSGTGNIVISVKKHNPDEIRVSVEDDGIGIPQAFIE
                     NLHAGKVKENKIGISNVDNRLRHIYGTGLNIERLDVGTRVSFMLNMSA"
     gene            complement(28881..29336)
                     /locus_tag="PKI50_04055"
     CDS             complement(28881..29336)
                     /locus_tag="PKI50_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT02920.1"
                     /translation="MTLEEYKKIISDAIKNEEEAYSFYRDVAKKTKDKNISEIFENLA
                     KDELSHKVILQDYFKDTGKKLEFDSTADYKVAESVDKPKLSIDMKPADAIALAMKREE
                     EAMDMYKGFAAISTDKEQKETFLGLAAMEQGHKTKLEDLYVGAAFPEVW"
     gene            complement(29543..30112)
                     /locus_tag="PKI50_04060"
     CDS             complement(29543..30112)
                     /locus_tag="PKI50_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02921.1"
                     /translation="MKGKVIVLVFAVIIALFAGCAPEPAAPETPPQTTPPEATPQEAT
                     PDVVTTASIVADADAFKKAISGNGTWIICTTKDLSINEDLILEGEFHDKNDPAKDLKR
                     KIALYAQDADKNVTARYTLTAPKLTVKSPNARIQGGTFKGDVYVSAKNFELVDGTIDG
                     NVYFTTDDAKSTFKIDDKSKVTGKQELKK"
     gene            30402..32387
                     /locus_tag="PKI50_04065"
     CDS             30402..32387
                     /locus_tag="PKI50_04065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT02922.1"
                     /translation="MQEAAGAFKLYIAKNNAVMIQAADNQKAVFYFFIILLIAAAVAI
                     IHYYAARNRRGTVNSKKEGVISYRTIIQLMQEGFALFEIIYDQGGKPIDYYFIDSNEA
                     AEVISGIERKTSINKSVFDILPDSAAELAKKLNEVTFAGEPYKDEIYVKEWGKYLSVN
                     MYKAEKKLLAVMFSDITQKKLADEAMQKQKLSYEALFRNSSDAIIRFDQNHNIIDINE
                     KFTELFGYTLDEIYGRELDSVLAPKEKFDEAKVLTKNVLNGNITAMEESVRLGSDGKT
                     REVSIKGVAIVLNDKIIGGYGIYTGISRRKKAERELMYMNYHDLLTGLYNRRFFEEEL
                     KRLDTERNLPISIIMADVNGLKLFNDAFGHAEGDKLLVKAAEIISKELREDEIVARLG
                     GDELVILLPGTDSEEARSIVKRIRASISNIRLKSIELSISFGWDTKNSADQDMNDILK
                     NAEDYMYRNKLFESPNIREKRIKAIINSFYENNSEEEQHSKRVSSLCEATGAAMGMAG
                     AALKELRTLGLLHDIGKIAIDNSILNKPDRLTKEEWNEIKRHPEIGYRILSSVNDMAE
                     LAVFILAHHERWDGKGYPKGIKGTDIPLQARITAVADAYDAMTSKSAYRNPLSESIAV
                     DELEKNAGTQFDPEIVQIFIDKVINKKVKTSVDIDVT"
     gene            32428..33192
                     /locus_tag="PKI50_04070"
     CDS             32428..33192
                     /locus_tag="PKI50_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT02923.1"
                     /translation="MEYTVQKLGKLAGVSTRTLRYYDEIGILKPARINASGYRIYGQK
                     EVDRLQQILFYRELGVGLDSIREIVTAPSFDGAKALREHRGKLLEKREQLDMLIANVE
                     KTIALTEGRISMTDKEKFEGFKQKLLDDNEKKYGKEVREKYGESAVEKSYDKVKNMTR
                     EEYDELTKLSEEITKILGEAFKTGDPAGDLAQRAADLHKQWLTFYWDSYSKEAHAGLA
                     QMYVDDERFRAYYDKKQPGTAEFLRDAILIYTGMKK"
     gene            33383..36262
                     /locus_tag="PKI50_04075"
     CDS             33383..36262
                     /locus_tag="PKI50_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT02924.1"
                     /translation="MKCISKFIIFLFALLLTLSAFPVSAASEAPEKEELRGVWVATVA
                     NIDYPSKSTADSAILKSEALKILDDAEAMGLNAIFLQVRPASDALYKSKLFPWSKYLT
                     GRQGLAPDNGFDPLEFWAAEAHKRGMELHAWINPYRITKKTPKEPKHDLASLDPSNPA
                     RLKPEWVVKHTDGNLYYNPGIPEVRKLVIDGVLEIIENYGIDGIHLDDYFYPGKDFSD
                     KAAYAKYGTAYGNIDDWRRENVNKLISELSKAVKAADEAVSFGVSPFGIWANKSANTL
                     GSDTRGMQTYYDQYADTRKWVKEGLLDYIAPQLYWYIGYEIADYSRLLSWWADVTAGT
                     GVDLYIGQAAYRTGDTDPKSPWYGIAEMEKQLKLNSENKEIKGSIFFSNRSLCNNPAL
                     CAALQSLYKRKSSIPISVSRPAEDIQTTLEKYYLNGSSDPEKPLYLNGVPVENRSARG
                     YFGVLVPLTEGANSFTLSQEASEVTRVIYRKTADSEPAKMSGAEIISASVFPQSQEYR
                     NPGEKITLSCEAPYGSTVTVQIGGKRYLMKSSGAVTGTSGLYADKFTCEYTIPAYKGT
                     PRNIDLGKPVYTMNYKGTAKSCTAPAKIGVIMKGSPFYAKVEKEVIYTYNEASSSDGA
                     AYELYSGMVDSVTGMSGGYVRLSLGQWVKDSDVAVYTSKAQSRPIIKDTEYISGDGWD
                     SLKLDISAPPAAIADFDGALLKINVSADSQAASPELPDDSPFSSVAVAKKVNNTEYTM
                     ALKKNRSISGYYMQKTKTGLVLNIKRPVKSNNSDEPLSGLTIMLDPGHGGSDTGAIGP
                     LGTEYPEKTINLNIAFKLQSELEQLGARVFLTRAADVNVSLEERLAASRNAKPDMFLS
                     LHSNSMAEDVDISKVDGFAVFYREKLALPLAETIFQNTLTHLNRTNKGLHNKNFYVLR
                     GTWTPSILVESGFVPNPYEFEWLIDENEQAGLAQSIAKSVVEYFK"
     gene            36332..37444
                     /locus_tag="PKI50_04080"
     CDS             36332..37444
                     /locus_tag="PKI50_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02925.1"
                     /translation="MSELRKMKAIVLLSIAIMTAIVILPASAAVGQQPPFSEVKEKLS
                     GISKEEKEILQKLFILAQEIEIAEAEAEELSRETEAISKEIKGLEEDIAKEELAYEKK
                     RKSLEQVLKSYQRMGAGSFLEIILDSDSLSTFLHRLNILRDLTKNTGTLLEQLESSGR
                     KLAEEKSKLSEKLALVKDKESMSRTALADKNRLKAEKEAYLASLKGEKEHYQEYLSNL
                     ERVWNELKPLLSETAKEFSRMIEEEGLPYDALKISFSLFEVKGAIDDGTINKIISENA
                     NLEEMIFAFHQEAAEIRIPDKNLILTGTFAIQEGHTLKFQAREGSFFGLPLEKGVLEE
                     LFGEEGMMLNLEPLLAGNDIHAVRIKEGYIELIHRF"
     gene            37472..38146
                     /locus_tag="PKI50_04085"
     CDS             37472..38146
                     /locus_tag="PKI50_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT02926.1"
                     /translation="MIEAKGVCLEYPDGTKAIKNIELSVKPGELVYITGPSGSGKTSL
                     LKLFMGEEYPTAGSLRVLGQHISRGQASRIRGLRRMIGPVFQDFRLIKGRTALENVML
                     GMRFLDIPSGCIRDSAGNALKKVGLEQKMLSLVEHLSWGECQRVAIARAVSREPVLII
                     ADEPTGNLDKDNALNILELLKSFRNDNKAIIITTHATHLIEDERYAVHIRMDNGNMLK
                     ERSGEA"
     gene            38147..39031
                     /locus_tag="PKI50_04090"
     CDS             38147..39031
                     /locus_tag="PKI50_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNT02927.1"
                     /translation="MKNILYNTGYFIKETKTIMRLNLLSSILSFLSIGLIFFILAMMI
                     SGWWVSSHVVLAIQEEAEISIYFSEDMDAAGAERLAEEIENIEGVYEARLVSEEEAYS
                     RMEKILGKEARVLEYLDDNPFSPFIEAKIQIEDIGLIPGKLDLIEGIEHVRDNREVLE
                     RILGISQTIKILGYLIIAAVGISTTVIISHIIRMGIYDCREQINTLRLMGAPEAFIGF
                     PFMLEGLLLTLGGGALSSALAAYALKYLYAQAAGPIAFIPLPPREALVSDLTILIMLL
                     STALGTAGSFFGLSSAKN"
     gene            39113..40300
                     /locus_tag="PKI50_04095"
     CDS             39113..40300
                     /locus_tag="PKI50_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HNT02928.1"
                     /translation="MSFKMTGKVTWVGKIDWELRRFHGDEYSTHRGSSYNSYLIKDKK
                     NVLIDTAWAPFAKEYVETLKKEIDLDKIDYIIANHSEIDHSGALPLLLAEIPNTPVYC
                     TANGAKILKGHYHKDWNFVTVKTGDTLDIGESKLTFVEARMLHWPDSMFVYMSGENIL
                     FSNDAFGQHYASELMYNDMVDNAELYQEAIKYYANILTPFSTFVTKKIEEVLSFNLPV
                     NIIAPSHGIIWRDNPAQIIQKYMDWAKDYQENQIVIVYDTMWNGTRRMAEAIAEGIRS
                     VDKEVAVKLFNAAHSDKNDVITEIFKSKAVLFGSSTVNKSIMHAVGGMLDMVKGLGFK
                     NKKAAAFGSYGWSGESVGILTERLKDAKFEIVSDGIKELWNPDQDAMNRCVQFGADFA
                     GKL"
     gene            40387..41334
                     /locus_tag="PKI50_04100"
     CDS             40387..41334
                     /locus_tag="PKI50_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02929.1"
                     /translation="MFNKKAFFRSASLVMSIVVLLGAGLLTGCTAPAEDNTGGKAEDN
                     NPPAVEESIEDRIIPEFMALVGGNPKPDAIIEFMDKSIADVSGENASKMLDELEKSLK
                     SNLPELEEKYYSTAVQEAMFNAYKSEFDLSKLDSIQDAEVKSLIDETKAMGYKVETAE
                     GMFFPIINYEFLKKFSSYAEDDMKDYIDIMAAESNKVPAKDGALVIGWDEVIERALAQ
                     ENFMAKHSSSAKVGSIKELQKKYIMFILYGLNNTPLFSYDTKMMNPEAKDAYTKVLKE
                     KGDSELLQLLGGYLEILEKSDYKLTEEADKYRKDAEGQL"
     gene            41544..42515
                     /locus_tag="PKI50_04105"
     CDS             41544..42515
                     /locus_tag="PKI50_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT02930.1"
                     /translation="MLLLIIVFISSFCFGRYNVNLHELLRILANRVIPLEHTWTAEME
                     TVVINIRLPRIVLACLVGCCLSVAGASYQGVFQNPMASPDFLGATSGAAFGAALAILL
                     GSSSRMITILAFFFSLVSVFLVYFIGRYAPGKGVINMILAGIMISSLFTAGTSFIKLV
                     ADPNNQLPAITYWLMGSLSGAKNNEVVFALIPMAIGMIPLFVMRWRINILTLSDEESN
                     AIGINANMLRLIVIISSTLITAASVSVSGTIGWVGLVIPHLCRRMIGNNYTYLLPASM
                     AFGATFMLLVDNISRNLLATEIPIGILTAFIGAPFFIRLIFRREGTL"
     gene            42512..43297
                     /locus_tag="PKI50_04110"
     CDS             42512..43297
                     /locus_tag="PKI50_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02931.1"
                     /translation="MIIEVEKLCFGYSSHKVLKDISFNAGKGKLISIIGPNGVGKSTL
                     FRCVLGLISEYSGSIRIEGNDIKKLSVKSRAHLMAYIPQTYYPSFNYSVIDMVLMGTA
                     HQLSAYSMPGKRETEQAWKALEQLNITGLAQRGFCKLSGGEQQLVLIARALAQQAGII
                     LMDEPTSNLDFGNQLRVLQQIKNLTKEGYTVLLSMHNPQHALTYSDYILALFDGSIIG
                     DGTPHEVINEDLLSRLYNVEVQIAEVGGDMLIVPSGKGRVDCV"
     gene            43290..44045
                     /locus_tag="PKI50_04115"
     CDS             43290..44045
                     /locus_tag="PKI50_04115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT02932.1"
                     /translation="MFKWDLRMIQFMKDAIEENDIYFKSLAGKVSEYLPADAHVCDAG
                     CGLGYLSTELSKYCRNVTAVDIASLALVELRNKLNHINCNNIKIVEGDIRVNPPGIPY
                     DAMVFCLFSSLEDALKISKEQCRGTTVMIKRNWNNHLFSISQKALERHTLQESLEHLK
                     MLRIPYKSENFILEMGQPFRSMADAVEFFQMYSNDDYPHEIKAEDIADRLVYRESDRF
                     PYYLPALRQLGIIVVKTSDIPDNIEIIWRRNEE"
     gene            44042..45139
                     /locus_tag="PKI50_04120"
     CDS             44042..45139
                     /locus_tag="PKI50_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT02933.1"
                     /translation="MKRIIAITIAIVMILTMLAGCKAPEPASAPAEAEAIAQSGSDNA
                     TVVFTDSVGREVEVPANIEKVAISGPLAQIVVFALAPDKLVGITAPWDKSAEEFIDTQ
                     YYDLPILGQLYGGKGELNLETLLASGAQVIIDVGEPKKTIVEDMDELQKQTGIPFVHI
                     TAKTETMDEAYQILGKLLNMEVQAQVLSEYCKQTYKRTREIAGSVNKVNLLYCLGDKG
                     LNVIAKDSYHSEVIDLLSNNIAAMDNPSSKGTGNEVDMEQILNWNPEVIIFAPNSIYG
                     TAGTDPVWQKINAIKENRYYEVPNSPYNWLGFPPSVQRYLGMMWMSQLLYPETAKYDL
                     YKEVQNYFKIFYHCDISMEQYEKLVVNSIGK"
     gene            45152..45688
                     /locus_tag="PKI50_04125"
     CDS             45152..45688
                     /locus_tag="PKI50_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNT02934.1"
                     /translation="MSRPQHILICGERGVGKSTLVNKLLLQSRRPVFGFITKKMPPNQ
                     NGECDVYIHPANSANLQLTHENLVGICNNGKIVTINTEVFNTVGVKLLQSPPRGLLLM
                     DELGFMESKASAFCSGVLAVLDGDIPVLAAVKNKETPFLKAVREHKNVELHYITTQNR
                     DMLYQELIEKIMEYNQWK"
     regulatory      45752..45860
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45981..46472
                     /gene="thiW"
                     /locus_tag="PKI50_04130"
     CDS             45981..46472
                     /gene="thiW"
                     /locus_tag="PKI50_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HNT02935.1"
                     /translation="MQTQKLTFSALLVAIGTISANLVYIPVGAAKCFPVQHTINVLSA
                     VLLGPWYGVANAFLISLLRNILGTGSLLAFPGSMIGAFIAGIMYLKFGRKLFAAAGEI
                     FGTGILGGLLAFPIAKFLMGNDAAAFYFVVPFLISTIGGSIIGYVILGVMEKSHVLKK
                     TGI"
     gene            46487..47293
                     /gene="thiD"
                     /locus_tag="PKI50_04135"
     CDS             46487..47293
                     /gene="thiD"
                     /locus_tag="PKI50_04135"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNT02936.1"
                     /translation="MKNVLTIAGSDSCGGAGIQADLKTFSAHGVYGMSVITAITAQNT
                     QGVLAVQDISVELIEKQIEAIFEDIRVDGLKIGMVSQIDTINAIGEELRYYKPSNIVL
                     DPVMVSKSGYHLLQQSAKEALIKSLVPLATIVTPNIPEAEVLTGMRIGSIEEMEKAAR
                     VIFEMGPCNVLLKGGHLDEDATDILFDGSKISCLKGKRINTKNTHGTGCTLSAAIASN
                     LAKGYSVAEAVALAKEYITIAIENSLSIGKGVGPTNHFYTLYKKAGMLDE"
     gene            47286..48512
                     /gene="cytX"
                     /locus_tag="PKI50_04140"
     CDS             47286..48512
                     /gene="cytX"
                     /locus_tag="PKI50_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HNT02937.1"
                     /translation="MNKSNNNLTLLSFAALWFGAAVSIAEILTGGLLAPLGFKNGIIA
                     IIIGHIIGTLILVAGGIIGTETGLPSIASTGISFGKYGAYVFSVLNVLQLLGWTAVMI
                     ISGARSVNEIAKVLWSFDNIYLWSVVIGGLICLWIFFGNVGVKKLNLIAVVLLFILTV
                     MLSAVVFKNSELFSSSGDGTLSFGSAIELNVIMPLSWLPLIADYTRFAKRKGDAVTGS
                     FLGYFTGSTWMYTIGLGAAIASGNSDPGAMMLSANLGFTALGIVILSTVTTTFLDANS
                     AGVTFLNLFPKLNERKTALGMGAAGTLIAVIVPIEQYQNFLYAIGSVFAPLFAILLTE
                     YFVLKKSRKLQEGLLVNWGASAVWVAGVIMYYQFIKLDFVIGATVPVMIITGLLYCLS
                     WRWTESWILLEKHRNC"
     gene            48479..49291
                     /gene="thiM"
                     /locus_tag="PKI50_04145"
     CDS             48479..49291
                     /gene="thiM"
                     /locus_tag="PKI50_04145"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HNT02938.1"
                     /translation="MDIIGEASELLIRIKEYRPLIHHITNYVTMNDCANAVLAIGGSP
                     IMAKDPSEVEEVAAMSRALVLNMGTPDNNNFRSMLLAGKKAKEMRIPVIFDPVGAGAT
                     SFRREICCKLLSDIKPDVIRGNISEIMYLAGKDRYSCGVDSNWDIDNAVGIVQGLAKD
                     LNCVVAATGRKDLVSDGSTAYIINNGHEMLSKITGAGCMTSSIVGTFCSMPEDYLTGA
                     VGGVMVMGISGQLANACLMPNEGIGMFKVRLFDSMYNLNRDIFMREGVVYAG"
     gene            49281..49904
                     /gene="thiE"
                     /locus_tag="PKI50_04150"
     CDS             49281..49904
                     /gene="thiE"
                     /locus_tag="PKI50_04150"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNT02939.1"
                     /translation="MPDKGLVNYSLYLITDRKVLGGKELLDCIEDAIKGGVTLVQLRE
                     KNINTFDYYNLALKVKELVRKYGIPLIINDRVDIALAVDSEGVHLGPEDLPIAAARKL
                     MGRDKIIGASANCIEEALDFQRQGADYLGIGALFPTKTKGNTEHVTLTQLKAIKEAVH
                     IPIVGIGGISTENAASVKAAGADGIAAASGILGSENIVEAARRLKGL"
     gene            50088..50525
                     /gene="mscL"
                     /locus_tag="PKI50_04155"
     CDS             50088..50525
                     /gene="mscL"
                     /locus_tag="PKI50_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNT02940.1"
                     /translation="MWKEFKAFAMKGNVIDLAVGVIIGGAFGKIVSSLVNDIIMPFVG
                     LLIGKLDFANLFITLGDGSFNTLAEAKEAGVATLNYGLFINNIIDFLIVAFSIFLVIK
                     QINRIPRKKEEPAPVQTKSCKYCFSEIHIDASKCPHCTSDLND"
     gene            50722..51819
                     /locus_tag="PKI50_04160"
     CDS             50722..51819
                     /locus_tag="PKI50_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02941.1"
                     /translation="MKKVLSIVLLLTLALSVLAGCSAPAAPAEPAAPAAEGGSIVKLG
                     IGHITTIAKSKDLGKDAEGKEVLPLGQVDTVMAAVGFDKDGKVVSVTIDNAQTKVTFD
                     AALQVTSDIKAESKTKVELKEEYGMIKASSIGKEWYQQAEALGQWMVGKTVDEIKAMK
                     TKNKDASHPAVPDVPELTSSVTVSVQEYIAAVAEAYETAVDVAGGEKVGLGHVITTGK
                     SKGLGKDAEGKEVLPFAQVDTVMAATAFDKDGKVVGTIIDNAQTKITFDNTGKVTADK
                     AAEYPTKVELKEGYGMIKASSIGKEWYQQADALGQWMVGKTVDEIKAMKTKNKDASHP
                     AVPDVPELTSSVTVSVQEYIAAVAEGYANAK"
     gene            52004..53098
                     /locus_tag="PKI50_04165"
     CDS             52004..53098
                     /locus_tag="PKI50_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNT02942.1"
                     /translation="MKKTSVIVITLVILLLVLSGCGKAPELVLTKYSDTFFGTFDTLT
                     QVVGYNKSEQEFSEYFEKIRARFEELHKLYDIYNDYEGINNIKTINENAGIKPVKVDK
                     EITDLILFAKEWHAKTGGRTNIAMGAVLSIWHDYRERGEGDPVNAELPPLEKLREASN
                     HTDINKVIVDEANSTVFLSDKEMRLDVGAIAKGFATELVAKEIMSEGFTSGIISPGGN
                     IRVLGKPLDGMREYWGIGIQDPDKMFIAAEENLLDTIFVNNAAIVSSGDYQRYYVVEG
                     KIYHHLIDPDTLMPGEYYRAVTIVTEDAGLADFLSTTAFLLPYDKSRKLVESLDNVEA
                     VWVMPDGKVEATDGMKKLMKSHGATGSKAE"
     gene            53172..54443
                     /locus_tag="PKI50_04170"
     CDS             53172..54443
                     /locus_tag="PKI50_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNT02943.1"
                     /translation="MEVLTLGSRGAAVKLIQSLLIRIGYNPGPVDGIFGPVTREAVRE
                     FKLDNGLEPDGVAGPAAWSRFERFLRGYDTYTIRPGDTFYNISGRYYTTLNAVMTANP
                     GVDPGNLRVGQVINVPYGIDVVFTDIDYTYEIMDRDIRGLKVRYPFIEVGIAGRSVLG
                     KNLYYIKLGNGPSEVFYNGAHHALEWITAPLLMKFIENYARGYARNSGIQGYNIRDLW
                     NRGSIYIMPMVNPDGVNLVLEGLKRDNPYYDRLLAWNDTGLPFSQVWNANIRGVDLNR
                     NYPASWEEAKAQEPSLGVDGPGPTRYGGPSPLSEPETQAVVNFTRQHNFKLVIAYHTQ
                     GRVIYWLYKGIRPPRALEIANIFSEITGYAVSGVPYEAAYAGYKDWFVEQYRRPGYTF
                     EVGIGRNPIPISQFDTIYRENEEVLLLAPIV"
     gene            54687..55436
                     /locus_tag="PKI50_04175"
     CDS             54687..55436
                     /locus_tag="PKI50_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HNT02944.1"
                     /translation="MSSGKKEFFEKDLYKPIYDYLAGLGYTVRSEVNHCDIAAIKENE
                     LLVVEMKKALNLDVILQAVQRQKLADMVYIAVPKPGRGLFSKRWQNLCYMLKRLQLGL
                     MVVSLKEGCSFVEVVFEPAAFDMAKSKNHSKRKRQELVKEFAARYGDFNTGGSTREKL
                     VTAYREMAIHIACCLMRYGQLKPRELRQLGTDEKKTTAILQKNHYGWFENISKGLYAI
                     NDKGRQEVSEYTELYQYYMHLAEEKSESEDK"
     gene            55438..56667
                     /locus_tag="PKI50_04180"
     CDS             55438..56667
                     /locus_tag="PKI50_04180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT02945.1"
                     /translation="MNKTTKALISLGVNCLLVIIAYFVSVWLKAAPPTIKLLSQYVSY
                     VLFAAAMFFGWWFNRSRVFFCSLTLMLCQLALFQLIPQEPDAAEYFYMILSMTSVYIP
                     LNILVFSIQRERGIASSWGRMRLLFIAAELLIGAWIIWYNDSDLLQLFNIKLFPADIS
                     FIPDLSHMSLLLYFITFIFLFGRQAVTGKNHDTPFMGITLFSFAALMFNKDLNTASVM
                     FALSGLMLSAVVIRASHSMAYLDELTGLPSKRALKEEMLKLGSKYTIAMLDIDYFKKF
                     NDTYGHDVGDDVLKLVAACIKGVGGGGKAFRYGGEEFTIVFPGKTAEEAIPYLEELRE
                     YIANRGFIRRSKSRPKEKPADIVTRSSSYKKLYLTVSIGAAEKNEKYRTSEEVIKAAD
                     KALYNAKSKGRNCVCKS"
BASE COUNT        17284 a        10481 c        13451 g        15496 t
ORIGIN      
        1 atcatcgacg gagcccagct tagcggtgta gtagacggtg atagtgtaac attgggagat
       61 catactatgg gtgtctttgc acaagctaag gttggaacag gcatcggagt aatcacatac
      121 atgactataa ccggtgatga tgtcgaaaac tatacaattg ctcagccggt acttacagct
      181 gacatcacaa ccaaggaatt aactataatc aatgcagcag cacagaacaa gatatatgac
      241 ggaactgata atgctgtcat tggaggagcc cagcttagcg gtgtagtaga cggtgatagt
      301 gtaacactgg gaaatcatac tgtcggtacc tttgctcaag ctgcaatcgg aacaggcatt
      361 ggagtaacca caaacatgac tataaccggt gatgatgccg ataactatac aattgctcag
      421 ccggtactta gtgccaatat cactgccaag ggattgacgg taatcaatgc agcagcacag
      481 gacaaggtat atgacggaac tgctgatgca gatattataa atgcagagct tagcggtgta
      541 gtagacggtg atgatgtaac attgggaaat catactgtcg gtacctttgc tcaggctaca
      601 attggaacag acattggagt aaccacaaac atgactataa ccggtgatga tgccggcaac
      661 tatacaattg ctcagccggt gcttgcaact gcaaacatca cggccaaaga attgacggta
      721 atcaatgcag aagcacagga caaggtatat gacggaactg ctgatgctga tattataaat
      781 gcagagctta gcggtgtagt agatggtgat agtgtaacat tggaaaataa tgatgccggc
      841 acctttgcac aagctacggt tggaacaggt attgaagtaa ccacaaacat gactataacc
      901 ggtgatgatg ccggcaacta tacaattgct cagccggtgc ttagtgccga tattacggca
      961 aaagaactga ctatcggcgg tacctttacc gctgaggata aaatatatga tggaaacaca
     1021 actgttgtaa ttgacgattc agacttgaca ttgacagggg ttgttgacgg agatagcgtt
     1081 gtactgaatg ccgcagcagc ctttgccgat gcgaatgcgg cagattatat tgtagtcagc
     1141 ctgaccgatg cttcatcaat aagcggagaa gattcaggca actacagcct gtccctctta
     1201 ggtgcaccca caaccactgc tgatattact ccgccaccga gtgattccaa agccataact
     1261 gattttagcc tggaagcatt cgaaccggat atacaggctg tcatagatga aggagcaaaa
     1321 accataacca tggaagtacc attcggtacc gatgttacag ccttagtacc tactattgaa
     1381 cataccgggg ctgatattat tcctgcttca ggtgtagtcc aggatttcac caaccctgtg
     1441 acctatgagg ttactgctga gaataatact actgcagttt acacagtgac aatgagtgtg
     1501 gcattaccca gtaatgaagc gtgtttgacc aacttagtaa ttagcagcgg cattctcaat
     1561 ccggcatttg taccggacat acaagcatat acagccgatg tggccaacag tacagccgat
     1621 ataacagtta catccacagc tgcacacccg ggagcatcaa tagaggttaa catgacggca
     1681 gtagttagcg gagaagcttc tcaggcaatt gctctaaatg taggagttaa tacaatcaac
     1741 ataattgtaa cggctgaaga tggaacagaa agaatctatg aagtcactgt gaacagagca
     1801 tcatcagata gtgacaacga cgatgacaga gacaatgacg atgacaacga cagaaataca
     1861 tcgatatctg aaccggagcc aactacttct tcaaatataa caataaatgt taatggaaac
     1921 tccataacct cagctgcttc agtggatagc gaaacaggga ctgtaacagc ggttttgaat
     1981 gaaagtgcca ttaacaatgc attgacagag tcacaggatg atggacaggg agttgataca
     2041 gtagatatta agataccaaa aacaacagga gccaactatt atgtaacaga gcttcctgca
     2101 actgtattaa ccggtaacaa tacaacacgg atagaaatta atagcgagtt ggggacaata
     2161 accttaccgg gcaatatgct gggcgatgag ggagcttatg gtattgacaa tgtaggcttg
     2221 agtataggaa cggttgataa atcagctatt gacgaaaaag ttcgaacaca gataggtgac
     2281 aaacctgtta ttgagcttaa gcttaaatca aaggaaggca caatttcatg gaataatccg
     2341 gacgcacctg ttacagtatc aataccttac acgcctaccg agaaggagct ttctgacccg
     2401 gaacacatta cggtatggta tatcgacggt gaaggtaatg ttattgaagt tccaacggga
     2461 aggtataacc ctgtcacagg tatggtaacc ttcagtacaa cccacttcag taattatgca
     2521 gtggtatatg tgacaaagac ctttgatgac ttagagaatg cagcatgggg gaaaaagtca
     2581 attgaggtac ttgcgtccaa aggagttatg aagggtgtat ccgaaaaaga atttgaacct
     2641 caatcaaata ttacaagagc agacttctta tattcgctga taagaacttt gggtgtagaa
     2701 gcaagattcg atggaaattt cgatgacata agtaaggatg attactacta caaagagata
     2761 ggaatagctc aaagaatggg tattactaag ggaacaggca ataacaaatt caatcctgaa
     2821 gaaagtatta ccagacagga tatgatggta ttgacagaaa gagcgctgag aatgtttaaa
     2881 aggatagaag cacagggagc agcttcggat cttgacaaat ttgctgacaa atcccttata
     2941 gcagattatg ctgtaaacag tgttgcttct gttgtaaagg aaggattaat tgtaggcagc
     3001 ggtgatggtg taaatcctct tggaaatacc acaagggctg agacagctgt attcctttat
     3061 agaatatata acaagtagaa gaacttaaag ctataagaaa aaagcattgc gttattaaac
     3121 gcagtgcttt tttctacaaa gactcaaagc tccagtaact gttttagaag cttaatatta
     3181 gtcctgctaa ccggaatcaa atcgtttgtg tctgtcattc ttatatcaaa ggaataatta
     3241 atttttggaa taatttctgc aatcttgtcc agattaacca gatagcctct atgggtgcgg
     3301 aaaaaatcaa ggttttttaa tttattctca aaatagttaa gtgaatgatt ggaattataa
     3361 aatctgtcct tggtgcgtat acgaacaaat ttattaagtg cctcaatata gtaaatgtct
     3421 tttatgttta acagaataat attatcctgc tcatatataa agaatttctt gacagattct
     3481 ttatattgat tacttaagga atgaatatcg gtattaatgt ttttttctat tttttcttcc
     3541 agcctggaaa tggtcttttt aatccgttcc aaggtaaagg gcttgagtat ataatctaca
     3601 gcattaagct caaaggcatt tatggcatat ttatcaaagg ctgtgacaaa tatgattttt
     3661 acctgagggt gggtggtcat aatttgctcc gccaggtaaa taccactaac ttccggcatg
     3721 tcaatatcca aaaagaccac atcaggattc agcatagtta tattaaagaa gctctccaag
     3781 ggattggtaa atgaaccaat tatctgaatg ttagggagac tttccaattg cagttgaagt
     3841 tcttttaacg cgaacaactc atcatcaact aatattgctt ttatcatgga cattatcact
     3901 ccttagggga atttttatat taatagtggt gcctgcgtca atggtactta caatacttaa
     3961 atctgttcca taggcagtat ttaaacgctg atttatattg gtaagtccgg ttccaagctg
     4021 gctatagtca catttggtta atgccagtat tttattcttt ggcattccta taccattatc
     4081 agaaactgag agtattaggt tcttttcctg gacaaaagct gaaatagtaa taatgcaatc
     4141 ttcagaattc tttctaaaac catgtcttac agcattttcg accaagggct gaagaagaaa
     4201 cggtaatatt ttatgattta atgtctgttc gtctatctgg taatttactt tcaagcggtt
     4261 gctgaacctt accgactcaa tagccagata agactttaca atatccagct cgtattcaag
     4321 agtgacaagt ttgtttttat tatataagtc aaaattaaat ttaccatgca aatatttgct
     4381 tagttccata agtaagctgc gggccttttc aggggattcc ctggtaagat ggatgattgt
     4441 attcagagtg ttatataaaa aatgcgggac aatctgcgct tgcaaaaaag ccatttctgc
     4501 cttggccagc ccttgtgccc gttgaaatac atccgcaata ttcaaggata atgagtgcat
     4561 ctgacaaaga ataaatataa taagtccgat agaaaaggta tatgccaggt cataattatt
     4621 atggagcttg tacacgtaac ccatatcaat caaggcagtt aaaagaagga acaaggtgcc
     4681 gaaaagcagt gtgcctgcac cgggtttttt ctttttggct gctgctgcta ttacaaaaat
     4741 catataaata aaagcgataa taaataaaat taaatataag tgcatcaaag gaccaaaaat
     4801 tttcagcggt gtaaaaatga taactattat gtaaagtgaa aaaacagtaa ctgtagtact
     4861 catgattttc ctggggaatt ctgtctgata caggctgtac aatgataatg ccagaagaag
     4921 cggacctgag ggtacggtta tatattccag ctttgacatt agtttaaagg atatgtccgg
     4981 aaatatcaaa aaaacgacag cctctctgat taatagttct ctaagaccgg ccataaaaga
     5041 aaacatagct aaatacaggc tagtcacatt gctttgcatt acagaaaaaa ttaccagata
     5101 gtaaaaaact gaaataaata atacaccaat cagaataaag cttagtaata aattcaattc
     5161 cctgtgttta taaatcgctt cttcggtgcc aagaattatg gactcccata tgcccccttt
     5221 tacatggtgg aagttagaaa catgaattac tatatccagt tggccggaat cagtatttaa
     5281 agttttaatc aaaggcttcc attgaggggt ttcctcttca atggatttac caacaacacc
     5341 attgtgggat atttcctttc cgtttacaaa aaagcgatag ctgctggaca tgtcaggtat
     5401 ctttaaagcc agtttttcat tgcaatttga aggaagcaag accttaagcc tatatgtacc
     5461 gtatcctgaa gcaccgattt cttctttgtt atattggtag gaattccaca agccgggaac
     5521 agttataatt ccgggagctg aatagtcata attatcaggc ataataaatt gattgtagaa
     5581 aaatgaccat tcaccatcaa gtgaatgtaa tgattcatca tcaaaatgca gatcagtcaa
     5641 atcaagtatt ccctgtttag ctttaagctc aattcccggt acacccttgt tatttttgaa
     5701 gctgttgtac tcaataacaa ttattaaaga tataagggaa attattaata gataaattat
     5761 atagctttta taattcttca tttccttcac ctattatcaa taatcttgac tttgcctgtt
     5821 ttaatcactg aattcttgcc tttctcacat tttagcataa ttcgatataa tatgcagagc
     5881 aaattatcca ataatattgt aaatatttct atatataaac cacaatcgca agattagttg
     5941 ctttttacta tttactgaaa ttattagttt ggaaaactcg tttacggaca acgaataaag
     6001 agcatatccg cctacggagc ggtcatctgt tgctaccatc tccacattat ggtagtatta
     6061 accaagagat gatttttgga taagaaggta gaaatatggc aagattattg tttcaagggc
     6121 atggcagctt ccgtatcata tcagacagtg atgcggtaat ttatttagac ccttatgccg
     6181 gagccgggta tgaggagcct gcagatgtaa ttctggtaac ccatcagcat ggagatcata
     6241 accagattca acttccggca aggaaaagca cttgtgttgt tatacaggag aaagatgcac
     6301 tgcagaatgg ggaatacaag agctttgaca taaagggtgt aaagatccgg gcagtacccg
     6361 cctataacaa aaaccatgac aggagcaaat gtgtcggtta tatcctgaca gtggacggta
     6421 taaaggtcta tgcatcaggt gatacgtcca ctactgaaga aatgaaggaa tatccttcac
     6481 atcatattga ttatgcgctt cttccaattg acggagtcta taatatgaat cctgtagaag
     6541 catccaaatg cgctgatcta attggtgcaa agcatacaat tcctatacat atggcaccgg
     6601 gaatgctctt tgacaggcat aaagcggagc agttcaaggg aaaaaaccga ttaattgtca
     6661 gtgcagggga tgagatagag ctttagcaag acaagggggt ggatatgagt atgattcatc
     6721 gggttttgca gcgtaagcat atcaatgaac agcttaaaag tgtctttgca tatccgctga
     6781 cggtggcagt ggcagccatg ggttatggaa agaccacagc ggtaagggaa tttctcgaca
     6841 gggcaaacgc aaaatatgta tggctatcgg tggagagcga ttacagatct gccggacata
     6901 tatgggattc tcttaccagg cagcttgcca agctccggcc tgatctgggc aatcagctta
     6961 atgccttggg ctttcctgct gatgcttctc aaagagacaa gataatcagt atcattgaag
     7021 aacatacata tctaaatgac atcgttctgg tacttgatga ctatcattat gcccgctctc
     7081 aggaattgga caagctgttg gaaagaatag tacggacaaa tatagaggga tttcacatca
     7141 ttatcatctc aaggacaaaa ccggaaataa atattgagga attaagcctc aagggatact
     7201 gcttcatgat tgccagtgaa ttgttcgagc tttcaagaga tgaaataaga gagtttttca
     7261 agttgtatgg atgcagaatc cctgataata gtgtaaggac tgttcatgag atttccgaag
     7321 gatggattac ggcagtttat cttatccttc ataggtattc cgccacaggc agattggaga
     7381 ccggacggaa tattgaaagt ttgattgaat ctgcgattat gtcaagatat acagaggaag
     7441 aatcacagat tcttaaatcc atatgcatac ttgacagctt taccttgcag caggcagtgt
     7501 atgtaaccgg caatgctgcg gctgcaggca ttattgaaag gatgagcgca ggaaactctt
     7561 tccttcgata tgatgaacag ttggatgtat ataaaatgca taatattttc agtgaatacc
     7621 tgaaaaagca aattgaaaag cagggcagca gcatcgaagc ggaagcatta tacaagcgca
     7681 gcggccagtg gtacatcagg agcggcgata tattgtcggg attaagcttt ttgttaaaag
     7741 caaaggaata cgaccttatg ctgacagaat ttgaaaggca tggggcaacc caggaactgg
     7801 acaaggaccc tcagcttata gtggaggtat tttctcaaat tcccgatgag gtaaagtacc
     7861 gccatccgat gagctattta acttatgcct ataactatat tagcagcgtg gatatggagg
     7921 gcggcgccga actgctggct caaatagaag agcattaccg gtatgatgaa agcattccag
     7981 atgagctgag acggaggatt gcaggggaaa ttgagcttgc cgggagcttc ctgtacttca
     8041 atgacctgga aaaaatgaat gaaaagtatc ttaatgcgta cagcctgctt gacggcaaat
     8101 catccatagc caacaaggac atgatattta ccttcggcac tcctcacaca ctttatctat
     8161 actacaggga aaaggggaga ctgctgtgga tagttgaata cctggataat tatttccact
     8221 atttttgtga actgtccaag ggctgcggta caggctttga agaactggca agggcggaat
     8281 attgtttgga aaccgccaat tttaaccagg cagaactata tgccttcaag tccatataca
     8341 aggcgaagcc catggaacag acctccttaa taatatgtgc cagtctgacc ttggcaaggg
     8401 cgtatgctgc acaggggaaa tttgataagg caagggaact gttagataat ttggcagcgg
     8461 agaccgctca atataataat cctgtttata acagcactct ggatttatgc aaaggttacc
     8521 tgggagggat aacctgccgg cttcaagact ttgctccctg gttggaatcc ggtgatatgg
     8581 agcaaagcga tatattattc ctgggtatgg gattcaatta tatcgtccat gctcaggcag
     8641 tgcttctgca gaaaaactat ataaagctgg aagtgctctg tgaggggatg ctgcagatat
     8701 tctcaatgtt taacaacctt ctgggctttc ttcatactca tattttggac gctgcagcca
     8761 agtataacct gtacggtatt gaaagggcaa aggcagctat gctgcaggca gcggacatcg
     8821 gcagggctga tgatatcata ctgccctttg cagagtatgg tgcgcatatt ttggacatac
     8881 ttaaagccct ggataatgat gagaaaggtg atgaatatat ttcccggcta atatcagcag
     8941 ctgaacagta cagcaccgga ctggaaaggt ttaaagaaga aaaaatatcc gttccttccc
     9001 tgacaaagcg ggaacgggaa gtgctgaagc ttgttgcgga ggggaaaacc aacagggaaa
     9061 ttggggctga actctttata gccgaggtaa ccgtcagtaa aaatattact tccatttatc
     9121 aaaagcttgg tgtcacaggc aggccctctg ccgtaaaaaa agcaattgag ctgaatattt
     9181 tatagttttc ccccaaaagt ataggatctg ataatatgaa atttttttaa accctagtat
     9241 gattatatca ttcaagggtt tattattttt gttgcagatg ggaggggtcg atatgcccaa
     9301 gataatacgg gtagaaccga aggatgacca cacactgctt attgagctga ataacaaaca
     9361 tatgatcgtc tacgacatga agcccaggct ggaaacagtg cgcttttgcg gccttgctga
     9421 ccttaaccgc ttcaaagatg tcaggataga ggatgaaaac acccttgtct gggacagcct
     9481 gtgccagatt actattgatg aagtccttag catgattgaa agatgatagt caattattat
     9541 tggaaaggaa gtgaatgact tgaaaaacaa taataccatc caattgctgg tgaagacaat
     9601 agcaattaca gcgatgtttc tgctggcagc attcctgctg attccggaca cggttttggc
     9661 ggaaggaggg ttcgcgaggg gggacggttc gccggaacag ccatttgaaa tcgcaactcc
     9721 cgagcagctg gcaactgttg cggaagaggt atattgcagt agtcatttca aattgataga
     9781 ccatattgat ttgagcgatt atctatctga tggaggtggc ggctggaatg aaggtgcagg
     9841 ctggaagccc attgggacgt atgacatgtt cgatccaacc aaggctttca ccggtagctt
     9901 tgacggtgat ggatatacca taagcagcct ctttattaat aggagtgata cggagggtgt
     9961 cggcctgttc ggttacacgg ttggtgcaac tattaaggat gtgacattga gtgatgccga
    10021 tattaccggt gatgcttgcg ttggcagtct agtgggggta aatgaaaact gcacaatcag
    10081 cggttgttcc gcagagaatg cggaagtaac cggtttcacc acagtcggag gccttgtggg
    10141 cagtaatgat tctgggaata ttgccaactc attagccaca gctgaagtta gtggggtttt
    10201 ctcggttggc ggcttggttg gggacaacta tggcggttcc atcgaaagct cctatgccac
    10261 aggcactgtg tcgggtattg attatgatga tcccgaaagt cagggacact tcggcggttt
    10321 ggcaggacgt aactatattt acggtactat cgaaaactct tactccaccg gtaacgtcgc
    10381 cggcattcat cttgtcggtg gattagtggg gtcgaataca gatgccggag ttatcgaaaa
    10441 ctcttactct accggtgatg ccgccggaac tgcatatgtc ggaggattgg tggggcttaa
    10501 ctctcttgat gaagaatctt atgataaccc atttattaca gactgctatg ctgcaggctc
    10561 cgtggaagga gaacaatatg taggcggttt ggtgggccag aattgcgaag gcgatattgt
    10621 acactccttt tccatagggg aggtaagcgg tacaaataaa gtcggcggac tggtggggga
    10681 aaatgaagac gggacggtta ctgattcctt ctacgacagc aatacttccg gccaatcgga
    10741 tgaaggcaag ggtgagcgta aaagtacggc tgaaatgaat gcgtatacaa cattttttaa
    10801 tgcctcttgg gattttatag gtgaaatgga taacggaaat gaagaaattt ggggtattga
    10861 cgaatctgca gataaccctg ccaatgacgg ctaccccttc ctgacctggc agggctatgg
    10921 ccataaagta tggcctgcgg tatctacaat ggaagcggag gatattacag atataactgc
    10981 tgtcggacag gggggaatta ttattattgg ctatcccaat cccacacagc atggggttgt
    11041 ctggagcagc catgaggatc cgacactgga agataataaa acagaaaaag gagcagtttt
    11101 gggagccgga tcatttacct cggaaattac cgggttagaa ccggaaacta cttactatgt
    11161 aagagcatac gctacaaatg acttgggaac agtttacgga gaacaaatca gttttacaac
    11221 agaggaggga acttttgccg gcggaaatgg attgcctgag aatccatacc aaatacagac
    11281 tgcggatcag ttggctgcgg tatcagagta catggacagt tattttgtcc tggtggataa
    11341 tattgacctt tccgcttatg gggacggata tgatggcggc aaaggctggc tgcccatcgg
    11401 gaactatgat aattccttta caggcagttt tgacggcaac ggacacacca tcaccggact
    11461 ctacatcaac cgtcctgata catattacgt ggggatattc ggctgtacgg gagcggatgc
    11521 tgagattagt aaccttaggc tgctgaacgt ggatgtgtcc ggctatgggt atgtcggcgc
    11581 tctggtagga gaaaatcatg gcactataac cggttcctgc gctgcgggca gcgtggaggg
    11641 cagtgattat gtcggaggct tggtgggact gaactgggag gctaccggca acatcattga
    11701 ctcttattcc gcagttaatg tggttggggc agagagtgtc ggcggcctgg ccggaggaaa
    11761 cgaaggcacc ataaccggtt cccatgccac gggggacgtt gtgggcgagg attatatagg
    11821 cggactgaca ggaggcagtg ggaccagtga ttacaggtgt tccattactg actcctatgc
    11881 tacgggtaat gtcaccggta acggcatagt tggatatgtt ggagggctga cgggtcaaaa
    11941 ctttaatgct gatattgaaa attcctatgc tgtgggaacg gtaacgggaa gcaataatgt
    12001 cggcgggttg gtaggaataa atgaggcaga cggtactgtc acaagctcct actatgatac
    12061 ggaaaccacc gggcaaaccg atgggggcaa aggtgtaccc aagaccactg cagaaatgaa
    12121 gcagcagggt tcttttgtag gttgggattt tactgatacc tggaatctag ccggaggcac
    12181 ttatccgtat ttggaatggc agacagataa ttttgatttt gctccgcctg tatttactgt
    12241 gggttatccc aaggcagagg acatcaccta tgaaagaatg gacatactgg tgcaggtgga
    12301 tgaggttgcc gctgcatatt acgtggtatt ggctaatgga gtagatgcac ctgatgctgc
    12361 ccaggttaag gcaggcatgg attctgaaaa tacagtctta gctgaaaacc tcaagggaag
    12421 cattgcgggg atatccgcag ggacagacgg aagggcagtc attaccgggt tggctccggg
    12481 aacggaatat gatatctacg tggtggctga agacaatgcc ggaaatatgc agcctgacgg
    12541 cagcgtggca ttgttagaag catgcaccgg gcttatgccg accggcaata tgagttttgc
    12601 tctgacaaca ccggttccgg aggctgacgg aaacagcatt gcagttgacc agtcttctcc
    12661 taaaattgtg gatgtggaca ctgtaaacgg catagggtcc gtggagatta ccggaacgaa
    12721 aacagcggct caaagcgtga ctgtcggcgg agttgatgca gataaagtaa cggcagccgg
    12781 agaagccaca gaaccggttt atactataga tacctccgct attgcggcag aaggaggcac
    12841 atacgaattt accctcacag tgagtgaggc aggcaaagct gatattgttt acaatgtaac
    12901 ggtaacagta gctgtgcctg acaatacccc gccggtgccg ggaaacggcg ggaccataac
    12961 cgtttcaaat attacccata acagtcttga actgagttgg gcagcggcat cggacgatgt
    13021 aaccgcacag cagaacctgc aatacaaagt ggtccgttcg gaaagtaata atatagatac
    13081 ggcaattaat gccgaatcaa acggaacaat cgttaaggat tggacggccg ggcttacggg
    13141 tacggaggca agcggcttaa gcgcggaaac catctattat ttcaatgtga ttgtgcagga
    13201 tgaagccggc aatatggctg tgtacaccat gaaaagtcag gctgccggat cagttcctga
    13261 aagggaggat gaagacgata gagatgaaga tgatagaagt gaaagaaacg tttccggaaa
    13321 ttcgcctgtt tacaatgctg tcattaaagg aagcaatacc tcggggacca cgattacagt
    13381 taatgtcaat acccggagag gcagtgcagt agtaaatctg ggggaacagt tcgaggatgt
    13441 ttcaaacggc gaagaagttc ctctcatcac tataccttcc attcccggag ttaatgctta
    13501 caccgtagga attccagctg actccctttc cggctctcaa agtaaagaca tcctgacctt
    13561 ctccactcct gaaggcagca ttacccttcc cggaaacatg ctggcgggta ttgcacaaac
    13621 cggaagcaaa ggagccggta ttaccgtagg tgagggtgac aaatccctgc tgcccgatga
    13681 tgtaaaagca gccttgggcg acaagccgct ggtgcagctc tcattgacac tggacggaaa
    13741 tcaggcggag tggaacaatc ctgatgcccc tgtaacggtt tccataccat ataagccgac
    13801 agcagcagag ctttctgacc ccgagcatat cacagtatgg tatatagatg gcagcggcaa
    13861 tgtggtggaa gtaccctcag gaagatataa tcccgaaacg ggtatggtta ccttcagcac
    13921 aacccatttc agtcattacg ctgtagccta tgtaacaaaa agctttaatg atctgggaag
    13981 cgcggcctgg gcaaagaagc ccattgaagc ccttgcatcc aaagggatat tgaagggtat
    14041 atcagaaaca gaatatgccc ctcagacaaa catcacaagg gcggacttcc tgtactccct
    14101 ggtcagggca cttggtattt atgccaaggt tgaaggaaat tttgatgata taagccgcga
    14161 tgcctattac tacagagaga tcggtacagc caaaaagctg ggcattaccg gtggtacggg
    14221 aaacaacaaa ttcagccctg atgaaagtat cacaaggcag gatatgatgg tattgactga
    14281 gagagcattg agaatgctta agaagcttga agtacagagt tcagcttcag accttgacag
    14341 gtttgccgac aaaacactga tagcggcata tgccacagac agcatagcct ccgttgtcag
    14401 ggaagaactg attgtaggca gcggtgatag gataaatcct ctgggcagca caacaagggc
    14461 ggaggcggcg gtattcctgt acaggatata caataggttc taatataaga cacacctctt
    14521 caggaacaat gttcagggca gagggatatt tcttcatatg atagtgtaat agaaaggtgc
    14581 ggctttgtcc gcacctttgt gctgcgataa aataatagta aacattaaat aatttatatt
    14641 gtataattgg cttataatgc ttgaaatcta aattatgagg taataacaca tgtctgaatt
    14701 caagaataaa attacttttg caatttacgg catagtcttt catatcctta tttaattgta
    14761 gtacttgtat aatttcaatt aataatgtaa tataattata taacaaatgt tatataatga
    14821 actatttcct tacttcgtta aatcttgtaa taaagggatg aggatactat ggggcctaag
    14881 ataaaattca caagggagca aataattgat gcagcctttg aaatagccag ggcagaagga
    14941 attgacaatg taaccatgag aaaaattgca cataggatgg gaagctctgt ggctcccata
    15001 tatgtaaatt tcaagagcat taatgaattg attgaagctt tgatagaaaa aattgcagat
    15061 atcggccagc agcttattgc tgaagaaaat acgggaagcc cgtttgctga tatcggaagg
    15121 gcaagtataa gatttgcaat ggagtatagt gtccttttca gagatttggt aatgaaaaac
    15181 agcagatatt tgaaaggcta tgatgagaaa gtcatgcccg cattaattga ggaaatgaag
    15241 aaggattcag atctggaagg tttttcagag gataagttaa aagaaatatt gttaaagatg
    15301 agaatatttc aactgggact ttcggttatg gccgccagcg gtatactatc aaaagattat
    15361 gatatgaaag atttgaccga tttattgtca agcactgccg atgatgtaat actatccgca
    15421 aggctgaaat aaaaacatca gaattcttta atatcgcacc ctaaggcatt tttacagtca
    15481 gaaggggagc gatatttttt attgcatttt tctaaaaaac cccttgcaat aatttcggtt
    15541 tcagtgtaac atttattata taactaatgt tatataataa atgttttata aaaaatttca
    15601 gcttgcctgg agtaatagag aggggaaaat gagcattgga taagatagtt attatcggcg
    15661 ggggcattgc agggttgagt gccggcatat tcgcacaaaa aaacggcttt gagagtgtga
    15721 ttttggaaaa gcacaatgtt ttaggcggtg aatgcaccgg ttggaaccgt cggggttatc
    15781 acatcgacgg atgtattcac tggctggtgg ggacaaaaaa ggggactccc attcacgatc
    15841 tctggactgc cgcaggggcc ttggacgggg ttgaaatcta ccatccggaa agcttcctgg
    15901 cttttgaata cgatggcata agagtgcacc tgtaccgcga tcttgaccgg ctgaaatcca
    15961 gctggcttga attatcaccc caagacgaag ctgctatcaa taatttttgc tgcaccataa
    16021 agcagctgca atcttttgaa ataccggtgg gaaaaccgga ggacatgatg gaatttatgg
    16081 aaaagataaa attaatgatt tcgttattta ttcctgggcg gcgatataga agccccctgt
    16141 gaggcagttg aattgaatat tgaaggcaat agggtccgcc gcgtcatttg cagcagcggc
    16201 aaagcctttg aagctggtta ttacattgct gcctgtgatg cacacgtgct gtatgagaaa
    16261 ctgctgaaag gaaagtatcc cgatcccgaa tttcagaagc ggttcaataa tcctgaagat
    16321 tatcccttag cctcggaaat tcgtatagca ataggttatg aaggcaggac ggacgaaatt
    16381 ccgcgcacgc tgcgattccc gattgattcc tttaaagtta atggaagacc aattaattac
    16441 ttgacaataa ctcattacgg ctatgaaccg gactttgccc ctgaaggcca cgtcttaatc
    16501 acctgcagca tcaaccaatt ccatgctgat tatgatgcat ggaatgcact tgctgcccac
    16561 cattaaagga aagatgatgg ctcatacagg gcgtataaaa ggtttggaaa atatattttt
    16621 aagcggccag tggctgcagc cccccggagg attgcttgtg gcactgatta ccggaaagga
    16681 tactattatg aggctttgta agatgaaaaa caagttgttt gtatactaaa gaacaggtgg
    16741 tataaaatga ttatatatta taacgaatat aataagtata aaaataattt atatgacgaa
    16801 ctaagggaat attgtctatg tgaacgggca aatgaagagc atctgagaac attgattcat
    16861 actgattgcc atcatattcc gcaatggata agaacattat ctttgaaagt gaagctctgg
    16921 cctattgatt taagataggt atttctctat aattgtactc acaggctgca ttatgcttaa
    16981 ttaatataaa ggggtaatgc tatggacggt aaaaaggaag gtttacaagt taagaatgat
    17041 gtttcagggg tggtatacgg ggtaattgct tatacagtat gggggctgct tcctatatat
    17101 tggaagctgc tgggggaaat ccctgctgat gagattttag cccatagaat attttgggca
    17161 tttttatttg tcgggggatt atttttatcc aaaaatgata ttggggtact taaggaagca
    17221 attaaggaaa gaaaaaatat aagaaatata cttatttgtt ctttccttat aacaataaac
    17281 tggggtactt atatttgggc agttaattca ggcagaattc tggaatccag catgggttat
    17341 tatattaatc cattaatggt ggtactgctg ggaatgacag tgctaaaaga aaagctcaat
    17401 gtacttcagt atatatccat agtatttgcg gctgcaggag taattataat aaccgtacag
    17461 tttgggaata taccttgggt ttcacttctg ctggcattaa gctttgcatt gtatggctta
    17521 tttaagaagc ttctcaaggt tgaatcctta acgggtatgg tattggaaac agcagcaatt
    17581 atgcccttgg cgttgggtta tatcctgttc aagcttttca gcgggcaaag cgccctatat
    17641 tcggtaccct tatcaactgc tgcaatactg atgctttcag gaatagcaac agctttgccg
    17701 ctgctctggt ttgcaatggg agcggccagg ttgaagcttt cgacattggg tttcctgcag
    17761 tacatatctc ccacattaag ccttttatta ggagtttttc tttatggaga gaagtttacc
    17821 gtcactcatt tattaagctt ttcttttatt tggacaggcc tcataatata ttccttttcc
    17881 aattttgaag caaagagcaa gtttgggaac agcaagcaag ctgatgtata agagccataa
    17941 atacatttca tagaaggctt tcttggttgt gtaaaagaaa gcccttttta tttatatttg
    18001 acaattatag ttatatataa tattatatat acaatatata tattatatat cataaataat
    18061 attatatata tggaggagca ctatggaagt agctgaattc aaaaacattg ttttcgatta
    18121 tacaaggaag atcaatgaaa gcttgcattg cgcctttggc cctgccattg ggaatagagg
    18181 gctgaccatg atgcagacaa gaattctcat ggagctgcat cgctgcgaat cccacacaat
    18241 aggaagcctg gcagacagta tatgtgctgc cggcgctaat atttccgcaa tgtgtaaaaa
    18301 gttggaggag cagggcttta ttgagcgcat cagaaaccgg aaggatgaaa gggtagttcg
    18361 cgtggttctt acaaggctgg ggaaagagac tgtattggaa attgacgaat tgtttaatga
    18421 aaggatttca aagtgcttga agtatgaatc ggaggaggct tttgaagata ttatccgggg
    18481 catgcaaaaa ctaaatgaac tgctgacaag aataataaaa aatgaatgag gatgaattta
    18541 actgatgtta aggagaggac aagcatgaaa gaacagaata atcccagaaa gccgattatt
    18601 tatttttata tcgttacatt ggtaatagtg atgctgctga atgcatatat ttttccggct
    18661 gttgtgaaac agcaggttac ccaggtggac tacggaacat ttttgacccg gattgacgag
    18721 ggaaaagtaa aaagtgtaga gattcaggat aatcaaattg catttaccgc aattgatgaa
    18781 gccgggaaag agaatatata tgttaccggg aaggtaaatg acccggagct tgtagaccgg
    18841 cttcttgcag ctgatattga agatttctca caggtcattc caagggaaaa ttcccctctg
    18901 atgaacttta ttatatcctg ggtcctccct ctgattttct ttattggaat gggacagctg
    18961 cttgcaggca gtatgcagaa aagaatgggc agcaacgcta tgacctttgg aaagagcaat
    19021 gccaagatat atgttgaagc gcagacaggc aaaacctttg atgatgtggc cggccaagat
    19081 gaggcaaagg aagctctgtc ggaaatagta gatttcctgc ataatccggc aaagtacaag
    19141 gaaatcggtg ctatcatgcc aaagggtgca ttgcttgtag gacctcccgg taccggtaag
    19201 acacttcttg caaaggctat tgcgggagaa tcaaaggtac cgtttttctc aatttcaggt
    19261 tccgaatttg ttgagatgtt tgtaggtatg ggtgcggcaa gggtgcgaga tcttttcagg
    19321 caagcacagg aaaaggcccc ctgtattgtg tttattgatg aaattgatac tatcggaaaa
    19381 aagcgtgaca gcggaggaat cgcaagcaat gatgagcggg aacagacatt gaatcaactg
    19441 ttggctgaga tggatgggtt cagcagcgaa aagggagtgg tgatacttgc ggcaaccaac
    19501 agaccggatt cccttgacaa ggctttgctg cgccccggca ggtttgaccg ccgtgttccg
    19561 gtggaactgc cggatcttgc aggacgtgag gcaattctga aggttcatgc aaaaaaaatc
    19621 aagttaggga atgatatcaa ttttaatgaa atagcaaggg caactccggg tgcctccggt
    19681 gcggagcttg ccaatataat aaatgaggcg gctttaagag ctgtaaagtg cggccgcaaa
    19741 tacgtaaagc agatcgatat ggaagaagcc gtggaagtag taattgcagg atatcaaaaa
    19801 aaaggtgcgg ttatatctcc gaaggaaaag gagattattg cctatcacga aataggacat
    19861 gccattgtgg ctgcaaaaca gactgaatct gcaccggtcc acaagattac aataataccc
    19921 cgtacttcgg gagcccttgg ttataccatg caggtagccg aaaacgaaaa cgtgcttatg
    19981 accaaagaag aagctcttaa taaaattgca acctataccg gcggacgggc tgcggaggaa
    20041 ctggttttcg ggacttatac ctccggagcg gccaatgata ttgagcaagc tacaaaaatt
    20101 gcaagagcga tggtcactcg cttgggcatg agcaaagctt ttgatatgat gggactggag
    20161 acaatatcca atgtatatct gggcggtgat ccttccatga catgttcttc ggaaacagcc
    20221 tccaaaatcg acgaagaggt attggcaatt attaagagcg ctcacgagaa agcgataaat
    20281 attttaaagg aaaatatgga gaagcttcac ttattgtcct catacctgat tgaaaaagaa
    20341 acaataacag gtgaggagtt tatgagaata ttggaaagct aaggctgaat aaagaggaca
    20401 cgggggagga cacggggacg cggggacatg gggacgtttc ccctgtccca ctttcagtgg
    20461 gacaggggaa acgtccccat gtccccgcgt ccccgtcgca ttattcttca attgtgcaac
    20521 agttcctttg atctagcctc aaatcacaag tcatgtgaat ataatgtatt gtagtttgat
    20581 gacttgacga ggtgaggcaa agtgaatgca ttaaagcgta tatcccaggt ttttgaagca
    20641 tcggagcaaa ttacttttga tgattcctcc aaaattattt taatgagtga ttgtcacagg
    20701 ggagacggaa gctgggcaga tgatttttcc aggaaccaaa atttatattt tactgcctta
    20761 actcattact ataaaaataa ttttacttat atagagatag gtgacggcga cgagctttgg
    20821 gaaaacaaca atttttttaa tatcaagcag gttcattgca atgttttctg gctgctgtcc
    20881 aggttttaca gtgagggcag gctccatatg atttatggga atcatgacat ggtaaagaaa
    20941 tacaataggt ttgtcaggag caatatgcat cggtatcttg atgagcctgc gagaaggcat
    21001 atgccgttat ttataaatat aaaattccat gaggggttga ttctgaagta taggcctacg
    21061 ggcggcaaga ttttcctgat tcacgggcat caagtggatt gtctgaatga cagactgtgg
    21121 aagttagcca gatttttagt caggtatttg tggaggcctc ttaataacct cggagtaaat
    21181 gaccctacaa gaaccgcaaa aaattataaa aagaaggaga cggcagaaaa gaagcttacc
    21241 gaatgggtaa taaaggaagg gcatgtgctt atagccgggc acacccatag gccaatgttt
    21301 cctgaagtga atgagccccc gtatttcaat gacggaagct gcgtacatcc tcgctgcatt
    21361 accggaattg aaattgcaga gggttatatc cttcttgtga agtggagtgt aaaaactaag
    21421 gatgacggta cattgttcat aggcagggag gtacttgaag gcccaagaaa gctggttgat
    21481 ttttttgcct gcaaagacag aacgaaaaaa cttttgttat gacagaatga aataaggtat
    21541 aattcagtta agataccttg agcaggctaa tggaaaggaa tcaaccatga aaagattggc
    21601 atatgttttt gttatctctg cggcagtgct gtggggtacg ataggaatat acagcaatca
    21661 attaagccaa tatggagcca atgtgctgca gatagtttca atcagagcta tttttgccgc
    21721 ccctgcaata tttgttttta tcctattaaa ggatataaag cttcttaata ttgatattca
    21781 cgattggaaa tatttttttg gcaccggtat tttaagcttt gtatttttta actggtgcta
    21841 ttttactgca attgataaaa ccactttatc tactgccgca atattattat acactgcacc
    21901 tgctattgtt atggtttttt ccataatatt gtttaaggaa aagctgacaa gaagaaaggt
    21961 cgctgccctg attcttacct ttatcggttg tgcatttgtt accggtgttg taaaaggaag
    22021 tcaggatatc tcgccactgg gcatattggc aggcctgggt tcgggatttg gctatgcact
    22081 gtacagtgta ttcgggagat atgcgttgaa gaaatatgac cctataactg ttactctcta
    22141 tacctttgta ttcgccagca ttggtttaat acccataagt gacataaagg ggcttttcgg
    22201 cttattgagc agcaccgttg ccttttacaa tgcagtttta ttaggcctgg cagctacggt
    22261 tatgccgttc cttctgtaca caaaagggct ttcctattta gagacaagca aggcttcaat
    22321 aattgcagca atagagcctg ttgtggcaac aattatagga atcattcttt ttggtgaacc
    22381 gattaccgta tttaaaataa tgggagtatt cttggttgta tttgcagtgt ctattctgga
    22441 acagaaaaaa agctgataag atttattgtt ttataagctc agtttgctga caaagctcaa
    22501 aataagaaca gtaatatata gtcagagcat acttatacga aaattgttaa tggctcttat
    22561 gaggctcgac ttagaaacaa ccgtatgcga ggcatggagg ccgagcaagc tgtctcggtc
    22621 acggacgacc tttgacagcg ctaggttttt cttagttgag cggaattaga gccatcaatt
    22681 tttgtatacc tatgcgagac tataccttac tgttcttata tagaaggttt ttcatcagtc
    22741 tcagctgata agatttgtta tcttatcagc tttatttcag aataggatcc tgctgctgac
    22801 atcttcaatg gaattgcagc cagtcagtat cattgcagat tttaagtcag ctgcaatgct
    22861 gtcaatatag gcttttaccc cttctgcctg tccgccgaat gaagccggaa caaaaggcct
    22921 gccaatcaaa acagcatctg ctccaagggc aagcatctta agaacatcta ctccgtttcg
    22981 cacgccgccg tctgccaata tggtgatttt ccccttcact ttttttgcaa tttcaggcaa
    23041 tacatccgct gttccgggag tttgatcaag tacccttcct ccgtggttgg ataccactat
    23101 ggcagctgcg cctgattcga cggcaagctc agcctcatcc ggagtcataa ttccctttaa
    23161 tattaagggc agttttgtac tttctgctat ttcctttata tcctcaacgg attttggccc
    23221 aaccggcttg ccaagcagcg ataaagttat taaccctgct gcgtcaatgt ctattccaac
    23281 tgcataggca cccgcttttt ctgccatgcg tattttttca atgaccttat catttgtcca
    23341 gggctttatg attgcaattc cttctccgcc tgattttttt agctcttcga gattagttac
    23401 caaaagcgag tctattgctg catctccgaa cattggatag attccgccct gaatacagcc
    23461 ctttattatc catgatatat attcctgttc ctggaatttc cctcccatat tgaaattcgt
    23521 tcctgcaaca ggtgccgcaa aaacaggtat actcatcttt ttcccaaaaa gttcaatgga
    23581 agtgtcaggg tttttacagt tatgcaggac tctcatatta agcttatatt tctttaaagc
    23641 atcgacattt gctgtaaagg cttcgctggt tccctttcct cccattccgg gcacttctcc
    23701 ggaacaagct tttccgttgc atacagggca gactctgcat gtgccgttta aatttgcctt
    23761 ggcatttttt aacacttcat cataattcat aattcgttta cctcttatca tcatgatatg
    23821 ttgatataaa taattcaggg gcttttgccc ctgaattatc ttatttatta agagaaaggt
    23881 ttaaagtaat tttttaagca tctacattct ggttcttgga cttatctccg gagaacgtct
    23941 ttattgtagt tatgattaat actattgcaa gtacgaaaag aattgcggct aatgaaccga
    24001 ttatcacatt gccgcccgca aagttggttc ttgctaccag tacaagggca gtaagggtta
    24061 cgcagaacat aaataccatt gggattatag tcatagtatt ctttttccct gacttagcca
    24121 gccaagcaga tactgcaagg agtgcaagtc cggcaagcag ctggtttgca gaaccgaata
    24181 tcggccatat cttcgtataa ccgtaggaca gcaataatgc agacaagcct acagttacag
    24241 cagttgctgt atacatattg ttcagaggag attttgttcc atccgccttg gtagtcatct
    24301 cctggaacat ataacggcct aatcttgtcg cggtatcaag acttgtgaga gcgaatgcag
    24361 agaatgcaag tgttacaaat acagtaccta cgggaacggg aataccgaag ctgctcataa
    24421 aatctgcaac accggcagcg aagatctgag caggcgtacc tgttgctccg gcaacatagc
    24481 ttaccgcaat aagtgcaatt attgcaacta cgccttcgat aagcattcct ccatagccta
    24541 tcagctgtgt atctttttca ttgtctacct gctttgagct tgtgccggaa gcaactaatg
    24601 agtggaaacc ggaaacagct ccgcaagcaa cagtaacaaa gagcatcggg aaaagatact
    24661 gggccttcgc accctcacca acagtaaagc cgactgaagc cgggattgcc atggcaggtc
    24721 ttactaaaag tactccgatt actgctccgg caagcatagc ataaagcagg aaggagcaca
    24781 ggtagtccct gggctgcagc agcagccaaa ccggcagtgt tgatgctgaa aaaatgtata
    24841 taataagaat tacatcccat gtgagttttg aaaatttcag taccgggaac tgataaccta
    24901 tccatatgca gaagaacagt aatgctaccc ctgctaccgt agatactgaa aggctggctc
    24961 cttttctgta aacaaagtag ccgaagccca ttgccagaaa aataaacaaa attgatgcag
    25021 tacctgccct tgcgccggtt attgcctcag gctttgccgg atcaaaagca aaggttccgg
    25081 cacaaatatc tgtaaaggct gcaacaacca gtattaatgt aaggtatgca aaaatgttga
    25141 aaagcttttt gccgttttca ccgatatttg ctctgatgat ttcacctatt gattttccgt
    25201 catgccttac tgatgcaaag agtgaacccc agtcatggac accgccgaca aatatacttc
    25261 ctactacgat ccacaggtac accggcagcc atccgaatac tgcagcagct atagggcctg
    25321 tgatagggcc ggctccagct atggatgaaa aatgatgtcc aagaagtact ttagcattag
    25381 caggacaata gtcaatatca tccctcaatg tacaggaagg tgtcttctta gttgggtcaa
    25441 ttccccactg attagccagc catttgccgt aaatggtata ggctatcaga aatgccgcaa
    25501 taccgataaa taataatacc aatgagttca taacacgacc tcctttttta tttgcatagg
    25561 aaatattcat ttccctaatg ctctgtttaa tcaaaaaaaa cagttctgaa aaggttttat
    25621 ttattcaaag atgcaaattc atatagtttt tttacttttt tcccgtcctt gttggtaata
    25681 atctcgctat tatccagtcc tacatatata agccttacat cacaccaacc tttaagatag
    25741 aagcaaaacg cttttttata attatacttt tctactgctt taattgcatt ttcatttata
    25801 tgattaccga ttaatactcc cgtaacatat gtgctcatat ggtctttgtc cggctttata
    25861 cattcatcag atattctttt taagaactgc ccaaaagaga taatgtcttt ctcatttaca
    25921 tcatcacatc tcttgatata tatgtgctca tgattattat attggtcaat tatatcatgc
    25981 tgggtaataa atgtccttac acttatattt gaaaacttgg caagtatatc tatcttatta
    26041 tcgaatatca ctatatccct ttcaatggca aaattctttt caagctttga tgtcagcagc
    26101 tttatatact catcgtattt cattataatc ctcccaataa aggcttcttg taaataatat
    26161 gaaatattcc ttaactctat acattcctac aactacatta tatagggaat aaataaaccg
    26221 taatttattt ttgcttgaaa tgacaagaat aatgcattaa atgcaatcat aggcgcataa
    26281 aaaaactccc tcaactgaaa tcctctgaca atcagtgagg gagtcttaaa tacccattag
    26341 ctttttaaat aataatatgt tctgcctgct gaccggaatc tctgcatcca accccttaag
    26401 cttaagcata tatgtattat tgaaccaggg gattatttcc gttattttat ctatattaac
    26461 gatataagaa cggtgacagc ggaaaaaaca atcttgtctt agctttttat gaaaatcgga
    26521 aatgcttgag gcaactacaa agcgcttgtc ctttgtgtac acataaacct cgtgcttatg
    26581 tgcctcacaa taataaatat catctgcatc cagcacatat aatttctcat ttttccaaag
    26641 agttatttta tcgttatgca gcctaacagc agtgctgttc tcaagtcttt gaagcgtatc
    26701 tgctattctt gtttgggagt agggcttcaa aatgtaatca aaggcggaaa cctcaaaggc
    26761 ttcaagggca tgttcgcggt aagcggtgat aaatactaga acaggtttaa ccggcaaggt
    26821 gtttatcact ttgctgaaag ttaatccatc cagcttaggc atattaatat ccagaaaaat
    26881 aacattcact gaatgtcttt ggatgtattc aagggctttt atactgtcat caaaggtttc
    26941 cgatattgta attgaactaa aatttgtaat aaaataactt aattcctgta tggatgggta
    27001 ttcatcatct acaataatac aattcaaaaa atctcacccc cacctgaaca taataactgc
    27061 aggcaatcaa gcactcatat taagcataaa actaaccctt gtgccgacat ccaggcgttc
    27121 aatattaagc ccggtgccat atatatgacg gagccggtta tcaacatttg atataccgat
    27181 tttgttttct ttaacctttc cggcatgcaa attttctatg aaagcctggg gtatacctat
    27241 accgtcatcc tctactgaaa ccctgatttc atccgggtta tgctttttta cggaaattac
    27301 aatatttccg gtgccggagc ccttcagaat tccatgtttt atggaatttt caacaattgg
    27361 ctgtatgaca agtgaaggaa tctttacatg aatgctttca tcaatatcat atataacatt
    27421 aagcttatct ccgaaccgtg ctttttctac ttcaatgtat gatctgacct gctgaagctc
    27481 cttgctgata tctacaaaaa tacttgtttc ttctatgttg taccttaaat aggttgaaag
    27541 gttgacgatt aattctctgg cgctattcgg attaattctt aaaagggcta ttatcgtgtt
    27601 taatgcatta aaaagaaaat gcggattgat ttgggcctgc agtgccttaa tttcagcttg
    27661 tgcagccaat tcctccagct tgccgacttt acttatttct atttgtgtcg atattaattg
    27721 cgataaacct atagccagtt tttcatgggt aaaggatatc ccgtttcttt ttgcaaagta
    27781 taatttaagt gtaccgataa tttcatcctt ttctttcaga ggcactataa gcccggactt
    27841 cagctgacag tcttccgaag aacactcaat ttcttccggt gaattcatta tacgcatctg
    27901 accattcatt attacttgct ttgtagcaaa ggtttgtata ggatcaccgc tgatatgatg
    27961 atccgaaccc aatcccacat gtgccaggat tgtagttttg tcagtaattg aaaccgcagc
    28021 agcgcctgta gattccctta ttattgtaca tacttttttt aaagaatcac cgttgatttc
    28081 cctgaaatac gggagggttt tattcgctat ttcgagagcc aattgagctt gcattgcagc
    28141 catttgctct ttttctttga agctattctg aatcagtaat atgaggatac atatacctat
    28201 agcatttgta aaactcattg ggacataaat gctttttaca atggcaagtg cactggaaaa
    28261 aggctttgac atgagcagaa tcaacagcat ttcaatactt tctataatga tgcccccggc
    28321 aagcccataa agccatttct gatatcggat tcctttctta tagatcaagg cggaaaggaa
    28381 accggcaagg attgttgata ttgcacaggg aacggaggtt attccgccga tatcatataa
    28441 aaatctgtgc aagccggcaa taatacccga tacaataccc acaaagggac cgcagagtat
    28501 gcctccggtc attacgccga taattcgcgt attagctatc gcacctaata cgtctgttcc
    28561 tgtatatgta cttataatac caaatacggc aaaaattaca gataatatca gaaggtctga
    28621 ttttttaaat gcatcctttt gtattatttt cttgaatacg gaaaggtttg aaacaataaa
    28681 agctactata attacataaa ttaaactgtt tatcaggttc ttaataagat caatcatttg
    28741 atacttctta atctcctttt catattttta aaaaaagcaa aataaggact atcaatgaga
    28801 tattcctttt ataacatttt acctttttat tctgaacttt gcaaatagtt ttggggcaag
    28861 gtatcccttg ccccaatgct ttaccaaacc tccggaaaag cagcgccaac atataaatct
    28921 tccaatttag tcttatgtcc ttgttccatt gctgcaagtc caaggaatgt ttccttttgt
    28981 tctttatcgg tacttatagc tgcaaatcct ttatacatat ccattgcttc ttcttccctt
    29041 ttcattgcca gggcaatagc atcagcaggc ttcatatcaa tggaaagctt aggcttgtct
    29101 actgattccg caaccttgta gtctgcagtc gagtcgaact ctaacttctt tccggtatct
    29161 ttaaaataat cttgaagtat gactttatgc gataattcgt cttttgccag gttctcaaat
    29221 atttcagaaa tgttcttgtc ctttgtcttc tttgcaacat cccgatagaa ggaataagct
    29281 tcctcttcgt tttttattgc atccgatatt atttttttgt attcttcaag agtcatttta
    29341 caaccctccc tttgttaaca ttataacata attatacata ataaatagtc ttttttaaag
    29401 gctgaataac taatgctgat ttgctttggt gcaggatacc gggtgtaaaa ccatacaaaa
    29461 actccagtat tttatacaac ttacctaaaa atgataagat gcatcaatat actggagcct
    29521 gagcgaacca ttttgaaata tattacttct tcaattcctg ttttccggtg actttgcttt
    29581 tatcatcaat tttgaaagtg gatttggcat catctgttgt aaaatataca ttcccgtcga
    29641 tggttccatc aaccagctca aagttctttg cagaaacata aacatcaccc ttaaaggttc
    29701 ccccctggat tctggcattt gggcttttga cagtaagctt tggtgctgtt agtgtgtatc
    29761 ttgctgttac gtttttgtct gcatcctgcg cgtaaagagc tattttacgc ttgagatcct
    29821 tagccggatc gttcttgtca tggaattcgc cttccaatat aagatcctcg ttaattgaca
    29881 aatcctttgt agtgcatatt atccaggtgc cgtttccgct tatagctttt ttaaaggcat
    29941 cggcatcagc tactattgaa gcagttgtta caacatccgg cgttgcctcc tgcggcgttg
    30001 cctccggcgg tgttgtctgt ggtggggtct caggggctgc aggctccgga gcacagcctg
    30061 caaataatgc aataataacc gcaaatacta atactatgac ttttcctttc atcggaaatc
    30121 ctccttgcat ttatatatac tttaaattac attattgcct gaatgataga atttaatgta
    30181 tcactgcagg ccggatacat gtcaacaaaa aatattaatt ttgaaaggaa ctgccaacaa
    30241 tatatttagc attaagattc tttgtcaaaa aagaggaaaa tttgattgca tgtcgaataa
    30301 gatagtggat ataggaccat aggtctatat atattcaata aataggacca gagaactata
    30361 tattgattgg attattacaa gattttgcag gagtggatta tatgcaggaa gcagcaggtg
    30421 cttttaaatt atatatcgca aagaataatg cagttatgat acaggctgct gataatcaaa
    30481 aagcagtgtt ttattttttt attatacttc tgatagcggc tgctgttgct attatacatt
    30541 attacgcagc cagaaatcgg agagggactg tgaacagcaa aaaagaaggc gtaattagct
    30601 atcggacaat aattcaactt atgcaggagg gatttgcatt atttgagata atatacgacc
    30661 agggcgggaa gccaatagat tattatttta ttgattcaaa tgaggcagct gaggttatta
    30721 gcggaattga aaggaaaacg tcaattaata aatcggtttt tgatattctg cctgattcgg
    30781 ctgcagaatt ggcaaaaaaa ttgaacgaag tcacatttgc aggagaacca tataaggatg
    30841 aaatatatgt caaggaatgg ggaaagtatt tatcagtaaa catgtataag gctgaaaaaa
    30901 aactgcttgc agttatgttt tcggatatta ctcaaaagaa gctggcggat gaggccatgc
    30961 agaagcagaa gctcagctat gaggcattgt ttagaaattc ttccgatgcc attatccggt
    31021 ttgaccagaa tcataacata attgacataa atgagaaatt tacggaattg ttcggatata
    31081 cccttgatga aatatatggc agggagttag acagcgtatt agctccgaaa gaaaagttcg
    31141 atgaggcaaa agtattgaca aagaacgtgc tcaacgggaa tattaccgct atggaggaat
    31201 cagtacgttt aggatctgac ggaaagacca gagaagtaag tataaaaggc gttgcaattg
    31261 ttctaaacga taaaatcatc ggcggctatg gtatatatac aggtatatcc cgaaggaaaa
    31321 aagcagaaag agaattaatg tatatgaact atcatgacct gctgaccggt ctttataaca
    31381 gaaggttctt cgaggaggaa ttgaaacgcc tggatactga aagaaatctg ccgatttcca
    31441 ttattatggc ggatgttaac ggattgaagc tgtttaatga tgccttcggc catgctgaag
    31501 gtgataagct attggttaaa gcagctgaaa taatcagtaa ggaattgcgg gaagacgaga
    31561 tagtggcaag gctgggagga gacgagctgg ttattttatt gccagggact gattcggagg
    31621 aagcacgcag catagtcaaa agaataagag cttccatttc aaacatacgg ctgaaatcca
    31681 tagaactttc aatatccttc ggatgggata caaaaaacag tgccgatcag gatatgaatg
    31741 atatattgaa aaatgcagaa gattatatgt acaggaataa attatttgaa agtcccaata
    31801 tcagagagaa gaggataaag gcaataatta attcctttta tgagaataac agtgaagaag
    31861 aacagcactc aaagcgggta agctccttgt gcgaggccac cggtgccgct atgggcatgg
    31921 ccggagctgc cctgaaggag cttaggacat taggcttgct tcatgatatc gggaaaattg
    31981 caattgataa tagtatactg aataaaccgg accggcttac aaaagaagaa tggaatgaga
    32041 ttaagcgcca tccggagata ggatatcgga tactaagctc agttaatgat atggcagaat
    32101 tggcggtatt catactcgca catcatgaac gatgggatgg aaaaggttat ccgaagggaa
    32161 taaagggaac ggacataccg ctgcaggcaa gaatcacagc agtagcagac gcttatgatg
    32221 ctatgacaag taaaagtgca taccgaaatc ccttgtctga aagcattgcg gtggatgagc
    32281 ttgaaaaaaa tgcaggcaca cagtttgatc ctgaaatagt gcaaattttt attgataagg
    32341 taataaataa aaaagtcaaa acttctgttg acattgacgt tacgtaaagg tgtatagtaa
    32401 tcttatcggg ctcaaggcgg tgatgatatg gaatacacgg tgcagaagct gggaaaatta
    32461 gcaggtgtca gcacgagaac cctgagatat tatgatgaga ttggaattct taagccggcc
    32521 agaatcaatg catcaggata tcgaatatat gggcagaagg aagttgacag gcttcagcag
    32581 attctcttct acagagagct gggagtggga ctggacagca taagggaaat cgtgacggcg
    32641 ccttcttttg acggagctaa agcactgcgg gaacatcgcg ggaagctgct tgagaaaaga
    32701 gagcagttgg atatgctgat tgctaatgta gaaaaaacca tagcgttaac agaagggaga
    32761 attagtatga ccgataaaga aaaattcgaa ggctttaagc aaaagctgct ggatgataat
    32821 gagaaaaagt acggtaagga agttcgtgaa aaatacggtg agagtgcagt agaaaaatct
    32881 tatgataaag taaagaacat gaccagggaa gagtatgatg agctgacaaa gctgtctgag
    32941 gaaataacaa aaatcttggg cgaggcattc aaaaccggtg acccggcagg agatttggca
    33001 cagagggctg cggatttgca caagcagtgg ctcacattct actgggacag ctattcaaag
    33061 gaggctcacg cggggctggc acagatgtat gtggatgacg agaggttcag agcttactat
    33121 gacaaaaaac agcccggcac ggcagaattc ctgagagatg caattctcat atataccgga
    33181 atgaaaaagt agaagcaata accttggagg acacggggag aggacacggg gacgtttcga
    33241 ggacacgggg acgtgtcctc tccccgtgtc ttttgttaca gttttattaa aaagcaaaga
    33301 agcagaaggg catttgcata aacttgtaga aataccataa agagcataaa tactacaaaa
    33361 ttcatttatt aaggagcaaa ttatgaagtg tatttcaaag ttcatcattt ttctgtttgc
    33421 ccttttatta acattatctg catttccagt ttccgcagct tcggaggccc cggagaagga
    33481 ggaactcagg ggagtatggg ttgcaacagt ggcgaacata gattatcctt caaagtccac
    33541 tgccgattca gcgatattga aaagtgaagc cttgaaaata ctggatgatg ctgaggccat
    33601 gggcttgaat gcgatttttc tgcaggtcag gcctgcttca gatgcccttt ataagtcaaa
    33661 gcttttcccc tggtcaaagt atctgacggg gagacaggga ctcgcacctg ataatggctt
    33721 tgatcctctg gagttctggg cagcagaggc gcacaagcgc ggaatggaac tgcatgcatg
    33781 gatcaaccct tacagaatca caaaaaagac tccaaaggag ccaaagcatg accttgcttc
    33841 tcttgatcca tctaacccag ccagattgaa gcccgaatgg gtggtgaagc atactgacgg
    33901 aaatctttat tacaatcccg gaattcccga ggtcagaaag ctggtaatag acggggtgct
    33961 tgaaattatc gaaaattacg gtatcgatgg tatacatttg gatgattatt tctatcccgg
    34021 aaaggacttt agtgataagg cagcttatgc aaaatatgga acagcttatg gcaatataga
    34081 tgactggcga agagaaaatg taaacaagct tatcagcgag ctttcgaagg ccgtaaaggc
    34141 ggccgatgaa gctgtaagct ttggcgtcag ccccttcggt atttgggcaa ataaaagtgc
    34201 aaatacgctg ggaagtgata cgagaggcat gcaaacctac tatgaccaat atgctgatac
    34261 aaggaaatgg gtaaaggagg gcttgctgga ttacatagcg ccgcagctgt attggtatat
    34321 aggctatgaa attgcagatt acagcaggct gttgagttgg tgggccgatg taactgcggg
    34381 tacgggtgtt gatctgtata tcggacaggc agcatataga accggggata cagatcctaa
    34441 aagcccatgg tatggtattg cagagatgga aaagcagctt aaactgaatt ctgaaaacaa
    34501 ggaaataaaa ggaagtatct ttttcagcaa caggtcttta tgcaataacc cggctctatg
    34561 tgcggctttg caatctttat ataaaaggaa gagcagtatt ccgatcagtg tttcacgtcc
    34621 ggctgaagat atacagacca ccttggaaaa atattacctg aacggttcat ctgatccgga
    34681 aaagcctttg tatctgaacg gagtacctgt ggaaaacagg tcggcccggg gatacttcgg
    34741 agttttggta cccttgacgg agggtgcaaa ttcttttaca ctatcccagg aagcctcaga
    34801 ggtgacccgt gtgatataca gaaaaaccgc tgattccgaa ccggcaaaaa tgagcggggc
    34861 tgagataatt tcagcttcag tctttccgca aagccaggag taccgtaatc ccggggaaaa
    34921 aataactctt tcctgcgaag ctccttatgg ctcaacggta acggtgcaga taggcgggaa
    34981 aagatattta atgaaatctt caggcgcagt gacaggaacc tcagggctgt atgcagacaa
    35041 atttacctgc gaatatacta ttccggcata taagggcact ccccgtaata tagacctggg
    35101 gaaaccggtt tatacaatga attacaaggg tacggccaag agctgtaccg cccccgcaaa
    35161 gatcggtgtg ataatgaaag gttcaccatt ctatgcaaag gttgaaaaag aggtcatata
    35221 tacatacaat gaggcaagct cttcagatgg agcagcctat gagttgtaca gcggaatggt
    35281 tgattctgtt accggaatgt cggggggcta tgtccgtctg tccctggggc aatgggtcaa
    35341 ggacagtgat gttgcggtat atacttccaa ggcgcaatca aggccgatta taaaggatac
    35401 tgagtatata tccggggatg ggtgggattc tttaaaactt gatatatcgg ctccgcctgc
    35461 cgctattgca gattttgacg gagccctgct gaaaattaat gtatcagccg attctcaggc
    35521 agcatctcct gaactgccgg atgattcacc tttttcatct gttgctgttg caaaaaaagt
    35581 gaacaatacg gaatatacca tggcattgaa aaaaaaccgg agcataagcg gctattatat
    35641 gcaaaagaca aaaacaggac tggtgctgaa tataaaaagg cctgtaaaat caaataatag
    35701 cgatgagcct ctttccggcc tgacaatcat gcttgatccc ggccatgggg gcagtgatac
    35761 aggtgcaata ggccctctcg gaacagagta tccggaaaag accataaatc tgaatattgc
    35821 atttaaatta cagtcggagc ttgagcagct tggggcaagg gtatttctga caagagcggc
    35881 agatgtgaat gtgtccctgg aagagcgcct tgcagcctcc agaaatgcaa agcctgatat
    35941 gttcctttct cttcattcca atagcatggc ggaggatgtt gatatatcca aagtagacgg
    36001 atttgcagtt ttctaccggg aaaaacttgc actgccattg gcagaaacaa tattccaaaa
    36061 tacattgaca catctgaaca gaaccaacaa agggctgcat aacaagaatt tttatgtttt
    36121 aaggggtacc tggacaccat ctatccttgt tgaaagcggt tttgtcccga acccatatga
    36181 atttgaatgg ctgattgatg agaatgagca ggccgggctt gcccagagca tagcgaaatc
    36241 tgtagttgaa tatttcaagt aagtaatgaa catactcttt tttttgataa tttgatagaa
    36301 taagaatata gctgaagcag gaggcaggcc catgtcggaa ctcagaaaaa tgaaagcaat
    36361 agtattatta agtatcgcaa ttatgacggc tatagtgatt ttacccgcaa gtgccgcagt
    36421 tggacagcag cctccttttt ctgaagtcaa ggaaaagctt tcgggaattt ctaaagagga
    36481 aaaggagatt ttgcagaaac tgttcatact tgctcaggag attgaaatag cggaggcgga
    36541 ggcagaggag ctatcccgcg aaacagaagc aatcagtaaa gaaataaaag gcttggaaga
    36601 ggatatagca aaagaggagc ttgcttatga aaagaagcgg aagagcttgg agcaggttct
    36661 gaaaagctat cagaggatgg gagccgggtc attcctggaa atcatcctgg actcggacag
    36721 cctgagtact tttttgcata ggttgaacat actccgggat cttaccaaaa ataccggaac
    36781 gcttttggag cagctggagt caagcggcag gaagcttgca gaggaaaaat caaaactatc
    36841 ggaaaagctt gcattggtta aagataagga aagtatgtca agaacggcgt tagctgataa
    36901 aaacaggctt aaggcggaaa aggaggcata cctggcatcc cttaagggag agaaggagca
    36961 ttaccaggaa tacctttcca atctggaaag ggtctggaat gagcttaagc ctttgctttc
    37021 agagaccgca aaggaattct cccgaatgat tgaagaggag ggattgcctt atgatgcttt
    37081 aaaaatcagc ttttctcttt ttgaagtcaa gggagctatt gacgatggga ctataaacaa
    37141 aattatttca gagaatgcca atcttgaaga aatgattttt gcttttcatc aggaagcggc
    37201 tgaaataagg atacctgata agaaccttat attgacagga acttttgcaa ttcaggaagg
    37261 acacactctt aagtttcagg cccgggaagg aagctttttc ggattgcctt tggaaaaggg
    37321 agttctggag gaattattcg gagaggaagg catgatgctg aatcttgagc cgctgctggc
    37381 aggaaacgat atacatgccg taagaattaa agaagggtac atagaactga ttcatagatt
    37441 ttaatttgtt attgtatttg gaggggaaat aatgatagaa gctaagggag tatgtttgga
    37501 gtatccggat ggaaccaaag ccattaaaaa tattgagctt tcagttaaac cgggtgagct
    37561 tgtatacata accggaccca gcggctccgg aaagacaagc ctgctgaagc ttttcatggg
    37621 tgaggaatat ccgactgccg gttccctgag ggtgctggga cagcacatat ccaggggaca
    37681 agcgtcaaga atcagaggcc tgcgcagaat gatcggtccg gtttttcagg acttcaggct
    37741 gataaaaggg agaactgcct tggaaaatgt catgctggga atgagatttc tggatatacc
    37801 ttccggctgc ataagggata gtgccggaaa tgcactgaaa aaggtgggtt tggagcaaaa
    37861 aatgctttca ctggtagagc acttgtcctg gggcgaatgc cagagggtgg caattgcccg
    37921 ggcagtatca agagaaccgg tcttgataat agcagatgag cctacgggca atcttgataa
    37981 ggacaatgcc ctaaacatac tggaattgct gaaatccttc agaaacgaca ataaagccat
    38041 aatcatcact acccatgcaa cccatttgat tgaagatgaa agatatgcag tacatatccg
    38101 gatggataac gggaacatgc tcaaagaaag gtcaggggaa gcttagatga agaatatttt
    38161 gtacaatacc ggttatttca ttaaagaaac aaagacaata atgcgtttga acctgctgtc
    38221 aagtatttta tcttttctaa gcatcgggct tatttttttc atcctcgcca tgatgatttc
    38281 cggctggtgg gtaagcagcc atgttgtgct tgccatacaa gaagaggctg aaataagtat
    38341 atatttcagt gaggatatgg acgctgccgg tgcagaaagg ctggccgaag agattgaaaa
    38401 cattgaggga gtatatgaag caaggctggt cagcgaagag gaagcctaca gcaggatgga
    38461 aaaaatactg gggaaggaag cccgtgttct tgagtattta gatgataatc ctttcagtcc
    38521 ctttatagaa gcaaaaatcc aaatagaaga tataggcctg ataccgggga aactagatct
    38581 gattgagggc attgagcatg tgagggataa cagggaagtg ctggaacgca tcctcggaat
    38641 ttcacagaca ataaagatat tgggatacct gattatagcc gctgtcggga tatccacaac
    38701 ggttataata tcccacatta tcagaatggg aatatacgac tgcagagaac agataaatac
    38761 cttaaggctg atgggagcgc ctgaagcttt tatcgggttt cccttcatgc tggaagggct
    38821 tcttctcact ctgggaggag gcgcgttatc ctcagccttg gcagcatatg cattaaagta
    38881 tttgtatgca caggcagccg gcccgatagc cttcattcct cttccgcccc gggaggcatt
    38941 ggtttctgac cttactatat tgattatgtt attgagtacc gcattgggaa cagccggaag
    39001 ttttttcggt ttgtcatcgg caaaaaactg aacaagaaaa cgcacatggg cggatatcat
    39061 aatctttgca tatttatggt ataattaatg cataacaaag gagggaatgc aaatgagttt
    39121 taaaatgacc ggcaaagtta cctgggtagg gaaaattgac tgggagttga ggagatttca
    39181 cggggacgag tattcaactc acagaggttc ttcttacaac tcatatctta taaaggacaa
    39241 gaagaatgta cttattgaca cagcttgggc accctttgca aaagagtatg tggaaaccct
    39301 aaaaaaggaa atagacctgg ataaaattga ctacataatt gcaaatcaca gtgaaattga
    39361 ccacagcggg gcactgccgc tgctgcttgc ggaaatacca aatactcccg tttattgcac
    39421 agctaatggg gccaagatat taaaagggca ttatcataag gactggaatt ttgtaacggt
    39481 aaaaaccgga gatacccttg atattggtga aagcaagctt acttttgtgg aagccaggat
    39541 gcttcactgg ccggacagta tgttcgtgta catgtcaggt gaaaacatat tgttcagcaa
    39601 tgacgccttc ggacagcatt atgcctcgga gctgatgtac aatgatatgg tagacaatgc
    39661 tgaattatat caggaagcaa ttaaatacta tgcaaacata ttgaccccct tcagtacctt
    39721 tgtcaccaag aaaatagaag aagttttaag cttcaattta ccggtgaaca taattgcccc
    39781 cagccacggg ataatctgga gggacaatcc ggcccagata attcagaaat acatggattg
    39841 ggcaaaggac tatcaggaaa atcaaattgt aatagtatat gatacaatgt ggaacggtac
    39901 aaggaggatg gccgaagcta tagcggaagg aatcagatcg gttgataagg aggttgcagt
    39961 aaagctgttt aatgctgctc attccgataa aaacgatgtg ataacggaaa tatttaaatc
    40021 caaggcagta ttgttcggtt cttccactgt aaataagagt attatgcatg cagtgggcgg
    40081 gatgttggat atggtaaaag gcttgggctt caagaataaa aaagcagcag ccttcggctc
    40141 atacggctgg agcggggaat cggtcggtat attgacggaa agattgaagg atgccaaatt
    40201 tgaaatagta agcgacggaa taaaagaact gtggaatccg gaccaggatg caatgaacag
    40261 atgcgtgcag ttcggagcgg attttgcagg gaagctgtag tccggaactt ttcgcaaaga
    40321 tgctgcgtca taactgtgta cggaaaaata tgcagtgtct ttgaagggag agttatgcta
    40381 ataaaaatgt ttaataaaaa agcttttttc aggtctgctt cattagttat gtccattgtt
    40441 gtgcttctgg gtgcaggatt gctgacaggg tgcacggcac ctgcagagga taatacaggc
    40501 ggcaaagctg aggataataa ccctcctgct gtggaggaaa gcattgagga taggattatt
    40561 cccgagttta tggcattggt gggggggaat cccaagcctg atgcaataat tgagttcatg
    40621 gataagagta ttgctgacgt ttccggagaa aatgcgtcta aaatgcttga tgaacttgaa
    40681 aaatccctga aaagcaattt accggagctg gaggagaaat attacagcac ggcggtacaa
    40741 gaagcaatgt tcaatgcgta caaatcggaa tttgatctaa gcaaacttga cagcattcaa
    40801 gatgctgaag ttaaaagcct tattgatgaa accaaggcta tgggctataa ggtggaaaca
    40861 gcggaaggca tgtttttccc tattattaac tatgaatttc ttaagaaatt cagttcttat
    40921 gcagaggatg acatgaagga ttatattgat ataatggcag cagagagcaa taaagttccc
    40981 gcaaaggatg gggctcttgt gataggctgg gatgaggtaa ttgagagggc gttggcccag
    41041 gagaacttta tggcgaaaca cagcagctct gccaaggttg gaagcataaa ggaactgcag
    41101 aaaaaatata tcatgttcat tctgtacgga ttgaataata cccctctatt cagctatgat
    41161 acaaaaatga tgaatcctga agccaaagat gcttatacaa aggttttgaa ggaaaaagga
    41221 gatagtgagc tgttgcagct tttgggcgga tatttggaaa tcttagagaa atccgattac
    41281 aagctgactg aggaagcgga taagtataga aaagatgccg aaggacagct gtaataattt
    41341 ttaaaaaact cctaatgtga ataattgaca aatgtaaaat tcttcgttat actatctttg
    41401 gtataacgaa gaattttatt attggtttga ctagagtgca gattgaaagg aataccggaa
    41461 tggagaattt agaatcaata gagcttcttc aaaaaaagca cggaaatggt ataaccaaac
    41521 ctgcgagaat agtcatttac cttatgcttc tgctgataat tgtattcatt tcatcttttt
    41581 gttttggaag atataatgtg aacctgcatg aattactgag aatacttgct aaccgggtta
    41641 ttcccctcga acatacctgg acagcagaaa tggaaacggt agtgataaat ataaggctgc
    41701 cgagaattgt tctggcctgt cttgtaggtt gctgtctatc ggttgcaggg gcatcatatc
    41761 aaggggtttt tcaaaatccc atggcatccc ccgactttct gggagctacc tccggcgctg
    41821 catttggagc tgctctggcg attcttttgg gcagttcaag cagaatgatc actatattgg
    41881 cttttttctt tagtttggtt tcggtattcc ttgtatattt cataggaagg tatgcacccg
    41941 gtaagggtgt gattaacatg attcttgcag gtattatgat aagctcactt tttacggcag
    42001 gtacatcatt tatcaagctt gtggctgacc ctaataatca gctgccggct attacatatt
    42061 ggttaatggg aagcctctca ggtgcgaaaa acaatgaagt ggtttttgca ttaatcccta
    42121 tggctatagg tatgattccg ctatttgtta tgagatggcg cataaatata cttacactca
    42181 gtgatgagga atccaatgca ataggtatca atgccaatat gctgcgcctt attgtaatca
    42241 tttcttccac cttgataacc gctgccagtg tctcagtcag cgggacaatc ggatgggtgg
    42301 gtcttgtgat acctcacttg tgccgcagga tgatagggaa taactacaca tatctgctgc
    42361 cggcgagcat ggctttcggt gcaaccttta tgctgctcgt ggacaacatt tcacgtaatc
    42421 tgctggcaac ggaaattccc ataggtatcc ttactgcatt tataggtgcg cccttcttta
    42481 tccgtctgat tttcagaagg gaggggaccc tgtgattatt gaagtggaaa aactatgctt
    42541 tggatatagc tcccataagg tgctcaagga tatatccttt aatgcgggaa agggaaagct
    42601 gatttccata attggtccca acggtgtcgg caagagtacg ctttttcgat gtgtcctggg
    42661 acttattagt gaatacagcg gaagtattag gattgagggt aatgatataa aaaagctttc
    42721 tgtgaagagt cgggcgcatt taatggcata tatacctcag acctactatc catcctttaa
    42781 ctattccgta atagacatgg tacttatggg cactgcgcat cagctttcgg cgtattccat
    42841 gccggggaaa agggaaacgg agcaggcatg gaaggcactt gaacagttga atattaccgg
    42901 tcttgcccaa agaggctttt gtaaattaag cggaggggag cagcagcttg tattgattgc
    42961 acgtgcctta gcccagcaag cgggaatcat tcttatggat gaaccgacat ccaacctgga
    43021 ttttgggaat caattaaggg tattacagca gataaagaac ctgacaaaag aaggctatac
    43081 agttttgctt tcaatgcaca atccacagca tgccctgacc tattcggatt atatactggc
    43141 actttttgac ggcagcatta taggagatgg aacgcctcat gaagtgataa atgaggattt
    43201 gctcagtaga ttgtacaatg ttgaggtgca aattgctgaa gtaggcgggg acatgttaat
    43261 cgtgccctcc gggaaaggca gggttgattg tgtttaaatg ggatttacgc atgatacaat
    43321 tcatgaagga tgcaatagag gagaatgata tatatttcaa gtctttagcc ggtaaggtgt
    43381 cagaatacct gcctgctgat gcccatgtat gcgatgcagg ctgtggactg ggctatctta
    43441 gcactgaact gtcaaaatac tgcagaaatg ttactgcggt tgacattgct tcactggcat
    43501 tggttgagct gagaaataag ttaaatcaca tcaactgcaa taatatcaaa attgtagagg
    43561 gagatataag agtgaatcca cccggtatac cttatgacgc catggtattc tgcttgttct
    43621 ccagtttgga ggatgcattg aagatatcta aagaacagtg cagaggcaca actgtcatga
    43681 tcaaaagaaa ctggaataat catttgtttt ctatatcgca aaaagcattg gaacggcata
    43741 ctctgcagga atcattggag caccttaaga tgctaaggat accttacaaa tccgaaaatt
    43801 ttattttgga aatgggtcaa cccttcagat caatggcaga tgccgtagaa tttttccaaa
    43861 tgtacagcaa tgacgattac ccacatgaga taaaagctga ggatattgca gataggctgg
    43921 tctaccgaga atctgaccgg tttccctatt acctgccggc tttaaggcaa ctgggtatta
    43981 tagtggtgaa aacttctgat attccggata atatagaaat aatatggagg aggaatgagg
    44041 aatgaaaagg attattgcga taactatagc cattgtcatg atacttacaa tgcttgcagg
    44101 ctgtaaggca ccggagcctg catcagcccc ggctgaagca gaagccatcg ctcaatccgg
    44161 cagtgacaat gctactgtag ttttcacaga ttcagtggga cgggaagtag aggttcccgc
    44221 aaatattgaa aaagtggcaa tctcaggacc ccttgcgcaa atagtggtat ttgcacttgc
    44281 accggataag ctggtaggaa tcacggcacc atgggataaa agcgcggagg aatttataga
    44341 tacgcagtat tacgacttgc cgatactggg tcagctatat gggggaaaag gcgaattgaa
    44401 tcttgagacc ttgctggcca gcggggcaca ggtgatcatt gatgtgggtg aacccaagaa
    44461 aactattgtt gaggatatgg atgagctgca gaagcagacc ggaattccct ttgttcatat
    44521 aactgctaag actgaaacca tggatgaagc atatcaaatc ctcggcaagc ttcttaatat
    44581 ggaagtacag gctcaggttc tgtctgaata ttgtaagcag acctataaac gtacccggga
    44641 aattgcaggc agcgtaaata aggtaaattt gctttattgc ctgggagaca aaggattgaa
    44701 tgtaattgcc aaggattcat atcattcaga ggtaatagat cttttgtcca ataacatagc
    44761 tgccatggac aatccctctt caaagggcac aggcaatgaa gtggatatgg agcagatatt
    44821 gaactggaat ccggaggtaa taatatttgc acccaatagc atttatggaa cagcaggtac
    44881 ggaccctgta tggcagaaga taaatgcaat aaaggaaaac cgctattatg aggtgcctaa
    44941 cagtccgtat aactggttgg gtttcccgcc ttcagtacag cgctatctag gaatgatgtg
    45001 gatgtcacag ctgctttatc ctgaaacggc gaaatatgat ttgtacaaag aggttcaaaa
    45061 ctacttcaag attttctatc attgtgacat ttccatggag cagtatgaaa aattagttgt
    45121 caactctata ggaaaatagg aaggctgaaa tatgagcaga ccgcagcata ttctgatttg
    45181 cggagaaaga ggagtgggta aaagtacttt agtgaacaaa cttcttttgc aaagccgtcg
    45241 tcctgtattt ggttttatta caaagaaaat gcctccgaac cagaacggag aatgtgatgt
    45301 gtacatccat ccggctaatt ccgcaaactt acagcttacc catgaaaatc ttgtaggtat
    45361 ctgcaataat ggtaaaatag taactattaa tactgaagtt tttaatacag taggcgtgaa
    45421 gctgctgcag tctccaccta ggggattgct gcttatggat gagttgggct tcatggagag
    45481 caaggcatca gctttctgct caggtgtgct tgctgtactg gatggagata tacctgtgct
    45541 ggcagcagta aaaaataagg aaacaccttt tctgaaagct gtgcgtgagc ataagaatgt
    45601 tgagctccat tatatcacaa ctcagaacag ggatatgttg tatcaagagc tgattgagaa
    45661 gattatggaa tacaatcagt ggaagtgaac cacagagaac tttagcttga aagagcatat
    45721 agcgtatgtt aatataatag taattgaata gcttaagtac tgggggggct ggtaaggccg
    45781 gctgagatag agaacatgag tctctgaccc tattacctga tctggataat gccagcgtag
    45841 ggaagtaaca tgtttataga atataagcat aaatccttat aatggggttt atgctttttt
    45901 atcgtataga aaattataat ttcctatacg atgggggatg caagggggag aagccccctg
    45961 ccgataaagg aggatttttt atgcaaaccc aaaaacttac attttctgca ttgcttgtag
    46021 caataggcac aatatcggcc aatttggtct acatacccgt gggagcagcc aaatgcttcc
    46081 cggtgcagca tacaataaat gtactgtctg ctgtgctgtt gggcccgtgg tatggcgtgg
    46141 ccaatgcatt tctgatttcg ctgctgagaa acatattggg caccggttcc cttttggctt
    46201 tccccgggag catgattgga gcttttatag cagggattat gtatttgaaa ttcggcagga
    46261 agttatttgc cgcagccgga gaaatattcg gtacgggcat attgggcgga cttttggctt
    46321 ttcccatagc aaaatttttg atggggaatg atgcggccgc cttttacttc gtagtacctt
    46381 tcctgataag cactattggc ggcagtataa ttggctatgt aattctggga gtaatggaaa
    46441 aaagccatgt actgaagaaa acaggcattt agcgggagga attgatatga aaaacgtttt
    46501 gaccatagca ggctcggaca gctgcggggg cgcaggaatc caggcggacc tgaaaacctt
    46561 ttccgcccat ggggtttacg ggatgagtgt tattactgcc ataaccgcgc agaacacaca
    46621 aggggtcctg gcagttcagg atatttcagt tgaattgata gagaagcaaa tagaagcaat
    46681 ctttgaggat attagggttg acggtttaaa aatcggaatg gtctcacaga tagacacgat
    46741 aaatgccata ggggaagaac tgcgttacta taagccttca aatattgtac tggatccggt
    46801 tatggtttcc aaaagcgggt atcatctatt gcagcagtcg gcaaaggaag cattgatcaa
    46861 aagtttggtg cccctggcaa caattgttac gcctaacatt cccgaagcgg aagtgcttac
    46921 cggaatgaga atcggaagta ttgaagaaat ggaaaaagca gcaagggtga tttttgaaat
    46981 gggtccctgt aacgtacttt taaagggagg ccatcttgat gaggatgcaa cggatattct
    47041 ttttgacggc agtaaaatta gctgcctgaa aggcaagagg attaacacta agaatactca
    47101 cggaacaggc tgcacccttt cggcggctat tgcctccaac ctggcaaaag ggtattctgt
    47161 tgcagaagct gttgcattgg caaaggagta cattacaatt gcaatcgaaa attccctgtc
    47221 cataggcaag ggggtagggc ctacaaatca tttttacacg ttatacaaga aagcgggtat
    47281 gctggatgaa taaatcaaac aataatctta cgctattaag cttcgccgct ctctggttcg
    47341 gggcagccgt atcaattgct gagatactga ccggaggatt gcttgccccc cttggcttta
    47401 agaatggtat aattgcaata ataatcggac atataatagg cactttgatt ttagtggcag
    47461 gggggataat cggaacagaa acagggctgc cttcaatagc gtcaacagga atatccttcg
    47521 gcaaatacgg agcttatgtt ttttcggtac tgaatgtgct tcagctcctt gggtggactg
    47581 cagtcatgat aatatcaggg gcaagatcgg tcaatgaaat tgcaaaagta ttgtggtcct
    47641 ttgacaatat ttatctctgg agtgtcgtaa taggcgggct gatttgtttg tggatatttt
    47701 ttggaaatgt cggtgtgaaa aagcttaacc tgattgccgt agtgctgctt tttatactta
    47761 cagtaatgct gagtgcggtt gtttttaaga atagtgaatt attcagcagc tcgggggacg
    47821 gcaccctatc cttcgggtct gccatagagc tgaatgtgat aatgccttta tcatggcttc
    47881 ccctgatagc agattataca aggtttgcaa aaagaaaagg agatgctgtt acaggaagct
    47941 ttctgggata ttttacggga agtacatgga tgtacacaat tggtcttggt gctgctatcg
    48001 catcgggaaa ttcagatccg ggggcaatga tgctatcggc aaacttaggg ttcactgccc
    48061 ttggcattgt gatactgtca actgtaacga ctacctttct tgatgcaaat tcggcaggag
    48121 tgacattttt gaatttattc ccaaagctga acgaaagaaa gactgcatta ggcatgggag
    48181 cagcagggac tttgattgca gtaatagtac cgatagaaca atatcagaat ttcctgtatg
    48241 caatagggtc ggtttttgca ccgctttttg caatattgct tacggaatac tttgttttaa
    48301 agaaaagcag gaagcttcag gaggggctgc tagtaaactg gggagcgtca gcagtatggg
    48361 ttgcgggtgt aattatgtat taccagttca tcaagcttga ttttgtaatt ggggcgacag
    48421 ttcccgtaat gataatcacc ggattgttat attgcttgtc atggaggtgg acagagagtt
    48481 ggatattatt ggagaagcat cggaactgct gataaggata aaagaataca ggcctttaat
    48541 tcatcatatt accaactatg ttactatgaa tgattgtgcc aatgccgtac ttgcgatcgg
    48601 gggctctccc ataatggcaa aggacccctc agaggttgaa gaggtggcgg caatgtcaag
    48661 agcattggtg ctcaatatgg ggacgccgga caataataat ttcagatcaa tgctgctggc
    48721 aggcaaaaag gcaaaagaaa tgaggattcc tgtaattttt gatccggttg gtgccggagc
    48781 tacttctttc cgcagagaga tctgctgtaa gctgctgtcg gatatcaagc ctgatgtaat
    48841 tagagggaac atttcggaaa ttatgtattt ggcaggcaaa gaccgttatt cttgcggagt
    48901 agactccaat tgggatattg ataatgctgt ggggatagtt cagggcttgg cgaaggattt
    48961 aaattgcgtt gttgctgcca caggaaggaa agacctggtt tcagacggaa gtacggctta
    49021 tataataaat aacggacatg agatgctttc aaaaatcacc ggagcgggat gcatgacttc
    49081 atccatcgtc ggcactttct gcagtatgcc ggaggactat ttgacggggg ctgtgggggg
    49141 cgtaatggtt atgggaattt caggacagct ggcaaatgca tgtttaatgc ccaatgaagg
    49201 cataggaatg tttaaggtaa ggctttttga cagtatgtat aatttgaaca gggatatatt
    49261 tatgagagaa ggtgtggtat atgccggata aagggttggt gaattattct ttatatctga
    49321 taaccgacag aaaagtactt ggagggaagg aactgcttga ctgcattgag gatgcaataa
    49381 agggcggagt gacattagtg cagcttaggg aaaagaatat aaatactttt gattattata
    49441 acctggcttt aaaggttaaa gagctggtaa ggaaatacgg cattcctctt ataattaacg
    49501 atagggttga tatagcattg gcggtggatt cggagggagt gcatctgggc cctgaggatt
    49561 tgcccatagc ggctgccaga aaactaatgg ggcgcgataa gataataggt gcctctgcca
    49621 attgcattga agaagcactt gattttcaga gacaaggagc agactatctg gggattggtg
    49681 cattgttccc gaccaaaaca aaaggcaata ccgagcatgt aacactgacc caattgaagg
    49741 caataaagga agcagtacat atacccatag tgggcattgg tgggataagc actgaaaacg
    49801 ccgcttcagt caaagcagcg ggagctgacg gaatagcggc ggcctcagga atcctgggaa
    49861 gtgagaatat agttgaagct gctcgccgcc ttaaggggtt ataatatttt ctttgaaata
    49921 tgtcgaatat accgttataa tgaatataca atcaaataaa agatagaact gttgcacaat
    49981 atatgaagaa tacgacgggg acacggggac gcggggaaac gtccccatgt ccccgcgtcc
    50041 ccatgtcccc gtcgcattat tcttcatatg cgaaaaggag gatttctatg tggaaagagt
    50101 ttaaggcatt tgcaatgaaa gggaatgtaa tagatttggc ggttggtgtt ataatcggcg
    50161 gagccttcgg gaaaatagtc tcttcattgg tcaatgacat aataatgcct tttgttggct
    50221 tgctgattgg aaagctggat tttgccaatt tattcataac cctgggcgac ggaagcttca
    50281 acaccctggc ggaagccaag gaagcgggtg tcgctacctt gaattacgga ttattcatca
    50341 acaatatcat tgactttctg atagttgctt tttcaatatt tttagtgata aagcaaataa
    50401 accgtattcc aaggaagaag gaagaacctg cccctgttca aacaaagtct tgcaagtact
    50461 gtttcagtga aatacatatt gatgcaagta aatgtcccca ctgtacttca gatttgaatg
    50521 actgattcat attagtatag gaacagcatg tagataatac tctgcatgct gtttcttttt
    50581 tatcagctga tttaattgca ccagcgataa aggttatcat aatttataaa taatattaat
    50641 cgataaacat tgataatgtt tatacaatat gatagaatga agtagtaata aatatttaac
    50701 aaacaatttg gaggaggaaa catgaaaaaa gtattatcaa tcgttttatt actgacatta
    50761 gcactttcag tactggccgg atgttcagca cctgcagcac cggcagaacc agcggcaccc
    50821 gcagcagaag gcggcagtat tgttaaatta ggcattggac atattactac aattgccaag
    50881 tcaaaggatc ttgggaaaga tgctgaaggc aaagaagttc ttcctttagg acaggttgat
    50941 acagttatgg ctgctgttgg cttcgacaag gatggaaaag ttgtttcagt gactattgac
    51001 aatgcacaga ctaaggttac gtttgatgcg gctcttcagg ttacatcaga tataaaggct
    51061 gaaagcaaga caaaggtaga gcttaaagaa gaatatggaa tgattaaggc ttcatcgata
    51121 ggcaaagaat ggtatcagca ggctgaagcc cttggacaat ggatggttgg caagactgtt
    51181 gatgaaataa aggctatgaa gacaaagaac aaggatgcat cacatcctgc agttcctgat
    51241 gttccggagc ttacatcatc tgttaccgta tctgtacagg aatacattgc agctgttgcg
    51301 gaagcttatg aaactgctgt cgatgtagca ggcggtgaaa aggttggact tggacatgtt
    51361 ataacaacag gtaaatcaaa ggggcttgga aaagatgcag aaggcaaaga agtccttccc
    51421 ttcgcccagg ttgatacagt catggcggct actgcctttg acaaagacgg caaggttgtt
    51481 ggaactatca tagataatgc acaaactaag attacttttg acaataccgg taaagtaact
    51541 gcagataaag cagctgaata tccgacaaag gtagagctaa aagaaggcta tggaatgatc
    51601 aaggcttcat cgataggcaa agaatggtat cagcaggctg atgcccttgg acagtggatg
    51661 gttggcaaga ctgttgacga aatcaaggct atgaagacaa agaacaagga tgcatcacat
    51721 cctgcagttc ctgatgttcc ggagcttaca tcatctgtta ccgtatctgt acaggaatat
    51781 attgcagcag ttgcggaagg ttatgcaaac gcaaaataag gcgtataaat attaagggac
    51841 acggggacga agggacacgg ggacgtttat cttgtcccgc tttcagcggg acaagataaa
    51901 cgtccccgtg tcccttcgtc cccgtgtccc ttcacggatt ttttgcataa aacatggatt
    51961 atttgctata atagctttgc taataaaatt aagggtgata agaatgaaga aaacttccgt
    52021 tattgtaatt acattggtta tactgctttt ggttttatca ggatgcggga aagctccgga
    52081 attagtatta actaaataca gtgacacctt tttcggaact tttgatacac tgactcaggt
    52141 agtgggctat aacaaaagcg agcaggaatt ttcggagtat tttgagaaga tacgcgctcg
    52201 ttttgaagag ctgcataagc tgtatgacat atataatgat tacgaaggta tcaataatat
    52261 caagacaata aatgaaaatg cgggaatcaa acctgtaaag gtggataagg aaataacaga
    52321 tctcatattg tttgcgaagg aatggcatgc aaaaacaggc gggcgaacca atattgcaat
    52381 gggtgcggtg ctttcaatat ggcatgatta tcgcgagcgg ggagagggcg atcccgtgaa
    52441 tgccgagctg cctcctttgg aaaagcttag agaagcaagc aatcatactg acataaataa
    52501 agtaatagtg gatgaagcaa atagtacggt atttctgtct gataaggaaa tgcgccttga
    52561 tgtcggagcc atagcaaaag gctttgccac ggagctggtt gccaaggaaa ttatgtcgga
    52621 gggttttact tcgggcatca taagcccagg gggtaatatc cgcgtacttg gaaagcctct
    52681 tgacggaatg cgtgagtact ggggtatagg tatacaggat ccggacaaaa tgtttatagc
    52741 tgcagaagag aacctgttgg atacgatatt tgtcaataat gcagcaatag taagcagcgg
    52801 ggattatcaa aggtattatg tggttgaagg caagatctat catcatttga ttgatccgga
    52861 tacgctaatg cccggggaat actaccgtgc agttacgata gttactgagg atgcgggact
    52921 tgcggatttt ttgtccacta ctgcatttct gctgccttat gacaaaagcc gtaaacttgt
    52981 ggaaagcctt gacaacgttg aggcggtgtg ggttatgccc gatgggaagg ttgaagctac
    53041 ggatgggatg aaaaagttaa tgaaaagcca tggtgcaacc ggttcaaaag ctgaataaca
    53101 tataaatgta acaattgcct cccgcaatga atattatggt attgttcact agctttgcag
    53161 gaggcgtttt tatggaagta ttgacattgg gctcaagagg tgcagcagta aagcttatac
    53221 aaagcctatt gataagaatc ggatacaacc cggggccggt ggacggtatc tttggaccgg
    53281 ttacccgtga agcggtaagg gaatttaagt tggataacgg acttgaaccg gacggggtgg
    53341 caggcccggc tgcctggagc aggtttgaga gattcctgag gggttatgac acctatacta
    53401 tccgaccggg ggatacgttt tacaatatat ccggaaggta ttacactaca ttgaatgctg
    53461 taatgacagc caacccggga gtcgatcccg ggaacctcag agtcgggcag gtaatcaatg
    53521 tgccttatgg cattgatgtg gtttttaccg atatcgatta tacatatgag ataatggata
    53581 gagacataag agggctaaag gtgagatacc cctttataga agtgggtatc gcgggaagaa
    53641 gcgtccttgg gaaaaactta tactatataa aattgggaaa cgggccttca gaagtgttct
    53701 ataacggtgc acaccatgca cttgaatgga ttacggctcc gctgcttatg aaatttattg
    53761 aaaactacgc ccggggatat gcaaggaatt ccggtatcca gggctataat atacgtgacc
    53821 tgtggaacag aggcagcatt tacattatgc ctatggtaaa tccggatgga gtaaacctag
    53881 tgttggaggg gttgaaaaga gataatccct attatgaccg gctgcttgca tggaatgata
    53941 cgggcctgcc cttctcacag gtttggaatg ccaatatcag aggagtcgat ttgaaccgca
    54001 attatccggc gtcatgggag gaagcaaagg cacaggagcc ttcgctgggt gtagacggcc
    54061 cggggcctac cagatatgga gggccgagcc ctctttcaga gcctgagaca caggctgtcg
    54121 tcaattttac aagacagcac aattttaagc tggttatagc ctatcatacc caaggaaggg
    54181 taatatattg gctgtataag gggattaggc ctccccgggc attggagata gcaaatattt
    54241 tctcggaaat aacaggatat gcagtttcgg gcgtgcctta cgaagcagct tacgcaggct
    54301 acaaggattg gtttgtggag cagtacagaa ggcccgggta tacttttgag gtgggaatcg
    54361 ggcggaatcc catacccatt tcacaatttg atacaatata tagggagaat gaagaggtac
    54421 tgctgctggc accgatagtg taaaaatgac gggtaacact aaacacacgg ctgttccacg
    54481 aaataacacg gtactagata gttattaatc gatatatcaa tagatctcga tacgtttgct
    54541 attgtcgatc attactaaca tacctgaata tttttttgcc gtgatttctg tgggttttcg
    54601 tgccttcagt gttatcggta tctttatata cattcttcaa ataatgtggt ttattgacga
    54661 tattctatat aaagcgtggt gattgcatga gcagcggtaa aaaagaattt tttgagaagg
    54721 atttgtacaa gcctatttat gactatcttg ccggcttggg ctatacagtg cgcagcgagg
    54781 tcaaccactg cgatattgct gccataaaag agaatgaact gcttgtagtg gagatgaaaa
    54841 aagctcttaa cctcgatgtc attcttcagg ctgttcagcg acaaaagctt gcagacatgg
    54901 tttatattgc tgtgcctaaa ccgggcaggg ggcttttctc caagagatgg cagaatctgt
    54961 gttatatgtt gaaaagactg cagcttggcc ttatggttgt ttcgcttaag gagggctgtt
    55021 cctttgttga agtcgtgttt gagcctgcgg cctttgatat ggctaagagc aagaatcata
    55081 gcaaaaggaa aaggcaggag ttagtaaagg agtttgccgc aagatatgga gattttaaca
    55141 ccggagggag cacaagggaa aagcttgtga ctgcatatag ggaaatggca atacatatag
    55201 cctgctgtct gatgagatac ggtcaattaa agcccaggga actgagacag cttggcaccg
    55261 acgagaaaaa gacaacagcc atacttcaaa agaatcacta tgggtggttt gaaaacatat
    55321 ccaaaggctt gtacgcaata aacgataaag gaaggcagga agtatcggaa tatacggagt
    55381 tgtatcaata ctatatgcac cttgcagaag agaaaagcga gagtgaggat aaataaagtg
    55441 aataaaacta caaaagcttt gatatccttg ggagtcaatt gcctgcttgt tataatcgca
    55501 tattttgttt ccgtatggct taaagctgct ccgccaacca taaagctgct ttcgcagtat
    55561 gtttcctatg tactgtttgc tgcagctatg tttttcgggt ggtggttcaa caggagcagg
    55621 gtgttttttt gctccctgac gctgatgctg tgccaactgg ctctctttca gttaatccct
    55681 caagagccgg atgccgccga atatttttat atgatactgt caatgacatc agtgtatata
    55741 ccccttaata ttctggtttt ctcaattcaa agagagaggg gcatagcgtc atcctgggga
    55801 aggatgaggc tgttgtttat tgctgcggag cttctaatcg gcgcttggat aatatggtac
    55861 aacgattcgg acttacttca gcttttcaat attaagctgt ttccggccga cataagtttt
    55921 attccggatc tttctcatat gtcactgctg ctgtatttta ttacttttat atttcttttt
    55981 ggcagacagg ctgttacggg gaaaaatcat gacacacctt ttatgggtat aacattattt
    56041 tcctttgccg cactgatgtt taataaggac ttaaataccg catcagtgat gtttgcacta
    56101 tccgggctaa tgctgtcagc ggtggtgatc cgggcatccc acagcatggc ttatttggat
    56161 gaacttacag gactgccctc aaaaagggct ttgaaggagg aaatgcttaa gcttggaagc
    56221 aaatatacca tagctatgct tgatattgat tattttaaga aattcaatga tacatacggc
    56281 catgatgtgg gagacgatgt gctcaagctt gtggcggctt gcattaaagg tgtcggaggc
    56341 ggagggaaag ccttcagata tggaggggag gagtttacaa tagtttttcc cggcaagact
    56401 gcggaggaag caataccata tctggaagag ctgcgtgaat atattgcaaa cagaggtttc
    56461 ataaggagaa gtaaaagcag gccaaaagaa aagcctgctg atatcgtgac aagaagcagt
    56521 tcatataaaa agctttattt gacggtaagt atcggagcgg cggagaaaaa tgaaaaatac
    56581 agaacgtcgg aagaggttat taaggcagca gacaaagcat tgtacaatgc taaatcgaaa
    56641 ggaagaaact gtgtgtgtaa atcttgacca ggacatgggg acgtttggga cacggggacg
    56701 tttctcccgt cc
//
LOCUS       DAOQUR010000012        53986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_679_length_53986_cov_11.553819, whole genome shotgun sequence.
ACCESSION   DAOQUR010000012 DAOQUR010000000
VERSION     DAOQUR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 53986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53986
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_53986_cov_11.553819"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1141
                     /locus_tag="PKI50_04185"
                     /pseudo
     CDS             134..1141
                     /locus_tag="PKI50_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1321..2097
                     /locus_tag="PKI50_04190"
     CDS             1321..2097
                     /locus_tag="PKI50_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02946.1"
                     /translation="MDGATGLREANFTSDKTDARWFVKAWSEYLKKALENSNDSLRYI
                     VKNGIGSVKELYLNEVNRENIDKIDLPSASAGIYRIGEGIFEYLLIGDICPVIEDIEG
                     NIKEIKDTKLEVLDNIAVNELRRLMASRGLSFNEAREGIQEILLKHRRLKNTDKGYWT
                     LEFEPSAVDYCIYDKLPLESISKALFMTDGFFAIHSSYNYFSVKDLISFTDMKGLEKV
                     YKTLRDIEEEDKECNRYPRFKKSDDASAIYIKYEDVQAVY"
     gene            2184..3413
                     /locus_tag="PKI50_04195"
     CDS             2184..3413
                     /locus_tag="PKI50_04195"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT02947.1"
                     /translation="MSYFDSYRGVPRSVYIIFFAQIINRFGDFVLPFLSLFLVKKLGL
                     SYQDAGIAVMLTSLSTIPGSFAGGKIADHLGRKKSYAFFQLSAAVCLFFCAFSENPQF
                     IIALICMASFSNGAVRPIISAIITDVLPAEKRKMGFSLSYMGINLGVSLGPLVAGFLF
                     NRYIPLIFIGDAVTSLIAVILVLTNIKETLPDYSDNKCLNEEEKSEEGNVFSVLIRRP
                     KILIFLTINIVFNVVYAQHSFSLPMMLDNIFGSSGASNFGVIMSCNALTVIMLTMLIA
                     HATSKWKPLSAITIAGLLYAIGFGMITFISSMPLFIISTVIWTTGEIMTVTNFGVYIA
                     NNTPQNFRARFNAISSLSWAVGAALGTSVMGRYMDFFGVKAVWPLAFCLGLAGAAGMY
                     LLESHSNFRVSRAKELN"
     gene            3458..4819
                     /locus_tag="PKI50_04200"
     CDS             3458..4819
                     /locus_tag="PKI50_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT02948.1"
                     /translation="MNGDMTQGSSLKALITFSVPLMLENILQQLYYITDAVILGRFVG
                     KNALAAVGVANPIMSTAIFFMFGVCIGTAIFMSQIFGAAKYEILKREVSTALIAGAVF
                     TGLLSIISVLSARYILIAIGTPEEIMNDAEIYLKILSGGLIFSFLYNFYSFTLKSIGD
                     SKTPFIFLSISCVLNGILCYCFVALLGLGVVGSAAATIIAQVVSSVMCISYVYIKVPM
                     ISLKPNELVMDKSLLRKTIKYSSITALQQISLYMGKLLVQGVVNPFGTNTIAAYNTVT
                     RIDAFVLSPGDNFVVSVSTYTAQNMGARKWKRIIEGYKIGNIIITIYNLAVAAIVFFG
                     AEKLMHLFISASEREVIMIGVEYLRLMPLFYVLTGFCNIFQGIFRGIGKLHITFFATV
                     TQVSARVILALIFAPYFGISGICYAVGIGWVIMIIYEGLIYRKEYLRMKAFDTAQDVL
                     DSA"
     gene            4842..5939
                     /locus_tag="PKI50_04205"
     CDS             4842..5939
                     /locus_tag="PKI50_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HNT02949.1"
                     /translation="MIDYEGQYPVYEISASKIKSNARKIYEMCRANGIEPAGVVKGFS
                     AIPEVAELFIEAGFKTLGDSRMKEIIKLRRAGIKGELMLIRIPMPCEIPELVSFADSS
                     LNSEMETIRLINKEAGLQGKVHGVILMVDLGDLREGFFNREEAIEAAKEVEMLENVKL
                     LGIGTNLGCYGSIKPSEKNLRELCRLAELIEKEIGRSLEIISGGASTSLSLLVNGKMP
                     ERINHLRIGEAIITAKDFPYVWGIEMPGIYRDPMVLKAQVVEVKTKPSYPIGEIFVDA
                     FGNRPVYTDRGMRKRAVLAVGNKDVGDFSKLKPRHEGIEIIGSSSDHLILDIEDYAGD
                     IKVGDVLEFDTFYAAMVFLCSSDCVRKAVVE"
     gene            complement(6027..6206)
                     /locus_tag="PKI50_04210"
     CDS             complement(6027..6206)
                     /locus_tag="PKI50_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-type small acid-soluble spore protein"
                     /protein_id="HNT02950.1"
                     /translation="MDQRRAKAITASPVMTHVTYNGIPIYIENVDEETGIADIHPLDQ
                     PSNKLEVPVTSLVEH"
     gene            6636..6833
                     /locus_tag="PKI50_04215"
     CDS             6636..6833
                     /locus_tag="PKI50_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNT02951.1"
                     /translation="MVGTVKWFNGEKGYGFITGEDGTDVFAHFSQINAEGYKSLQEGQ
                     KVSYDVTNGPKGPQAENITII"
     gene            7022..7612
                     /locus_tag="PKI50_04220"
     CDS             7022..7612
                     /locus_tag="PKI50_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT02952.1"
                     /translation="MKVKGLIITACIVFALASFLPGCSVSSSNNTNINQEKEEPKVLT
                     IYSGRQEKFIIPLVEKFEEDTGIKVNLISGKATEYAHRIIEEKENTQADVFLSNDGEI
                     LEYLRIENMLSPINSKLLEEVPGNYVGKDNTWTALTIRYRVFIYNTDLITLDEMPDSI
                     FDLTDPKYKGQFTMNRAGNESMITYFASIQLKLKLL"
     gene            7670..7957
                     /locus_tag="PKI50_04225"
     CDS             7670..7957
                     /locus_tag="PKI50_04225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNT02953.1"
                     /translation="MIKVVAKSFAKPDKLDKILKLSAEMVEKTVKEEGCIKYELFQDI
                     KAPEVLVIVEEWESEEALSRHMASEHFRRIVPQLNELREKASEVNILKKLF"
     gene            complement(8006..8422)
                     /locus_tag="PKI50_04230"
     CDS             complement(8006..8422)
                     /locus_tag="PKI50_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNT02954.1"
                     /translation="MEVNTSLTQNIDALFTNIETFTQNEGLLGKPVSQGDKTLIPIIS
                     VTIGYGGGNSSGKMSGGAAGSNQGTGNAGMDALGLGARISTDALALVDGENVSILPVN
                     ANPGNVNQLINQLPQIMGKGQNNQQQNQQQNSQQNS"
     gene            complement(8451..8930)
                     /locus_tag="PKI50_04235"
     CDS             complement(8451..8930)
                     /locus_tag="PKI50_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02955.1"
                     /translation="MTLLFILLLSLLLFIFLLFTVASRIDLSYDSARSDMHLTLRWLH
                     PLLRSVIVKEGNGFLLLVYLLKKRVLAKLMKPKQYTKRSGNILKSLKPTEIHVNTQYG
                     FRDPFVTGLACGAVSAVSQLLNVESLHQKPDFLANSDYINLDATAKLNLGRLLMKLI"
     gene            complement(9176..9634)
                     /locus_tag="PKI50_04240"
     CDS             complement(9176..9634)
                     /locus_tag="PKI50_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNT02956.1"
                     /translation="MSIIIGDNAPDFSLKDNKEQIIRLSDFRGKKVLLSWHPLAWTSV
                     CTDQMRSLEVNWEKFHSLNTVPLGLSVDPVPSKKAWSAVIAIDNVSLPSDFWPHGQVA
                     RDYGIFIEKKGISERANIIIDENGIVRWIKVYPISQLPDINEVLEVLSGI"
     gene            9919..11268
                     /locus_tag="PKI50_04245"
     CDS             9919..11268
                     /locus_tag="PKI50_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT02957.1"
                     /translation="MEEYKNFVITIVLIFSLLIISVIMLAAYINRISKIKRELQIKNE
                     ELTASDDKLKKQLEEHERVQHSLITSEKRYLLLFEKMMNAFFIFEPVIDEEGIISDIR
                     FLKVNPGFELHTGLKGAEIIGKKWMEVYNYPNKNLHIYHRILLTGKPEHFETYYEQED
                     KYYLTNGFKISDNEVGAIFENITRYKKAIKEITMLNEELEHKVAERTDELQNALNELE
                     AFTYTVSHDLKSPLRAIEGYSKFVIEDLESGPGEETAEMIHNIRNISLDMIEMINKLL
                     EYSTMTKTEIAKESVDSGELFKSIINELLSVNTDRNIELKAVTELPVVFADKVMFKQV
                     LYNILSNAVKFTKLKDRAIIEVGCSINEGEYIFHVRDNGVGFDMQYSGKLFGIFQRLH
                     TKDEFDGSGIGLVTVKKIIQKHGGRVWIKGSMGQGATVYFTLPVERGGIYAAGNDCG"
     gene            11243..12373
                     /locus_tag="PKI50_04250"
     CDS             11243..12373
                     /locus_tag="PKI50_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT02958.1"
                     /translation="MLQVMIVDDVDIVRKELKRLKLWGDKTGFVISGEACNGHEALEK
                     LEESKFDMVITDIRMPKVDGIELLKEITGRKLCPCVVLLSDYSEFVYARQGLVLGAFD
                     YIIKPVAEEELEKLLQRAKEYISEKRLEEQRILQLEQKLVEKVDMFFPQSEVSQLIDA
                     IMAGHTKTIENAGSIFEIVCTNMNCDLIKVKSLLNSIMLEITNKISASCKWLDKYTDI
                     NAIRTIDFSGANDLNSIKNRFVSAVGRISGLFARLKHGTEDNGITGQVCRYVLENIDG
                     NITLSAVSESLFMSKSYISEVFKQKTGLSFIEYLTIVKMERAKKLVGDGRLKTYEVAE
                     LMGFKDIEYFSRLFKKYTGITPTEYRQGMEGEKYKDPKKFGV"
     gene            12460..12825
                     /locus_tag="PKI50_04255"
     CDS             12460..12825
                     /locus_tag="PKI50_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT02959.1"
                     /translation="MKQIDVLVVEDSFYSADLSIRVLKKAGFNVRYKIASSSITMREA
                     LKDRHWDLILSDNYMPGFDALQAIEIRDQSGSLAPFVIVSEYIQEIDIRKAMEKGCCA
                     YIAKEDLDKLAQHIEKILE"
     gene            12874..14532
                     /locus_tag="PKI50_04260"
     CDS             12874..14532
                     /locus_tag="PKI50_04260"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNT02960.1"
                     /translation="MVFNKNEKKLERIEKVTFGNEYREFLGKGELESNQISLRIALTY
                     MIVVALWILLSDKILGLLAADNAVITRISMIKGWVYVLISGILIFVIVSYSLKRVKIA
                     EGKLYDSCQNLSEANMELEHLAYHDQITGVRNKISLIKRINELVSSDDSKKIAILIAD
                     IDNFKYINDAMGHSIGDQVLIKFSSRLKGLAGVDSTVYRLGGDGFVIALEGYEEASSV
                     KRLAESLLKDFKTPLDVNGSKLFITISIGVSMYPENGDGMDELLKNADIALNKAKATG
                     KNRVVFYNRTMNEAVSERVNIEKHLRAALINNEFELYYQPQWDIVQNKVSGLEALIRW
                     KNPELGYVNPLKFINIAEDTHMIIPIGEWVLKSACTTLKELHRQGHEGLSVSVNISMV
                     QLLQDDFVCKVTGILDYAGLSPEYLELEITESVLMESCDALEGKLKILKDMGIKIALD
                     DFGKGYSSLNYLKQLPISTLKIDKSFIDTIESDSKHKSLTNVIVKLGRIMGLSVVAEG
                     VETKEQLDYLTRHRCDKVQGYLFSKPVPGEKVIEAIRGLNVSFR"
     gene            14865..16439
                     /gene="gpmI"
                     /locus_tag="PKI50_04265"
     CDS             14865..16439
                     /gene="gpmI"
                     /locus_tag="PKI50_04265"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNT02961.1"
                     /translation="MSKRPVVLVVMDGIGINEEEKGNAVKAANTPTLDMLMNHSPYTL
                     IKAHGTAVGLPSDEDMGNSEVGHNALGSGQIFSQGAKLVNESIENGKMYSSDTWRKIA
                     GNCVANGSALHFIGLLSDGNVHSNIAHLKSMIIRAKEDKIKKVRVHALLDGRDVLATS
                     ALDYVEQMEKLFKELNDGSFDGRIASGGGRMKITMDRYEANWDMVRLGWDTHVSGKGR
                     QFGSASEAICIYREELPGVIDQDLPPFVIAENGIPAGTINDGDSVVLFNFRGDRALEL
                     SMAFDYEDFDKFERGRYPKVIFCGMLQYDGDLKLPKNFLVSPPNIKNTLSEHLVKHGI
                     KQYAVSETQKYGHVTYFWNGNRIEKFDDKLEVFEEIPSDRVSFDERPWMKAAEVTDKL
                     IDALKSGKYDFLRANYPNGDMVGHTGSFNATRIAVEAVDLCLARVIKAADEARAILII
                     TADHGNADEMLEKTKGGTVKAKTSHTLNPVPFIIHDKVQRHEIKEGSFGLANITATIT
                     TLLGIDHPDCWEESIV"
     gene            complement(16934..18124)
                     /locus_tag="PKI50_04270"
     CDS             complement(16934..18124)
                     /locus_tag="PKI50_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNT02962.1"
                     /translation="MERITCTISRGLRAPIIRQGDNISDIVVNTVLKASESEGFSIND
                     RDIVAVTESVVARAQGNFASIDDIAEDIKSRFGSSTIGVIFPIMSRNRFAICLKGIAK
                     GAGKAVLMFSYPTDEVGNQLIDIDTFSEKNLNPWSDVLTEGDFRRYFGYSRHTFTGVD
                     YIEYYKSIINEQGIECEIIFSNNPLTILKHTKNVLCCDIHTRARTKRILKENGGEKVY
                     GLDDIMSSPVGGSGFNEVYGLLGSNKSTESSVKLFPRNCQPIVEKIQAMLKEKTGRTV
                     EVMIYGDGAFKDPVCKIWELADPVVSPAYTSGLEGTPNEIKLKYIADNNFAHLKGEEL
                     KKAVSEYIRCKGSNLVGCMEAQGTTPRKLTDLIGSLCDLTSGSGDKGTPIVYIQGYFD
                     NYVK"
     gene            18417..18764
                     /locus_tag="PKI50_04275"
     CDS             18417..18764
                     /locus_tag="PKI50_04275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT02963.1"
                     /translation="MIEKRRHKRLPLKLELKISSLFRQDYEIIDDINEGIEIRNISKT
                     GIGFFCTHELPLNYYFDAKIQLTPDSHFYAVVKIIRMEKLEEGYFAGCEFIGLADILS
                     MRVDMYEDELNNI"
     gene            complement(18803..19201)
                     /locus_tag="PKI50_04280"
     CDS             complement(18803..19201)
                     /locus_tag="PKI50_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018671081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HNT02964.1"
                     /translation="MMPMVTFGSKKEKNYVQKTESSYEYKFIKETQTHNHEFLGSTML
                     PSQDVEAHNHRFAGVTSEVIFVPGGHVHTFMTDTDFFFGHIHGVGGTTGLQVPVDGGR
                     HVHFASDTTAFDACHDHDFQFATLIENPLE"
     gene            complement(19388..19579)
                     /locus_tag="PKI50_04285"
     CDS             complement(19388..19579)
                     /locus_tag="PKI50_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscE/YscE/SsaE family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HNT02965.1"
                     /translation="MSGKNAKQQVQEVFSQLNNAKTSLNQALNSAEKPENKQQIQNTL
                     NAIENAINTTSTALSNYKD"
     gene            19754..20506
                     /locus_tag="PKI50_04290"
     CDS             19754..20506
                     /locus_tag="PKI50_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNT02966.1"
                     /translation="MKLISWNVNGLRACIQKGFLEYFREADADVFCIQESKLQEGQVD
                     LKIPGYHQYWNYAEKKGYSGVAVFSKAEPLNFTCGIGREEHDKEGRVITLEYNDMYIV
                     TVYTPNSQEELARLGYRMQWEDEFRSYIKGLDGKKPVVVCGDMNVAHKEIDLKNPGSN
                     RRNAGFTDEERNKFSEFLEAGFIDTYRYFYPDRAGVYSWWSYRFNARANNAGWRIDYF
                     CVSERLAERLISADIHSQVLGSDHCPVELIMK"
     gene            20670..21848
                     /locus_tag="PKI50_04295"
     CDS             20670..21848
                     /locus_tag="PKI50_04295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT02967.1"
                     /translation="MIKTFDELGMDERLIEGLKKQGITEPTPIQADTVKLALENKDIV
                     GQSPTGSGKTLAFLLPIFQRVDAGKRENQAIVLEPTHELVMQIEKQARLLAANSGIAV
                     TSAAIIGEVNITRQIEKLREKPNIIIGSAGRVLELIKRRKINAQTVRTIVIDEGDRML
                     DEHNLGTVREVIKTTLRDRQLMVFSATIGEKALSTAREFMKDPQVVRIEDRGKISPDI
                     RHIYLAAEKRDKIEVLRKLAASMKPERAIVFINKSEELQLTAAKLKYHHLSACDISGS
                     SSKEERKKALEGFRKGDIKLLVASDIAARGLDIKGVTHIFNLDLPEDPKGYLHRAGRT
                     GRMGEPGTVVSIVTEREVPFLRKCERDLKIKIYEKIIARGKVLDPPQSKTSAGKGAGR
                     "
     gene            22189..24117
                     /locus_tag="PKI50_04300"
     CDS             22189..24117
                     /locus_tag="PKI50_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT02968.1"
                     /translation="MIKIGIIVPNDLVNIAKSAAEGIEDEITILYGSMTEGISLAKQL
                     EECNYDIIITRGGTQVLLTQSNINIPVISIPITPIDVYEAVNEAEKISKDISFIVSQN
                     MISIIESYIKISGRNFKVFQIKEESEIEKKIRELATEGKKVVVGLGTIAKYASSYGLV
                     PIVIKSGKESFLSTIMEAKRIVNATKKEKKDKERIKAIIEHTIEGIICVDKKGHIIII
                     NEVARKLLKCDNNGLVGKMISSVLPDLQLEDTLYNGIMETEVIKNLKDTKVMVYKIPI
                     IVDDEIENAVAVLRDIDEIQKADEKIRQDIAVSGHFAKYTFNDVIGCSESIREIVRIG
                     KEYAKVNSTVLIEGETGTGKEIIAQSIHNYSNRSNKPFVAVNCAALSESLLESELFGY
                     SSGAFTGADRKGKRGLFEQAHTGTIFLDEISEINPLLQGRLLRVLQEKQVMRIGDNKL
                     IPVDVRVISATNKDLHNLVVAKKFRDDLYYRLNVLRIELIPLRYRREDIPCLIDYFLR
                     AYCAKLGKENIVFTSEAAEHLSKYDWPGNTREVRNFCERLAVMAKKTIITLEDVTNHI
                     INIGVNYNNADELVDIVGKNKNLSKDNLNLNNMTKQSIELALIHSNGSITNAAKELGV
                     SRTTIWRKMKKYNLLVKK"
     gene            24314..25474
                     /locus_tag="PKI50_04305"
     CDS             24314..25474
                     /locus_tag="PKI50_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNT02969.1"
                     /translation="MERRTYLQIPGPTNIPDRILRSLSQPLINHRGPEFGELLTKCVE
                     GLKKVYKTKNDILIFPSSGSGVIESAIVNLFSPGDTILTASIGVFSERAATIAEKFGL
                     NVIRITKEWGESVKPEDVKKVLEEDTDKKIKGVFVPHNETTAAVASDIKGISEVIKEI
                     NHPAVLIIDAVSSLACLPIETDDWAIDIVLTGSQKGLMLPPGMGIVSISPKAWELVEK
                     STLPKWYWSYKAVKERMEIKEFPYTPATTLLFGLSEALDIIEEEGLENVIARHELLAT
                     AVREAAKAMGLSIFTEEGYESNSVTAIIMPEGISYKDLAGILKTKFGVVVGGGLQKLK
                     GKILRIGHLGSIHKADIYAIMCAVEAALFELGYKVELGSAARAVSKVFLNDK"
     gene            25541..26620
                     /locus_tag="PKI50_04310"
     CDS             25541..26620
                     /locus_tag="PKI50_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNT02970.1"
                     /translation="MYYTTKEIEHKFVTDVFMHLGVKEEHAKMAADVVVQSDITGVTT
                     HGLARMPMYVRRLLQKTVNPTPNIRQTNDSENLIALDGDNGPGNVVGPTAINMCIEAA
                     KKHGIAAVSIKYSNHYGVGNYYAWRFAEANLIGITMTNSTPSMAPTGGKTPMLGTNPI
                     TIAVPAGKRYPICLDMATSVVAKGKLLKAVEANSKIPFEWAVDKEGRPTDDPNEALEG
                     SLLAIGGYKGYGLAMIIEILSAVISQSAYGLDIGDKFDADCTDQEKIGHFMLAIDVSK
                     FLDLDTFKKSMDQYIETMKNTEKAAGVEEIYVPGELEFLRAEKIKETGIPVPEGIQAK
                     MLKIAKDVGIGSDIKTIDDLFDRYK"
     gene            26693..27733
                     /locus_tag="PKI50_04315"
     CDS             26693..27733
                     /locus_tag="PKI50_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNT02971.1"
                     /translation="MKQYIDLRSDTVTQPTQEMRDAIYNAIVGDDILGEDPTVKELEE
                     LAAKMFNKEEGLFVSSGTMANQLAVMTFTNRGEEVIVGDTSHIYNLEVGGLSALSQVQ
                     TRPIHVPDGIYDIDILEELIQPKGIQSPKTGLICIENTNNLNAGLVVPVDNINEVCNL
                     AKKKDIPVYMDGARIFNAAIALDMNVKEIVRNTDAVMFALSKGLAAPFGAVLVGSKEF
                     INKARWFKQRIGGGYRQAGFMAAPGVLALRTMISRISIDHDNAKLLGNGLSNIVGLRI
                     DTTRIHTNIVSGSIELLNITIEDFLNRLLSKGIKAKRISETSFRMVTHLGIDESHINY
                     VIESVKDIITNL"
     gene            28021..28350
                     /locus_tag="PKI50_04320"
     CDS             28021..28350
                     /locus_tag="PKI50_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HNT02972.1"
                     /translation="MSEDTILIGGYSNFPSGAAAKEVYGMLGVALEIDPKTGKIVNAA
                     TTLLTDVTKNFVSRILVGQNMNEGLKEPIKSIERRYFGRNKKALIAALMDAHRIYEKY
                     LEEKYNL"
     gene            28632..29723
                     /locus_tag="PKI50_04325"
     CDS             28632..29723
                     /locus_tag="PKI50_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNT02973.1"
                     /translation="MYAERHNKLLEVMRDKGWDCVILWPSPNMYYMTGYYPKPDERLQ
                     IAFIPVQGEPVLIVPRLYSGESKHKCWIKDQRSWIDGDDILALTKDVIKDLGLKDARI
                     ALDDTMGFLQVSTIQAACPKAEFGLAGTVMSPLRLEKGPEEIALMQKSSQISDEVMQI
                     GIDACLSGQNEQQLVDLVEYELRKRGMANGFSNLIASGTHSALPHHVSSKRVPQKGDA
                     VFLDIGGAYERYWSDITRTIHIGPPSAKFIKAYEAVKEAQQRASEAVKPGVTAEEVHE
                     TAFGYLEKKGLSKYFFHRVGHGMGLEGHELISINRGNHLVLKPGMTFSVEPGVYFEGE
                     FGIRIEDTVVVTETGHKSLTEFTRDLIVL"
     gene            29787..31367
                     /locus_tag="PKI50_04330"
     CDS             29787..31367
                     /locus_tag="PKI50_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT02974.1"
                     /translation="MKRMRYLALVMVLVMLLSVGCSSSGGEAPGAEAPSEAGSEASTP
                     AKTTLVYGVGVDATTMDPHFANNTPTGNVSFQVHENLTAFDDPKTMKPVGKLAKDWKI
                     SEDGLTWTFNLREGITFHDGAPFNAEAVKKNFDRILNPDLGAPRRSNFKMIDRLEVVD
                     EYTVNIITNVPYAALPQLLTCYNASILSPKATEEYGDQYSAHASGTGPFKLGEWIPGE
                     SMTLDRNDNYWGEKPSLEKLVFKIIPEDASRVMAVQTGEVDYISNVPPFEIENLKSSG
                     DVTVEMHEGFRTIYLGMVINRPPFDNIKVRQALNYAIDRDAIINDFLEGSATYAIGPE
                     ATSIPGASSDLFKYTYDPEKAKALLAEAGYPDGFEIRFVSPHGRYIMDRQIAEAIQAQ
                     LSKVGIKANLQILDFSLLLNMLPTGKDAELMLMGKGSPASDPDFDLTNYFTTDGTNNW
                     YFISDPKIDELTDKQRATMDLNERYEVLQELQEKVQEELPWASLHYERQIVALGKGVA
                     GVTVWPYEYIDFSKAKFE"
     gene            31451..32371
                     /locus_tag="PKI50_04335"
     CDS             31451..32371
                     /locus_tag="PKI50_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02975.1"
                     /translation="MFKYLKRRLLMLIPLLLVVSIIVFSMIHLMPGDPAMIIAGEGAS
                     QADIEAVKHKHGLDKPLFVQYFTWLGNIIRGDFGKSLRTGRPISQEIGFRFVNTIKLA
                     TLAIVIGSILGLVMGIVSATKRYSFFDNVTMVIAMVGVSITPFYLGLMSILLFSVKLG
                     WLPMMGVESWKGWILPATTLAFRPAAMIARMTRSNMLEILGQDYILSARALGLPNKVV
                     FVKYALRNALNTVVTVIGLQLGLLMGGAVMTETVFSLPGIGRLAVESIKGRDFPSIQV
                     SVLLIALSFVIINLAVDILYAIINPRISYN"
     gene            32394..33302
                     /locus_tag="PKI50_04340"
     CDS             32394..33302
                     /locus_tag="PKI50_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT02976.1"
                     /translation="MESNNNTASMPMNDAKMEREARIRELKRFWLQFSKRPVAVLGLV
                     IVLIFLVMCITAPLIAPFDPYESDTSKRLLKPGEEGHILGTDELGRDLFSRIIFGSRY
                     SLLIGVGSVGLGALIGIPIGLIAGYYEGKIGNVLMRIMDVFLAFPNILLALIIVSILG
                     PSLYNALIALSIWSIPSFARIARSEVLALKNNEFVEASKAMGSRDSWIVLWHIFPNLV
                     SQLIVWITLNISSAILTGAGLGFLGLGAQPPSPEWGVMLANGRQYIMRAHHLTTYPGL
                     AILFLALGLNLLGDGLRDTLDPRLKD"
     gene            33325..34287
                     /locus_tag="PKI50_04345"
     CDS             33325..34287
                     /locus_tag="PKI50_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT02977.1"
                     /translation="MHTTLKIKDLSVEYHTFDGIVKAVNNLNLEVKKGESIGLVGETG
                     AGKTTTALSILKLLPYAGHVVNGEIEFNGKSLLKASEQEMYRIRGNKISMVFQDPLTS
                     LNPVYNIGTQIRDVLTHHQSLNKEEAIEKAKEMIELVGIPSNRLNEYPHQFSGGMRQR
                     VGIALALACNPELLIADEPTTALDVTIQAQILALMTELKQKFQMSLIMITHNLGIVAE
                     VCDSVAVMYSGEIIEIGSVKKVFNNPMHYYTKGLFGSVPSPDVKISRLNPIKGRMPDP
                     KNLPKGCKFYPRCNAARPECLKMPELIKVEEDHFVACVAGKEGV"
     gene            34287..35237
                     /locus_tag="PKI50_04350"
     CDS             34287..35237
                     /locus_tag="PKI50_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT02978.1"
                     /translation="MSKTFIEIKNLKKYFNVKDGKKLHAVDDVSFNVDKGEIVGLVGE
                     SGCGKSTTGRVILRLLEATAGNVFLEGEDIIQANKNRLKQLRRRMQIVFQDPNSSLNP
                     RKSVRQIISEPLIVQGILKSKEMQEKVYTLMEETGVAFDLVGKYPHELDGGVRQRVGI
                     ARALALGPEFIVCDEPVSALDVSIQAQMLNLFQDLKEKKDLTYLFISHDLSVVRHISD
                     RILVMYVGRLVEEAEADDLFINPMHPYTKALLSAIPVPKIRKESNRILLKGDVPSPID
                     PPPGCRFAKRCWMAKEECFEKEPELKEYSPGHRAACHFVE"
     gene            35265..36023
                     /locus_tag="PKI50_04355"
     CDS             35265..36023
                     /locus_tag="PKI50_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNT02979.1"
                     /translation="MTENKAYILPEMTWVEAEKAYKEAKIAIIPVGSHEQHGPGIKMK
                     VDGVQAYELAKLVAQGVHPLAVVTPPVNIGISHHHMKYPGTVTLKSSTFMAVIEDIVT
                     SLYRFGFKKFLIINAHGGNQYACNLATNDLKEKLGVEIAYTLYLDLVPELVERVPPEK
                     RGHGADPGLAPALYLDEDLVYMDRLEKGKEHWPYKMIGREKVKVNFPYYTHEISDNGV
                     WGDPEGTSKEFGKEICDAVVERLGVFIRDFVKNA"
     gene            36038..36817
                     /locus_tag="PKI50_04360"
     CDS             36038..36817
                     /locus_tag="PKI50_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNT02980.1"
                     /translation="MVNSTKEMKVAIHDLTWPEVDKMRDQVKTVIIPVGSTEQHGPNL
                     CISTDATRAYKFSLRVAKALYPHVLVAPCIHVGISGHHMDFPGTITLRPETMISTITD
                     YVSSLMKHGFKKFFIINAHGGNEGTLQVAVENIRTDFGIRIPFVNYKVLCQDVTAEIV
                     GTKRVDHSGEFEVSDAMFLAPEIVREDAICKGDDGNFPLVYTDIYGKYRINYPLNWKQ
                     VAPSGAMGEAHKATPEKGKQLNDAALKRAVEFLEQFMEDED"
     gene            36899..37624
                     /locus_tag="PKI50_04365"
     CDS             36899..37624
                     /locus_tag="PKI50_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT02981.1"
                     /translation="MYINQELCVQCHACIPYCPMKAIEYKEESKRVEINIDECVECEI
                     CGNADVCPTGALTMEEMKWPRILRNQFSNPLITHPDTGVPGRGTEEMKTNDVTGRFKR
                     GMVGIAIEMGRPGTGTRFYDVEKVAMAVAKVGVHFEPHNPVTKLMTDNTTGKMKDDVL
                     NEKALSAIVEFDIPIEKTKTVLEQLKTVTPLINTVFSLDLACRLNEDGSNPAVCLAEE
                     AGYKVSVNGKTNVGLGRPLAKEE"
     gene            37627..38250
                     /locus_tag="PKI50_04370"
     CDS             37627..38250
                     /locus_tag="PKI50_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02982.1"
                     /translation="MTHTLHRKGDKEGLSNDYIVFAISAQTVNAKGSAPKFKEFGDIV
                     LKYNPVNYGDMKTGNAYNVGVDKVHESYKDNSIVHAVFTEEDIVAKVLKELREADLGL
                     SIVLSGIVEHTSECCHKISIEPHTIEYSLGIHGKTDSLPEDNVVEISTMCGHGMVSFS
                     LIEHFCEEIEKGRITADEAARKLAKQCHCGVFNPTRAAEILRLMTDM"
     gene            38492..38884
                     /locus_tag="PKI50_04375"
     CDS             38492..38884
                     /locus_tag="PKI50_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HNT02983.1"
                     /translation="MTIYGAAIIAGCMFVGSFIGDALGGLMGIDANVGGVGFSMLFLI
                     LITGSDLFGKKSSAATSEGLEYWQSMYIPVVAAMTASQNVVQAVSGGVMAVLAGLIAV
                     AVGFALLPVLNNMFKSYEVERETEVKGE"
     gene            38881..39651
                     /gene="madM"
                     /locus_tag="PKI50_04380"
     CDS             38881..39651
                     /gene="madM"
                     /locus_tag="PKI50_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HNT02984.1"
                     /translation="MNAVFEVAQNIMINNGLVMSFLFIGLLMWFSTIISKYLTKGRIH
                     SSAIAIILGLVLAYVGGVLTGGNKGIADIAAFTGVGVLGSSMLRDFTIVSTAYGASIS
                     EIKKCGIVGIISLLLGVVSAFLIGAIFAVCFGYADAVSITTIAAGTVTFVVGPVTGSA
                     LGASSPVITISIAAGVVKSIAVMVVTPFAAKRIGLTTPKAAMIYGGLMGTTSGVAAGL
                     AATDTKLVPYGAMTATFYTGLGCLLCPTVLFWLTRLMF"
     gene            complement(39750..40700)
                     /locus_tag="PKI50_04385"
     CDS             complement(39750..40700)
                     /locus_tag="PKI50_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT02985.1"
                     /translation="MKNYYYDYVLALAEERNFTKAAKKLCISQPSLSQCITKVEKELD
                     VSLFNRSTNPIEITEAGRIFIDTAYKIRTLQYDLENKTSDIADFKNSSLTVGMTPYRC
                     STILPGILKAFTTSMPKVKFHLITGTMQELSNYAQEVSIDIFVSTESYLNKNIFNYKV
                     IAREIILLTVSKSNPINNKLKSYQLSYDDIVNANFDSKKMPIELLSYFKDEPFLLVNT
                     SQHLYKKSVELCRLSKFEPNPFLITANIHTIFNLTLSGLGVSFTPHSFVKYGNLANHP
                     IYYYLDNVNSSRNLIAAYKKNHYLSKPARKFINIMKAVLN"
     gene            41126..42124
                     /locus_tag="PKI50_04390"
     CDS             41126..42124
                     /locus_tag="PKI50_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNT02986.1"
                     /translation="MKKLVSLLMVVILIVALSGCSGTTGGPAEEAKEPAEKSSKYPSK
                     AIEIVVPYAAGGGVDMNTRAIQPFLEKELDTTVVVMNKPGGGGVLGVTYAIMSEPDGN
                     RLLLTNLIAMCSASALNTLQVDLLEGVEYIGGTVVDPGVIAVSKNSKFNSLDEIIEEL
                     KKNPGSLSFGVTGETSLDNLICKNLEVITGAKFNTVSFDGGSEAMAALLGGHIDIMGG
                     TLSEMLTYHKSGEVKSLAVGHGERIDSISDVPTFDELGYKMAVTAAKRAIMMPAGADE
                     EIVKVLQDAVKAAASSEEFAKECESLGLMASYFTPEECVKTVTEVSEFLNGLYGKK"
     gene            42364..43833
                     /locus_tag="PKI50_04395"
     CDS             42364..43833
                     /locus_tag="PKI50_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNT02987.1"
                     /translation="MRIQNMEMLQNHGNKVGRVHVAQMLEAGMNMVDSYIGASRLISR
                     KDNWLIFDGKEFEMDNDPRSGTAKYDLDKFDRVIVIGAAKGVQRCAILLEEVLGEYLT
                     GGHVIGKHGDDIICKKIGVTLAGHPIPDQMCVEGCKKIHEWIKDINEKDLIITIAGSG
                     VSSLMTWPIAGFTIDDLRNLTHMMQIEKGALTKDLNAIRNHLDRFKGGKILRYMKKAT
                     IVNLATNDLGGASWSSPGIRETYEELMKGNRFLPTIADSTTFQQAVEAFHKYDAWNRI
                     PKHIQKFFTTADPNEETVKMDEYEKMNSRLFKLTSRINTIYPAVIKKAEQFGYNAYIL
                     SDTITAEAVEAGKVISSIALNIQNMNQPLKKPCVLISSGELIVTVGKENGVGGRNQEF
                     CTAAALRIAGSSKIVIGSADTDGTDGPGGFIVDGVRTCLAGAIVDGYTCQEAAEKNIN
                     LEASLKRHDTSSDLWDLGCGIKSTQSISALDLRIVAIME"
     gene            43864..45102
                     /locus_tag="PKI50_04400"
     CDS             43864..45102
                     /locus_tag="PKI50_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HNT02988.1"
                     /translation="MSRIEDIRAFEMLNARGKPTISVELTTSNGIAVEATTPTGTSKG
                     KYEAYELYDGEKRYQGRGVKKAVENVNGIIRKTLIGMDVSNQSAIDQCMIELDGTKNK
                     SRLGGNAILPVSVAVCKAGAAEAGLPLYRYIGGLSAKRIPNMISTVITGGEYSLSGLE
                     FEDYILVSDRKEKSVEDGVEMISSLRRRLEEVLRAKYGAFPDTTGALAPPLSSSKEAF
                     EVMLGVIQELGYDNYFTLGLDVAANELFDESNGSYRLMDRIVSPEELLEYYCSLVSEY
                     PITYIEDGFEQNDFKHFAQLKKAVPQIQIVGDDLFATNIERLKEGINNDSANTLLLKM
                     NQIATVSETIKTCNYAHQNGYDVTASLRSGETIDSFVADLAVGLGTHQIKLGSPVRGE
                     RNAKFNRLMRINYELLSVGL"
     gene            45186..45632
                     /locus_tag="PKI50_04405"
     CDS             45186..45632
                     /locus_tag="PKI50_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNT02989.1"
                     /translation="MSLVQIFSIIMVIAGALGIYGALQLDIVYNNIVGPAVMPLIYSI
                     ALLALVAVYFAKGRNAPKIEWNMMLKPPKSDAWIFYILNIVMFILLYLLGTIPSILVF
                     SVGTSLCLKRMNLVKTIVFSALWVAALYFIFVVYLGVQFSTGILIK"
     gene            45652..47181
                     /locus_tag="PKI50_04410"
     CDS             45652..47181
                     /locus_tag="PKI50_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNT02990.1"
                     /translation="MAETLSYLMLGFQEVLVPTTLLMTFIGVVMGTVVGVLPGLGPSA
                     ALSVLLPMVYGKEAIPSLAMLAGIYYGAMFGGMITSISINVPGESASVVTSFDGYPLA
                     KQGKAGMAMGISAISSFCGGTFGIILLTIMGAALAKFALAFGPPEYFAIYLFTFVAIM
                     TIGSGGKIIESAIALIFGLLVSCIGLDVMTGTARLTFGSVNLLAGVDFLPAVVGLFGL
                     SEIVLSLSEGEKIEIDKKKGNYKQYSFKNVFPKLHDFIHSLPAIIRGGVVGFIVGVLP
                     GAGATIATFLTYNIEKRVSKDPDSFGKGNIRGVAVAEAANNGAASGAFVPLLTLGIPG
                     SATTAILIGAFIMVGITPGPALFSEHPDVAWGLISSMYVGNVMLIIITTAFIPFFIWV
                     LGISQKTLPIIVASLCVVGTYSLNNSVFDVLLMLVFTVIGLFMKKLEFPVSPVIIAIV
                     LGGDLEFAFRQSLQLFQGNIFMSFTRPICVVLYLLSLLLIALPVVGGIKKRLAAKSQV
                     S"
     gene            47267..48541
                     /locus_tag="PKI50_04415"
     CDS             47267..48541
                     /locus_tag="PKI50_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT02991.1"
                     /translation="MSVVSNLVSDVRLPRMVKARQYFDNTEIENVPKELERQFLRPEI
                     YGTIHPGMSIAITCGSRGIDNYPLIIKEIVQFCKDKGAKPFIIPAMGSHGGAVSEGQR
                     LICETNGVTEEYCGCPIKATMSVTRIGVTEDGSFDVCIDSYAAEADGIILVNRIKNHT
                     ALRGDYESGLMKMMAIGLGKQYGASLCHRAGYERMAELVPMIGKTVLNNASVLFGVGI
                     VDNAYDKNYKIAVLTKDEIPIVEKELLKEAKRLMPRLLPGSADVLVVDRIGKNISGSG
                     ADPGITGRLTYKYSGVESFQAGKVAVLDITDETHGNIHGVGYVDVINRRVFEKADLDK
                     TYPNSITSTTLMSDKIPVMMKNDRDTIKCAIKTCQPADPENIRLIRITDTLHISEILI
                     SECMVDSVRHIPNLEITDEIIDWEFNEEGNLW"
     gene            48598..49323
                     /locus_tag="PKI50_04420"
     CDS             48598..49323
                     /locus_tag="PKI50_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT02992.1"
                     /translation="MYINKGLCIKCCACIPYCPMQAIEYNSEKKQVEINANECVECEI
                     CKNANVCPKDALIMEELKWPRILRSQFSNPLVTHPDTGVPGRGTEEMKTNDVTGRFKK
                     GMVGMAIEMGRPGTGTRFSDVEKVAMAVAEAGVHFEPYNPVTILMTDKETGKLKDDIL
                     NEKVLSAIIEFDMPINKTKPVLEKLKSVIPLIDTVFSLDLAYRLTEGDSNLAVKIANE
                     AGFTVSINGKTNVGLGRPLAKEE"
     gene            49327..49947
                     /locus_tag="PKI50_04425"
     CDS             49327..49947
                     /locus_tag="PKI50_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT02993.1"
                     /translation="MTHTLHRNGNFESLSNDYIIFAITAQTVNAKGSARKFKEFEDIV
                     LKYNPINYGDMKTGNMFNIDISKIQEGYRDNSIVHAVFCDEDTVAKVLNELKEADLGI
                     SIVVSGILDRVSECCHEKGIKPHTIEHSLGIHGRVDYLPNDNVLEISTMCGHGMVSFS
                     LIEYLSEQILKDRITVEEAAKKLAKQCHCGVFNPARAESILRAMTK"
     gene            50419..51615
                     /locus_tag="PKI50_04430"
     CDS             50419..51615
                     /locus_tag="PKI50_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT02994.1"
                     /translation="MNYDIQLKNNINKNYIFTLLQNIDLTRGIWMIYLAGKGMSLTQL
                     GLLEMIFHITSFLMEVPTGAVADIFGRKISRILGRVLSLISVVILLAADGFLWFAISF
                     VFTALSFNLESGAGEALIYDSLKEIGEEDRYMKISGRKEVFYQVAGVISFLAGGYMAA
                     KSYNIAFAITIIIGAAAVLQSFSFKEPAVDRKKEEQKVKNIFLNQLKESLRVVKSNPR
                     IVSLIVFTEIILTFCTCIFFYLQNFMKGEGYNEAIIGVVYAASFVAAALTASWVHRIE
                     RVIKEQGILLIIPFVTVACLWGIALSPYHFVFFILLTVTEEIVYIAMSDYINKMIPSE
                     NRATILSFSSMVFSFFMITVFPLVGLLGDRYSLIYAIKCLGGLGIVFVIINSVSLLNP
                     GRKIRK"
     gene            51707..52648
                     /locus_tag="PKI50_04435"
     CDS             51707..52648
                     /locus_tag="PKI50_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNT02995.1"
                     /translation="MKLDRLVSIIVLLLRKERVQARELAEIFEVSVRTILRDIEAINL
                     AGIPIVTYQGANGGIGIAEGYRLDKSVLTENDMSTIISTLRGIDGTIPDSRHGILMEK
                     LRNALPSSQLEALDAKVQQLIIDLSPWGPNELLKYSVTCIRKAIENRNEIEFEYIDST
                     GKRTNRRVEPYTLVLKGQKWYLYAWCHTRQDFRLFKLSRMRGLEVTTAVYQPKKVSLE
                     RLDWEDPWSTSESMVFLRLVFEKEMESIVTDWFGDNVEKLEDGRLLANAVLPENNWLY
                     GFILSFGAGAEVIEPAHIRKILAEISKQIYEKYSMET"
     gene            52707..53165
                     /locus_tag="PKI50_04440"
     CDS             52707..53165
                     /locus_tag="PKI50_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNT02996.1"
                     /translation="MDYNIELKEQGLQPVLSVRKTTSVGNLPREIGKAYGSIMQYLQE
                     LGEQPAEAPYTAYYNLDMEHLDVEMGFPVSKQLAGKGEIKAGSIPAGKYAECVYKGPY
                     AEMAPVYEAMNKWIAEKGLEAVGTSYEVYYNSPAEMPESELLTKIMLPVR"
     gene            53188..53811
                     /locus_tag="PKI50_04445"
     CDS             53188..53811
                     /locus_tag="PKI50_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNT02997.1"
                     /translation="MKYDWKKQDKELYLPKNKPEIVMVPPMWFFMLDGRGNPNNEDFA
                     KAVGVLYSLAYAVKMMPKKGVTPEGYYEYSVFPLEGIWDLAPEARELEALDKDSLIYT
                     IMIRQPDFVTEEIAQRALEAAAKKTKHPLLSKVRFGCIEEGLCVQMLHIGHYDSEPAS
                     FAVMEEFCRANNLNRISKVHREIYLSDARKTPAEKLQTVLRFKVEKI"
BASE COUNT        17060 a         8854 c        12696 g        15376 t
ORIGIN      
        1 ctcctaaaaa aatgttttac tgagcaataa gctttgatat cattatatca gcaactatta
       61 ctataacaag tatgcagtat tttctgccca agaaaggaaa aactgagtaa aggagtgata
      121 ctatgaatga ccaatcatta ttctggctga tttgtttatt gcggtaacaa cgctgtttat
      181 ggctctgttg ttcatgctgg gctataaatc catagtactg ctttttgcaa tgtctgcttt
      241 cagggcagta ggcaccggtg tacagaatcc ggctgtggga gcattcattc cccaaatagt
      301 acccgaagac aagctgacaa aggtgaatgg agccaacgga agtattcagg ctgttatatc
      361 ccttgtttct ccgatgatcg gcggggcgct tcttactatg tccactctgg aggcaatttt
      421 ctttatcgat gtctttacag ctgccgcggc tgtatttact ctgctgctgt tcctgaaggt
      481 gccggctcat gccaaggcgc agaagccgca ggaatcaagc tatttcaagg atttgcacga
      541 gggtattgta tacatatgga atcacagtta tgtgaagaag tatttcatat tctgtgccgt
      601 attctttttc atgcttgctc ccatagcatt tttgactccc cttcaggtta ccaagagctt
      661 cggaagcgat gtatggcgat tgactgccgt agaagtcgcc ttttctatcg ggatgatact
      721 gggaggaatt tttatttcgt cctggggagg attcggcaac aggatttata ccatggctct
      781 tgcaagcttt gtaacgggta tctgtactct tgcccttgga gttgtaccgt tattctgggt
      841 atatttatcc ataatgggca taaccggaac taccataccc ctttttaata ccccttcaac
      901 ggtactgctt caggaaaaag tagaagcgga tttcctcgga aggatattcg gagtattgaa
      961 tatgattgca agctcaatga tgcctctggg gatgctggtg ttcgggcccg ttgccgatgt
     1021 tgtaaaaatt gagtggatgc ttattggcac gggttttgtg atgtttatcc aaggcttttt
     1081 cctgattggc agtaaggacc ttgtggaagc gggaagggca aaggatataa ccggaacatg
     1141 attcattatc atttgttgta gggtgactta tttaagtgct ataataattg caatacatat
     1201 atagcggcta agaaagccat aaagctgaca aataactatt tcaaatttta ttgtactctc
     1261 gggaggctgc aggcaataaa aggggacttt gaaaatgcaa agaaaaaata tggatggatt
     1321 atggatgggg ctaccggact cagggaagca aactttactt cggataagac cgacgccagg
     1381 tggtttgtga aggcatggag cgaatatttg aaaaaggcct tggagaattc caatgacagt
     1441 ttaaggtaca ttgttaagaa tggtattggt tcggttaaag agctatacct taatgaagtt
     1501 aatagggaga atatagataa aattgacttg ccctcggcat cagccggaat ttatagaatt
     1561 ggggaaggca tttttgagta tttgctgatt ggcgatatat gcccggtcat cgaagatatt
     1621 gagggcaata ttaaagaaat taaggataca aagctggaag tactggataa tatagctgtt
     1681 aatgaactga gacggctgat ggccagccgg gggcttagct ttaatgaggc cagggagggt
     1741 atccaggaga ttcttctaaa gcacaggcgg cttaaaaaca ctgataaagg atattggacc
     1801 ctggaatttg agccaagcgc cgttgactat tgcatctatg acaagctgcc tttggagtct
     1861 ataagcaaag ctttgtttat gaccgacgga ttttttgcca ttcattcaag ctataattat
     1921 ttcagtgtta aggatttaat atcattcaca gatatgaaag ggctggagaa agtatataaa
     1981 actctgaggg atattgaaga agaagataaa gagtgcaata gatatcccag atttaaaaaa
     2041 agcgatgatg cctcggcaat atacattaaa tatgaggatg tgcaggctgt ttattgattg
     2101 ctgtataatg cctggtatga caatactgcc atattggttt ttgttttttg agtgttgaaa
     2161 ttttgagtag ggggttaaat aaagtgagtt atttcgattc ctatagagga gtgcccagaa
     2221 gtgtttacat aatatttttt gcacagataa tcaaccgttt tggtgatttt gtactgccgt
     2281 ttctgagtct gtttctggtt aaaaagcttg ggctgagcta tcaagacgca ggaattgcgg
     2341 ttatgctgac ttccctgtca acaattccgg gatcttttgc aggggggaag attgcggatc
     2401 atttaggacg caagaagtct tatgcattct tccaattaag tgcggcagta tgtttgttct
     2461 tttgtgcctt ttcagaaaat ccgcaattca ttatagctct tatatgtatg gcttcatttt
     2521 ctaacggagc agtccgcccg ataatatcag caattatcac tgatgttttg cctgctgaaa
     2581 agagaaaaat gggattttca ttatcctata tggggataaa tcttggcgtt tccctggggc
     2641 cccttgtcgc aggctttctg ttcaaccgtt atattcccct tatatttatc ggtgatgcgg
     2701 taacttcatt gatagcagtt attctggttt tgacaaatat caaggagaca ttgcctgatt
     2761 actctgacaa taaatgctta aacgaagaag aaaaaagtga agaaggcaat gttttttccg
     2821 ttttgataag aaggcccaaa atactgatct ttcttaccat caatatagtt tttaatgttg
     2881 tatatgcaca gcactctttt tcgctgccta tgatgctgga taatattttt ggcagcagcg
     2941 gggcgagcaa ctttggagtt attatgagct gcaatgcttt gacggtcatt atgctgacaa
     3001 tgttgatagc ccatgctaca agtaaatgga agcctttaag cgctatcacc atagcagggc
     3061 tgctgtatgc cataggcttc gggatgataa cctttatcag ctcaatgccg ttgtttatta
     3121 tttccaccgt tatctggaca acaggtgaga taatgacggt gacaaacttt ggagtatata
     3181 tagcaaacaa tactccgcaa aatttcaggg caaggttcaa tgcgatatcc tctttaagct
     3241 gggctgtggg ggctgctttg ggcacatcag taatgggcag gtatatggac ttttttggcg
     3301 taaaagccgt atggcctttg gctttctgtc tggggcttgc aggtgcagca ggcatgtatc
     3361 tgcttgaatc tcacagtaat tttagagtaa gcagggcaaa ggaactcaat taatgatata
     3421 ataatttcgt tggatgataa gacgctgggg gatttttatg aacggtgaca tgactcaagg
     3481 aagctcgtta aaagcgctga ttaccttttc cgtacctttg atgctggaga acatattgca
     3541 gcagctttat tatataactg atgctgtgat attgggcaga tttgtcggca aaaatgcttt
     3601 ggcagcggtg ggagtggcca atcctatcat gtccactgca attttcttta tgtttggcgt
     3661 atgtattggg acagctatat ttatgtctca gatttttggg gctgcaaaat atgaaatctt
     3721 aaagagagaa gtatcaacgg ctttaattgc cggtgcggtt tttaccggtt tattatctat
     3781 aatatccgta ctttcagcaa gatatattct aattgctatc ggtactccgg aagagattat
     3841 gaatgatgcg gaaatatacc tgaagatatt atccggaggg ctgatatttt ctttcttgta
     3901 caacttttac tcttttacat tgaaatctat tggggattca aaaaccccat tcattttttt
     3961 aagtatatcc tgtgttctta acggaatctt atgctattgc tttgttgctt tgcttggact
     4021 tggagtggtt ggatcagcag ctgcaacgat aattgcacag gttgtgtctt ctgtaatgtg
     4081 catatcatat gtttacataa aagttccaat gataagcctt aagcctaatg agttggttat
     4141 ggacaagtct ttgttgagaa agacaataaa atacagcagc attacggcat tgcagcaaat
     4201 cagcctgtat atggggaaac tgctggtcca gggagttgtc aatccctttg gaacaaacac
     4261 catagcggca tataatacag taaccagaat cgatgctttt gtgctttccc caggtgacaa
     4321 ctttgtagtg tctgtctcta cttatactgc tcagaacatg ggagcaagga agtggaaaag
     4381 gataatagag ggctataaaa tcggcaatat tattattaca atatataatc ttgctgttgc
     4441 cgcgattgta ttttttggag cggagaaatt aatgcatctg tttatatccg cttctgaaag
     4501 agaggttatt atgataggag tggagtattt aagactaatg ccattatttt atgtgcttac
     4561 aggcttctgc aatatctttc agggtatttt cagaggaatc ggaaagcttc atataacatt
     4621 tttcgcgaca gttacgcagg tatctgcaag agttattctg gctttaatat tcgcccccta
     4681 tttcggcatt tcgggcatat gttatgcagt tggtataggg tgggtaatta tgattatata
     4741 tgagggatta atatatagaa aggaatattt gaggatgaag gcatttgata ctgcgcaaga
     4801 tgttcttgat tcggcttgat aaatatgaag gagggataaa ggtgattgat tatgagggac
     4861 agtatcccgt atatgagatt tcagcctcaa aaataaaaag caatgcccgc aaaatatatg
     4921 agatgtgcag ggccaacggg atcgagccgg caggtgttgt gaagggcttc agcgcaatac
     4981 cggaggtggc ggaattattt atagaggcag gctttaagac gttaggggat tcgaggatga
     5041 aggagattat taagctccgg agggccggta tcaaagggga gcttatgctt atccgaatac
     5101 ctatgccatg tgaaataccg gagcttgtaa gctttgcaga ttcaagcctg aactccgaaa
     5161 tggaaacaat caggcttatc aacaaagaag caggcctgca gggaaaagtt cacggagtca
     5221 tattgatggt tgatttggga gacctgagag aaggcttttt taacagggaa gaggcaattg
     5281 aagccgcgaa ggaagttgaa atgctggaga atgtcaagct gttgggaatc ggtactaatt
     5341 taggctgcta tggttccatc aagccttctg aaaaaaacct gagagagctg tgccggctgg
     5401 cagagttaat tgaaaaagaa atcggaagaa gccttgagat aatatcaggc ggagccagca
     5461 cctcattatc gttattagtt aatggtaaga tgcctgaacg aatcaaccac ttgagaatag
     5521 gtgaggcaat cattactgca aaggattttc cctatgtgtg gggtatagaa atgccgggga
     5581 tttaccggga cccaatggta ctgaaggcac aggtggtgga agtaaaaacg aagccaagct
     5641 atccgatagg ggaaatattt gtggacgctt ttggcaacag gccggtatat accgacaggg
     5701 gaatgcggaa aagagcagta ctggcggtag ggaacaagga tgtcggcgac ttttcaaagc
     5761 ttaagccaag gcatgaagga attgagataa tagggagcag cagtgaccat ctgatactgg
     5821 atattgagga ttatgcaggg gatatcaaag taggggatgt tttggaattt gatacctttt
     5881 atgcggcaat ggtctttttg tgctccagtg attgtgtcag aaaggcagtt gttgagtgaa
     5941 cccctgctga tatgaatggt attcagagca aaataagcag gaatcgggca cttaagtgaa
     6001 ttgaatcctg cttattttta cttcaattaa tgttctacca gactggttac aggcacttca
     6061 agcttatttg aaggttgatc aaggggatga atatcagcaa ttccggtttc ttcgtccaca
     6121 ttttcaatat atatcggtat tccgttatag gttacatgag tcataaccgg tgaagcagta
     6181 atggcttttg cacgtctttg atccatagaa taaacctcct gtatttttta ttaaacatag
     6241 tttttgcatt aaaaattcaa ttattcctat tgaagaaata atttcatcag gaaattacaa
     6301 ctgtaattgc agacagcaaa aagtgccgct aggcattgaa attactagat tcttaattgc
     6361 gcaaaagaat tatttagcgc aatgtgacac aatattacaa aaaaccgcat agtttttatt
     6421 caatcacatt tttcctcaca attaagttta atattgcaat ttagcttgat ttttattatc
     6481 aagtatgaca ttattaataa tatcttaaat aattagtaaa taatgtaaca catatatatt
     6541 tcaataagta ctaaaaatat agtaaacggt agttgaaatt ttgtggtgag ttatgacaaa
     6601 gtatttgaga aataagattt tggaggtaca caagtatggt tggtacagta aaatggttca
     6661 atggtgaaaa aggttatgga tttattacag gagaagatgg cacggatgtc tttgcgcatt
     6721 tttcccagat aaatgcagag ggttacaaat cacttcaaga aggacagaaa gtatcttatg
     6781 acgtaacaaa cggacccaaa gggcctcagg cagaaaatat cactattata taaggttgat
     6841 attttacccc ttgacagcat ggctgtcgag gggtttttag ttaccaaagg acacatgagc
     6901 tatatgcggc tgcagccgca ttttttttgt acaaaattgg gggtattgat tgtacggctt
     6961 gttttgcacc aaacttgtca ggtctgtata tactgtaatg aaaagaaaaa ggaggtatag
     7021 cttgaaagta aaaggtttaa ttatcactgc ttgtatagtc tttgccttag cttctttcct
     7081 accgggatgt tctgtatcat caagcaataa tacaaatatt aatcaggaga aggaagagcc
     7141 taaggtgctt acaatataca gcggcaggca ggaaaagttc ataataccgc tggttgagaa
     7201 atttgaagag gataccggaa taaaagttaa tcttatttcc ggaaaagcaa cagaatacgc
     7261 ccacagaata attgaagaaa aggaaaatac tcaggcagac gtatttttat caaatgacgg
     7321 cgagatattg gaatatctcc gcatagaaaa tatgctgtca ccgattaatt caaagctttt
     7381 agaggaagtt cccgggaatt atgttgggaa ggataatact tggaccgctc ttaccataag
     7441 atatcgtgtg tttatataca atacggattt aataacattg gacgaaatgc cggactccat
     7501 ttttgacctg acggatccaa aatataaagg gcaatttacc atgaacagag caggaaatga
     7561 atcgatgatt acctactttg cttctattca gttaaaatta aaattattat agtataatat
     7621 aattaagata aaaacagtat agaggatata cgatgggagg aaatatgaaa tgataaaagt
     7681 agttgcaaaa agctttgcaa agccggacaa gcttgataag attctgaagc tgtcggcaga
     7741 gatggtggaa aagaccgtta aagaagaggg ctgtataaag tatgagcttt ttcaggatat
     7801 aaaagctcct gaagtacttg ttatcgttga ggaatgggaa agcgaagagg ccttgagcag
     7861 gcatatggct tcggagcact tcagaagaat agtgccccag ctgaatgagc tgagggaaaa
     7921 agcatcggaa gtcaatatat taaaaaaact cttttgaatc aatgtttcag gccccgcggc
     7981 gacatttgcc gcggggcctt ttattttatg aattctgttg tgaattttgc tgctgattct
     8041 gctgctggtt gttttgtcct ttgcccatta tctgaggcag ctgatttatc agttggttca
     8101 cattgcccgg attggcgttg accggcagga tggagacatt ctcaccatct accagtgcca
     8161 aagcatcggt actgattctg gcaccaaggc ccagagcatc cattcctgca ttgccggttc
     8221 cctggtttga gcctgcagct cctccggaca ttttaccgga gctattgccg cctccgtatc
     8281 cgattgttac ggagataatg ggtattaagg tcttatcccc ttgggaaaca ggctttccca
     8341 acagtccttc attttgagta aaggtttcaa tattagtgaa cagtgcatca atattctggg
     8401 ttagagaagt attaacctcc atagttttcc tccttagata taattacttg tcaaataagc
     8461 ttcattaata agcggcccaa gttgagcttt gcagtggcat ccagattgat atagtcgctg
     8521 tttgccagaa agtccggttt ttgatgcagg gattccacat ttaataactg tgaaaccgca
     8581 cttactgcac cgcaggccag gcctgtaaca aaaggatccc ggaacccgta ttgagtattc
     8641 acatggattt cggttggttt aagacttttg agaatattgc cgctcctttt ggtatattgt
     8701 tttggtttca ttaattttgc taaaaccctt tttttcagaa gatatacaag taagaggaat
     8761 ccattgcctt ccttaactat cactgaccta agcagaggat gaagccatct taatgtcagg
     8821 tgcatatctg atctggcaga gtcatagctt agatcgattc ttgatgccac ggtaaacaat
     8881 aaaaaaataa aaagcagaag agacagtaac agtataaaca gtaaagtcat ggaattaacc
     8941 tccatgtacg catttgttca gcctttaata gtttaaccat ttcggactta tttactgtgt
     9001 cagaatatac tgcctcttac atatatgact atacaatatc ctgtcattta tttttttgct
     9061 tatatgaagg ctgccgtcac tttgcaattc agcctggtat cagcaaaatc gcagtaaaaa
     9121 aacaaaaaaa taaaagagct tcaaaagaag ctcaaagaac agcaatgtgc agccgctata
     9181 ttccggacaa tacctccaga acttcattaa tatccggcag ctgactgata ggatatacct
     9241 taatccatct gacaattccg ttttcatcta taataatatt tgctcgctct gatattcctt
     9301 ttttttctat aaaaatgccg tagtcccggg caacctgtcc atggggccag aaatcggaag
     9361 gaagactgac gttatcaatt gcaattactg cactccaggc tttttttgaa ggtaccgggt
     9421 ccacactcag gccaaggggt acggtattga ggctatggaa tttctcccaa ttgacttcca
     9481 aggacctcat ctgatcggtg caaacggaag tccaggcaag agggtgccat gaaagcaaca
     9541 ccttttttcc tctgaaatca gataagcgta ttatttgctc cttgttatcc ttaagggaaa
     9601 aatccggtgc attatctcct attataatac tcactacaaa ctcatctcct ttcttgatta
     9661 tgtattaatc ataccataag tattgtatca gggaaacagg gggtttggca caaagttcct
     9721 atattgtcat attatatcga tttaaggtat aataagagaa aagaagacag aaagaaggtt
     9781 gacagtatgt ttaaaagata ttttaaagta tgtcagctgt tcttagattc atatcataaa
     9841 ggcttgttat gggctagccg ggagactggc ttcgtctgct gcgtatccgt gaactacatg
     9901 gactggaaaa tatactcaat ggaagaatat aaaaacttcg taataacaat tgtgcttata
     9961 ttctcgcttc ttattatatc cgttattatg cttgcagcct atataaacag aatcagcaag
    10021 ataaagagag agctgcagat aaaaaatgaa gagcttacag catccgacga taagcttaaa
    10081 aagcagcttg aagagcatga gagagttcag cacagtttga taaccagtga gaaaagatac
    10141 cttttactgt ttgaaaaaat gatgaacgca ttttttatat ttgaaccggt tattgacgag
    10201 gaaggaatta tttccgatat acgctttctt aaggtaaatc cggggtttga actccataca
    10261 ggtctgaaag gagctgaaat aatcggtaag aaatggatgg aggtatataa ttatcccaat
    10321 aaaaatcttc atatatacca tagaattttg ctgaccggaa agcctgaaca ttttgaaacc
    10381 tactatgaac aggaggataa atattatctg accaatggat ttaagatatc agacaacgaa
    10441 gttggggcaa tatttgaaaa tattacacga tacaaaaagg caatcaaaga aataacaatg
    10501 cttaatgaag aactggagca taaggttgca gagagaaccg acgagctgca aaacgcgtta
    10561 aacgagcttg aagcttttac atatacagta tcccatgatt tgaaatcacc cctcagagcc
    10621 atagaaggct acagcaagtt tgtaattgaa gatctggaat cgggaccggg cgaggaaacg
    10681 gcggagatga tacataatat caggaatatc agcctggata tgatagaaat gataaacaag
    10741 ctgcttgaat attcaactat gactaagact gaaatagcaa aggaaagcgt tgactcaggg
    10801 gagctgttca agtcaataat taatgaatta ttatcagtca atacagacag aaatatagag
    10861 ctaaaggcgg taacggagct gcctgttgtg tttgctgaca aagtgatgtt taagcaggtt
    10921 ctgtacaata tactgtccaa tgcggtaaag ttcacaaaac ttaaagaccg ggctattatc
    10981 gaagttggct gctcaattaa tgagggagaa tacattttcc atgtcaggga caatggggtt
    11041 gggtttgata tgcaatattc cgggaagctg tttggcatat ttcagagact ccatacaaaa
    11101 gatgagtttg acggaagcgg tatagggctt gttacggtaa aaaagattat acagaaacac
    11161 ggaggcaggg tttggataaa aggcagcatg ggacaagggg caacagtata ttttacattg
    11221 cctgttgaaa gaggtggaat atatgctgca ggtaatgatt gtggatgatg tggatattgt
    11281 aagaaaagag ctgaaaaggc tgaagctgtg gggtgacaaa acaggctttg tcatctccgg
    11341 tgaagcatgt aacgggcatg aggcattgga aaagctcgag gaaagcaagt tcgatatggt
    11401 tattacagac ataaggatgc ctaaagtaga cggtattgag ctgcttaaag aaataacagg
    11461 gagaaagctt tgcccctgtg ttgtactgct tagtgattac agtgaatttg tgtatgccag
    11521 acagggattg gtattgggag cttttgacta tataatcaag ccggtggcag aagaagaatt
    11581 ggaaaagctg cttcaaagag caaaggaata tatatccgag aaaaggcttg aggagcaaag
    11641 gattttacag ctggagcaaa agctggtgga aaaggtggat atgtttttcc cgcagtcgga
    11701 ggttagccag cttattgatg ccataatggc aggacataca aagactattg aaaatgcagg
    11761 gagcatattt gaaatagttt gtacaaatat gaattgtgat ttgattaagg tgaaaagcct
    11821 gctaaacagt ataatgcttg aaataaccaa taaaatatca gcgagctgca aatggctgga
    11881 taaatatacg gatattaatg ctataagaac gattgatttt tccggagcaa atgatttgaa
    11941 ttcaataaaa aacagatttg tttcggctgt tgggagaatc tccggcttgt ttgcgagatt
    12001 gaaacacggc acggaggata acgggattac aggtcaggta tgcaggtatg tactggagaa
    12061 tattgacgga aatataacgc tgagtgcagt atccgaatcc cttttcatga gcaaatccta
    12121 tatcagcgag gtttttaagc agaagacagg cctatccttc atagagtatc tgactattgt
    12181 aaaaatggaa agggcaaaaa agcttgtcgg ggacggaagg ttaaagacat atgaggtggc
    12241 agagcttatg gggtttaagg atattgagta tttcagcagg ttgtttaaga aatatacagg
    12301 tataacaccc accgaatatc gtcagggcat ggaaggagaa aaatataaag acccgaaaaa
    12361 attcggggta taaacccata atttttcatt catgaaataa ggataattaa gtattattat
    12421 acagtaatac tgattatcct tattttatag gagacaaata tgaagcagat tgatgtattg
    12481 gtggtggaag actcattcta ttcagcagat ttgagtataa gggtattaaa aaaagcggga
    12541 ttcaatgttc ggtataaaat tgcttcaagc agtataacta tgcgagaagc tcttaaggac
    12601 cggcattggg atcttatact aagtgataat tatatgccgg gttttgatgc acttcaggct
    12661 attgagatca gagaccaatc aggaagcctg gctccctttg tgattgtctc ggagtatatt
    12721 caagagatcg atatcaggaa agcaatggaa aaggggtgtt gtgcttatat agccaaagag
    12781 gatctggata agctggcgca gcatattgaa aaaattttgg aataataaat ttagaaatcc
    12841 attgatgtaa tatttgtgtc acaggaggaa ctaatggtct ttaataaaaa tgaaaaaaaa
    12901 cttgaaagga ttgaaaaagt gacttttggt aatgagtatc gggaattttt aggaaagggt
    12961 gagttggaat ccaatcagat atcattaaga atagcactta cttatatgat tgtagtggca
    13021 ttgtggatac ttttatcgga taagatactg ggattgcttg cggcggataa tgcagtcata
    13081 accaggataa gcatgataaa aggatgggtt tatgtattaa tatcaggcat attgattttt
    13141 gttatagtca gttattcact taagagagtg aagattgcag agggaaagct ttatgacagc
    13201 tgtcagaacc tgagtgaagc aaatatggaa ctggaacacc ttgcatacca tgaccagatt
    13261 acaggtgtcc ggaataaaat ttcactgata aagcgtatta atgagcttgt cagttcagat
    13321 gacagcaaga agattgcaat tcttattgca gatattgata attttaagta cattaatgat
    13381 gcaatggggc atagcatcgg cgatcaagtg ctgattaaat tcagttctcg tcttaaaggc
    13441 ttggctggag tggattctac cgtatacagg cttggcggcg atgggttcgt aatagccttg
    13501 gagggctatg aggaggcatc ttctgttaaa aggctggcag aaagtttgtt gaaggacttt
    13561 aagacccccc ttgatgttaa cggcagtaaa ttattcatta caataagtat aggagtttca
    13621 atgtatccgg aaaatggaga cggtatggat gaacttctta aaaatgcgga tattgcatta
    13681 aataaggcaa aagcaacggg gaagaacaga gtggttttct ataatagaac catgaatgaa
    13741 gcagtatcag agagagtaaa catagaaaag catttgagag cagcgttgat taataacgaa
    13801 tttgaattgt attaccagcc ccagtgggat attgtccaaa ataaggtatc aggcctggaa
    13861 gcgctgataa gatggaaaaa ccctgaactt gggtatgtaa atccccttaa atttatcaac
    13921 attgctgagg atacacatat gattattcct ataggtgaat gggtattgaa aagtgcttgc
    13981 acgaccctga aagaattgca caggcaaggc catgaagggc tttccgtatc cgtcaatatt
    14041 tccatggtcc agttgctcca ggatgatttt gtatgcaaag taaccggtat tcttgattat
    14101 gccggcttga gtccggagta tcttgagctt gaaataacgg aatcagtatt gatggaatcc
    14161 tgtgatgcat tggaagggaa gctcaagatt ctgaaggaca tgggaataaa aattgcattg
    14221 gatgactttg gaaagggata ttcctcgctt aactatttaa agcagctgcc gatatccact
    14281 ctgaagatag ataaatcatt cattgacacg atagagtccg atagtaagca taaatccttg
    14341 acaaatgtta ttgtaaagct gggaagaatc atgggactga gtgtagtggc ggaaggtgtt
    14401 gaaactaagg agcagttgga ttatcttacc aggcacaggt gtgataaggt ccaggggtat
    14461 ctgttcagca aaccggtgcc gggggaaaag gtcatcgaag ctataagagg gctgaacgtc
    14521 agttttcgct gatcagcaaa aatatatgaa atatttatta aaaagcggtt cgtgaagtgt
    14581 accgcttttt tttcggcaaa atgcatatga aacttatcag ccttataatc actctattat
    14641 tgattttcac tgtaatggtg agatgtacgc tgcagaaagc cggaaaaact tttattaaaa
    14701 cagtaagttt ggaaaaagaa aacattcagc aagaaaattt gatgaattat tattaaggaa
    14761 cctcttatct ttgttgacag tgttggggaa aattaatata attaccgtaa gtatgggaca
    14821 gttcctgact gtcccaattt actgaaaagg acggagtgga tcggatgagt aagagacctg
    14881 ttgtattggt tgttatggat ggcataggaa taaatgaaga ggaaaagggc aatgcagtga
    14941 aggctgcaaa tacaccgact cttgatatgc ttatgaatca cagtccgtat acattgataa
    15001 aagcccacgg cacggccgtt ggcctccctt ctgatgagga catggggaat tcagaggtag
    15061 gtcataacgc tttaggcagc ggacagattt tcagccaggg ggccaagctg gtaaatgagt
    15121 caatagagaa tggaaaaatg tacagttccg atacatggcg aaagatagcc ggaaattgtg
    15181 ttgctaacgg ctccgccttg cattttatcg gactgctgtc agatggtaat gtacattcca
    15241 atattgccca tttgaaatca atgattatca gggccaagga ggacaaaatc aaaaaagtga
    15301 gggttcatgc cctgcttgac ggcagggatg tactggcaac atcggctttg gattatgttg
    15361 aacagatgga aaagctgttc aaggaactga acgacggcag ctttgacgga agaatagcca
    15421 gcggcggcgg aagaatgaag attaccatgg acagatatga ggcaaactgg gatatggtaa
    15481 ggcttggctg ggatacccat gtatcgggca agggcaggca gtttggttct gccagtgaag
    15541 caatatgcat atatagggag gaactccccg gggtaattga ccaggatctg cctccttttg
    15601 taattgcaga aaacggcatt ccggcaggta ctatcaatga cggagacagt gttgtgctgt
    15661 ttaatttcag aggtgacagg gcgttggaat tgagcatggc ttttgactat gaggattttg
    15721 acaagttcga aagaggcagg taccccaagg ttatattctg cggtatgctt cagtatgacg
    15781 gtgatttgaa gctgcctaag aatttcctgg tctctcctcc gaatattaag aatacattat
    15841 ccgagcatct ggttaagcac ggtataaagc agtatgcagt atctgagaca cagaagtacg
    15901 ggcatgtgac atacttctgg aatggcaaca gaatcgagaa gtttgacgat aaacttgagg
    15961 tttttgagga gataccttca gacagggtat cctttgatga acgtccctgg atgaaggctg
    16021 cggaagttac ggataagctt atagatgcct tgaaatccgg caaatacgac ttcttgagag
    16081 ccaattaccc caatggagat atggttggac ataccggcag ctttaatgcc accagaatag
    16141 cagtggaagc cgttgacctt tgccttgcaa gagtcataaa agcagcggat gaggcccggg
    16201 ccatactcat cattacggct gatcatggaa acgcagatga gatgcttgaa aaaacaaaag
    16261 gaggcactgt aaaggcaaaa accagccata cactcaatcc ggttcctttt ataatacacg
    16321 acaaagtgca aaggcacgaa ataaaggaag ggagcttcgg gcttgcaaat attaccgcaa
    16381 ctataacaac cctgctggga atcgaccacc cggattgctg ggaagaaagt atagtataaa
    16441 aaatctattg acaaataata aaatacgtaa tatattactt aagtcggtaa tatattacgt
    16501 attttttaat tcctcatctc caacatattg gaagagacgc tgaaagatgc cggacaaggc
    16561 ataataagct ttaagcttaa gaatatgaat ataatgccct gcaggtcatg aggagcctgc
    16621 ggattcccgg atataaccag gatagagcct ctgctgaact gctgcaggct atttgccgaa
    16681 gaaactgaaa tgaggtggct tgggggcctt gccttcggag gggggccgtt gataaacgga
    16741 gcttttatac cggcccttgg cggcttacat gaacaataac gccggaaagc aggcacgtct
    16801 taacggaaat atcgattatg caagaaaggt ttatcttgaa taggccaaaa acagtaatat
    16861 atagtcacgt ataagcatac tttgactata cattactgtt ttttcttttt attatgtcag
    16921 tgcactccgt cggctatttt acataattat caaagtagcc ctgaatatac acaatagggg
    16981 tacctttatc cccgctgcct gaggtcaggt cgcagaggga gcctatcagg tctgtaagct
    17041 ttctgggggt ggtgccttgg gcctccatgc agcctaccag gtttgagcct ttgcacctaa
    17101 tgtattcaga tacggctttt ttcaattctt ccccctttaa atgagcaaag ttattatcgg
    17161 caatatactt cagttttatt tcgttaggcg tcccttccag tccggaggta tatgcagggg
    17221 ataccacagg gtcggcaagc tcccaaatct tgcacaccgg atccttaaaa gccccatcgc
    17281 cataaatcat aacctccact gtcctgcctg tcttttcttt cagcatagct tgaatttttt
    17341 caactatggg ctggcagttg cgggggaaga gctttacgct gctttccgtt gatttgtttg
    17401 aacccagcag tccgtatacc tcattgaagc cgctgccgcc aacgggtgaa gacataatgt
    17461 catccaagcc gtataccttt tctccgccgt tttccttcag tattcttttt gtcctggctc
    17521 ttgtgtgaat gtcgcagcag agtacatttt ttgtatgctt gagaatggtt aaggggttgt
    17581 tggagaatat tatttcacac tcaatgcctt gttcgttgat tatggattta taatattcaa
    17641 tataatcaac accggtaaag gtatgcctgc tgtatccgaa gtatcggcga aaatcacctt
    17701 ctgtcaatac atccgaccag ggattcaggt tcttttcgct gaaggtgtct atatctatca
    17761 attgatttcc cacttcatcg gtgggataac tgaacatcag taccgctttc cctgctcctt
    17821 ttgcaatacc cttaaggcat atggcaaacc gattacggct catgataggg aatattactc
    17881 cgattgtact gcttccgaac cttgatttaa tatcttctgc gatatcatct attgatgcaa
    17941 agttaccctg cgcccgggca accactgatt cagtaacagc aacaatatca cggtcattaa
    18001 tgctgaaacc ttctgattct gaagctttta atacagtatt cacgacaata tctgatatat
    18061 tgtccccctg cctgataata ggggcacgga gccctcttga aatagtacat gttattcttt
    18121 ccataaaaat ctctcctcaa ccgtatcaga ttatcggata tatagtgttt ccatactata
    18181 ctatacaata gcatttgaca taagtaaaat taaaactatt tgtttcaaca attatattta
    18241 caaaaactgc aggaaactgt tatgtttttc atgtttatta tgttataata aatattgctt
    18301 aagatctgtt atattccttc cggataaagt gatatcaggt acggagcatc agcgtaagaa
    18361 gggtgtgcag gtattgaggg gtattatccg gtaaaaaggg gaggaataaa ggaactatga
    18421 ttgagaaaag aagacacaaa aggcttcctt taaaactgga actgaagatt tcttcattgt
    18481 tcaggcagga ttatgaaata attgatgata ttaatgaggg tattgagatt aggaatatat
    18541 ctaagacagg tataggattt ttctgtactc atgagctgcc gctgaactat tattttgacg
    18601 cgaagatcca gcttacaccg gatagccatt tctatgcagt agtgaagata atacggatgg
    18661 aaaagcttga ggaaggatac tttgcaggct gtgagtttat aggattggca gatatattga
    18721 gtatgagggt tgatatgtac gaggatgaac tgaataatat ttagattcgg ggcggtattt
    18781 attccgcccc gtagttatta aattattcaa gggggttctc gattagtgtg gcaaattgaa
    18841 aatcatgatc gtggcaggcg tcaaaggcag tcgtatcact ggcgaagtga acatgcctgc
    18901 cgccgtcgac cggaacctga agacctgttg ttcctcctac cccgtgtata tgaccgaaga
    18961 agaagtctgt atcggtcatg aaggtgtgca catgtcctcc gggaacaaat atcacttcac
    19021 tggtgactcc tgcaaatctg tggttatgtg cttctacgtc ttgtgaagga agcattgtac
    19081 ttcccaaaaa ttcatgatta tgggtctgag tctccttgat gaatttgtat tcatatgagg
    19141 attctgtctt ttgtacataa ttcttttctt tttttgaacc gaaagttacc attggcatca
    19201 ttatttccct cctttcgctg caatattttg aatacaacgg attgtcccaa tacaaaatat
    19261 gcagcagcct gagggatgtt actgttaatt aagaggacac gggggacacg gggacgtttc
    19321 tcctgtcccg ctttcagcgg gacaggagaa acgtccccgt gtcccccccg tgtcctctta
    19381 aatcctgtta gtctttgtaa tttgaaagtg cagtacttgt ggtgttaatt gcattttcta
    19441 tggcattcag ggtattttga atctgctgct tgttttcagg tttttcggct gaattaagag
    19501 cctgattcaa tgaggtttta gcattgttaa gctggctgaa aacctcctgt acttgctgtt
    19561 ttgcattctt tcccgacatt tattcgtacc tccgtatgtt tataatttga tttacaatat
    19621 tattatctta attttgcagg aaataatgct tgaactaaat ggatgttaag acaattccta
    19681 tcaagttgcc acattgtaca acaaatgtta aaataagcta tgatgtatat aaaattgccc
    19741 ggagggatat aatatgaagc tgatttcatg gaatgtaaat ggtctcagag cctgtataca
    19801 aaagggcttt ttggaatatt tcagagaagc ggatgctgat gttttctgca ttcaggagag
    19861 caagctgcag gaggggcagg tggatttgaa aattccaggc tatcatcagt attggaatta
    19921 tgctgagaag aagggctatt caggggttgc cgttttcagc aaagctgagc cgttgaactt
    19981 cacttgcgga ataggcaggg aagagcatga caaggaagga agagtaatta ctcttgaata
    20041 taatgatatg tatattgtta cggtatatac ccccaattcc caggaggaac tggcaaggct
    20101 gggctacaga atgcaatggg aggatgagtt caggagttac ataaagggat tggacggcaa
    20161 gaagcctgtt gtggtctgcg gggatatgaa tgtggcgcat aaggaaatag atttgaagaa
    20221 tccgggcagc aacaggagaa atgcaggatt caccgatgag gaaagaaaca agttcagcga
    20281 atttttggaa gcgggtttta tagataccta tcggtatttc taccctgaca gggcaggtgt
    20341 ttactcctgg tggtcatacc gcttcaatgc aagagctaat aatgccggat ggcgcataga
    20401 ctatttttgt gtttctgaaa ggctggccga gaggctgatc agcgcggata ttcattcgca
    20461 ggtactggga tcggaccact gtccggtaga attgattatg aaataacgag gcgaaaagat
    20521 atcctccatt tctataagtt gtgggtaccg ttttcgttgt ccattaggcg gttaacattt
    20581 ttcccgcctc gttgaaacgc ccgggtaggt ttttgcaagt gtgcaaaaac ccttattgaa
    20641 atcttgttat aacagaacag gggatgcata tgattaaaac ttttgatgag ttgggaatgg
    20701 atgagcgtct gattgaaggc ttgaaaaagc aggggatcac ggaaccgaca ccaattcagg
    20761 ccgataccgt taaactagct ttggaaaaca aggatatagt cggacaatca cctacgggaa
    20821 gcggaaagac tctggcattt ctgcttccta tttttcaaag ggtggatgcc ggcaaaagag
    20881 agaaccaggc aatagtactg gaaccgaccc atgagttggt gatgcagata gaaaagcagg
    20941 caaggctgct ggctgcaaac tcaggcattg ccgttacttc cgcagcaatt ataggagaag
    21001 tgaacatcac ccggcagata gaaaagctga gagagaagcc caatataatc ataggttccg
    21061 caggaagggt gctggagctt attaaaagaa gaaaaatcaa tgcccagacg gtaagaacca
    21121 tagtcattga tgagggagac agaatgcttg acgagcacaa tttgggcact gtcagggagg
    21181 ttataaaaac caccttacgg gacaggcagc ttatggtctt ttccgccacc ataggtgaaa
    21241 aagccttgag taccgcaagg gaattcatga aggatcccca ggtagtccga attgaagaca
    21301 gaggaaaaat aagtccggat atccggcata tatacctggc agctgagaaa agggataaga
    21361 ttgaagtact tcgtaaactg gcggcatcaa tgaagcctga aagagccata gtatttatta
    21421 ataaaagcga ggagctgcag ctgacggctg caaagcttaa atatcatcat ctcagcgctt
    21481 gtgatatatc cggcagctct tcaaaggaag agcggaaaaa agcattggag ggcttcagga
    21541 agggtgatat aaagctattg gttgcctccg atatagcagc cagagggctg gatataaaag
    21601 gggtaacaca tatattcaat ctggacctgc cggaagaccc caaaggatat ttgcacaggg
    21661 caggaaggac aggaaggatg ggggaacccg gcacggtagt ctctattgtt acagaaaggg
    21721 aggttccttt cttaagaaaa tgtgaaaggg atctcaaaat aaaaatatat gaaaagataa
    21781 tcgccagggg aaaagtgctg gatcctcccc aaagtaaaac gtcagcagga aaaggggccg
    21841 ggagataagc tgatataccg catatagctt cccggagagg cgttatatat ttggaataaa
    21901 taaaccaaat atgaatattc atgatataat tactttttaa tcattcattg tattataata
    21961 ataaatgtta ttaatgagtg attaatgaaa tgcacatatg gaggaggcag cagtattaaa
    22021 aatcttacga gtagaagtgc atagggatgg gtactttata gatcgaaaat ttgtggggtt
    22081 ggctgacata ccaggcatta aatctattta tatggtgatg aatagaaatc aaaaatgagc
    22141 ggtcaatgtt tgtactctgc tagtattatt gagataggag ggattacgtt gataaaaatt
    22201 ggcataatag ttcctaacga tttagtaaat attgcaaaat cagctgcaga gggaattgaa
    22261 gatgaaataa cgattttata tggttcaatg actgaaggaa ttagtttagc taaacaactg
    22321 gaagagtgca actatgatat tattattacg cgtggcggga cacaagtttt attaacccag
    22381 tctaatatta atataccagt tatatcaatt ccaataactc cgatagatgt ttatgaagct
    22441 gttaatgagg ctgaaaagat tagtaaagat atatcgttta ttgtatctca aaatatgatt
    22501 tcgataatcg aaagttacat aaaaataagt ggtagaaatt ttaaggtatt tcaaattaaa
    22561 gaagaatcag aaattgaaaa aaaaataagg gaacttgcca cagaagggaa aaaggtagta
    22621 gttggcttag gtacgatagc taaatatgct agttcatatg gtttggtacc tatagtgatt
    22681 aaatcgggca aggaatcttt tttatctact attatggaag caaagagaat tgttaatgct
    22741 acaaaaaaag aaaagaaaga taaagaaaga ataaaagcca ttattgagca tactattgaa
    22801 ggtataatat gcgtagacaa gaaaggtcat ataattatta tcaatgaagt tgcacggaaa
    22861 ttactcaaat gtgataataa tgggttagta ggtaagatga taagttcggt tttaccggat
    22921 ctccaacttg aagatacttt atataatggt ataatggaga cggaagtcat taaaaattta
    22981 aaagacacta aagttatggt ttataaaata cctataattg tcgatgacga gatagaaaat
    23041 gcggtagctg ttttgagaga tatagatgaa atacaaaagg cagatgaaaa aatacggcag
    23101 gacatagctg taagcggtca ttttgccaaa tatactttta acgatgtaat agggtgcagc
    23161 gaatctataa gagaaatagt cagaatagga aaagagtatg caaaggttaa ctctactgtt
    23221 ttaatagaag gggaaaccgg aacgggtaaa gaaattattg cacagagcat acataattac
    23281 agcaatagat ctaataagcc gtttgttgct gttaactgtg cagcactatc ggaaagcttg
    23341 ttggaatctg aattatttgg atattcctcc ggtgctttta caggagctga tcgtaaaggt
    23401 aaaagaggct tgtttgaaca ggctcatacg ggtactatat ttctggatga aatatctgag
    23461 ataaacccat tattacaagg aagattgctt agggttcttc aagagaagca agtaatgagg
    23521 ataggagata ataagcttat tcctgttgat gtgagagtta tttcagccac gaataaagat
    23581 ttgcataacc ttgttgttgc caaaaaattt agagatgacc tatactacag gctcaatgtc
    23641 ctaagaatag aattaatacc tttaaggtac agaagggaag acataccttg tctgatagat
    23701 tattttctaa gagcttattg tgcaaaatta ggcaaagaga atattgtttt tacttccgaa
    23761 gcagcagaac atttatcaaa gtatgactgg ccaggtaata ctcgtgaagt cagaaatttt
    23821 tgtgaacgat tggctgtaat ggcaaaaaaa acaataatta cattagagga tgtaaccaat
    23881 catataataa atattggggt taattacaat aatgcagatg aacttgttga tattgtcgga
    23941 aaaaataaaa acctatcaaa agacaactta aatcttaata acatgacaaa gcaaagtatt
    24001 gaacttgctt taatacattc taatggaagt ataactaatg ctgcaaaaga attaggagtt
    24061 tcaagaacta ccatatggag aaagatgaaa aagtataact tattggttaa aaaatgaaac
    24121 acggctactt aaagcgttgc ataatgaaac acatttgaaa cataaagaat ttaacatgca
    24181 gtagcaatgc tgatattcta ataatggaag ctatgcctta ctagttggca tagcttttgc
    24241 tattaattag tgtaaaagaa attagtgcga aaatcaatat agaaagaatt acattattaa
    24301 gggaggaaat tttatggaaa gaagaacata cttacagatt cctgggccaa caaatattcc
    24361 cgacaggata ttgaggagct tatctcagcc attgataaat catagaggtc cggagtttgg
    24421 agaattactg acaaaatgcg ttgaaggtct taagaaggtt tataaaacaa aaaatgatat
    24481 actaatattt ccgagttcag gtagtggcgt aatagaatcc gcaatagtaa atttgttttc
    24541 tcccggggat acaatattaa cagcttccat aggggtattt agtgaaagag cagctacgat
    24601 agctgaaaaa ttcgggttga atgttattag gataacaaag gaatggggag agtcagtcaa
    24661 acctgaagat gttaagaaag tattagaaga ggatacagac aaaaagatca aaggagtttt
    24721 tgtaccacac aatgaaacta ctgctgctgt tgcaagtgat ataaaaggaa tttcagaagt
    24781 tataaaagaa atcaaccatc cagctgttct aattattgat gcagtaagct ctttagcatg
    24841 tttgcctatt gaaacagatg attgggcaat agatattgta ctgactggct ctcaaaaagg
    24901 tttaatgctg cccccgggaa tgggaattgt atcaattagt cctaaagctt gggaattggt
    24961 agaaaagtca acattaccaa aatggtactg gagttataag gctgttaaag aaagaatgga
    25021 gattaaagaa tttccttata caccggcgac tacattatta ttcggcttaa gtgaagccct
    25081 cgatattatc gaagaagaag gattagaaaa cgtaattgca agacatgaac tattggccac
    25141 agcagtaaga gaagcagcta aagctatggg gttaagtata tttactgagg aaggatatga
    25201 gtctaattcc gttaccgcaa ttattatgcc agaaggtatt agttacaaag atttagctgg
    25261 tatattaaaa actaaattcg gcgttgttgt tggaggaggt ttacagaagc ttaaaggaaa
    25321 aatattaaga atcggacatt taggttctat ccataaggca gatatttatg caataatgtg
    25381 tgctgtagaa gcggcattgt ttgaattggg atataaggta gaacttggat cggcagctag
    25441 agcagtttca aaagtttttt taaatgataa gtaagataaa tctttagctt aaagcagaaa
    25501 tgcaataatt gcttgaatat tacaaaatgg aggtatttaa atgtattata ctactaagga
    25561 aattgaacac aaatttgtta cagatgtttt tatgcatctt ggagttaagg aagaacatgc
    25621 taagatggcc gcagatgtag tagtacagtc agatattact ggtgtaacta ctcacggatt
    25681 ggctcggatg ccgatgtacg taagaagatt attacaaaaa acagttaacc caactccaaa
    25741 tattagacaa acaaatgatt ctgagaatct aatagcttta gatggagata atggtccggg
    25801 aaatgtagta ggaccaacag ctataaatat gtgtatagag gcagcaaaaa aacacgggat
    25861 tgcagctgta tcaatcaaat atagtaacca ctacggtgtt gggaattatt atgcatggag
    25921 gtttgctgaa gcaaatctta taggcattac tatgacaaat agtactccca gtatggctcc
    25981 aaccggaggt aagacaccta tgctgggaac aaatccaata actatagcag ttccggcagg
    26041 aaaacgttat ccaatatgtt tagatatggc taccagtgtt gtagcaaaag ggaaattatt
    26101 aaaagcagtt gaggcaaata gtaaaattcc atttgaatgg gcagttgata aagagggaag
    26161 acctacagat gatccgaatg aagcattgga gggctcatta ttagcaattg gtggatataa
    26221 aggatacggt ttagctatga ttatagaaat tctttcagca gttatatccc aatcagctta
    26281 tggattagat attggagata aatttgatgc tgattgtaca gaccaagaaa aaataggtca
    26341 ctttatgctt gctatagatg tttccaaatt tttggattta gatacattta agaaatctat
    26401 ggatcaatat atagaaacaa tgaagaatac tgaaaaggct gccggagtag aagaaatata
    26461 tgtgccgggt gaattggaat tcttaagagc tgaaaaaata aaagagacgg gaattcctgt
    26521 tccggaagga atacaagcaa agatgctaaa aattgcaaaa gatgtaggaa taggatctga
    26581 tattaagact atagatgatt tgtttgacag atataaataa taaaattgaa gagctgtagg
    26641 tgaaactcta cagctctcat tccatttttt acaaaaagtg cgaggtgcct taatgaaaca
    26701 gtatattgat ttacggtcag atacagttac tcaaccaacc caagagatga gagatgccat
    26761 atataatgct atagtagggg atgacatact gggtgaagat cctacagtaa aggaattaga
    26821 agagctggca gcaaaaatgt tcaataaaga ggaaggactt tttgttagct cgggaactat
    26881 ggctaaccaa ctagcagtta tgacttttac taatagagga gaagaagtca ttgttggaga
    26941 cacatcccat atatacaatt tggaagtggg tggcctatca gccttgtcac aagttcaaac
    27001 cagaccaata catgttccgg atggcattta tgatatagat attctggaag aattaataca
    27061 gccaaagggg atacaatcac cgaaaactgg attaatatgc atagaaaata ctaataattt
    27121 gaatgctggt ttagtagtac cggtagataa tattaatgaa gtatgcaatt tagctaaaaa
    27181 aaaggatatc cctgtttata tggatggtgc aaggatattt aatgctgcaa tagcgttaga
    27241 tatgaatgta aaagaaattg ttaggaatac ggatgcggtt atgtttgcat tgtcaaaggg
    27301 tctggctgct ccttttggag ctgtgctggt aggaagtaag gagtttatta ataaagcaag
    27361 gtggttcaag cagcgtattg gcgggggtta tagacaggca ggatttatgg ccgcaccggg
    27421 agtattggca ttaagaacta tgatatcgcg aatttccata gatcacgata atgctaaatt
    27481 actaggtaat ggcttatcaa atatagttgg tctaagaata gatacaacac gaatacatac
    27541 taatatcgtt tccgggtcca ttgaattgtt gaatattaca attgaggact tcttaaatag
    27601 gttattgagc aaaggaataa aagctaaaag gatttcagaa acatccttta ggatggtaac
    27661 acatttgggt attgatgaat ctcatattaa ttatgttata gaatctgtaa aagatataat
    27721 tactaatttg tgattattca ttgtagccgt ttacataata atcatgatat tgtctaaaaa
    27781 ataatgagga ggatgctacg ggcaatgtag aataacatta taatgaacat gtcattttat
    27841 cttattgaca taataattta tatagaataa aatacttata atactttttg tccatgaacg
    27901 atacaaatta gcggattaat actaatgttt gatgacagtg ttagttagaa ttacaaatag
    27961 aattattacc tatcatgcag ccctgcctag tacattagga aaatcggggg gaggggtaaa
    28021 atgagtgagg atactatact aataggaggg tattcgaact ttcccagcgg agcagctgca
    28081 aaggaagtct atgggatgtt aggtgtagcg cttgagattg acccaaagac gggaaaaata
    28141 gtaaacgctg ctactactct tctgacagat gtgacaaaga attttgtgag ccgaatatta
    28201 gtaggacaaa acatgaacga aggtttaaaa gagccaatta aatcaatcga aagaagatac
    28261 ttcgggagaa ataagaaagc attaatagct gcattaatgg atgcacacag gatctacgaa
    28321 aaatacctgg aggaaaagta taatctttaa aaaaggaata gtgaatgacc gcagggctgc
    28381 tgtgttattt attgtagagt taacaactct atcaaacata aataagtatt caggaggtta
    28441 tgaatactta ttttttattt ttaatagtct gaatattcat cctattaaaa aatcagaacg
    28501 cagtgctgcc cgaatactga aaatcgaagc aatgatttta agcctaaagg gggtgagcct
    28561 atattacaat gggtcattcg ctaaagtatg attcattttt acaatacaag gagggtgaga
    28621 aagtggaaaa gatgtatgct gaaaggcaca acaagctgct ggaggtaatg agagacaaag
    28681 gatgggactg tgtgatttta tggccgtcgc caaacatgta ttatatgacg ggttattatc
    28741 caaaaccaga tgagcgattg cagatagctt ttatccctgt gcagggagag ccggttctta
    28801 tagttccgag attatactct ggcgaatcca agcataaatg ctggataaag gatcaaagaa
    28861 gctggataga tggcgacgat atacttgctt taaccaagga tgtaataaaa gaccttggtt
    28921 taaaagacgc acgcatagcc ctggatgata ctatgggttt tttgcaggta tcaaccattc
    28981 aagcagcgtg tccaaaagct gaattcggtt tagccggtac agtgatgtct ccgctcaggc
    29041 ttgaaaaggg tccggaggaa atcgcgttaa tgcaaaaatc cagtcaaata agcgatgagg
    29101 taatgcaaat aggtatcgat gcatgtttga gcggacaaaa cgaacagcag ctagttgact
    29161 tagtggagta tgaattaagg aaaaggggta tggccaacgg tttcagcaac ctgattgcct
    29221 caggtactca cagtgctctt ccgcaccatg tttcgagcaa aagagttcct caaaagggcg
    29281 atgcggtatt tcttgacata ggcggtgcct atgaaagata ttggtcggat ataacaagga
    29341 ctattcatat aggaccacca tccgctaaat ttattaaagc gtacgaagcg gttaaagaag
    29401 cccagcagag ggcaagtgag gcagtaaagc ccggagttac tgcggaagaa gtgcatgaaa
    29461 cagcctttgg atacctggaa aagaagggtc ttagcaagta tttcttccac agggtcggtc
    29521 atggaatggg tctggaaggt catgagctca tttcgattaa taggggaaac caccttgtat
    29581 tgaaaccagg aatgactttt tctgttgagc cgggagtata cttcgagggt gaattcggta
    29641 tcagaattga agatactgta gtagtgaccg aaaccggtca taaatcgtta actgagttta
    29701 cgcgtgacct gattgttctg taagcattgt ttgtttattg acattagata tatatctgat
    29761 agataaataa aaggaggaga aagtaaatga agagaatgcg ttatcttgca ctggttatgg
    29821 ttcttgttat gctgttgagc gtaggttgta gctcaagcgg cggagaggcc ccgggggcag
    29881 aggcaccaag tgaagcagga agcgaggcta gtactccagc gaaaaccaca ttggtatatg
    29941 gagttggtgt ggatgcaaca accatggacc cgcattttgc gaacaacact ccaaccggaa
    30001 atgtatcatt ccaggtacac gagaatttga cagcttttga tgatccaaaa acaatgaaac
    30061 cggtgggtaa actggcaaag gattggaaaa tttctgagga tgggcttaca tggacattca
    30121 atttaaggga agggataacc ttccatgatg gagcgccatt taatgcggaa gcggtaaaga
    30181 aaaattttga ccggatactg aatccggatt taggtgctcc gcgccgatca aacttcaaaa
    30241 tgatagacag actggaagtt gtagatgagt atactgtaaa cataataacc aatgtcccct
    30301 atgctgcgct tcctcaactt cttacctgct ataacgcaag catattgagc cctaaagcaa
    30361 ctgaagagta tggagatcaa tactctgctc atgcttcagg aacaggtccc tttaagttgg
    30421 gtgaatggat acccggagaa tcaatgacac tggataggaa tgacaattat tggggtgaaa
    30481 agcccagttt agaaaaatta gtgtttaaaa taataccgga ggatgccagc cgtgtaatgg
    30541 cggtacagac cggagaagta gattatattt ccaatgtacc tccctttgaa attgaaaact
    30601 tgaaatctag tggggatgtt acagttgaga tgcacgaagg tttccgcaca atatatcttg
    30661 gaatggtcat taacaggcct ccctttgata acatcaaggt tagacaagca ttgaactatg
    30721 caatcgaccg cgatgcgatt ataaacgatt tcttggaggg ctctgctact tatgcaatag
    30781 gtcccgaagc aacctcaata ccgggggcat caagtgatct ctttaagtat acctatgacc
    30841 cggaaaaggc aaaggctctt ctggctgagg cagggtatcc cgatggattt gaaataagat
    30901 ttgtaagtcc ccatggtcgt tatataatgg acagacagat agcagaagcg attcaggcac
    30961 agcttagcaa ggtaggaata aaagcgaatc tgcagatact cgacttttcg ctgctgctta
    31021 acatgctgcc tacaggaaag gacgcggaat taatgttgat gggtaaaggc tcacctgcct
    31081 ccgatccgga ttttgactta acaaattatt ttactaccga tgggacaaac aactggtatt
    31141 ttatatcgga cccgaaaata gatgagctca ctgataaaca gcgggcaacg atggacttaa
    31201 acgagcgcta tgaggtttta caagaactgc aggaaaaggt acaggaagaa ctgccttggg
    31261 catcactcca ctatgagaga cagatagtag cgttgggtaa gggcgtagcc ggagtgacag
    31321 tatggccata tgagtatatt gactttagta aggcgaaatt cgaataaatc acataaacaa
    31381 gaatagttgt ttgacattgg gaggctactg cctcccaatg acaaactcca ttttataggt
    31441 ggtggaaaat ttgtttaaat acttaaaacg gcgcttatta atgttaattc cattgcttct
    31501 ggtggtatct atcatagttt tttcaatgat ccacctgatg cccggtgacc ctgctatgat
    31561 aattgcaggc gagggtgcaa gccaggcaga tatagaagcg gtaaaacaca agcacggcct
    31621 agacaagccg ttatttgtac agtactttac atggttggga aatattatac gcggtgattt
    31681 cggtaaatca ttacgtaccg ggcgtcctat atctcaggag attggctttc gttttgtaaa
    31741 cacaataaaa cttgctactt tagcaatagt tataggaagc atactcggtc tagtgatggg
    31801 gattgtatct gctaccaaga gatattcatt ttttgataat gttacaatgg taattgcaat
    31861 ggttggtgta tctattactc cattctattt aggactgatg tcaatattat tgttttcagt
    31921 aaaattgggc tggttaccaa tgatgggagt tgaatcctgg aaaggttgga tacttccggc
    31981 cactactttg gcgtttaggc ctgcagccat gatagcccgt atgacccgtt ccaatatgct
    32041 cgagattttg ggtcaggatt atatattgtc agctcgcgcc ttaggattac ccaataaagt
    32101 agtttttgta aaatatgcat taaggaatgc tttaaacact gttgttacag taatcggttt
    32161 gcagcttggc ttgttaatgg gcggtgccgt gatgacagag actgtattca gcctgcccgg
    32221 cataggacgc cttgcagttg aaagcataaa aggaagggat tttccgtcca tccaggtttc
    32281 ggttctatta attgcattga gctttgttat tataaacctt gcagttgata tactgtatgc
    32341 cataataaac ccaagaatta gttacaacta gtcgctgaag ggaggggaac actttggaat
    32401 caaataataa tactgcctca atgccaatga acgatgcgaa aatggagaga gaggccagaa
    32461 taagagaatt aaaacgtttc tggttacaat ttagtaagcg accggtagca gtactgggtt
    32521 tagtgattgt tttaattttt ttagtaatgt gtataactgc gccgctgata gctccctttg
    32581 acccatatga atcagatacc agcaaaagat tgctgaaacc gggagaagaa ggtcatatcc
    32641 tgggaaccga tgagttagga agggaccttt tcagccggat aatattcggc tctcggtatt
    32701 cattgctgat aggggtaggt tcggtaggtc tgggagcttt gataggaatt cctattgggc
    32761 ttattgcagg gtattatgaa ggtaaaattg ggaatgttct aatgaggatt atggatgtat
    32821 ttttggcatt tcctaatata ttattagctc ttattattgt aagtatcctg ggaccaagtt
    32881 tatacaacgc actgatagct ttaagtatat ggtcaattcc ttcttttgcc cgtatagcaa
    32941 gaagcgaagt attagcgcta aagaataatg aattcgttga ggcttccaag gctatgggtt
    33001 caagagacag ttggatagta ctatggcaca tatttccaaa ccttgtgtcc cagctgatag
    33061 tatggattac tctaaatata agcagtgcaa tactgacagg tgccggattg ggatttctcg
    33121 gtcttggtgc acagccgcca tctcctgaat ggggagttat gctggcaaac ggcaggcagt
    33181 atataatgag ggcacatcat ttaactacat atcccggtct ggcgattttg ttccttgcac
    33241 ttgggttaaa tcttctgggc gacgggctga gggatacatt ggatccaagg ttgaaggatt
    33301 aagtatacgg aatgaggtga caaagtgcat actactttaa aaataaaaga tctgtcagtg
    33361 gaataccata ccttcgacgg tattgtaaag gcagttaata atttaaatct agaagtgaag
    33421 aagggcgaaa gcattggttt ggtcggagaa acaggagcag ggaaaacaac aaccgcccta
    33481 tccatactta agctattgcc ttatgcgggt catgtagtaa atggggaaat tgaatttaat
    33541 ggtaaatctt tattaaaggc atcagaacaa gagatgtaca gaatacgggg aaataaaatt
    33601 tcgatggttt tccaggaccc cttaacttcc ctaaatccag tttataatat tggaacccag
    33661 atacgtgatg ttttgacgca tcaccaatcg ctgaacaagg aagaggcaat cgaaaaggcg
    33721 aaagagatga tagaattggt tggaataccg tcaaacaggc taaatgaata tccccatcaa
    33781 tttagtggag gtatgagaca gcgtgtaggt attgcccttg ctttagcctg caatcctgag
    33841 ctattgattg ccgatgagcc cacaacagca ttggacgtta ctatccaagc ccaaattctt
    33901 gctcttatga cagaacttaa gcaaaagttc cagatgtcac ttatcatgat aacccataac
    33961 ctaggaatag tggcagaagt ctgtgacagc gttgctgtta tgtactcggg agaaattata
    34021 gagatcgggt ctgtgaaaaa ggtttttaac aatcctatgc attactatac aaagggactt
    34081 tttggatctg ttccgagccc tgatgtcaaa atcagcagac tcaatcctat caaggggaga
    34141 atgccggatc ccaaaaatct gcccaaagga tgtaagttct atccacgctg caacgctgca
    34201 aggccggaat gcttgaaaat gcctgaacta attaaggtag aagaggatca ttttgtagca
    34261 tgtgttgcag gcaaggaggg agtatagtga gcaaaacgtt tattgagata aaaaatttaa
    34321 agaaatactt caatgtcaag gacgggaaaa aattacatgc tgtagatgat gtttctttca
    34381 acgtagacaa gggtgaaata gtaggattgg taggagaaag cggctgcggt aaatctacca
    34441 ccggcagggt tattctaagg cttttagagg ctactgcagg taatgtattc ttggaagggg
    34501 aagatattat tcaggctaac aagaatagac tgaaacagct gcgcaggaga atgcagatag
    34561 tgttccagga cccgaattct tcactaaacc caagaaaatc ggtgcgtcag attatttcgg
    34621 aaccgctaat agttcaagga atattaaaga gcaaagagat gcaggaaaag gtatataccc
    34681 ttatggaaga gaccggggtg gcgtttgatt tagtgggcaa atatccccac gagctggatg
    34741 gtggggttag gcagcgtgta ggaatagcaa gggccttggc tcttggtccg gaatttattg
    34801 tgtgtgatga gcctgtttca gccctagacg tatcgataca agcccagatg ttaaatcttt
    34861 ttcaggatct taaagaaaag aaggacctga catacctgtt tatttcccac gatttaagcg
    34921 ttgtcagaca tatctccgac cgtatactgg ttatgtacgt aggtcggctt gttgaagagg
    34981 cagaagcaga cgatttgttt ataaacccga tgcatcccta cactaaggct ttgctgtcgg
    35041 caatacctgt acccaagata cgcaaggaga gtaataggat tcttttgaaa ggcgacgttc
    35101 caagcccaat agatccccca ccgggatgca ggtttgcaaa aagatgctgg atggcaaagg
    35161 aagaatgctt tgaaaaagaa cctgagctaa aagaatatag cccgggtcat agagcagcat
    35221 gccacttcgt tgagtaaaac tataccgctg aaaggagcat aattatgaca gagaataaag
    35281 cttatatttt accggaaatg acttgggttg aagcggaaaa ggcctataaa gaggccaaaa
    35341 ttgccataat acccgttggc tcccacgaac agcacgggcc gggcataaag atgaaggtag
    35401 acggtgttca ggcttatgaa ctggcaaaac ttgttgctca gggagtacac ccgcttgcgg
    35461 tggttactcc gccagtaaat atcggtattt cccatcatca tatgaaatac cccggaacag
    35521 taacgctgaa gagttcaaca tttatggcag taattgaaga tatagttaca agcctatata
    35581 ggtttggttt caaaaagttt cttatcataa acgcacatgg cgggaaccag tatgcttgca
    35641 accttgctac aaatgacctt aaagaaaagt tgggagtaga aattgcctat acactttacc
    35701 tggatttggt gccggaattg gtggaaaggg tacctccgga aaagcgcgga catggcgcag
    35761 acccgggttt ggcaccggct ctgtacctgg atgaggacct agtgtatatg gatagactcg
    35821 aaaagggtaa agaacactgg ccttataaaa tgataggacg ggaaaaggtt aaagttaatt
    35881 tcccctatta tactcatgaa atcagtgata atggagtatg gggagatccg gaaggcacca
    35941 gcaaagaatt cgggaaagaa atatgcgatg ctgtagtaga gcgcctaggt gtatttataa
    36001 gggattttgt aaaaaacgca taatatgagg tgatataatg gtaaactcaa ccaaggaaat
    36061 gaaggttgca atacatgacc ttacttggcc ggaagttgat aaaatgagag atcaagtaaa
    36121 aactgtaata attccggtag gctcaacaga gcaacatggc cccaacctct gtatatctac
    36181 cgatgcaaca cgggcttata agttttctct tcgtgttgcc aaggctttgt atcctcacgt
    36241 attagttgca ccgtgcattc atgtcggaat ctccggccat catatggatt ttcccggaac
    36301 tattacatta aggccggaaa caatgattag cacaataacc gattatgtga gcagtttgat
    36361 gaagcacggg ttcaaaaagt tcttcattat taatgcccac gggggtaatg aaggtactct
    36421 tcaggtggcc gttgaaaaca tcagaacaga ttttggtata agaataccct tcgttaatta
    36481 taaggttctt tgtcaagatg ttaccgcaga gattgtggga acaaaaagag tagaccattc
    36541 gggtgaattt gaagtatctg atgctatgtt tttggctccg gaaattgtaa gagaagatgc
    36601 catatgtaaa ggagacgatg ggaacttccc tttagtatat actgatatat atggtaaata
    36661 cagaataaat tatccgttga attggaaaca ggtagctcca agcggagcaa tgggtgaggc
    36721 tcataaagcg actccggaaa aagggaaaca gcttaatgat gctgcactta aaagggctgt
    36781 tgagttctta gagcagttta tggaagatga agattagtgt tgagaaagtt ggcaaaaacg
    36841 tgccgtgtca tgttggcttg gctattgacc atcattaatc ggataaggag gtatatcaat
    36901 gtatattaac caagaacttt gtgttcaatg ccatgcctgt attccttatt gtccaatgaa
    36961 agcaattgaa tacaaggaag aaagcaagcg tgtagagata aatatagacg agtgcgtgga
    37021 atgtgaaatc tgcggtaatg cagacgtgtg tcccacaggt gcactgacca tggaagagat
    37081 gaaatggccc cgtatattaa gaaatcagtt ttctaatccg cttattaccc accctgatac
    37141 cggggtgccg ggacgtggca ccgaagaaat gaagaccaac gacgttacgg gcaggtttaa
    37201 aaggggtatg gtgggaatag ctattgaaat gggacgcccg ggaaccggaa cacgttttta
    37261 tgatgtggaa aaagtagcta tggctgttgc aaaggtagga gtacattttg aaccccataa
    37321 tccggtgaca aagctgatga cggataatac caccggaaaa atgaaggacg atgttctaaa
    37381 cgaaaaggcc ttgtccgcaa ttgttgaatt tgacattccg attgagaaaa ccaaaacggt
    37441 attggaacag ttgaaaactg taacgccttt aattaatact gtgtttagcc ttgaccttgc
    37501 ttgtcgtctt aatgaagacg gcagcaaccc tgcagtttgc ctggctgaag aagcaggtta
    37561 taaagtatct gtaaacggga aaacaaacgt cggtttaggc agacctttgg ctaaggagga
    37621 ataaaaatga cacatacttt acatcgcaaa ggagataaag aaggtttatc caatgattat
    37681 atagtattcg ctatctctgc tcaaactgtt aatgcaaaag gcagcgcacc taaatttaag
    37741 gagtttggag acattgtgtt aaaatataat ccggtaaact acggggacat gaagacggga
    37801 aatgcgtaca atgtaggtgt tgacaaagta catgaaagct ataaggataa ctctattgta
    37861 catgcggtgt tcacagaaga agacattgtt gctaaagtgc ttaaggaact tagggaagcg
    37921 gacttaggac tttcaattgt gctatcggga attgttgaac acacatccga atgctgtcac
    37981 aaaattagta ttgaacctca tactattgag tattcccttg gtatacatgg aaagacagac
    38041 agtctcccgg aagataatgt agttgagatt agtactatgt gtggtcatgg aatggtgtcc
    38101 ttttcgctga ttgaacactt ttgtgaagag atagaaaagg gtaggattac tgcagatgag
    38161 gcggcaagaa agctggctaa gcagtgtcac tgcggagtgt ttaatccaac gagggcggca
    38221 gagatactac gattaatgac tgatatgtaa gatttgtgat atcagtaagg gcagaagata
    38281 aggaatcttc tgcccttatt gttaagttta gacatttaat aagtaaagaa acagaatggc
    38341 gtataataac tttgcctatt gcaaaataga gtccaaaaaa gacgagaaat atagttataa
    38401 atgtgttata tataattata ttggtgaact ttgagtttga aatgtacgtt caaattagct
    38461 attgctaaga ttatttgaag gagagaatac tatgaccata tatggagcgg caattattgc
    38521 cggatgtatg ttcgttggaa gctttatagg tgatgctctg gggggattga tgggcataga
    38581 tgccaatgta ggaggcgttg gattttccat gctttttcta attctaatta ccggttctga
    38641 tttatttggg aaaaaatcgt cggcagcaac ttccgaaggg ctggaatact ggcagagcat
    38701 gtacattccg gtggtagcgg caatgactgc atcacaaaac gttgtacagg ctgtgtcagg
    38761 tggggttatg gctgtcctag cagggcttat tgcagttgct gtaggatttg cacttctgcc
    38821 ggtattgaat aatatgttta agtcatacga agtggagcgg gaaacggagg ttaaaggaga
    38881 atgaacgcgg tttttgaggt tgcccaaaat attatgatca ataacgggct tgtcatgtca
    38941 ttccttttta taggcctgtt aatgtggttt tctactataa tttcaaaata tctgactaaa
    39001 ggaagaatac atagttcggc tatagccata attctagggc ttgttttggc ttatgtgggt
    39061 ggtgtattga ccgggggaaa caagggaatt gcagatattg cagcctttac tggcgtaggc
    39121 gtccttggaa gctcaatgct gcgtgacttt actattgtat caactgcata tggtgcctcg
    39181 atttccgaaa taaaaaaatg tggcattgtc ggtattatat cactattatt gggggtagtg
    39241 tctgctttcc tgataggggc aatttttgcc gtttgctttg gttatgcaga tgcggtaagt
    39301 attactacaa ttgcagccgg aacggttaca tttgtggtgg ggccggtgac cggttcggct
    39361 ttaggtgcta gttctccggt gatcactatt tcaatagctg ccggagtggt aaaatctatt
    39421 gcggttatgg tggtaacacc atttgcagca aagagaattg ggttgactac accgaaagct
    39481 gctatgatat acggcggttt gatgggcact accagtggag tagcggcggg attggcggca
    39541 accgatacta agcttgtccc ctatggcgca atgacagcaa cgttctatac cggtcttggg
    39601 tgtttgcttt gccctactgt acttttttgg ttaacgcggc ttatgtttta aaaggatatc
    39661 cctgtttata tggatggtgc aaggatattt aatgatgcag tagcgttaaa taagacgatg
    39721 catatccact tattcctgct ccacaactct taattcaata ctgctttcat aatgttaata
    39781 aatttccttg caggcttgga aaggtaatga ttctttttat aggcggctat taggttacga
    39841 gaggaattga cattgtctaa ataataatat ataggatgat tcgctagatt tccgtatttg
    39901 acaaagctgt gaggagtaaa tgacactcct aatcctgaaa gtgtcaaatt aaaaattgtg
    39961 tgaatattgg ctgtaattaa aaatggattt ggttcaaatt ttgatagcct gcaaagttca
    40021 actgattttt tgtataggtg ctgggaagtg ttgacaagta aaaaaggttc atctttaaaa
    40081 taactcagaa gttctatagg cattttttta gaatcaaaat tagcatttac aatatcatca
    40141 tacgaaagtt gatagctttt tagcttgttg tttattggat tagattttga taccgttagg
    40201 agtattattt cacgagctat tactttataa ttgaaaatat ttttattcag ataagattct
    40261 gttgaaacga aaatatcgat tgatacttct tgtgcataat ttgataattc ctgcattgtt
    40321 cctgttatca gatgaaattt tacttttggc atactggttg taaaggcttt taaaatgccg
    40381 ggcaatatgg tagaacaacg ataaggagtc attccgactg taagagaact gtttttaaaa
    40441 tcagcgatat ctgaagtttt attttccaga tcgtattgca gtgttctaat tttatatgca
    40501 gtatcaataa atatacgacc tgcttcagtt atttcaatag gatttgtact gcgattaaac
    40561 aatgaaacat ccaattcttt ttcaactttt gttatgcatt ggcttaaaga gggctgtgaa
    40621 atacaaagct ttttggcagc tttggtgaaa ttcctttctt cagccaaagc caaaacgtaa
    40681 tcgtagtagt aatttttcat atctactcac attccttcct cttaatactc aatattttta
    40741 gtgtaatttt actcgcttgt tgcttagtac ctataaatcg cctataagaa ccatgcagaa
    40801 ttcattccgg ttatgctcga tcttcatttt gtttataatg tgatataggt tttacctatg
    40861 atagattgag ttattatatt agacaatagc attatatagt gttataaaaa ttatagggca
    40921 tatttattaa caaatcaatt atttcagtag ctgaatcttt aaacattaaa ttagtaatgg
    40981 aagtgttact tattagagag aaagactgga aatttctttc ataattctgt cattatttga
    41041 aagtaaagac ttccgactat tggcatatta aaaaataagg ggattaatag ttcaccatat
    41101 taaaaaatta cgaggaggaa gtaaaatgaa aaagctagta tctttattaa tggtagtgat
    41161 actcatagta gcactgtcag gatgttccgg cactacagga ggtccggctg aagaagcaaa
    41221 ggaacctgct gaaaaaagca gtaaataccc ttcaaaagct attgagattg ttgtccctta
    41281 tgccgcaggt ggtggagtag acatgaatac acgtgctatt cagcctttcc tggaaaaaga
    41341 acttgataca acggtggtcg taatgaacaa acccggcgga ggcggcgttc taggcgtaac
    41401 atacgccatt atgtcagaac cggatgggaa taggttgctc cttactaacc taattgctat
    41461 gtgttctgcc agtgcactga atacgctgca agtagactta ctagaaggtg tggaatacat
    41521 agggggtact gtagttgacc ccggagttat agcagtttca aaaaacagta aattcaatag
    41581 tttagacgaa atcattgaag aactaaaaaa gaatcccggc agtttatctt ttggtgtaac
    41641 aggagaaact agtctggaca atttaatatg taagaacctt gaggttataa caggtgcaaa
    41701 gtttaatacg gtttcatttg atggcggttc agaggctatg gctgcccttc taggcggaca
    41761 tattgatatc atggggggaa cactttctga aatgctgact tatcataaat ccggcgaagt
    41821 gaagtcattg gcagtaggtc atggagagag aatcgattca atatctgatg ttcccacatt
    41881 tgatgaatta ggttacaaaa tggctgtaac tgcagcaaaa cgtgccataa tgatgccggc
    41941 aggtgcagat gaagagattg taaaggtttt gcaagatgcc gttaaagcag ctgcttccag
    42001 tgaggaattt gcgaaagaat gcgaatcttt aggtttgatg gctagttatt ttacacctga
    42061 agaatgtgtt aagacagtaa ccgaagtatc tgagttcctt aatggattgt atggaaaaaa
    42121 atagagtttg tgtgaataga atacacaaat agaatttgtc atttaattta ctgtgttgat
    42181 tcttaatagt tgattctcaa cgatgaaaga atcaacacag taaaaaaagt atagttttga
    42241 ttcgagccat attttctgat ccgttaaagt cagcaataat tataattatt aaggcatata
    42301 aaagtatatt taagaatcat taatggcttg actaacaaat aagttttgga ggtataattc
    42361 cccttgcgca ttcagaatat ggagatgtta caaaatcatg ggaataaggt tggcagggtt
    42421 catgttgcac agatgcttga agccgggatg aacatggtgg attcttatat cggagctagc
    42481 aggctaatat cccgtaagga taactggctt attttcgatg gaaaagaatt tgagatggac
    42541 aatgacccgc gaagtggaac tgctaagtat gatctggaca aatttgacag agtaattgta
    42601 attggcgcag ccaaaggagt tcaaagatgt gcgatattgt tggaagaggt tctgggagaa
    42661 tacctaaccg gcgggcatgt cataggtaaa cacggtgatg atattatatg caaaaaaata
    42721 ggtgtcaccc tggcaggtca ccctattccg gaccagatgt gtgtagaagg ctgtaaaaag
    42781 attcacgaat ggattaagga tataaacgaa aaagatctta ttattacaat tgcaggtagc
    42841 ggagtatctt cattaatgac gtggccgatc gcaggattca ctattgatga tcttcgaaac
    42901 ctgacccata tgatgcaaat tgaaaaaggt gctttgacaa aagatttaaa cgcgatacga
    42961 aatcatttgg ataggtttaa gggtggcaaa attttacgtt atatgaaaaa ggctacaatt
    43021 gttaaccttg caacaaatga tttgggggga gcaagctgga gttcgcctgg tatccgagaa
    43081 acatatgagg aactgatgaa aggaaaccga ttccttccga ctattgctga cagcacaaca
    43141 tttcagcagg ccgtagaggc atttcataaa tacgatgctt ggaatcgtat tccgaaacat
    43201 atacaaaaat tttttacaac agcagatcct aatgaagaaa ctgtaaaaat ggatgaatat
    43261 gaaaaaatga attcgcgctt gtttaaattg acatctagaa taaacacaat atatcctgca
    43321 gttataaaaa aggcagaaca atttggctac aatgcttata ttttatctga tacaataacg
    43381 gcggaggcgg ttgaagcagg caaagtcatc agctctatag cattaaacat ccaaaacatg
    43441 aaccagcctt tgaagaagcc gtgtgttttg atttcctcag gtgaactaat tgttactgtc
    43501 ggtaaagaga atggtgttgg agggcgaaac caagaattct gtactgcagc ggcattaaga
    43561 attgcaggaa gttcaaaaat agtaatagga tcagcagaca cagacggaac tgacggaccg
    43621 ggaggtttta ttgtggatgg tgtccggaca tgtcttgcag gggcaatcgt agatggttat
    43681 acttgccagg aagctgcgga gaagaatatt aatctcgaag cttcattgaa aagacatgat
    43741 acatcatcag atttatggga tcttggctgt ggaattaaat caacccaaag cataagcgca
    43801 ttggatttac gtattgttgc tattatggaa tagaaaatcc aaattatgag agaagagatg
    43861 aatatgagta gaattgagga tattagagca tttgaaatgc ttaatgccag aggtaaaccg
    43921 acaatttcgg tggaattgac aacttcaaat ggcatcgctg ttgaagcgac aacccctacc
    43981 ggaacttcaa aaggaaaata tgaagcctat gaactatatg atggcgagaa acgatatcaa
    44041 ggacgagggg taaaaaaagc agtagaaaac gtaaacggaa taatacgcaa gacgcttata
    44101 ggaatggatg ttagcaatca gtccgcaatt gatcaatgta tgattgaatt ggacgggacg
    44161 aagaataaaa gtagattggg gggcaatgca attcttccgg tttcggtagc ggtctgtaaa
    44221 gccggtgcag ctgaagcagg tctgccgtta tatcgttata tcgggggatt atcagcaaaa
    44281 agaattccaa atatgatatc aacggttatt accggtggtg aatattccct ttccggattg
    44341 gaattcgagg actacatatt agtttcagat cggaaagaaa agagtgtgga agatggtgtt
    44401 gaaatgattt cctccctgcg caggcgtttg gaggaagtac taagggcaaa gtacggggcg
    44461 ttccctgata cgacaggggc tctggcaccg ccgttgtcat catcaaaaga ggcttttgaa
    44521 gtaatgttgg gtgtgattca agaacttgga tatgacaatt attttactct gggtttggat
    44581 gttgcagcaa atgagctatt tgatgaaagc aacggcagtt atcgattaat ggatcgtata
    44641 gtatccccgg aggaattatt ggaatattat tgctccctgg tatcagaata tccaataacc
    44701 tatatcgaag acggttttga gcaaaatgac tttaaacatt ttgcccaact aaaaaaagct
    44761 gttccacaga tacaaattgt cggtgacgat ttattcgcaa caaatattga gcggctcaaa
    44821 gaaggaataa acaatgacag tgcgaatacg ctactcttaa aaatgaatca gatcgctacg
    44881 gtcagtgaaa cgattaagac atgcaactat gcgcatcaga acgggtatga tgttacagca
    44941 tcattacgtt caggagaaac tattgattca tttgttgcgg atcttgctgt tggtctgggt
    45001 actcatcaga ttaaacttgg ttcgcctgta agaggtgagc gaaatgctaa attcaatcgt
    45061 ttaatgcgta ttaattatga acttttatca gtgggattat aagatgtcaa taagaagcat
    45121 ttgtttggaa ggttgcaata gaatcttgct taaatggaaa taaaaattgt aaggagtaaa
    45181 tgataatgag tttagtccaa atattctcta ttattatggt aattgcaggc gcattgggca
    45241 tttatggagc tttgcagctt gatatagtct acaacaacat tgtaggtccg gctgttatgc
    45301 cgttaatata ctctattgcc ttattagcat tagtggcggt gtactttgcg aagggcagaa
    45361 atgcaccaaa aatagaatgg aatatgatgc taaaaccgcc taaatcagac gcatggattt
    45421 tttatatttt aaatatcgtg atgtttatat tactatattt attgggaact atacccagta
    45481 ttctggtatt tagcgttgga acgagcttat gtctaaaacg gatgaatctt gttaagacaa
    45541 ttgttttcag tgcattatgg gtggcagcat tatattttat atttgttgta tatctggggg
    45601 tacaattttc gacaggcata ttaattaaat agtattctat gaggtgatta aatggcagaa
    45661 acattatctt atttaatgtt aggatttcag gaagtacttg taccaactac attgctaatg
    45721 acgtttattg gagtagtaat gggcacagtt gttggtgtat tgccgggttt ggggccttca
    45781 gcagcattat cggtactgtt accgatggtt tatggcaaag aagcaattcc ttcattggct
    45841 atgcttgcag gtatttatta tggcgctatg tttgggggaa tgataacttc aatttcaata
    45901 aatgttcccg gggaaagcgc atcggtggtt accagttttg atggttatcc cttggctaag
    45961 caggggaaag ccggtatggc tatggggatt tctgcaatct cttcattttg cggcggaaca
    46021 tttggaataa ttcttttaac gattatggga gctgcattgg caaagtttgc gcttgccttt
    46081 ggaccgccgg agtattttgc tatatatctt tttacatttg tagcaattat gacaatagga
    46141 agcggcggaa aaataataga atcagctata gctctgatat ttggcttatt agtttcctgt
    46201 ataggtttgg atgttatgac aggtaccgca cggcttactt ttggttcggt gaacttgctt
    46261 gcgggagttg actttctacc tgcagttgtt ggcttgttcg gactttcaga gatagtatta
    46321 agtctttctg agggtgagaa gatagaaata gataaaaaga aaggaaatta taaacaatat
    46381 tcttttaaga atgtgtttcc taaattgcat gattttattc actcccttcc ggcgattata
    46441 cgcggcggag tggtaggatt tatagttggc gtactgccgg gagcaggtgc aactattgct
    46501 acttttttga cttataatat tgaaaagcgt gtttctaaag atcctgatag ttttggaaaa
    46561 ggaaatatcc gtggtgttgc agtagcggaa gctgcgaaca atggcgcggc atccggcgca
    46621 tttgtccctc tgctgacttt aggtattccg gggtcagcca caactgccat tcttattggg
    46681 gcgtttataa tggttggaat aacaccggga ccggctctct ttagcgaaca tcccgatgta
    46741 gcctggggtt taatttccag catgtatgtc ggaaatgtta tgttgataat aataacgacg
    46801 gcatttattc cattttttat ctgggtatta ggtatttcgc agaagacctt acctattatt
    46861 gttgccagct tgtgtgttgt tggaacatat tccttgaaca attcagtttt tgatgtatta
    46921 ttaatgttgg ttttcactgt aataggcttg tttatgaaaa aacttgaatt ccctgtatca
    46981 ccggttatta ttgctattgt actgggtgga gatttggagt ttgccttccg ccagtcattg
    47041 cagctgtttc aagggaatat atttatgtca tttacaagac caatttgtgt agttttgtat
    47101 cttttgagcc tgttattaat cgctttacca gttgtgggag gaataaaaaa acgacttgca
    47161 gcaaaatccc aggttagcta aaggaatttt ataattaaga aaaacaatgt tgaaagaaaa
    47221 aatcttttta gcttcttagt tttaaagaag aatggggtga tatcttgtga gtgtcgtatc
    47281 aaatttggtc agtgatgtac gattacctcg tatggtaaag gcaaggcaat actttgacaa
    47341 tactgaaata gaaaatgtac ctaaagaatt agaaagacaa tttttaagac cggagatata
    47401 cggcaccata cacccgggta tgagtattgc aattacctgc ggcagcagag gaattgataa
    47461 ttatccatta ataatcaaag aaattgttca attctgcaag gataaaggtg caaaaccttt
    47521 tattattccg gcaatgggga gtcatggcgg agccgtttca gagggacagc gtcttatatg
    47581 cgaaactaac ggggttacag aagagtattg cggttgtcct ataaaagcaa ctatgtcggt
    47641 gacaagaatt ggcgttacag aagacggttc ttttgatgta tgtattgata gttatgcggc
    47701 tgaggccgat ggaatcatct tagtaaatcg cattaaaaat catactgctt tgagaggtga
    47761 ttatgaaagc ggattgatga aaatgatggc aataggactg ggaaaacagt atggagcatc
    47821 cttatgccat cgtgccggtt acgaaagaat ggcagagtta gttccaatga ttggaaaaac
    47881 agtattaaat aatgcatcgg tactttttgg agtcggtatc gttgacaatg cctatgataa
    47941 aaactataag atcgctgttt tgactaagga tgagattccg attgtagaaa aggaattgct
    48001 gaaagaagcg aagagattaa tgcctcgcct gctaccggga agtgcagatg tgttggttgt
    48061 ggacagaatc ggtaaaaata tcagtggttc cggtgcagat cccggcatta caggaagatt
    48121 aacctataag tattccggag ttgaatcttt tcaggcagga aaagttgctg tattggatat
    48181 tactgatgag acacatggaa acattcatgg tgtagggtat gtagatgtta taaatcgaag
    48241 ggtttttgaa aaagctgatt tagataagac gtatccaaat tcaataacat caacaactct
    48301 tatgtctgac aaaattccgg ttatgatgaa gaatgaccgg gatactataa aatgtgctat
    48361 aaaaacttgc caaccggcgg acccggaaaa cataagactt atacgcataa cggatactct
    48421 gcatatatct gaaattttga tatctgaatg tatggttgat tccgtgcgcc acatcccaaa
    48481 tttggagatt actgatgaaa ttatagattg ggaattcaac gaagaaggta atctgtggta
    48541 gaagatgact gtataattaa atcgcaatat tatataaaga ttttaggggg tttacctatg
    48601 tatattaata agggactatg tataaagtgc tgtgcctgta ttccatattg tccaatgcag
    48661 gcgatcgaat acaattctga aaagaagcaa gttgaaatta atgcaaatga atgtgtggaa
    48721 tgtgaaatat gtaaaaatgc aaatgtgtgt cccaaagatg cattaattat ggaagaatta
    48781 aaatggcctc gtattttgag gagtcagttt tcaaatcctt tggttactca ccctgataca
    48841 ggggtgccgg gacgtggtac tgaagaaatg aagacgaatg atgtaaccgg cagattcaaa
    48901 aaaggtatgg taggaatggc tatcgagatg ggtcgcccag gaacaggaac ccgtttcagc
    48961 gatgtagaaa aggtagcgat ggctgttgct gaggcaggtg tgcattttga accgtacaat
    49021 cccgtaacaa ttctaatgac cgacaaagaa accggaaaat tgaaagatga tatcctaaat
    49081 gagaaagttc tgtctgcaat tattgaattt gatatgccga ttaataaaac caaaccggtg
    49141 ctggagaaac taaaatctgt tattccgtta atagatactg tgtttagcct tgatttggct
    49201 tatcgcttaa cagagggtga cagcaattta gcagtaaaaa tagccaatga ggcaggcttt
    49261 actgtatcaa tcaatggcaa gactaatgtt ggattaggca gaccattagc gaaggaggaa
    49321 taaaaaatga cacatacttt acatagaaat ggaaattttg aaagcctgtc aaatgattat
    49381 ataatttttg ctattactgc acaaactgtc aatgccaaag gcagtgcccg aaaatttaaa
    49441 gaatttgagg acattgtatt gaagtataac cccataaact atggagatat gaaaaccggg
    49501 aatatgttca atatagacat aagcaagata caggaaggtt atcgtgataa ttctatagtg
    49561 catgcagtat tctgcgatga agatactgta gcaaaagtac tgaatgagct aaaagaagca
    49621 gatcttggaa tctctatagt agtatcagga attttggacc gtgtttcaga atgttgccat
    49681 gaaaaaggga tcaagcccca tactattgaa cattctcttg gtattcacgg gagggtagat
    49741 tacttgccta atgataacgt tttggaaata agcactatgt gtggtcatgg aatggtatct
    49801 ttttcactta ttgagtacct gagcgagcaa atactaaaag acagaattac cgtggaagag
    49861 gctgcaaaaa agctggctaa gcaatgccat tgcggggttt ttaaccctgc cagggcagaa
    49921 tccatattga gagctatgac taaatagtat gggtcttaat aggcattttg aaatgtaaca
    49981 aggttttatt ttatgaagag aattcataag ggattcgaat ccttgagggc aatttgcact
    50041 aaggaaacga tcatatgcga gtattttaag agtattgtgg gaacccggca cggtagtctc
    50101 tattgttaca gaaagggagg ttcctttctt aagaataaga aactcttgat tcgggcagca
    50161 aaatatatta caataatttc attgtttgga ttcataaagg tagaggtgca gaatgagcaa
    50221 gtttagcttc ggcataattg aatatctggc ttatgctcac gcaattacag gtgattttgg
    50281 agtacaaggg agaagtgtac ccaaaatcga atattatgta aactaataag ccaaaacttt
    50341 actgataaaa atttatatct attgcccctg agtaaggttt ttacttaggg gttttttgcg
    50401 tttaaggaga agattattat gaattatgat attcaattaa aaaataacat taacaagaac
    50461 tatatattta cattgcttca gaatattgac ctgaccaggg gaatatggat gatatacctt
    50521 gcaggtaaag gtatgagcct cacacaatta ggccttctgg agatgatatt ccacataacc
    50581 tcttttctga tggaggttcc caccggagct gtagctgata tttttggaag gaagataagc
    50641 aggatactgg gaagggtgtt gtcattgatc agtgttgtga tactgcttgc tgccgacggc
    50701 ttcctgtggt ttgcaatatc ctttgtgttc actgccctat ccttcaatct tgagtcagga
    50761 gccggggaag ctcttatata tgattcactg aaagaaatcg gtgaagagga cagatatatg
    50821 aagatatccg gaaggaagga ggttttttac caggttgcgg gggtgatctc attcctggcc
    50881 ggtggttata tggctgccaa gagctataat atagcttttg caatcacaat tatcatagga
    50941 gcggctgctg tattgcagtc cttcagtttc aaagagccgg ccgtcgacag aaaaaaagaa
    51001 gaacaaaagg tgaaaaacat atttttgaat cagcttaaag aaagcttgag agtagtaaaa
    51061 agcaatccca ggatagtgtc tctcatagtt ttcacagaaa taatacttac tttttgcacc
    51121 tgtatattct tctatttgca aaactttatg aagggagagg gctataacga ggcaataata
    51181 ggggttgtat acgctgcttc gtttgttgct gcggctttga ccgcatcctg ggtccacagg
    51241 atagagagag tgataaagga acagggaata ctgcttataa taccttttgt cacagtggcg
    51301 tgcttatggg gcatagcact tagcccatac cactttgttt tctttatact gcttacagtt
    51361 acggaagaga tagtgtatat agccatgagt gactatataa acaagatgat acccagtgag
    51421 aaccgtgcta ctatactctc tttttcaagc atggtattca gcttcttcat gatcacggtt
    51481 ttcccacttg taggccttct tggagacagg tattcattaa tttatgcaat caagtgtctt
    51541 ggggggctgg gtattgtatt tgtgataatc aacagtgtat cgctgctgaa tccgggcagg
    51601 aaaatccgga aataggattc tgtttctgcc tatggagaaa actgtttcct ttgcaggagt
    51661 attatgagat gataatagtg aataaatttg cattggcggt gataaattga agctggacag
    51721 actggtttcc attattgtgc tgctgctcag gaaagaaagg gtacaggcca gggagcttgc
    51781 agagatattc gaggtatctg tgagaaccat cctgagagac atagaggcaa taaatcttgc
    51841 aggaattcct atagtcactt accagggagc aaacggagga ataggtattg ccgaaggcta
    51901 ccgccttgac aaaagtgtgc ttacggagaa tgacatgtcc accatcatat ctacgttaag
    51961 aggtattgac ggaacaatac ctgacagccg gcacggtata ctgatggaaa agctccggaa
    52021 cgctttgccg tcatcacagc ttgaggctct ggatgccaag gtacagcagc ttattattga
    52081 tttatccccc tggggcccta atgaactgtt gaagtatagt gtcacatgca taagaaaagc
    52141 aatagagaat cgcaatgaaa tcgagtttga atatatcgat tcgacaggca aaaggacaaa
    52201 tcgcagagtt gagccctaca ccttggtgct gaaggggcag aaatggtatc tgtatgcatg
    52261 gtgtcataca aggcaggatt tcaggctttt caagctttcc aggatgagag ggcttgaggt
    52321 gacgacagca gtctaccagc ccaaaaaggt atctttggaa aggcttgatt gggaggatcc
    52381 ctggagtact tccgaaagta tggttttcct ccggctggtg tttgaaaaag agatggaaag
    52441 cattgttaca gattggttcg gggacaatgt cgaaaagctg gaagacggga gactgctggc
    52501 aaacgccgtt ctgcctgaaa ataactggtt gtatggattt atcctgagct tcggcgcagg
    52561 agcggaggta atagagcctg ctcatattcg gaaaatactc gcagaaattt caaaacagat
    52621 atatgaaaaa tattccatgg aaacatgaca tacaggtgtc atgtatgcag tgttataatc
    52681 aagaaaaagt caaggaggaa ataataatgg attataacat cgagttgaaa gaacaagggc
    52741 ttcagcccgt attatccgtc agaaaaacaa catctgtagg aaatcttccc cgggagatcg
    52801 ggaaggcata cggaagcatt atgcagtacc tgcaagagct gggggaacag ccggcggaag
    52861 cgccatatac ggcatattat aacctggata tggagcatct ggatgtagag atgggttttc
    52921 ctgtttcaaa acagcttgca ggaaagggag aaatcaaagc aggttcaatt ccggcaggaa
    52981 aatacgcgga atgtgtgtac aaagggcctt atgcggaaat ggcgccggta tatgaagcaa
    53041 tgaacaaatg gatagccgaa aagggcttgg aagctgtcgg aacatcctat gaagtctatt
    53101 acaactcacc tgcagagatg cctgaaagcg agcttttgac aaagataatg ctgcctgtaa
    53161 gatagagaaa tatttggagg tgttgttatg aagtacgatt ggaaaaaaca ggataaagag
    53221 ctgtatttgc ctaagaataa gccggaaatt gtaatggtgc ctcctatgtg gtttttcatg
    53281 cttgacggca ggggtaaccc gaataatgag gattttgcca aggcagtagg tgtattgtat
    53341 tctcttgcct atgcagtgaa gatgatgccc aagaagggtg tgaccccgga agggtactat
    53401 gagtactctg tttttcccct ggagggaata tgggaccttg cccctgaggc aagggaactg
    53461 gaggcattgg acaaggacag tctaatatat accataatga taaggcagcc tgactttgtc
    53521 acggaagaaa ttgcacagcg ggctttagaa gccgcagcaa aaaagacaaa gcatccgctg
    53581 ctttcaaaag tcaggtttgg atgcatcgaa gagggattgt gcgtacagat gcttcacata
    53641 ggccactacg acagtgagcc ggcttccttt gccgtaatgg aggaattctg cagggccaat
    53701 aacctgaata ggatatccaa ggttcacagg gaaatatacc tttccgatgc gcggaagact
    53761 cccgcagaaa agcttcagac cgttctgcgg ttcaaagtgg agaaaatctg aggaagcttt
    53821 aattccttaa ataaaggagg ctgttatatg tcagaatatg atctcatact ggacggaagc
    53881 catacgcctg aatatgatga aatagttgaa tatatcaata tgcctgcacg ggatttatgg
    53941 aggcagatca atggttttat ccagcagaga tataaatcct taccca
//
LOCUS       DAOQUR010000013        53225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_689_length_53225_cov_12.225183, whole genome shotgun sequence.
ACCESSION   DAOQUR010000013 DAOQUR010000000
VERSION     DAOQUR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 53225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53225
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_689_length_53225_cov_12.225183"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1844)
                     /locus_tag="PKI50_04450"
     CDS             complement(81..1844)
                     /locus_tag="PKI50_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT02998.1"
                     /translation="MKEGVTDMAKFNNIKLSNKMSLLLTIPIIAIVITTYISVTNIGT
                     VSEVLVKKLYNEINQSTALMLNADRDFYQALADQMNMQKSTNQEELQRYRESYNENAK
                     QVVDRVHEAYGILLRQKSVYEKFRHQVSNKTVFELIEAFNRDYNRWYGLFNASTNVVS
                     DEEQYINAFNSARESMNQIEEVLEVYGAEIVAQNNKTVLQTQRYVVVITALAVILSLL
                     LGIIIITGISKRTRKTLELIKKTANLDLENSAEYDKFLKEKDEFGLIINAESEVRKEL
                     VNIIKEVSNNASTINKIVDLTNQRMSRLGEEIDGVSATTEELSAGMEETAASVQEMNA
                     ASNEIERASENIAENAKEGSLEAEKISKTANELNKSFTVSQESAYRIFEEVKISLEEA
                     LERSKAAEQISILADSILEITSQTNLLALNAAIEAARAGEAGKGFAVVADEIRKLAEN
                     SKNMVTEIQNITQTVLSSVNDLSSSSKSLLDFMNSDVVKDYGMMLQATKQYKKDAEFV
                     NGLVENLSATAEMLLVSIQSTVKALDEVSAATNEGAEGTSNIAQQTSNIVENAGEVIK
                     NIANAKEVSAKLNQMIAKFKI"
     gene            complement(2280..2378)
                     /locus_tag="PKI50_04455"
                     /pseudo
     CDS             complement(2280..2378)
                     /locus_tag="PKI50_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003546024.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
     gene            2500..3876
                     /locus_tag="PKI50_04460"
     CDS             2500..3876
                     /locus_tag="PKI50_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT02999.1"
                     /translation="MKKDKEVIFFILILVLLLSVSNVYADEEPIIPATPVLTPDITDQ
                     TDKTVTVTISNWDTADTKEYKIDNGPWQEYTEPVALTTNATIYARGSASSGTVSDIAS
                     LKITNIRRLLSKTEVSVQASLTVIIVIYDINNNVVGYGSGFIVSSDGQVVTNYHIIDM
                     APRIDVITTNNETYAVAGVSAYDKKLDLAVLKLEKVKDLPAVILGDSDSLQLGDDIVA
                     IGYPLGLPVTVSFGNVSSLNTPGVLYRTGQKDIQITAPISSGSSGGPLINMYGEVVGI
                     NYSSIENAQNINYSIPINDLKPMLAPASIKKLPDIIKKVYPSMNYDQLAKYLYFNYLD
                     KISGDYTFSFTDIEIRDSGNQPDLVDVYLDLNSLKYAEILLAELEGNKKEVEKWIDEI
                     YSKVKAINPRKNVYVHVALYWSFATRPQGYTNQEAYYNADTKRWVIYKKKVKYGYKNN
                     KSDITWYK"
     gene            3895..5244
                     /locus_tag="PKI50_04465"
     CDS             3895..5244
                     /locus_tag="PKI50_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT03000.1"
                     /translation="MKKARFILPLIIMMMFLFSLDIFAEELLSAEDYYNKGLAFFEQS
                     NYSEAINNYKKAIELDPTYIQAYLDMSDACFYQRKYEEAIANYDAVIELEPANAYAYY
                     SKGVMLYFLFKYDEAIKMYDEAIKIDPDYGDAYQSKGNSLFSLERYSEAMECYDKALK
                     LNPFDADIIEALGDTYYLQELYEKAIEYYDLAIYYNTSDKFVYIYKGCSLYHLSKYDE
                     AIKALELGLEKLPDDAVANYYMSLSLVKTGNTKDYCPYLKKAVSLNKLYLKRAPSEEA
                     FKNLLQDEAFIEIIYDAEEEIHDIFENSDILESDALAEIHFLNMKTSGNAKEDAKALK
                     AERDFLNNFYNGNIYNSASAKKCHISSNSSHITDFIKSGRLIKGQALVGLKITHNISK
                     ENILYIGKQDGVLKAVIFEIAGEIKMDGQSAAINDYIIFIYNNGIWEYFGNLEPKEL"
     gene            complement(5536..5949)
                     /locus_tag="PKI50_04470"
     CDS             complement(5536..5949)
                     /locus_tag="PKI50_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT03001.1"
                     /translation="MGFICTPGDKVLLKVNLIGSKSSDSAAVTHSEFVRAVARILKQR
                     GCEVWIGDSSGGAIGGIAPTAKSFRTAGYEKAAMEEGAVKNVFGCIPGLRKARYHKMA
                     PNPESFGEILADIHQNAEFKLPKRYKSGKSETIKY"
     gene            complement(5910..6038)
                     /locus_tag="PKI50_04475"
     CDS             complement(5910..6038)
                     /locus_tag="PKI50_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03002.1"
                     /translation="MAKVVIQECREYELNGLMIKINDGIERLGGWDSFVHPGIRFY"
     gene            complement(6058..6618)
                     /locus_tag="PKI50_04480"
     CDS             complement(6058..6618)
                     /locus_tag="PKI50_04480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNT03003.1"
                     /translation="MSVSMFHRLEGRNIYFKPLNIIDAEAIHSYASDENVKRFIGWPL
                     MKSLEETRSYIEDMLKRESAGTHLYASVALKSTREIIGTVMIFNFDREANHAEIGYVF
                     HSKHWGKGHGTESVALMCSFAFEILALHKIHARVVDANISSARILEKNGFLLEGRLKD
                     HYFIDGRYYDSLIYGKFEADEKTLSY"
     gene            complement(6661..7836)
                     /locus_tag="PKI50_04485"
     CDS             complement(6661..7836)
                     /locus_tag="PKI50_04485"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNT03004.1"
                     /translation="MMNKLEIMGRDIRKDLQLFAQELVRIKSTSGNEETIIRFIASKM
                     EALGYDEVIIDGFGNVVGRVGSGEKAVMFDSHVDTVEVKGAELWKVPPFSGEIIDGWL
                     WGRGSVDMKSGAAASIYAAAIAKSQGLLEGKRVYVSCTIFEEDCDGENLKHMFKELQF
                     KPDFFVTCEPSGNIITTGHKGKAQIVIKTQGISAHGSAPEKGVNAIYEMAEIINRVEK
                     TNLELMTKAAPKGTMVMSRISSTGASLNAVPSECEVYLDRRMVVGETEEIIKNEIEKI
                     IKGKNATWEIGTLHRKTWTGMDITYEPFHLAWEISLDHKLTKAFIDSYKEVFGTEPEK
                     YDFWDFSTNAVATVSMGIPTIGFGPGEYKLAHMTNERCEISQIIDSCSIYTNVITKL"
     gene            complement(7936..8511)
                     /gene="msrA"
                     /locus_tag="PKI50_04490"
     CDS             complement(7936..8511)
                     /gene="msrA"
                     /locus_tag="PKI50_04490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNT03005.1"
                     /translation="MATAHITGTDKKLQTAIFAGGCFWCMVSPFDVLEGIIEVKSGYS
                     GGHKENPTYKDVKSQTSGHYEVIKIIYDPEIIGYDKLLQAYWRQVDPTDDGGQFQDRG
                     ESYRTAIFYTNEEQRQMAEASRKAMDESGRFPGPIVTPIIPAAVFYEAEEYHQDFYKK
                     SPKEYKEDRKISGRDEFIKKCWGDEYYDIYK"
     gene            complement(8590..9234)
                     /locus_tag="PKI50_04495"
                     /pseudo
     CDS             complement(8590..9234)
                     /locus_tag="PKI50_04495"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(9330..9785)
                     /locus_tag="PKI50_04500"
     CDS             complement(9330..9785)
                     /locus_tag="PKI50_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNT03006.1"
                     /translation="MTVWCPALIRRRKDYTGGYDKDGNLTDDPNAIFDGGEVLPSGYW
                     RGSGFSIVIDMVVSALSVGKSTYDMGMNKNDKGVSQVFIAINPIAYAGENHADAYIEN
                     MSQYIKTSAKKAGVTDILYPGEKAARERIENMRENIPVNKEMWETLLKL"
     gene            complement(9757..11268)
                     /locus_tag="PKI50_04505"
     CDS             complement(9757..11268)
                     /locus_tag="PKI50_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT03007.1"
                     /translation="MKKIIIVGAGIAGLSAGIYARQSGMDVTIFESHTIPGGASTSWR
                     RKGYLFEGGMHWLTGSSPKVPLNKVWRELGALNDDVPVYNKDPFLTVEFEGRTAFLYR
                     DVEKLRQHLLELAPEDRNEIDKLCRDIKRFSKVSMPIRDIKNVKVKKKTSVPLSDFIK
                     MLPAFSRMSFYANQTAGEFAMRFKSPILRALIRCIIGEEYSAIGLIFTIATLASGDGG
                     YPEGGSLAMASRMAKYFESLGGKINYKSRVTQVSVKDGISDGVIINGEHFPADAIIVT
                     QDTLVAIDSLFDPPINEPWAYKMRQSIKPTLNTFICLGIEADLSDLPETISFMPHKPL
                     LCGGAEVEFISINNYSGYEGYAPNGCTAVTSIISGDTYDYWKACREHGTYEEEKRKLA
                     ENFIEILAERFPEIREKVALWDVATPLTYERYLSSYKGSWMSITGKGEKMNGYPSKSE
                     KIQNVYFAGQRLSPPGGLPVAVETGRKAVQYLCRDTGTIFQANINDGMVPGTY"
     gene            complement(11272..11844)
                     /locus_tag="PKI50_04510"
     CDS             complement(11272..11844)
                     /locus_tag="PKI50_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT03008.1"
                     /translation="MVTRKEQKYKRRNEILEAGLDLFIRRGYASTKISDIANQVGMST
                     GLLFHYFESKEKLYVELIKNGLAGPMSIMTDSGMEPIVFFKTTADWILGSLKTDTVFA
                     KYFVLMSQAYYNESAPQSVKDMLKGFDIYTPTSHLIKKGQANGSIKEGEPYALAIAYW
                     CAIQGIAEQMAMTPEYPCPEGQWIVDILRR"
     gene            complement(12063..12542)
                     /gene="rlmH"
                     /locus_tag="PKI50_04515"
     CDS             complement(12063..12542)
                     /gene="rlmH"
                     /locus_tag="PKI50_04515"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNT03009.1"
                     /translation="MNISIITVGKLKERYLKEAVEEYSTRLSKYCKLDIMEVPDEKAP
                     ENLSPAQELTVMQKEGQGILRHIKEDTYVIALAIQGRQISSEGLADFINDLGIKGRSN
                     IAFIIGSSLGLSEEVMKRADFKLSFSPMTFPHQLMRVMLLEQVYRGFRIIKGEPYHK"
     gene            complement(12542..13756)
                     /locus_tag="PKI50_04520"
     CDS             complement(12542..13756)
                     /locus_tag="PKI50_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT03010.1"
                     /translation="MDNFDENSYPEIQGNKTAEENNTSAPTAYRNEPIRYKRPSGFLF
                     LTTALVGAVIGGLIVAAIVPSYLYDRMLSVQQGGIAPSGQQLVIQPSNEMNVAAAVAQ
                     KVTPAVVRISTVTVERDLFFGKRSYEGVGSGVIIDPKGYILTNAHVVGEHPEELTVFF
                     KDGKELDGTVLWQDSLLDLAVVKVEATGLPSAELGDSDSLIVGEISIAIGNPLGMRFE
                     RTVTQGIVSGLNRSIMVEQGSVMEDLIQTDAAINPGNSGGPLINGRGQVIGINTVKAS
                     AEGLGFAIPINITKPIIRKLIDTGEFIPTYMGITPLDSEMLGYYAINIEIQKGIYIYN
                     IMKGAPAASAGLEAGDVITHINGTEVNTLVKFKSLLYSLNPGDTVSVRYLRSNTEYNS
                     DIKLAEMPEEYR"
     gene            complement(13838..14635)
                     /locus_tag="PKI50_04525"
     CDS             complement(13838..14635)
                     /locus_tag="PKI50_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT03011.1"
                     /translation="MGIRLCSLSSGSSGNCIFVGNDRSGLLVDCGISGKETLKNLHSI
                     GVCSSTIKAVVVTHEHSDHIKSVGIISRKLGIPIYANINTWNSMRALIGTVKPENIRS
                     FKTGEELEIAGIGIKSFNIPHDAADPVGFSFYEGNNKVSIATDLGYFSDTVRENIRGS
                     DMILLESNHDIEMLMTGRYPFFLKRRIMSERGHLSNEAAGNAVNELLQTGVREVLLGH
                     LSQENNFPELAYETVKGILKQNNINVDADIKLDLAPRNGISRFYNID"
     gene            complement(14663..15922)
                     /locus_tag="PKI50_04530"
     CDS             complement(14663..15922)
                     /locus_tag="PKI50_04530"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT03012.1"
                     /translation="MEEKLIIKGNKKLQGEVCISGAKNSAVGIIPAALLSDSGCIIKN
                     LPDIEDVRYLKKLLQQVGAKVNDGNTGELEVISKGMKNYRVNFEIAKKMRASYYFLGA
                     FLGKFKKAEVPFPGGCDIGIRPIDQHIKGLEALGAKIKVEHGIIKAKASELVGAPIYL
                     DVVSVGATINIMLAACKAKGVTTIENAAKEPYIVDIANLLNAMGANIKGAGTDVIKIK
                     GVEHLGSCEHMIIPDMMEAGTYMIAAAATQGDLLVKNVIPTHLESVSAKLREMGIEVD
                     ENGDSIRVTTVARPKAVNIKTIPYPGFPTDLQQPMSVLLSISEGTGIITESIWEGRFK
                     HVDELKRMGANIKVEGRVAIIEGINKLSGSPVHATDLRAGAAMVIAGLTAEGETEIDN
                     LKHIDRGYENMVEKLRNAGADITRVQC"
     gene            complement(16019..16897)
                     /gene="yycI"
                     /locus_tag="PKI50_04535"
     CDS             complement(16019..16897)
                     /gene="yycI"
                     /locus_tag="PKI50_04535"
                     /inference="COORDINATES: protein motif:HMM:NF021186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HNT03013.1"
                     /translation="MDWAKAKTILIVVFTAINIFLGYMVFAAGTGGFGYVDSEKAKQI
                     TDYLAEKDVTVTGRIPGKKADMPSIIVKYKLFSKEDVKGSFFSTGDVPEESASENTVG
                     LKGKNIEITIRNNREFYYSDNGIKPAGATDEKACRKNIREFLSRLNITDNLDTGISED
                     IEGYKRFLFSQSFKGAEIYNSIMEFYVNDSGVHRARIVWFDTIRQAGKKTEVISPLIA
                     LLYVPEHNKKSELPGREVVEIRQGYYFGTGAGEQVDVSEVVEGTAFPVWKIETDRDII
                     YINAYNEKVEGIEKRQ"
     gene            complement(16955..18379)
                     /gene="yycH"
                     /locus_tag="PKI50_04540"
     CDS             complement(16955..18379)
                     /gene="yycH"
                     /locus_tag="PKI50_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HNT03014.1"
                     /translation="MIKLNRERIKSVSLLFLVILSFVLTTRIWFYTSIEGVFIMSGNK
                     SIKPDYNAEYDKSDLLRPHKLIVSFGERSTLLYDNEEYSEAYGRIFKEAKRVMRTAIL
                     VNSKEVVKRIPMEELYKIRNSRGITFVYNLPLEMEAVYKLMNINSYPDTGIRSIDEII
                     VAQSLDRAYLCDSVQGILYEFRTEGIQNNLDYLISNLEKQPTVLSLYLDSIQPEMYGK
                     NSAVPARIGANGFPVLSGQKEVEGSDEIPESIAGLFDDEITSLSIIKNMDGTIVYTNR
                     EGQVVKLYTDGMLEYVNFNLQPEDKTTVGLGGAIDISTEFVSRHFGFPEDCYISDIIR
                     INSMGDDKYIIRYRYAYEGLPVLSASDISSGSIEVEIVGDEVRRYKRIRRTLNHNFEY
                     KQAMNSINVLDILLDKKVEALSGERIKKVNDMYLAYYERFGQNSITYVPVWVAEVTVE
                     RIDKGTVLNQKYIINAETGIILDK"
     gene            complement(18390..18911)
                     /locus_tag="PKI50_04545"
     CDS             complement(18390..18911)
                     /locus_tag="PKI50_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03015.1"
                     /translation="MFRKMLISAIIVTAVFAAGSAVYAQSGTDYTKETAPVFKLLSDN
                     ESKTFDRDYMITGSASEGTVVTIELYWFKSAKSIIVNKKQSTSSENEGDWIFQDSKEY
                     TVGSSGIFAEPVALNLGKNKIVLLISNTEGVTEEITLELVRSLEKDAKEEVNGPSLNK
                     FVEDLSSKVNSTE"
     gene            complement(19003..>19265)
                     /locus_tag="PKI50_04550"
     CDS             complement(19003..>19265)
                     /locus_tag="PKI50_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03016.1"
                     /translation="AKRLKDSVEIRIADSGIGIPKEDLPRLFERFYRVDKARSRSMGG
                     TGLGLSIAKNIVEAHKGSIRIESEYGKGSEVIINFPCEESLL"
     assembly_gap    19266..19365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19366..20930)
                     /locus_tag="PKI50_04555"
     CDS             complement(<19366..20930)
                     /locus_tag="PKI50_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNT03017.1"
                     /translation="MFKSIQWKLVIISFLLVWLAMSIVGVFITEAIKKEQIDSMTDTI
                     ITRGYYLADNLKDKIGGASNIQEIVSNWFVGQGKQVRAVFVYENGGFTASQTNYEGAV
                     DDLDLLGLWIVEEAIDQAQEITWPVDNSKYLMSIAVPIISSNNDVAGAIYISADLFGI
                     EENIMSINKILTSATILALCITALLGNLLSRTFTGPIKEVTSKAERLAKGDFGQTIAV
                     KSRDEVGQLTEMFNYLTVRLKSTLDEMSREKNKVEAILTNMTDGIIAVNAEGVVIHAN
                     PAAYKIFNIREEDLYNRNFDDAAEKLDLGITFNDILNDSDNNYNILSINNLIIKISVV
                     QIINEHNKAEGAMLVLQDVTEQEKLDKMRKEFVANVSHELRTPLTTIRSYTETLLDGA
                     LDNKEYTLNFLKVINSESERMTRLVKDLLQLSKLDYDKMEWNMKSLSILNILRDCVVK
                     MEMAAKQKNQSLSFEAIGELCEINGDKDRIEQVIINIISNAIKYTPENGSIKVTAKRL
                     KDSVEIRIADSGIG"
     gene            complement(20934..21632)
                     /locus_tag="PKI50_04560"
     CDS             complement(20934..21632)
                     /locus_tag="PKI50_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT03018.1"
                     /translation="MENRRILVVDDERPISDIIKFNLEKEGYKVFVAEDGQQAVNMTY
                     EHKPDLIILDIMLPIMDGFTVCKKLRENVSTPIIMLTAKEEEVDKVLGLELGADDYMT
                     KPFSVRELMARVKANIRRANFLNSGLEGSGQVVKSGNLIIDLNKYEVRKDDTVLELTL
                     REFELLRYLALHAEQVFSRETLLEEVWGYEYYGDVRTVDVTVRRLREKIEDDPSNAVY
                     VQTKRGVGYYFRRI"
     gene            complement(22083..22916)
                     /locus_tag="PKI50_04565"
     CDS             complement(22083..22916)
                     /locus_tag="PKI50_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03019.1"
                     /translation="MKKRISKPAIVLILAAVLFFFTGDKGFMAVKMQGYEALKVFAHI
                     KSSEFTPEFNIYETEHFIIKYTDKDEDTVRDIAGIFEKSYKVEGIHYGFYSKDKTIVF
                     VYNDQESMWDYQQSVKGQAVMGLYNLGIIHVLSPKAYLDSMQIYAKDYEADGPILHEY
                     VHRVIDYRSGGNVELWLTEGLALYEEYDVDGTEWAKGFSYERHYTAHELRDDFMELDE
                     TQSYRQSFDIVWSLIEQRGRNSINLMLDELKYGSTLDEAFIRIYGEDLNEYIDSGAWK
                     R"
     gene            complement(23027..23236)
                     /locus_tag="PKI50_04570"
     CDS             complement(23027..23236)
                     /locus_tag="PKI50_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT03020.1"
                     /translation="MALIDFKCIKCGEKFEEIVKLSDRENIKCPKCGSEDIKQVFEGK
                     CCSVGSSKGVGGCSSGSCGGCSGCH"
     gene            23350..24573
                     /locus_tag="PKI50_04575"
     CDS             23350..24573
                     /locus_tag="PKI50_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT03021.1"
                     /translation="MISISLCLKSFKKVDPAVKRYISIKKVKTIPVIITFKRPLDPKT
                     ESALKRKGLRIKYHIPFLNAVTGKISIGNFDSVSSFVEIAKIYFDGTAKLMGGTESGD
                     KALESPEAASVHLSGKGITAAFIDSGVYPHPDLTRPRNRITAFKDYINEINEPYDDNG
                     HGTACIGAAFGASIDGKFKAAAYDCNIVCAKAFNSLGYGCFSDILAAMQWIYSIKEKY
                     NIKVLVLPFGCCCTSRHFDILSLASEALWNNGLFICTCAGNLGPHEASITSPGICGSA
                     FTTGACTVSGSSSRVAPFSGRGPVDGKFDKPDAVMPGYKIATLNADTDYIPGSKSSYQ
                     SKLSGQQYTQLSGTSAAASMTAAAAVLLYQKKPNLPPSDIKSLLKRFCISLNELKTAQ
                     GAGIIEIKKIGEFKF"
     gene            complement(24609..26093)
                     /locus_tag="PKI50_04580"
     CDS             complement(24609..26093)
                     /locus_tag="PKI50_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT03022.1"
                     /translation="MEGSKLKEAAARYWNKISGGIENIKKHIKNIIKKIANNGANSLN
                     KHTYSRKIVNKRRFAIACGLIALLVFASIFVYASGKAYTVKVNGVEICKVKNPKTVES
                     ALNILKQKYQEKADSEISFTSEVTYEKTRASSKEILKDDALVEVLGKNVEFKVQGCSL
                     YADGNIIAILKTKEKAEELLKEIQDRSLAGADRSKLKEIGFAEKVELRDEFIDASEIA
                     DKDEIIDFIIKGTNEIRIHEVKSGESFWSISRKYNMSLLDLEKANPGVNPERIQIGQK
                     INLIVPKPLISVKTVETITFIEKAEFDQKVEFSSSLYKDETSIRVKGIYGEKEVVADV
                     TKVNGIETGRTILSEKMLKEPKTQIIVKGTKEPPPKKGTGTFSYPARGSITSRYGMRW
                     GRRHTGIDIGAPYGSDVKASDGGEVTFVGYQGGYGKLIIIDHGANFVSYYGHLSKYSV
                     KVGQKVYKGQKIGEVGSSGNSTGPHVHFEIRKNGVIQNPANYLK"
     gene            26383..27408
                     /locus_tag="PKI50_04585"
     CDS             26383..27408
                     /locus_tag="PKI50_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HNT03023.1"
                     /translation="MNLFRIKSNNEDLGQTIVENLFISHYMPFAPADYVKVYLLGLKY
                     SQSYVNNMLSTDTVAKTLGITRDEVHAAWRYWSEQDIIKLYPYSPDDAESGFTVEYLS
                     IKEVMLNIKEEKPSAGKYSPERIINARGNQDVRAMFDSFRKLFGRELSPNELFVFLDW
                     MDDYNFPPDVIKMLVEECVSRDRKEMPYLKQVAKNWFDAGIDSVDKAKQYNSRYKEKW
                     QKYNKVLSFLRIGRQPTSAEEEMLQKWFYTFEYSEDIVLKACELTASTLKPSFSYIDR
                     ILTEWHGKGLSTMPEVEAYLVKNPSKAGRNSKPEKRTFNNFANRTYDAKALEEAWLKK
                     SRGELSE"
     gene            27401..28381
                     /locus_tag="PKI50_04590"
     CDS             27401..28381
                     /locus_tag="PKI50_04590"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03024.1"
                     /translation="MNNNQIIQKQYELIRKENMEEIEARKKTLYDKLPRLKEIEDEMV
                     KLSIDITRAILSKSNTAEVLIDKLKNKQLDLKVEKAEILSANKYPIDYLDSIYKCKLC
                     KDTGFVNYQKCKCYKQREIYLNYEYSNLSFILEKENFEQFRWDYYSDDPDENGISPLK
                     NMQEIYKKCIDFVEQFDEHNKNLFFIGSPGLGKTFLCNSIAKDLLDKGRSVIYMTVPD
                     LIDTMRKYKFDFDKEENYAHYLNEIYNCDLLIMDDLGTELSTQFSNQAIYNILNKRIV
                     NMKKMIISTNLSGVDFQSTYSERIVSRIIGNFEACKFVGKDIRLIMKGIS"
     gene            complement(28439..31372)
                     /locus_tag="PKI50_04595"
     CDS             complement(28439..31372)
                     /locus_tag="PKI50_04595"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNT03025.1"
                     /translation="MAGEVLRENPFRDEIYNYLLSIGYKESIKADYDYMNAIDRAKLF
                     EFLENSQKEQLESFKDTYKDSWMERFIKLLNDKINNEGLLAALKGKLEDYQSSTGFSL
                     AFFGSNIESMTYNKELYEKNIFSACREFAYEDKQDSFRVDIAIFLNGIPIIMIELKKQ
                     TTGQKAGFEGTSQFRNTRNPDELIFGFNKRTLVYFAVDEFEAFAATRLNRKNTVFVPF
                     NRGSSDEGAGNPIVEGKHCTNYVWEEILRKNMLLKIIREFMFIDEDGEMIFPRYHQLD
                     AVLKLEADVKQNGIGGRYLIWHSAGSGKTKTIAWLSKRLINIQDIHTVIVISDRTVID
                     SQLGIEIQTLDGKKGVAKWIDTTSAELLKMLRSGGYIVITTLQKFGNILDELKNIPNR
                     NYAIIIDEAHSSTGGKSLSKTAETLDGKSLKDSILIDETYEELEDNQNRILKQGSKIK
                     STKNISYFAFTATPKTETVELFGTRTAIGKKYFHKYSMKQAIQEGFILNPIVCYTIYN
                     EKFELAKKKDDPTEYETAKASAAILNHITSSAEVIDSKTEIIMEDFLIKRINWLDGKA
                     KAMIITPSRKHAVCYKLAVDKYLIKKGYNIKTIAAFTGTIELNGIKYTEENMNPGFKE
                     KDIKEIIKNNDDVRVIIVADKLQTGFDEKKLCILYVDKKLNSAVKAVQTLSRINRICK
                     GKKTFILDFVNKTEEIKAYFEQYYGGELFLPSENETDPNILFTKRDRILNYFVFTIEQ
                     AEYAYSLINDNARHSGELTSIFAKAKEKYNEMDKDRKKLFLAEISKFVKLFYYVSIVY
                     NIWNSDMKRFAVFLDALYNVLYEKSDDTRINPGELVELVEYSVKKASEEEDLLVNVAD
                     YSLERVPTESYVKEESYSLIDEIIYRINARYGKYENAHQELVEIVNTLSSDNEMIINV
                     RDSSPSAYEAEAMDKIGQIFVNGILSAAEERSQFYAQISSDKDVIRSLAKAVIRRIQD
                     ELKAG"
     gene            complement(31388..32659)
                     /locus_tag="PKI50_04600"
     CDS             complement(31388..32659)
                     /locus_tag="PKI50_04600"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNT03026.1"
                     /translation="MHNSQCIIYNGKISINEVKTKYLFKIVNGATPSSSNIEYWDGNI
                     TWITPADMSKCISNIIIDSDRKITEEGLNSCGTSLVPAGSIILSTRAPIGSVCIAGVE
                     LCTNQGCKALVAKDNVNERFYFYYLLISAELLNSLGRGTTFLELSGSDLGNFLLPFPK
                     KAKQDEIANYLDQKCAEIDRVVAAKQKQNELLKEYKQSIIYEAVTKGLDKNVKYKDSG
                     VEWIGEIPEDWEKWKIKYVCDVDKNRLSEDTDPNYLFKYIDISSVTEMGGIGETVEMT
                     FDTSPSRARMIVSKGDTIISTVRTYLKAIAFIEESNKYICSTGFAVLTSKKGLLPRFL
                     FYQAQSEYFIQEVVSRSVGVSYPAITSSALSNISFFVPSLPTQQRIADYLDQKCCEID
                     RIIKSNDDMITKLKEYRQSVIYEAVTGKIEM"
     gene            complement(32659..34383)
                     /locus_tag="PKI50_04605"
     CDS             complement(32659..34383)
                     /locus_tag="PKI50_04605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNT03027.1"
                     /translation="MINQADINAKVNLVTKIANKLRGPYTSEQYGSVIIPMSILRRFD
                     CVLEGQNSKIKLILEKFKSLNVDEKLIEQAVQKECGIPFYNKKCVTFEDLISDSDNIN
                     ANFMEFLTSYSPSIANIIKGLKFKEEVEFMIKKGILFEVVKAFSEVDFHPDTTDQLSM
                     GYIFEEIIRTYKSNAEAGDHYTPREVIELCVELMVAENIDELKKEGKIIRVYDGCCGT
                     GGMLTVTKRKLGELLGSDERVRLFGQDNNPEAYAICCAEMLMLGEDVKNIYEGNTLTE
                     DHLKGEKFDILITNPPFGVEWKTEKAIVEKELNSPNNRFVAGTPRVSDGQLLFLQNLL
                     AKAEKNARIAIILNGSPLFSGDAESGESEIRRYVLENNLLEAIVALPDQMFYNTGIFS
                     YIWILSRNKSEKRDKKVQLINAVDFWEPQQPKSLGNKRKFISPHNRKKIVELYSKFEE
                     NEYSRKFDNIDFGYHKVTIERPLRDETGAPIIKKGKVQADGALRDTENIPLKEDINEY
                     FNREVKPYAPDAWIDHDKIKVGYEIPFTRYFYKYVPPRKSGDILSEIIEIEDDIKGTI
                     ETLLGRRE"
     gene            complement(34380..35495)
                     /locus_tag="PKI50_04610"
     CDS             complement(34380..35495)
                     /locus_tag="PKI50_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT03028.1"
                     /translation="MDKLKPYNDLTPISQLAIEENKPLLKLAEDTRVAIEILNYEINS
                     LPSPSIMIDTLTLQEAKVSSHIENIVTTNDDLYKGIVFDNYTAEAKEVTNYKTALFIG
                     FERLKEKGVLSIGDIEAINEPVNKKTKGIRVNMPEFENSLTRIANKRGNEIEVLYTPP
                     HGKELLQKLLIDMLEYLYDDETYTIHPLIKIALAHYQFECIHPFYDGNGRTGRILNVL
                     MLCQKRYLDYPILFASSYIIKNKNEYYELLQTCRQKDNYTSIIEYMLNSFKVTAEDTL
                     NKVNSIKKLIEKYSDQEFLETLKGQKKVLSQVMELIFKKVYVRIEDIVEMGIHRQTAS
                     AYLKQLVEKGLLQEEKVQRDKVFKNIELLKLFEGEQI"
     gene            complement(36084..37058)
                     /locus_tag="PKI50_04615"
     CDS             complement(36084..37058)
                     /locus_tag="PKI50_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNT03029.1"
                     /translation="MKIVVLDGYTLNPGDLSWDGLEKLGEITVYDRVSYDEKDEDMVA
                     ESVGDAEIVFTNKTPVTRRAMKKMPNLRFIGVLATGYNIVDVVAAKEKGVVVTNIPTY
                     GTDSVGQFAIALLLEMCHHVGAHSDSVLRGEWANNPDWCYWNFPLIELAGKTMGIIGY
                     GRIGQTTGRIAQALGMRVIAADSYKNPALESDTMKYVELDELFAKSDVIALHCPLFES
                     TKGIINRENIAKMKDGVMIINNSRGPLIVEEDLAEALNSGKVAGAAVDVVMTEPIKPD
                     NPLLKAKNCIITPHISWAPKESRKRLMDIAVNNLEKFLAGTPVNVVNP"
     gene            complement(37204..37857)
                     /locus_tag="PKI50_04620"
     CDS             complement(37204..37857)
                     /locus_tag="PKI50_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNT03030.1"
                     /translation="MKKIDLNIKKELPIILIIVLMFAASIYFYPQLPDKIPTHWNFKG
                     EIDGYSGKLTGAFLMPVMNIIMYGLFIFIPAIDPKKENYKLFESTYIYFRYLFHIFFL
                     GIHGLIIASALGYNVDTGRLVTLAVSLLLMLMGNVMGRLKHNYFVGIKTPWTLANEEV
                     WRKTHRLGAILWTAGGLAGVIMSLLRLSMGWAFIAVIAVMTFIPIVYSYVIYSKFKK"
     gene            complement(37844..38143)
                     /locus_tag="PKI50_04625"
     CDS             complement(37844..38143)
                     /locus_tag="PKI50_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HNT03031.1"
                     /translation="MKLSILNMPFKALSDPSRRKIIQMLKGKDMTAGEIADNFQMSKP
                     SISHHLSILKQADLVTDERRGQNIYYSLNTTVFQEVVNWFFNMTDRSGGFENEKD"
     gene            complement(38280..38355)
                     /locus_tag="PKI50_04630"
     tRNA            complement(38280..38355)
                     /locus_tag="PKI50_04630"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38320..38322),aa:Thr,seq:tgt)
     gene            complement(38359..38435)
                     /locus_tag="PKI50_04635"
     tRNA            complement(38359..38435)
                     /locus_tag="PKI50_04635"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38399..38401),aa:Asp,seq:gtc)
     gene            complement(38580..38655)
                     /locus_tag="PKI50_04640"
     tRNA            complement(38580..38655)
                     /locus_tag="PKI50_04640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38620..38622),aa:Val,seq:tac)
     gene            complement(38662..38736)
                     /locus_tag="PKI50_04645"
     tRNA            complement(38662..38736)
                     /locus_tag="PKI50_04645"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38701..38703),aa:Glu,seq:ttc)
     gene            complement(38742..38817)
                     /locus_tag="PKI50_04650"
     tRNA            complement(38742..38817)
                     /locus_tag="PKI50_04650"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38782..38784),aa:Lys,seq:ttt)
     gene            complement(38927..40204)
                     /locus_tag="PKI50_04655"
     CDS             complement(38927..40204)
                     /locus_tag="PKI50_04655"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNT03032.1"
                     /translation="MSSLVIVGAQWGDEGKGKLTDYLAEKADVVVRYQGGNNAGHTVE
                     VDNEVYKLQLIPSGILYADKMCIIGNGVVIDPEAFLNEIRNLESRGISTGNLRISDRA
                     HVLFPYHKKIDELSELKRGSDDLGTTKKGIGPCYADKTERIGIRVCDLLKKEVLAKKL
                     KTNLELKNAYLKKIYDFEGYSYEEMLSKYLDYAEQVRPFVEDTSVLIYDAIKAGKKVL
                     FEGAQGTLLDLDFGTYPYVTSSHPIAGGVCIGAGVGPALIGKVIGVVKAYTTRVGKGP
                     FPTELFDETGEYIRTTGKEFGTVTGRPRRCGWFDAVIVRYAARISGLTGIAITKLDTL
                     SNIPTIKMCVGYRLDGKIIRDFPASLEDLENCEPIYEEFEGWGDIGDITQYNKLPTSV
                     LRYLERIEEICGTKISIISVGPKRNQTIMIDKF"
     gene            complement(40237..41619)
                     /locus_tag="PKI50_04660"
     CDS             complement(40237..41619)
                     /locus_tag="PKI50_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT03033.1"
                     /translation="MKYDVIIVGAGPSGIFAAYELMQYDAGIKVAILEKGNELEKRAC
                     PIDNKKIKSCINCPTCSIMNGFGGAGGMSDGKYNITNNFGGELYKYIGRKEALDLMYY
                     VDEVNCKMGGEGAMLYSTENSSLRTQALRHDLHLLDAKVRHMGTDKNLVIMKNLYEYL
                     MDKVEIIFNNEVSDIEKQGDTFIVSAEKGEYSCSHLILAAGRSGSKWISQICEKLGIK
                     TESNRVDIGVRVEIPASIFEHITSQVYESKIVYRTEKYNDLVRTFCMNPHGKVVTENT
                     NGIVTVNGHSYSDPKHHTENTNFALLVSNRFTEPFKNSNEYGESIAKLSNMLGGGVLV
                     QRFGDLVKGRRSSERRMEKSFLRPTLNATPGDLSLVIPKRQLDDIIEMIYALDKIAPG
                     TANEDTLLYGVEVKFYNSQVEVDNNLETIIKGLYILGDSSGVTHSLSQASASGVYVAR
                     LLGEKYCKHS"
     gene            complement(41993..43447)
                     /locus_tag="PKI50_04665"
     CDS             complement(41993..43447)
                     /locus_tag="PKI50_04665"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HNT03034.1"
                     /translation="MIDIIVNEKDLSFNRLEKEIYAYGCQIAREILKSVLEAMDKRLA
                     EERDKSKYRHRGGRNTSIKTLMGEVEFSRVIYEGKDDEDRKSFIYLLDELLGFDTIGQ
                     ISTNLAEKIVENASITSYRNAADNITELTGQGISHGGVWNVVQALGQKVREEETELAK
                     AAEKNQLCGGKEVPVLFEEADGVYINIQGKDRPKSGKKLEMKVAVAYEGWKEVSKNRF
                     ELVNKVACAGFEDISEFYKIKEAMISREYNVDEIRMRILNGDGAAWIKHGIDDTVHYQ
                     LDPFHKHKAILKSVRDGEQRKTILKLLNSNEINEALAYIAGLANDTEDEKEQKKLKDL
                     YSYFKENRDGLIPYQERGLDIPKPPLGIEYRNLGTMEHHICDIIAQRMKHRKASWSIE
                     GANNLGKLLAVKASKRLNEVVERHSKIVLPEEKTIEIIQILSSSKAPKKDGKGKDGNI
                     HKGQRPFTNYPVTNGRKAIRSMFDMRNFTDLVYR"
     gene            complement(43960..45300)
                     /gene="dnaB"
                     /locus_tag="PKI50_04670"
     CDS             complement(43960..45300)
                     /gene="dnaB"
                     /locus_tag="PKI50_04670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNT03035.1"
                     /translation="MSEMIQRIPPNNIEAEQSVLGAMLLDKEAISTATEYISGDDFYR
                     EAHKEIFEAIVDIYNRGEPVDLITLTEKLKTRNTLEAVGGITFLTNLMDAVPTTHNVK
                     YYAKIVEDKSLLRKLIKSSNEIIQKSYQAEEDIGEIIDDAEKGIFNISLKRSTQGFVH
                     LKNILNANFDRIEQLYLNKGKITGVPTGFHDLDNKLSGLQKSDLILIAARPSMGKSSF
                     MMNIVQHAAVREKTTTAIFSLEMSKEQLTQRLLCSEALIDAHRLRIGDINEDEWVKLA
                     RAMGPLSEAPIYIDDTPSISITEMRAKCRRLKLEHDLGLIVIDYLQLMQGKGYAESRQ
                     QEISEISRSLKALAREINVPVVALSQLSRAPEMRADHRPVLSDLRESGAIEQDADVVM
                     FLYRDEYYHPDTEKKNIGEINISKQRNGPTGTIELIWLGQFTKFVNKEKHITAI"
     gene            complement(45319..47151)
                     /gene="lonC"
                     /locus_tag="PKI50_04675"
     CDS             complement(45319..47151)
                     /gene="lonC"
                     /locus_tag="PKI50_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HNT03036.1"
                     /translation="MSHNHSSIRSKDVSIDVLVDFTKKLYSSDKTRKLDNVTDNKEKI
                     YILHEIVSGKIENKRIEENKLEFLIEDIKDKLSDAAAKRYIEEGIKREVIKRMETREI
                     EYLNEMKLQVIKKHAGLENASTLKKYADLELMKKVRLTKSIMDVMRPQRQDEIIGQEN
                     AVSALISKIASPFPQHVILYGPPGVGKTTTARLALEIAKSRKHTPFSKTAKFVEVDAT
                     TLRWDPKESINPLLGSVHDPIFQGANKELAEMGIPEPKPGLVTEAHGGVLFIDEIGEL
                     DVMLQNKLLKVLEDKKVRFDSSYYDVNNENIPRYIKLLFEEGAPADFILIGATTRQPE
                     EINPALRSRCAEVFFNPLSSSDIARIVLNAADKLGARISAKGAELISSYTYEARKAVN
                     ILADCYSSMINEREDKDIEISVDCIKEVLSKGRYVPLNKKKAEGIREIGRINGLGVNG
                     FIGNVIELEAVCFAREDEAGTIRFNETAGSMTKDSLFNALSVIRKITDKNINKYDIHV
                     NCVGGGKVDGPSAGAAITCLLYSAILERPARQDTCITGEISIRGRIKAVGGIVEKISG
                     AKRAGFKNVIIPEENKEEANGLDGINIIPAGNIQEVIGFMFDEQ"
     gene            complement(47155..47610)
                     /gene="rplI"
                     /locus_tag="PKI50_04680"
     CDS             complement(47155..47610)
                     /gene="rplI"
                     /locus_tag="PKI50_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNT03037.1"
                     /translation="MKVVLLQDVKNLGKKEQLVNVSDGYARNYLFPRKLAAEATSGKL
                     KELEEKKNSEIIKKDKEKQAAKELADKLEKLEVNFKVKAGENGKLFGSITSKDVADAL
                     KSQHKTEIDKKKIVLHDAIKALGTYKVEIKVYPGISTLINVKVEEQRGV"
     gene            complement(47611..49608)
                     /locus_tag="PKI50_04685"
     CDS             complement(47611..49608)
                     /locus_tag="PKI50_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNT03038.1"
                     /translation="MKIRKFSDIWGPDVSISLWILAIAVVVIGYYNVFLGLAGALLLG
                     YLVYHNLHAATRKKEELRLYIENLSENVDTATKNAILNLPFPLVIIDDQGVINWYNML
                     FSSIFQGEYILDKKLSSYLSGVDISRILKNKMERFKNINIKDRYYDIYCNLININESG
                     EEKNIIIMYFVDKTDFISLRNRYNDEKPIAAILQVDNIEEVIKETEEALRPALVAEVD
                     RRVNAWAALNNASIQKYSNGEYMIYFSHKQLMSMEEKRFDILDSIREINSGNKIPITL
                     SIGVGVNGKNHAELQKFCTSAMDIALGRGGDQAVVKDNEKLSFYGGKTKAVEKRTRVK
                     ARVIAYALRQLIEQSEDVLIMGHEMPDLDCMGSALGIYRCCKGLNKEVKIVLGGVNAG
                     IANLWSELQEDEEYADVFVSPQDALQICGANTLLVVVDVHRPAFTVQKQLVEKAEKIA
                     IIDHHRRGSEFIENAVITYIEPYASSTSELVTELLQYMFEKPKIKQIEAEALLAGIFI
                     DTKNFSFQTGVRTFEAASYLRRMGADTTLTKQLFQDDMATFLTRAEVVKGAQMFKSNI
                     AISTIPVNAKNPALVAAQAADELLNIKGVNASFVLSCIEGDIFISGRSLGDINVQVVL
                     EKLGGGGHLTVAGAQLPGLTMEKAMSLLKKAIEEYLKEGER"
     gene            complement(49605..50588)
                     /locus_tag="PKI50_04690"
     CDS             complement(49605..50588)
                     /locus_tag="PKI50_04690"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HNT03039.1"
                     /translation="MERMQTRALVEGAIFAGVTALLGILYYYTQYLGIIAMVWPVPVI
                     IVGYRNGIKASILSALSAGLVVSLVTQPLVGVGLLIGFGVPGVLMGHMINKRVNPYAV
                     ILVCGVVLSVSMAGEFLLSLKVAGIDGLEFLSNLDSAFREQLETSLNIYRQFGAAEEK
                     LQEFKDYFSQAIELMKYVIPSSLLISGVFFSFIDYKLTRLILKRIGLILPDIDEFSKW
                     RLTEPYSYILLGTAVLAGVASYFKVPGFNAVVLNISTILSLIFSIVGISAVVYFARVY
                     GDRYGIPKALRRIITVFVILTFIQFIAFIGILDMAFDFRRLKSKNRIGGVR"
     gene            complement(50598..50918)
                     /locus_tag="PKI50_04695"
     CDS             complement(50598..50918)
                     /locus_tag="PKI50_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HNT03040.1"
                     /translation="MLNQDTDITKNIKMIEFLKSELLTTVASLYQNLLKGTKVGQGVI
                     QDILANLVLVTYLLGKRLGVPYSMIDTKILEKIRLGMLEEHETEKWYGDLTDLGDYLK
                     TKKG"
     gene            complement(51267..51482)
                     /gene="rpsR"
                     /locus_tag="PKI50_04700"
     CDS             complement(51267..51482)
                     /gene="rpsR"
                     /locus_tag="PKI50_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNT03041.1"
                     /translation="MRKPKKKVCNFCIDKVEHIDYKDVNKLRRFISERGKIIPRRISG
                     NCAKHQRQLTIAIKRARNIAFLPFTTD"
     gene            complement(51566..51976)
                     /locus_tag="PKI50_04705"
     CDS             complement(51566..51976)
                     /locus_tag="PKI50_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNT03042.1"
                     /translation="MLNKVALVGRLTRDPEIRYTADNQTPIAKFTIAVDRTYKREGQP
                     TADFIPIIVFGKSADNCGKFIKKGRLVAVSGRLQTRNWDDQDGKRHYATEVIADEVDF
                     LDRGSDARGQGNEGRSDSDYGDFHLAEEEDELPF"
     gene            complement(52018..52302)
                     /gene="rpsF"
                     /locus_tag="PKI50_04710"
     CDS             complement(52018..52302)
                     /gene="rpsF"
                     /locus_tag="PKI50_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNT03043.1"
                     /translation="MNRYETIYIIRPTVDEEGIKALQEKFKAIVEENGEIEAIDDWGK
                     RKLAYLIDDIGEGYYVYMKYKAAQVVPKELDRVFGITEEILRHMIIKIEE"
     gene            complement(52399..52617)
                     /locus_tag="PKI50_04715"
     CDS             complement(52399..52617)
                     /locus_tag="PKI50_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNT03044.1"
                     /translation="MYYPKQFNIGDIVETKKQHPCGDNHWEVIRVGADFKIKCLKCGR
                     IVMLPRPKFEKSVKKVIKSAAPEENNID"
     gene            complement(52629..53207)
                     /gene="yedF"
                     /locus_tag="PKI50_04720"
     CDS             complement(52629..53207)
                     /gene="yedF"
                     /locus_tag="PKI50_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HNT03045.1"
                     /translation="MKEIDARGLVCPQPVILTKKALEGMTEGEVVTIVDNITAKENVS
                     RLAENLSCKYEISDESGCYYIKIIKAAGAESTETKEDSTVIVITSDKLGQGAEELGKV
                     LIKSYTYALTEATPLPKAVMFLNSGVKLTAEGSEVAENIKKLENSGVEIISCGTCLDF
                     YQLKDKLQVGIVGNMYSIIEKMNSAGKVINIG"
BASE COUNT        14184 a        12508 c         8933 g        17500 t
ORIGIN      
        1 atacatttga tacttaacat gcagaaatca aataaaactc ccttaatggg agtttatttg
       61 tatgcagact tacatactgc ctatatttta aacttggcta tcatctgatt aagcttggcc
      121 gatacttctt ttgcatttgc gatgttttta ataacttcac cggcgttttc cacaatgttg
      181 gatgtctgct gtgctatatt gctcgtcccc tcggcgcctt catttgtcgc tgcagatact
      241 tcatcaagcg ctttaaccgt gctttgaatt gagaccagca gcatttccgc cgtagcgctc
      301 agattttcta ccagcccgtt tacaaattca gcatcctttt tatattgttt tgttgcttga
      361 agcatcattc catagtcctt tacaacatcg ctattcataa agtccagcaa gcttttggaa
      421 ctggatgata aatcattgac tgaactcaat acagtctgtg taatgttttg aatctctgta
      481 accatattct tggagttttc tgccagcttc cttatttcat cagctacaac tgcaaagcct
      541 ttccctgctt ctccggctct tgcagcttca attgcggcat ttaaggcaag caggtttgtc
      601 tgtgaagtta tttccagaat agaatcggct aatatactaa tctgttcggc agcctttgat
      661 ctttccaatg cttcttccag gcttattttt acttcttcga atattctgta agcactttcc
      721 tgggaaacag taaagctctt attcaattca ttggcagtct tgctgatttt ttccgcttcc
      781 aaggatcctt ctttggcgtt ttcggcaata ttctccgagg ccctttcgat ttcattggag
      841 gctgcattca tttcctgaac tgaggcggcg gtttcttcca ttcctgccga tagctcttcc
      901 gttgtagcag atacaccgtc aatttcttct cccaggcgtg acattctttg attggtcaaa
      961 tcaactattt tgtttatggt tgatgcatta ttgcttactt ctttgattat atttactaat
     1021 tcttttcgca cctcagattc ggcattgatt attaacccga attcatcttt ttccttcagg
     1081 aatttatcat attctgccga attttctaaa tcaagattgg cagtcttttt tatcagctcc
     1141 agagtttttc tggttctttt acttatgccg gttattataa taattccaag cagcagagaa
     1201 agtataacgg ccaaagctgt tatcacgaca acatatctct gtgtttgcaa gacagtttta
     1261 ttattctgag caacaatttc agccccatat acctctaata cctcttctat ctggttcata
     1321 ctctctcttg ccgaattaaa ggcatttata tactgctcct catctgaaac gacattggta
     1381 cttgcattga acaatccata ccatctatta tagtccctat taaaagcctc aataagctca
     1441 aatactgttt tatttgaaac ctgatgcctg aatttttcat atacactctt ttgcctaagt
     1501 agaattccat acgcttcatg tacacggtca acaacctgct ttgcattctc attgtatgat
     1561 tctctatatc tttgcagctc ctcctgattt gttgatttct gcatgttcat ctggtctgca
     1621 agggcttgat agaagtccct gtcggcattt aacattaaag ccgtgctttg gttgatctca
     1681 ttatacagtt ttttgaccaa tacttctgaa acagttccta tattagtaac tgaaatataa
     1741 gtagtaatta caatagcaat aataggtatt gtgagtaata gagacatttt gttgcttaat
     1801 tttatgttgt tgaattttgc catatctgtt acaccttctt tcatttcaga tttcctcata
     1861 tatttcgaca cttcttttat cattccttca atttcttaga aaaattggaa tgattaaacc
     1921 gactgtaatc aattccttga gaaacgccaa tataatacta gtccgcaaaa gcaaatccat
     1981 actgatgtca agccccctcc tgcatgctct gaacatgaac cttacgaatt tggaataatt
     2041 tacataaaaa acaatcccgg caataattcc cctcctgcga atatatatat tgagatataa
     2101 tattttgaat gtgggcggaa acatgagtat cagtgaattc aatagcaagg taaatcttat
     2161 tattactttt gtcaataaag tgaacgctgc ggctgtccct gatgacatta aaaaaattat
     2221 ggtgaaggct tatgcaagta ctctaagcat caatctttct gacaggatgg cagaacgcat
     2281 tatttccgca aaatctcatc tatcgctttt ccctttgcca actcatcgat cagcttatcc
     2341 aaatagcgaa tttcccgcat agtaggttat ttgcggataa ttctgtcagc ttccgctgca
     2401 acgcaccata ataataagct ttatttcaaa gcctatatct ggtaaaataa tattgtaatg
     2461 gtaataatgg aaaaatatta taaacggaag ggctttgtaa tgaaaaaaga taaagaagtt
     2521 atatttttta ttcttattct ggtactgtta ttatctgtat caaacgttta cgcagatgaa
     2581 gaaccaatca tcccggcaac cccagttttg acaccggata tcacagatca gactgataaa
     2641 actgttacgg tcacaatcag caactgggat actgctgata ccaaagaata caaaatcgat
     2701 aacggcccct ggcaggaata taccgagccg gtggccttaa caacaaatgc aactatttat
     2761 gcaagaggca gtgcttcttc cgggacagta tccgatattg ccagtctgaa aataacaaac
     2821 ataagaagac ttttaagcaa aacagaggta tcagtgcaag cttccttgac agtaataatc
     2881 gtaatttatg acattaataa taatgtagtc ggctatggaa gcggttttat agtttcttcc
     2941 gacggacaag ttgtcactaa ctaccatata attgatatgg ctccaagaat agatgtcatt
     3001 acaacgaata atgaaaccta tgctgtcgcc ggggtctcgg catacgataa aaaacttgac
     3061 cttgcagttt tgaagctgga aaaagttaag gatttgccgg cagtaatact tggagattct
     3121 gatagcctcc agttgggaga tgatattgta gctataggat atccgttagg cctgccggtc
     3181 actgtatcat tcggaaatgt aagcagcctc aatactccgg gtgtgttata ccgaaccggg
     3241 caaaaggata tccagattac ggcacccata tccagtggaa gcagcggcgg gccgttaatt
     3301 aatatgtatg gtgaggttgt aggaataaac tattcctcca ttgaaaatgc acagaatatt
     3361 aattattcaa tacctataaa cgatttgaaa ccaatgcttg ctcctgcgag tataaagaaa
     3421 ttgcctgata tcatcaagaa agtatatcca tccatgaatt atgaccagct ggctaaatat
     3481 ttatatttta actaccttga taaaataagc ggtgattaca ccttcagctt cacagatatt
     3541 gagataagag actccggtaa tcaacctgat cttgtggatg tatacctgga cctgaacagc
     3601 ctgaagtatg cggaaatttt actggccgaa cttgaaggaa acaagaagga agttgagaaa
     3661 tggattgatg aaatttattc aaaggtaaaa gccattaatc ctcgtaagaa tgtttatgtc
     3721 catgtggcgc tttattggtc gttcgctaca cggccccaag gctataccaa ccaggaagcc
     3781 tattacaatg cagacacaaa aagatgggta atatacaaaa aaaaggtgaa atacggctat
     3841 aaaaacaata aatctgatat tacctggtat aaataacata agggagattt ggatatgaaa
     3901 aaagctcgtt tcattctacc gcttatcata atgatgatgt ttttgttttc attagacata
     3961 tttgcagaag aattgctctc cgccgaagac tactataata agggacttgc cttcttcgag
     4021 cagtcaaatt attcagaagc tatcaataat tataaaaagg caattgaact ggatcctaca
     4081 tatatacagg cgtacttgga catgagtgat gcctgttttt accagagaaa atatgaagaa
     4141 gctattgcaa actatgatgc cgtgatagaa ttggagcctg ccaacgcata tgcttattac
     4201 agcaagggtg tgatgttata ttttctcttc aaatatgatg aagcaataaa aatgtatgat
     4261 gaagcaataa aaattgaccc ggattatgga gatgcctatc aaagtaaagg gaattcctta
     4321 ttctccctcg aaagatattc cgaagccatg gaatgctatg acaaagcttt aaagctcaat
     4381 ccatttgatg ctgatatcat tgaagcctta ggcgatactt attatcttca agaattgtat
     4441 gaaaaagcca tcgagtatta tgatttagct atctattaca atacctccga taaatttgtc
     4501 tacatataca aagggtgttc cctataccat ttatccaaat acgacgaagc cataaaggca
     4561 cttgaactgg ggttggagaa actccctgac gatgccgttg caaattacta tatgagctta
     4621 tcgttggtta aaacgggaaa taccaaagac tactgtccat atttaaaaaa agctgtttcc
     4681 ctgaataaat tatatttaaa aagagcacca tctgaagaag ccttcaaaaa tctgttgcaa
     4741 gacgaagcct ttatagagat aatctatgat gctgaagaag aaattcacga tatattcgaa
     4801 aactcagaca tactggagag cgacgcactt gcagaaatac attttcttaa tatgaagaca
     4861 tccgggaatg caaaagaaga tgcaaaagca ctaaaagctg aacgagattt tttaaacaat
     4921 ttttataatg gtaacatata taattcggca agtgccaaaa aatgccacat tagtagtaat
     4981 tcatcccaca taaccgattt tataaaatca ggcagattaa taaaaggcca ggcccttgta
     5041 ggtctgaaaa tcacccacaa tatttccaag gaaaatatac tttacatagg taaacaggat
     5101 ggtgtattga aagctgtaat ctttgaaatt gcaggtgaaa taaaaatgga tggccaatcc
     5161 gctgctatta acgattatat tatatttata tataacaatg gtatttggga atacttcggg
     5221 aatctggaac ctaaagaatt gtaagtgcta atatgtgaat tcaggcacgt caattggact
     5281 gcctgttttt ttatctgtat ttaccgcggt taaggccgat taatatgccg gcaaggaagt
     5341 tttccttctt tagctctact gctttataca tacataattc atgacagcac atacattcaa
     5401 tacaaacctt ataatcaatt tgcttagtat ccctgttaat cgcttttaca gggcagctct
     5461 ccacacatac attacaattc ctgcactttt tcctgttgac cttttgtgcg gttttaaaga
     5521 aatcaataac cttaatcagt actttattgt ttcgctttta ccgcttttat agcgctttgg
     5581 cagtttaaat tctgcattct gatgaatatc cgccaggatt tcaccaaagc tctccggatt
     5641 gggagccatt ttatgatatc ttgcttttcg caggccggga atgcatccga atacattttt
     5701 gacagcccct tcttccattg ccgctttctc gtagcctgct gtcctaaagc tctttgcagt
     5761 aggtgctatg ccgccaatgg ctccccccga actatctccg atccacactt cgcatcctcg
     5821 ctgctttaaa atccttgcca ctgcccggac aaattcagaa tgagttacag ccgctgaatc
     5881 agatgatttt gagcctatga gatttacctt cagtagaacc ttatccccgg gtgtacaaat
     5941 gaatcccatc ctcccaatct ttctatcccg tcgttaattt ttatcatcaa accgtttaat
     6001 tcatattccc tgcattcttg aataactact ttagccaact taatccagct cctttactta
     6061 gtatgacaat gttttttcat cagcttcaaa ctttccgtaa atcagactgt cataatacct
     6121 tccgtctatg aaataatgat cttttaaccg cccttccagc agaaatccgt tcttttccaa
     6181 tatccgggca gaactaatat tagcatcgac cacacgggca tggatcttgt gaagagcaag
     6241 tatttcaaat gcaaagctgc acattaaagc aacactttcc gtaccatgcc ccttacccca
     6301 atgtttgctg tgaaaaacat aaccgatttc cgcatgattg gcttcccggt caaaattaaa
     6361 aatcatcacc gtgcctatga tttcccgggt tgacttaaga gctacggatg catataaatg
     6421 tgttcctgcc gactcacgct tcagcatgtc ttcaatgtag ctgcgggttt cctccaatga
     6481 tttcatcaat ggccagccaa taaatcgttt gacattctca tccgaggcat agctgtgtat
     6541 cgcttcggca tcaattatat tcagtggttt aaaatatata ttcctgcctt ctaacctatg
     6601 aaacatgctt acactcacgt tattccccct catgctttga ataagtgccg gaaaacatag
     6661 ttacaattta gttatcacat ttgtatagat gctgcaggaa tcgataatct ggcttatttc
     6721 acagcgctcg tttgtcatgt gagccagctt gtattccccg gggccgaagc ctatggtagg
     6781 aatgcccata ctaactgtcg caacagcatt tgtactaaag tcccagaaat catatttctc
     6841 aggttcagta ccaaaaacct ccttgtagga atcaataaat gcttttgtaa gtttgtgatc
     6901 caaggagatt tcccatgcca aatgaaaggg ctcgtaagtt atgtccattc cggtccatgt
     6961 tttcctatgc aaagttccta tttcccaagt ggcattcttt ccctttatga tcttttcaat
     7021 ttcattcttg attatttcct ctgtttcacc gacaaccata cgccggtcca aataaacctc
     7081 acactcagaa ggcactgcat tgagcgatgc accggtactg gagattcttg acatcaccat
     7141 tgttcctttc ggagctgcct tagtcataag ctccaggttt gttttttcta ctctgtttat
     7201 gatttccgcc atttcataaa tggcattcac tcctttttcc ggtgccgagc catgagcaga
     7261 aatcccctgt gtctttatta caatctgtgc tttccctttg tggccggtag ttattatatt
     7321 tcccgacggt tcacaagtta caaagaaatc cggcttgaac tgaagttctt taaacatatg
     7381 cttcaagttc tccccatcac agtcctcttc aaaaatagta caggaaacat aaacccgttt
     7441 tccttccagc agtccctggc ttttggcaat tgccgccgca tatatggacg ccgctgcgcc
     7501 ggatttcata tccactgagc ctcttcccca gagccagcca tctatgattt cgccgctgaa
     7561 tggaggcacc ttccacagct ccgcaccctt tacctccact gtgtccacat gggaatcgaa
     7621 catgactgcc ttttcaccgc ttccaacacg gccaaccaca ttgccgaatc catcgattat
     7681 aacttcatca tagcctaagg cttccatttt acttgctata aatctaatta tcgtttcctc
     7741 attccccgac gtacttttga tacggaccag ctcctgagca aataactgca gatccttcct
     7801 gatgtctctc cccataatct ccagtttatt catcatattc ctcccaaaaa accagattcc
     7861 gaactaccat tcatacctga ttatacacga tttggaggta aaagcataag tgccaggcat
     7921 catatttgcc gggctttatt tataaatatc atagtattca tcgccccaac acttctttat
     7981 aaattcatct ctgccggaaa tctttctgtc ctccttatat tcctttgggc tttttttata
     8041 aaaatcctgg tggtattctt ccgcttcata aaacacagca gccggaatta ttggagtaac
     8101 tatggggccc gggaaccgtc cggactcatc catggctttc cttgatgctt cagccatttg
     8161 cctctgctct tcatttgtat agaaaatagc tgttctatag gattcacccc gatcctgaaa
     8221 ctgccccccg tcgtctgtag ggtcaacttg tctccaatat gcctgaagaa gcttatcata
     8281 accaattatc tcagggtcat aaataatctt gataacttca tagtgtccgg aggtttgtga
     8341 tttcacatcc ttatatgtgg ggttttcctt gtgtcctccc gaatatcccg attttacttc
     8401 aatgattccc tctaaaacat caaagggaga aaccatgcac cagaaacacc cgcctgcaaa
     8461 tatggcagtc tgaagttttt tgtcagtgcc tgtgatatgg gccgtggcca tatattcatt
     8521 tccttttggc actgtataat tatccctggt aaactctatg gatttcccct gatgcaatgc
     8581 cgcaacccac agatgataca ttgcaatgcc aatatcaatt ctgttgaact tgctgtacag
     8641 tgcttccttc aatatattag gcagcctgcg gcttattatt atgcaatccg tactgcctga
     8701 aaaataccac ggctgtgtgt ttgaagcaga tggcgccagc ctgacggctt caagaagttc
     8761 ctcagcaccg tttacagaag atatttcagg caatgacttg cgcttgaact ctgttgtgct
     8821 ttgcctgtga aggggctcat cagcatatcc aaatgccata gctattacaa actccaggcc
     8881 attccttgaa gcagactcct tctgaggcag gcccaggccc agccagcaaa cccccatttc
     8941 ctttgcagac agatacagat ccaactgctg cagcataaag cctgcatttt gcaaatatcc
     9001 attctttgtt tcagaatata tcgcaatata atgcggtgat cttataggca gcagtaactt
     9061 aatatccttc tccgacagta tggctgtttc tgttttgata tcctcatata aaggcgcaag
     9121 cctttccata aacttctgca attccataag cctggagctt tccagcggtt cttttctata
     9181 tttgcgtatg gactttctct taaatatcgt gttgtagaaa tcagcgttat tcatagttct
     9241 ctcctttatg ctctgataca tgagagcctg gtattattga tatatcttgg aacaatgtgc
     9301 ctgtcccatt gtcacttaaa aggcgctagt tataacttca gtaatgtttc ccacatttcc
     9361 ttgtttacgg ggatattttc tctcatattt tctatccttt ctctggcagc cttttctccc
     9421 gggtaaagga tatcagtaac acctgccttc tttgctgatg tcttaatata ctgagacata
     9481 ttttctatgt aagcatcagc atggttctca cctgcatatg ctatgggatt tattgcaata
     9541 aacacttgtg aaacgccttt atcgtttttg ttcatcccca tatcataggt agatttgcca
     9601 actgatagtg ctgaaacaac catgtcaatg actatggaaa atccagatcc cctccaataa
     9661 cctgaaggaa gaacctctcc tccatcaaat atggcattgg ggtcgtccgt aagatttcca
     9721 tctttatcat agccgcctgt gtagtctttc cttctcctaa taagtgccgg gcaccatacc
     9781 gtcatttata ttcgcttgaa aaattgttcc cgtatccctg caaagatatt gtacagcctt
     9841 ccttccggtt tcaacagcta ctggcaaacc acctggagga gaaagccgct gacctgcaaa
     9901 atatacattt tgtatttttt cggactttga tggataacca ttcatctttt ctcccttgcc
     9961 cgttattgac atccatgagc ctttatagga actcagatac ctttcatatg tcagtggtgt
    10021 tgcaacatcc cacaaagcca ccttttctct gatttccggg aacctttcag ccagtatttc
    10081 aataaaattc tcagccagct tcctcttttc ttcttcatag gtgccatgtt ccctgcatgc
    10141 cttccaataa tcataggtat ccccagaaat tattgaggta actgcggtgc aaccattcgg
    10201 tgcatatcct tcgtagccag aatagttatt gatagaaata aattctactt cagctccacc
    10261 acataaaagc ggtttatgag gcatgaagct aattgtttca ggtagatctg acaaatctgc
    10321 ctctatacct aagcatataa atgtattcag cgttggcttt atgctttgac gcatcttgta
    10381 agcccatggt tcatttattg gaggatcaaa aaggctgtca attgccacta gggtatcctg
    10441 tgtaactatt attgcatcag caggaaagtg ttccccattt ataatgactc catcgctgat
    10501 tccgtctttt acagaaacct gtgttaccct gctcttgtaa tttattttac cacctaatga
    10561 ttcgaaatac tttgccatcc ggctagccat agccagtgat ccaccctctg gatagcctcc
    10621 atcacccgaa gcaagagttg cgattgtaaa tatcaaacca attgcggaat actcctctcc
    10681 aatgatacac ctaatcaaag ccctgagaat gggactttta aacctcattg caaactctcc
    10741 ggcagtttga tttgcataaa aagacattct tgagaatgca ggcagcattt taatgaaatc
    10801 tgataatggt actgaagtct tctttttcac ttttacgttc tttatatccc taataggcat
    10861 acttaccttg gagaaccttt ttatatccct gcataacttg tctatttcat ttctatcttc
    10921 aggcgcaagc tccagcaaat gttgccttaa cttttcaaca tcacggtaaa gaaatgctgt
    10981 cctaccctca aattccacag tcaggaaagg gtccttatta taaactggga catcatcgtt
    11041 cagcgcccca agttctctcc aaaccttatt aagcggcact tttggagagg atcccgtcaa
    11101 ccagtgcatt cctccctcaa aaaggtatcc ctttcttctc cagcttgtgg aggctccacc
    11161 tggtattgta tgactttcaa aaatggtaac atccattccg ctctgccttg cataaatgcc
    11221 tgctgatagt ccagcaatac ctgcaccaac aataattatc ttcttcatac tttacctcct
    11281 taatatatca actatccatt gaccctcagg gcatgggtat tcaggggtca ttgccatttg
    11341 ctcagcaatt ccctgtatag cgcaccaata tgctatggca agtgcatagg gttcaccttc
    11401 tttgattgag ccatttgctt ggcccttttt tatcaaatgt gaagttggag tataaatatc
    11461 aaatcccttt agcatatctt tcacactttg aggtgctgat tcattgtaat atgcttgact
    11521 cataagtaca aaatactttg caaaaaccgt atcagttttt agagaaccca aaatccaatc
    11581 tgcagtagtc ttaaaaaaaa caatgggctc catacccgaa tctgtcataa tactcatagg
    11641 gccagctaat ccattcttaa tgagttccac atacagcttc tcctttgatt cgaaatagtg
    11701 aaataataat cctgtgctca ttccaacctg atttgcaata tcacttatct ttgtggaagc
    11761 ataacctctg cggataaaaa gatctagccc tgcttcaagg atttcattcc ttcgtttata
    11821 tttttgttct tttcttgtaa ccaatttaca aagtctccta atcattaaat tttagttcat
    11881 taaatctata ttcaatatat taatccgtta tgctaactat ttcaagtctt tttctataaa
    11941 aaaacacctc ccattcgagg tgttaatcta ttttataaaa cttagtctaa ttatcttatg
    12001 aaataagtct tacaaaatga gtattcaatt atagcctgat tgagtttatg tactttttgc
    12061 ctttatttat gatacggctc ccccttgata atcctgaacc ccctgtatac ctgctccagc
    12121 agcatcaccc gcataagttg gtgaggaaag gtcatcgggg agaaggagag cttgaagtca
    12181 gcccgcttca tcacttcttc actgaggccc agagaactgc ctattataaa agctatattg
    12241 ctcctgccct ttatgcccag gtcgttgatg aagtcggcca gcccctctga ggatatctgc
    12301 cttccctgta tggcaagggc aattacatag gtatcctcct ttatgtgccg gagtatgcct
    12361 tgtccctctt tttgcatgac tgtcagctcc tgggcagggc ttaagttctc gggagccttt
    12421 tcgtccggta cttccataat atccagcttg cagtatttag aaagccgtgt tgagtattcc
    12481 tccacggctt cttttaaata tctttctttg agttttccga ctgtgattat cgatatattc
    12541 attacctgta ttcctccggc atttctgcca gctttatatc tgaattgtat tcggtattgc
    12601 tccttaagta ccttacactt acggtatccc cgggattcaa gctgtaaagc aggctcttga
    12661 actttacaag tgtgtttacc tcagtgccgt tgatatgagt tatcacatct cctgcctcca
    12721 gtcctgcgga tgctgcaggg gcacccttca taatattgta tatgtatatt cccttttgta
    12781 tctctatatt tattgcataa tagcccagca tctcgctgtc caggggagta atacccatat
    12841 atgtaggtat gaactcaccg gtatctataa gctttcggat tatcggcttt gtaatattta
    12901 tcggaatggc aaaacccagg ccttctgcag atgcctttac cgtatttatg cctattacct
    12961 gtcccctgcc gttaatcaag gggccgccgc tgttccccgg gttaatggcg gcatcggtct
    13021 gtataaggtc ctccattaca gagccctgtt ccaccataat gctcctgttg agcccggaca
    13081 ctataccctg ggtaactgtc ctttcaaaac gcatacccag aggatttcct attgctatgc
    13141 tgatttcccc tacaataagg ctgtcagagt ccccaagctc tgctgaaggt aaaccggttg
    13201 cctcaacctt taccactgcc agatccagga ggctgtcctg ccatagcacc gttccgtcca
    13261 gttcttttcc gtccttaaaa aacactgtca gttcctcagg atgctcaccc accacatggg
    13321 cgttagtaag tatgtatccc tttgggtcaa ttattacacc cgaccctact ccttcataag
    13381 acctcttgcc gaaaaacaag tccctctcca ctgtcaccgt acttatcctg actactgcgg
    13441 gggttacttt ctgtgccact gccgcagcta cattcatctc gttggatggc tgtatcacca
    13501 actgctgtcc tgaaggtgca atgcccccct gctgcacaga cagcattctg tcatacaaat
    13561 atgacggcac tattgcagca acaatgagtc ctcctattac ggcgcctacc agcgcagtgg
    13621 tcagaaataa aaatcccgag ggccttttat atcttatggg ctcattccta taagcagtcg
    13681 gagctgaagt attattctct tctgcagttt tattgccctg aatttccgga tatgaatttt
    13741 catcgaaatt atccataatt tcatcctcca cagccaaatt tattttatcc gggtaaatac
    13801 tctgatatta atatatccat ctgatgggca taaaatttca atcaatatta taaaacctgc
    13861 ttatcccgtt ccttggagca agatccagct taatatccgc atcaacattt atattgtttt
    13921 gtttcaatat cccctttact gtttcatagg caagctcagg gaagttgttt tcctggctca
    13981 ggtgtccaag aagcacttca cgcactcccg tctgcagaag ctcatttacg gcatttcccg
    14041 ccgcttcgtt ggaaaggtgc ccccgctcgc tcattatcct tctctttaaa aaaaatggat
    14101 atcttcccgt cataagcatc tcaatgtcat ggttggattc cagcaaaatc atgtcagagc
    14161 ctctgatatt ctcccttact gtatcgctga aatatccaag gtctgtagct atactaacct
    14221 tattattccc ttcatagaag ctgaagccta caggatcagc cgcatcatgg ggaatattga
    14281 atgacttgat tcctattcct gcaatctcca gttcttctcc tgtcttgaaa gatcttatgt
    14341 tttccggctt gaccgttcct atcaaagctc tcatgctgtt ccaggtatta atattggcat
    14401 atatcggtat tccaagcttc cttgatatga taccgacact cttgatatga tctgaatgct
    14461 catgagtaac tacgaccgct tttattgtac ttgaacaaac acctatgcta tgtaagtttt
    14521 tcagtgtttc cttgccgctt atgccgcagt ccaccagcaa tccgctgcgg tcattcccca
    14581 caaaaataca attcccgctg ctcccgcttg aaagggagca aagtcttatc cccatctctt
    14641 cctccattgc cgattacaac tgttagcatt gcacccttgt aatgtcagct cctgcatttc
    14701 ttaatttctc caccatgttt tcgtagcccc tgtcaatatg cttcagatta tctatttccg
    14761 tctccccttc agcagtcaat cctgctatta ccattgccgc tcccgctctc agatccgtgg
    14821 catgtaccgg agatcccgaa agcttgttta taccttcaat aattgcaacg cgtccttcta
    14881 ccttgatgtt ggcacccatc cgcttcagct catccacatg cttgaacctg ccttcccata
    14941 tgctctcggt gatgattcct gtgccctcgg atatggaaag cagcacgctc atgggctgct
    15001 gcagatcggt aggaaagccg ggatacggta ttgtcttaat attaactgcc tttggccttg
    15061 ccacagtcgt gacccttatg gagtctccgt tctcgtctac ttcaatgccc atttccctta
    15121 gctttgcaga taccgattca agatgtgtcg ggatgacatt ctttacaagc aaatccccct
    15181 gggtagcggc tgctgcaatc atgtaagtac ccgcttccat catatcgggt attatcatat
    15241 gctcacagct tccaagatgc tctactccct ttatctttat gacatcggtc cctgctccct
    15301 ttatattggc gcccattgca ttcaataggt tggcaatatc cactatgtat ggctcctttg
    15361 ccgcattttc tattgtggtt actccttttg ccttgcatgc agccagcatt atgtttatgg
    15421 tagcacctac gctcaccacg tccaggtata taggtgcgcc aaccaattcc gatgcttttg
    15481 ccttaataat accatgctca accttgattt tcgcccccag ggcttcaagg ccttttatgt
    15541 gttgatcaat aggccttatt cctatatcac agcctccggg aaaaggtact tcggctttct
    15601 tgaacttgcc cagaaacgct cccaaaaagt aataggaagc cctcattttc tttgctattt
    15661 caaaattcac cctgtagttt ttcatgccct tgctgatgac ttccagttcc ccggtatttc
    15721 catcattcac ctttgcgccg acttgctgca aaagcttctt aagatatctc acatcctcta
    15781 tatccggtaa atttttaata atacacccgg aatcggacag cagcgctgca ggtattatcc
    15841 ctaccgcaga gttttttgca ccgctgatac acacttcccc ctgtagtttt ttattccctt
    15901 taattataag tttttcttcc aaaagtatat gctcctttgt atgttaatga atgaaaatat
    15961 gccattttta ttatatcatt gatttatatt ttattaaata tattagaaaa aggcaatttt
    16021 attgcctttt ctcaattcct tccacttttt cattatatgc atttatatag ataatatccc
    16081 tgtcagtctc aatcttccat acagggaaag ccgttccttc cactacttca gaaacatcaa
    16141 cctgttctcc tgcaccggtt ccaaaataat atccctgacg gatttccacc acttccctgc
    16201 cgggcagctc acttttctta ttatgttccg gcacatacag cagcgctatc aacggagaaa
    16261 tcacttctgt cttcttccct gcctgcctta ttgtatcaaa ccatactatc cttgccctat
    16321 gcactccgga atcatttaca taaaactcca taatactgtt gtaaatctcc gctcctttga
    16381 atgactgcga aaaaagaaac ctcttatagc cttcaatatc ctccgatatt ccggtatcaa
    16441 gattatcagt tatattaagc ctgctcaaaa attccctaat attctttctg caagctttct
    16501 catctgtcgc cccggccggc tttatgccgt tgtctgaata gtaaaactcc cggttgtttc
    16561 taattgttat ttcaatattc tttcctttta accccactgt gttttctgag gctgattctt
    16621 ccggcacatc ccctgtggag aaaaagcttc cttttacatc ttctttgctg aaaagcttat
    16681 atttaactat aatagacggc atatcggctt ttttccccgg aattctgcct gttacagtta
    16741 catccttttc tgcaagatag tcagttattt gctttgcctt ctcactgtca acatatccaa
    16801 aaccacctgt gccggctgca aaaaccatat atccaagaaa gatattgatc gctgtaaaaa
    16861 caactataag aattgtcttt gcttttgccc aatccataaa aaccctctcc tgtatgtcct
    16921 gcactgacag ttccgtatta aggtatatcg gatcctattt atccagtatg atccctgtct
    16981 ctgcatttat tatatatttc tggttcagta ccgtaccctt gtcaatcctt tctaccgtaa
    17041 cttcggcaac ccaaacaggc acatatgtta tgctgttttg accgaatctt tcataatagg
    17101 ccaggtacat atcgttgact ttctttattc tttcaccgct taaggcctct acttttttgt
    17161 caagcagtat atcaagcaca tttatggaat tcatggcctg cttatactca aagttatggt
    17221 tcaatgtccg tctgatcctt ttatatcttc tgacttcatc ccctacgatc tcaacttcaa
    17281 tagagccgct gcttatatcg gaggctgaca gtacaggcaa tccctcatag gcatatctgt
    17341 atctgattat atacttgtca tcccccatac tgtttatcct aattatatca gatatatagc
    17401 aatcctcggg aaagccaaaa tgcctgctta caaattcagt gcttatatca atggcaccgc
    17461 ccaaaccgac cgtggttttg tcctccggct gcagattaaa attcacatat tccagcatgc
    17521 cgtcagtata cagtttaacc acctggccct ctctgttggt gtacacaata gtaccgtcca
    17581 tattcttaat tatgctgagg gaagttattt catcatcgaa caagcccgct atgctttcag
    17641 gtatctcatc cgacccttcc acctcctttt gtcccgacaa aaccggaaac ccatttgcgc
    17701 cgattctcgc cggcactgca gagtttttgc catacatctc cggttgaata ctgtcaagat
    17761 aaaggcttaa caccgtaggc tgcttttcca gattcgaaat cagataatcc aaattattct
    17821 gtattccttc tgtcctgaac tcataaagaa ttccctggac ggaatcacac agatatgccc
    17881 tgtccaggct ctgagcaacg attatctcat caatgctcct gatgcctgta tccggatagc
    17941 tgtttatatt cataagctta tatactgctt ccatctcaag aggcaaatta tagacgaagg
    18001 ttattcctct tgaatttctg attttgtaca attcctccat gggaatcctt ttcacaactt
    18061 ccttgctgtt caccaatatt gcagtacgca ttaccctctt ggcttccttg aatatcctgc
    18121 cgtatgcttc actgtattct tcattgtcat acagaagcgt acttctttct ccaaagctga
    18181 ctataagctt atgaggcctg agaagatccg atttgtcata ttccgcatta tagtccggtt
    18241 ttatcgattt gtttccggac ataataaaaa caccctcaat ggaagtataa aaccatattc
    18301 gggtagtcaa tacaaaactc aatattacaa gaaataaaag agatactgat ttaatccgtt
    18361 cgcggttcaa ttttatcacc tatatgggct tattcagtcg agttcacttt acttgataaa
    18421 tcctcaacga acttattcaa ggaaggacca ttgacttcct cttttgcatc cttctcaaga
    18481 ctgcgcacca attccaatgt tatttcttca gttacccctt ctgtattgct aatcaaaagc
    18541 acaattttgt ttttcccaag attaagggct accggttctg caaaaatccc tgaggatcct
    18601 actgtgtact cttttgaatc ctggaaaatc caatctcctt cattttccga agacgtcgac
    18661 tgtttcttat tgactataat gctctttgca ctcttaaacc aatacaattc tatagtaact
    18721 acagtacctt cacttgcact gccggtaatc atgtaatccc tgtcaaaggt ttttgattca
    18781 ttatctgaaa gcagcttaaa taccggggca gtttcttttg tatagtctgt ccctgattgg
    18841 gcatatactg ccgagcctgc tgcaaatacc gctgtaacaa tgattgctga aatcagcatc
    18901 ttcctgaaca tgagaattcc tcccaaattg atacatatta atcctcatta ttattatata
    18961 agaattttat tacaacatta ttacagacta attaaactta ttttacaata aactttcctc
    19021 gcaagggaaa ttaattataa cctcactgcc ttttccatat tcactttcta ttctgattga
    19081 gcctttatgg gcttccacaa tgttcttggc aattgaaagg cccagccctg taccccccat
    19141 ggatcttgac cgtgctttat caaccctgta aaacctttca aacagccttg gcagatcctc
    19201 ctttggtatt cctatcccgc tgtcggctat ccttatctct acgctatcct tcagtctttt
    19261 tgccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctat cccgctgtcg
    19381 gctatcctta tctctacgct atccttcagt ctttttgccg tcactttgat gctgccgttt
    19441 tccggagtat attttattgc gttgctgatt atatttatta ttacctgctc tattctatcc
    19501 ttatccccgt ttatttcaca cagctctcca atagcttcaa agcttaagga ctgatttttt
    19561 tgcttggcgg ccatctccat ctttacgaca caatctctta atatattcag tatactaaga
    19621 cttttcatgt tccattccat cttgtcataa tcaagcttgg aaagctgcag caaatccttt
    19681 accagcctgg tcatcctttc tgactcactg tttataacct ttagaaaatt caaggtatat
    19741 tccttgttat ccaatgctcc gtcaagtagg gtttcggtat agctcctgat tgtagtaagc
    19801 ggtgttcgca gctcgtgaga aacattggcc acaaattcct tgcgcatttt atccagcttt
    19861 tcctgttcgg ttacatcctg aagcaccagc atggcgcctt cagccttatt atgttcattt
    19921 attatctgca ctacactgat ttttattatg aggttgttta tgcttaaaat attgtaatta
    19981 ttatcactat cattaagaat atcgttgaag gttatcccaa gatccagctt ctctgcggca
    20041 tcgtcgaaat tcctgttata aaggtcttcc tcccggatat tgaaaatctt ataagcagca
    20101 ggatttgcat gtattactac cccttcggca tttacagcta ttatgccgtc ggtcatgttt
    20161 gtaagaattg cttcaacctt gttcttttcc ctggacattt catccaatgt gctcttcagc
    20221 ctcaccgtaa gatagttgaa catttcggtc agctggccta cctcatctct ggattttacg
    20281 gctattgtct gcccaaaatc cccctttgcc agtctctcag cctttgaagt tacttctttg
    20341 atgggacctg taaaagtcct tgacagcaga ttgcccagaa gcgcagttat gcatagtgcc
    20401 aaaatagtcg cactggtaag tattttattg atgctcatta tgttttcttc aataccgaat
    20461 aaatctgcac tgatatatat tgccccggct acatcattat tagaggaaat aataggcacg
    20521 gcaatactca tgagatattt tgagttatca acgggccatg tgatttcctg tgcttggtct
    20581 atagcttcct ccactatcca gagccccagg aggtcaagat catccacagc tccttcataa
    20641 ttggtctggc tggctgtaaa gcccccgttt tcatacacaa aaacagccct tacctgctta
    20701 ccctggccta caaaccaatt tgacacaatc tcctgaatat tagacgctcc ccctattttg
    20761 tccttcagat tgtcagccag ataatagccc cttgtgataa tagtatctgt catgctgtca
    20821 atctgttcct tttttatagc ttcggtgatg aatacgccta ctatggacat tgcaagccat
    20881 accaacagga aggatataat tacaagcttc cattggatgc ttttaaacat ctattaaatc
    20941 ctcctgaagt aatatcctac gcccctcttt gtctgcacat acacggcatt gctcgggtca
    21001 tcctcaatct tctctctcag tcttcttacc gtaacatcaa cagtccttac atccccgtag
    21061 tattcataac cccagacctc ttccagaagc gtttcccttg aaaagacctg ttcggcatga
    21121 agtgccagat atctcaaaag ctcaaattct cttagagtaa gctccagtac ggtatcatcc
    21181 ttgcggacct cgtatttgtt caaatcaatt atcagattgc ctgattttac aacctgcccg
    21241 ctgccttcca aaccggaatt aaggaaattg gcccttctta tgttggcctt gactctggcc
    21301 ataagctccc gcacgctgaa gggctttgtc atatagtcat ccgcacccag ctccaggccc
    21361 agaaccttgt ccacttcctc ttcctttgcc gtaagcatta ttatcggagt gctgacattc
    21421 tccctcagct tcttgcacac agtaaagcca tccattatag gaagcataat atcaagtata
    21481 ataagatccg gtttatgctc gtaggtcatg ttcacagcct gttgtccgtc ttcggccaca
    21541 aataccttgt acccttcctt ctccaggttg aattttatga tatctgaaat tggcctttca
    21601 tcatctacca caagaattct tctgttttcc atcaaaatac accaccccat taatatatag
    21661 aaaaatgtga cacaggtaac gcataacact gaaaacaccg aaatccacag aattcactgc
    21721 gggaaaaata aaatcagccg tgtcctccgt gttaaatccg tgtgttccgt gatacccgtt
    21781 ccataatacc cataaggaat accctgaata tctcgtgtcc gaagaatgcg acaaagtcgc
    21841 attcttctta cacatatttt actacttatg ctcctattac gcaacaagtc aggtttttcc
    21901 ggcggataca gtacaaaagg acgttagtac taacatcaat taaggaccat tgtcctatta
    21961 aaataaggcc tgtaccttat actaattttg gaaagagtaa tataaaatgt aaacatctca
    22021 acaatatgtt ttatatcccc ccggatataa agaaggcggt cttttcgacc gctctttttt
    22081 ttctacctct tccaggcacc tgaatcaata tattcattca gatcttctcc gtatattcta
    22141 ataaaagcct catccagggt gcttccatat ttaagttcgt ccagcatgag gtttatgcta
    22201 ttccttcccc tctgctcaat aagactccat acaatatcga aggactgcct gtaggactgt
    22261 gtttcatcca gttccataaa gtcatcccta agctcatggg cagtgtagtg cctttcatag
    22321 ctaaacccct ttgcccactc tgtcccatcc acatcatact cctcataaag ggcaagtcct
    22381 tccgtaagcc acagctctac gttccctccg cttctatagt caatcaccct gtgtacgtac
    22441 tcatgaagta tcggcccgtc cgcttcataa tccttggcat atatttgcat gctgtccaga
    22501 tatgctttgg gtgacagcac gtgaataata cccagattgt aaagccccat aactgcttgt
    22561 cctttgacag actgctgata atcccacatg ctttcctgat cattgtacac aaacactatg
    22621 gttttatcct ttgaataaaa accgtagtgt attccttcta ctttatagct cttttcaaaa
    22681 attcccgcta tgtctcttac tgtatcctca tctttatcgg tatacttaat gatgaaatgc
    22741 tccgtctcat atatattgaa ctcaggggtg aattcactgc tttttatatg agcaaatact
    22801 ttgagggctt catatccctg catttttaca gccataaagc ctttatcgcc ggtgaaaaaa
    22861 aatagtactg ctgctaatat tagtactatt gccggttttg atattctttt tttcatatac
    22921 ttaatccctt ctaacaagag caataactcc tgaaaatcag tgaatcaaat atattatatc
    22981 aaaattttat gttaacttca ttggtatttt ttgataccct gatactctaa tggcagccgc
    23041 tgcagcctcc gcagcttccg ctgctgcagc ctccaacccc ttttgatgat cccacactgc
    23101 agcattttcc ttcaaaaacc tgttttatat cctcgcttcc gcattttgga cactttatgt
    23161 tctctctgtc cgatagcttt acaatctctt caaatttttc accgcacttg atgcatttaa
    23221 aatctattaa agccatgagc aacactcctt tactataaat atttatatac cattcaccaa
    23281 tttttttcaa cagcatatta tgaaattagg cttttgctat tgataaatca ataataccgg
    23341 gggataacca tgatttcaat ttccttatgt ttaaaaagtt ttaaaaaggt tgatccggca
    23401 gtaaaaagat atatttccat caaaaaggtt aaaactatac ctgttattat aactttcaaa
    23461 aggcctcttg atccgaaaac cgaatcggca ctgaagcgga agggtctcag gataaaatac
    23521 catattccct ttctaaatgc agtcaccggt aaaataagta tcggtaattt tgattctgtc
    23581 agtagttttg tcgaaattgc aaaaatatat tttgacggta ctgcaaaact tatgggcggc
    23641 actgaaagcg gggataaggc ccttgaaagc cccgaagcag cctctgttca tttgagcgga
    23701 aaaggcataa ctgccgcctt tattgattcc ggcgtgtatc cccacccgga cctgacaaga
    23761 ccccggaaca gaatcacagc ctttaaggat tatattaatg aaatcaatga gccttatgat
    23821 gataacggcc atggcaccgc ctgtatagga gcggcctttg gagcctctat tgacggaaag
    23881 ttcaaggcag cagcttatga ctgcaatatt gtatgtgcaa aggccttcaa cagcttgggc
    23941 tatggatgct tctcggatat attggctgca atgcagtgga tatacagtat aaaggaaaaa
    24001 tacaacatca aggtccttgt gcttcccttt ggctgctgct gcacaagcag gcatttcgat
    24061 atcctgagcc tggcatcgga ggccctttgg aacaacggcc tatttatctg cacctgtgcc
    24121 ggaaacctgg gccctcatga ggccagtata acttctcccg gaatttgcgg ttccgccttc
    24181 acaacaggag catgtactgt ttccggatct tcttcaagag ttgcaccctt cagcggccgc
    24241 ggtcctgtgg atggcaagtt tgacaaacct gatgcagtta tgcccggata taagatagca
    24301 acccttaatg ccgataccga ttacataccg ggaagtaaat cgtcatacca gtcaaagctt
    24361 tcagggcagc aatacactca actaagcggg acctctgctg cggcaagcat gactgccgct
    24421 gccgcagtgc ttctgtatca gaaaaagcct aacctgccgc cttcagatat taaaagcctg
    24481 cttaagagat tctgcatctc actgaatgag cttaagaccg ctcagggagc cggtattatt
    24541 gaaataaaaa agattgggga attcaaattt taattttaat ccctcaatct ttttatatat
    24601 acttcatatt actttaaata gtttgccgga ttctgtatga cgccgttctt gcgtatctca
    24661 aaatgtacat gaggccccgt gctgtttccc gagctgccaa cctcgcctat cttctggccc
    24721 ttgtaaacct tctgtccgac ctttacactg tacttgctga gatgtccgta gtatgataca
    24781 aaattggcac cgtgatctat tattataagc ttgccgtatc cgccttgata accgacaaag
    24841 gtcacttctc ctccatccga agcttttaca tcagaaccat aaggtgctcc gatatctata
    24901 cctgtatgcc ttctgcccca tcgcattcca tatcttgatg ttatgcttcc cctggccgga
    24961 tatgagaacg ttcctgttcc cttcttgggc ggcggctctt tggtcccttt aacgattatc
    25021 tgtgttttgg gctctttgag cattttctca ctgagaatcg tccttccggt ctcaatgccg
    25081 ttaacctttg taacatcggc aacaacttct ttttcaccat atatgccttt gacccttata
    25141 ctagtctcat ccttatataa tgagctgctg aattccacct tctgatcaaa ttccgccttc
    25201 tcaataaatg tgatggtttc caccgtcttt acgctgataa gcggcttggg aacaatcagg
    25261 tttatcttct ggccgatctg aatcctttcg ggattaactc ccggattggc cttttccaaa
    25321 tccagaagag acatattgta ctttcgtgat atggaccaga agctttcgcc ggactttacc
    25381 tcatggatcc tgatttcgtt ggtccccttt ataataaaat ctattatctc gtctttatca
    25441 gctatttccg aagcatctat aaactcatcc ctaagctcta ccttttccgc aaaaccaatt
    25501 tccttgagct ttgacctgtc tgctcccgcc aggctcctgt cctgaatctc cttcaaaagc
    25561 tcctcggcct tttcctttgt cttcagaatt gcaataatat taccgtctgc atataagctg
    25621 cacccctgaa ccttgaattc aacattcttg cccaatactt caaccaatgc atcgtcctta
    25681 agtatttcct tgcttgatgc tcttgttttt tcatatgtaa cttcgctggt gaagcttatt
    25741 tcggaatcag ccttttcctg gtatttctgc ttcaaaatat ttaatgcgct ttcgaccgtc
    25801 tttgggtttt taactttaca gatttcaact ccattcacct tcacggtgta cgctttccct
    25861 gaagcataga caaaaatcga ggcaaatacc aggagtgcaa tcaatccgca ggctatggca
    25921 aaccttcttt tgttcacaat ttttctggaa tatgtatgtt tatttaatga attagctccg
    25981 ttatttgcta tctttttaat tatattttta atatgctttt taatattttc aattccgcca
    26041 gaaatcttgt tccagtaccg tgctgctgct tcctttaact tacttccctc catctattcg
    26101 cctccttcag cagtaattac ggttttgtca gctatatgta tctggcacaa gacatatagt
    26161 ctgcatagaa ctattctaca ataaaaacag agaatattca aatttttatg tttacgtcca
    26221 ttctttacca tctactgtgt aagcgcttgt aattattagt attcaaagct gcccttttta
    26281 ttcacttttt ttgtacaagc agtactataa acgacatata aatatgtcat atgtggtata
    26341 attatgatat acgcttaaat agttccaagg agtgcattat ttatgaactt gttcagaata
    26401 aaatcaaata acgaggatct tgggcaaacc attgttgaga accttttcat aagccattac
    26461 atgccctttg caccggcaga ttatgtcaag gtatacctgt tggggctcaa gtattctcaa
    26521 agttatgtaa acaatatgct ttccaccgat accgtagcaa agaccctcgg tatcacccgg
    26581 gatgaggtgc atgctgcttg gaggtactgg tcggagcaag atataatcaa gctgtatccc
    26641 tactccccgg atgatgctga aagcggcttt acggttgaat atctcagtat caaggaagtc
    26701 atgctgaata taaaggaaga aaagccgagt gcaggtaaat atagtcctga gcgcataata
    26761 aatgcccggg gcaatcagga tgtaagggct atgtttgatt ccttccgtaa actgttcgga
    26821 agagagctgt caccaaatga gctttttgta ttcctcgatt ggatggatga ctataacttt
    26881 cctcccgatg taattaaaat gctggtggag gaatgcgtat caagggatag gaaagagatg
    26941 ccatacctca agcaggtggc aaaaaattgg tttgacgccg gaatagactc tgtggacaaa
    27001 gcaaagcaat ataattcgcg ttataaggaa aaatggcaga agtacaacaa agtgctcagc
    27061 tttttaagaa ttggcaggca gccgacctcg gccgaagagg aaatgctgca gaagtggttc
    27121 tatacctttg aatacagcga ggatatagtg cttaaagcct gcgagctgac tgcaagcacc
    27181 ctgaagccat ccttcagtta tattgacagg atattgaccg aatggcacgg gaagggcctt
    27241 tccaccatgc ccgaggtgga ggcttacctg gttaagaacc cttcaaaggc cggcaggaat
    27301 tcaaagcccg aaaagaggac tttcaacaat tttgccaacc gtacatatga cgcaaaagca
    27361 cttgaagaag catggcttaa gaaaagcagg ggtgagttaa gtgaataaca atcaaataat
    27421 tcaaaaacaa tatgaactaa ttcgaaaaga aaacatggaa gagatagaag cacgtaagaa
    27481 aactctatat gataaattac ctaggcttaa agaaatagaa gatgaaatgg tgaagctaag
    27541 tattgacata actagagcta tacttagcaa atctaatact gctgaagttt taatagataa
    27601 actgaagaat aagcagcttg atttgaaagt agaaaaagca gaaatactat cagctaataa
    27661 gtatccaatt gattatcttg attccatata taaatgtaag ttgtgcaagg atacaggttt
    27721 tgtcaattac caaaaatgca aatgttataa acagagggaa atatacttga actatgagta
    27781 ttccaattta agttttatac tcgaaaaaga gaattttgag caattccgtt gggattatta
    27841 ttccgatgat cctgacgaaa atggcatatc ccctttgaaa aacatgcagg agatatataa
    27901 aaaatgcatc gactttgttg agcaatttga tgaacataat aaaaatctgt tttttatcgg
    27961 cagtcccgga ttaggaaaga catttctatg caactcgata gccaaggatc tgttggataa
    28021 aggtagaagt gttatttata tgactgttcc ggatttgatt gatacgatgc gaaaatataa
    28081 gtttgatttt gataaagaag aaaactatgc gcattattta aatgaaatat acaattgtga
    28141 ccttttaata atggatgatt tgggaactga gctttccact cagttttcca atcaggctat
    28201 atacaatata ctaaataaaa gaatagtcaa tatgaagaaa atgattatat ccacaaactt
    28261 aagcggcgta gattttcaaa gcacatactc cgagagaata gtttcaagaa ttattggtaa
    28321 ctttgaagcg tgcaagtttg tgggaaaaga tattcgtctt ataatgaaag gtatttcata
    28381 aagactcaaa agcaagtacc gtactgtgca acaaatgcca gtacggtctt gttatcccct
    28441 atcctgcttt aagttcatcc tgaatccttc ttataactgc ctttgcaaga gatcttatga
    28501 catctttatc actgctgatt tgtgcataaa actgtgagcg ttcttctgcc gcagacaaaa
    28561 tcccatttac aaatatctgt ccgattttat ccattgcttc tgcctcataa gcactgggtg
    28621 aactgtctct tacatttatt atcatttcat tgtcactgct taaagtattc acaatctcaa
    28681 ccaattcctg atgagcattt tcatatttgc cgtaacgagc atttattcta tatattatct
    28741 catcaattaa agaatagctt tcctctttaa cataagattc cgtcggcacc ctttccagcg
    28801 aataatctgc aacatttaca agcagatctt cttcctccga agccttttta actgagtatt
    28861 caacaagctc aaccaattcc cccggattga ttcttgtatc gtcggacttt tcgtaaagca
    28921 cattgtaaag agcatcaagg aacactgcaa atcgtttcat atcactgttc caaatgttat
    28981 atacaattga aacataataa aacaacttta caaattttga aatttcggca agaaataatt
    29041 tttttctgtc tttatccatc tcattatatt tttctttagc ttttgcaaat attgatgtca
    29101 attcacctga gtgtcttgca ttgtcattta ttagactata cgcgtattct gcctgttcaa
    29161 ttgtaaatac aaaataattc agaattctgt cccgctttgt aaaaagtata ttaggatctg
    29221 tttcattttc agagggcaag aacagctccc ccccgtagta ctgttcgaaa tatgctttta
    29281 tttcttccgt cttattcaca aaatcaagta taaaggtctt tttaccttta cagattctgt
    29341 tgattctgga cagagtctga actgctttta cagcactatt gagcttttta tcaacatata
    29401 ggatacatag ttttttctcg tcaaagcctg tctgcaattt gtctgcaacg attatgactc
    29461 tcacatcatc gttgtttttt attatttcct ttatgtcctt ttccttaaaa cccggattca
    29521 tattttcttc ggtatacttg attccgttaa gttctattgt tcccgtaaat gctgctattg
    29581 tctttatatt ataccccttt tttatcaagt acttatcaac cgccagcttg taacaaaccg
    29641 catgcttgcg ggagggggta ataatcatag cctttgcctt tccgtcaagc cagtttatac
    29701 gtttaatcaa aaaatcctcc ataattattt ccgttttgga atcaataacc tcggctgaac
    29761 tggtaatatg attcaaaatg gcggcggaag ctttagcggt ttcgtattcg gtaggatcat
    29821 ccttcttttt tgccagttca aatttctcat tataaattgt ataacatact atggggttca
    29881 atataaatcc ttcctgaata gcctgtttca tagagtattt gtggaagtat tttttcccga
    29941 tggcagtgcg tgtaccaaac agctctactg tttcggtctt tggagtagca gtaaaagcaa
    30001 aatagcttat gttttttgtt gacttgattt tgctcccttg cttcagaata cgattctgat
    30061 tatcctccag ttcctcgtat gtttcgtcta taagtatcga atccttcagg gattttccat
    30121 ccagggtctc cgccgtcttc gacaatgatt ttccgcctgt tgacgagtgc gcctcatcaa
    30181 ttataattgc ataattcctg ttggggatgt ttttcagctc atcaagaata tttccgaatt
    30241 tctgaagtgt ggtaataacg atatagcctc ccgacctaag catcttgaga agctctgccg
    30301 atgtagtgtc tatccacttt gctacaccct tctttccgtc aagtgtctgt atttcgatgc
    30361 ctaattggct gtcaataacg gtacggtcgc tgattacaat tacggtatgt atatcctgta
    30421 tattaatcag ccttttggaa agccatgcaa tagtctttgt tttgccgctt ccagcactat
    30481 gccagataag gtatcttccg cctataccgt tttgcttcac atccgcttca agcttcagca
    30541 ctgcatccag ctgatgatat ctgggaaata tcatttctcc gtcttcatca atgaacatga
    30601 actcacgaat aatctttaaa agcatatttt tccgaagaat ttcttcccat acatagttag
    30661 tacagtgctt gccttctaca attgggtttc ctgccccttc atcagaggat cccctgttga
    30721 agggaacaaa cacagtgttc tttctattaa ggcgggtagc agcaaaggct tcaaattcat
    30781 caacagcaaa atacaccaag gttctcttgt taaaaccgaa aataagctcg tcgggatttc
    30841 tcgtgtttct gaactgactt gttccttcaa agcccgcctt ctgtcctgta gtctgcttct
    30901 tcagttcaat cattattatg ggtataccat ttaaaaagat tgcaatatca actcgaaagc
    30961 tatcctgctt gtcttcatag gcaaattccc ggcaggcaga gaaaatattt ttttcgtaaa
    31021 gctccttgtt atatgtcata ctttcaatat ttgaaccaaa aaatgcaaga gaaaatccgg
    31081 tgctgctttg atagtcctcc agcttgccct tcaaagctgc aagcaggcct tcgttattga
    31141 ttttatcatt caatagctta ataaaccgtt ccatccagct atctttgtaa gtatccttaa
    31201 agctttcaag ctgctccttc tgggagttct ccagaaattc aaagagcttt gccctatcaa
    31261 ttgcattcat gtagtcatag tccgctttta tgctctcttt gtatccgata cttaaaagat
    31321 aattatatat ctcatctctg aatgggtttt ctcttaaaac ctctcctgcc aagcttttca
    31381 ccttcaatca catttcaatt ttccctgtca ccgcttcgta tataacactt tgtctgtatt
    31441 ccttaagctt tgtaatcata tcatcattgc tctttattat tcggtctatc tcgcagcatt
    31501 tttgatccag gtaatcggca atacgctgct gagtaggaag cgaaggtaca aaaaaactaa
    31561 tattgcttaa tgcacttgaa gttattgccg gatagcttac cccaactgat ctgctaacaa
    31621 cctcctgaat aaaatattcg ctttgggcct gataaaataa aaacctcggt aatagtccct
    31681 tctttgatgt taatactgcg aaaccggttg agcaaatata tttattgctc tcttcaataa
    31741 aagctatcgc ctttaaatat gttctcacag tcgatattat agtatctcct tttgacacaa
    31801 tcattcttgc tcttgaaggg cttgtatcaa aagtcatttc aacagtctct ccaatacctc
    31861 ccatttctgt gacactgctg atatctatat acttaaataa ataattcgga tctgtatcct
    31921 ctgataatct atttttatca acatcacaaa cgtattttat tttccacttt tcccaatcct
    31981 ccggtatctc cccaatccat tcaacaccac tatccttata ttttacattt ttgtcaagcc
    32041 cttttgttac cgcttcatat atgattgact gtttatattc ttttaaaagt tcattctgct
    32101 tctgctttgc agcaacaact ctatcaattt ccgcacactt ttgatcgaga tagttagcta
    32161 tttcgtcttg cttagctttc ttcggaaagg gcagcaaaaa attccctagg tcgctaccgg
    32221 ataattctaa aaatgtagta cctctgccta aagaattcag cagttcagca gatatcaata
    32281 aataataaaa ataaaaacgt tcattaacat tatcctttgc aaccaatgcc ttgcatcctt
    32341 gattagtaca tagctcaaca ccagctatac aaaccgatcc aataggtgca cgtgttgata
    32401 atataatact accagcagga actaatgatg tgccgcatga attcaatcct tcttcagtta
    32461 tttttctgtc tgaatctatg atgatattac taatacattt actcatatct gcaggcgtta
    32521 tccaagtaat atttccatcc caatattcaa tattactgct cgaaggtgta gccccattta
    32581 ctattttgaa taaatacttt gtctttactt cattaatact tatcttccca ttataaatta
    32641 tgcattgtga attatgcatt actctctcct ccccagcaga gtctcaatag taccttttat
    32701 atcatcctca atctcaatta tctcagataa tatatcccct gacttcctgg gtggaacata
    32761 tttatagaaa taccgggtaa aaggtatctc atatccaacc tttattttgt catgatctat
    32821 ccaggcatcc ggagcataag gcttaacctc cctattgaag tattcattga tatcttcctt
    32881 aaggggtata ttttccgtat cacgcaaagc tccatccgcc tgcacttttc ctttttttat
    32941 aatcggagca ccggtttcat cccttagggg cctttctatt gtaaccttat gatatccaaa
    33001 atcgatattg tcaaattttc ttgaatactc attctcctca aatttactgt aaagctccac
    33061 tatttttttt ctgttatgtg gggatataaa tttgcgctta ttaccgaggg atttcggctg
    33121 ctggggctcc caaaaatcta cggcatttat aagctgaacc tttttatccc gcttctctga
    33181 tttgtttctt gataatatcc agatatatga aaaaatacct gtgttataaa acatttgatc
    33241 cggcaaagcg acaattgctt caagaagatt gttctccagc acatagcggc ggatttcact
    33301 ttctccgctt tcggcatcgc cggaaaaaag cggagatcca ttcaatatta tagcaatcct
    33361 ggcgttcttc tctgcttttg ccagaaggtt ttgcaggaac agcagctgac cgtcgctgac
    33421 tcttggcgta cctgcaacaa aacggttatt tggactgttt agctcttttt ctacaatggc
    33481 tttttctgtt ttccactcca ctccaaaggg aggattggta atcagaatat caaacttctc
    33541 accttttaag tggtcttcag tcagggtatt tccctcatat atatttttaa catcctctcc
    33601 cagcatcagc atctctgcac agcatattgc atatgcttca ggattgttgt cctgtccgaa
    33661 aagacggact ctttcatcac tgccaagcaa ttcaccaagt ttcctcttgg ttactgtgag
    33721 catcccccct gtgccgcagc agccgtcata tacccttatt atcttccctt ccttttttaa
    33781 ttcatcaata ttctcggcta ccattaattc aacacaaagc tcaattactt ccctgggagt
    33841 atagtgatca cccgcttcgg cattggactt gtaagttctg attatttcct caaagatgta
    33901 tcccatagat aattggtcgg tagtatcagg atgaaagtca acttccgaaa aagccttcac
    33961 aacctcaaac agtattccct tttttatcat gaattcaact tcttctttga acttcaaacc
    34021 tttaattata ttcgctatgc ttggagaata ggaggtcagg aattccataa aatttgcatt
    34081 gatattatca ctatcgctga ttaagtcttc aaaggtgaca cacttcttgt tgtagaaggg
    34141 tattccgcat tccttctgta cagcctgttc aatcagtttt tcatctacat tcaacgattt
    34201 aaacttttcc agtattaatt ttattttgct gttctgtccc tcaagcacac agtcaaacct
    34261 tcttaagatt gacataggta taataacact gccgtactgc tcagatgtat aggggcctct
    34321 aagcttgttg gcaattttgg taacaagatt tacttttgca tttatatctg cttggtttat
    34381 catatttgtt ctccttcaaa caattttaac agttcaatat tcttaaacac tttatcccgc
    34441 tgcacctttt cttcctgaag caggcctttt tcaacaagct gcttcaaata tgccgatgcc
    34501 gtctgtctat gtattcccat ctcaacaata tcttcaatcc ttacatatac tttcttaaaa
    34561 atcagctcca tgacctgact caatactttt ttttgtccct ttaatgtctc caggaattct
    34621 tggtctgaat atttttctat aagcttttta atgctgttta ccttgttcag tgtgtcttcc
    34681 gccgttactt tgaaagaatt cagcatatat tctataatag atgtataatt atccttctgc
    34741 ctgcatgtct gtaataactc ataatactca ttcttgttct tgattatata tgagctggca
    34801 aataaaatag gatagtccag atatctcttt tggcataaca taagcacatt aagtatccgg
    34861 cctgttcttc cattaccgtc atagaaaggg tgtatacatt caaactggta atgtgccagt
    34921 gcaattttta ttaaaggatg aattgtgtat gtttcatcat catataaata ttcaagcata
    34981 tcaataagca gcttttggag cagctcctta ccatgaggag gggtatagag cacttcaatt
    35041 tcattgcccc gtttgtttgc aatcctggta aggctgttct caaactccgg catgttgacc
    35101 cttatgcctt ttgtcttttt attcactggt tcatttatgg cttcaatatc gccaatagac
    35161 aacacacctt tttccttcaa cctttcaaaa ccgataaaca atgcagtttt gtaattcgta
    35221 acttcttttg cttctgcagt ataattatca aatacaattc ctttatacag atcatcgttt
    35281 gtcgtgacaa tgttctcaat atggctgctt acctttgcct cctgcaaagt caacgtatca
    35341 atcattatag atggagatgg aagtgaattg atttcataat tcagtatttc aatagctacc
    35401 ctggtatctt ccgcaagctt taataatggc ttgttttctt caatagccaa ttgacttatc
    35461 ggtgtcaaat cattataagg ctttaattta tccattcttt cactctccta taagcttcaa
    35521 ggatagggtt tcccctatcc ttattcagct atacatacaa ataactaatt atccaccata
    35581 actccctgtc ttcttgtggc aatctataca aagaatttca caattggaca aattgtcgct
    35641 gccacctgca gccactgaaa tcttatgatg tgcttcccat gcaccccaat ttccatcttt
    35701 accacggtta tcgtaggaga gagttttatt acatctaata ctatggccat gtgatgaacg
    35761 tgtacactcg cacttaccac ctgaacgtat ccatgccgca tccactactg attgagaaaa
    35821 cgccattgtg gtaccttcct ttctccgatg gatattgaca aaatacagct tatcaaatat
    35881 tcttatagta tgcataaagc acgtgtgaac catcggattc acacaagacg gtgtctaatg
    35941 tcagttagat accgtctttt tgtctaacta tatttttaca tatatatttt tcgttgtcaa
    36001 tagattatat gtcgacatct tttaaaaata tcgacatata ataaagattt gtatataagt
    36061 gctgtgtttt ctcttaatct ttcttatgga ttaactacat ttaccggtgt acctgctagg
    36121 aatttctcca gattgtttac tgcaatatcc atcagacgtt tccttgactc ttttggtgcc
    36181 catgaaatat gaggagttat aatgcagttc tttgccttta acaatggatt atcaggcttt
    36241 atcggctcag tcatgacaac atccacagcc gctccggcta ctttgccgct gtttaaagct
    36301 tctgcaaggt cttcttcaac aatcaacggg cctcttgagt tatttataat cataactcca
    36361 tccttcatct ttgcaatatt ttccctgttt attattccct tcgtactttc aaacaacgga
    36421 cagtgcaaag caatcacatc agatttggca aacagctcat caagttcgac atatttcatt
    36481 gtatcactct ctaatgcggg attcttatac gaatcagctg caataaccct catgcccaat
    36541 gcctgggcaa tcctgcccgt tgtctggccg attctcccat atcctattat acccattgtt
    36601 ttcccggcca gttcgattaa aggaaagttc cagtagcacc aatcagggtt atttgcccac
    36661 tctcccctta ataccgagtc gctgtgtgca ccgacatgat ggcacatttc aagaagcaac
    36721 gcaattgcaa actgacctac tgaatccgta ccataggttg gaatatttgt aacaactact
    36781 cccttttcct tggcggcaac cacatcaaca atattatatc ctgttgccaa tactccgata
    36841 aatcttaagt tgggcatttt cttcattgcc cttcttgtaa caggagtctt gttagtaaaa
    36901 acaatttctg catcgccaac gctttccgct accatatctt catctttttc atcatatgat
    36961 acgcggtcat ataccgttat ttccccaagt ttttcaagtc cgtcccagct taaatccccg
    37021 gggtttaatg tatatccgtc aagtactacg attttcatgt tgactctcct ccttgtatta
    37081 ttaatagcat attatattat tttatggatt acagcatttt attatcttta ttattaaaca
    37141 tagcagtata aatatcaagt gcaaatcaaa ataaccgaag gctgcataat gcaaagttgt
    37201 ttcttatttc ttgaatttgc tgtatattac atatgaatac actatgggta taaaagtcat
    37261 aaccgcaatt actgctataa atgcccatcc catactcagc ctaagcagtg acatgatcac
    37321 tcctgccaaa ccgccggctg tccaaaggat agccccaagc ctgtgggttt ttctccatac
    37381 ctcttcatta gccaaggtcc agggggtttt tataccgaca aagtaattgt gcttcagcct
    37441 tcccatcaca ttgcccatga gcatgagcag cagtgaaact gccaaagtaa ccagccttcc
    37501 cgtatcaaca ttgtagccca gggctgatgc tatgatcagg ccgtgtatac ccaaaaagaa
    37561 gatgtggaaa aggtatctga aatatatata ggtgctttca aataatttat agttttcctt
    37621 tttgggatca atagcaggta taaatataaa gagcccgtac attattatat tcattacggg
    37681 cataaggaat gctcccgtca gctttcccga gtacccgtct atttctcctt taaaattcca
    37741 atgtgtaggg attttatccg gtaattgggg ataaaagtat atacttgctg caaacatcaa
    37801 gacaattatg agtatgatcg gcagttcttt tttaatgttt aaatcaatct ttttcatttt
    37861 caaaccctcc gcttctatct gtcatgttaa aaaaccaatt gaccacttcc tgaaacaccg
    37921 tagtattcaa tgaatagtaa atattctggc ctctcctttc gtctgtaacc agatcagcct
    37981 gcttcagaat gctaagatga tgagatatgc tgggttttga catttgaaaa ttgtcagcta
    38041 tttctccggc agtcatatct tttcccttca gcatctggat tatttttcgc ctgcttggat
    38101 cagacagggc tttaaacggc atattgagta tggacaattt catcgcctct tttattaata
    38161 taattagata tttagataaa tgtctaatta tattatatac aaaaacaatc aataagaaaa
    38221 ggggtatcgc taactattta ttatcttaaa tagttttacg aaccccttaa aatttcttat
    38281 ggagctggtg aacggaattg aaccatcaac ctgctgatta caagtcagct gctctgccta
    38341 ttgagctaca ccagcatatg gcgacccaga acgggctcga accgtcgacc tccagcgtga
    38401 caggctggca ttctaaccaa ctgaactact gggccaagta tttgcctccg caaatgctac
    38461 ggctttaatc aatgaatgtc ggtcttttta cttttacgct ctgcgggtca taaattgctc
    38521 cgcatgtgct acgcattaaa tcaaggaatg ttagtctttt tccgcgaagc ggaaaaagct
    38581 ggtggggaca atagggctcg aacctatgac cctctgcttg taaggcagat gctctcccag
    38641 ctgagctatg cccccatttt atggtgaccc gtgggggaat cgaacccccg ttaccgccgt
    38701 gaaagggcga tgtcttgacc gcttgaccaa cgggccatta atggtgactc accggagaat
    38761 cgaactccgg acaccatgat taaaagtcat gtgctctacc gactgagcta gtgagtcacg
    38821 atgtgagcaa tttttgcggc tcacatttat acattctaca atgtcagaat caatttgtca
    38881 atatacattt tctgcatctg ttgtttcaat tatatcacat gcatacctaa aacttatcaa
    38941 tcattattgt ttgatttctc tttgggccta cagagataat cgatatcttt gttccgcaga
    39001 tttcttcgat tctttccaga tagcgcagaa cacttgtggg cagcttgttg tattgggtaa
    39061 tatcaccgat atctccccag ccttcaaact cttcatatat cggttcacaa ttttccagat
    39121 cctccaagct tgcaggaaaa tccctgatta tttttccatc cagcctatag cctacgcaca
    39181 ttttaatagt cggaatattt gacagtgtat caagcttggt tatggcaata cctgtaagtc
    39241 cgctgattct cgcagcgtat ctgactataa cggcgtcaaa ccagccgcat ctccttggcc
    39301 ttcctgttac agtgccgaat tccttgcccg tagtcctgat atattctcct gtctcatcaa
    39361 aaagctctgt ggggaaagga ccctttccta ctctggttgt atatgctttt acaactccta
    39421 tgaccttgcc tatgagggca ggcccgactc ctgcgcctat acatactccc cctgcaatag
    39481 gatgggagga agtaacataa ggatatgttc cgaagtccag atccagcagc gttccctgtg
    39541 ctccttcgaa gagcactttc tttcctgctt taattgcatc gtaaatcaat accgatgtat
    39601 cttcgacaaa aggccttacc tgttccgcat agtccaggta tttggacagc atttcttcat
    39661 atgaatagcc ttcaaaatcg tatattttct ttagatatgc attcttcagc tccaaattgg
    39721 ttttgagctt ctttgcgaga acctcttttt tcaataaatc gcagactctt atcccgattc
    39781 gttctgtttt gtccgcataa caggggccta ttcctttttt ggttgtcccc agatcatcgc
    39841 tccctctttt aagctcggaa agctcatcaa tcttcttatg atacgggaat aatacatgcg
    39901 ctctgtcgct tatcctgaga tttcccgtac ttatgcctct gctttccaga tttctgatct
    39961 cattaagaaa tgcttcagga tcaataacaa ccccattgcc tatgatgcac attttatcag
    40021 cgtacaatat ccccgaagga ataagctgca gtttatatac ttcattatca acctctacag
    40081 tatgtccggc attattgcct ccctgatatc ttacaactac atccgctttt tcggcaagat
    40141 aatccgtgag ctttcccttt ccttcgtctc cccattgtgc tcctactatt accaatgatg
    40201 acatatataa cacctcttta attaatttct ctgagcttag ctgtgcttgc agtatttttc
    40261 ccccagcaaa cgggcaacat atactccgct tgctgaagcc tgggataacg aatgtgttac
    40321 tcctgagctg tcacccaaaa tatacagccc tttgattatt gtctccaggt tattgtcaac
    40381 ctctacctgg gaattataaa atttgacttc cacaccatac agcagcgtat cttcatttgc
    40441 tgttcccgga gcaattttgt ccaatgcata aatcatttct atgatatcat ccagctgtct
    40501 tttaggaatg acaaggctca aatctcccgg tgtagcattc agtgtcgggc gcagaaagct
    40561 tttttccata cgcctttcac tggatctgcg tccttttacc aagtcaccga atctctggac
    40621 caatactcct ccgcccagca tattgctgag ctttgctatt gattcaccat actcattgct
    40681 gttcttaaag ggctctgtaa atctgttgga aaccagcagc gcaaaattcg tattctcggt
    40741 atggtgcttg gggtctgaat aactgtgtcc gtttactgtt acaattccgt tcgtattttc
    40801 agtgaccact tttccatggg gattcatgca gaaggttctt accaaatcat tgtatttctc
    40861 tgtcctatat acaatcttgc tttcataaac ctggctggta atgtgctcaa atattgaagc
    40921 cggaatttca acccgcacac caatgtccac tctgttgctt tccgttttta tccccagctt
    40981 ttcacaaatc tgcgaaatcc attttgaacc tgaacgtccg gcagccagaa tcaaatgact
    41041 gcaagaatat tctccttttt ctgctgatac gataaaggta tctccttgtt tttcaatatc
    41101 cgatacttca ttattaaata ttatttctac cttatccatt aggtactcat acagattttt
    41161 cattattacg aggtttttgt ccgttcccat atgacgcacc ttggcatcca gcaaatggag
    41221 atcgtgtcgc aatgcctgcg ttctaaggct gctgttctcc gtagaataaa gcattgcgcc
    41281 ttcgccgccc attttgcaat ttacctcatc cacgtagtac ataaggtcaa gagcttcttt
    41341 cctgcctatg tatttgtaaa gctctccccc aaaattattg gttatgttgt attttccgtc
    41401 agacattcct cctgctccgc cgaatccgtt cataatactg caggtgggac agttaatgca
    41461 tgattttatt tttttattat ctataggaca tgctcttttt tccagctcat tccccttttc
    41521 cagtatagca accttaatac ctgcatcata ttgcataagc tcataagctg caaatattcc
    41581 gcttggccct gcgccgacta ttataacatc gtatttcatt agggcaccga cctccatctt
    41641 cacagcaata tcgtactgac agaatattgc tgtgcaaatt aaataagtat ttttattcaa
    41701 atttcatcct aacagaatat agtttccaag tcaatattat gaagcttgaa gttactgtca
    41761 agtaaacgtt ggcaacttac cttcatacaa atttttacta aaaatgtttt tgtcaatttg
    41821 gtcgcattta ttccacgatc ctattgacat ggggacccct gtaccctctg gacacctatt
    41881 tttaggcgga attagcctat attctttgcg cctttccgct gccaagattt taccagaggg
    41941 taccacccca tgtaaatagg ctttcgcggc ataaacgccc tgtttagata tattacctgt
    42001 aaactaaatc ggtgaaattt ctcatgtcaa acatgctgcg tattgctttc cttccattgg
    42061 taactggata attcgtaaat ggtctttggc ccttgtgtat atttccatct ttaccttttc
    42121 catctttttt cggtgctttt gacgaactta gtatctgaat tatctcaata gttttctctt
    42181 ccggtaatac aatctttgaa tgcctttcaa caacctcatt taatcgttta ctcgccttga
    42241 cagctaataa cttacctaaa ttattagctc cttcaatgct ccagcttgct ttacgatgtt
    42301 tcatcctctg ggctattatg tcacatatgt gatgttccat agtcccgaga ttgcgatact
    42361 ctatccctaa aggaggcttt ggaatatcta accctctttc ctggtaaggt attaagccgt
    42421 ctcggttctc tttaaagtat gagtataaat ccttaagctt cttttgctct ttctcatctt
    42481 ctgtatcatt tgctaatcct gcaatatacg ctaaagcctc attaatttcg ttgctattta
    42541 atagtttaag tatagtcttt ctttgctctc catcccttac acttttgagt attgccttat
    42601 gtttatgaaa aggatccagc tgataatgta ctgtatcatc tataccatgc tttatccagg
    42661 cagctccgtc accattaagt atcctcattc gtatctcatc tacattgtat tctcttgata
    42721 tcatcgcttc tttaattttg taaaactcag atatatcctc aaagcccgca caggctactt
    42781 tgtttaccag ttcaaatcta ttcttgctaa cttctttcca gccttcatat gctacggcta
    42841 ctttcatttc aagcttctta cctgacttcg gacggtcttt accttggatg tttatataaa
    42901 ccccgtcagc ttcttcaaaa agaaccggaa cttcttttcc cccgcacaac tgattcttct
    42961 ctgctgcttt agccagttct gtttcttctt cccttacctt ttgccctaaa gcctgaacca
    43021 cattccatac gccaccatgg ctgatgcctt gtcctgttag ctcagttata ttatctgcag
    43081 cattgcggta agatgttatg ctggcatttt ctactatctt ttccgccagg tttgttgata
    43141 tctgccctat tgtatcgaat cctaaaagtt catctaacaa atatatgaat gactttctgt
    43201 cttcatcatc tttaccttca taaatcacac gggaaaattc cacttctccc ataagagttt
    43261 ttattgatgt gttccttccc cctctatgtc tatattttga tttatctctt tcttctgcga
    43321 gcctcttatc cattgcctct aaaacacttt taagaatttc gcgagctatc tggcaaccat
    43381 aagcataaat ttccttttct aacctattga atgataaatc cttttcattt acaattatat
    43441 caatcataca aatcactcct gtctatgttt tcttgacaat tacattttac agtttgattt
    43501 gtatgatggg aaggggtgtt ttgcctcttc ctttattttt gccaattatt ttgccaatga
    43561 aaattatact ctaagaagct tgaagattgt tattattagt cctgcttgta ttattatatg
    43621 gattttaatt aataattatg gaactgtgta tttcatttta atagcagcgt tggcgcaatg
    43681 cggaaactag ttttctgaaa attctgattc acattatcca cattatccac agagatatgc
    43741 acatatgttc ggctggctgt gcaaattctc ttgtctcaat gtatatactg ccgtgcataa
    43801 ttatcattca gaatttatgt taatcgagca acttaattat tcacaatatc cccaggttat
    43861 ccacaggcaa aaccatgttc aagttttaga aaacaaggag ccttctacaa aatactacga
    43921 aagctccaat aaaacaacaa aattttataa ttatgtaaat taaattgctg ttatatgctt
    43981 ttccttgttc acaaattttg tgaactgccc cagccaaatc agttcaatag taccggtggg
    44041 tccattcctc tgcttggaaa tattaatttc tccgatattc tttttttctg tatcaggatg
    44101 gtagtattca tctctatata ggaacattac aacatcagca tcctgttcta tagctcccga
    44161 ctctctcaga tctgagagaa ccggtctgtg atctgctctc atttcaggtg cgcgggaaag
    44221 ctgggacaac gctaccaccg gaacattgat ttctctggct aaagctttta gtgatcttga
    44281 tatttcagat atctcctgct gcctgctttc ggcatagcct ttcccctgca taagctgtaa
    44341 atagtctatt acaataagtc caaggtcatg ttccagcttg agcctcctgc actttgccct
    44401 catttcggtt atagaaatag aaggggtatc atcaatataa ataggtgctt ccgaaagagg
    44461 gcccattgcc cttgcaagct ttacccattc atcctcattg atatcgccta tccttaagcg
    44521 gtgagcatca ataagagctt ccgaacacaa cagcctctga gtaagctgct ccttggacat
    44581 ttccaggctg aatattgcag tggtagtttt ttcccttacc gctgcgtgct ggactatgtt
    44641 catcataaac gaggatttcc ccatagaagg ccttgcggca ataagtataa ggtcagactt
    44701 ctgtaggccc gaaagcttat tgtccagatc atgaaagccg gtaggaactc ctgtgatttt
    44761 tcccttgttc agatacaatt gctctattct gtcaaagttt gcgttaagta tgttttttag
    44821 atgaacaaag ccttgggtag accttttcag ggatatatta aatatgcctt tttcagcatc
    44881 atcaataatt tcccctatat cctcttcagc ctgatagctc ttctggatta tctcgttgga
    44941 tgatttgata agctttctaa gcaaagactt gtcttctaca attttggcat aatacttgac
    45001 attgtgagtc gtcggaacag catccataag gttggtcaga aaagttattc ctcccactgc
    45061 ctccaatgta tttctggtct tcagcttctc agtgagtgta ataaggtcta ccggctcccc
    45121 cctgttgtat atatccacta ttgcctcaaa tatctcctta tgtgcttccc tgtaaaagtc
    45181 atctcctgaa atatactctg tggctgtgga tatagcctcc ttatcaagga gcatggctcc
    45241 cagaaccgat tgttcggctt caatattgtt gggcggtatt ctctgtatca tttcactcat
    45301 ttcatccact cctagaatct attgttcatc aaacataaaa ccgatcactt cttgaatatt
    45361 gcctgcggga attatattta ttccatccag gccgttggct tcttccttgt tttcttcagg
    45421 aattatgaca ttcttgaagc ctgctctctt ggctcctgaa attttttcaa cgatgccgcc
    45481 cactgccttg atacggcctc ttatggatat ttccccggta atgcaggtat cctgtctggc
    45541 aggccgctcc aatattgccg aatacaaaag gcaggttatt gcagctcctg cggaaggccc
    45601 gtcaaccttc ccgccgccta cgcagttaac atggatatca tatttgttaa tatttttatc
    45661 cgttattttc cttataaccg aaagagcatt gaataaagaa tcctttgtca tactgccggc
    45721 tgtctcattg aaccttatgg tgcccgcttc atcttctctg gcaaagcata ctgcttccaa
    45781 ttcgattaca ttgccgataa agccgttcac tccaaggccg ttaatccgtc cgatttctct
    45841 gatgccctcc gcttttttct tgttcaacgg cacgtatctg cctttgctta atacttcctt
    45901 tatacaatca acggatattt ctatatcctt gtcttccctt tcatttatca tgctgctgta
    45961 gcagtcagca agaatattga ctgccttcct ggcttcgtat gtataactgc tgatcaactc
    46021 tgcccccttt gcacttattc gggcaccaag cttatcagcc gcattcaata ctattctggc
    46081 aatatcggaa ctgcttaaag gattgaagaa cacttccgca cagcgtgacc gaagagccgg
    46141 gtttatttct tccggctgcc tggtggttgc tccgataaga atgaaatcgg caggagcacc
    46201 ttcttcaaac agaagcttta tatatcgagg tatgttttcg ttattgacat cataatatga
    46261 ggaatcaaac cttaccttct tgtcctccag caccttaagc agcttgttct ggagcatcac
    46321 atccaactca cctatttcat ctataaaaag cacacctccg tgagcttcgg taaccaggcc
    46381 gggtttaggc tcaggaatac ccatttctgc aagctccttg ttggctccct gaaaaatagg
    46441 gtcatgaacc gaacctaaca ggggatttat tgattccttc ggatcccatc ttagggtggt
    46501 tgcatcaact tctacaaact tggcagtttt gctgaaggga gtatgtttcc ggcttttcgc
    46561 gatttcaagg gcaagacggg ctgtggtagt cttgcctacg cccggaggcc cgtacaatat
    46621 aacatgctga ggaaagggtg aggctatctt ggatatcaaa gctgaaacag cattctcctg
    46681 tccaattatt tcatcctgcc tttgagggcg cattacatcc attattgatt ttgtaagcct
    46741 taccttcttc atcagctcca agtccgcata ttttttcagg gtagaagcat tctcaagccc
    46801 tgcatgcttt tttataacct gcagcttcat ttcattcaag tattctattt cccttgtttc
    46861 catgcgcttt atgacttccc gttttatgcc ttcttctatg tatcttttgg ctgcggcatc
    46921 agaaagcttg tccttaatat cttcaattaa aaattccagc ttgttctctt ctattctctt
    46981 gttttctatt ttgccggaaa ctatttcatg cagtatataa atcttttctt tattatctgt
    47041 aacattatcc agttttctgg tcttatcact agaatacaat ttttttgtaa aatcaacaag
    47101 tacgtcaatt gatacatctt tgctgcgtat gctgctatgg ttatggctca ccttttagac
    47161 ccctctctgt tcttcaactt ttacatttat taatgtggat atcccgggat ataccttaat
    47221 ttcaacctta tatgtcccca atgccttaat ggcatcatga agcactatct ttttcttgtc
    47281 tatctctgtc ttgtgctgtg attttaaagc atcagccaca tccttgcttg ttatggaacc
    47341 gaacaatttg ccgttctctc ccgcttttac cttgaagttt acctccaatt tttcaagctt
    47401 gtcggcaagc tcctttgctg cttgtttctc tttatccttc tttattattt cagaattttt
    47461 cttttcctcc agttctttaa gcttccctga agtagcctct gctgcaagtt ttctcgggaa
    47521 cagatagttc ctggcatagc cgtcgctgac attgaccaat tgttcctttt ttcccagatt
    47581 cttaacatcc tgtaaaagta caaccttcat ttatctttca ccttccttaa gatattcctc
    47641 tatggctttt ttcaataagc tcatggcctt ttccatagta agccccggaa gctgtgcgcc
    47701 ggccacagtg agatgtccgc ctccgccaag cttttccagc accacctgca cattaatatc
    47761 tcccagggat cttccgctta tgaaaatatc cccctcaatg caggaaagta cgaaggaagc
    47821 atttacacct ttgatattca acagctcatc agcggcttga gctgcaacca gagcaggatt
    47881 ctttgcattc accggtatag tggaaattgc tatattgctc ttgaacattt gcgctccctt
    47941 tactacctca gcccgagtca aaaaagttgc catgtcatcc tggaaaagct gctttgtcag
    48001 ggtagtatcc gctcccatcc ttctaagata ggatgcagcc tcaaaggttc tgacccctgt
    48061 ctggaaagag aaattcttgg tatctataaa tatccctgcg agcagggctt ccgcttctat
    48121 ctgctttatc ttcggctttt caaacatgta ttgcagcaac tcggttacca gctcactggt
    48181 agaagatgca tagggctcta tgtatgtgat aaccgcattc tcaatgaatt cgctgcctct
    48241 tctgtgatga tctattattg caattttctc agctttttct acaagctgct tttgcactgt
    48301 aaatgccggc ctgtgcacat ctacaaccac cagcaaagta tttgccccgc aaatttgaag
    48361 ggcatcctgc gggctgacaa atacatccgc atattcctca tcctcctgca gctccgacca
    48421 taggtttgct atacctgcat ttacaccgcc aagtactatt ttcacttcct tgttcaggcc
    48481 tttgcagcac ctgtatatgc ccaaggcgga acccatacag tcaaggtcag gcatttcatg
    48541 gcccataata agcacatcct cggactgctc aataagctgt cttaatgcat aggctattac
    48601 ccttgccttt actcttgtcc tcttctcaac ggcctttgtc ttgcctccat aaaaggaaag
    48661 cttttcatta tccttgacaa ccgcctggtc tccccctctg cccagagcaa tatccatggc
    48721 gcttgtacag aacttctgca gctccgcatg gttcttcccg ttaactccaa cgcctatgct
    48781 taatgttatg ggaattttgt tgcctgaatt aatttccctg atgctgtcaa gtatgtcaaa
    48841 acgtttttcc tccattgaca tcagctgctt atgactgaaa taaatcatgt attccccgtt
    48901 ggaatatttc tggatggatg cgttgttaag tgccgcccat gcattgaccc ttctgtcaac
    48961 ttccgcaacc agggcaggcc ttaacgcttc ctctgtctct ttaattacct cttctatgtt
    49021 atccacctga agtatagccg ctatgggctt ttcgtcgtta tatctgttcc tgaggctgat
    49081 aaagtctgtc ttatccacaa aatacattat tattatattc ttttcctcgc cgctttcatt
    49141 gatattaatc aaattgcagt atatatcata gtatctgtct ttgatattga tattcttgaa
    49201 cctttccatt ttattcttca gtatcctgct gatatccaca ccgcttaaat aggacgacag
    49261 cttcttgtca agtatgtact ctccttggaa aatactcgag aaaagcatat tataccaatt
    49321 gataacgccc tgatcatcaa taataaccaa tgggaacggc aggttaagaa tggcattttt
    49381 ggttgcagta tctacatttt cagataagtt ttctatatat aatctgagtt cttccttttt
    49441 tctggttgcg gcatgaagat tgtggtaaac cagatatccc agaagcagtg cgccggccaa
    49501 acccaaaaaa acattgtaat agcctatgac tactacggca atggcgagta tccaaagaga
    49561 tatgctcaca tccggccccc atatgtccga aaacttgcga attttcatct cacacctcca
    49621 atgcgatttt tggatttcag ccgcctaaaa tcaaacgcca tatctaatat gccgataaag
    49681 gctataaact gaataaaggt aagaatgaca aaaacagtta ttattctccg taatgctttc
    49741 gggataccgt atctatcccc ataaaccctt gcgaaataaa caactgccga tattccgaca
    49801 atagagaaaa tcagtgatag tatagtagag atgtttaaaa cgactgcatt gaatccggga
    49861 accttaaagt atgatgctac ccctgcaaga acagccgttc caagcaatat gtatgaatat
    49921 ggttcggtaa gcctccattt tgaaaattca tctatgtcgg gcaaaataag gccgattctt
    49981 ttcagtatta gtcttgtaag cttataatca ataaatgaga aaaatacgcc tgatatcagc
    50041 aatgaagaag gaatcacata cttcatcagc tctattgcct gactaaagta atctttgaat
    50101 tcctgcaatt tttcctctgc agcaccgaac tgcctatata tattcaagga tgtttccaac
    50161 tgctccctga aggcagaatc aagatttgat aaaaattcca atccgtcaat acctgcaacc
    50221 tttaatgaca gcagaaattc tcccgccatg cttacggaca gtactacacc gcacactaat
    50281 attactgcat acggattcac ccttttgttt atcatatgcc ccattaatac tcccgggaca
    50341 ccaaaaccaa tcagaagccc aacccctacc agaggctgcg tcaccagaga aaccaccaac
    50401 ccggcagata aggcagacag tatactcgct ttaatgccgt ttctatagcc cactattatt
    50461 accggtacag gccaaaccat agctattatt ccgagatact gcgtataata atacagtatt
    50521 cccaaaagtg cagtgacacc tgcaaatata gcaccttcta ccagtgccct tgtttgcatt
    50581 ctctccatgc tgagcctcta gcctttcttc gttttcaaat aatcgccaag gtcagtcagg
    50641 tccccgtacc atttttccgt ctcgtgctcc tccagcattc ctaaccgtat cttttccagt
    50701 atcttggtat caatcattga atatggtact ccaagccttt taccaagcag atatgtaacc
    50761 agtacaaggt ttgccagtat atcctgaatg accccctgcc ccacctttgt acctttcagc
    50821 agattctgat acagtgatgc aacggtggtt aagagttcgc ttttcaaaaa ctctatcatt
    50881 tttatatttt ttgtaatgtc agtgtcctgg tttaacaagc tgatcccccc attacattat
    50941 ttgaccttgt ccactattaa tattatattc catatataat aaaaaattag tcaaatcatg
    51001 ttcgggaagc ttattttata ttaccacaat tcgtcaaagt aaacaacaaa tcaaaatgtc
    51061 ttgtaatctt tggaaccact ggatttgcaa gccatataac cttccaatgc caaacagcct
    51121 aacctgtttt ttaattcgac atactaaata taataccttc ccgaaaaatc cttttaaata
    51181 ttggaatcag cggtattgaa tagcatttca taatccaaaa aataaaaccc gggggcaaaa
    51241 aacctccgag tccaatattt attcttctaa tcggtagtaa agggcaggaa agctatgttc
    51301 cttgctctct ttattgctat tgtgagctgt ctctgatgct ttgcgcagtt tccggaaatt
    51361 cttctcggaa ttatcttgcc tctttcagag atgaatcttc tcagcttgtt tacatctttg
    51421 taatctatat gttcaacttt atcaatacaa aagttacaaa cttttttctt tggctttctc
    51481 atttttccgc cgccggttct ttcaccgcca ctacgtctgt tcaatcattt tccctccaat
    51541 ctgtatgttt tatgtaacat atctgctaaa acggaagctc atcctcttct tcagccaggt
    51601 ggaaatcacc ataatccgaa tcagatctgc cttcgttgcc ttgtcccctt gcatcagagc
    51661 ccctgtccag gaagtcaact tcgtccgcta tcacttctgt tgcataatgc cttttgccat
    51721 cctgatcatc ccagtttctt gtctggagtc ttcctgaaac ggcaacaagc ctccccttct
    51781 ttatgaactt tccgcaatta tccgcagatt ttccaaatac aattatcggt ataaaatctg
    51841 cagttggttg accttccctt ttataggtcc tgtcaacagc gatagtgaat ttagctattg
    51901 gtgtttgatt atctgctgta tacctgattt caggatccct agtcaatctg cccaccaggg
    51961 caactttatt caacataatc acaccgccta aatttttttc ttttggattc ttttttccta
    52021 ctcttcaatt tttattatca tgtgccttaa aatctcttct gttatgccga atactctgtc
    52081 cagctctttt ggtacaacct gcgctgcttt atatttcata tatacatagt agccttcccc
    52141 gatatcgtct ataagataag cgagctttct tttgccccag tcatctatag cttcgatttc
    52201 accgttttct tcaacaattg ccttgaactt ctcctgaaga gcttttatac cttcttcatc
    52261 aacggtcggc ctgattatat atatggtttc gtacctgttc ataaaaattc acctcctccc
    52321 cacggactaa cggccctatc tgaatatgat agagcgagga tatttacgca attatagatt
    52381 ataacactat ataaagcttt aatcaatatt attctcttca ggtgctgcac tttttattac
    52441 ctttttaaca gatttttcaa acttaggcct cggcagcatc actattctgc cgcatttcag
    52501 gcattttatc ttgaaatccg ctcccaccct tatgacttcc cagtgattat cgccgcaggg
    52561 atgctgtttt tttgtttcca cgatatctcc tatgttgaat tgtttcgggt aatacatatg
    52621 catccatcct atcctatatt aattactttg ccggcagaat tcatcttttc aattattgaa
    52681 tacatatttc cgactatgcc gacctggagt ttatccttca actgatagaa atccaggcag
    52741 gtaccgcagc ttataatctc cacccctgag ttttccagct tcttaatatt ctccgccacc
    52801 tctgagccct ctgcggtaag cttaacacca ctgtttaaaa acatcacagc ctttggcagc
    52861 ggtgtggctt cggtaagtgc gtaagtatag ctctttatca gcacctttcc cagctcctct
    52921 gctccttggc ctaatttgtc cgaagttatt actattactg tgctgtcctc ttttgtttcc
    52981 gtactttctg ctcccgctgc ctttattatt tttatgtaat aacagccgct ttcgtcggat
    53041 atctcatatt tgcagcttaa attttcagcc aatcttgaaa cattttcctt tgcggtgata
    53101 ttgtctacaa tggttactac ctcgccctcc gtcatccctt caagggcctt tttggtgagt
    53161 attaccggtt gaggacacac aagccctctg gcatctattt ctttcatact tatcctcctc
    53221 actct
//
LOCUS       DAOQUR010000014        50409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_767_length_50409_cov_9.850161, whole genome shotgun sequence.
ACCESSION   DAOQUR010000014 DAOQUR010000000
VERSION     DAOQUR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 50409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50409
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_50409_cov_9.850161"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKI50_04725"
     CDS             complement(<1..591)
                     /locus_tag="PKI50_04725"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNT03046.1"
                     /translation="MPYDGSVVSSIVHELQNKLLNGKIEKVYQPEKDELIINVRGYGD
                     SFKLLLSASSAYPRVHLTEENKSNPASPPSFCMLLRKHLTGGRIVSIRQPEFERIIEI
                     DIDSTDELGYSTHKTLISEIMGRHSNIIFIDKTAGKIIDSIKRISFEISSVREILPGG
                     YYEYPPSAGKSDPNKATKESFIEGIKNTPGSIKAEKY"
     gene            986..1861
                     /locus_tag="PKI50_04730"
     CDS             986..1861
                     /locus_tag="PKI50_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNT03047.1"
                     /translation="MIKSMTGFGRGEFTQSTSTFSVDVRSVNHRYCDVSVRMPRAMSA
                     LEEKVREFVSERISRGKVDVYINYSTFGQNARVKLDTNLAKAYVDSLNTLKEIFEIND
                     DINLSLLSRFPDIMSLETVEQDMEELWLILKDALEMAFNALVEMRKREGERLKNDLLE
                     KVAVIKDYVECIKEKSSGIVDEYKNKLYDRIQELIKDIPIDENRLLTEVAVFADKASI
                     DEELVRLESHMDQFKKALNFNGPVGKKLDFIIQEMNREVNTIGSKITDLEILNNVISI
                     KTEIEKIREQVQNIE"
     gene            1874..2152
                     /locus_tag="PKI50_04735"
     CDS             1874..2152
                     /locus_tag="PKI50_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNT03048.1"
                     /translation="MGIKLINIGFGNIVSANRLVAIVSPESAPIKRIIQEARDRGMLI
                     DATYGRRTRAVIITDSDHIILSAVQPETVAHRLVDREAEDNEEVMAEE"
     gene            2170..2781
                     /gene="gmk"
                     /locus_tag="PKI50_04740"
     CDS             2170..2781
                     /gene="gmk"
                     /locus_tag="PKI50_04740"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004813319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNT03049.1"
                     /translation="MGNRGLLIVIAGPSGVGKGTICRELLRNYGENLELSISVTTRKP
                     RDGEAEGIHYFFRDMEEFFKMVENGELIEFAKVHDNYYGTPKRYVMEKLEEGKNVILE
                     IDVQGAMNVKKIFNEAVLIFVIPPTFADLKKRIEGRGTETKKDICKRLKNAYDEINHA
                     VNYDYIIVNDDIKDAAEKINCILIAEKSRLSRCNIDFEKFREE"
     gene            2783..3007
                     /gene="rpoZ"
                     /locus_tag="PKI50_04745"
     CDS             2783..3007
                     /gene="rpoZ"
                     /locus_tag="PKI50_04745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNT03050.1"
                     /translation="MIYPTLMSLLEKVDSKYTLVVAVAKRARQLVDGQPKLTNVNSNK
                     PVTIAINELYEEKITYERTKPVKISGDQNA"
     gene            3000..4196
                     /gene="coaBC"
                     /locus_tag="PKI50_04750"
     CDS             3000..4196
                     /gene="coaBC"
                     /locus_tag="PKI50_04750"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNT03051.1"
                     /translation="MLSGKKIVLGVSGGIAAYKACDIVSRLKKLNADVHVIMTASAEK
                     FVTPLTFQSLSLNQVAVDMFDTPNYWEIEHISLAKLADVIVIAPATANIIGKLANGIA
                     DDMLSTTIMATKAKVLIAPAMNTNMYENPVLQRNIKLLSELGYIMIPPTEGRLACGDV
                     GKGKMAEPAVIEKSIIDLLNSGQDMAGRTVLVTAGPTREAIDPVRFISNNSTGKMGYA
                     IARQAALRGAKVYLVSGPTILDTPIGVERYNVISAEEMYSKVMELFPKCDIVIKAAAV
                     ADYAPMTAYSQKVKKSGNQLELKLKKNPDILFELGKIKGDKILVGFAMETENLVENAA
                     EKVKKKNLDFIAANDLNEAGAGFAAETNAVKLIDREGNIENIPLKMKDEIADIILDKI
                     VNLKRT"
     gene            4258..6696
                     /gene="priA"
                     /locus_tag="PKI50_04755"
     CDS             4258..6696
                     /gene="priA"
                     /locus_tag="PKI50_04755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNT03052.1"
                     /translation="MGKMYAEVVVSNKCKETDRTYSYLVPENMKESIRVGSRVIVPFG
                     MGNKQIEGYVVGLKDSIDFKPSKIKSIGRLLDEKPVMSPKLVELAKWMKEKYLCYNID
                     AIQAIVPSPVRTKSSYSIELCGCEQNLEKLEKLNSNIILEIVEFLEENSGSAKLEEIR
                     EYFSDRKIDYYIKKLIEAEVVKKVQIIGSKIGEKKQKRLYLSEDKNIQIKKTASKQRE
                     VYELLLKCPGISPAKLKEACGDCGSAVKAMEKKGYVRIEETDEYRRVCSKTYAEKRHE
                     LTQDQNKALSVIRNCFSNGKDVLLFGVTGSGKTEIYLELIEEYISKGKDSIMLVPEIS
                     LTPQMVSRFKNRFGDNIAVMHSSLSEGEKYDEWRKIRDGIVKVAIGARSAVFAPFKNL
                     GIIIIDEEHEHTYKSGIKPKYLTREVAEKRCRLEGANLLLGSATPSIETYYRAQTGEL
                     GMAELGSRANGSPMPEVEVVDMREELESGNRTIFSRSLTDAILDNLKNKEQMILFLNR
                     RGHSTFVSCRQCGIVMKCPRCSISLTYHMKNEKLVCHYCGYERDNPAICPKCKSKNIK
                     YFGVGTQKLEKEFQRKFSAKDVLRMDADTTTKKNSHQEILDKFKRKEANVLLGTQMIS
                     KGLDFPDVTLVGVIAADTSLNLPDFRSAERTFQIIAQVSGRSGRGNKTGRVIVQTYSP
                     EHYSIEYARCHDYKGFYNKEVMLRKELGYPPFSSIANIMISGTAEEEVIAYANEISKF
                     LDEALKGYKDAEKLGPAAAAISRIKNRFRWQILVKSEREETLRKVLADLSEACGEGRS
                     NVSISIDLNPQSML"
     gene            7102..7557
                     /gene="def"
                     /locus_tag="PKI50_04760"
     CDS             7102..7557
                     /gene="def"
                     /locus_tag="PKI50_04760"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT03053.1"
                     /translation="MAIRTIRKDGDEILRKISRKVEVIDDRILTLLDDMKDTMKKAEG
                     VGLAAPQVGVLRRIVVIDVGEGLIELINPVIVYESGEQFEMEGCLSIPGIKGKVKRPE
                     KVIVRAQNRKGKTFEMTGTELLAIAFCHEIDHLNGILFTDKAIITDKED"
     gene            7580..8515
                     /gene="fmt"
                     /locus_tag="PKI50_04765"
     CDS             7580..8515
                     /gene="fmt"
                     /locus_tag="PKI50_04765"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNT03054.1"
                     /translation="MRIVFMGTPDFAVPCLRELIAHKHEIAAVVTQPDRPKGRGNKLA
                     ESAVKIFARESGISIFQPEKIKTGEFVQILRELAPEAIIVVAFGQILSQEILDIPPLG
                     CINVHASLLPKYRGAAPINWCIINGDKTTGVTAMYMDKGMDTGDIILKKEIEIGEDEN
                     AGELHDRLSELGASALSETMELLIEGKIERTPQNNEEATYAPIMTKELGRIDWSRDAK
                     DIRNLIRGTYPWPGAFSSYNGKIFKILRSQAMDDNTDGKNYGCIGSVHKDSIVVNCGG
                     GSLKILDLQFENEKPMSVEAYLRGHTIIAGTILGK"
     gene            8613..9302
                     /locus_tag="PKI50_04770"
     CDS             8613..9302
                     /locus_tag="PKI50_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNT03055.1"
                     /translation="MFYGYGLDPTFILILPAIILAAYAQSKVSSTFNKYLRVRNSYGH
                     TGYEVARRILDLNGLRDVPVELVRGRLSDHYDPRTRVLRLSQDVYNSNSLASVGVAAH
                     ECGHAIQHSEGYAPLVVRNAIAPVASFGSQAAWLFIIAGFIFSWVNLIDVGILLFSAA
                     VAFQVVTLPVEFNASSRAIALLEGNGLVPSDEIGPAKEVLNAAALTYVAATFAAVMQL
                     VRLLVLRGRRD"
     gene            9471..10817
                     /gene="rsmB"
                     /locus_tag="PKI50_04775"
     CDS             9471..10817
                     /gene="rsmB"
                     /locus_tag="PKI50_04775"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNT03056.1"
                     /translation="MKKIDLARDAAVRIVHRVLKEGAYSNIALKEELDKDRLLRVDKA
                     LVTEIVNGTLRNLTRIDWIISRFIKMKSIDPWIEDIIRCGIYQILFLDRIPDSAVCNE
                     SAELARKYGHEGTVRLVNGVLRNISRNKEKLEYPDKSENAASYLSVFYSHPEWIVKKW
                     LKDFGMEFTEALLKANNEKPPFTIRCNRLKIEKKGLLAILAEEGIEYTDGSYNAEAIH
                     IRGTSSIEAKESFRKGYYQVQDESSMLVAHIVDPKPGDKVLDICSAPGGKTTHMAELM
                     DNKGEIVARDIYRNKLRLVEENCDRLGISIVRTELFDAMVKDQGSIGKYDRVLLDAPC
                     SGFGVIRRKPDLRWKKDAKNLKELVDMQRRILELASEYVKPGGKLIYSTCTINRAENL
                     DVVRGFLSNNPQFQMESIIGQVPEKLENDSAHMGYLVLYPNVHGTDGFFIAKMEKR"
     gene            10824..11876
                     /gene="rlmN"
                     /locus_tag="PKI50_04780"
     CDS             10824..11876
                     /gene="rlmN"
                     /locus_tag="PKI50_04780"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNT03057.1"
                     /translation="MPDKKNIKDLGFDELEIYIKEHRLPGFRAAQVFEWIYKDTASFK
                     DMTNLPKNLIEQLEWDFHIGRAYIAAMQHSKTDNTRKYLICFEDGNAVECVLMDYSYG
                     KTLCISTQFGCRMSCEFCASTTGGLIRSMTSGEMLEEVMAVSRDIGERIGNIVLMGTG
                     EPFDNYDQVMKFIRTINHPKGLNIGQRHITLSTSGIVPRIYDFADEKLQCTLAISLHA
                     ADDEARSRIMPINRKYNIEKLMEACRYYINMTNKRITFEYALIKDVNDSVKDAHKLGT
                     LLKGMLCHVNLIPVNKVEGKNFSKASKERINSFKAALEGFGIETTVRRELGSEIDAAC
                     GQLRQKHINRQGVTNI"
     gene            11977..12744
                     /locus_tag="PKI50_04785"
     CDS             11977..12744
                     /locus_tag="PKI50_04785"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNT03058.1"
                     /translation="MRSAGRTDIGLVRKVNEDRFLCEKLEDIDSTYLYIVADGMGGHN
                     AGEVASSMAVQEVASYIKNSIGTLMAGDEEIKDLVRNAIMYANEKIYKTSILKSNCLG
                     MGTTLSMVLAKDSSIYIGHVGDSRIYLIRGKDISRLTEDHSLVAELVKRGTIKPEDAS
                     NHPQKNVITRALGTEYSIEPDVYQYDMQSGDLILICTDGLSNAVCEEDIAGVMNAASD
                     LNGACELLIEKAKEKGGYDNITAVAIQVCKGGEDNDR"
     gene            12734..14710
                     /gene="pknB"
                     /locus_tag="PKI50_04790"
     CDS             12734..14710
                     /gene="pknB"
                     /locus_tag="PKI50_04790"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HNT03059.1"
                     /translation="MIGRVLGDRYEILEKVGGGGMALVYKAKCRLLNRYVAIKVLRSE
                     FTEDEEFVKKFRREAQSAASLSHPNIVGIYDVGTENNSYYIVMEYIKGQTLKELIKSK
                     GTLGVEYSTNIAIQICYALEQAHKNNIVHRDIKSHNILIREDNSVKVTDFGIARAVSS
                     STITNTGNVIGSVHYFSPEQARGGYVDEKSDIYSLGVVMYEMLTGRLPFEGDSPIAVA
                     LKHIQEEPEPPSGINSRIPKAIEAIIMKCMEKEVSKRYNSASEIINDLRQSLVMPNGD
                     FVKKNRYTDESTRVLEPIKIIDKEAAVDIIEEPESKELNEGKEEKILIDDYSVDPIPK
                     VAEGKDKPVQNKRNVKLTIVAVFGGLLLALLLGGGILFVNSLFTVKDVEVPDIRNNSE
                     EEAREKLSALSLILEVTERVFNKDIPEGSIISQNPKPNEKNKITNPVKVVVSKGPRKV
                     VVPKLEGESYDKVKLILEGEGLVEGSFDQEYSEYPNGFVIRQSIAPGISVDEGTTIDY
                     VISIGPEKFIMPSYLGADIEDVKTDLIIKDLILGNISYDSNSEQPADTVLEQSIRPGT
                     EVSRKSVVDFVVNNYKPEPVPSSFKLRFTLPPELESMKVTVYKVQNEISSRIYESIHK
                     STDSPLEIPVSGVGNVIFEVYINDSLHESVAHNF"
     gene            14725..15606
                     /gene="rsgA"
                     /locus_tag="PKI50_04795"
     CDS             14725..15606
                     /gene="rsgA"
                     /locus_tag="PKI50_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNT03060.1"
                     /translation="MQKGVIVKGIGGFYYVDTGEGVYECRARGKFRLKNITPLIGDHV
                     EIDVDAGSLQGYIISILERKNQLIRPTVANIDQAVIVFAAKKPDINMVLMQKFLVYSE
                     YKNLNVIVCINKIDLDPDGHYLDVVRMLEGIPYKVITTSAKTKVGIEQLQEILKDKVS
                     VFAGPSGAGKSSLLNCIQPGLKLKIGDLSKKIDRGTHTTRHAELIKLDEGGMVADTPG
                     FTSLDIGELELSKLESCFPEFESHKNCQFVNCIHDKEPRCGIREAVLEGKIHNIRYDF
                     YISLLNELKSTGGRNYK"
     gene            15603..16262
                     /gene="rpe"
                     /locus_tag="PKI50_04800"
     CDS             15603..16262
                     /gene="rpe"
                     /locus_tag="PKI50_04800"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNT03061.1"
                     /translation="MIKIAPSILSADFANLERDIGTVERAGADWLHVDVMDGHFVPNI
                     TIGPAVVKSLRAKSSLVFDVHLMIDNPELYINDFAEAGADIITVHAEASVHLHRLLQM
                     IKAKGLKAGMALNPATPVNVLEHIIEDLDMVLIMSVNPGFGGQRFINSAYEKISKVRS
                     LIEKKNLSVDLQVDGGIGPDNIRQVVMSGANIIVAGSAIFNSGNIPETIRIMKEAGIS
                     K"
     gene            16262..16894
                     /locus_tag="PKI50_04805"
     CDS             16262..16894
                     /locus_tag="PKI50_04805"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNT03062.1"
                     /translation="MKTVIISGGEIKDYEFCSGIVRSADRVICADSGTRHAMNMNLTP
                     DVIIGDMDSSPAQYIDFFRKKGVEVIQYPKEKDKTDTHICVEYALDFSTEIILLGATG
                     SRIDHMIANISLLMLGIRRNIPMSIIDNKNCIRMIGDSITLKGNKGEFFSLIPYTGKV
                     EGLSTRGAHYELENAVMELGNPYGVSNYFEEETVSITIKKGFLLVIKSED"
     gene            17021..17176
                     /locus_tag="PKI50_04810"
     CDS             17021..17176
                     /locus_tag="PKI50_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03063.1"
                     /translation="MRYTYKRYAGCLCIILSVFLVSRFMPLFLWCFAVAAAVAMIGFL
                     LYNCFFM"
     gene            17194..17346
                     /locus_tag="PKI50_04815"
     CDS             17194..17346
                     /locus_tag="PKI50_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03064.1"
                     /translation="MSLLKRKKLTGFLAFCIGLGILFAVLVPAIGWVMFSAICLISIG
                     IFLIKY"
     gene            complement(17411..17602)
                     /gene="rpmB"
                     /locus_tag="PKI50_04820"
     CDS             complement(17411..17602)
                     /gene="rpmB"
                     /locus_tag="PKI50_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNT03065.1"
                     /translation="MARHCEVCGKGMISGNKVSHSNRHSRRIWLPNVRTVKAVVNGTP
                     QRLHVCTRCLRSGKVQRAI"
     gene            17899..18249
                     /locus_tag="PKI50_04825"
     CDS             17899..18249
                     /locus_tag="PKI50_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNT03066.1"
                     /translation="MSAIYKTQYGEVNITDDVLATISGLAATECYGLVGMASKNAKDG
                     LVELLKRENLAKGVKVTTEGEEIIVDLYIVVEFGTKISTIADNIISKVKYTVENLTGL
                     KVKKVNINVQGVRV"
     gene            18272..19909
                     /locus_tag="PKI50_04830"
     CDS             18272..19909
                     /locus_tag="PKI50_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNT03067.1"
                     /translation="MSTKNIDGIQLRNMFLSGANNLENNKGIVNSLNVFPVPDGDTGT
                     NMSLTMHLAVKEIIAVKSIEIQSVANAAANGSLMGARGNSGVILSQIWRGFAKALKNQ
                     KSMDVNLLANALTEGSNTAYKAIMKPTEGTILTVIRETAEYAMKVSENFEDNVLFWEE
                     IIEKANSVLRRTPEMLQVLKNAGVVDAGGKGLIYIFEGMYQYLKTGEMIELKAAADDE
                     EHDRPLFKEEEIVFGYCTEFFIKGRKLGLEKFRDEILPIGDSLLVVGDENLLKVHIHT
                     NNPGRVLELAMAQGELSRIKIDNMREQHNELLFNKEEHKAAEEAEQAPKPMERYGTIA
                     VAMGEGISSIFKDLGANEIIEGGQTMNPSTEDILNSVNKINAETIYIFPNNSNIILAA
                     NQAKSMSDKNVIVAATKSIPQGISALMALNHDKEIEENNKKIEKAITNVKSGLVTYAV
                     RNSNYDGINIEEGNVLGMVEGKISGVGNDILEVSHKVLDDMVDEDSSLISIYYGSEVN
                     EDEALKLKEEIGELYNNCEIELHFGGQPLYYYILSVE"
     gene            19927..21987
                     /gene="recG"
                     /locus_tag="PKI50_04835"
     CDS             19927..21987
                     /gene="recG"
                     /locus_tag="PKI50_04835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNT03068.1"
                     /translation="MNYTEDSITNLKGIGPVKARLFNKLGLYTIGDLLEYFPRGYKDK
                     SEITKIRDIMPDESCTVRVRAAGKAQEGRTRSRLIITKLTVFDDTGRLNAVWFNNRFI
                     KNAFREGDEVLMHGKVKRIGKDLVMEVPEYERLSDSESINLMRITPYYSLTEGLSQKD
                     IRKAVYAALKHAEKQADLFPEELRRKYGLAEINFCISNIHYPESMDKLKLARKRLIFE
                     EFFMLQSGLIHIKKLNSAGQKGIRFKKTSEIKEFIEHLPFKLTSAQERVFEEVSKDME
                     GSGIMNRLVQGDVGSGKTVIAALALFKCVRSGYQGTMIVPTEILAEQHMETLTNLLKN
                     TGVEIALLKGGMTGKEKQKVLEGIKEGKIDVVVGTHAIIQDSVSFDKLGLVITDEQHR
                     FGVRQRALLSAKGENPDMLVMTATPIPRTLSLIVYGDLDVSIIDEMPPGRKKVETYFI
                     DSAKKERLMNFVKKGLDEGRQAYFVCPLVEESDKLELKSAIEYSEELKNKYFRNYNVG
                     LLHGRMKADEKNEVMYRFREKEIHILVSTTVIEVGVNIPNAAYMVIEDADRYGMAQLH
                     QLRGRVGRGGHQSYCILISDTGNEIAQERLKYMTKTENGFEVAEKDMEFRGSGEVLGQ
                     RQHGLPGFKLADVFRDAKLLKLSKEAVEYIYENNKLSNEDYKKMKEILDQRFQKMLDE
                     ITLN"
     gene            22153..22362
                     /locus_tag="PKI50_04840"
     CDS             22153..22362
                     /locus_tag="PKI50_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HNT03069.1"
                     /translation="MPRNTTSNRTLIPEAKAALKTFRDQIATELGTDLTGYNGDKTSW
                     ECGKVGGEMVRRMIEQAERSMSGRR"
     gene            22851..23426
                     /gene="rsmD"
                     /locus_tag="PKI50_04845"
     CDS             22851..23426
                     /gene="rsmD"
                     /locus_tag="PKI50_04845"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNT03070.1"
                     /translation="MRVIAGEYKGRRLDPIEGRDIRPTSDMVKESLFNILGDAVIDSV
                     FLDLFSGTGGIGIEALSRGAKHVVFIDSGIKSIKVLRGNLNRLNIKDCAEVYNTDYGT
                     AINKLHEHGKSFDLIFIDPPYSMGMAESALKEIDNKNILTQSGLIVVEHDSKEDMPQK
                     VGKLFLYRFKKYGNTILSFYSIDEQSQQKEE"
     gene            23429..23905
                     /gene="coaD"
                     /locus_tag="PKI50_04850"
     CDS             23429..23905
                     /gene="coaD"
                     /locus_tag="PKI50_04850"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNT03071.1"
                     /translation="MKIAVYPGSFDPATNGHIDIIKRSGALFDRVIVAVLSNPSKNPI
                     FSVEERVDILKRTCGDIKNVEIDSFSGLLIDYCKSKNANVIVKGLRAVSDFEYELQMA
                     HMNKKLNQEIETVFIMTSSRYSYLSSSLVKEVAGFGGCIRGLVPEIIEKEIYERLK"
     gene            23978..24514
                     /locus_tag="PKI50_04855"
     CDS             23978..24514
                     /locus_tag="PKI50_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNT03072.1"
                     /translation="MEVMLLLDALEDILDKAPNLPLSSKSMINKEELQEIIKEIRIKL
                     PDEIKQAQWIKEERQKILIEAKKEAENLRKECEEKLNKVKEERQRILAEAEKEAEILK
                     KEADKKVQELIDESDIIKKANEQAKELIIAAQIDAKKIRLGSKAYADELLAELEKHTE
                     KIIETIRNNRDELKNYKS"
     gene            complement(24560..25753)
                     /gene="ylbJ"
                     /locus_tag="PKI50_04860"
     CDS             complement(24560..25753)
                     /gene="ylbJ"
                     /locus_tag="PKI50_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HNT03073.1"
                     /translation="MKKGTISTFAYSFIFCILVTFIIRYPGEAFAASLDGLKLWFNVV
                     CPALLPFFICVEILIGLGIVRLFGSAFRPLMGPVFNVPGEGSFDFFMSISSGYPVGAK
                     ITAELYSKNLCTLAEGQRLLSLCSTSGPLFIIGAVATGILNNPALGSFIAIAHYLSAI
                     TIGFAMRFYGKENPRIREKYISNPIAEMMEYRRRDGRTFGMLMRDAVKNGIDLILMIG
                     GFIIFFSVITGMLRISGFLEWFSLSLSRAIPLKSITPDIISSLLIGLLEVTNGAKSCA
                     SLRVPLIYKAAITSFMIGFGGLSVNAQVMSIISGTDLKLGIYFITKLFQGIAAAFYSY
                     VLVAWFSDLQVFNLPAESFVQNTILEYSFLRVLVTSAANMLIILLFMSAVHIKGTWGR
                     FSCPK"
     gene            complement(25833..27083)
                     /locus_tag="PKI50_04865"
     CDS             complement(25833..27083)
                     /locus_tag="PKI50_04865"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNT03074.1"
                     /translation="MMKTVGIVSEYNPFHNGHKYHIRAAKEACGADYAVCIMSGSFVQ
                     RGEPSIFDKWSRSKSAVMNGADLVIELPVVYACQPAEIFAAGAVKILDSLGVVDHICF
                     GSELGDTGALHRLANLLQNEPEAFKHLVKGQLGRGVSYPKAVSNALSHYASDDIHYAS
                     DDILKNPNNVLGIEYIKALKSLNSSITPVAVKRIVSGYNDNTIAGSIASATAIRNEIK
                     EGGISDKVRMSLPGSSIDIISVNLRMNRHPVYMNDFSDILLYKLRTMEKSELLKYPNV
                     KEGIENRLKKYAAASTNCEDLIEAVKTKRYTRTYIQRLLCHVLLDIKNADISMFKSRE
                     CPSYVRVLAFNNNGKKLIKMIMDCCPYPVISKVADFRCTDKRIIKMFSYDIQATDIYN
                     LAYRTSEFRKAAEDYFTSPYYHRE"
     gene            27289..28494
                     /locus_tag="PKI50_04870"
     CDS             27289..28494
                     /locus_tag="PKI50_04870"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNT03075.1"
                     /translation="MKILVINCGSSSLKYQFIDMENEEVIAKGLAERIGIDGSVLNHQ
                     PKGFDKIRIERPMPTHKEALQTVVDALRDKEYGVIKDMGEITAVGHRVVHAGEKFAYS
                     VLLKEEVMDALKECISLAPLHNPPNIMGIEACKQIMPEVPMVGVFDTAFHQTMPKKSY
                     IYPIPYEYYEKYRIRRYGFHGTSHKYVAERAAAMLGKPVEELKIITCHLGNGASITAV
                     KNGISVDTSMGFTPLEGLAMGTRSGNIDAAIIKFLMEKENISVAKADEILNKKSGVLG
                     ISGVSSDFRDIEEAANKGNERAQLALDVYVHYVKKYIGAYAAVMNGVDAIVFTAGLGE
                     NSASMRSSICKDMSFLGVELDEAKNNVRGKETDVSKDGSKVRVLLIPTNEELMIARDT
                     AELAGKAKK"
     gene            28640..29146
                     /locus_tag="PKI50_04875"
     CDS             28640..29146
                     /locus_tag="PKI50_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNT03076.1"
                     /translation="MELNFAGISKSEGVKERFEIIEYPIDSTIVFCGENLNIVSPITV
                     KGSAVNYEGKISVDLTITATVERTCSRCLEIFTEEVQADSNYVFVKDIADDKEDYYIY
                     RGDKADITDLVIGDIAAKLAMKPLCRANCKGLCTVCGKNKNSIDCQCKSEEVDPRMQA
                     LSKLLDRK"
     gene            29160..29339
                     /gene="rpmF"
                     /locus_tag="PKI50_04880"
     CDS             29160..29339
                     /gene="rpmF"
                     /locus_tag="PKI50_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNT03077.1"
                     /translation="MGLPKRRHSQSRRDKRRANWKLALPGLVACPQCHEPKLPHRVCK
                     SCGYYKNRTVIKTEE"
     gene            29652..30215
                     /gene="fapR"
                     /locus_tag="PKI50_04885"
     CDS             29652..30215
                     /gene="fapR"
                     /locus_tag="PKI50_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HNT03078.1"
                     /translation="MKQGGISKKERQASLLDRIKTDPFLTDEELAESFNVSIQTIRLD
                     RLELGVPELRERIKSVAEDNYTKVRSLHGKEIFGELVDLNLGVSGISILETDNNLAFE
                     KTNVVKGQYIFAQAESLAIAVIDANVALIGVANIKYKNPVFAGDKLIAKAEVVRTRGN
                     KYFVWVFIRVKQAEVFRGKFILVSFSN"
     gene            30366..31382
                     /gene="plsX"
                     /locus_tag="PKI50_04890"
     CDS             30366..31382
                     /gene="plsX"
                     /locus_tag="PKI50_04890"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNT03079.1"
                     /translation="MRIAVDVMGGDNAPGAIIEGSILALKEYGTELYLIGLRDEILKY
                     LGEEYKNHQKVNIIEASEIINADEAPVSAIKQKKDSSMVIGLKLVKEGQCDAFVSAGS
                     TGAFLAGSLLKVGRIKGIDRPALSPLLPTLKGSCMIIDAGANADSKPKNLQQYAVMGS
                     IYMEKVMGIKKPKVGLLNIGAEASKGNELTKQSYTLIKNTGVNFIGNTEARDIISGVC
                     DVCVCDGFAGNVLLKTTEGVAMTLFKLLKNEFMKSLKTKLGAMLLMDSLKNIKKSFDY
                     TEVGGAPFLGINGIMIKAHGSSDAKAIKNAIRQAKMLYDNKCLDTIMEEIIKLGVEDT
                     DEQA"
     gene            31369..32376
                     /locus_tag="PKI50_04895"
     CDS             31369..32376
                     /locus_tag="PKI50_04895"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNT03080.1"
                     /translation="MNKLRNAGIIGTGSCLPDKIVTNQDMEKTVDTTDEWIRTRTGIS
                     ERRMTDENTATSDLATIAAMRALASANLSPEEIDLIIVSTVTPDMMFPSTACIVQKNI
                     GAVNAAAFDLEAACAGFLYGLSIADSFVRAGSFNKILVIGAETLTKILDFTDRNTCVI
                     FGDGAGAAIVSEVPEGYGIMSTYIGADGRGGDLLKVPAGGSRIPSSVESVQNRLHYVK
                     MDGKEVFKFAVKIMGDAAEIALGKCGLTKEDVDFFIPHQANIRIIEASVKRLKIAHEK
                     VYINLDKYGNMSSASVAIAIDEANRKGLLKDGDTIVLVGFGGGLTWGSAVLKWKSIRR
                     D"
     gene            32378..33328
                     /gene="fabK"
                     /locus_tag="PKI50_04900"
     CDS             32378..33328
                     /gene="fabK"
                     /locus_tag="PKI50_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HNT03081.1"
                     /translation="MFKTSICEILDIEYPILQGGMAWVATAELAAAVSNAGGLGIIGA
                     GNAPGSYVRDEIRKVKKLTDKPFGVNIMLLSPFVDEVIDIVYEEKVPVITTGAGNPGK
                     YIARFKNIGTKVIPVVPAVALAKKMEQEGVDAVIAEGTESGGHVGELTTMALVPQVID
                     AVKIPVIAAGGIADGRGLVSVLALGAQGVQMGTIFVCASECTAHENYKKAIIKAGDRG
                     TVVTGKATGHPVRILKNKLAREFEKLEKASAAVEEYEKLGVGRLRAAVVEGDADYGSV
                     MAGQIAGLVKEIKPAKDIIEDILNEANEVISSINGLVKEE"
     gene            33330..34280
                     /gene="fabD"
                     /locus_tag="PKI50_04905"
     CDS             33330..34280
                     /gene="fabD"
                     /locus_tag="PKI50_04905"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNT03082.1"
                     /translation="MPKIACIFPGQGAQYPGMGKELAQNYKEAMNIFDLASERLGIDM
                     KKLCFEGDEEELKKTENTQPSILTVSIALLEVLKLKGIEPQIAAGLSLGEYSALVASK
                     AISFADAVALVKKRGKYMQEAVPAGEGTMAAIMGIERESVMECLKMASGYGVVEAANF
                     NCPGQVVIAGHTSAVEKACSILKERGARKAIMLPVSAPFHSSLLKPAGEKLAAELENV
                     NISDNELPVVSNVNAQVIMNKYEIKRCLIEQVSSSVLWEDSIRRMIDLGVDTFIEVGP
                     GRTLSAFVKKIDKSLYVHNVENIETMENTIVSIRERLECN"
     gene            34271..35014
                     /gene="fabG"
                     /locus_tag="PKI50_04910"
     CDS             34271..35014
                     /gene="fabG"
                     /locus_tag="PKI50_04910"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNT03083.1"
                     /translation="MQLKGKTAVVTGGSRGIGRAIALELASCGANVVVNYTRNSKAAD
                     EVVAEIEAMGSSGMAVKADVSIASEVENLVNEVLKTFGSIDILVNNAGITRDNLIIRM
                     TEKEFDEVINTNLKGTFICTKAVSRVMIKQKSGKIINVSSVVGIVGNAGQSNYAAAKA
                     GLIGFTKSMAKELAKRGINVNAVAPGFIQTDMTSVLPDNVKEEFLKSIPLMRIGKPED
                     IAKTVLFLVSEYSDYITGQVINIDGGMVM"
     gene            35159..35386
                     /gene="acpP"
                     /locus_tag="PKI50_04915"
     CDS             35159..35386
                     /gene="acpP"
                     /locus_tag="PKI50_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNT03084.1"
                     /translation="MIFEKVQEKVAEQLGIEPEEITLESSFINDLGADSLDIVELLMA
                     LEEEFDIEIPDEEAEKLSTVGDVVEYIKSNK"
     gene            35484..36725
                     /gene="fabF"
                     /locus_tag="PKI50_04920"
     CDS             35484..36725
                     /gene="fabF"
                     /locus_tag="PKI50_04920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNT03085.1"
                     /translation="MNKRRVVVTGIGVISPIGTGREKFFKSLKEGVSGVGHITRFDTE
                     GFDTKIAAEVKDFDPSGYFDRKECRRMDRFSQYAIAASKMAAEDSNINIESLDSSRFG
                     VCIGSGIGGMETMEAQYDILREKGPGRVSPFMIPMMISNIAAGNISIALNAKGPSITV
                     VTACASATNAIGEAFKIIQRGDAEIMVTGGTEASITPLSLAGFCSMKALSTRNDDPKA
                     ASRPFDKDRDGFVMGEGAGMLIIEELEHALKRGAVIYGEIAGYGATGDAYHITAPAPD
                     ADGAYRAMLMAVNDAGINACELDYINAHGTSTDLNDKLETLAIKRLLGEEAKKVAVSS
                     TKSMTGHLLGAAGAIEAIACLMAINEGVIPPTINYTTPDEECDLDYVPNKAVNRKVDW
                     AMSNSLGFGGHNASIILKKYQ"
     gene            36840..37559
                     /gene="rnc"
                     /locus_tag="PKI50_04925"
     CDS             36840..37559
                     /gene="rnc"
                     /locus_tag="PKI50_04925"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNT03086.1"
                     /translation="MSVIDEKRLKQLNEFESIIGYSFKNISILNNALTHSSYVASRSD
                     HHEHNERLEFIGDAILDMVISLYLYKKCKNLTEGSLTRFRAGIVCEQSLYNASNNLKL
                     GEYLLLSKGEENTGGRKRVSILADAFEAVIAAIYLDGGIKKAKEFIMKNLETTIKNAV
                     ENKLFSDYKSFLQEYVQKNNLGKLSYKLVREEGPDHDKTFEVALYLNNGIIGRGEGRS
                     KKDAQQSAARDAIAGLGVRNV"
     gene            37552..38622
                     /locus_tag="PKI50_04930"
     CDS             37552..38622
                     /locus_tag="PKI50_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT03087.1"
                     /translation="MFKKHYIIPIFVPHKGCPHDCIFCNQKKITGQIEKVTEKDVKDK
                     IEDYLDMIHEGGSHIEVAFYGGSFTAIPLEYQEQLLDAAFVYVKAGKITGIRVSTRPD
                     CIDARISENLRKYGVRTVELGVQSMDEEVLKLCNRGHSPGDVTNAVGILKEFGFSVGV
                     QMMIGLPGDTEEKAVKTAKALIALKPDIARIYPALTIKNTYMEEMFIRGEYQPLTLDE
                     AVELSKKLLILFESNGINVIRIGLQPTENINSGKDVVAGPFHPSMRHLVESLIYRDML
                     AYLLEKEGEKKKITLGINPKNVSELVGHKKSNIDYIKSKFFIDKIEIFQDNNVEAGTM
                     VLNNGKNSVIMSKKYYYSILSK"
     gene            38691..38951
                     /locus_tag="PKI50_04935"
     CDS             38691..38951
                     /locus_tag="PKI50_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNT03088.1"
                     /translation="MEVLKVSAHSQPKSVAGALAAVLRDRATAEIQAVGAGAVNQAVK
                     AIAITRGFVAPNGIDLVVVPAFSEIQIDGEDRTAIKFIVEPR"
     gene            39095..42655
                     /gene="smc"
                     /locus_tag="PKI50_04940"
     CDS             39095..42655
                     /gene="smc"
                     /locus_tag="PKI50_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNT03089.1"
                     /translation="MYLKQLEVHGFKSFAEKIELSFGNTINAIVGPNGSGKSNISDAI
                     RWVLGEQSVKALRGNKMEDVIFAGSDSEKALGFAEVSLCIDNSDRKIPVDYTEINIMR
                     RMYRSGESEYFINKTSCRLKDIYELFMDTGIGRDGYSIIGQGRIDEILSNKSEDRRNV
                     FEEAAGIVKYKTRKHEAEKKLEQTNQNIVRLGDIIEELESQLGPLKEQAEVAKRYRSI
                     MEELKSIEINIMLNSIDKFRSRLSNAKQEAENIQSLIDAKNSSARQLEHKQNELKRIL
                     NDIETSIDMLSEDIHHLENSKEKMEGESNVLQVRINNLEENTLKLGKSGEELENELTE
                     SRQGIEQNRLKLAELNTAYIEVYGKINERTADFESNFKAINDLESDLSDKQQKFISLI
                     NNYSELKSKINSTNIFIENMNKRKSQVEGDVNLLELQQQDKKKQYDKSLEELDDFGRK
                     IEDCKTQIEEIKGKKQECESEILLLEDKAGKLKAEKEAMFSRLKALDDMERDHGGYSR
                     AVKVLMAEYDKVTKGFCGVVGEMIKVPSKYVTAIEIALGGSVQSIITNTENDAKLLIE
                     YLKEHKTGRATFLPISSVKPRFFNKAEEKLFNMKGFVGIASDLIEYDLKIDSIMKNLL
                     GRVAVVEDIDAAIKLAREAGYDYKIVTLSGEVINAGGSITGGSLQSSSSSVLTRKSEI
                     EALKVKHKEKQQELDELSRKLGELKKQAADMGADIEFKTEILHSLQIDINNYRNRCDN
                     LKNEIQVIQDRISINLKDLGDLEKEYNETNKIISENKEKAEAVEKEKEAVEAQISELQ
                     EQMKQFKEVRDEYNDEITELKIKLASVEHNKKTVENSIKSMGERVTELEEEIKSNNAE
                     IERQQAEIKALSYDLEEKKTIIERTVREAEEKHKLHNDKEKEKSAICEDLSKLEADIK
                     NYSNDIAVLQADLHKAEMQHARTEIELESIEMRLLETYEMNYSKAESYRDTSLSLTWA
                     AKHCEELKNDIREMGTVNVNAIEEYEKLKERHTFLSVQVEDLSKARENLIAVIAEIEE
                     NMKLQFITEFNKINENFNIVFKQLFGGGHAELVLTDMDDVLGSGIEIIAKPPGKKLQN
                     LLLLSGGERALTAIALLFGILKMKPAPFCVLDEIDAALDDANVDRFAEFLVSFSATTQ
                     FIVVTHRKGTMECADCLYGVSMEDSGVSKLVSVRLEDKVS"
     gene            42816..43883
                     /gene="ftsY"
                     /locus_tag="PKI50_04945"
     CDS             42816..43883
                     /gene="ftsY"
                     /locus_tag="PKI50_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNT03090.1"
                     /translation="MAGLFKKLFNRGQENEQEELQAEEQEQLTDEEIEAINEEEIENE
                     ETVENEQSSIFSRLKNGLAKTRNNITGKLNGVLASFKSVDEELFEELEEILITSDVGV
                     STTLMILDKLRSRVKEQNIKDPSEVKELLKQEILAIMGESTERLGEQTPAVILVIGVN
                     GVGKTTSIGKIASKMKKSGKKVIIAAADTFRAAAIDQLEIWGKRIGVDVIKHQEGADP
                     AAVIFDAINSAKSKKADILICDTAGRLHNKKNLMEELKKISRIIEREFPEAYKETLLV
                     LDATTGQNAISQAKLFKEAADINGLVLTKLDGTAKGGIIIAIKSELDIPVKLIGVGEK
                     VKDLQEFSASDFVNALFEEDE"
     gene            43978..44334
                     /locus_tag="PKI50_04950"
     CDS             43978..44334
                     /locus_tag="PKI50_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HNT03091.1"
                     /translation="MYDKLVQIALLFDFYGQLLTEKQIEIVDMYYNNDLSLGEISEQQ
                     GISRQGVYDTLKRAERTLYQYEEKLGLVDRFLKQKQNMEKIIEMLEELITEDGYDRKN
                     IKDKINEIKEYIGNII"
     gene            44404..45744
                     /gene="ffh"
                     /locus_tag="PKI50_04955"
     CDS             44404..45744
                     /gene="ffh"
                     /locus_tag="PKI50_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNT03092.1"
                     /translation="MAFEGLAEKLQNTLKKLRGAGKLSEKDVKDAMREVKMALLEADV
                     SFKVVKEFITKVTERAVGQEVLDSLTPGQQVIKIVNEQMTELMGNTNSKIIFSPKPPT
                     ILMMVGLQGAGKTTTCGKLGLLLKKQGKNPLLVAGDVYRPAAIKQLQVVGSQVGIEVF
                     AMGDKVSPVDISKAAAAHALKNGNDVVIIDTAGRLHINEELMDELADIKENVKPTEIL
                     LVVDSMTGQDAVNVAESFNEKLGIDGVILTKLDGDTRGGAALSVKAITGKPIKFAGMG
                     EKLNELEPFHPDRMASRILGMGDVLSLIEKAQSAFDEKKAVELERKIRTQQFTFDDFL
                     DQLQQMKKMGPLSSVLEMLPGMNPSKLKGMEVDDKELVKVEAIIRSMTKKERHNPDII
                     NGSRRKRIAAGSGTSIQDVNRVMKNFEQSKKMMKQFAGMEKDMKKGKMKMPFFR"
     gene            45788..46036
                     /gene="rpsP"
                     /locus_tag="PKI50_04960"
     CDS             45788..46036
                     /gene="rpsP"
                     /locus_tag="PKI50_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNT03093.1"
                     /translation="MSVKIRLRRMGAKKAPFYRLVVADSRSPRDGKFIEEIGYYNPIT
                     EPAEIKIDAEKAKKWLDCGAQPSDTVRSLLKKSGIIEG"
     gene            46072..46299
                     /locus_tag="PKI50_04965"
     CDS             46072..46299
                     /locus_tag="PKI50_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNT03094.1"
                     /translation="MKELVEVIAKALVDHPEMVKVRETENERSIVLELTVAPEDMGKV
                     IGRNGRIARAIRTVVKSSSARLDKKVIVEIL"
     gene            46403..46906
                     /gene="rimM"
                     /locus_tag="PKI50_04970"
     CDS             46403..46906
                     /gene="rimM"
                     /locus_tag="PKI50_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907560.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNT03095.1"
                     /translation="MLEYLSIGQIINTHGLRGAVKVYPLTDDISRFKKLKEVYVEEND
                     GIVKYKVESVKFLSSAVAVKLKGIDSEEAANKLRGFYIKVDRKSAVKLPKDTYFICDL
                     IDLEVYDEKGLLIGTVKDVLQTGSNDVYVIESSGKDILIPALKDIVKKIDLENKKILV
                     EMPEGLI"
     gene            46907..47644
                     /gene="trmD"
                     /locus_tag="PKI50_04975"
     CDS             46907..47644
                     /gene="trmD"
                     /locus_tag="PKI50_04975"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNT03096.1"
                     /translation="MKIDILTLFPEMFEAVLKESIIGRALEKQLIELEFHNIRDYSDN
                     KHRKVDDYPYGGGLGMIMQCQPIFAAVEAISAKLGEKPHIVLMSPQGRTFDQAKAEEL
                     STHKYLTILCGHYEGIDERVNEALVDEEISIGDFVLTGGEIAAMAVVDATCRLIPGVL
                     KEDASYTEESFNRGLLEYPQYTRPPVFMGMPVPEILLSGHHENIEKWRRYQSLKRTWE
                     RRPDLLAESELSEEDRRLLDKIKEECK"
     gene            47792..48136
                     /gene="rplS"
                     /locus_tag="PKI50_04980"
     CDS             47792..48136
                     /gene="rplS"
                     /locus_tag="PKI50_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNT03097.1"
                     /translation="MMDLIKVIESEQLRNDIPVFNVGDTVKVHVKVKEGNRERIQVYE
                     GVVMKRQGGGLSETFTVRRISYGVGVERTFPLHSPKLDRIEVVRKGKVRRAKINYLRG
                     RVGKRAKIKEIR"
     gene            48181..49038
                     /gene="ylqF"
                     /locus_tag="PKI50_04985"
     CDS             48181..49038
                     /gene="ylqF"
                     /locus_tag="PKI50_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNT03098.1"
                     /translation="MNIQWYPGHMAKAKRKIAEELKLVDVVIELLDARIPMSSRNPEV
                     DEIVGNKKRIIVLNKSDLADSSVNAKWLVYFNKENTRAILVNSLKGNGLKDVLSASRQ
                     LMKEKLDRLKSKGLLVKTTRALIIGIPNVGKSTFINRLAGRSVAQTGDRPGVTKSKQW
                     IKVSNELELLDTPGILWPKFEDKRAGMYLAFTGAIKDEILDVNELAQNLLQVLAEKFP
                     EKLKSRYKLEDIPEGIAPEELMKSIGRKRGCIIAGGEVDVLRASAMLLDEFRGGKIGA
                     ISLESPEDF"
     gene            49209..50150
                     /locus_tag="PKI50_04990"
     CDS             49209..50150
                     /locus_tag="PKI50_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNT03099.1"
                     /translation="MPKKNDDYISVLGGCNMDIIGFPYKNFVPEDSNPGRIKAALGGV
                     GRNIAENLVCLGIPTKLISAIGDDIYGKIILEHADNIGLDMKNSLVSNQTTTSTYLAI
                     LDEKGDMKAAVSNMDVLDEITIEFIQEKKQVIENSKICVIDTNMPTDIINYVLDNFKN
                     TDFFLDTVSGTKAQKVKNNIGAFHTIKPNRIEAEILSDIKIDSLGDMIKASEYFLSRD
                     VKRVFITLGAEGVFYNDGNVHKLIPSPKIKVINATGAGDAFLAALAFCRFKNYGIDES
                     TRFSMAAAILALSCEKTINPNISEKNVIKKMKEIGLY"
BASE COUNT        16754 a         8501 c        12637 g        12517 t
ORIGIN      
        1 gtacttttca gcctttatac tgcccggagt atttttaata ccttcaatga agctttcctt
       61 tgtggcttta ttgggatcag atttgccagc cgaaggaggg tattcgtagt atcctcccgg
      121 cagtatttct ctgacgctgc ttatttcaaa gcttatcctt ttaatactgt ctattatttt
      181 gccggcagtc ttgtcaataa atataatgtt gctgtgacgc cccattattt ctgagataag
      241 ggttttgtgc gtgctgtacc ctaattcgtc tgtagagtct atatctattt caataattct
      301 ttcaaattca ggctgcctga ttgatactat acgtcctcct gtaagatgct ttctcagcag
      361 catgcaaaag gaagggggag atgcaggatt ggatttgttc tcttcggtaa gatgtactct
      421 tggataggcg gagctggcac ttaacaaaag cttgaagctg tctccatagc ccctgacatt
      481 tattattaac tcgtcctttt caggctgata tactttttct atttttccat tcagcagctt
      541 attctgcagc tcatgcacaa ttgagctgac caccgaaccg tcgtaaggca taagaacacc
      601 tcctgttcac tccattgggt tcatctaaat attatatcac ttgagcagct actttactat
      661 taacaaaaat atgtttgcgc aaaagaggaa gaatgcgggg acatggggga cgggacacgg
      721 ggacgtttcg cgtgtcccac tggaagtggg acaggggaaa cgtccccgtg tcccgtcccc
      781 gtgtccccgt gtctcataag tgcagttgat gataattaac gaatttatca gaaaatagga
      841 gcaatatcaa ggaaatcaaa aaaaacaaaa atttttagct taatatatgt ttacaaacaa
      901 gtttatatgt tataataatt ccaaaatgca actaaaatat ataaaatgtc cggatataca
      961 acagcgaaac ggaaggatga tgagtttgat aaagagcatg acgggctttg gaaggggaga
     1021 attcacacag agtaccagca ctttttccgt tgatgtaagg tcggtaaatc acagatactg
     1081 tgatgtgtct gtaagaatgc caagagcgat gtccgcattg gaggagaagg taagagaatt
     1141 cgtcagcgaa aggataagca ggggaaaggt ggatgtgtac ataaactata gtactttcgg
     1201 gcagaatgca cgggtaaagc ttgacaccaa ccttgcaaag gcatatgtgg attcacttaa
     1261 tactctgaaa gagatatttg aaataaatga tgatattaat ttatcgctgc tttcaagatt
     1321 ccccgatatt atgtctttgg agactgttga gcaggatatg gaagaactct ggctgattct
     1381 caaagacgca ttggaaatgg cattcaatgc attggtggaa atgagaaagc gggagggcga
     1441 aaggctcaag aatgacctcc tggaaaaagt cgcagttata aaggattatg tagaatgcat
     1501 aaaggaaaaa tcatctggta tagttgatga atataagaat aagctatatg atagaataca
     1561 ggaactgata aaagatattc ccatagacga aaacaggctc ctgaccgaag ttgctgtttt
     1621 cgcagacaaa gcaagtattg atgaagagtt agtcaggctt gaaagtcata tggatcagtt
     1681 taagaaagca cttaacttca acgggcctgt cggcaagaag ctggatttca taatacagga
     1741 gatgaacaga gaagttaata ccattggttc aaaaattacc gatttggaaa tattgaataa
     1801 tgtgataagc ataaagacag aaattgaaaa aattagagag caagtacaaa atattgaata
     1861 acggagggtt aaaatgggta tcaagcttat aaacataggt ttcggcaata ttgtatcggc
     1921 aaacagatta gttgcaatag ttagtcctga atcagctcca atcaagagga taatacagga
     1981 ggcaagggac agaggcatgc ttattgatgc tacatacggc agaaggacga gagctgtaat
     2041 aataaccgac agtgaccata taattctttc tgcagtgcag cctgaaactg ttgcacacag
     2101 gcttgtggat cgcgaagcag aagacaatga agaggttatg gccgaggaat aaagaagggt
     2161 aggataaata tggggaatag gggtctgctt attgttattg cagggccttc aggcgtggga
     2221 aaaggtacca tatgcagaga attgcttcga aattacggcg aaaaccttga actttctatt
     2281 tcagttacaa ccagaaagcc cagagatgga gaagctgaag ggattcatta ctttttcagg
     2341 gacatggaag agtttttcaa aatggttgag aatggagaac tcatagaatt tgcaaaagta
     2401 catgataatt attatgggac tcccaagaga tatgtcatgg aaaagcttga ggaaggaaag
     2461 aatgtaatac tggaaataga tgtgcagggt gccatgaatg tcaaaaagat ttttaatgaa
     2521 gctgttctta tttttgtaat tccgcctacc tttgcagatt tgaagaaaag aatagaagga
     2581 cgtgggacag agacaaaaaa agatatttgt aaaaggctga aaaacgctta tgatgaaata
     2641 aaccatgccg ttaattatga ttatattata gtaaatgatg atataaagga tgcagcagaa
     2701 aagataaact gcattcttat tgcagaaaaa agcagattgt caagatgtaa tatagatttt
     2761 gagaaattca gggaggaata acatgatata tcctacttta atgtcattgt tggaaaaggt
     2821 tgatagtaaa tatacacttg tggttgctgt tgcaaaaaga gccagacaat tggtggatgg
     2881 ccagccaaag cttacaaatg taaattcaaa taagccggta acaatagcaa taaatgaatt
     2941 atatgaagaa aagattacct atgagagaac caagccggta aaaatatcgg gggatcaaaa
     3001 tgcttagtgg aaagaaaata gttcttggtg tatcgggcgg aatagcagca tataaggcct
     3061 gtgacatagt aagcagactc aagaaactga atgcggatgt tcatgtcata atgacggctt
     3121 cagccgaaaa atttgttact cccctaacct tccagtccct ttcattaaac caggtggctg
     3181 ttgatatgtt tgatacgccg aactactggg agatagaaca tatatccctg gccaagctgg
     3241 cggatgtaat tgttattgct cccgcaaccg caaatataat tggaaaactt gcaaacggta
     3301 ttgcggatga tatgctttcc accaccatca tggccactaa ggcaaaggtt ctgattgcac
     3361 cggcgatgaa taccaatatg tacgaaaatc ctgttcttca aaggaatatc aagcttcttt
     3421 cggaattggg atatattatg attccgccga cggaggggcg ccttgcctgc ggtgatgtgg
     3481 ggaagggaaa gatggctgaa cctgctgtta ttgagaaatc aattattgat ttgctgaact
     3541 ccgggcagga tatggcaggc agaactgtcc ttgtgactgc gggacctaca agagaagcta
     3601 ttgatcctgt aaggttcata tccaacaatt ccacaggaaa aatgggatat gcaatagcca
     3661 ggcaggcagc attaagggga gcaaaggttt atttggtctc cgggccaacg attcttgata
     3721 cgccgattgg tgtagaaaga tataatgtca tatccgctga ggagatgtac agcaaggtca
     3781 tggagctttt cccgaagtgt gatatagtaa taaaggctgc tgcagtagcc gactatgcac
     3841 caatgaccgc atacagccag aaggtgaaga agtcgggaaa ccagcttgaa ctgaagctaa
     3901 agaagaatcc tgatatactt tttgagcttg ggaaaatcaa aggcgacaaa atacttgtag
     3961 gttttgccat ggagactgaa aaccttgtag agaatgccgc agaaaaagta aagaaaaaaa
     4021 atctggattt tatagcagcc aatgatttga acgaggcagg agcgggtttc gcagcggaaa
     4081 caaatgcggt aaagcttatt gacagggaag gaaatatcga gaacattcct cttaaaatga
     4141 aggatgagat tgcagatata attcttgata agatagtaaa tttaaagcgt acatagcagt
     4201 acgcttttaa gttatgtgca gcactgaaga taccggattg cattggggga aatcaaaatg
     4261 ggaaaaatgt acgcagaggt cgtagtgtcc aataagtgca aagaaacaga cagaacttac
     4321 agctatctgg tgcctgaaaa tatgaaggag tcgatccggg taggcagcag ggtaattgtg
     4381 ccttttggca tgggtaataa gcagatagag gggtatgtgg tgggcttgaa ggacagtata
     4441 gactttaagc cctcaaagat aaagtccata ggcaggctgt tggatgagaa acccgtaatg
     4501 tcccccaagc ttgtagaatt ggcaaaatgg atgaaggaaa aatacctgtg ttacaatatt
     4561 gatgccatac aggccatagt gccttctccc gtaaggacaa aaagcagtta cagcattgag
     4621 ctttgcggct gtgaacaaaa tttagaaaag cttgaaaaat tgaattcaaa catcattctg
     4681 gaaatagtgg agtttcttga ggaaaactcg ggaagtgcca agctggagga aataagagaa
     4741 tatttcagcg acaggaaaat agattattat atcaagaagc ttattgaagc agaggtagta
     4801 aaaaaagtcc agataatcgg aagcaaaatt ggggaaaaaa agcagaagcg tctgtacttg
     4861 tcggaagata agaatataca gataaagaag actgccagca aacagagaga ggtatatgag
     4921 ctgcttctaa agtgcccggg tatatcccct gcaaagctta aggaagcttg cggggattgt
     4981 ggttcggcag tcaaggctat ggagaaaaag ggatatgtta gaattgaaga aacggatgaa
     5041 tacaggcggg tctgctccaa aacatatgct gaaaaaagac atgaactgac ccaggatcag
     5101 aataaagccc tgtcagttat caggaactgc ttttccaacg gaaaggatgt tttactcttt
     5161 ggggtaaccg gcagcggcaa gactgaaatt tatcttgaac tgattgaaga gtatatatca
     5221 aagggcaaag actctataat gcttgtaccg gaaatttcct tgactcctca aatggtatca
     5281 agattcaaga accgtttcgg tgacaatatt gctgttatgc acagcagcct ttcagaaggg
     5341 gaaaaatatg acgaatggag aaaaatcagg gacggtattg taaaagttgc tattggtgca
     5401 agatcggcag tttttgctcc ctttaaaaat ttggggatta tcataattga tgaagagcat
     5461 gaacacacat acaaatcggg gataaagccc aagtatttaa cccgggaggt agcagagaaa
     5521 agatgcaggc ttgaaggtgc aaaccttctt ttgggttcgg ccaccccttc cattgagacc
     5581 tattacagag cacagaccgg agaattgggc atggctgaat tggggtcaag ggcaaacggc
     5641 agtcctatgc ctgaggttga ggtagtggat atgagggagg agcttgagtc cggcaacagg
     5701 acaatattca gcaggagcct gactgatgcc atattggata accttaagaa caaggaacaa
     5761 atgatattat ttctgaacag gagaggccac tccacctttg tatcctgcag acagtgcgga
     5821 atagtgatga aatgtccgag atgcagtatt tcactgacct atcatatgaa aaacgaaaag
     5881 ctggtttgcc attactgcgg atatgaaagg gataatcccg ccatatgccc gaaatgcaag
     5941 agcaaaaata taaaatactt tggtgtgggt actcagaagc tggagaagga gtttcagagg
     6001 aagttttcag caaaggatgt gctgagaatg gatgcggata caaccacgaa gaaaaattcc
     6061 caccaggaga ttttggacaa attcaaaaga aaggaagcaa atgtactgtt gggaacgcag
     6121 atgatctcaa agggcctgga ctttcctgat gttacattgg ttggagtgat tgcggcagat
     6181 acaagcctta atctgcctga cttcaggtcc gcagaacgaa cttttcagat aatagcgcag
     6241 gtatcgggac gctcgggaag aggcaataaa acggggcgtg taattgtcca gacatacagc
     6301 cccgaacatt acagcataga atatgcacga tgccatgatt acaaaggctt ttataataaa
     6361 gaagttatgc ttcgcaagga actcggatac ccgccctttt ccagtattgc aaatataatg
     6421 atcagcggca ctgcggagga agaggttatt gcatatgcta atgaaataag caaatttctg
     6481 gatgaagcac ttaaaggata taaggatgcg gaaaagctgg ggcctgcagc agcggcaata
     6541 tcacgaataa aaaacaggtt caggtggcag atattggtca agagcgagag ggaggaaaca
     6601 ctgagaaaag tgcttgcaga cctttcggaa gcctgtggtg aaggcaggtc aaatgtgagt
     6661 atcagtatag atttgaatcc tcaaagtatg ttatagtaat aatagaagaa taatgcgtcg
     6721 taataagttt gtgaagtact ccgactgtag gggaagtatg ttctcctcac tttccaaagc
     6781 gggggatgca agggggcgaa gcaccctgcc gttcaaggaa ggtgctcagc tgcggagcag
     6841 cgtcgaaggg aaccataggg ttccctaggg tcggaaaagc gtcgcgagac aggcttttcc
     6901 tcctggggta ttcccgttcc gaggtcgggt ggaaagtttc gtcaaagata tcttccccgc
     6961 gtcggtaatt agctttctcc tcgctttaaa gctctgacct aatctatcaa ctagtgacta
     7021 gtgactagtg actagtgact gaagaaaatt gctgcgagga tatgtaatag gattagattt
     7081 tttaggagga taatgatata aatggcaatt agaacaatca ggaaagacgg agatgaaatc
     7141 ttacgcaaga tatcaagaaa ggtagaggtt atagatgaca ggatcctgac cctccttgac
     7201 gatatgaagg atactatgaa aaaagctgaa ggagtggggc ttgccgctcc tcaggtcgga
     7261 gtattgagga gaattgtggt tatagatgta ggcgaagggc ttattgaact gataaatccc
     7321 gttattgtgt atgagagcgg ggagcagttt gaaatggaag gctgcctcag tatacccgga
     7381 ataaagggaa aagtaaaaag gcctgaaaag gtcattgtaa gggctcagaa cagaaaaggc
     7441 aagacttttg aaatgacagg aacagaactc ttggcaattg ctttttgcca tgaaattgat
     7501 cacctaaacg gtattctttt tactgataaa gcaataataa ccgataagga agattaggct
     7561 tcgaaaggga agaagaagta tgagaatagt ttttatggga acgccggact ttgcagttcc
     7621 ctgtctcaga gagctgattg ctcataagca tgaaatagcc gctgttgtga ctcagcctga
     7681 caggccgaaa ggaaggggca acaagctggc ggaatctgcg gtaaaaatat ttgccagaga
     7741 atcaggaata tcaattttcc agccagagaa aataaaaacc ggggaatttg ttcagatact
     7801 gagggaactg gcgccggaag ccattattgt agttgccttc ggacagatac tgtctcagga
     7861 aatattggat attccacctc ttggatgcat taatgtacac gcatcccttc ttccgaaata
     7921 cagaggagca gcacccatta actggtgcat aataaacgga gacaaaacca ccggtgtcac
     7981 tgccatgtat atggacaaag ggatggacac aggtgatatc atattaaaaa aagagattga
     8041 aataggagaa gatgagaatg caggggagct gcatgacaga ctgtcggagc ttggagcttc
     8101 agctttgtcc gagacgatgg agcttctgat tgaaggtaaa atcgagagga ctccccagaa
     8161 taacgaggaa gctacttatg ccccgataat gacaaaagaa cttggcagga tagactggtc
     8221 gagggatgcg aaagatatca ggaatctgat taggggaaca tatccgtggc ccggtgcttt
     8281 ttcgtcatat aacggaaaga tattcaagat attaagaagt caggctatgg acgataatac
     8341 ggacggcaaa aattacggct gtattggcag tgtacataaa gacagcatag ttgtaaactg
     8401 cggcggcgga agccttaaaa tattggatct gcagtttgaa aatgaaaaac ctatgagtgt
     8461 ggaagcatat ctaaggggac atactattat tgcgggcaca atactgggaa aatagacaca
     8521 ggataagagg ctgcgaggtt ccggagcagt ggcggacaaa gaataacgaa taataagtta
     8581 ataatattta aacaaattat ggagggaaaa atatgtttta tggttatggt ttagatccga
     8641 cttttatttt gattttaccg gcgataatac ttgccgcata tgcgcagtcc aaggtatctt
     8701 ctacctttaa taaatacctc agagtcagga acagctatgg gcatacggga tatgaagttg
     8761 ccagaaggat attggattta aacgggcttc gggatgttcc cgtggagctt gttaggggaa
     8821 ggctttccga ccattatgat ccaagaacga gagtgctcag attgtcccag gatgtataca
     8881 acagtaattc ccttgcgtct gttggggtag ctgctcatga gtgcggccat gcaatacagc
     8941 attcggaagg atatgcaccc cttgttgtaa gaaatgcaat tgcccctgtg gcttcttttg
     9001 gttcccaggc agcgtggctt tttattatag ccggatttat ttttagctgg gtgaatctta
     9061 tagacgtagg aatacttctt ttttcagcag cggtagcatt tcaggttgta actcttccgg
     9121 tggaattcaa cgcaagcagc agggcaatag cccttttgga aggcaacgga ttggtaccct
     9181 cggacgaaat agggcccgca aaagaagttc tgaatgctgc ggcacttaca tatgtagcgg
     9241 caacttttgc ggctgtcatg cagttggtgc ggcttttggt tctgagaggc agaagggatt
     9301 agaggtatat tattcgggct acagatagga ataatactat ctgtagcttt tgcgttaata
     9361 tgttatgaat agggatattg gggaatgcaa agtaccgacg tggggaaaac atacttcccc
     9421 cacagtcgga gtactttaca aacttctgca tatataaagg agtattactt atgaaaaaaa
     9481 ttgacctggc cagagatgcg gcagtacgga tagtacacag ggtgctgaaa gaaggggcct
     9541 attccaacat tgccctgaag gaggaacttg ataaagacag gcttttaagg gttgacaaag
     9601 ctcttgtcac cgaaatagta aacggcactc tgagaaatct tacccgtatt gactggataa
     9661 tatcccgttt cataaaaatg aaaagcatag acccgtggat tgaagatata ataaggtgcg
     9721 gaatatacca gatacttttt ctggacagaa tacccgactc tgcagtatgc aacgaaagtg
     9781 ccgagcttgc caggaaatat ggacatgaag gaaccgtaag gcttgtaaac ggagtactta
     9841 gaaacatcag cagaaataaa gagaagctgg aatatccgga taaaagcgag aatgccgcct
     9901 catacctgtc tgtattttat tctcatcccg aatggatagt aaaaaagtgg ctcaaggatt
     9961 tcggaatgga attcactgag gccctgctga aggcaaataa tgaaaaacct cctttcacaa
    10021 tacgctgcaa caggcttaag attgagaaaa aggggctttt ggcaatactt gcggaagagg
    10081 gcattgaata tactgacggc agctataatg cagaagcaat tcacataagg ggaacctcct
    10141 caatagaagc aaaggaatcc ttcaggaagg gttattatca ggtgcaggat gaaagctcca
    10201 tgctggtggc tcatattgtt gatccgaagc cgggggataa agtccttgac atatgcagtg
    10261 cacccggagg aaagactacc cacatggcgg agttaatgga caataaagga gagattgtgg
    10321 ccagggatat ttacaggaat aagctcaggc ttgtagagga aaactgtgac aggctcggga
    10381 taagcattgt aagaactgaa ctctttgatg caatggtaaa ggatcagggt tccatcggga
    10441 aatatgacag ggttctgctg gacgcaccct gcagcggttt cggtgtgata agaagaaaac
    10501 ccgatcttag atggaaaaag gatgcgaaga atttaaagga attggttgat atgcagcgga
    10561 ggatactgga gctggcttcc gagtatgtaa agccgggagg aaaattgata tacagtacat
    10621 gtaccataaa cagagcagaa aaccttgatg tggtaagagg ttttctgtca aataacccgc
    10681 agtttcagat ggagagtatt ataggccaag taccggaaaa gctggagaat gacagtgccc
    10741 atatgggata tcttgtgctg tatcctaacg ttcacggtac ggatggattt tttatagcta
    10801 aaatggaaaa gaggtaattg aatttgccgg ataagaagaa tattaaagac ctgggatttg
    10861 atgagcttga aatatatatt aaagagcacc ggctgcccgg attcagggcg gcgcaggttt
    10921 tcgaatggat atataaggat acggcttcct ttaaggatat gaccaatctg cccaagaacc
    10981 tgattgagca gctggaatgg gatttccata tcggcagggc atatattgcc gcaatgcagc
    11041 actcaaaaac agacaatacc agaaagtacc tgatttgctt tgaagacggt aatgccgtag
    11101 agtgtgtgct catggactac agctacggga aaaccctatg catatcaaca caatttggat
    11161 gcagaatgtc ctgcgagttc tgtgcttcaa caacgggggg gcttatcagg tccatgacca
    11221 gcggagaaat gcttgaggag gtaatggctg tatcccggga tatcggagaa agaataggca
    11281 atattgtact tatgggtacg ggggaaccct ttgacaacta tgatcaggtt atgaagttca
    11341 taaggacaat taatcacccc aaggggctga atataggcca aaggcatata accctttcaa
    11401 cctccggaat tgttccgaga atatacgatt ttgccgatga aaagcttcag tgcacactgg
    11461 cgatatcact gcacgcagct gatgatgagg ccaggagcag aataatgccc ataaacagga
    11521 aatataacat tgagaagctt atggaagcgt gcagatacta tataaatatg acaaataagc
    11581 ggattacctt tgaatatgca ttgataaaag atgtcaacga cagtgttaag gatgcccata
    11641 agttgggaac actgctaaaa ggaatgctct gccatgtgaa tcttattccg gtgaataaag
    11701 tggaggggaa aaatttttca aaagcctcca aggagaggat aaattccttt aaggccgctc
    11761 ttgagggctt cggaattgaa acaaccgtca gaagggaatt gggcagtgaa attgacgcag
    11821 cctgcgggca gctgaggcag aagcacataa atcggcaggg agtcacaaat atttgaattt
    11881 gttagaggtt ttttatatgt tttgtcgaaa tatttttttt gtaatgaaaa ccgaaggcaa
    11941 ttttaattcg gctaagagta tatttgaggt gatttaatga ggtcagccgg caggacggac
    12001 ataggtctgg tcagaaaagt aaatgaggac agatttttat gcgaaaaact tgaggacatc
    12061 gacagcacgt atttatacat tgttgccgac ggcatgggag gacataatgc aggagaggta
    12121 gcaagttcaa tggcggtaca ggaggtagct tcttacataa aaaatagtat cggaaccctt
    12181 atggcgggag atgaagaaat taaggatctt gtcagaaatg caattatgta tgccaacgaa
    12241 aagatttaca agacgtctat acttaagagc aactgccttg gtatgggaac aaccctttca
    12301 atggtgcttg caaaggacag cagcatatac ataggacatg taggcgacag caggatatac
    12361 cttatcaggg ggaaagacat atcaaggctt acggaggatc attccctggt tgcggagttg
    12421 gtaaagagag gtaccataaa accggaggat gcatctaatc atccgcagaa aaacgtcatt
    12481 acaagagccc ttggaacgga atactcaatt gaaccggatg tataccaata tgatatgcag
    12541 agcggcgatc ttatactaat ttgcactgat gggctgtcaa atgcggtgtg tgaagaggat
    12601 atagccggtg taatgaatgc tgcttctgat ttaaacgggg cttgcgagct tctgatagaa
    12661 aaagccaagg aaaagggcgg ctatgacaat ataacagccg tagctattca ggtttgtaag
    12721 ggtggtgaag ataatgatag gtagagtatt gggagataga tatgaaatat tggaaaaagt
    12781 tggcggcggc ggaatggcct tggtgtataa agcaaaatgc agactgttga atagatatgt
    12841 tgctataaag gttttgaggt cggaatttac cgaagatgag gaatttgtca agaagttcag
    12901 gagagaagcg caatcggcag ccagtctttc acatccgaat attgtcggga tttatgacgt
    12961 aggaaccgaa aacaacagct attatatagt tatggagtac atcaaggggc agactcttaa
    13021 agagcttata aagagcaaag ggaccttggg agtggaatat tccaccaata tagcaattca
    13081 gatatgttat gcattggaac aagcccataa aaacaatata gttcaccggg atataaaatc
    13141 tcacaatata ctgatacggg aagacaactc tgtgaaagtc acggattttg gaatagccag
    13201 ggccgtgtct tcaagcacta taacaaatac aggcaacgta atcggctctg tgcattattt
    13261 ctcacccgaa caggcaaggg gaggatatgt cgatgagaaa tcggatattt attcactggg
    13321 agtcgtaatg tatgaaatgc ttacgggaag gcttcctttt gaaggtgatt ctcctattgc
    13381 tgttgctttg aagcatatac aggaagagcc ggagcctccc tccgggatta actccaggat
    13441 accgaaggcc attgaagcaa taataatgaa gtgtatggaa aaggaagtat ccaaaaggta
    13501 taacagcgct tcggagataa taaatgacct cagacagtca ttggtaatgc ctaacggaga
    13561 ctttgtaaag aaaaacaggt ataccgatga aagtaccagg gtccttgagc ctataaaaat
    13621 aattgacaag gaagcagcgg ttgatattat tgaagaaccg gaaagcaagg aactgaatga
    13681 aggaaaagaa gaaaaaatcc tcattgatga ttattcggtt gatccaatcc caaaggtagc
    13741 cgagggaaag gataaacccg tacagaataa aaggaacgta aagcttacta ttgtcgctgt
    13801 ttttggggga ttactgctgg ctttgctgct gggcggagga atattgttcg ttaacagcct
    13861 ctttaccgta aaggacgtgg aggttcctga catcaggaac aatagcgaag aagaggcaag
    13921 ggaaaagcta agtgctctca gccttatact ggaagttacc gaaagggtat ttaacaagga
    13981 tattcccgaa ggaagcatta tcagccaaaa tcccaagccc aacgagaaga acaagataac
    14041 caatcccgta aaggttgttg taagcaaagg gcccaggaag gttgtagtcc cgaagcttga
    14101 aggcgaaagc tatgacaagg tcaagctcat acttgaagga gaaggactgg ttgagggttc
    14161 ctttgaccag gaatacagcg aatatcccaa tggttttgta ataaggcaga gcatagctcc
    14221 gggtatcagt gttgatgaag ggactacaat agactatgta ataagtatag ggccggaaaa
    14281 attcataatg ccctcatatc ttggagcaga tattgaagat gttaagacgg atctgattat
    14341 caaagacctt atattgggaa acatatccta tgacagcaac agtgagcagc ccgcagacac
    14401 agtgctggag cagagcataa ggccgggcac tgaggtcagc aggaaaagcg tagtggattt
    14461 tgtcgtcaac aactataagc cggagcctgt accctccagc ttcaagcttc gtttcacact
    14521 gccgccggag cttgagagta tgaaggtgac ggtatataaa gtacagaatg aaataagcag
    14581 cagaatatat gaaagtattc acaaatcaac cgacagcccc ctggaaatac cggtaagcgg
    14641 tgtagggaat gtaatttttg aagtatatat aaatgacagc ttgcacgaaa gcgtagcaca
    14701 caacttttaa cgaggaggga atgtatgcag aaaggtgtta tagtaaaagg catcggcgga
    14761 ttctactatg ttgacacagg ggaaggggta tacgagtgcc gtgccagagg caaattcagg
    14821 ttaaagaaca taacaccttt aataggggac catgttgaaa ttgatgttga tgcgggaagc
    14881 ctgcagggtt atattatcag cattctggag aggaaaaacc agctaataag gccgactgta
    14941 gccaacattg accaggctgt gatagtattt gcggcaaaaa agcctgatat aaacatggtg
    15001 cttatgcaga agttcctggt ttattcggag tataagaatc tgaatgtcat agtttgtata
    15061 aacaaaatag atttggaccc ggatggccac tatcttgatg tggtcagaat gcttgagggt
    15121 attccctata aggtaataac caccagcgcg aaaacaaaag tcgggataga gcagctgcag
    15181 gaaatactta aagataaggt ttcggttttc gcaggcccct ccggtgcggg aaagtcatcg
    15241 ctgctgaact gcattcagcc ggggctgaag ctaaagatcg gagacctgag caaaaaaata
    15301 gacagaggca cgcataccac gaggcatgca gagcttataa agcttgatga gggcggcatg
    15361 gtagcggaca ctccggggtt tacctctctt gatattggcg aactggagct gtcgaagctt
    15421 gaaagctgct ttccggaatt tgaaagccac aagaattgcc aattcgttaa ttgcatccat
    15481 gataaggaac cccgctgcgg gataagagag gcagtactcg agggcaagat acacaatata
    15541 agatatgatt tctacatatc tctgttaaat gaattgaaga gtactggggg gaggaattat
    15601 aaatgattaa gattgcgccg tctatattat ctgcggattt tgcgaatctg gagagggata
    15661 taggtacagt ggagagggca ggtgctgatt ggcttcacgt tgatgttatg gacggacatt
    15721 ttgttccgaa tataaccata ggcccggctg ttgtaaaaag cttgagggca aaaagcagcc
    15781 tggtttttga tgtgcatctt atgattgaca atcctgagtt atacataaat gattttgcgg
    15841 aagccggtgc cgatatcatt acagtacatg cagaggcttc ggtacatttg cacaggttgc
    15901 tgcaaatgat aaaggcaaag ggcctgaaag cgggtatggc attgaatcct gccactcctg
    15961 tcaatgtact ggagcatatt atagaggatt tggatatggt attgattatg agtgtaaacc
    16021 ccggctttgg aggacaaaga tttattaatt ccgcttatga aaagatatca aaggtaagaa
    16081 gcctcattga gaaaaagaat ctgtccgttg atttgcaggt tgacggagga atagggcctg
    16141 acaatatccg gcaggttgtc atgagcgggg ctaatataat tgttgcaggt tcggcaattt
    16201 ttaactcggg aaacataccg gaaacaataa gaattatgaa ggaagcaggc ataagtaaat
    16261 aatgaaaact gtaataatca gcggcggtga aataaaggat tatgaattct gcagcggtat
    16321 tgtcaggtct gcagacaggg ttatctgtgc ggacagcggt acaaggcacg caatgaatat
    16381 gaatctgact cctgatgtaa taataggaga catggattca tcaccggccc agtatataga
    16441 ttttttcagg aaaaaaggcg ttgaagtcat acagtacccc aaggagaagg ataagaccga
    16501 tacacacata tgtgtggaat acgcactgga cttctcgacg gaaataatat tgctgggagc
    16561 gacagggagc cggattgacc atatgattgc aaatatctct ctgttgatgc ttggaatccg
    16621 gcggaacata cccatgagca taatagacaa caagaattgt atcaggatga ttggggattc
    16681 cattacactg aaggggaata aaggtgagtt tttctccctt ataccctata cagggaaagt
    16741 agaaggactg agtaccaggg gagcccatta cgagcttgag aatgcagtta tggagctggg
    16801 gaacccgtat ggcgtcagca attattttga ggaagaaacc gtaagtataa ccattaagaa
    16861 aggctttctg ctggtaatta agtcggagga ttagaaaccc gagagaaaaa aatcagcttg
    16921 tcggcttgaa aaatccgtgt caaatatgac tctgcattag tattatataa tgtagggttt
    16981 ttatcttttt ttgccattat tccgtcaggg ggattcaaca atgaggtata catataagag
    17041 atatgccggc tgcttgtgta tcatactgtc tgtctttctt gtttcaagat tcatgccgct
    17101 ttttctatgg tgttttgcag tggcggcagc tgtcgcaatg ataggatttc tgctgtacaa
    17161 ttgctttttt atgtagttag gaggaatgac atcatgagtt tattaaaacg aaaaaaactg
    17221 actggcttcc ttgcattctg catagggctg ggaatactct ttgccgtcct ggttcctgca
    17281 ataggctggg taatgttctc ggcaatctgc cttattagca tcggaatatt ccttataaaa
    17341 tattgagaga tgttatttca aaaaagacaa aaagcgctga atcgcgcttt tattttcgat
    17401 tttagccttg ttatattgcc ctctgtactt tacccgatct taaacatctg gtgcatacat
    17461 gcaatctctg tggagtaccg tttactacgg cttttacagt tctgacgtta ggcagccaga
    17521 tcctgcggct atgtctgttt gagtggctta ccttatttcc ggaaatcatg ccttttccgc
    17581 aaacctcgca atgtctcgcc atggaataca cctccttata aaaacaaata tattttaaca
    17641 caacccgtta taaatggcaa caatatgttt ttaagggctg caaggctgaa gaatggcggt
    17701 gttgacgcaa tcctataatg tactttactt atacgggcaa attatagtat catttattat
    17761 gtcacaattt gatagtatta tttgtatcat ataacatact tattatgagg cgaaagtaag
    17821 aaaatttttc taaattcgaa aaatttactg ctgatgcgtt ataatatata ataataataa
    17881 aaagcgggag gagatcacat gtctgccata tacaaaactc agtacggtga agttaatata
    17941 acagatgatg ttttggcaac catttcagga cttgcagcta ctgaatgcta cggtttggta
    18001 ggaatggcaa gcaagaatgc taaagacggc ctggttgagc ttcttaagag agaaaacctt
    18061 gctaaaggtg taaaggttac aacagaaggt gaagagataa tagtggattt atatattgtt
    18121 gtcgaatttg gtacaaagat atcaactatt gcagataata ttatcagtaa ggtaaaatat
    18181 actgtagaaa acctgacagg cttgaaagta aaaaaagtaa atataaatgt acagggcgtc
    18241 agagtataac tctggaaagg aggtacatat cttgtcaaca aaaaatattg atggtataca
    18301 gttaagaaac atgtttttat ccggcgcaaa taatctggaa aacaataaag gaatcgtaaa
    18361 ctctctgaat gtatttcctg tacctgacgg cgacaccgga acaaatatgt cgcttacaat
    18421 gcatctggct gtgaaggaaa tcatagctgt caagagtatt gaaatacaaa gcgtggccaa
    18481 tgccgctgcc aatggttccc ttatgggagc aaggggcaat tcgggagtta tactttcaca
    18541 aatatggaga ggctttgcca aggcattgaa gaatcaaaaa agtatggatg taaatttatt
    18601 ggccaatgca cttacggaag gctccaatac tgcctacaaa gcaataatga agcctactga
    18661 agggactatt cttacagtca ttcgcgaaac agcagagtat gcaatgaagg tttcagagaa
    18721 ctttgaggat aatgtactat tttgggaaga aataatagag aaggcaaaca gtgtattgcg
    18781 caggacccct gaaatgcttc aggtgcttaa gaacgccgga gttgttgatg caggcggaaa
    18841 aggacttata tatatatttg aaggaatgta tcagtacctg aagacgggtg aaatgataga
    18901 actgaaagct gcagcagatg atgaagagca tgacaggccg ctgtttaaag aagaggaaat
    18961 tgtgttcggc tattgtaccg aattttttat aaaaggaaga aaactcgggc tggaaaagtt
    19021 cagagacgaa atattgccga ttggagactc acttcttgtt gttggggatg agaatctttt
    19081 gaaggttcat atacatacca acaaccctgg cagggtgctg gaactggcga tggcccaagg
    19141 tgagctttcc aggataaaga tagataatat gagggaacag cacaatgaac tgctgtttaa
    19201 taaggaggag cacaaagctg cggaagaagc ggaacaagct ccaaaaccta tggagaggta
    19261 tggaaccata gctgttgcaa tgggagaagg catatcttcc atattcaagg acttgggggc
    19321 aaacgaaata atagaaggcg ggcagacaat gaatccaagt actgaggata tattaaacag
    19381 tgtgaataaa atcaatgctg aaacgatata tatattccca aataacagca atatcattct
    19441 tgccgcaaat caggcaaagt caatgtctga taaaaatgtt atagttgcgg cgacaaaatc
    19501 catccctcag gggatatccg cacttatggc gcttaatcat gataaggaaa ttgaggaaaa
    19561 caataaaaag attgaaaagg caattacaaa cgtaaagtca ggtttggtta cctatgcagt
    19621 gagaaattcc aactatgacg gaataaatat agaagagggt aatgtacttg gcatggttga
    19681 agggaaaatc agcggggtag gcaatgatat tcttgaagtc agccataagg ttcttgatga
    19741 tatggtagat gaagactctt ctttgatatc catctactat ggcagcgaag tgaatgaaga
    19801 tgaagccctg aagctgaagg aagaaatcgg cgaactgtac aataactgcg aaatagaact
    19861 gcacttcggc ggacagcctc tgtattatta tatactctca gtagaataaa ttattgaggt
    19921 gtacaaatga attatactga agattccata accaatttaa agggaatagg accggtaaag
    19981 gccaggcttt ttaataagct tggcctgtac actatcggtg atttgctgga gtactttccg
    20041 aggggttata aagataaaag cgagattaca aaaatcaggg atataatgcc cgatgaaagc
    20101 tgtaccgtaa gggtgcgtgc cgcaggaaag gcccaggagg gcagaaccag gagcaggctt
    20161 attattacaa agctgacggt atttgatgat acaggcaggt tgaatgcagt gtggttcaac
    20221 aacagattca ttaaaaatgc tttcagggaa ggggacgaag tactgatgca cggcaaggta
    20281 aagcgcatcg ggaaggatct ggtcatggag gtccccgagt atgagaggct gtcagacagt
    20341 gaaagcataa acctgatgag aataacgccg tattattccc ttactgaagg cctgtcacaa
    20401 aaggatataa gaaaggcggt atacgcagca ttaaaacatg ctgaaaagca ggcggatttg
    20461 tttccggaag aactgcggag aaaatacgga cttgccgaga ttaatttctg catcagcaat
    20521 attcattatc cggagtctat ggataagctg aagctggcaa ggaaaaggct gatatttgaa
    20581 gagtttttta tgctgcagtc cgggttgata catataaaaa agctgaacag cgccgggcaa
    20641 aagggtatca ggtttaaaaa gacaagtgaa ataaaagagt ttattgaaca tttgcctttc
    20701 aagcttactt cagcacagga aagggttttt gaggaagtca gcaaagacat ggaaggttcg
    20761 ggcattatga acaggcttgt acagggggat gtgggttcag gcaagacggt cattgccgct
    20821 cttgcactgt tcaaatgtgt aagaagcgga tatcagggca caatgatagt acctacggaa
    20881 atactcgcag aacagcatat ggagacttta acaaacctgc tcaaaaacac aggtgttgaa
    20941 attgcactgc tgaagggcgg aatgacaggg aaggaaaaac aaaaagtgct ggagggaata
    21001 aaagaaggaa aaatagacgt ggttgtggga acccatgcaa tcattcaaga cagtgtaagc
    21061 tttgataagc ttggacttgt tattaccgat gaacagcaca ggttcggcgt aagacagaga
    21121 gcattgcttt cggcaaaagg ggaaaatccg gatatgctgg ttatgactgc aactcccata
    21181 ccaagaacac tttcacttat agtatacggg gatcttgatg tgtcaataat agatgaaatg
    21241 cctcccggtc gaaaaaaagt agagacttat ttcatagatt ccgccaaaaa ggaaaggctt
    21301 atgaattttg tcaaaaaggg ccttgatgaa gggcggcagg catattttgt atgcccgctt
    21361 gttgaagagt ccgacaagct ggagcttaaa tcagcgatag agtacagtga ggaactaaaa
    21421 aataaatact tccggaacta taatgtggga ttgcttcacg gaagaatgaa agccgatgag
    21481 aagaatgaag taatgtacag gttcagggaa aaagaaattc atatacttgt atcaacaact
    21541 gtaattgaag taggagtaaa tatccccaac gccgcctaca tggtaataga agacgctgac
    21601 agatacggaa tggcccagtt gcaccagttg aggggcagag tcggaagagg cggacaccaa
    21661 tcctattgca tattaatttc tgatacaggg aatgaaatag cgcaggaaag gcttaagtat
    21721 atgacaaaaa cggaaaatgg ctttgaggta gccgagaagg acatggagtt ccgcggaagc
    21781 ggggaggtac ttgggcaaag acagcacggg cttcccggct tcaagcttgc tgatgttttt
    21841 cgcgatgcga agcttcttaa gctgtcaaag gaagcagtag aatacatata tgaaaataat
    21901 aagctttcca atgaggatta caagaagatg aaagaaatac tggatcaaag gttccagaag
    21961 atgctggatg aaataacgct taattaggcc tcgaatattt tcctaagtta acataattta
    22021 cagtgtttaa aaaactgtaa acggagtaaa ttaataatac ataaaccgca ccaagcattt
    22081 acgctgcatc ggggctgcat tcgacgggac tattgcggtt tatacacgta atacaaacag
    22141 gaggtgctac atatgccaag aaataccaca tcaaacagga ccttgatacc ggaagcaaag
    22201 gctgctttga agactttcag ggatcagata gctactgaat taggaacgga tcttacaggc
    22261 tataatggtg ataagacatc ctgggagtgc ggaaaagtag gcggagaaat ggtaagaaga
    22321 atgattgaac aggcagaaag aagcatgtca ggaagaagat aaaagataaa ggtaaaagaa
    22381 aaagaaaggc gctggctgca taggtcagcg tcattttttt agcgaaaaaa tactgatgac
    22441 aacactctaa ttcaatgtta aagcattgac atcagtatcg ggagaggaga aattgaagga
    22501 agagctctat ggggaaattt agttacaaat aactaacgtc cctgatatat atgttgttca
    22561 aataagtttt ttttattcaa ttaaaattat tttacatacc ttcctttaaa tacatttata
    22621 aaaggccccc atttttatca gactgttaat gacagctttc tcatatcgca gaatctgtcg
    22681 attccctgac atttatgata taatatgcgc aggtgcatat tatattcctt atataattgc
    22741 cgcgcgatat taataaattt ttgtataaaa atagtattta tgttaaaatg taattgaaat
    22801 gggcaaaatc aatcggtgtt attgacaata aaagacttgg aggtaatctt ttgcgggtta
    22861 ttgcaggaga gtataaggga agacggcttg atcccattga gggcagggac atccgcccta
    22921 cctctgatat ggttaaggaa tctcttttta atatacttgg ggatgctgta attgacagcg
    22981 tttttctgga tttgttcagc ggtaccggag gaataggcat tgaagcattg agccgcgggg
    23041 caaagcatgt ggtattcatt gattccggca tcaaaagcat aaaggtactg aggggaaacc
    23101 tgaaccgtct gaatatcaag gattgtgctg aagtatataa tacggactac gggacggcaa
    23161 tcaacaaatt gcacgaacat ggaaagagtt ttgatttaat ttttattgat cctccttaca
    23221 gtatgggaat ggctgaatca gccttgaagg aaattgataa taaaaatatt cttacacaat
    23281 ccggtttgat tgtagttgag catgattcaa aggaggatat gccgcaaaaa gtggggaagc
    23341 tattcttata ccgtttcaag aaatatggaa atacaatact atcattttat tctattgacg
    23401 aacagtcgca gcaaaaggag gagtaatttt gaaaatagca gtgtatcccg gaagctttga
    23461 tcctgctact aacggacata ttgatattat taaaagatcc ggagcacttt ttgacagggt
    23521 tattgtagca gtattgagca atccgagtaa gaatccgatt ttttctgttg aggaacgagt
    23581 ggacatacta aaaaggacat gcggagatat aaagaatgtt gaaattgaca gtttctcggg
    23641 gcttttgatt gattattgca agagtaaaaa cgcaaatgta atagttaaag ggttaagggc
    23701 agtttcagac tttgaatatg agctgcaaat ggctcatatg aataagaagc tcaatcagga
    23761 aatagaaacg gtgttcataa tgactagcag ccgatattca tatctgagtt ccagcctggt
    23821 aaaggaagtt gcaggatttg gaggctgcat aagagggctg gtaccggaaa ttattgagaa
    23881 ggagatttat gaaaggctta aatagcggtt caggcacgga ctatcatacg tgtgaaataa
    23941 atattcacaa atgaattatt acagggaggt tggtattatg gaggttatgc tgctgctcga
    24001 cgcccttgaa gatatactgg ataaggcgcc gaatctgccc ttatcaagta agtccatgat
    24061 taataaggaa gaattgcagg aaataataaa ggaaataaga ataaagctgc ccgatgagat
    24121 aaaacaggct caatggatta aggaagaaag gcagaaaata cttatagaag caaaaaaaga
    24181 agctgaaaat ctgagaaaag aatgcgaaga gaaactgaac aaggttaagg aagaacgtca
    24241 gaggattctt gccgaagctg aaaaagaagc tgaaatactt aagaaggaag ctgacaaaaa
    24301 ggttcaggag cttattgatg aaagtgatat aataaaaaag gcaaatgaac aggcaaaaga
    24361 acttattatt gctgctcaga tagatgccaa aaagataagg ctgggttcca aagcatacgc
    24421 ggacgagctg ctggcggaac ttgaaaaaca cacggaaaaa ataatagaaa caataaggaa
    24481 caacagggat gagcttaaga attacaagag ctgataagtg gtacaagggg gacgtttctc
    24541 ttgtaccctt ttaatttatt catttgggac aggagaaacg tccccatgta cctttaatat
    24601 gcaccgcgga cataaaaagc aatattatca gcatatttgc ggcagaggta accaataccc
    24661 gcaaaaagct gtattccagg atcgtatttt gcacaaatga ttctgccgga aggttaaaaa
    24721 cctgaagatc agaaaaccag gcaaccagta cataggagta gaatgcagcc gcaattccct
    24781 gaaaaagctt ggtaatgaaa taaataccga gcttaagatc cgttccggat attatactca
    24841 tcacctgggc atttactgaa agccctccaa atcctatcat gaaactcgtt atggcagcct
    24901 tgtatattaa gggcacccgg agagaggcac atgacttcgc cccgttggtt acttccagta
    24961 agccgataag cagactggat ataatatcgg gagttatgct cttaaggggg attgcccttg
    25021 aaaggctgag ggagaaccat tcgagaaagc ctgatattct cagcatgccg gttatcacag
    25081 aaaagaagat aataaaaccg cctatcataa gtatcaggtc gattccgttc tttaccgcat
    25141 ctctcatcag cattccgaaa gtacgtccgt ctctccttct gtactccatc atttcagcaa
    25201 taggatttga tatatatttt tccctgatgc ggggattttc tttcccataa aaacgcattg
    25261 caaacccgat tgtgatggct gacaggtagt gggcaattgc aataaaggag cctaaggccg
    25321 gattgttcaa tattccggtg gcaaccgcac ctattatgaa caaaggcccc gaggtgctgc
    25381 acaagctcag cagcctctgc ccttcagcaa gagtacacag gtttttgctg tacagttctg
    25441 cggtaatttt tgcgcctaca ggatacccgg aggatatact cataaaaaaa tcaaaggagc
    25501 cttccccggg cacattgaat accggaccca taaggggacg gaaggcggaa ccgaacagtc
    25561 tgacaatacc caggccaatt aagatttcaa cacagataaa gaagggcaga agtgcggggc
    25621 agaccacatt gaaccatagc ttcaaaccat caagtgaagc tgcaaaagcc tctcccggat
    25681 atctgattat gaatgtcaca aggatacaga agataaagga ataagcaaag gtggaaatag
    25741 tgcctttttt cataaggaac ctctaagcat aagtatttta attaatatat ttatatttag
    25801 agggatatga tattatgaat gaacagagag tattactccc tgtgataata gggagaggta
    25861 aaataatcct ccgcagcttt cctgaattca gatgtcctgt atgccaggtt gtaaatgtct
    25921 gtagcctgta tatcatatga aaacattttt attattcttt tatcggtgca tctgaaatcc
    25981 gccacctttg aaatcacagg gtaagggcag cagtccatta tcattttgat aagttttttt
    26041 ccattattgt tgaaagccag tactctaaca taagacgggc attcccggct tttgaacatg
    26101 gatatgtctg cattcttgat atcaaggagc acgtgacaca gcaggcgctg gatgtaggtt
    26161 cttgtgtaac gttttgtttt gacagcttca atcagatctt cgcaattggt gctggctgct
    26221 gcatatttct taagcctgtt ttctattccc tccttaacat tagggtattt caaaagctcg
    26281 gatttttcca ttgtcctaag cttgtacagc agtatatccg aaaaatcatt catataaaca
    26341 ggatgcctgt tcatcctcag atttacactt ataatatcta tggagcttcc gggcagggac
    26401 attcgaacct tgtcggagat tccgccttct tttatttcat ttcttattgc agtggcactt
    26461 gcgatgcttc ctgcaattgt attatcatta taaccgctta cgatacgttt tacggcaaca
    26521 ggtgtaatag agctattcaa agacttgaga gctttaatat attcaatacc cagtacatta
    26581 ttgggattct tcaaaatatc atcggaagca taatgaatat catcggatgc ataatgggac
    26641 aatgcgttgg aaacagcttt gggataagaa acaccccggc cgagctgccc ttttaccaga
    26701 tgtttaaagg cttccggctc attttgaagc aaattggcta accggtgcag tgcgccggta
    26761 tcccccagtt cgcttccaaa acagatatgg tcaacaaccc ccagggaatc taatatcttt
    26821 accgctccgg ccgcaaatat ttccgccggc tggcaggcat acacaacagg aagctcgatt
    26881 accaggtcag ctccgttcat aactgccgat tttgaccggg accatttatc gaatattgac
    26941 ggttcgcccc tttgcacaaa gcttccgctc ataatgcaga cggcataatc tgcaccgcag
    27001 gcttcttttg cggctcggat atggtatttg tgaccgttat gaaaaggatt atactcggat
    27061 actattccta cggttttcat cattattacc tcccaaacct aaccttcgga acctcaaatt
    27121 cattttacca tattatccgc aggcaaatga atatttatag taggtattga aaaaatctca
    27181 ttatggggat aataatcttt gttaatattt ttacaaaaaa gcagggaaaa cgacgattag
    27241 tatagaaatt cttacatgca caatatataa tgggaggtac gtaaacgaat gaaaattttg
    27301 gttattaact gcggcagctc gtcattgaag tatcaattca tagatatgga gaatgaagaa
    27361 gttatcgcca aaggtcttgc ggaaagaatc ggaattgacg gctcagtact taatcatcag
    27421 ccaaagggct tcgacaaaat aagaatagaa aggcccatgc cgacgcacaa agaggcattg
    27481 cagacagtgg ttgatgcatt gagagacaaa gaatacggcg taataaagga tatgggagag
    27541 ataactgcag ttggacatag agtcgtgcac gcaggtgaga agtttgcata ttcagtgctt
    27601 cttaaagaag aagttatgga tgcactgaag gaatgcataa gtttagctcc tttgcataac
    27661 ccgcctaata taatgggtat agaggcatgc aagcagatta tgccggaagt gcctatggtt
    27721 ggagtgtttg atacggcctt ccatcagact atgccaaaaa agtcatacat ttacccaatt
    27781 ccctatgaat attatgaaaa gtacaggata agaagatacg gcttccacgg cacatcccac
    27841 aaatatgtgg ctgagagggc agcggcaatg cttggaaagc ctgttgaaga gcttaagatt
    27901 attacatgtc atttaggaaa cggtgcaagc attacagcag tgaagaacgg aatatcggtg
    27961 gataccagca tgggctttac acctcttgaa gggcttgcaa tgggaacaag atcaggcaac
    28021 atagatgctg caataattaa attccttatg gaaaaagaaa atatcagcgt tgccaaggct
    28081 gatgaaatac taaataaaaa atccggcgtt ttgggcatat cgggagtcag cagtgatttc
    28141 agggatattg aagaagccgc caataaaggc aatgaaagag cgcagcttgc ccttgacgta
    28201 tatgttcatt atgtaaagaa atatataggc gcatatgcgg ccgtaatgaa cggtgttgat
    28261 gcaatcgtat tcacagcggg cctcggggaa aactccgcat caatgagaag cagtatatgc
    28321 aaagacatgt cattccttgg cgttgagctg gatgaggcga aaaacaatgt cagaggaaaa
    28381 gaaactgatg tgtccaagga tggctcaaag gtgagagttc tgcttattcc taccaatgaa
    28441 gaactcatga ttgcaaggga tacagcggag cttgcaggga aggcaaaaaa ataaaaaata
    28501 atgcggcgcg agccgcttat ttttttagaa tgcattgtaa atactaacac aagcggtgtg
    28561 aagtgttttt gcttgacaaa atatatattg atttgtataa taaaatttgt taatcagaga
    28621 gaaaataaca ggtggttgaa tggaacttaa ttttgcaggg atatctaaat ccgaaggtgt
    28681 aaaagagaga ttcgaaataa tagaatatcc tattgacagt actattgtgt tctgcggtga
    28741 gaatttgaat atagtttctc ccattacggt gaaaggcagt gcggtcaact atgaaggaaa
    28801 gataagtgtt gatcttacga ttaccgcaac agtggagagg acatgttcca gatgtttgga
    28861 aatcttcact gaagaagttc aggcagattc aaattatgtt tttgtcaaag atatagcgga
    28921 tgacaaggaa gactattata tatacagagg agacaaggcg gacataacag accttgttat
    28981 tggagatata gcagccaagc ttgccatgaa gccgctttgc agggccaact gcaaaggact
    29041 gtgtactgta tgcggaaaga ataagaacag tatcgactgc caatgcaaaa gcgaagaagt
    29101 tgatccaagg atgcaagctt taagcaagct gctggacaga aagtaaggag gtgtttatat
    29161 tgggtttacc gaagagaaga cactcacaat cgagaagaga taaaaggaga gctaattgga
    29221 aattggcttt gccgggactg gttgcatgtc cgcagtgcca tgagccgaag cttcctcaca
    29281 gggtatgcaa atcttgcggt tattataaga acagaacggt tataaagaca gaagaataat
    29341 aaaaaattca tgataagaca agggaagtcc cctgtcttat ttattttttg cagacacggg
    29401 gaggagacac gggggaggac acggggaggg agacacgggg acgtttcgcg gagacacggg
    29461 gacgtttccc ctgtcccact ggaagtggga cacgcgaaac gtccccgtgt ctccctccgt
    29521 gtctccctcc tccgtgtctc cccgtatttt ttaaaaaaaa actgttgcat atttaatgtc
    29581 aagtgttata tattagtatt agaaccaggt actaaattat agtagtatat ctgagtattg
    29641 gaggtttgaa tatgaagcag gggggcatta gcaagaaaga aagacaggcg agtttgctgg
    29701 acagaatcaa gacagaccct tttcttactg atgaagaact tgcggaaagc ttcaatgtga
    29761 gcatacagac tataaggctt gacaggcttg agctgggtgt accggagttg agagaaagaa
    29821 taaaaagtgt tgcagaggat aactatacaa aggtcagatc tcttcatggc aaggaaatat
    29881 tcggagagct ggttgatttg aatcttggag taagcggaat atctatactt gaaactgata
    29941 ataaccttgc atttgaaaag acaaatgtag tgaaagggca gtacatattt gcacaggccg
    30001 aatctcttgc cattgcggta attgatgcaa atgtcgctct tataggagtt gctaatatca
    30061 agtacaagaa tcccgtattt gcgggtgata agctgatagc aaaggcagaa gtcgtaagaa
    30121 ccagaggcaa caagtatttt gtgtgggttt ttataagagt aaagcaggct gaagtattca
    30181 gaggaaaatt catccttgtg tccttcagca attagactaa gaagcggcta tatatgtcgt
    30241 gaaaatgatt tatcattaca aaaccagtta aagtaatttt cacgacatat gaattaagga
    30301 aaatgtttca cactatttga attcaataca aaatgtttag tgcgaaacat ataggaggtt
    30361 tgaatatgag gatagctgtt gatgtaatgg gaggcgacaa tgctcctgga gcaattatcg
    30421 aaggatctat tctggcactg aaagaatacg gaacagagct ttatttaatc ggtttaaggg
    30481 acgagatatt gaaatacctt ggagaggaat acaaaaatca ccaaaaggtt aatatcatcg
    30541 aagcctcaga aataatcaac gcagatgaag cacctgtaag tgccattaag cagaaaaagg
    30601 attcttccat ggtaattggc cttaagcttg tgaaggaagg acagtgcgat gcttttgtat
    30661 ctgcaggaag taccggagca tttcttgcag gctccctcct taaggtggga aggataaaag
    30721 gaatagacag gccggcactt tcaccgctgc tgccgacatt aaaggggagc tgcatgatta
    30781 ttgatgcggg ggcaaatgcg gacagtaagc cgaaaaatct tcagcaatat gctgtaatgg
    30841 gatccatata catggaaaag gtaatgggga ttaaaaaacc aaaagtcgga cttttgaata
    30901 ttggtgccga agcatccaaa gggaatgaac tgaccaagca gagctatact ctgataaaaa
    30961 atacaggggt aaatttcatc gggaatactg aagccaggga tattatcagc ggagtctgcg
    31021 atgtatgcgt atgcgacggt ttcgccggca atgtgctttt gaagaccaca gaaggtgtag
    31081 ctatgacatt atttaagctt cttaagaatg agtttatgaa aagcttgaaa acaaagcttg
    31141 gggctatgct gcttatggac agcttgaaaa atataaagaa aagctttgat tatactgaag
    31201 taggaggagc accatttctg ggtattaacg gcattatgat aaaagcccat ggcagttcgg
    31261 atgcaaaagc aataaaaaat gcaataagac aggcaaaaat gctatatgat aacaaatgcc
    31321 tggataccat aatggaagaa attatcaagt tgggagttga agacactgat gaacaagctt
    31381 agaaatgcag gaataatagg aacaggaagc tgcctgccgg ataaaatagt gacaaaccag
    31441 gatatggaga aaactgttga taccacagac gaatggataa gaacgcgtac aggaataagt
    31501 gaacgaagaa tgacggatga aaatactgcc acctctgatc ttgcaactat tgcggcaatg
    31561 agggctctgg caagtgcaaa tctcagtcct gaagaaatcg atttaattat agtttctaca
    31621 gttactcccg atatgatgtt tccttctaca gcctgcatag tgcagaagaa tattggagca
    31681 gtcaatgccg ctgcttttga tcttgaagcg gcttgtgcag ggttcttata cgggctttcc
    31741 attgcagaca gctttgtaag agcagggagc tttaacaaaa tacttgtaat cggtgcagag
    31801 actctgacaa aaatacttga ttttactgac agaaatacct gtgttatatt cggtgacggt
    31861 gcaggagcag caatagtatc ggaggtacct gagggatatg gaataatgtc tacatacatt
    31921 ggggcggacg gaagaggagg agatcttctg aaagtacctg ccggcgggtc aaggattcct
    31981 tcaagtgttg agagcgttca aaacagattg cattatgtga aaatggatgg caaagaggta
    32041 tttaaatttg cggtaaagat catgggggat gctgctgaga ttgcattggg aaaatgcggg
    32101 cttacaaagg aggatgttga cttttttata ccacatcagg cgaacataag gattattgaa
    32161 gcctcagtaa aaagattaaa aatagcccat gaaaaggtat atataaactt ggataaatac
    32221 ggcaatatgt cttctgcttc agttgcaatc gctattgacg aagcaaacag aaaaggtttg
    32281 ttgaaggatg gagatactat tgtacttgtg ggcttcggag gaggcctgac ctggggatcg
    32341 gcagtattaa aatggaaaag catcaggagg gattaatatg tttaagactt caatctgcga
    32401 aatattagat atagaatacc ccatactgca gggaggaatg gcttgggttg ctactgcaga
    32461 acttgcggca gccgtatcca atgcgggagg cctgggtata ataggcgcgg gaaatgcacc
    32521 gggaagctat gtgagagatg agataagaaa ggtgaaaaag cttaccgata aacccttcgg
    32581 agtaaatata atgcttttat ccccctttgt agatgaggtc atagatattg tttatgaaga
    32641 aaaagttcct gttattacca caggtgccgg aaacccggga aaatatattg cccggttcaa
    32701 aaatatcgga acaaaggtaa taccggtagt cccagctgta gctctggcaa aaaaaatgga
    32761 gcaggagggt gttgacgccg taatagcgga aggcacagaa tcagggggac atgtgggaga
    32821 attgactaca atggcattgg tgccccaggt aatcgatgcg gtaaaaattc ctgtcatagc
    32881 agccggagga atagctgacg gaagaggcct ggtgtcggta ctggctcttg gtgctcaagg
    32941 ggttcagatg ggtacaattt ttgtatgcgc ttccgaatgc acagcccatg aaaactacaa
    33001 aaaggcaatt ataaaggccg gagacagggg aacagttgta acaggaaaag ccacagggca
    33061 tccggtaaga attctgaaga ataagctggc acgggaattt gaaaagcttg aaaaagcctc
    33121 tgcagccgtt gaggaatatg aaaagctggg agtgggacgt cttagggctg ctgtagtaga
    33181 aggcgacgca gactatggtt cggtaatggc gggacagatt gcaggccttg taaaggaaat
    33241 aaagcctgca aaggatatta tagaagacat actgaatgag gcaaatgaag taatcagctc
    33301 aattaatgga ttggtgaagg aggaataaaa tgccgaaaat agcttgtatt tttccgggac
    33361 aaggagcaca atacccgggt atggggaagg aattggcaca aaattacaag gaagcaatga
    33421 atatatttga ccttgccagt gaaagactgg gaattgatat gaaaaagctt tgctttgagg
    33481 gtgatgaaga agagctgaag aaaaccgaaa atactcagcc ctcaatcctt actgtgagta
    33541 tagcattgct ggaagtgctg aagctcaagg gtattgaacc tcaaatagca gccgggttaa
    33601 gccttggaga atactcggcc ctggttgcat caaaggccat aagcttcgcg gatgcagtcg
    33661 cattggtgaa aaagagaggg aagtatatgc aggaggcagt tcccgcagga gaaggcacaa
    33721 tggcggcaat aatgggcatc gaaagggaat ccgtgatgga atgccttaag atggcaagcg
    33781 gttatggtgt ggtagaagcg gctaatttca actgcccggg acaggttgtc atagccgggc
    33841 atacaagtgc tgttgaaaag gcatgctcca tactgaagga aagaggagca aggaaggcaa
    33901 ttatgcttcc ggtcagcgca ccttttcatt caagcctgtt aaagcctgca ggggaaaagc
    33961 ttgcagccga acttgaaaat gtgaatatct cggataatga gcttcctgtc gtctctaatg
    34021 taaatgcaca ggtaataatg aacaaatatg aaataaaaag atgccttata gaacaggtaa
    34081 gcagttccgt gctttgggag gacagcatca gacggatgat tgatctggga gtggatacgt
    34141 ttattgaagt agggcccgga aggacactat ccgcttttgt taaaaaaatt gacaagagcc
    34201 tttatgttca taatgtggag aacatcgaaa ccatggagaa tactatagta agcataaggg
    34261 agagattgga atgcaattaa aaggcaaaac cgcagtcgtt acaggcggct caagaggaat
    34321 tgggagggcc atagcacttg aacttgcatc ctgcggagca aacgtagttg tgaactacac
    34381 caggaattca aaagcagccg atgaggttgt cgcggaaatt gaagcaatgg gttcgtcagg
    34441 gatggctgta aaggctgatg tttccatagc ttccgaggta gaaaatttgg tgaatgaagt
    34501 attaaaaact ttcggcagca tagatatatt agtaaataat gcagggatta caagggataa
    34561 cctgataatc cgcatgacgg aaaaagaatt tgatgaagtt atcaatacga acctgaaagg
    34621 tacatttata tgcacaaagg ctgtatccag agtaatgata aagcaaaagt cgggcaaaat
    34681 aataaatgtt tcctcagtgg tcggtatagt tggtaacgcg ggacagtcca actatgcggc
    34741 agctaaagcg ggactcattg gttttacaaa gtccatggca aaggagctgg caaagagagg
    34801 catcaatgtt aatgcagttg ctccgggctt tattcagacg gatatgacct ctgtacttcc
    34861 tgacaatgtg aaggaggagt ttcttaaaag tattcctctg atgcgtatag gaaagcctga
    34921 agatatagca aaaacagtat tattcctagt gtcagaatat tctgactata taaccggtca
    34981 agtaattaat attgacggtg gtatggttat gtaatattat catattataa aaaaggcttc
    35041 tattgaagta atctaacaat taaggaagcc ttttatgaaa cttgcttacg gggcatgata
    35101 aattaaggtc gaatgaatgc ccctactcgt tataaaatcc tgaagggagg tgaactggat
    35161 gatttttgaa aaagttcagg agaaagtagc tgagcagctt ggtattgaac cagaagagat
    35221 tacattggag tcttccttca ttaatgattt gggtgctgat tcacttgata tagtagagct
    35281 gttaatggcg ctggaagaag agtttgatat agaaatacct gatgaagaag cagagaagct
    35341 gtctacagtt ggtgatgtgg tggaatacat aaaaagcaat aaataaaaaa tgtcccgata
    35401 gcgggacatt ttcattgctg gatatgtaat gtattctgtt cattagtaaa tatatatatc
    35461 atcattataa ggagaggaaa cgattgaaca agagaagagt ggttgtaaca ggaataggag
    35521 ttatttctcc catcggaacc ggcagggaga aattctttaa aagcctgaaa gaaggcgtaa
    35581 gcggcgtggg tcatataaca aggtttgata cggaaggctt tgacacaaaa atagccgccg
    35641 aggtaaagga ttttgatccg tccgggtact tcgacaggaa ggaatgcagg agaatggaca
    35701 gattttcaca atatgcaatt gccgcttcca aaatggctgc ggaggattca aatattaata
    35761 ttgaatcatt ggacagcagc aggttcggtg tttgcatagg ttcgggcata ggcggaatgg
    35821 agaccatgga agcacagtat gatatattga gggaaaaggg gcccggaagg gtcagccctt
    35881 tcatgattcc catgatgata tccaatattg ctgcgggaaa catttccatt gccttgaatg
    35941 caaagggccc cagcattaca gttgtaacag catgcgcatc tgctaccaat gctatcggtg
    36001 aagccttcaa gattattcag agaggcgacg ctgaaataat ggttacagga gggactgaag
    36061 cctctataac acctttatct ttagcaggtt tttgttctat gaaagcttta tcaacgagaa
    36121 atgatgaccc aaaagcggcg tcaaggccct ttgataagga tagagacggt tttgttatgg
    36181 gggaaggagc gggaatgctg attattgagg agcttgaaca tgcactgaaa agaggcgcgg
    36241 taatatatgg ggaaatagca gggtatggcg ccaccggaga tgcatatcat attactgcac
    36301 cggctcctga cgctgacgga gcatacagag ccatgctcat ggccgtcaat gatgcgggaa
    36361 taaatgcctg cgaactggat tatataaatg cccacggaac ttcaacggac ttgaatgata
    36421 aactggagac cctggcaata aaaaggcttc tcggagaaga agcaaaaaaa gttgcagtaa
    36481 gctctacaaa gtcaatgact ggacatctcc ttggggcagc aggagcaatc gaggcaatag
    36541 catgcttgat ggcaataaat gaaggagtga ttcctccgac aatcaactat acaactcctg
    36601 atgaggagtg tgatttggac tatgttccaa ataaggctgt caacaggaag gtagactggg
    36661 ctatgtcgaa ctccttgggc tttggaggcc ataatgccag tattatattg aaaaaatatc
    36721 aataagtgat attataggga ttgggaatac tcgttggggg ttattgccaa tccctatttt
    36781 tatttcaaaa gcgcatatgc ttttgccaat aaacgaaaag gcagttggga ggaatggaaa
    36841 tgtccgttat cgatgaaaag cggctgaagc agttaaatga atttgagagt atcattggat
    36901 attccttcaa gaatatcagc atattaaaca atgcccttac tcacagctct tacgtggctt
    36961 caaggtcaga tcatcatgaa cacaatgaga ggcttgaatt tatcggagat gctattcttg
    37021 acatggtaat aagcctatat ttgtataaaa agtgcaagaa cctgacagaa ggaagcctta
    37081 ccaggttcag ggcggggatt gtgtgcgagc agtcactgta taacgcatca aacaacctga
    37141 agcttgggga atacctgctg ttaagcaaag gtgaagagaa tacgggaggc aggaagaggg
    37201 tttcaatact ggcagacgct tttgaggcag ttattgcagc tatttacctg gacggcggca
    37261 taaaaaaggc aaaggagttt attatgaaaa atctcgagac tactattaag aatgcagttg
    37321 agaataagtt attcagtgat tataagtctt tcctgcagga gtatgtccag aaaaacaatt
    37381 tgggtaagct gagttataag ctggtcagag aggaaggacc ggatcatgat aagacctttg
    37441 aagtggcatt gtatttaaac aatgggatta taggaagagg agagggccgc agcaagaagg
    37501 atgcgcagca gtctgccgca agagatgcca tagccggcct gggtgtaagg aatgtttaag
    37561 aagcattata tcattccgat ttttgtacct cataaaggct gcccccatga ttgcatattc
    37621 tgcaaccaga aaaaaatcac aggccaaatt gagaaagtga cagaaaagga cgtaaaggat
    37681 aagattgaag attatcttga tatgatacat gaaggcggca gccatattga agttgcattt
    37741 tacggcggga gttttacggc aatccctctt gaataccagg agcagctgtt ggatgcggct
    37801 tttgtatatg ttaaagccgg aaagataaca ggcataagag tttcaacaag acctgactgc
    37861 atagatgcaa ggatttcgga gaatctgagg aaatatggag tacggacagt ggagcttgga
    37921 gttcagtcaa tggacgaaga agtacttaag ctgtgcaaca ggggccattc tccgggggat
    37981 gttacaaacg ctgtcgggat tctgaaggaa ttcggctttt cagtcggtgt acagatgatg
    38041 ataggcttgc ccggagatac tgaggaaaag gctgtgaaga ccgcaaaagc ccttatcgca
    38101 cttaagcccg atatagccag aatatacccc gctctgacaa taaagaacac gtatatggaa
    38161 gaaatgttca tcaggggaga atatcaaccc ctgacccttg atgaagctgt tgaattatca
    38221 aagaagctgc tgatactttt tgagagtaac ggaataaatg taataaggat agggcttcag
    38281 cctacagaga atataaattc ggggaaggat gtcgttgccg ggccctttca tccttcaatg
    38341 aggcacctgg ttgaatccct catatacagg gacatgttgg cttatttatt ggaaaaggaa
    38401 ggagaaaaga agaaaattac cttgggaata aacccgaaaa atgtttctga attggtgggg
    38461 cataagaaaa gcaatattga ctacataaaa tcaaaatttt ttatcgacaa aattgaaata
    38521 tttcaggata ataatgtaga agccggtact atggtgctaa ataatggtaa aaattccgtt
    38581 attatgtcaa agaaatacta ttattccatt ttgtctaaat aaaggtgttt ggagatttat
    38641 atagaatata tataatcaac tatacagaca tatttaggag gtattccaaa atggaggtac
    38701 taaaagtatc agctcattca caaccaaaat cagttgccgg agctctcgcg gctgtactac
    38761 gggacagagc gacagctgaa atacaggcag ttggtgcagg tgctgtaaat caagcagtaa
    38821 aagcaatagc aatcaccaga ggttttgtag caccaaacgg tatagacctg gtagtagtcc
    38881 cggcattttc agaaattcaa attgacgggg aggatagaac tgcaataaag ttcatagtgg
    38941 agccaagata aattattggc taattttctg aaagcctata tcataacgga tattgggctt
    39001 tttgttttgc cctgtgacct atatcctata gcatataagg acaaggtatg ttaaaatata
    39061 ttggtaaaaa tctgttgttc gggagtgacc tgatttgtat ttgaaacagc ttgaggtaca
    39121 tggttttaaa tcctttgcgg aaaaaattga acttagcttc ggcaatacaa taaatgccat
    39181 tgtcggtcct aacggaagcg gcaaaagcaa tatttccgat gcaataagat gggtattggg
    39241 agagcaaagt gtaaaggcct taagaggaaa caagatggag gatgttatct ttgccggcag
    39301 cgacagtgaa aaagccctgg gatttgctga agtttcctta tgcattgaca acagcgacag
    39361 aaagataccg gtggattata cggaaataaa tataatgaga agaatgtacc ggtccggtga
    39421 aagcgaatac tttattaata agacttcatg caggcttaag gatatctatg agctgttcat
    39481 ggatacagga atcggcagag acggttactc aataatcggg cagggaagga ttgacgagat
    39541 actcagcaat aaatcggagg acaggagaaa tgtgttcgaa gaagccgcgg ggatagtaaa
    39601 atacaagaca aggaagcatg aagccgagaa aaagctggag cagacaaatc agaacatagt
    39661 aaggctgggg gacataattg aagaactgga gtcccagttg gggccgctta aggagcaggc
    39721 agaggtcgca aagcgctaca gaagcattat ggaagaattg aagtcaattg aaataaatat
    39781 tatgctgaac agcattgata aattccgcag caggctttcg aatgcgaagc aggaggcaga
    39841 gaacatacag agccttatag atgccaagaa ttcctctgcc cggcagctgg agcataagca
    39901 aaatgaactt aaaagaattc tgaatgatat agagacaagt attgacatgc taagcgagga
    39961 tattcaccac cttgaaaaca gcaaggaaaa aatggaaggg gaaagcaatg tccttcaggt
    40021 aagaataaat aatcttgagg agaacacctt aaagctcggt aaaagcggcg aagagcttga
    40081 gaatgaactt acagagtcaa ggcaaggtat tgaacagaac agactgaagc ttgccgagct
    40141 taatactgca tatattgagg tatacggtaa aatcaatgaa agaactgccg actttgaatc
    40201 caactttaag gccataaatg accttgaatc ggatttaagt gataaacagc agaaattcat
    40261 aagcctgatt aacaattatt cggagctgaa aagtaaaata aacagcacta atatattcat
    40321 agaaaatatg aataagagga aaagccaggt agagggggat gtgaacctgc ttgaactgca
    40381 gcagcaggat aaaaagaagc agtatgataa atcccttgaa gaactggatg acttcggaag
    40441 aaaaattgaa gactgcaaaa cacagataga ggagataaaa gggaagaaac aggaatgcga
    40501 gagtgaaata ctgttgctgg aggataaggc agggaagctg aaggcggaga aagaagccat
    40561 gttttcaagg cttaaggctc ttgacgatat ggaaagggat cacggcggat acagcagggc
    40621 agtaaaggtg cttatggcgg aatatgacaa ggtaacaaaa ggtttctgcg gtgtcgtagg
    40681 tgaaatgatt aaagtaccct ccaagtatgt aaccgctatc gaaattgctc tgggaggctc
    40741 cgtgcaaagc ataattacaa atacggagaa tgatgcaaag cttcttatag aataccttaa
    40801 ggagcataag acggggaggg caacctttct gcccataagc tcagtaaaac caagattttt
    40861 caataaggct gaagaaaagc ttttcaatat gaagggattc gtgggaattg catccgacct
    40921 tatcgaatat gacctcaaaa tcgacagtat tatgaagaac ctgttgggca gggttgctgt
    40981 tgttgaagat attgatgcgg caataaagct tgccagggaa gcaggatatg attataaaat
    41041 agtgactctt tcaggagagg taataaacgc aggtggctca ataacagggg gaagccttca
    41101 gtcttcttca agctctgttc tgaccagaaa aagtgagata gaagctctga aggtcaagca
    41161 taaagaaaag cagcaggagc ttgatgaact cagccggaag cttggggaat tgaaaaaaca
    41221 agcagcggat atgggcgccg acatagagtt caagacggaa atacttcatt ccctgcagat
    41281 agacattaat aattacagga acaggtgtga caatctgaaa aacgaaatcc aggttattca
    41341 ggacagaata tcaataaacc taaaagacct tggagatctg gagaaggagt ataacgaaac
    41401 aaataagata atttctgaaa ataaggaaaa ggcagaggcc gtcgaaaagg aaaaggaagc
    41461 tgttgaggca cagatatcgg agcttcagga gcagatgaag cagttcaagg aagtaaggga
    41521 tgaatataac gatgagataa cagagctgaa aataaaactg gcatctgttg agcataataa
    41581 gaagaccgtt gaaaactcaa taaaaagcat gggggagaga gtaacagaat tagaggagga
    41641 aataaaatca aacaatgctg aaatagaaag acagcaagcg gaaataaaag cattgtcata
    41701 tgatttggaa gaaaagaaaa ctattataga aagaactgta agagaggctg aagaaaagca
    41761 taagcttcat aatgacaagg agaaggagaa atctgccata tgtgaagatt tgagcaagct
    41821 tgaagcagat ataaagaatt acagcaatga tatagctgtt ttgcaggcag accttcataa
    41881 agctgaaatg cagcatgcca gaactgagat agagctggag agcattgaaa tgaggctgct
    41941 ggagacctac gaaatgaatt acagcaaagc ggaaagctac agggatacaa gcttgagtct
    42001 tacatgggct gcaaagcact gtgaggaact aaaaaatgat atcagagaaa tgggcactgt
    42061 aaatgtaaat gcaattgagg aatatgaaaa gctcaaagag cgccatacat tcctcagcgt
    42121 tcaggtggag gatctctcaa aagcccggga aaaccttata gccgttattg ccgaaataga
    42181 agaaaacatg aagcttcaat tcattacgga attcaacaag attaatgaaa atttcaatat
    42241 tgtgttcaag cagctttttg gcggaggaca tgcggaattg gtattgacgg atatggatga
    42301 tgtactgggg tcagggatag aaataatagc caaacctccg ggaaagaagc tgcaaaacct
    42361 attgctgctg tcagggggag agagggcgct gacagctata gccctgttat tcggtatact
    42421 caaaatgaag cctgcacctt tctgcgtcct ggacgaaatt gatgcggccc ttgatgatgc
    42481 caatgttgac aggtttgcag agttccttgt aagcttctcg gcgaccacac agttcatcgt
    42541 tgtaactcac agaaagggaa cgatggaatg tgcggactgc ctgtacggag tgagcatgga
    42601 agacagcgga gtatccaaac tggtatccgt aaggcttgaa gataaagtaa gttaacacgc
    42661 aacagaggaa gaatgcgacg gggacacggg ggacggggac atggggacgt ttcgcgtgtc
    42721 ccactggaag tgggacaggg gaaacgtccc cgtgtccccg tccccatgtc cccgtcgcat
    42781 tattcttcta atacgaaata aaggagaatt tgaatatggc gggattattt aaaaagctgt
    42841 ttaacagagg tcaggagaac gagcaggaag aattacaggc agaagagcag gagcagctga
    42901 ccgatgaaga gatagaagca ataaatgaag aagaaattga aaatgaggaa acggtcgaaa
    42961 atgagcagtc ttccatcttc tcaaggctta agaatggact tgcaaaaaca agaaataata
    43021 taaccggtaa gctgaacggg gttcttgcaa gctttaagag cgtagatgag gagctttttg
    43081 aagagctgga ggaaatattg attacctctg atgttggtgt ttcaacaacc ctgatgatat
    43141 tggacaaact aagaagcaga gttaaggagc agaatataaa agacccctct gaggtaaagg
    43201 aacttctcaa gcaggagata cttgccatta tgggagaaag cacggagagg cttggggaac
    43261 agactcctgc tgtaatactt gttattggtg taaacggagt aggtaaaaca acatctatag
    43321 gaaagattgc ttcaaaaatg aaaaagtccg gcaagaaggt aattattgcc gcagcggata
    43381 ccttcagggc cgctgcgata gaccagcttg aaatatgggg caaaaggatc ggagttgatg
    43441 taataaagca tcaggagggt gctgaccccg cagctgtgat atttgatgca attaactcgg
    43501 caaaatccaa gaaggcagat atacttatat gcgatacagc cggcaggctg cacaacaaaa
    43561 agaacctgat ggaagagctg aagaagatat caagaataat agaaagagaa ttcccggaag
    43621 cgtacaaaga gactcttctc gtacttgatg ccactaccgg acagaatgca atttcccagg
    43681 caaagctttt caaagaggca gcggacataa acggattggt gcttacaaag cttgacggaa
    43741 cggcaaaggg cggaataatt attgcgataa agtctgaatt ggatataccg gtcaagctta
    43801 taggagtagg agagaaagtg aaggatctgc aggaattttc agcttcggac tttgtaaatg
    43861 cattgtttga ggaagacgaa taaatttgtt gacaaatata taatgttaat ataaaatatc
    43921 aatgtaaagg tcataacctt tacattgata tttttataga agcaatgagg tagaaatatg
    43981 tacgacaagc ttgtacaaat agcattgttg tttgattttt acggtcagct gctcacagaa
    44041 aagcagattg agatagttga catgtattac aacaatgacc tctctctggg cgaaatatca
    44101 gagcaacagg gcataagcag gcagggggtt tatgacactt taaagagggc tgaaaggact
    44161 ctttatcaat atgaggaaaa acttggcctg gtggatagat ttctgaagca gaagcaaaat
    44221 atggagaaga taattgaaat gcttgaggaa ttaataacag aagacggata tgacaggaaa
    44281 aatattaaag ataagattaa tgaaataaaa gagtatattg gcaatataat atgagtaatt
    44341 attgaatgca ttgaccctgc gcaggctgaa agatatacag cggggcaaga tgaggtgaga
    44401 tgtttggcct ttgaaggttt agccgaaaaa ctgcagaata cgctgaaaaa gctcagagga
    44461 gccggaaaat taagcgaaaa agacgtaaag gatgccatga gagaggtcaa aatggcactc
    44521 ttggaagctg atgtaagctt taaggtggta aaggaattca taacaaaagt gacggaaagg
    44581 gcggtagggc aagaggtatt ggacagcctg acccccggcc agcaggtaat aaagatagta
    44641 aatgaacaga tgacagagct tatgggaaac accaacagca aaatcatctt ttcaccgaaa
    44701 cctcccacta ttcttatgat ggttggcttg cagggagccg gaaagacgac tacctgcgga
    44761 aagcttgggc tgctgcttaa aaaacaggga aagaatcccc ttttggttgc cggagacgta
    44821 tacaggcctg cagcaatcaa acagcttcag gttgttggca gccaggtggg tatagaagta
    44881 tttgccatgg gcgataaagt gagcccggtg gatatatcaa aggctgcggc tgcacatgca
    44941 ttaaaaaacg gcaacgatgt tgtaataata gacacggcag gacgccttca cataaatgaa
    45001 gagttgatgg atgaactggc ggatatcaaa gaaaatgtta aacctaccga gatactcctt
    45061 gttgttgatt caatgacagg acaggatgcc gtaaatgtcg ccgaaagctt caatgaaaag
    45121 cttggcattg acggtgtgat actgaccaag ctggatggag atacaagagg cggcgcagca
    45181 ttatcagtaa aggcaattac aggaaagccc atcaaatttg ccggtatggg cgagaagctg
    45241 aatgaactgg aaccttttca tcccgacaga atggcttcac gaatcctcgg aatgggagat
    45301 gtgctgagtc ttatagagaa agcccagtct gcatttgatg agaagaaggc cgttgagttg
    45361 gaaaggaaga tcaggactca gcagtttacc ttcgatgatt ttcttgacca gcttcagcaa
    45421 atgaagaaga tggggccgct aagctccgta cttgaaatgc tcccgggaat gaacccaagc
    45481 aagctcaagg gcatggaggt tgatgacaaa gagcttgtaa aggtagaggc aataatcagg
    45541 tctatgacaa aaaaggaaag gcataatcct gatataataa acggcagcag gagaaaaagg
    45601 attgccgcag gcagcggaac cagcatacag gatgtcaaca gagtcatgaa gaactttgaa
    45661 cagtcgaaga agatgatgaa acagtttgcc ggtatggaaa aagatatgaa gaagggtaaa
    45721 atgaaaatgc ctttctttag ataaaaagca ttatgagata attcagtttt gtaaggaggt
    45781 gaattcaatg tcagttaaaa tcagattgag gagaatggga gcaaaaaaag cacctttcta
    45841 tagattggta gttgctgatt caagatctcc gagagatggc aagttcatcg aggaaatcgg
    45901 gtattataac cctatcacag aaccggcaga aatcaagata gatgctgaaa aagcaaagaa
    45961 gtggctggat tgcggggctc aaccatcaga cacagtgagg tcgcttttaa agaaaagcgg
    46021 aataattgaa ggttaatcaa acggaataaa tttcctttag gagtgttgca attgaaagag
    46081 ctggtagaag taatagcaaa ggcccttgtt gaccaccctg aaatggtaaa ggtaagagaa
    46141 acagaaaatg aaagatcaat tgttttggag ctgacagttg ctcccgagga catgggtaag
    46201 gtcataggaa ggaacggaag aattgcaaga gcaataagaa ccgttgtgaa atccagttca
    46261 gcaaggctgg ataaaaaggt aatagtagag atactataaa tattctttta tttttccata
    46321 gtatatgaaa gcacgctttc atatactatg ggcattaatt ctttttgggc ttttgcctga
    46381 ttatttgcgg gagtagatga aaatgctgga atacctgagt ataggacaaa taataaacac
    46441 acacgggtta cggggggcag taaaagtata ccccctgact gatgacatca gcaggtttaa
    46501 aaagctcaaa gaggtttatg tggaagaaaa cgacgggatt gtgaaataca aagtggaaag
    46561 cgtcaaattc ttaagcagtg cagttgccgt aaagcttaag ggtattgact cggaagaagc
    46621 ggcaaataag ctgagaggct tttatattaa ggttgacagg aaatcagccg ttaagcttcc
    46681 caaggatacg tatttcatat gcgatttgat agatttggaa gtgtatgatg aaaaaggcct
    46741 gcttataggt actgtcaagg atgtgctgca gaccggaagc aatgatgttt atgtaattga
    46801 atcttccggg aaggatattt tgatacctgc ccttaaggat atagtcaaga aaattgattt
    46861 ggaaaacaaa aaaatcttgg tagaaatgcc agagggtttg atttagatga agatagatat
    46921 actgactctt tttcccgaga tgtttgaggc cgtattgaaa gagagcatta taggaagagc
    46981 attggaaaag cagctgatag agcttgaatt tcataacata agggattatt cggataacaa
    47041 gcacaggaag gtagacgatt atccctacgg cggcgggctg ggaatgatta tgcagtgcca
    47101 gccgatattt gccgcagtgg aagcaataag cgcaaagctg ggagaaaagc ctcacatcgt
    47161 acttatgagc ccccagggaa gaacctttga ccaggcaaaa gccgaggagc taagcactca
    47221 taagtatctt acaatattat gcgggcatta tgagggaata gatgagagag ttaatgaagc
    47281 acttgtggat gaggaaattt caatagggga ttttgtattg accggcgggg aaattgccgc
    47341 catggcagtg gtggatgcaa cctgcaggct tattccaggt gtacttaagg aggatgcctc
    47401 ttatacggag gaatccttca accggggctt gctggagtat ccgcagtata caaggcctcc
    47461 tgtatttatg ggaatgccgg tgccggagat tttgctttcg ggccatcatg aaaatataga
    47521 aaaatggaga agatatcaat ccttgaaaag gacttgggaa agaaggccgg atctgcttgc
    47581 agaatcagaa ctgtctgaag aggacaggag gcttttggat aagataaaag aggaatgcaa
    47641 ataattctct taagcacttg catcaaggct tgccttatgg tatactttta acgttattac
    47701 gggcgttccg ctgtcaaagc accggtgcaa tgaatatgaa ttgcatgaat tataattgta
    47761 catgaacgtc tatgatggaa ggagggacat tgtgatggat ttgattaaag ttatagaaag
    47821 cgaacagctt agaaatgata tacctgtttt taacgtagga gacactgtta aagtacacgt
    47881 taaggttaaa gaaggaaaca gggaaagaat tcaggtgtac gaaggtgttg ttatgaaaag
    47941 acagggtggc ggactgtcag agacttttac cgtaagaaga atatcttatg gtgtgggtgt
    48001 ggaaagaacc ttccctttac actcacctaa actggacagg attgaagtcg taagaaaagg
    48061 taaggtaaga agagccaaaa tcaactactt gagaggcaga gtaggtaaga gagctaagat
    48121 aaaggaaata agataaaagg ggcttaatag ccctttttgt ttttaattgg agtgatgcat
    48181 atgaacattc aatggtatcc ggggcatatg gccaaagcca agcgaaagat agcggaggag
    48241 ctgaagctgg ttgatgttgt aatagagctt ctggatgcaa gaatacccat gagcagcagg
    48301 aatcctgaag tagatgagat tgtcgggaat aagaaacgga taattgtgct gaataaaagc
    48361 gatctggctg attcttcggt taatgcaaag tggttggttt attttaataa ggaaaacaca
    48421 agggcgatat tggttaattc cttgaaagga aatggactca aggatgtact gtctgcttca
    48481 agacagctta tgaaggaaaa gcttgacagg ctgaaaagca aagggctgct ggttaagaca
    48541 acaagagctc ttataatcgg cataccgaat gtcggaaagt ctacgttcat taacaggctt
    48601 gcgggaagaa gcgtggctca gactggagac aggccgggag tcacaaaatc gaagcagtgg
    48661 ataaaggtga gtaacgagct tgaactgctg gacacaccgg gaatcctttg gccaaaattc
    48721 gaggataaaa gggcgggaat gtatctggcc tttaccggag cgataaagga tgagatattg
    48781 gacgtaaatg aactggctca gaatctgctt caggtattgg cagagaagtt tccggaaaag
    48841 ctgaaaagca gatataagct ggaagacata ccggaaggaa ttgcaccgga agagcttatg
    48901 aaaagcatag gcagaaaaag aggctgcata atagcgggag gagaagttga cgtgctgaga
    48961 gcttccgcaa tgctgctgga tgagttccgg ggagggaaga taggggctat atcattggaa
    49021 tcaccggaag atttttaggg taatggcgat aaggggcata aataatggtg ccaaaaatga
    49081 ttatgtatta gaggggttta agcgagctta ggcccctttc gcatttccag aaattacttc
    49141 gttttcagcg tatcttatgg tataattgga gtatcataag ccttggaaat acggaggtta
    49201 tgggttttat gcctaaaaaa aacgatgact atataagcgt attaggcggt tgcaacatgg
    49261 acattattgg ttttccttac aagaattttg ttcctgaaga ttcgaatccg ggccggatta
    49321 aggctgcttt agggggagtg ggcagaaaca ttgccgagaa ccttgtttgt ttaggcatcc
    49381 ctacaaagct gataagtgca atcggcgatg atatctacgg aaagataata ctggagcatg
    49441 ccgataatat cggccttgat atgaagaatt ctctggtatc gaatcaaaca actacatcca
    49501 catatttagc tatactcgat gaaaaaggag atatgaaggc agctgtttcc aatatggatg
    49561 tactggatga aattaccatt gaattcattc aagaaaagaa gcaagtgata gagaattcga
    49621 agatatgtgt aatagatact aacatgccaa ccgacatcat taattatgtt ttagataatt
    49681 ttaaaaacac ggattttttc ctggatactg tatcaggaac aaaagcccaa aaggtaaaaa
    49741 acaatatcgg agcattccat accataaagc caaacaggat agaagcagaa attctttcgg
    49801 atattaaaat agacagctta ggcgatatga taaaagcctc agagtatttt ctcagccggg
    49861 acgtaaagag agtctttatt acattagggg ctgagggtgt gttctataat gatggaaatg
    49921 ttcataaatt gatacctagc cccaaaataa aagtaatcaa tgcaaccggt gccggagatg
    49981 cattcctggc tgccttggcc ttttgccgct tcaaaaatta cggtattgat gaaagtacac
    50041 gtttttcaat ggcagccgca attttggcat taagctgcga aaaaacaata aatcctaata
    50101 tttctgaaaa aaatgtaata aaaaaaatga aggagatagg attatactga ataaatatct
    50161 ggatatcaat ccggaggcag aaaacgctta aatgaaggtt aaagaacttg cagccggcgc
    50221 aagctttgat tccaatatta agcttgttta taataaagtt agggttggag cacatatcag
    50281 cagatcacac ttttctatct gcttcattac ctttcttagt tctccatttg cttttgcatc
    50341 ggctaatccc aatgtgacct taccccaaat tttgtaccac gctgaatgtt agtccggccg
    50401 attctgggg
//
LOCUS       DAOQUR010000015        49112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_800_length_49112_cov_9.620462, whole genome shotgun sequence.
ACCESSION   DAOQUR010000015 DAOQUR010000000
VERSION     DAOQUR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 49112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49112
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_800_length_49112_cov_9.620462"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..676)
                     /locus_tag="PKI50_04995"
     CDS             complement(161..676)
                     /locus_tag="PKI50_04995"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNT03100.1"
                     /translation="MISIFRINDMIDLPVFDCVSEQKRCTVRDVIIDTKENRLHILIC
                     RERMLGRSLEAIPYKNVKSISENGIRASGNISRMSPRGFLVKQRRYSSCQDILGKLVQ
                     SPRGEPLGIIRDILFDTNTGIIKAYELSEGYFDDFLRGRHIVELEHGHTLTGSNAVIK
                     ENTAKGMFKTK"
     gene            complement(716..910)
                     /locus_tag="PKI50_05000"
     CDS             complement(716..910)
                     /locus_tag="PKI50_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HNT03101.1"
                     /translation="MMSARDCLQRAWNNTMELVRDFEMYSKRIDDGEVAETFKKMAEE
                     QGVMAHDLRELYNRYDNQKK"
     gene            complement(1151..2236)
                     /gene="mnmA"
                     /locus_tag="PKI50_05005"
     CDS             complement(1151..2236)
                     /gene="mnmA"
                     /locus_tag="PKI50_05005"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038016936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNT03102.1"
                     /translation="MQMKKRVVIGMSGGVDSSVAAYLLKQQGYDVIGVTMQIWQDKSK
                     EALEREGGCCSLSAVEDARRVCTKIDIPFYVMNFKEVFEKLVIDYFVDEYLCGRTPNP
                     CIACNKFIKFDALLERSRALEADYVATGHYARIQYEENLKRYVIRKSAAPDKDQTYVL
                     YNLTQEQLRHILMPLGDYNKSQVREIARELDLRTANKPESQEICFVEDNDYGRFISER
                     KGSEIKPGYFVDTQGRRLGTHKGIVHYTVGQRKGLGIAFGKPMYVVEIIPDKNLVVLG
                     DEKEVFSKELIASQMNYIAFEKLEGAINVKAKIRYSAKEAEATVTPIDNERVRVVFRE
                     AQRAITPGQAVVFYQEDILVGGGTIEK"
     gene            complement(2261..2710)
                     /gene="nifU"
                     /locus_tag="PKI50_05010"
     CDS             complement(2261..2710)
                     /gene="nifU"
                     /locus_tag="PKI50_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HNT03103.1"
                     /translation="MYSEKVMDHFSNPRNVGEIEDASGVGEVGNAKCGDIMKIYLKIE
                     DNIIKDVKFKTFGCGAAIATSSIATEMIKGRSVEEALELTNKAVVEALDGLPPAKIHC
                     SVLAEEAVKAAIEDYMKKQGKEVESSVSAGIEPDEEACCSKCERKCH"
     gene            complement(2703..3896)
                     /gene="nifS"
                     /locus_tag="PKI50_05015"
     CDS             complement(2703..3896)
                     /gene="nifS"
                     /locus_tag="PKI50_05015"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNT03104.1"
                     /translation="MDGIRGIYMDHAATTYTKREVLEEMLPFFTEYFGNPSSIHKFGR
                     DVRKHVDTARERVAKALGADPSEIYFTAGGSEADNWAVKGTAYANKSKGNHIITTNIE
                     HHALLHTCEYLEKEGFEVTYLPVDEYGLVSVEDVKNAITDKTILISIMYANNEIGTIQ
                     PIEEIGELAREKGIYFHTDAVQAVGSVKIDVKEQNIDMLSLSAHKFYGPKGIGVLYVR
                     KGVKLINLVHGGGQERGRRAGTENVPGIIGLGKAIELAAENLEESSKRIMGLRDKLIK
                     GVMNKIPYTRLNGHPVKRLPGNANFSFQYIEGESLLLNLDMKGIAGSSGSACTSGSLD
                     PSHVLLAIGLSHEIAHGSLRLSLGAVNTEEDIDYVLEVLPEIVERLRQMSPLYEKVKG
                     GNTNV"
     gene            complement(3898..4338)
                     /locus_tag="PKI50_05020"
     CDS             complement(3898..4338)
                     /locus_tag="PKI50_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT03105.1"
                     /translation="MILSTKGRYGLKIMYELALNYGEGPMPLKEVAQRQQLSETYLEQ
                     LIAHLKKAGLVRSIRGAQGGYELIRKPEEISVGEIIRTLEGPLAPSECVMDDEPECSK
                     AENCVTRLIWEKIMEGINNVVDSITLNDMVNDYYKLKRKKEGEK"
     gene            complement(4602..5957)
                     /locus_tag="PKI50_05025"
     CDS             complement(4602..5957)
                     /locus_tag="PKI50_05025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNT03106.1"
                     /translation="MKISIIGTGYVGLVCGACLAKKGHDVICVDSQREIADRINNSEA
                     VIFEPYLSEYIGEAVAEGRLLATTDIEYGVASSEISIVCVDTPYKNGSIDLNNVEKAV
                     RDIGRILRIKNPYHVICIKSTVIPSATDTLVKNILEEESGKKAGEFGLVMNPEFLREG
                     EAVTDFMNPDRIVIGAYDDRSYEHFKMVYEGYFEAPLLRVSLRTAELIKYASNSLLAM
                     LISYSNEIAAISEAIGGLDAEEVLYGVTLDKRISPQIDGKFIRPEIVKYLKSGRGYGG
                     SCFPKDLRALIAFTESLGYAPKLLRATAEINEMQVGKITDRLEKELNGLEGKKILVLG
                     LAFKQNSDDIRESPAIRLVKLLSEKEAVIYAADPMATEKARKALEGYRGICFLEGYKK
                     TAISFDAAVLMTPWQEYLEFLNEGSSASLKTALLFDGCRALDKYKFNESGIKYMGVGI
                     S"
     gene            complement(5968..6807)
                     /locus_tag="PKI50_05030"
     CDS             complement(5968..6807)
                     /locus_tag="PKI50_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide-diphospho-sugar transferase"
                     /protein_id="HNT03107.1"
                     /translation="MFCTIVTKHRLHQCIAMYNSLKGRMPEAFLAVLAVDRESGRVLD
                     ALKLPGMLVISAEEQENDELRELKGQRSPSEYCWTLKPVLLLYLYERFADVKIFVYAD
                     TDIFFFDNPMKLFRRKDRWHVLLTTHKVNRKTNGGFAAFKRSNDSYKALKWWKDRCIE
                     WCYCKYDNGRFADQGYLDSMSGMFNGVEYIDIPGANAASWNYFKYDFSEMDGNILVNG
                     SRLIFYHFSGLRLKRYADTVALCGAEIPCVVCGIYSKALRDAIKKIEGIDRKVTDYFY
                     MGK"
     gene            complement(6818..7582)
                     /locus_tag="PKI50_05035"
     CDS             complement(6818..7582)
                     /locus_tag="PKI50_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT03108.1"
                     /translation="MKTVILCGGYGMRMREVTEDIPKPLAIIGGRPILWHIMKIYSRY
                     GFNDFILPLGYKGKKIKEYFMNYDWMQNDFILDFSGNENKVYLLKKPEKWKITFIDTG
                     ADAMTGDRIKRIQPFIDEDTFLMTYGDGLCDADIAKLLKYHQEMGVTATLTAVEKKSQ
                     YGILKARDGLAASFGEKTEIEGLINGGFFVLGQAIFDYLDHEGCSFEKDVLPRLAAES
                     RLAVFEHRGFWQAADTSKDLQDMNENWEAISNKLGG"
     gene            complement(7625..8029)
                     /locus_tag="PKI50_05040"
     CDS             complement(7625..8029)
                     /locus_tag="PKI50_05040"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HNT03109.1"
                     /translation="MKSRYEVITISPYKDGRGELKKVFTMDMLGADESVEEIYVLYTK
                     RNSVRGNHYHKKNVEFFSVIKGTATIALRDLETDSTDVFRASSKDNIVIKVPENTAHC
                     FRNDEEEELVILAAATRQYEPGDTDIYPLRLL"
     gene            complement(8049..9113)
                     /locus_tag="PKI50_05045"
     CDS             complement(8049..9113)
                     /locus_tag="PKI50_05045"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT03110.1"
                     /translation="MSINKIIEEDVRDIYLEIGGTLKVIEGSSFLISGGVGFIGSYFM
                     DLLKFCNDNIFMESVTAICVDNFVTGVPERIGHIRHNENFMFLNADISKPIEIDYDID
                     YIINAASIASPSYYRKYPIETLETNVLGLKNLIELGMKKNIKSFLNLSSSEIYGDPDE
                     SHIPTGEDYNGNVSCTGPRACYDESKRLGETMVVNYYKEYKVPIKSARPFNVYGPGLR
                     LYDKRVIPDFFNDAIHKRKIQILSDGTPTRSFCYISDAVKGFMLVLLSEFDGEAFNIG
                     NDRTEINMKGLAELIAGIVGNVAIEYKKSKEKDYLTDNPQRRCPDLSKAKSYLNYYPK
                     VELEAGLGRLYQWYKQESNI"
     gene            complement(9117..10085)
                     /locus_tag="PKI50_05050"
     CDS             complement(9117..10085)
                     /locus_tag="PKI50_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT03111.1"
                     /translation="MEAGLLRNKKVLVTGSNGFIGSHTVSRLVKYGCEVFAAARDASD
                     LWRLDEVKDSIRMIAIDIRDNEAVRLCMARIKPEYVFHMAAYGVDSRQKDYVAAAETN
                     IMGTLNIVMAAGLAGCEKLLNTGTSMQYGNKKGAIKEEEYYSPTNIYGSTKGAATIIA
                     HQAAAGYNLDIVTIIPFGVFGEKEGSHKFFPHVILSALEDGEVSLSPCEQYRDYCYIE
                     NIIDGFMMAAVNKEVRNEIFNIGSGSIFKLKHYVNLIMKRFETGKRVCYGAVEYRKND
                     LWSPQPDLTKIRTRLGWEPRVNLEEGIDRTVEWFRKNHDKYSVKGR"
     gene            complement(10101..11954)
                     /locus_tag="PKI50_05055"
     CDS             complement(10101..11954)
                     /locus_tag="PKI50_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03112.1"
                     /translation="MIHYYCSIASSDYKYKVLALYNSMVKHDKEFNFFIICMGDEALA
                     SFAALKLENTVLIPVSAIESYYPELTKARKDRNDKEYAWTVKPSAFLYIFNNYNYIDH
                     IMWLDGDTMFLSDPGPIYLEWGRHSILLTEERYTGKHAYLSRIYGIYNTGLLGFRRDA
                     NSFECLKWFQSKVNEWCYDRMENGLWSDQMYVNDWPERFKGVAVIKNAGINMTPFILW
                     RFTAEEKRHIDARRLVLFHYYGFRYAGTDDYDLCTYKDWGFPEAVVRSIYMPYISACE
                     AALLQLKRGNAEETEQISETGAAEDKHNSQYCFCTLTTLEYLPKCLALLDSIESNTDS
                     FHIWICCMEDAAYDILTGMGLENVTLVRVSEIEDNKLRAVKKNRKDYEYCWTLKAPFM
                     LHIFEKYENVASLLYLDADIYLFSRPDRCFKALERHTVLLTHHNFSQPYKHLYRKKGR
                     FNAGIIGFKRCGTALSYLKWWRKKCIEWCFDVVSEGRFADQKYLENFLKPYGSAYLAE
                     NIGMNAAVWNLEDKEVHCRGEDIFIGGDLLVFYHFSSFMILGENEFDLWKWDSLKVSS
                     SAKELIYFPYARAVAESIKKIKPYINEISKVYSGMNAWYRASNSAIIENMD"
     gene            complement(12267..13031)
                     /locus_tag="PKI50_05060"
     CDS             complement(12267..13031)
                     /locus_tag="PKI50_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03113.1"
                     /translation="MTVPNASLPTVLCAGTTRLEVKTGGSTECKDLFFECLKVKKVFD
                     EFVLRDCVEGIKFKVCGNPHGDIIKPALILKNCKLSNVEIKDVSTNSDKRLKFSAKCC
                     CDVFGKDAKGNIIPLEVLDVPEGTNLSEGPCGELCFKFSVRRSYPNVTEENFERLLHF
                     LDQGRFELQCLGEAVIDEENNTTTGEILVTNLGAFIAVKFDAEVQLCVPVLGYCQIEE
                     DVAPIDVDFCEEFEMEDIPSFNPPQLGVPIPPYPIS"
     gene            13368..14891
                     /locus_tag="PKI50_05065"
     CDS             13368..14891
                     /locus_tag="PKI50_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03114.1"
                     /translation="MIDINPSAILEDSKGNRYFFFYRDSSIYYREVPIKGDIKDTMLI
                     SQANADFAAVIDADDSIYLTCNSRYKGVLLFIYTNSGWKFEPVVNLHNSSNIFIMDII
                     VQNGSIHIFFSKKLPIANMYNVYHINKNINDKTPYIEYSWRKNSLSEIYSQNIESSYS
                     LLPLRNGIIHYASVWYDGTLYYINYYCYDDSTKSWLHKSLSISYKNQAFIKLIHQNKK
                     INLLCYSNENEGSNIHHFLSKSSSSSEIDFRELGNVRIDTNGMIPLFYSDDKAIQMAW
                     IKDHVFHQYTFDDSAGRWRKAVDLPVTADTSINAVKIMKSLAPLSITKGFFILDKNYN
                     ISRPAEHISRSIPEDKPKEKAQAASIPDMNDYLRQILDEIRDLSDNVKYLNSRIGSSD
                     NRNSKDPTPPKSGTVDKTATEQPTLKKSGFKEKFMKNERLPNYESLLMKQENITTYVG
                     KPGTVNPASDISKDSEKQAFASPNTAAPNNKENWTEDPEEIPYKYNSLMKKIGEFFK"
     gene            complement(14906..15736)
                     /gene="dapF"
                     /locus_tag="PKI50_05070"
     CDS             complement(14906..15736)
                     /gene="dapF"
                     /locus_tag="PKI50_05070"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNT03115.1"
                     /translation="MKFYKYTGLGNDFILINNLRSNIHIDGSLARKMCDRYFGIGADG
                     IVLASPSESCDIRMEIFNSDGSMAEMCGNASRCFAKFCYEKKLVDKESFTVETLAGTI
                     SPRLNIEDGAVKEVTVDMGMPKFGSKDIPFNADLDKVVDYPIRVDGKEYLITSMFMGV
                     PHTVVFTDSIEDEKVISEGRKIEVSDYFPRNTNVNFVSIINRNEIILRTWERGAGYTM
                     ACGTGACASVVAGIASGKLGSIVRVHLRGGDLTVEWKAGEHVQMTGEAKEVFTGEYEI
                     "
     gene            complement(15783..17048)
                     /locus_tag="PKI50_05075"
     CDS             complement(15783..17048)
                     /locus_tag="PKI50_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNT03116.1"
                     /translation="MSTRAQTVWEAYRFPIILFTAIIIGSIIGIAMGADAAVFKPLGD
                     IFLNLMFTVVVPLVFITISSAVANMANMRRLGSILKNLLLVFVVTGIISAIIMIFFVT
                     VIPPAQGVNLNVNAEEPVEKISLANQAVKAITVSDFPELLSRRNMLPLIVFSVLFGFC
                     VTALGEKGKKVAEALDTLSNVMMKMVSFIMYYAPIGLAAYFAALVGEFGPELLKDYAR
                     AMAIYYPVTIAYFFIAFFVYAYYSGGMPAVKRFFKNIVSPAFTSLATQSSIATLPVNL
                     ESARKMGVPKDIREIVLPMGATMHMDGSCLSAILKISFLFGLYGKEFTGIGTFATAIL
                     ISVMSGVVMSGVPGGGLIGEMLIVNLYGFPPEAFPIIATIGFLVDPPATMVNACGDSV
                     AAMMVTRLVEGKEWLAKVIKGEAQGQFTE"
     gene            complement(17093..18346)
                     /gene="lysA"
                     /locus_tag="PKI50_05080"
     CDS             complement(17093..18346)
                     /gene="lysA"
                     /locus_tag="PKI50_05080"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNT03117.1"
                     /translation="MNYYFSDCDTVALAKEYGTPLYVMSEDIIMERLNEIRYSFTEKY
                     QDVQAYYASKAFLTREMARIIKRENMGLDVVSGGELYTAKSVDFPMEKIMFHGNNKTP
                     EEIKTALECKIGRFVCDSIWEIQLIDRLAKEMGVKADILLRITPGVDSHTHKYILTGN
                     IDSKFGIPIPHVNEAVKLSLESEGITLKGLHFHIGSQILENESYIMAVGIMLNLIKKI
                     KDEVGYVAQEFNLGGGFGIKYLDDTEKRKLSYFVDPMMNSIEEFCSNNGIKRPRIFIE
                     PGRWIVGEAGITLYTIGSIKKIPGVRTYAGIDGGFPDNPRPALYQAKYDGIIANKADS
                     PKKERVTIAGKCCESGDILIWDLYVPELQPGDILAVKSTGAYGYSMASNYNRNPKPAV
                     VMLSKGKPRLIVKRQTYEDLLRNEC"
     gene            complement(18679..20016)
                     /locus_tag="PKI50_05085"
     CDS             complement(18679..20016)
                     /locus_tag="PKI50_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNT03118.1"
                     /translation="MKKDMYSFFDMNREQEMKKESPLALRMRPSDLSEFVGQEDIVAP
                     GKLLYRAIKADKLTSLIFFGPPGTGKTTLAKIIANTSKSHFEQLNAVTSGVGDIRRVV
                     EEAKNRKGMYGQRTILFIDEIHRFNKAQQDAMLPYVEDGTVILIGATTENPYYEVNDA
                     LISRSTIFRLNPLKAKDIRKLVERCLTDEEKGLGSYNIEITEEAMAHIVNIANGDARA
                     ALNAVELAALTTERDSDGKIHIDLKTAEDCIQKRAVNYEKKGENHYDTISAFIKSMRG
                     SDPDAVLHYLAKMLYAGEDPKFIARRIMICASEDVGNADPNAIIVATAAANAVHMIGM
                     PEAQIILSQAALYVACAPKSNSAVVGIGKAMKDVENKRTGAVPLHLRNDAFKGARDWG
                     YGVEYKYPHDYEGNFVEQQYLPDEIKDAQYYFPSDNGYESKIKDPKLKNRRIR"
     gene            complement(20124..20669)
                     /locus_tag="PKI50_05090"
     CDS             complement(20124..20669)
                     /locus_tag="PKI50_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT03119.1"
                     /translation="MKILIFGVLLLLVLIAISTYNSLVVKRNRVKNSWAQIDVQLKKR
                     FDLVPNLVETVKGYAKHESETLEAVVKARNQYISAGTPEEMMKANTEMTGALGRLFAV
                     AESYPDLKANTNFMELQGELSELEDKIAYARQFYNDTVLMYNNAIQVVPANIIAGLCG
                     FREEPYFKADESERQNVKVQF"
     gene            complement(20716..22560)
                     /locus_tag="PKI50_05095"
     CDS             complement(20716..22560)
                     /locus_tag="PKI50_05095"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNT03120.1"
                     /translation="MRRRITILLIFLITCALLSVITAYCDDRSYYMSSFKINAQLDSM
                     GNMDIIEEITYEFDGSFRGVYRTLRTAGSDGIEAVEVYKKQPDMLSPFVQDNSERDNT
                     FQLIGEDGGIKLKIFSAAVNESRTFILKYRVLNAAAKFNDIAEIYWKFMGEDTEVKIE
                     NFELVIKIPEGADKEQIKVFGHGPLSGFSEIADSKTVVLRVDELPARNFVEARVLFPP
                     ELIKDSKKVFNRDALAEIMSEEKEFADEANEIRTKARIIVGFSFVYVLFELLMIVYLY
                     FKFDKEYKAKFAGDYFRELPGSYSPAVMAVLWNFGSVKPRDLTATLMDLVRMKYLELI
                     VEKEEVNGLFGSRADNEYIFKLNKEADLMVLSPHEKYAIEWLIFRIGDGERVSLEDIE
                     NSSKTRESAIDFSRDYDIWTGLVKSEADSYSFFDKNTVKGILFGVLTAVIGMIFGGYT
                     AARHDNILGFAILMLVSIILLIYSLTIRRRSKSGVEQFKMWKAFRKFLRHFSSLDKAD
                     LPAVIMWEHYLVYAISLGVAKEVISQLRLVFREEDFNNSHLTYLYYGRYGHIHNYFDT
                     IDNITDSMVKTTESVYRQAMSKASSGSGGGGGFSGGGGGGGGGGAGAF"
     gene            complement(22703..23764)
                     /gene="orr"
                     /locus_tag="PKI50_05100"
     CDS             complement(22703..23764)
                     /gene="orr"
                     /locus_tag="PKI50_05100"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HNT03121.1"
                     /translation="MSYPAIKVNKEKLKHNTKVLAEKCGRLGIDIVPVTKVYCGMPEI
                     AEASVAAGAKMLADSRIENIKKLKELNAEKMLLRIPMLSQVDEIVEHVDISLNSEYEV
                     IKALSDRAQKRGKVHKIILMADLGDLREGVWYKDAIKFAGRIVKLPGIKLVGLGTNLT
                     CYGAVIPSVENLGLLAALAEEIEKKYDIKLDIISGGNSSSLHLVEKNEVPKGINQLRL
                     GEAIVLGNETAYGERIEGTYGDVFTYMAEIIELKEKPSVPVGETGVDAFGEKPVFVDR
                     GIRKRAILASGRQDLKADGIQPRDKDAIVIGASSDHLIIDVSDCGREYKVGDIMEFDL
                     GYGALLAAFTSEYVEKIIE"
     gene            complement(23768..25123)
                     /locus_tag="PKI50_05105"
     CDS             complement(23768..25123)
                     /locus_tag="PKI50_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HNT03122.1"
                     /translation="MKISVLVAEIGSTTTVVNAFDNIMSPCPKFLGQGQAPTSVYNGD
                     VTIGLKGAVDDLKRNLGAPELEWNEFLATSSAAGGLRMTVHGLVYDMTVRAAKEAALG
                     AGAIIKHVTAGRLRRTDLKRLEDVRPNIILVAGGVDYGERDTALHNFEMIASMGLGIP
                     VIYAGNIENQEEVRLIAEETNTRLYIVENVYPKVDMLNVEPTRKVIQEVFEEHIIHAP
                     GMSTVRDMVRGPIIPTPGAVMEAARLLKEHLGDLVVFDVGGATTDVHSVTEGSEEISL
                     MLISPEPAAKRTVEGDLGVHVNIPHIVEKIGLENLKKEFPEAEELVNNAKPIPETERE
                     KAFVERLTKEAVLTSLERHAGKLRFLYGPTGKKTVAEGKDLTNVKYIIGTGGALTRLP
                     GRIDILKSVGLQGKGTELYPGKEAKVLIDNHYIMASLGVLSKRFPEAALALMKESLGV
                     N"
     gene            complement(25113..27317)
                     /gene="oraE"
                     /locus_tag="PKI50_05110"
     CDS             complement(25113..27317)
                     /gene="oraE"
                     /locus_tag="PKI50_05110"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HNT03123.1"
                     /translation="MKLDPNKKLDIKELLQDLESYRPRRRGWHWREGRNKNRKIGDFE
                     YHETSEGLKNYIPLPGSRGFNYIDPQPDCVVTTEIASGRFEDDIRRMRMAAWHGADHI
                     MVIRTMGQSHIDSLIEGTTQGIGGIAVTRKQCRATRKALDMIEDEVGRPINFHSYISG
                     VAGPDIAVMFAEEGVNGAHQDPQYNVLYRNVNMVRSFVDACEAKKVMAWADILQIDGA
                     HNANATAMKAWKVRPELMVQHAINSIFSRKVGIKPENIALSTVPPTAPPAPRMRIDLP
                     YAVALRDFFSEYKMRAQQNTKYMESDMREATVTHTLDLLISRLTSADIQSTITPDEGR
                     NVPWHYNNIAAVNTAKQALNGMDGLREMVKLDREGPLGKEVRELKERAVLFMEEILET
                     GGYFKAVENGFFIDSGYYPERNGDGIPRQIDGGIGVGMIFERDKDYFAPVSVHYGYNN
                     IPSEFKKASDAIGNDTFEDPSKIIYIDELDEYDNVNTRLADLEKYYNTNTVKPEVEWR
                     ADGVVATTIFLPLDERTAEFAAIKCGEKLGLEDVTVVHKQALHPSEGTLVEIKGKVIF
                     DINVNELVIPEKVQTLSEEEIREDISAKPMFVVAATIGNDEHSVGMKETLDIKHGGIE
                     GFGIKYLYLGTSCPVEKLVDAAIETNADAILASTIITHDDVHTKNMRKIHDLCVEKGI
                     RDKVMLVVGGTQVTNEIAVEAGMDAGFGRGTHGIDVASYLVKRRREKHSNEN"
     gene            complement(27332..27688)
                     /locus_tag="PKI50_05115"
     CDS             complement(27332..27688)
                     /locus_tag="PKI50_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HNT03124.1"
                     /translation="MAVKRADDYEIRRKHLAELSDEELKKRFWELAEKIVDPMLALGY
                     KNTTPAIERSILLRMGFSSLEVKPIVEGVMRKGLMGKGAGNVVWRLSKRLGVSVREAG
                     VGLAEGKYWDDVDALF"
     gene            complement(27703..29124)
                     /gene="ortB"
                     /locus_tag="PKI50_05120"
     CDS             complement(27703..29124)
                     /gene="ortB"
                     /locus_tag="PKI50_05120"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HNT03125.1"
                     /translation="MSKDMSYAAVMARKNEIMKQAIGIDYSVFESGGISFDYERMMRE
                     AGYTLEEMQQIQAETSVGNTPVVELKNLTALARKLAPKGKGARIFIKDEAANPSGSFK
                     ARRAATAIYHAKKHGYKGVVTATSGNYGAAVASQAAKRGLKCIVVQECYDSRMVGQPE
                     ILEKQRACEAYGAEVVQLTVGPELFYTFLTLLEETGYFNASLYTPFGIAGVETLGYEL
                     AMQFREKIGRDPDKVVITHAGGGNVTGTARGLLKAGAKDVEVVGASVDLSGLHMASDR
                     DFNRKSFTTGHTGFGIPFATWPDRADVPRNAARPLRYLDRYVLVKQGEVFYITEALSV
                     LEGLERGPAGNTSLTAAFALAQEMDKDQIIVVQETEYTGAGKHPLPQLTFAKQNGIEI
                     LFGNPDRDEPGKNIILPAHPSDIKVRDYDIDKARASYIKNCINNCKVTEITKEDFEFL
                     VRDAKTDDSFVEGVLAELNVKIK"
     gene            complement(29117..29428)
                     /gene="ortA"
                     /locus_tag="PKI50_05125"
     CDS             complement(29117..29428)
                     /gene="ortA"
                     /locus_tag="PKI50_05125"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HNT03126.1"
                     /translation="MISAKKGDWVQIRQVVLKPEERAPQVPEDTKKMPLELWVKGIAQ
                     EDAKIGSTVEIVTATGRKVRGELTAVNPRYIHDYGDFVPELLKVDMQLKEILFGGDSN
                     E"
     gene            complement(29686..30732)
                     /gene="ord"
                     /locus_tag="PKI50_05130"
     CDS             complement(29686..30732)
                     /gene="ord"
                     /locus_tag="PKI50_05130"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HNT03127.1"
                     /translation="MRKENVKVIIWGLGAMGGGMADMLLKKKGVEIVGAVGRGAKIGK
                     SMYDYIKTERGSNKDVIIGSYEDVIKENAADVVLICTDSFTKGVFDKIKYCLEKKINV
                     ISSAEQMAYPKAAEPELTKQLDEIAKANGVTVLGTGINPGLIMDLLVVAFTGACEEVE
                     HIVARRVNSLSPFGPAVMEEQGIGITVDEFKEGVRTGKLAGHVGFAESIGMIADAIGW
                     KLSDFNQSMEPIVTEVDRKSPYGFAKAGDVAGCAMKGYGYVDGELKIEMDHPQQIEPH
                     LGGVETGDYIIIKGTPNVNMANKPEIPGGIGTIAMCVNMIPHVINARAGLKTMIDLPV
                     PRAIMGDMREMIEE"
     gene            31137..31934
                     /locus_tag="PKI50_05135"
     CDS             31137..31934
                     /locus_tag="PKI50_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNT03128.1"
                     /translation="MYDFHVHSHFSSDSTASMQSMAAKAKCIGLKGICFTDHYDLEYQ
                     STEISFEFSYDRYFDEINSLKANSSENLEIYTGLELGMQPHLSAENEALLKGRSFDFI
                     IGSIHCVNKKDIYCRTFLEGITENEGIRNYFDDMLQCIEEFKNFDILGHLDAVRRYLY
                     NKDNSFSLDLYEESVRNVLSKLVHSNKGIEVNTAGLRYCLSSFHPLPDILKLYRLLGG
                     EIVTVGSDAHKPDDLGYGIKNALSVLSELGFKYYAIYKDRKPIFIKI"
     gene            complement(32126..33532)
                     /locus_tag="PKI50_05140"
     CDS             complement(32126..33532)
                     /locus_tag="PKI50_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT03129.1"
                     /translation="MSLIYKSIIDTILKNIDIGIHVVDSSGKTIIYNQAMADMEGLDI
                     NEVMGKSILDIFPSLTTETSTLLTVLKMGKPIYNKYQTYLNNKGKQISTLNSTIPIRI
                     SQGKIGALEIAKNITKEKELSDKLVYLHQELASKDIDGSSLKGYTFRNLIGEDEKFLN
                     AVEIARKTAMSSSTVLIYGETGTGKELFAQSIHNESSRKHKPLIAQNCAALPESLLEG
                     ILFGTVKGSFTGAINRPGLFEQANGGTLMLDEINSMGHQLQAKLLRVLQEGNIRRIGG
                     LSDIPVDVRILATTNEDPLVLLQKGILRKDLYYRLNVISVSIPPLRERKTDIMLLTDH
                     FIKKYNNILQKDIWYVSNEVKEAFLNYNWPGNVRELENIIESAINMVNRGHVIKPEHI
                     SQEMCEVMFKAGKNNHYLNLNEDQPLDRIVAEVEKGLILEALQKCENNISRSAEMLGL
                     KRQTLQHKLKKYDINIKE"
     gene            complement(33638..34879)
                     /gene="glyA"
                     /locus_tag="PKI50_05145"
     CDS             complement(33638..34879)
                     /gene="glyA"
                     /locus_tag="PKI50_05145"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNT03130.1"
                     /translation="MKIDRIKACDSEIAEAIIKELDRERNKIELIASENFVSPAVLEA
                     MGTVLTNKYAEGYPGKRYYGGCEYVDIVEDIARERMKALFGAEHANVQPHSGSSANQA
                     VYFTVLEPGDTVMGMDLSHGGHLTHGSPVNVSGKFFKFIHYGVSQNTEMIDYDNVRAI
                     AKECRPKLIVAGASAYSRRLDFKAFRDIADEVGALLMVDMAHIAGIIAAGLHQSPVPY
                     ADFATTTTHKTLRGPRGGAILCKQQYAKAIDKSVFPGMQGGPLMHVIAAKAVCFREAM
                     SEEFRGYIEQVLKNASVLADSLTAKDIDIVSKGTDNHMMLLDLRRKGITGRDAERLLD
                     DIGITTNKNTIPFDPQSPFITSGLRIGTPAVTTRGFKEDEMKEIAEIISAALSAPESK
                     AECISRVKALCGKHPLYPEFE"
     gene            complement(35026..35352)
                     /gene="trxA"
                     /locus_tag="PKI50_05150"
     CDS             complement(35026..35352)
                     /gene="trxA"
                     /locus_tag="PKI50_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNT03131.1"
                     /translation="MSKNIKIVNQGNFDSEVLKSNKPVLVDFWAAWCGPCRMVAPVID
                     QLADEYEGKIDIAKVNVDENPELAGRYDIMSIPSVFLFDKGSKVDGVIGARPKQAFDD
                     MLKKHI"
     gene            complement(35460..35900)
                     /locus_tag="PKI50_05155"
     CDS             complement(35460..35900)
                     /locus_tag="PKI50_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNT03132.1"
                     /translation="MREIGKVVKIEGNQATISFTRGTACGECGKCQVGKDKLEMMMTA
                     ENDVGAQVGDEVEIELENMNFMTAVTIAYGFPLLALTAGIFGGYYGSLALGLEDNSAQ
                     AIGVALGLAALAVSYLIIKFKDKSIKKMKKFKPVILSIRTKDSI"
     gene            complement(35919..36191)
                     /locus_tag="PKI50_05160"
     CDS             complement(35919..36191)
                     /locus_tag="PKI50_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNT03133.1"
                     /translation="MDNKEISKEDILNRMKKIEGQAKGIQKMIEEDKCCGDIMVQISA
                     IRSAINKVGGLIMDRYIKECLKESLKDENSDKSIEEIIETIVRYVK"
     gene            complement(36363..37112)
                     /locus_tag="PKI50_05165"
     CDS             complement(36363..37112)
                     /locus_tag="PKI50_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNT03134.1"
                     /translation="MNAFSRTELLIGKDGIEKLKKSTVAIFGIGGVGSYTAEALCRCG
                     IGRLVLVDYDNICLTNINRQIHATRKTVGLPKVEVMKERLTDINPEADIVVHKKLYNE
                     ETSEMLLLPEYDYVVDAIDMVSSKISLVMKCIEMNIPVISCMGAGNKLDPTKLVITDI
                     YKTSVCPLAKVMRYELRKRGVKSLKVVYSKEEPISPKKDVADCKTNCICSNKETANCT
                     KRRQIPGSISFVPSAAGLIIASAVARDILDL"
     gene            complement(37227..38996)
                     /gene="aspS"
                     /locus_tag="PKI50_05170"
     CDS             complement(37227..38996)
                     /gene="aspS"
                     /locus_tag="PKI50_05170"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNT03135.1"
                     /translation="MARINRTHMCGELNMDNLGEKVTLAGWVQRRRDLGGLIFIQLRD
                     RSGIIQCTVNKDATPDVFAEAFKVRSEYVLKISGEVIKRDENAINPKMPTGYIEIAVS
                     SLEILSRAETTPIYIEDDLNASEQLRLKYRYLDLRRPHMQKFLFTRHKIAKVTRDYMD
                     ENGFVEVETPILIRTSPEGARDYLVPSRVQPGNFFALPQSPQLFKQLLMVSGFDRYFQ
                     IAKCFRDEDLRADRQPEFTQIDMELSFVEKEDVMEVNEGLLKRLFKEVLDVDIQTPFK
                     RLTYKEAMEHYGSDKPDTRFGLKLKDIGDLVANSEFKVFTETRANGGIIRAINAEGLG
                     AKLSRKDIDSLGEFVKTYRAKGLAWINITSEGVKSPIAKFLKDYELNGIIDRVGGKLG
                     DIIFIVSDKPKVVFDSLGQLRLELAKRFGLIDNTKYDLLWVTEFPLLDYDEEENRYVA
                     MHHPFTSPMDEDLELLDTDPLKVRAKAYDVVLNGYELGGGSIRIHRQELQEKMFKLLG
                     FTQEQAWSKFGYLLEAFKYGTPPHGGLAFGLDRIVMLFTGTDNIRDVIAFPKTQTASC
                     LMTNAPSAADPKQLEELHIEVKI"
     gene            complement(39008..40264)
                     /gene="hisS"
                     /locus_tag="PKI50_05175"
     CDS             complement(39008..40264)
                     /gene="hisS"
                     /locus_tag="PKI50_05175"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNT03136.1"
                     /translation="MLTQAPKGTKDVLPSESYKWQYVEDLIREICALHGYKEMRTPIF
                     EHTELFERGIGDTTDVVQKEMYTMKDKGGRSITLKPEATAPFVRAYIEHSMFNDIQPV
                     KVFCTTACFRYEKPQSGRLREFHQFDIEAFGSSDPSVDAEIINIAMILYGRLGVKNLE
                     IRINSVGCPKCRAEYNKKLREYLSGKIDKLCETCKDRFHKNPMRVIDCKEESCKEQLA
                     DVPYMLDNVCSECGEHFEGLKRSLEALGLEYVIDPQIVRGLDYYTKTAFEIVSRDIGS
                     QGTVCGGGRYDGMIEECGGPSTPGIGFGLGLERLLLVVENQGIKIPEPVYMDVYIANI
                     GKEAAEEALRQVNLLRKEGIKAEKDYMGRSLKAQMKYANKINARYVAVLGEEEIKSGK
                     IKVKDMNKGSEEEISLDALAGYIKNM"
     gene            complement(40270..41763)
                     /gene="hemZ"
                     /locus_tag="PKI50_05180"
     CDS             complement(40270..41763)
                     /gene="hemZ"
                     /locus_tag="PKI50_05180"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HNT03137.1"
                     /translation="MIEIRLINHEYYNEAADIVRMYFGKCEIVRSEGALADSQSFLQD
                     TGILITSRLDINGEIAVCVSTYLEGQKHEEAVEKSGIRAADGNEAKRLVKKSMYKLLA
                     AVFKREYPWGVLTGIRPVKIVHELMNNAVSTTDIPERLAEEYLVSRDRAELAVRIADI
                     ERPFVYPYNNREISIYIGIPFCPSRCEYCSFTSNSIKAYRGYVEPYMEKLTEEIKRVS
                     EFLNTSGFKVQTIYIGGGTPTALNAQQLERLIKCIGNSFGRQECEFTCEAGRPDSITK
                     EKLQVLYENNIGRISINPQTMNDDTLMRIGRAHDPGQVIESFRLAKEMGFGNINMDII
                     LGLPGETMTELAHTLEEIKKLDPESVTVHTMALKRASVLNEKYMKSALPDDENVSRMM
                     EYTKGFLGDMGMHPYYLYRQKHMVQNLENIGFSKKGFECIYNMQIIEERQTIVAFGAD
                     AVTKVVINSENRLERQHNIKDIKLYIENIEAMIRKKLDVLEQILSEE"
     gene            complement(41772..41954)
                     /locus_tag="PKI50_05185"
     CDS             complement(41772..41954)
                     /locus_tag="PKI50_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03138.1"
                     /translation="MNKSSKKILASKHKNRMYQEYNELIDMIESGMEDFEIATELGIA
                     SGIVSEVKNQIYNDDI"
     gene            complement(42032..42652)
                     /locus_tag="PKI50_05190"
     CDS             complement(42032..42652)
                     /locus_tag="PKI50_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT03139.1"
                     /translation="MMKFKSLALGIYQTNCYIVYDEKTKETAVIDPGGDFPAIKSYIE
                     ANGLNIKYIIITHAHGDHIGALKELKEYSRALVCIHREDSEMLKSKTMNFSGMLGGAA
                     IEMTADKYLEDGEVLELGDTKLNIIHTPGHSRGGISIYCDKVLFSGDTLFKNSVGRTD
                     LYGGSMTELVSSIREKLFTLPDDTMVYPGHGPSTALLHEKQENSFV"
     gene            complement(42673..43122)
                     /gene="dtd"
                     /locus_tag="PKI50_05195"
     CDS             complement(42673..43122)
                     /gene="dtd"
                     /locus_tag="PKI50_05195"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT03140.1"
                     /translation="MRAVIQRVNSGSVAVDDKIVGSIGKGFVVLLGVNEKDTEADVLY
                     MVDKTVNLRIFEDDNEKMNLSLLDIKGELLVISQFTLYGDCRKGRRPNFMSAARPEKA
                     EELYNEFVKKCRELGVKTETGTFQAYMKVNIENDGPVTVIVDSEKII"
     gene            complement(43147..45315)
                     /locus_tag="PKI50_05200"
     CDS             complement(43147..45315)
                     /locus_tag="PKI50_05200"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNT03141.1"
                     /translation="MLEDLLKIIKKYNPTGDAEIVERAYNYAARAHDGQKRVSGEDYI
                     FHPLEVAKILAELNMDNITIAASLLHDVIEDTKCTYEDCKNLFGEEIAMLVDGVTKLG
                     KLEYKTKEEQQAESLRKMFIAMAKDIRVIMIKLADRLHNMRTLRFLSPEKQKEKARET
                     MEIYAPIAHRLGISKVKWEMEDLALRYLEPKGYYELVDKVAKKRKEREDEINQVMEVL
                     KGKLEEVGIEAHIEGRPKSFYSIYRKMYYQNKTFEQIFDLTAVRIIVDTVKDCYGVLG
                     IVHTLWKPIPGRFKDYVAMPKPNMYQSLHTTLLGFGGQPFEIQIRTWEMHRTSEIGIA
                     AHWKYKEGKTSQSEFDEKLKWLRQMLEWQNEIKDTREFMETLKVDLFTDEVYVFTPKG
                     DVVDLPVESTPIDFAYKIHSQIGNRCIGAKINGRIVPLDYKLQNGDIVEVITSAVANG
                     PGRDWLKIVKSSQAKNKIRQWFKKEKREENIQKGKELLEKEIRRNGYTTVQLMRPEWV
                     EAIYKKFSLHSLEDMYASLGYGGLTPNQIIAKLKEEFRKTQKLEEITEDSIERQAEKA
                     QERKKRYNSTGVKVKGVENILIRFSKCCNPVPGDEIVGYITKGRGVSVHQKDCPNVAG
                     LLNEEERFIEVEWSSQTKESYSADVEIRAMDRKGLLAEVTTIINESKINISSFHCRIT
                     KDKMAIINFILDITDIEQLNKLIRKFRRIEGVVDVFRAKQ"
     gene            complement(45457..45969)
                     /locus_tag="PKI50_05205"
     CDS             complement(45457..45969)
                     /locus_tag="PKI50_05205"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNT03142.1"
                     /translation="MDLIKYIRTIENFPKEGISFKDVTTLLKEGDAFKYAVKSIADNL
                     SNYDFDIIVGPEARGFIFGAPAAYELGVGFVPIRKPGKLPAETACYKYELEYGMDALE
                     IHKDAIKKGTRVAVVDDLLATGGTVLSAVRLVEQLGGEVVHIAFLIELEFLKGRENLK
                     GYDVSSIIKY"
     gene            complement(45988..47688)
                     /gene="recJ"
                     /locus_tag="PKI50_05210"
     CDS             complement(45988..47688)
                     /gene="recJ"
                     /locus_tag="PKI50_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNT03143.1"
                     /translation="MNKRWTAYSKSMDSLNPIIEEFQLPEIIIRAMINRGIDTVPKAR
                     EFFNTSLCSLHDPYLLMGMEQAANRIFKAVRSNEKICIYGDYDVDGITSTAVMIKTLR
                     KLGADGVYYIPNRLEEGYGMNLNSIDRIKEMGVSLIVTVDCGIKSCEEVDYAKELGID
                     IIITDHHECGEELPDALSIINPHQPGCGYPFKDLAGVGVAFKLAGALLCRAGSPGFAE
                     GLLDIAAIGTIADVVPLLGENRIIVKNGLASIQNTDSQGIKALLAVCGLKGKELNSYN
                     VAFMLAPRINAAGRIDDAGECVELLLTDNAEKAMETAAKLDSDNKMRQSIENGILNCA
                     EAMIGESVDLDRDKVLVLCREDWHIGVIGIVASRLADKFYLPAFIMVCDGEFCKGSAR
                     SIPGFNIFEAMSRHGYLFERYGGHEMAAGFTIRTEKIDELRERMNFEVENTLGEDKLI
                     PEIFVDYKLEPKDVTLDTAKLLKLMEPFGTGNTVPLYVFRELSVTSFKGVGNDSRHLS
                     LKVFDGEKEISCIGYNLGNMQKILQIGEKIDIICSIENNIWNGTESVQLNIKDIKLIN
                     "
     gene            complement(47726..48070)
                     /locus_tag="PKI50_05215"
     CDS             complement(47726..48070)
                     /locus_tag="PKI50_05215"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNT03144.1"
                     /translation="MQFTIVISLVFAVIIAFFAVQNAGVVVINFLWYKVSLSQAVVIL
                     CSALIGVLIMLPFDIIRRIKYKFKLRELTGENKKIKEELKRIESEKLPAKEPVMQEKP
                     QTIEDIDKMDIS"
     gene            complement(48431..>49112)
                     /gene="secF"
                     /locus_tag="PKI50_05220"
     CDS             complement(48431..>49112)
                     /gene="secF"
                     /locus_tag="PKI50_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNT03145.1"
                     /translation="ATITHAGQNKDQVIVRTKINFDNEKRAEIIKAFSDKYSIESNNI
                     SFETIGPTVGKELAMQSLIAVMLAFAGIMIYVSFRFEWRFGLASLSALIHDTAMMVAA
                     YAVFQIPVNSTFIAALLTIIGYSINDTIVIFDKIRENLKNEHSKKIDYDNLVNVSVTQ
                     TLARSINTSLTTLITIFALYFLGVPAIRDFAFPMIIGIVSGCYSTIFIASPLWVILRR
                     RFKVSRAR"
BASE COUNT        12519 a        12552 c         8380 g        15661 t
ORIGIN      
        1 cggggacacg gggacgtttc gcgtgtccca ctggaagtgg gacaggagaa acgtccccgt
       61 gtccccaaaa cgtccccgtg tccccgcgtt cccgtgtcgc attcttcctg ttttccgtag
      121 tatatataat ttatgattcg gatatgtata aaattcctgc ttatttggtt ttaaacattc
      181 ctttagcagt gttttcttta ataacagcat tgcttcccgt aagtgtatgt ccgtgttcca
      241 gttctactat atgcctgccc ctgagaaaat cgtcaaaata cccctctgac agctcatatg
      301 cctttatgat tcctgtgttt gtgtcaaaaa ggatatctct tattataccc agaggctccc
      361 ctctgggact ttgcacaagc tttcccaaaa tgtcctgaca gctactgtaa cgcctctgct
      421 ttaccaaaaa gccccttgga ctcatccggc tgatattgcc cgaagcccgt atgccattct
      481 cggaaattga tttgacattt ttatagggta ttgcttccaa tgacctgccg agcatcctct
      541 ccctgcatat taggatatgt aatctgtttt ctttcgtgtc aatgattaca tctctgacag
      601 tgcatcgctt ttgttcggat acacagtcga ataccggcag gtcaatcatg tcatttatcc
      661 tgaaaatact aatcacctca catttttaca aagaaaaaag accgattccg gtcttttact
      721 ttttctggtt gtcataccta ttataaagtt ctctcaggtc atgtgccata acgccttgtt
      781 cttcagccat tttcttaaag gtttccgcca cttctccgtc atctatcctc ttggaataca
      841 tttcaaaatc tctgaccaac tccattgtat tgttccaggc tctttgaaga caatccctgg
      901 cgctcatcat attgttgctg ttcaagcttt tgctgtccac tataacacct ccgcagcttt
      961 tattatgtca ttattttagc ttaaacagcc aatattattc ataaagtatt gaacgataaa
     1021 ttctgttttt tattcggcac aattttgcgc gaaagattcc ttttgcgcaa tgtactgccc
     1081 tgataaagac ttcatctgtc atgtttgtca aggaataaca gtagaaaagg aatataaata
     1141 cctttctttt tcacttctct atagttcctc ccccaaccag tatatcttcc tggtagaaca
     1201 caaccgcctg accgggagtg attgctcttt gagcttccct aaacactact ctcactcttt
     1261 cattatctat gggtgtgact gttgcctctg cttcttttgc actgtatctt atctttgcct
     1321 tcacgtttat tgccccttca agcttctcaa aggcaatata gttcatctgt gatgcaatta
     1381 attccttact gaaaacttct ttttcatctc caagtactac aaggttttta tcaggaatta
     1441 tttcgactac atacataggc tttccaaagg ctattcccaa gcccttgcgc tgaccaaccg
     1501 tgtaatggac tattccctta tgagtaccga gccttcttcc ttgtgtatcc acaaaatatc
     1561 ccggcttgat ttcgcttcct tttctttcgc ttatgaacct tccgtagtca ttatcttcaa
     1621 caaaacagat ttcctggctt tcaggcttgt tggccgttct aaggtccaat tccctggcta
     1681 tctccctgac ctgactcttg ttgtagtccc ccaggggcat aagtatatgc ctgagctgtt
     1741 cctgagtcag attataaagc acatatgtct ggtctttatc gggagctgcg gattttctga
     1801 taacgtacct cttcagattt tcttcgtact gtattcttgc ataatgccca gttgcaacgt
     1861 aatctgcctc caaagccctg gagcgctcca aaagcgcatc gaattttatg aatttgttgc
     1921 aggctatgca cggattcgga gtccttccgc acagatattc atcaacaaaa taatcaataa
     1981 ccagcttttc aaatacttct ttaaagttca taacataaaa gggtatgtca attttagtgc
     2041 atacccttct ggcatcctcc acagcagata aagaacagca gcctccttca cgctcaaggg
     2101 cttccttgct cttatcctgc catatttgca tggtaactcc aattacatca tatccctgct
     2161 gtttcagaag gtaagcggcg acagagctgt caactccgcc gctcatacct attacaactc
     2221 gctttttcat ttgcattcaa ctcctaatga gcatacaggg ctaatgacac ttccgctcac
     2281 atttactgca gcatgcttct tcatcaggct caatcccggc tgatactgag gattccactt
     2341 ccttaccctg ctttttcatg taatcctcaa ttgcagcttt aacggcttct tcagccagca
     2401 ctgagcagtg tatttttgcg ggaggcagtc cgtcaagagc ctcaactaca gctttatttg
     2461 taagttcaag ggcttcctct accgacctgc cctttatcat ttcagtggca atgctgcttg
     2521 tggctattgc ggcaccgcat ccaaaggtct tgaacttaac gtcctttatg atgttatctt
     2581 ctatcttcag ataaatcttc attatatcgc cgcacttggc attgcctact tcgcctacac
     2641 cgctggcatc ctctatttca ccgacatttc tcggatttga aaaatgatcc atcacttttt
     2701 cactatacat tagtatttcc ccccttaact ttctcataca acggagacat ttgcctcagc
     2761 ctttccacta tttccggcaa tacttccagg acataatcta tgtcctcttc ggtatttacg
     2821 gcacccaggg acaatctcag cgatccatgg gctatttcat gggacagacc gatggccagc
     2881 agcacatggg aaggatccaa tgatcccgaa gtacatgccg agccgctgga accggcaata
     2941 cctttcatat ccagattcag aagcagtgac tctccttcaa tatactgaaa actgaaattg
     3001 gcgttccccg gaagcctttt gaccggatgt ccgttcaacc ttgtataagg tatcttattc
     3061 attacgccct ttatcaattt atccctcagc cccattatcc ttttgctgct ctcttccagg
     3121 ttttcggcgg caagctcaat ggcctttccg agtcctataa tacccggaac attttccgta
     3181 cctgctcttc ttcctctttc ctgaccgccc ccatgaacaa ggtttataag ctttactccc
     3241 tttctcacat aaagtacccc tataccttta gggccataga atttatgtgc ggaaagcgag
     3301 agcatgtcaa tgttctgttc ctttacatca attttaacac tccctaccgc ctgcactgca
     3361 tcagtatgga agtatatgcc cttttccctt gccagctccc caatctcttc tatgggctgt
     3421 atcgtaccga tttcattatt ggcatacata atggaaatca gtattgtctt atcagttatt
     3481 gcgtttttta catcctcaac gcttaccaaa ccatactcat ctacaggcag gtatgttact
     3541 tcaaagcctt ccttttccag gtattcacaa gtatgcaaaa gggcgtggtg ttcaatgttt
     3601 gtagttatta tatgattgcc tttgcttttg ttggcatatg ccgtgccttt taccgcccag
     3661 ttatctgctt cagagccccc tgcagtaaaa tatatttctg aagggtccgc acctaatgcc
     3721 ttggcaaccc tttccctggc agtatccaca tgcttgcgca cgtctcgtcc gaacttgtgt
     3781 atgcttgaag gattcccaaa gtactcggta aagaaaggaa gcatttcttc cagtacttct
     3841 ctttttgtat aggttgtagc tgcatgatcc atgtaaattc ctcttatgcc gtccatatta
     3901 tttctctcct tctttctttc gtttaagctt ataatagtca ttcaccatat cattcaatgt
     3961 tattgaatct accacattat tgatgccttc cattattttt tcccaaatca atcttgttac
     4021 acagttttca gcttttgagc actccggttc atcatccatt acacattccg acggggccag
     4081 ggggccttca agagtcctta ttatttcccc cactgaaatc tcttcgggtt ttcttatcag
     4141 ctcatatccc ccttgagcac ctctgatgct tctaactagc ccggcctttt tcagatgggc
     4201 tataagctgt tccagatagg tttcggaaag ctgctgcctc tgagctactt ctttcagggg
     4261 catcggccct tccccgtagt tcaaagcaag ctcatacata attttgagtc catatcttcc
     4321 cttggttgat aatatcatat tatcagctcc ttattaattc ctacctatta agtcggattt
     4381 caatttaagg ttaccatatc agaaaaacat tgtcaatatt aatttctact tttttagtcg
     4441 gaattatccg tgccattgcc ggcctcaaga taacaaaagg tgcggcttgc gccgcacctc
     4501 tttatataaa ttgcctcccg atatattgtt atatcgaaaa gcaacagctg tactataata
     4561 catactatgc aaggctttta aaaatgttaa ccgtgaagca gttatgatat gccgacaccc
     4621 atatatttta tgcctgactc attaaactta tacttgtcaa gggctctgca gccatcgaac
     4681 aacagtgccg ttttcaagga tgccgaactg ccttcgttca aaaattccag atactcctgc
     4741 catggtgtca taaggactgc cgcatcaaag cttatggctg tttttttata tccctccagg
     4801 aaacatatcc cccggtatcc ttcaagtgct tttcttgctt tttccgttgc catgggatct
     4861 gctgcatata ttaccgcctc tttttcagaa agaagcttca caagccttat agccggagat
     4921 tctcttatat cgtcgctgtt ctgcttgaaa gcaaggccaa gcaccaatat ctttttgcct
     4981 tccaggccgt tcaattcttt ttccagccta tccgtaattt ttccaacctg catctcattt
     5041 atctccgcag tagctctgag cagctttgga gcatagccga ggctttccgt aaaggctatc
     5101 aaagctctta aatccttagg aaaacagctc ccgccataac cgcgtccgga ttttaaatac
     5161 ttgactatct caggccttat aaattttccg tcaatttggg gactgattct tttgtcaagg
     5221 gtcactccat acagtacttc ttccgcatcc aatccgccga ttgcttctga tatcgccgca
     5281 atttcattgg aatatgaaat cagcattgca agcagtgaat tggaggcgta cttaataagc
     5341 tcggcggttc tcaggctgac cctgagaagc ggcgcctcga aataaccttc ataaaccatt
     5401 ttgaagtgct catagctcct gtcatcatat gcccctatca caattcggtc agggttcata
     5461 aaatccgtta cagcttcacc ttcccgcagg aactcagggt tcatgacaag cccgaattct
     5521 ccggccttct tccccgattc ctcttccagg atattcttta caagggtatc tgtggcagat
     5581 ggtattaccg tactttttat gcatataaca tgataggggt tttttatcct cagtattctg
     5641 cctatgtctc ttacagcctt ttctacgtta ttgaggtcta tactcccgtt cttgtatggg
     5701 gtatctacac aaacaatgga aatttcactg cttgccaccc catactcaat gtcagtggtg
     5761 gccagcagcc ttccttcagc aaccgcttca ccaatatatt cagaaagata gggctcgaat
     5821 ataactgcct ctgagttgtt aattctatct gcaatttctc tttgactgtc tacacatatt
     5881 acatcatggc ctttcttcgc caggcatgct ccgcatacaa gccctacata accggtacct
     5941 ataattgata ttttcatacc ccttctccta ttttcccata taaaaataat ccgtcacttt
     6001 cctgtcaata ccttctatct tctttatggc atctcttaat gccttgctgt atatcccgca
     6061 aacaacgcat gggatctcgg cgccgcataa tgcgacagta tctgcgtacc tcttcagcct
     6121 cagcccgctg aaatgataaa agatcagcct gcttccgttc acaagaatat tgccgtccat
     6181 ttcggaaaaa tcatatttga aataattcca ggaagccgca tttgctccgg gaatatcaat
     6241 atactcaact ccattaaaca ttccgctcat ggaatccaaa tagccctgat cggcaaatct
     6301 cccgttatca tatttgcaat agcaccattc aatacatctg tccttccacc atttcaatgc
     6361 cttatacgag tcattgcttc tcttgaaggc tgcaaagcct ccgttagtct ttctgttcac
     6421 cttatgggta gtcagaagaa catgccacct gtcttttctc ctgaaaagct tcattgggtt
     6481 atcaaagaaa aaaatatccg tatccgcata gacaaatatc ttgacatctg cgaacctttc
     6541 atacaaataa agcagcagca ccggcttcaa agtccagcag tattcgcttg ggcttctctg
     6601 acccttcaat tccctcagtt catcattttc ctgttcttca gcagatataa ccagcatacc
     6661 gggaagcttt aaagcatcaa ggaccctccc tgactcccgg tcaactgcaa gaactgctaa
     6721 aaatgcttca ggcatgcggc cctttaagga attgtacatg gctatgcatt gatggagcct
     6781 gtgttttgtt acgattgtac agaacattat attcaattta accacctaat ttattgctta
     6841 tggcttccca gttttcattc atatcctgca ggtctttgga agtgtccgcc gcctgccaga
     6901 agcctctatg ctcgaaaact gccagccggg attccgctgc aagcctgggc agaacgtcct
     6961 tctcaaatga acagccttca tggtcaagat aatcaaagat tgcttgcccc agaacaaaaa
     7021 aacctccgtt tatcagccct tctatttctg ttttttctcc aaaggatgct gcgagtccgt
     7081 cccgggcctt caaaattcca tattgactct ttttttctac agccgtcaat gtagctgtta
     7141 ctcccatttc ctgatgatat ttcagaagct tagcaatatc tgcatcacaa agcccgtctc
     7201 cgtaagtcat aaggaaagta tcttcatcaa taaaaggctg tatccttttt atgcggtctc
     7261 ccgtcatagc atctgcacct gtatctataa aggttatttt ccacttttca ggttttttca
     7321 acaggtacac tttgttttca ttgccgctga aatccaaaat gaaatcattc tgcatccaat
     7381 cataattcat gaagtactcc ttgatctttt ttcccttata ccccaagggc aatataaaat
     7441 cattaaagcc gtatctgctg tatattttca taatatgcca cagtataggg cggcctccga
     7501 taatggcaag aggcttcgga atatcctccg ttacttccct catcctcatc ccatacccgc
     7561 cgcaaagtat taccgtcttc aataacatca ccccggcaat ccaaaaatta ttatatgata
     7621 atcatcaaag cagccttaac ggataaatgt ccgtatcccc gggctcatat tgtcttgtgg
     7681 cggcagccag gataacaagc tcctcttcct catcattcct gaaacagtgg gctgtattct
     7741 cgggaacctt tattacaata ttgtcctttg acgatgctct gaatacatcg gtgctgtcgg
     7801 tttccaaatc cctgagggct atggtcgctg tcccttttat cacggaaaaa aattcaacgt
     7861 tcttcttatg gtaatgattg cccctgacgg aatttctctt tgtgtaaagc acgtagattt
     7921 cctcaacaga ctcatcagca ccaagcatat ccattgtgaa aacctttttc agttctcccc
     7981 tgccatcttt atagggactg attgtaataa cctcatacct gcttttcata aaaacacttc
     8041 cataatatct atatattact ttcctgctta taccattgat acaaccttcc aaggccggct
     8101 tccagctcca ccttggggta ataattgaga tagcttttgg ctttgctcaa atccgggcag
     8161 cgtctttggg gattatcagt caaataatcc ttttccttgc tttttttata ctcaatagcg
     8221 acatttccga ctatccctgc aatcagctct gcaagtccct tcatgttgat ttcggttctg
     8281 tcattcccaa tattgaaagc ctctccgtcg aactccgaca gtaatacaag catgaagcct
     8341 ttgactgcat cgcttatata gcagaagctc ctggtcgggg ttccgtcgct taatatctgt
     8401 atcttccgct tatggatggc gtcattgaaa aaatcgggta ttaccctttt gtcatacagc
     8461 ctcaggccgg ggccatatac attaaaaggc cttgccgact taatcggcac cttgtattcc
     8521 ttataatagt taacaaccat tgtttctcca agccttttgg attcatcata gcatgctctt
     8581 ggacccgtgc aggagacatt cccgttgtaa tcctccccgg taggtatgtg ggactcatca
     8641 ggatcaccat atatttcact gcttgaaagg ttcagaaagc tcttgatgtt ttttttcatt
     8701 cccagttcaa taaggttctt cagccccagt acatttgttt caagagtctc aatgggatac
     8761 ttcctgtaat aagacggcga agctatgctt gccgcattta ttatataatc aatgtcgtaa
     8821 tctatttcaa tcggctttga tatatctgca tttaaaaaca taaagttttc gttatgcctg
     8881 atatgtccta tcctttcagg cacaccggtt acaaaattat caacgcaaat agcggttaca
     8941 gattccataa aaatattatc gttgcagaat ttcagcaaat ccatgaaata agaacctatg
     9001 aacccgaccc ctccgctgat aagaaagctg cttccctcta taaccttcaa cgtccctccg
     9061 atttccagat atatatccct tacatcctcc tctattattt tatttatact caattttcat
     9121 ctccccttca cagaatactt gtcatggttt ttcctgaacc attctaccgt cctgtcaatt
     9181 ccttcctcaa ggttcaccct cggctcccag cccaatctgg ttctgatctt tgtcaaatca
     9241 ggctgagggc tccataaatc attttttcta tactcaactg ccccatagca tactcttttc
     9301 cccgtttcaa accttttcat aattagattt acataatgct tcagcttgaa aatactgccg
     9361 ctgccgatat tgaatatttc attcctgact tccttattga ccgcggccat cataaagccg
     9421 tcaattatgt tttcaatgta gcagtaatcc ctgtactgct cacagggaga cagggagact
     9481 tcaccatctt ccagcgcgga taatatcacg tggggaaaaa acttgtgact gccctctttt
     9541 tccccaaaaa ccccgaaggg tatgatagtg actatgtcaa ggttataccc ggctgctgcc
     9601 tggtgagcta ttatagtggc agcccctttt gtactgccgt atatgtttgt cgggctgtaa
     9661 tactcctctt ccttaattgc gcctttctta ttcccatatt gcatgcttgt tcctgtattc
     9721 agcaatttct cgcagcctgc cagccctgct gccatgacaa tatttaaagt acccattata
     9781 tttgtctctg cagccgccac ataatccttc tgccttgaat ccaccccata tgcagccatg
     9841 tgaaatacat attcgggttt tattcttgcc atacacagtc ttactgcctc gttgtccctt
     9901 atgtcaatgg caatcatcct tatggaatcc tttacctcgt ccagcctcca aagatcagaa
     9961 gcatccctgg ctgccgcaaa aacttcacag ccatacttaa ccagcctgct gactgtatga
    10021 gacccgataa acccattgct tcccgtaacc aacacctttt tattccttaa caacccggct
    10081 tccatactgc caccaccata tcaatccata ttttctatga ttgctgaatt tgatgccctg
    10141 taccaggcgt tcataccgga atatactttt gatatctcat taatatatgg ctttattttc
    10201 ttgattgact ccgcaactgc ccttgcataa ggaaagtata tgagttcctt tgcggagctg
    10261 cttaccttca ggctgtccca cttccacagg tcgaattcat tctcacccag aatcatgaag
    10321 ctgctgaaat gataaaatac cagaaggtct cctccgataa atatatcctc gcccctgcaa
    10381 tgcacttctt tatcttccag gttccacact gctgcattca taccgatgtt ttccgccagg
    10441 tatgcactcc catacggttt taagaaattt tcgagatatt tctgatcagc gaatctgccc
    10501 tccgaaacaa catcaaagca ccattcaatg cacttttttc gccaccactt cagatatgac
    10561 agcgctgtgc cgcacctctt gaaacctatt attccggcat tgaaccggcc cttctttcgg
    10621 tataaatgct tgtaaggctg tgaaaaatta tgatgggtca gaagcaccgt atgcctttcc
    10681 agggctttaa agcacctgtc aggccttgaa aacaagtata tatccgcatc taaatatagg
    10741 agtgaggcca cattttcata tttctcaaat atatgcagca taaacggcgc tttaagtgtc
    10801 cagcagtact cataatcctt cctgttcttc ttaactgctc tcagcttgtt gtcttctatc
    10861 tcagaaaccc tgaccaatgt cacattttcg agccccatac ctgtaagtat gtcataagcc
    10921 gcatcctcca tacagcatat ccatatatga aagctgtcgg tgttgctttc tatggaatcc
    10981 agcagtgcaa ggcatttcgg caggtattca agagttgtca atgtgcagaa gcaatactga
    11041 gaattgtgtt tatcctctgc agctcctgtt tcagaaattt gttccgtttc ctctgcattc
    11101 cctcgtttaa gctgcagcag ggcagcctcg caggcagata tataaggcat atatatgctt
    11161 cggacaaccg cttcggggaa cccccaatct ttgtaggtac aaaggtcata atcgtcagtg
    11221 cctgcatacc tgaagccata gtaatgaaac agcaccagcc ttcttgcatc tatatgcctc
    11281 ttctcttctg cggtaaacct ccatagtatg aagggcgtca tattgattcc ggcattcttt
    11341 atcacagcta ctcctttaaa tctttccggc cagtcattca catacatctg atcgctccac
    11401 agcccgttct ccatcctgtc atagcaccac tcattcactt tagactgaaa ccatttcagg
    11461 cattcaaagg aattcgcatc tctcctgaag cccaacaagc ctgtattgta tatgccgtat
    11521 atccggctca ggtaagcatg ctttccggtg tacctttcct ctgtaagaag tattgagtgc
    11581 ctgccccact ccaaatatat cgggccgggg tcggacagga acattgtatc cccgtcaagc
    11641 cacattatat ggtcaatgta attatagttg ttaaaaatat atagaaacgc cgagggcttt
    11701 acagtccatg catattcctt gtcattcctg tcttttcttg cttttgtaag ctccgggtaa
    11761 taagactcta ttgcactgac aggaataagc acagtatttt caagcttgag ggcagcgaat
    11821 gaagccaatg cttcatctcc catgcatata ataaaaaaat taaattcctt gtcatgttta
    11881 accattgaat tatataatgc aagcacctta tacttataat cactgcttgc tattgagcaa
    11941 tagtaatgga tcatcgatta cacggcccct cctaatcaat tcaaaaacgt ctttatttca
    12001 gaatatgctg tttatcgatg acaagttact taaaaaatat atttaagggc cagcatatgc
    12061 tagcccttta tcctgttatt caaaatattg ataagtttat tcaattcaat gcctctcgat
    12121 tcggcagcct gccgaagggt cctctcctga gatccccctc agccgcagcc tgcagacagc
    12181 agatcatagt atctcaatac acctttagtt cttggatagt atcttataat ctcttttatt
    12241 ttcatttcct cgcttatcat attgcattac gagataggat atgggggaat cggtactcca
    12301 agctgagggg gattgaatga agggatatcc tccatctcaa attcttcaca gaagtcaaca
    12361 tctatcggtg ctacatcctc ttctatctgg caatacccca ggacaggaac acaaagctgt
    12421 acttcagcgt caaactttac tgcaataaac gctcccaggt tggtaaccag tatctcaccg
    12481 gttgttgtat tgttctcctc atcaataact gcctcaccca ggcattgaag ctcaaatctc
    12541 ccttgatcaa ggaagtgcag cagtctttca aaattttctt ctgttacatt gggatagctt
    12601 cttctcacac tgaatttaaa gcagagttct ccgcagggcc cttctgaaag gttagttccc
    12661 tcaggcacat caagtacttc aagaggaata atgtttcctt tggcatcctt tccaaacaca
    12721 tcgcagcagc attttgccga gaatttaagc ctcttatctg aatttgttga aacatcctta
    12781 atttctacat tactcagctt gcagttcttc agtatcaaag caggctttat aatatctcca
    12841 tgtggatttc cgcagacttt gaacttgatg ccttctacac agtcccttaa aacaaattcg
    12901 tcaaatacct tttttacctt aaggcactca aaaaacaaat ctttacattc agtcgacccg
    12961 cctgttttta cctccagcct ggtggtccct gcacataata cagttggtaa tgaagcattt
    13021 ggcactgtca ttttctcacc ttcctataca tttacatttt ttcattgctg tctaatttat
    13081 aatatggaag tcaggacatt tttgtgaaca agttaaagca aatttaataa attttttaga
    13141 caaatgcaca atatacgaat aatgcgacgg ggacatgggg acggggacat ggggacgttt
    13201 cccctgtccc acttccagtg ggacacgcga aacgtcccca tgtccccgtc cccatgtccc
    13261 cgtcccccat gtccccgtcg cattattctt cagatgcgca agtccagtca tataagcata
    13321 aaaatatcca tataaatata gataaggaaa tttgaggtgg tgattatatg atagatataa
    13381 acccatcagc aatacttgag gattcaaagg gcaacaggta cttctttttt tacagggatt
    13441 cttcaatata ttacagggag gttcccatta agggggatat aaaggataca atgcttataa
    13501 gccaggcaaa cgcagatttt gctgctgtca tagatgcgga tgattccata tacctgacct
    13561 gcaacagccg atataaggga gtcctgcttt ttatatatac aaacagcggt tggaaatttg
    13621 aacctgtcgt aaatttgcac aacagctcca atatatttat tatggacatt atagtgcaaa
    13681 acggttccat acatatattc ttttcaaaaa agcttcctat tgccaatatg tacaatgttt
    13741 accacattaa caagaatata aacgataaga ccccttatat agagtattcc tggaggaaga
    13801 acagcctttc ggaaatatac tctcaaaaca ttgaaagctc ttactctctg ctccctttaa
    13861 gaaacggtat tattcactat gccagtgtat ggtacgatgg aaccctatat tatatcaatt
    13921 attactgcta cgacgattcc accaaatcct ggcttcacaa aagcctcagc atatcataca
    13981 aaaaccaggc gtttataaag ctcatacatc aaaataagaa aataaacctg ctctgctatt
    14041 caaatgaaaa cgagggaagc aacatacatc atttccttag taaatcttca agcagcagcg
    14101 aaatagactt cagggaactt ggaaatgtcc gtatcgatac caacggaatg ataccgctgt
    14161 tttattccga tgataaagca atacagatgg cctggataaa ggatcatgtt ttccatcaat
    14221 acacctttga cgattcagcg ggcagatgga gaaaggctgt tgacttgccc gttacggcgg
    14281 ataccagtat taatgcagta aaaataatga aaagcctggc cccgctgtca ataacaaagg
    14341 gctttttcat acttgacaaa aactacaaca tttcaaggcc tgcagagcat atatcaagga
    14401 gtattcctga ggataaaccg aaggaaaaag ctcaggctgc ttccattcct gatatgaacg
    14461 attatttaag acagattctt gatgaaataa gagacctctc ggataatgtg aagtacctga
    14521 acagcaggat aggaagcagt gataacagga attcaaaaga tcccaccccc ccaaagtcag
    14581 gtactgttga taaaactgca acggaacagc cgacccttaa gaaaagcggc ttcaaggaaa
    14641 agttcatgaa aaatgaaagg cttcccaact acgaaagctt attgatgaag caggagaata
    14701 taaccaccta cgtaggaaag cccggcaccg tcaaccccgc ttccgatatt tcaaaagaca
    14761 gcgagaaaca agccttcgct tcccccaata cggcagcacc aaataataaa gaaaactgga
    14821 cggaggatcc ggaagagatc ccatataaat ataacagcct tatgaaaaaa ataggagaat
    14881 tctttaaata gaattctcct tactgtcata tttcgtactc cccggtgaat acttcttttg
    14941 cttcaccggt catttgcacg tgttccccgg ccttccattc aacagtcaaa tccccgcctc
    15001 gaagatgcac cctgacaata ctcccaagct tcccgcttgc aattcctgca acaaccgaag
    15061 cacaggcacc cgtgccgcat gccattgtat agcctgctcc tctttcccat gtacgaagga
    15121 ttatttcatt cctgtttatt attgaaacaa aattcacatt tgtatttcgt gggaaataat
    15181 cagatacttc aatttttctt ccttccgaaa tcaccttttc atcctcgata ctgtccgtaa
    15241 aaacaactgt gtgaggaaca cccataaaca ttgaagtaat gagatattct tttccgtcaa
    15301 cccggatggg ataatcaact actttatcca agtcagcatt aaacgggata tccttgctgc
    15361 caaacttcgg cattcccata tccacggtta cctctttcac tgctccgtcc tcaatattca
    15421 gcctgggtga tatggtaccg gccaatgtct ccacagtaaa gctctccttg tcaaccagtt
    15481 tcttctcata gcagaacttt gcaaagcatc gtgaagcatt tccgcacatt tctgccatgc
    15541 ttccgtcaga attgaatatt tccattctga tatcgcagct ttcacttggg gatgcaagta
    15601 caataccgtc cgcgcctatg ccgaagtacc tgtcgcacat tttcctggca aggctgccgt
    15661 caatatggat attgcttctc aaattattaa tcagtataaa atcatttccc agtcccgtgt
    15721 atttgtaaaa cttcattata ttgcctccga acccggtacg gcatcactta aaattaccgc
    15781 acttattctg taaattgccc ttgagcctct cctttaatta ccttagccaa ccactctttt
    15841 ccctcaacga gcctcgttac catcattgca gctacactgt ctccgcatgc gttaaccatt
    15901 gtagcgggag ggtcaaccaa aaatccgatt gtggcaataa tcggaaaagc ttcaggcgga
    15961 aaaccataaa gatttactat aagcatttct ccgatcaatc cgcctccggg tacccccgac
    16021 attacaacac cgctcattac tgatatcaat attgcagttg caaaggtccc tattcccgtg
    16081 aattcttttc cgtaaagtcc gaacaaaaag gatatcttca aaattgccga taagcacgaa
    16141 ccgtccatat gcattgtagc acccatcgga aggactattt ccctgatatc cttcggaaca
    16201 cccatttttc ttgctgattc aagattaacc ggtaaggttg caatgctgga ttgggtcgct
    16261 aaagaggtaa atgcaggtga tacaatattt ttgaagaatc tctttaccgc cggcattcct
    16321 cccgaataat atgcgtatac gaagaaagct ataaagaaat aagctattgt aacaggataa
    16381 tatatagcca tagccctggc gtagtccttt agaagctccg gcccgaactc ccctacaaga
    16441 gccgcaaaat atgcagccag tccaatagga gcatagtaca ttataaagct taccattttc
    16501 atcatgacat ttgaaagtgt atccaatgcc tccgcaacct tcttgccttt ttcacccaag
    16561 gcagttacgc agaacccgaa cagcactgaa aatactataa gcggcaacat attccttctt
    16621 gataaaagct ccggaaagtc tgataccgtg atagccttta ctgcttggtt tgcaaggctg
    16681 atcttctcta ctggctcctc agcattgacg tttagattaa ctccttgtgc aggtggtata
    16741 actgtcacga aaaatatcat tataatagct gaaattatac cggtaacaac aaataccaaa
    16801 agcagattct tcaaaatgct gcccaatctt ctcatatttg ccatatttgc tactgcactg
    16861 gatattgtaa taaaaacaag gggtacaaca acagtgaaca tgagattcag gaaaatatcc
    16921 cctaatggct tgaaaactgc agcatccgcg cccatagcaa tacctatgat actgccaata
    16981 attattgcgg taaacagaat tatcgggaaa cgatatgcct cccaaaccgt ttgtgccctc
    17041 gtactcacac aaatcccctc tttcttataa tattttatga aattttatgc atttaacact
    17101 cattcctcaa caggtcctca taggtctgcc gctttacaat aagccttggc tttcctttgc
    17161 tcagcataac aacggccggt ttgggattcc tgttatagtt gcttgccata gagtagccgt
    17221 aagcacctgt tgatttaacg gcaagaatat ctcccggctg aagctcggga acatataaat
    17281 cccatataag tatatctccg ctttcacagc atttgcctgc aattgtaaca cgctctttct
    17341 tgggggaatc tgctttattt gctatgattc catcgtattt agcctgataa agtgccggtc
    17401 tcgggttatc cgggaatccc ccgtctatcc ccgcataagt tctgaccccc ggtatttttt
    17461 taatgctgcc tattgtgtaa agagtaatac ctgcttctcc gactatccag cgtccgggct
    17521 caataaaaat tctcggcctt tttataccgt tattactgca gaactcctca atactattca
    17581 tcataggatc aacaaaatat gacagcttcc ttttttccgt atcgtccaga tactttatcc
    17641 caaagcctcc tcccagattg aattcttgtg caacataccc gacttcatct tttatcttct
    17701 ttatgaggtt cagcataata ccaacagcca tgatatatga ctcattctcc agaatctgtg
    17761 acccaatatg gaagtgcagc ccttttaagg ttataccttc tgattccagt gaaagcttta
    17821 cggcttcgtt tacatgaggt atcggaattc cgaactttga gtcaatattg cctgtcaata
    17881 tatacttatg agtatgggaa tccactcccg gagtaatacg taaaagtata tccgccttta
    17941 cgcccatttc ctttgcaagc ctgtcaatca actgaatctc ccagatactg tcacatacaa
    18001 aacgtccgat tttgcattca agtgccgttt tgatttcttc gggagttttg ttgtttccgt
    18061 gaaacattat tttctccatt ggaaaatcca ctgattttgc cgtatacagt tctcctcccg
    18121 aaacaacgtc cagtcccatg ttttctctct tgattatcct tgccatttcc ctggtcaaaa
    18181 atgcctttga tgcataatac gcttgtacgt cctgatactt ttcagtaaaa gaatacctga
    18241 tttcattcag tctctccatt ataatatcct cagacattac ataaagagga gtgccatatt
    18301 cttttgccaa tgctacagta tcacaatccg aaaaatagta gttcattact aacaccttct
    18361 tttataaaag ttgctgcata taaaagtttg atcccgatta gcatgaactg aaaaattgtg
    18421 acctatgcac ggcttcctgt taagatcatg atttaaaatc attttaattc ttcgattgca
    18481 taaaaacaat atccaaaagt gttatcttaa cagaagcact ttttgccaca actctacatt
    18541 ttaggataga catcagctaa taatatttcg gatatcctaa aatgttttcc ttgattcata
    18601 gaatacgcca aaggatgtac attattcctg attagctgta tactggcgta ttctatatat
    18661 catttcagac atgcctgatt atctgatcct tctattttta agcttgggat cttttatttt
    18721 actttcatat ccgttgtctg acggaaaata gtattgcgcg tctttgattt catcgggcaa
    18781 atactgctgt tccacaaaat tgccctcata atcgtggggg tacttgtact caaccccgta
    18841 tccccaatcc cttgcacctt tgaatgcatc atttctcagg tgcagaggca cagccccggt
    18901 tcttttgttt tccacatcct tcattgcctt tccgattcca accactgcgg aattgctttt
    18961 cggtgcgcaa gccacgtaca aagctgcctg ggacaaaata atctgtgctt ccggcatacc
    19021 aatcatatga actgcattgg cagccgccgt tgctactatt atcgcgtttg gatcagcatt
    19081 tcccacatct tcggaggcac aaatcattat ccttcttgca atgaacttgg gatcctcacc
    19141 tgcataaagc attttggcaa gataatgaag tacggcgtca ggatcagaac ctctcatgct
    19201 ttttataaat gccgatatgg tatcgtaatg gttctcaccc ttcttttcat aatttacggc
    19261 tcttttctgt atgcagtcct ccgctgtctt caagtctata tgaatcttgc cgtcactgtc
    19321 cctttcagtg gtaagggccg ccagttccac cgcattcaaa gcagccctgg catcaccgtt
    19381 ggctatattg actatatgag ccattgcttc ctcggttatc tcaatattat aagagccaag
    19441 ccctttctct tcatcagtca ggcatctttc aaccagtttt ctgatatcct tagctttaag
    19501 aggattgagc ctgaatatag tggaccgcga aataagcgca tcattcacct cataatacgg
    19561 attctctgtt gtagctccta ttaatatgac agtaccgtcc tcaacatacg gaagcattgc
    19621 atcctgttgg gctttgttga aacggtgaat ctcatcaata aatagtattg tcctttgacc
    19681 atacatcccc ttgcggtttt ttgcctcctc aactaccctt cgtatgtctc ccactcccga
    19741 agtcaccgca ttaagctgct caaaatgaga tttgcttgta tttgctatta tctttgccaa
    19801 ggtagttttt cctgttcccg gcggcccgaa aaaaataaga gaagtgagct tgtcagcctt
    19861 tatagcacga taaagcagct tccccggagc cacaatatcc tcctgaccga caaattcgga
    19921 aagatcactc ggcctcatcc taagagcaag agggctctct ttcttcatct cctgctccct
    19981 gttcatatca aaaaaactgt acatatcttt cttcataagt atttacctgc tttctcaaca
    20041 caaccattct tatggaaaag gcaatgggct attcttgctc ccatcgccta ttgaatcaca
    20101 tttgccatta atccctgaac cgactagaat tgtactttca cattctgtct ttctgattca
    20161 tcagctttga aatagggttc ctccctgaag ccgcacaagc cggcaatgat atttgccggt
    20221 acaacctgta tagcgttatt gtacataaga accgtatcat tatagaactg ccttgcataa
    20281 gcaatcttat cctccagctc ggacagctcc ccctgaagtt ccatgaagtt tgtattggcc
    20341 ttcagatccg gatagctttc tgccactgca aacaatcggc ccaatgcccc tgtcatttcc
    20401 gtgtttgctt tcatcatttc ttccggggta cctgcgctta tgtactggtt ccttgctttt
    20461 acaacagcct ccaaggtctc tgattcatgt tttgcatagc cctttaccgt ctcaacaagg
    20521 tttggtacga ggtcaaacct tttcttcaat tgaacatcta tctgagccca tgaatttttt
    20581 accctgttcc tttttactac caaactgttg tatgtgctga ttgcaattaa aacaagcagc
    20641 agcagtacac caaatattaa tattttcata aaatttcatc ctccttttaa actttctgaa
    20701 acgaaaaacc acggcttaga atgcgccggc tccgccaccg ccgccgcctc ctcctccgcc
    20761 ggaaaagccg ccgcctcctc cgctgccgga ggaagccttt gacattgctt gcctgtagac
    20821 ggattctgta gtcttaacca ttgagtcagt tatattatct attgtatcaa agtaattgtg
    20881 aatgtgtcca tagcgtccgt agtacaaata tgtcaaatgg ctgttattaa agtcttcctc
    20941 tctgaatacc agcctgagct ggcttatgac ctctttggca actccaaggg atatggcata
    21001 cacaaggtaa tgctcccaca tgattactgc aggcaaatcc gccttatcaa ggctgctgaa
    21061 gtgcctgagg aattttctga aagctttcca catcttgaac tgctctacgc cggatttcga
    21121 ccttctcctt attgtcaaag agtatatcag cagtataatc gataccagca tcaatattgc
    21181 aaatccaaga atattatcat gtcttgccgc agtatatccg ccgaaaatca taccgataac
    21241 agctgttaaa acaccgaaga gtatgccttt aactgtgttt ttgtcaaaaa aggaataact
    21301 gtctgcttcg gattttacaa gtccggtcca aatatcgtaa tctctgctga aatctattgc
    21361 actttctcgt gtcttagaag aattctcaat atcctcaaga gatacccgtt ctccgtcccc
    21421 tattctaaat atcagccatt ctatggcata tttctcatga ggggaaagaa ccatcaaatc
    21481 agcttccttg tttaacttga atatatactc gttatcagct ctgctgccga agagtccgtt
    21541 aacctcttcc ttttcaacaa taagctccag gtacttcatt ctgaccaagt ccattaatgt
    21601 agcggtcaag tctcttggct tcacactgcc aaagttccac agcactgcca taactgcggg
    21661 ggaatagctc ccgggaagct ccctgaaata atctcccgcg aattttgcct tgtattcctt
    21721 atcaaatttg aaatacaaat aaacaatcat caatagctca aacaatacat atacaaagga
    21781 aaacccgaca attatccttg cctttgtcct tatctcattt gcctcatcgg caaattcctt
    21841 ttcctcagac attatctccg caagggcatc cctattaaat accttttttg aatctttaat
    21901 caattcaggc ggaaaaagta ctcttgcttc gacaaagttt cgagccggca attcatccac
    21961 tctcaaaact acggtttttg aatccgcgat ttcggaaaaa ccggataatg gcccatgccc
    22021 gaatactttt atctgctctt tgtcagcacc ttccggaatt ttaattacaa gctcaaagtt
    22081 ctctatttta acttccgtat cttcgcccat gaatttccaa tagatttccg caatatcatt
    22141 aaactttgcg gcagcgttta aaactctgta ttttaatata aatgtcctgc tttcatttac
    22201 agcagcagag aatatcttaa gctttatgcc gccgtcttca ccaatcagtt gaaatgtatt
    22261 atccctttcc gagttatcct gtacaaacgg agaaagcata tcaggctgct tcttgtatac
    22321 ctctacagcc tctataccat cgctgcctgc tgtccggagg gttctgtaca cgcccctgaa
    22381 gcttccgtca aactcataag tgatttcttc aatgatatcc atattcccca tactgtccaa
    22441 ctgggcattt atcttgaagc tgctcatata atagcttctg tcgtcacaat atgcagttat
    22501 cacggataaa agcgcacatg tgatgagaaa tatgagtaat atggtaattc tccttctcaa
    22561 atccgacctc acctcctaga caacattgta taacaattag tggaaaagta aaagaaaaaa
    22621 gatgaaggac acggggacgt ttctcctgtc ccgctttcag cgggacagga gaaacgtccc
    22681 cgtgtccttt atggcgaccc tgctattcta ttatcttttc cacatattcc gaagtaaagg
    22741 ctgcaagcaa tgcgccgtag cccagatcaa actccatgat gtctcctacc ttgtattccc
    22801 tgccgcagtc acttacatca attattaaat ggtcgctgct tgctcctatt actatggcat
    22861 ccttgtctct gggctgtatg ccgtccgcct ttaagtcctg cctgcctgaa gccaggatag
    22921 ctcttttcct tatgcctctg tcgacaaaca caggtttttc gccgaaggcg tcaaccccgg
    22981 tctctccaac aggtacggag ggcttttcct ttagctctat tatttccgcc atgtatgtga
    23041 acacatctcc gtaggtgcct tctattcttt ccccgtaagc agtctcattt ccaagaacaa
    23101 ttgcttctcc aaggcgcagc tggtttatac ctttggggac ttcattcttt tccaccaggt
    23161 gcaagctgct tgaattcccg ccggagatta tatccagctt aatatcatat ttcttttcaa
    23221 tctcttctgc cagggctgcc agcagcccga ggttctccac gcttgggatt actgctccgt
    23281 agcaggtgag gtttgtgccc agccccacaa gctttatacc cggcagtttc actattctgc
    23341 cggcaaattt aatcgcatct ttataccata cgccttccct gaggtctcca aggtctgcca
    23401 taagtatgat tttatgaacc ttgccccttt tctgtgccct atcggaaagg gcttttataa
    23461 cctcatactc tgagttaagg ctgatatcaa catgttcaac tatttcgtca acctgtgaca
    23521 gcatcggaat tctgagaagc attttttccg cattcagctc tttcagcttt ttaatgttct
    23581 caatccttga atcggcaagc atttttgccc ctgcagccac ggatgcttcg gcaatctccg
    23641 gcataccgca gtataccttg gttacgggta ctatgtcaat cccaagcctt ccgcactttt
    23701 ctgccagtac tttggtattg tgcttaagct tttctttgtt gacttttatt gcaggataac
    23761 tcatttttca attcaccccc aaactttcct tcataagtgc cagtgccgct tcgggaaacc
    23821 tctttgaaag cactcccagg gatgccatta tgtaatggtt gtcaatcagc acctttgctt
    23881 cctttcccgg gtacaattca gtccctttgc cctgcagccc cacggatttc aatatgtcaa
    23941 tacgcccggg gagcctggtc agcgcccctc ccgttccgat tatatattta acatttgtca
    24001 ggtcctttcc ttctgcaact gtcttctttc ctgtcgggcc gtaaaggaac cgcagttttc
    24061 ctgcatgcct ctcaagggat gtaagaacag cttccttggt cagtctttct acaaaagcct
    24121 tttcccgttc ggtttcggga atcggttttg cattatttac aagctcttct gcctccggaa
    24181 attccttctt aagattttcc aatccaatct tttcaactat atgcggaata ttcacatgta
    24241 ctcccaaatc tccctcaaca gtcctctttg ccgcaggttc agggcttata agcataaggc
    24301 ttatttcttc ggagccctct gtgactgaat gaacatccgt agtggcgcct cccacatcga
    24361 aaaccacaag gtcgcccaaa tgttccttca atagcctggc agcctccatt accgctcccg
    24421 gcgttggaat gatggggcct ctgaccatat ctctcacagt gctcattccg ggagcatgta
    24481 ttatgtgctc ttcaaatacc tcctgaatga ctttccttgt tggttctaca ttaagcatat
    24541 caaccttcgg atatacgttc tccactatat aaagccttgt atttgtctct tccgcaataa
    24601 gccttacttc ctcctgattc tcgatattcc cggcatatat taccggaatt cctaatccca
    24661 tggaagcaat catttcaaaa ttgtgcaggg cggtatcacg ctctccgtaa tccacccctc
    24721 ccgcaaccag tattatattc ggccttacat cttccagcct cttcaaatcc gttctcctga
    24781 gcctcccggc agtaacatgc tttattattg ctcctgcacc cagggcggct tcctttgcag
    24841 ctctgaccgt catatcgtaa acaagtccgt gaaccgtcat tctgaggccg ccggcagcac
    24901 tgctggttgc aaggaattca ttccattcaa gctcaggtgc tccaaggttc ctcttaaggt
    24961 catccactgc acctttaagc cctatagtaa catcaccatt atacaccgaa gtaggagctt
    25021 gcccttgtcc gaggaattta ggacaagggc tcataatatt gtcaaatgca ttgaccactg
    25081 tagttgtact gcctatctct gcaacaagca cactaatttt cattgctgtg cttttccctt
    25141 cttctcttga caaggtagct tgctacatca attccgtggg tacctctgcc gaagcccgca
    25201 tccattcccg cttcaacagc tatttcattt gtgacctgtg ttccgcccac aacaagcatc
    25261 actttatctc ttataccctt ttcaacacaa agatcgtgaa tctttcgcat attctttgta
    25321 tggacatcgt catgggtaat tatcgtgctg gcgagtattg catccgcatt tgtctctata
    25381 gctgcatcta caagtttttc aacagggcag gaggttccaa ggtacaggta cttgatacca
    25441 aaaccttcta tccctccatg ctttatatcc agagtctcct tcatccctac agaatgctca
    25501 tcattcccta ttgttgcagc tactacaaac attggttttg ctgagatatc ttcccttatt
    25561 tcctcttctg ataatgtttg aaccttttca ggtattacaa gctcattaac attaatgtca
    25621 aatataactt ttcctttaat ttcaactaag gtaccttccg acggatgcag cgcctgcttg
    25681 tgcactacag tgacatcttc aaggcccagt ttctctccgc attttattgc tgcaaattct
    25741 gcagttcttt catccagtgg gaggaatatg gtagttgcta caacaccgtc agctctccat
    25801 tcaacctcag gttttacagt attcgtatta taatattttt ccagatcagc aagccttgta
    25861 ttcacattat catactcatc aagctcatct atatagataa tcttcgaagg atcttcaaag
    25921 gtatcattgc ctatggcatc tgatgccttc ttaaattccg aaggtatgtt attatatccg
    25981 tagtgaacag atacaggagc aaagtagtcc ttatcccttt caaatatcat tcccacacct
    26041 attccgccat caatctgtct gggaataccg tcaccatttc tttccggata atatccgctg
    26101 tcgatgaaga atccattttc cacagcctta aaatatccgc ctgtttccaa tatctcttcc
    26161 atgaagagga ctgctctttc tttaagctcc cttacttcct ttcccaaagg gccttccctg
    26221 tccaatttaa ccatctctcg aaggccatcc attccattaa gagcttgttt agctgtattg
    26281 accgcagcta tgttattgta atgccacggt acattccttc cttcatcggg agtgatggtt
    26341 gactgtatat cggcacttgt aagtcttgaa atcaataaat caagagtatg tgtaaccgtt
    26401 gcttctctca tgtctgattc catatatttt gtgttctgct gagctctcat tttgtactct
    26461 gagaagaagt cccttagtgc aaccgcataa ggcaggtcaa tcctcatacg cggcgccggc
    26521 ggcgccgtgg gcggcactgt tgatagggct atgttttctg gtttaatacc aaccttccgt
    26581 gagaatatac tgtttatggc atgctgaacc ataagctcag gcctgacctt ccatgccttc
    26641 attgcagtag cattggcatt atgggccccg tctatctgca gaatatccgc ccatgccatg
    26701 accttcttcg cctcgcaggc atctacaaag cttcttacca tgtttacatt tctgtacaat
    26761 acgttgtatt gaggatcctg atgagcgcca tttactcctt cttccgcgaa cataacagca
    26821 atatcaggtc ccgcgacacc ggatatatat gaatggaaat taatcggcct tcctacttca
    26881 tcctctatca tatcgagagc ttttcttgtt gccctgcact gctttcttgt aacggctata
    26941 ccacctattc cctgtgtagt tccctcaatc aatgagtcaa tatgagattg ccccattgtt
    27001 ctgataacca taatatggtc agccccatgc catgccgcca ttctcattct tctgatatca
    27061 tcttcaaatc ttccggaagc aatttctgta gtaactacac aatccggctg cggatcaatg
    27121 taattgaatc ctcggcttcc cggcaaggga atgtagttct ttagaccttc cgaggtttca
    27181 tggtattcaa aatcccctat cttcctattt ttatttctgc cttctctcca gtgccagcct
    27241 cttcttctcg gtctgtagct ttcaaggtcc tgcaataatt cctttatatc cagcttttta
    27301 ttcggatcca gtttcatttg ctccccctcc tttaaaacaa tgcgtcgacg tcgtcccagt
    27361 attttccttc tgcaagtccg actcctgctt ctctcacaga gacccccagc ctctttgaaa
    27421 gcctccatac tacattaccg gcacccttgc ccataagtcc ttttctcatt acgccttcaa
    27481 ctataggctt tacttcaaga cttgagaagc ccatcctgag aagtatgctt ctttctattg
    27541 cgggagttgt gttcttataa ccaagagcca gcatcggatc aactattttc tcagccagct
    27601 cccagaacct ctttttcagc tcttcgtcag aaagctctgc caggtgcttt cttcttattt
    27661 catagtcatc tgcacgtttc actgccattt ctatccctcc atctacttta ttttcacgtt
    27721 tagttcagca agtacgcctt ccacaaaact gtcatcagtt tttgcatccc ttacaaggaa
    27781 ttcaaaatcc tcttttgtaa tttcagttac cttgcaatta ttgatgcagt ttttgatata
    27841 tgatgctctt gccttgtcta tatcgtaatc tcttactttt atatctgacg gatgtgccgg
    27901 aagtataata ttcttcccgg gttcgtcacg gtcgggattg ccgaacaata tttctatacc
    27961 gttttgcttt gcaaatgtaa gctgcggcag cggatgcttc cctgctcccg tatattctgt
    28021 ttcctgaacc acaataatct ggtccttatc catttcttgc gccagggcaa aagcagctgt
    28081 taaggatgtg tttccggcag gccctctctc cagtccttca agcactgata atgcttctgt
    28141 aatatagaat acttcacctt gttttaccag aacatatctg tcaagatatc ttaaaggcct
    28201 tgcagcgttt ctcggtacat ctgccctgtc aggccatgtg gcaaagggta ttccgaagcc
    28261 tgtgtggcct gtagtaaagg attttctatt gaaatccctg tcggaggcca tatgaagacc
    28321 cgaaaggtca acgcttgcac cgacaacctc aacatccttt gctcctgctt tcaaaagccc
    28381 tcttgctgta cctgttacat ttccgccccc tgcgtgtgta attacaacct tgtcgggatc
    28441 tcttccaatc ttctccctga actgcatggc aagctcatag cccagtgtct ctacccctgc
    28501 tattccaaaa ggtgtatata gggatgcatt aaagtagcct gtctcctcca gcaaggtcag
    28561 gaaggtatag aagagctccg gtcctactgt cagctgtacc acctctgctc cataggcttc
    28621 acaggcacgc tgcttttcaa gtatttcggg ctgtcccacc atcctgctgt cgtaacattc
    28681 ctgaacaaca atgcatttaa gccccctctt tgcagcctgt gatgccaccg ctgcaccata
    28741 gttgccgctt gttgccgtta ccactccttt gtatccgtgc ttttttgcat gatatatagc
    28801 agtagccgcc cttctggcct taaagcttcc ggacgggttt gcagcctcat cctttataaa
    28861 aatccttgcg ccttttccct ttggagcaag ttttcgggcc aatgcagtaa gatttttcag
    28921 ctcaacaaca ggtgtgttcc ctacgcttgt ctcagcctgt atctgctgca tttcctccag
    28981 cgtataacct gcctctctca tcattctttc gtaatcgaag gatattcctc ctgattcaaa
    29041 tacgctatag tcaataccaa ttgcctgttt cattatttca ttttttcttg ccataaccgc
    29101 tgcgtatgac atatccttac tcatttgaat cacctccgaa gaggatttcc ttaagctgca
    29161 tatccacctt taaaagctca ggtacgaaat cgccatagtc atgtatatac ctgggattaa
    29221 ctgcggtcag ctcgcctctg accttcctgc cggtggcagt tacgatttca accgtcgaac
    29281 ctatttttgc atcctcttgt gctataccct ttacccataa ctcaagaggc atcttcttgg
    29341 tgtcctccgg aacctgcggc gccctttctt caggtttcag aacaacctgg cgtatctgca
    29401 cccagtctcc tttttttgcc gagatcattt aacaacactc ctctttatca ctattaaaaa
    29461 tgcgtctcac gacgcgtttt tttttgctag ggttttaaat aagtaccctt cgtttttgct
    29521 ccgcatcctg aaactccttt atcggcaggg gcttcttccc cttgcatccc ccattgtata
    29581 ggaaagtata ctttcctata caataaaaaa tcttttcgcc ccgtaataag gcaaaaggga
    29641 catatttgta ttttaataat aaatatatgt cccttgtttt aactgctatt cttctatcat
    29701 ttctctcata tctcccataa tagctctggg cacagggagg tcaatcatgg tcttaagacc
    29761 ggcccttgca ttgatgacat gaggaatcat attgacacac attgcgattg taccgatacc
    29821 tccgggtatt tccggcttgt ttgccatatt tacatttggt gtgcctttga taataatata
    29881 atctccggtt tcaactcctc caagatgagg ttcaatctgc tgcggatgat ccatttcaat
    29941 tttaagctct ccgtcaacgt atccatagcc cttcatggca cagcccgcaa catctcccgc
    30001 cttggcaaat ccataaggtg acttcctgtc aacttcggtt acaatcggct ccatgctctg
    30061 gttgaagtca ctgagcttcc agccaatggc atccgctatc atacctatgg attctgcaaa
    30121 acctacatgg cctgccagct ttcctgtcct gactccttcc ttgaattcat caactgtaat
    30181 accaattccc tgctcttcca ttactgccgg cccgaaagga gacaagctgt ttacacgtct
    30241 tgcaacaata tgctctactt cttcacaggc acctgtaaat gcaacaacaa gaagatccat
    30301 aataagacct ggatttatgc ctgttcccag cacagtaacg ccattagcct ttgctatctc
    30361 gtccagttgt tttgtaagtt ccggttcagc agcttttgga tatgccattt gctctgcact
    30421 ggatattaca tttatctttt tctccagaca gtactttatt ttgtcaaaaa cacctttagt
    30481 aaatgaatcc gtacaaatca gcacaacatc tgcagcattc tctttaataa catcctcata
    30541 ggatccgatt attacgtcct tattgctgcc tctttcagtc tttatgtaat catacatact
    30601 tttaccgatt tttgctcctc ttccaacggc accaactatt tccacgccct tctttttgag
    30661 aagcatatca gccattccgc cgcccattgc acctaatccc cagataatta cctttacatt
    30721 ctcttttctc attaagcaaa cctcctaata ataattagag cactgcaaat tattgtaagc
    30781 aaacctcatg ccaaatgagc cattaagaca gtagataaaa aaatattttt aagtgttgat
    30841 attactgcct aattgatgcc ttaccactta attgcatatt tcagaattat gaaacatacc
    30901 atatccgcgc aaatttattt gcatctctgc caatttcttt gcactccgat tgagtttttg
    30961 ggaaggctat tccttcaatc tgcttgtttt aaaaccaata atttagatat atgcaaattt
    31021 ttttgcatta atattgcatg tattctcatg gcttgcattg actttcatta ttataaacct
    31081 taaaatggat tatatgctct acgataaata aacgcacttt ataaggagat attgccatgt
    31141 atgattttca cgtacattct cacttttcct ctgattcaac cgcatccatg caaagcatgg
    31201 cagcgaaggc aaaatgcata ggccttaagg gcatctgctt tacagaccat tatgatttgg
    31261 aatatcaaag caccgaaatc agctttgaat tttcctatga tagatatttt gatgagatta
    31321 acagcctcaa ggcaaattcc tctgaaaatc ttgaaatata caccggactt gagttgggga
    31381 tgcagcctca cctgagtgct gagaatgaag ctcttcttaa aggcaggagc tttgatttta
    31441 taataggttc catccattgt gtgaataaga aggacatata ctgcaggact tttcttgaag
    31501 gcattactga aaatgaagga ataagaaact actttgatga tatgctgcag tgtattgagg
    31561 aattcaagaa ttttgacatt ctggggcatt tggatgcagt aagaagatac ctttacaata
    31621 aggataacag tttctcattg gacctgtatg aagaatccgt caggaatgtg ctgtccaagc
    31681 ttgtgcattc aaacaaagga atagaagtaa atactgcagg ccttcgttac tgcctctctt
    31741 cctttcatcc gctgccggat attctcaagc tgtaccgttt gctgggaggc gaaatagtga
    31801 ccgtaggttc cgatgctcac aaacctgatg acttgggcta tggaataaaa aatgcattat
    31861 ccgtcctttc ggaacttgga ttcaaatatt atgcaatata taaggacaga aagcccatat
    31921 ttataaagat ataggaggca gaagcctcct atcactatca gtttgcagac aaagttcatg
    31981 taaagcagta gggacggttc ttttttaaag cagtagggac ggttcttttt gcttcaaatg
    32041 caaagcaaaa agaaccgtcc ctactgcttc aaagcaaaaa gaaccgtccc tactgctttg
    32101 cctctctttc gtaccttttt gcctcctatt cctttatatt aatatcatat ttcttcagct
    32161 tgtgctgaag ggtttggcgc tttagtccaa gcatttcggc gcttctggat atattgtttt
    32221 cacacttttg cagtgcctcc agaatcaacc ccttttccac ctctgcaact atcctgtcca
    32281 ggggctggtc ttcattcaaa ttcaggtaat ggttgttttt gccggccttg aacattactt
    32341 cacacatttc ctgggagata tgttccggct tgattacgtg ccctctgttg accatattta
    32401 ttgcactttc aattatattc tccaattccc tgacattgcc cggccaatta taattcagga
    32461 aggcctcctt tacctcattg gaaacatacc agatatcctt ctgaagtata ttgttatact
    32521 tcttgataaa atggtctgtc agaagcataa tatctgtttt tctttccctc aggggaggta
    32581 tgctgacaga tatgacatta agccgataat acaaatcctt cctgagaatt cctttttgaa
    32641 gcagtaccaa agggtcctca ttggtagttg ccagtatcct tacatcaacc ggaatatccg
    32701 aaagcccccc tattctccta atgttgccct cctgcagcac ccttaaaagc ttcgcttgaa
    32761 gctgatggcc catggaattt atttcatcca gcatgagggt tcctccattt gcctgttcaa
    32821 acagtcccgg cctgttgata gctcctgtaa agcttccttt aactgttccg aacagtattc
    32881 cctccagaag cgactccggc agtgcagcac agttttgtgc tatgagcggc ttatgcttcc
    32941 tgctgctttc attatgtatg ctctgggcga atagttcctt tccggtaccc gtctccccgt
    33001 atattaagac tgtggatgat gacatagctg ttttcctggc tatttccacg gcattaagga
    33061 atttttcgtc ttccccgatt aaatttctga aggtataccc ttttaggctg ctgccatcaa
    33121 tatctttgct tgcaagctcc tgatgcaggt atacaagctt atcggacagt tccttctctt
    33181 ttgttatgtt ctttgcaatt tcaagggctc cgatcttgcc ctgagatatc ctgataggta
    33241 tagtgctgtt taaagtgctt atctgcttgc ctttattgtt cagatatgtc tgatatttgt
    33301 tgtatattgg ctttcccatt ttcagcactg tcagaagcgt actggtctca gtggtcaggc
    33361 tggggaaaat gtccagtatg cttttgccca tgacctcatt tatatccagg ccttccatat
    33421 cggccatagc ctgattgtaa ataattgtct tccctgagct gtcaaccaca tgaataccga
    33481 tatctatgtt ttttagtatt gtatcaataa ttgacttata aattaaactc atcgtcatat
    33541 cattacctca catgcaaata caataaaaag gtatgccaat acataatata tattggtcat
    33601 accttattat atcataaaat ttgcatgaat ttatggttta ttcaaactcc ggataaagag
    33661 gatgcttgcc gcatagcgcc ttcaccctgc tgatgcattc ggcctttgat tccggggctg
    33721 acagtgcggc tgatattatt tcggcaattt ccttcatctc atcttcctta aaacctcttg
    33781 tggtcactgc cggagtaccg atcctcaatc cgctggtgat gaaaggactc tgaggatcaa
    33841 agggaatggt attcttgttc gttgtaatcc cgatatcatc aagcaacctc tccgcatccc
    33901 tgccggttat gccttttctt ctaaggtcaa gaagcatcat atgattgtca gtgccttttg
    33961 agacaatgtc aatatccttg gctgtcaacg aatctgccaa aaccgaagca ttctttagca
    34021 cctgctctat gtatcccctg aattcttcgc tcattgcctc tctgaagcat acagctttgg
    34081 ctgcaattac gtgcataaga ggccctcctt gcattccggg aaaaaccgat ttgtctatag
    34141 ccttcgcata ctgttgtttg catagtatcg caccgcccct ggggcctctc agtgtcttat
    34201 gagtcgttgt agtggcaaag tcggcataag gcacggggct ttgatgtaat ccggctgcaa
    34261 tgattcccgc aatatgcgcc atatctacca ttaagagtgc tccgacctcg tctgctatat
    34321 ctctgaaagc tttaaaatcc aatcgccttg aataggcact tgctcctgcg acaatcaact
    34381 taggccggca ttcttttgca atagccctga cattatcata atcgatcatc tctgtgtttt
    34441 gtgacactcc ataatgaatg aacttaaaaa acttgccgga cacgttgaca ggactgccgt
    34501 gagttaaatg tcccccgtgg gatagatcca ttcccattac cgtatcaccg ggctccagta
    34561 cggtaaaata aactgcttgg ttcgcacttg aacctgaatg aggctgtaca tttgcatgtt
    34621 ccgctccaaa caaggctttc atccgttccc ttgcaatatc ctcaacaata tctacatact
    34681 cacaaccgcc gtaatatctc tttccggggt atccctccgc atatttattc gtaagcactg
    34741 tacccatcgc ttccaataca gccggcgata caaaattttc tgaagctata agctctattt
    34801 tattcctctc tctgtcaagt tcctttataa ttgcttcggc tatttcgcta tcacaagctt
    34861 tgattctgtc aattttcata aaaacttcct cctgtggtgt tttatactat ttactatata
    34921 tcataacaaa gatacgtgct taattgacaa ataccctctt actttaacat tacattttct
    34981 aatgaaaata ttagaagcgc ttatagatgc tataagcgct tcgttttaaa tgtgcttttt
    35041 cagcatatca tcaaaggcct gcttgggcct tgccccgata actccgtcta ccttgctgcc
    35101 tttatcaaac agaaatactg agggtatgct cattatatcg tacctgcctg caagttcggg
    35161 gttttcatca acattaacct ttgctatatc aatctttcct tcatattcat cagccagctg
    35221 gtctatgacg ggtgccacca tcctgcaagg accgcaccat gccgcccaaa aatccaccaa
    35281 taccggcttg tttgatttta acacctcact gtcaaaatta ccttgattta ctatcttaat
    35341 attcttactc acttattagt actcctttca aatctgcagc taatttttat acagattcat
    35401 atacattatt ttatccaata ggccgattat taaattaata aacaatagaa gctgttccat
    35461 taaatactgt cttttgttct tatactaaga ataacaggct tgaatttttt catttttttg
    35521 atagacttat ccttgaattt tattatcaaa taagaaacag ccaatgcagc aagtcccaga
    35581 gcaacgccga ttgcttgcgc cgaattatcc tccaaaccca gagccaaaga accgtaatac
    35641 ccgccaaata ttccggccgt cagtgccagc aaaggaaaac cgtatgcaat agttactgca
    35701 gtcatgaagt tcatgttttc aagctctatt tcaacttcat caccaacctg ggctcccaca
    35761 tcattctcgg ctgtcatcat catctcaagt ttatccttgc ctacctggca ttttccgcat
    35821 tcgccgcagg ctgtgcctct ggtaaatgaa attgttgcct gatttccttc tatctttact
    35881 actttcccta tttctctcat aagtttgtat caatcctttt acttgacata tcttactatt
    35941 gtttcaatta tttcttcaat gcttttgtca ctattctcgt ctttaaggga ttccttgaga
    36001 cactccttta tatacctgtc cataataagg ccgccgactt tgtttattgc ggatcttatg
    36061 gcggaaatct gaaccataat gtctccgcag cacttgtctt cctcaatcat cttttgtatc
    36121 cctttagcct ggccctcaat ctttttcatc cggttaagaa tgtcttcctt acttatctcc
    36181 ttattatcca attcatcgcc tcccataccc cgtaccggta ccctaattac attataatat
    36241 atattaaata tttgtcaata taattttaaa acgtataaca ctaaacacac cgaaagccac
    36301 cgagcccatc cctaatctcg cacctcgtac cccgtacctc gtacctaccc cataggaata
    36361 ccctataaat ctaatatatc ccttgccaca gccgatgcta taataaggcc tgctgccgaa
    36421 ggcacaaagg atatacttcc gggaatctgc cttctttttg tacaatttgc agtctcttta
    36481 ttgctgcaaa tgcagtttgt tttacaatcc gcaacatcct tctttggtga aatgggctcc
    36541 tctttcgagt atacaacctt caggctttta acgccccttt tgcgcagctc atatctcatt
    36601 actttagcca gcgggcacac agatgtctta tatatatcag taatcacaag ctttgtggga
    36661 tcaagcttgt tgcctgctcc catgcagctg ataaccggta tgttcatttc aatgcatttc
    36721 ataacaaggc tgatctttga gctgaccatg tcaattgcat ccacaacata gtcatactcg
    36781 ggaagcagca gcatttccga ggtttcttca ttataaagct ttttatgtac gactatatcg
    36841 gcttccggat taatatccgt gagcctttct ttcattactt ccaccttggg caggcccacc
    36901 gtttttcttg tagcatgtat ttgcctgtta atattggtaa gacagatatt atcatagtct
    36961 acgagcacaa gccttccaat tccgcacctg cacaatgcct cggcggtata ggagccaaca
    37021 ccccctatcc caaaaattgc tacggtgctt tttttaagtt tctcaatacc gtcttttccg
    37081 attaacagct ctgtccttga aaatgcattc atagattacc tccaaagggt ttgtaagaca
    37141 tgggtgggac acggggacgt ttctcctgtc ccgctgaaag cgggacagga gaaacgtccc
    37201 cgtgtcccac cgtccccgtg tccctgctat atttttacct ctatatgaag ttcttccaat
    37261 tgttttggat ctgcggctga gggagcgttt gtcataaggc agcttgctgt ctgtgtcttg
    37321 gggaatgcta ttacatctct tatattgtct gttccggtaa aaagcattac tatcctgtca
    37381 aggccaaagg ccaatcctcc gtgaggagga gtaccatact tgaatgcttc caacagatac
    37441 ccgaacttgc tccaagcctg ttcctgcgtg aagcccaaga gcttgaacat cttctcctga
    37501 agctcctgcc tgtgaatcct tatgcttcct cctccaagct cgtagccgtt taataccaca
    37561 tcataagcct ttgcccttac tttcagcggg tcggtgtcca aaagctccaa atcctcgtcc
    37621 atggggcttg taaacggatg atgcattgct acgtatctgt tttcttcttc gtcataatcc
    37681 aaaagcggga actcggttac ccacaaaagg tcatactttg tattgtcaat cagcccaaag
    37741 cgttttgcca attccagcct caattgcccc agggaatcaa aaaccacctt cggtttatca
    37801 gatacaataa aaattatatc tcctagtttc ccacccactc tgtcaattat accgttaagt
    37861 tcataatcct tcaaaaactt tgctataggc gattttacgc cctcagaagt tatattaatc
    37921 catgcaaggc ctttggctct gtatgtcttt acaaactccc caaggctgtc tatatctttg
    37981 cggctgagct ttgctccgag cccctcagca tttatagccc ttatgattcc gccatttgca
    38041 cgggtctccg taaatacctt aaactccgaa tttgcaacca ggtctcctat atctttcagc
    38101 ttcagaccga accttgtatc cggcttgtca gaaccgtaat gctccattgc ttccttgtat
    38161 gtcagtctct tgaaaggtgt ttgtatatca acgtcaagaa cttctttgaa cagtctcttt
    38221 aacagccctt cattaacctc cattacatct tccttttcaa cgaaggaaag ctccatatca
    38281 atctgagtaa actccggctg cctgtcggcc cgaaggtctt catccctgaa acacttggct
    38341 atttgaaagt atctgtcgaa gccggacacc ataagcagct gtttgaaaag ctgcggagac
    38401 tgaggcagtg caaagaaatt ccccggctgc accctgcttg gaaccaaata atccctggca
    38461 ccttccggtg atgtcctgat aagtatcggc gtttccactt ccacaaatcc gttttcatcc
    38521 atataatccc tggttacctt tgcgattttg tgcctggtga ataaaaactt ctgcatatgc
    38581 gggcgcctca agtccagata cctgtatttc agccgcagct gctcagaagc attcaggtca
    38641 tcctcaatat aaataggtgt tgtctcagcc cggctcaata tttcaaggct gctgacagct
    38701 atttcgatat agcctgtcgg catcttcgga ttaattgcat tttcatctct ttttataact
    38761 tcgccggata tcttcagcac atattcactt ctgactttaa aagcttcggc aaatacatca
    38821 ggagtggcat ccttgttgac ggtgcattgt ataataccgc tcctgtccct taactgtatg
    38881 aatatgagtc ctccaaggtc ccttctcctc tgaacccagc cggcaagtgt caccttttcc
    38941 cccaagttat ccatgtttaa ctctccgcac atatgtgttc tgttgattct tgccataatt
    39001 tcatctccta catgtttttt atatagcccg caagtgcatc aaggctgatt tcttcttcgc
    39061 tgcccttatt catatccttc accttgattt ttccgctttt tatctcttct tcccccaata
    39121 ccgccacata tcttgcatta atcttatttg catatttcat ctgagccttc aggcttctcc
    39181 ccatatagtc cttctcagcc tttatgcctt cttttctcag gaggttgact tgcctgagcg
    39241 cttcttccgc tgcttctttt cctatgtttg caatataaac atccatgtaa acaggctccg
    39301 gtatttttat tccctgattt tcaaccacaa gaagcagcct ttccaatcca agcccgaagc
    39361 ctattccagg cgtgctgggg ccgccgcatt cctctatcat cccgtcatac ctgccgcctc
    39421 cgcaaactgt gccctgagac cctatatccc tggacacgat ttcaaaggct gtcttggtat
    39481 aatagtcaag gccccttact atttgagggt caataacata ttccagtcca agagcctcca
    39541 gggatctttt caggccttca aaatgctcgc cgcattcgct gcagacatta tccagcatgt
    39601 aaggaacatc agcaagctgc tccttgcagc tttcctcctt gcagtcaatc actctcatgg
    39661 ggttcttatg gaatctgtcc ttacatgttt cacagagctt atcaattttg cccgataaat
    39721 actccctcag cttcttattg tattccgccc tgcatttggg gcatcctacg ctatttatcc
    39781 ttatttccag gtttttaact ccaagcctgc cgtacagaat catggcaata ttgattatct
    39841 cggcatctac ggaggggtcg gaactgccga aagcctcaat atcaaactga tggaactccc
    39901 tgagccttcc ggattgaggt ttttcatacc tgaaacaagc tgttgtacaa aaaactttta
    39961 ccggctgaat atcattaaac atgctgtgct ctatataagc ccttacaaat ggcgctgtcg
    40021 cttccggctt aagggttatg cttcttcctc ccttatcctt cattgtatac atctcttttt
    40081 gcacaacatc cgtagtatcc cctattcctc tttcaaaaag ctcggtatgc tcgaaaatgg
    40141 gtgttctcat ctccttatat ccgtgcaagg cgcaaatttc ccttattaag tcttctacat
    40201 actgccattt gtatgattct gacggcagta catcctttgt gcccttgggc gcttgcgtta
    40261 acatataaat cactcctccg acaatatttg ttctaaaaca tcaagcttct tccttatcat
    40321 tgcttcgata ttctcaatat aaagctttat atcctttata ttgtgctgcc tttctaacct
    40381 gttttcactg tttatgacaa ccttggtcac agcatctgct ccaaaggcaa caatggtttg
    40441 cctctcttca ataatctgca tattatatat acattcaaag cctttttttg aaaagccaat
    40501 gttctcaagg ttttgtacca tatgcttctg cctgtacagg taataaggat gcatacccat
    40561 atccccaaga aaaccttttg tatattccat catccgggat acattttcat catcgggcaa
    40621 agcacttttc atatactttt cgttcaatac cgaagccctt ttcaatgcca tagtatgcac
    40681 cgtaacgctc tcagggtcaa gcttttttat ttcttccaga gtatgagcca attccgtcat
    40741 tgtttccccc ggaaggccta atattatatc catattgata ttaccaaaac ccatttcctt
    40801 tgcaagcctg aagctctcta taacctgtcc cggatcatga gccctcccta tacgcattag
    40861 ggtatcatca ttcatggtct gcgggtttat gcttatcctg cctatattat tctcatacaa
    40921 tacctgaagc ttttcttttg ttatggaatc aggccggcca gcttcacagg tgaattcaca
    40981 ttcctgcctt ccaaagctgt tgccgatgca tttaatcagc ctttcaagct gttgtgcgtt
    41041 cagggcagtg ggagtgcctc ccccgatgta tatggtctgc accttaaagc cactggtgtt
    41101 taaaaactcc gaaacccttt tgatttcttc cgtgagtttt tccatgtagg gctctacgta
    41161 tccgcgatac gcttttattg aatttgaggt aaatgagcaa tattcgcatc tgctggggca
    41221 aaagggtatt cctatgtata tgcttatctc cctattatta taagggtata caaagggtct
    41281 ttcaatatct gcgattctga ctgccagttc agctctatcc ctgctgacaa gatattcttc
    41341 tgccagcctt tcaggtatat cggttgtaga aaccgcatta ttcataagct catgaacaat
    41401 tttcaccggc cttattccgg taagtacccc ccatgggtat tcccttttga atactgccgc
    41461 cagaagcttg tacatggatt ttttgaccag gcgcttcgct tcattgccat ccgcggccct
    41521 tattccactt ttttcaacag cttcttcatg cttttgccct tccaggtatg tagatacgca
    41581 tacagcgatt tcaccgttta tatccagcct gctggttatc aaaatgcctg tatcctgcaa
    41641 aaagctttgg ctgtcagcca aagctccctc agaccttact atctcgcact tgccgaaata
    41701 cattcttact atatcggcag cctcattata atattcatgg ttaatcagtc tgatttcaat
    41761 catagcttcc cctaaatatc atcattgtat atttgattct taacctccga aacaatacct
    41821 gaagctattc ccaattccgt ggctatttca aaatcttcca ttccggattc tatcatatca
    41881 ataagctcat tatattcctg atacatccta ttcttatgct tactggccaa gattttttta
    41941 gaagacttat tcatataaaa ccctcccatg aaacaggtat ttattatcat agtctttcct
    42001 tttgccccaa gcatattcat ttaataatca atcaaacaaa gctgttttcc tgcttttcat
    42061 gcaataatgc tgttgaaggc ccgtggccgg gataaaccat tgtgtcgtca ggcagagtaa
    42121 aaagcttttc tcttattgag ctaaccagtt cagtcatgga tcctccgtac aaatctgtcc
    42181 tcccaacaga gtttttaaac aaggtatccc ctgaaaacaa tactttgtca caataaatgc
    42241 ttatacctcc ccgggagtgt cccggcgtat gtattatatt gagcttcgta tcgccaagct
    42301 caagtacttc cccgtcttca aggtacttat ccgctgtcat ttcaatcgct gctccgccca
    42361 acatgcccga aaaattcatt gttttgctct tcagcatttc actgtcttct ctatgtatgc
    42421 aaactaaagc gcgggaatac tccttcagtt ccttcagggc accaatatgg tctccgtggg
    42481 catgggtgat tattatatat tttatattta atccgttggc ttcaatatag ctttttattg
    42541 cgggaaaatc ccctccggga tcaattactg ccgtttcctt tgttttctcg tcataaacaa
    42601 tatagcagtt ggtttgatat attcctaaag ccagactttt aaatttcatc atataccagc
    42661 acgtctcctt ctttatatta tcttctcact gtccacaatt actgtgacag gtccgtcatt
    42721 ttcaatatta accttcatat atgcctgaaa ggttcctgtt tcagttttca ctcccagttc
    42781 cctgcacttc ttgacaaact cattgtaaag ctcttcagcc ttttcgggcc ttgctgcact
    42841 cataaaattc ggccttcttc ccttcctgca gtctccatac aatgtaaatt gtgaaatcac
    42901 aagcaactca cccttaatat ccagcagaga taagttcatc ttctcattat catcctcaaa
    42961 tatcctcaaa ttcactgtct tgtcaaccat gtacagcaca tccgcctcgg tatccttctc
    43021 gtttactccc agtaaaacga caaagccctt gcctatgctt cctactattt tgtcatcaac
    43081 agcaacactt ccgctgttaa ccctttgtat aaccgcccgc attatttacc ctcctgtaac
    43141 tttgcatcat tgctttgctc tgaagacatc cactactcct tcgattcttc tgaactttct
    43201 tataagctta ttcagctgtt caatatcggt aatatccaaa atgaaattga tgattgccat
    43261 cttatccttt gttattctgc aatgaaagga actgatattt atcttgcttt cattaataat
    43321 cgtagttacc tcggcaagca atcccttcct gtccattgcc cttatttcca catcagcgct
    43381 gtatgattcc tttgtctggc tgctccattc cacctctatg aatctttctt cctcgttcaa
    43441 aaggccggct acattggggc agtccttctg atgcaccgac actcctcttc ccttggtaat
    43501 atacccgaca atttcgtcac cgggcacagg gttgcagcac tttgaaaacc ttatcaatat
    43561 gttctccacg ccttttacct tgactcctgt gctgttatac cttttcttcc tttcctgagc
    43621 cttctcagcc tggcgttcga tgctatcctc agtaatctct tcaagcttct gggtttttct
    43681 gaattcttcc ttcagttttg ctattatctg attgggtgtc aaacctccat agccaaggga
    43741 tgcgtacata tcctccaaac tgtggaggct gaatttctta tatatggcct caacccattc
    43801 gggcctcatc aactgaacgg tggtatatcc gtttctcctt atctccttct ccagcagttc
    43861 ttttcccttt tgaatattct cttctctttt ttccttctta aaccattgtc ttatcttatt
    43921 ctttgcctgt gagcttttga ctattttcag ccagtccctg cccggtccgt tggcaacggc
    43981 agaagtaatt acttctacga tatccccgtt ctgaagctta taatccaatg gaactattct
    44041 tccgtttatc tttgctccta tgcacctgtt gcctatttgg ctgtgtattt tatatgcaaa
    44101 atctatcgga gtagattcca caggcagatc tacaacatca ccctttggtg tgaatacata
    44161 tacttcatcg gtaaacaagt ccaccttcag ggtctccatg aactctctcg tgtccttgat
    44221 ttcgttctgc cattcaagca tctgtctcag ccatttcagc ttttcgtcaa actcggactg
    44281 ggaggtcttg ccttctttat atttccaatg agccgcaatg cctatttcag atgtcctgtg
    44341 catttcccat gtccttattt gtatttcaaa gggctgtccc ccaaaaccca gcaatgtcgt
    44401 atgaagggat tggtacatat ttggcttcgg catcgcaaca taatccttaa accttcccgg
    44461 tatgggcttc cataaggtat gcactatgcc cagcactcca taacaatctt ttaccgtgtc
    44521 cactattatt cttactgccg taaggtcgaa tatctgctca aaggtcttgt tctgatagta
    44581 cattttcctg tatatgctgt aaaagctttt cggccttccc tcaatatgag cctctattcc
    44641 tacttcctcc agctttccct taaggacctc cataacctga ttgatttcat cctcgcgttc
    44701 cttcctcttc ttggcaacct tgtccaccaa ttcgtaatac ccttttggtt caaggtatct
    44761 taacgccagg tcttccatct cccactttac cttagatata ccgagcctat gagcaatagg
    44821 agcatatatt tccattgtct ccctggcctt ttccttctgc ttttcaggag acaggaatct
    44881 aagagtacgc atattgtgca gcctgtcggc aagctttatc ataatcaccc ttatatcctt
    44941 agccatagct atgaacatct ttctgaggct ttccgcctgt tgctcctctt ttgtcttgta
    45001 ttcaagcttt cccaatttgg tgaccccatc tacaagcatt gcaatctctt cgccgaaaag
    45061 gttcttgcaa tcctcatagg tgcattttgt gtcttcaata acatcatgaa gaaggcttgc
    45121 agcaatagtg atgttgtcca tattaagctc ggccagtatt ttcgccactt ccagggggtg
    45181 aaaaatatag tcctccccgg atactctctt ttgtccgtca tgagcccggg cagcataatt
    45241 ataagccctt tcgacaatct ctgcgtcacc ggtcgggtta tactttttaa ttattttcaa
    45301 taaatcttct aacatagcaa caccaacttt taaacgttaa atggactgcg tatacaagta
    45361 ctactaaaaa agctggttgc gccagctttc atatatttac atttatttta gtataaattc
    45421 cttcaattat caaatataaa taataactct ccaatattaa tattttataa tcgaacttac
    45481 atcataccct tttaaattct ctcttccttt caagaattcc aattctataa gaaatgcaat
    45541 gtgcactact tctcctccaa gctgttccac caatctgacc gcagacaata cagtacctcc
    45601 ggttgcaagc aggtcatcca ccacagccac tctcgtacct ttttttattg catctttgtg
    45661 aatctccagg gcatccattc catattccag ctcatactta tagcaggctg tctcagcagg
    45721 aagcttgccc ggtttcctta tgggaacaaa accaacccca agctcatatg ctgcaggtgc
    45781 tccgaaaatg aaccccctgg cttccggtcc tacgataata tcgaaatcat aattacttaa
    45841 attatctgca atggatttca cagcgtattt gaaagcatct ccctctttaa gaagggttgt
    45901 cacatccttg aagcttatgc cttccttcgg gaaattttca attgttctta tatattttat
    45961 taaatccata aatactaccc ccatttatta atttatgagt tttatatcct ttatgttcag
    46021 ctggacactt tctgttccgt tccatatgtt attctcaata ctacatatta tatcaatttt
    46081 ttcgccgatt tgtaatattt tttgcatatt tcccaaatta taacctatgc agctgatttc
    46141 cttttcgccg tcaaaaacct ttagagagag gtgtctgcta tcattcccaa ctcctttgaa
    46201 agatgtgacc gaaagctccc tgaacacata aagagggact gtattgcctg tgccaaaggg
    46261 ttccatcagc ttcagcagtt ttgcagtatc aagggtaacg tctttcggct caagcttata
    46321 atcaacaaat atttccggta tgagcttatc ttcgcctaaa gtattttcaa cttcaaaatt
    46381 catcctctcc ctgagttcgt ctattttttc ggttcgtata gtaaagcctg cagccatttc
    46441 atgtccgcca tacctttcaa aaagatatcc gtgtctggac attgcctcaa aaatattgaa
    46501 accgggtatg cttcttgctg accctttgca gaactccccg tcacaaacca taataaacgc
    46561 agggagataa aatttatctg ccagcctcga tgccacaatt ccaataacac ctatatgcca
    46621 atcctccctg cacagaacca ataccttatc cctatccaaa tcaacgcttt cacctatcat
    46681 agcttctgca cagttcaata ttccattttc tatcgattgg cgcatcttat tgtctgaatc
    46741 cagcttggca gctgtttcca ttgccttttc cgcattatcg gtcagcaata gctcaacaca
    46801 ttcgccggca tcgtcgattc tgcccgctgc atttattctt ggagcaagca tgaaggccac
    46861 attatatgaa ttcagctctt tgccctttaa tccgcatacc gcaagaagcg cttttatgcc
    46921 ttgactgtcg gtattttgga tacttgcaag gccgtttttc acaattatac ggttttctcc
    46981 caacaatgga actacgtctg ctatcgttcc gattgcagca atatccaaaa gcccttctgc
    47041 aaaaccgggg ctgcctgctc tgcaaagcag tgcgccggca agcttgaacg caaccccgac
    47101 tccggccaga tccttgaagg gatacccgca gcccggctga tgcggattga tgatgcttaa
    47161 agcatccggc agctcttccc cgcactcgtg gtgatccgtt ataatgatat ctattcccag
    47221 ttcctttgca taatcaactt cctcacagga ctttataccg cagtctacgg taactatcag
    47281 gcttaccccc atctccttaa ttctgtcaat tgagttgagg ttcattccgt agccctcttc
    47341 caatctgttc ggtatatagt ataccccgtc agccccaagc ttcctcaatg tctttatcat
    47401 tacagcggtt gaggtaattc cgtctacatc gtagtcaccg taaatgcata ttttctcatt
    47461 gcttctgact gccttaaata tcctgtttgc tgcctgctcc attcccatca aaaggtacgg
    47521 atcatgaagt gagcaaaggc tggtgttaaa aaattccctt gctttcggaa cagtatcgat
    47581 acctctgttt atcatagctc tgataattat ctccggtaat tgaaactcct caattatcgg
    47641 attaaggcta tccatgcttt tgctgtaagc tgtccatctt ttgttcaata ttcatcacat
    47701 gccttactga atttctggtt taaggttatg aaatatccat tttatctata tcttctattg
    47761 tctgaggctt ttcctgcata acaggttcct ttgccggaag cttttccgac tcaatccttt
    47821 tgagttcttc cttaatcttt ttgttttctc ctgtcagctc cctaagcttg aatttgtatt
    47881 ttattcttct tataatatcg aaaggaagca taatcaatac cccaatcaat gcagaacaaa
    47941 gaattaccac agcttgtgac aggcttacct tataccagag aaaatttatg acaactactc
    48001 ctgcgttttg taccgcaaaa aatgcaataa taacagcaaa aaccaatgaa attacaatag
    48061 taaattgcat ataaaaccct cccgccatta ttgattaaat atatgtaagt caccaagctt
    48121 ccatgcataa agtatacctt cactgctgca aaaacttgat actttaagtc taatatatag
    48181 cttttcggcc aatacttcaa ctgttttcta acagtcctgc tgcacaatac aagaagaatg
    48241 cgacgcatct gtagtttgca tagacgcggg gacatgggga cgtttcccct gtcccgcttt
    48301 cagtgggaca cgcgaaacgt ccccgtgtcc ccgcgtcccc atgtccccgt cgcattattc
    48361 ttctaatgcg aaaagggata tcgctggcta cttatcaagt agtcttgcga caccccttta
    48421 ataacctgta tcagcgggct ctgcttactt taaatctcct tctcagtatt acccataacg
    48481 ggcttgcaat gaatatggtg gaatagcatc cgcttactat acctataatc atcggaaatg
    48541 cgaaatccct tattgcaggt acacccaaga aatacaaggc aaatattgta attaaagtag
    48601 tcagtgaagt atttattgaa cgggcaaggg tctgagtgac cgatacgttg accagattat
    48661 cgtaatcaat cttcttgctg tgttcgttct tcaaattctc tctgatttta tcaaagatta
    48721 ctatagtatc atttattgag tagcctatta ttgtcaataa cgcagcgata aatgtactgt
    48781 tgaccggtat ctggaaaacg gcatacgctg caaccatcat tgcagtatcg tgtattaatg
    48841 ccgacaaaga agccagtcca aacctccact caaacctgaa ggatacataa atcattattc
    48901 ctgcgaatgc aagcattact gcaataagtg actgcatagc caattctttt cctacggtag
    48961 gccctattgt ttcaaaagaa atattatttg attctattga atacttatct gaaaaggctt
    49021 ttattatctc tgcccttttt tcattatcaa agtttatctt ggttcttact atgacctgat
    49081 ccttgttttg tcctgcatga gttattgttg ct
//
LOCUS       DAOQUR010000016        48686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_813_length_48686_cov_10.326582, whole genome shotgun sequence.
ACCESSION   DAOQUR010000016 DAOQUR010000000
VERSION     DAOQUR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 48686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48686
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_813_length_48686_cov_10.326582"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..556)
                     /locus_tag="PKI50_05225"
     CDS             complement(299..556)
                     /locus_tag="PKI50_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03146.1"
                     /translation="MKKKRKSNCKGRGFEQISFRDIDLRIGDKLYKDGRLYAEVMGES
                     EELYFLQKSGSSCEMPNPYFKETIIENILFGRLFLEEMRFQ"
     gene            807..2063
                     /locus_tag="PKI50_05230"
     CDS             807..2063
                     /locus_tag="PKI50_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03147.1"
                     /translation="MRLLSALYHEGIEEYIESIPGIEVLDVVGNKRSLLDSLSKDKYD
                     LVLISKSLPGNEEADLLIEMLASEEFKGLRTAYLYGEYDGYCDDFIKFLINHGIYDFH
                     VGDEITSKDIERIIFKPASKERALYYFRSHYDNEMNIHYPEQKNKRNKRFFEGRFLFN
                     SGFGSLFSKNHKKNPKEKLIISIISNQATGKSHTAWNLSHCFSKLGYATSLFNIDRGY
                     SANLFFDIDEVYYNLLDFTLQNDIHKDILENCYNRKNLCVVTGKLGDEKEISSEDFLK
                     LLYCIRTKSDITIIDTRTGISELTRLSMQNSIYDMLVFDCDIMHFHMNMKMLEELKDD
                     FVPEKTIAVINNTNIRSSSHKFIYNELTSSGIPFKDMAFVSSCGFISYEVMHTGYTPY
                     QAAADRNKSFLGDMDSLLDKLGISNE"
     gene            2078..2767
                     /locus_tag="PKI50_05235"
     CDS             2078..2767
                     /locus_tag="PKI50_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNT03148.1"
                     /translation="MAYSKKNKVFAVKRTALISGCVVCAVLILSAVYMLYGNVFSGEA
                     AGTVRNSSENEAKTVVIARAKENLGQGELLAASMAELIEIPVELAPEGAIKSLSKLNN
                     MRLKRGIAKNEFFNDMDLMPQNAFYEKGERLIEHNFAEGAVPASVTAGSIIDIKLFVK
                     GGEDGIVVSKAAVISRSGNLLSFYLDGMEQEFIKEAAAEGALFAVQYIDASQQASEVT
                     YTPSYSKDKQN"
     gene            2783..3634
                     /locus_tag="PKI50_05240"
     CDS             2783..3634
                     /locus_tag="PKI50_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03149.1"
                     /translation="MRGAKGKLICTWSPMLHGEGCSTLACGIGIGLQYLSGKKVLIVN
                     KSNSASHMERYVEKDIEIKYSMDNLKIFNKEIKTEHIFTYATRINKDLYMIAGSRLNR
                     DIIGENSEFDKIFLEKCLAGFDLVITDLDTGVRDDNRTYLDQADKILAVVTPNEIIID
                     KLYDEYRVRDALGYFTDDKAVVIINKLCEGWDTAGVIGRYKNRYSLSDIFGLNYDGDV
                     LNACCTDKNFYSFLMKEAKREKNIFIKQLSEICRFLIEGLCMGNNAADRIKHGNIFKK
                     FLKSSIY"
     gene            3651..5387
                     /locus_tag="PKI50_05245"
     CDS             3651..5387
                     /locus_tag="PKI50_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT03150.1"
                     /translation="MDLGKYLIIDSLCLHMEKYINSFINSEPSDEQEKSIVSLLRDYK
                     EKSTSGLPEAREVIKSHIKTGILTGFDIYVSNEMGRLEDAIMLKGVCVDEANGLADLI
                     LPFNEPQKLTSREKFEIILHKNRNSSGNGRDGAFKAILARYSCCCKAREAAGYRSLRY
                     EYDEADIDYIYNNEDYVLSFSDKIEIITQRLYEEIFGLKHIDMLAYSDINEVGFSNNG
                     RYVYCWSGKKIWLSFLQISEKDARVIQDRAISFEKHCPQLDVSHPEILCHRGDGARIT
                     VTQKPYFSARNLCIRIFNQSCADFNDLITADKLRTLIIALVKTGESICLQGGLGTGKT
                     TTMNVMYELLDDLLHIGTIEDYFEQHIMEKYPYKRIVEGQAVNGKDLLDVVKTMLRMS
                     VDVANIGEVRDGNALFAFIQLVQSVSVAAWFTTHITNPETTVPRLKNMLIGTGRYFSE
                     QSAIMDIVHYINMIFQHEIIDGRITITKAVEIVPLVAVSSDGEYDIETPREQLEKLFY
                     IQQLRSNTYNMFRLNTIMQSVDGKAKFVNYPSRRMVKKAKGDRQSWEYLKNLIRLIEK
                     DTGMAIPEYARG"
     gene            5396..6220
                     /locus_tag="PKI50_05250"
     CDS             5396..6220
                     /locus_tag="PKI50_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03151.1"
                     /translation="MRNYYSKGIFHALFAGLNNRLTRINQKSPAQNARTSLALLTVLT
                     ILVIIYFLLACSTFKGLWYHRVLCGFSAFIFMYLCKESYFEIYEKRIKRDLPNTLKKL
                     THYYNHYRGNIIPALEDTVSRCPRSNRIYMIEIREALMKPDYQVQIEELEGKMPMIWL
                     KMLCRLILSAKENGGMSSEGDTVANNLKRMTNIITFLNIEQGYNDAELLGMQIFVFFT
                     PFLVIPLTKWYNISLLSGLNIGDIYGSIQAQGLTAIMMFISGVGTIFIHWMRKLQN"
     gene            6196..7395
                     /locus_tag="PKI50_05255"
     CDS             6196..7395
                     /locus_tag="PKI50_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03152.1"
                     /translation="MDEKASKLIKGLSNIQYFILIQAIAIALLIAAASIFVHQYFRYR
                     KIKRLYRADYARKSCLKSISRFLIFMPGYKSRRYKNTEGLIINSGLRVSVEEFYLAKT
                     LLFVLGFVFFISVQTTNTFAFYDHVISDLNMGNTLIDEAAPNDYTLQLEKEVFIHIDS
                     SLPKGKAALKELKDENNSQVYIGYIEAQISSKWAGLEEYAGEMAERMYKKLIKIRRIE
                     TDYLIYLTSFILAIILYFIPNIIGHLKLILIEDKRDWEILNYIYVFSMFGRMPPFNIK
                     NVLSNILVISDIYKPIINEALNNLNSGKGEEAFDNLLQKVENQELYELLESMRLSMST
                     GLLNIVDNIDEMAVNQLKWLEIKSLKRRKSKQVIAMVPVVLVMLAAAVYFSYSLSTLS
                     NPMNYLK"
     gene            7415..7771
                     /locus_tag="PKI50_05260"
     CDS             7415..7771
                     /locus_tag="PKI50_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03153.1"
                     /translation="MEKFWAVILIFVVVAAAGLMYVNQVYIPQKETQMAMADMANQTL
                     SAVKVEIGDDSVPQEVSGSEIKDIIEDYSDDDIKVYINGIIYDGTNVIEHSSQYYMRV
                     IKNDGEITEIRFTIIV"
     gene            7922..8368
                     /locus_tag="PKI50_05265"
     CDS             7922..8368
                     /locus_tag="PKI50_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03154.1"
                     /translation="MEKFWASILVIPIFTWYSFHAVLDRDFRIKQQAVENIVCQYTQI
                     AAKKGALYNSVYNEMKERLSRFGTFDISLTAERFTGSSDTPELIKGSYIIGRDLRSEG
                     FDIINIYAESINRHPLGRLYELTPFGTKSGAEADIRYFGKASVYIQ"
     gene            8385..9173
                     /locus_tag="PKI50_05270"
     CDS             8385..9173
                     /locus_tag="PKI50_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03155.1"
                     /translation="MKSFFWSLIFIFCTWSMFFIGKGVDNIDIMAERYKRALDAGANA
                     ASGYRAYYSEKMLYNHGTGFGIGLEDSNNLPVDREEAVKWFYRLFFRNLGIEDTNKQN
                     EYKRYMPMKAIILFDRLMIADADDNWHTYDPAGEKEYMIQYRGKDYKFTLSDQVYDIE
                     NSSWIMADDIGLEAKERKELVTNYIINELNSFLDNRGNKESGSYYKIVFSLDDASDSK
                     LSGINGINFFILCEGIPIPSLNPFKQEKFFAYSVGGSEIKKKQL"
     gene            9425..11329
                     /locus_tag="PKI50_05275"
     CDS             9425..11329
                     /locus_tag="PKI50_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNT03156.1"
                     /translation="MSVLPYITAVDNRVEDVNKPFLDLTGYTESSIRGRNVEEIWQQL
                     LRISMDLDSVKCLKECSCYMFDILRRAKEVTISYTADFELKQCIYTFKRKVNSMLEDK
                     FPFLEHANSANDTGVALYSVPEFILLKGNESHNRFLSRLLGGAEDIIGKRIEEILPDW
                     KGSDAENIFSNVVKTGKPIFYDEHMREHPSTGIVCTRYTLTPIFEGEELRYLVLIHND
                     VTKNLTIEREKDYFEKQRDYLYRLFNAMNLPIISFSYPEYRIKEFNNKALVELSELTG
                     MDNLTIDPSISCKGLAEMIPLFDISSAKILIEQMKQTKSPVCHKKMELCKKGRKVYYD
                     VTFHPFLNIMEEISEILIVAADVTDEVEKAKHIENILKLKDEFLYFITHEFKTPLSVI
                     NAAVQTLEHCYSMQIPDKARFLIGKVKQNSYRQLRLVNNLLEIARINSGYIKLRQRNV
                     DIVFLTRAITESVAIYAQQKGIELVFVTKFPQRVIGLDDEKLERVLLNLLSNAIKFTP
                     AGGKVVVELSSKLHKHKRVICIKVKDQGVGIPKEKQSVIFDRFGQVDNLLTVQAEGSG
                     IGLYLVKLMVDSIGGEITLESEWGKGSVFTLMIPSKKSKENAKEQKFKLRSDNRLIQT
                     TAIEFSDIYL"
     gene            complement(11334..11807)
                     /locus_tag="PKI50_05280"
     CDS             complement(11334..11807)
                     /locus_tag="PKI50_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNT03157.1"
                     /translation="MVSKSIISKKIYEMLVNHLVRIEEEKLHVLDKYYPVWTNERNDF
                     QELLDSYIKGVENIIYNEVLVDDNASDTCPFAIIGSQVILKDCYTNKIEEMQIVSPFM
                     GEADLNIDSASYLSPMGRAFLLKKVKDDVKVETPMGIFQYIVENITLPSDAIDSN"
     gene            12377..13012
                     /gene="cysE"
                     /locus_tag="PKI50_05285"
     CDS             12377..13012
                     /gene="cysE"
                     /locus_tag="PKI50_05285"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNT03158.1"
                     /translation="MFGRLKEDIRTIFERDPAAKSYLEVILCYPGLHAVICHRIAHGL
                     YRRRRYVAARLISQISRFFTGIEIHPGAAIGRRFFIDHGSGIVIGETTEIGDDVTLYQ
                     GVTLGGTGKDKGKRHPTIGNNVTIGSGAKILGPISIGHNAKIGAGAVVVRCIPPNSTA
                     VGVPAHVVGREGIDETYVDLNHTDIPDPLEDEIRELEKRIRELERKLFSID"
     gene            13096..13902
                     /gene="pgeF"
                     /locus_tag="PKI50_05290"
     CDS             13096..13902
                     /gene="pgeF"
                     /locus_tag="PKI50_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNT03159.1"
                     /translation="MKDGLAYLTIPAFEKTGMVKHCFTTRIGGVSRGIYNSLNTSDDK
                     EDPVENVHRNLKLVCGAIGIDYSNLVFSDQTHEDNIRVVSIADLGKGISVPNDIKNTD
                     GLMTNIPGIPLITFYADCVPLFFLDKENKAIAVVHSGWKGTVQKIGVKTIRQMTEVYG
                     TKPEDCLAGIGPSIGPECFEVGQEVADQFTESFGTRPHIIKPLGNGKYMVDLWAANKL
                     MLMEMGVPENNITISGLCTKCREDLFFSYRRDKGRTGSMSAIMELKEEDT"
     gene            13902..14621
                     /locus_tag="PKI50_05295"
     CDS             13902..14621
                     /locus_tag="PKI50_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT03160.1"
                     /translation="MNRKILIIEDEANIQELLRYNLEKNGYIVTISDNGIDGLQKALD
                     QVPDLILLDLMLPGLDGLEVCKRLRMQKATRRIPIFMLTAKSEELDKILGLELGADDY
                     ITKPFSIKELIARIRAAMRRLDDESSAVSDKAEKNKILKARDIEIDSEKYEVRKNNEK
                     ITLTLKEFELLKMLVENKGKVLTRDFLLDKIWGYEYMGDTRTVDVHIRHVRQKLNDDE
                     GSGRMIETIRGVGYRFISEGE"
     gene            14624..16393
                     /locus_tag="PKI50_05300"
     CDS             14624..16393
                     /locus_tag="PKI50_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03161.1"
                     /translation="MQRKIFFNIVVVLLLGVIISGLLSARIVKKNLLSDIEKSLTTEA
                     ALVRELLGESLINNSNDIGIYVGKIKQTANSRITIVDVMGNVIIDTDKEYSEMENHAG
                     RPEIAAALKGEAESSIRYSSTLRVDFIYVAQPILKGSSIIGAVRLSKPLYEVKNMLNS
                     LYINVFIAVLIGIFVASVLGYKMAMNITKPIKEITYTASRIAKGQLDRRINITSRNEI
                     GILADSINDMASKLRDTITSLQDKNIKLESIMSSMINGIIAIDNSEKILFINHVAESL
                     LGITGGEITGKHLLQVVRNNSIDNYFKKMLKDKEFFNTEVNLNDSNEKILKFHANSIK
                     QNGENGIEGIIITIQDVTELRKLERVRTEFIANVSHELKTPLTSIKGFTETLKSFDFD
                     DKQDAIRFLNIIEDEADRLYRLINDILSLSELEQKKTKTVKEKIDIEKAVEEVLSVLK
                     SQSDKKNIELSAKIQEGLESISGDGDKFKQMLINLIDNAVKYTPENGKVSIEAYKQSH
                     NADLDKLIIKVKDNGIGIPKQHIPRLFERFYRVDKARSRTIGGTGLGLAIVKHIVMLF
                     NGEIEVTSEVGKGTEFRIILPMK"
     gene            16550..17404
                     /locus_tag="PKI50_05305"
     CDS             16550..17404
                     /locus_tag="PKI50_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT03162.1"
                     /translation="MRIKKTLAFICAAVLLIGALTGCAPAETNEPAANGLGESITING
                     STSVQPLAEELAKAFKEKNPDATIDIQGGGSGVGIKSAADGVADIGMSSRDLKPEEKS
                     LKEFKIAVDGIAVIVNPTNEIQGLSMEQILKIYTGEITDWKEVGGKEGKITVVTREEG
                     SGTRGAFIELTKVESKSEGDRTVASAITQGSTGAVITTVAGDPNAIGYASFGSAKENT
                     DIKLISVDGKTCTEENIYAGEYKISRPFLMLTQAEPTGLAKAFLDFILSEEGQTVVSG
                     HNYLKAVK"
     gene            17425..18273
                     /gene="pstC"
                     /locus_tag="PKI50_05310"
     CDS             17425..18273
                     /gene="pstC"
                     /locus_tag="PKI50_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNT03163.1"
                     /translation="MKAYERVIQLVLFISAIIAVFGVILISYFIISEGYPVIRKVGLL
                     DFLSGNRWAPTQGVYGIYPMIVGSLLVTAGALVLGVPIGIGCAIFLAELAKPRIAGIL
                     RPGIELLAGIPSVIYGLYGLAVIVPFIRKVFNNQGFSVLAGSIVLAVMILPTIINVSE
                     SAIRSVPRDYKEASLSLGSSHWQSIKNVIVPAARSGITAAVILGMGRAIGETMAVIMI
                     VGNSPLVPESILSPVRTLTGNIGIEMGYAGGEHQKALFATGIVLFVFIMLLNIAAHLI
                     PKKAGE"
     gene            18314..19132
                     /gene="pstA"
                     /locus_tag="PKI50_05315"
     CDS             18314..19132
                     /gene="pstA"
                     /locus_tag="PKI50_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNT03164.1"
                     /translation="MRKNEKTAFIFLWAAAAITVLTLIAIVGSVLINGIGHLSLDFLT
                     KEPENMGKDGGIFSIIITTIYLALFSLLIAAPIGISAAIFLTEYAKEGPVIRLIRFGT
                     ETLAGIPSIIFGLFGYVFFVVFLKFRYSMVSGGLTLTFMILPTIIRTAEESIKMVPMS
                     YREGSLALGATKWQTIYKVVLPASIPGILTGVILGIGRVVGETAAVIYTAGSSLGLPN
                     SIWRPGRTMAVHLYILASEGLSKSNMYATATVLIITVLIINFTANRLIKQFIRS"
     gene            19148..19900
                     /gene="pstB"
                     /locus_tag="PKI50_05320"
     CDS             19148..19900
                     /gene="pstB"
                     /locus_tag="PKI50_05320"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNT03165.1"
                     /translation="MAENKIEVSKLNLYYTDFHALKNVDINIAKNTVTAFIGPSGCGK
                     STFLRTLNRMNDLIDGVRISGKISLDGADIQKYDVIELRKRVGMVFQKPNPFPMSVYD
                     NVAYGPRVHGLRDRRKLDDIVEKSLKSAAIWDEVKDRLKKSALGLSGGQQQRLCIARV
                     LAVEPEVLLMDEPTSALDPGSTLKIEDLMEQLKKDYTVVIVTHNMQQAGRISDYTAFF
                     LNGEIVECDLTENVFYKPRDKRTEDYITGRFG"
     gene            19931..20587
                     /gene="phoU"
                     /locus_tag="PKI50_05325"
     CDS             19931..20587
                     /gene="phoU"
                     /locus_tag="PKI50_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNT03166.1"
                     /translation="MTRNYFDQELQELQVSLLKMSSMVEEILGNSVKALKAQDAELAQ
                     KVIDDDDEIDTMEYVVEDKCIKLIALQSPLARDLRVIATALKIITDLERIADYAVDIA
                     KITIKLQHEKYIKELIDIPRMAEIAVRMVKGAIDAYVHLDTSQAHEIAKLDDEVDGLY
                     KQIFRELLFIMMEDPRTVHQATYFLLISRYLERICDHVTNICERIIHTVTSEHVNLNE
                     "
     gene            20684..22027
                     /locus_tag="PKI50_05330"
     CDS             20684..22027
                     /locus_tag="PKI50_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNT03167.1"
                     /translation="MGLYVREENNLANPGVVIMEVRPSSIAEELGIKKGDRLISINGA
                     RIKDILEYKYLTADEFLEVEIEHGNGEIWIYEVEKEYDEDIGIIFEGIIDRPKSCHNK
                     CIFCFIDQLPSGMRETLYFKDDDTRLSFLQGNFVTLTNLSEREIDRIIKYHISPINVS
                     VHTTDPGLRRKMLNNKNAGKLMDYLERLKEGKIEVKAQIVLCPGVNDGENLLKTLEDL
                     AGLHPQVSCVAVVPVGITKYRQGLYELAEYDGISAASVIRMVRKAQDKYLSELNTRFV
                     FLSDEFFLISGEELPGFEEYEEFTQLENGVGLIALFNREVEESLKKLGRYNKKTRLIS
                     IITGEYAAPNLKMAASKIESCIEGLKIRIFPINNDFFGPGVKVAGLLTGKDIMDQLKD
                     KKVGRDIFIPDCMLKKDEKIFLDDVTVEEMEKELGAKITICREDGSDLVKKIISV"
     gene            22055..23368
                     /gene="der"
                     /locus_tag="PKI50_05335"
     CDS             22055..23368
                     /gene="der"
                     /locus_tag="PKI50_05335"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNT03168.1"
                     /translation="MAKSIVAIVGRPNVGKSTLFNKLAGKRISIVEDHPGVTRDRIYT
                     EVQWQDRAFTLIDTGGIEPHSEDLMLKQMKQQAEVAIETAEVIVFLVDGQEGITPSDR
                     EVANLLRRTRKKIILAVNKIDAPKYKDNIYEFYNLGLGEPMGISSGQALGLGDLLDEI
                     INNLLPEEEEVYDEYTIKVAVVGKPNVGKSSLVNKILGEQRVIVSDVPGTTRDAIDTP
                     FTVGEDKYVFIDTAGMRKRGKVFESIERYSVIRSLTAIERADVCLMLIDGKEGVSEQD
                     SKIAGYIHDQGKAAVIIVNKWDIVEKDDKTIDKYKADIRNELSFMEYAPILFVSALTG
                     QRVNKVLELVNHVSNQHAMRISTGMLNDVINEAVLTNQPTVSGGRRFKIYYATQVSIK
                     PPAFALFVNEPSLMHFSYQRYLENQIRKAFSFEGTPVRFLLRKKE"
     gene            23671..24699
                     /locus_tag="PKI50_05340"
     CDS             23671..24699
                     /locus_tag="PKI50_05340"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNT03169.1"
                     /translation="MSNNISIIGAGGWGTAVSVLLSGKGFNVKLWARDNELIEKMRDT
                     RENPAYLPGVTLKENILVSSDIEYCCSGSEIIIIAAPSHAMRDTCKKIKEFASKKQII
                     VSLSKGIENITLQRMSEIINEIMTENEVAVVSGPSHAEEVARGIPTAVVASSPKRAVA
                     EYIQDIFMSPLFRVYTNPDIIGVELGGALKNIIALGAGIIDGLELGDNTKAAVMTRGI
                     VEMARLGESLGACRSTFNGLSGIGDLIVTCTSMHSRNRRAGIAIGQGKSVEEVIRGTR
                     MVVEGIRTTKSAYLLAQRQKVEMPITQEIYNLLFNKTDIRNSVMNLTMRSKTHEMEEV
                     AENKVFEW"
     gene            24826..26304
                     /gene="spoIVA"
                     /locus_tag="PKI50_05345"
     CDS             24826..26304
                     /gene="spoIVA"
                     /locus_tag="PKI50_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HNT03170.1"
                     /translation="MEGYDVYQDISERTEGCIYIGVVGPVRTGKSTFIKRFMDLLVLP
                     NMDNEFKRERTRDELPQSANGKTIMTTEPKFVPNEPIELTLRENAKFKVRLVDCVGYL
                     VEGAMGHLEDNSPRMVNTPWFEDRIPFEEAAEIGTRKVIADHSTIGLVVTTDGSITDL
                     PRRSYIEAEERVIKELKELEKPFIVALNSARPKAQETIELREALETKYNTPVMIIDAL
                     KMEMEDLEGILGKILFEFPAREINFHIPRWMDTLELDHWLKQGIVDSIRTNLSEVNRI
                     RDIDDAIRKFDGAENLQAVEISDMRLGEGVVNITISLDDGLFYRVLGEVSGYSLEGDY
                     QLVALVKELAQSKREYDKVKSALGDVKEFGYGMVAPSVEEIKLEEPVIVKQGSKFGVR
                     LRASAPSLHIIKTDIETEIAPIVGTEKQSEEFIKYFMEEFENNPQKIWESNMFGKSLY
                     DMVKEELQGKLYKMPEDIQNKLQLTLQKVVNDTRGSLICIII"
     gene            26463..27146
                     /locus_tag="PKI50_05350"
     CDS             26463..27146
                     /locus_tag="PKI50_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HNT03171.1"
                     /translation="MMESIKRLRNPSELKIAAKENLDGKWLIAIAVTVVAWLLLDAFT
                     SSNGGNAAYKYIIENGKSIRVQADGSAFKNMMSIVSLILGGPIYFGVASYFLKLARYE
                     TAEFNDMFSGFSLFKTNFVLHLLMIVFTVLWALLLIVPGIIAAIKYSMAFYILNDNPE
                     IGAMEAIRRSKEMMEGHKMRFAEMWLSFLGWFLLGIVTFGLGMIYAVPYYRAAKANFY
                     LDLKESYSV"
     gene            complement(27187..27339)
                     /locus_tag="PKI50_05355"
     CDS             complement(27187..27339)
                     /locus_tag="PKI50_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03172.1"
                     /translation="MKHSKGVNAASSTGAVDESRNETTRIPYTDSISAPASDNIQKKH
                     KKNKKK"
     gene            27580..28512
                     /locus_tag="PKI50_05360"
     CDS             27580..28512
                     /locus_tag="PKI50_05360"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HNT03173.1"
                     /translation="MRCRRNEREKGDCSGRGAVYSLVNLIFGLVALTIKMTFKIVEVV
                     FQTLFGFISLFVSGSRDSQKTPNVYEKPMPEKNEAPSNVREFKKPESSKAQADVPEVE
                     SSDAPEEVKNKDFYVVLFILTIIPVIVALAMGKLYYAGIIGAAGFGLMMLVGIAASAY
                     GKGAAKETCEDEEEAEEDDSVERLIKDAFEKLFTIRKDIGKIQDADIRARLEAICCTG
                     EKIIGEVRTNPEGLAHVRKFFYYYIDAFGEIVNKYLKLSSFEESSEEIGRLMAETERS
                     FADIDGIFKDLCEKLLEKDMMHLKAEINVIKNSN"
     regulatory      28566..28696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            28830..29921
                     /gene="ribD"
                     /locus_tag="PKI50_05365"
     CDS             28830..29921
                     /gene="ribD"
                     /locus_tag="PKI50_05365"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNT03174.1"
                     /translation="MKRALELAARGSGRTSPNPLVGAVIVKNGRIIGEGWHERAGEPH
                     AEINAIMNSRESVEGATIYVNLEPCSHFGKTPPCAEELVKRKFKRVVMAMEDPNPLVA
                     GRGIKLLRESGIQADVGVLNAEALKLNDIFIKHITVKEPFVLLKTAMTLDGKAASRTG
                     DSKWISGESSRAYVHSLRNRYSSILVGVNTVISDDPELTSRIDGVQTRNPVRIIMDSG
                     GRIPIDANVLNIDESRRTIIAATEDMTEEKRRQLESKGAEILITDKRNGRVDIRQLLE
                     ELAGRGIDSLMIEGGGTVAAAFLEEGLIDKVAFFIAPVVIGGKTAPTPLMGEGVSLIE
                     KGYRLKHRSISVFGQDVLIEGYINVPWER"
     gene            29928..30572
                     /locus_tag="PKI50_05370"
     CDS             29928..30572
                     /locus_tag="PKI50_05370"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNT03175.1"
                     /translation="MFTGIVEEIGRIRNIRKGEKSESLIVECKNILEGTKMGDSISVN
                     GVCLTVADMSANSFEADVMPETVRATNLKYLKPGDSVNLERALSPTGRFGGHIVSGHV
                     DGTGTIQEIKREDNAIWFTISARNEILKYVIHKGSVAVDGISLTVQYADERVFKIALI
                     PHTRDYTTLPGRKVGYCVNIECDIIGKYIEKLMNYTGKDSKGGISLEYLLENGF"
     gene            30584..31780
                     /locus_tag="PKI50_05375"
     CDS             30584..31780
                     /locus_tag="PKI50_05375"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNT03176.1"
                     /translation="MFCRIEDALDELKKGNMIIVVDDEDRENEGDLLMAAEKVTPEAI
                     NFMAMHGRGLICVPMTEERLKELKIEQMTGNSTDLRETAFTVSVDIKSAATGISAYER
                     ADTVRALVNPDTKPEELAKPGHIFPLKARKGGVLKRAGHTEAAVDLAGMAGFYPAGVI
                     CEIMKDDGTMARLFDLFEYAKKHKLKMITIADLIKYRSREEKLVYKAADALLPTKYGE
                     FRVMAYESKINGEHHIALVKGDVAAEEPVLVRVHSECLTGDAFGSVRCDCGEQLAEAA
                     AMIQDEGRGVLLYMRQEGRGIGLVNKIKAYELQDTGIDTVEANELLGFPADLRDYGIG
                     AQILKDLGIKKIRLITNNPKKIKGLAGYGLEVVERVPIEMDANIRNEKYLLTKKEKMG
                     HMFHNI"
     gene            31777..32259
                     /gene="ribE"
                     /locus_tag="PKI50_05380"
     CDS             31777..32259
                     /gene="ribE"
                     /locus_tag="PKI50_05380"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNT03177.1"
                     /translation="MRGGKKLRVYEGNLTAGDRRFAVIAGRFNEFISSKLLGGCIDGL
                     KRHGCPESNIEVSWVPGAFEIPLAAQKLARTGRYDAVICLGAVIRGSTPHFDYVCSEV
                     SKGIAKVMLDTGLPIIFGVLTTESIEQAVERAGTKSGNKGFDAAMTAIEMANLLSTIG
                     "
     gene            complement(32871..33986)
                     /locus_tag="PKI50_05385"
     CDS             complement(32871..33986)
                     /locus_tag="PKI50_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008910026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT03178.1"
                     /translation="MKDPRIKQMAINLLNYSVQLKPNENILIEVFDSGEELASELVKE
                     IYKIGGKPFLTIKNRSLMRELILGTDAEHMSRTAHYEAERMKDMQAYIGIRGAYNSSE
                     WADIPSEKLGFYQSFWSKTVHTNIRVAKTKWCIMRFPNSSMAQMANMSKEQFEDFYFD
                     VCNLDYSKMSKAMDPLIELMNNTDRVRIIGTGTDLSFSIKGLPAIKCDGKSNIPDGEV
                     YSAPVRDSVNGYITYNTPSEYQGYTYENIRLEFKDGRIINASANDTDRINQVFDTDEG
                     ARYVGEFALGVNPYIIKPMKDILFDEKIMGSFHFTPGSAYDHCFNGNNSAIHWDLVNI
                     QTPEYGGGEIWFDDILVRKDGRFILPELDVLNPENLK"
     gene            34257..34958
                     /locus_tag="PKI50_05390"
     CDS             34257..34958
                     /locus_tag="PKI50_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNT03179.1"
                     /translation="MSVIEHAYGKTFKTILWAVNPLKKVFKKTLCEVHVFINEKAVEI
                     LRNDGFQDAYEFFSINKGCLNDGAVWADQDFKSREHFYNPFTHRGLYGCKSSKHLFRK
                     YYGCALVYWDCGDKDKAMFYLGAAVHLVQDATIPQHGSVKLLKSHRKYEQWIHKVHDD
                     FLHYSVSEGGIYLENPFEYIEKNAKEALEAYTKYSLIRNPSEKFFRIANRTFPLAQKT
                     TAGCLMNFYAKINEG"
     gene            complement(34945..36714)
                     /locus_tag="PKI50_05395"
     CDS             complement(34945..36714)
                     /locus_tag="PKI50_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNT03180.1"
                     /translation="MKNGILLCAINAKYIHSNLAVRYLKKFCEAHIKGIDIAEFSIND
                     SISSILKKLYCSNARIYGFSCYIWNISMVLDICSSLKKARPEAVIILGGPEVSYDAAD
                     LLSKYSFIEYIVIGEGEAAMLDLLCFLTAGKSNLPDIPGIAFRSGESVAITEPRPDIP
                     DLDMLPFPYDSFDGLDNRIIYYETSRGCPFNCQYCLSSTIRGVRYLAMDRVRQDIKSF
                     ANAGIKQVKLVDRTFNCDLERSIEIMQYIIDLNPSTNFHFEIAADLIDVRFLEIAGKA
                     PDGMFQFEIGVQSTNPKTLSEIKRKMDFDKVSHNVARLLSFKNAHIHLDLIAGLPYED
                     MESFEKSFNDVYSLNPDMLQLGFLKLLKGSGIRQGCKKYRIQHHDFPPYEVISTAWLS
                     FKEIMELKDIENVLEQYYNSGRFRHTLDFLLQAAETEPFDFYRRLSGYWNQKGHFNAP
                     KNVNELYTVLKCFAEDAFVNRLDNEQFGLLNEYMKLDWLLYVRSGSMPAAINRFNHAA
                     IKEEIHGYIKNKLTDMDTFKDFKNLSLREILKQVGYEVFLRNIFADTPSQDEIVMFFP
                     LRPSSSKKIINFAAVPLKEFTLH"
     gene            36862..37506
                     /gene="nth"
                     /locus_tag="PKI50_05400"
     CDS             36862..37506
                     /gene="nth"
                     /locus_tag="PKI50_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNT03181.1"
                     /translation="MRDNDDLKSRAKKISEILFKIYPEATCSLDYDKPLELLIATMLA
                     AQCTDERVNIVTRDLFKKYTSVYNYASANPEELEEDIRPTGFYRNKANNIIGCCKKLI
                     KDFGGKVPDNMEDLLSLSGVGRKTANVVLGNIYKIPGIIVDTHCKRLSNRIGLTKEED
                     PEKIEFDLMEILPRENWTAFSNSLVYHGRAICNARKPKCVQCHIAQYCDFFNNI"
     gene            37712..38038
                     /locus_tag="PKI50_05405"
     CDS             37712..38038
                     /locus_tag="PKI50_05405"
                     /inference="COORDINATES: protein motif:HMM:NF028309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HNT03182.1"
                     /translation="MTTDIIKGIRETELAAEEKIKAAQQKAKDILMSAEQEADHIIKA
                     AVDQAIQESKKQLEVAEKEANLEAESKRKQNDELCQELRRKAEEKMEEAVNLVMERIV
                     NMNGNS"
     gene            38025..39941
                     /locus_tag="PKI50_05410"
     CDS             38025..39941
                     /locus_tag="PKI50_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HNT03183.1"
                     /translation="MAIVKMKKATMIALQSEKNEILNDLQRFGYLQVVNLEERMEQGF
                     EELQPDSDNEAVGRLEAKLSQVKYSIDFLGKFNTAKKPLFAQKVQVDETKRKEYLDNE
                     EKLNEIYESCRSIDSRFTEIKSSETKLNNAIAQLTPWLALKEKLEEIGTTHNTNSILG
                     FIASKYEDELSTAVKDEDLTAYLEKVGVEKENSYFMVIYHQTCEERISQLLKQVGWTK
                     VTFSEYSGTPEENINRIKAEISNLENIKKELAKKAEAMVPESGFLEILYDLLSIEKDR
                     RSVVKDFIKTDKTFMLQGWIPEKFADDMTKLLDGITDKYTISFEAPNEEDEIPVLLNN
                     PKPVKPYEMITDLYSIPNAKGIDPNIFMAPFFVIFFGIMVTDAVYGIFMSAVTGYVLY
                     KYKPQGGMKSMMWIMFFGGISTFFWGGIFGGWLGDLIKVKPWWFNPLDEPLKMLIFCL
                     ILGVIHLYTGYILQAYQNIKAGNFMDAIYDQVLWLMLLTGLMFLALPPLAEIGKYMAI
                     LGAAGTVIFAARSEKNIFKRFTSGVLALYGVSGFLGDVLSYSRLFALCLATGVIAQVF
                     NAMGVMMGGSIIGKIIMVAFLAFAHVFNTALGVLGAYVHTSRLQYVEFFGKFYEGEGK
                     PFNPLRIKTKYIQE"
     gene            40006..40479
                     /locus_tag="PKI50_05415"
     CDS             40006..40479
                     /locus_tag="PKI50_05415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNT03184.1"
                     /translation="MGNSGLYLAFLGSAIAAGLAGCGSAIGVGIAGQAAAGVITEDPS
                     KFGKTLLLQALPGTQGIYGLLIGFLIWSKIGVFGTILPVTIEQGLMLMLAGIPIGIVG
                     LISAIYQGKASAASCGIIAKRPEEFGKGMIYPAMVETYAVLSLLASFLMLNGIKL"
     gene            40638..41240
                     /locus_tag="PKI50_05420"
     CDS             40638..41240
                     /locus_tag="PKI50_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNT03185.1"
                     /translation="MAGVENIKEKILQDADLRAKEILEKARLQANEIVEKAKQKAASR
                     AKEISQKSVYDVSEKKRIINSIVELEMRKDVLAEKQKAIGEVFDKVLERMNNLEDSRY
                     EKVIIDMLAASAESGEEEIVFSESGRKKLSSDFLNKLNKTLAAAGKKGNLKISDETRN
                     ISGGFILKSQGVEINNSFEAIIRLYRDEIEPKVAEIFFRA"
     gene            41286..42287
                     /locus_tag="PKI50_05425"
     CDS             41286..42287
                     /locus_tag="PKI50_05425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HNT03186.1"
                     /translation="MSENKYLYSVTRIRALETKLLDKAKIERMIEARNADEIIKILYE
                     TEYANSISEMKSVEDYESVLSKELSNTYKLLSEISPVPELTNLFQLRYDIHNLKTLLK
                     SSYLDEENDELLSEIGTIPIQQLKGMIKEKVFSDLDPLVRESLEEVVGEFTVNPDPQL
                     IDVTLDKALYKLMYKAAKDNKSSFLTDYISTQIDLINIKSLIRVKSMGYGREFLKKVI
                     LENGKLDYAFFSDIFDESLETLIDRLAYKDYGKVVEEGISNYIKTKSLTKFEKLSDDF
                     IFELAKKGKFVAFGIEPLIGYLMAKENETKIIRMIMVGKINEIPNELIRERLRDVYV"
     gene            42280..42603
                     /locus_tag="PKI50_05430"
     CDS             42280..42603
                     /locus_tag="PKI50_05430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNT03187.1"
                     /translation="MYKIGVIGDKDSILGFKALGISVFPTNDIREANRLIHKLARENY
                     AVIFVTEQLAKDLDEAIGEYKNSLTPAIIMIPNNRGTLGLGIDGVKKSVEKAIGSDIL
                     FGKEG"
     gene            42608..44380
                     /locus_tag="PKI50_05435"
     CDS             42608..44380
                     /locus_tag="PKI50_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNT03188.1"
                     /translation="MSDGRIIKVSGPLVIAEGMLDAKMYDVVRVSDKNLIGEIIEIRG
                     DKASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLIESIFDGIQRPLDVVRSASGDHI
                     ARGVQVEKLNKTRKWNFEPSVKQGDAVKTGDIIGIVKETSIVDHKIMVPHGIEGTIEE
                     IYSGEFTILETVARIKTKDGIKEVQMLQKWPVRKGRPYKEKLSPSTPMTTGQRIIDTF
                     FPVAKGGTACIPGPFGSGKTVVQHQLAKWADAEIVVYIGCGERGNEMTDVLMEFPELK
                     DPKTGEPLMKRTVLIANTSDMPVAAREASIYTGITIGEYFRDMGYSVALMADSTSRWA
                     EALREMSGRLEEMPGEEGYPAYLGSRAAEFYERAGKVVCVGTEDREGALTAVGAVSPP
                     GGDLSEPVTQATLRIVKVFWGLDAALAYRRHFPAINWLQSYSLYLDRLGDWFTEEVAL
                     DWNNLRMETMRLLQEEAELNEIVRLVGFDALSLKDRFTLEASKSIREDYLHQNAFHEI
                     DTYTSIHKQYRMLKAILLFYHEGQKALSEGAFYKELTALPVREDIAKSKLILENDIES
                     FDVLEANIRDQIASILSKGGKIVC"
     gene            44374..45753
                     /locus_tag="PKI50_05440"
     CDS             44374..45753
                     /locus_tag="PKI50_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNT03189.1"
                     /translation="MLKEYKTIKEVAGPLMFVDQVEGVKYDELVEIELKNGEMRRGKV
                     LEVSGSSALVQLFEGSTGININEGKVRFTGHSLELPVSTDMLGRVFDGLGRPKDGGPK
                     IIPESRKDINGNPINPAARDYPSEFIQTGISAIDGLNTLVRGQKLPVFSGSGLPHARL
                     AAQIARQAKVLGTESKFAVVFAAIGITFEDADYFVSDFKRTGAIDRAVLFMNLANDPA
                     IERIATPRMALTAAEYLAYEKDMHVLVIMTDITNYCDALREVSAARKEVPGRRGYPGY
                     LYTDLATIYERAGRIRDRKGSITQIPILSMPEDDKTHPIPDLTGYITEGQIILSRELY
                     RKGVMPPIDVLPSLSRLKDKGIGRGKTREDHADTMNQLFASYARGKEAKELAVILGEG
                     ALSDTDKLFAKFADEFENKYVSQGDSEDRSIEQTLTLGWELLSILPKAELKRIKDEYI
                     DKYLPKGDA"
     gene            45757..46407
                     /locus_tag="PKI50_05445"
     CDS             45757..46407
                     /locus_tag="PKI50_05445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNT03190.1"
                     /translation="MAKLNVNPTRMELTGLKGRLKTAKRGHKLLKDKQDELMKKFIDY
                     VKRNMELRLDVEKELTAAFKNFLIARAVMSSEVLEEAIMYPTQKINLEMSTKNIMSVN
                     SPVFNLVKEENKEASSIYPYGFAATSGELDGAIFSLQEVFDKLIELAQVEKTCQLMAD
                     EIEKTRRRVNALEYVMIPQLIETVKYITMKLDENERGSRVRLMKVKDMMEKQKAEA"
     gene            complement(46441..46647)
                     /locus_tag="PKI50_05450"
     CDS             complement(46441..46647)
                     /locus_tag="PKI50_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HNT03191.1"
                     /translation="MHKNISSNDISSNKMHAHNHSIKCNVDTCSYYDNNYCSASVIEV
                     NPMGDGVAHSSEGTCCTTFVEAKQ"
     gene            46920..48098
                     /locus_tag="PKI50_05455"
     CDS             46920..48098
                     /locus_tag="PKI50_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT03192.1"
                     /translation="MGIQFANRMDNLKGSVIRELLKYAEQPDIISFAGGLPAPELFPV
                     EEMMKATDRVMMEDGRASLQYGATDGYLPLRKKIAERSKKIGFVPDPSEILITSGSQQ
                     GLDFTAKIFINKDDVIITESPSYLGALNAFKAYQPKFVEIPMDEDGMIIEELEKALAA
                     NKNVKFIYTIPDFQNPTGRTMPVERRKKLMELATEYEIPVIEDNPYGELRFEGEILPS
                     LKCFDPKNLVILLGTFSKILAPGMRLGWVIARPDLLIKYNLVKQGADLQSSSMAQRQA
                     NMFMEMYDIEKHIEKIIKVYSARRNLMLDTIKKEFPSNVKCTNPQGGLFAWATFPEEV
                     DASVILKKALEQKVAFVPGEPFYPNGGNANHCRLNYSCMSEDKIVEGIARLGKTLKSM
                     "
     gene            48308..48466
                     /locus_tag="PKI50_05460"
     CDS             48308..48466
                     /locus_tag="PKI50_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNT03193.1"
                     /translation="MEKWTCTACGYVYDPEAGDPDNGIEPGTPFEDIPEDWVCPLCGV
                     GKDMFEKE"
     gene            48613..48686
                     /locus_tag="PKI50_05465"
     tRNA            48613..48686
                     /locus_tag="PKI50_05465"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48645..48647,aa:Gln,seq:ctg)
BASE COUNT        15752 a         8230 c        11731 g        12973 t
ORIGIN      
        1 ggacatgggg acagggacat ggggacgttt cgcgtgtccc actggaagtg ggacacgcga
       61 aacgtccccg tgtccctgtc cccatgtccc tgccgtcccc gtgtcctcaa aggaaaaaat
      121 aatcaccctg cccgtcacgc agggtgatta cgtttaacta atctcttcgt ggcaaccgca
      181 ctttacggtg tccggttacc ttataattat tatagcataa aaggggcaaa agtaaacata
      241 tttttgtaag gagtatccag tttatcatgg gatattgcat tatcacaaaa ttattttatt
      301 actgaaaacg catttcttca agaaataacc ttccgaataa tatattttca ataattgttt
      361 ctttgaaata agggtttggc atctcgcagc tgcttcccga tttttgaagg aaatacagct
      421 cttcactttc tcccatgacc tccgcataaa gcctgccgtc tttataaagc ttgtcgccta
      481 tcctcagatc tatatctctg aagcttatct gttcgaagcc tcttcccttg caattacttt
      541 ttctcttttt tttcatctga tacacctgct taaaggatac aatttattgt gccacattca
      601 tattttcgac atttttatca taagaattta taccgatatg caactcggct gataaaaatg
      661 tatatttatc gaattatatt gacaaaaaat aacaatgaat gtataataca tagcaataat
      721 agcaaaaaaa ggaataaata gaaagaaaaa acataaaagg aaagagataa tagaaatggt
      781 aattatgcca tgtatggggg atatgaatga gattgctttc agctctatac catgagggta
      841 tagaagaata tatcgaatcc attccgggga ttgaagtatt agacgttgtc ggaaataaaa
      901 gatccttgct tgattcatta agcaaggata aatacgattt ggtgcttatc tccaaaagcc
      961 ttcccgggaa tgaggaagcg gatttattga tagaaatgct tgcctccgaa gagttcaaag
     1021 gcctaagaac agcatatttg tacggagagt atgatggtta ttgtgatgat ttcataaagt
     1081 ttctgataaa tcatgggata tatgacttcc atgtcggtga tgaaataacc tccaaggata
     1141 ttgaaaggat cattttcaaa cctgcaagca aggagagggc actttactat tttagaagcc
     1201 attatgacaa tgaaatgaat atacattacc cggaacagaa aaataaaagg aataaacgct
     1261 tctttgaggg aagattcctg ttcaattccg gatttggctc attgttcagc aaaaatcata
     1321 agaaaaatcc taaggagaaa ctgattattt ctataatatc aaatcaggct accggcaaat
     1381 cccatacggc ttggaacctg agccattgtt tttccaaact gggatatgcc acaagccttt
     1441 ttaatattga cagaggttat tcggcaaatt tgttttttga tattgatgag gtttattata
     1501 atctgctgga ttttaccctt cagaacgaca tacacaaaga tatacttgaa aattgctata
     1561 acagaaaaaa tctttgcgtt gttaccggta aactgggtga tgagaaagaa atcagcagtg
     1621 aagacttcct gaagctgctt tactgcatac gtacaaaatc ggatataaca atcattgata
     1681 caaggacagg catatcagag ctgacaaggc tttcaatgca gaactctatt tatgacatgc
     1741 ttgtttttga ctgtgacatt atgcattttc atatgaatat gaaaatgctg gaggagttga
     1801 aggatgattt tgtacccgaa aagacaattg ccgtgattaa taatacaaat atacggtctt
     1861 caagccataa gttcatatat aatgaactga caagctcggg tatacccttt aaagatatgg
     1921 cattcgttag cagctgcggt ttcatcagtt atgaagtgat gcacaccggc tatacgccgt
     1981 accaggctgc ggctgacaga aataaaagct ttttaggtga tatggacagt ctgctggata
     2041 agctgggtat ttccaacgag taagaaggtg ataagcattg gcatacagta agaaaaacaa
     2101 ggtttttgca gtaaaaagaa cagccctgat atcaggctgc gttgtttgtg cagttcttat
     2161 cttatcagca gtttatatgc tttacggtaa tgtattttca ggagaagcgg caggaactgt
     2221 caggaacagc agtgaaaacg aagcaaaaac cgtggtgatt gccagggcaa aggagaattt
     2281 ggggcagggg gagctgcttg ctgcaagcat ggcggaatta atagagatac ctgttgaatt
     2341 ggcacctgaa ggagcaatta aaagcctttc aaagttgaat aacatgagat tgaaaagagg
     2401 aattgcgaaa aatgagtttt ttaatgatat ggacttgatg ccccaaaatg ctttttatga
     2461 aaaaggtgag aggctgatag aacataattt tgccgagggg gctgttcctg cctcggttac
     2521 agcgggaagc ataatagaca tcaagctttt tgtaaagggc ggagaggatg gcatagtcgt
     2581 ttcaaaggct gcagtaatct cgcgcagcgg taatttgttg tccttttacc tggacggaat
     2641 ggagcaggag ttcatcaaag aagcggctgc agaaggagcg ctctttgctg ttcagtatat
     2701 cgatgcttcg cagcaggcaa gtgaagttac atatactcca tcatatagca aggataaaca
     2761 gaactgattg gaagtgatat acatgcgcgg cgcaaaagga aagcttattt gcacctggtc
     2821 tccgatgctg catggggaag ggtgctccac cttagcctgt ggaattggaa tcggactgca
     2881 gtacctatca gggaagaaag tgctgattgt caataagagc aattctgcca gccacatgga
     2941 aagatacgtg gagaaggata ttgagattaa atactcaatg gacaatttaa agatattcaa
     3001 caaagaaata aagacagagc atattttcac ctatgcaacc cgtataaata aggatttata
     3061 catgatagcc ggctccaggc tgaacaggga tataatcggg gagaacagcg aatttgacaa
     3121 gatcttttta gaaaagtgcc tggcaggttt tgaccttgta attactgatc ttgatacagg
     3181 agtcagggat gacaacagga cgtacctgga tcaggccgac aaaattttag cggttgttac
     3241 accaaatgaa attataattg ataaattgta tgatgagtat agggtacggg atgctctcgg
     3301 ttattttacg gatgataagg cagtggtaat aattaataag ctctgcgagg gttgggatac
     3361 tgccggtgta atcggcagat acaagaacag gtactcctta tctgacattt ttggactgaa
     3421 ttatgacggt gatgtcttaa atgcctgctg caccgacaag aatttctact cctttcttat
     3481 gaaggaggcg aagcgtgaaa agaatatatt cataaagcag ctgagtgaga tctgccgctt
     3541 tcttatcgaa gggctgtgca tgggaaataa tgctgcggac agaattaagc atgggaatat
     3601 ttttaagaag tttttaaaga gcagcattta ctgacggggg gagtaaagca ttggacttag
     3661 gaaaatacct tattattgat tcattatgcc tgcatatgga aaagtacatc aacagcttca
     3721 ttaattcgga accctcagat gaacaggaaa aaagcatagt atcgctgctc agggattaca
     3781 aggaaaaatc cacctccggc cttccggagg caagggaagt tattaagtca catataaaga
     3841 ccggtattct tacaggcttt gatatatatg tgtccaacga gatgggcaga ctggaggatg
     3901 cgattatgtt gaaaggggta tgcgttgatg aagcaaatgg acttgccgat ttgatattac
     3961 ccttcaacga accgcaaaag cttacttcga gagagaaatt cgagataatt ctgcataaga
     4021 acaggaattc atccggcaac ggaagggacg gagccttcaa ggccattctc gcaaggtatt
     4081 catgctgctg caaggccagg gaggcagcag gttacaggag cttaaggtat gaatatgatg
     4141 aagcggatat tgactatatt tataataatg aagattatgt gctttctttc agcgataaga
     4201 tagaaattat cacgcaaagg ctgtatgaag agatattcgg actgaaacac attgacatgc
     4261 tggcttattc ggatattaat gaagttggtt tttcaaataa tggaaggtat gtctactgct
     4321 ggtccggaaa aaagatatgg ctgagctttc tgcagataag tgagaaggat gcaagggtga
     4381 tacaggacag ggctatcagc tttgaaaagc actgccccca gcttgatgta agccatccgg
     4441 agatactatg ccacagaggt gacggggcta gaataacagt tacccaaaag ccgtattttt
     4501 cggcgcgcaa cctatgcata aggattttta atcaaagctg tgccgatttc aatgacctga
     4561 taaccgcgga taagctgagg acattgatta ttgctctggt taagaccgga gagagcatct
     4621 gccttcaggg gggattaggc acgggaaaga ctactacaat gaatgtaatg tatgagctgc
     4681 tggatgatct tctgcatatc ggaacaatag aggactattt tgagcagcat attatggaaa
     4741 aatatccata caagcgcatt gttgaaggac aggcagtcaa cggcaaggat cttctggacg
     4801 tggtcaagac aatgctgcgg atgtccgtgg atgtggccaa tatcggagag gtcagagacg
     4861 gaaatgcatt gttcgcattt attcagttgg tgcagtctgt ctcagtcgca gcatggttta
     4921 ccacgcatat aacaaatccc gagactacgg tgccgcggct gaaaaatatg cttatcggca
     4981 caggcaggta cttttccgag cagagcgcaa taatggatat tgtacattat attaatatga
     5041 tttttcagca tgaaataata gatgggcgga taacaataac aaaggctgtg gaaattgttc
     5101 ccctggtggc ggtatcaagt gacggtgaat atgatattga gactcccaga gagcagcttg
     5161 agaagctgtt ttatattcaa cagctgcgga gcaatacata taatatgttc aggttgaata
     5221 ccataatgca gtcggttgat gggaaggcaa aattcgtcaa ctatccttca agaaggatgg
     5281 tgaaaaaagc aaaaggggac agacaatcat gggagtattt aaaaaacctt atccgattga
     5341 ttgagaagga tacgggcatg gcaataccgg aatatgcaag ggggtgactt gattaatgcg
     5401 gaattattac agtaaaggca ttttccatgc tctgtttgca gggctgaaca atcgccttac
     5461 ccgtataaac cagaaatcgc ccgctcaaaa cgcaaggaca agcctggcac tacttaccgt
     5521 tttgacaatt cttgtcatta tatattttct gcttgcatgc agcactttca agggcctttg
     5581 gtatcataga gtgctgtgtg gtttttccgc ctttatattt atgtatttgt gcaaagaatc
     5641 gtattttgag atatatgaga agcggattaa aagggacttg cccaatacct taaagaagct
     5701 tactcattac tacaatcatt acaggggaaa tatcattccg gcattggaag atactgtttc
     5761 cagatgcccc aggagcaata gaatctatat gatagaaatc agggaagcac tgatgaagcc
     5821 ggactatcaa gtacagatag aggaactgga gggcaaaatg cctatgattt ggctgaaaat
     5881 gctgtgccgg cttattcttt ccgcaaagga gaatggaggt atgtcctcgg agggagacac
     5941 cgttgcaaat aatctgaagc gaatgaccaa tataattaca tttcttaata ttgaacaagg
     6001 gtataatgat gccgagcttc tgggaatgca gatttttgtg ttttttactc cttttttagt
     6061 gataccattg acaaaatggt acaatatcag tttgctttca ggccttaaca ttggggacat
     6121 atatggaagc atacaagccc aggggcttac tgcaattatg atgtttatca gcggggtcgg
     6181 aacaatattc attcattgga tgagaaagct tcaaaactga ttaaggggtt gagcaacatt
     6241 cagtatttca tattgattca agctattgca attgctttgc tgatagctgc agcttctata
     6301 tttgtccatc agtatttcag gtataggaaa atcaaaaggt tatatagggc tgattatgcc
     6361 aggaaaagct gcttgaagag catttccagg ttcctgattt ttatgccggg atataaaagc
     6421 agaagataca aaaatacaga gggattgata ataaactcag gattgagggt ttcagtcgag
     6481 gaattttatt tggcaaagac attgcttttt gtcctcggct ttgtgttttt tatcagtgtg
     6541 caaactacaa atacttttgc cttctacgac catgttatca gcgatttgaa catgggaaat
     6601 acattgatag atgaagctgc accaaatgat tatacattgc agcttgaaaa agaggttttc
     6661 attcacatag acagcagcct gcccaagggg aaagcggccc tgaaggaact taaggatgaa
     6721 aacaattccc aggtttacat cggatatatt gaggctcaga taagcagtaa atgggctgga
     6781 ctggaagagt atgcagggga aatggctgag aggatgtata agaaactaat aaaaatcaga
     6841 aggatagaaa cagactacct gatttattta acttcattta tactggcaat aatactgtat
     6901 tttatcccaa acataatcgg acatttaaaa ctaatactta tagaggacaa aagggactgg
     6961 gaaatactga actatattta tgtattttca atgtttgggc gaatgccgcc ctttaatata
     7021 aaaaatgtgc tttccaatat tttggtgatt tcagatatct ataaaccaat aataaatgaa
     7081 gccttgaata atttaaacag cggaaaaggg gaagaggcct ttgacaacct gctgcaaaaa
     7141 gtggaaaacc aggagcttta tgaactcctt gagtccatgc gcctttctat gagcacaggg
     7201 cttctcaaca tagtggacaa cattgatgaa atggcggtaa accagcttaa atggctggaa
     7261 ataaagagtt taaaaagaag aaagtcgaag caggtaatcg caatggttcc ggtagtactg
     7321 gtaatgcttg cggcagccgt atatttttcc tacagccttt cgacactttc aaatccaatg
     7381 aactatttaa aataaaatgg ggggagtatg aatcatggaa aaattctggg cggtaatatt
     7441 gatatttgtt gtggttgcgg cagcggggct gatgtatgtc aatcaggtgt atattcctca
     7501 gaaagagacg cagatggcaa tggcggatat ggcgaatcag actctatctg cagttaaagt
     7561 ggagataggg gatgatagtg ttcctcaaga ggttagtggg tctgaaataa aagatataat
     7621 agaggattac tcagatgatg atataaaggt ttacattaat ggaataattt atgatggtac
     7681 taatgtaatt gaacattcca gccagtacta tatgagagta ataaagaatg atggtgagat
     7741 aactgagatc agatttacga taattgtgta gttaaatgcg acggggacac ggggacacgg
     7801 ggacgtttcg cgtgtcccac tgaaagcggg acaggggaaa cgtccccgtg tccccgcgtc
     7861 cccatatccc cgccgcatta ttcttcagat gtgaaattat aactgcaagg agattatgca
     7921 aatggaaaaa ttctgggctt caatattggt tatcccgatt tttacatggt actccttcca
     7981 tgccgtcctg gacagggatt tcaggataaa gcagcaggca gtggagaata tagtatgtca
     8041 atatacacag atagctgcaa aaaaaggtgc cttgtataat tctgtctata atgagatgaa
     8101 agaaagatta tcaaggttcg gtacatttga tatcagtttg acggcagaga ggttcaccgg
     8161 cagcagcgat acgccggaac tgataaaagg ttcatatata ataggaaggg atttgcgctc
     8221 ggaaggtttt gacataataa acatatatgc tgagagcatt aaccgtcatc cccttggaag
     8281 gttgtatgaa ctcacacctt ttggaacaaa gtcaggtgcg gaagcagata tcaggtattt
     8341 cggcaaagca tcggtatata ttcaatagta taggaagtgg ctcgatgaag tcgttttttt
     8401 ggagtttgat ttttatattc tgcacatgga gcatgttctt catagggaag ggagtagaca
     8461 acatagacat tatggctgag aggtacaaga gggcactgga tgccggagca aatgcagctt
     8521 ccggatacag ggcctattat tctgagaaaa tgctttataa tcatggtaca ggattcggca
     8581 ttggccttga ggacagcaat aatttgccgg tagataggga agaggctgtt aaatggtttt
     8641 accggttgtt tttcaggaac cttggtatag aggacaccaa taagcagaat gagtataaga
     8701 ggtatatgcc tatgaaagcc ataatccttt ttgacaggct tatgatagct gatgcggatg
     8761 acaactggca tacatatgat cctgcagggg aaaaagaata catgatccaa tacagaggaa
     8821 aggattataa gttcacattg tctgatcagg tctatgacat tgaaaacagc tcgtggatta
     8881 tggcagatga tatagggctg gaggcaaaag aaagaaaaga gctggtcacg aattacataa
     8941 tcaatgagct taacagcttt ttggataaca gaggcaataa ggaaagcggc agttattaca
     9001 agatagtatt ctctctggac gatgcttcag acagtaagct cagcggaata aacgggatta
     9061 acttttttat actatgcgaa ggaataccca taccatcact taatcccttt aaacaggaaa
     9121 agttttttgc ttacagcgta ggcggaagcg agataaaaaa gaagcagttg tgatgataca
     9181 gatgttaacg actcccccac ctgaaatgcc aacgcatttt ttaggtgggg ggcctttttg
     9241 cactatacta tgtattaata tgcagaaaaa tgtcgaatta ttactatatt tgtattactg
     9301 ttattattgg aaatttcaaa cttttgtaag ataatatata tgctggcata aatccgggtg
     9361 tcatgggaat tattattgac tgtaagatta ataaataaat tttcttatgg gggagctgaa
     9421 aatcttgagt gttctgcctt acattactgc cgtagacaat agagtggagg atgtaaataa
     9481 accttttctt gatcttacgg gatatacgga aagtagtatc cggggaagaa atgtagagga
     9541 gatatggcag cagcttctaa gaataagtat ggacttggac agtgtaaagt gcttgaagga
     9601 atgtagctgc tatatgtttg acatcctgcg cagggcaaag gaagtgacta taagctatac
     9661 tgcggacttt gagctgaaac aatgtattta tactttcaaa cggaaagtga attccatgct
     9721 tgaggacaaa tttccatttc ttgagcatgc aaattccgcc aatgatacgg gagtagccct
     9781 ttacagtgtg ccggaattca tactcctgaa agggaatgag agtcataaca ggtttttaag
     9841 caggctgctc ggcggtgcgg aagacattat cggaaagcgg attgaggaga ttctgcccga
     9901 ttggaaggga agcgatgcgg aaaatatatt cagcaatgtt gtaaagacag gcaaaccaat
     9961 attctatgat gaacatatga gggaacatcc gtcaacaggc attgtctgta cgagatatac
    10021 tcttaccccg atttttgagg gagaagagtt aagatacctt gtgcttattc acaatgacgt
    10081 tactaaaaat ctgacaatag agagagaaaa agattatttt gaaaagcaga gggattacct
    10141 gtacaggctg ttcaatgcca tgaatctgcc aatcatatcg ttttcatacc ccgaatatag
    10201 gattaaggaa tttaacaata aagcattggt tgagttgagc gaactgacag gcatggataa
    10261 tttgactatt gacccgagta tttcttgcaa aggcctggct gaaatgatac cactttttga
    10321 tattagcagt gcaaagatat taattgaaca gatgaagcaa accaaatctc ctgtgtgcca
    10381 taaaaaaatg gaactatgta aaaaaggccg caaggtatat tatgatgtta cttttcatcc
    10441 ttttttgaac ataatggagg aaatttcaga gatattgata gttgctgcgg atgttaccga
    10501 cgaagtggaa aaagcgaagc atatagagaa tatattgaag cttaaggatg aattcttata
    10561 ttttattact catgagttta agactccgtt atcggttata aatgcggcag tacagacctt
    10621 ggagcattgt tactcaatgc agattcctga caaagccagg tttttaattg gaaaggttaa
    10681 acaaaattca tacaggcagc ttagacttgt aaacaatctg ctggagattg cacgtataaa
    10741 ttcgggctat atcaagctca gacaaaggaa tgtggatata gtttttctga caagagctat
    10801 tactgagtct gtggcaattt atgcccagca aaaaggcatc gaattggttt ttgttacaaa
    10861 attcccgcaa agagtaattg ggctggatga tgaaaaactt gagcgcgttc ttcttaacct
    10921 gctttccaat gcaattaaat ttacacccgc aggcggaaag gtagtggtgg aactgtcctc
    10981 aaagctgcat aagcacaaaa gggttatctg tatcaaggta aaggatcagg gtgtcggaat
    11041 ccctaaggaa aaacagtctg ttatatttga tcgtttcgga caagtagaca atttgcttac
    11101 cgtccaggct gagggcagcg ggatagggct gtatcttgtc aagcttatgg tggattccat
    11161 tgggggagaa ataactttgg agagtgaatg gggtaagggc agtgtgttta ctttaatgat
    11221 accctcaaaa aaatcaaagg agaatgccaa agagcagaaa ttcaagctca gatccgacaa
    11281 tcggcttata caaaccacag ctattgagtt ttcagatata tacttataag gagtcaattc
    11341 gaatcaattg catcggaagg cagcgttata ttttcaacaa tgtattggaa tatgcccatt
    11401 ggagtttcca ccttaacatc atccttgact ttcttcagaa ggaatgccct gcccataggc
    11461 gacagatatg acgcactatc gatattaaga tctgcttcgc ccataaaagg agatacaatc
    11521 tgcatttcct cgattttgtt tgtataacaa tccttcagaa taacctgact tccgattatt
    11581 gcaaaagggc aagtatcgga tgcattgtcg tccacaagaa cctcattgta tataatgttt
    11641 tcaacaccct taatataaga atccaggagt tcctgaaaat cattcctttc atttgtccac
    11701 acaggataat atttatcaag tacatgaagc ttttcttctt ctattctaac caggtgatta
    11761 acaagcattt catatatttt ttttgatata atagattttg acaccattaa tacctcctaa
    11821 atattacatt aaatggtaaa taaaaatata atacaacaag gcaccttgca gcaaatctgc
    11881 aaaatgcctg taaatacagt ttaacataaa tgtaaatcga tgtaaatagt ggattgcatt
    11941 tccatttaat tgaaatgctg gatttttcgc acaaaatatt cttaattgct caaaatacat
    12001 cttgacagct attaagatat agattattat tatatcatac caaaatggta ggaattaaga
    12061 aaaggctttg aacggaaagt gtaggcacac aaatatttct tcagagaaag gctgcatcgg
    12121 attgctccga ttgctgaaag cagccttagg gattggtgct gaaagtgcgt ccgggagctt
    12181 ttgaccgaac aagctaaaac aaaggtttaa gtaggatata acgggagcac ccgttacagt
    12241 gcagggatat gttagtatcc ttaaaaagta ataaaagagt ggaaccgcac tttatgcctc
    12301 tttatacata tgtatgtatg tgtaaagggg cttttgtttc cataaaccaa aaatctatag
    12361 taaaagggtg gtaataatgt ttggacgact gaaggaagac ataaggacaa tatttgaaag
    12421 agatcctgct gcaaaaagtt atcttgaggt tatattgtgt tatccgggcc tgcatgcggt
    12481 tatttgccac agaattgctc acggcttgta tcgcaggagg cggtatgtcg cagcaaggct
    12541 gatttcgcaa ataagcagat tctttaccgg aatagaaata caccccggtg ctgcgatagg
    12601 aagaaggttt tttatcgatc acggttcagg aatagttata ggcgagacaa ctgaaatagg
    12661 tgacgatgtg accttgtatc agggggttac tttgggaggc acgggaaaag ataagggaaa
    12721 gaggcaccct accataggga ataatgtaac aatcggctca ggagctaaaa ttcttggacc
    12781 aatatcaata ggacataatg caaaaattgg agccggtgca gtagtggtga gatgcatacc
    12841 tccgaacagc acggctgtcg gtgtacctgc tcatgtggta ggaagggaag gaatagacga
    12901 gacctatgtt gatttgaacc atacggatat tcctgatccc cttgaagatg aaatcaggga
    12961 actggagaag agaatcaggg aattggaacg aaaacttttt tctattgatt aaagctcaga
    13021 ataatattaa cataacatta gcagtaatta ttatattcag aggtgataga aataaaagaa
    13081 gtatttaaaa cagttatgaa agacggatta gcgtatctta caatacccgc ctttgaaaaa
    13141 accggcatgg taaagcactg cttcactaca aggataggcg gtgtaagccg gggcatatac
    13201 aattcactta atacctccga tgataaagag gatcctgtgg aaaacgttca taggaacctg
    13261 aaattggtgt gcggagctat aggtattgat tatagcaatc tggtgttctc tgatcagacc
    13321 cacgaggata atataagagt tgtttcaatt gcagatttag gaaaaggtat atccgtaccg
    13381 aatgatataa agaatacaga cgggttaatg acaaatatac ccgggatacc cttaataacc
    13441 ttttatgctg actgtgtacc gcttttcttt ctcgataaag aaaataaagc aatcgcagtt
    13501 gtgcattcgg gctggaaggg tacggttcaa aaaataggag taaagacaat aaggcagatg
    13561 acagaggtat atggaacaaa accggaggat tgcctggcgg ggatagggcc ttcaataggc
    13621 ccggagtgtt ttgaggttgg acaggaggtt gcggatcagt ttactgaaag ctttggcacc
    13681 cggcctcata tcataaagcc gttagggaac gggaaataca tggttgacct gtgggcagcc
    13741 aataaactga tgcttatgga aatgggagtg ccggagaata atattacaat cagcggatta
    13801 tgcacaaaat gcagggaaga tttgtttttt tcatatagga gggacaaagg cagaacagga
    13861 agcatgtcgg caataatgga actgaaggag gaagatacct gatgaaccgg aagatactta
    13921 ttatagagga tgaagcaaat attcaggaac ttcttaggta taacctggaa aagaacgggt
    13981 atattgttac tatttcagat aatggcattg acggacttca gaaagctctt gaccaagtgc
    14041 ctgacctgat actgcttgac cttatgctgc ccggacttga cggtttggag gtatgcaaga
    14101 ggttaagaat gcaaaaagca acaagaagga ttcctatatt catgctaaca gcaaagagtg
    14161 aagagctgga caagatattg ggcctggagc tgggagctga tgactatatt acaaagcctt
    14221 tcagcataaa ggaactcatt gcgagaataa gagctgcaat gagacggttg gatgatgaaa
    14281 gcagcgctgt gtctgataaa gcagagaaaa ataaaatact caaagcaagg gatatagaaa
    14341 ttgacagcga gaaatatgaa gtaaggaaaa acaatgaaaa aattacactg acccttaaag
    14401 aattcgagct tttgaaaatg cttgtagaga ataagggtaa ggttttgaca agggattttt
    14461 tgttggataa aatatgggga tatgaatata tgggtgatac aagaactgtt gatgttcata
    14521 tcaggcatgt aaggcagaag cttaatgatg atgaaggcag cggccggatg attgagacta
    14581 taaggggtgt aggctataga ttcataagtg aaggtgaata ataatgcagc gaaaaatttt
    14641 ttttaatatt gtcgttgtac ttcttctggg agtgatcatt tcaggtttat tgtctgcaag
    14701 aatagtgaaa aaaaaccttc tcagtgatat tgagaaaagt cttacaacag aagccgctct
    14761 tgtaagggag cttctggggg aaagcttaat aaacaacagc aacgatatag gcatttatgt
    14821 cggtaagata aaacagactg ccaactcgcg aataacaata gtagatgtta tgggcaatgt
    14881 aattattgat accgataagg aatactctga aatggaaaat catgccggaa gacctgagat
    14941 tgcagcagct ttaaagggcg aggcagaaag ttctataagg tatagcagta ctcttagagt
    15001 ggattttata tatgtggcac agcctatttt gaaaggaagc agcataattg gagccgtgag
    15061 gctgtcgaaa ccgctctatg aagtaaaaaa tatgcttaat agtttgtata tcaatgtctt
    15121 cattgcagta ctaataggta tttttgtagc ttcagtgctt ggatataaaa tggcgatgaa
    15181 tataaccaag ccgattaagg agattacgta taccgcatca agaattgcaa aagggcagtt
    15241 agacagacgg attaacatta caagccggaa tgaaataggt atattggcag attccataaa
    15301 tgacatggca tcaaagctga gggacactat aaccagcctg caggataaga atataaagct
    15361 tgaatctata atgtccagta tgataaatgg aataatagca atagacaatt cagaaaaaat
    15421 actatttata aaccatgtgg cagagagcct cctggggatt accggcggag aaatcacagg
    15481 aaagcatctc cttcaggttg taaggaacaa ttccattgat aattacttta aaaaaatgtt
    15541 aaaggataaa gagtttttta atacagaggt gaatcttaat gactccaatg aaaagatatt
    15601 gaagtttcat gccaattcta taaagcagaa tggtgaaaat ggtattgaag gaattattat
    15661 aaccatacag gacgtaactg agcttagaaa actggaaaga gtcaggacgg aatttattgc
    15721 caatgtttct catgaactca aaaccccttt aacatccata aaggggttta cagagacact
    15781 taaatcattt gattttgatg ataaacagga tgcaataagg tttttaaata taattgagga
    15841 tgaagcagac aggctttaca ggcttattaa cgatatactg tccttatccg agttggaaca
    15901 gaaaaaaaca aagactgtta aagaaaaaat agatatagaa aaagctgtag aagaagtgct
    15961 gtcagtactg aaaagccaga gtgacaaaaa gaatatagaa ctgtcagcga agattcagga
    16021 ggggcttgaa agtatttcag gcgatgggga caagttcaag cagatgctga taaacctgat
    16081 agacaatgca gtaaaatata ctcctgaaaa tgggaaagtg agtatagagg catataagca
    16141 atcacataat gctgatttag acaagctgat tataaaagta aaagataatg gtattggaat
    16201 acccaagcag catataccaa ggctttttga aagattctac agggtggaca aagcaagatc
    16261 aagaacaata ggaggcaccg gccttggcct tgcaatagtg aagcatatag taatgctttt
    16321 caatggtgag atagaagtaa ccagtgaagt gggaaaaggc actgagttca gaataatact
    16381 gcctatgaaa tgataaaaac tgcaattttg cagttttttt ttacatggaa ttaacaattg
    16441 cttaatattt cttttaccat acctttatat attgttaaca gtactgttgt atattctaat
    16501 tgtgataaga aaaaaataat acgatgaagg ttaaggagga tttattagga tgagaattaa
    16561 gaaaacactt gcattcattt gcgcagcagt attgcttata ggtgctttaa caggatgtgc
    16621 gccggcagaa acaaatgaac cggcagctaa tggtttgggt gaaagtataa ctataaatgg
    16681 atcaacatcg gtgcagccgc tggctgagga acttgcaaaa gcatttaaag aaaaaaatcc
    16741 cgatgcaacg atagacatac agggcggcgg ctcaggtgta ggaataaaat cggctgctga
    16801 cggagttgca gatatcggaa tgtcttcgag ggacctcaag cccgaagaaa aatcattaaa
    16861 ggaatttaaa atagctgttg atggtatagc agtaatagta aatcccacaa atgaaataca
    16921 aggcctttca atggagcaga tactcaagat atacaccgga gaaatcacag attggaagga
    16981 agtaggaggg aaagaaggga aaataacggt tgtaaccaga gaagaaggtt ccggtacaag
    17041 aggagcattt atagagctaa ccaaagttga aagcaagtca gaaggcgaca ggactgttgc
    17101 ttcagcaata acacagggtt ccacaggtgc ggttattaca acggtagcgg gggatcccaa
    17161 tgccataggc tatgcttcct tcggatcggc aaaagaaaat acggacataa agctgataag
    17221 tgtggacgga aagacatgta ctgaagaaaa catatatgcc ggagaataca agatatcgag
    17281 accttttctg atgctcacac aggcagaacc gacagggctt gccaaagcat tcctggattt
    17341 catactaagc gaagaaggac aaacagttgt aagtgggcat aattatctta aggctgtgaa
    17401 ataagacgga aaaggggagc aaatatgaag gcttatgaac gggtgataca actggtactt
    17461 tttatcagcg ccataatagc cgtgtttgga gttatactga tttcatactt tataatcagc
    17521 gagggatatc ctgtgattcg caaggtcgga ttattggatt tcctttcagg taatagatgg
    17581 gcgccaacac aaggagtcta tgggatatat cctatgattg taggatcatt gcttgttaca
    17641 gcaggggcat tggtgctggg agtgccgata ggaataggct gtgcaatatt cctggcagag
    17701 ctggcgaagc ctagaatagc aggaatcctc aggcccggaa ttgaattgct ggcgggaata
    17761 ccttcagtta tttatggatt gtatggactg gcagtaattg ttcctttcat cagaaaagtg
    17821 ttcaataatc agggcttcag cgttctggca gggagcatag tattggcagt aatgattctg
    17881 cccacaataa taaatgtttc ggaaagtgcc ataagatcag tccccaggga ttataaggaa
    17941 gcatccttgt ctttgggttc ctcccactgg cagtccataa aaaatgtaat agttcctgct
    18001 gcacgttcag ggattactgc cgcagtcata cttgggatgg gcagagctat tggagaaact
    18061 atggcagtaa taatgatagt cggcaacagt ccgttagttc ctgaaagcat attgtcaccg
    18121 gtcaggacac ttacaggcaa tataggcatt gaaatgggat atgcaggagg agaacatcag
    18181 aaggcacttt ttgcaaccgg tattgtactc tttgttttca taatgctgct taatatagct
    18241 gcacacctta tcccaaagaa ggcaggtgaa tgaattgacg gaatcgagca ttacttcagg
    18301 aagtgtaaat aaaatcagaa agaatgaaaa gacagccttt atatttcttt gggcagctgc
    18361 agctataacg gttttgactc tgatagctat tgtggggagc gtgctgataa acggaatagg
    18421 tcatttgagc ttggatttcc tgactaagga gcctgaaaac atgggcaagg acggaggcat
    18481 attttcaata ataataacaa ccatatatct ggcattattt tctctgttaa ttgcagcacc
    18541 tataggcata agcgcagcaa tattccttac tgaatatgcc aaagagggcc ctgtcataag
    18601 gctgataagg tttggaaccg agacactggc aggaattcct tccataattt ttggcctttt
    18661 tgggtatgtt ttctttgttg tgttcctgaa attcaggtat tccatggtat ccggagggct
    18721 tacacttaca tttatgatac ttcctacaat aataagaaca gcagaagagt ctataaaaat
    18781 ggttccaatg tcttataggg aaggaagtct tgcattggga gcaacaaaat ggcagacaat
    18841 atataaggtg gttctgcctg catcaattcc cgggattctt accggagtca tattgggcat
    18901 aggcagggtg gtaggagaga cagcagcagt gatatacacg gcaggaagct cacttggact
    18961 gcccaattca atatggaggc ccgggaggac tatggcggta cacctttata tattggcatc
    19021 cgaaggatta tcaaagagta atatgtatgc aactgcaact gtattaataa taactgtgct
    19081 tataataaac tttacagcca acaggctgat aaagcaattt ataaggtcat agcagagggg
    19141 gatgaaaatg gcagaaaaca agattgaggt cagcaaactg aacttgtact atacagattt
    19201 ccatgctctg aaaaatgttg atataaatat tgcaaaaaat accgtcacgg ccttcatagg
    19261 tccctcgggc tgcggtaaat ccacattttt gaggaccctt aacaggatga atgatcttat
    19321 agacggtgta agaatcagtg gaaaaatttc tcttgatggt gctgatattc aaaaatatga
    19381 tgtgatagaa ttaagaaaaa gagtaggtat ggtatttcag aagcccaatc cttttcccat
    19441 gtcagtttat gataatgttg catacgggcc cagggttcac gggcttagag acaggagaaa
    19501 gctggatgac atagttgaaa agagtcttaa aagtgctgca atatgggatg aagtaaaaga
    19561 caggctaaaa aagtcagcat tggggctatc agggggacag cagcaaaggc tgtgtattgc
    19621 cagggttctt gcggtagaac ctgaggtact tcttatggat gagcctacct cagcacttga
    19681 tccgggttca acccttaaga ttgaggattt gatggagcag cttaaaaagg actatacagt
    19741 agtaatcgta acacacaaca tgcagcaggc gggccgtatt tcagactata ctgctttttt
    19801 cctcaatgga gagatagtgg aatgtgattt gacggagaac gtgttctata aaccgagaga
    19861 taaaagaaca gaggattata taacaggaag attcggataa acagtgagta attgaaatgg
    19921 aggtgtgagg atgacacgta attattttga ccaggaattg caggagctgc aggtatcgct
    19981 cctgaaaatg agcagtatgg ttgaggagat acttggaaac tccgtaaagg ctcttaaggc
    20041 tcaggatgca gagttggcgc agaaggtcat tgatgatgat gatgaaatag ataccatgga
    20101 gtatgtagtg gaggacaagt gtatcaagct catagcactg caatcccccc ttgccaggga
    20161 tttaagggta attgccacag cacttaagat aattacagac cttgagagaa tagcagatta
    20221 tgcagtggat attgcaaaaa taacaattaa gcttcagcat gaaaagtata taaaagagct
    20281 tatagatata cccagaatgg cagaaattgc agtaagaatg gtaaaagggg ctatagatgc
    20341 atatgtacat ctggatactt cccaagccca tgaaattgca aagcttgatg atgaggtaga
    20401 cgggctgtat aaacaaattt tcagagagct gttgtttatt atgatggagg atcccaggac
    20461 cgttcaccaa gctacttatt tccttcttat aagcaggtat cttgaaagaa tatgcgatca
    20521 tgtgacaaat atatgtgagc gcataattca tacagtaaca agtgaacatg tgaatttaaa
    20581 tgagtaaaag gatatttcgg caaagtattc ttttattgtt atacccctat agtataatat
    20641 gaagctgata tgatataata ctatgtataa ttattggcga atgatgggtt tgtatgtaag
    20701 ggaggaaaac aatttggcga atccgggcgt agtaataatg gaagtacgtc cttcaagcat
    20761 agcagaggaa ttgggcataa aaaaaggtga caggctcata agcataaacg gcgcaagaat
    20821 aaaagacatt cttgaatata agtatctcac tgcagatgag tttcttgagg tggaaataga
    20881 gcacggcaat ggcgaaatat ggatttatga ggtagaaaaa gagtatgatg aggatatagg
    20941 aataatcttt gagggaatta ttgacagacc aaagtcttgt cataacaagt gtattttttg
    21001 ttttattgac caactgccct ccgggatgag agaaacctta tatttcaagg atgatgatac
    21061 aaggttgtct ttcctgcagg gtaattttgt gactcttacc aaccttagtg aaagagagat
    21121 agacaggata atcaagtatc acataagccc tataaacgta tcggtgcata caactgatcc
    21181 cggacttagg agaaaaatgc tgaacaacaa gaatgcagga aagctgatgg attatctcga
    21241 aaggcttaag gaagggaaaa tagaggttaa ggcacaaata gtgctttgcc cgggagtgaa
    21301 tgacggagaa aatctcttaa agactttaga ggatcttgcc ggactgcatc cccaggttag
    21361 ctgtgttgct gttgtacctg taggtataac caaatacagg caaggcctat atgagcttgc
    21421 ggaatatgat gggatatcag ccgcttcggt aataaggatg gtcagaaaag cgcaggataa
    21481 gtacctgtcc gaactgaata caagatttgt atttctgtct gatgaatttt tcctcatatc
    21541 aggggaggaa ctgcccgggt ttgaggagta tgaagaattt acgcagcttg aaaacggagt
    21601 aggccttatt gcacttttta acagagaggt tgaagagtcc ctgaaaaagc ttggaagata
    21661 taataagaaa acgaggctga tttctataat aacaggagaa tacgccgcac caaatcttaa
    21721 aatggcggca tcaaaaattg aaagctgtat agaaggcctc aaaatacgta ttttcccaat
    21781 aaacaacgac tttttcgggc ccggtgtgaa ggtagccggc ctgcttacag gaaaggacat
    21841 tatggatcag cttaaggata agaaagtcgg aagggatata ttcatacctg attgcatgct
    21901 gaaaaaggat gagaaaattt ttcttgatga tgttactgtt gaagagatgg aaaaagagct
    21961 tggagctaaa atcacaatat gcagggaaga cggcagtgac cttgttaaga aaataatatc
    22021 ggtttaatgg attagtgtca ggaggtgagg catattggca aagtcaattg ttgcaatagt
    22081 aggacgtccc aatgtcggaa agtcaactct ttttaacaaa ttggcaggta agaggatttc
    22141 catagttgaa gatcatcccg gtgtaaccag ggacaggatt tatacagagg tccaatggca
    22201 ggacagggca tttacattaa ttgatacagg cgggattgag ccgcatagtg aggatttgat
    22261 gctgaagcag atgaagcagc aggctgaagt tgctatagaa actgctgagg ttatcgtatt
    22321 ccttgtagac ggacaggaag gtataacccc ttccgaccgg gaggtggcca atttactacg
    22381 aaggacccgt aagaagataa tactggcagt taataagata gatgcaccga agtataagga
    22441 caatatatac gaattctata atctgggatt gggggaacca atggggattt cttcggggca
    22501 ggctttggga ttgggggatc tgctggatga aataatcaat aaccttttac cggaggaaga
    22561 agaggtatat gatgaataca caataaaggt tgctgttgtg gggaagccca atgtcggcaa
    22621 gtcttccctt gtaaataaga tactcggaga acaaagggtt atagtcagtg atgttccggg
    22681 caccacaagg gatgccattg atactccctt taccgtagga gaagacaagt atgtatttat
    22741 tgatactgca ggaatgcgaa aaagaggcaa ggtgtttgaa tcaatagaga gatacagcgt
    22801 tatcaggtct ctaactgcca ttgaaagggc agatgtgtgc cttatgttaa tagacggaaa
    22861 agagggcgta agcgagcagg acagtaagat tgcaggttat attcacgatc agggaaaggc
    22921 tgctgtaata atagtaaaca agtgggatat agtggaaaag gatgataaga ccatagataa
    22981 atataaagcg gatataagaa atgaactttc ctttatggag tatgcaccca ttctatttgt
    23041 ctctgctttg acagggcaga gggtgaacaa ggttctggag ctggttaacc atgtatccaa
    23101 ccagcatgcc atgagaattt caacaggtat gctgaatgat gtgataaacg aagctgtgct
    23161 gactaatcag cctactgtct caggaggaag aaggtttaag atttactatg ccacacaggt
    23221 ttcaataaaa ccgccggcct ttgcactttt tgtaaatgag ccctccctga tgcatttttc
    23281 atatcaaagg tatctggaga atcaaatcag aaaagccttc agctttgaag ggactccggt
    23341 gaggttttta ttgaggaaga aggagtagaa aaaagggggg tgctttttat agatgaaata
    23401 tgtatttgtg agtataattc aggatccgca aatgtcttta gggtattggg cgcaaagggc
    23461 ggaaaaggca ttgcaacaac aattggactg atgctgccca tgatccttta atggtagtcc
    23521 tcatatttat tgttggtgtt atagttatta tcactaccag atatgtttct ccggcatcaa
    23581 tagtgggagt gacaatatat cccatagcaa tgataatgac acggaatcca aatttggaca
    23641 aaagaagata acagagcagg agggtaaagg atgagcaaca atatttcaat aattggtgcc
    23701 ggcggctggg ggacagccgt atcggtgctg ctttcaggaa aagggtttaa tgttaagctt
    23761 tgggcaaggg acaatgaatt gattgaaaaa atgagggaca caagagaaaa tcctgcttat
    23821 ctgccgggtg tgacattaaa ggaaaatatt cttgtatcct ctgacattga atactgctgc
    23881 agcgggagtg aaataatcat tatcgctgct ccttcccatg caatgaggga tacgtgcaaa
    23941 aaaataaagg aatttgcatc aaagaagcag attattgtca gcctgtcaaa gggcattgaa
    24001 aacattacac tgcaaagaat gtccgagatt ataaatgaaa tcatgactga aaacgaagtg
    24061 gctgtagttt cgggccccag ccatgcggaa gaagtggcca gaggcattcc taccgccgta
    24121 gttgcatcat ctccaaaaag agcagtggct gagtatatac aggatatatt tatgagtcct
    24181 ctattcagag tatatacaaa tccggatatt atcggggttg aactgggcgg ggcattaaaa
    24241 aacataattg ccctgggtgc ggggattatt gacgggcttg aattgggtga caatacaaaa
    24301 gcagcggtta tgaccagggg catagttgag atggcaaggc tgggagagtc cctgggggct
    24361 tgcaggagca ctttcaacgg cttgtccggt ataggagacc tgattgttac ttgtacaagc
    24421 atgcacagca ggaacagaag ggcaggaata gccatcggac agggcaaatc tgtggaagaa
    24481 gtgattagag gtacaagaat ggtggtggag ggtatcagga ccaccaaatc cgcatatctt
    24541 ctggcacaga gacagaaggt ggagatgccg attacccagg aaatatataa cctgcttttt
    24601 aataaaacgg atataaggaa ttcggtaatg aacctgacca tgagatccaa gacccatgag
    24661 atggaagaag tagctgaaaa caaagtattt gaatggtagg tatatccctt tgaagctcga
    24721 agattgctta gcaatcttct ttttttgtaa taataattgc acatgcacat atatatttag
    24781 agaaggacta tgcaattatt tgaacattat taaggaggga agagtgtgga aggatacgat
    24841 gtttatcaag atatatcaga gagaaccgaa ggctgcattt acataggagt tgtgggaccg
    24901 gtcagaacag gcaaatcaac atttataaag agatttatgg atttgcttgt gcttcctaat
    24961 atggacaatg agttcaaaag agaaagaacc cgggatgagc ttccccagag tgccaacggc
    25021 aagacaatta tgacaaccga acctaaattt gtgcctaatg aacctattga gctgacttta
    25081 agagagaatg cgaaatttaa agtcaggctg gtagattgtg taggatatct tgtggaaggt
    25141 gcaatgggcc atttggagga taacagccca aggatggtta atactccatg gtttgaagac
    25201 cgcataccct ttgaagaggc ggcggagatc ggaacaagaa aggttattgc ggatcattcc
    25261 acaataggcc ttgttgtgac taccgacgga tccataactg atcttcctag aaggagttat
    25321 atagaagcgg aggaaagagt cattaaagaa ctgaaggagc ttgaaaaacc atttatagtg
    25381 gctctgaatt ccgcaaggcc gaaggcacag gagacgattg aactcagaga agctcttgag
    25441 acgaaatata atactccggt catgataata gatgccttga aaatggaaat ggaggatttg
    25501 gaagggattc tggggaaaat actctttgaa tttcctgcca gggagataaa cttccatata
    25561 ccgcgctgga tggatacgct ggagctggat cattggctta aacagggcat agttgacagc
    25621 atcagaacga atctcagtga agtaaacaga ataagagata ttgacgatgc cataagaaag
    25681 tttgacggtg cagaaaacct tcaggctgtg gaaatatctg atatgcgcct tggagaagga
    25741 gtcgtgaata taaccataag cctggacgac ggacttttct atagggtttt gggagaggtt
    25801 tcgggctata gcctggaagg agattatcag cttgtcgcat tggtaaagga gctagcccaa
    25861 tcaaagcgcg aatatgacaa agtcaagtca gccttggggg atgtcaagga attcggttat
    25921 ggcatggtag ctccttccgt ggaggaaata aagcttgaag agcctgtaat tgtgaagcaa
    25981 ggcagcaaat tcggagtaag gctgagagcc agtgctccat cgctgcatat aataaaaacc
    26041 gacatagaaa ctgaaattgc tccgatagtt ggaacagaga aacaaagcga agaattcatc
    26101 aagtacttta tggaggagtt tgagaataat ccgcagaaaa tatgggaatc caatatgttc
    26161 ggaaagtctt tgtacgatat ggtcaaagag gaactgcagg gcaagctgta caagatgccg
    26221 gaggatattc agaataagct gcagcttaca ctgcagaagg ttgtcaatga cacccggggc
    26281 agtttgatat gcataatcat atagaggagt aaaacaaaat aagggtgttc tgattgattg
    26341 caaaccagga caccattttt tttaaaaatt tttgaaactt ttttgtgcat ttaacagtat
    26401 ttagatatag tgaatattta agaaaataat atttgtgata aatttcaaga gggggttata
    26461 atatgatgga aagtataaaa agacttagaa atccttcgga gcttaagatt gccgccaagg
    26521 aaaatcttga tggaaagtgg cttatagcca ttgctgtaac tgttgtcgca tggctgctgc
    26581 tggatgcgtt tacatcaagc aacggaggga atgccgctta taagtatata atagagaacg
    26641 gaaaatctat aagggttcaa gccgatggaa gtgcatttaa aaatatgatg tcaattgtaa
    26701 gcttgatatt gggcggaccg atttatttcg gagttgcttc ttattttcta aagcttgcga
    26761 gatatgaaac ggctgagttt aatgacatgt tttcaggctt cagcttgttt aagactaatt
    26821 ttgtcctgca tttactgatg atagttttca cagtattatg ggcattgctg cttattgtac
    26881 cgggcataat agctgcaata aaatactcaa tggccttcta tatattgaat gacaatccgg
    26941 aaatcggagc tatggaagcc atcagaagaa gcaaagagat gatggaagga cataagatga
    27001 gattcgccga gatgtggctc agctttctcg gatggttttt attgggcatt gtaacctttg
    27061 gattgggaat gatatatgca gttccttatt acagggcagc taaagctaat ttttatctgg
    27121 acttgaaaga aagctacagt gtttaaaaga ccctataaca cattaaaagt aacaggcatg
    27181 agcctgtcac ttttttttgt ttttcttatg ctttttttgg atattgtcag aagcgggggc
    27241 cgatattgaa tcggtgtatg gaatcctggt ggtctcattt ctgctttcat ctacagcccc
    27301 ggtgctgctg gcggcattta ctccttttga atgcttcaaa gcatcaactc cttttatctg
    27361 atattcagat acactatatt ttgcagctat ttgcgaattt ttattcaaaa aggacacggg
    27421 gacgttcggg acaggagaaa cgtccccgtg tcccttgtga tattattttt tattttttga
    27481 aacaaaacga ggacaactgc agtatataat tgtgaagaaa gaaattcacc cggttcgtgg
    27541 tacggggtga attaatccaa ttgacgggag cgaattatta tgagatgcag aagaaatgag
    27601 agagagaagg gtgattgcag cggccgcggt gcagtttact ctttggtgaa ccttatattc
    27661 ggattggtag ctctcacaat taaaatgaca tttaaaattg ttgaagtagt atttcagact
    27721 ttattcggat ttatttcact ttttgtttcc ggatccagag attctcagaa aactccaaac
    27781 gtgtatgaaa agccgatgcc tgagaagaat gaagcaccca gcaatgtgag agaattcaag
    27841 aaaccggaaa gcagcaaggc acaagccgat gtcccggaag tcgaaagcag tgatgctcct
    27901 gaagaggtta agaacaagga tttttatgtg gtgttgttca tactcacaat tattcccgtt
    27961 atagtggctc tggcaatggg caagctatat tatgccggaa taataggggc agcaggcttt
    28021 ggacttatga tgctggttgg aattgcagcc tctgcatacg gaaagggtgc ggcaaaggaa
    28081 acttgtgagg atgaggaaga agctgaagag gatgattcgg tagagaggct gataaaggat
    28141 gcttttgaaa agctctttac gataaggaag gatattggaa agatacagga cgcagatata
    28201 agggcaaggc ttgaagccat atgctgtaca ggggagaaga taattgggga ggttaggaca
    28261 aatccggaag gacttgctca tgtcaggaag tttttctatt actatattga cgcttttggt
    28321 gaaatagtga ataagtatct gaagctgtca agctttgagg agtcttcgga agaaatcgga
    28381 agactgatgg ctgagacaga aagatccttt gccgatatag acgggatttt taaggatctg
    28441 tgcgaaaagc tgctggaaaa ggatatgatg catcttaagg cagagattaa tgtaatcaag
    28501 aacagcaatt agagttttga caaatatacc ggctgcagtt ataattataa aggaaatgaa
    28561 tataatgtaa tcttcaggga tgggtgaaat tccctaccgg cggtaaagcc cgcgagccgg
    28621 tggtgcctag tgcatcatca gcagattcgg tgagactccg aggccgacag taaagtctgg
    28681 atggaagaag gtgcaatctt tatctatttg tgcatatttt tgagccctga agtttttttg
    28741 cttcagggct tagttttttt ctttccaaat atggaataat aaccagtagt aaaaaaggag
    28801 gagtgattgg agtggaagat gcctcttaca tgaagagggc tcttgaattg gctgccagag
    28861 gcagcggaag aaccagccct aatccgcttg taggtgctgt cattgtaaaa aacggaagaa
    28921 ttatcggaga aggatggcat gaaagggcgg gagagcccca tgcagagata aatgccatta
    28981 tgaattcaag agaatctgtt gaaggagcca caatatatgt gaatttggaa ccttgctccc
    29041 acttcggaaa gacacccccc tgcgccgagg aacttgtgaa aaggaagttt aaacgtgtgg
    29101 ttatggcaat ggaagacccc aatcctttag tggcgggacg gggtataaag cttctcaggg
    29161 aaagcggaat tcaggccgat gtgggagttc tgaatgctga agccttgaaa ttgaatgata
    29221 ttttcataaa gcatataaca gtaaaggaac cctttgtgct tcttaagaca gcaatgactc
    29281 ttgacgggaa ggccgcctca aggacgggag actccaaatg gataagcggt gaatcctcaa
    29341 gggcatatgt ccacagctta aggaacagat attcatcaat actggtaggt gttaatacag
    29401 tcataagtga tgatcctgaa ctgacctcaa gaattgatgg agttcaaaca agaaatcccg
    29461 taaggataat tatggattca ggagggagaa taccgattga tgcaaatgta ctgaacatag
    29521 atgaaagcag gaggacgatt attgcagcca cagaggatat gacggaagaa aagcgcaggc
    29581 aattggagag caagggtgcg gaaatattaa ttactgataa aagaaatgga agggtggata
    29641 taaggcagct tttagaggaa cttgcgggta gaggtatcga cagtcttatg attgaaggag
    29701 gcggtacagt agcagccgca ttcctggaag aaggtttaat agacaaggtg gcttttttca
    29761 ttgcgccggt tgtaataggg ggcaaaactg caccgacacc gctgatgggg gaaggggtca
    29821 gccttattga aaagggttat aggctaaaac acagaagcat cagtgttttt ggacaggatg
    29881 tcctaattga gggatatata aatgtacctt gggagaggtg aattgacttg tttacgggaa
    29941 ttgtcgagga aataggaaga atacggaaca taagaaaggg ggaaaaatct gaaagtctta
    30001 ttgtcgaatg caaaaatatt cttgaaggaa ctaagatggg tgacagcatc agtgtaaacg
    30061 gcgtttgcct gactgtcgca gatatgtctg caaacagctt cgaagctgat gtgatgccgg
    30121 aaacagtgag agctacgaat ttaaaatatc ttaagcccgg ggattcagtc aaccttgaaa
    30181 gggcgctttc ccccaccgga aggttcggcg gccatatagt gtcggggcat gttgacggga
    30241 caggaacaat acaggaaata aaaagggagg ataatgcaat atggtttacc atttctgcaa
    30301 ggaacgaaat actgaaatac gtgatacata aaggttccgt tgcggtggac ggcataagcc
    30361 ttacggtaca atatgctgat gagagggtgt tcaaaatagc attgataccg catacccggg
    30421 attacaccac actgccgggc agaaaggtag gatactgcgt gaatattgaa tgcgacataa
    30481 taggcaaata tatagagaag ttgatgaatt acacagggaa ggacagcaag ggcggtattt
    30541 ccctggagta cctgctggaa aacggttttt aggaggaata gacatgtttt gcagaataga
    30601 agacgcttta gatgaattaa agaagggcaa tatgataatt gttgtggatg atgaggacag
    30661 ggaaaatgaa ggagatcttc tcatggcagc tgaaaaggta actccggaag caataaattt
    30721 catggctatg cacggaaggg ggcttatttg tgtccctatg accgaagaga ggcttaagga
    30781 gctgaaaata gaacaaatga caggaaacag caccgactta agagaaacag catttacagt
    30841 ttcagttgat atcaaaagtg ctgctaccgg aatatccgca tatgagaggg ctgatacggt
    30901 aagggcatta gtgaatcctg atacaaagcc ggaggaattg gccaagcccg ggcatatatt
    30961 tcctttaaag gcgagaaagg gaggcgtact taaaagagcc ggacatactg aggccgctgt
    31021 ggatcttgcc ggaatggcag gtttttatcc ggcaggggtt atatgtgaga taatgaagga
    31081 tgacggaaca atggcaaggc tttttgacct ttttgaatat gcaaagaaac acaagcttaa
    31141 gatgattact atagcggacc tgataaaata cagaagccgt gaggagaagc tggtatataa
    31201 agctgcagat gctttactgc ctactaaata tggggaattc agggtaatgg cttatgagag
    31261 caaaataaac ggtgagcatc atatagcact tgtcaaggga gatgtggccg ctgaggagcc
    31321 tgtattggta agggttcatt cggaatgcct gactggtgat gcctttggct ccgtaaggtg
    31381 cgactgtggt gagcagctgg cagaagcagc ggcaatgatt caggatgaag gcagaggggt
    31441 attgctgtac atgaggcagg aaggcagggg aatagggctt gttaacaaga taaaggccta
    31501 tgagcttcag gacacgggaa tagataccgt tgaggcaaat gagcttctgg gttttcccgc
    31561 agacttaagg gactacggca taggggccca gattcttaag gatctcggaa taaaaaagat
    31621 aaggctgatt acaaacaatc caaagaagat taagggatta gcaggatatg gacttgaagt
    31681 tgtggagcgg gttcctattg agatggatgc gaacataagg aatgagaaat acctgcttac
    31741 caaaaaggaa aaaatggggc atatgttcca taatatatga gaggaggcaa aaaattgaga
    31801 gtatatgaag gtaatctgac agccggggat agaaggtttg ctgttattgc aggaaggttc
    31861 aacgaattta taagcagcaa gctgcttggg ggttgtatag acggacttaa aagacacggc
    31921 tgccccgagt ccaatattga ggtaagctgg gttccgggtg cttttgagat accgctggcc
    31981 gcacaaaaac ttgcaaggac cggaaggtat gatgcggtga tatgtctcgg agcggttatc
    32041 aggggttcca cacctcactt tgattatgta tgcagcgaag tatccaaagg gatagccaag
    32101 gtaatgctgg atacaggatt gccgataatt tttggagttc ttacaactga aagcatcgaa
    32161 caggctgttg aaagggcagg aacgaaatcg gggaacaagg gttttgatgc agcaatgaca
    32221 gccattgaaa tggctaattt gctgagtacc ataggataag agtaatgaag tccacataaa
    32281 aatacagaga aataggactg ttgcacaata tcggaataat gcgacgcatc acaagagttt
    32341 gtaaagtact ccgactgtag gggaagtatg ttctcctcac tttccaaagc cagcaaaatc
    32401 actggcgggg gtcccatctc tgcgggcccg tctggttatt ttgccagggg tattcccgtt
    32461 ccgaggtcgg gtggaaagtt tcgtcaaaga tatcttcccc acgtcggtat ttgcatttcc
    32521 ttaatacagc ataattccca aagggcactg gccttgtata acatatgatg ctctgtagga
    32581 tgcatactaa gcaagagctt gtatagtata gcagggcaca cttttatgca ttttgtaaca
    32641 ggcctctgat atggctttgg caaaaagttc gtaaagccgg catggacgcc ggcttaggtg
    32701 ccacgggtca tggaggacct tttggcaccg gtagaacttt tccaaaggca tagcagtata
    32761 ggcctgtcaa aatcataatc cgtgtgcctc gatatactat actgtctctt gcggaaggtt
    32821 atgcaaactt cagatatgtc gcattcttct acttatgcgg cttattgaag ctatttcaag
    32881 ttttcgggat taagtacatc caattccgga agtatgaacc ttccgtcttt tcttacgagt
    32941 atgtcatcaa accagatttc tcctccgccg tattccgggg tctgtatgtt tacaagatcc
    33001 caatgaatgg cggaattatt accattgaag cagtggtcat aagcgctgcc gggagtgaaa
    33061 tggaagctgc ccatgatttt ttcgtcaaag aggatatcct tcatgggctt gattatatat
    33121 gggttaacac ccaatgcgaa ttctcccaca tatcttgcgc cttcatcggt gtcgaatacc
    33181 tgattgattc tgtcggtgtc atttgcagag gcattgatta ttcttccatc cttgaattcc
    33241 aatcttatat tttcatatgt atatccttgg tattccgagg gagtgttata agtaatataa
    33301 ccgtttacag aatcccttac aggagctgaa tagacctctc cgtcaggaat attggattta
    33361 ccgtcgcatt ttattgcggg aagccctttg atggagaagc ttaaatctgt tccagtccct
    33421 atgattctga cacggtcggt attattcatg agttctatca gcggatccat agccttggac
    33481 atttttgaat agtccaggtt gcaaacatcg aaatagaagt cctcaaactg ctctttgctc
    33541 atattggcca tctgcgccat tgaactgtta ggaaacctca ttatgcacca ttttgttttt
    33601 gccactcgga tattggtatg cactgtcttc gaccagaagg attgataaaa acccaacttt
    33661 tcactgggaa tatcagccca ttcagaagag ttgtaagcgc ctcttattcc tatatatgcc
    33721 tgcatatcct tcattctttc cgcttcatag tgcgcagtcc tggacatatg ctccgcatcg
    33781 gtaccaagta tcaattctct catgagagat ctgtttttta ttgtcaggaa tggtttgcct
    33841 ccaatcttgt agatttcttt tacaagctcc gaagcaagct cttcaccgga gtcaaatact
    33901 tcgataagga tattttcatt tggtttaagc tggacggaat aatttaaaag gttaattgcc
    33961 atttgtttaa tccggggatc tttcattaat agcgcctcct gttaagtgaa ttttaatatg
    34021 tatattctat gtattattta ataatataat aatattatga caggcttttt ctccattaga
    34081 cttcggttac aaaacataat acaattataa aatttgacat acataaaaca atgctgttat
    34141 tattatagct ttttggcaaa tagttgtaaa gtgggaatgc cgtacatggg gttatcataa
    34201 agtgtaaaac atataatata tgatgtttga gatgcagcat ataaggagat gaaagaatga
    34261 gcgtcataga acacgcctac ggaaaaacct tcaagacgat attatgggct gtgaaccctc
    34321 tgaaaaaggt attcaaaaag accttgtgcg aggtccatgt atttattaat gaaaaagccg
    34381 ttgaaatact caggaatgac ggatttcaag atgcatatga attcttttcc ataaacaaag
    34441 gctgcctgaa tgacggggcc gtctgggcag atcaggattt caagagcagg gagcatttct
    34501 acaacccttt tacccacaga ggtttatatg gctgcaagtc atcaaagcat ttgttcagga
    34561 aatattatgg ttgcgcattg gtatactggg actgcgggga taaggataaa gccatgttct
    34621 acctgggtgc cgccgtgcat ctggtacaag atgccacaat accgcagcac ggaagtgtaa
    34681 aactgttaaa gagccacaga aaatatgaac aatggattca caaggttcat gatgatttct
    34741 tgcattattc tgtttctgaa ggcggaatat acctggaaaa tccttttgaa tatattgaaa
    34801 aaaacgcaaa agaagccttg gaagcctaca caaaatattc acttatcaga aatccctcag
    34861 aaaaattttt caggattgca aacaggacat tccctctggc tcagaaaact acagcgggat
    34921 gtctgatgaa cttctatgcc aaaattaatg aagggtgaac tccttaagag gtacagcggc
    34981 gaaattgatt attttctttg atgagcttgg cctcagcgga aaaaacatta ctatttcatc
    35041 ctgtgaagga gtgtctgcaa atatgttgcg gagaaatacc tcataaccca cctgcttcag
    35101 tatctcacgg agggataagt ttttaaagtc cttaaaggta tccatatctg tgagtttatt
    35161 cttgatatat ccatggattt cttcctttat tgctgcatgg ttgaatctgt ttattgctgc
    35221 cggcatgctt ccgcttctga catacagcag ccaatcgagt ttcatatatt cgttcaataa
    35281 gccgaactgc tcattatcca gcctgttaac gaatgcatct tccgcaaagc acttcaaaac
    35341 agtgtataat tcattaacat ttttcggggc attgaaatgg cctttttgat tccaatagcc
    35401 tgacagcctg cggtaaaaat caaagggttc agtttccgct gcctgaagca gaaagtccag
    35461 agtatgcctg aacctgccgc tgttgtaata ctgctcaagc acattttcaa tatcctttag
    35521 ctccatgatt tctttaaagg ataaccaggc ggtgcttatt acctcatagg gaggaaaatc
    35581 atgatgctgt atgcggtatt ttttgcagcc ttgtcttatg ccggagccct tcaggagctt
    35641 taaaaaaccc agctgcagca tgtcaggatt caaagaatac acatcattga aggatttttc
    35701 gaagctttcc atatcctcat agggaagtcc ggctatcaaa tccaggtgta tatgggcatt
    35761 cttaaaggat aaaagcctgg caacattatg actcactttg tcaaaatcca tttttctctt
    35821 tatttcggac aaggtcttgg gattggtgga ttgtactccg atttcaaact ggaacatgcc
    35881 atccggcgcc tttcccgcga tctcaagaaa gcgcacatca atcaaatcag cagctatctc
    35941 aaaatggaag ttggttgatg gattaagatc aataatatac tgcattattt caatagatcg
    36001 ttccaaatca cagttgaagg ttctgtcaac aagtttaacc tgctttattc ctgcatttgc
    36061 aaaacttttg atatcctggc gcactctgtc cattgccaaa taccttaccc cccgtattgt
    36121 tgaagaaagg cagtattgac aattaaaggg acagcccctg ctggtttcat aatatataat
    36181 cctgttatca aggccgtcaa agctgtcata aggaaaagga agcatatcca aatcaggaat
    36241 gtcaggcctg ggttcagtaa tggcgacaga ttctccggag cgaaatgcaa tgcccggtat
    36301 atccggcaaa ttacttttgc cggcagtgag gaagcaaagc agatcaagca tggcagcctc
    36361 tccttcacca atgacaatat attcaatgaa ggaatatttt gaaagaagat ctgctgcatc
    36421 ataggatacc tcaggacctc caagaattat tacagcttcc ggcctggctt ttttcaagct
    36481 gctgcatata tcgagaacca tgctgatgtt ccatatatag caggaaaagc cgtatatacg
    36541 ggcattggag cagtacagct tcttcaggat gctgctgatg ctgtcgttta tgctgaattc
    36601 ggctatgtca attcctttta tatgtgcttc acaaaacttt ttcagatatc tgaccgcaag
    36661 attggagtgg atatattttg cattaattgc acagagaaga attccatttt tcataataag
    36721 cctccctttg caaatgtttc gcacatgtat taccaaatgt aaattctaaa atatatggta
    36781 taattataac agtaaagata tgatatgcat atacacctta tttaataaat tgaagttcta
    36841 gttcagactg gaggtattct tatgagagat aatgatgatt tgaaaagcag agcaaaaaaa
    36901 atttcggaaa tattgttcaa aatctatccg gaagcaacct gctccctgga ttatgataaa
    36961 cctctggaac tgctgattgc tacgatgctt gcggctcagt gcaccgatga aagagtgaat
    37021 attgtaaccc gggacttgtt taagaagtac acttcggtgt ataattatgc aagtgcaaat
    37081 cctgaggaac tggaggagga tatcaggccc acaggctttt acaggaataa agccaataat
    37141 attatcggat gctgtaaaaa gctgataaaa gacttcggag gcaaggttcc tgacaatatg
    37201 gaggatttac taagcttgtc cggagtcggg agaaagaccg ccaatgtggt attgggcaac
    37261 atatataaaa ttccgggcat aatagtcgat acccactgca aaagactgtc aaacaggatt
    37321 ggattaacga aggaggagga tcctgaaaaa atagaatttg atctgatgga gattctgccc
    37381 agggagaatt ggacagcatt tagcaacagc ctggtatatc acggaagagc aatatgcaat
    37441 gccaggaaac caaaatgcgt tcagtgccat attgctcagt attgtgactt ttttaacaat
    37501 atttaaaggg aaaatgaata aattggcgtt aaattaaata attaatagta tttgaggaat
    37561 tttgaatttt attaatagct aaatatatat taaactttta atgaaggatt ttgctgtttt
    37621 ataacgaaat agacagcata agtttcaata ctgaaatatt gtttatccag cagtgttaat
    37681 aatatcatta ttgaaagagg tggtgaagca tttgactacg gatatcatca aggggattag
    37741 ggagacagaa cttgcggctg aggagaagat caaagcagca cagcagaaag ccaaagatat
    37801 attgatgtct gcagagcaag aggctgatca tatcattaaa gccgccgtgg atcaggcaat
    37861 tcaggaaagt aaaaagcagc tggaggttgc agaaaaggag gccaatctgg aggctgaaag
    37921 caagaggaag caaaatgacg aattatgtca ggaactgagg cgaaaggctg aggaaaaaat
    37981 ggaagaggca gtgaatttgg tgatggagag gattgtgaat atgaatggca atagttaaaa
    38041 tgaaaaaagc caccatgata gctctccagt ctgaaaaaaa tgagatactc aatgacctgc
    38101 agagattcgg ttatctgcag gtagtgaacc ttgaagagcg aatggaacaa ggcttcgaag
    38161 agttgcagcc cgacagtgat aatgaagctg tgggcaggct ggaggcgaag ctttcgcaag
    38221 taaaatactc cattgatttt ttaggcaaat tcaatacggc aaaaaagcct ttgtttgctc
    38281 aaaaagttca ggtggatgaa acaaaacgca aagagtacct tgacaatgaa gaaaagctca
    38341 atgagattta tgaaagctgc agaagcattg acagcagatt tacagagatt aaaagctctg
    38401 aaacaaaact gaacaatgca atagcgcagc ttactccgtg gctggcattg aaagaaaaac
    38461 ttgaggagat aggcactact cataatacca actctattct ggggtttata gcaagcaaat
    38521 atgaagatga gcttagtact gctgtaaaag atgaggatct gactgcatat ctggaaaaag
    38581 taggcgtaga gaaggaaaat tcatatttta tggtgatata ccatcaaaca tgtgaagaga
    38641 gaatctctca actccttaag caggtcggat ggacaaaggt aacctttagc gaatattcag
    38701 gaacccctga agaaaacatc aataggatta aagcagaaat cagcaatttg gagaacataa
    38761 aaaaagagct ggcaaaaaaa gctgaagcaa tggtacctga atcagggttc cttgaaatat
    38821 tatatgattt gctttcaata gaaaaagaca gacgcagcgt agtaaaggat ttcattaaaa
    38881 cggacaagac atttatgctg cagggatgga taccggagaa atttgccgat gatatgacaa
    38941 agctgctgga tggtattacc gataagtata ccataagctt tgaagcccca aatgaagagg
    39001 atgagatacc ggtacttctg aataatccaa aaccggtaaa gccttatgaa atgataacag
    39061 acttgtacag tataccgaat gcaaagggta tagatcctaa tatttttatg gcaccattct
    39121 ttgtgatttt cttcggaata atggtaacgg atgctgtata cgggatattt atgtcagctg
    39181 ttaccggcta tgtactctac aagtataaac cgcaaggcgg aatgaaaagc atgatgtgga
    39241 taatgttctt tggaggtatt tccacattct tttggggagg aatcttcgga ggctggcttg
    39301 gagatttgat taaagtaaag ccatggtggt tcaatccttt ggatgaaccc ctcaagatgt
    39361 tgatattctg tttaatcctg ggtgtaatac atctgtatac cggatacatc ttacaggcat
    39421 accaaaatat caaagcaggc aattttatgg atgcaatata tgaccaggtg ctttggctta
    39481 tgctgctgac cggtcttatg ttcctggcac tgccgcctct tgcggaaata ggcaagtaca
    39541 tggcgatatt aggggcagcc ggtacagtga tttttgcagc aaggtctgag aaaaacattt
    39601 ttaaaaggtt cacatcagga gtattggcct tgtatggtgt aagcggattc ctgggtgatg
    39661 tgctttcata ttccagactg tttgcattat gtcttgcaac aggcgttatt gcccaggttt
    39721 ttaatgcaat gggtgttatg atgggtggca gcattattgg aaagataatt atggtcgctt
    39781 ttctggcttt tgcacatgta ttcaacacag ctcttggagt attgggagca tatgtgcata
    39841 caagccgtct ccaatatgtt gagttctttg gaaaattcta tgaaggtgaa ggaaaacctt
    39901 tcaatcctct tagaataaaa actaagtata tacaagaata ataaagaaat taattttttt
    39961 aacaaaaagg gaggcaatat aataatggaa aacttgattg aatttatggg taattctggt
    40021 ttgtatctcg catttcttgg ttcggcaata gcagcaggcc tggcaggctg cggatctgca
    40081 ataggagttg gtatagcagg tcaggcagca gcaggagtaa taactgaaga cccgagcaaa
    40141 ttcggtaaga cgcttctttt gcaggcactt ccaggaactc agggaattta cggtttgttg
    40201 attgggtttt taatttggtc aaagatcggt gtattcggaa caatactgcc tgtcaccata
    40261 gaacaggggc ttatgcttat gttggctgga atacctattg gtattgttgg acttatatct
    40321 gctatttatc aggggaaagc atcagctgca agctgtggaa taattgccaa gcggcctgaa
    40381 gaattcggaa aaggtatgat ttatcctgca atggttgaga cttatgctgt tctttcattg
    40441 ctggcatcat tcctcatgct taacggcatc aaattataac gaagcgaaaa gatgtcccca
    40501 cttctataag tggtgggtac cgctttagtt tcctaacagg ggttgagcat atctcacgcc
    40561 tcgttgaaac gctcgggtag gtttttgcag gtgtgcaaaa accctcattt aaatcttgtt
    40621 ataagggagt ggcttaaatg gcaggagtag aaaacattaa ggagaaaatc ttacaggatg
    40681 cagatttaag agcgaaagaa atattggaaa aagcgagact ccaagccaat gaaattgtag
    40741 aaaaggctaa acagaaagcc gcatccagag caaaggagat ttcgcagaaa tctgtttatg
    40801 atgtcagtga aaagaaaagg ataattaatt caatcgttga gcttgagatg cgcaaggatg
    40861 tattggctga aaagcagaag gcaataggag aggtttttga taaggttctt gagagaatga
    40921 acaacttgga agacagcagg tatgagaaag taatcataga catgttggcg gcttcggctg
    40981 aaagcggaga agaagagatt gtcttctctg aatcaggcag aaaaaagctt tcttcagatt
    41041 ttctcaataa actgaataag acccttgcag ctgctggaaa aaagggcaat ttaaaaatat
    41101 cggatgaaac caggaatatt tccggaggct tcattttgaa gagccaggga gtagagatta
    41161 ataactcttt cgaggcaatc ataaggctct atagagatga aattgaaccg aaggtagctg
    41221 agattttctt ccgggcataa taattatgca gaactcgtta actggaagaa aggaggataa
    41281 gctcaatgag tgaaaataaa tacctatatt ctgttaccag aatacgagca ctggaaacga
    41341 agcttcttga caaggcaaaa atagagagaa tgatagaagc caggaacgca gatgaaatta
    41401 ttaagatact gtatgaaaca gaatatgcaa actcaatttc tgagatgaag agtgttgaag
    41461 actatgaatc agtgctctcc aaggagcttt ctaataccta taagcttctt tcggaaatat
    41521 ctccggttcc ggaactaaca aatctgtttc agctgagata cgacattcac aatttgaaga
    41581 ctcttctgaa aagcagttac cttgatgagg aaaatgacga attattgagc gaaattggca
    41641 ctataccgat tcagcagctt aaaggaatga tcaaagagaa ggttttcagt gatttggacc
    41701 cccttgtaag agaatccttg gaagaagttg tgggagagtt cacggttaac cccgatccgc
    41761 agctaattga tgtgactctg gacaaggctc tgtataaact gatgtataaa gcggcaaagg
    41821 ataacaagag cagctttttg acggattata tatcgacaca gatagacctg ataaatatca
    41881 agtcgctgat tagagtaaag tcaatgggct atgggcggga attccttaaa aaagtaatac
    41941 ttgaaaacgg aaagctggat tatgcattct tcagcgatat ctttgatgaa tccttggaaa
    42001 cactgattga cagattggcc tacaaggatt acggaaaggt agtggaagaa ggtataagca
    42061 actatattaa gacgaaatcc ctgactaagt ttgaaaagct ttctgacgat tttatatttg
    42121 aactcgcaaa gaaaggcaag tttgttgcat ttggaatcga acccctgatt ggttatctga
    42181 tggccaagga gaatgagaca aagattatca gaatgataat ggtcggaaaa atcaatgaga
    42241 ttccgaatga attgataaga gaaaggctac gtgatgtata tgtataagat tggagtaatt
    42301 ggggacaagg actcgatact gggttttaag gctttaggga tatcggtatt tccgaccaac
    42361 gatataagag aagccaacag attgattcac aagctggcaa gggagaatta cgcagtgatt
    42421 tttgtaacag aacagcttgc caaggatctg gacgaagcaa tcggtgaata taagaattcc
    42481 ttgactccgg caataatcat gattcctaat aataggggaa ctttgggatt agggatagac
    42541 ggcgtcaaaa aatcagtcga gaaagctatc ggttcagata tattattcgg gaaagaaggt
    42601 tgataacttg agcgatggta gaattataaa agtatcaggt cctcttgtca tagcggaagg
    42661 catgctggat gcaaagatgt atgacgttgt gcgcgttagt gataaaaatc tcataggtga
    42721 aataatagaa attagaggcg ataaagcttc aatacaggta tatgaggaaa cctcaggctt
    42781 agggccggga gaaccggttg ttaccaccgg tgagcctctt agcgttgaac tgggaccggg
    42841 gcttatcgag tcgatttttg acggtatcca aaggcctctt gacgtagtaa gaagtgcatc
    42901 cggcgaccat atagcaagag gtgtacaggt agaaaagctt aacaagacca gaaagtggaa
    42961 ttttgaacct tcagttaagc aaggagacgc tgtaaaaaca ggagatatta taggcatagt
    43021 taaagagact tccatagttg atcataaaat aatggttcct catggaattg aaggaaccat
    43081 agaggaaatc tattccggtg agttcaccat tttggaaacg gtggcaagaa ttaagacaaa
    43141 agacgggata aaggaagtac agatgctcca gaagtggccg gtaagaaagg gaaggcctta
    43201 taaagaaaaa ctttctccgt caacacctat gacaacaggc cagagaatca tagatacatt
    43261 cttccctgtt gcaaagggcg gcacagcctg cattcctggc cctttcggaa gcggtaagac
    43321 agttgtacag catcagctgg caaagtgggc ggatgcagag atagttgttt atataggctg
    43381 cggtgagcgt ggaaacgaga tgaccgacgt tcttatggag ttcccggagc ttaaggatcc
    43441 aaaaaccgga gagccgttga tgaagcgtac ggttctgatt gcaaacacat ccgacatgcc
    43501 ggttgcagcc cgtgaagcaa gtatatatac cggcataaca ataggcgaat acttcagaga
    43561 tatgggttat tccgtagctc tgatggcaga ctcaacctcc cgttgggcgg aagcacttcg
    43621 tgaaatgtcc ggacgtctgg aggaaatgcc cggtgaagaa ggttatcccg cataccttgg
    43681 ttctagagcg gcagagttct atgagagagc cggaaaggtt gtatgcgttg gaactgaaga
    43741 cagagaaggt gcccttactg cagtcggtgc ggtatcacct cccggaggag acttgtcaga
    43801 gcctgtaacc caggcaaccc taagaatagt aaaggtattc tggggcctgg atgctgctct
    43861 ggcttacaga aggcacttcc cggcaatcaa ctggcttcag agttattcct tataccttga
    43921 cagattgggt gattggttta cagaggaagt agcgcttgac tggaacaacc tcagaatgga
    43981 aaccatgaga ttgcttcagg aagaggcgga actgaacgaa atagtgaggc tggtaggttt
    44041 tgatgcccta tcattaaaag atagatttac attggaagca tccaagagca taagggaaga
    44101 ctatctgcat caaaatgcct tccacgaaat tgacacatat acatcaatcc ataagcagta
    44161 cagaatgctg aaagcaatac tgctgttcta tcacgaagga caaaaagcat tgagcgaagg
    44221 cgcattctat aaagagctta ctgctcttcc ggtcagggag gatattgcaa agtctaagct
    44281 catacttgag aatgatatcg aatcctttga tgttcttgaa gctaacatta gagatcagat
    44341 agcttccatc ctttcgaagg gagggaaaat agtatgctaa aggaatataa aaccattaaa
    44401 gaagtagcag gaccgcttat gtttgttgac caggtagagg gcgtaaagta cgacgaactg
    44461 gttgaaatcg aattaaaaaa tggggagatg cgccgcggaa aagtactgga ggtttctgga
    44521 agttcagctc ttgtgcagct gtttgaaggc tccacaggaa taaacatcaa tgagggtaag
    44581 gttcgtttta cgggacacag ccttgagctg ccggtgtcta cagatatgct gggaagagta
    44641 tttgacggct tgggtaggcc gaaggacgga gggcccaaaa tcatacccga atcaaggaaa
    44701 gacataaacg gtaacccgat taatccggct gccagagact atccctcgga gttcatacaa
    44761 accggaatct ctgctattga cgggcttaat acactggtaa gagggcagaa gcttcctgtt
    44821 ttctcaggct cgggccttcc tcacgcaaga cttgcagccc agattgcaag acaggcaaag
    44881 gttttgggta cagaaagcaa attcgccgtt gtatttgctg ctataggtat aacctttgaa
    44941 gatgcggatt actttgtatc cgacttcaag agaaccggtg caatagacag ggcagtgctt
    45001 ttcatgaatc ttgcaaacga cccggccatc gagcgtatag caacaccgcg tatggccctg
    45061 acagcagcag agtacctggc atatgagaag gatatgcacg tgcttgtaat aatgacggat
    45121 ataacaaatt actgcgatgc tcttcgtgaa gtatctgccg cacgtaagga agttcccggg
    45181 agaagaggtt atcccggata cctgtatacc gaccttgcta ccatatatga aagagcaggg
    45241 aggattaggg acaggaaggg atcaataact cagataccga tactttcaat gcctgaagat
    45301 gacaagaccc atccgatacc tgaccttacg ggatatatca cagaaggcca gataatactc
    45361 agcagagagc tttacagaaa gggtgtaatg cctcctatag acgtacttcc ttcactttca
    45421 cgtcttaagg ataaaggtat aggcagagga aagacaagag aagaccatgc cgatacaatg
    45481 aaccagcttt ttgcatccta tgcaagaggt aaggaagcta aagagcttgc cgtaattctt
    45541 ggtgaaggcg cccttagtga tacagataag ctttttgcca agtttgctga tgaatttgag
    45601 aataaatatg tgtctcaggg agatagtgag gacagaagca ttgaacagac acttacactg
    45661 ggctgggagc ttctctccat actgccgaaa gctgaactta agaggatcaa ggacgagtat
    45721 attgataaat acctgcctaa aggggatgca tagcgtatgg ctaaactaaa tgttaatcct
    45781 actcgtatgg agctgaccgg tcttaaaggc aggctgaaaa ctgccaaaag aggccataag
    45841 ctgcttaaag acaagcagga tgaacttatg aagaagttca ttgattatgt taaaagaaac
    45901 atggagctga ggctggatgt ggaaaaggag cttaccgctg cttttaagaa ttttcttatt
    45961 gcacgagcgg tgatgtcttc agaagtgctt gaagaagcaa taatgtaccc cactcagaaa
    46021 attaaccttg agatgagcac aaaaaacata atgagtgtaa attcacctgt attcaatctt
    46081 gtgaaggaag aaaacaaaga ggcttcaagc atatatccct acggctttgc agctacctcg
    46141 ggagaactgg atggggcaat attctccctg caggaggttt ttgacaagct gattgagctg
    46201 gctcaggtgg aaaagacctg tcagcttatg gccgatgaga tagaaaagac cagacgcaga
    46261 gttaacgccc tggaatatgt catgattccg cagctcattg aaacagtaaa gtatattacc
    46321 atgaagctag acgaaaatga gagaggaagc agagtaaggc tcatgaaggt caaggacatg
    46381 atggaaaagc agaaagccga ggcataaagc aatccgcagg aacattcctg cggatttttt
    46441 ctattgtttt gcttctacaa aggtagtgca gcaggttcct tcactggaat gggcaacacc
    46501 gtcacccatc ggattcactt caatcacact ggcgctgcag tagttattgt cataatatga
    46561 gcaggtgtct acattgcatt ttatgctgtg attgtgggca tgcattttat tggatgaaat
    46621 gtcgttgctg cttatatttt tatgcataaa aaagcctcct tgtaagttta tttaatatat
    46681 atttttcgca gtattagaat ataaataccg aattaaatgt gttaaatagt ggcaggttca
    46741 atccggaaga gtgaaaagtc agactttaaa cacatgcact taaaaaattt tttgacatgt
    46801 tcaaaaaatt ttattaataa aatgccaaat gatatggaaa tatgtattaa tttaatgtaa
    46861 tatataggtg gaggatggat acaagatggc ggtaaaatta tgggggtgtt ttcaaaaaaa
    46921 tgggaattca gtttgctaac aggatggaca acttaaaagg ttccgtaatc agagagttgc
    46981 tgaagtatgc cgaacagccg gacataatat catttgcagg agggcttcca gcgcctgagc
    47041 ttttcccggt ggaagaaatg atgaaagcaa cagatagagt aatgatggaa gatggaagag
    47101 cttcattgca atatggtgca actgacggat atttaccgct aagaaagaaa atagctgaaa
    47161 ggtctaaaaa gattgggttt gttccggatc ccagtgaaat actcataact tccggatcac
    47221 agcaggggct tgatttcacg gcaaaaatat ttataaacaa agatgatgta attataaccg
    47281 agagcccaag ctacctaggt gctttaaatg ctttcaaggc atatcagcca aagtttgtag
    47341 agattcctat ggatgaagac gggatgataa ttgaagagct ggaaaaagct ctggcagcca
    47401 ataaaaatgt aaagtttatt tataccatac ccgatttcca gaacccgaca ggaaggacaa
    47461 tgcctgttga aagaagaaag aaacttatgg aattggctac tgagtatgaa atacctgtaa
    47521 tagaagataa tccatacgga gaattgcggt ttgaaggcga aatactgccg tccttaaagt
    47581 gctttgatcc caagaacctt gttattcttc tgggaacctt ctcaaagatt ctggcaccgg
    47641 gcatgaggct tggatgggtc atagcccgcc ctgatttgtt gataaaatac aatttggtca
    47701 agcagggagc cgacttgcaa agcagctcta tggctcagag acaggcaaat atgtttatgg
    47761 aaatgtacga tattgaaaag catatcgaaa aaatcatcaa agtatattct gcaagaagga
    47821 accttatgct tgatacaata aaaaaggaat ttccgtcaaa cgtaaagtgc actaatcccc
    47881 aaggcggact ttttgcatgg gctaccttcc ctgaagaggt tgatgcttcc gtgattctca
    47941 agaaagcttt ggaacagaag gttgcgtttg ttccgggcga gcctttctat cccaacggcg
    48001 ggaatgcaaa tcactgcagg cttaactatt catgcatgag tgaagataag atagtagaag
    48061 gaattgcaag acttggcaaa actttaaaat caatgtaaac agcattaaaa ggctccggga
    48121 tatcccggag ctttttaatg taattggcat tcacacagca aaggcaggaa tcggccgtta
    48181 atatatttga attgtaacaa atttggtaat aataaatgta tatatggcaa aaacacaaat
    48241 ataatagtat aatatgcata accttattag ttgcatacat aaacatatta gaaaaggagg
    48301 aaaattgatg gaaaaatgga cttgtacagc atgcggttat gtttatgacc cggaagcagg
    48361 agatccggac aacggaattg aaccaggcac tccttttgaa gatattcccg aggactgggt
    48421 atgtccgttg tgcggagttg gaaaggacat gttcgaaaag gaataaaaga agaaatatta
    48481 tttagtttgc acgagaaaaa ctgagcaagc ctcagttttt ctattgtaaa caattattat
    48541 aaaaaaattg tgtattgcta aaaatagtga tacatgataa aataaatttt gtcggtaatt
    48601 ccgaactaat attcccggtt tgtgtaatgg tagcacaact gactctggat cagtttgttg
    48661 aggttcaaat ccttaaccgg gagcca
//
LOCUS       DAOQUR010000017        45808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_908_length_45808_cov_12.033703, whole genome shotgun sequence.
ACCESSION   DAOQUR010000017 DAOQUR010000000
VERSION     DAOQUR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 45808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45808
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_908_length_45808_cov_12.033703"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..2293)
                     /locus_tag="PKI50_05470"
     CDS             complement(74..2293)
                     /locus_tag="PKI50_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT03194.1"
                     /translation="MKVKIKLLLLALVYMTTTLIPLTVHAQVHQSPQIQWQKAFGGSD
                     NTEISEVIQTSDGGYLAIGTTNSPDGDITTKQRVNEPGSGFREDDDLLVVKFDSNGQP
                     VWHQTLGGSAGDMGFAVRQHSDGTFILAGMTFSKNGDVVSNQNNPSSDFVVWMVKLSA
                     KGDILWEKVLQDVDTEASKVISLHIIDDGYLMGIQGGKNNFYNAYAVRLDFQGSIRWQ
                     KTLPGYYLYDIAQLNNGDFISAGDFIVRFTQDGDILWQEPSVKEEQSIEGTADGGFVW
                     AVSVNQYHRELIDLYKSVTKRSFPMALYSDTYNTSPIIMVVKFDSGKNPQWANYLGGS
                     RNEHVISIKQTSDGYYAILARSESRDGDISDSKGNSDFWLTGLSSEGILIWEKSLGGS
                     NADEPKSLVAEKDGYIVAGYTNSNDKDVKGLHNPTKSYSYDAWIAKLGWNEKLDTYAG
                     NYPSSWAQTDVEEAKTLGLTSNNLLGSYTENITREDFSELVVRLYEILTKHEAPLPEA
                     NPFNDTVNLYVLKACELGIVTGAGNNMFMPKDSLTREQASLMFFRLMNKIKPGLDTHV
                     SSPKMFEDHTNISNWAVDAVYYINYRGIIQGSNNQMTPKGALTREQAIILAKRMYENI
                     DKYQLDEFRLVQQSPIIVSAGQTININVIVRGSDFSNSEKITTWKVDDNSIASISGDI
                     VGGYTNWILGEVAEKTVIISGYSASVTGNKEGTTRLTVFIGERSQVQSRTYEIIVKR"
     gene            complement(2330..3850)
                     /locus_tag="PKI50_05475"
     CDS             complement(2330..3850)
                     /locus_tag="PKI50_05475"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HNT03195.1"
                     /translation="MKCINLFIRYKHWGKIVFTLAMSMIIILGGLLEVYAITFSGSLP
                     IGSYVQFGMYKDKPIVWMVVHNDENGSILFAKDIITVKAFDASGDRAEGRDSKYNTRV
                     AEGSNYWTKANLCEWLNSEEQTVNYSHQNPDAANTMGVSYSTFVNGKSSILAEDVSYA
                     DEPGFLTNFHDMEMAAIQTISLRTYLSEFDLPVKDGGNQPWNLNGDNCNAYYKLSSHK
                     IFLLSVDEVKAWIEENELNRTAPWYTAPNGWQINTYWLRTPDYDYAASVMTIPSNKLS
                     DPHGFNAYQANGVRPALCLKPGYSMSGPGTEYQPYVIRYEVPEEDAEGDISDKPSFWA
                     EEEVTRAIRNNLVDESLLSQYQNDITRQEFCTLAVKLYEELSGRKATYDMVTPFSDTN
                     DISVLKSYNLKIIQGVGNGRFAPNENISREQIAVMFYRILEAILPSRPDIQLKTSHEI
                     RFYDNNEISDWAVKGVTVMNGEKVILGTGTGFNPKGTATREQAFILANRMFEKYHR"
     gene            4083..5558
                     /locus_tag="PKI50_05480"
     CDS             4083..5558
                     /locus_tag="PKI50_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03196.1"
                     /translation="MEFHDKLNFLMDITKTTNISLARASSMDPSYISRLRNGKRKPVK
                     NASYMIVFARFFSKRIHSEKQMDVFREVLLPYTMFPESESALTDFLHHWLVNNDFKNG
                     HLNLPVLHRLTDAEPRGGFNKNLNENVKPFFKTACGLYQGIQERRLAAVHFLSTILNQ
                     NSPRTLFFFSDEDMSWMTGDPVFYQKWSHLMGQIIARGNRIKIIHDLTRGLDEILSSI
                     QLWIPLYLSGAIEPYYYPRKRDGIYKRSLYIAPGLCAVSSDSIGTMTADESTAVFHQD
                     LRMVEALENEFHHYLNLCRPLMSIFTPDKHQNYLSLLESFEREYAPTIFKTPHLSMLS
                     MSESLMESILLRYCKTSYNKNRSFYNKRLENFRKTLKRHSFTEIFSIPEKEALLAGKA
                     RINLPNPFESIEGFYTPDDYRRHLDHVAVLTKHYDNYNVCIEKNECSLGYTLYAKEGI
                     GVIVAKTKTPAVVLFIQESNLFAGFWDLLIEMKEHGARHDV"
     gene            complement(5819..6790)
                     /locus_tag="PKI50_05485"
     CDS             complement(5819..6790)
                     /locus_tag="PKI50_05485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT03197.1"
                     /translation="MFNKKCRLLLVSLALVLLTVFPTACNMVRVNPEVDNKTVVAKVS
                     GKEILKEEFNKMFDIFKTQYEQQFGTEVWDQEVEGRKFGDVAREKLLEMLIDEKLQME
                     KAAELGITVTDDEVNAEIENARKYFDSEEKLNEFLKGQGIDLEYFKESVKKELTINKL
                     VEKLTEDITVTDEELKNYYDTHQDEFMNVKASHILLDTKEEAEKMLTRVKAGENFAEL
                     AKQYSKDPSAKENSGDLDYFRHGDMVEPFEKAAFALKPGEISEIVQTDFGFHIIKVED
                     SKRDKFEDVKEMLKGKLLYEKKGSEYEKLLEEMRKNANIEKFVKNLK"
     gene            complement(6841..10359)
                     /gene="mfd"
                     /locus_tag="PKI50_05490"
     CDS             complement(6841..10359)
                     /gene="mfd"
                     /locus_tag="PKI50_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNT03198.1"
                     /translation="MSIGNVLKPLYEMEEFNELLSSLEANLNTVEVYGISDTQKAAAA
                     AFVCRHFGKACLFITYNDLLSRKIYEDISFFEPDMAALMPGRELVFHRIDARSNEILQ
                     NRLRAYDDIISGKNCIVCTSIEALLTRTVSPELFASMTRELSVGQTVSLDEIKEYFIK
                     AGYERIEMVEGKGQFSIRGGIIDFFSPIHQNAVRIELFDDEIDSIRYFDVLTQRSISK
                     TKKVKITPAREFIVKEEEFLEGAEKLQSELSARLRASGNERRRKSSDIRVREKISEDI
                     EKMRQGIHFEGIEKYSPYFSDKNYSLIDYMGDCVIFIDEPNRIKQRCEAIGTEFMEHF
                     NQLLEKGEVLPGQFEALFTYEDVVPKIREKKTVCLDSLYKSSSDFIPQKTISITSRSM
                     HPFHGKLSMLMEEIAVWRKNRYRVLILSGSRDRGLRLASDLRDRDIDAVYKDELDIEL
                     KEGHVIILPGILNSGFDFPSIKYAVISDREPFGSKSRKKIEKKKGKAINVFTDLKAGD
                     FVVHENHGIGQYIGIEKLKIDNTTRDYLHIRYSGNDKLYIPTDQLDMIQKYIGAEDKG
                     PKLNKLGGTEWVKVKAKAKKAIEAMAMDLLKLYAERQQAVGFEFSEDTRWQKEFEDMF
                     QYQETQDQLRCIEEIKKNMEDSRVMDRLLCGDVGYGKTEVALRAIFKCVMDNKQAAIL
                     VPTTILAQQHYNTCIQRFANFPVNIDVLSRFKTHAEQSRIIQNIKNGNVDIIIGTHRL
                     LQNDVEFRDLGLLIVDEEQRFGVAHKEKIKSLKKNVDVLTLTATPIPRTLHMSLSGIR
                     DISIIEEPPEERYPVQTYVMEYNEEVIKDAIAKEIARGGQVYYLHNRVRSIQKIAAKL
                     KAMLPDARVAVAHGQMEEKMLEDTMLDFYNNEYDVLVCTTIIEAGLDIPNVNTIIISD
                     ADKMGLSQLYQLRGRVGRSNRLAYAYLTYQKDKVLNEVAEKRLQAIREFTEFGSGFKI
                     AMRDLEIRGAGSLLGREQHGHLEAIGYDLYIKLLEDTVKELKGEEVTETVDTSIELKI
                     SAFIPESYISDENQKIEIYKKIAYIGSQEDIYDIEEEIEDRFGDLPQVVRNLLDISYI
                     KHLAGKCNVVSITQKKNSILVKFSSDRSIKPETAIRVAGEYIDRLLFTASGQPYFTIK
                     VDEDRPDEYIKFLKEFLGKISSFQNG"
     gene            complement(10425..10991)
                     /gene="pth"
                     /locus_tag="PKI50_05495"
     CDS             complement(10425..10991)
                     /gene="pth"
                     /locus_tag="PKI50_05495"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNT03199.1"
                     /translation="MYLIAGLGNPGDKYKYTRHNVGFMVLDYFASVHNISISRIKHKA
                     LLGEGMIGEEKLVLAKPQTFMNLSGESIYELVNWYKCDISKLIVVYDDVDIDIGRIRI
                     RTEGSSGTHNGMKSIIYLLQTDQFPRIRVGIGKQPDFMDLADYVLSRFSDEEIPLMEE
                     AVKNSSLAIEEIIKNGINSAMNWYNREK"
     gene            complement(11025..11972)
                     /locus_tag="PKI50_05500"
     CDS             complement(11025..11972)
                     /locus_tag="PKI50_05500"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNT03200.1"
                     /translation="MNVHGKSIKVLNCNANKDLAKDIAFNLGIPVGDAQVSTFSNGEI
                     GVSINESIRGADVFVVQSTCTPVNDNLMELLIMIDALKRASAGRINAVMPFYGYARQD
                     RKSKSREPITAKLVANLIEAAGADRVITMDLHAPQIQGFFDIPVDHLYAAPLIVNYFI
                     NKKLDDIVVVSPDVGGVTRARSLATKLDTPLAIIDKRRPKPNVSEVMNIIGDIENKNC
                     IIIDDMVDTAGTMVNAAKVMKEMGARDIYVACTHPVLSGPAFERIRDSVIKELIVTNT
                     IPLLAGKPVDKIKVLSVAPLFAEAIKRIYEGLSLSKLFD"
     gene            complement(11997..13367)
                     /gene="glmU"
                     /locus_tag="PKI50_05505"
     CDS             complement(11997..13367)
                     /gene="glmU"
                     /locus_tag="PKI50_05505"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNT03201.1"
                     /translation="MSNVYSITPIILAAGEGKRMYSKVPKVLHKVCGLPMVEHVINCA
                     REVSGAEPVVVIGHGAEQVRKEVTGVKFVMQERQLGTGHAVMQAEKYINDGDILILYG
                     DTPLLRPDTLLEMREMHRAEGCSATILTSDMDDPAGYGRIIRIGEKLIEAIVEDKDAD
                     SSTKLIKEINSGIYFFDGKELQEALGYLKNDNTQGEYYITDVIGIMRKKGLKIGAFKI
                     KNAEEILGVNNRYQLSEANKIMQRRIVKKLMLEGVTIIDPDSTYIDASVTVEKDVTIY
                     PGCIIEGNTVIQEDSVIGPYSRIKDAKIDRGVKIQNSVVLESSIGEGTSIGPFAYLRP
                     GNKIGKHAKVGDFVEMKNSTFGDNSKASHLTYVGDGDVGSNVNLGCGVVFVNYDGKNK
                     YRTYVGDNSFVGCNVNLVAPVRVNENTYVAAGTTVTKDVPQDSLAIGRARQENKEGWV
                     TKRKNK"
     gene            complement(13508..14347)
                     /gene="nadC"
                     /locus_tag="PKI50_05510"
     CDS             complement(13508..14347)
                     /gene="nadC"
                     /locus_tag="PKI50_05510"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNT03202.1"
                     /translation="MDNIIIDRIIKNALREDLGWGDVTTDSTIPDTALIRGNFIAKEE
                     GIICGIEVCRRVFEMVDGTIDFQARMKDGQRAFKGDIIAEISGNARSILKGERTALNF
                     FQRMSGIATMTSKYVSEISGLKTRIADTRKTAPGLRILDKYSVKAGGGFNHRFNLSDM
                     VLIKDNHIKAAGGITPAVAAARAKGSHGLKIEVEVESIRELIEAIEAGADIVMLDNMT
                     LDMMKEAVSIASGKVLLEASGNITIGGERSVRAVAETGVDIISAGALTNSVKAMDISL
                     RFL"
     gene            complement(14362..15918)
                     /gene="nadB"
                     /locus_tag="PKI50_05515"
     CDS             complement(14362..15918)
                     /gene="nadB"
                     /locus_tag="PKI50_05515"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNT03203.1"
                     /translation="MPLRYLTSFDTRKIESEKWDVVVIGSGVAGLYSAVNLNPGYKVC
                     VLSKETMDENNSYLAQGGIAAAIGEDDMPEYHFMDTIKAGAGHCNEEAVTVLVEEAPK
                     DIKLLEKLGTNFDREPDGTLTRTREGGHGRFRIVHALGDATGKEVVDSLLRVCRDREN
                     ITIKENCFAIDLLVSEGRCIGVLVATGIDTEATKKVLYCRSVICASGGVGQVYRNTTN
                     SEVVTGDGISMAYRAGAVLSDMEFVQFHPTAFYTPEYTGNRFLISEAVRGEGGILLNK
                     NHERFMYKYNEMGEIAPRDIVSRAIFSEMKKTGSEYVYLDITHKDSDYLKKRFPTIYK
                     SCLEHGVDITKDYIPVSPVQHYFMGGIQTDLLGRTNIKCLHACGEAANTGVHGANRLA
                     SNSLLEGLVFGRRSAEYINAGIKDIKLEEVHVSNVYGASGKTLNVMDIRNEIKTLMDA
                     HAGIERNGPDMEKSLNRINEIIGSLEDAEMGSVSAMETLNIAYIASLILKGAIARKKN
                     CGSHYRTDSE"
     gene            complement(15931..16839)
                     /gene="nadA"
                     /locus_tag="PKI50_05520"
     CDS             complement(15931..16839)
                     /gene="nadA"
                     /locus_tag="PKI50_05520"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNT03204.1"
                     /translation="MQNNLAEEIQRLKKERNAIILAHNYQVPEVQDVADVVGDSFSLS
                     QHAAETDSDVIVFCGVHFMAESAKILSPDKTVLLPVRDAGCPMADMVTAPKLREMKAR
                     YPDAAVVCYVNSSAEVKAESDVCCTSSNALKVVESLENKQVIFVPDKNLGSFVASKVK
                     NKEIILWKGFCITHKRVKVEEVQKIRQLHPNAKILMHPECEPEVQKLADFLGSTSAII
                     KYAGEIPEKDVIIGTEEGILHLLKRQNPDKNFYLLSTGLICTNMKKTRLDDVHSALLN
                     MQHEIHVDEDIRIKALKSLERMLKIK"
     gene            complement(17039..17374)
                     /locus_tag="PKI50_05525"
     CDS             complement(17039..17374)
                     /locus_tag="PKI50_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03205.1"
                     /translation="MTDKKKQYPKAEKPEMDMHDMEIPEMEMPMHGCPMICPMMCPMM
                     HHGEWQGMEDMRMPDWYEEDDSDYSSDESDEYPVYWKHKKKHKYSPYPFYWPPFYPVK
                     PYKKKHKRW"
     gene            complement(17495..17755)
                     /gene="spoVG"
                     /locus_tag="PKI50_05530"
     CDS             complement(17495..17755)
                     /gene="spoVG"
                     /locus_tag="PKI50_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HNT03206.1"
                     /translation="MEITDVRIRKVNAEGKMKAVVSVTFDNEFVVHDIKVIEGQEGLF
                     IAMPSRKTPDGEFKDIAHPINSSTRERLQTSILEEYGKVKNE"
     gene            18215..19594
                     /gene="murC"
                     /locus_tag="PKI50_05535"
     CDS             18215..19594
                     /gene="murC"
                     /locus_tag="PKI50_05535"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNT03207.1"
                     /translation="MFSLNNVKNVHFVGIGGISMSGLAQILLESGYNVTGSDINDSNL
                     INKLRKNGAEVTIPHNPKNVETAELVVYTAAVKQDNCEIIRAKELGIPVIDRATFLGM
                     IMKNYNYGIAVAGCHGKTTTTSMISIIIKNAGLDPTALIGGEIEAIDGNVRVGKSEYF
                     ITEACEYCESFLKFYPFVAVILNIERDHLDYYKDLEHIISAFRKFAKLVPADGALVVC
                     SDNENTLNVSKDVECKVLTYGIKNKADFYARKIKYDKLGHPSFEVMHRDKKLGTFSLN
                     IPGEHNVLNALAAIAAAYNAGVSMNIVKKSLKEFKGTYRRFDIKGVRNNITIVDDYAH
                     HPTEIKATLRAAKQFPHQKIWCVFQPHTYTRTRTLFKEFSEAFYDADNVIITDIYAAR
                     EKDTGLVNPSELVDSINKCSANAVYRNDFRCIANLIAHEAKPGDLIFTMGAGDVFKLG
                     LMILDEIKI"
     gene            19739..21331
                     /locus_tag="PKI50_05540"
     CDS             19739..21331
                     /locus_tag="PKI50_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HNT03208.1"
                     /translation="MEESSINSTYHTINHWNNFKLKLVFEGVIVGAFSGLVVVLYRYL
                     IELAGHFSKKIYLMQKGNNLFIVLWLVFLIFLGYFIGVLIKKEPMISGSGIPQVEGVL
                     LRYFSMNWWKILIGKIAGSILSIGAGLSLGNEGPSIQIGAAVAKGYCETFKKKKLEEK
                     FLITSGASAGLAAAFNAPLSGVMFSLEEIHKNFSPVVLTSAMAASITADVISKYFFGM
                     KPIFNFQGISPLPLQSYHYLIILGIILGLLGAVFNYSMLKAKDLYSMQKWLPEHLRPV
                     FPFIIAGVLGLLLPEVLGGGHELIASMVNLSYSLNILMLILVVKFMFTMISSGSGAPG
                     GVLVPLLVIGALIGSIYGNVLSDVFSFNREYVKNLIILAMAGYFTAIVRSPITGIILI
                     IEMTGSFNHLLSVSIVSAIAYVTADILGSKPIYESLLDRMVNKGSSRFKGDSKTKVII
                     ETAVCMGSLIEGKKIKELKLPSECLLVAVKRGEHEIIPKGDTQIHAGDYLITLTNEDN
                     AAEIREKLTAIAEVLSASIYEK"
     gene            complement(21339..21941)
                     /locus_tag="PKI50_05545"
     CDS             complement(21339..21941)
                     /locus_tag="PKI50_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNT03209.1"
                     /translation="MHVFEYGQKEIEYLKKKDKKLGAAIDRIGMIKREVTPDPFTALV
                     SSIVSQQISSKAADTVWNRLEALVGTITPESIAQAEILEIQACGMSERKAGYIKGAAE
                     AALSGQVDFTALHTLSDEEIIKKLSSLHGVGVWTAEMLLIFSLCRPDVVSFKDLAICR
                     GMMNLYGIKELSKEKFESYRKRYSPYGSVASLYLWALSAE"
     gene            complement(21941..22405)
                     /locus_tag="PKI50_05550"
     CDS             complement(21941..22405)
                     /locus_tag="PKI50_05550"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNT03210.1"
                     /translation="MVYTYFYDYLIGRIGITEKDSKITNVYITDKELSQDMQLCETPL
                     LKEAARQLKSYFAGELKEFSLPLEPSGTDFMKQVWTALCGIPYGKTATYGEIAGKVGR
                     PKAARAVGLANNRNPIPIFIPCHRVIGADGSLTGYAGGLDMKKKLLELEKDK"
     gene            complement(22402..22971)
                     /locus_tag="PKI50_05555"
     CDS             complement(22402..22971)
                     /locus_tag="PKI50_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HNT03211.1"
                     /translation="MKEHFRISDNEKWQAVVNCDKSYDGLFLYGVKTTGIFCRPSCRA
                     KTPVRGNVIFFDNAVNAIDEGFRPCKKCRPDEAVFEPNLELVNKAKDIIEANYDKPID
                     ISHISKQLGVSTNHLTRLFRQNSGLTPKHYTTKLRISKSAELLGQADKNIIEIAYMTG
                     FNSLSNFYKHFKEQVGQTPNEYRKSRGVL"
     gene            complement(23043..23489)
                     /locus_tag="PKI50_05560"
     CDS             complement(23043..23489)
                     /locus_tag="PKI50_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT03212.1"
                     /translation="MIEEIRKLFLEYARSLEIDLDFQDFETELNTLPGKYQPPDGALI
                     LALVEGREAGCIALRKISKDICEMKRLYVRDEYRGLGLGKKLVGMIIDEAMNNNYQYM
                     RLDTLPTMKSAQSLYLSFGFYDIEPYVYNPIQGTRFMELKLKKIDW"
     gene            complement(23597..23830)
                     /locus_tag="PKI50_05565"
     CDS             complement(23597..23830)
                     /locus_tag="PKI50_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HNT03213.1"
                     /translation="MKNRDRIKVSKEKRDDMLSAIKYYFLKERDEELGDLAASLILDF
                     IIEELAPEFYNKGVCDSQKYMNDRVEDLLSIQI"
     gene            complement(23964..25139)
                     /gene="gguB"
                     /locus_tag="PKI50_05570"
     CDS             complement(23964..25139)
                     /gene="gguB"
                     /locus_tag="PKI50_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT03214.1"
                     /translation="MGIVNEARDLIKKNIREYGMYIALVVIMLTFSMATDGLFMSSRN
                     ISNLINQTGYIAVLAVGMTLIIVIRHIDLSVGYIAGFLGAIAAILLTKYAMPVWITIA
                     VVLVLGASIGIIIGFLVTKMSIPSFVVTLAGMQVFRGALLRVTSSTGTIIVPNADFNA
                     IGNGYIPKLFSINGVHGLTLVLGAAAIVLYILAEFNNRNNKLKYGFEVLSNVMFTVKL
                     LFVSLVIGYITWILAGYNGFSWTVVVVIIVVLVYNFITEKTVLGRHIYAIGGNPDAAK
                     LSGINVNRVSMIVFSSMSMLAALSGILYTARLQSATTTAGLAFELDAIAAAYVGGASA
                     AGGAGKVTGSIIGAFVMASLTNGMNLMGVDISYQYIIRGAVLVAAVIFDVKTRNKKA"
     gene            complement(25151..26674)
                     /gene="gguA"
                     /locus_tag="PKI50_05575"
     CDS             complement(25151..26674)
                     /gene="gguA"
                     /locus_tag="PKI50_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNT03215.1"
                     /translation="MKEYILKMNNITKVFPGVKALDNVNFQVENGEIHCLVGENGAGK
                     STLMKVLSGVYPFGEYSGDIILDGSVRKFAGISDSEKAGIAIIYQELALVPEMTVYEN
                     IYLGHEIKKGRLIDWNETILQAGEALKKVRLNNLNPATKVKDLGVGKRQLVEIAKALS
                     KNVKLLILDEPTAALNEDDSENLLELLRELKEHGVTSIMISHKIKEVIEIADNVTVLR
                     DGRTVASMSARDGGILEGNIIKHMVGREIENIYPKRNVQKYEEKTLEVNNWNACDSKT
                     GREILKDMSFFVKKGEIVGIAGLMGAGRTELAHSIFGNPDEYKISGDLYVMGQKKHFR
                     GPHEAIKAGVAYVTEDRKGNGLILISDIKTNITIANLKALAKQGIVNENEEVKTANYY
                     KGSLDIKAPSIEQKLENLSGGNQQKVSLGKWLYVKPKVLILDEPTRGIDVGAKYEIYT
                     LMNRLVEDGMSIIMISSELPEIMGMSDRIYVVSDGRITGELPAEGATQEKIMEMATN"
     gene            complement(26791..27909)
                     /gene="chvE"
                     /locus_tag="PKI50_05580"
     CDS             complement(26791..27909)
                     /gene="chvE"
                     /locus_tag="PKI50_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNT03216.1"
                     /translation="MKRLIAVLMAFAMLFALTACGSAPASGETGGDSKTVDVGIVLPT
                     KDEPRWIQDETRFSEALGSTDYSVEILFSQGSSAKEKENVDALIAKGINVLIICPHDG
                     DAAASAVEAARKEGIKVISYDRLITGTDAVDYYVTFDSIAVGEAQAKYLVDQASGSGN
                     PLYLYAGAASDNNAFLFFEGAWNVLQPKIADGTFVIKNSSEAVALQGKAKLTRDEMGK
                     IIGQVTTNWDFNEAKNKAEAHLTAAQAADKGNVFILAPNDGTARAIADAFAADPAVTS
                     YKVTGQDAEKASVQYIIDGKQSMTVFKDVRTLVKDSISMAVSLLEGKEPQTTGSYNNG
                     KIDVKAKQTEVIVVKADNVKSALIDSGYYPASDFTNLK"
     gene            complement(28126..29196)
                     /locus_tag="PKI50_05585"
     CDS             complement(28126..29196)
                     /locus_tag="PKI50_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNT03217.1"
                     /translation="MAKENKVKNSIIVVLFTVFLMLILSIASILNRTDRGFFQSNKNI
                     EEREKIKIGFSLGTLKEERWIKDRDILIAKAREFGADIIVQNANNDDQDQMKQVRYLL
                     EQNIDVLIIVPNDLNKAAHAVELAKRQGIPVISYDRLVLNSNVDLYVTFDNVKVGELM
                     AERITDSMKGENLLIVNGAKTDNNTKMIKQGYDKILGPLEAEGKIKIIAEEWAADWLK
                     EYAFKVVDDMLQKGIRIDAVISGNDSLAEGVIEALSEHRLAGKVSVTGQDADLTACQR
                     IVEGMQLMTVYKPIEKLAEAAVEMAVRLVKKEPLNVQDYINDGTYEVKYYVLTPIAVD
                     KENIDETIIRDGFHLEDEVYRH"
     gene            complement(29189..30799)
                     /locus_tag="PKI50_05590"
     CDS             complement(29189..30799)
                     /locus_tag="PKI50_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT03218.1"
                     /translation="MTKLLIADDEYLVLDSLKMIIRKNMKDIDIVATASTGREAIEKA
                     IELKPDIIFMDIHMPGIDGIEAIRQIKAVNSSILFVIITAYEFFDYAKEAINLGVSEY
                     ILKPIEKSKVIDTLNNLCNTIKQNRRLLLKEAELKERINRIIPFIESQFVSYQLFTSG
                     TISDIEFYEEIFNINLRQGYALTVLLKNFEGKIKEDNLKLSLEKQNFYEKFSLELKRI
                     CPCLIGNPLLDRITAFIPSEKGIDSYEIRNESIKIANRIIDRIKPGTGIEYTIGIGRS
                     YEAGSFNKSCNEAYIAASVSNGHAVMHYEDITPSNNIPDTYPAQKEAIFANRMLTGNL
                     QGAGEAFEEIYLWLITNYYEDMDKIKSKLIDLLFVIEKALPYKASSFSKSKQSYIFSI
                     LKTNSKEVLKRQFMDIISQLSEEIQEQIRSERDGIIPKVLEYLNNNYHKEINLADAAR
                     YVNLSYHYFSKIFKDEAGKNFSDYLTELRIEKSMGFLKDHKISIKEVCFKIGYNDPNY
                     YCKIFKKITGMTPTEYRAVINIRGDDIG"
     gene            complement(30777..32318)
                     /locus_tag="PKI50_05595"
     CDS             complement(30777..32318)
                     /locus_tag="PKI50_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNT03219.1"
                     /translation="MNPGLLTFIGIRKKLIMYFLLTILLLGVISIFSYYNARVVLRNT
                     NSIITDYSYLNSLNNDVNSLMTELEKYLMTASSESLLNYYTHYNILQETTRKIPRTAE
                     YNNELLLMKDIGNMIDELLIETDNAIQAKRGRISSRYISHFQRSNRISDYIKLYNNKI
                     LDSKLKSGTEKYENINKTMNFISYMNLLIIIAALLANIYIAVVSTYRLTKPISELSYS
                     AEKIAEGDFDIELRQTHTGDETDILAGAFIKMVNSIKAYINELKRQAEVEKRLKEQEM
                     QNLKMMSHLKEAELKFLQSQINPHFLFNTLNAAAQLAAIEGADKSSEFIVSISNLFRY
                     NLKNIDELTTLEDEIQYVKYYMQILKTRFGSRIDFYTDIDETTFDIMIPRIVIQPIVE
                     NAFIHGLQNLERHGEIHLNVRREGDCIMVEVIDNGIGMDSACIEEILRFEEDGSKYRN
                     RLTGIGMSNVLYRLQLFFNIEDPRKLLSIESEIGHGTKVTLKLPIMKGARREGEDTYD
                     KASDS"
     gene            complement(32354..33355)
                     /locus_tag="PKI50_05600"
     CDS             complement(32354..33355)
                     /locus_tag="PKI50_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNT03220.1"
                     /translation="MKILRNLLRFLYVHAILILTLMVIAASAVLFWLQREDIKTYISK
                     PEYHFYFIAQNSVDPFWNEVMQGVQNSAEDNNVVVEYNAPRFSNPEEELQFMDIAIIS
                     KVDGIITHVGGTGFTEMIDKAYDNGIPVITVENDDSESKRYAFAGTNSFELGREAARL
                     LIEATNADAKVAVILNGDLEQEAIEYNLKMNGFISTLKEYPNMKIIRTYTSKMGILSA
                     EEITQSIIDSDEGINAIFTAGSADTLGSAQLIVDRSKVGKIVLVGYGNSEEILRYIDK
                     EIIYGTVASDPYKMGYESVRALVDVKNGKSVPSFIDAQVKVIAKKNLSSYKKAYKLD"
     gene            complement(33511..34050)
                     /locus_tag="PKI50_05605"
     CDS             complement(33511..34050)
                     /locus_tag="PKI50_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03221.1"
                     /translation="MKRVISFALVLLIVVPLLGCVTDNSNDKASDKAAQNEIHIRVGQ
                     QDINYTLTSRPFTSKEDFKKAYLEIDREKLIYVPRGTKITLDMKEQDVKASVKAYWIN
                     REGAPNYNDSFGAPLDMDVEVEKIGDSKYEFTAERLLQAGLSSHYEENEKITAGYVAE
                     LQKNGETRYCFFIIQIDKN"
     gene            complement(34047..34622)
                     /locus_tag="PKI50_05610"
     CDS             complement(34047..34622)
                     /locus_tag="PKI50_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT03222.1"
                     /translation="MLDAGCGEGYYICRLKNSLTDFCDCIKTDIYGIDVPKAAIHYAS
                     GRDKGIRFAVASNYHIPVLKGTLDCILCSFAPRDEGEFRRILKPKGKLIVASPGARHL
                     YSIREALYESPEEIGQKGTVGEGFTLLEKKKVNYEIILKSKQDILNLFTMTPYSRHAE
                     TSALEKLSEFATEVDINIFVYSARPAGRSFK"
     gene            complement(34644..34922)
                     /locus_tag="PKI50_05615"
     CDS             complement(34644..34922)
                     /locus_tag="PKI50_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03223.1"
                     /translation="MARIMMHSIFQCPNCFKALKQGERQYFCPTGHSFEIAKKGYVNL
                     LLPSHTGAGTPGDSKEMLQSRREFLDKGYYEGFSNMLNVIVLSALLID"
     gene            complement(34910..35230)
                     /locus_tag="PKI50_05620"
     CDS             complement(34910..35230)
                     /locus_tag="PKI50_05620"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNT03224.1"
                     /translation="MSDKREGFFKRVYEIVGRIPEGKVAAYGGIARMLGCSGGARTVG
                     WAMRSAPEDMKLPCHRVVKATGELSPGHVFGDPEIQRAMLEAEGITFRADGTIDMERH
                     LWQG"
     gene            complement(35245..35634)
                     /locus_tag="PKI50_05625"
     CDS             complement(35245..35634)
                     /locus_tag="PKI50_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016767347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT03225.1"
                     /translation="MKIEHIAIWTKDIERLKMFYTNYFDGIAGEKYINPKKSFESYFI
                     KFDSGARLELMQMSGSPLDSNDTINQHIGLTHIAVSTGSAEKVNSLTDELRKAGYTVV
                     SEPRHTGDGYYESCILDPDGNRLEITV"
     gene            complement(35847..36236)
                     /locus_tag="PKI50_05630"
     CDS             complement(35847..36236)
                     /locus_tag="PKI50_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT03226.1"
                     /translation="MTEKALTMKLMEGLYGVCRLDKDEAIPEWVSQCSFYSVTKTLDE
                     LSVVCSQDNIPDKIKCEKDWRILRVEGPLDFSLIGILSSISTTLANKGISIFAISTYD
                     TDYILVKDKNIDTAINALSAEKYEIIR"
     gene            complement(36304..36810)
                     /locus_tag="PKI50_05635"
     CDS             complement(36304..36810)
                     /locus_tag="PKI50_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03227.1"
                     /translation="MKHKAYKAAVWAGAFLFLLSSIVYANSSWHWVTASPMKVLPFAV
                     VFTILIETAFVTKLGGVSDSRKAFYIICLANLMSFIAPYLERAYRFIPTSGGYSIFAA
                     FNKGPYYIVLAGYLILTVAVELPIVYFLLRKDSLNRKNLIVSILLSNIVTTLLVAVCE
                     RIICVGQW"
     gene            complement(36870..37031)
                     /locus_tag="PKI50_05640"
     CDS             complement(36870..37031)
                     /locus_tag="PKI50_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03228.1"
                     /translation="MAVSLGDIEKVNSLTYELMSADYTVVSEPRYTVDGCYGNCILDP
                     DGNRLELTV"
     gene            complement(37045..37761)
                     /locus_tag="PKI50_05645"
     CDS             complement(37045..37761)
                     /locus_tag="PKI50_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03229.1"
                     /translation="MCKKDKLLLIMILSMAMLIIVSAVCYGNSAEPPSILIIAPDAPD
                     ELEISIGPADIKASMRDKIIERYYVFYSADLRIAKDYNINISTGEDSFEVLLEEPLKT
                     YNNIYTLDLENQTLTPGKSLSRSILLVSLRVILTLIIEAIVFWLFVFRKKNSWLAFLI
                     INLITQGALNIWLNSSVPLASYLVFSLIFAEFFVFIAEIVLFLTLIKEHSRRRTVLYV
                     IAANFLSLFAGGYIITVLPV"
     gene            complement(37824..38063)
                     /locus_tag="PKI50_05650"
     CDS             complement(37824..38063)
                     /locus_tag="PKI50_05650"
                     /inference="COORDINATES: protein motif:HMM:NF022308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2651 family protein"
                     /protein_id="HNT03230.1"
                     /translation="MEFLMALLIFPLLSFLFGIIGQVLIRKIFIVAGITFALWSIAAF
                     TVFNSSFLVWVFVYTVLSALGASIVHFGKDLKKRS"
     gene            complement(38247..39407)
                     /locus_tag="PKI50_05655"
     CDS             complement(38247..39407)
                     /locus_tag="PKI50_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03231.1"
                     /translation="MMIVKKPNVLLSTILAIFLIMAFVSGCNDTGKSHKEITALTDEE
                     LSYFNGDEFFNGEYLNIRNQFLSSTYERPEKIDIFQLFYCGSGIEEISTEEERAAVVA
                     YNNWDMEPDCACMKISRSNMESVLGKYMGLTLADTDEIGLENFTYLEEYDAYYYYHGD
                     TNYRMEIIFSGGERDGDIVRLFYDDVFFSDGKKILTLQEKDGSYLFVSNVYCEKPIED
                     GNKADVGLDQRSDGSIFSLSIPGTGTLSLNAYPVDFPFSLDLTEKSRKSFRAESFGNV
                     TVVESDNLQVTYLNPDEGVHTIITIRTVKEGCSSAGVTIGDTEETLLEHWTEKPLKKM
                     DSISYDDEVWFGKCDLAYAHTPEEGTKSVVFLIEDGLVSGIEIINGLDGAIY"
     gene            39801..41084
                     /locus_tag="PKI50_05660"
     CDS             39801..41084
                     /locus_tag="PKI50_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HNT03232.1"
                     /translation="MNLNEKIKILSDSAKYDVSCSSSGSSRKNKKGGIGNAAPSGICH
                     SWTPDGRCISLLKILLTNYCIYDCKYCVNRSSNDVPRAAFTIDELVNLTINFYRRNYI
                     EGLFLSSAVCVSPDHTMELLLKSVKRLREEVNFNGYIHVKAIPGASNKLIEETGKYVD
                     RMSVNIELPSGESLKLLAPQKTKESIIKPMGLIKSGIIQYQEEKLKFKSTPQFVPGGQ
                     STQLIVGATKDNDLKILKLSESLYSSYKLKRVYYSAYVPVSNHPNLPAISGPPLLREH
                     RLYQADWLLRFYGFNSGELLNEENPNFDELLDPKSGWAIRNLDKFPVEVNKADYYTLL
                     RVPGIGVKSAQRIVQGRRVSMLDFDDLKKLGIVLKRAKYFITCKGRYFPQKSIPTNAV
                     SIKNRLLLEDNIKNRGNIEQLSIFSLFPGITDGEF"
     gene            41084..41821
                     /locus_tag="PKI50_05665"
     CDS             41084..41821
                     /locus_tag="PKI50_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNT03233.1"
                     /translation="MLYYIYDGSFDGLLTAIYDAYYNKDFPEQIVPQDSFTDNFLINK
                     VYIRTEEEKSRKVHDAVLQKISRRAMAGIMYAYLSEDENAGISILEYLRFGFKVGRTV
                     DSYLADDRVNNINRLVTKVAREKHLLAGIIRFRLLHNNIYYAPIEPQYNTLCLLGKHF
                     ADRMSDQIFVIHDVKRSYGVFYDKTKWFMSDIEMNKDIVLHENELFYQELWKRYFDNI
                     TIRSRINPKLQSNHMPKKYWKYLIEMK"
     gene            complement(41822..42544)
                     /locus_tag="PKI50_05670"
     CDS             complement(41822..42544)
                     /locus_tag="PKI50_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HNT03234.1"
                     /translation="MSRFIIEQPFWDIFPECEIGVLLINDIDNTEEGCEKLRSDIIEF
                     LEQSNQEARKYLTEPVLSQNRVISVWRDAFSKFKTKKGARSSIEALLKRIEKGNEVGV
                     INPLVDIYNGISLRFALPCGMEDVDTFKGDLRLTVTNGGDPFLALGDEEIDYTLPGEI
                     CYLDDEGAVCRCWNWRDGQRTMLTEDTKNAIAVIESVDPLRHKDLEAALDTLSAWIEK
                     VRIGKVNTKVIMGKNNSTLTTR"
     gene            complement(42850..45633)
                     /locus_tag="PKI50_05675"
     CDS             complement(42850..45633)
                     /locus_tag="PKI50_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT03235.1"
                     /translation="MVIDFERPLMLFLIPLAIFFVFFTARKLRKMYRLRRRLILFLRN
                     SVFVLLILALTGVNIKWIVDTTTTIFVIDASDSMREYRTTSEDFVRKALEKKGSKDQT
                     GVVAFGDNSLIESFVSGDSVFNKIETEPRGIYTDIEGALTTSVSLLPQDSKKRIVLIT
                     DGEENEGNSSKLASSLIGQDIDFKVYRINREIGKEAAVESVTVPKSLRVGEEFNIIVT
                     VESTVQTEAKLTLISGKDKAAEEKVALQKGSNRFVFRDTAETGGFKSYKALLEPDTDT
                     ESRNNEASTFTNVLDRPRILIVEDVAGEADEVARMLEATGMNYVRVNAAGVPGTLEGL
                     SAYKSIITCNVSAENLSDGFLNSLDSYVKDMGGGFIAAGGENSFALGGYYKTPLEKVL
                     PVHMELKGRKEIPDMSIVLVIDKSGSMTEGRGGIMKLDLAKEAAARTLESLRPKDQIG
                     VLTFDDTNYWVVPTRKADDAEKIRDDIGTIRPGGGTSIIPALQEGYDSIKKTDTKIKH
                     IILLTDGQAERTGYTELVEKLAGDNITVSTVAVGQGSDVQLLQDIAKGANGRFYYTDE
                     FANIPRIFAKETFMAARAYLNNREFVPAITNTHPVLEGAAEEGLPALLGYVASSPKSA
                     SRVVLASDQEDPILTVWQYGLGKTAAWNSDINGRWSRNYVGWENNIKLWQNLINWTIE
                     KYREDDITVEAEVQGGKGTITLNNKADKGEFESKASIVSPSLESSEITLYPVAPGQYR
                     GDFEITETGSYIIKALQQKDGETINAASTGISVQYSPEYRIQPERGYLDRLIKEAGGT
                     LINTPEDVYKGEIKDIFGTVDLTPALLILALILYMLDIALRRLNLPLAAVEEKLSAAR
                     ARLIPQIKRSAKVKRPISTMDEALKAAEKKVHMPPQAEGKPEAKEKEAKPEKPKTENL
                     DTSTLLKKKKSRE"
     gene            complement(45623..>45808)
                     /locus_tag="PKI50_05680"
     CDS             complement(45623..>45808)
                     /locus_tag="PKI50_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03236.1"
                     /translation="SSFAVNFPTESESVNSKQEVDISQEASEAGNTSGGTRLQELFLG
                     LMLMAAAIEWVVYIRGY"
BASE COUNT        12178 a        10907 c         8110 g        14613 t
ORIGIN      
        1 aaaaggatta atctctgtca atatctgtct atctgtcgtg cctggcacct attcttcacc
       61 tattcttcca tcgtcacctc ttaacgataa tttcataggt tcttgattga acctgacttc
      121 tttcaccgat aaatacggtc aatctcgtgg ttccttcctt attgcctgta actgatgcac
      181 tgtaacccga tataatgacg gtcttttccg caacttcacc aagtatccag ttagtatacc
      241 ctcccacaat atcgccggat atgcttgcta tactgttatc atccaccttc catgtcgtaa
      301 tcttttctga atttgaaaaa tcactgccgc ggacgataac attaatgttt attgtctgtc
      361 cggcagaaac aataatgggt gattgctgga ccagcctgaa ttcgtccagt tgatatttat
      421 caatattctc atacattctt ttggcaagaa taattgcctg ctccctggtc aaagcacctt
      481 taggtgtcat ttgattattg gacccctgaa tgattccacg gtaattgata tagtaaactg
      541 catccaccgc ccagtttgag atattagtgt gatcctcaaa cattttgggg gaagaaacat
      601 gtgtatctaa tcccggtttt attttattca taagtctgaa aaacatcaat gaagcctgtt
      661 cacgggttaa agagtccttc ggcataaaca tattatttcc tgctcccgta acaattccaa
      721 gctcgcaggc tttcaaaaca tagagattaa cagtatcgtt gaagggattt gcctcgggga
      781 gtggcgcttc atgtttcgtc aggatttcat aaagtcgaac caccagttca ctaaaatcct
      841 cccgtgttat attttcggtg tagctaccca gcaagttatt agaggtaagg cccaaagttt
      901 tggcttcttc cacatctgtc tgtgcccagg agctgggata attacctgca taggtatcca
      961 atttttcgtt ccatcccagt tttgcgatcc atgcgtcata tgaataggat tttgtaggat
     1021 tgtgaagtcc ttttacgtcc ttatcattag aattggtata cccggctact atatatccat
     1081 ccttctctgc taccaagctt tttggttcat ccgcattgct tccgcccaag cttttttccc
     1141 atatcaatat accctcggag gacagaccgg tcaaccagaa atccgagttc ccttttgaat
     1201 cgctgatatc tccgtctctg gattcactgc gtgcaagtat agcataatag ccgtcacttg
     1261 tctgtttaat gcttatcaca tgctcattcc ggctcccgcc aagatagttt gcccactggg
     1321 gattcttacc gctatcaaat ttaacaacca taataatcgg actggtgtta tatgtgtcag
     1381 aatacagagc catggggaaa ctccgtttcg tgacgctctt atataggtca atcagttccc
     1441 ggtgatattg gttcaccgac accgcccaaa caaatccgcc atctgcggtt ccttcaatgc
     1501 tttgttcttc ctttacagag ggctcctgcc agagaatgtc cccatcctgt gtgaaccgga
     1561 caataaaatc accggcagag ataaaatccc cgttattcag ctgcgctata tcatataagt
     1621 aatatccggg cagggttttt tgccacctta tacttccctg aaaatccagc ctaaccgcat
     1681 atgcattata aaaattattt tttccgcctt gaatccccat caggtaccca tcgtcgataa
     1741 tgtgaagact tatcacttta cttgcttccg tgtccacatc ctgcaatact ttttcccata
     1801 gaatatcacc cttggcagac agttttacca tccagaccac aaagtctgag gacggattgt
     1861 tttgattact gacaacatct ccgtttttcg aaaatgtcat ccctgccagt ataaaggttc
     1921 catcactgtg ctgccgtaca gcaaacccca tatcacctgc ggaaccacca agggtttgat
     1981 gccatacggg ctgtccgttg gaatcaaatt ttacaaccag caaatcatca tcttcacgaa
     2041 agccggagcc gggctcattc acccgctgct ttgttgttat atctccatcc ggagaattgg
     2101 ttgtgcctat agccagataa ccaccgtctg aagtctgtat cacttcagat atttcggtgt
     2161 tatcactgcc gccgaaagcc ttttgccact gaatctgagg cgactggtga acctgagcat
     2221 gaacagttaa aggaattaaa gttgttgtca tgtaaactaa agccaataac aaaagtttta
     2281 tttttacttt catggttaac caccctttca tagtatgatt ttattcatat tatctgtgat
     2341 atttttcaaa catcctgttg gccagtataa aagcttgctc tcttgttgcg gttcctttgg
     2401 gattaaaacc ggttcccgta cccaggataa ccttttcgcc attcatcact gtaaccccct
     2461 taaccgccca gtcagaaatc tcgttattat catagaacct gatttcatgt gatgttttta
     2521 actgaatatc cggcctcgaa ggcaggattg cttccagaat cctataaaac atcaccgcaa
     2581 tctgttccct tgatatgttt tcatttggag caaaacggcc atttcctaca ccctgaataa
     2641 ttttcaaatt gtatgatttt aagacgctga tatcatttgt atccgaaaaa ggagttacca
     2701 tatcataggt tgctttccgg ccactaagct cttcatacag cttaacagcc agtgtgcaaa
     2761 actcctgcct ggttatatca ttttgatact gactgagcaa gctctcatcc accaggttgt
     2821 tccgaattgc tctggttact tcttcctcag cccagaaact tggtttatcg gaaatatcac
     2881 cttcagcatc ttcttccgga acttcatacc gaataacata aggctgatat tccgttcccg
     2941 gccctgacat cgaataaccc ggtttcaagc acagtgcagg tctgacgcca ttagcctggt
     3001 atgcattgaa cccgtgtgga tcacttaatt tgtttgatgg aattgtcatg acagacgctg
     3061 cgtagtcata atcgggtgtc cgcagccaat aggtattgat ttgccagcca ttgggggcag
     3121 tataccaggg tgctgttcga ttcaattcgt tttcttcgat ccaggctttt acttcatcaa
     3181 cagacaacaa aaatattttg tggcttgaca atttataata ggcattacaa ttgtctccat
     3241 tgagattcca tggctgattc ccaccatcct tcaccggcaa atcaaactca ctcagatatg
     3301 tacgaagcga tattgtctgt attgccgcca tttccatatc atgaaagttt gtaagaaaac
     3361 ccggctcatc cgcatagctc acatcctctg ccaatatact gctcttaccg tttacaaagg
     3421 ttgaatacga tacccccata gtattggcag catcagggtt ttgatgtgaa taattcactg
     3481 tctgttcctc gctgttcaac cattcacata agtttgcctt cgtccaatag ttgctgcctt
     3541 ccgccactct ggtattgtac ttgctgtctc ggccttctgc cctgtctccg ctggcatcaa
     3601 aagcttttac cgtaataata tcctttgcaa acaagatact cccgttttca tcattatgta
     3661 caaccatcca tacaataggt ttgtctttat acatgccaaa ctgcacataa cttcctatcg
     3721 gcagcgaacc cgaaaaagtt attgcatata cttctagcag accgcccaaa attattatca
     3781 tactcatggc cagggtaaaa actattttcc cccaatgctt atacctgata aacaaattta
     3841 tgcatttcat tttaatcacc tcatacagta ttttatatac tatgtaaaac cggttcacta
     3901 tcaatcatct gtgttagttt aattataaca agacaaacct tataacagtc tgcagcctat
     3961 attttgtcaa tgacaaaatg agtcacaaat catgacataa ttctgcatga atcgcatgtt
     4021 aactgccatt aaaccttgtt gttagataat ctctgtggta tatttataca gaggtgattt
     4081 tcttggaatt tcatgacaaa ttaaactttt tgatggatat aacaaaaaca acaaacatca
     4141 gcctggcaag ggcctcatca atggatcctt cctatatcag ccggctgcgt aacggaaaaa
     4201 ggaaacctgt aaaaaatgcc tcttatatga tagtctttgc ccgttttttt tccaaaagga
     4261 tacactcaga aaaacaaatg gatgttttta gggaggtatt gctgccatac accatgtttc
     4321 ctgaaagcga gagtgcttta acggatttcc tgcatcattg gcttgtgaat aatgacttta
     4381 aaaacgggca cctgaacctg cctgttctgc ataggcttac tgatgcagaa ccaagaggcg
     4441 gcttcaataa aaatttgaat gaaaatgtga agccattttt caaaaccgcc tgcggtcttt
     4501 accagggtat acaggaaagg cggctggctg cggttcactt tctgagcaca attctcaacc
     4561 agaattctcc gagaaccctg ttttttttca gtgatgaaga tatgagctgg atgactggcg
     4621 atccggtatt ctatcaaaag tggtctcatt tgatgggtca aatcatcgcc agaggaaatc
     4681 ggataaaaat cattcatgat ttgacccgtg gcctcgatga aatactgtcc tcaatacagc
     4741 tctggattcc cctctatttg tccggagcca tcgaacctta ttattatccc aggaaacgag
     4801 acggcatata taaaagatca ctgtacattg cccccggctt gtgtgcagtt tcttccgact
     4861 ctatcggaac gatgacggct gatgaatcca ccgcggtttt tcaccaggac cttagaatgg
     4921 tagaagccct tgagaatgaa ttccatcatt atctcaacct atgcagaccc cttatgagca
     4981 ttttcactcc ggataagcat cagaattatc tgtcccttct tgaatctttt gaaagggagt
     5041 acgctcctac catattcaaa accccgcatc tttccatgct ctccatgtcc gaatccttaa
     5101 tggaatccat tcttttacga tattgtaaga caagctataa taaaaacaga agtttttaca
     5161 ataaaagatt ggaaaatttc cgaaaaacat tgaaaagaca cagctttacg gaaattttca
     5221 gtattccgga aaaagaagct ctgctggcag gcaaagctag aatcaacctg ccaaatccct
     5281 ttgaatccat tgaaggtttc tacacccctg atgattaccg tcgtcattta gatcatgtgg
     5341 cagtcctgac taagcactat gacaactaca atgtctgcat tgaaaaaaac gaatgcagtc
     5401 tgggatacac tctttatgcg aaagaaggta taggcgttat agtagctaaa accaaaaccc
     5461 ctgctgttgt tctttttatt caggaaagca atttatttgc cgggttttgg gatcttctga
     5521 ttgaaatgaa agagcatggt gccagacacg atgtttagac agttatactc atctgctttg
     5581 gaatacataa cctaccctct tgtattttac attaaaaata cttgcttaat aattattgat
     5641 ttgatgtatt atttcctgaa gctttttttc aatagtggct ggcactcgtt cgacctgcat
     5701 aaaagccttc cctgatagca ttaccgacct ttccaacctg ctgacagtca ccgattatcc
     5761 gagtctttgt atggaggtta tgataataaa aaatacgacc gcagtcgtat tttttatatt
     5821 attttaagtt tttgacaaac ttttcgatat ttgcgttctt tctcatttct tcgagaagct
     5881 tctcatattc cgagcccttc ttctcataaa gcagtttccc cttaagcatt tcttttacat
     5941 cctcaaattt gtcccgctta ctgtcctcaa cttttataat gtgaaaacca aagtcagttt
     6001 gaacgatttc actgatctct ccgggcttta gtgcaaaagc cgctttctca aaaggctcca
     6061 ccatatctcc gtgcctgaag tagtcaagat ctccgctgtt ttcctttgca ctgggatcct
     6121 ttgaatactg ctttgccaac tcggcaaaat tctctccggc ttttaccctt gtaagcattt
     6181 tctctgcttc ttccttggta tccagcagta tatggcttgc cttgacgttc ataaattcat
     6241 cctgatgggt atcataataa ttttttagct cttcatcagt aactgttata tcctcagtaa
     6301 gcttttcaac aagcttattt atggtaagct cctttttcac cgattccttg aagtattcaa
     6361 gatcaatacc ctgccccttc agaaattcat ttaacttttc ctctgaatcg aagtattttc
     6421 tggcattttc aatttctgcg ttcacttcgt catcggtaac ggtaatccca agctccgcag
     6481 ctttttccat ctgaagcttt tcatcaatca gcatctccag aagcttttcc ctggcaacat
     6541 cgccgaactt ccttccttca acctcctggt cccacacttc agtaccaaac tgctgctcgt
     6601 actgtgtctt gaatatatca aacattttat taaattcttc ttttaaaatt tctttaccgg
     6661 aaacctttgc aactacagtt ttattatcaa cttcgggatt tactctgacc atattgcagg
     6721 cagtcggaaa tactgtcagc aatacaagtg ccaaagatac cagaagcagc ctgcattttt
     6781 tattaaacaa tttacatcgc tcccctactt gtttttagtc aaggttaatt ataccctata
     6841 tcagccgttc tgaaaactac taatttttcc tagaaattct ttcaggaatt tgatatactc
     6901 atccggtctg tcttcatcaa cttttatggt gaaatatggc tggccgcttg cggtaaaaag
     6961 cagcctgtcg atgtattccc ctgctaccct tatagctgtc tcaggcttaa tgctcctgtc
     7021 gctgctgaat ttcaccaata tgctgttttt cttttgagtt atggatacta cattacactt
     7081 acctgctaaa tgctttatat aggatatatc cagcagattc ctgactacct gcggtaaatc
     7141 tccgaacctg tcctctattt cctcttcaat gtcatagatg tcctcctggc ttccaatgta
     7201 tgcaatcttc ttataaatct caattttttg attctcatcg gatatatagc tttccggaat
     7261 aaatgcgcta atcttaagct ctattgatgt atcaacggtc tcggtgacct cttcgccctt
     7321 aagctccttt accgtatcct caagcagctt tatataaagg tcatagccta tggcctccaa
     7381 atgcccatgc tgctcccggc cgagcaagct gcctgcccct ctgatttcca aatccctcat
     7441 ggctatttta aaaccggaac cgaattcggt gaactccctt atggcttgaa gccttttttc
     7501 cgcaacttca ttcagcactt tatccttctg atatgtcaga tatgcataag caagcctgtt
     7561 ggaacgccca acccttcccc tgagctgata aagctgtgat aatcccattt tatccgcatc
     7621 acttattatt atggtgttta cattgggtat atccaggcct gcttctataa tagttgtaca
     7681 gaccagtacg tcgtattcat tattataaaa atccagcatg gtgtcctcaa gcatcttctc
     7741 ttccatctgc ccatgggcta ccgccaccct tgcatcgggc agcatcgcct taagctttgc
     7801 ggcaattttc tgtatgcttc ttacacgatt gtgcaaataa tatacctggc cccctcttgc
     7861 tatttctttg gcaattgcgt ctttgattac ctcttcgtta tactccatta cataggtctg
     7921 aacgggatac ctttcttccg gaggctcttc aattatgctt atgtccctta tgcctgaaag
     7981 ggacatgtgc agcgttcttg gaatgggagt cgcagtcagc gtcagcacat ccacattctt
     8041 tttcaggctc tttatctttt ccttatgggc aacgccaaac ctctgctctt catcaactat
     8101 caaaagcccc aaatccctga actccacgtc attctgcagc agcctgtggg ttccgattat
     8161 tatatccaca ttgccgttct ttatattttg gattatcctg ctttgctcag catgagtctt
     8221 gaatctggat aaaacgtcta tattcacagg gaagttggca aacctttgaa tacaggtatt
     8281 ataatgctgc tgcgccagta ttgtcgtagg aaccaggatc gccgcctgct tgttgtccat
     8341 gacgcattta aatatcgccc ttagagcaac ctcagtctta ccgtatccca catccccgca
     8401 caacagccta tccattactc ttgaatcttc catgttcttc ttgatttctt caatgcatct
     8461 cagctgatcc tgagtctcct gatattggaa catatcctca aattctttct gccatctggt
     8521 atcctctgaa aactcgaagc ctaccgcctg ctgcctttct gcataaagct tcagaaggtc
     8581 cattgccata gcttctatgg ccttcttggc ttttgcctta acctttaccc actctgttcc
     8641 gcccagtttg ttcagcttgg ggcccttgtc ctccgctcct atatatttct gaatcatatc
     8701 cagctggtcc gtgggtatat aaagcttgtc attccccgaa tacctgatat gaaggtaatc
     8761 tctggtagtg ttatctatct tcagtttctc tatcccaatg tactgtccta taccgtgatt
     8821 ctcgtgaacg acaaaatccc ccgctttgag atcggtaaac acatttatgg ctttgccttt
     8881 tttcttttca atcttcttcc tgctctttga gccgaaaggc tctctgtcgc ttatgacggc
     8941 atactttatc gatggaaaat caaatccgct gttcagtatt ccgggaagaa tgattacatg
     9001 cccttcctta agctcaatat caagctcatc cttgtatact gcatcaatat ccctgtctct
     9061 aaggtctgaa gccagcctca agcccctgtc ccttgagcct gaaagtatca gaaccctgta
     9121 tctattcttc ctccatacgg caatctcttc cataagcatg gaaagcttgc cgtggaaggg
     9181 atgcattgac cggctggtaa tgcttatggt tttttgagga ataaaatcac tgctgctttt
     9241 atacaatgaa tccaggcaaa ctgtcttttt ctcccggatc ttcggaacaa catcttcgta
     9301 agtaaataat gcttcaaatt gccccggtaa aacttcaccc ttctccagaa gctgattaaa
     9361 atgctccatg aattccgtcc ctatggcctc gcagcgctgt tttatcctgt taggctcatc
     9421 tatgaaaata acacagtccc ccatgtaatc tataagactg taattcttat ctgaaaaata
     9481 cggcgaatat ttttctattc cctcaaagtg tataccctgc ctcatttttt caatatcctc
     9541 ggatatcttt tcccttaccc tgatatcgga ggattttctc ctgcgctcat ttcctgatgc
     9601 cctaagcctt gcagaaagct ctgactgaag cttttccgcc ccctcaagga attcctcctc
     9661 cttaacgata aattctctgg caggcgtaat tttgactttt tttgtcttgc ttatagagcg
     9721 ctgggtcagc acgtcaaagt atcggatgga atctatctca tcatcaaaaa gttcaattct
     9781 tactgcgttc tggtgtatgg gagaaaagaa gtcaattata cctcctctta ttgagaactg
     9841 tcctttgccc tctaccattt caattcgctc atatcctgct tttatgaaat attccttgat
     9901 ctcatccagt gagacggttt gccccactga aagttccctg gtcattgatg caaaaagctc
     9961 aggagacact gtccttgtga gcagtgcctc tatggaagtg cagacaatgc aatttttacc
    10021 gctgatgata tcgtcatatg cccttaatcg gttctgcagg atttcattgc ttctggcatc
    10081 aatcctgtga aatactaatt ccctgccggg cattagcgcc gccatatccg gttcaaaaaa
    10141 agaaatgtcc tcataaatct ttctggaaag taaatcattg taagttatga aaagacatgc
    10201 ctttccaaaa tgcctgcaaa caaatgcggc tgccgcagcc ttctgagtat cggaaatacc
    10261 atagacttct actgtattca gatttgcttc aagagatgaa agcagttcgt tgaactcttc
    10321 catttcataa agaggcttaa gcacgttgcc aatgctcaag ttatcacctc ctgagccaga
    10381 atcaataatg tcagaaaaaa aaggcttctg ttgaagcgtt gccttcattt ttccctatta
    10441 taccagttca ttgccgagtt tattccattt tttattattt cctcgatagc cagagacgaa
    10501 ttcttgacag cctcttccat caggggtatt tcctcatctg aaaacctgct caatacataa
    10561 tcagccaaat ccataaaatc aggctgtttg ccaataccaa ccctaatcct ggggaactgg
    10621 tccgtttgta ataaatatat tatcgacttc attccgttat gagtgcctga gcttccttca
    10681 gtccttatcc tgattcttcc gatatcaata tccacatcat catataccac tatgagcttg
    10741 gatatgtcac acttgtacca gttaacaagc tcatatatgc tttctccgct caggttcata
    10801 aatgtctggg gctttgccag tacaagcttt tcctctccta tcatgccttc tcccaataat
    10861 gccttgtgct ttatcctgct gatgctgata ttatggacag aagcaaaata atccagtacc
    10921 ataaagccca cattatgcct tgtatattta tatttgtctc cgggatttcc cagtcctgca
    10981 atcagataca taataacctc cgcaatgatt gctgcaaaat atttttagtc aaacagcttg
    11041 ctcaaggaca atccttcata gattctcttg atagcttcag caaacaaagg cgcaacagac
    11101 aataccttta tcttgtctac aggtttgccg gcaagaagag gtatcgtatt tgtaacaata
    11161 agctccttta tcactgaatc cctgatgcgt tcaaaggcag gccccgacaa aaccggatgc
    11221 gtacaggcaa catagatatc cctggcgccc atttccttca ttacctttgc agcattcacc
    11281 atggtacctg ccgtatctac catatcgtct attattatac agtttttatt ctcaatatct
    11341 cctatgatat tcataacttc cgacacattc ggtttcggcc ttctcttatc aattattgcc
    11401 agcggagtat caagctttgt ggcaagggat cgcgcccttg tgactcctcc gacatccgga
    11461 gaaaccacaa ctatatcgtc aagcttcttg ttgatgaaat agtttacaat aaggggggct
    11521 gcatacagat gatccacggg gatgtcaaaa aagccctgaa tctgcggtgc atgaagatcc
    11581 attgtaatta ctctgtctgc ccctgcggct tctataaggt tggcaaccag ctttgcggta
    11641 atgggttctc tggacttgga cttcctgtcc tgccttgcat agccgtagaa tggcattact
    11701 gcattaatcc tcccggcgga agctctcttc aatgcatcta tcattattaa gagttccatg
    11761 agattatcat ttacgggagt gcatgtggac tgaaccacaa atacgtctgc gcccctaatg
    11821 gactcattta tacttactcc gatctcccca ttgctaaaag tacttacctg agcatctccc
    11881 acgggtatgc caagattaaa ggctatatcc ttagctaagt ctttattggc attacaattt
    11941 aggactttaa tgctcttccc atgtacattc atttcaacag aaacctccca aaaatattat
    12001 ttgttttttc ttttagttac ccaaccctct ttattttcct gccttgccct gccaatggcc
    12061 aggctgtcct gaggcacatc ttttgtcaca gtagtaccgg ctgcaacata tgtgttttca
    12121 tttaccctta ccggtgcaac caggttgaca ttgcagccga caaaggaatt atcccctaca
    12181 tatgtccggt acttgttctt cccgtcatag ttgacaaata cgactccgca cccaaggttt
    12241 acattgctgc cgacatcgcc gtcgcctaca taggttaggt gtgaagcttt cgaattgtca
    12301 ccaaaggtgg aattcttcat ttcgacaaaa tcaccgactt ttgcatgctt tccaattttg
    12361 ttgcccggtc tcagatatgc aaaaggaccg atagaagtgc cttctcctat agagctttcc
    12421 aacactaccg agttctgtat ctttactcct ctgtctattt ttgcatcctt aatccttgaa
    12481 taagggccta tcacagagtc ctcctgtata accgtattcc cctcaattat gcatccggga
    12541 tatattgtaa catccttctc aactgttacc gaagcgtcta tatatgtact gtccggatca
    12601 attattgtca ctccctccag cataagtttc ttaacaatcc tgcgctgcat aattttgttg
    12661 gcctcgctca gttgatacct gttatttacc cccaatatct cttcggcatt ttttatttta
    12721 aacgccccga tcttaagtcc cttttttctc attattccaa taacatcagt aatatagtat
    12781 tctccctggg tattatcatt tttcaaatac cccagcgctt cctgcaactc ttttccgtca
    12841 aaaaagtata tccccgagtt gatttccttt attagcttag tactgctgtc agcgtcctta
    12901 tcctctacaa ttgcttcaat aagcttctct ccgattctga ttatccttcc ataccctgca
    12961 gggtcatcca tatcagaagt cagtattgtt gcactgcagc cctcagccct gtgcatctca
    13021 cgcatctcta gaagggtatc gggccgaagc agcggagtat ctccatacaa tataagtata
    13081 tcgccgtcat taatgtattt ttctgcctgc ataacagcat ggccggtacc aagctgcctt
    13141 tcctgcataa caaatttaac accggtgact tccttgcgaa cctgttccgc tccgtgtcct
    13201 atcactacaa caggctctgc accggaaacc tcccgggcac agttaattac atgctctacc
    13261 attggtaacc cgcatacttt gtgtaatacc ttgggtactt tagagtacat cctcttgccc
    13321 tctccggcgg caagtattat tggcgtaata ctgtatacat tactcaatct atcacctcaa
    13381 tcactagtta attacaatac tattttatca taacgaaacc tcaggaaaaa gaaccaatag
    13441 gaaatacaat aaaatagctt tagtttagtt tgcttaataa aaggcaaacc attccaaagc
    13501 tgatttacta caaaaatctt aagcttatgt ccattgcctt aacagaattt gtcagcgccc
    13561 ctgcagatat tatgtctact cctgtttctg cgactgctct tacgcttctt tctcccccga
    13621 ttgtaatatt acctgaagct tccagcagca ccttaccgga tgcaatgctt acagcctcct
    13681 tcatcatatc cagtgtcatg ttatccagca taacaatgtc ggcaccggct tcaatggctt
    13741 ctataagctc tcttattgat tccacttcca cttctatttt cagcccatga gagcccttcg
    13801 ctcttgctgc ggcaacggcg ggagttatac cccctgcggc ttttatatga ttatccttta
    13861 ttagtaccat atcagaaaga ttgaacctat ggttgaaacc ccctccggcc tttacggaat
    13921 atttatccag aattctgagc cccggtgcag tttttcgcgt gtccgcaatc ctggttttca
    13981 acccggagat ttcagaaaca tatttggaag tcattgtagc tatccctgac atcctttgga
    14041 aaaaattcaa agccgttctc tcacccttta atatgcttct tgcattaccg cttatttctg
    14101 ctataatatc tcctttgaag gctctctgcc catccttcat gcgggcttgg aagtcaatgg
    14161 ttccgtctac catttcaaat acccgcctgc atacttctat gccgcagata atcccctctt
    14221 cctttgctat aaagttcccc ctgataagag ccgtatccgg tatggtagag tcagttgtaa
    14281 catcccccca gcccaaatcc tcccggagag catttttaat tattctatca atgataatat
    14341 tatccagcac gggacatcct cctactcact gtctgttctg taatggctgc cgcaattttt
    14401 cttccttgca atggctcctt tcagtatgag agatgcaata tatgcaatat taagtgtctc
    14461 catagcactt acgcttccca tttctgcatc ttcaaggctg ccgattattt cattaatccg
    14521 gttcaatgat ttttccatgt ccggtccgtt cctttcaatt ccggcatggg catccatcag
    14581 ggttttaatt tcgtttctga tatccattac attcagagtt tttcctgaag ctccgtaaac
    14641 atttgaaaca tgcacttctt caagcttaat atctttaatg ccggcattaa tatactctgc
    14701 acttcttctt ccaaatacaa gtccctccag cagggagttg cttgccagcc tattagcccc
    14761 gtgtactcct gtatttgctg cttcaccgca ggcatgaagg cactttatat ttgtccttcc
    14821 caacagatcg gtttgtatgc ctcccataaa atagtgctgt acgggagaaa ccggtatata
    14881 gtcctttgta atatccactc cgtgctccag acagctctta taaattgtgg gaaatctctt
    14941 tttcaaatag tcggaatcct tatgggttat atcaagatat acatattcgc tgcctgtttt
    15001 tttcatttcg gaaaatatag cccttgatac aatatccctg ggagcaatct ctcccatttc
    15061 attatattta tacataaatc tctcatgatt cttgttcaat aatatgccgc cttcgcctct
    15121 gaccgcttca gaaatcagga aacggttccc tgtatattca ggtgtataaa aggctgtagg
    15181 gtggaactga acaaattcca tatcggaaag caccgctccc gccctgtatg ccatggaaat
    15241 tccgtccccc gtaaccacct ctgaattggt agtgtttctg tatacctgtc ctacacctcc
    15301 gctggcgcat ataacactgc ggcagtacag caccttcttt gttgcctcgg tatcaattcc
    15361 tgtcgcaacc aatacaccta tgcatcttcc ttctgaaacg aggagatcta tggcaaagca
    15421 attctcttta atcgtgatat tttccctatc cctgcaaacc ctgagaagcg aatcgacaac
    15481 ctccttgcct gtagcatccc caagagcatg tacaattctg aaacgcccgt gccctccttc
    15541 ccttgttctg gtaagggtac cgtccggttc tctgtcaaag ttcgtcccca gcttctcaag
    15601 cagcttgata tcctttggag cttcttcaac aagtacggtt actgcttcct cattgcagtg
    15661 gcctgcaccg gctttgattg tatccataaa atggtactcc ggcatatcat cctcgccgat
    15721 agccgcggca atccctccct gagccagata ggagttgttt tcatccattg tctccttgct
    15781 caggacacat actttatagc cgggattaag attaacagct gaatagagtc cggcaacacc
    15841 gctgccaatt accaccacat cccacttttc gctttcaatt tttcgggtgt caaaacttgt
    15901 taagtatctc agcggcataa atatcactcc ttattttatc ttcagcattc tttccaaaga
    15961 tttcaaagct tttatcctta tatcctcatc aacatgtatt tcgtgctgca tattcagaag
    16021 cgcgctgtga acatcatcca gccttgtctt tttcatattt gtacatatca atcccgtcga
    16081 aaggagataa aaattcttgt ccggattctg cctcttaagc aaatggagaa tcccttcctc
    16141 cgttccgatg attacatctt tttcgggtat ctctcctgca tatttgatta ttgccgatgt
    16201 acttccaagg aagtcggcaa gcttctgaac ctcaggctca cactcaggat gcatcagtat
    16261 ctttgcattg ggatgaagct gtcttatctt ttgcacttcc tcaaccttaa ctcttttgtg
    16321 agttatacaa aagcccttcc agagaattat ttccttgttc tttacttttg atgccacaaa
    16381 actaccaaga tttttatccg gtacaaaaat cacttgtttg ttttccaggc tctctactac
    16441 cttcaaagca tttgaagagg tacagcaaac atcgctctca gccttcacct cggcggagga
    16501 attcacataa cataccaccg ctgcatcagg atacctggct ttcatttctc tgagctttgg
    16561 cgctgtgacc atatctgcca taggacatcc ggcatccctt acaggaagca ggactgtttt
    16621 gtccggtgac agtattttag cactttctgc catgaaatgc actccgcaga atactattac
    16681 gtcactgtcc gtttcagccg catgctggct taaggaaaac gagtccccaa caacgtcggc
    16741 aacatcctga acttcaggca cctgatagtt atgtgccagt attattgcat tcctctcttt
    16801 tttcagcctt tgtatttctt cagccaaatt gttttgcatc tcaacctacc ttctttattt
    16861 tacaattatt tacttaagtg gtgcgggcaa taaaatcaat attttttatt gtatagaaaa
    16921 gttatatttc ccaatacaat aaaaattgat attcttagtt gaatatatca cttattatgc
    16981 cctttttcaa caatgtataa aaaagataga atgaatatca ttctatcttt taaatggatt
    17041 accacctctt atgctttttt ttgtaaggct ttacaggata gaagggcggc cagtaaaacg
    17101 gatatggact gtatttatgt tttttcttat gcttccaata gacagggtat tcatcagact
    17161 catcactgct gtaatcactg tcatcttctt cataccagtc aggcatcctc atatcctcca
    17221 tcccctgcca ttctccatgg tgcatcatgg ggcacatcat cgggcatatc atcggacagc
    17281 catgcatcgg catttccatc tccggaatct ccatatcatg catatccatc tccggcttct
    17341 cagccttggg atattgtttt ttcttgtcgg tcattctttt acccccttcg gattttaata
    17401 ttaagcttta ttaatatata caatgaaggg tatcaaaaaa gaccgcttaa agaaaacatc
    17461 cacctatgct ctaggtggat atctatagtc cgtattactc gttctttact tttccatatt
    17521 cctccaatat tgaggtttgc agtctctctc ttgttgaaga atttatcgga tgagctatat
    17581 ccttaaattc tccgtcggga gtctttctgc tcggcatagc tatgaacaat ccttcctggc
    17641 cttctataac ctttatgtca tgaacaacaa attcattatc aaaggttact gagactactg
    17701 ccttcatctt cccctctgca ttaacctttc tgattcttac gtctgttatt tccattctag
    17761 ttcaccacct tcccgtcgtt cgttttgtca tttcaataat aattcgtcat atattagtga
    17821 tttccttctt tttctaacaa acaattttgc aaataataca ggaaacggta tgtttatcct
    17881 gcttttatct ttaagtatct ttcatgaaat atcaattgcg gtaaatgttt tgcactgaat
    17941 aaattgttta tagtattgtt tgtagtttta cttttttaaa tctatcataa atttttcaat
    18001 ttttagtgag acatggggac gtttcttaaa aattagatgt tacaatgtac tagtaacaaa
    18061 aaattggcta taataataat atggatatta atgatgcttc agaatcttga taataccaaa
    18121 acaaaagttg acagtataag cataagaatg taaaatcaat acgtcaaata atatattaat
    18181 tatataaacc taatcttttt aaggagtgta tcctatgttt tcccttaata atgtaaagaa
    18241 tgttcatttt gtgggtattg gcggtatcag tatgagcggc ctggctcaga tattgctgga
    18301 atcaggctat aacgtaaccg gctccgatat aaacgacagc aatttaataa acaagctccg
    18361 gaaaaacggt gccgaggtta ctatacccca taatccgaaa aatgttgaaa ctgcggaact
    18421 tgtggtttat actgcagcag ttaagcagga caattgtgaa ataatcaggg caaaggaact
    18481 tggaattcct gttattgata gggctacatt ccttggcatg ataatgaaaa actataatta
    18541 cggtattgca gttgcgggct gccatggaaa aacaacaacc acttcaatga tttcaattat
    18601 tattaagaac gcaggtttgg atcctacagc acttattggc ggtgaaattg aagcaattga
    18661 tggaaacgtc agggtaggca agagtgaata cttcataaca gaagcatgtg aatactgtga
    18721 aagcttcctg aaattctatc cctttgtggc tgtcatatta aacatagaaa gggatcactt
    18781 ggactattat aaagatttag agcatatcat atctgctttc aggaagtttg caaagctggt
    18841 accggcggat ggagcccttg ttgtttgcag cgataacgaa aatacactga atgtctctaa
    18901 agatgttgag tgtaaggtac tgacctatgg cataaagaac aaagctgatt tttatgccag
    18961 aaaaataaaa tatgacaaac tgggacaccc ttctttcgaa gtaatgcata gggataaaaa
    19021 gcttggcacc ttctctctta atattccggg agagcataat gtgctgaatg cccttgcagc
    19081 tatagcagca gcctataatg cgggcgtatc catgaatatt gtaaagaaaa gccttaaaga
    19141 gtttaaaggt acttacaggc gctttgacat aaagggcgtc cgaaataata taacaatagt
    19201 tgatgattat gcgcaccatc ctactgaaat caaagccaca ttgcgggctg caaagcaatt
    19261 cccccatcag aagatatggt gtgtattcca acctcatacc tataccagga ccaggacact
    19321 gttcaaggag ttttctgaag ccttctatga cgcagacaat gtgataataa cagatattta
    19381 tgcggcaaga gaaaaggata cgggactggt taatccctct gagttggtgg acagtataaa
    19441 caagtgcagt gcaaatgctg tttatagaaa tgatttccga tgcatagcca atcttatagc
    19501 ccatgaggca aaacccggcg acctgatatt tacaatgggt gcaggtgatg tcttcaagct
    19561 ggggctaatg atacttgatg aaattaaaat ttaactgaca gcttaaaagt ctttgcggct
    19621 taaccgtaaa ggctttttta tccgggcagg gaacatttcc cttgtctctt gttatactat
    19681 attttcagta ttataataag ggagtaaaac aagtccataa atttattcgg aggtcttaat
    19741 ggaagagagt agtattaaca gtacatacca taccattaat cattggaata acttcaaact
    19801 taagcttgtg ttcgaagggg tcatcgtagg agccttttca ggtttggtcg tggtcctgta
    19861 cagatatttg attgaattgg ccggccattt ttcaaaaaaa atatacctga tgcaaaaggg
    19921 aaacaatctg tttattgttt tgtggcttgt atttcttata tttctcggat attttatagg
    19981 agtactcatt aagaaggagc caatgatcag cggcagcgga atacctcagg ttgaaggtgt
    20041 actcctcagg tatttcagta tgaattggtg gaaaatccta ataggaaaga ttgcaggcag
    20101 tatattatca attggggccg gactctctct gggcaatgaa ggaccgtcta ttcaaatagg
    20161 cgcagctgtc gcaaagggat actgtgaaac ttttaagaag aaaaaacttg aagagaaatt
    20221 tcttattaca agcggtgcaa gtgcgggtct tgcggcagcc tttaacgcac ctttatcagg
    20281 agtaatgttt tccctggagg agattcacaa aaatttctct cctgtggtct taacttctgc
    20341 catggcggca tcaattaccg ctgacgtcat ttccaaatac tttttcggaa tgaaacccat
    20401 atttaacttt cagggcatca gtcctttgcc tcttcaaagc tatcactact taatcatcct
    20461 tggcatcata ttaggtttgc ttggagcagt atttaattat tccatgctga aagcaaagga
    20521 tctttattca atgcaaaaat ggcttcctga gcatttgagg cctgttttcc catttataat
    20581 cgccggcgtc cttgggctcc ttctgcctga agtattggga ggaggccatg agttgattgc
    20641 ttcaatggtt aacctaagct attccttgaa tattctgatg ctgatacttg tagtcaagtt
    20701 tatgtttaca atgataagct ccggttcggg tgctcccgga ggagtattgg ttcccctgct
    20761 tgttattggt gcactaatag gcagcatata cggtaatgtg ctttcagatg tattcagttt
    20821 taatcgtgag tatgtaaaaa accttataat ccttgccatg gcaggatatt tcactgcaat
    20881 tgtcagatct cccattaccg gaatcatatt aattattgaa atgacaggat cttttaatca
    20941 tctgctttct gtgtcaattg tatctgctat tgcttatgta acagcagata tactcggttc
    21001 aaagccaata tatgaatcac tgctggacag gatggttaat aaaggaagca gcagattcaa
    21061 aggtgacagc aagacaaaag tgataataga aacagcagta tgcatgggtt ctctcattga
    21121 aggcaaaaaa attaaagagc tgaaattgcc gtcagagtgt ttacttgtag cagttaagcg
    21181 aggtgaacat gagataatcc ccaagggaga tacacaaatt catgcggggg actaccttat
    21241 caccctgaca aacgaggata atgcagcgga aatcagagaa aaactcacag caatagccga
    21301 agttctttct gcatcaattt atgaaaaata agactaactt attctgcgga aagtgcccac
    21361 aggtataagg atgctaccga tccataagga gaatatctct ttctataact ttcaaacttc
    21421 tcctttgata attccttaat gccgtataga ttcatcatcc ccctgcatat cgccagatcc
    21481 ttgaagctta ccacatcagg gcggcagaga gaaaatataa gcagcatctc ggccgtccat
    21541 acacctactc cgtgcagcga ggaaagcttt tttattattt cctcgtcaga tagtgtatga
    21601 agggcagtga agtccacttg cccggacaaa gcagcctccg cagctccttt aatgtatccg
    21661 gccttccttt cggacatacc gcaggcttgt atttccaaaa tttctgcttg tgcaatactc
    21721 tcaggagtaa ttgttccaac cagcgcctcc agcctgttcc atacagtatc cgccgccttg
    21781 ctggaaatct gctggctgac tatacttgat accagtgctg taaaaggatc cggagtaacc
    21841 tcacgtttta tcatgcctat tctgtcaatt gccgctccaa gcttcttatc cttttttttc
    21901 aaatattcta tttccttttg cccatattca aatacatgca ttatttatct ttctccaatt
    21961 ccaacaattt ctttttcatg tccaatcctc ccgcataacc tgtaagtgaa ccgtctgcac
    22021 ctatgaccct atggcacgga ataaatatag gaataggatt gcgattattg gcaaggccca
    22081 ctgcccttgc agccttcggc ctgcctacct tcccagctat ctccccgtat gtggcggtct
    22141 tgccataggg tattccgcac agagcagtcc aaacctgctt cataaagtct gttccggagg
    22201 gttcaagagg taaggagaat tctttcagtt ccccggcaaa ataactcttg agctgccttg
    22261 ctgcttcttt cagcaatggt gtttcacata attgcatatc ctgggacaat tccttgtcgg
    22321 taatgtaaac attagttatt ttgctgtcct tttcggttat tccgattctc cctatcaaat
    22381 aatcataaaa atatgtatac actataaaac acctctgctt tttctgtatt cattcggagt
    22441 ttgtccaacc tgctctttaa agtgcttata gaaattggat aggctgttaa agcctgtcat
    22501 gtaagcaatc tcgataatat ttttatctgc ctgtccaaga agttcggctg atttgcttat
    22561 tctcaattta gtagtataat gtttaggagt aagcccgcta ttttgcctaa aaagtcttgt
    22621 caagtgattt gtgctgactc caagctgctt tgaaatatgg cttatatcta tcggcttgtc
    22681 ataattcgct tcgattatat ccttggcttt attcaccagt tccaagttcg gttcaaaaac
    22741 tgcttcatca ggccggcact ttttacatgg gcgaaatcct tcatcaattg catttactgc
    22801 gttatcaaaa aaaatcacat tcccgcgtac aggggtcttt gccctgcaag aggggcggca
    22861 gaaaatccct gttgtcttta ctccgtataa aaacaggccg tcatagcttt tatcacagtt
    22921 tacaacggct tgccatttct cattatcgga tattcggaag tgttctttca tggattttct
    22981 cctttcgttt tgaaccgata agcatatttt acatgagcca taaatataaa tcttcctcaa
    23041 tttcaccaat caatcttttt tagctttagt tccataaaac gtgtgccttg gatgggatta
    23101 tacacatagg gctcaatatc atagaagcca aaggataaat ataaactctg tgcgctcttc
    23161 atggtcggaa gggtgtccag cctcatatat tgatagttat tattcatggc ttcatcaata
    23221 atcattccga caagcttttt acccaagcct aatccgcggt attcatcgcg tacgtacagc
    23281 cttttcattt cgcagatatc cttggaaatc ttccgaagtg caatacaccc tgcctccctg
    23341 ccctcgacca gcgccaatat cagagccccg tcaggaggct gatacttacc cggcagtgta
    23401 ttaagctctg tttcgaaatc ctgaaaatca agatcaattt ccaatgaacg cgcgtattcc
    23461 aaaaatagct ttcttatctc ttcaatcatt tctccttctg ttacaagaaa gaattccggt
    23521 ttgttcacac ttatcactcc aaaacactct attcattgtt aagttcatac atccggcata
    23581 tatgtatttg atgccgttaa atctgaattg acaataaatc ctcaactctg tcattcatat
    23641 atttttgtga atcgcagact cctttattgt aaaactccgg tgccaattct tctataataa
    23701 agtccagtat caggcttgca gccaaatcgc ccagttcttc atccctttct ttcagaaaat
    23761 aatactttat tgcagaaagc atatcatctc ttttttcttt ggatacttta attctatccc
    23821 tattcttcat gtaatgcctc cttgattctt agttatattt atattttatc agtacatatg
    23881 ccttcctaca attatgaaaa ttgcaaaaaa taaaatacgg cagatgcagc gcctgccgta
    23941 ttttacttcc ttatgcattt atcctaagct tttttatttc tggtctttac gtcaaaaatt
    24001 actgccgcta ccaataccgc gcctctgatt atgtactgat aggaaatatc aacacccatc
    24061 agattcatgc cattggtcag ggatgccata acaaaggcac cgataattga gcctgtgacc
    24121 ttgcctgcac cgccggctgc cgatgctccc cccacatatg cagctgcaat tgcatccagc
    24181 tcaaaggcga gccccgctgt agttgttgca gactgaaggc gggcagtata taatatcccg
    24241 gacaaagctg ccagcatact catagaactg aatacaatca tagatactct gtttacattt
    24301 ataccactca gctttgcggc atcgggattt ccccctattg catatatatg tctgcccaat
    24361 accgtttttt cggtaataaa attatatacc agcacaacta ttatgactac tactactgtc
    24421 caggaaaacc cgttataacc tgcaagtatc catgttatgt atccgattac cagggataca
    24481 aacaacagtt tgacggtaaa catcacattg gataaaactt caaagccata tttcagttta
    24541 ttattgcgat tgttgaattc cgccaaaata taaagtacaa tcgctgccgc acccaaaaca
    24601 agtgttaatc cgtgaacacc gtttatgctg aataatttcg gtatataccc gtttccgata
    24661 gcattaaaat cggcatttgg aactattatc gttcccgtgg atgaggtaac cctgagcaaa
    24721 gcccccctga atacctgcat acctgccaaa gtcacaacaa aggaaggtat actcatttta
    24781 gtaaccaaaa agccgattat gattcctatt gatgccccca aaaccaatac aactgcaatt
    24841 gtgatccaga caggcattgc atattttgtc aataatattg cggcaatagc tcccagaaaa
    24901 ccggcaatat atcctacgga taaatctata tgcctgatta ctattataag ggtcatgcct
    24961 acggccagaa ccgcaatata cccggtctga ttaatcaggt tgctgatatt ccttgaggac
    25021 ataaaaagac cgtctgttgc cattgaaaaa gtcagcatga tgacaaccag agctatatac
    25081 atcccatatt cacgtatatt cttctttatt aaatctctcg cttcattaac aattcccata
    25141 accaagcctc ctagttagtt gccatttcca taattttttc ttgagtcgct ccttctgcgg
    25201 gcaattcacc tgtgattctc ccgtcagata caacatatat cctgtcactc atacccataa
    25261 tctccggcaa ttcagatgaa atcattatta tactcatacc gtcttctacc aatcggttca
    25321 ttaaggtata tatctcatac tttgcgccta catcgattcc tcttgtaggc tcgtccagaa
    25381 tcagtacctt tggcttaacg tacagccact tcccgagaga aaccttctgc tggttgccgc
    25441 cgctcaggtt ctcaagtttt tgttctatac tgggagcttt tatatctaat gaacccttgt
    25501 aataattggc ggtttttact tcctcatttt catttacaat gccttgcttt gccagggctt
    25561 tcagattggc aatggtaatg tttgtcttaa tgtcgcttat cagtatcagt ccgttgccct
    25621 ttctgtcttc cgtaacatat gcgacaccgg cttttattgc ttcatggggg cctctgaagt
    25681 gtttcttttg ccccataaca tacaagtcac cgctgatttt atattcgtcc ggatttccaa
    25741 aaatgctgtg ggcaagctcg gtacgtcccg ctcccattag gccggctata ccgacaattt
    25801 cacctttttt cacaaaaaaa ctcatatcct taaggatttc tctccctgtc tttgaatcac
    25861 aggcattcca gttattaact tccagagtct tctcctcata tttttgcacg tttcttttag
    25921 gatagatatt ttcaatttca cggcctacca tatgctttat tatatttcct tcaagaatcc
    25981 ctccatcccg ggctgacatt gaagctacgg tccgcccgtc ccgaagcacc gtaacattat
    26041 ctgctatttc aataacttcc ttgattttat gcgatatcat tattgaagtt actccgtgct
    26101 ccttaagctc tcttaaaagc tccagcaggt tttcgctgtc atcctcatta agtgcggcag
    26161 taggttcatc caatatcagc agctttacat tcttgctcaa ggcttttgct atctcaacca
    26221 actgcctctt gccaaccccc aggtccttaa ccttggttgc aggattcaga ttgttcagcc
    26281 tgaccttctt taaggcctcc cctgcctgca agatggtttc gttccagtca atcaatcttc
    26341 ctttttttat ttcatggcct aaatatatgt tctcgtatac ggtcatttcg ggtactaaag
    26401 ccaattcttg atatataatt gcaatcccgg ctttttcact gtcactgatc cctgcaaatt
    26461 ttctgacact gccgtctagt ataatgtctc cggagtattc tccaaagggg taaacgccgc
    26521 ttagaacctt cattaaggtt gatttccccg ctccgttttc tcctaccagg cagtggattt
    26581 cgccattttc cacctgaaaa tttacattgt ccagagcctt tactccagga aagacctttg
    26641 tgatattatt cattttcaat atatattcct tcatagccat atgctctcct cgataaatca
    26701 atcacagggt tacaaaaggt atgccaatat atagctgaat agtgatagac agagctatca
    26761 ctattcagct atttttattt atgcaaataa ttacttcagg ttggtaaaat ctgatgcagg
    26821 atagtatcct gagtcaatca atgctgattt aacattatca gctttgacaa cgataacttc
    26881 ggtttgcttt gcttttacgt ctatttttcc gttattgtat gagcctgtcg tttgcggctc
    26941 ttttccttcc aataatgaaa ctgccattga tattgagtct ttaacaaggg tacggacatc
    27001 tttgaaaaca gtcattgact gtttaccgtc aataatgtat tgaacagaag ctttttcagc
    27061 atcctggcct gttaccttat agctggttac tgccgggtcg gcggcaaatg catcagctat
    27121 tgcacgggca gtaccatcat ttggcgctaa tatgaacaca ttgcccttat cagctgcctg
    27181 tgctgctgtc aggtgtgctt cagctttgtt cttggcttca ttgaaatccc agtttgtagt
    27241 tacctggcca attatcttac ccatttcgtc tctggtgagc ttggctttcc cctgaagggc
    27301 aactgcttcg cttgagttct ttattacaaa tgttccgtcg gctattttgg gctgcaatac
    27361 attccaggcc ccttcaaaga ataagaaagc attattatct gaagctgcgc ctgcatacaa
    27421 gtataacgga ttaccgctgc ctgatgcctg atcgaccagg tattttgcct gagcttctcc
    27481 gacagctatg ctgtcaaagg taacataata atcaaccgca tctgttccgg ttatcagcct
    27541 gtcataagat ataaccttga tgccttcttt tctggccgct tctactgcag atgctgcagc
    27601 atcgccatca tgaggacaaa ttattagaac atttattcct tttgcaatca aagcatcaac
    27661 attctctttt tcctttgccg aagaaccctg gctgaacaga atttccactg aataatcagt
    27721 acttcccaag gcttcgctaa accttgtttc gtcttgaatc catcttggtt catcctttgt
    27781 cggaagcact ataccgacat ctaccgtttt ggaatctccc ccggtctcac cggatgcagg
    27841 agccgagccg cacgccgtca atgcaaacaa cattgcaaat gccattaata ctgcaataag
    27901 ccttttcatt ttttcgtccc ccttaataat atttttgata tcaagttttg aaagctgcat
    27961 tctgcagctt ccggttcaat tatttcatga attatacatc atgtctctgt atgcatttgc
    28021 cggattacat tactatttgg cgcaatttaa aaacacccca atatgacagt aatattttgc
    28081 tgtaaaataa taccgcctgc aagctaaatc ctgcaggcgg catatttaat gcctataaac
    28141 ctcatcttca aggtgaaatc catctctgat tatcgtctca tctatatttt ctttatcaac
    28201 tgctattggg gtcaggacat aatattttac ctcataggta ccgtcattta tatagtcctg
    28261 tacattcagc ggctccttct ttacaagtct gactgccatt tcaaccgctg cctcggccag
    28321 cttttctatt ggcttgtata cggtcatcag ctgcatacct tcaactatcc tctgacatgc
    28381 cgtcaagtcc gcatcctgtc ctgtaacgga tacctttccg gccagcctat gctcggaaag
    28441 cgcttctatt accccctctg caaggctgtc atttcccgaa attacggcat ctattcttat
    28501 ccctttctgc agcatatcgt ctacaacctt gaatgcgtat tccttcagcc aatctgcagc
    28561 ccattcctct gcaataatct taatttttcc ctccgcttcc aacggcccca agatcttatc
    28621 ataaccctgt tttatcatct ttgtattgtt atcggttttg gcgccgttta ctatcagcag
    28681 gttttcccct ttcatgctgt ctgtaatcct ctctgccatc agctctccaa ccttgacatt
    28741 gtcaaatgtg acatacaaat ccacatttga gttcaatacc agcctgtcat aggatataac
    28801 aggtatcccc tgccttttgg ccagttccac tgcatgggca gctttattca ggtcgttggg
    28861 aacaattatc agtacatcta tgttttgctc aagcaaatat ctaacctgct tcatctggtc
    28921 ctggtcatca ttgtttgcat tttgtacaat aatatccgct ccaaattccc gtgcttttgc
    28981 tatcaaaata tccctatcct ttatccacct ctcttccttt aaagttccga gagaaaagcc
    29041 gatttttatc ttttctcttt cttcaatatt tttattgctt tgaaaaaagc ctctgtctgt
    29101 tctgttcaat atactggcaa ttgagagtat aagcataagg aatacagtaa aaagcaccac
    29161 aatgattgag ttttttactt tattttcctt agccaatatc atcacctctt atgtttatta
    29221 ctgctctata ttctgtgggt gtcattcccg tgatcttctt gaatattttg cagtagtaat
    29281 tgggatcgtt ataccctatc ttgaagcaca cttcttttat gctgattttg tgatccttca
    29341 ggaatcccat tgatttttct attctcaact cggtgagata gtcggaaaag ttcttgcccg
    29401 cttcatcctt gaatattttg ctgaaataat gataactgag atttacatat cttgctgcat
    29461 cggcaaggtt tatttccttg tggtaattat tatttagata ttccagcacc ttcggaatta
    29521 ttccatccct ttcgcttctt atttgctcct gtatctcctc cgacagctgg gagatgatat
    29581 ccataaactg tcttttcaat acttccttgc tgttggtttt taatatgctg aaaatataag
    29641 attgttttga tttgctgaag gagcttgcct tatacggcag ggctttttct atgacaaata
    29701 gcaggtcaat aagcttggac tttattttat ccatgtcctc ataatagttg gtgattaacc
    29761 aaagatatat ttcttcaaag gcttccccgg caccttgcag gttccccgta agcatcctgt
    29821 ttgcaaatat tgcctccttt tgtgcgggat aggtatccgg tatattgttg gatggcgtaa
    29881 tgtcctcgta atgcataaca gcatgtccat ttgatacaga ggcagctata taagcctcat
    29941 tgcacgactt gttaaagctt cctgcctcat agcttcttcc tatacctatt gtgtattcga
    30001 tacccgtacc gggtttgatt ctgtcaataa tccgatttgc aatttttata gattcgtttc
    30061 tgatttcata agagtcaatc cccttttccg aaggtataaa tgccgtgatt ctatccaata
    30121 acggatttcc tataagacaa gggcatatcc gctttaactc aagagaaaat ttctcataga
    30181 aattctgttt ctccaagctt aatttcaagt tgtcttcttt tatcttcccc tcaaaatttt
    30241 tcaaaagaac tgttaatgca taaccctgcc tcagatttat attgaaaatt tcctcataaa
    30301 attcaatatc gcttattgtt cctgaagtaa acagctgata ggatacaaac tgactttcaa
    30361 taaagggtat aatcctgttg atcctttcct tcaattcagc ttccttcagc aaaagcctcc
    30421 ggttttgttt gatggtattg cataagttgt ttagtgtatc aatcactttg ctcttttcta
    30481 taggcttcag aatatattca gatactccca gattaattgc ctccttggca taatcaaaga
    30541 attcataagc cgtaataatt acaaacagaa tactgctgtt aacggctttt atctgtctga
    30601 ttgcctcaat gccgtcaata ccgggcatgt gaatatccat aaatataata tccggcttca
    30661 gctcaatagc cttttcaatg gcttcccttc ccgttgaagc tgttgccacg atatctatgt
    30721 ccttcatatt cttccggata atcattttta aggagtccag aacaaggtat tcatcatcag
    30781 ctatcagaag ctttgtcata tgtatcctca ccctcccttc ttgcaccttt cattatcgga
    30841 agcttcaagg taacctttgt gccgtggcct atttcactct caatcgataa cagcttcctt
    30901 ggatcttcta tgttaaaaaa taattgcagc ctgtatagca cattgctcat tccaatgccc
    30961 gtaagacgat tcctgtactt actgccatcc tcttcaaacc gaagtatttc ttcaatgcag
    31021 gcagaatcca ttccaattcc gttatcaata acctctacca ttatgcagtc cccctcccgc
    31081 cttacattta aatgaatttc tccatgcctc tcaaggttct ggaggccatg gataaaagca
    31141 ttctcaacaa taggctggat tactattctg ggtatcataa tgtcaaaggt cgtttcatca
    31201 atatcggtgt aaaagtctat tctgctgccg aaccttgtct taagaatttg catatagtat
    31261 ttcacatact gaatttcatc ctccagggtt gtcagctcat ctatgttctt taaattgtac
    31321 ctgaataagt tggatatgct tacaatgaat tcggaggact tgtccgcgcc ttctatggca
    31381 gccaattgag cagctgcatt cagagtgttg aaaagaaaat ggggattaat ctgggattga
    31441 agaaatttca attccgcctc cttaagatga ctcatcatct tcaggttctg catttcctgc
    31501 tccttaagac gcttctccac ctctgcctgt ctctttaatt cattgatgta tgctttgata
    31561 ctgtttacca tttttataaa ggctcccgcc agtatatccg tttcatcccc ggtatgagtc
    31621 tgcctcagct caatgtcaaa atccccttcg gcaatttttt ctgcggaata ggacagctct
    31681 gaaataggct tggtcaaccg ataggttgac acaaccgcaa tataaatgtt tgccagcagc
    31741 gcggcaataa ttatcaataa gttcatataa cttataaaat tcatggtctt attgatattt
    31801 tcatatttct ccgttcctga ttttaattta ctgtccagaa tcttattatt ataaagctta
    31861 atatagtcgc ttatacgatt tgatctttga aaatgtgaaa tatatctgct gctgatcctt
    31921 ccgcgcttcg cctgtatggc attgtccgtc tctataagca gctcatctat catattcccg
    31981 atatccttca tgagcagcaa ttcattatta tactctgcag ttctgggtat ctttcttgtg
    32041 gtttcctgca atatattgta atgagtatag taattaagca ggctttctga ggatgctgtc
    32101 atcagatact tttccagctc agtcataagc gagtttacat cgttgttcag gctgttcagg
    32161 taggaataat cagtaataat gctgtttgta ttcctcaaaa ccacccttgc attgtaatac
    32221 gaaaaaatgc ttataacacc cagaagcaat atggtcagca gaaaatacat tatcaatttt
    32281 tttcttatac caataaaggt taataatcca gggttcatat tctcaccgtc tattccaatt
    32341 ctccgcagcc cttttaatcc agtttgtatg cctttttgta gctgctcaga tttttctttg
    32401 cgataacctt aacctgtgca tcaataaaac tggggacact tttgccattc tttacgtcta
    32461 caagtgccct gacgctttca tatcccattt tgtacgggtc gctggcaaca gttccgtaga
    32521 ttatttcttt atcaatgtac ctcagtattt cttctgaatt gccatatccc accaatacta
    32581 ttttacctac tttgcttctg tcaacaatca actgggccga gcccaaggta tcggctgaac
    32641 cggcggtaaa aattgcattg attccctcat cgctgtcaat aatgctctga gttatctctt
    32701 ccgcgctcaa tatgcccatc tttgaggtat aggtccttat tattttcata ttgggatatt
    32761 ctttcagcgt gctgataaaa ccgttcattt tcaaattata ctctatagct tcctgctcca
    32821 aatcaccgtt taagattact gcaaccttcg catcggcatt ggtggcttcg attaataatc
    32881 tggccgcttc cctccccaat tcaaagctgt ttgtacccgc aaaggcatat cttttgctct
    32941 cactgtcatc attttctaca gttatgacag gaattccatt gtcgtaagct ttatcaatca
    33001 tttctgtaaa gcccgtgccc cctacatggg taattattcc gtcaaccttt gatataatcg
    33061 caatatccat aaactgcagt tcctcttcag gattactgaa acgaggtgcg ttgtattcga
    33121 ctactacatt attatcttca gctgaattct gaactccttg cattacttca ttccaaaagg
    33181 gatcgacaga attctgcgca ataaagtaga aatgatattc cggcttggaa atataggtct
    33241 ttatatcctc cctctgcagc cagaacagca cagcacttgc agctattacc atcagagtca
    33301 gtatcaaaat agcatgtaca tataagaatc gcagcagatt tctaagtatc ttcattagca
    33361 tatttcccct taaatggaat tatgtatctt atattataac acagaaaaaa tagtttttgc
    33421 tttgcgtgaa aaataaaaag ggccaggctt gccatgcagc tgtcctgccc ttatcctatc
    33481 attatgcgat tgaatccatg aagataacag tcagttctta tctatttgaa ttatgaaaaa
    33541 acaataccgg gtttcaccat ttttctgtaa ttcagccaca taaccggctg taatcttctc
    33601 attttcttcg taatgtgaac taaggcctgc ttgaagcagc ctctctgcag taaattcata
    33661 ttttgagtcc ccgatctttt ctacttcaac atccatatct aacggtgccc cgaaagaatc
    33721 gttataatta ggggctccct ccctgttaat ccagtatgcc ttaacgcttg cttttacatc
    33781 ttgctccttc atatccaggg ttattttcgt tcctctgggg acatatatca gcttttccct
    33841 gtcaatttcc aggtaagctt tcttaaaatc ctcctttgat gtaaagggcc ttgaagtaag
    33901 cgtatagttg atatcctgtt gaccaactct tatatgtatt tcattttgag ccgctttgtc
    33961 tgaggcttta tcgttactgt tatctgtgac gcaacccaat aatggtacga caataagcaa
    34021 aaccaatgca aaacttataa cccttttcat ttaaaactcc ttcccgcagg ccttgcagaa
    34081 taaacaaata tgttgatatc cacttccgtg gcaaattcac tcagcttctc cagggcgctt
    34141 gtttctgcat gccggctgta aggtgtcatc gtaaaaagat tcagaatatc ctgcttgctt
    34201 tttaatatga tttcgtagtt tacctttttc ttttccaaaa gggtaaaccc ttctcccaca
    34261 gttccctttt gaccgatttc ctccggactt tcatacagtg cttccctgat actgtataaa
    34321 tgccgtgccc cgggagatgc aactataagc tttccctttg gtttgagtat tcgcctgaac
    34381 tcgccttcat ccctgggtgc aaagctgcac agaatgcaat ccagtgtacc tttcaaaacc
    34441 ggaatgtgat aattgcttgc aactgcaaat ctgatgccct tgtccctgcc ggaggcataa
    34501 tgtattgcag cctttggaac atcaatgccg taaatatcag tttttataca gtcacagaaa
    34561 tctgtcaagc tgtttttcag cctgcagata taataccctt ccccgcatcc ggcatccaga
    34621 atgctgacgg tttcttccga atcttaatca attaacagcg cggatagaac aatgacattc
    34681 agcatattgg aaaatccctc ataatacccc ttatcaagga actccctgcg gctctgaagc
    34741 atctctttgc tgtctcccgg agtgcctgcc ccggtatggc tgggaagcag caggtttaca
    34801 tacccttttt ttgcaatttc aaagctgtgc ccggtggggc aaaaatattg tctttcccct
    34861 tgctttaatg ctttaaagca atttgggcat tgaaatatgg aatgcatcat tatccttgcc
    34921 atagatgccg ctccatatcg atagttccgt ctgccctgaa ggtaatgccc tcagcttcaa
    34981 gcattgccct ttgtatttcg gggtctccga acacatggcc tggggatagc tcccccgttg
    35041 ctttaaccac cctgtggcag ggaagtttca tgtcttcagg agcacttctc atagcccacc
    35101 ccactgtcct ggcacctccg gagcatccca gcatcctggc tattcctcca tatgcagcaa
    35161 ccttaccctc cggtattcgg ccaactattt cataaactct tttaaaaaaa ccttctctct
    35221 tgtcagacat taccttttct cctcttacac cgttatctcc aatctatttc cgtcaggatc
    35281 caatatgcag ctttcataat agccgtctcc agtatgacga ggctcgctga ctaccgtata
    35341 acccgctttc cttaattcat ccgtaagact gttaaccttt tcggcacttc ctgttgaaac
    35401 agcaatatga gttaacccga tatgctgatt gattgtatca tttgaatcca atggagaccc
    35461 ggacatctgc ataagttcaa gtcttgcacc tgaatcaaat ttaataaaat acgattcgaa
    35521 agacttcttg ggatttatat atttctctcc ggcaatccca tcaaaataat ttgtataaaa
    35581 catttttaat ctttcaatat ctttggtcca tatagctata tgctcgattt tcatgattaa
    35641 tataatcccc ctccttaaat tcttacgaaa agctatataa aagacatatt agaagaataa
    35701 tgcgacgggg acacggggac gtttcgcgtg tcccactgaa agcgggacag gggaaacgtc
    35761 cccatgtccc cgggagaaac gtccccgtgt cctcgtgtcg cattcttcat attatgcgat
    35821 gcaattagtt atgccttggt tagactttat ctgattattt catacttttc tgcagacagt
    35881 gcatttatcg ccgtatcaat atttttatcc ttgacaagaa tataatctgt atcgtatgta
    35941 gaaattgcaa atatgcttat ccccttattt gcaagtgttg tactgattga ggaaaggatt
    36001 ccgatcaatg aaaaatccaa tggaccttca acccttaata ttctccaatc cttttcacac
    36061 tttattttat ccggaatatt atcttgagaa catacaactg ataattcatc cagcgttttt
    36121 gtaacggaat aaaagctgca ttgggatacc cattcgggaa ttgcctcgtc cttgtctaac
    36181 ctgcagactc catacaatcc ctccattaac ttcattgtta aagctttttc tgtcatatct
    36241 cctcttcctc ctctgatatc ttagtcatat tattgtctct tttcgtacat aatcaacaat
    36301 tttttaccat tgcccgacgc atattatcct ttcacatact gcaactaaca acgttgtaac
    36361 gatattggac aatagaattg aaacaatcag attctttctg ttcaaagaat ctttcctgag
    36421 cagaaaatac acaattggca attctacggc aacagtcaat attaagtatc ctgccagtac
    36481 aatatagtag gggcctttat tgaaagctgc aaatatggaa tagccgccgc ttgttggaat
    36541 aaatctgtat gccctctcca aatatggtgc aataaaagac attaggtttg caaggcatat
    36601 aatataaaat gctttcctgg aatctgaaac tccgcctaat tttgttacga aagctgtttc
    36661 aataagtatt gtaaaaacaa ctgcaaaggg caagaccttc attgggcttg cagtcaccca
    36721 atgccaggat gaatttgcat agacaatcga cgaaagtaga aataagaagg caccagccca
    36781 tacggctgct ttataggctt tatgtttcat atatacccca ccctcactca aatttcttca
    36841 taatacataa atattatgca atatcgaact tataccgtta actccaatct gtttccatca
    36901 gggtccaata tacagtttcc atagcaaccg tctactgtat aacgaggctc actgactacc
    36961 gtataatctg cactcattaa ttcatacgta aggctgttaa ccttctcaat atctcctaat
    37021 gaaacagcaa tgtgtgttag tccgttatac cggcaggact gtaattatat atcctcccgc
    37081 aaacaagctc aggaaattcg ctgctattac atataatact gttctcctgc gactatgctc
    37141 ttttatcaat gtaaggaata aaacaatttc tgcaatgaat acaaagaatt cagcaaatat
    37201 cagtgaaaaa accagatagc ttgccaaagg gacagagcta ttcagccaaa tatttaatgc
    37261 tccctgagtg atcagattta ttattagaaa agcaagccat gagttctttt tcctgaaaac
    37321 gaacaaccag aatacaattg cttctattat cagtgtcagg ataacacgca gagacactaa
    37381 gagaattgac cttgacaacg atttgccggg tgtaagtgtt tgattctcca aatccaatgt
    37441 atagatgttg ttgtatgttt tcagaggttc ttccagtaat acttcaaaac tgtcctctcc
    37501 cgtactgata ttgatattgt aatcctttgc tatccttaaa tctgccgagt aaaaaacata
    37561 atacctttca ataattttat ccctcatgct tgctttaata tctgccgggc caatgcttat
    37621 ctccagttca tcaggcgcgt caggggctat aatcagtatt gacggcggct ctgcagaatt
    37681 tccatagcac acagcagaga cgatgattaa cattgccatt gacaaaatca ttatcaggag
    37741 cagtttgtct tttttacaca tctatgacag cctcctttta aagcattgaa agcattgggg
    37801 acggttcttt ttgcttccga aactcagctt cttttcttta aatcctttcc gaaatgcact
    37861 atacttgcac ccaatgcgga taataccgta taaacaaata cccatacaag aaaagaacta
    37921 ttgaatactg taaatgcagc aatagaccac aatgcaaaag ttattcccgc aactatgaat
    37981 atttttctta tcaatacttg cccgataata ccaaacaaaa aggataataa gggaaagata
    38041 agcaatgcca tcagaaattc catagttaac ccctcctata aacaaaatgt cattaataag
    38101 aatgatgcgt attaacatgt acgggtagct tagaatgact tattaattat tcttccttca
    38161 ataattttaa tttctttaat aaatagctcc atgctgatta tttgctcttt agtcagacct
    38221 tttgcatcaa agtggttatc caaatttcaa taaatagctc catctaagcc gttaatgatt
    38281 tcgataccgg aaaccaatcc gtcttcaatg agaaaaacca cgcttttggt gccttcttca
    38341 ggggtatggg cataggcaag gtcacatttg ccgaaccata cctcgtcgtc gtaggagatg
    38401 ctgtccatct tctttagcgg tttttcggtc caatgttcta aaagggtttc ctctgtgtcg
    38461 cctatggtga cgcccgctga ggaacagcct tccttgacag tacggatagt gataatggta
    38521 tgcacgcctt catcaggatt aagatacgtt acctgcagat tatcgctttc cacaacagtt
    38581 acgttaccaa agctctcagc cctgaagctc ttgcggctct tctcggtcag atccaagctg
    38641 aaagggaaat ccaccggata cgcattcaga gatagggtgc cggttccggg gatagaaaga
    38701 gaaaatatgc ttccgtctga gcgttggtct aagcccacat ccgccttgtt gccgtcttca
    38761 attggttttt cacaatacac atttgataca aagagatagc tgccgtcttt ttcctgcagt
    38821 gtcagtatct ttttcccgtc ggaaaaaaag acgtcatcgt agaaaagacg gacgatatcc
    38881 ccatcccgct cgcctccgga aaaaataatc tccattctat aattggtgtc accgtggtaa
    38941 tagtaatagg catcatattc ctccaggtaa gtaaaattct ccagccctat ttcatctgta
    39001 tccgctaaag tcagtcccat atatttgcca agcaccgact ccatgttgga gcggctgatt
    39061 ttcatacagg cgcaatccgg ctccatatcc cagttgttat atgcaacaac agctgctctt
    39121 tcctcttctg tggaaatttc ttcaatgccg ctgccgcaat agaataattg aaatatatcg
    39181 attttttcgg gtctttcata tgtggagctc aaaaactgat tgcggatatt caagtattct
    39241 ccgttaaaga attcgtctcc gttgaagtat gaaagctcct catcggtcaa agctgttatc
    39301 tccttatgac ttttcccggt atcattacat ccggatacaa aggccataat gagaaagatg
    39361 gcaagtattg tgctgagcag cacattaggt ttttttacaa tcatcataat ctactcctca
    39421 atttactcgg aaactttttg atatagctaa aattacctgc tgttccattc ctctaataat
    39481 agacgaataa agtttccaaa tgtttcatat atcaagaatg atgcgacggg gacatgggga
    39541 cgcggggaca tggggacgcg gggacatggg gacgtttccc ctgtcccgct ttcagtggga
    39601 cacgcgaaac gtccccgtgt ccccgcccgt gtccccgcgt ccccatagca tttttattct
    39661 catatgcaat ttcctctttc cattataacg caaatccaaa tataatttaa caataatcgg
    39721 ataatatatg cgaacatatg tttgcttttt gcggtaaagt ttggtaatat aataatataa
    39781 atatcttcag gggtgttttt atgaacctta atgagaaaat caaaatatta tcggattctg
    39841 cgaaatatga tgtatcctgc tcctccagcg gaagcagcag aaagaacaaa aaaggcggta
    39901 taggaaatgc tgctccttca ggcatctgcc acagctggac tcctgacggc aggtgtatat
    39961 cactgttgaa aatactgctt acaaactact gcatatacga ttgcaaatat tgtgtcaacc
    40021 gctcttccaa cgatgttccc agagctgcct ttaccatcga tgaactggtt aaccttacaa
    40081 taaattttta taggaggaat tatattgaag gccttttcct aagctccgcc gtatgcgtaa
    40141 gccccgacca taccatggag ctgctcctga aatctgtcaa aaggcttcgg gaggaggtca
    40201 atttcaacgg atacattcat gtaaaggcca ttccgggagc aagcaataag ctgatagagg
    40261 agacaggaaa atatgtggac aggatgagtg tcaacattga gcttccttcg ggcgaaagcc
    40321 tcaagctgct ggcgccccag aagaccaagg aatcaataat caagcctatg gggctcataa
    40381 agtcaggaat aattcaatat caggaagaaa agcttaagtt taaaagcact ccccaatttg
    40441 tcccgggagg ccaatccacc caattaatcg ttggtgccac aaaggataat gatttaaaga
    40501 ttttgaagct gtccgagtct ctttatagca gctacaagct aaaaagggta tactattccg
    40561 cctatgtgcc ggtatcaaat caccctaatc ttccggcaat cagcggtccg cccctattga
    40621 gggagcaccg gctgtatcag gcagactggc tcctccggtt ttatggtttt aactccggtg
    40681 agttattaaa tgaagaaaac ccgaactttg atgagctgtt ggatccaaaa agcggctggg
    40741 ctatcaggaa ccttgacaaa tttcccgtag aagtcaacaa agcagattat tatacattgc
    40801 tgagagtacc cggaatagga gtcaaatcag ctcaacggat agttcagggg agaagggtat
    40861 ccatgcttga ctttgatgac ctaaaaaaac tcggaatagt tctaaaacgg gcaaaatact
    40921 tcattacctg caaaggcagg tatttcccgc aaaaaagcat accgaccaat gctgtatcca
    40981 taaaaaacag gctgctgctg gaggataata ttaagaaccg cggaaatatt gagcagctct
    41041 ccatattttc acttttcccg ggcataacgg atggtgaatt ttgatgctgt attatatcta
    41101 tgacggcagc tttgacgggc ttttgacagc catatatgat gcatactaca ataaagattt
    41161 tcctgagcaa atagtacccc aggacagctt cacagacaac tttttgatta acaaggttta
    41221 catccggacg gaagaagaga aatcaaggaa agtccatgat gccgtacttc aaaaaatatc
    41281 aagacgggca atggctggta ttatgtatgc ttatttatcg gaagatgaga atgccggaat
    41341 atctattttg gaatacctgc gttttggctt caaagtcgga agaactgtag attcttatct
    41401 cgccgatgac agggtaaaca atattaacag actggtaaca aaggttgcgc gggaaaagca
    41461 tctgctggca ggaattataa ggtttcgtct gcttcataac aacatttatt atgctcccat
    41521 tgagcctcag tataatacat tatgcctcct gggtaaacat tttgccgaca ggatgtctga
    41581 tcagatattt gtcatacatg atgtgaaaag aagctatgga gttttttacg acaagacgaa
    41641 atggttcatg agtgatattg aaatgaataa ggacattgta ttgcacgaaa atgaactatt
    41701 ctaccaggag ctttggaaac gatattttga taatataacc ataagaagca ggataaatcc
    41761 aaagctccag tcaaatcaca tgcctaaaaa gtattggaag tatttaattg aaatgaaata
    41821 atcatcttgt agtcaatgtg ctgttgtttt tgcccataat cacttttgta tttactttac
    41881 cgatcctgac tttttcgatc catgccgaca gagtatccag cgccgcttca aggtctttgt
    41941 gccttaaggg gtccaccgac tcaataacag cgattgcatt tttcgtatct tcggtaagca
    42001 tcgttctctg tccgtccctc cagttccagc agcggcatac cgctccttca tcgtccagat
    42061 agcagatttc tccggggaga gtgtaatcaa tttcttcatc gcctaaagca agaaacgggt
    42121 cacctccatt tgtaactgta agtctaagat ctcctttgaa tgtatcaaca tcctccattc
    42181 cgcacggtaa ggcgaatcgg agcgaaattc cattatatat gtctaccagc gggttaatca
    42241 ctcccacttc atttcctttt tcgatgcgtt taagcagggc ttcaatggag gaccttgccc
    42301 ctttttttgt cttaaatttt gaaaatgcat ccctccaaac ggatattact ctgttttggc
    42361 tcagtacagg ttccgttaaa tacttcctcg cttcctggtt tgattgttca aggaattcaa
    42421 taatatcaga ccggagcttt tcgcatccct cctcggtgtt gtcaatatca ttaatcaaca
    42481 gtacaccaat ctcacattca gggaagatat cccagaaagg ctgttcaatt ataaatctgc
    42541 tcattttctt cctccgttct tttttcgtca tattttaaga ataatgcgtc gcaggtaacg
    42601 ggtaacacta aacacacgga tgtatcacgg attcctgcgg aataacacgg taactattat
    42661 atattatata gctgcatcag gaaactttga tacttacatc attatcgata aatatttatg
    42721 tgccgaaata tttatcccgc cgtgcattta gtgatttttc gtgtcttcag tgttaccaag
    42781 gcttcaaagc cctaccctga atctcgtatc tggtgtctcg cgtctagtgt ctgtgcacgt
    42841 tagtgcgcat cattctctgc tttttttctt tttaagcaat gtggatgtat ccagattttc
    42901 tgtcttaggt ttttccggtt ttgcttcctt ctcctttgcc tccggctttc cctcagcctg
    42961 cggaggcata tgaaccttct tttcagcggc cttgagtgct tcatccatag tgctgatcgg
    43021 ccttttcacc tttgcagacc ttttaatctg tggaatcagc cttgcccttg cggccgaaag
    43081 cttttcctcc actgctgcca ggggcaggtt cagccttctt aaggcaatat caagcatata
    43141 caatataagc gcaagtataa gaagcgccgg ggtcaaatct accgtcccga aaatatcctt
    43201 tatttctcct ttgtatacat cttcaggagt attgataagc gtgcctcccg cttccttaat
    43261 cagtctgtcc aaatagcccc tctcaggctg tattctgtat tcgggagagt attgcaccga
    43321 tatccctgta ctggcggcat ttatcgtctc cccatctttc tgctgcaggg cttttatgat
    43381 atatgagcct gtctctgtta tttcaaagtc ccccctgtac tgccccggtg caacaggata
    43441 gagagtaatc tcactgctct ccagagaagg agacactatt gaggctttag actcaaactc
    43501 acccttatcc gccttgttgt taagagttat agtgcccttc cctccctgca cttcagcctc
    43561 tacagttata tcgtcctccc ggtatttttc aatagtccag ttgataagat tctgccacag
    43621 ctttatgtta ttctcccagc ctacataatt cctgctccat cttccgttta tatcggagtt
    43681 ccatgcagca gtcttaccca ggccatactg ccatactgta agtatcggat cctcctggtc
    43741 gcttgccagc accacccggg aggcactctt ggggctggat gccacatagc ccagaagtgc
    43801 cggcaatcct tcctcggctg ctccttccag caccggatga gtattggtta tagcaggtac
    43861 aaattccctg ttattaagat acgcccttgc agccataaag gtctcttttg cgaatatcct
    43921 cggaatgttt gcaaactcat cagtataata gaagcgtccg ttggctccct ttgctatatc
    43981 ctgaaggagc tggacatcag agccctgccc taccgctacg gttgataccg taatattgtc
    44041 cccggcaagc ttctcaacaa gttcagtgta tccggtcctc tcagcctggc cgtctgtgag
    44101 aagtattata tgctttattt ttgtatcggt tttctttatt gaatcatagc cttcctgcaa
    44161 ggcagggatt atgcttgttc ctcctcccgg ccttatggta cctatgtcat cccttatttt
    44221 ttccgcatca tccgccttgc gggtgggaac cacccagtag ttggtatcgt caaaggtcag
    44281 cacccctatc tgatccttgg gtcttaagga ctccagggtc cttgccgcag cttccttggc
    44341 aagatcaagc ttcattattc ccccccggcc ttctgtcatg cttccggatt tgtctattac
    44401 aaggacaatg gacatatccg gtatttcctt cctgcccttt agctccatat gtaccggaag
    44461 caccttttcc aggggtgttt tgtaataacc tcccaaagcg aaggaatttt ccccgccggc
    44521 agcaataaag cctcctccca tatctttgac ataggaatcc aaagaattca gaaagccgtc
    44581 gcttaggttt tccgcggata cattgcaggt aattattgac ttgtatgcag acaagccctc
    44641 caaggttccc ggtacaccgg cagcattaac tcttacgtag ttcatgccgg tagcttcaag
    44701 catcctggcc acctcatccg cctctcctgc cacgtcctca acaatcaaaa ttctgggcct
    44761 gtcaagtaca tttgtaaagg ttgaagcctc attattcctg ctttccgtgt cggtatccgg
    44821 ctccagaagc gccttatagc tcttgaagcc tcctgtttcc gccgtatccc ggaatacaaa
    44881 cctgttgctt cccttctgca gcgctacctt ctcttcggca gccttatcct ttccgctgat
    44941 aagtgtaagt tttgcctctg tctgaacagt gctttccaca gtcactataa tgttaaattc
    45001 ttctccgact ctcaggcttt tggggacagt tacactctct actgcagcct ctttgccgat
    45061 ttcacggttt atcctgtata ccttgaaatc aatatcctgc cctatcaggc tggaagccag
    45121 ctttgagctg ttaccctcat tctcctcccc atcggttatg agtactattc tttttttgct
    45181 gtcctggggc agtaatgata ctgatgtagt caatgcccct tctatatctg tataaattcc
    45241 tcttggctca gtttcaattt tattaaaaac cgaatccccg gatacaaagc tctcaattag
    45301 agaattatcc ccaaaagcca caacccctgt ttggtccttg gagcccttct tttccagggc
    45361 tttccttaca aaatcctcgg aggttgtcct gtattccctc atgctgtctg aggcgtcaat
    45421 cacaaatatt gtggtggttg tatccacaat ccacttaata ttcacaccgg ttagtgcaag
    45481 tatcagaagc acaaatacac tgttcctgag aaacaatatc agccttcttc tgagcctata
    45541 catttttctc agcttacggg cagtgaaaaa tacaaaaaat attgccaatg gaataagaaa
    45601 aagcataaga ggtctttcaa aatcaataac cacgtatata caccacccat tctatagctg
    45661 ccgccatcag catcaagccc aaaaacaact cttgaagtct tgtgcctccc gaagtattgc
    45721 cggcttcaga ggcttcttga gaaatgtcga cttcctgttt gctgtttacc gactcgcttt
    45781 ctgtgggaaa gttgacggca aagcttga
//
LOCUS       DAOQUR010000018        39550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1183_length_39550_cov_10.076060, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000018 DAOQUR010000000
VERSION     DAOQUR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 39550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39550
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1183_length_39550_cov_10.076060"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1047)
                     /locus_tag="PKI50_05685"
     CDS             complement(205..1047)
                     /locus_tag="PKI50_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNT03237.1"
                     /translation="MLNYKIVGNAMQQVVVELGADQTMYSEAGKFLWKTDNIKFETSF
                     NVGDKKGHFEEKSGGGVMGKFMDTAVQVGKRKLAGESAAFQVFNVTHGESGLVTFSQT
                     IPGEIKAINIEDFGVLYVQKDGFLAAENTVDFDIALTKKIGAGFFGGEGFILEKFSDV
                     GTLFIGACGNFIEINPADYGGKVQVDTGALVAFDKNIDYDIEWVGGSVGQVAKNLLFG
                     GEGLFLATLSGNGKVLIQSMNITSLARTLFRNATKSSPEDRSSGKMLGGLGSLLGELG
                     GDRY"
     gene            1364..3022
                     /locus_tag="PKI50_05690"
     CDS             1364..3022
                     /locus_tag="PKI50_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNT03238.1"
                     /translation="MQEMITLTFPDNTKCEYPKGMKLLEIAKDFKQHFNSDIVGAIVN
                     NELKELWVELRQDCSLQFIDRTTVYGNRFYSRSLAFLFIIAAKEVFKDCRVTVEHSLS
                     KGLYCEVHKDKPLAGEDIARIEEKMREFRNMDIPFVKRKMTSTEAIEIFTRTQQFDKV
                     NLIRYRKKKHINIYSCHGHYDYFYGYMVPSTGYLKIFELKANGPGLILRYPERSEPNK
                     LPEFKEQKKLFSIFREFEEWGKILELENISHLNDAVMDGRINDVIRVAEGLHEKKIAQ
                     IADMIVASGNKKLVLIAGPSSSGKTTFAERLSVQLRVNGLKPVAISIDDFFKNRADTP
                     IGEDGEYDFETIDAIDIATFNDVIARLINSEEVEMPVFNFHTGNREWKGRKLKIGKEQ
                     ILVVEGIHGLNEMLTQCIPKEQKFKIYISALTHLSIDDHNRIPTSDLRLIRRIVRDYQ
                     FRSADALSTIKRWDSVRRGEEKYIYPFQEEADVMFNSSLVYELAMLNQIVLPLLEKID
                     NGVHEYIEAKRIKKFLQYFLEADMNAIPSNSILREFIGDSCFFK"
     gene            3208..3888
                     /locus_tag="PKI50_05695"
     CDS             3208..3888
                     /locus_tag="PKI50_05695"
                     /inference="COORDINATES: protein motif:HMM:NF021120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HNT03239.1"
                     /translation="MRRKNIVISALVLCFALMFSACAARRPLQTPAQPGQDTAGRTGT
                     YTGIGPSTTNPYDTGYGEFPSYYGGYGGYGGYNNGMYGGYGNDYRNMGGSNYSPNVGM
                     NNTRLNTTQAQRLARACEAVPGVDNATCVISGNTCYIGVDTDGDLTGRNIAYGNANDL
                     SAVKRQCAQKVKAANPQIQTVYVSTDTNFFERLRRISDGAGNGNLGDNFRNELNELVR
                     GLTPERLQ"
     gene            4126..4491
                     /locus_tag="PKI50_05700"
     CDS             4126..4491
                     /locus_tag="PKI50_05700"
                     /inference="COORDINATES: protein motif:HMM:NF022158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HNT03240.1"
                     /translation="MGFTNSFNDFLFSVVPFFMFIIFVIVTMTFITSFTEGLGQYLRN
                     KSAPKKTIPARLAAKRTHDWGGYGDTDIRISYYAAFETENGERLEFPVSSSFSEMYAE
                     GDTGMLTYKGTKFIDFKIE"
     gene            4535..5452
                     /locus_tag="PKI50_05705"
     CDS             4535..5452
                     /locus_tag="PKI50_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNT03241.1"
                     /translation="MKAKRLKTGDTIGIVAPASPSETLKAERAIKHITDMGYSVKAGK
                     SVYSSRGYLAGADELRASDINDMFADDEISAVFCLRGGYGSQRILDLVDFERISSNPK
                     IFMGYSDITALLNAIYQKCGLITFHGPMGGDFAGGLEKRTKRAMKNILESTEPAGELP
                     NPVTPGVVEEGKASGKLAGGNLSIVAASLGTPYEVDTRNRILLLEDVFEEPYSVDRML
                     TQLKLAGKLKSAAGIILGDWGNSEPEEPEKSLSLEEVFEDIFKGLGKPVLKGLKIGHC
                     KPNFTVPIGAEVSIDTCSKTVCILESAVE"
     gene            5598..6884
                     /gene="tig"
                     /locus_tag="PKI50_05710"
     CDS             5598..6884
                     /gene="tig"
                     /locus_tag="PKI50_05710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNT03242.1"
                     /translation="MNSKLEKLENNVATLEISVSAEKLEEGIMKSYLKNVKKFNIAGF
                     RKGKAPRKIIERHYGEAVFYEDAINIICPDAYDEAVKEHNLEPVDRPDIDIVDIESGK
                     GLVFKAAVTVKPEVALGQYKGIEAEKKEYNVTDEEVDKEIETIREKNARLIDISDRPV
                     KNGDITIIDFKGFVEDKQFAGGTSENYSLEVGSGQFIPGFEEQLVGAELGKEIDVNVQ
                     FPKEYNSEELAGKPALFKVTVKEIKEKQLAELDDEFAKDVSEFDTLDELKEDIRKKKE
                     EQYKRLEKQQYEDEVINKVVENATVDIPEVMVDAQVRIMLRDFDYQLRYQGLNLEAYL
                     KYMNMDIEKLKESYRDTAKSRVKSQLVLEKVAEVEGIAAAEEDLNAEIEKTAKHYNQD
                     LEKFKKTLKEDEITYIKDGIVIQKAIDFLVDNSVAI"
     gene            6912..7496
                     /gene="clpP"
                     /locus_tag="PKI50_05715"
     CDS             6912..7496
                     /gene="clpP"
                     /locus_tag="PKI50_05715"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNT03243.1"
                     /translation="MSLVPIVVEQTNRGERSYDIYSRLLKDRIVFLGDEVNDVTASLV
                     VAQLLFLEADDPDKDINFYINSPGGSITAGFAIYDTMQYIKPDVSTICVGLAASMGAF
                     LLAAGAKGKRFCLPNSEVMIHQPSGGAQGKATDVGIYAERLIRSRKKLNEILSAKTGQ
                     PIEKIERDVERDYFMSAAEAKEYGLIDDILVKRK"
     gene            7511..8806
                     /gene="clpX"
                     /locus_tag="PKI50_05720"
     CDS             7511..8806
                     /gene="clpX"
                     /locus_tag="PKI50_05720"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HNT03244.1"
                     /translation="MAKFDDKKQLKCSFCGKNQEQVRRLIAGPGVYICDECIELCQEI
                     IDEEFYEEFDTELQDVPKPAQIKEFLDQYVIGQEEAKKALAVAVYNHYKRINSDYKID
                     DVELQKSNIALIGPTGSGKTLLAQTLAKMLNVPFAIADATSLTEAGYVGEDVENILLK
                     LIQNADYDIERAQKGIVYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVASV
                     PPQGGRKHPHQEFIQIDTTNILFICGGAFDGLEKIIMNRTGKKSMGFVADIHSRNHQD
                     IGEILKKIMPEDLLKFGLIPEFVGRIPIIVTLNPLDKDALVKILTEPKNALVKQYQKL
                     FELDNVDLEFEDDAIEEIAQKALDRKTGARGLRAIFEENMLNVMFEIPSRDDIDKCIV
                     KRETILEHKEPDIVYLEPGTKKTVKKQPRKGQKARKGTA"
     gene            8950..10665
                     /gene="lonB"
                     /locus_tag="PKI50_05725"
     CDS             8950..10665
                     /gene="lonB"
                     /locus_tag="PKI50_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HNT03245.1"
                     /translation="MSQLLIAIQLFFAVVIGMYFLNLLRGQQGNKVAIEKESRKEIEK
                     LQHMRDIQLTKPLSEITRPSDFREIIGQENGIKALRAAICSPNPQHVIIYGPPGIGKT
                     AAARLILEEAKKTPNCPFRQDAKFVEVDATTVRFDERGIADPLIGSVHDPIYQGAGAM
                     GVAGIPQPKVGAVTKAHGGILFIDEIGELHPIQMNKLLKVLEDRKVFLESAYYSSEDA
                     NIPFHIHDIFQNGLPADFRLIGATTRGPESIPPALRSRCLEIYFRALTSEEIGIVTSN
                     ALNKIRFECEEGVPELIGKYATNGREAVNIIQTAVGVNINEKRNKVTISDIEWVLNNG
                     QYSPRPERKINDEPQIGYVNGLAVYGPNMGTLMEIEAIAVRAGRGTGRLLVTGIVEEE
                     EIGSRDGRTIKRKSMAKASTDNVITAIKKYLGIDPGEYDIHLNFPGGTPVDGPSAGIT
                     IAVAIYSAITNSYVDNRIAMTGEVSIRGKVKGVGGIIPKIEAAVRAGVKKVIIPKENW
                     QEIFSTYDIEVISVESIVEAVNIATMRQAEVKHLKAQSSEGNILTASKIAKAPSMQAM
                     SDIKQ"
     gene            10758..13100
                     /gene="lon"
                     /locus_tag="PKI50_05730"
     CDS             10758..13100
                     /gene="lon"
                     /locus_tag="PKI50_05730"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNT03246.1"
                     /translation="MQKRKEDIKRLPLLPLRGLSIFPYMVLHFDVGREKSIRALEEAM
                     INDQLIFLVTQRDAHTDLPTVKDFYEVGTISKIKQLLKLPGDTIRVLVEGINRAEIRD
                     IISEDAFFLVDVLEHQDVQLSGDLELEALRRSVMEVFDEYIKANPKVAPETLITVSDI
                     DDASRFADVIASNLTIKIEEKQEILSLFDVKERMEKIFEILSRELEILEIERKINSRV
                     RKQIDKSQKEYYLREQLKAIQRELGEKEGHGEEIEQYAEKLSKLEVPDEVKEKVEKEL
                     DRLSKMSQGSPEVGVTRTYLDWIFDLPWNTLTEDNLDIKNARKVLDEDHYGLKKVKER
                     VLEYMAIRQLSNNMKGPILCLVGPPGVGKTSIARSIARAMGRKFARMSLGGVRDEAEI
                     RGHRRTYIGAIPGRIIYSIKQAGSRNPLFLFDEIDKLASDFRGDPASALLEVLDSEQN
                     KEFRDHYLELPFDLSKVMFLTTANSTDTIPPALLDRMEVIYISGYTEEEKLHIAADYL
                     VPKQIKEHSLKDESVSISEAVIRAVINGYTREAGVRNLEREIANIIRKCATQIVENNK
                     KRVRVTPGNLKKYLGIQKYRHDLINQKNEIGVVTGLAWTPVGGDTLFIEVSVMPGTGK
                     LELTGHLGDVMKESARAGLSYIRSRCEEFSIDKEFTKNMDIHIHVPEGATPKDGPSAG
                     ITMTTALVSALAKIPVHRNVAMTGEVTLRGRVLPIGGLKEKVLAAKRAGIKKVILPYE
                     NEKDIEEIPDTVKKNMTFVLVSDMDEVLSHALIRDEKHDN"
     gene            13090..13671
                     /gene="yihA"
                     /locus_tag="PKI50_05735"
     CDS             13090..13671
                     /gene="yihA"
                     /locus_tag="PKI50_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNT03247.1"
                     /translation="MIIKKCEFIKSAVHQNQYPELDLPEIAFSGRSNVGKSSLINSLL
                     NRKKLVKTSSSPGKTRLINFFMINDEFVLVDLPGYGYAAVSKSQRQAWGKIIENYLRY
                     RENLKNVVLLVDIRHEPTTDDKLMYDFIKYYRKSVTVVATKLDKITRSSVNKNLNIIK
                     QKLDINQNDILIPYSSETHVGREELWEVLLTWK"
     gene            13697..14401
                     /locus_tag="PKI50_05740"
     CDS             13697..14401
                     /locus_tag="PKI50_05740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNT03248.1"
                     /translation="MEVYEAFIIILTFLLLNVPIFISLYEYIFKDMSLIAKFAVGVVY
                     WSAAFLIHELAPFIGVLILLSKVHRKAEVEIHERNINIWSATAGDIIFVAGATLVFKA
                     VINQINKLYVYILNIYFNMDAKPQEIVEEFAGGEMYYKVMLFILVVILAPFVEEYIFR
                     YYIYDKLLLKSMPHAAAAIISTLIFTLLHFNISGIPTFLGLGLYCTFIYERKGFYGAV
                     TAHLVSNLVTAAMLIV"
     gene            14569..14952
                     /locus_tag="PKI50_05745"
     CDS             14569..14952
                     /locus_tag="PKI50_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03249.1"
                     /translation="MEIEFHRVLLDALTEAIMRKSVVTVKNMENDSIIVVTATEDEII
                     ISLSRIPSMMNDVISAVSDCLGRCPDMIYDRASVLKDDIVIYYMVWLNNNEGGRSFVE
                     KEFAVPQNRITYLSQKALGYLQNMN"
     gene            15126..15773
                     /locus_tag="PKI50_05750"
     CDS             15126..15773
                     /locus_tag="PKI50_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNT03250.1"
                     /translation="MLKKLKLFFIKPTELFRDYIEKPVWALKLFVIALVIGLYTYGTK
                     ILGKDLFIEMMEEKAAAMPPEQAEALRASIAFMNTPMMNLISAVVAAISTVAIILLVA
                     LVYKAFIRALKGEIKYKQVLAVYTLAYMASIVGIAVKFAYMYFTGNLLYLDLSPTFKD
                     VLLNNLDPFVIWQSILMVFGISAVSGIPEKKSIIIVAVMWLVALAISFGSVMLAN"
     gene            complement(15744..17093)
                     /locus_tag="PKI50_05755"
     CDS             complement(15744..17093)
                     /locus_tag="PKI50_05755"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNT03251.1"
                     /translation="MKYDIEIPIPRKVQFILDKLHEHNYQAFIVGGCVRDSILGKVPE
                     DWDIATDALPGEVKELFQKTADTGIRHGTVTVIIERSLFEVTTYRIDGEYADFRKPKA
                     VTFTSSIEEDLSRRDFTINAIAYSPEAGIVDPFGGVADIRKGIIRTVGDPERRFSEDA
                     LRMLRAVRFSAQLGFSVDEKTLKSIEINSSLIGCISPQRIRDELTKILLSDNPLCFSL
                     LKDTKLIKYTLPEFEPCFITNQNNPYHSYNVAEHTLRSVAHVEKDKILRWAMLLHDIG
                     KPGTKTTDSKGIDHFYNHQQLSVKLSGTAMSRLRFDKKSMDKILLLVRCHDMHIKADA
                     KSVRKAMSRVGDDMFESLLKVIEADKKAQNPGLLKERTGKFIKIWELYKDIKEKGQCT
                     SLKSLAVNGDDLIALGIKPGKKIKELLNSLLEKVIEEPELNKKEILLSLISQHHRAE"
     gene            complement(17111..17737)
                     /locus_tag="PKI50_05760"
     CDS             complement(17111..17737)
                     /locus_tag="PKI50_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HNT03252.1"
                     /translation="MSVPSFKRLPMHIGVIPDGNRRWALGKGLEKQDGYCHGIKPGFE
                     LYKLCIELGIKELTLYGFTEDNTRRPSIQTQAFRKACVDAVMELTGKDAELLVIGNTS
                     SPMFPQELLPYTKRAKFGRGLIKINFLVNYGWNWDLNHPEHIASADISRVNLIIRWGG
                     RRRLSGFLPIQSVYADFYIVDDLWPDFKPEHLYKALEWYQYQDVTLGG"
     assembly_gap    17883..17982
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18095..19072
                     /locus_tag="PKI50_05765"
     CDS             18095..19072
                     /locus_tag="PKI50_05765"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HNT03253.1"
                     /translation="MKKFIRIFALVLLIMFAVSGCFIKGADLPEDMVGMPSEGYGNTL
                     GNITNGGVVAFQEGWVYYCNGNDDVNIYKMRINGSEKTKLNSDKSMFLNVQGDWVYYS
                     NIEETEKSSIYKVKTDGTKRTKLTNDSASFINVVDEWIYYSNASDNRRIYKIKTDGTE
                     KTLIGDNFSSYLNVHDGWIYYTNGDAKKRIYKVNIEGTENVIVSEDSAAYINVDGDWI
                     YYSNGSEGNKLYKIRTDGTENTELTKEEALFINVSEEWVYYSNGYNGNRIFRIKKDGT
                     DKAILNYESSKFINIADGWLYFIVDSASYRLFKMKSDGTKIQMVEKVER"
     gene            complement(19092..20147)
                     /locus_tag="PKI50_05770"
     CDS             complement(19092..20147)
                     /locus_tag="PKI50_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03254.1"
                     /translation="MKIENSNICLKSEHSSVTFKQKQENMQFWIGDNPETSMNSPPGI
                     IIDISKKAMELHQQISSTNSQCIDTDIEEELAGEDKQKLMLIEAMLTQLLGKKFRFKI
                     PKKIRINSDMPINRGVPANQGNGNPRAGWGLIYDSKSIHYESEKVSFSSTGTIRTSDG
                     REIKIDLQLNMSREFFSSEEIHIRAGDALRKDPLVINFDAPSAILSDNKFSFDIDCDG
                     SPDQISRLLAGSGFLSLDLNNDGKINDGSELFGPQSGDGFADLSQYDLDENGWIDEND
                     IIFDRLRIWTKDEYGNDTLFALGEKGIGAIYLGSAGTAFSLRGEENKENGVIQKTGIF
                     LHENGTAGSVQHVDLIV"
     gene            20302..21753
                     /locus_tag="PKI50_05775"
     CDS             20302..21753
                     /locus_tag="PKI50_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT03255.1"
                     /translation="MGEVIIPEDIEINIDKSSGSPIYIQLYEQLKKYIIEGIVTEDCK
                     LPTIRDFAEHLGVNSITIVNAYKLLEDNKLVYKKVGSGTYVMPLKAVINLESNFTYSL
                     DEIEFDMDMGADEDIINLSTASPDPKLFPIKDFRRMLDEVLERDGGRAFMYHENMGYK
                     PLRESVSKYLTEYGINAEATDIHVISGAQQGIDIISRVLLTNGDCVFVESPTYNGAVA
                     VFKSRGAKIVEIPLMADGPDMKELEKKLALIKPKLIYVMPNFQNPTGYTYSERKRKYL
                     LLLCKKYDVTVVEDDYMSDLSYLDVGKRPLKALDREGRVIYIKSFSKIFMPGLRVGYL
                     IIPEGLKENVMGIKHITDIYTSGLMQRVLDLYFRSGIWSKHLEYMKREYSLRYFEAVR
                     SARKYLRGASFTQPYGGLNLWVKLPEKVSSVELFERCRDRKVIFTPGTVFIKGEAGEM
                     HIRISFAAASLGKIAEGISIIGNVMEEIKDSAK"
     gene            22260..23237
                     /locus_tag="PKI50_05780"
     CDS             22260..23237
                     /locus_tag="PKI50_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT03256.1"
                     /translation="MMKFYIGIDVGSVSTNVVAVDDSFNVLSKQYIRTNGQPLESVKE
                     GLRNLKAELGSGSKVLGAGVTGSGRQLSGIMVGADVVKNEITAHATAAITYFPDARTI
                     FEIGGQDSKIIMLKNGMVVDFAMNTVCAAGTGSFLEHQAERLKVRIEEFGDLALRAER
                     NVRIAGRCTVFAESDMIAKQQYGFSKADIINGLCEALVRNYINNLGRGKKLEPPFVFQ
                     GGVAANKGIKTAFEKVIGHQVVVPGHYNVMGAIGAAMLAREYILRSGKTTGFRGFEAD
                     DFDFVPASFECSDCDNSCEVIKVEMDGKVAAMWGDRCGKWTNSVNNNPL"
     gene            23467..23814
                     /locus_tag="PKI50_05785"
     CDS             23467..23814
                     /locus_tag="PKI50_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03257.1"
                     /translation="MFTLRSNEAGLAASGYTYLKYNDGVYVGAEKTESGDVKAEVTIK
                     NAKIKDIKLIEMPPDYISNNPNIKDEISDVIYTIIKKQDVAAASDTGTASYVLSKVIK
                     AVRNALNESMLVQ"
     gene            complement(23955..24371)
                     /locus_tag="PKI50_05790"
     CDS             complement(23955..24371)
                     /locus_tag="PKI50_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNT03258.1"
                     /translation="MIDLIKFLKEKGYKVTPQRIAVYDILKNTKEHPSVDMLYSKLQP
                     VYPTMSLATVYKSLEVFKDLGLVQELNVGEDKFRYDANVNQHPHITCIACGKVEDVYD
                     EMLFDLADQVQKKTGYQLTNQQLYFFGYCPSCKRNH"
     gene            complement(24831..26039)
                     /locus_tag="PKI50_05795"
     CDS             complement(24831..26039)
                     /locus_tag="PKI50_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT03259.1"
                     /translation="MKKSAYKRIALAMLTLIILFITVIPSYADTSVYKRGDSSAEIAE
                     IQAALKKLGFFDEVCTGYFGEVTEGAVLKFQEKYNLGTDGIIGPITIGKLNELTGGHI
                     AIPSRSSSAKKDTTKVNVRYKIKKGDTIWDIAGDYNVSVESILDYNGLNEKSILTIGD
                     EIIIPNVVKVVKPAPVQTVPPPSVQDSSELGNIYEHNKNIYVVKEGDTIESIAEDYGV
                     SAESIKNENNFNNDTVIQIGQEIKIPEGTDQDVEEDTTEASDASGTKGEYLDWFKDVQ
                     YIYDKGDKATVTDVETGKSFNVRRLYGRNHADTEPLTPEDTKIMKSIYGGSWSWNRRP
                     IILTIEDDEGVLRKIAASMNGMPHGGEDITDNGMNGHFCIHFRNSRTHSGSRSDPGHQ
                     KAVKIAAGLE"
     gene            26337..28286
                     /gene="glgB"
                     /locus_tag="PKI50_05800"
     CDS             26337..28286
                     /gene="glgB"
                     /locus_tag="PKI50_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNT03260.1"
                     /translation="MEGQPHSSYDAYLFREGNHYRSYGFLGAHFTNINGTDGAQFLVW
                     APNADAVHLIGDFNNWRSGANPMVKDDDSGIWSVFVPGLREWDMYKYEMHSRDGRVNA
                     KSDPFAFYSELRPGSASKLVSLGSYKWNDGNWMENRKRTNIYESPVNIYEVHAGSWRQ
                     KPDGRYYSYRELAEELVDYVKNMGFSHIEMMPLTEHPFDGSWGYQSTGYYSVNSRYGS
                     PYDFMYFVDKCHQEGIGVILDWVPGHFCKDDHGLRLFDGTTLYEYEDPKKGENYGWGT
                     CNFDLGKPEVHSFLISNAVFWFDIYHVDGLRVDAVASMLYLDYDREPGEWIPNIFGGR
                     ENLEAVDFVRKLNKVVFEYFPGVMMIAEESTAWPLVTAPAYLGGLGFNYKWNMGWMND
                     MLKYMKTDPSDRKRHHNLLTFSLMYAYSENYVLPLSHDEVVHGKKSLLDKMPGDYWQK
                     FANLRVFLGYMAAHPGKKLLFMGGELGQFIEWRYDHPLDWMLLDYEMHRGIQNYVKML
                     NQVYAREPALWELDHVFEGFEWIDADNSMQSIITFLRKGKRPEDTVVAVCNFTPAVYE
                     GYKIGVPYNCSYYEIFNSDRQEFGGSGFNSTLIFSAVKKEWHNRQQMIKINVPPLAVL
                     YLKPAAIPENERKMETVQLETAAAK"
     gene            28364..29515
                     /locus_tag="PKI50_05805"
     CDS             28364..29515
                     /locus_tag="PKI50_05805"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNT03261.1"
                     /translation="MKKKEWIAMILAGGQGSRLTLLTKKIAKPAVYFGGKYRIIDFVL
                     SNCSNSGIDTVGVLTQYQPLVLNSYIGIGSPWDLDRKKGGVTLLPPYVNERGGEWYKG
                     TANAIYQNINFVDEYDPDYVVVLSGDHIYKMDYSKMLDYHKETSAEATIAVIEVPWEE
                     TCRFGIMNTGPDGRIEEFAEKPRNSKSNLASMGIYIFNWDILKKYLVEDEADPESSND
                     FGKNIIPKMLNSGIRLFAYPFDGYWKDVGTIESLWEANMDLLKEDNRLNLFDPKWKIY
                     SVNAIKPPHFAGATADIKCSMVGEGCVIHGEVRNSVLFPGVYIGKDTKVIDTVVMPKV
                     MIGENVVVERAIIGLESVINNGCRIGDSTSGSITLIGEYEQLPQGTVYI"
     gene            29550..30668
                     /gene="glgD"
                     /locus_tag="PKI50_05810"
     CDS             29550..30668
                     /gene="glgD"
                     /locus_tag="PKI50_05810"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HNT03262.1"
                     /translation="MLDYMGIINLNEREDNIKGLTGHRPLAAVPIAGRYRMIDFALSN
                     LVNAGVQNISIFTQNKYRSLLDHLGTGKAWDLDRKNDGLFIMNPMYDYYNLGIYRGDI
                     ENFKCHMDYLNFSKQNNVIITPSNMICNLDYGEAIKQHNETGADITIIYKKADNCTVD
                     FQYCNTLEMDEYGRVERIDINFGNENECNVSMDMYIMKKSLFLDIVNSCVSTGYCDYF
                     REAVYKYLHKLKIFGYGFKGYLACINSAYSYYKTSMQLLNMDISKELFFKNGLIYTKV
                     KDEPPTKYKENARVINSLVANGCTIDGQVENSILFRSVKIKKGAVVKDSIIMQNCSIE
                     EESRVINAILDKSVRVSKGKRIIGYADNPEIIEKKAVI"
     gene            complement(30730..31092)
                     /locus_tag="PKI50_05815"
     CDS             complement(30730..31092)
                     /locus_tag="PKI50_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT03263.1"
                     /translation="MNFRHVTLTVKDLDASIHFYTEIVGLPVDRRYQPGLDTEIAFLG
                     TGETKVELICHRNQPDAIVGNAVSVGFEVEDILEKFESFKGTDISVVSDIIQPNQHVR
                     FFFVADPDGFRVQFLESL"
     gene            31309..33735
                     /locus_tag="PKI50_05820"
     CDS             31309..33735
                     /locus_tag="PKI50_05820"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNT03264.1"
                     /translation="MSVDKNKFKEDYLKKLMTMHAKAFKEATNNERYSALGSLIRDYA
                     AMDWVRTNEEYRRKNAKQVYYFSIEFLLGKLLGNNLVNMGLEKVCEEVLQELGCTLKE
                     VKSAEPDAGLGNGGLGRLAACFLDSMASLGIPGHGCGIRYKYGLFQQKIVDGYQVELP
                     ENWLREGNVWEMRKAGKAVEVRFGGKVAPEYKNNRLEFKHVDYETVLAVPYDTPIIGY
                     NNGTVNTLRLWSAENPEKDFDLSSFSKGDYMKAVEHKYSVESISQVLYPDDSFAEGRK
                     LRLKQQYFFVSAGLQSIVRSYKRTNDNIYDFDEKVAIHINDTHPAIAIPELMRILMDE
                     EGVDWDTAWKITTNTISYTNHTILSEALEKWQIDMFKPMLPRIYMIIEEINERFCGDL
                     WNRYPGQWDKISRMAILADGNVKMAHLAIVGSHSVNGVAKIHTEILKKQELADFYHFY
                     PYKFNNKTNGITHRRWLLKCNPELAQLLTDKIGGSWKKHPTDLIRFLKYRNDPEVQER
                     VRQIKQNNKLELTKIIKDEYSIEIDVNSIFDIQIKRMHAYKRQLLNALHIMYLYNCLR
                     ENPRLDIIPRTFIFGAKAAPGYKLAKQEIKLINTIADKINNDQSIENKLKVVFMEDYK
                     VSLAEKIIPAADISEQISTTTKEASGTGNMKLMMNGAITVATLDGANIEIRDEVGDEN
                     IIIFGMTEKEVLDYYRNGGYSAIEIYNSDPRIRTVADQLINGFFPVSSVEFLDIYNHL
                     LLHNDEYFVLKDFNAYAEAHKRVDETYRNKSEWMKMSIANIAHSGIFSSDRTISEYAS
                     GIWEVKRVIL"
     gene            33732..35687
                     /locus_tag="PKI50_05825"
     CDS             33732..35687
                     /locus_tag="PKI50_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNT03265.1"
                     /translation="MTDMMILHNSRREKYRFPFGAVPCKSTVRLGITVKASQPVNSAE
                     VIVSTEGGIEQRIALSAPEGQGEDYYANIEAPEKPGLLWYYFELVIGDKKYYYGNNER
                     FLGGIGGVYGTIPPRYQITVYRREASTPYWYKKAIMYQIFVDRFRNGREDCKVLNPKK
                     NSFIYADWGDTPFYIKDCEKGSIARWDFFGGNLQGVTEKLDYLKELGVSVIYLNPVFE
                     APSNHKYDTADYKNIDPMYGSSETFRDLCEKAGRLGISVILDGVFSHTGSDSIYFNRY
                     GSYPDIGAYQSAESPYYSWYRFNCSKDDYECWWGVDSLPNVNEMDPGYIDFIITGEDS
                     VIKHWMNLGAKGWRLDVADELPDEFIRLIHKTMKEKDKDSVLLGEVWEDASNKVSYGK
                     RRAYLLGEELDSVMNYPFRGIMIDFFTGKRDAEYVNEVLMSIYENYPLHNFYCNMNLI
                     GSHDVPRILTILGEAPPEKQLTQSQKESYRLSESQKALGIARLKLLALIQMTFPGVPG
                     IYYGDEAGLEGYSDPLNRGTYPWGGENRELLEWYKAITSIRNKYDVFSTGRWIPIYSE
                     GDVYGYVRRTEKGRDVFGRLCEDNTALVLVNRSRDFRYPLSLDISQWHRGVIYDILEG
                     GKAADIGEGILDVTLEPLQGKVYIAKL"
     gene            35708..37495
                     /locus_tag="PKI50_05830"
     CDS             35708..37495
                     /locus_tag="PKI50_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNT03266.1"
                     /translation="MSIKESILLRSNSIQTKKSRKDRKDKGVFYTPPQVVERITEKLL
                     SGVDLSANPYIKIMDPACGTGLFLIKAFEVLKRKFEENYEVVLDRNEELRGVLKKDGI
                     GSFILENNLWGADIDREALDAAAEMLMKLAGRESKVNLVCCDSLISGSTAQMSFFDDI
                     PPEEYQLWNSSYDYIIGNPPYIGHKQVSGEYKQVLQQLYKGIYKDKSDISYCFIKKGI
                     DLLKDGGSLSFVTSRYFMEGPSAAGLREFIAGHCTVTEVMDFYGDKVFRDAGVAACII
                     TLRKGIFDGETSVLKRRQGIKGNEPELFVPSDFEHFTVKRAELKDEGWVLLSPEKYEV
                     FSMAEAGGSLKLEEIADSYQGIITGCDKAFILNRSEIEENKIEEVLLKPWIKNSNIQK
                     HCIKPADKFLIYSDFIKDEKDFPNAVSFISRCRDRLLNRRECRKGIRKWYQLQWGRTN
                     EVFDTPKIVYPFKSASSRFAVDESGNYCSADVYSLKLKKEYEGRITLEYITAVLNSRL
                     LEFYFKCYAKKVSKDFYDYYPNTVLRMKIPMPAAVNPIGELASKLKNCKNEDARKAVT
                     YEIDREIYRLYGLSKKHIKIIEEDSMIYE"
     gene            37615..38322
                     /locus_tag="PKI50_05835"
     CDS             37615..38322
                     /locus_tag="PKI50_05835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNT03267.1"
                     /translation="MLLLAAAVAPSAALLYYFYTRDKYEKEPRKMLVKAFLIGGGLVV
                     PVLFIELSLNIFDIAEANLLAAGYTAFVVAGLVEESSKFLFFILYIWKHRDFNEMYDG
                     IVYSVFISLGFATVENLAYVLSTGFSTAVVRSLTAVPAHALFAVVMGYYLGIARFAKT
                     RYRRKYIILGLIIPVLLHGIYDFILLSQKLYLLVLFIPYMLYLWKRSLGHVDELVESS
                     RFKDRPEEYDDDTEGRV"
     gene            complement(38326..39420)
                     /locus_tag="PKI50_05840"
     CDS             complement(38326..39420)
                     /locus_tag="PKI50_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HNT03268.1"
                     /translation="MRLTYPHMGEAYIAVKSLMDDIGVESIIPAFTTNSTLRLGSSLS
                     PEAVCLPYKIMLGNYINSINAGADTILITGSCGPCRFGYYGPLQKQALWEAGYKDTDI
                     IILDPPREGYRKFLEALKKVAGKNSGRIIPSFINAYKVCCELDSMNELLYYTRPRAFK
                     KDELRSIMHKFKSDVMAVKGSGQMLELIHDTKNRIRGIDIDKSFKPLKIGIIGEIYTI
                     IEPFSNLHTEERLGDLGVEVHRSMTVRDWARNNVIFPATGRKGYKLEKKLSKPYLPAL
                     IGGHTQQCIGHAVHYAKTGYDGLVQIYPMTCMPEIVSRSILSQVEKDYNIPILYLIVD
                     EQTGEAGFQTRLEAFVDYVNARQEARDTRH"
BASE COUNT        11973 a         7314 c         9486 g        10677 t
ORIGIN      
        1 tgtggaatac gggcaaacaa tatgcgacat ttatagtttc ctataaatgt cgcattcttt
       61 aaaatgaggg ggaggaataa agagtaatag cacttattac tatctatatt cctattatat
      121 tcaataagga tacataaaga tatagtctga agccagttgt tacaaaatgt ttacaacttt
      181 ggagcatttt atgtacacta gttatcagta tctgtctccg cctaattcac ccagcaggct
      241 tccgagtcct ccaagcattt tgccgctgct tctgtcctca ggcgaggatt ttgttgcgtt
      301 tctgaaaagt gttctggcaa gggatgtgat gttcatgctt tggattaata cttttccgtt
      361 tcctgaaaga gttgcaagga aaaggccttc accgccgaac agaaggttct ttgcaacctg
      421 ccccacactt ccgcctaccc attcaatgtc atagtcaata tttttatcaa aagcaaccaa
      481 agcaccggta tctacctgta cttttccgcc gtaatccgcg ggattgattt ctatgaaatt
      541 accgcaggcg ccaatgaaaa gggttcctac atcactgaat ttttccaata tgaagccttc
      601 accgccgaag aatccggctc ctattttttt tgtaagtgcg atatcaaagt ctacagtatt
      661 ttctgcagcc aggaagccgt ccttctgaac gtacagcacg ccgaaatcct caatatttat
      721 tgcttttatc tctcccggaa tagtctggga gaaggttacc aacccgctct ctccatgggt
      781 tacattaaat acctgaaaag ctgcggattc tcctgcaagc tttcttttgc caacctgaac
      841 agcggtatcc atgaacttgc ccataacgcc tccgccgctt ttttcttcga agtgcccttt
      901 tttgtcacct acgttgaagg aagtctcaaa cttgatattg tcggttttcc acaggaattt
      961 tcctgcctcg ctgtacattg tctggtctgc cccaagttcg acaactactt gctgcatggc
     1021 attgccgact attttgtaat taagcataca tgacccacct ttcattttaa taatacggta
     1081 tttagtgctt taatcactgt aatacaatat ccgcaatata tatatattgt attataaaac
     1141 ttttggaatt tgaatacgaa attgctatat tttagttaat atagcaaaaa tgaggtataa
     1201 taaaagcaat tattatatat ttgtctaaaa gcccgtgtga ttatttgcct cgtaaaatgc
     1261 acatggacgg atgtcataaa ttttgaagaa tttatgatat aatcatctat atagattcaa
     1321 tgtaatttgt ttagtgcgta aaacacgatt ggaggttttg gttatgcaag aaatgataac
     1381 tctgacattc cccgataata ctaaatgcga atatcccaag ggtatgaaac ttctggagat
     1441 tgcaaaagat tttaaacagc attttaattc agatatagtc ggagcaatag taaataatga
     1501 actaaaggag ttgtgggttg agctgcggca ggactgcagc ctgcagttca tcgacaggac
     1561 tactgtgtat gggaacaggt tttactccag gagtttggct tttcttttta taattgcggc
     1621 aaaggaggtc tttaaagact gcagggttac ggtagagcat tccctcagca agggccttta
     1681 ttgcgaggtt cataaggata agccattggc aggggaagat attgcaagaa ttgaagagaa
     1741 aatgcgggaa ttccggaata tggatatacc ctttgttaaa aggaagatga ccagtaccga
     1801 ggctatcgaa atttttacac gaactcagca gtttgataag gtaaacctga taaggtatcg
     1861 aaagaaaaag catataaata tttacagctg tcacggacat tatgattatt tctatggcta
     1921 tatggttcct tccaccggat atctcaagat ttttgaactg aaagccaatg gaccgggact
     1981 tatccttaga tatccggaaa ggtcagagcc caataagctg cctgagttca aggagcagaa
     2041 gaaactcttc agcatattca gagagtttga agaatgggga aagattctgg agctggagaa
     2101 tatcagccat ctgaatgatg cagtcatgga cggcagaata aatgacgtga tacgagttgc
     2161 ggaagggctt catgaaaaaa agattgcaca gattgcggat atgattgttg cttcaggaaa
     2221 taaaaagctt gtgcttattg cgggcccgtc atcctcgggt aaaacaacct ttgcggagcg
     2281 gctgtctgtt cagcttaggg taaacggact gaagccggta gcaatatcca tagatgactt
     2341 tttcaagaac agagcggata ctcctatagg tgaagatggc gaatatgatt ttgaaactat
     2401 agatgcaatt gatatagcaa cctttaacga tgtcatagcg aggcttatta atagtgaaga
     2461 agtggaaatg ccggtattta acttccatac gggaaacagg gaatggaaag gcagaaaact
     2521 gaaaataggt aaagaacaga ttctggtggt ggagggaata catgggctga atgaaatgct
     2581 cacacagtgc atcccgaagg agcagaagtt caagatatat attagtgccc ttacgcatct
     2641 cagcatagat gaccataaca ggattcccac atccgattta aggctcataa gacgtatagt
     2701 aagggattac cagttcagat ccgccgatgc gctttccaca attaagcggt gggattcagt
     2761 aagaagaggt gaggaaaaat acatttatcc cttccaggag gaggcggatg ttatgttcaa
     2821 ctcatccctg gtgtatgagc tggctatgct aaatcagatt gtactgccgc tgcttgagaa
     2881 gattgacaac ggagtacatg agtatattga agcaaagaga attaagaaat ttttgcaata
     2941 cttcctggaa gcagatatga atgcaattcc ttccaattca atactgagag aattcattgg
     3001 cgatagctgt ttttttaaat aaaaccggtt gacatccaaa agctgcatta attgcagctt
     3061 tttttcttaa tattattatt atttaagcaa aaaatattaa cgcagaacta aaagagaagg
     3121 cagcttgtga tgggctatct cagatttttt attgtatagg aaagtacgct ttcctataca
     3181 ataaaaaata tggaggaata acagcctatg agaagaaaaa atatcgtaat atcggccttg
     3241 gtattgtgtt ttgcattgat gtttagcgcc tgtgctgcaa gaaggcctct tcagacaccg
     3301 gcacagccgg ggcaggatac tgcaggccgt accggcacat atacaggaat tggacccagc
     3361 accacaaatc cctatgacac cggatatggt gaattccctt catattacgg aggttatggc
     3421 ggctatggcg gctataacaa cggtatgtat gggggatatg gaaatgacta caggaacatg
     3481 ggaggcagca attattctcc taacgtagga atgaacaata caaggcttaa tacaacccaa
     3541 gcccagagac ttgcaagagc ctgtgaagca gtgcccggtg tcgataatgc aacttgcgtc
     3601 atatcgggca acacatgcta tataggcgta gatactgatg gtgatcttac cggcagaaat
     3661 atcgcctacg gaaatgcaaa cgatctgtca gcggtaaaac gacagtgtgc ccagaaggta
     3721 aaagcagcta atccgcagat tcagactgta tatgtcagca ctgacacaaa tttctttgaa
     3781 aggttgagaa gaatcagcga tggtgccgga aacggtaatt taggagataa cttcagaaac
     3841 gagcttaacg agcttgtaag ggggctaact ccggaaagac tgcagtagca atagattcag
     3901 tagcgttccg cagtaatgcg gaacgctatt gttttcctgc aaattaagtg catcggcaga
     3961 tacatttttg ttataatata gatgaaagat agttttcctg gtattatttt acataggtac
     4021 atacatttaa atgctctgca cggaatcctt catttattct attcacatat ttcatctttt
     4081 tgattacaat atattggtgg gataaagact cgggaggtaa tatttgtggg atttacaaat
     4141 tcatttaacg attttttgtt ttctgttgtc ccgtttttta tgtttattat tttcgtcata
     4201 gtgaccatga ccttcataac atcctttacg gagggattgg gacaatatct tagaaacaaa
     4261 agcgctccga agaaaaccat cccggcaagg cttgcagcga agaggacaca tgattggggc
     4321 ggatatgggg atacggatat ccgtatcagc tattatgctg cctttgaaac tgaaaatgga
     4381 gagcggctgg agtttcctgt aagcagcagt ttttccgaaa tgtatgcaga aggcgatacg
     4441 ggaatgctga catataaggg tacaaaattt attgacttca agatagaata attctggggt
     4501 aaaactgtat caaaccgtta acgaggtgtt tattatgaaa gcaaagagac taaaaaccgg
     4561 agatacaata ggcatagtgg ctcctgcaag cccatcggaa accttaaaag cagaaagggc
     4621 aatcaaacat ataaccgata tgggatattc agtcaaggcg ggcaaatcgg tttacagcag
     4681 cagggggtat ttggcggggg cggatgaatt gagggcttcc gacatcaatg atatgtttgc
     4741 ggatgatgag ataagtgctg tattctgttt aagggggggt tacggttcac agcgcatatt
     4801 ggatttggtg gacttcgaaa ggattagcag taaccctaaa atattcatgg gctatagtga
     4861 tattacagcc ttattgaatg caatttacca gaagtgcggg cttattactt ttcacggccc
     4921 tatgggagga gattttgcag gggggctgga aaagcgtaca aagagagcta tgaaaaacat
     4981 tctggaaagc accgagccgg caggagagct gccgaatcct gtaaccccgg gggttgtaga
     5041 agagggaaaa gcatcgggta aattggcggg gggcaatctt tcaatagttg cggcttctct
     5101 gggtacgcct tacgaagtgg acacccgaaa caggatattg ctgctggagg atgtatttga
     5161 ggaaccctat agtgtagacc gaatgctgac acagctcaag ctggccggca agcttaagag
     5221 tgccgcagga attatactgg gggattgggg gaactcggag ccggaggaac cagagaaaag
     5281 cctcagcctt gaagaggttt ttgaagacat tttcaagggc ttggggaagc ctgttctgaa
     5341 gggcttgaaa ataggacact gcaagcctaa cttcacagtt cccataggcg cagaggtatc
     5401 aattgatacc tgcagcaaaa ccgtatgcat acttgaatcg gcagtggaat agcagaaaaa
     5461 ttcaaaaaaa gttgattaag gtaacagtta tgttataata taggggatta agcaacaact
     5521 aatatttgcg aatggattat agtgttttgt acagtttatg acaaagatac actattacat
     5581 aattaaggag gaaacaaatg aactcaaaat tagagaaact tgaaaacaat gttgcgacat
     5641 tggagatatc agtatcggct gaaaagcttg aagaagggat aatgaagtca tacttgaaga
     5701 atgtcaagaa attcaatata gccggtttta gaaaaggaaa agcaccaagg aaaataatag
     5761 aaaggcatta tggtgaagcg gtattttatg aggatgccat aaatatcata tgtcctgacg
     5821 catatgatga ggctgtcaag gaacacaatc tggagcctgt cgaccgccct gatatagaca
     5881 tagttgatat tgaaagcggt aaaggcttgg ttttcaaggc tgccgttact gtgaaacctg
     5941 aagttgcatt gggacagtat aaaggaatag aagcagaaaa aaaggagtat aatgtaactg
     6001 atgaggaagt ggataaggaa attgagacaa taagagaaaa gaatgccaga ctcatagata
     6061 taagcgacag gccggtaaag aacggagaca taacaattat tgactttaag ggctttgttg
     6121 aggataaaca gtttgcgggc ggcacttcag agaactatag tcttgaggta ggttccggac
     6181 agttcatacc gggtttcgaa gagcagctgg ttggtgcgga gttaggtaag gaaatagatg
     6241 ttaatgttca gtttcccaag gaatataata gcgaagagct ggcaggaaaa cctgccttat
     6301 tcaaggttac cgttaaggaa ataaaggaaa aacagttagc tgagttggat gacgaattcg
     6361 ccaaggatgt aagcgaattt gatactctgg atgagctgaa ggaagatata aggaaaaaga
     6421 aggaagagca gtacaaaaga ctggaaaagc agcagtatga ggatgaagtg ataaacaagg
     6481 ttgttgagaa tgcaactgtt gatattcctg aggtaatggt agatgcacaa gtaagaataa
     6541 tgctgagaga ctttgattat cagctgagat atcagggctt gaatcttgaa gcatacctta
     6601 aatatatgaa tatggatatt gaaaagctta aggaatcata cagggataca gcaaaaagca
     6661 gagtgaagag ccagctggtg ctggaaaaag tggcggaagt tgagggaatt gctgctgcag
     6721 aagaagattt gaatgccgaa attgagaaga cggcaaagca ttacaatcag gatttggaaa
     6781 aattcaagaa aactttgaaa gaagacgaaa taacatatat aaaggatggg atagtcatcc
     6841 agaaggccat tgacttcctt gtcgataaca gcgtggccat atagttattt tcatggaaag
     6901 ggaggatacc tatgagtttg gtaccgatag ttgtagaaca gactaacaga ggagaacgtt
     6961 cttacgatat ttactcaagg cttctgaagg acaggatagt ttttctgggc gacgaagtca
     7021 atgatgttac agcaagtctg gtggtagcgc agctcttatt cctggaagct gacgatccgg
     7081 ataaagatat caatttttat ataaacagcc ccggaggatc aataacggcg ggctttgcca
     7141 tatatgatac catgcagtat attaagcctg atgtctctac catatgcgta ggtttggcgg
     7201 caagtatggg tgcatttctg ctggcagccg gagcgaaagg aaagagattt tgccttccga
     7261 acagtgaggt tatgattcac cagccttccg gcggagcgca aggcaaggcc accgacgtgg
     7321 gaatatatgc agaaaggctt atcaggagcc gcaagaaatt aaatgagatt ctcagtgcaa
     7381 aaaccggaca gccgatagaa aaaatagaaa gagatgttga aagggattat ttcatgtcag
     7441 cggcagaagc aaaggaatat gggcttattg atgatatcct tgtcaagagg aagtaaagtg
     7501 aggtgatatc atggctaaat ttgatgataa aaagcagctg aaatgttctt tctgcgggaa
     7561 aaatcaggag caggtcagaa gattaatagc agggccgggt gtgtatattt gtgacgaatg
     7621 tattgagctt tgccaggaaa tcattgacga ggaattctat gaggaatttg atacagagct
     7681 gcaggatgtt ccaaagcctg cacagatcaa ggagttcctt gaccaatatg ttattggcca
     7741 ggaagaagct aaaaaagcat tggcagttgc tgtatacaac cattataaaa gaataaactc
     7801 cgactacaag atagatgatg tagagcttca gaagagcaat atagcactta taggccccac
     7861 aggctcggga aagacattgc ttgctcagac tctggccaag atgctgaatg taccttttgc
     7921 catagcggat gcaacttccc tgactgaagc cggttatgtg ggagaagatg tggagaacat
     7981 acttctgaag ctgattcaga atgcggacta cgacattgaa agagcacaaa aaggtatagt
     8041 gtatattgat gagattgaca agattgcaag aaagtctgag aatccttcaa taacaaggga
     8101 tgtttccggt gaaggcgtgc agcaggctct tctcaagatt cttgaaggca ctgtggcaag
     8161 tgttcctccc cagggcggaa ggaagcatcc tcatcaggag ttcatacaga ttgatactac
     8221 aaatatattg tttatatgcg gcggagcctt tgacggcctt gagaagataa taatgaacag
     8281 gacaggtaaa aaatcaatgg gctttgttgc ggatatacat tccaggaacc atcaggatat
     8341 cggagagatt cttaaaaaga taatgccgga ggatctgctg aaattcggcc tcataccgga
     8401 atttgtcgga agaattccga taatagtgac gttaaatcct ctggataaag atgcactggt
     8461 gaagatacta accgagccga agaatgctct ggttaaacag tatcagaagc tttttgagct
     8521 tgacaatgtg gatctggaat ttgaagatga cgccatcgaa gagattgcgc aaaaagcttt
     8581 ggacaggaaa acgggagcca ggggcttaag ggcgatattc gaggagaata tgctcaatgt
     8641 aatgtttgag attccgtcta gggatgatat agataagtgc atagttaaaa gagagactat
     8701 attggagcac aaggagcccg atatagtata ccttgagcct gggacaaaga agactgtgaa
     8761 aaagcagccc aggaaaggcc agaaagcaag gaaaggtacg gcataaatac aagaccggtt
     8821 agagcaaccg gtcttttaat ttgggattgc cgctcctggc tgctgctgtt cgaatgcatg
     8881 gttatgaata ctttcccgcc ttcaatataa taatactatt gacactcaaa tatgagggag
     8941 gaaaatgcca tgtcacaatt gcttattgct atccagcttt tttttgctgt tgtaatagga
     9001 atgtacttct taaaccttct gagaggccaa cagggaaaca aagttgctat agaaaaagaa
     9061 tcgcgaaaag aaatagaaaa actgcagcat atgagagata ttcagctgac aaagccgctg
     9121 tctgagatca cccggccttc tgatttcagg gagataatag gccaggagaa cggtataaag
     9181 gcattaaggg cggcaatatg cagcccaaat ccacagcatg tgataatata tggccctccc
     9241 gggataggga agacggcggc tgccagactg atactggagg aggcgaaaaa gacgccgaat
     9301 tgccccttcc gccaggatgc aaagtttgtt gaggttgatg ccacaacggt aagatttgat
     9361 gaaaggggta ttgcagatcc tcttataggc tcggttcatg accccatata tcagggagca
     9421 ggtgcgatgg gagtagcagg aattccacag ccaaaagtcg gcgcagtaac caaagcccat
     9481 ggagggatac tcttcataga cgagattgga gaactgcatc ctatacaaat gaataagctg
     9541 ctcaaagtgc tggaagacag gaaggtattc ctggagagcg catattacag ctcggaagat
     9601 gccaatattc ctttccatat acatgatatc tttcaaaacg ggctgccggc tgacttcaga
     9661 cttatcgggg ctaccacaag gggtccggag tcaattccgc ctgcactgcg gtcaagatgc
     9721 ctcgagatat atttcagggc actgacctct gaggaaatcg ggatagtgac ctccaatgcc
     9781 ctaaacaaga tcagatttga atgtgaagaa ggggttcccg agcttatagg gaagtatgcg
     9841 acaaacggaa gggaagctgt aaacattata caaacagcag tcggagttaa tataaatgag
     9901 aaaaggaata aagttactat aagcgatata gaatgggtgc ttaacaacgg acagtacagt
     9961 ccaaggccgg aaaggaagat aaatgatgaa ccgcaaatag gctatgtaaa cggacttgca
    10021 gtatatgggc caaatatggg cactcttatg gaaatagaag caattgcggt aagagccggc
    10081 cggggaaccg gacggcttct ggtcacggga atagtcgaag aagaggaaat aggctcaaga
    10141 gacggaagaa ccattaaaag aaaaagcatg gcaaaggctt ccaccgacaa tgtaatcact
    10201 gcaatcaaaa aatatttggg catagatccg ggggaatacg atattcattt gaatttcccc
    10261 ggaggcacac cggtggacgg cccttctgcc ggtattacaa tagcagtggc gatatattcc
    10321 gcaataacca acagctacgt agacaaccgg attgcaatga cgggagaagt atcaataaga
    10381 gggaaagtaa agggcgtagg aggtattatt cccaagattg aagctgctgt ccgcgcaggg
    10441 gtaaaaaagg tcatcattcc aaaggagaat tggcaggaga tattcagtac ttatgatatt
    10501 gaagtaatat cggtggaaag tattgttgaa gctgtcaaca tagccaccat gaggcaggcg
    10561 gaagtgaagc acctgaaggc tcagtcttcc gagggaaata tactaacggc atcaaaaatt
    10621 gccaaagctc cttcaatgca ggctatgagc gatataaaac agtaatggaa agaaggacat
    10681 ctttattaaa aatattgata aaaaatttat gtaaagatat aatagattag acacatggta
    10741 ttacaggagt tgataaaatg cagaaaagaa aagaagatat taagagattg ccgttgctgc
    10801 cacttagagg cttaagtatt tttccataca tggttttgca ctttgatgta ggcagggaga
    10861 agtcaataag agcactggaa gaggctatga taaatgatca gcttatattt cttgttactc
    10921 agagggatgc tcatactgat ttgcccacag tgaaagattt ctatgaagtt ggtacaatat
    10981 caaagataaa acagcttctg aagctgccgg gggacaccat aagagtgctt gttgaaggaa
    11041 tcaacagagc agagataagg gatataattt ccgaggatgc ttttttcctt gtggatgtgc
    11101 tggaacatca ggatgtacag ctgtcgggag atttggagct tgaggcatta agaagaagtg
    11161 taatggaggt tttcgatgaa tatattaaag caaatcccaa ggttgcaccg gaaaccctta
    11221 tcacggtatc agacatagat gatgcgtcca ggtttgcgga cgtgatagcc tcaaatctca
    11281 ccattaagat agaagagaag caggaaatat tgtccttgtt tgatgtaaag gaaagaatgg
    11341 agaaaatatt tgaaatactc tccagggagc ttgaaatact tgaaatagag aggaagataa
    11401 actccagagt cagaaaacag atagacaagt cccagaagga atattatctg agggagcagc
    11461 ttaaggctat tcagcgggaa ctgggagaaa aggaaggaca cggagaggaa attgagcaat
    11521 atgctgaaaa gctttcaaag ctcgaagtac ccgatgaggt aaaggaaaag gtggaaaagg
    11581 agctggatag gctgtccaaa atgtcccagg gctctcccga ggtaggcgtg acaaggactt
    11641 acctggattg gatttttgat ttgccttgga atactctgac ggaagataac cttgatataa
    11701 agaatgcaag aaaggtgctt gacgaggatc attacggact taaaaaggtc aaggagagag
    11761 tgcttgagta catggctatc aggcagcttt ccaacaatat gaaggggcca atattgtgcc
    11821 tggtcgggcc tccgggagtg ggcaaaacct ccatagccag atccattgca agggctatgg
    11881 ggaggaagtt tgccaggatg tccctgggcg gagtccgtga tgaagcggag ataaggggac
    11941 atcgcaggac atatatcgga gctattccgg gccgtattat atactccata aagcaggcag
    12001 gttcaaggaa tcctttgttc ctcttcgatg aaatcgataa actggccagt gacttcagag
    12061 gggatccggc ttccgcactg cttgaagtcc ttgattcgga acagaacaag gaatttaggg
    12121 atcactatct ggaactgccc ttcgatcttt caaaggttat gttcttaacc accgcaaatt
    12181 cgacggatac catacctccg gcattgttgg acaggatgga ggtaatatat atttcaggat
    12241 atacggaaga ggagaagctt catatagctg ctgactatct ggtgcccaag cagataaaag
    12301 agcattcact gaaggatgaa agcgtttcta tttccgaagc tgtcataaga gcggttatta
    12361 acggttatac aagggaagca ggggtaagga acctggagcg cgaaattgca aatattatta
    12421 gaaaatgcgc tactcaaata gtggagaata acaaaaaaag agtcagagtt actcccggta
    12481 atctgaaaaa atatctcggg attcaaaaat acaggcatga tttgataaac cagaagaacg
    12541 agataggtgt agtaaccggt ttggcctgga cacctgtcgg cggagataca ttgttcattg
    12601 aggtttccgt aatgcccggc acaggaaagc ttgagcttac gggacatctc ggagatgtta
    12661 tgaaggagtc tgcaagagcg ggcttaagct acattcgctc cagatgtgag gaatttagta
    12721 ttgataaaga atttacgaag aatatggata ttcatataca tgtcccggag ggtgccacac
    12781 caaaggacgg cccttcagca ggaataacca tgacaacagc attggtatcg gctcttgcaa
    12841 agattccggt gcacagaaac gtagcaatga ccggagaagt cactttaagg ggaagggtgc
    12901 tgcccatagg cggtttgaag gagaaggtgc ttgctgccaa gcgtgcaggc ataaagaaag
    12961 taattcttcc ttatgagaat gaaaaggaca ttgaggaaat tccggatacc gtaaagaaaa
    13021 acatgacctt tgtgctggta tcagatatgg acgaggtcct gtcccatgca ttgataaggg
    13081 atgagaaaca tgataattaa gaaatgtgag tttattaaaa gtgcagttca ccaaaaccag
    13141 tatccagagc ttgatctgcc ggaaatagct ttttccggca gatctaatgt cggaaaatcc
    13201 tcgcttatta attcgcttct taatcgtaag aagcttgtta agacaagctc tagcccagga
    13261 aagaccagat tgattaactt ttttatgatt aatgatgagt ttgttctggt tgatttaccg
    13321 ggctatggat atgcagccgt gtcaaaaagc cagaggcagg cctggggtaa gataattgag
    13381 aattatctga ggtacaggga aaacctgaag aatgttgttc tgctggtaga tatacgccat
    13441 gagcccacaa ctgatgataa acttatgtat gattttatta agtattacag aaagtctgta
    13501 actgttgttg cgacgaagct ggataaaatc acccggtcat cagtgaacaa gaacttgaat
    13561 ataattaagc aaaaacttga tattaatcaa aatgatattt taattcctta ctcctctgag
    13621 acccatgtgg ggagagagga actctgggag gtactgctga cctggaagta ggcttatttg
    13681 attggggggg acactattgg aggtatatga ggcatttatt attattctta cttttttgct
    13741 gcttaatgtc ccgattttta tctcactgta cgaatatata ttcaaggata tgagcttaat
    13801 cgcaaagttt gccgtaggag tggtttattg gtctgctgcg tttttgattc atgaactggc
    13861 tccttttatc ggggtgctta ttctgctttc caaagttcac aggaaagcag aggttgaaat
    13921 tcatgaaagg aatatcaata tatggtcggc aacagccggg gatattattt ttgttgccgg
    13981 tgccaccctg gtttttaaag cggtaatcaa tcagataaat aagttatatg tgtacatttt
    14041 aaacatatat ttcaatatgg acgccaaacc tcaagaaatt gtagaagaat ttgccggcgg
    14101 agagatgtac tacaaagtga tgctttttat tttggtggta atattggcac cttttgtaga
    14161 ggaatatatc ttcagatatt atatttatga taagctttta ctgaaaagta tgccccatgc
    14221 ggcagctgcc atcatatcaa cattaatttt tactctgctg catttcaata tatcaggcat
    14281 tcccaccttt ttggggttgg ggctttactg tacattcata tatgagagaa agggctttta
    14341 tggtgccgta accgcccacc tggtatcaaa ccttgttact gcggcaatgt taattgtata
    14401 ggaaaggata ctttcctata caatggggga tgcaaggggg gaagccccct gccgataaag
    14461 gagggtgctc agctgcagag cagcgtcgaa gggaaccata gggttcccta gcgaaaaagt
    14521 gcggcgcgag acgcactttt aattgtatag gaaaggataa gtattcatat ggaaattgaa
    14581 tttcatagag tattattgga cgcattgacg gaagccataa tgagaaagtc ggtagtgact
    14641 gtgaagaata tggaaaacga cagtataata gtggttactg ccaccgaaga tgaaataata
    14701 atatccctga gcaggattcc ttccatgatg aatgatgtaa tatcagcggt atctgactgc
    14761 ctgggcaggt gccctgacat gatatacgac agagccagtg tattgaagga cgatattgtt
    14821 atatactata tggtttggct caacaataat gaaggaggaa gatccttcgt ggagaaggag
    14881 ttcgctgtgc ctcagaacag gataacctat ttgagccaaa aggctcttgg ctatctgcaa
    14941 aatatgaatt aataatatat catcaagcat tgctgaggaa gaacaaagat tggaaccata
    15001 ctctgcacac aagaaaagtt accatacatg aattggaata aggcctaagg ccttatttac
    15061 ttatggttcc aaattctgta taatatctat ggcggactca atattttgtt tgtgggggta
    15121 ataaaatgct taaaaagcta aagctgtttt ttatcaaacc aacggagctg ttcagggatt
    15181 acatcgagaa gcctgtctgg gcattgaagc tgttcgttat agctcttgtg ataggcttat
    15241 atacctatgg aaccaagatt cttggcaagg atttgtttat tgaaatgatg gaagaaaagg
    15301 cagcagctat gccgcctgaa caggctgaag cattaagggc atcaattgct tttatgaata
    15361 cgcctatgat gaacctaatt tctgctgttg tagcagcaat atcaacagtc gccatcattc
    15421 tgctggttgc acttgtatat aaggcattta tcagagcact caagggtgaa ataaaataca
    15481 aacaggttct ggcagtctat actctggcat atatggcatc tattgttggt atagcggtaa
    15541 agtttgccta catgtatttc acaggcaacc ttctatatct ggatttaagt ccaacattta
    15601 aagacgtact gcttaacaac cttgatccat ttgttatctg gcagtcaata cttatggtat
    15661 tcggaatatc tgcagtatca ggcattcctg aaaagaagag catcattata gtagcagtaa
    15721 tgtggctggt tgctctggca atatcattcg gctctgtgat gttggctaat taaacttagt
    15781 aatatttcct ttttgttaag ctcgggctct tcaataacct tttctaaaag gctgttgagg
    15841 agttctttta ttttcttccc gggtttaata cccaatgcaa taaggtcatc accgtttact
    15901 gcaagagatt tcaggcttgt acactggcct ttttccttga tatccttata taattcccat
    15961 atttttatga atttcccggt gcgttcctta agaagtccgg ggttctgcgc ctttttatcc
    16021 gcctcaatga ccttaagaag gctttcaaac atatcatctc caactctgga catggctttc
    16081 ctgactgatt ttgcatcggc ctttatgtgc atatcatggc atcgcaccag cagaagtatt
    16141 ttgtccatgg actttttgtc aaatctcagc cttgacatgg ctgttccgga aagcttgaca
    16201 cttagctgct ggtgattata aaaatgatct attcctttgc tgtctgtggt ttttgtaccc
    16261 ggctttccta tatcatgcag cagcattgcc catctgagga tcttgtcctt ttcaacatgg
    16321 gctactgagc gcagcgtatg ctcagctaca ttatacgaat ggtagggatt gttctgattg
    16381 gttataaagc agggctcaaa ctccggcaga gtatatttta tcagctttgt atccttcagc
    16441 agcgaaaagc aaagggggtt atcggataat agtatttttg taagctcatc tcttatacgc
    16501 tgggggctta tgcatcctat cagactgctg tttatttcaa tggatttcaa cgttttttca
    16561 tcaacagaaa atccgagttg tgcggagaaa cgtacagccc tcagcatacg aagggcatct
    16621 tcactgaacc ttctttcagg gtctcccact gtccttataa tgcctttcct gatatccgcc
    16681 acaccgccaa agggatccac aattccggct tcagggctgt aagcaatggc atttatggtg
    16741 aaatcccttc ttgaaaggtc ttcttcaatg gaagaagtaa aggttacagc cttgggtttc
    16801 ctgaaatcag cgtattcacc gtctatgcgg taagtggtaa cctcaaagag gcttctctca
    16861 attattacag tgacggtgcc atgcctgatc cccgtatccg cggttttttg gaataattct
    16921 tttacctctc cgggtaacgc atcagttgca atatcccagt cttcgggcac tttcccaagt
    16981 atgctgtccc ttacgcagcc tcctacgatg aaagcttggt aattgtgctc atgcagctta
    17041 tccagtataa attgaacctt ccggggtatg gggatttcaa tatcgtattt catataccgt
    17101 ccctccgttt ttatcctcca agggtcacat cctgatactg ataccactca agggctttat
    17161 acagatgctc cggtttaaaa tccggccaca ggtcatcgac aatataaaaa tcagcgtaca
    17221 ctgattgtat aggcagaaaa ccgctgagcc tgcgtcttcc gccccatcgg ataatcaggt
    17281 tcactcttga aatgtcggca gaggcaatat gctccggatg gttcaaatcc cagttccatc
    17341 cataattgac caggaagttg atttttataa gcccccgccc gaactttgcc ctcttggtat
    17401 aaggaagaag ctcttgggga aacattgggg agctggtatt cccaatgacc aatagctccg
    17461 catccttccc tgtaagttcc attacagcat cgacgcaagc ttttctgaaa gcttgtgtct
    17521 gtatggaggg cctcctggta ttgtcttctg taaagccgta tagggttaac tcttttattc
    17581 caagctcaat acatagtttg taaagctcaa agcccggttt tattccgtgg cagtatccat
    17641 cctgcttctc taagcccttt cccagggccc atctcctgtt tccgtcggga ataactccaa
    17701 tgtgcatcgg aagcctttta aagctgggaa cgctcatagg ttcacctcgc ataattaatt
    17761 cgtattgctt tattattccc gatatgtaaa gaattagcct tatatgcttt atataattat
    17821 agcttttttt gcggcagcag tacagcataa acctttgcac ttgcctgata agaagaataa
    17881 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccgcgtc cccatgtccc
    18001 cgtcgcatta ttcttcatat attacgttac agttaaagga aaatcgcaaa ctgtgtagaa
    18061 tatatgaagg gaataaatat gtgaggggaa ataaatgaag aagtttatca gaatttttgc
    18121 attggtgctt ttaattatgt ttgctgtttc aggctgcttt atcaaggggg cggatctccc
    18181 tgaagatatg gtcggaatgc cttcagaggg ttacggcaat accctgggaa atataacaaa
    18241 cggcggtgtt gtggcatttc aggaaggctg ggtatattac tgcaacggca atgatgatgt
    18301 aaatatatac aaaatgagaa taaacggatc ggaaaaaacc aaattaaaca gtgataagtc
    18361 aatgttcctg aatgtgcagg gagactgggt ttattacagc aatattgaag aaacggaaaa
    18421 aagcagcata tataaagtaa aaacagacgg cactaaaagg acaaagctga ctaacgactc
    18481 tgcatccttc ataaatgttg tagatgaatg gatttattac agcaatgctt ccgataacag
    18541 gcgaatttac aagatcaaaa ctgacggtac ggaaaaaaca ctcatcgggg acaacttttc
    18601 ctcatatttg aatgtgcatg atgggtggat ttattacact aacggagacg ctaaaaagcg
    18661 gatatataaa gtcaatattg aaggaacgga aaatgtcata gtgtcggagg attcggcagc
    18721 atatataaat gtggacggag actggatata ctacagcaac ggctctgaag ggaacaagct
    18781 ctataaaatc aggactgacg gtactgagaa tactgagctt acaaaggaag aagccctctt
    18841 cataaatgta tcggaggaat gggtatatta tagcaatgga tataacggca acaggatatt
    18901 caggataaaa aaggatggca cagataaggc aattctgaac tacgaaagct ccaagtttat
    18961 aaatatcgct gacggatggc tgtacttcat tgttgattct gcctcataca gattatttaa
    19021 gatgaaaagc gacggtacaa aaatacagat ggtagaaaaa gtagaaaggt aagaagtgcg
    19081 cttcttacct tttaaactat caaatccaca tgctgcaccg atccggcagt accgttctca
    19141 tgaaggaaaa tgccggtttt ctgaatgact ccgttttcct tgttttcttc tcctctcaag
    19201 ctgaaggccg ttcctgcgct gcccaggtat attgcgccta tacccttctc gccgagagca
    19261 aaaagcgtgt catttccata ttcgtccttt gtccatattc taagtctgtc gaaaattatg
    19321 tcgttttcgt ctatccatcc attttcatcc aaatcatatt gtgataaatc agcaaagccg
    19381 tcaccgcttt gagggccgaa aagctcgctt ccgtcattta ttttcccgtc attgttgaga
    19441 tcgagagaaa ggaaaccgct tcccgcaagc agccgggata tctggtcagg gcttccgtca
    19501 cagtcaatat cgaaactgaa tttattgtct gacagaattg ccgaaggagc atcgaaattt
    19561 atgacaaggg gatcctttct taacgcatcc cccgctctta tgtggatttc ttcacttgag
    19621 aaaaactccc tgctcatatt cagttggagg tctattttaa tttccctccc gtcggaagtt
    19681 cttatagttc cggttgagct gaaggacacc ttttcacttt cataatgaat ggatttgctg
    19741 tcgtagatca atccccatcc ggctcttggg ttcccgttgc cttgatttgc cggaacaccc
    19801 cggttaatgg gcatatcact gttgattctg atctttttcg gtattttgaa tctgaatttt
    19861 ttgcccagaa gctgtgtcag cattgcttcg ataagcataa gcttttgttt gtcttctccg
    19921 gcaagctcct cttcaatatc agtatcaatg cattgggaat tggtgctgct gatttgctga
    19981 tgaagttcca tggctttttt tgagatatca atgatgattc cgggagggct attcattgat
    20041 gtttcaggat tatcacctat ccagaactgc atgttttcct gtttctgttt aaaggttacc
    20101 gaggagtgct cgctctttaa acagatgttt gaattctcga tcttcatttt tccacctccg
    20161 tgtgacatta cgccaaagcc gcaatccttt tttgctttgg tatagattat tatataagtt
    20221 atcggaaaaa ttccgcatat tctttatgtg tatacatgaa tatgctacaa taattgtatc
    20281 gatacaaaaa ggggtgaggg tatgggtgaa gtgataattc cggaagatat agaaataaat
    20341 atagacaaaa gcagcggcag tccgatttat atacagctgt atgagcagct gaaaaaatat
    20401 ataattgaag gtattgttac ggaagactgc aagcttccaa caatcagaga cttcgctgag
    20461 catctgggag tgaacagtat aaccatagtc aatgcataca agctgttgga ggataacaag
    20521 ctggtttata agaaggtggg aagcggaacc tacgtaatgc cgctgaaggc tgtgataaac
    20581 cttgaaagca atttcaccta ctctttggat gaaatagagt ttgacatgga tatgggagct
    20641 gatgaggata tcataaatct ttcaacagct tccccggacc caaagctttt ccccataaaa
    20701 gacttcagga ggatgctgga tgaggtattg gaacgggacg ggggaagagc cttcatgtat
    20761 catgaaaata tggggtataa acccttaagg gagtcggtat caaaatacct gacggaatat
    20821 ggaattaacg cggaagccac cgatatacac gttatttccg gtgcacagca gggtatagat
    20881 attatatcca gagttctttt aaccaacgga gactgcgtct ttgtagagag tccgacatat
    20941 aacggggctg tagccgtatt caagtcaagg ggggcaaaga ttgttgaaat accgcttatg
    21001 gcagacggcc cggatatgaa ggaactggaa aaaaagcttg cgcttattaa gcccaagctt
    21061 atatatgtta tgccgaactt tcagaatcct accggataca cctattctga aaggaagagg
    21121 aaatatcttc tgctgctatg caaaaaatat gatgttacgg tggttgaaga tgattacatg
    21181 agcgacctga gttatcttga cgtagggaaa aggcctctta aggcgcttga cagggaaggc
    21241 agggtgatat atataaaaag cttttccaag atattcatgc cggggcttag agtgggatat
    21301 ctgataattc cggaaggctt aaaggaaaat gttatgggca tcaagcatat aacagacata
    21361 tatacctcag gattaatgca aagggttctt gatttgtatt tcagaagcgg catatggagc
    21421 aagcacttgg aatatatgaa gcgggaatac agcctgaggt attttgaagc agtaagatct
    21481 gcaagaaagt atcttagggg ggcaagcttc acccagcctt atggagggtt gaatctgtgg
    21541 gtcaagctgc cggagaaggt atcatccgtt gaactttttg aaaggtgcag agacaggaaa
    21601 gtgattttta cgccgggcac cgtattcata aagggggagg ccggagagat gcatataagg
    21661 ataagctttg ccgctgcaag cctcggaaaa atcgcggaag gaatatccat aataggaaat
    21721 gtaatggagg agattaaaga cagtgctaaa taatctcctt taagtccttt actgcatttg
    21781 catcaagctt tcctatatca tggagcaagg ccgcaatttt cattttcatt gttgattcct
    21841 tgtcaaaatt gtaatactcg caaaacattg aggcaggatt ggtattgata ttttccagta
    21901 cggctgttca tcattgtaaa taagctcaaa catatcagct atatgtatta tctgggcttc
    21961 ctcaggaatg tcacttcctg caagcccgtt ggggcctgag ccattccagt tttcgtgatg
    22021 aaattttatg aatcttgaaa tacattatta agcttttctg caatgcaaag tgatgcaagg
    22081 gctgcccgtt ttgagtggct ggcgaagttg tggttatata tgctgaatct tggtatagga
    22141 acattagaat taatcctttt gtgttcgtct ttagaaaatt atgatgtttt tttgattact
    22201 taaataactt atagggaaca cttgaagttg agaatttact taagcagaat cggaggtaaa
    22261 tgatgaagtt ctacataggt atagacgtag gatctgtgag tacaaatgta gtagcagtgg
    22321 atgacagctt caatgtatta tcaaagcaat atatcagaac aaacggccag cctcttgaat
    22381 cggtgaagga gggactgaga aaccttaaag cagagctggg cagcggctca aaggttttag
    22441 gcgcaggtgt taccggcagc ggcagacagc ttagcggcat aatggtggga gcagatgtag
    22501 taaaaaatga aataactgct catgcaactg ctgctattac atattttcct gacgcaagaa
    22561 ccatatttga aatcggcgga caggattcca agattataat gcttaagaat ggaatggtag
    22621 ttgattttgc aatgaacaca gtgtgcgctg caggtacagg ttccttcctg gaacatcagg
    22681 ccgaaaggct taaggtgaga atcgaggagt tcggagacct tgctctgaga gctgaaagga
    22741 acgtcaggat cgccggcagg tgtacagtgt ttgctgaatc cgatatgatt gccaagcagc
    22801 agtatggctt ctcaaaagcc gacattatca atggattgtg tgaagctctt gtgaggaatt
    22861 acataaacaa tcttggaaga gggaaaaagc ttgaaccgcc ttttgtattt cagggaggag
    22921 tagccgcaaa caaaggtata aaaacagcct ttgaaaaggt aatagggcat caggttgttg
    22981 ttcccgggca ttacaacgta atgggggcaa taggtgcggc aatgcttgcc agggagtata
    23041 ttttaaggag cggcaaaaca acagggttca gaggatttga ggccgatgac tttgattttg
    23101 tgcctgcaag ctttgagtgc tccgactgtg acaattcctg tgaagtaatt aaggttgaaa
    23161 tggacggcaa ggttgcggca atgtgggggg acagatgcgg caagtggact aattctgtca
    23221 ataacaatcc cttgtaggat tgccggtatc acagcgaaaa aagtatatct tgaggcacct
    23281 tttttttaaa aggttgcata tatttattcc ttccgtaata actttatata aagaaatata
    23341 actggctaaa agtaattgac taagatggga gggatattta tcaaaccgag aattatttta
    23401 ttaaccaaga aaaatctcat aatttatgct gtattattca ttgttctggt tattggcttg
    23461 gtcttattgt ttacattaag aagcaatgaa gcggggcttg ctgcttcagg ctatacatat
    23521 ttgaaataca atgatggggt atatgtaggt gcagagaaaa cggaatccgg ggatgtcaag
    23581 gcggaggtaa caataaaaaa cgcaaaaatt aaggacataa agcttatcga aatgcccccc
    23641 gactacataa gcaacaatcc caacatcaag gatgaaattt ccgacgtaat ctatacaata
    23701 ataaagaaac aggatgtggc agctgcaagt gatacgggaa ctgcctccta tgtgttgagc
    23761 aaggtaataa aggcagtaag aaatgccctt aatgaatcta tgttagtaca gtgaaaaaag
    23821 aatgggacat ggagacgggg acatgggggg agacatgggg acgtttcgcc tgtcccgctg
    23881 aaagcgggac aggcgaaacg tccccgtgtc ccccccgtgt ccccgtgttt cgtgcctcgt
    23941 atccggaaaa tcgtttagtg attgcgtttg cagctgggac aatagccgaa gaaataaagc
    24001 tgttgattgg taagctggta acccgtcttt ttctgcactt gatccgcaag gtcaaaaagc
    24061 atttcatcgt atacgtcttc aacttttcca caagctatgc aagtaatgtg aggatgttga
    24121 tttacatttg catcatatcg gaatttatct tcccctacat tgagctcttg aaccaaccca
    24181 agatccttga atacctcaag ggatttatat actgttgcca ggctcattgt gggatatacc
    24241 ggctgaagct tgctgtacag catgtcaaca cttggatgct cttttgtatt cttcagtatg
    24301 tcgtaaacag ctatacgctg gggagtaact ttatagccct tttcctttaa gaatttaatt
    24361 aaatcaatca tattcattcc tccagcgaaa ttatttttta tcaaaaagga gctaataatc
    24421 catattgata cgctatacta cctgaatgaa ccggaaactg cagcaagatg catacattac
    24481 agctatcttc attatgataa attttatcac aatgcggcat acaaaaatca taatgtaata
    24541 atttgttttt gtcaatatta attaggaaaa atttgtcaaa tttgataaat atagtacgaa
    24601 agctatcaat aataaatatt aataaatata ttatataatt taattataac actaaattgc
    24661 aaaatgtaaa taaaattatg aaaacattct tcaaacaccg gaagcctgat ttgagctgcg
    24721 ggtaaagcaa tgagtagcaa aaaaacggga atccactaaa aacacggtgg agaaaacaaa
    24781 gacatccgtg tattctgtgt tacccgccct ttgtaacctc agggccctgc ctattctaat
    24841 cctgcggcta tcttgactgc tttctggtgc cccggatctg atctgcttcc gctgtgagtc
    24901 ctgctgttcc tgaagtgtat gcagaagtgc ccgttcattc cattatcggt tatatcctct
    24961 cccccgtggg gcattccgtt catggaggct gcaatttttc tcagaacgcc ctcatcatct
    25021 tctatagtta atattatagg ccttctgttc caactccagc ttccgccgta tatgctcttc
    25081 atgatttttg tatcctcagg ggtcagaggt tcggtatcag cgtggtttct gccgtacaat
    25141 ctccggacat taaaggattt gcctgtctca acatcggtga ctgtggcttt gtcaccctta
    25201 tcgtatatgt actgaacatc tttgaaccaa tcaaggtatt cgccctttgt gccggaggca
    25261 tcggaagctt ccgtagtatc ttcttcaaca tcttgatcgg ttccttcagg aatttttatt
    25321 tcctgtccaa tctgaataac ggtatcatta ttaaaattgt tttcattctt tattgactct
    25381 gcagaaacac cataatcctc cgctatggat tctattgtat ctccttcctt taccacatat
    25441 atatttttgt tatgctcata tatattgcca agctccgaac tgtcctgcac ggagggcgga
    25501 ggtaccgtct gcacaggggc aggcttcact acttttacaa cattgggaat tattatttca
    25561 tcacctatgg taagtatgct tttttcatta agtccgttat aatcaaggat ggattctacg
    25621 gagacattat agtcacccgc aatatcccat atggtatccc ccttttttat tttataccgg
    25681 acattaacct ttgtagtgtc ttttttggcg gaggaggaac ggcttgggat tgcaatatgc
    25741 cctcccgtca gctcattaag ctttccgatg gttatggggc ctattattcc atcggtgcct
    25801 aagttatatt tttcttgaaa tttcaatact gcgccttcgg taacttctcc gaagtagcct
    25861 gtgcagacct cgtcaaaaaa gccgagcttt ttaagtgcag cctgaatctc tgcaatttcc
    25921 gccgaagaat ctcctctttt gtatacggat gtgtcggcat aagacggaat aaccgtaata
    25981 aaaagtatga tcagggttag catagctaat gctattcttt tgtaagcaga ttttttcaat
    26041 tggatatctc ctctcaaaag gcttacgggt tagcattcag cctgtctaat tagtttcatt
    26101 atactacagt tatgcacttg tgaaaagcaa atttttccaa gagcaattgt gccgtaatgt
    26161 taaatttagt attagcagga aatatataat atgagtcgaa ttataatatc ataatatata
    26221 aggatttatg attgtgctat gcgtgttttc gatacgccga aaaatccggg aagatgttcg
    26281 ggggaaaaaa gagaaaaggc aaatagcctg atgaataagc cagggaggag atgcttatgg
    26341 aggggcaacc ccacagttca tatgacgctt atctcttcag ggaggggaac cactatagga
    26401 gttatggttt tttgggtgca catttcacta atattaacgg tactgacgga gcgcaattcc
    26461 ttgtatgggc tcccaatgcc gatgctgtgc atctgatagg ggattttaac aattggagaa
    26521 gcggcgccaa tcccatggta aaggacgatg attccggaat atggtcagta tttgttcccg
    26581 gcttaagaga gtgggatatg tacaaatatg agatgcactc cagggatgga agagttaatg
    26641 caaaatcaga cccctttgcg ttttattcag agcttcggcc gggcagcgca tcaaagcttg
    26701 tttcccttgg aagctataaa tggaatgacg gcaattggat ggagaataga aaaagaacaa
    26761 atatttatga aagccctgta aacatttatg aggtacatgc aggctcatgg aggcaaaaac
    26821 ccgatggtag gtattattcc tatagggaac tcgcggaaga actggtggac tatgtaaaga
    26881 atatgggctt ttctcatatt gagatgatgc cccttacaga gcatcctttt gacggttcct
    26941 gggggtatca gtccacggga tactactcag tcaacagcag gtatggctcg ccttatgatt
    27001 tcatgtactt tgtagacaaa tgccatcagg agggcatcgg ggtgatactg gactgggtac
    27061 cggggcattt ctgcaaggat gatcatggcc tgaggctttt tgacggtaca accctttatg
    27121 agtacgaaga tcccaaaaaa ggcgaaaatt acggatgggg aacctgcaat tttgatcttg
    27181 ggaagcccga agtgcatagc tttttaatat caaatgcggt gttctggttt gacatatatc
    27241 atgtagacgg cctgagagtt gatgcagttg caagcatgct gtaccttgat tatgacaggg
    27301 aaccgggaga atggataccc aatattttcg ggggcaggga aaatcttgag gccgtggatt
    27361 ttgtcaggaa gcttaataaa gttgtttttg agtattttcc cggtgtaatg atgatagccg
    27421 aagagtccac cgcctggccc cttgtgacgg cccccgccta tctgggcgga ctgggcttca
    27481 actataaatg gaatatgggc tggatgaacg atatgcttaa atacatgaaa acagatcctt
    27541 cggacaggaa gcggcaccac aaccttctca ccttttctct tatgtatgca tattcagaga
    27601 attatgttct tccgctgtcc catgacgagg ttgtccatgg gaagaagtcc ttgcttgata
    27661 aaatgcccgg ggactattgg caaaaatttg caaaccttag agtttttctg gggtatatgg
    27721 cagcgcatcc ggggaagaag ctgcttttca tgggagggga gctgggacag tttatagagt
    27781 ggaggtatga ccatcccttg gattggatgc tgctggatta tgaaatgcac agggggatac
    27841 agaactatgt aaaaatgctt aatcaggtat atgcaaggga accggccctt tgggaactgg
    27901 atcatgtatt tgaagggttt gaatggattg atgcggataa ttcgatgcaa agcataataa
    27961 ccttcctgcg taaggggaaa aggccggaag ataccgttgt tgcagtatgc aacttcactc
    28021 ccgcagtata cgagggctat aaaatcggag tgccgtacaa ctgcagctat tatgagatat
    28081 tcaacagtga ccgtcaggag ttcggaggtt caggcttcaa tagtacctta atattttcag
    28141 ctgttaagaa ggaatggcac aaccggcagc agatgatcaa aattaatgta ccgcctttgg
    28201 cagtattgta tttaaaacct gcagccattc cggagaatga aagaaaaatg gaaacagtac
    28261 agctggaaac cgcagcggca aaataataga cctgtttttt agaataaatc atcgggggct
    28321 ttaataaata tggatagaca tattaattag aggaggggta cgcatgaaga aaaaggaatg
    28381 gatagcaatg atcctggctg gcgggcaggg aagcaggctt acattgctta ccaaaaagat
    28441 tgcaaagccc gctgtgtact tcggaggaaa atacagaatc attgattttg tattgagtaa
    28501 ttgttccaac tcaggaattg atactgtagg tgtattgaca cagtatcagc cccttgtatt
    28561 gaattcgtat atagggatag ggagcccttg ggacctggat agaaagaaag gcggagtcac
    28621 actactgcct ccttatgtga atgaaagggg gggagaatgg tataaaggca ctgcaaatgc
    28681 aatatatcag aatattaatt ttgttgatga gtatgatccg gattatgttg tagtactatc
    28741 gggagaccac atctacaaaa tggattactc aaagatgctg gactatcata aggaaacatc
    28801 ggcagaagca actattgcag taattgaagt cccatgggag gagacatgca gattcgggat
    28861 tatgaatacc gggcctgacg gcaggataga agagtttgcc gagaaaccaa gaaattcaaa
    28921 aagcaacctt gcttcaatgg gtatatatat attcaactgg gatatcctga aaaaatacct
    28981 tgtagaggat gaagcagatc cggaatccag caatgatttc ggcaaaaaca tcatacccaa
    29041 gatgctgaac agcggaataa ggctttttgc atatcccttt gacgggtact ggaaggatgt
    29101 cgggactatt gaaagcttgt gggaagctaa tatggatctt ctgaaggagg ataacaggct
    29161 taatcttttt gaccccaaat ggaagatata ctcggttaat gcaataaagc cgcctcattt
    29221 tgcgggagca acagctgaca taaagtgctc catggtaggc gaaggctgtg tcatacacgg
    29281 ggaagtcagg aattccgtcc tgttcccggg ggtctacatc ggcaaggaca caaaagttat
    29341 agacactgtg gtgatgccca aggttatgat tggtgaaaac gtagtagtag aacgtgccat
    29401 aatcggtttg gaatcagtta ttaataacgg ctgcaggatc ggtgacagta catcaggatc
    29461 cataacactt ataggagaat atgagcagct gccccaagga acggtatata tctaaggatt
    29521 cggtagaata aactgaagga ggggatacta tgctggatta tatgggaatc atcaacctga
    29581 atgagagaga ggacaatata aaaggattga caggccatag acctttagca gcggtaccga
    29641 tagcaggaag atacaggatg atagacttcg ctctttcaaa cctggtaaat gccggagtcc
    29701 agaatataag catatttacg cagaacaagt acagatccct tttggatcac ttggggacag
    29761 ggaaagcatg ggatctggac agaaaaaatg acggactgtt cataatgaat ccaatgtatg
    29821 attattacaa cttgggtatt tacaggggcg atatcgagaa tttcaaatgc catatggact
    29881 atttaaattt cagcaaacaa aataatgtga taataacccc cagcaacatg atatgcaacc
    29941 tggattacgg ggaagcaata aagcagcaca atgaaaccgg ggcggatata acaattatct
    30001 ataagaaagc agacaattgc actgtggatt tccagtactg caacacactt gaaatggatg
    30061 aatacggaag ggttgagcgt attgatataa actttggaaa tgaaaatgaa tgcaatgtat
    30121 ccatggatat gtacataatg aaaaaatcac tgtttcttga tattgtgaac agctgtgttt
    30181 caacaggcta ctgcgattac ttcagggaag cagtgtataa gtacctgcat aagctgaaga
    30241 tattcggata cggatttaaa ggctatctgg cctgcattaa ttcagcttac agctattaca
    30301 agacgagtat gcagcttttg aatatggata tttccaagga gttgtttttt aagaatggcc
    30361 tcatatatac aaaagtaaag gatgagcctc ctacaaaata taaagagaat gcgagggtaa
    30421 taaattcact tgtagcaaat ggatgcacaa tagacggcca ggtggagaac agtattctgt
    30481 tccgaagcgt aaagattaaa aaaggagcag tagtaaaaga cagtattatt atgcagaatt
    30541 gttcgataga agaggagtca agggttataa atgccatatt ggataaaagc gtaagggttt
    30601 caaagggtaa gcggataata ggatatgcgg acaatcctga gataattgag aaaaaagcgg
    30661 taatctgaag acatgaataa cacttaagca catccccgcc gaagcatcgg cagggatgcg
    30721 ctgtattgtc tataagcttt ctaaaaattg tacccgaaag ccgtcagggt cagcaacaaa
    30781 aaagaagcgc acgtgttggt tcggctgtat tatatcactg acaacagaaa tatcggtacc
    30841 tttaaagctt tcaaacttct cgaggatgtc ctccacttca aatccgacag aaacagcatt
    30901 gccgacaatt gcatcaggtt ggttcctatg gcagataagc tctacttttg tttctccggt
    30961 tcccaggaag gctatctcgg tatcgagtcc cggctgatag cgtctgtcaa cgggtaatcc
    31021 tacaatttca gtgtaaaaat gtatggatgc atccaaatct ttgaccgtca gtgtaacatg
    31081 gcgaaaattc atgaactgtt cctcctgtct tttttatcgg tatgatgcgg tatcatttct
    31141 taaattataa acaattattc atgcgactgt caaagtgcaa caaagctgtg cctttttcat
    31201 atcttgttat taaattgcaa ttattatcct tggttaatgt tgtaaagata ttgtataata
    31261 gatgaaataa ttaattttta tctgaatttt atgttatgag gtgcaaatat gtctgtcgat
    31321 aagaataaat tcaaggaaga ttatttaaaa aagcttatga ccatgcatgc aaaggctttt
    31381 aaagaggcaa ccaacaatga aaggtattca gctctgggaa gccttataag ggattatgcc
    31441 gcaatggatt gggtgagaac caacgaagaa taccgcagaa agaatgcaaa gcaggtatat
    31501 tatttttcaa ttgaattcct gctgggaaag cttctgggca acaacctggt gaatatggga
    31561 ctggaaaagg tgtgtgagga agtgcttcaa gagctgggct gcaccctgaa ggaggtaaaa
    31621 tctgccgagc ctgacgcggg gctgggtaac ggaggcttgg gaaggcttgc cgcttgtttc
    31681 ctggactcca tggcttcact tgggataccg gggcacggct gcggaataag atacaagtac
    31741 ggtctttttc agcagaagat tgttgacggc taccaggtgg agctgcctga gaattggctc
    31801 agggaaggga atgtctggga gatgaggaag gcgggaaagg ctgtggaagt aaggttcgga
    31861 ggcaaggttg cccccgagta taagaataat aggctggagt ttaagcatgt ggattatgag
    31921 acagtacttg ccgttcctta tgacactcct ataatcggat ataataatgg gacagtcaat
    31981 accctaaggc tttggagcgc ggagaatccc gaaaaggatt ttgatttgtc ctccttcagc
    32041 aagggagatt atatgaaggc ggtagagcac aaatattccg tggaatctat atctcaggtt
    32101 ttgtatccgg atgacagctt cgccgagggc aggaagctca ggcttaaaca gcagtacttt
    32161 ttcgtatcgg ccggccttca gagcattgta aggtcatata aacgtactaa tgacaatatt
    32221 tatgattttg atgagaaggt ggcaattcac attaatgata cgcatccggc cattgccatt
    32281 cctgagctta tgaggatcct catggatgag gaaggtgtcg attgggatac ggcgtggaag
    32341 attactacca atacaatttc ctacacaaat cacaccattc tgtccgaagc cctggaaaaa
    32401 tggcagatag acatgtttaa gcccatgctg ccgaggattt acatgattat tgaggaaata
    32461 aacgagaggt tctgcgggga tctttggaac agatatcccg ggcaatggga caagatatcg
    32521 agaatggcca ttctggctga tgggaatgtg aaaatggccc accttgcaat agtgggaagc
    32581 cacagtgtca acggtgttgc caagattcac actgaaatat taaaaaaaca ggagcttgcg
    32641 gacttttacc acttttatcc ttataaattc aacaacaaaa ccaacggaat cacccacaga
    32701 aggtggctct taaaatgcaa tccggagctt gcgcagcttc tcacggataa aatcggaggc
    32761 tcctggaaaa agcatcccac agaccttata agattcctga agtatcggaa tgacccggag
    32821 gtgcaggaaa gagtaagaca gataaagcag aacaacaagc ttgagctgac aaaaattatc
    32881 aaggacgaat actccattga aatagatgta aactcaatat ttgatataca gataaaaagg
    32941 atgcatgcat acaagcggca gctgctgaat gcgcttcata taatgtacct gtacaattgc
    33001 ctgagggaga atcccaggct tgatattata ccgagaacct tcatattcgg cgccaaagct
    33061 gcaccgggct acaagctggc aaagcaggag atcaagctta tcaataccat tgccgataag
    33121 ataaacaatg accaaagcat agaaaataag ctcaaggttg tatttatgga ggactacaag
    33181 gtatctctgg cggaaaagat aatacctgct gccgatataa gcgagcagat ttctactaca
    33241 acgaaagaag cctccggtac gggaaatatg aagcttatga tgaacggcgc aattactgtt
    33301 gctactttgg acggagcaaa tatcgagata agggatgaag taggggacga aaacataata
    33361 attttcggaa tgactgagaa ggaagttttg gactattaca ggaacggcgg gtacagcgca
    33421 attgagattt acaacagcga tccgcgcatc aggactgttg cagaccagct tataaacggc
    33481 tttttcccgg tgagcagcgt tgagtttctg gatatctaca atcatcttct tttgcataac
    33541 gatgaatact tcgtgctgaa ggacttcaat gcttatgcgg aagcccataa aagggtggat
    33601 gaaacctaca ggaataaatc cgaatggatg aagatgagta ttgcaaatat tgctcattct
    33661 gggatttttt ccagcgacag gacaatatcc gaatatgcct cgggcatatg ggaggtcaaa
    33721 agggtgatac tatgacggat atgatgattc tgcacaactc ccgccgggag aaatatcgct
    33781 ttccctttgg tgcagttccc tgcaaaagca cggttcgcct cggcattact gtgaaggcct
    33841 cgcagccggt gaattcagct gaagttatag taagcacaga aggcggcatt gaacaaagga
    33901 tagccctaag tgcgccggaa gggcagggag aggactatta tgcaaatatt gaggcacctg
    33961 agaagccggg gcttttatgg tattattttg aactggttat aggggataaa aagtattatt
    34021 acggaaataa cgaaagattt ctggggggca ttggaggtgt atacggtaca attccgcccc
    34081 ggtatcagat taccgtttac agaagagaag caagtactcc ttattggtac aaaaaggcca
    34141 taatgtatca gatatttgtg gataggttca gaaacggccg tgaagactgc aaggtgctga
    34201 accccaagaa aaacagcttt atttatgcag actggggcga tactcctttc tatataaagg
    34261 actgtgagaa gggaagtatt gccaggtggg atttttttgg cgggaatctt cagggagtga
    34321 cagaaaagct ggattatctg aaggagcttg gtgtgtctgt tatatatctg aaccctgttt
    34381 ttgaggcacc cagcaatcac aagtatgaca ccgccgatta caagaatatc gaccctatgt
    34441 acggcagcag cgagaccttc agggaccttt gcgaaaaggc aggaaggctt ggaatttccg
    34501 ttattttgga cggggttttc agccataccg gcagtgacag catttatttc aacaggtacg
    34561 gcagctatcc ggacatcggg gcatatcagt cagccgaatc tccatattat tcatggtacc
    34621 gcttcaattg ctccaaggat gattatgagt gctggtgggg tgtggacagc cttcccaacg
    34681 tcaatgaaat ggatccgggg tatatcgact ttattatcac aggtgaggac agtgtcatca
    34741 agcattggat gaacctgggg gcaaagggct ggcgcctgga tgtggcggat gagcttccgg
    34801 atgagtttat ccgattgatt cataaaacaa tgaaagagaa agataaggat tcggtgcttc
    34861 tgggggaagt atgggaggac gcatccaaca aggtgagcta cggaaagaga agggcatacc
    34921 ttttggggga ggaattggat tctgttatga attacccctt cagagggata atgatagatt
    34981 tttttaccgg gaagcgggat gcagaatatg taaatgaagt gctcatgagc atctatgaga
    35041 actacccttt gcataacttt tactgcaata tgaatttaat aggctctcat gacgttccga
    35101 gaatactcac aatactgggg gaagcgcctc cggaaaagca attgacacaa tctcaaaaag
    35161 aaagctacag actgtcggaa agccaaaaag ctttgggaat agcgaggctt aagcttctgg
    35221 ctcttattca gatgactttc ccgggggttc cgggcattta ttacggcgat gaagcaggac
    35281 tggaggggta cagtgaccct ttaaacaggg gaacctatcc atggggcggt gaaaaccgcg
    35341 aactgcttga atggtataaa gccataacct ctatcaggaa caagtatgat gttttcagca
    35401 ccggaagatg gattcccata tattccgaag gggatgtata cggatatgtc agaaggacgg
    35461 aaaagggcag ggatgtattc ggcagactgt gtgaggacaa taccgcactg gtgctggtaa
    35521 acagaagcag ggacttccgg tatcctctgt ccctggatat tagtcaatgg cacaggggtg
    35581 tcatttatga cattcttgaa ggcggcaagg ctgcagatat tggagaagga atactggatg
    35641 ttaccctgga accccttcag gggaaggtat acattgcgaa actatagcga acaagaggtt
    35701 tgattgcatg agcataaagg aatcaatact cctgaggagc aacagcatac aaacaaaaaa
    35761 aagccgaaaa gacagaaagg acaagggagt gttctatact cccccgcagg tagtggagag
    35821 aattaccgag aagcttctgt cgggggttga cctttcggca aacccttata taaaaattat
    35881 ggatcccgcc tgcggcacag ggcttttcct tataaaggcc tttgaggtgc tgaagagaaa
    35941 gtttgaggaa aactacgagg ttgtattgga caggaatgag gaactgagag gtgtgctgaa
    36001 aaaggacgga atcggcagct tcattttgga aaacaacctt tggggtgccg atatcgaccg
    36061 ggaagccttg gatgcagcgg ctgaaatgct gatgaagctg gcaggcaggg agtccaaggt
    36121 gaatctggtg tgctgcgaca gccttataag cggaagtacg gctcaaatga gcttttttga
    36181 tgatataccc ccggaggaat accaattatg gaacagcagt tatgattaca taatagggaa
    36241 ccctccctac ataggacata agcaggtatc gggggagtat aagcaagtgc ttcagcagct
    36301 gtataaaggc atatacaagg ataagtccga tatttcctat tgcttcataa aaaagggcat
    36361 agacctgtta aaggatgggg gaagcttaag cttcgttact tccagatact tcatggaagg
    36421 gccttcggca gccgggttga gggaattcat tgccggccat tgtacagtta cagaggtgat
    36481 ggatttctat ggggataagg ttttccgcga tgcgggggtt gcagcatgta ttatcaccct
    36541 gaggaaggga atatttgatg gggagacttc agtcctgaag cgaaggcagg gtataaaggg
    36601 caatgagcct gaattgtttg tcccgtccga ctttgagcat tttacggtca aaagggcgga
    36661 attaaaggat gagggctggg tgctgctgag ccctgaaaaa tatgaagtat tttctatggc
    36721 agaagcgggg gggagcctga aactggaaga gattgcagac agttaccagg gtattatcac
    36781 cggctgtgac aaggcattca tccttaacag gagtgagata gaagagaata agatagagga
    36841 agtactgtta aagccgtgga ttaagaacag caatattcag aagcattgta tcaagcctgc
    36901 tgataagttt cttatttatt cggattttat taaggatgaa aaggattttc ccaatgccgt
    36961 aagcttcatt tcaaggtgtc gggacagact gctgaacaga agggaatgca ggaaggggat
    37021 aaggaagtgg tatcagctgc agtggggcag gaccaatgag gttttcgaca cacccaaaat
    37081 agtatatcct tttaaatctg caagcagccg ttttgccgtt gatgaatccg gcaattactg
    37141 cagtgccgat gtatacagcc ttaaactaaa aaaggaatat gaaggaagga taaccttgga
    37201 atatattaca gccgtactta attcaaggct cttggaattt tatttcaagt gttatgcaaa
    37261 aaaagtcagt aaagatttct atgattatta tccgaatacc gttctcagga tgaaaatccc
    37321 catgccggca gcggtcaatc ctatagggga attggcatca aagctcaaaa attgtaagaa
    37381 tgaggatgca agaaaggctg ttacttatga aattgatagg gaaatatata ggttatatgg
    37441 attaagcaaa aaacatataa aaattataga ggaagatagc atgatatatg agtaatgcga
    37501 cgtgtctgag ggacacgcga aacgtcccca tgtccccggg agaaacgtcc ccatgtcccc
    37561 gggagaaacg tccccatgtc cccgcgtcgc attattcttc taaggaggag ttttatgctt
    37621 ttgctggctg ccgccgttgc cccgtccgcc gcattattat actactttta tacaagggat
    37681 aaatatgaga aggaacccag aaaaatgctt gtgaaagcgt ttctgatagg cggagggctc
    37741 gttgttccgg tactttttat agaattgtca ctgaatatat ttgatatagc ggaggccaac
    37801 cttcttgccg ccggttatac tgcttttgtg gtggcggggc ttgtggagga atcttccaaa
    37861 tttctgttct ttatcttata catatggaaa catagggatt tcaacgaaat gtacgatgga
    37921 atagtatact ccgtgttcat ttcccttggc tttgctaccg tcgaaaacct tgcatatgtg
    37981 ctttccaccg gttttagtac ggcagttgtg agatctctga ctgccgtacc ggctcatgcc
    38041 ctgtttgccg tagtaatggg atattacctg ggcattgcca ggtttgcaaa gacccggtac
    38101 agacggaagt atatcatact gggacttatt attcccgtat tgcttcatgg gatatatgat
    38161 tttatattgc tttctcagaa gttgtatttg ctggtgcttt tcataccgta catgctgtat
    38221 ctctggaaaa gaagcctcgg gcatgtggat gaactggtgg agtcctcacg gtttaaggat
    38281 aggccggaag aatatgacga tgacactgaa ggccgtgtct agtgtctagt gtctcgtgtc
    38341 tcttgcctct tgcctggcat ttacatagtc cacaaaagcc tccagtctgg tttggaagcc
    38401 tgcttcccct gtctgctcat caacaataag gtacagtatg ggtatattat aatccttttc
    38461 cacttgtgaa agaatcgaac gagatactat ttccggcata caggtcatgg gataaatttg
    38521 tacaagcccg tcatagcctg tttttgcata gtgtaccgca tgaccgatgc attgctgggt
    38581 atgccctcct ataagagcag gcaggtaggg ctttgacaat ttcttttcca gcttatatcc
    38641 cttccttccc gtggcgggaa atatgacatt attgcgtgcc caatccctta ccgtcattga
    38701 tctgtgtacc tctacgccca gatctcccag cctttcctcc gtatggagat tagaaaaagg
    38761 ctcgattata gtatatattt ctccgataat tcctattttt aaaggcttga agcttttgtc
    38821 aatatcaatt cctcttattc ggtttttggt gtcatgtatc aactccagca tttggcctga
    38881 tccctttacg gccatcacat cgctcttgaa cttatgcatg atgcttcgca attcatcttt
    38941 cttgaaggct cgcggcctgg tataataaag caattcattc atactgtcca attcgcagca
    39001 taccttgtat gcattgataa aagacggtat gattcttccg ctgttttttc ccgcaacttt
    39061 ttttaatgct tcaagaaatt tccgataccc ttccctgggg gggtcaagta ttataatatc
    39121 tgtatcttta taacctgcct cccataatgc ttgcttctgc aaggggccat agtagccaaa
    39181 cctgcaaggc ccgcagcttc cggttataag aatggtatca gctccggcat tgatgctgtt
    39241 tatgtaattt ccgagcatta tcttgtacgg caggcatact gcctcaggtg ataaagaaga
    39301 gccaagcctc aaggtggaat tggtagtgaa tgccggtatg atgctttcca ctcctatatc
    39361 gtccataaga ctcttaactg ctatatatgc ttctcccatg tgcggatatg taagcctcat
    39421 ttgtattcct ccaatccaac aaatcaaaaa aagcctctat ccttgtgtta aagctggcat
    39481 cccctgtatg ctcatcaata ttcaacacca tgtacggttt tttataatat cttcgtgcct
    39541 gcttttctat
//
LOCUS       DAOQUR010000019        39029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1208_length_39029_cov_11.556217, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000019 DAOQUR010000000
VERSION     DAOQUR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 39029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39029
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_39029_cov_11.556217"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..370
                     /locus_tag="PKI50_05845"
     CDS             245..370
                     /locus_tag="PKI50_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03269.1"
                     /translation="MAFISSVMVIVRWFKGKKKKESDSGITEESNEQICDNNESA"
     gene            complement(393..797)
                     /locus_tag="PKI50_05850"
     CDS             complement(393..797)
                     /locus_tag="PKI50_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNT03270.1"
                     /translation="MMSKKNYKMIGILICIAAIGFGVFYFINIAPANRVLYEVRNAEI
                     KDIDLNEIADGKYTGEFSYNNTRCMVEVIVNNHRIEAINVLECGTPEYGKKAEAVIDR
                     IIDKQKTNVDAVSGATTTSKALLKAVEAALSK"
     gene            1016..2218
                     /locus_tag="PKI50_05855"
     CDS             1016..2218
                     /locus_tag="PKI50_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03271.1"
                     /translation="MIQRKEYLNKLIEWREKQVIKVVTGVRRCGKSTLFSLYIDYLKK
                     TGVSEEQIILINLEDIEYENLLDYRPLYDYIKARIVKGKYTYVFIDEVQQCKSFEKAV
                     DSLFLKENVDVYITGSNATMLSGELATLLSGRYIEISMLPLSFSEYLEFAKGRDVKST
                     FDEYLKFGSFPYVAVLNKSDAVVRTYIDGIYNTILIKDVAARERIKDISVLKSIVKFL
                     CSNIGSPVSVKKISDAINASGRKISVNTVENYLRALYQSYIFYKVDRYDIKGKQQLKT
                     LGKYYIVDTGLRNLLLSSSAADIGHLLENVIYLELIRRGYKVNIGKLAEKEIDFVAGN
                     TEGTEYYQVSASVLDRNTLQRELEPLQKIPDHHPKFLLTLDDIMPNANHDGIRQINAI
                     DWLIKKKP"
     gene            complement(2249..2325)
                     /locus_tag="PKI50_05860"
     tRNA            complement(2249..2325)
                     /locus_tag="PKI50_05860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2289..2291),aa:Arg,seq:acg)
     gene            complement(2397..2585)
                     /locus_tag="PKI50_05865"
     CDS             complement(2397..2585)
                     /locus_tag="PKI50_05865"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HNT03272.1"
                     /translation="MQAPTVQLGKPDKAENFDLYSVDGVDAYIKRGTRVRNDQIHIFL
                     RKFLWIKDLAVDGVRVDY"
     gene            complement(2806..3501)
                     /locus_tag="PKI50_05870"
     CDS             complement(2806..3501)
                     /locus_tag="PKI50_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HNT03273.1"
                     /translation="MNCKYHKESEAKFICDKCKQPICEECSVDVNGNKICSSCIQKSL
                     FSENRQYQKPGFFENLAFFCFAVVPGAAQMYMGLFKRGFQLMFGFISAIVLFSYLNTE
                     GMIMLIIIPTWFFSFFDSYAMRRRIKKGEEVEDSVIFDYNILIENKRIVGMVMLFLGI
                     LGVVNAFGRSVFQLVFGGDLYWSIRRSIMPIALIGAGVYMLSKSKKKEEAKNTEDIIE
                     IEEESEEEIEAAE"
     gene            complement(3503..4006)
                     /locus_tag="PKI50_05875"
     CDS             complement(3503..4006)
                     /locus_tag="PKI50_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT03274.1"
                     /translation="MCCDENRGMLHEYAAGKLSELESYAVARHLKSCDRCRRELAEIK
                     ALKVFLKSGFQESIIPSVDLKSSIMASVDLKRYKKSYRNTLGEMANLGMSLVAAGLIL
                     LLLNTVPKDDLVRVQNELYNTGENITQKISQPFSSINERIDKLTNSITQLDGIMGRIE
                     REKEGGK"
     gene            complement(3993..4580)
                     /locus_tag="PKI50_05880"
     CDS             complement(3993..4580)
                     /locus_tag="PKI50_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT03275.1"
                     /translation="MRDEEVVSYIVKSKTELFSVIIDRYQSKVFSTAFHYTHDYEEAR
                     DLTQEIFIKLYNNLQKYKGKASFSTWLYRIAVNRCIDWARKKKPQTVSAICDGSEEEL
                     DIYDTIADSGGGPEEDLIKQENAVYLRKLVESLPEIYKTVIILYYFEDFSPQEISEIT
                     GVPKRTIDTRLFRGKNLLKLKMEELICGGEQNVLR"
     gene            complement(4679..5335)
                     /locus_tag="PKI50_05885"
     CDS             complement(4679..5335)
                     /locus_tag="PKI50_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNT03276.1"
                     /translation="MYKDIKAVIFDVDGTLIDSMWIWKQVDIEFLGKRGIPLPERLQM
                     EIEGMSYSETAVYFKERFNLPESLEEIKEEWRLMAEDYYKCHIQLKSGAKEFLKLLYD
                     KGLTLGIATSNSRELVDCMLANHGIRKYFSNIRTSCEVDKGKPYPDVYLKVAEDMAID
                     PCRCLVFEDTLSGVMAAKSAGMRVFAMADEISLESKDKLIELAEGYIHSFNEVMEYFA
                     "
     gene            complement(5509..6804)
                     /locus_tag="PKI50_05890"
     CDS             complement(5509..6804)
                     /locus_tag="PKI50_05890"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNT03277.1"
                     /translation="MIRRKVGSITLAVGLITVGALLFAQNFMDIPVKDIYKYWPVLLI
                     GLGLEMILYMVLYGRNKTEVKLSVDGLCIVFIIILGIISNGIRFIDINIPGRHFFGFN
                     GSSMFEGFKYRTEISEKYSKDNISGGFNVKTLKVTNDFGEIKILPAGGNTIRLEAEVM
                     VKCNDENKAREYARNAVEIIEGEVTELSPKIPPNWNKNDHARAQVDFVVYVPKEISLI
                     ARGSFGDIEAEGIGGECRIENKNGEITASNIGGDVDIDNSFGDIEVSNIGGKADISNH
                     NGEISADNIGGSAVIENHFGDIEAEKVGGDLKVVNNNGRIYVKEVGGKADLVNAFGDI
                     GIKRADGGITVENKNGRIEAEDVAGNAQIKNAFGDIYYKSASADNGDIYARTKFGSIN
                     CNKPLQIKKEGQDTVAQGRLGTGQYKIELETNNGSIVIE"
     assembly_gap    7168..7267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7268..8499)
                     /locus_tag="PKI50_05895"
     CDS             complement(<7268..8499)
                     /locus_tag="PKI50_05895"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNT03278.1"
                     /translation="MISRVKRKSTIFIITALFILIAGGCSVKQESPNIPVPAVVPSPT
                     AKGTDVNQYIIDAVLDTANKELTAEQQVIYVNNDDVELSELYFHIYANAFRKQETAPF
                     LFDDFSMAYSRGFKPGYTEIEAIEFSKGQNRNALEYSLQGVGETILRIKLPEPLKPEG
                     SVNLRFKYKVIIPPAGERFGYGDANINMGNWYPVAAVFDDEGWNLDKYYSIGDPFYSD
                     VSDYIVSIKAPEEYIIAASGSLAEERIEGGYKNWKFHGANMRDFAFVASNNFRVAEDK
                     VGNTVVKSYYYKDHEKRGKEALDIGKRSIEVFNSAYGEYPYPTYSIVETEFPSGMEYP
                     GLVYISTKYYDNDSGADIFMYTTVHETAHQWWYGVVGNDQIDEAWLDEGFATYSEGVF
                     TEMEYGEGNGDLYYKYLE"
     gene            complement(8528..8797)
                     /locus_tag="PKI50_05900"
     CDS             complement(8528..8797)
                     /locus_tag="PKI50_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03279.1"
                     /translation="MDVILNALQSVLSMAVLTMLGYFLTLVIFIPSLMTKVFVIQSAM
                     PVIAQSAIVAAAFGGDQYYATYMVTITSILSVVFIPAYMVLLNGV"
     gene            complement(8827..10155)
                     /locus_tag="PKI50_05905"
     CDS             complement(8827..10155)
                     /locus_tag="PKI50_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNT03280.1"
                     /translation="MDVAVALIVTFMLLMLSVYRGINILYPLLIGLIIFITVAYKRGY
                     KPKEIIGMILKGIRKSVLIISIMMLIGAIMSVWRASGTVAYVVYYGIRFVDPNLFILY
                     AFLLSCAVSFLLGTSFGTVGTIGTILMVMVKGGDININIVAGAIMSGIYFGDRCSPMS
                     SSANLVSVLTETGLYTNIKNMFRTTAVPFLIASIIYGVLSFQNPLQLGENRITTGIVQ
                     TYNLNWVVSMPAIIILVLAFFKVDIKISMAVSILCGAAVAVFFQKETIGDVLKYIIMG
                     YSRPEQSFLGDIMAGGGIISMINVVLIVLIAFSLSGVLEGASLLKFAGNLIIKLGKKA
                     GIFAAVILTSIITGAFGGSQTLAVMLTYQLVRNMYDSAGIDKYELAVDLENTAIVMAP
                     LIPWNIAGAVPAAALTADNSYVLYSFYLYLIPLANLVMKKFKIIKRHKMA"
     gene            complement(10254..10937)
                     /locus_tag="PKI50_05910"
     CDS             complement(10254..10937)
                     /locus_tag="PKI50_05910"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNT03281.1"
                     /translation="MIISEVEQMKKVILDTVKESGTDLSEIDVIVGRGGNMKPVQGGV
                     YEINEAMVEDLRLGVMGQHASNLGGIIAYFIAAELGIHSYVVDPVVVDELEEYARISG
                     IPDIERKSKDHPLNQKAAARMAAKELGGNYTDYNFVVAHLGGGISVGAHKKGRMVDVN
                     NTLDGDGPFSPERSGGIAHDTEFVSWIKERMAFIAPVKVYPGENEMKALVQGVSRVLK
                     GEEQLKSYN"
     gene            complement(10987..12633)
                     /gene="abc-f"
                     /locus_tag="PKI50_05915"
     CDS             complement(10987..12633)
                     /gene="abc-f"
                     /locus_tag="PKI50_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HNT03282.1"
                     /translation="MLLLEINGLKKYYVERLILDIKDFKAYYGERIGIVGANGAGKTT
                     LLDIIAGRNIPEEGSIKLYGEISYITQLDLEAASEVDNRMAKEFGLNFNSLDTASGGE
                     KTRFKIAGSLSENNSILLADEPASNLDIQGIELLEKKLASYKGLILIVSHDRELLDKL
                     CSKIVEIEGGELKQYSGNYSQYKLQKEMEFKRAQFEYDQYIDSKRALEETVKEKKNRA
                     ASMKKAPSRMGNSEARLHKTGNQKAKANLDKAIKAMETRIRKLEKKEKPKDQQHAIID
                     IQGAEKPVSRVLISGSGVSKSFGSKELFREAEFEVLNGSKTALLGGNGTGKSTLLNMI
                     MGGDESIKLANGVKLGYFSQGTDILKDDETILENVMKESIYPEHFVRTILARLLFRRD
                     DVYKKAGKLSGGERVRTCFAKIFCSDANVIILDEPTNYLDISSMEAVENAMRLHEGTI
                     FFASHDRKLISSTADRIIYIEGRRLNTFEGSYGEYVEFRRRAADSGETERKRQKLLLD
                     TRLSEILSRLSMPGKDDDAEQLDMEYKRVLAEIRQLKSGR"
     gene            13057..14301
                     /locus_tag="PKI50_05920"
     CDS             13057..14301
                     /locus_tag="PKI50_05920"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNT03283.1"
                     /translation="MELTVQRARNIINALKNGVVPDCDLELLCVGRDREIKEFQRCFQ
                     VVSEGSGTVKFLNGEYGSGKSFFMNVIKQAAVKNDFIVSKVQVDRGFRFNNLEHLYYH
                     IMHNLALNIAEGSAASFESIFDLWISKLQESSNKDSAAAEINHVISSLNRYNMSFARA
                     FLAYIRARIEKNTVLSDTVASWLMGEKNIPASIKAKFDVVGSVDRLNSIDFLKAFVKL
                     IKLLGYSGLIILIDELELIMNERIDIRRVSYENLRNIIDVCCSGELGSCMFVFAATND
                     FFENPEKGIKTYEALSQRLGDAIDKSNSALYDVRQPVIRLHKLNTEEFQELTDRVINI
                     YKMLYNFVPANSNESIRSWTFISLRKFGGKLAELSIREYLTKLIEVLDIMEQHPENKL
                     FTSELKAVKKGDTEVYINRNRG"
     assembly_gap    14407..14506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14570..15283)
                     /locus_tag="PKI50_05925"
     CDS             complement(14570..15283)
                     /locus_tag="PKI50_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT03284.1"
                     /translation="MLKIDNLVVAYGGIEALKGISLEVEEGKIITLIGANGAGKSTTL
                     RSIMGLVKPKQGEITYMGKNLLSMKTQEMVRHGITLVPEGRRIFPNLSVLENLRVGAF
                     ARNDEKGIKEDIEMVYGLFPILKERSWQTAGTLSGGEQQMLAVGRALMSRPKLMMMDE
                     PSLGLAPLIVKEIFNIIKEVNKQGVTILLIEQNANAALHIADLGYVMETGRITLKGTG
                     AELLANPDIKKAYLGAEAH"
     gene            complement(15276..16082)
                     /locus_tag="PKI50_05930"
     CDS             complement(15276..16082)
                     /locus_tag="PKI50_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT03285.1"
                     /translation="MNVLRTDNITMQFGGLTAVKEFNLELNKGEIVALIGPNGAGKTT
                     AFNMITGVYTPTKGKVFLNQKSTVDGKEEHKERDITGLRPDQITKLGIARTFQNIRLF
                     KDLSVLDNVLIANHLRIDSNVFEAIFKLPRYKREEKQMVETSMSLLEKVGLADSRYEK
                     SSSLPYGQQRRLEIARALATDPSILLLDEPAAGMNPKESEDLTVFIKEIRDEFDLTVF
                     LIEHHMQVVMGISDRIYVLDYGITIAQGNPYEIQNNQRVIVAYLGVDEDA"
     gene            complement(16082..17122)
                     /locus_tag="PKI50_05935"
     CDS             complement(16082..17122)
                     /locus_tag="PKI50_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT03286.1"
                     /translation="MKRNTIYTITAIIVLFVFVYFGNKYLDSYLLRILNLCAIYTVLG
                     LSMNLINGFTGLFSLGHAGFMAVGAYTTAMLTLPIATKQMNFFLAPLVSPLDKIVWPF
                     LPSLLLAGILSAFVAFLIGAPVLRLKGDYLAIATLGFSEIIRVVFTNTQTITNGALGL
                     KGIPATTNLWWSFGTAVITVFIMVSLINSSFGRAFKAIREDEIAAESMGISLFKHKVL
                     SFAIGAFFAGIGGGLLGNMMGTIDPLMFRFFMTYNILLIIVLGGMGSITGTIISAFII
                     TIAQEVLRFLDEPIFPNVPALPGLRMVVFSILLMLVVLFFRQGIMGTREFSWDKFFRF
                     FTKRPLSKGGAK"
     gene            complement(17125..18018)
                     /locus_tag="PKI50_05940"
     CDS             complement(17125..18018)
                     /locus_tag="PKI50_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT03287.1"
                     /translation="MTLELFLQHLANGISLGSLYALVAIGYTMVYGILRLINFAHGDI
                     FMMAVYFAFFGVMTFGMPWYVSFIIAIALTGVLGITVETLAYRPIREAPRISILISSI
                     GASYLLENLAIVIYGGRPKTFPQIPLFTDMVQFGTVHIQMLTFMIPVVTAVLLMLLMY
                     LINKTKSGMAMRAVSKDFETAKIMGIDVNRTISFTFGIGSLLAAVGGIMWGLKFPSIL
                     PLMGVMPGIKCFIAAVIGGIGNIQGAVIGGFILGIGEILLVAFLPELSGYRDAFSFIL
                     LILILLFRPTGIMGEKISEKV"
     gene            complement(18411..19586)
                     /locus_tag="PKI50_05945"
     CDS             complement(18411..19586)
                     /locus_tag="PKI50_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT03288.1"
                     /translation="MKKLLSLTLVVAMALSLLTGCAQPAPAPSGEPAAPAEEEVIKIG
                     VFEPLTGANAAGGELEMEGIELAHELYPEVLGKKVELIVVDNKSDKVEAATAAARLVE
                     KEKVTAIIGSWGSSLAMAAGDIVKNAKVPAMGTSCTNPLVTQGNDYYFRVCFIDPFQG
                     VVMSNYAYNGLGAKKAAIVQEVSNDYAVGLAKFFTDNFKKLTGDENSIVAVANYNTGD
                     QDFTAQLTNIKAQNPDVIFAPGNFTESALLIKQARQLGIKTPFIGGDTYETPEFAEIG
                     GDAVEGVVFSTFFATERPITSESTKFLDAYRAKYPGKEPAAVTALGYDAYLILIKAIE
                     QAGAADSASIHNALLNIKDFQGAAGVVNFDENGDAVKSAVIKTVKDGKFTFMDVVEP"
     gene            complement(19843..20577)
                     /locus_tag="PKI50_05950"
     CDS             complement(19843..20577)
                     /locus_tag="PKI50_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT03289.1"
                     /translation="MIRSKSLYGTLMIFLFWYILYIMLQSPVIPSPFAAVLNFITILP
                     AVLLPHVLASLARIIAAAAFSLLLGLPLGLLTGMNNKADRLITPIVYILYPIPKVAFL
                     PVLMILFGLGNASKIILISIIVIFQIIVTTRDGVHELGKEYFLSAESLDMSRAQLYRH
                     IIIPAIMPKIITALRLTVGTSIAVLFFSENFAARYGIGYFIMNSWVVVNYVEMFSGIL
                     ALSIMGLLLFKIIDKLESRFCAWMEV"
     gene            complement(20579..21307)
                     /locus_tag="PKI50_05955"
     CDS             complement(20579..21307)
                     /locus_tag="PKI50_05955"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT03290.1"
                     /translation="MIEVNNLQVSYGNVRALEGISLKVERNATCAIIGPSGCGKTTLL
                     YTLAGLIRPEEGTIYIGGEELSGIRRSTGIILQDYGLFPWKNVWNNVALGLQVRGETK
                     EKISETVSNILAVLGIGHLSCKYPAELSGGEKQRVAIGRTLAMKPDLLLMDEPSSALD
                     AITKEHIQNLILKLYKSAPLTMVIVTHSIEEAVFLGQKIIVMGKAKIKHVLDNPYFGD
                     EELRSRMEYCKICLEVRKWMDRGE"
     gene            complement(21304..22227)
                     /locus_tag="PKI50_05960"
     CDS             complement(21304..22227)
                     /locus_tag="PKI50_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT03291.1"
                     /translation="MKKLKAVIIMLICTAIAAAGCSTADNKEPDITEIPKLKIGVMPA
                     ADSAPIFLAEEKGYFKELGLDAEIQVYTNAANRQSALQSGELDGAMVDLIAFVNNVHN
                     GFDIKITTSTDGSFPFLVNKDLKEKKNIKVGMMEVSVTNFLSEEYLGDKYEMEKVYIN
                     EIPARLEMIKAGNLDIANIPEPMASMGELNGLKKVVYENKDDFMPEAMAFTGKALKEK
                     EKAIELFHRAFDKAVDDIKRDDTLAREVLIEKLQLKPGIKDKIILPVYHKTRLPDEAY
                     IRKIVEWVEKIQNIKVNVKYDDMIEGKFINQ"
     gene            complement(22326..23735)
                     /locus_tag="PKI50_05965"
     CDS             complement(22326..23735)
                     /locus_tag="PKI50_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03292.1"
                     /translation="MGITLTTLHWVYLIFILLVIAFIVMRKDTSFICVAGILVLGFIA
                     TSTITHSVTGVFNSFAYAIKELLGTILIISIIYGMSKVLMKTGINEAMVYPFTRLIRT
                     PALAYWVIGILMMIISWFFWPSPAVALMGAVLLPVALKAKLPAMGVAVAMNLFGHGIA
                     LSGDFIIQGAPKLTADGAGIPVIDVVAASVPLVIIMGAVTTISAFWILRKDLKSGALK
                     VEDENMRKSMIVQINDGKASMKEIPPVVKKLLAAMVPVLFAVDILMMFILKLQGGDAT
                     ALIGGTSVLILIIVSFFAYSADPLGKITEHLIEGLQFGFRVFGPVIPIAAFFYLGDSA
                     IIDIFGKVLPEGSNGLVNDLGVALAGAVPLNRGIGAVTATAVGAITGLDGSGFSGISL
                     VGSIAKLFSIALGTGAATLTALGQVAAIWVGGGTLIPWAVIPAAAICGVDPFELARRN
                     LKPVVIGLIITTIAAVFLV"
     gene            complement(23929..24330)
                     /locus_tag="PKI50_05970"
     CDS             complement(23929..24330)
                     /locus_tag="PKI50_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03293.1"
                     /translation="MYDDEKDDIELDYELCESCPFHRQRPPTFGPFGPQFQPPGVGPG
                     SSDGPPSGPPPSITPQFGGPQGIGTLAVDPGSIRRCRFSFVYLQLRNGREFWMWLTFV
                     GRRSISGWRWTGRRWVFFGVDLRQIESFYCY"
     gene            complement(24408..25415)
                     /locus_tag="PKI50_05975"
     CDS             complement(24408..25415)
                     /locus_tag="PKI50_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03294.1"
                     /translation="MLSLVRGGPKVLLLESSRIEELRNYFIQEKGAEAYNPSDVFENA
                     GEDHTILFIIDKIKEAVDFDEADNGILINEEPDVILCNMLNDKKIDLVSRSRIATRII
                     VLRAMGDLERVVRDISGDYQCIVGSPLDIIKSSDEKGTLVVVTEKPLHRNISYKKDIY
                     EKGLLINEKYKPLIRSLRSRALKYLNNGIGNKDWYELEIRIYDKYGEYDLHYTRLINI
                     MESLEIGIILGASWGKDYPRPMMAIEIYRVRFFTYYDPIYIKKLLMGLEFLEDGTRIV
                     DYDLFYKRKKLYWAETVEKDIKERHLIAAKYRKEILSRLNEEEINKMYEIENNILATR
                     K"
     gene            complement(25442..26056)
                     /locus_tag="PKI50_05980"
     CDS             complement(25442..26056)
                     /locus_tag="PKI50_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT03295.1"
                     /translation="MLSFKKFIYNTIKKLYISSELLKSEEKKLLHISDTPLCFYSELK
                     RLIGWLKPAYIVHTGDLTDNIKLELYPALIHEHEKGVKVLADMLEASGAEVILAIGNH
                     DNIDVVNKHFVRSRIIIEAETINLEGLSFRISHFPKGVQENPSRYNLFGHDLTLKNGC
                     IDDKLYFNGITYINIIGLESGEFTTLHYPLCTNDARVGRRKVGL"
     gene            complement(26162..26983)
                     /gene="speD"
                     /locus_tag="PKI50_05985"
     CDS             complement(26162..26983)
                     /gene="speD"
                     /locus_tag="PKI50_05985"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNT03296.1"
                     /translation="MLMKAENGKLKLYGFNNLTKSLSFNIYDICYTKSREDRKKYIEY
                     IDEQYNSTRLTKILSDVTEMIGASVLNVAKQDYDPQGASVTLLISEEEVPLYVLDPSC
                     NRGIITPIRENIVGHLDKSHVTVHTYPESHPQNEISTFRVDIDVTTCGTISPLKALNY
                     LIGSFDSDIITIDYRVRGYTRNIRGRKHFIDHKINSIQNFIAKDTINRYDLIDMNIYQ
                     SNIFHTKMRIRDLDLNNYLFEIEEDDLTPKEKKDISKKLDREITEIFYGINLPKI"
     gene            complement(27107..27991)
                     /locus_tag="PKI50_05990"
     CDS             complement(27107..27991)
                     /locus_tag="PKI50_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT03297.1"
                     /translation="MKDQAVVSGSRRGYLNKDFEFFHLKDRKIMEFELHFHDFNKIII
                     FMSGNVTYLIEGRAYKLKPWDMLLIGSSEIHKAIVGTSGTYERMIIWVNSNFLHKYKS
                     PEGDLLRCFDIASKEKRNILRLNPKLLRQGKHILSQLKEECKSGGFGSQVLKNSLFLQ
                     FIVYLNRIYLEPDSIEHKADGDYDESIGEVIRHINENICEDMPIEGLASKFYMSKYHL
                     MHKFKSQTGYTIHSYILQKRLMMANTLIKSGKSVAEACTECGFGDYSSFVRAFKRMYG
                     LSPKKHIKMVEELEKAGK"
     gene            complement(28139..28267)
                     /locus_tag="PKI50_05995"
                     /pseudo
     CDS             complement(28139..28267)
                     /locus_tag="PKI50_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(28478..29434)
                     /gene="menA"
                     /locus_tag="PKI50_06000"
     CDS             complement(28478..29434)
                     /gene="menA"
                     /locus_tag="PKI50_06000"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNT03298.1"
                     /translation="MSLKSFLKLVEIQTKVASVIPFALGTLYTVYHFGIFNPRNFIIM
                     FISLLSFDMATTAINNYYDYKRANKTQGFNYESHNAIVKYNLKESTVKLTITALLTVA
                     SVFGLMLFLNTSPVILLLGVLSFAVGILYSFGPIPISRTPFGEVFSGLFMGFVITFIS
                     IYVHVYDTGIAGLTYSGSMVGISFNLTEGIYIFLLSLPAVNGIANIMLANNICDIEDD
                     IENRRYTLPIYIGKERALWVFKALYYISYADLLLLLVLGVTPAATVITLFTIFPVSSN
                     IKSFFEKQTKKDTFILSVKNFLIINVVQVLAIGTTIIIEGLI"
     gene            complement(29461..30144)
                     /locus_tag="PKI50_06005"
     CDS             complement(29461..30144)
                     /locus_tag="PKI50_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03299.1"
                     /translation="MKEIIKRMAKLFFGLFLYGLGIVMTINANLGLAPWEIFHQGLSI
                     KTGITMGQASIYVGMVLVGLDSLLGERLGWGTLFNMLFIGLFMDILMLNHLIPVFQSV
                     ILRFAMMLLGMFVIGIASYFYISVGLGSGPRDGLMVALTKKTGKSVRFIRNSIEITAL
                     AAGYILGGSVGIGTAIMAITAGYFVQFSFKLFKYNLKETKHRFIDEDLKFLYDRVRGK
                     KEDRKAEDQ"
     gene            complement(30141..31160)
                     /gene="galE"
                     /locus_tag="PKI50_06010"
     CDS             complement(30141..31160)
                     /gene="galE"
                     /locus_tag="PKI50_06010"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNT03300.1"
                     /translation="MAVLVTGGAGYIGSHCVCSLIDRKEKVVVADNLQTGHEGAVHGE
                     ARFYKGDIRDGKFLDKIFNENDIDTVIHFAADSLVGVSMKEPLSYFDNNVYGTEVLLS
                     AMNRHNVNRIVFSSSAAAYGEPESIPILETDRTEPTNAYGETKLVMEKMMKWADIAHG
                     IKYIALRYFNVAGAHESGRIGEDHCPETHLIPLILQVPLGKRESISIFGDDYDTYDGT
                     CIRDYIHVTDLTNAHIMALDRLRDGAASTVYNLGNGEGFSVKEIVEAARRVTNHPIPA
                     VICPRRAGDPSTLIASAKKAGDEMGWQPRYNSIDEIIATAWKWHKNNPEGFKGKRIVE
                     GDRQK"
     gene            31411..33018
                     /locus_tag="PKI50_06015"
     CDS             31411..33018
                     /locus_tag="PKI50_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HNT03301.1"
                     /translation="MQFDAHKYNYPSRRNIIYARKGMIAASHPLAAQAGLEMLKKGGN
                     AIDAAVATAACLTVVEPTSNGIGGDAFALVWSEGKLHGLNASGPAPASIPAEIIEKIQ
                     SRGIPKHGWMPVTVPGIPSAWSALSKRFGRLPLSEALSPAVAYAYEGHPVAPTVAKSW
                     ESAYRTYRIALKDELFRHWFDTFAPSGRAPKAGELWSSPAHGKTLQEIGETNAESFYR
                     GALAEKISEFAGKTGGYLSLKDLESFEPEWVEPISINYRGYDIWEIPPNGHGIVALMA
                     LNILKGFDFELRDSIDTYHHQLEAMKLAFADGIRYIADRRSMKVSTEELLSDNYAAER
                     RKCIGSKALIPTPGRPPEGGTVYLAAADGEGNMVSYIQSNYMGFGSGIVIPGTGISLH
                     NRGCNFSTDPSRENCIAPGKKPYHTIIPGFLTREGRAIGPFGVMGAFMQPQGHVQVIM
                     NTLDFHMNPQDCLDSPRWQWIAEKDIEVEEGFPLDAVQGLINKGHNIKINPYSGSFGR
                     GQIIWRDERNILVGGTEPRTDGAIAAW"
     gene            complement(33023..33430)
                     /locus_tag="PKI50_06020"
     CDS             complement(33023..33430)
                     /locus_tag="PKI50_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03302.1"
                     /translation="MKKRFVIYGLAGWIAETIFTGLGSLFKGDMTMHTWTYIWMFPIY
                     GLMILLEPVHDNIRNAPVVARGGVYTIIIFGIEYTTGWILREIIGKCPWDYSAWPYSI
                     DGIITLSFIPVWFFGGLIFEKLHDYLIRRKILM"
     gene            complement(33466..33678)
                     /locus_tag="PKI50_06025"
     CDS             complement(33466..33678)
                     /locus_tag="PKI50_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03303.1"
                     /translation="MKEFLSNNGIIYEYIDITDSMKNLKVYLKLRDTRPEFEEIKRIG
                     RVGIPFIMLDNGEKLIFDQPELDELK"
     gene            33953..34243
                     /locus_tag="PKI50_06030"
     CDS             33953..34243
                     /locus_tag="PKI50_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03304.1"
                     /translation="MELKEPLVCTKCSGSHFTVKREATYLYSYDLENKKSEALPFLFD
                     NRELIKSHEYLQCEQCGAKFPCTLDERSNTINFTIEQKAVRSDHVTNPEFLG"
     regulatory      34335..34435
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      34439..34513
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            34604..>34737
                     /locus_tag="PKI50_06035"
     CDS             34604..>34737
                     /locus_tag="PKI50_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNT03305.1"
                     /translation="MSEPKKTSLYDVHEKYGGRVIDFAGWALPVQYEGITAEHEAVRN
                     "
     assembly_gap    34738..34837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            34918..35301
                     /gene="gcvH"
                     /locus_tag="PKI50_06040"
     CDS             34918..35301
                     /gene="gcvH"
                     /locus_tag="PKI50_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNT03306.1"
                     /translation="METKSGLLYSKDHEWVRVEGEKAYVGITDYAQHALGEIVYVELP
                     AVGDELNAGDVFSVIESVKAASDSYLPIAGKVLEVNAALSDSPQLVNEDPYGSWIVVV
                     EITDKAGLDELMSEQEYREFCDKEA"
     gene            35303..36643
                     /gene="gcvPA"
                     /locus_tag="PKI50_06045"
     CDS             35303..36643
                     /gene="gcvPA"
                     /locus_tag="PKI50_06045"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNT03307.1"
                     /translation="MHRYLPHTEDDERLMLESIGAKSTDDLFVDIPRELRLGRKLNLN
                     NPLSEIELSDHMKGLSNKNKSSDDLVCFLGAGAYDHFIPSVVKHLAMRSEFYTAYTPY
                     QPEISQGTLQAIFEYQTMICSLTGMDVTNASMYDGATACVEAAMMAVENARRSSIIVS
                     RTVHPEVRKVLKSYMRFRDVEVIEADMADGITDVEKLKPLVEAGTAGVIVQNPNFFGI
                     IEDLTEIEKMVHANKALLIDYADPISLGILKSPGESGVDIVAGEGQSLGNSLSFGGPY
                     LGFLATTSKLMRKMPGRIVGQSEDADDKRAFVLTLQAREQHIRRYKATSNICSNQGLN
                     ALAAAIYMTTMGKKGLKEAAEQSAKKAHYAMKQLTSSGKFKPLFNKPFFKEFAVTGET
                     PAGKINDELLKHNILGGFELDKEYPELKNGLLFCVTEKRTKAQIDKLAQVMEVV"
     gene            36640..38115
                     /gene="gcvPB"
                     /locus_tag="PKI50_06050"
     CDS             36640..38115
                     /gene="gcvPB"
                     /locus_tag="PKI50_06050"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNT03308.1"
                     /translation="MMKNYNSLIFELSKEGRKAYSLPPCDVSDISLDSVIPPSLMRDS
                     EPELPEVSEVDVVRHFTNLSNKNYGVDTGFYPLGSCTMKYNPKINEDMANLSGFSNIH
                     PLQPEATVQGALKLMYEFDGMLSEISGMDRVSLQPAAGAHGELTGLMIIKAYHESRGD
                     KKRKKIIVPDSAHGTNPASAAVAGFEIVEIESDKNGAVDLDRLKAVLSDEIAGLMLTN
                     PSTLGLFEKNIKQIANLVHEAGGLLYYDGANANAIMGIARPGDMGFDVIHLNLHKTFS
                     TPHGGGGPGSGPVGVKSELARFLPVPVIEKKGDSYILDYDRPDSIGKVKSFYGNFGVA
                     VKAYAYILSMGGEGLTDVSRKAVLNANYMMSKLKKYYELPIDQVCKHEFVLSGLLEND
                     NHIATLDVAKRLLDYGYHPPTIYFPLIVNNAIMIEPTETESLETLDSFIDVMIKIAEE
                     AKEAPDILKTAPHGTVVRRIDEAKAARSLILKWDGTCMNKE"
     gene            38121..38612
                     /locus_tag="PKI50_06055"
     CDS             38121..38612
                     /locus_tag="PKI50_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03309.1"
                     /translation="MNPFIKKLADSKFMTKAQGNYIEEAVMRKDSIIISGHKGFGILP
                     LFATISATAKSNFKIKQIKGFEDLGDEADYYLIADLSNIDYAKLIAEAMLKQGSSFIA
                     LKDPDHPYSVFKILGDVYKINGDTSKVYQLIECVKIDGVPQLSKITRITLNENGKPVK
                     TDF"
     gene            complement(38670..>39029)
                     /locus_tag="PKI50_06060"
     CDS             complement(38670..>39029)
                     /locus_tag="PKI50_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HNT03310.1"
                     /translation="DLWRQINGFIQQRYKSLPRIMYSVCSGKPGWNVKYQKSGKSICT
                     LYPEQDGFVALVVVTLDLMPVIEALSGELTEETRQTIRSAKPFNGTKWLMLQVKNDLQ
                     LEDVKQLISLKQGAIAK"
BASE COUNT        10854 a         9024 c         7316 g        11535 t
ORIGIN      
        1 cctgctgttt agattatata cccactgctt tttaagcctc ttaacgccct cgatagagag
       61 tccacccttc aaatccggtg acaaatctta ctgttgaaca agtagcagag aaaccatact
      121 tactgacaga tacccaataa gccgttcatt ttacttcact gtttacgata aacccgatag
      181 ttcgatgaaa aatataaatc aaggtgattt tcttgataag attaattatt ggggcggttc
      241 tgctatggct ttcatatcct ctgttatggt aatagtcaga tggttcaaag gaaaaaagaa
      301 aaaagaatcc gacagcggca taactgaaga gtcaaatgag caaatctgcg ataacaatga
      361 atctgcttaa accggtccaa tcatctataa atttacttgc ttaacgccgc ctcaaccgcc
      421 ttaagcaggg ctttgcttgt cgtagttgcc cctgatactg catcaacatt ggttttctgc
      481 ttatcaataa ttctatcgat tacagcctca gctttcttac catattcagg agtaccgcat
      541 tccaggacat ttattgcctc tatcctgtga ttatttacta taacctcaac catgcagcgt
      601 gtattattat agctgaattc cccggtatat ttgccgtcag cgatctcgtt caagtcaata
      661 tcttttattt cagcatttct tacttcatac aaaaccctat tggccggtgc aatattgata
      721 aaataaaaga ccccgaatcc gattgctgca atgcaaatca gtatgccgat cattttataa
      781 tttttcttgc tcatcatagt acccctttct aaagtattca ataactgata cgatataatt
      841 ctagaaaagt ctgatatatc ctgcaaaagc atctattagc ttgaataaac agcctaatca
      901 aaggtatacg ttatcagaaa aaacttgtcg aattatgata cttttttctg attacagaaa
      961 aaactttgct tttcggtatt tttttgttat actcatttta agagaggtga ttataatgat
     1021 acaaagaaaa gaatacttaa ataagcttat agagtggcgt gaaaagcagg tcataaaggt
     1081 cgttacgggt gtaagaagat gcggcaagtc taccttgttt tccttgtaca ttgactattt
     1141 gaaaaaaacc ggcgtatccg aagaacagat aatattgata aaccttgaag atatcgaata
     1201 tgaaaacctg ctggattaca ggccgcttta tgattatatt aaagcaagaa tagtaaaagg
     1261 aaaatatacc tatgtcttca tagatgaagt tcagcagtgt aagtcttttg aaaaagcggt
     1321 tgacagctta ttcttaaaag aaaatgtgga tgtatatatt acaggctcaa atgcaaccat
     1381 gttgtcagga gaacttgcta ctctgttatc gggacggtat atagaaatct ccatgctgcc
     1441 gctgtcattt tcagaatacc ttgagtttgc caagggcaga gatgtcaaat caactttcga
     1501 tgaatacttg aaattcggat catttcctta tgtagcagtc ctaaataaaa gcgatgccgt
     1561 tgtcagaaca tatattgacg gtatttataa cacaattctg attaaggatg tcgcagcccg
     1621 tgaaagaata aaagatatat cggttcttaa aagcattgta aagtttttat gcagcaatat
     1681 aggaagccca gtatccgtaa aaaaaatcag cgatgctata aatgcaagcg gacgcaaaat
     1741 atcggttaat actgttgaaa attatcttcg cgccctgtac caaagctata tattttataa
     1801 agttgacaga tatgatatta aagggaaaca gcagctgaaa actcttggta aatattatat
     1861 tgtcgatacc gggcttcgta atctgctgtt atcaagttca gcagccgaca tagggcattt
     1921 gcttgaaaat gtaatatatc ttgagcttat tcgcaggggg tataaagtaa atatcggaaa
     1981 gcttgcagaa aaagaaattg attttgtagc aggcaatact gaaggcacag aatactatca
     2041 ggtgtccgca tcggtgcttg atagaaacac attacaaaga gaacttgagc cgctgcaaaa
     2101 aatacccgac caccacccaa aatttttatt aaccctcgat gacattatgc caaatgctaa
     2161 tcacgatggt atccgccaga taaatgcaat tgattggctg atcaagaaaa aaccttgatt
     2221 tctcaaggtt tctgataatc ggcaaatatg gtgcgcccga caggagtcga acctgcgacc
     2281 ttctgattcg tagtcagaca ctctatccag ctgagctacg ggcgcattat tgattttgaa
     2341 gttcattttt cattataatt aaatgcctat gataagtcaa tgtataaata aggctattag
     2401 taatcgacac gtacgccgtc aactgccaaa tcctttatcc ataagaactt tctcaaaaaa
     2461 atatgaatct ggtcgttgcg aaccctggtt ccccttttta tatatgcgtc aactccatca
     2521 acactataca aatcaaagtt ttcggctttg tccggcttcc ccaactgaac tgtcggagct
     2581 tgtattgaac atcagccgcc gcctgccgca tgtatatata gggtcactga gctatcttta
     2641 gtatgttttt ttatataagc tgcagcatct ttttcaataa ttaagttcat agaattcacc
     2701 tcgtttctaa cagcgtctgt tgaaaaaaac ccgtaaaata tttaatttta cgagtttcat
     2761 cctaatatat attatatcag acttgcatga aatctgtctg ccattttatt ctgcggcctc
     2821 aatttcttct tcactttctt cttcaatttc tataatgtcc tcagtgtttt ttgcttcttc
     2881 cttcttcttt gattttgaaa gcatataaac tcctgctcct atcaatgcaa taggcattat
     2941 gcttcttcta attgaccaat aaagatctcc tccaaagaca agctgaaaga ctgagcgtcc
     3001 aaaagcattt actactccca gaattcccag gaacagcatt accataccta caatcctttt
     3061 attctctata agtatgttat aatcaaatat aacactgtct tctacttctt ctcctttttt
     3121 aatccttctt ctcatggcat agctgtcgaa gaagctgaaa aaccaggtag gtattatgat
     3181 aagcattatc atgccttcag tattcaagta actgaacaat acaatagccg aaatgaaacc
     3241 gaacatcagc tgaaagcctc tcttgaaaag gcccatatac atttgggctg ctcctggaac
     3301 tacagcaaaa cagaaaaatg caagattttc gaagaagccg ggcttttgat actgcctgtt
     3361 ttctgagaac agcgactttt gaatgcagct gctgcatatc ttatttccgt ttacatcaac
     3421 ggagcattcc tcgcaaatcg gctgtttgca tttatcgcag atgaatttcg cttccgattc
     3481 tttatgatat ttacaattca ttttattttc ctccctcttt ttctctttct atcctgccca
     3541 taatcccgtc cagctgagta atactgtttg tcagcttgtc tattcgctca tttattgagc
     3601 tgaagggttg agatatcttt tgcgtgatgt tttccccggt attatacagc tcattctgta
     3661 cgcgcaccag atcatctttt ggaactgtat tcagaagcaa gagtatcaaa cctgctgcaa
     3721 caaggctcat tcccaagttg gccatttccc ccagggtatt cctgtatgat tttttgtacc
     3781 ttttgagatc tacagaggcc ataatggagg atttcaaatc cacggatggt atgatacttt
     3841 cctgaaaacc tgacttcagg aatactttca gcgccttaat ttccgccagt tctcttctgc
     3901 atctgtcaca ggacttaaga tgccttgcca cagcatagct ttccagttcg ctaagctttc
     3961 ccgctgcata ttcatgaagc attcctctat tttcatcgca gcacattttg ttcacctcca
     4021 caaatcaact cttccatttt aagcttcaac aggttctttc ccctgaacag cctggtatct
     4081 atggtacgct tgggaacacc ggttatttca gatatttcct ggggactgaa gtcttcaaaa
     4141 taataaagta taattacggt cttataaatc tccggaaggc tttccacaag ctttctcaaa
     4201 tatactgcat tctcctgctt tatcaggtct tcttcaggcc ctccgccgct gtctgctata
     4261 gtatcatata tatccagctc ctcttcgctg ccgtcacaga tggctgatac tgtttgcggc
     4321 ttctttttcc ttgcccagtc gatacatcgg ttcactgcaa ttctgtaaag ccaggtggag
     4381 aaagaggctt tgcccttgta tttctgcagg ttgttgtaaa gctttatgaa tatttcctgt
     4441 gtcaaatccc ttgcttcctc gtaatcatgg gtatagtgaa aggcggtgga aaataccttt
     4501 gattgatatc tgtcaattat cacggagaat aactcagtct tgctttttac tatgtacgaa
     4561 accacttctt catccctcaa tacccatcac cttcctttac ctgcccatct atttattaat
     4621 acgaatcagc ttctatccat acttcaaagg cgcaaaaaaa gaacaagtta ttgttctttc
     4681 atgcaaaata ctccattact tcattgaaac tgtggatata tccttccgcc aactctatca
     4741 gtttatcctt cgactccagg gatatttcat ccgccatggc aaacactctc attcccgccg
     4801 actttgctgc catcacccct gaaagagtat cctcgaatac aaggcatcgg catggatcga
     4861 ttgccatatc ctcagccacc ttcaggtata catcgggata cggctttcct ttgtcaacct
     4921 cacaggaggt tctgatattg gaaaagtact ttcttatccc atgatttgca agcatacagt
     4981 ctacaagctc cctggaattg ctggtagcta ttcctaatgt caagccctta tcataaagca
     5041 gctttaagaa ctcctttgcc ccgcttttca actggatatg gcatttgtaa taatcctcag
     5101 ccataagccg ccattcttcc tttatctcct ccaaggattc aggaaggttg aacctttcct
     5161 tgaaataaac cgcagtttcg gaatagctca ttccttctat ctccatttgc agcctttcgg
     5221 gaagcggaat accccttttc cccaggaatt ctatatccac ctgcttccat atccacatgg
     5281 aatcgatcag ggtaccgtcc acatcaaata ttacagcctt tatatcctta tacatatgcc
     5341 ctccgtatct attattatca atatattact caaactaaac ttattatagc ataatagaaa
     5401 aataatgcga ctaggacatg gggagggaca cggggacgtt tcgcgtgtct cgctgaaagc
     5461 gggacagggg aaacgtcccc atgtccctcc ccgtgtcctc tttgagcctt attctataac
     5521 aatactccca ttattagttt caagctctat tttgtattgc cccgttccca gccttccctg
     5581 tgcgacggta tcctgccctt cctttttaat ctgcagcggc ttattgcaat taatacttcc
     5641 gaattttgtc ctggcatata tatctccgtt atctgcactt gcagatttgt aataaatatc
     5701 cccgaaggca ttctttatct gcgcatttcc ggctacatcc tccgcttcaa tccttccgtt
     5761 cttgttttcc acagtaatgc cgccgtcggc cctttttatg cctatgtctc caaaagcatt
     5821 aaccaggtca gctttgcctc caacttcttt gacgtatatc cttccgttgt tgtttactac
     5881 cttcaaatct ccgccgactt tttccgcttc aatatcccca aaatgatttt caattacagc
     5941 agaaccgccg atattgtctg ctgatatctc cccgttatgg ttggatatat ccgcctttcc
     6001 tcctatattg ctaacttcga tatccccgaa ggaattgtca atatccacat ctccgcctat
     6061 gtttgaagct gttatttcac catttttgtt ctcaattctg cattctcccc ctattccttc
     6121 cgcttcgata tctccaaagc tcccgcgggc tatcaggctt atttccttgg gcacgtatac
     6181 cacaaagtca acctgcgctc ttgcatgatc attcttattc cagttgggcg ggattttggg
     6241 agaaagctcc gtgacttccc cctcaattat ttcaacggca tttcttgcat attcccttgc
     6301 cttgttctca tcattgcact taaccattac ctccgcttcc agcctgatgg tatttccacc
     6361 ggcaggcaga atcttgattt cgccgaaatc attggttact ttaagggtct taacattaaa
     6421 gccgccggat atattatcct tgctatattt ttcgctgatc tccgtcctgt acttaaagcc
     6481 ttcaaacatg gagcttccgt tgaatccgaa gaaatggcgc cccggaatat taatatcgat
     6541 gaatctgatt ccattggata taattccgag aattattatg aagactatgc acaacccatc
     6601 aacgctgagc ttgacttctg ttttgttcct tccgtacagt accatataaa gtatcatttc
     6661 caaacctaat ccgattaaaa gcaccggcca gtatttatat atgtccttta cgggtatatc
     6721 cataaaattt tgtgcaaaca gcagtgcacc cacagtaatg agtcctacgg ccagggttat
     6781 gctgcctacc ttccgtctta tcatttcaat ctatccctcc gatctattta ttttgcaatc
     6841 aataaattac ttttcattct tgcgatttct atagtatact acggatacca caggaaaaag
     6901 tctaatatat ttcggaaatt tataacaaga tttcaataaa cgaggcggga gatatgctca
     6961 tgacctgttg ggagacatct tatcgcttcg ttattagtat agactttaag tatatgcttt
     7021 ttacttcaaa aaagagtcct gcggatctgg caaaatccac aaggctcttg tcatttcaag
     7081 cctgctggga atcttgggtc aagatactta gcgaccctca gtatgacagc acttccttcg
     7141 aagccctacc tcaactctcg tgcctcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnntcc tccaggtatt tataatacag atctccatta ccctcaccat attccatttc
     7321 tgtaaaaacg ccctcggagt aggtggcaaa gccttcatcc agccatgctt catcaatctg
     7381 gtcattgccg acaacaccat accaccactg gtgagctgtc tcatgaactg tggtatacat
     7441 aaaaatatcc gcaccggaat cattatcgta atactttgtg cttatatata caagtccggg
     7501 atactccata ccgctgggga attccgtctc aacaatcgag tatgtcggat aggggtactc
     7561 accataggcg ctgttgaaaa cctctatgga cctcttccca atgtccagag cttcctttcc
     7621 tctcttttca tgatctttat agtagtatga cttgactaca gtatttccga ccttatcctc
     7681 agctacccgg aaattattgg aggcgacaaa tgcaaaatcc ctcatattgg ctccatggaa
     7741 cttccaattt ttatatccgc cttctattct ttcttccgcc aaggaaccgg aagctgctat
     7801 gatatattcc tcaggtgcct ttatactcac aatataatcc gaaacgtcac tgtagaaggg
     7861 atctcctata gagtaatatt tatccagatt ccatccttca tcatcaaata cagccgccac
     7921 cggataccaa ttccccatgt ttatattggc atctccatag ccgaaccttt ctcccgcagg
     7981 gggaatgata accttatact taaaccggag atttacactg ccctccggct tcagaggctc
     8041 agggagcttg attctgagta ttgtttctcc gactccctga aggctgtatt caagggcgtt
     8101 ccgattctgc ccctttgaaa actcaatggc ctcgatttct gtatatcccg gcttaaaacc
     8161 cctggaatag gccatggaaa aatcatcaaa gaggaacgga gctgtctctt gcttcctgaa
     8221 tgcattggcg tatatatgaa aataaagctc tgaaagttcc acatcatcat tattcacata
     8281 tataacctgc tgctctgctg tcagctcctt attagccgta tcaagaaccg catctataat
     8341 atactgattg acatccgttc ccttagctgt cggagaaggc acaacagcag gaaccggtat
     8401 gttcggactc tcctgcttta cggaacagcc tccggcaatc aatataaata acgctgtaat
     8461 tataaaaatt gtgctttttc gcttgactct tgaaatcaat aaaatcaacc tttctataac
     8521 aacttattta taccccgttc aataatacca tatacgcagg gatgaaaacc acgctcagaa
     8581 ttgaggttat tgtaaccata tatgttgcat aatattgatc cccgccaaaa gcagcagcta
     8641 ctattgcaga ctgtgcgata acgggcatag cggattgtat tacaaacacc ttggtcatta
     8701 agcttggaat aaaaataaca agtgtcagga aatatcccag catagtcagc actgccatac
     8761 ttaatacaga ttgaagcgcg tttaatataa catccattat atgttctccc atgttcaaaa
     8821 aataaattaa gccattttat gtcgtttaat tatcttgaat tttttcataa ccagatttgc
     8881 taaaggtatt aaatataaat aaaacgaata caagacataa ctgttgtctg cagttaatgc
     8941 ggcagcaggt accgccccgg caatattcca cgggataaga ggcgccatca ctattgcggt
     9001 attttcaaga tccactgcaa gctcatattt gtctatacct gcggaatcat acatatttct
     9061 aaccaattga tatgtcagca tcacggctaa ggtttggctg ccgccgaaag ctcccgttat
     9121 aatactggtt agaataacag cagcaaatat accggctttt ttcccgagtt ttataatcag
     9181 gttcccggca aatttcagca aggatgcgcc ttcaagcact ccggataatg aaaaggctat
     9241 cagtacaatc agtactacat taatcattga gataataccg cctccggcca ttatatctcc
     9301 caaaaagctt tgctccggcc tgctgtagcc cataataata tatttcagca catcccctat
     9361 ggtctccttt tgaaaaaata ccgcaacagc agccccgcac aatatactta cagccatgga
     9421 gatttttata tctaccttga aaaaggccag taccagtatt attatcgccg gcatagacac
     9481 tacccaattc aaattgtatg tctgtactat ccctgttgtt atccggtttt ctccaagctg
     9541 cagaggattc tgaaatgaca gtaccccgta tataattgaa gcaatcagga agggtactgc
     9601 agtggttctg aacatgtttt taatattcgt ataaaggccg gtctctgtaa gaacagatac
     9661 caggtttgca ctggaagaca tgggcgaaca tctgtctcca aagtatatgc ctgacataat
     9721 cgcacctgca acaatattga tatttatatc cccgcccttt accattacca taagtatcgt
     9781 gcctatagta ccaaccgttc caaaggaagt acccagcaaa aaagaaacag cacagctaag
     9841 caagaaagca tatagtataa aaagattcgg atcaacgaac cttatcccat aatatactac
     9901 atatgcaaca gttccggaag ctctccaaac agacataatg gcgcctataa gcatcattat
     9961 gcttattata agcaccgact tccttatacc ttttaaaatc atgccaataa tttcttttgg
    10021 cttataacct cttttatatg caactgtaat aaatattatc agccctataa ggagaggata
    10081 taaaatgttt attcccctgt atacgctaag catgagaagc ataaatgtga caataagtgc
    10141 aactgctaca tccaagctat tacctccagc cataagacaa aatctaagtt aattatatcg
    10201 catcagcaga caattttcga ttgcagaaat attttcttgc ctcttcgtgt ttttcaatta
    10261 tatgatttta gctgctcctc gccctttaat acccttgaca ctccctgaac cagtgctttc
    10321 atttcattct ctcccggata taccttaacc ggagcaataa atgccatcct ttcttttatc
    10381 catgaaacaa actccgtatc atgggctatt ccgcctgatc tttcaggaga gaaagggccg
    10441 tctccgtcca aggtattatt gacatctacc attctgcctt ttttgtgtgc ccctaccgag
    10501 ataccgccgc ctaaatgggc aacaacaaaa ttgtaatctg tgtaatttcc tcccagttct
    10561 tttgctgcca tcctggcggc agccttctga ttaaggggat gatccttgga ttttctctcg
    10621 atatccggta ttcccgaaat ccttgcatat tcctcaagtt catcaacaac cacaggatcg
    10681 acaacatatg aatggatgcc taactccgct gctataaagt aagctattat acctcctagg
    10741 tttgacgcat gctgccccat tacaccgagc cttaaatctt ccaccattgc ttcatttatc
    10801 tcataaacgc ctccttgtac gggcttcata ttccctcccc tgcctacaat aacatctatc
    10861 tcggataaat ccgtcccgga ttcctttacc gtgtctaaga ttactttttt catctgttca
    10921 acctccgata taatcatatt ttgtcagcaa ccaatactgc aacagctttc cctgcttatg
    10981 cacaagttat ctgccggact tcagctgtct tatttcagcc agtacccttt tatattccat
    11041 gtcgagctgt tctgcgtcat catcctttcc gggcatggac agcctgctta atatctccga
    11101 aagcctggta tcaagcaaca gcttctgtct cttcctttca gtttcaccgg aatccgctgc
    11161 acgcctcctg aattcaacat attcaccgta gcttccttca aaggtattca gtctgcggcc
    11221 ctcaatgtat attatcctgt cggcggtgct gctgataagc ttcctgtcat gggatgcaaa
    11281 aaagatagtg ccctcatgaa gcctcattgc attttccacc gcttccatag agcttatgtc
    11341 aagatagttg gtgggctcgt caaggataat gacatttgca tccgagcaga atatttttgc
    11401 gaagcaggtt cgtacccttt cccccccgct aagctttcct gcctttttat acacatcatc
    11461 ccttctgaag agcagccttg caagtatcgt ccttacaaag tgttcaggat atatgctttc
    11521 tttcatcaca ttttcaagta ttgtttcatc atcctttaaa atatccgttc cctgactgaa
    11581 atacccaagt tttacaccat tggcaagttt aattgactca tctcccccca ttatcatatt
    11641 gagaagagta gatttgcccg tgccatttcc ccctaacagg gcagttttgg atccgttcag
    11701 aacttcaaac tccgcttccc tgaacaattc cttcgagcca aaggattttg atacgccgct
    11761 acccgatatc agtactctgc ttacaggttt ttcggcaccc tgaatatcga taatagcgtg
    11821 ctgctgatcc ttcggttttt cctttttctc aagctttctg attctggtct ccatagcttt
    11881 gattgctttg tcaagattcg ctttagcctt ctggtttccc gttttatgaa gacgcgcctc
    11941 cgagtttccc atgcgggaag gtgctttctt catggaggcc gccctattct tcttttcttt
    12001 gacagtctct tccaatgccc ttttgctgtc aatatactga tcatattcaa attgagccct
    12061 tttgaattcc atctccttct gcagcttata ttgggagtaa ttgccgctgt actgcttcaa
    12121 ttctcctccc tcaatctcaa ctatcttgct gcaaagctta tccaaaagct ctctgtcatg
    12181 ggatacgatt aatatcaagc ctttatagct tgccagtttt ttttcaagaa gttctatccc
    12241 ctgtatatcc aagtttgaag caggctcgtc agctagcagt atgctgttgt tttccgaaag
    12301 gctgccggca atcttgaatc ttgtcttttc tcctccgctt gccgtatcaa ggctgttgaa
    12361 attcaatcca aattcctttg ccatacggtt gtcaacctct gaggccgcct ccaaatcaag
    12421 ctgcgttata tatgatatct ctccataaag cttgatactg ccttcctcgg gaatattcct
    12481 tcctgcgatt atatcaagga gcgttgtctt gcctgcaccg tttgctccta ctataccgat
    12541 tctttctccg taatatgcct taaagtcctt aatatccaat attagccttt caacgtaata
    12601 tttcttcagg ccgttaatct ccaataagag cataatcatt tcccccttgc atctctattg
    12661 tataagcatt acacttgtta atacaataaa aattcccctc ctgcttttcc ggagaggata
    12721 catagctgca gaaagcatgc ctgaagcaat aaccgcctat agcagcaaac atactgttat
    12781 ttataatata cataggacta tgtatcccaa ccggaactga atgaatagca gaataacccc
    12841 cggtgcgggg ataaatccgc taaatgttta gttgttcctg attagaataa tatcctcatg
    12901 tttttaatac cttacctttc ttcgtttcgt acttattata gtaaagcggc ttgtgaaagt
    12961 caagctgcgg gaaaatagtt attcatgttc cggaaggaaa ttgtttcatt atgtcgaatt
    13021 tactgactta gcataataac tcaagaggag cacgtcatgg aacttactgt acaaagagca
    13081 agaaacataa taaatgcact gaaaaacggc gttgtccctg actgcgacct ggaactcctc
    13141 tgtgtaggaa gagataggga gattaaggaa tttcaaaggt gcttccaagt ggtttccgaa
    13201 ggcagcggta ctgtgaagtt tcttaacggg gaatacggct cgggaaagtc cttttttatg
    13261 aatgtaatca agcaagctgc cgtcaaaaac gattttatcg tgtctaaggt acaggtggac
    13321 agaggattca gattcaataa tcttgaacac ttatactatc atattatgca taatctcgct
    13381 cttaatatag ccgaaggctc cgccgcaagc tttgagagca tatttgacct ttggatctcc
    13441 aagcttcagg agtcatccaa caaagacagt gctgctgcgg aaataaacca tgttatttca
    13501 tcccttaacc ggtataatat gtcctttgcc agggcttttc tggcatacat aagggcaaga
    13561 atcgaaaaga acacggtatt atcggacaca gtagcctcct ggctaatggg agaaaagaac
    13621 attcccgcaa gtattaaagc aaaatttgat gtggtaggca gcgttgacag gcttaattct
    13681 attgacttcc tgaaggcttt tgtgaagctg atcaagctgc tgggatacag cggccttata
    13741 atccttatag acgagcttga gctgattatg aacgaaagaa tagatatcag aagagtttcc
    13801 tatgaaaatc tcagaaatat tattgatgta tgctgctcgg gggaattggg aagctgcatg
    13861 tttgtttttg ccgccaccaa tgacttcttt gagaatcccg aaaaaggtat aaaaacttat
    13921 gaggctctgt cccaaagact gggagatgcc atagacaaat ccaattccgc tttgtatgat
    13981 gtaaggcagc ccgtaataag gcttcacaaa cttaataccg aagaatttca ggagcttacc
    14041 gacagagtaa ttaatatata taaaatgctt tataattttg tacccgcaaa cagcaatgaa
    14101 tccatcagaa gctggacctt catttcctta agaaagttcg gcgggaaatt agccgaattg
    14161 agtatcagag aatacttgac caaactgata gaggtacttg atataatgga gcagcacccg
    14221 gaaaacaagc tctttacttc cgaattgaag gcagtaaaaa agggtgatac tgaagtatac
    14281 ataaaccgaa atagaggata gagaagccga aaggacacgg agagggacac ggggacgttc
    14341 gggacacggg gacgtttctc ttgtcccatt gaaagtggga caagagaaac gtccccgtgt
    14401 cccgtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaaa cgtccccgtg
    14521 tcccgtgcat atgagaaggg gtatcgctat cgatacccct tcgtttcttt taatgggctt
    14581 ccgctcccag atatgctttc ttgatatcag gatttgcaag caattccgca cctgtcccct
    14641 tcagggttat tctccctgtc tccataacat atcccaaatc ggcaatatgg agagctgcat
    14701 ttgcattctg ttcaataagg aggattgtaa ctccctgctt gtttacttcc tttatgatgt
    14761 tgaatatttc cttgactata agcggtgcaa gccccaatga cggctcatcc atcatcataa
    14821 gcttcggcct tgacatcagc gccctgccta ccgcaagcat ctgctgctcg cctccggaca
    14881 atgtgcctgc tgtctgccag cttctctcct taagtatagg aaacaagcca tacaccatct
    14941 caatatcttc ttttattccc ttttcgtcat ttcttgcaaa agctccgact ctgaggtttt
    15001 caagcaccga aaggtttggg aaaattcttc ttccttcggg tacaagggtt atcccatgtc
    15061 ttaccatttc ctgagtcttc atgctgagca ggtttttccc catataggtt atttcaccct
    15121 gtttaggctt cacaagcccc attatggatc tcagggtggt actcttgccg gcaccgttcg
    15181 cccctataag tgttattatc ttcccttcct caacttcaag gctgattccc ttcaaggctt
    15241 caattccgcc gtatgcgaca accagattgt ctattttaag catcttcatc cacccccaga
    15301 tacgctacta ttaccctttg attattctgg atctcatagg ggtttccttg agctattgtg
    15361 attccgtagt ccagaacata aatcctgtct gagataccca ttacaacctg catatgatgc
    15421 tctatcagga ataccgtcaa atcaaattca tctctgatct cttttataaa tacagtcaag
    15481 tcttcacttt cctttggatt cattcctgcc gcaggttcat ccagaagcag tatactcgga
    15541 tctgttgcaa gtgctcttgc aatctccagt ctcctctgct gtccgtatgg aagggaactg
    15601 gatttctcat acctgctgtc tgccaggccc actttttcca gcaggctcat tgatgtttcc
    15661 accatctgct tctcttctct cttatatctt ggcagtttga atatcgcttc gaaaacatta
    15721 gagtcgatgc gaaggtggtt ggcaataagc acgttgtcaa gtacgcttaa atccttaaaa
    15781 agccttatgt tctgaaaggt tctggcaata cccagttttg ttatctggtc aggcctgagg
    15841 cctgttatgt ctctctcctt atgctcttct ttcccgtcaa cagtgctctt ttggtttaaa
    15901 aacaccttcc ctttggttgg tgtataaact ccggtaatca tattaaaagc tgtggttttg
    15961 ccggcgccgt taggcccgat aagtgccact atctcccctt tgttaagctc aagattgaac
    16021 tccttgacag cagttaatcc tccgaactgc attgttatat tatctgttct caatacattc
    16081 atcatttggc acctcctttt gaaagaggcc tttttgtaaa gaatctgaag aacttgtccc
    16141 agctgaattc cctggttccc atgattccct gcctgaagaa cagaactaca agcattagaa
    16201 gtattgaaaa tacgaccatt ctcaagcccg gcagcgcagg tacattggga aatataggtt
    16261 catccaggaa cctcagaact tcttgtgcta ttgttattat aaaggctgat ataattgtcc
    16321 cggtgatgct tcccattcct cccagcacta ttataagaag tatgttatat gtcatgaaga
    16381 atctgaacat caacgggtca attgttccca tcatattacc cagcaagcct cctcctatgc
    16441 ctgcaaagaa agcacctatg gcaaaggaaa gcaccttatg cttgaacaaa ctgattccca
    16501 tactttctgc agcaatttca tcctccctta ttgccttgaa ggctcttccg aagctgctgt
    16561 taatcagtga taccattata aaaacggtta tcaccgctgt cccaaagctc caccagagat
    16621 ttgtagtagc gggaattccc tttagtccca aagcaccgtt ggttattgtc tgtgtgtttg
    16681 tgaatactac tcttataatc tctgaaaaac ccagagtagc tatggcaaga taatccccct
    16741 taagcctcaa taccggtgct cctataagga aagccacaaa tgcagatagt atacctgcca
    16801 gcaaaagtga cggcagaaaa ggccacacaa ttttgtccaa aggtgaaaca aggggcgcca
    16861 ggaagaaatt catctgcttg gtggcaatag gcaatgtcag cattgccgta gtataagcgc
    16921 ccacggccat aaagcctgcg tgccccaaag agaacaatcc ggtaaaaccg tttattagat
    16981 tcatactaag acccaatacc gtataaatcg cacaaaggtt cagtattctc aaaagatatg
    17041 agtctaaata tttattgccg aaataaacaa atacaaacag tacaattatg gcggtaattg
    17101 tataaatcgt attcctcttc atatttacac cttctccgat attttttctc ccattatgcc
    17161 ggtaggccta aataggagta ttaatataag cagaataaag gagaatgcat ctctgtaacc
    17221 ggaaagctcc gggaggaatg ctaccagaag gatttctcct ataccgagta tgaatccgcc
    17281 aattaccgca ccctgtatat ttccaattcc gcctattact gctgcaataa agcatttaat
    17341 tcccggcatt acgcccatca aaggcaatat gctggggaac ttgaggcccc acattatgcc
    17401 tcctaccgct gccagtaagg atcctatccc aaaggtaaag gatattgtcc tgttcacatc
    17461 aatacccatt atcttcgcag tttcaaaatc ctttgatacg gccctcatgg ccataccgct
    17521 ttttgtcttg ttgataaggt acattaataa catcaggaga accgccgtta ccacagggat
    17581 cataaaagtc agcatctgaa tatggacggt cccgaattgc accatatcgg tgaacaacgg
    17641 gatctgcgga aaggtcttcg gccttccgcc atatatcacg atagccagat tctccaacag
    17701 gtaagaagca cctatggaag aaataagaat tgaaattcgc ggtgcttctc ttatcggcct
    17761 gtaagccagt gtctcaacag taatacccag gactcccgtc agagctattg caataatgaa
    17821 tgacacgtac cacggcattc caaaggtcat aactccaaag aaagcaaagt atactgccat
    17881 catgaatata tcaccgtggg caaaatttat cagcctcaat ataccgtaca ccatcgtgta
    17941 tcctatggca accagtgcat aaaggcttcc cagtgatata ccattagcca aatgctgtaa
    18001 gaacaattcc agtgtcatat atacaccacc taagtaagat ttcgtcaggg aaccttccca
    18061 ttcatttctc attatgccgg aaaacatact cttccgaaca atgtagcatt aattttataa
    18121 cataaaaaaa ttcctggtac cttgtaacat ctaattgttg tattatgata cgcagaatat
    18181 ttgattccaa tacaaggcga tggaaagagg cgcagcagtg gctacggcga ttgacatcaa
    18241 cgcagtagtg gagcaaatag gcaggtatca tgcagcttta ggtgttgaaa ggtactttag
    18301 ttaagtctta tcataaaacg cacttcctcc ctttaaggga ggaaatgcat tttactctac
    18361 tttttatatt tactgatatg gggaaggttt atccatccgt tgatttttat ttagggttca
    18421 actacatcca tgaatgtaaa tttgccgtct tttactgttt ttataacagc gctcttaaca
    18481 gcatctccgt tttcatcaaa gtttacaaca ccagctgcac cctggaaatc ctttatatta
    18541 agcagtgcat tatgaattga tgcggagtcg gctgcccctg cctgttcgat agcctttatg
    18601 agaataaggt atgcatcgta tccaagggct gtaactgctg caggttcttt cccgggatat
    18661 tttgccctgt atgcatccag gaattttgtt gattctgaag ttatcggtct ttctgttgca
    18721 aagaatgttg agaatacaac accttctaca gcatctccgc ctatttcagc aaattccgga
    18781 gtctcataag tatctccacc tataaacgga gtctttattc caagctgtct ggcctgcttt
    18841 ataagaagag ctgattctgt aaagttaccc ggagcgaata taacatcagg attctgtgct
    18901 tttatgtttg taagctgtgc agtgaagtcc tggtctcctg tattatagtt ggcaactgca
    18961 actatactgt tttcatcacc tgtcagcttc ttgaagttat ctgtaaagaa ctttgcaagt
    19021 cctactgcat agtcgttgga tacttcctgc acaatggctg ccttcttggc accaaggcca
    19081 ttgtaagcgt agtttgacat tactactccc tggaagggat ctataaagca aaccctgaag
    19141 taataatcat taccctgtgt aacaagagga ttggtgcaag atgttcccat tgcaggaacc
    19201 tttgcatttt ttacgatatc tcctgctgcc attgcaaggg agcttcccca gcttcctata
    19261 attgcagtaa ctttttcttt ctcaaccaat ctggctgcag ctgtagctgc ttcaaccttg
    19321 tctgatttat tatcaactac tataagctca acctttttgc ccaatacttc gggatataat
    19381 tcgtgggcaa gctcgatacc ttccatttca agctcaccgc cggcagcatt tgcacctgtc
    19441 aaaggttcaa atacaccgat cttaataact tcttcttcag ccggagctgc aggctcaccg
    19501 cttggagccg gagcaggctg tgcacaccct gtaagcaagg atagtgccat cgcaacaact
    19561 aaagtcaagg ataataactt tttcatacta ttcctccttc tcattttcga actgaccatt
    19621 atatagccct tccccatttg agctatcttg ataatgccaa actatccttc tataaccacc
    19681 tccaatattt gatagtgcat caacaacaaa aaatccaatt acagtataac caccacttct
    19741 gaaagtggcg gatttccctc taaaaatatt ataatatatc aaaaatatat ctattcaaat
    19801 aatataataa aatctaatta tagtcaaaca atttgtaaaa tttcatacct ccatccatgc
    19861 gcagaatctg ctttccaatt tgtcaattat cttaaacagc aagagcccca taatacttag
    19921 agccaaaatg ccgctgaaca tctccacata gttgacgaca acccaactgt tcattataaa
    19981 atatcctata ccgtatctgg cggcaaagtt ctcactgaaa aacagcactg caatggaggt
    20041 tcccactgta agccgcaagg ctgttattat tttgggcatt attgccggaa ttatgatgtg
    20101 tctgtacagc tgtgccctgc tcatatctaa ggattcagcg gaaagaaagt attcctttcc
    20161 cagttcatgc actccgtccc tggtggttac aattatttga aatattacta ttattgaaat
    20221 aagaattatc ttggacgcat tccccaatcc gaacaaaatc atcaataccg gcaggaaagc
    20281 aacctttggt attggataaa gtatatatac tatgggtgtt ataagcctgt cggctttatt
    20341 gttcatacct gtaagcagcc ctagcggcaa ccccaggagc agggaaaagg ctgctgcggc
    20401 aattatcctg gccaaactgg ccagtacatg gggcagaagc accgcaggaa gtatcgttat
    20461 aaagttcaat actgcagcaa aggggcttgg tataacaggg gattgaagca taatatacaa
    20521 tatataccag aaaagaaaaa tcataagtgt tccataaagg cttttgcttc ttatcaactt
    20581 attcccccct atccatccat ttgcgcactt ctaggcatat tttgcagtac tccatcctgc
    20641 ttcgaagctc ttcgtcaccg aaataagggt tgtccagcac gtgctttatt tttgccttgc
    20701 ccattactat gattttctgc ccaaggaaaa cagcctcctc aatgctgtga gtcacaatga
    20761 ccatggtcag gggagcactt ttatacagct tcagaataag gttctgtata tgctcctttg
    20821 taatggcatc aagggctgag gaaggttcat ccatcagaag caggtcaggc ttcattgcaa
    20881 gggttctgcc tattgccacc ctctgttttt ctcctccgct cagctctgcg ggatatttac
    20941 agctaagatg tccgattcca agcactgcaa gtatattgct tacggtttca ctgatttttt
    21001 ctttggtttc acctcttacc tgcaggccca gagcaacatt attccagacg tttttccatg
    21061 gaaaaaggcc atagtcctgc agtattattc cggtgcttct tcttatgccg gaaagctctt
    21121 caccgccgat atatattgtt ccctcctccg gacggataag ccccgctaat gtataaagca
    21181 aggtagtctt tccgcatcca gagggcccga ttatcgcaca ggtggcattg cgctccacct
    21241 tcaggctgat tccttccaac gcccgcacat ttccataaga cacctgcagg ttgttaactt
    21301 ctatcattgg ttaatgaact tcccttcaat catatcatca tactttacat tcaccttaat
    21361 attctggatt ttctcaaccc actccacaat cttgcggatg taagcttcat caggaagcct
    21421 ggttttgtga tacacgggga gtattatttt atcctttatt cccggtttaa gctgcagttt
    21481 ttctatcagt acttcccttg caagggtgtc atcccttttt atatcgtcta cggctttgtc
    21541 aaaggctctg tggaacagct caattgcctt ttccttctct ttgagtgctt ttcccgtaaa
    21601 ggccattgcc tcaggcataa agtcatcctt gttctcgtag acaacctttt ttagtccgtt
    21661 aagttctccc atggaggcca taggttccgg aatattggca atgtccaaat tccccgcttt
    21721 tatcatctcc agccttgcgg gaatctcatt gatatatacc ttctccattt catacttgtc
    21781 ccccaagtat tcctccgaca gaaaatttgt aacgctcact tccatcattc ctactttgat
    21841 attcttcttt tcctttaaat ccttgtttac gagaaatggg aagcttccgt cagtacttgt
    21901 ggttattttt atatcaaagc cgttatgtac attattcaca aatgcaatca agtccaccat
    21961 agcaccgtca agctctccgc tctgcagggc actttgcctg ttagccgcat ttgtatatac
    22021 ctgtatttcc gcatccaatc ccagttcctt aaagtatccc ttttcttcag caaggaatat
    22081 cggtgcggag tctgctgcgg gcattacccc gattttcagc ttgggtattt cagttatatc
    22141 aggttcctta ttgtctgccg tactacatcc tgcagcagct attgctgtgc agatcagcat
    22201 gattattact gccttgagct ttttcattca cacaacctcc ggtttttctg atatgtatat
    22261 atattatttc ttattcttcc aaatgacaag aaaaaaagcc gcaaatgcgg ctgatttata
    22321 tgaatttata ctaaaaacac ggcggctatt gtagttatta taagccctat tactacaggc
    22381 ttcagattgc ggcgggccaa ttcgaaggga tcgactccgc atattgctgc cgcagggatt
    22441 accgcccaag gtatcagagt gcctccccct acccagatgg ctgcaacctg gcctaaggct
    22501 gtaagcgtcg ccgctcctgt gcctaaggct atagaaaata gctttgcaat agacccgact
    22561 agggatatgc ctgaaaatcc tgaaccgtca agtcctgtaa ttgcaccgac ggctgtggct
    22621 gtgacagctc cgattccccg gttcagcggc actgcgcctg ccaaggcaac acccaggtca
    22681 tttacaagcc cgtttgagcc ttccggaagc accttgccga atatatcgat tatagccgaa
    22741 tcgcccaagt agaaaaatgc tgcaatagga ataaccggcc cgaacaccct gaagccgaac
    22801 tgcaatcctt ctattaaatg ctctgtaatt ttgcccagag gatctgcact ataagcaaag
    22861 aaggatacaa ttatcaatat cagtactgat gttccgccga ttaaagctgt tgcatccccg
    22921 ccctgaagct ttagaataaa catcatcaat atatctacgg caaacagtac gggtaccatc
    22981 gcagcaagga gtttttttac aacaggaggt atttctttca tacttgcctt accgtcgttg
    23041 atttgcacaa tcatgctttt tctcatgttt tcatcttcaa ccttcaatgc tccggacttc
    23101 aagtcctttc gaagtatcca gaatgcagag atggttgtga ctgcacccat aattatcacc
    23161 aatggcacac tggccgctac cacatcgatt acaggtatcc cggcaccatc ggcagttaat
    23221 ttcggcgctc cctggataat aaagtctcct gataatgcta tgccgtggcc gaaaaggttc
    23281 atagccacag ctacgcccat ggcgggaagc tttgctttaa gtgcgaccgg cagaagtact
    23341 gcccccatca gcgcaacagc aggagagggc cagaagaacc aggaaatgat catcatcagt
    23401 atgcctataa cccagtatgc cagggccgga gtacgtatca gccttgtaaa tgggtatacc
    23461 atagcttcat taatacctgt tttcatcaat actttactca ttccgtaaat aatcgaaata
    23521 atcaatattg tccccaatag ttccttaata gcgtatgcaa aactgttaaa gaccccggtt
    23581 accgaatgag tgattgtgga ggttgctata aaccccagaa cgagaattcc ggcaacacag
    23641 atgaagctgg tatccttcct cattacaatg aaggcaatta ccagcagaat gaagatcaag
    23701 tacacccaat gcagtgttgt aagtgtaatc cccattgagc ctcctcctta taaaagaatg
    23761 taattactac acaatatgca gaggcatcaa taagggagct aaattcttcg caaaataaga
    23821 agaatgcgac ggggaaacgt ccccgcgtcc ccgtcgcatc attcttctat tgttcatatt
    23881 aaaaaagata tgcccggcaa agctgctgaa catatctttt ataccttttt aatagcaata
    23941 gaagctttct atctgcctca ggtcaactcc gaagaatacc cacctgcgtc ccgtccacct
    24001 ccagcctgaa attgagcgtc tacccacaaa tgtcagccac atccagaatt ccctgccatt
    24061 tctgagctgt aagtaaacga aactgaatct gcatcttctt attgacccgg gatctactgc
    24121 cagagtccct ataccctgcg gccccccaaa ctgtggtgtt atagaaggcg gcggccctga
    24181 tggggggcca tccgaagagc ccggccctac acccggcggt tgaaattgcg gtccaaaagg
    24241 cccgaaggtt ggcggccgct gcctgtgaaa cggacagctt tcacagagct catagtccag
    24301 ttcaatatcg tctttttcat catcatacat atatattcac tcccatgcac actttcttac
    24361 attatatgca tggagcaggt ccaagctgca agtccatact tatccggtta ttttctggta
    24421 gccaatatgt tattttcaat ttcatacatc ttattaattt cttcttcgtt taatcttgat
    24481 aatatttctt tcctgtattt agcggctatc agatgcctct cctttatatc cttttcaacc
    24541 gtttcagccc aatacagctt ttttctctta taaaaaaggt catagtctac tattctggtt
    24601 ccatcctcca ggaattccaa acccatcaat aactttttta tatatatcgg gtcataatag
    24661 gtaaaaaacc ttactctgta tatctcaatc gccatcatag gcctcggata gtcttttccc
    24721 caggatgccc ccagtataat tccaatctcc agggattcca ttatattaat gagtcttgtg
    24781 taatggaggt catactcccc atatttgtca tatatcctga tttcaagctc ataccagtcc
    24841 ttattgccta taccgttgtt caggtatttt aatgcccggg atctgaggct tcgtataaga
    24901 ggcttgtatt tttcattaat aagcagaccc ttttcataaa tatccttctt ataacttatg
    24961 tttctatgca ggggtttttc tgtaacaacc accagagtcc ccttctcatc actggattta
    25021 ataatatcca gcgggcttcc cacaatacat tgatagtccc cgcttatatc ccttactacc
    25081 ctttccaggt cccccattgc tcttaatact attatcctcg ttgcaatccg tgacctgctt
    25141 acaagatcaa tctttttatc gttcaacata ttgcatagta ttacatcagg ttcttcatta
    25201 attaaaatcc cgttatctgc ttcatcaaaa tcaactgctt ctttaatttt atcaatgata
    25261 aaaagaattg tatggtcttc ccctgcattt tcaaatacat cacttggatt ataagcttct
    25321 gcgcccttct cttgaataaa gtagttcctt aattcttcta ttctgctgct ttcaagaagt
    25381 aatactttgg gtccccctct aaccaagctg agcataaata accctccctt gatattattt
    25441 tttacagtcc cactttcctt cttcctaccc ttgcgtcatt tgtacataaa ggataatgta
    25501 gagtcgtgaa ctctcctgac tctaacccta taatgtttat atatgttatc ccgttaaaat
    25561 acagtttatc atcaatacat ccattcttaa gcgttaaatc atgtccaaac agattatacc
    25621 gggacgggtt ttcttgaacg cctttaggaa aatggctgat tctaaaactc aatccctcta
    25681 aattaattgt ctcagcttct attattatgc ggcttcttac aaaatgctta ttgacaacat
    25741 caatattgtc atggttgcca attgccaata taacttctgc tccggaagcc tcaagcatgt
    25801 ctgccagaac ctttaccccc ttctcatgct catggatcaa tgcagggtaa agctccagct
    25861 ttatattgtc cgtaaggtcc cccgtatgca ctatatatgc aggcttaagc catccaataa
    25921 gccttttgag ttcagaataa aaacaaagag gggtatcaga aatatgcagc agtttttttt
    25981 cttcggattt caacagctcc gaagaaatat aaagcttttt tattgtatta tatataaatt
    26041 tcttaaagct taacatcata tgctccttat ttcaatgtgt aaataatatt attccatcta
    26101 ttcacgtata agtcaaacaa aaagaatagc ttgtttgcta ttcctaaata tattcttaat
    26161 attaaatctt tggcagattt attccgtaaa aaatctctgt gatttccctg tccagcttct
    26221 tcgatatatc ctttttctcc tttggagtca agtcatcttc ctctatctca aataaatagt
    26281 tgttcaaatc caaatccctt atcctcattt ttgtatggaa tatattggac tggtatatgt
    26341 tcatatcaat aaggtcatat ctgtttattg tgtccttggc aatgaagttt tgtattgagt
    26401 ttatcttatg gtctatgaag tgtttccggc ctcgaatatt tcttgtatat cccctgaccc
    26461 tgtagtcgat agtaattata tccgaatcaa agctcccgat aagataattc aatgccttta
    26521 gtggggaaat agttccacag gtagtcacat ctatatcaac cctgaaggta cttatctcat
    26581 tctgaggatg gctctcgggg tacgtgtgta ctgtcacgtg actcttgtca aggtgtccca
    26641 caatattctc cctgataggg gttattattc ctctgttaca ggaagggtca agcacataaa
    26701 gagggacctc ttcttccgaa ataagcagtg taacacttgc accctgagga tcataatcct
    26761 gtttggctac attcagtaca ctggcaccta tcatttcggt aacatcactg agtatctttg
    26821 taagccttgt agaattatac tgttcatcaa tatattcaat atatttctta cggtcttctc
    26881 tagatttggt atagcaaata tcatagatgt taaaactcaa agatttcgta agattattga
    26941 agccatagag cttcagcttg ccattttcgg ctttcataag catcacaccc catttccatt
    27001 aatcttatta gcatttattt aaaatgcaaa tcataatcat attaagccct aaaaatgcaa
    27061 atgtcaatgt tccttctatt ttgtcagata aaatgtttta cagtaattat ttccccgcct
    27121 tttccagttc ctccaccatt tttatatgtt tcttcggtga aagcccgtac attcttttaa
    27181 aggcccttac gaagcttgaa taatcgccaa agccgcattc cgtacatgct tctgctactg
    27241 attttcctga ctttatgagt gtattggcca tcatcagccg cttttgaagg atgtagctgt
    27301 gaatggtata acctgtctgg cttttaaact tatgcatcaa atgatacttg ctcatataga
    27361 atttggatgc caggccttct attggcatat cctcacaaat attctcgttt atatgcctta
    27421 ttacttctcc tatgctttcg tcataatccc cgtcagcctt atgctctatg gaatcaggct
    27481 caagataaat tctattaaga tatactatga attgaaggaa taatgagttc ttaagcacct
    27541 gacttccaaa accgccgctc ttgcattcct ccttcagctg agaaagaata tgtttcccct
    27601 gcctgagaag cttaggattc agcctcagta tattgcgctt ctcttttgag gcaatatcaa
    27661 agcatctcaa caaatcaccc tcaggacttt tatacttatg cagaaaattt gaattgaccc
    27721 atattatcat cctctcataa gtcccgctag tgcctactat tgctttatgt atctcgctgc
    27781 tgccgataag aagcatatcc cagggtttaa gcttgtacgc cctgccttct atcagatacg
    27841 taacatttcc cgacatgaag atgattattt tgttaaaatc atgaaagtgc aattcaaact
    27901 ccataatttt cctgtctttt aaatgaaaaa actcaaaatc cttatttaaa tatccccttc
    27961 tgctgcccga aacaactgcc tgatccttca tgctttacct ccaattcgtt accgaagttg
    28021 ctgagacttt cgttctgcat acccattgcc tcaataatat cgcaggtttt gcaagttatc
    28081 aagaattata atcaatattt atagcaaaaa ttttgaatat agcgaggcac agttcatact
    28141 attttttgag aagagcgggt tttaacatga tatcaccggc ggctgccgat gagcatccaa
    28201 gtttatgtaa aatctcttct ccgtggtaaa agctgaacgt ttcgtagggt gtacggttgt
    28261 tcagctttgt gagcagcgat atttgatgaa cagattggct ttctgagcaa ggggcgatat
    28321 aatagtattc agccttgcaa tctcgtcctc tgatacacaa aggccactta gggattcgga
    28381 tgctgtttta tgtgccttga tatgcgcgtt ctctgcagga tgactgcaaa cctcagatat
    28441 gtcgcattat tctactattg tgcaatagcc ctttttgcta tataagtcct tcaataatga
    28501 tggtagttcc tattgccagc acttgtacca catttataat cagaaagttc ttaacggaca
    28561 atataaaggt gtccttcttg gtctgctttt caaaaaaact ttttatattg ctgcttacgg
    28621 gaaaaattgt aaaaagcgta atgactgttg ccgcgggggt aactcccaga acaagcagca
    28681 gcagcaaatc tgcatagctg atgtagtata aggctttaaa tacccataaa gccctttcct
    28741 ttcctatata tatgggcaga gtataacgtc tgttctcgat atcgtcttca atatcgcata
    28801 tgttgtttgc cagcatgata ttggcaatgc cgtttactgc aggtaatgac agaagaaata
    28861 tatatattcc ttcagtcagg ttgaagctta ttcccaccat gctgccggaa tatgtcagac
    28921 cagcaatacc cgtgtcatat acatgtacat atattgatat gaaagtaatt acaaacccca
    28981 tgaacaaccc ggaaaacacc tctccaaagg gtgtccggga aatcggtatc ggtccgaagg
    29041 aatacagaat tcctactgca aaggacaaaa cccccagcag cagaataacg ggagatgtat
    29101 taagaaaaag cataaggccg aaaacagatg caacagtcag caaagcggtt attgttagtt
    29161 ttacagttga ttctttcaaa ttgtatttta ctatggcatt atgactttcg taattaaagc
    29221 cctgggtctt gttcgctctc ttgtaatcat agtaattgtt aattgctgta gttgccatat
    29281 caaaggatag caatgatatg aacattatta tgaagttcct gggattaaag attccaaagt
    29341 gataaactgt atataaggtt cccaatgcaa agggtattac gcttgcaact tttgtctgta
    29401 tttcaaccag tttgagaaaa ctctttaaag acattgcaat tctccttttt gacagtataa
    29461 ctattggtct tctgcttttc tgtcttcctt tttgccgcga accctgtcgt acaagaattt
    29521 taaatcctca tcaatgaatc tgtgcttagt ctccttcagg ttatatttga aaagcttaaa
    29581 ggaaaactga acaaaatatc cggctgtaat tgccataatt gcagttccta ttcccacaga
    29641 acctccaagg atatatcctg cggccagtgc ggttatttcg atgctgtttc ttatgaacct
    29701 gacggacttg ccggtctttt ttgtcagcgc caccatcaga ccgtcccttg ggccggagcc
    29761 caatccgacg cttatgtaga aatagctggc tattccgata acgaacatgc ccagaagcat
    29821 cattgcaaat cttagtatta ctgactgaaa taccggaatc aggtgattca gcattagtat
    29881 atccatgaac aatccaatga atagcatgtt gaacagtgtt ccccatccga gcctttctcc
    29941 aagcagactg tcaagtccta caaggaccat cccgacatat atgcttgcct gacccattgt
    30001 aattccggtt tttattgaca atccctgatg aaatatctcc caaggtgcca aacccagatt
    30061 ggcattgatt gtcataacaa tccccaaacc atacaaaaac aacccaaaga acaattttgc
    30121 catacgtttt atgatttctt tcatttctgt ctatctcctt ctacgattcg ctttccctta
    30181 aatccctccg gattattctt atgccacttc catgcagtcg ctataatttc atcgatgcta
    30241 ttgtacctgg gctgccatcc catctcatcc ccggcctttt ttgcggaggc tatcagtgtg
    30301 gaagggtctc ctgcccttct ggggcatatt actgccggta tcgggtgatt ggtcaccctt
    30361 cttgctgcct cgacaatttc cttaactgag aagccttcac cgtttccgag gttataaaca
    30421 gtgcttgcag ctccgtctct caagcggtca agagccatga tatgcgcatt tgtcaaatcc
    30481 gttacatgta tataatccct tatacaggta ccgtcataag tatcataatc atcaccgaat
    30541 attgagatgc tttcccgctt cccaagggga acctgaagta taagcggaat cagatgtgtc
    30601 tcagggcagt ggtcctcgcc gattcttccg ctttcatggg ctccggctac gttaaagtac
    30661 ctgagggcta tatactttat accatgggca atatccgccc acttcatcat tttttccatt
    30721 acaagcttgg tttccccata ggcattagtt ggttcagtcc tgtccgtctc cagaatggga
    30781 atgctctccg gctcgccgta ggcagctgca gaggatgaaa atactattct gttcacatta
    30841 tgcctgttca ttgctgaaag cagtacttct gttccatata cattattgtc aaagtaagaa
    30901 agaggctctt tcatgctcac gccaaccagg gaatcggcag caaaatggat taccgtatca
    30961 atatcattct cattaaatat cttatcgaga aatttcccat cccggatatc ccctttgtaa
    31021 aatcttgctt ctccatgcac agcaccctcg tggcctgttt gcaggttatc tgcaacaaca
    31081 actttttctt ttctgtcaat aaggctgcag acgcagtggg aaccaatata tcctgcgcct
    31141 cccgttacca agactgccaa ttattattcc ctccttcaca gggctgttta tttccatgct
    31201 gattggtctc gtcctttaat attataatat aatttcccgg gtattactat tatagaatca
    31261 agaacatggg acacggggac gtttctcctg tcccgctttc aggggacagg agaaacgtcc
    31321 ccgtgtccca cgtccccgtg tcctccatgc atttgctttt caacataggc tgttttataa
    31381 tttatttagc attcaaaggg aggaatggaa atgcagtttg atgcccataa atataattac
    31441 ccttcaagaa gaaatattat atatgcaaga aaaggcatga tcgccgcctc acatcccctt
    31501 gcagcacaag ccggcctgga aatgctcaaa aaaggaggca atgccattga tgccgcagtt
    31561 gcgacggctg cctgccttac tgtagtggag cccacatcca acggtatcgg aggagatgcc
    31621 tttgcccttg tatggtcaga aggaaaactc catgggctga atgccagcgg gcctgcaccg
    31681 gcttccattc ctgccgaaat aatcgaaaaa atccaaagcc gcggcattcc caagcatggc
    31741 tggatgcctg tcaccgttcc cggtataccc tccgcatggt cggcgctgtc aaaacgtttt
    31801 ggcaggcttc ctctttcaga agccctatct cctgcagttg cttatgctta tgaagggcat
    31861 cctgtcgcac ctaccgtcgc aaaatcctgg gaaagcgcat acaggaccta tagaatagcc
    31921 ctaaaggacg aattattccg gcactggttc gatacctttg ccccatcagg cagagcgcca
    31981 aaggccgggg agctgtggag ttcacccgcc catggaaaaa cccttcagga gataggtgaa
    32041 acaaatgccg aaagcttcta tcggggagcc ttggcagaaa aaatttccga atttgccgga
    32101 aaaaccggag gatatttgtc cctgaaggat cttgagtctt ttgaaccgga atgggttgaa
    32161 cccataagta ttaactatcg agggtatgac atatgggaaa tccccccaaa cgggcacgga
    32221 atagtggctt taatggcttt gaatatacta aagggatttg attttgaact aagggattcc
    32281 attgatactt atcatcatca gttggaagcg atgaagcttg catttgccga cggaataagg
    32341 tatatagctg atcgcagaag tatgaaggta tccactgagg aactgctctc tgataattat
    32401 gctgccgaaa gaaggaagtg tatcggcagc aaagcgctga tacccacgcc gggcaggcct
    32461 cccgaagggg gcacggtata cctggcggca gccgatggag aaggcaatat ggtatcgtat
    32521 atccaaagca actatatggg ctttggctcg ggtattgtca tacccgggac aggtattagc
    32581 cttcataaca gaggctgcaa tttctcaact gatccgtccc gtgaaaactg cattgccccg
    32641 gggaagaagc cttatcatac aattattccc ggattcctta cccgggaagg cagagctatc
    32701 ggccctttcg gagtaatggg tgcatttatg cagcctcaag gccatgtcca ggtgattatg
    32761 aataccttag acttccatat gaatccccag gattgcctgg attcacccag atggcagtgg
    32821 attgcggaaa aggatattga agtggaagaa ggtttcccct tagatgctgt acagggcctt
    32881 attaataaag gacataatat aaaaattaat ccttattccg ggagctttgg aagaggacag
    32941 atcatatggc gggacgagag aaatattttg gttggcggca ctgagccaag gacagacggc
    33001 gcaatcgccg cctggtaatc cgctacatca gaattttgcg gcgtatcagg tagtcgtgaa
    33061 gcttttcaaa tatgagccct ccaaaaaacc aaaccgggat aaaggataag gtgattatcc
    33121 cgtcgataga atatggccat gcactgtaat cccatggaca ctttcctata atctctctga
    33181 gaatccatcc ggttgtgtat tctattccaa atatgattat agtgtacaca ccccctcttg
    33241 ccacaaccgg agcgtttctt atattatcat ggacaggctc taaaagtatc ataagcccat
    33301 atatcggaaa catccatata taagtccatg tatgcattgt catatcgccc ttgaacaggg
    33361 atcctaatcc cgtaaatatt gtctccgcta tccatcctgc cagtccatat ataacaaatc
    33421 tttttttcat acagatattt tctgcatgta tataaaaata attcgttatt tcaattcatc
    33481 caattccggc tgatcaaata tgagcttctc cccattatca agcattataa agggtattcc
    33541 cactctgccg attctcttga tttcttcaaa ctcaggcctt gtatcccgca gcttgagata
    33601 gactttcagg ttcttcatac tgtctgttat atcaatatat tcgtagataa tcccattatt
    33661 tgaaagaaac tctttcaatg gctggcaatc cggtcaatac ctgctgccga atacagttat
    33721 ttttttcatt ttgtattcct ccttgttgtt caatattctt attataagtt ataacaaggc
    33781 gaaaagatat tgtctaacag gcagtgagca tatctctcgc cttgttgaaa cgctcgggta
    33841 ggtttttgca tgtatgcaaa aaccctcatt aaaatcttgt tataagcata ttttatcaaa
    33901 tattaaatac tataatactg ttgccataat ctttaagaaa ggaagatgca tattggaatt
    33961 aaaagagcct cttgtatgca caaaatgcag cggcagccat tttaccgtaa aacgtgaagc
    34021 cacctattta tacagctatg accttgagaa caaaaaatcc gaagcactgc cttttttatt
    34081 cgataacaga gaactgataa aaagccatga atatctgcag tgtgagcagt gcggtgcaaa
    34141 attcccctgt actctggatg agcgcagcaa tacaataaat ttcaccatag agcaaaaagc
    34201 cgtaagatcg gaccatgtaa caaatccgga atttctggga taacccttct cccataaagt
    34261 taaataaata tcaatgggtt gtaaatattt gctatgcatt gtattatatt agtaattaaa
    34321 taatgctgaa ccagatgaaa atggcgagag ataccttgta aggtagccga agaagcaatg
    34381 tgaagagcag cccctcttca cagacacttt caggcaaaag gatcgccacg ggatggtact
    34441 ctgaagagac cgtttatcgg cgccgtagga gaaatacata gatatgtaga agctctcagg
    34501 caaaagaaca gagggatata atggcacata aatttattgt gcaattatat ccctttttaa
    34561 tttggataac atattactaa gttactaaaa ggaggattat tttatgagcg aacctaagaa
    34621 aacgtccctt tacgatgtcc acgaaaaata cggcggaaga gtaattgatt ttgccggatg
    34681 ggcattgccg gtacagtacg aaggcataac agcagagcat gaagcagtga ggaattgnnn
    34741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc agggttgttg acagaagatt
    34861 ctatactaaa aagtacagaa aataatataa aaatatattt atcaggagga atgaaaaatg
    34921 gaaacaaaat caggattgct ttattcaaag gatcacgaat gggtcagggt tgaaggcgaa
    34981 aaggcctatg tgggcatcac cgattatgcc cagcatgctt tgggggaaat agtctatgtg
    35041 gaattgccgg cagtagggga cgaactcaat gccggggatg ttttcagcgt tattgaatct
    35101 gtaaaggcag cttctgactc ataccttcca attgccggaa aagttcttga agtaaatgca
    35161 gcattgtcgg attcaccgca gcttgtaaac gaggatccct atggaagctg gattgtagtg
    35221 gttgagataa ctgacaaggc cggccttgat gaactcatga gcgagcagga gtaccgggaa
    35281 ttctgcgata aggaggcata atatgcatag gtatttacct cataccgagg atgatgaaag
    35341 actgatgctt gaaagtatcg gagcaaaatc aacagatgat ttatttgtag atataccccg
    35401 ggaactgcgg cttggaagaa agcttaatct gaacaatcct ctttcggaaa ttgagctttc
    35461 agaccatatg aagggtctga gcaataaaaa caagagttcg gatgatctgg tctgctttct
    35521 gggagcagga gcatacgacc attttattcc ctctgtcgta aagcatcttg caatgaggtc
    35581 ggaattctat accgcatata ctccttacca gcctgagata agccagggaa cactccaggc
    35641 aatatttgag tatcagacta tgatttgcag cctcacggga atggatgtta ccaatgcctc
    35701 catgtatgac ggagctacag cctgtgttga agcggcaatg atggctgtcg aaaatgcaag
    35761 gcgcagctcg ataatagtgt caagaaccgt gcaccctgaa gtaagaaaag tattgaaaag
    35821 ctatatgagg ttccgggatg tggaagtcat tgaagccgat atggccgatg gcataactga
    35881 tgttgaaaag ctgaagcccc ttgtggaagc aggcaccgcc ggtgtcatag tccaaaatcc
    35941 gaatttcttc ggtataatag aggatttgac cgaaatagaa aaaatggtgc atgcaaataa
    36001 agcactgctt attgactacg cggaccccat ttctctcgga atattgaaaa gccccggtga
    36061 gtcaggggtg gatatagttg cgggggaagg ccagtctctt gggaacagcc tcagcttcgg
    36121 aggcccttac ctggggtttc tcgccacaac ctccaagctg atgagaaaaa tgcccggaag
    36181 aattgtaggg caatcggaag acgcagacga caagagggct tttgtactta ccctgcaggc
    36241 cagggaacag catatccgca gatacaaggc aacctccaac atatgctcca accagggcct
    36301 taatgctctt gccgcagcaa tttacatgac tacgatgggc aaaaagggcc ttaaggaagc
    36361 agcggaacaa agcgcaaaaa aggcccacta tgccatgaag cagctgacaa gcagcggaaa
    36421 gttcaagcct ctgtttaaca agcccttctt caaggaattt gccgttacgg gtgaaacacc
    36481 cgccggtaag attaatgacg agctgctgaa gcataatata cttggaggct ttgagctgga
    36541 taaggaatac cctgagctga agaacggctt gcttttctgt gtgactgaaa aacggacaaa
    36601 agctcagatt gacaaactgg cacaagtaat ggaggtggta tgatgaaaaa ttacaacagc
    36661 cttatttttg agctttccaa agaaggaaga aaagcataca gcctgccgcc ttgcgacgtt
    36721 tctgacatca gccttgacag tgtgattccc ccttcgctta tgagggatag tgaacctgag
    36781 ctgcctgagg tcagtgaagt agatgtggta agacacttta caaatctctc aaataaaaat
    36841 tatggagtgg ataccggctt ttacccgctg ggctcctgta ccatgaagta taatccaaag
    36901 ataaatgaag acatggctaa tctttcggga ttcagcaata ttcatcccct ccagcctgaa
    36961 gctactgtcc agggagccct gaagctgatg tacgagtttg acggtatgct ttcagaaatc
    37021 agcggtatgg acagggtttc cctccagccc gccgccggtg ctcacggtga gcttaccggc
    37081 cttatgatta tcaaggcata tcatgagagc cggggcgaca agaaaagaaa aaagataata
    37141 gtgccggact ctgcccacgg aacaaaccct gccagcgcag ctgtcgcagg ctttgaaata
    37201 gtggaaattg aatctgacaa aaacggagct gttgatcttg ataggctgaa ggcagtgctg
    37261 agtgatgaaa ttgccggcct aatgctgaca aatccaagca cactcgggct tttcgagaaa
    37321 aatatcaagc aaatcgcaaa cctggttcat gaggcaggag gactccttta ctacgacgga
    37381 gccaatgcca acgccattat gggaattgca aggcccgggg atatgggttt tgatgtgatc
    37441 catctcaacc tgcataagac attttccaca cctcacggcg ggggcggccc gggaagcggg
    37501 ccggtaggag tcaaaagtga acttgccagg ttcctccctg ttcctgttat tgagaaaaag
    37561 ggtgattcct atatactgga ctatgacagg cctgactcca taggcaaggt aaagagcttc
    37621 tacggcaact tcggagtcgc cgtaaaggct tatgcctaca tactgtcaat ggggggggaa
    37681 ggcttaacgg atgtaagccg gaaggcggtt cttaatgcaa actatatgat gagtaaactg
    37741 aagaaatact atgagcttcc gatagatcag gtgtgcaagc atgagttcgt tttgagcggg
    37801 cttctggaaa acgacaatca tattgcgact ctggatgttg caaagcgtct tctggactat
    37861 ggctatcacc ccccgaccat ttacttcccg cttatagtaa ataatgcgat aatgatagag
    37921 cctaccgaaa ctgaaagcct ggagaccctt gacagcttca tagatgtaat gataaaaatc
    37981 gccgaagaag caaaggaagc tcccgatata ttaaaaaccg ctccccatgg cactgtagtc
    38041 aggaggatag acgaagcaaa agcggcaaga agccttatcc tcaagtggga cggtacttgc
    38101 atgaacaagg agtgatttat atgaatccgt ttataaaaaa actggctgac tcgaagttca
    38161 tgaccaaagc tcaaggaaat tacattgaag aggctgtaat gagaaaggac tccattatta
    38221 tttccgggca caagggattc ggaatcctgc ctctttttgc aactataagc gcaacagcaa
    38281 aaagcaattt caagataaag cagataaagg gctttgaaga ccttggagat gaagctgact
    38341 actacctgat tgcagacctg agcaatattg actatgcaaa gcttattgca gaagcgatgc
    38401 tgaagcaggg ctcatccttc atcgccctca aggatcccga ccatccctat tcagtcttca
    38461 agatactggg tgatgtctat aagatcaacg gggatacatc aaaggtatat cagcttattg
    38521 aatgtgtaaa gattgacggt gttcctcagc tttccaagat tactcggatt accttgaacg
    38581 aaaacggaaa accggtaaaa accgactttt aagaataatg cgacgcgaga caggggaaac
    38641 gtccccatgt cccgtgtcgc attattcttc tattttgcaa ttgccccttg cttcagagaa
    38701 attagctgct tgacatcttc aagctgcaaa tcattcttta cttgaagcat caaccacttg
    38761 gttccattga acggctttgc cgatcttata gtttgacggg tttcttcagt aagctctccc
    38821 gataaggctt caattacagg cataaggtca agggtgacga caaccaaggc aacaaaaccg
    38881 tcctgctccg gatataaagt gcagatggac ttcccggatt tctgatattt tacattccat
    38941 ccgggttttc ctgagcatac gctgtacatt atcctgggta aggatttata tctctgctgg
    39001 ataaaaccat tgatctgcct ccataaatc
//
LOCUS       DAOQUR010000020        36042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1360_length_36042_cov_10.896463, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000020 DAOQUR010000000
VERSION     DAOQUR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 36042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36042
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1360_length_36042_cov_10.896463"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKI50_06065"
     CDS             complement(<1..457)
                     /locus_tag="PKI50_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNT03311.1"
                     /translation="MEKTIPVEKNRNYIVDITGLTSEGLGVAKIEGFTVFVEGALPKE
                     QSEIRIVKVLKSYAFGKLLKTLKAAACRIEPSCGVVKRCGGCQLQHMSYEAQLRYKTL
                     QVKDALERIGGFKGVSVNDAIGMKDSWRYRNKAQFPVGMDRDVKIGFYAN"
     gene            complement(411..989)
                     /locus_tag="PKI50_06070"
     CDS             complement(411..989)
                     /locus_tag="PKI50_06070"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNT03312.1"
                     /translation="MDKNDLKDIIISDEEEREEQIHELIRGKRSLDINKELSKTLTFG
                     ERMADKIARFAGSWSFLGFFAIVVLAWAFINSKIVLDKPFDRYPYVFLNLVLGCIASI
                     QAPIIMMSQNREAQKDRLHSENEYLINLKSEIILEDLHMKTDQIIREQRLLKKELENI
                     KMEMEKTSSNIQLGRKVDGKNDTGGKEPELYS"
     gene            complement(1057..1266)
                     /locus_tag="PKI50_06075"
     CDS             complement(1057..1266)
                     /locus_tag="PKI50_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03313.1"
                     /translation="MASRLSIKLVIGSEDRKILKRLENTIIKLIKGNTYENKEYIKEL
                     KVCLGNIQLCLRELKTNLKDVRNVQ"
     gene            complement(1530..2828)
                     /locus_tag="PKI50_06080"
     CDS             complement(1530..2828)
                     /locus_tag="PKI50_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNT03314.1"
                     /translation="MSKYFTYEERLTLQKYLKESLSFKEIARRLDKSPTTISREVRKY
                     LSEVATGYPGFSYNACKNRFNCRKKGICGKECTRTSSVYCKLCPRCNDNCPDFIEEIC
                     TARFRVPYICNGCEQINKCTLMKNLYDAEHAHIKAHETVSESRSGLCICEKEIARLNA
                     IISPLVLQGQSIHQIYVSHEDELMCSEKTIYNYIDACLLDVRNIDLPRKVKFRERYKK
                     PEFKVDKGCRIGRNYEAFQSFIDKNPDTTIVQMDSVIGSKGGKCMLTIHFVETNLMLA
                     YLRESNTSQSVIDIFNDLDQKLERELFRKLFPVILTDNGSEFSNPKAIEYGPYQNGTL
                     RTYIYYCDAGSPHQKGAIEVNHSLIRRVLPKGTCFNNLEQEDITRMMNHINSYKRKKL
                     NNRSPYETFSFYHGEEVLHKLGCSPVAADDILLKPTLLKR"
     gene            complement(2972..3931)
                     /locus_tag="PKI50_06085"
     CDS             complement(2972..3931)
                     /locus_tag="PKI50_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT03315.1"
                     /translation="MKLKADDIAGISGHQQIAYNVELITKRIREYYLEEDINALEKLR
                     DLCKNEGMMIEAAKMHRNIGMYHFYMNNCSNAVKSMKLAIDTVRREDCLNLLVEYYSE
                     LGYMYFYNHEYIRSVEYYEKAEELLLQVSEMGREISYLHYYRYGILLSNMQDYYYSEQ
                     KLKNALLYSGDDKDTALIIMNIGLLFKRQKDFKAALRYYSKALCLADQKDMRVKSKVY
                     NNIAIVYKILGQYKKALSYIDKAFKCIEDDDMAMRFICFNTYTEIKLLMGEKESVLDD
                     FLELLLKVEDIHLYKGYIIQGISNMIVIGSVNCKSKCNPIDSN"
     gene            complement(4150..5157)
                     /locus_tag="PKI50_06090"
     CDS             complement(4150..5157)
                     /locus_tag="PKI50_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT03316.1"
                     /translation="MQYRMLGKTGMKVSGISLGGIPLQQLNQDDANEFIEKALEQGIN
                     FIDTARGYTVSESLIGGALSGKRQQVFLATKSMARDREGFKKDIEISLNNFKTDYIDL
                     YQFHNIVSLQELEKAMAKGGAIEAMLEYKDKGIIKSIGITGHSLEVIEKAIEYDVFET
                     IQFPYNAVETQGTELFRKAYEKNLGTIAMKPFAGGAITNARASLKFILENQHITCAIP
                     GMFSIEQLMDNIKAIEEMPLSQDEKEALSKEASELGERFCRRCGYCQPCPKGINIPVV
                     LVMESYYKRYNLMEWAQTRYNNMPSRASDCEKCGLCEAKCPYQLPIREMLEAVKQTFG
                     Q"
     gene            complement(5161..5586)
                     /locus_tag="PKI50_06095"
     CDS             complement(5161..5586)
                     /locus_tag="PKI50_06095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT03317.1"
                     /translation="MHETRLRARYGETDQMGIIYHPNYYIYFEMGRTEYLREAGGISY
                     KEMEELGIMLPLTETHCKYRIPAVYDDEILVRTGIKEMTVARIAFSYKLLRTSDGALL
                     AEGETVHAFANRSGRPINIKKHCSNLYSSLYELSKDKEE"
     gene            complement(5721..7466)
                     /gene="pyk"
                     /locus_tag="PKI50_06100"
     CDS             complement(5721..7466)
                     /gene="pyk"
                     /locus_tag="PKI50_06100"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNT03318.1"
                     /translation="MRKTKIVCTIGPATESEEMISRLITAGMNVARLNFSHGDHAEHK
                     RRIDVIKRIREKLGIPVAIMLDTKGPEVRLGYFKDGKAELEKGQDFTLTIRDIQGDGT
                     QCSITYKELPDEVAVGSRILIADGVIELRVLDKNETDVICRVVNGGTLGDRKNVNIPG
                     ATSKLPAITDKDVADLVFGIENEVDFIAASFIRKAADVLEIRKILEENGGGHIQIIAK
                     IENQEGVDNVKDILRVCDGLMVARGDLGVEIPTEEIPLTQKLLIKRSNEAGKPVITAT
                     QMLDSMMRNPRPTRAEVTDVANSIIDGTDAIMLSGETAAGKYPVETVLTMSSIAERIE
                     RELDYGKSLSSFSGRRITIANAISHSSCTTAFELQAAAIITPTQSGSTARMVSKFRPK
                     APIIATTTDAGVMRKLCLSFGVYPIYVPFTERTDDIIDRAVDAAVESKLIKNGDLVVI
                     TAGIGAAGSTNLIKVHTVGEILSKGIGIGDRSVTGKVSIVDDIPGDFGKLREGDILVA
                     RSTDIEMIPLMEKASAFIVEEGGITSHAAIAGINLGKPVIVGVENAVSILRNGQLITM
                     DTARGLIYRGAARVL"
     gene            complement(7468..8739)
                     /locus_tag="PKI50_06105"
     CDS             complement(7468..8739)
                     /locus_tag="PKI50_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNT03319.1"
                     /translation="MAALKGNCLVAQSGGPTSVINASACGVIQEAFRSGEIDKVYGAL
                     HGVAGLLKEDLFLLNEESPEEIELMKVTPASALGSCRYKLKSIEASEGDYRRIIDVFQ
                     AHNIRYFFYIGGNDSMDTANKVSQYAINVGYELKSIGVPKTIDNDLPITDHTPGFGSA
                     AKFIATSIKEVGLDARVYDYPTVTICELMGRNAGWLTASAALAKEVEDDAPHFIYLPE
                     VPFSFEKFGKDVKSALDKYNYAVIAFSEGIKTEDGKYISEIEDPNARRDAFGHVQLGG
                     ASEVLERYVLENVCKKVRTVNYSTIQRSAAHWASLTDNEESYMCGKAAVEYAIEGHTG
                     KMVGMVRGKGKEYTCETQLIDLDDVANKEKKIPPEWINKEGNYIEQPLLDYIRPLVIG
                     EVKIRMENGIPRYARLNKKLIEKKLEKWEKK"
     gene            complement(8798..9754)
                     /gene="pfkA"
                     /locus_tag="PKI50_06110"
     CDS             complement(8798..9754)
                     /gene="pfkA"
                     /locus_tag="PKI50_06110"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNT03320.1"
                     /translation="MKNIAVLTSGGDAPGMNAAIRAVVRMGIISGFNVFGVRKGFRGL
                     INGDFEKMNESSVSDIIQRGGTILQTARSEEFKTEEGQDKAAKMAKIFDIDGLVVIGG
                     DGSFAGAKRLSEKGVACIGVPGTIDNDLAYTDYSIGFDTAVNNVLDAINRIRDTSSSH
                     ERASIIEVMGRYSGDIALYAGLAGGAEGIIVPEMGFELDDICRLLIERKNKGKLHSII
                     ILAEGAGDAKQLEQQIMENVDIEVRSTILGYIQRGGSTTAFDRILASRLGARAVELLA
                     EGKSSRVVGIRDNKLIDMEIGEALSMKKVIPRELYELAIKLQ"
     gene            complement(9839..10036)
                     /locus_tag="PKI50_06115"
     CDS             complement(9839..10036)
                     /locus_tag="PKI50_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03321.1"
                     /translation="MWTVVYLAKNKKIAEKISRLMTNEGVLVKLQPVNKNIGEEDSYV
                     EILVLESEVEEAHNILYELGY"
     gene            complement(10178..13663)
                     /locus_tag="PKI50_06120"
     CDS             complement(10178..13663)
                     /locus_tag="PKI50_06120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT03322.1"
                     /translation="MKNFVHLHVHTEYSLLDGAGRVEKLVARAKELGMESIAVTDHGV
                     MYGVMEFYKQAKKYGIKPLIGCEVYVAARTMCDRDPKLDGSQYHLVLLAKNEEGYNNL
                     IKLVSLGFTEGFYYKPRVDMEVLRKYSKGLIALSACLAGAIPGHIIDGNYEAAKRAAL
                     SYNEIFGQGNFYLELQDHGIKEQSLVNQEVVRMSRETGIPLIATNDVHYVDKEDAAAQ
                     DILLCIQTGKTILEEDRLKFQGQEFYLKSSEEMYELFKYVPDALENTAKIAEMCSLEF
                     NFGQVHLPAFDVPEGYTSDTYIRKLCYDGLAKRYAELTDELKERAEYELSVITRMGYS
                     DYYLIVWDYVKFARDNGIMVGPGRGSGAGSLVAYCLGITNIDPIKYNLIFERFLNPER
                     ISMPDFDVDFCYERRQEVIDYVVSKYGKDRVAQIITFGTMAARAAIRDVGRALNIPYA
                     QVDMIAKKIPFEIGMTIEKALKSNPELKELYLGDETARQLIDMSKAVEGMPRHSSTHA
                     AGVVISRKAVTEYVPLQMNEDVITTQFTMGTLEELGLLKMDFLGLRTLTVIRDTLELI
                     KAKGMEAPDMDSMDYDDPNVYKMISQGETYGIFQLESGGMTQCFKELKPNCLEDIIAG
                     ISLYRPGAMDQIPKYIRNKHNPDKIRYMHPALEHILDVTYGCIVYQEQVMQIVRDLGG
                     YSMGRSDLVRRAMGKKKADVMAKERENFINGIVDEAGNLVVKGCVRNGIPADTAADIF
                     DEVAKFAGYGFNKSHAAAYAIIAYQTAWLKYYYPVEFSAALITSVMGNSKKVAEYIQH
                     CKSKGIEVLPPDVNESSVSFTVKENKIRFGLAAVKNVGINAVLSIIKARQEKGRFDGL
                     YDFLQKMDYSIINKRTVESLIKCGAFDSFGVYRSRMLAVYEKLMESIQGQKKNNVEGQ
                     ISLFDTIDDSENIIGNIYPDINEYPKKNMLSMEKEMLGLYISGHPLEEYKSELQASVT
                     ITTAELTPENEEEAQDIERNSELDGKRVIMGGIIVSVKQKTTKTNNMMAFVELEDLYG
                     TLEIIVFPKIYDRFKSLLVQDTIVLVEGRISQKEEEAAKIICDAVKPLKKYAGRKLYI
                     RINTGQQPDIIEQLKKVLIEYKGTQPVILVDKANKDNGKSRVMKADSSIWVDINDKLL
                     NELKEVAGSDCVAVK"
     gene            complement(13695..15038)
                     /locus_tag="PKI50_06125"
     CDS             complement(13695..15038)
                     /locus_tag="PKI50_06125"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNT03323.1"
                     /translation="MKKLEFDCSNSMIRDHELSCLREYVKSAHYMLHEKTGAGNDFLG
                     WVDYPKNYDRDEFERIIKAAGRIKDSSEVLLVIGIGGSYLGARTAVELLGHSFRNLLP
                     KDKRNAPEIYFLGNNISGTYFKELMEVIEGKSVSACVISKSGTTTEPAIAFRFVKSYL
                     EAKYGKQEAAKRIYAVTDASRGALKRLADAEGYESFVIADDIGGRYSVLTPVGLLPIA
                     AAGIDIRSILEGARDAWDEYNEPELDKNQCYRYAAVRSILAGKGKAIEVLINYEPSLQ
                     FFAEWWKQLFGESEGKDGKGIFPASMSFSADLHSLGQFLQDGSRNMFETILNIEKPRF
                     EVEIINDEENLDGLNFISGKTMDFVNKKAFQGTMLAHMDGGVPINVLNIPEISEYYFG
                     EMVYFFEKACGISGYLLGVNPFDQPGVEEYKKNMFALLGKPGYEELKEKLDKKLR"
     gene            complement(15053..16009)
                     /locus_tag="PKI50_06130"
     CDS             complement(15053..16009)
                     /locus_tag="PKI50_06130"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HNT03324.1"
                     /translation="MLVGIDLGGTEIKAGLVDREGRILLQSSIPTNAGRDYRLIIKDM
                     QEQVEKLLKDYGAGIGDMESIGIGAPGLMNEKSGYVIYCTNLFWENVPLGIMLKEHFN
                     KPVYMGNDANAAALAESLFGSTKGVENSVLITIGTGVGSGIIIGHKIYSGSHFAGSEL
                     GHMIVGSNFYRCNCGNMGCLETFASATAMIRYAVNRLERDKIKSSILDKAGDTENIDA
                     RLIFDAAKEGDQLGIETVGRMIKYLSIGIINIFNILDPDIVAIGGGVSKAGAYLTDKL
                     KKEVGGMLFTSGIKYGDIVLAQLGNEAGLLGAAFLGINRNCV"
     gene            complement(16085..17152)
                     /locus_tag="PKI50_06135"
     CDS             complement(16085..17152)
                     /locus_tag="PKI50_06135"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNT03325.1"
                     /translation="MRKLPVDKVKEGMVLARTLYSNDGNILLNAGIILKNSYIAKFRD
                     LGIWEVYIDDEISRDIVLKDVISEETRFEARINIKNAMDSLRYGYSLEVKPIRNSVCK
                     MIDELMDEKDAMVNMQDLKSNDMYTFSHSVNVCVLSLITGISMKYDEEKLRELGMGAM
                     LHDIGKTRIPHEILNKPESLTKEEFELVKKHTSYGYELLKRSDVLSTYASYIALTHHE
                     RHDGEGYPLGLRGEEIHEFARIVSVADVFDAITSDRVYKKRINVNEAVEYLIGMGDHQ
                     FDYNIVRSFIEHITIYPPGTCVLLNSGEKAIVVDVNKKYPTRPIVRILADGEGQIPTV
                     PVEIDLTKNNALLISEIIDDI"
     gene            complement(17197..18153)
                     /gene="whiA"
                     /locus_tag="PKI50_06140"
     CDS             complement(17197..18153)
                     /gene="whiA"
                     /locus_tag="PKI50_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HNT03326.1"
                     /translation="MTFSSVTKNEISKIAVTNKCCQLALLSALIKMTGTLQIHGVERI
                     GITLSTENASIARMLFSLLKNCFDINTRVIVRKNRHLKKNNNYTLYIDSDMGSHEILK
                     MTGILQESDKGMKLNHKLPHYLIKKACCRKAYLRGIFLGSGSISDPEKTYHLELVTNN
                     ESFAEDIKDLMNHYQLGAKVVMRKGNFVVYIKEGENIVNFLNIIGAHNALLKLENIRI
                     YKEMRNNVNRIVNCETANLDKTLNAALRQIENIEYIKNTIGLNKLPEGLAEVAELRLE
                     FRDATLKELSEMMSPAIGKSGVNHRLRKLEQIAENLRDRHRE"
     gene            complement(18170..18694)
                     /gene="lepB"
                     /locus_tag="PKI50_06145"
     CDS             complement(18170..18694)
                     /gene="lepB"
                     /locus_tag="PKI50_06145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNT03327.1"
                     /translation="MDENIKKEVISWVKLILSAFVIAFILKTYVFQIALVNQVSMEPT
                     LYEGEILVIAKVNYLINDPARGDIVVLKDELEDKYLIKRAIGLPNEVIDIRNDRVYID
                     GKELNPDYTDALTRDNGFAKSRVPEEKYFVMGDNRMQSRDSRSDTVGFVSRDNIVGKA
                     VFRIWPFNKFGIVR"
     gene            complement(18715..19731)
                     /locus_tag="PKI50_06150"
     CDS             complement(18715..19731)
                     /locus_tag="PKI50_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNT03328.1"
                     /translation="MKNNSVKNTLHGKGIIPGGPKIVAIGGGTGLSVLLRGLKKHTSN
                     ITAIVTVSDDGGGSGVLREDMGILPPGDIRNCIVALANIEPIMEQLMQYRFREGKLKG
                     QSFGNLFIAAMNDICGSFDAAVREVSSVLAVTGKVLPVTLEDVVLYAELEDGTIIKGE
                     SQIPIKQRALNKRIRKVFLEPGNCMPMPEALREIQEADAVVFGPGSLYTSIIPNILVK
                     SIAEAINSTKAMKIYVCNIMTQQGETIGYSLSEHIKAINYHGNRAPIDYAIANTGEIP
                     QALLEKYKTEDASPVLIDYDIIADMGIKVIEGDFVNIENGYLRHDYNKLAEQIFKLLN
                     EEQK"
     gene            complement(19758..20624)
                     /gene="rapZ"
                     /locus_tag="PKI50_06155"
     CDS             complement(19758..20624)
                     /gene="rapZ"
                     /locus_tag="PKI50_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNT03329.1"
                     /translation="MKFLIVTGLSGAGKSQSIKFLEDLGYFCVDNLPPALLGRFVEMC
                     SESKGHIDKAAIVIDIRVGKFLDQLPVGLDELKELGHTYDILFLEASDETLIKRFKSS
                     RRMHPLSVGGRIIKGIKAERRKLQEIKSKATNIIDTTNMSPSELKQEITHLFVEGGKS
                     EGLIIHVISFGYKYGIPLDSDMVFDVRFLPNPYYVEDLKKFSGADAGVRNYVMQWPAA
                     AGFMDKLNDMLEFLIPHFIKEGKSQLVISIGCTGGRHRSVTMANMLSNKLKEANHRVI
                     VEHRDIEEERYR"
     gene            complement(20641..21372)
                     /locus_tag="PKI50_06160"
     CDS             complement(20641..21372)
                     /locus_tag="PKI50_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT03330.1"
                     /translation="MKLFADYHTHTVYSHGTGSILDNVTAAREKGLSEIAVTDHGIRH
                     FAYGVRIKDIAKMRDEIDRINYNTEGIKVLMGMECNIISTAGDIDMDDSIRKYLDILL
                     VGFHMMVLPKTAGDAVNIYGRNYLSKIFHLGTEGIKEANTESMIRAIRKHKIDIITHP
                     GTRIPLDISLVAREAAKAGTALEINSHSSYMKAKHVIAAAEEGAEFVINSDAHAPEDV
                     GNLQNGLNIAMEAGLTCDQIINAVK"
     assembly_gap    21491..21590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22083..22949)
                     /gene="murB"
                     /locus_tag="PKI50_06165"
     CDS             complement(22083..22949)
                     /gene="murB"
                     /locus_tag="PKI50_06165"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNT03331.1"
                     /translation="MQILYNEPMKNHTSFKVGGPADIFIEPDDMAELVKAIKDLKEQC
                     VSYYIMGNGSNLLVSDKGLRGAVVKIGEKFGSVSINNDIITAESGVLLSTLSKMAAKE
                     GLTGMEFASGIPGSLGGAVTMNAGAYGGEMKDIVEWVEVLDQDLNLKRLKNDEMKFMY
                     RKSIIEPGKHIVLRCCIRLKKGNKDEIDNKMAELAEKRKSKQPLHLPSAGSTFKRPEG
                     YFAGKLIEDAGLRGFSIGGAQISTLHCGFVVNNGDATAKDVYDLIRHVQKTVFEKFNV
                     MLEPEVKILGEF"
     gene            complement(22974..23681)
                     /locus_tag="PKI50_06170"
     CDS             complement(22974..23681)
                     /locus_tag="PKI50_06170"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNT03332.1"
                     /translation="MTEVKSTNKEKQPVIEYNGKKYLRIPVKTHVITKDDSIVDVVSM
                     YAREIVKENDIVFISEKAVAITQGRAYPLEEIKPRPLARLLSKYVTKTPSGIGLGIPE
                     TMEMALRECGVLRILFAAVVSFFGKLFGRKGDFYRIAGYKASSIDGPTPNTLPPYNRY
                     VVLGPENPDQVAKSISDSIKAEVAIVDINDLGGSILGTSKSSLDRKALAGILRDNPLG
                     QCCEQTPMGIIRSAEEQ"
     gene            complement(23760..25109)
                     /locus_tag="PKI50_06175"
     CDS             complement(23760..25109)
                     /locus_tag="PKI50_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNT03333.1"
                     /translation="MTLRKAFTLFLCGILTLYIFSGPVLLEHVKEDYKKSLEPVHEKT
                     EWTGVITLWDYPRLDIRSGTRFSWIKSKIGQFEKQNPGVYIELRELDWNEGPGFLKAA
                     AKIGANPDIAPIGSDYFFISGGYLEELDGYISLEERADFIDSTLDTAMYNGKTYGIPW
                     MMTGYTMLLNTDIFSERNVSLPENGEWTYEQFVEALKQLTYNGIYGFNSFIGPGYYNT
                     FGIIMCDGAQIIDDTTGRYIFNTPEAVSGLTKLCDLKHKYKVSHPDFGAMNENQAWSA
                     FLNGKTAVYIGGSWTVPLLRNIQGNNGINFAVAKFPTGKSDVPLSLSSTVCSYAVFKQ
                     KDAAKKKVCIDFIKFITSKESQKELGYLGYFPVRKSGKYLYENDKEMYTIQQNLYFAE
                     HLPKADNWSEIDLILQTRIKEAVQGNISPEQAVKEAEKLIEQRRKQGVAAEAGSAAP"
     gene            complement(25378..26103)
                     /locus_tag="PKI50_06180"
     CDS             complement(25378..26103)
                     /locus_tag="PKI50_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HNT03334.1"
                     /translation="MKLLLDTHCHTISSGHAYSTIKEIAEEAADMGLKLIAMTDHGPD
                     MQGGPDLFHFGNMRVIPEYIKGVRVLKGVEANIMDFEGRLDVPEKYLKKLDIVIASLH
                     EACIDSGTVEENTRALIGAMSNPFVDIIAHPGNPYYPVDIDRLLQCAKDTGTLIEINN
                     SSFLGSRKGSSENCRIIAAKAKEMGVVLAAGSDCHICYDVGKFNKIEEIFEELEMPEA
                     LVINTDPLKLIDYLNKKGKKVSL"
     gene            complement(26120..26353)
                     /locus_tag="PKI50_06185"
     CDS             complement(26120..26353)
                     /locus_tag="PKI50_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNT03335.1"
                     /translation="MYKKLYKSRTVRTISGVCGGLEDYFNIDVTIIRLIWVALTIATG
                     FFPGIFAYVAAALIIPDEPTGYNPEYKVEKDEQ"
     gene            complement(26480..28342)
                     /gene="uvrC"
                     /locus_tag="PKI50_06190"
     CDS             complement(26480..28342)
                     /gene="uvrC"
                     /locus_tag="PKI50_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNT03336.1"
                     /translation="MISLEDKLKNLPEKPGVYIMRDEYNEIIYIGKAINLKNRVRQYF
                     QNSKNQNPKVAAMVERITDLEYIITDSELEALILECNLIKKHRPKYNVMLKDDKQYPY
                     IKITVKEDYPRLIIVREIKKDGAKYFGPYTDVTAVNRTIDLLKGLFPIRYCNKNISRA
                     IGKERPCLNYHIKKCIGPCRGNVSKEQYRELIDSIIMMLEGKQEEVLKGLEDKMHQAA
                     DNLDFEKAAEIRDNMNSLIKIAEKQKVISSALIDQDIIAFALNESESCIQVFFVRGGK
                     LIGREHFLIEDTKLSDSKEILSSFIKQYYGSDALIPREIILQEEIDEINIIERWLSDK
                     KGGRVKLVVPQRGEKQKLIEMVGRNAEDTLRLLIEKQKNDTEKTMGACRELKDILELN
                     AVPERIEAFDISNIQGVLNVGSMIVFEQGKPNNRNYRRFKIKSVEGANDYESMREIIE
                     RRFAHGLKEREELEAEEKDFELGRFSVFPDVIMLDGGVGQVNAVLPVLRELNINIPVC
                     GMVKDDKHRTRGLIYSGREIELPKGSKVFRLISAIQEEAHRFAISYHKSLRSKTTVKS
                     ALDDIPGIGPARRKALLKSFGSLKNIKKASLEELEEVQGMNRKVAEVVYNFFNS"
     gene            complement(28671..31106)
                     /locus_tag="PKI50_06195"
     CDS             complement(28671..31106)
                     /locus_tag="PKI50_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNT03337.1"
                     /translation="MRFGYFDDLNREYVINTPKTPFPWINYLGQEGFFSLISNTAGGY
                     CFYKDARLRRLTRYRYNSIPWDNGGRYLYVNDSGKVYSLTWMPVKNDAESFECRHGLG
                     YTKIKCLQNKLESEVLYFVPLKDNAELHHLRLVNRGEEDKEITLFSFIEFCLWNAYDD
                     MTNYQRTFSTGAVEVEGSTIFHKTDYRERRNHYSFYSVNRKIDGFDTDRDSFLGRYNG
                     LHEPEAVFEGKSRNSIAEGWNPIASHEIRIKLKPGEQTTLLFVLGYGENGDYEKWESK
                     GRINKSKANELIEKYKDDSYVERAFEELREYWDELLSGYQLLSSEEKLNRMVNIWNPY
                     QCMITFNLSRSASYFETGIGRGIGFRDSNQDILGFVHMIPDKARSRILELAGTMLEDG
                     GAYHQYQPLTGKGNNEIGGEFNDDPLWMILSTITYIKETGDYSILDEKMPYNCDANKL
                     GTLKEHLEKAFEYTMKNLGPHGLPLIGRADWNDCLNLNCFSINPDESFQTCSNRNGKT
                     AESVFIAGLFVYVGREFGRLCRLLGYDAQASGIEEQVDKMRGSIMKYGCDENWFLRAY
                     DYFGNRVGSAENEEGQLFIEPQGICVMADIGLENGMALKALDSVHERLNTGYGLVLQD
                     PAYSEYKLNLGEISSYPPGLKENAGVFCHNNPWIMIAETRMGRGSRAFEYYSKIAPAY
                     IEDISDIHKMEPYVYTQMIAGKDAKNPGEAKNSWLTGTAAWNYEAVTKWILGIRPDFE
                     GLIIDPCIPEEWDGFKVIRKFRNTAYNISVKNPEHVSKGIKRVRVDGKTAASNLIPVF
                     NDELTHDIEVIMG"
     gene            complement(31103..33544)
                     /locus_tag="PKI50_06200"
     CDS             complement(31103..33544)
                     /locus_tag="PKI50_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNT03338.1"
                     /translation="MQYGYFNDINREYVITDPRTPVKWINYIGSLDFGGFIDHTGGAL
                     ICKGDPAVNRIIKYIPQLPASEFKGETLYVRIRGKNAYTLFSPYFVPTLDQYQMYECH
                     VGLGYTRIVSEFYDIRTEALVFVPLGGNREIRDIRITNLRAVPVELDVIPVIEYSHFE
                     ALKQLINADWVPQTMRSECIEEHRGYRTLIQYAYMNKDTRINFFTSNHAASSYETDRR
                     YFLGDNEYGTWESPGSLNNSELGNHEVLRGDNIAAMMHHIGILEPGESRRIITQLGQC
                     RSITEEKESMESFRSEERVNEEFEKLKYFWEEYLSKLQVETPDKAMNTMLNIHNPRQC
                     YITKNWSRYLSLYQLGYGSRGIGFRDSAQDVMGIMDSAPEEGRELIEKLLRVQKRNGS
                     AMHQFNPVTMIANEGDSRDFEDRSKYYSDDHLWIVLAVCAYLKETGNMDFLNKTVPFY
                     EKDKSGMPIEKGTVLEHLERAVGFTGNDLGRHGLPLLGFADWNDTVNLPAGAESVFTA
                     CLYGKCLSEMIELMDFLGKKKLKEMYRGRYQRMRERVNSCGWDGEWYISYFDYEGNPI
                     GSKVNSSGQIYANAQSWAVISGFAGEDRALKSLDSVRSILNTRNGIKLSYPGYKGYDP
                     EKGGITTYPPGTKENGGIFLHSNPWVMIAETMVGNGDRAFEYYSQINPACKNGCIDEY
                     QCEPYCYPQNMLGDEHPQFGLARNSWLSGTASWVYQAAVKYILGVRPHYKGMTIDPCI
                     PRAWKEFRVRKHFRGSIYDIRVRNPEGVSKGIRAIWVDGTVFFRNVLPCFRDNRLHNI
                     EVLMGRDLFLTEEDR"
     gene            complement(33579..34187)
                     /locus_tag="PKI50_06205"
     CDS             complement(33579..34187)
                     /locus_tag="PKI50_06205"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HNT03339.1"
                     /translation="MKWLFNTEGIIYKIMTAVYQMIILNLLWCITSIPLITVGASTTA
                     LYYVIGKIARKEEVHEIKDFYKSFRENFKQATCIWMILCAAYVMIYTNLSFLEKYGSM
                     GGLMLAVQLPVLVQVVIITVFVFPLLSRYESSTIGIIKASWVLGIKHVFSCFGTLAIV
                     AAAALIARTVPGLFLLVFISFTAFAIYLVVGRVMEKYRPGAV"
     gene            complement(34280..35800)
                     /locus_tag="PKI50_06210"
     CDS             complement(34280..35800)
                     /locus_tag="PKI50_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT03340.1"
                     /translation="MFKTGKLLCLTLVLVFALASLTGCAKPAAAPAPEPAEAGSGTTL
                     EPVELTWFFIGNGQQEDVSLIEEKINEYIKDKINATVKLQCYQWGAEYDDKLRQMIAS
                     RQEFDICFTAAWANNYMQNAVKGAFVPLNDEKDLLSKYAPKTKALLGEDFLAGSQIDG
                     INYGIPANKEKAHSWGFIIKKDLVDKYGFDLSAIKTLEDLEPMLKVIKEKEPNVYGLE
                     ALNGESPYKLMDWDVIGDDKCPGVVYNDSKDLVVFNEFEKPETMALLKTIRKFFQAGY
                     IRKDAATVTDYNADEAAGKIFAATKSLKPGKDAEMSLTHGFEWVQVYITPPVISSRDT
                     MGSLQAISSTSKNPERALMFLELFNTDPYLNNLINFGIEGKHYVKVSDSVIKAGPDQT
                     KYNPGTGWMFGNQFINYLYESEDPKKWEQFEEFNQQAAGTQTLGFNFDPSPVKNEIAA
                     VNNLWDKYVPALETGSIDPEVNVPKFLEELKAAGIDKVIAEKQEQLNEWAEKMGKK"
     gene            complement(35861..>36042)
                     /locus_tag="PKI50_06215"
     CDS             complement(35861..>36042)
                     /locus_tag="PKI50_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT03341.1"
                     /translation="IYLSNNAGSINNATQIMANLPSQTARMAMAVVAIGPIILAYPFF
                     QKYFVEGLTIGSIKG"
BASE COUNT         8582 a         9281 c         6425 g        11654 t
ORIGIN      
        1 tgtttgcata aaagcctatc ttcacatccc tgtccattcc tacgggaaac tgtgctttat
       61 tcctatatcg ccaagagtcc ttcattccta ttgcatcatt tacggaaacg cctttaaacc
      121 ctccgatccg ttccagagca tccttcacct ggagggtttt gtaccgaagc tgtgcttcat
      181 agctcatatg ctgcagctga cagcccccgc acctttttac gactccgcag gaaggttcaa
      241 tcctgcaggc tgcagccttc agtgtcttca gcagcttgcc gaaggcatag ctcttcaata
      301 cttttactat tcttatctcc gactgttctt taggaagtgc gccttccaca aacactgtaa
      361 acccttctat ttttgccact ccaaggcctt cactggtaag tccggttata tcaactatat
      421 aattccggtt cttttccacc ggtatcgttt tttccatcga cttttctccc caattgaata
      481 tttgagctgg tcttttccat ttccatctta atattttcaa gctccttttt caatagtctc
      541 tgctctctaa ttatttggtc cgtcttcatg tgcagatcct caagtattat ttcggatttc
      601 agatttataa ggtactcatt ttccgagtgt aatctgtctt tctgggcttc cctgttctgg
      661 ctcatcatta taataggcgc ctgtatcgaa gcaatgcagc cgagtacaag attcagaaac
      721 acatatgggt aacgatcgaa aggcttatcc agaacaatct tggaattaat aaacgcccat
      781 gccagtacta caatagcaaa gaatccgaga aatgaccagc ttcctgcaaa cctggcgatt
      841 ttgtcagcca tcctttcacc aaaagtcagt gtcttgctca gttccttatt aatgtccaat
      901 gacctcttcc ctctgataag ctcgtgaatt tgttcctccc gttcttcttc atcggagatt
      961 ataatgtctt tcagatcatt cttatccata gtcacactct ccaatacttt tagcaatatt
     1021 tttgcttata tgaaaataaa tatacagtga taaaattcac tgcacatttc tcacatcctt
     1081 cagattagtt ttcagctctc tcaggcagag ctgaatattt ccaaggcata ctttcagttc
     1141 ttttatgtat tccttatttt catatgtgtt gcccttaata agctttatta ttgtattctc
     1201 cagccttttt agtattttcc tgtcctcact tccaattaca agcttaattg aaaggcgaga
     1261 tgccactttg taaatcgaaa tgcaatgttt gtaaccgaca agtgaatatt tgtaaacgaa
     1321 acgcagcttt tgtaaccgag atgctacctt tgtaaatgaa aagccatttt tgtaaccaaa
     1381 atgcagtttg attttatggt ttttctacgg tatgataata gaaaattgca tgccaaacgg
     1441 aggtcaattc cctctcgatt tttttgtaat cggagaatcg acccctgctt tatgggttag
     1501 catggatagt tggcggcagt tttttagttc tatcttttga gaagagttgg ttttaacagg
     1561 atgtcatcgg ctgctaccgg agaacatcca agcttgtgta gaacctcttc tccgtggtag
     1621 aagctgaacg tttcgtatgg gctacgattg ttcagctttt ttcttttgta ggaattgatg
     1681 tggttcatca tccgagtgat atcctcctgc tccaagttat tgaagcatgt ccccttgggg
     1741 agcaccctgc gaatcaggct gtgattgact tcaatggcgc ctttctggtg aggactgcct
     1801 gcatcacagt aataaatata cgttcgcaaa gtgccgttct gataaggtcc gtattcgatg
     1861 gccttgggat tagaaaactc actgccgttg tctgtcagaa taacagggaa tagctttcta
     1921 aacagttcac gctctaactt ttgatcgagg tcattgaata tatcaatcac cgattgcgag
     1981 gtattggatt cacgaagata tgcaagcatc agattcgttt ctacaaagtg tatcgtcagc
     2041 atgcatttgc cacccttgct tccaatgaca gagtccattt gaacaatcgt tgtatccgga
     2101 ttcttgtcta tgaagctctg gaatgcctca tagttgcgtc caatcctaca gcccttgtcc
     2161 actttgaatt ccggtttttt gtagcgctca cggaacttca cttttctggg caaatcgatg
     2221 ttgcgcacat ccagaagaca ggcatcgatg tagttgtaga tggttttctc gctgcacatg
     2281 agctcatctt catgggacac ataaatctgg tggatggatt gcccctgcag cactagtgga
     2341 ctgataattg cgttaagtct ggcaatctct ttttcgcaga tacacaggcc gctgcgggat
     2401 tccgatacgg tttcatgagc tttgatgtgg gcatgctccg catcgtagag atttttcatc
     2461 agggtacatt tgttgatttg ttcgcaacca ttacagatat aaggcacccg gaatcgagcc
     2521 gtacagattt cttcaataaa gtccggacag ttgtcgttgc accttgggca aagcttgcag
     2581 tatactgacg aagtccttgt gcattccttg ccgcagatac ctttctttct gcagttgaag
     2641 cggttcttgc aagcattgta tgaaaacccc ggatacccgg tggcaacttc cgaaagatat
     2701 ttccggactt ctctggaaat cgtggtcggg ctcttatcca atctgcgggc gatttcttta
     2761 aaggatagac tttctttaag atatttctga agggtcagcc tttcttcgta agtaaaatat
     2821 ttcgacatgg taactctcct tttcgccgcc aacctattca gaatacctcg catcgaaaag
     2881 gtgtgcaaac aaaaagactg gttcatcacc aatcttttgt aagccaggat gcaaccacct
     2941 ggtgtcaata aattctatag cttaataaag ttcaattcga atcgataggg ttgcatttcg
     3001 atttgcaatt gacgcttcca attacaatca tattgcttat accttgtatt atatagcctt
     3061 tatatagatg tatatcttct acctttaaaa gaagctccaa aaaatcatcc agtaccgatt
     3121 ccttttctcc catcagaagc ttaatctccg tatatgtgtt aaagcatatg aaccgcattg
     3181 ccatatcatc atcttcaata catttaaatg ccttatcaat ataactgagt gcctttttat
     3241 actgccccag tattttataa actatagcaa tattgttata tactttgctc tttactctca
     3301 tatccttttg atctgccagg caaagagcct tgctgtaata tctgagtgct gccttaaaat
     3361 ccttctgcct cttaaacaac aggccgatat tcataataat aagtgcggta tccttatcat
     3421 cccccgagta taacaatgca tttttcagct tctgctcgga ataataataa tcctgcatat
     3481 tgctcagaag tatcccgtat ctgtaataat ggagatatga tatttccctg cccatttccg
     3541 aaacctgcaa aagcagttct tcggccttct cataatattc tacggagcgt atatattcgt
     3601 gattataaaa atacatgtat cctaattcag agtaatattc aacaagaaga ttcaaacaat
     3661 cttcgcgtct gactgtatct atagcaagtt tcattgattt tacggcatta ctgcaattat
     3721 tcatataaaa gtgatacatt ccaatgttcc tgtgcatttt tgcagcttct atcatcattc
     3781 cttcattttt gcacagatcc cttaactttt ccaatgcatt gatgtcttct tccaaataat
     3841 attctcttat tctcttcgtg attaattcaa cattatatgc tatttgctga tgacctgata
     3901 ttcctgcaat atcatctgct tttaatttca acagcctcac ctccattaac caatttatca
     3961 aaaatattac agtgttttac ttttacttta cagtgtttta cttttacttt acagtgtttt
     4021 acttttactt tacagtgttt tacgctgaat tgtcttatat tttatataaa ctgcgctttt
     4081 tcgacatccc tctgcaactc atatacgcaa ataaaaaaag tggccttttg accacatttt
     4141 tttcgatttt tactgcccaa aggtttgttt aacggcttca agcatttctc tgattggaag
     4201 ctgatacggg cactttgcct cacataaccc gcatttctca cagtcgctgg cccttgaagg
     4261 catattatta tatcttgttt gagcccactc cataagatta tacctcttgt aatagctttc
     4321 cataactaat actacaggta tattgatccc cttggggcag ggctggcaat aaccgcatct
     4381 cctgcaaaag cgttctccca attcggaggc ctcctttgaa agggcttcct tttcatcctg
     4441 tgacaacggc atttcctcta ttgcctttat attatccatc aattgttcta tgctgaacat
     4501 tccgggtatt gcacaggtta tatgctgatt ctccagtata aacttcaatg atgccctggc
     4561 atttgtaatg gcacctcccg caaatggctt cattgcaata gttccaagat ttttttcata
     4621 tgcttttctg aacaactccg ttccctgagt ttcaactgca ttatatggga attgaatcgt
     4681 ttcaaaaaca tcatattcaa tggccttttc aataacctcc aagctatggc ctgttattcc
     4741 gatgcttttt ataattcctt tatccttgta ttcaagcatg gcctcaatcg ctccgccttt
     4801 tgccattgct ttttccaact cctgcaggct gactatatta tggaattggt aaagatcaat
     4861 ataatctgtt ttgaaattat ttaaagagat ttctatatcc ttcttaaatc cttccctatc
     4921 ccttgccatg gattttgtag ccaggaacac ctgctgcctt ttccctgaca gtgcgcctcc
     4981 tatcaggctt tcacttacgg tgtatcccct tgcggtatca atgaaattta taccctgttc
     5041 caatgccttt tctataaact catttgcatc atcctgattc agctgttgaa ggggtatgcc
     5101 tccaaggctt attcccgata ccttcattcc tgttttccca agcattctgt actgcatgta
     5161 ttattcctcc ttgtcctttg atagctcata caatgagctg taaagattgc tgcaatgctt
     5221 ttttatattt attggccttc cgcttctgtt tgcaaaggca tggactgttt ctccttccgc
     5281 cagcaatgct ccgtctgaag tcctcaaaag cttgtaggag aaagctatcc ttgcaacggt
     5341 catttccttt ataccggttc tgaccaggat ttcatcatca tacactgcgg gaattctata
     5401 tttgcaatgg gtttctgtca acggaagcat tatccccagt tcttccattt ctttgtatga
     5461 tatccctcct gcctcccgaa ggtactcagt ccttcccatt tcaaaatata tgtaataatt
     5521 cggatggtat attattccca tctggtcagt ctctccgtac ctggccctta gcctcgtctc
     5581 atgcactttc aaattatcat ctccagacat gtattcatac agcagaatag cccaaaggct
     5641 gttccctgct ctttaaccca ttagtaatac ccatgaattc caagccccgc acctcgaacc
     5701 tcgcagccta gcaaccggca ttacagcact cttgccgctc ccctgtatat gagtccccgt
     5761 gccgtatcca tagtgataag ctgtccgttc cttaaaatgc ttactgcatt ttccactccg
     5821 actattaccg gtttcccaag gttgattccc gcaatggctg catgggatgt tatcccgcct
     5881 tcttccacaa taaatgccga tgctttttcc ataaggggta tcatctctat atcagtagat
     5941 cttgcaacca atatatctcc ttcccggagc ttgccgaaat ctccgggtat atcatcaaca
     6001 attgaaacct ttccggtaac agacctgtca cctattccta ttccttttga cagtatctct
     6061 cctactgtat gaaccttaat aaggtttgtg ctgcctgccg ctccgatacc tgccgttata
     6121 accacaagat cgccgttctt tataagcttg gattcaacag cagcatctac agcccggtca
     6181 attatatcat ccgtcctctc ggtaaaaggc acatatatgg gataaacccc gaaactcagg
     6241 caaagctttc tcattacacc ggcatcagtg gtagtggcaa ttatcggcgc ctttggcctg
     6301 aatttcgata ccatccttgc tgtagaaccc gattgtgtag gtgttataat ggcagctgcc
     6361 tgaagctcaa atgcagtagt gcatgagctg tggctaatgg catttgctat tgttattctt
     6421 cttcctgaaa agctgcttag gcttttcccg taatccagtt ctctttctat tctctcggca
     6481 attgatgaca tggtcagtac ggtttctacg gggtatttcc ccgctgctgt ctctcccgac
     6541 agcattattg catccgtacc gtctattatt gaatttgcca catctgtcac ttctgccctc
     6601 gtaggcctgg gattccgcat catagagtcc agcatctgtg ttgccgttat aaccggcttg
     6661 cccgcttcat tggatctctt aatcagaagc ttttgagtca gaggtatttc ctcagtggga
     6721 atttcaactc caaggtcccc tcttgcaacc ataaggccat cgcatactct gaggatatcc
     6781 tttacattat caacaccctc ctggttttct atcttcgcaa ttatctgaat atgccctccg
     6841 ccgttttcct ccagaatctt tcttatctcc agcacatcgg cggcttttct tataaatgaa
     6901 gctgcgataa aatccacttc attttctatc ccgaaaacaa ggtcggcgac gtccttatca
     6961 gtaatagcag gcagcttgga agtagctccc ggaatattca cattcttcct gtctccgaga
     7021 gtacctccgt ttaccacccg gcatatgaca tcagtctcat tcttatccag aaccctaagc
     7081 tctatgaccc catccgctat aaggattctg gagccgacag caacttcatc gggcagttct
     7141 ttgtatgtaa tgctgcactg tgtgccgtct ccctgtatat ctcttattgt cagtgtgaag
     7201 tcctggcctt tttccagttc ggcctttccg tccttgaagt agcccagcct tacttcaggc
     7261 cccttggtat ccagcataat tgccacgggt atgccaagct tctctcttat cctctttatg
     7321 acatcaatcc ttctcttgtg ctcggcatgg tccccgtggg agaaattaag cctggctaca
     7381 ttcattccgg ctgttataag ccggcttatc atctcttcgc tttcggttgc gggtcctatg
     7441 gtacacacta tctttgtttt tctcatatta tttcttttcc catttctcaa gctttttttc
     7501 tataagcttt ttattcagcc ttgcgtatct cggaattccg ttttccatgc ggatcttgac
     7561 ttcaccgatt acgagaggcc ttatataatc caggagcggc tgctctatgt aattgccctc
     7621 cttatttatc cattccggcg gaatcttctt ttccttattt gcaacatcat caaggtcaat
     7681 aagctgtgtt tcgcaggtgt attcctttcc tttgcccctt accataccga ccatctttcc
     7741 tgtatgacct tctatcgcat actcaactgc tgcttttccg cacatatagc tttcttcatt
     7801 atccgtaagg gacgcccaat gagcggcgct tctctgtatg gtactgtaat ttaccgttct
     7861 gaccttcttg catacgtttt ccaggacata acgttcaagt acctcgctgg cgcctcccaa
     7921 ctgcacatgt ccgaaagcat cccttcttgc attggggtct tctatttcgg aaatatactt
     7981 gccgtcttcg gtttttattc cttcagaaaa agctattact gcatagttgt atttatccag
     8041 ggctgatttt acatccttgc cgaatttttc gaaggaaaaa ggcacttccg gcaggtatat
     8101 gaaatgcgga gcatcatctt caacttcctt tgccaaagcg gcggaagcag taagccagcc
     8161 tgcgttccgg cccatcaatt cgcatattgt cacggtagga tagtcgtata cccttgcatc
     8221 aaggccaacc tcttttattg aagttgcaat gaactttgcg gcgctcccaa agccgggggt
     8281 atgatctgta ataggcaaat cgttatctat agtcttgggc acccctattg acttcagctc
     8341 gtagcccaca tttatagcat attggcttac cttgtttgcc gtatccatcg aatcgtttcc
     8401 gcctatgtaa aagaagtacc ttatattgtg tgcctgaaat acatctataa tccttctgta
     8461 atccccttca ctggcttcaa tggacttcag cttgtacctg caggagccca gggcggatgc
     8521 gggtgttacc ttcatcagct caatttcctc gggtgattcc tcattcagca ggaacaggtc
     8581 ttccttaagc aaccccgcca caccgtgaag cgccccgtaa accttatcaa tttcaccgga
     8641 cctgaatgcc tcctgtataa caccgcaagc gctggcatta attacagaag tcgggcctcc
     8701 ggattgtgcc accaggcaat ttccctttaa tgcagccata attcatctct ccttttcaag
     8761 ttttgttatg ttatataata agtctcgtgc ctattatcta ctgtagtttg attgccaact
     8821 cataaagctc ccgcgggatt acttttttca tactgagggc ttctcctatt tccatatcta
     8881 tcagcttatt atcccttata cccactaccc ttgacgattt tccttccgcc agcagctcca
     8941 ctgccctggc accaagcctg cttgccagta tcctgtcaaa ggcggttgtg cttccgcctc
     9001 tttgtatata ccccaatatg gttgacctta cctcaatatc cacattctcc attatctgct
     9061 gctccagctg ctttgcgtca cctgctcctt ccgcaaggat tattatactg tgaagcttgc
     9121 ctttgttttt cctctctatc agcagcctgc agatatcatc cagctcaaat cccatctcag
     9181 gtactatgat gccttccgct cctcccgcca gtcccgcata cagcgctata tccccggagt
     9241 accttcccat tacctcaata atacttgccc tttcatggga ggatgaagta tctcttattc
     9301 tgttaattgc atccagcaca ttatttacag ctgtatcaaa gcctattgaa taatcagtat
     9361 aagccaggtc attgtcaatg gtgccgggca cccctatgca ggcaacgcct ttttccgaaa
     9421 gccttttggc tccggcaaaa gacccgtccc ctccaataac caccaaaccg tcaatatcaa
     9481 aaatctttgc cattttggct gccttatcct ggccttcttc tgtcttgaat tcttcacttc
     9541 tggccgtctg cagtatagtt ccgcctcttt gtataatatc agatacggag gattcgttca
     9601 ttttttcaaa atccccgtta ataagccccc ggaagccctt ccttacccca aatacattaa
     9661 acccgctgat tattcccatt cttacaactg cccgtatggc ggcgttcatc cccggcgcat
     9721 cgcctccgct tgttaatact gcaatattct tcatgggtgt acctcctttt gtcctagatg
     9781 gtcataatat gtcccaatat tgtatgaaaa tatatttaat atagtgtctg ataccatatt
     9841 aatatccaag ttcatataaa atattgtgtg cttcctccac ctcagattca agtaccaata
     9901 tttcaacata actgtcttct tcaccgatat ttttgtttac cggctgcagt ttaacgagaa
     9961 ccccctcgtt tgtcatcagc ctgcttatct tttcggcgat tttcttgttc tttgccagat
    10021 atacaactgt ccacataatt gttgcctcct ttattgtcaa acctttatgt ccgcaaaata
    10081 taacatatac ttcaaatctc tgttggtgga gcaacttctt gcttagaaat aattttaaat
    10141 gaaatgccct ttaatatcaa gattattcta tatatcgtta cttgactgca acacaatcgg
    10201 atcctgctac ctctttcagc tcattaagca gcttatcatt tatatcaacc caaatactgc
    10261 tgtcagcttt cattaccctg ctctttccgt tatccttatt agctttgtct accaaaatta
    10321 ccggctgtgt ccccttatat tcaatcagca cttttttcaa ctgctcaata atatccggct
    10381 gctgcccggt gtttattctt atgtaaagct tcctgcctgc atactttttc aaaggcttga
    10441 cagcatcaca tattatcttt gcagcctctt cttccttctg gcttattctg ccctccacca
    10501 gaactattgt atcctgaaca agaaggctct tgaatctgtc atatattttg gggaacacta
    10561 ttatttccag agtaccgtac aaatcctcca attccacaaa agccatcata ttgtttgttt
    10621 ttgtggtttt ttgctttaca gatactatga tgccccccat gatcaccctt ttcccgtcca
    10681 actcggaatt cctttcaata tcctgtgctt cctcctcatt ttcgggggtc agctctgctg
    10741 ttgttattgt cactgatgcc tggagttccg acttatactc ttcaagggga tgcccgctta
    10801 tatacagccc cagcatctct ttttccatgg aaagcatgtt tttcttagga tattcattaa
    10861 tatcgggata tatgtttcct attatattct cactgtcatc tatggtatca aacagtgaaa
    10921 tctggccctc cacattgttt ttcttctggc cttgtatgct ttccataagc ttttcgtaca
    10981 ccgccagcat acggctcctg tatactccaa agctgtcaaa ggcgccgcat tttatgaggc
    11041 tttccaccgt tctcttgttt ataatgctgt aatccatctt ctgaagaaag tcatacaacc
    11101 cgtcaaacct tcccttttcc tgtcttgctt tgataatgga cagaactgca tttattccta
    11161 cgttctttac tgctgccaat ccgaacctta tcttgttctc ctttaccgtg aagcttacgc
    11221 tgctttcatt cacatccggc ggaagcacct caatgccctt gcttttgcag tgctgtatgt
    11281 attccgctac cttcttgctg tttcccataa cactggttat aagtgccgct gaaaactcca
    11341 cagggtaata atactttagc catgctgtct gataagctat tatggcatat gcagccgcat
    11401 gggacttatt aaagccgtag cctgcaaact tggctacctc atcgaaaata tccgctgcag
    11461 tatctgcagg tatcccattt ctgacacagc ccttaaccac taaatttccg gcttcgtcaa
    11521 ctatgccgtt tatgaagttt tccctctcct ttgccattac gtcggctttc ttttttccca
    11581 tggcccttct cacaagatcg gacctgccca tggaatagcc tcccaggtct cttactatct
    11641 gcattacctg ttcctgatac actatgcatc cataggttac atccagaata tgttcgaggg
    11701 cgggatgcat atatctaatc ttatccggat tgtgcttgtt tctgatatat ttaggaatct
    11761 gatccattgc tcccggcctg tacagggata tccccgctat aatatcctcc aggcagttgg
    11821 gcttcagctc cttgaagcac tgggtcattc ctccgctttc caattggaat attccgtaag
    11881 tctctccctg ggatatcatt ttgtacacat tggggtcatc ataatccatg ctgtccatat
    11941 cgggggcttc cattcccttc gcctttataa gctccagggt atcccttatc actgtcaggg
    12001 ttctgagtcc caaaaagtcc attttcaaga gccccaactc ctcaagggtg cccatggtga
    12061 attgtgttgt tattacatcc tcgttcattt gcaggggaac gtattcagtt acggccttcc
    12121 ttgatatcac tactcccgcc gcatgggtgg agctgtggcg tggcattccc tccaccgcct
    12181 tggacatatc tataagctgt cttgccgttt catcccccaa ataaagctcc ttaagctccg
    12241 gattgctttt cagtgccttt tctatagtca ttcctatctc aaaggggatt ttttttgcaa
    12301 tcatatccac ctgggcatac ggaatgttca gtgctctgcc cacatccctt atagcggctc
    12361 ttgcagccat ggtgccgaag gtgattattt gagccaccct gtccttaccg tattttgaca
    12421 caacatagtc aataacctcc tgacgccttt catagcagaa gtccacatca aaatccggca
    12481 tgctaatcct ctcagggttt aagaatcttt caaatatcag attgtatttg ataggatcga
    12541 tattggttat tccgaggcag taggcaacaa ggcttccggc tcccgaaccc cttccgggcc
    12601 ctaccattat cccgttgtcc cttgcaaact ttacataatc ccaaacaata agatagtaat
    12661 cggaatatcc cattctggtt attaccgaca gctcatattc cgcccgctct ttgagttcat
    12721 ccgtcaattc ggcgtatctt ttcgccaaac cgtcataaca gagctttctg atataagtat
    12781 ccgaggtata gccttcggga acatcaaaag ccggaagatg cacctgtccg aaattgaatt
    12841 ccaggctgca catttccgct attttcgctg tgttctccag ggcatcggga acgtatttga
    12901 aaagctcgta catttcctcg gaagatttta aataaaactc ctggccctga aatttgagcc
    12961 tgtcttcctc aagaatagtt ttgcctgttt gaatacaaag caaaatatcc tgtgccgcgg
    13021 catcctcctt gtccacatag tgcacatcgt ttgtagcaat caaaggtatc cccgtctccc
    13081 gggacattct tacaacctct tgattaacca gtgactgctc ctttatgccg tgatcctgaa
    13141 gctccagata aaaattcccc tggccaaaaa tctcattgta tgacaatgcc gctctttttg
    13201 cggcctcgta gttaccgtca attatatggc ctggtatcgc tcctgcgaga caggcgctca
    13261 atgcaatcaa ccctttggaa tacttgcgga gtacttccat atctactctc ggcttatagt
    13321 aaaaaccctc cgtgaagccc aaggaaacta actttatcag attattgtag ccttcttcat
    13381 tctttgccaa aagcacaagg tgatactggc tgccatccag cttgggatcc ctgtcgcaca
    13441 ttgttcttgc ggcgacatat acctcacagc cgattaacgg tttgattcca tatttttttg
    13501 cctgcttgta aaactccatc acaccgtaca taacgccgtg atcagttacc gctattgatt
    13561 ccatgcccag ttccttcgct ctggccacaa gcttttcaac tctgcccgct ccgtctaaaa
    13621 ggctgtattc cgtatgcaca tggagatgca caaagttctt cataagtatt tctcccctgt
    13681 ggtacgagta ccacctatct taattttttg tccagctttt cctttaattc ttcataaccc
    13741 ggtttgccca gcagcgcaaa catattcttt ttatattcct caaccccggg ctgatcaaag
    13801 ggattgacgc caagcagata cccgcttata ccgcatgcct tttcaaagaa ataaaccatt
    13861 tccccgaaat aatactcgct gatttcggga atgttgagta catttatcgg tactcctccg
    13921 tccatatggg ctagcattgt accctgaaac gcctttttat tcacaaaatc catcgtcttt
    13981 ccgctgataa aattcaaacc gtccaggttt tcttcatcat ttattatctc tacttcaaat
    14041 ctgggctttt ctatattaag gatggtctca aacatatttc tgctgccgtc ctgaaggaat
    14101 tgccccaggg agtgcagatc ggcagaaaaa ctcattgatg cgggaaatat ccctttcccg
    14161 tcctttcctt cgctttcgcc gaacaactgc ttccaccact cggcaaagaa ttgcagggaa
    14221 ggctcatagt ttatcaggac ttcaatagcc ttgccctttc cggcaagtat gcttctcacc
    14281 gccgcataac ggtaacactg gtttttatcc agttccggct cattgtattc atcccaggca
    14341 tcccttgcac cttcaaggat gcttcttata tcaattcctg ccgctgctat tggaagaagc
    14401 cctacgggag taagcaccga gtacctgccc cctatatcat cggctataac aaaggattca
    14461 tagccttctg catctgcaag ccttttaagt gcgcccctgg aggcatcggt aacggcatat
    14521 atccttttcg ccgcttcctg ctttccgtat ttcgcctcca gatatgactt aacaaacctg
    14581 aaggcaatag cgggctcggt agtagtccct gacttcgata tgacacaggc gcttacgctt
    14641 tttccctcta tcacttccat aagctccttg aagtatgtgc cgcttatatt gtttccgagg
    14701 aaatatatct caggagcgtt tctcttatcc tttggcagca ggttgcggaa ggaatgcccc
    14761 aaaagctcaa cggcagtcct tgctccaagg tatgaacccc ctatgcctat taccagcaat
    14821 acttccgagg aatcctttat ccgtccggca gcttttataa tcctttcaaa ttcatccctg
    14881 tcatagtttt ttggatagtc cacccatccg agaaaatcat tgcccgctcc tgttttttcg
    14941 tggagcatgt aatgggcaga cttcacatat tctcttaaac aagataattc atgatccctt
    15001 atcatggaat tggaacaatc aaattcaagc tttttcataa cttcctcctt cattacacgc
    15061 agttcctgtt aatccccagg aaagccgctc ccagcagccc cgcttcattt ccaagctgcg
    15121 ccaatactat atccccgtat ttgattccgg atgtaaacag catcccgccg acttcctttt
    15181 tcagcttatc cgtaagatag gctccggctt tggatacccc accgcctatt gctactatat
    15241 ccgggtccag aatattgaat atatttatta ttcctattga aagatacttt atcatcctgc
    15301 ctacagtctc aataccaagc tgatcccctt ccttcgccgc atcaaaaatc aacctggcat
    15361 cgatgttttc tgtatccccc gccttatcca aaatgcttga cttaatttta tccctttcaa
    15421 gcctgttgac tgcatacctt atcattgcag ttgccgaggc aaaagtctcc aggcagccca
    15481 tattgccgca gttgcatctg taaaaattgg accctacaat catatgcccg agttcggaac
    15541 ccgcaaagtg gcttccgctg tatatcttat ggcctattat tattccgctg cctacacccg
    15601 ttcctattgt tatcagcacg gaattttcaa caccctttgt ggagccgaaa aggctttcgg
    15661 ccaaagcggc ggcattggca tcgtttccca tatatacagg cttgttgaaa tgctccttaa
    15721 gcataatgcc caaaggaaca ttctcccaga aaagattagt acagtatatg acatatccgc
    15781 ttttctcatt catcagcccg ggggcaccga tgcctatgga ttccatatcg cctatgcctg
    15841 ccccatagtc cttgagcagc ttttccacct gctcctgcat atctttaatt atcagcctgt
    15901 aatctctgcc tgcattagta ggaatggaag actgcagaag tatccttccc tccctgtcca
    15961 ccaaacctgc ttttatctca gtacctccca ggtcaattcc gacaagcatt gccgcacctc
    16021 cttgtacaga cgtcatatat ggttactgtt acggacgcca tatatggcgt ccgtacccgg
    16081 attgttatat atcatctatg atttcactta ttaaaagtgc attattcttt gtcagatcaa
    16141 tctcaacagg cactgtgggt atctgcccct ctccatctgc aagtattctt acaatcggcc
    16201 tggtgggata tttcttgttc acatccacta ctatggcctt ttccccgcta tttaaaagta
    16261 cgcaggttcc aggcggatat atggttatgt gctcgataaa actccttacg atattgtagt
    16321 cgaattggtg atctcccatt ccaatcaaat attcaacagc ttcatttaca ttgatcctct
    16381 tcttgtatac cctgtccgaa gttatggcat caaacacatc tgcaacggat actattctgg
    16441 caaactcgtg tatttcctcc cccctcagcc ccagaggata gccctcacca tcatgcctct
    16501 catggtgggt caatgctata tagcttgcat aggtgctcag cacatcgctt cttttaagca
    16561 gttcatagcc atagcttgta tgctttttga ccagttcaaa ctcctctttc gtaaggcttt
    16621 cgggtttatt caatatctca tggggtatcc ttgtcttgcc gatgtcatgg agcattgcgc
    16681 ccattccaag ctctctgagc ttttcttcat catatttcat tgatatgcct gtaatcagag
    16741 acaatacgca tacgttaacg ctgtggctga aggtatacat gtcattgctc ttcaaatcct
    16801 gcatatttac catagcatcc ttttcatcca taagctcgtc aatcattttg catacgctat
    16861 tcctgatggg tttgacttca agggagtatc cgtatcttaa gctgtccatt gcgttcttta
    16921 tgtttattct ggcttcgaat ctcgtttctt ccgatataac atccttaagt actatatcac
    16981 ggcttatttc atcatcaata tatacttccc agatacccaa atccctgaac tttgcaatat
    17041 aagaattctt cagtatgata ccggcattca gcaatatatt tccgtcattg ctgtacagag
    17101 tcctggcaag aaccattcct tcttttacct tatcaaccgg taattttctc atacatgcat
    17161 catctcctta cggcgcagca agaataatgc tctgtattat tccctgtgcc tgtccctgag
    17221 attttccgct atttgctcca gctttctcaa tctatggttt actcctgact ttcctattgc
    17281 agggctcatc atttcactca attccttaag ggttgcatcc ctgaattcca gcctcaattc
    17341 tgcgacttca gccaagccct cgggaagctt gttcaatcct attgtgttct tgatgtattc
    17401 aatattttct atctgtctta atgctgcatt cagggtcttg tccaggtttg cagtctcaca
    17461 gttcactatc ctgtttacat tattccgcat ttccttgtat atcctgatgt tctccagctt
    17521 cagcagagca ttgtgagccc cgattatgtt caaaaagttc acaatgttct ccccttcttt
    17581 tatataaacc acaaagttgc ctttcctcat tacaaccttt gcaccgagct gatagtgatt
    17641 catcaaatct ttaatatctt cggcaaaact ctcattattt gttaccaatt caaggtgata
    17701 tgtcttttcg ggatcgctta tggatccgct cccaaggaaa attcctctga gatatgcttt
    17761 cctgcagcaa gccttcttta tcagataatg cggaagctta tgattcaatt tcataccctt
    17821 atcactttcc tgcaatatcc ctgtcatctt gagtatctcg tgagaaccca tgtcagaatc
    17881 gatatataag gtgtagttgt tgttcttctt aagatgcctg tttttcctca caatcaccct
    17941 ggtgttgata tcaaaacagt tcttcagcag tgagaacagc atccttgcta ttgaggcgtt
    18001 ctcagtggaa agtgtgattc ctatcctttc aactccatgt atttgcaggg ttccggtcat
    18061 tttaataagt gctgacagca gtgcaagctg acagcactta ttagtgactg caatcttaga
    18121 tatctcattt tttgttacag aagaaaaggt catgttaaac agcccctctt tatcttacta
    18181 ttccgaactt attaaaaggc catattctga atactgcttt ccccacaata ttatcacggc
    18241 ttacaaagcc taccgtatcc gatcggctgt ccctgctctg cattctgtta tcccccatta
    18301 caaagtactt ctcctccggc accctggact tggcaaagcc gttatcccgg gtcagtgcat
    18361 ctgtataatc aggattcagt tcctttccgt ctatatatac tctgtcattt cttatatcaa
    18421 tgacttcatt tggaagccct atcgcccttt taataagata tttatcctcc aactcatcct
    18481 taagtaccac aatatcgcct ctggcagggt cgttaatcag gtagttcacc tttgctatta
    18541 caagtatttc cccctcataa agggtgggtt ccattgatac ctgattaacc agtgctatct
    18601 ggaacacata ggtttttaat ataaaggcaa tgacaaatgc cgataatatc agttttaccc
    18661 acgatattac ctcttttttt atattttcgt ccaatactat ctcccctttg ttaattactt
    18721 ctgttcttca ttcagcagct tgaatatctg ctccgccagc ttattgtaat catgcctcag
    18781 atacccgttc tcaatgttta caaagtctcc ttcaatgact tttatcccca tatcagctat
    18841 tatatcataa tcaatcaaaa ccggagaagc gtcctccgtt ttgtattttt ccaaaagggc
    18901 ttgggggatt tcccctgtat tggcaatggc ataatctatc ggagccctgt tcccatgata
    18961 attgattgcc ttaatgtgct cagaaaggct gtaccctatt gtttccccct gctgtgtcat
    19021 aatattgcat acatatatct tcattgcttt ggtgctgttg attgcttctg caatactctt
    19081 aaccaaaata ttgggtatga tgctggtgta aaggcttccc ggcccaaata caacggcatc
    19141 cgcctcttga atctccctta gggcttccgg cataggcatg caattgcccg gctccagaaa
    19201 gacctttctt atcctcttgt tcaaagcccg ctgtttgatg ggtatttgtg attcgccctt
    19261 gataatggta ccgtcttcaa gctctgcata cagcaccaca tcttccagcg tcaccggcag
    19321 aacctttccc gtcacagcca atacactgct tacttccctg acagcagcgt caaagcttcc
    19381 gcaaatgtca ttcattgctg caataaaaag attcccaaag ctctgccctt ttaatttacc
    19441 ttccctgaac ctgtattgca taagctgctc cattatcggt tcgatattgg caagggccac
    19501 tatgcagttt ctgatatctc cgggaggaag aattcccata tcctccctga gcactcccga
    19561 acctccgccg tcatcggata cagtgactat tgcagtaata ttggatgtat gtttcttcaa
    19621 tcctctgaga agcaccgaaa ggccggtacc tccgcctatc gccacaatct tcgggcctcc
    19681 cgggataatc cccttcccat gtaatgtatt ctttacagaa ttgtttttca aaagtatccc
    19741 cctcgtattg tgttttatta tctatatctt tcctcttcaa tatccctatg ctccactata
    19801 actctatggt ttgcctcctt cagcttattg ctcagcatat tggccatggt aacggaacgg
    19861 tgccgtccgc ccgtacagcc aattgaaatt accagctgag atttcccctc tttaatgaaa
    19921 tgaggtatca gaaactccag catatcgttt agtttatcca tgaatcctgc tgctgcaggc
    19981 cactgcatga cataattcct tactccggca tccgctccgc tgaatttctt aagatcctcc
    20041 acatagtacg ggtttggaag aaaccttaca tcaaatacca tatcggagtc caggggtatg
    20101 ccatacttgt agccaaagga aataacgtgt attatcagcc cttccgattt ccctccctca
    20161 acaaatagat gtgttatttc ctgttttagt tccgaaggac tcatattcgt cgtatctatt
    20221 atattcgtag ctttggattt tatttcctga agctttcttc tttcggcttt tattcctttt
    20281 attatccggc ctcccacaga cagaggatgc atccttctgc ttgatttgaa cctctttatc
    20341 agagtctcgt cggaagcttc cagaaacagt atgtcatagg tatggcctaa ttccttaagc
    20401 tcgtcaaggc ctacgggaag ctgatccagg aacttgccta cccttatgtc tattactatg
    20461 gctgccttgt ctatatggcc cttgctttca gagcacattt caacaaacct gccgagcaag
    20521 gcaggaggca gattgtcaac gcaaaaatac cccaaatcct ccaaaaactt tattgattgg
    20581 ctttttccgg cccccgataa accggtaaca attaaaaatt tcattgacat acctcctgga
    20641 ttattttacg gcatttataa tctgatcaca ggttaagccg gcttccatgg caatattcag
    20701 gccattttgc aggttgccca catcctccgg agcatgggcg tcgctgttaa ttacaaactc
    20761 tgccccttct tccgctgccg caataacatg ctttgctttc atataactgc tgtgggaatt
    20821 tatttcaaga gcggtgcctg ccttggcggc ttctcttgca acaagggaaa tatcaagcgg
    20881 aatgcgggtt ccgggatgtg ttattatatc tattttatgc tttcttattg ccctaatcat
    20941 tgattcggtg tttgcttcct taattccttc cgtacctaga tgaaatatct tcgacaagta
    21001 gtttctgcca tagatattca cagcatctcc ggctgtcttc ggcaatacca tcatatgaaa
    21061 gcccaccaga agtatatcaa ggtatttgcg tatactgtca tccatatcaa tatccccggc
    21121 ggtgcttatg atattgcact ccatacccat gagcaccttt atgccctctg tattgtaatt
    21181 tatcctgtct atttcgtccc tcatttttgc aatatccttg attctgacgc cataggcaaa
    21241 atgccttatt ccgtggtctg taactgcaat ctcagaaagc cccttttccc tcgccgccgt
    21301 tacattgtcc agtatacttc ccgtaccgtg gctgtatacc gtatgggtat gataatctgc
    21361 aaaaagcttt atcaagtact tcacctctga tattaataat atcattatta ttgtcaaatt
    21421 gccacttgtc atactaagga gacacgggga cgtttctcct gtcccacaag gagacacggg
    21481 gacgtttctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcctgtccc
    21601 acaaggagac acggggacgt ttctcctgtc ccacaaggag acacgcgaaa cgtccccgtg
    21661 tcctccgaaa cgtccccgtg tcctccctca cgtaaatccc ccggatttta ctgcgtatct
    21721 tctcttgatt ctgttgccca aagccaataa ctagtcgctg ttatacaagg ccagtgctct
    21781 gcaggatgca tacgaagtaa gagcttatat agtatagcag ggctttaaag tgtaggggtt
    21841 gaacagtttt tggagttaaa aaagcatcta tatataagtc atagtatact tatataaaaa
    21901 ttgtaaatga ctcttattag ctgaaactta gaaacagtcg tatgcgaggc atagacgccg
    21961 cgcaagctgt cacggtcacg gatggccttt gacagcgtta gacttttctt aggttcagtg
    22021 aattagaagt catcaatttt tatatagcta tacgagacta catatagatg ctttttaatc
    22081 tgctaaaact ctcctaatat cttgacctca ggctcaagca tcacattgaa cttctcaaat
    22141 actgtcttct gcacatgcct gatcaggtca tacacatcct ttgccgtggc atccccatta
    22201 ttcaccacaa aaccgcagtg gagggttgat atctgggctc cccctataga aaatcctctc
    22261 aatcccgcat cttctatgag cttgcctgca aaatacccct caggcctctt gaaggtactg
    22321 cctgcgctcg gcaaatgcaa cggctgcttg cttttccttt tttcagccag ttccgccatt
    22381 ttgttgtcta tctcgtcctt gtttcccttt ttaagcctta tacagcatct cagaactatg
    22441 tgctttcccg gttctataat gctcttcctg tacataaatt tcatttcatc atttttcagt
    22501 ctctttaaat taagatcctg atccagcacc tcaacccact caacaatatc cttcatttct
    22561 ccgccgtagg ctccggcatt cattgtcact gctcctccga gagatccggg aatgccgctt
    22621 gcaaactcca ttcccgtaag gccttccttg gcagccattt tggataatgt tgaaagcaga
    22681 acacctgatt ctgctgtgat tatatcatta ttgatactga cgcttccgaa cttctctccg
    22741 attttgacta ccgcccctct cagtcctttg tcggatacca ggaggttgct tccgttgccc
    22801 attatatagt atgatacaca ttgctcttta aggtctttaa ttgcctttac cagttccgcc
    22861 atgtcatccg gttcaataaa tatatccgca ggccctccca ccttgaagga ggtatgattt
    22921 ttcataggct cattatatag tatctgcatt gctgccatgt cccctttcag ttttcactgt
    22981 tcctccgcgc ttcttataat tcccatgggt gtctgctcgc agcattgccc cagtggatta
    23041 tcccttaata tacctgcgag ggccttcctg tcaagggatg attttgaagt tcccagtata
    23101 ctcccgccca aatcattaat atctactatt gcgacctccg cttttatgct gtcgctgatg
    23161 ctttttgcca cctgatccgg gttctcgggc cccagtacaa catatctgtt atacggaggc
    23221 agagtgtttg gagtaggccc gtctatggaa gaagccttat aaccggctat tctgtagaaa
    23281 tctcccttac gtccgaacag ctttccgaaa aaactcacaa cagctgcaaa aagtattctc
    23341 agcaccccgc attcccttaa tgccatttcc atggtctcag gtattcccaa gcctattcct
    23401 gaaggagttt ttgtaacgta tttggacagc agccttgcca ggggcctcgg ctttatttct
    23461 tccagcggat aggctctccc ctgggttatt gccacagctt tttcgcttat aaatactata
    23521 tcattctcct tcactatttc ccgggcatac atactgacaa catcgacaat gctgtcatcc
    23581 tttgttatga catgggtctt tacaggtatt cgcaaatatt ttttcccatt gtattctatt
    23641 accggctgtt tttccttatt ggtactttta acctcagtca tacaatctcc tccatttggt
    23701 ttgtttctta tgtgaaattt gtccatattt ttacagtatt attcaacgga tattttatat
    23761 tatggggcag cgcttcccgc ctctgcagcc acaccctgct ttctccgctg ttctatcagc
    23821 ttttccgctt ccttaacagc ctgctccgga cttatattcc cctgcaccgc ttcctttatt
    23881 cttgtctgca gaataaggtc gatttcactc cagttgtccg ccttgggaag gtgttccgca
    23941 aaatataagt tctgctgtat agtatacatc tctttgtcat tttcgtacag atatttacct
    24001 gattttctga cgggaaagta acctaaatac cccaactcct tttgtgattc tttggaggtg
    24061 atgaatttga tgaaatcaat gcataccttc ttcttggcgg catccttctg cttgaaaacg
    24121 gcataagaac acacagttga gcttaatgac agaggcacat ccgactttcc tgtcggaaac
    24181 ttggccacag caaagttaat tccgttgttt ccctggatat tccgcagcag cggcaccgtc
    24241 catgatcctc ctatgtacac tgccgtcttc ccattcaaga aagcggacca tgcctgattt
    24301 tcattcatgg ctccgaagtc cgggtgggag actttgtatt tatgcttaag atcacacagc
    24361 tttgtcaggc ctgatacagc ttcgggagta ttgaagatat atctgcctgt tgtatcgtca
    24421 attatctgtg ctccgtcaca cattatgatt ccgaaggtat tgtaataacc cggcccgata
    24481 aaggagttga aaccgtatat cccattatat gtaagctgtt tcaatgcttc aacaaactgc
    24541 tcgtaggtcc attccccgtt ctccggcagg ctgacatttc tttcactgaa gatgtcggta
    24601 ttgagaagca ttgtatagcc tgtcatcatc catggtattc cataggtctt cccgttgtac
    24661 atcgctgtat caagtgtgct gtcaatgaag tctgcccttt cctccaagct gatatatccg
    24721 tccaattcct ccagataccc tccggagatg aagaaataat cgctgcctat gggtgcaata
    24781 tccggattcg ctcctatttt ggccgctgct ttcagaaagc ccgggccttc attccaatca
    24841 agctctctta actcaatata tactccgggg ttctgttttt caaactgccc tattttgctt
    24901 ttgatccagc tgaaccgtgt tccgcttctg atatccaggc gcgggtaatc ccacagggtt
    24961 attacccctg tccactctgt tttctcatga accggctcca ggcttttctt gtagtcctcc
    25021 ttcacatgct ctaataatac aggccccgaa aatatataaa gcgtcagaat cccacataaa
    25081 aaaagagtaa atgctttcct aagggtcatt tgtatccctc cataccggct tttactctat
    25141 gtatattcac ttataccgct caggtattcc tcgccgtcaa agtaccgtat tacacggaac
    25201 acaccggttt cccacggagt cgcttcgcga tttcacggta acttatatat attgtggata
    25261 cattagtaaa tttcgatact tactttgctt tcgatgatta ttaatgtatc ataatatttt
    25321 ccccgccgtg tactcagtgg atttaagtgt tttcagcgtt acccgttccc ccgtaactta
    25381 aaggcttact ttcttacctt tcttgttcag atagtcaata agcttcaagg ggtcggtatt
    25441 tatcaccaga gcctcaggca tttccagttc ctcaaatatc tcttcaatct tgttaaactt
    25501 ccctacatca tagcagatat gacagtcact tcctgcggcc agtaccacac ccatctcctt
    25561 tgctttagct gctataatcc tgcaattttc cgagcttccc ttacggctcc ccaaaaagga
    25621 gctgttattt atttctatca atgttcccgt atccttggca cattgaagca gcctgtctat
    25681 atcaacggga tagtacgggt tcccgggatg ggctataata tccacaaagg gatttgacat
    25741 tgccccaatt agtgcccttg tattctcttc cacagttcct gagtcaatgc aggcttcatg
    25801 aagagaagca atgactatat caagtttttt taagtatttt tcgggcacat caagtctgcc
    25861 ttcaaaatcc attatgttgg cctctacacc cttcagcacc cttacaccct tgatatattc
    25921 aggtataact ctcatatttc caaaatggaa taaatcgggt cccccctgca tatccggtcc
    25981 gtggtctgtc attgctatta acttcaagcc catatctgcc gcttcttctg caatttcttt
    26041 aatggtactg taagcatgac cgcttgatat ggtatgacag tgagtatcaa gtaataattt
    26101 caacaaaaca ccctctttgt tattgttcgt ctttttctac cttatattcc ggattgtacc
    26161 ccgtcggttc atccggaata ataagcgcgg cggctacata agcaaatatc ccggggaaaa
    26221 atcctgttgc tattgtaagg gcaacccata taagcctgat tatcgtaaca tcaatattga
    26281 aatagtcctc caagcctccg catacacctg atattgttct tacagttctg gatttatata
    26341 atttcttata catattatct ttacctcctg gttttaattt gtcaaacatg cttcaatgat
    26401 gacaggcaag aattgcccgg gatagacatg ttcgcttttt ggttttcata atatattacg
    26461 aaacattttt gaaaaagttt tatgaattaa aaaaattata tacaacttct gcaactttcc
    26521 tgttcatccc ctgtacttcc tcaagctcct ccaaggatgc ctttttgata ttcttcaagc
    26581 tgccaaaact cttcaacagt gcttttcttc tggcaggccc tattcccggt atgtcgtcca
    26641 gagcagactt gacggttgtc ttgcttctaa ggctcttatg atagcttatg gcaaacctgt
    26701 gtgcctcttc ctggattgcg gatatcagcc gaaaaacctt ggagcctttc ggcagttcga
    26761 tttccctgcc gctatatatc agcccccttg ttctgtgctt atcgtccttt accatcccgc
    26821 aaacaggtat atttatattc aattccctga gaaccggcag caccgcattg acttggccca
    26881 cgcccccatc cagcattatc acatccggga acaccgagaa tctgcccagt tcaaagtctt
    26941 tttcctctgc ttcaagttct tctctctcct taaggccatg ggcaaatctt ctctctataa
    27001 tttccctcat gctttcataa tcgttggcgc cttcaacgga ttttatcttg aatcttctgt
    27061 agtttctgtt atttggcttt ccctgctcaa aaactatcat tgaaccgaca ttcaatactc
    27121 cctgaatatt agaaatatca aaagcctcaa tcctttcagg tacagcattg agctccagaa
    27181 tgtccttaag ttccctgcaa gctcccattg ttttctcggt gtcatttttt tgcttttcta
    27241 tcagaagcct taaggtgtct tccgcattcc tgcctaccat ttcaataagc ttttgctttt
    27301 ctcccctctg agggacaaca agcttgaccc ggcctccctt tttatcgctg agccatctct
    27361 caataatatt tatctcgtca atttcctcct gaagtattat ttctctgggt ataagcgcat
    27421 ctgacccata gtactgttta ataaagctgc ttaatatctc ctttgagtca ctaagcttgg
    27481 tatcctcaat aagaaaatgc tctctgccga taagctttcc tcctcttaca aagaaaacct
    27541 gtatgcaaga ctccgattca tttaacgcaa aggctattat gtcctggtct atcaatgctg
    27601 aagatattac tttctgcttt tccgcaatct ttatcaggga gttcatatta tccctgattt
    27661 cagccgcctt ctcaaaatcc agattatctg ccgcttgatg cattttgtcc tccagcccct
    27721 tcaatacttc ctcctgcttg ccctcaagca tcattattat tgagtcaata agctccctgt
    27781 attgttcctt gctgacattt ccccggcatg ggcctataca tttttttata tgatagttga
    27841 ggcagggcct ttcctttcct attgccctgc ttatgttttt attgcaatat cttatgggaa
    27901 aaaggccttt caggaggtca atggttctgt ttacagcggt aacatcggtg taaggcccga
    27961 aatactttgc tccgtccttc tttatctctc tcacaataat aagcctggga taatcttctt
    28021 ttactgtaat cttaatatag gggtactgct tgtcatcctt aagcatcaca ttatatttag
    28081 gcctgtgttt ttttatcagg ttacattcca gtataagggc ttccagctcc gaatcggtta
    28141 ttatgtattc caggtctgtt atcctctcaa ccattgcggc aacctttgga ttctgattct
    28201 ttgagttctg gaaatactgc cttaccctgt ttttcaggtt tattgccttg ccaatatata
    28261 taatctcatt atattcgtca cgcattatat aaactccggg tttttccggg agatttttca
    28321 gtttgtcttc caatgaaatc atcagtgtat tcctccggtt ttgaatagtg tatgcttcta
    28381 ttatactaca agtttgctga aataaaacga ggcaaaaaga tgtcccccac ttctacaagt
    28441 ggcgtgtacc gctttcgtta cccaacaggt ggtgagcata tcgcccgcct cgttgaaacg
    28501 ctcgggtagg tttttgcaag tgtgcaaaaa ccctcattga aatcttgtta taaaaaggag
    28561 ctgcgggacg tttcgggaca tggggacgtt tctcctgtcc cgctgaaagc gggacaggag
    28621 aaacgtcccc atgtcccgaa acgtccccgt gtcccgaaac ccgtgtcctc ctatcccatt
    28681 attacctcaa tatcatgagt caattcatcg ttaaacaccg gtattaaatt gcttgcggca
    28741 gtctttccgt ctaccctcac cctttttatc cctttgctga catgctccgg gtttttcacg
    28801 cttatattgt atgctgtatt cctaaatttt cttataacct tgaagccgtc ccattcttca
    28861 ggtatgcacg gatcaattat aagcccttca aagtcaggcc ttatacccag aatccatttt
    28921 gtaaccgctt cgtaattcca tgcggctgtt ccggtcagcc aggaattctt tgcctccccg
    28981 gggtttttgg catcctttcc ggcaatcatc tgtgtatata cataaggttc catcttgtga
    29041 atatcgctta tatcttcaat atatgcggga gcaattttcg aatagtattc aaaagccctg
    29101 ctgcccctgc ccatccttgt ttcggcaatc ataatccacg gattgttgtg gcagaacacc
    29161 cctgcgtttt ccttcaatcc cgggggataa gaagatatct cgcccagatt gagcttatat
    29221 tcactgtagg ctggatcctg aagcaccaaa ccatacccgg tgttcagcct ttcgtgaacc
    29281 gagtccaatg ccttcagcgc cataccgttt tccagcccta tatcagccat tacacaaatg
    29341 ccctggggtt caataaacaa ttgtccttct tcattctcgg cacttcccac tctattgccg
    29401 aaataatcat aagccctgag aaaccaattt tcgtcgcagc catacttcat aatactgccg
    29461 cgcattttat ccacctgctc ttctatgccg gatgcttgag catcatatcc cagaagcctg
    29521 cagagccttc cgaattccct gccgacgtat acaaacaacc ccgcaatgaa taccgattcg
    29581 gctgtctttc catttctgtt gctgcaggtc tgaaaggatt catccgggtt tatggaaaaa
    29641 cagttcaaat tcaggcagtc attccagtcg gcccttccta ttaacggcag tccgtggggc
    29701 cccaggtttt tcatcgtata ttcaaaagcc ttctccaaat gttccttcag cgtgcccagc
    29761 ttatttgcgt cgcaattgta gggcattttt tcatcaagga ttgaatagtc ccccgtttct
    29821 ttgatatatg ttattgtgga taatatcatc cataaggggt cgtcattgaa ctctccgcct
    29881 atctcattat ttccttttcc cgtaagaggc tggtactggt gatacgcccc tccgtcctca
    29941 agcattgttc ccgcaagctc caatattctt gaccttgctt tatccggaat catatgcacg
    30001 aatcccagga tatcctgatt ggagtccctg aagcctatcc cccttccgat tccggtttca
    30061 aagtaggaag cgcttctgga taaattgaag gttatcatac attggtaggg attccaaatg
    30121 ttcaccatcc tgttcagctt ctcctcgctg ctgagcagct gatatccgga cagcagctca
    30181 tcccaatact cccttagttc ctcgaaggct ctttccacat aactgtcatc cttatatttt
    30241 tctataagct catttgcctt tgatttgttt atccgccctt ttgactccca tttttcgtaa
    30301 tccccgttct ccccatagcc cagcacgaaa agaagtgttg tctgctcacc gggcttgagt
    30361 tttatcctga tttcatggga agcaatggga ttccatccct ctgcaattga attccgcgat
    30421 ttcccttcga aaactgcctc aggctcatga agtccgttgt atcgcccaag gaaagagtcc
    30481 ctgtcggtat caaaaccgtc aatcttcctg tttacgctat agaaggagta gtggttcctc
    30541 cgctccctgt aatcagtctt atggaatatt gtgcttccct ccacctcaac cgcaccggta
    30601 ctgaaggtac gctggtaatt ggtcatatca tcgtaagcat tccacaaaca gaactcaata
    30661 aaggaaaaca aagtgatttc cttatcctct tcccctctgt tcaccagacg aagatgatgc
    30721 agctcagcgt tgtccttgag gggcacaaaa tacagcactt cactttcaag cttattctga
    30781 aggcatttta tttttgtgta acccaaacca tgcctgcatt cgaagctctc ggcatcattc
    30841 ttgaccggca tccaggtcag tgagtaaacc tttcccgagt cattgacata cagatacctt
    30901 cccccattat cccacggaat gctgttgtac ctgtatctgg taagccttct gagccttgca
    30961 tccttataga agcagtagcc ccccgctgta tttgaaatga gcgaaaaaaa accttcctgt
    31021 cccaaataat ttatccaggg aaagggagtc tttggtgtat ttatgacata ttccctgttc
    31081 aagtcatcaa aatatccgaa tctcatctgt cttcctccgt caaaaaaagg tctcttccca
    31141 ttaaaacttc tatattatgc agcctgttgt ccctgaagca tggtaacaca tttctgaaaa
    31201 atactgttcc atccacccat attgccctta ttcctttgga aacaccctca ggattcctca
    31261 ctcttatatc atatatcgaa cctctgaagt gctttcttac cctaaattcc ttccatgccc
    31321 ttggtatgca aggatcgatt gtcatgccct tatagtgggg ccgaactccc aatatatact
    31381 tcacggctgc ctgataaacc catgaagccg tacctgaaag ccagctgttt cttgcaagtc
    31441 cgaactgcgg atgttcatcg cccagcatat tctggggata gcagtaaggt tcgcattggt
    31501 attcatctat acacccattc ttgcaggcag gatttatctg gctatagtat tcaaatgccc
    31561 tgtcgccgtt cccaaccatt gtctcagcta tcatcaccca ggggttgctg tgaaggaata
    31621 ttcccccgtt ctctttagtc ccgggcggat atgtagtaat gccgcctttt tccgggtcat
    31681 agcctttgta tcccggatag cttagtttta ttccgtttct ggtattcaat atgctcctga
    31741 ctgagtcaag ggattttaaa gccctgtctt caccggcaaa gcctgatatg actgcccagg
    31801 attgggcatt cgcatatatt tgccctgagc tgttcacttt agagcctata gggtttcctt
    31861 catagtcaaa atagctgata taccattctc cgtcccagcc gcagctgttc acacgctccc
    31921 gcatcctttg ataacgaccc cggtacatct ccttaagctt tttctttccg aggaaatcca
    31981 taagctctat catctcagaa aggcacttcc cgtacaggca ggcggtaaaa accgattcag
    32041 ctcccgccgg gagatttacg gtatcgttcc agtctgcaaa accaagaagg ggcaatccgt
    32101 gccttcccaa gtcatttccg gtaaatccga ctgctctttc caggtgctca agtacagtgc
    32161 ctttctcaat cggcatcccg cttttgtcct tttcataaaa aggcactgtt ttattgagaa
    32221 aatccatatt tccggtttcc ttgagatagg cacagacagc aagtactatc cacagatggt
    32281 catcactgta atacttggac ctatcctcaa aatcccttga atctccttca tttgcaatca
    32341 ttgtaaccgg attgaactga tgcattgcag agccatttct tttctgcacc cggagaagct
    32401 tttctatcag ctctctgcct tcctcgggag cactgtccat tattcccatt acatcctggg
    32461 cgctgtccct gaagcctatt cccctggagc catagccaag ctgatacagg gacaggtacc
    32521 tggaccagtt tttcgtaata tagcattgtc tcgggttgtg tatgttcagc atggtattca
    32581 tagctttatc aggggtttct acctgcagct tcgaaagata ttcctcccag aagtatttca
    32641 atttttcaaa ttcctcattg accctttctt cacttctgaa gctttccatg ctctcttttt
    32701 cttctgtaat gctcctgcac tgtccaagct gggtgatgat ccggcgggat tctcccggct
    32761 ccaatatgcc gatatgatgc atcatcgcag ctatattatc gccccgaagg acttcatggt
    32821 tgccaagttc gctgttatta aggctcccgg ggctctccca ggtgccgtat tcgttatccc
    32881 cgagaaaata ccttctgtcc gtttcatagg aagatgctgc atggtttgaa gtgaagaagt
    32941 tgatccttgt gtccttgttc atgtatgcat attgtataag cgtcctgtat cctctatgtt
    33001 cctcaataca ttccgaacgc attgtctgag gcacccaatc ggcgttaatg agttgtttca
    33061 gcgcttcaaa atgagaatac tcaattaccg gtatgacatc aagttccacg ggtactgccc
    33121 ttagattggt tatcctgatg tcccttatct ccctgtttcc tcccaaaggt acgaaaacaa
    33181 gcgcctctgt ccttatatcg tagaattctg atacaatcct tgtgtagcct aatcccacat
    33241 ggcattcgta catctggtat tgatccaggg taggcacaaa atacggagaa aacaaggtgt
    33301 atgcattttt accccttatc cttacataca gcgtttcccc cttaaattcc gacgccggaa
    33361 gctgcggtat atattttatt attctgttta ctgcagggtc tcctttgcat atcaatgctc
    33421 ctcccgtatg gtcgataaat cctccgaagt ccaggctgcc tatataattt atccatttta
    33481 cgggagttct cggatcagta atgacatatt ccctgttaat atcattaaaa tacccatact
    33541 gcataacaat tctcctttta tttcaacgcc cccggttgtt atacagctcc ggggcgatat
    33601 ttttccataa ctctgccgac taccaaatag atagcaaaag cagtaaagct gataaaaacc
    33661 aacaggaaca atccgggtac cgttcttgcg attaatgccg cggcagcaac aattgccaga
    33721 gttccgaagc aggagaacac atgctttatt cccaataccc aagaagcttt tattattcct
    33781 atggtgcttg attcatacct tgaaagcagt ggaaaaacaa aaaccgtaat tattaccacc
    33841 tgaaccagta ccggcagctg aactgccagc atcaatccgc ccatggagcc atatttctcc
    33901 aggaagctga ggttggtgta tatcatgaca tatgccgcac atagtatcat ccatatgcag
    33961 gtagcctgtt tgaaattttc cctgaagctt ttataaaagt ccttgatttc atgtacttct
    34021 tcttttcttg cgatttttcc tataacgtag tacaaagcag tagttgatgc tcccactgtt
    34081 atcagcggta tgctggttat gcaccagagc aggttaagta taatcatctg gtatactgcc
    34141 gtcattattt tataaatgat tccctcggta ttgaacaacc atttcataaa taccgctcct
    34201 tctgagactt gttttatgct taaggctaat actccatagg agcatcagcc ttaagcttat
    34261 gagtatctat tgcgtcagtc tatttctttc ccattttttc agcccattca ttaagctgtt
    34321 cctgcttttc agctattact ttgtcaatcc ctgctgcctt cagctcttca aggaacttcg
    34381 gcacatttac ttcagggtct attgagcctg tttcaagagc cggtacatat ttatcccaca
    34441 ggttgttcac tgctgcaatt tcatttttta ccggcgaggg gtcaaagtta aagcccaggg
    34501 tttgagttcc cgcagcctgc tgattgaatt cctcaaactg ctcccacttc ttcgggtctt
    34561 cgctctcata caggtaattg atgaactggt tgccgaacat ccagcctgtt cccggattat
    34621 atttcgtctg gtccggaccg gcctttatga cgctgtcact gactttaacg tagtgcttgc
    34681 cttcgatacc gaagtttata aggttgttga ggtatgggtc tgtgttaaag agttcaagga
    34741 acatcagtgc cctttcagga ttctttgacg tacttgagat agcctgtaag gagcccattg
    34801 tatccctgct tgaaattaca ggcggggtta tatatacctg tacccactca aatccgtgtg
    34861 taagtgacat ctccgcatct tttcccggct ttagggattt ggttgcagca aatattttgc
    34921 ctgctgcttc atcggcattg tagtcggtta cagtagcagc atccttcctg atatagcctg
    34981 cctggaagaa tttccttata gtcttaagca gtgccattgt ttccggtttt tcaaattcat
    35041 tgaatactac aaggtccttt gaatcgttgt atacaactcc ggggcacttg tcatcaccta
    35101 taacatccca gtccataagc ttatacggcg attcgccgtt caatgcttca agcccgtata
    35161 cattgggttc tttttccttt attaccttta gcattggctc caaatcttca agagtcttta
    35221 ttgctgacag gtcaaagccg tatttgtcta ccagatcctt tttgattatg aagccccagg
    35281 agtgagcctt ttccttgttt gcaggtatgc cgtagttgat tccgtctatc tgcgagcctg
    35341 caaggaaatc ttcacccagc aaagcctttg tctttggagc atacttgctt agcaaatcct
    35401 tctcgtcatt cagcggtaca aaagcacctt tcacggcgtt ctgcatgtag ttgtttgccc
    35461 aggctgcagt aaagcatatg tcaaactcct gccttgaagc aatcatctgc ctcagtttgt
    35521 cgtcatactc tgctccccac tggtagcact gaagctttac agtagcattg atcttgtctt
    35581 tgatatactc gttgatcttt tcctcgatca gggatacatc ctcctgctga ccattgccta
    35641 taaagaacca ggtcagttca acaggttcca atgtagttcc cgaacccgct tccgccggtt
    35701 caggtgcagg agccgcagcc ggctttgcac agcctgttag gctggccagc gcaaatacca
    35761 gcaccagtgt aagacataga agttttccgg ttttaaacat actcttctcc cctttaataa
    35821 atttttatat gcttgcctgt taaggcataa gcccttatga ttaacccttg attgatccga
    35881 tggtcaggcc ttctacaaaa tacttctgga agaaggggta ggccaatatt atggggccta
    35941 ttgcaactac agccatagcc attcttgctg tctgagacgg caggttcgcc atgatctggg
    36001 ttgcattgtt tatagagcct gcattatttg aaaggtatat ta
//
LOCUS       DAOQUR010000021        35273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1396_length_35273_cov_9.775938, whole genome shotgun sequence.
ACCESSION   DAOQUR010000021 DAOQUR010000000
VERSION     DAOQUR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 35273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35273
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_35273_cov_9.775938"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="PKI50_06220"
     CDS             complement(<1..97)
                     /locus_tag="PKI50_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNT03342.1"
                     /translation="MPSWKELKRFCENDGWELYKTTDHYFYRKIDA"
     gene            complement(76..501)
                     /locus_tag="PKI50_06225"
     CDS             complement(76..501)
                     /locus_tag="PKI50_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03343.1"
                     /translation="MEAINATDVRKDWGRFIDSVVRNKPKFIKRSRDYIFAASTDMLN
                     EMLKEYTLTATVYEEENGTITAELEEIDIAANGSTEEEALDILASDLQEYAEEYYNEF
                     EYWYNAPNRKAHLPYVLKVLLQEETKDILSMIKCQAGKS"
     gene            complement(736..1311)
                     /locus_tag="PKI50_06230"
     CDS             complement(736..1311)
                     /locus_tag="PKI50_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HNT03344.1"
                     /translation="MWIYEKKLQFPAWVEGCDLHIAKLLFAQYGGPDSELSAGLRYLS
                     QRYTMPIPEAKAVLTDIGTEELAHWEIIATLIFKIVDRATPEELIEAGMGPNYIQHGR
                     DVYPNDAAGVPWTAAYIQAIEDPITNLHEDLAAEQKARTTYEHLINSTKDRGVIETLS
                     FLREREVVHFQRFGETLRLVQEHMDNIKHKR"
     gene            complement(1315..1587)
                     /locus_tag="PKI50_06235"
     CDS             complement(1315..1587)
                     /locus_tag="PKI50_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HNT03345.1"
                     /translation="MDISRDRLELLKEIMAIDFSLVELNLYLDTHPMDQRALRLHNEY
                     VMKSRMLKEKYEKEYALITHKFISDCPWEWIEDPWPWEIDYEMGGR"
     gene            complement(1607..1789)
                     /locus_tag="PKI50_06240"
     CDS             complement(1607..1789)
                     /locus_tag="PKI50_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HNT03346.1"
                     /translation="MLVAPHYEIPVMGPEFKLAVAYIPYQVLQKVYEPMKGLMRGTIF
                     PELYRPYVKMKKDRDD"
     gene            complement(1980..3068)
                     /locus_tag="PKI50_06245"
     CDS             complement(1980..3068)
                     /locus_tag="PKI50_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03347.1"
                     /translation="MQDREEFKKVLLMQHERYPGMQIADMVKLIYQNEFAGGHLITNE
                     DECLKRLTEELRILEKDSGAYIPPVPFENIGNGLCRFYLVSEQISGIDIGTLNRFFIS
                     TSNSIRGNVQSFEQKLKVFKECCADGDFPYKPEEAEAWLDEYRRRGYPPMHHSGEYKA
                     LYSPAYRIVKLDYIHYLEIFRKIDSLLKSRDRVLAAIDGNCGAGKSTLAALIGDVYDC
                     NIFHMDHFYLRPGQGTRERMEEAGGNVDYERFREEVIDGILRGSEFKYRIYDCKKGVM
                     DEYITVLPKKLNIIEGAYSMHPALIDYYDLKIFLGIDEKTQRDRILERNGEVILEQFL
                     TKWIPMENRYFEQLKIEEKCDLVYRNNR"
     gene            complement(3058..3594)
                     /locus_tag="PKI50_06250"
     CDS             complement(3058..3594)
                     /locus_tag="PKI50_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNT03348.1"
                     /translation="MERLSIKNLVLSGFFIAFGLVLPFLTSQIPSLGSKFLPMHLPVL
                     IAGFVCGWQYGLIVGFIVPVFRSMLFGMPPMFPTAVAMAAELAVYGCMTGLMYKLLPK
                     RDAYIYAALLISMICGRIAWGLAGVFLYGLGGTSFTWEVFMAGAFINAVPGIIIQLIL
                     IPIIIIALKKAKVMQNAG"
     gene            complement(3779..4390)
                     /locus_tag="PKI50_06255"
     CDS             complement(3779..4390)
                     /locus_tag="PKI50_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HNT03349.1"
                     /translation="MIDSGISIDARAANSFYDRGRAKAYIDAYWKNYNPAYPSFSHGG
                     GDCTNFVSQVLYAGGMPWADDKNPANHKKSINWYCKPGATAKDSENRITFSWKIAAVF
                     KAHWISRAARHRILSYTEAMQSINWLSDSVFPGDVVQFCYSGGAPYHTLAVTGYNRDP
                     EYNIRDIVLASHDMDSNTRSLYRTMLKYPSDYKLRVYNIKLGN"
     gene            complement(4482..4656)
                     /gene="ssrS"
                     /locus_tag="PKI50_06260"
     ncRNA           complement(4482..4656)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKI50_06260"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4745..4915)
                     /locus_tag="PKI50_06265"
     CDS             complement(4745..4915)
                     /locus_tag="PKI50_06265"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HNT03350.1"
                     /translation="MEEINEVLMKIEELRKKMNDMIYESDDLLDSKIIELSRLLDEQL
                     TEYYRLLKKKNE"
     gene            complement(5138..6364)
                     /locus_tag="PKI50_06270"
     CDS             complement(5138..6364)
                     /locus_tag="PKI50_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNT03351.1"
                     /translation="MSEIINNREYRQKVLKELIMELHAGKTVEEVKSRFEELIKGVSA
                     SEISEMEQALIMEGMPVEEIQRLCDVHAAVFKGSIEDIHKVRKPDETPGHPVHTFKLE
                     NRELEKLINERLKPHLAGFRASDNSENIYKLLEDINLLLDIDKHYSRKENLLFPYMEK
                     YGITAPPKVMWGVDDEIRAAIKEIKGMLQDYKGEKEQVYTKADEAAGRVIEMIFKEEN
                     ILFPMVLETLTEDEWMTIESESDEIGYCLTEPRGKWKPERTDVEEKQKETGQTAADGY
                     LKFETGLLTAKEISLMFNSLPIDITFVDKDGAVKYFSQGKERIFPRTKAIIGRQVHNC
                     HPPASVHIVEKLVEDFKTGKKDSESFWIKMGGRYVYIRYFAIRDDNGDYAGTMEVTQD
                     IQPIQEISGEKRLVSD"
     gene            complement(6377..6604)
                     /locus_tag="PKI50_06275"
     CDS             complement(6377..6604)
                     /locus_tag="PKI50_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNT03352.1"
                     /translation="MSGEIDLSKTVYELSREYPEIVDIMKELGFESITNEGMLNTAGR
                     FMTIPKGAAMKGISMEKISQAFESKGYSIKG"
     gene            complement(6677..7366)
                     /locus_tag="PKI50_06280"
     CDS             complement(6677..7366)
                     /locus_tag="PKI50_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNT03353.1"
                     /translation="MKIVYTGKTKNVYALEDGNYLLKFKDDVTGTDGVFDPGSNTVGL
                     TIEGAGRAGLRLTKYFYEKLNSLGIPTHYIDADIEAGTMTVKKASVFGKGLEFVCRFK
                     AVGSFLRRYGKYAEEGQDLDAFVEVTLKDDEREDPPITEDALEMLGILSKDEYKVLKD
                     LTQKIAGIIKDELAKKGLELYDIKFEFGRTGEDKQITLIDEISGGNMRAYKNGSPVAP
                     LEIERLMLDAD"
     gene            7566..8612
                     /locus_tag="PKI50_06285"
     CDS             7566..8612
                     /locus_tag="PKI50_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT03354.1"
                     /translation="MKLKVLWTMAEGMGTASDLEMLKNAKPFDIDVVTAGTKGKDAAG
                     FVITGKKFVVPEVKDFKYLNAILDICRIEKVTTVIPQYTDELLLMSENTKQFNDLGIK
                     VLVTEDADKLRIANSKSELYKYFRQKSFVPEYTLASDIDSIKKAVYELGYPSVPVCIK
                     PVQGEGGKGFRIITDEKIDIFKEPVNSPKLSLEAYLESIKISDSIPELLVTEYLPGKE
                     YSVDCVCKNGTTYICIPRQRIETAMGVSSVSLIEKNEELISYSKEIISSLSLSYNINI
                     QFKYSSEGKPKLIEINPRVSGSLVANYGAGINMLEAALRLAYDMPLENIEIKWGTKMI
                     RQWREIFIQQHPKP"
     gene            complement(8602..9423)
                     /locus_tag="PKI50_06290"
     CDS             complement(8602..9423)
                     /locus_tag="PKI50_06290"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNT03355.1"
                     /translation="MNFRGITLGDKRIFGKFGYICSDYQFSYLYMYNNLYKLKIAESN
                     GAVIIRSDMAPACFYMPLGDTAQGIEAVIKYCNKMGTSPVFSKIPQEYTGVFKNFGFV
                     LEEDRDSFDYIFRKSDFIEYEGKMFRNQRNNLSSYVRAVMPEFDERIELYIDKCKAFT
                     LKYHNTEDKLEPTYKMLDSIAQFNLKGGVVLENDEVAAFCLYEKVSGDMAQSHVELTN
                     NSHRGVHTYLVSELAKRMDEEYINKEDDMGLPGLRRFKEKYNPCTMLKKYKAIMV"
     gene            complement(9551..10435)
                     /locus_tag="PKI50_06295"
     CDS             complement(9551..10435)
                     /locus_tag="PKI50_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT03356.1"
                     /translation="MNFRDFLANLQSVRDYEEENLKDLVLSDIRVYLEKINADAGKEK
                     GFSLIMLKNGSDVFNALKGVGGYSGVMIKSPHYIGLAVTKENFEDEFFGAYHMQSVVK
                     KLQEMYLGSCWIDIRDVSGDIKAKLSGDSESRINYLLAFGIPDEKAIKNKKPRTSVTN
                     EASEYRQNPYGIKYADAEITDRARLALGEIVYLHEWGRPAAYEELESRGMADLFFYIR
                     NAPSYKNLQPCRIILKDGEAELTVLEPQNPRNYIDAGIMMYTMEGLAKDLGIPGKWSF
                     VGPKAGNKGYGIAAHIEL"
     gene            10722..11081
                     /locus_tag="PKI50_06300"
     CDS             10722..11081
                     /locus_tag="PKI50_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNT03357.1"
                     /translation="MLEKKIWAVIGANNDSDKFGNIIYKKLKCEGYKVYPVNPMCEYV
                     DGNRCYPNLSSLPEMPEVLDMVVSPEKGKAFIEEAAALGIKNIWLQPGTCNREILELL
                     KAKQLTGVQACVLVALC"
     gene            complement(11089..12306)
                     /locus_tag="PKI50_06305"
     CDS             complement(11089..12306)
                     /locus_tag="PKI50_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNT03358.1"
                     /translation="MQEFFAVIGSFLIIPVLSRKKVPLGIAICACAVLMALLGGLGAF
                     DFANVISNTFFNFNKVQQLLVVAEFSIIGVLLKKYKIIDEVLDNLTKVIKNEKAILML
                     IPALIGMLTVPGGAIMSVPLVDQLGERSNISKPHRAIINLVFRHIPMNILPYATSFLV
                     VASISPRISIYKLIGLNSIFMMMYFIAAYYIYIRKAQSSPSPHYSFEWINLVKLLKFT
                     SPIYVAVLLNLLLGVPFYLGVLVNLLIVYLIHPTKTFLMDSLRAFSFNVLYALIGVYL
                     IQGIMGRMESLNSFLASILMNPNTIILGIIAASMFFGITTGFSPTALGVILPILITLP
                     LSDNMLLMYCHFTFCWAFVGYFFSPMHLCQIFTCEYLKVSIGDLYKDYWKFFVSLATV
                     LVLNYFVMGLFLR"
     gene            complement(12344..13591)
                     /locus_tag="PKI50_06310"
     CDS             complement(12344..13591)
                     /locus_tag="PKI50_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03359.1"
                     /translation="MKKNKDIRVGIGFATGRRSFQKVLKTYVYNWSESHLTGGEDVSL
                     NLLVAYDLKYKNTRSTDYTNVSEEVLELIDDAYFIGSNAIKQEIGYLIRGNVINENEA
                     KLFFGSGYAAKRNAVLYTAMKNRIDYLVFLDDDEYPMAVTNTRSTAIWGGQQVLSTHL
                     EHISQADITNGHHCGYISPIPYIEFNDTMTETDFRLFIEAISNDIINWESIRSIMNNG
                     GVTYADTKILVSDKAYEVQEVNHTKFISGANLCINLTNPDRVSSFYNPPGARGEDTFL
                     STCLSQRKVLRVPCYTFHDGFSTYNHLMDGVLPIKLKYIKADSEQIVNRFYRACIGWV
                     RYKPLLLYITQRENYENKIMEMREKLSDTLPKICDYFKRKDFMNISKELERYNKNVER
                     HYKQFIETQEIWKKIIMFLTQQQ"
     gene            complement(13733..15133)
                     /locus_tag="PKI50_06315"
     CDS             complement(13733..15133)
                     /locus_tag="PKI50_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNT03360.1"
                     /translation="MAKNQQIIGYLPDEQPPIWKLFLYALQQVVVMFPATVAVAIITG
                     FHISTTIFASGLATICFILVTKREIPLYYGSSFSYVAAIGSLMASDALAGYTLNEKIA
                     VAQFGIIMSGFVSIAAGLIIKRFGRDAIEKVLPATITGPIAMVIGLTLSGLAMSDAAS
                     IAIDTAGTISVAESQLAFAGSMAWIISLVTLISTILLSVYLKGFLGQLPLLIGPIIGC
                     FTAFIIKLATGIDLFKVLPDTANSGIFALPIFTFPKASLMAVAAIMPIAIATIPESTA
                     HVYQLDIYVNDLAKKKGSDKRYNIADKLGLNLIGDGIGDMVSGFIGGPAGTNYGENIS
                     AMAITRVFSIPVLMGASVIAMIIACFTPLVNAIYSIPTSVIGGLEVYLFGAIAAQGIA
                     IMVEKKVDLFSSKNIAIIATIMIIGLGGQFGFGGNIPFFGMQVPCIAGAAIFGILLNL
                     VLSIGEKKNTKKPEEH"
     gene            15489..16487
                     /locus_tag="PKI50_06320"
     CDS             15489..16487
                     /locus_tag="PKI50_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNT03361.1"
                     /translation="MSYLLKPLKNGKLLLKNRLIMPPMATAKCEKDGKPSKALFDYYD
                     EKSRGGYISLIIVEHSYISQQGKASDRQLSAADDSLIDSLKELAGIIHKNGTKAVMQI
                     NHSGSAAKYEVTGMDAVAPSAVSNPRHNGPLPKELTLKEIKAIIEEFRAAAYRVKMAG
                     FDGVEIHSAHGYFLNQFLSPLANKRTDEYGGNIHGRIKIHLEVIKAVREAVGEDFPIF
                     LRLGASDYMEGGTSIEDSKIAAMEFEKSGVDIIDISGGFCGYIGRGTAEQGYFSELSE
                     AIKKVVSVPVILTGGVTEAQAAEKLLADGKADLIGVGRAILNDSDWAKKAVESLKP"
     gene            complement(16557..17195)
                     /locus_tag="PKI50_06325"
     CDS             complement(16557..17195)
                     /locus_tag="PKI50_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT03362.1"
                     /translation="MYIRNRMTPNPCTIPSNTTIADAVALFREKGFKRFPVVDNGKLV
                     GILTKGDIQSVSPTKATSLSIYEVNYFLSKITVRKAMTKKVITIDADCLLEEAAVAMR
                     ENKISTLPVMENGKLVGIITESDIFDAFIDLMGFKEKGSRITVEVKDVPGGIAAISEV
                     FSSLHINITHIAMFAGKGIRDLIIRSSVEDVSEIEKKLNEKGYKIKHLLINK"
     assembly_gap    17588..17687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17688..17763)
                     /locus_tag="PKI50_06330"
     tRNA            complement(17688..17763)
                     /locus_tag="PKI50_06330"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17728..17730),aa:Ala,seq:ggc)
     gene            complement(17869..19767)
                     /locus_tag="PKI50_06335"
     CDS             complement(17869..19767)
                     /locus_tag="PKI50_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT03363.1"
                     /translation="MKEKTRYIAIAAAVLLALVLFSGMTVCAGSEDTDEGYKDGYSAG
                     WTDGMEAAWEDLDEDRRKSYSKAIPSNSEIKEYYELDEESNDYEDGFLEGYRDGFREG
                     YNFGYENPESGAASQVNYDRELGYSMGEIRGRLDYWEGLENSWNLHVPKTIEIIQLFG
                     LKMESEAYKNDFITEFRVNYKKGYEYGYRMAKFEPMKTAMEQGVKDGELFGGIMGAGR
                     GRLDYYSGSVSQWDRKLPSDSSIKAIFLLPKDSEGYGNAFLAAFKSAYRTKYEEAYRK
                     ANIEKNLLIFERGYEQGREIGVIRGASLARIDRTMNLTNYDTRKNYNDQDIIKEYMLF
                     NEDEKYREGFIGGFREGLKEGYITEYRDSNYEYGKRKIVTETIPISGAEIFSGDRLMK
                     LTIEKGTFYNIAAVSIDKTVTSNANIKLPSKEGMIKASEFYTISVANSSSSVNPDKSA
                     ELSFEYYGPANGGIYKYSNGTWIYLPSRINGNSISTKLSPGSINNRTCTYGVFIDENA
                     RDPYDLRGNWAKDEIAAYLRRGIAEAYPDGSFRPDSPLTRGQAAAWINNVYKTKLPAP
                     ETPNNTVTYTEIEKLMKQATGDENFKWSYIAEKMALNKDKRSGSFSSVSGIVTRAEAI
                     YMLYYLNE"
     gene            complement(19786..21150)
                     /locus_tag="PKI50_06340"
     CDS             complement(19786..21150)
                     /locus_tag="PKI50_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT03364.1"
                     /translation="MELKLGNNKPGSGLNISTKEKSLLLLLAVVTMLWLFHKYVITPQ
                     ELEIQRLKAEKASYEEEYMKMREILSKESTIRKEYAALLEDYGKTAGKYYSGDEQPEL
                     MYMINGIIDRSKLNIPGISFRETETAELEGIDAKLSGVSLAYRGSYSDLEKLLSQLRN
                     NPRRVLIEELSIHREDAETLSGQVAFSAVTFGGKETEKDGYFYANEFTAQGKADPFEA
                     FKGYAEAAEGNSGSWDEEKRLLLSDLESDAVYFMSIGSGVTGAVEKIPEGKFGRTSVR
                     AEYYISAGYQPERAYVVLDDQNINIKYPPSSIGIWAYSYGYSPVTVGLRFQSQEGEKI
                     DLKLSEGVNWVGWKYISASPTQDVNVYPLKLDRIYIELGPEKDDYGVLLFDRIEAEYT
                     DNEESAENQDSFEFYVVQPGDTLRGISEKFYGTGSRYTSIAKDNGLPKNSAPEAGRIL
                     VIRK"
     gene            complement(21125..21706)
                     /locus_tag="PKI50_06345"
     CDS             complement(21125..21706)
                     /locus_tag="PKI50_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNT03365.1"
                     /translation="MNDMNFFSPYNSKRAKKDIEKPVIFIKGLAVLVLIGIIAYGAFN
                     HLSIRRLGKEINALNTELEAVRKDPRLEGIMAMEKEAALLKEDLSGLYALDKYIESRD
                     TINEFLLEAIRVNTPAELFLDAMQMNQHSIKLEGKSKDKESIALFEHNLREDEGLEKV
                     FVSRVTNENEYYSFYMDIELKGEMPGGAETGKQ"
     gene            complement(21711..22796)
                     /gene="pilM"
                     /locus_tag="PKI50_06350"
     CDS             complement(21711..22796)
                     /gene="pilM"
                     /locus_tag="PKI50_06350"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNT03366.1"
                     /translation="MEGGIRLKVFSFLGSSITAVDLGSYETKIIAGKQEREGISVKKA
                     FSFLTPPNTYDNGYIEDGFKLAAVVREELAKNNISGGTCHITVKSTSILTREILLPRL
                     AGKDIEGLLHYQMEEHLPMDFSKYVIQHKAIERITEDGKEKLNVLVAAVPKELVETHY
                     DFVRKLGLKPEVMDYQSNSIWKFLNHAGSVNGSLYPQDKTIAAIDLGYSSSNVTIISE
                     GKAKLNRAIDSGGYSLDKNISSLTGLSGGEIKACKQGIIDVSTVQEGYSEQNRYTNIV
                     KTSIEGIMDKIDKVFKYYASKEPEKRLEVLLLYGGLSRIRGIDNLFSGYFNIPALVLN
                     STSRIYPNGDINAYVNCAGALIRDNGV"
     gene            complement(22775..24391)
                     /locus_tag="PKI50_06355"
     CDS             complement(22775..24391)
                     /locus_tag="PKI50_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03367.1"
                     /translation="MRWFRNNKGAALPIVIIVLAISSIFGFFSLSMLDSQARFNIMDD
                     SGKKAMEYAEAGYNAYLWHLNDNVEFYSTKESDDMQGVPIEFMDGYYQLSVTRPSDTD
                     RFITIVSTGWTKDNPGIKRTIEAKIRKKQFVHHVYVSNDDGSSIWWTSGDESHGPYHT
                     NKNLYTREGASIYNTTLKKTVYYPVFFDTVSYSGTHTENIRSSYRDDAYLAGNPSKTD
                     ILEFPATNSQLKAWAEKDNMVFYGRTCIYLDGEYIKIRNGNASAAEIQTYKISDIYNK
                     VIYVDKAAGGGTGKFDLESGNIFISGTIKGVLTIAAANDIYITYGDPTNWYDYSAEDL
                     TRRDPAPNQPPVSFRWSDGRNYDYPEYGSIEYESTTFTSSHKPGGKEYYIREANGKDM
                     LGLVANRDISILHFNWPKMSVGGDEPCWDFVWKWNNRTKRYVKENYKYDVAPKDVTIH
                     SALFAINGGFGFERYDERNGESKYVRKGDITIWGSITQRTRRPVGIIDRTGYNKKYSH
                     DPRMFCDYPPHILEPVNVGWEIHEWKEVSD"
     gene            complement(24408..25022)
                     /locus_tag="PKI50_06360"
     CDS             complement(24408..25022)
                     /locus_tag="PKI50_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03368.1"
                     /translation="MNGRVKNSIYNRQGFVLVELVISIPLIAMVFLLGYNMFFLAQNS
                     LSCVSRTVDVTEELRFFQKNIYSEANRAKKAEEKKDALHRISSTEIYIYTDVDNEDGR
                     NIPEIVRYRIVSGNLQRDVKKPQGTEYPYTYTSDFGTAEIVLRNISNTDIFSEQEPIR
                     IVSEDSQESPDYRRKLRMRLVIEGEPNDIEVQGYLISKSRTEAE"
     gene            complement(25039..26457)
                     /locus_tag="PKI50_06365"
     CDS             complement(25039..26457)
                     /locus_tag="PKI50_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT03369.1"
                     /translation="MRMRRDKGFTIIEIIVSIALLGMISLFLLPLSAYSIQHSRGNNI
                     RLTAINLAYTQVEWLKTLDYNSELGLDSAGYSPSGKVKENLYLNETGSDPKVIDGIEY
                     RLMTSIYWESAVSSQGEYIANAAKKADVTVRVRDPLSGKEKAFSVLGTLIAFEGERTL
                     YSYVPLKVRTITGEDFTKPAKNVKVTVKNLSNTVVSWSRTDEKGEALFAGLANAKYNV
                     CPEEWEKGEMTARPTGTSGAYPNEDWIYHLQVEVKATGDDFLEQIFYVDYPAYIVLTG
                     YPKALTDNSRVRLDPIYDQPEGDTAAYDLDTNLTSLMTKKIWRAWDYGHSITSGAYSY
                     YFVDKDTGNLWDGSFDYMENGVTRKELKLAFGLRDGSFRREADGSITLTVEFTSEING
                     IDTLEFSLYDGDTPITCTDITITQAVSGMNNKFLINLRASAGITGNKLRFEADNKEAD
                     TLVNEYGMKLVQDRNYCILTED"
     gene            complement(26469..27230)
                     /locus_tag="PKI50_06370"
     CDS             complement(26469..27230)
                     /locus_tag="PKI50_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNT03370.1"
                     /translation="MYIPVFIMGITIGSFLNVCIYRIPRGESVVSPPSRCTRCSHSLA
                     WYDLMPILSYIFLKGKCRYCGEGISPRYPIIEALNGILYLFIFYYFGLSFEFYFYCFM
                     ASILIVICLIDYYERIIPDGLVLAILAATVLYKGLGHFILGTPLLFRDSIYGFLSGGL
                     FFLFIAVVSKGAMGGGDIKLTAALGLILGFKKTVLNILLSFIIGALVSLYLLISGRKG
                     RKDEIPFGPFINISFFITLFFGDGIIYRYLQSFTG"
     gene            complement(27270..27656)
                     /locus_tag="PKI50_06375"
     CDS             complement(27270..27656)
                     /locus_tag="PKI50_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT03371.1"
                     /translation="MLQWFLKKVRNRKGFTLVELVVVIAILGILAAVAVPKYTVSRKE
                     AADTVNDTNKKILQSAASMAVADGTDAVTWDKDTGETTDGTKLGWSNYLQEWPEVPKG
                     ATESGTYKVVIDGDGKVTVSVEGAGD"
     gene            complement(27751..28953)
                     /locus_tag="PKI50_06380"
     CDS             complement(27751..28953)
                     /locus_tag="PKI50_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT03372.1"
                     /translation="MQFKYRAVAENGQIIEGLQDAKDEQELVAMLKSSSYMPISVGKT
                     AGSGGSMEVTFAKVRKKDMAVFCRQFHTMLNAGVSVVKCLDILERQTENNLLRKAINN
                     AWENVQKGMTLSEALKENSRVFPSILISMVEAGEVSGTLDTIMERMSAHFEKEFKIEN
                     KVKGAMIYPIVLAGVATAVVLFMMIAVLPGFIQMFESSGIRLPLPTRILLAVSHTIIR
                     RWYVYVLAASGLFTGLRYFGRSEFGRQLFDKWKLRLPVIKNTNIKIAASKFSRTLSTI
                     LSSGIPLVQGIELVSRVMDNKYIGEKMESVKEGVRKGLSLSETIKEIGVFPPMMDAMI
                     KIGEESGDLDGILAKTAGFYDEEVEVAIQKMMEMMQPILIVVMALIIGFIVMSIALPM
                     FDMINMSG"
     gene            complement(28980..30023)
                     /locus_tag="PKI50_06385"
     CDS             complement(28980..30023)
                     /locus_tag="PKI50_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT03373.1"
                     /translation="MDFIELINDGVGKKASDIHITVGMPAIYRINGGLVKAGDSVLTP
                     EETEKMAMSVLNDRQKEVLKEKGEIDISFSIQGVGRYRINIFRQRGSHCMAIRVIPLN
                     IPSMEELGLPMIVNDLTELRNGLILVTGPTGSGKSTTLAYMIDRINREKSCHILTLED
                     PIEYLHRHKKSIVNQREMGSDSMSYANGLRAALRQDPDVILIGEMRDLETIGIALTAA
                     ETGHLVLSTLHTVDAANTVDRVIDVFPSDQQRQIRVQFASVIQATISQQLLPRADGNG
                     QACAFEIMMATEAVRNLIREGKTHQLSTVIQTGGKYGMQTLDSSMMNLYRRNLISRDT
                     LMTRSMNPDMIRL"
     gene            complement(30035..31720)
                     /locus_tag="PKI50_06390"
     CDS             complement(30035..31720)
                     /locus_tag="PKI50_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT03374.1"
                     /translation="MKTSLRLGDMLLHSGKLSRQQLEEAINIQKSSKTTKKLGEILVE
                     IGYTSEEEILGVIEEQLGIPVLDLANYIINPKAAELIPEAIAKKYDLMPVDIVDNELL
                     VAMSDPLNIFAIDDIKLISGMKPKIAISTKSVISRTINKYYTRESSKKIIREFEEGFE
                     PGRQEELDENELSDVNSAPVVKLVNTIISQAVRNKASDIHIEPYEDIIRVRLRIDGDL
                     QEIMSLGRRSHMAIVTRIKIMGKMDIAERRLPQDGRVEMESEGRNIDLRISSLPTVYG
                     EKIVIRILDRDGFMFSKEDLGFSDHDLEVFNRIIHHPYGIILATGPTGSGKSTTLYTV
                     LKDINKEDRNIITIEDPVEYKIHGINQVQVNNKAGLTFANGLRSILRQDPDIIMVGEI
                     RDSETAEIAVRASITGHMVLSTLHTNDTASTVARLMDMGIEPYMVSTSVVGIISQRLV
                     KRLCPSCKEAYRADEKEKELLKMDRGKELILYKPKGCNKCIKGYRERVPIHEVMYLDK
                     DIRRLIDKRGSTEDIKVQAIKNGMTTLQDSVARIALEGITSMEEVLRIGHTID"
     regulatory      complement(31759..31839)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            32095..32715
                     /locus_tag="PKI50_06395"
     CDS             32095..32715
                     /locus_tag="PKI50_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03375.1"
                     /translation="MKKQKTLRRDRINRTLESIFEYPLTVVEAPIGYGKTTAVREFLA
                     SKVCFVLWLSFLSGEDTSTFFWDRFSEEICRLDEEAGMKLKLLGFPVDAPQTATVLSV
                     LNEIEFKENTVLVIDDFHLVKGHQIGAFLYQLVKNQPDNLYITIVTRDTSNLDLLELA
                     AKGLCNIVTQQVLRFTDDEVRDYCVIRGCRRLQYSQHTAGFSENNA"
     gene            32645..33409
                     /locus_tag="PKI50_06400"
     CDS             32645..33409
                     /locus_tag="PKI50_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03376.1"
                     /translation="MLLGAAGVYSIHSILLDFLKTMLKDEQKRKALWCRMGEWYLSKG
                     AHIPACSYLCRGGDTMRILSLLNDINKIAYNSPSFEGFSEMFAAIPRDTLFKYPYAYM
                     QYIDILMISGDSAEAREGLARLNEFQDVYEHTEDISSDFKNRILGEISTMRIFSVFND
                     AEQMVSQMNEALRLLDGDVSYLIKRESEFTFGSPHLLYTYYREPGRLRETADYIVRKF
                     PFFAKLANGCGTGCDYLTMAEYALETGDWQSAEKCL"
     gene            complement(33547..35094)
                     /locus_tag="PKI50_06405"
     CDS             complement(33547..35094)
                     /locus_tag="PKI50_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HNT03377.1"
                     /translation="MLGKNNPQIDVFTHMIFDRLIPEDHLLVLINSIIDFSFVYDIVK
                     EKYSDIGRKSEDPVMMLKICLLEFLYTLSDKKVVKRIQTDIAFRWFLGLSLDDEVPDD
                     TTISHFRTNRMGEESFDEFFNEIVRNCIEKNIVKSKRYLIDTTDVAANVNYPSNRRLI
                     CDAFRKVIRETRKFNELLAEQLLVQFENEIEEEYKIDDKVSVKEYLQIAQKYAEYLYL
                     KTYDELQTNEKYQEVFGILWDIIDQYTNGKKDKIVSVVDPDAKIAHKSLGNIKRGYKD
                     HIIVDEDSEIILASVQTPFNVGDEKKLAELIEKVEESLGLKPEEITADKVYGTTGNRA
                     YLKDNEIISNIAFYEESSREVKYYGLRDFKISEDLKSVTCPNGIITEEYVITRNKTND
                     TDFKVFKFDKAICQQCPLRDQCLYKDKNGKVVSKGRRLDVPLRYDAVLRDMKRVATKE
                     FEEASNKRFKVERRFATMVRNHGLRRCRYVRLGRAKIHITLANMACNIIRMVNLLCQP
                     EFAASPN"
BASE COUNT         9082 a         8525 c         6546 g        11020 t
ORIGIN      
        1 aagcatcaat ttttctataa aaataatggt cggttgtctt gtacaattcc cagccatcat
       61 tctcacaaaa ccttttcagc tctttccagc ttggcatttt atcatactca atatatcttt
      121 tgtttcttcc tgcaggagta ctttaaggac atagggcaga tgggcttttc tgttaggggc
      181 attgtaccag tattcaaatt cattatagta ttcttcagca tattcctgta agtccgaagc
      241 caggatgtcc aatgcttctt cttctgtact gccgtttgct gctatatcta tttcttcaag
      301 ctctgcagtt atagttccgt tttcctcttc atatacagtg gctgtaagtg tgtattcctt
      361 cagcatttca ttcagcatat cggtgcttgc agcaaagata taatctcttg agcgttttat
      421 aaattttggt ttgtttctga caacggaatc aataaatctt ccccagtcct tccttacatc
      481 tgttgcgttt atcgcttcca atttcttcat cccccttcgc ctcccattat aacatagaac
      541 gtacataatg tacagtatgt acgttcctat cttataatat tacctgcatg ctgtatttta
      601 ttcctgttat tacaagattt caatgagggt tttgatatat tcatctagtc aacactgttt
      661 ttgcctccta acacaataag tgtacaatta aattccctct actataaaaa aaagagggct
      721 gccgccctcc ttcagctatc tcttatgctt tatattgtcc atgtgctcct gcaccagcct
      781 cagagtttca ccgaacctct ggaagtgaac cacttccctc tcccttaaga agctcagtgt
      841 ctctattact cctctgtcct tggtggagtt gatcaaatgc tcgtaggttg ttcttgcctt
      901 ctgctctgct gccagatcct catgaaggtt tgttatggga tcttcaattg cctgtatata
      961 tgctgccgtc cagggtactc ctgcagcatc gtttggataa acatcacggc cgtgctgaat
     1021 atagttgggg cccattcccg cctcaatgag ttcttcaggt gttgctctgt caactatttt
     1081 gaaaataagt gtcgcaatta tctcccaatg agccagctct tcggtgccga tatcagtcag
     1141 cactgccttt gcctccggta taggcattgt atatctctgt gacaggtatc tcagaccggc
     1201 tgacagttcg ctgtccgggc cgccgtattg tgcaaaaagc agctttgcga tgtgcaggtc
     1261 gcagccctct acccaagccg ggaactgaag ctttttctca tatatccaca tatattatct
     1321 ccctcccatc tcataatcaa tttcccacgg ccagggatcc tctatccact cccagggaca
     1381 atcgctaatg aatttatggg ttatcagtgc gtattctttt tcatattttt ccttgagcat
     1441 cctggatttc atcacatatt cattatgcag cctgagtgct ctctgatcca ttggatgtgt
     1501 atccagatac agattcagtt ctaccagaga gaaatcaatc gccataattt ccttaagcag
     1561 ttcaagcctg tctctgctta tatccattac aaataccttc ctttcttcaa tcatctctgt
     1621 cctttttcat ctttacatac ggccggtaca attcgggaaa gattgtaccc ctcatcaagc
     1681 ccttcatagg ctcataaacc ttctgaagca cctggtaagg aatgtatgcg accgccagct
     1741 tgaattcggg acccataaca ggtatttcat agtgcggagc tacaagcatt tcaggtttgt
     1801 acatatgatc ataggtatag ccttttgcca tcattgaccc ccctttatac gttttccctt
     1861 ccatactatt catctgcctg ccgatatgtt acatgcagtg ggggctaatg caaaaaatat
     1921 aaaactcctc caggcaaaaa caaattcatg ccgtaaagga gcttcttgtt ctgtattttc
     1981 tatcgattat tcctgtaaac caaatcgcat ttttcttcta ttttaagctg ctcaaaatat
     2041 ctgttttcca taggtatcca cttggtcaaa aactgctcca gtattacttc accgtttctt
     2101 tccagtatcc tgtccctttg tgttttttca tcaattccaa ggaaaatttt taaatcatag
     2161 taatctatta aagccggatg catgctgtac gccccttcta ttatgttcag ctttttgggc
     2221 aggacggtta tgtattcatc cataaccccc ttcttgcagt catatatccg gtacttgaat
     2281 tcactgcccc ttagtattcc gtcaattact tcttccctga agcgttcata atccacattt
     2341 cctccggcct cctccatcct ttcccgggtt ccttgcccgg gcctcagata aaagtggtcc
     2401 atatggaaga tgttgcagtc gtatacgtca cctataagtg cggcaagggt gctttttccc
     2461 gctccgcagt taccgtcaat ggctgccagg actctgtcac gggatttcag cagggagtca
     2521 atttttcgga atatctctaa ataatgaata taatccagct tgactatacg gtatgccgga
     2581 gaatataatg ccttgtattc tccgctgtga tgcatcggcg gataaccccg ccttctatat
     2641 tcatccagcc aggcttccgc ttcctcaggc ttgtatggaa aatccccgtc agcacagcat
     2701 tccttgaata ccttcagttt ttgctcaaag ctttgaacat ttcctcttat agaatttgaa
     2761 gtgctgataa agaaccggtt caaggttcca atatcgatac cggagatttg ctccgacaca
     2821 agataaaacc tgcaaagtcc gtttcctata ttttcaaagg gaaccggcgg aatatatgca
     2881 ccggagtctt tttcaagaat ccggagttcc tccgtgagtc ttttaaggca ttcatcctca
     2941 tttgtaatca agtgcccgcc ggcaaattcg ttttgatata taagctttac catatccgca
     3001 atctgcattc cgggatatct ttcatgctgc atcagcaata ctttcttaaa ttcttcccta
     3061 tcctgcattc tgcattacct tagccttttt taaagctata ataattattg gaattaaaat
     3121 caattgaatt attatgcccg ggacagcgtt aataaacgca cctgccataa agacctccca
     3181 ggtaaaagaa gtaccgccaa gtccgtacag gaatactcca gcaagccccc aggctattct
     3241 tccgcagatc atggatatca gtaaagcagc atatatgtaa gcatctctct ttggtaacag
     3301 cttatacata agccctgtca tacagccata gacagccagc tcggcggcca tagcgacagc
     3361 tgtcgggaac atgggcggca tcccgaacag catacttctg aataccggca ctataaagcc
     3421 gactatcaag ccgtattgcc agccgcatac aaaacccgca atcagaacag gcaaatgcat
     3481 aggcagaaat ttgctgccca ggcttgggat ctgtgatgtc aggaatggca atacgaggcc
     3541 aaaagcgata aaaaaccctg aaagtacaag attcttgatt gataatcttt ccattttgtt
     3601 ttcctcctgg tgaaaaaata aaaagccatg caaataccgc tttcaatctt gaaagcaaat
     3661 accttcatgg tataaatcct aaaatatcat ccctgtttgt ttcagtaccg gcttctgccg
     3721 gcgattaaac ttagtttagc atatactgtc ctgctttaca agattcactt tcaaaacact
     3781 aattccccag cttaatatta tacaccctca gcttataatc cgaagggtat ttcagcatgg
     3841 tcctgtataa tgagcgggta ttcgaatcca tatcatgaga ggccagcact atatccctta
     3901 tattgtactc gggatccctg ttatatcctg ttacggccaa ggtatgatac ggagcacccc
     3961 cggaatagca gaattgcacc acatccccag gaaataccga gtcggacagc caatttatgc
     4021 tttgcatcgc ttctgtatag ctgagtattc tatgccttgc agcccggctg atccagtgag
     4081 ccttaaaaac tgcagctatc ttccatgaga aggttatccg gttctcgctg tctttagcag
     4141 tcgcaccggg cttgcagtac cagttgatac ttttcttatg atttgcaggg tttttatcat
     4201 ccgcccaggg catccctcct gcatacaaca cctgtgaaac aaaattcgta cagtctcccc
     4261 ctccgtggga aaaggatgga tatgcaggat tatagttttt ccagtatgca tctatataag
     4321 cctttgccct tcccctgtcg taaaagctgt ttgcagctct tgcatcaatg cttattccgc
     4381 tgtcaatcat tgaatccccc caaatcagcc cattgaatat taatgcccat aaattattat
     4441 attcctgcaa atgggaaata ataacctacg ggcatttcca tatagtcccc gaaatgccgt
     4501 cagccataat cttaaaacct gccatcagca ggtgggtgcc ctgattcctg cttcaatcct
     4561 ctggtctgtg ccagcccgat ttccacttta atacccgggc tccccattgt aaattgaggc
     4621 ttaagattac taaaccaacg aacatttcag gtacatttcc tgttacttaa attataaacc
     4681 tgtccgtgta aaaaatcaat gtgtcttcac cttgaataca ataattccat gaccggtaac
     4741 actgctattc attctttttc ttcagcaacc tgtagtattc agtcagttgt tcatccaaga
     4801 gcctgctgag ttcgattatc ttactatcca acaggtcatc actttcgtat atcatatcat
     4861 tcatcttttt tctaagctcc tcgattttca tcagcacttc attaatttct tccatttgta
     4921 tacacctttc aactgtatta atatcatgtt tgtgagtata gcgaactatt gtgacatatt
     4981 cagtatatta acccgtataa atacttaata aacagagaaa aaatggaagc atccgaaaat
     5041 gctcccattt caattcaaag gttcaaaatc tgcataatcg ttgaagttca tcagtcttgc
     5101 tctatggact ccatctccct gatgtttatg gtgcagttta atccgatacc aatctctttt
     5161 cgccggatat ttcctgaatc ggttgtatgt cctgggtcac ctccatagtg ccggcataat
     5221 ccccattatc atcccttatc gcgaaatacc ttatgtatac gtacctgccg cccatcttaa
     5281 tccagaagct ttcgctgtct tttttacccg tcttgaagtc ctccaccagc ttttcaacaa
     5341 tatggacact tgccggagga tggcagttat gcacctgcct ccctatgatt gccttagtcc
     5401 tggggaatat cctttctttt ccctgagaga agtatttaac cgctccgtcc ttatctacaa
     5461 aggttatatc aatgggaagg ctattgaaca taagactgat ttccttagct gtcaaaaggc
     5521 ctgtttcaaa ttttaaataa ccgtcggcag ctgtctgtcc cgtttctttc tgcttttctt
     5581 ccacatctgt cctctcgggc ttccacttgc cccggggttc tgtcaggcag tagcctatct
     5641 catcactttc actctcaatg gtcatccact cgtcctccgt taaggtctcc aataccatcg
     5701 ggaaaagtat attctcttcc ttgaatatca tttctatcac tctgcccgct gcctcatccg
     5761 cttttgtgta tacctgctcc ttctctccct tataatcttg cagcattccc ttaatttctt
     5821 ttattgctgc cctgatctca tcatccacac cccacattac tttgggggga gccgttatgc
     5881 cgtacttctc catatagggg aacagcagat tctccttcct gctgtaatgc ttatcgatat
     5941 caagaagcag gttgatatcc tccagcagct tgtatatgtt ttcactgttg tcggaagccc
     6001 tgaatcccgc caaatggggc ttcagcctct catttatgag cttttccagt tctctgtttt
     6061 caagcttgaa ggtatgcacc ggatgtcccg gtgtttcatc cggtttccgc accttgtgga
     6121 tatcctcaat ggagcctttg aagactgccg catgcacatc acaaagcctt tgtatctctt
     6181 ccacaggcat gccttccata atcagcgcct gctccatctc cgatatctct gaagccgata
     6241 cgccctttat caactcttca aaccttgatt ttacctcttc caccgtcttt cctgcatgga
     6301 gttccataat aagttccttc agaaccttct gcctgtattc cctgttgttt ataatttcac
     6361 tcatttttaa tgccccctat ccttttattg aatatccctt actctcaaaa gcttgactta
     6421 ttttttccat acttatgccc ttcattgcag cacctttggg aatagtcata aaacggcctg
     6481 cggtattcag catgccctca ttggtgatgc tctcaaaacc cagttccttc atgatgtcca
     6541 ctatttccgg atattcccgt gaaagctcat agacagtctt gctaaggtct atttccccgg
     6601 acatatagac atttcccctt tcaattattc aatttggata ttctgtgcat tttatgagaa
     6661 tgtcattttg ttttccttaa tctgcgtcca gcataagcct ttctatctcc aagggcgcaa
     6721 caggagaacc gtttttgtat gctctcatat tccctcccga tatttcatca atgagggtga
     6781 tttgcttatc ctccccggtt cttccgaatt cgaatttgat atcatatagc tccaatccct
     6841 ttttagccag ctcatccttg attattcccg caattttttg ggtcaggtcc tttaatacct
     6901 tatattcatc cttggataaa atccccagca tttccaacgc atcttctgtt atcggcgggt
     6961 cctccctctc atcatccttt agggtaacct ccacaaaggc atccaggtcc tgtccttcct
     7021 cagcatactt cccataacgc ctcaaaaagc ttcccaccgc tttaaaacgg catacaaact
     7081 ccaaaccttt tccgaatacc gaagcctttt ttacagtcat agtacctgcc tcaatatctg
     7141 cgtctatgta gtgtgtcgga attcccaggc tgttcagctt ctcatagaag tatttggtca
     7201 gcctgaggcc ggccctgcct gccccctcaa tagtaaggcc aacggtgttg gagccggggt
     7261 cgaacacacc atctgttccg gtgacatcat ccttgaattt cagcaggtaa tttccatctt
     7321 ccaaagcata tacgtttttg gttttccctg tataaacaat cttcataagc aagttcgttc
     7381 ctttcttata ctaatttcac agttgaagtt ccgatgttat gattaaaaag tatatcaaat
     7441 cattgaagga ggcacaagaa aaatatctta tcagctcact aatatatgct cccttgacta
     7501 tatatagctt gtaagatatc atcaaaaata tacaggattt cctgaattgg aagggaggta
     7561 tataattgaa gcttaaagta ttatggacaa tggcagaggg catgggaacc gcctctgatt
     7621 tggaaatgct taaaaatgcc aagcctttcg atatagatgt ggttactgca ggtacaaaag
     7681 gcaaagatgc tgcaggcttt gtaataacgg gtaaaaaatt cgtggttcct gaagtaaagg
     7741 actttaaata tttaaatgcc atactggaca tatgcaggat cgaaaaggtc actaccgtta
     7801 taccccaata cactgacgaa cttttgctga tgtctgagaa tacaaagcag ttcaatgatt
     7861 taggcataaa ggtactggtt actgaagacg ccgataagct tagaatcgca aacagcaaga
     7921 gtgaactata caaatatttc aggcaaaaaa gcttcgtacc ggaatacaca ttggcttctg
     7981 atatcgattc aataaaaaaa gccgtatacg aactgggata cccttctgtg cctgtatgta
     8041 taaagccggt acaaggggag gggggaaaag gattcagaat cataaccgat gaaaaaatcg
     8101 atatattcaa ggagcccgtt aattctccga agcttagcct tgaagcatat ttggaaagta
     8161 taaaaatttc ggacagcatt cctgagcttt tggttaccga gtatcttccg ggaaaagaat
     8221 acagtgttga ttgtgtatgc aaaaacggaa caacgtatat ttgcataccc agacagagaa
     8281 tagaaactgc aatgggagta tccagtgtat ccttgattga gaaaaatgag gaattaattt
     8341 catattcaaa ggagataata tcctccctca gcctttcata taacattaat atacaattca
     8401 agtattcaag cgaaggaaaa ccgaagctca ttgaaatcaa ccccagagta tccggttctt
     8461 tagttgcaaa ctacggggca gggataaata tgctggaagc tgctcttaga cttgcttatg
     8521 atatgccctt ggaaaatatt gaaataaaat ggggaacaaa aatgataagg caatggagag
     8581 agattttcat acagcagcac cctaaaccat gattgcctta tacttcttca gcatagtgca
     8641 gggattgtat ttttctttga atcgtctgag gccgggaagc cccatgtcat cctctttatt
     8701 tatatattcc tcatccatcc tcttcgccag ctcgcttaca agatatgtat ggacacctct
     8761 gtgggaattg ttggtaagct caacatggga ttgggccatg tcgccggata ctttctcata
     8821 caggcaaaat gccgccactt catcattttc caggactact ccgcccttca aattgaattg
     8881 ggcgatgctg tccagcatct tatatgtcgg ttccagtttg tcttccgtat tgtggtattt
     8941 taatgtaaat gccttgcatt tatctatgta cagctcaatt ctttcatcaa attcaggcat
     9001 tacagccctt acatagctgg agaggttgtt cctctgattc ctgaacattt tgccttcata
     9061 ctctatgaaa tccgattttc taaatatata gtcaaaagaa tccctgtcct cttccaaaac
     9121 aaacccaaaa ttcttaaaaa ctcctgtgta ttcttgaggt attttagaga aaacagggct
     9181 tgtgcccatt ttattacagt acttgataac tgcttctatc ccctgtgccg tatcccccaa
     9241 aggcatataa aagcaggcag gagccatatc agaccgtatt attactgctc cgttgctttc
     9301 tgctattttc agcttataca agttattata catatataga tatgagaact ggtaatccga
     9361 gcagatatat ccaaacttgc cgaaaatacg cttatcgcca agtgtgattc ccctgaaatt
     9421 catatgcttc tgcctccttt gaaaaatgca gagcctccgc agaaaagggc ttcctaaaag
     9481 gcagccccta ttcctgatat tcatttatcc ttaaccggca ccaggctttt tccgtcctga
     9541 ttcccggctt ctacagttcg atatgtgctg caatgccata tcccttattt cctgccttgg
     9601 gccctacgaa actccatttt cccggaattc caagatcttt ggccaacccc tccatagtat
     9661 acatcattat gcccgcatca atataatttc ttggattctg aggctccaga accgtcagtt
     9721 ccgcttctcc gtccttcaat attattctgc aaggctgcaa atttttataa gacggcgcat
     9781 tccttatata gaagaacagg tctgccatac ctctgctctc caattcctca taggccgccg
     9841 gcctacccca ttcatgcaaa taaacaattt cacccaaggc aagtctcgcc ctgtcggtaa
     9901 tctcggcatc cgcatatttt atgccataag gattctgcct gtattccgaa gcttcatttg
     9961 ttacggatgt gcggggtttt ttattcttta ttgccttctc atcgggtatt ccgaatgcaa
    10021 gaaggtaatt gatccttgat tcactgtctc ccgacagttt tgcctttata tcgccggata
    10081 catccctgat atcaatccag cagctgccca gatacatctc ctgcagtttt ttgactaccg
    10141 actgcatatg atacgcgccg aagaattcat cttcgaaatt ttctttggta acggcaagcc
    10201 ctatataatg cgggctttta atcataacac cgctgtaacc gccgacacct ttcaatgcat
    10261 taaagacatc actgccgttt ttcagcatga taagtgaaaa gcctttttct ttccctgcat
    10321 cggcattgat tttttcaagg tatactctga tatctgaaag caccaggtcc tttaaattct
    10381 cttcttcata atcccttaca gattgaaggt ttgccaggaa atctctaaag ttcataatca
    10441 tgcctccttt actaactctg tttattatat cttaattcat agttcaataa atatcaagta
    10501 atagtgttta ttgtggggac acggggacgt ttcgggacac ggggacgttt cccctgtccc
    10561 acttccagtg ggacacgcga aacgtccccg tgtcccgaaa cgtccccgtg tcccgtccat
    10621 gtcccataag ttaagcgtat agatgtataa taaagtgtgg taaacttaca ttaaggaagg
    10681 agatgataat attgagcaat tgtaaaagta ttaaggaaga aatgctggag aaaaaaatat
    10741 gggctgtgat aggtgcaaac aatgattccg ataaattcgg caatattatt tataagaagt
    10801 taaaatgcga aggctacaag gtatatccgg taaatcccat gtgcgaatat gtggatggga
    10861 accggtgcta tccaaatctg tcatcacttc cggagatgcc cgaggttctg gatatggtgg
    10921 tatctccaga aaaaggcaag gcattcatag aggaagccgc tgcccttggt ataaaaaata
    10981 tctggctgca gccgggaacc tgtaatcggg aaatcctgga gctgctgaag gcaaaacagt
    11041 tgacaggggt acaggcatgc gttctggttg cattatgctg attgcttgtt accttaagaa
    11101 taatcccatc acaaaataat ttagaacaag gacagtagcc agagagacaa aaaacttcca
    11161 gtaatctttg tacaagtctc caatgcttac tttaaggtat tcgcaagtaa atatctgaca
    11221 gagatgcatc ggagagaaaa agtatcccac aaaagcccaa cagaatgtaa aatgacaata
    11281 catcagcagc atgttgtctg ataagggcag tgtaatcagt ataggcaata taacccccaa
    11341 tgcagtggga gaaaaacctg ttgtaatacc aaaaaacatt gaagcggcta ttatcccaag
    11401 tataattgtg tttggattca taagaattga agccaaaaat gagtttagtg actccatccg
    11461 tcccattatc ccctggatga gatatactcc aataagggcg taaagcacat taaaactaaa
    11521 tgcccggaga gaatccatca gaaacgtctt tgtgggatga atcaaatata caatcagcag
    11581 attgacaagc acccctaaat aaaatggtac tcccagcaaa agattcagca gcacagccac
    11641 atatatgggt gaggtgaact ttaagagctt gaccagattt atccattcaa aactataatg
    11701 agggcttgga ctgctttgtg cctttcggat gtatatatag taagctgcta taaaatacat
    11761 catcatgaaa atactgttca atcctataag cttatatatg gaaatccggg gtgaaatcga
    11821 tgccacaact aaaaaactcg tagcataggg cagaatgttc atgggaatat gccgaaaaac
    11881 cagatttatg attgctctat gaggctttga tatgttcgac ctttccccca actggtcaac
    11941 caagggaact gacattatgg ctcctccggg gacagtcaac atgccgatta acgcaggtat
    12001 aagcataaga attgcttttt cgttttttat aacttttgtc aggttatcca ggacctcatc
    12061 aatgatctta tattttttta ggagtacccc gataattgag aactcggcta caactaaaag
    12121 ctgctgaact ttattgaagt taaaaaaggt attggaaatg acattggcaa aatcaaaggc
    12181 tcccaacccc cccaataaag ccataagcac tgcacaggca caaatggcaa tccctaaagg
    12241 gacttttttt ctgcttaaaa ccggaatgat taaaaaggac cctattaccg caaagaattc
    12301 ctgcatatgc ttcaccgtat tttgcttcct ttcttattct catttattgt tgttgtgtca
    12361 gaaacataat tattttcttc cagatttcct gtgtctcaat aaattgttta taatgcctct
    12421 cgacattttt attgtaacgc tccagctctt ttgaaatatt cataaaatcc ttgcgtttaa
    12481 agtaatcaca gattttcgga agcgtatctg acagcttctc gcgcatctcc attattttat
    12541 tttcataatt ctctctttga gtaatataaa gaagtaacgg cttatacctt acccagccta
    12601 tgcacgcccg gtaaaagcga ttgacaatct gttcggaatc agctttaata tacttcaatt
    12661 taattggaag taccccatcc atcaaatgat tatatgtgga aaaaccatca tgaaatgtat
    12721 agcagggcac acgcagcact ttgcgctggc taaggcatgt acttaaaaag gtatcctctc
    12781 cccgggcccc cggcggattg taaaaggagc ttactctgtc aggattagta aggtttatgc
    12841 ataaattcgc acccgatata aacttggtat ggttaacttc ctgcacctcg tatgctttat
    12901 cgctcaccag aattttagta tctgcataag taactccgcc gttattcatt attgaacgga
    12961 tcgattccca attaattata tcattgctta ttgcctcaat aaacaatcga aaatcggttt
    13021 cagtcattgt gtcattaaac tctatgtaag ggataggaga gatatatccg cagtgatgtc
    13081 cattggtaat gtctgcctga cttatatgtt ccaaatgagt tgacaacacc tgttgtccac
    13141 cccatatagc agttgatcgc gtattggtaa cagccatggg gtattcgtca tcgtccagaa
    13201 aaacaagata atcaatacga tttttcattg cggtataaag cacggcgtta cgtttggcag
    13261 catatccact tccgaaaaac agctttgctt cattttcatt aatcacattt ccccgtatca
    13321 aataaccaat ttcctgcttg attgcattac tgcctatgaa ataagcatca tcaatcagct
    13381 ccagtacttc ttcactaaca tttgtataat ctgtagacct ggtgttttta tatttcagat
    13441 catatgcaac caaaaggttc aagctgacat cttctccccc ggtcaggtgg ctttcgctcc
    13501 agttatatac atatgtcttt agaacctttt gaaaacttct tcgccccgta gcaaaaccga
    13561 tccctactct tatgtccttg ttttttttca cttcagcact ctcctttctt cataaaatac
    13621 aatacttaca tttatataat agtataacaa tagtagtagc cttttgcaaa aaacggcgca
    13681 cataaaaata aaggcggtta cagccgcctt tgaggattgg cactattata ttttaatgtt
    13741 cctcaggctt tttagtattc tttttctcac ctatactcag aacaagatta aggaggattc
    13801 caaatatagc cgcacctgcg atacagggaa cctgcatgcc gaagaagggg atattcccgc
    13861 cgaagccgaa ttgtccgcca agtcctatta tcattatggt tgcaatgatg gcaatatttt
    13921 tggagctgaa caggtcaacc tttttctcaa ccataattgc gataccttga gctgcaatgg
    13981 cgccgaacaa gtatacttca agccccccta ttaccgaggt gggaatggaa tatatcgcgt
    14041 ttacaagagg agtgaagcag gcaataatca tagctataac cgatgctccc atcagaaccg
    14101 gtattgaaaa cactcttgtg attgccatgg cgctgatgtt ttccccgtag tttgtccctg
    14161 cagggcctcc gataaaaccg gataccatat cgccgatacc gtctccaata aggttgaggc
    14221 ctaatttatc tgcaatattg tacctcttgt ctgatccctt tttcttagcc aggtcattta
    14281 cataaatatc aagctggtat acgtgtgccg ttgattccgg tatggttgcg atagcaatgg
    14341 gcatgatagc ggcaactgcc attaatgatg cttttgggaa tgtaaatatc ggaagtgcga
    14401 agattccgct gtttgctgtg tcgggtaaaa ccttgaaaag gtctatgcct gttgcaagtt
    14461 tgattataaa tgcagtaaag catccgatta tcgggcctat cagaagggga agctgtccta
    14521 agaatccctt aaggtatact gacagcagaa tagtagatat caaagtaaca agagaaatta
    14581 tccaagccat actgcctgca aatgccaact gggattctgc cactgagatt gtacctgcgg
    14641 tatcgatggc aatagaagcg gcatcactca ttgcaagccc tgaaagggta aggcctataa
    14701 ccattgcaat agggcctgtg attgttgccg gaagaacctt ctcaatagcg tctcttccga
    14761 aacgttttat tataaggccg gctgcaatgg atacgaaacc ggacattatt atgccgaatt
    14821 gtgcaactgc tattttttcg ttaagagtgt atcccgccag tgcatccgaa gccattaaac
    14881 tgcctatggc agctacgtat gaaaagcttg aaccgtaata cagcggaatt tcccttttgg
    14941 taaccagtat aaagcaaatt gttgcaaggc cgctggcaaa tatggttgtt gaaatgtgga
    15001 agcctgtgat gattgcaact gcgactgttg caggaaacat taccacaacc tgctgcaatg
    15061 cataaaggaa aagtttccat atcggtggtt gttcatcagg tagataaccg attatttgtt
    15121 gattttttgc cataataaca tcctccttgt tttttagtat agcctttgct ccataaaaaa
    15181 atcctgtctg cagctttaca gacaagatct tgtttctctt agatattagc tatattccta
    15241 tagcttgtat ataatcttgc cggtatcact gtaccagact taaagacatt tattcttaat
    15301 aagagtatca cacccaacaa agcttttcaa catattttga tattttttta tagttttttt
    15361 acaattttga ttcaggtgtg aatcagcagc attgcagcat aattactatt tatttattcg
    15421 aattaaaata tataattaat ataaaaggca atatttatta aatattaaac cattaggagg
    15481 gaaatataat gtcttacttg ctgaagcccc tgaaaaacgg aaaattgctc ttgaagaaca
    15541 gattaattat gcctcccatg gctactgcca aatgtgagaa ggacggaaaa ccaagcaagg
    15601 ctctttttga ctattatgat gagaagtccc gagggggata tatctccttg ataattgttg
    15661 agcacagcta tatatcacag caaggtaaag ccagcgacag gcagctttct gccgcggatg
    15721 acagcctgat tgacagcttg aaggagctgg ccggtataat tcacaaaaac ggtactaaag
    15781 ccgtaatgca gataaaccat tccggaagtg ctgcgaagta tgaggttaca ggtatggatg
    15841 cggttgctcc ttcagcagta tccaatccgc gtcacaacgg cccgctgccg aaggagctta
    15901 cccttaaaga aataaaagca ataattgaag aattcagagc tgctgcatat cgggtaaaaa
    15961 tggcaggttt tgacggagtg gaaatacatt ccgcccacgg atacttcctg aatcaatttc
    16021 tgtctcctct tgccaacaag agaactgatg aatatggggg aaacatacac ggcagaataa
    16081 agattcactt ggaagtcatt aaggcagtaa gggaagctgt tggtgaggat ttcccgattt
    16141 ttctgcgcct gggcgcctct gattatatgg aaggcggaac ctccatcgaa gacagcaaaa
    16201 tcgcggcaat ggaatttgaa aaatcaggag tggatataat tgatatttcc ggcggctttt
    16261 gcggatatat cggacgtggt actgccgagc aaggttattt ttccgagctt tctgaggcaa
    16321 tcaaaaaggt tgtatctgtt cctgtcattt taaccggcgg agtcacagaa gcccaagctg
    16381 ctgaaaagct gcttgcagac ggaaaggctg atttgattgg agtaggaaga gctattctta
    16441 atgattccga ttgggcaaaa aaagctgttg aatcactgaa gccataaaga aaaatgccag
    16501 gcaccatacg tatctgtcct tctcgatagg cgataatgtc ggtgcctggc tcatttttat
    16561 ttgttgatta acaaatgttt tattttatat cctttttcat taagcttttt ctctatttca
    16621 gaaacatcct ccaccgatga cctaattatt aaatccctga tccctttgcc ggcaaacatt
    16681 gctatatggg ttatattaat atgcaaggaa gagaatactt cggaaatagc tgctatgccg
    16741 ccgggtacat cttttacttc aacggttata cggcttcctt tttctttaaa gcccattaag
    16801 tctatgaaag catcaaatat atcgctttct gttattattc ccaccaattt tccattttcc
    16861 atgaccggaa gggtgctgat tttgttttct ctcattgcca ctgccgcttc ttccaataaa
    16921 cagtcagcat ctatagtgat tacttttttt gtcattgctt ttcttaccgt tatttttgac
    16981 agaaaataat tgacttcata gatactcaaa gatgtggctt ttgttgggga tactgactga
    17041 atatcacctt ttgtaagaat acccaccagt ttcccgttgt ctactacggg aaatcttttg
    17101 aaaccctttt ctctgaaaag tgccaccgcg tctgcaatag tagtgttgga tggtattgta
    17161 cacggattcg gggtcatcct atttctaata tacatactat tctcctttcg cttatactat
    17221 ttttccgctt cattattatt gtgataccct tcaatatcgt tacaccaatg aatatatttc
    17281 tctctgccct ttatattaca actatatcaa atgacaatgc agcaagcaat aaacaatgcg
    17341 gtggcaggcg cttgcatact gataatccat atcgcatctc ccgaatatag ttttcatgta
    17401 ttataacaca aaatcgtgca taatgcacgt tgttgaggca tattcacatg gtcacaggct
    17461 ctgcagaaaa ggtgttttcc tcttcaaaaa acaaaaaaac ccttgtaaaa tcaagggttt
    17521 tcggacaaaa tatggtggag ataagggggt tcgaacccct gacctcttga atgccattca
    17581 agcgcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg tggagataag
    17701 ggggttcgaa cccctgacct cttgaatgcc attcaagcgc gctcccaact gcgccatacc
    17761 cccacttatt tgttttgaca aagtatatcc tatatcattt cagagaaaaa ttcaacacaa
    17821 ataatcaatt cgtttttgta tgaaaaaatt gggtttccgc actcctgatt attcattcaa
    17881 ataataaagc atataaatag cctcggctct tgtgacaatt ccgctcacgg agctgaagct
    17941 gcccgacctc ttgtccttgt tcagcgccat tttttcggct atataggacc atttaaaatt
    18001 ctcatctccg gttgcttgct tcataagctt ttcaatctcg gtataagtca cggtattatt
    18061 cggagtttcc ggtgctggca gtttagtttt ataaacgttg tttatccaag ccgccgcctg
    18121 gccccgggta agcggggaat caggcctgaa gcttccgtca ggatatgcct ccgcaatccc
    18181 tcttctaaga tatgccgcaa tttcatcctt tgcccagttc cctcttaaat catatggatc
    18241 ccgggcattt tcatctataa ataccccgta ggtacaagtt ctattattga tacttcctgg
    18301 gctcagtttc gtactgatgc tgtttccgtt tatcctgctc ggcagataaa tccaggtgcc
    18361 gttgctgtac ttgtaaatgc ctccgtttgc agggccataa tattcaaagc taagctccgc
    18421 tgatttgtcc ggatttacgc tgctgcttga attggcaacg gatatggtat aaaactccga
    18481 tgcctttatc attccttcct ttgaaggcag ctttatattg gcattgcttg ttactgtctt
    18541 gtctattgaa actgctgcaa tgttatagaa tgtacccttt tctatggtaa gcttcattaa
    18601 cctatctccg gaaaatatct ccgcaccgct tataggtatt gtctccgtaa caatctttct
    18661 tttaccgtat tcataattgg agtcccggta ttcagtaata tatccttctt tcaacccttc
    18721 cctgaatccg cctataaatc cttccctgta tttctcatct tcattgaaga gcatatattc
    18781 ctttattata tcctggtcgt tataattctt tcttgtatcg taattggtta aattcatggt
    18841 gcggtcaatc cttgcaaggc tggctcccct aatgactcct atttccctgc cttgctcata
    18901 ccctctttca aaaatcagga ggtttttctc aatgtttgct ttcctgtatg cttcctcata
    18961 ctttgtcctg tatgcggact tgaaggcagc caggaaggca tttccatagc cttcggaatc
    19021 cttcggcaga aggaatatgg cttttatact gctgtctgaa ggcagcttcc tgtcccattg
    19081 gcttactgaa ccgctgtagt aatccagcct tcctctgccg gcacccatga ttcctccgaa
    19141 cagctcccca tccttgactc cctgttccat agcagtcttc ataggttcga attttgccat
    19201 tctatatcca tattcgtagc cctttttata attaaccctg aattccgtta taaagtcgtt
    19261 cttgtaggct tcagactcca ttttcagccc gaataattgg attatttcta ttgttttggg
    19321 gacatggaga ttccatgagt tttcaaggcc ttcccaatag tccagcctgc cccttatttc
    19381 acccattgaa taacctaatt cccggtcata gttaacctgg gatgcagctc ccgactcagg
    19441 attctcatag ccgaaattat atccctccct gaatccgtcc ctgtagcctt ccaaaaatcc
    19501 atcctcatag tcgttgcttt cttcatccag ttcatagtat tcctttattt ccgaattgct
    19561 gggtattgct ttgctgtagc tctttcttct gtcctcgtcc aaatcctccc atgcagcctc
    19621 catgccgtct gtccagccgg cactgtatcc atccttgtat ccctcgtcag tatcctctga
    19681 gcctgcacac acagtcattc cggaaaacag caccaaggca agcaatacag ctgcagcaat
    19741 tgcgatatat ctggtttttt ctttcattcc aatcaccctt ccccgctatt ttctaattac
    19801 cagtattctg cccgcttccg gcgcactgtt tttgggaagt ccgttgtcct tcgcaatact
    19861 tgtgtatcgg gatcccgtac cgtagaactt ttcacttatt cccctaaggg tatctcccgg
    19921 ctgcaccaca taaaactcaa agctgtcctg gttttcggcg ctttcctcat tgtcagtgta
    19981 ttcagcttct atacggtcaa acaacaaaac accgtagtca tccttttcgg ggcccagttc
    20041 tatatatatc ctgtcaagct tcagggggta tacattcaca tcctgtgtcg gagaagctga
    20101 tatatacttc cagccgaccc aattgactcc ttccgacagc ttcaggtcta tcttttcccc
    20161 ttcctggctt tggaacctta accccaccgt aacaggtgaa taaccgtaag aatatgccca
    20221 tattcctatg gatgagggcg gatatttaat atttatattc tgatcgtcca gcaccacata
    20281 agccctttcc ggctgataac cggcagatat ataatactct gcccttacgg aggtccttcc
    20341 gaattttccc tccggtattt tttcaacggc tcccgttacc cctgagccaa ttgacataaa
    20401 gtatactgca tcagactcca aatcggacag caaaagcctc ttttcctcat cccacgatcc
    20461 gctattgcct tcagcggcct ccgcgtatcc cttgaaggct tcaaagggat cagccttacc
    20521 ctgggcagta aattcattgg cataaaaata tccgtccttt tcagtttctt tgccgccgaa
    20581 ggttacggca ctgaaagcaa cttgtccgct cagcgtttcc gcatcctctc tatgtataga
    20641 cagttcctca attaaaaccc tcctggggtt gttcctcagc tgggaaagca gcttctccaa
    20701 atcggaataa cttccgcggt atgcaaggga aactcccgaa agctttgcat caattccctc
    20761 caactccgcg gtttcagtct ccctgaagct tatacccgga atattaagct tgcttctgtc
    20821 tataataccg tttatcatat acataagctc aggctgttca tcaccggaat aatatttacc
    20881 ggcagtcttc ccgtaatctt caagtaaagc ggcatattct tttcttatgg tactctcctt
    20941 tgacagtatt tcccgcattt tcatatactc ttcttcataa ctggcttttt ccgctttcag
    21001 cctttgtatt tcgagttcct gcggagtaat aacatattta tggaacagcc acagcatagt
    21061 aactacagcc aacagcaaaa ggaggctttt ttcctttgtg ctgatattca ggccgcttcc
    21121 gggcttattg tttcccagtt tcagctccac caggcatttc cccctttaat tcgatatcca
    21181 tataaaagct gtaatattcg ttttcgttgg taacccgtga tacaaaaacc ttctccagtc
    21241 cttcatcctc cctcaaatta tgctcgaata gggctatgga ctccttatcc tttgattttc
    21301 cctcaagttt gatactatgc tgattcatct gcattgcatc aaggaaaagc tcggcaggag
    21361 tattgaccct tatagcttca agcaagaatt catttatcgt atccctgctt tcaatgtatt
    21421 tatccagggc ataaagcccg gacaaatcct ccttcagcaa agccgcttcc ttttccatgg
    21481 ccatgatacc ttcaagcctc ggatccttcc tgactgcttc cagttcggta ttcagagcat
    21541 tgatttcttt acccagcctt cttatggata aatgattaaa cgcaccataa gctataatgc
    21601 caatcaaaac caacactgcc aaacctttaa taaatatcac aggtttttca atatcctttt
    21661 ttgccctctt actgttatag ggtgaaaaaa aattcatatc gttcatacca tcatacccca
    21721 ttgtccctga ttaatgctcc tgcgcaattg acatacgcgt ttatatcccc attcggataa
    21781 atcctgcttg tgctgttcag cactaaagcc ggaatattga aatatccgga aaacaagtta
    21841 tcaattcctc tgatcctgga taggccgccg taaagaagaa gtacttccaa tcttttttcc
    21901 ggttccttgg aagcatagta tttgaatacc ttgtctatct tgtccattat accttctatg
    21961 cttgtcttga caatgtttgt atatctgttt tgctcggaat agccttcctg aaccgtactt
    22021 acatcaataa ttccctgttt gcatgccttg atttccccgc cgctcaagcc tgtcaggctc
    22081 gatatatttt tgtccaggct ataccctccg ctgtcaatag ccctgtttaa tttagccttg
    22141 ccctcgctga taattgtcac attggagctg ctatagccca aatctatagc cgcaatggtc
    22201 ttatcctgcg ggtacaggct gccgttgaca ctgccggcat ggttcaggaa tttccatatg
    22261 ctgttggact ggtaatccat tacctccggc ttcaagccca gcttccttac aaaatcataa
    22321 tgcgtttcca ccaattcctt tggtaccgct gccaccagca cattcagctt ttcctttccg
    22381 tcttccgtaa tcctctctat cgccttatgc tgtattacgt atttgctgaa atccataggc
    22441 aggtgttctt ccatctggta atgcagcaat ccttctatat ccttgccggc cagcctcgga
    22501 agaagtattt cccttgtcag tattgaggtg ctttttacgg ttatgtggca ggtgccccct
    22561 gaaatattgt tttttgccaa ttcctccctg acaactgctg ccagcttgaa tccgtcctca
    22621 atatagccgt tatcgtaggt attgggagga gttagaaaag aaaatgcctt ttttacagat
    22681 attccttctc tttcctgctt gcctgcgata atctttgttt cataggagcc caaatcaaca
    22741 gccgttatgc tgctgcccaa aaacgaaaac accttcaatc ggatacctcc ttccattcgt
    22801 gtatctccca gcctacgttg acaggctcca atatgtgcgg cgggtaatcg cagaacattc
    22861 tggggtcatg ggagtatttt ttgttatagc cggtcctgtc aataatccca acggggcgcc
    22921 tggttctttg cgtgatgctt ccccatatgg tgatatcgcc tttccttaca tatttcgatt
    22981 ccccattccg ttcatcatac ctttcaaaac caaatccgcc gtttatcgca aacaatgcag
    23041 aatgtatcgt gacatccttg ggtgcaacat catatttata gttttccttg acatatcttt
    23101 ttgttctgtt gttccatttc catacaaaat cccagcaggg ttcatcccct cctaccgaca
    23161 tcttaggcca gttgaagtga agtatggaga tatccctgtt tgcaacaagg cccagcatat
    23221 ccttcccgtt ggcctccctt atgtaatatt cctttcctcc cggcttatgg gaggaagtga
    23281 aggtagttga ttcatactct atactcccgt attcaggata gtcatagttc ctgccgtcag
    23341 accagcggaa gctgacagga ggctgattgg gtgccggatc cctcctggtg aggtcttcgg
    23401 cggaataatc gtaccaattg gtagggtctc cgtaggttat atatatatcg ttggcggctg
    23461 cgatagtcag aactcctttt attgtgcctg agataaatat attccccgat tcaaggtcaa
    23521 acttgcctgt tcccccgcca gctgccttat cgacatatat aaccttgtta tatatatctg
    23581 aaattttgta ggtttgtatt tcagcagctg atgcatttcc gttccttatc ttgatatatt
    23641 ctccgtccag atatatgcag gtccttccgt aaaagaccat attgtccttc tccgcccaag
    23701 ccttcagctg ggaattggtt gccggaaact ccagtatgtc cgtctttgaa ggatttccgg
    23761 ccaggtatgc atcatcccgg taagaacttc ttatgttctc ggtatgagtc cccgaatagc
    23821 ttacggtatc aaaaaatacc gggtaatata ccgtcttttt cagagtcgtg ttgtatatac
    23881 tcgctccctc tctggtatac agattcttgt tggtatggta aggcccgtgg ctttcatccc
    23941 cggaggtcca ccagatggag cttccgtcat cattactgac ataaacatga tgcacaaact
    24001 gtttttttct tatcttggcc tctatagtcc tctttatgcc cggattgtct ttggtccaac
    24061 cggttgatac tatggttatg aacctatccg tatcactggg ccttgtaaca ctaagctgat
    24121 aataaccgtc catgaattct ataggaaccc cctgcatatc gtcactttcc ttggtgctgt
    24181 aaaattctac gttgtcgttc aggtgccaga ggtatgcgtt gtatcctgct tcagcatatt
    24241 ccatggcttt tttgccggaa tcatccatta tattgaatct ggcttgagaa tccagcatac
    24301 tcaaggagaa aaatccgaat atcgaagata tggcaagaac aataattact attggcaagg
    24361 cggcgccttt gttgttccga aaccatctca taaaattcag ctcctttcta ctctgcctct
    24421 gttctgctct ttgaaataag atacccctgt acttctatgt cattgggctc cccttctatg
    24481 accagcctca ttctgagctt tctcctgtaa tcaggggact cctggctgtc ctcactcaca
    24541 atccttatgg gctcttgttc cgagaaaata tccgtatttg atatattcct caatacaatt
    24601 tccgcagtac cgaaatccga ggtataggta taagggtatt ctgtaccttg cggcttctta
    24661 acatccctct gaaggtttcc gcttacaatc ctgtacctta ctatttccgg tatgtttctg
    24721 ccgtcctcat tatccacgtc ggtatatatg tatatttccg ttgagctgat tctgtggagt
    24781 gcatcctttt tctcctcagc ctttttcgcc cgatttgctt cactgtatat gttcttttgg
    24841 aagaacctta gttcttcggt tacgtcaacg gttctgctga cacagcttaa gctattctgg
    24901 gcaaggaaaa acatgttata gcccagaagg aataccattg caatcaaagg aatggaaata
    24961 accagctcaa ccagtacaaa accctgcctg ttgtatattg aatttttcac acgtccgttc
    25021 ataacccggc ttctccttct aatcttctgt gagaatgcaa taattcctgt cctgtacaag
    25081 cttcatgcca tattcgttta ccagggtatc cgcttctttg ttgtccgctt caaacctgag
    25141 tttattcccc gttatgccgg cggatgccct gagatttatc aggaacttgt tgttcatgcc
    25201 gctgaccgcc tgggttattg ttatgtccgt gcaggttatg ggagtatccc cgtcataaag
    25261 tgaaaactcc aaggtgtcta tgccgtttat ttccgaggta aactccactg tgagggttat
    25321 gctgccgtca gcctccctcc tgaagcttcc atccctgagc ccaaaagcca gcttcagttc
    25381 cttccttgtc actccattct ccatgtagtc aaagcttccg tcccataggt ttccggtgtc
    25441 cttatcaacg aaataatagc tgtatgcacc gctggttata ctgtgcccat aatcccaagc
    25501 cctccatatt ttttttgtca tcaggcttgt cagattggta tcaaggtcat aggccgccgt
    25561 atcaccctcc ggctgatcat atatcggatc cagccttacc cgggagttat ccgtcagagc
    25621 tttcgggtaa ccggtcaaaa ctatataagc aggataatcc acataaaata tctgttcaag
    25681 aaaatcatcc cccgttgcct ttacttcaac ctgcaaatgg tatatccaat cctcattggg
    25741 atatgctccg gacgtacctg tgggcctcgc cgtcatttcc cccttctccc attcctccgg
    25801 acagacatta tacttcgcat tggccagtcc ggcaaataag gcttctccct tttcatcggt
    25861 cctgctccat gaaacaacag tattgctgag gttttttacc gttaccttta catttttggc
    25921 cggcttcgta aaatcctcac cggttattgt ccttaccttc aggggaacat aggagtacaa
    25981 tgtcctctct ccttcaaagg ctataagggt tcctaacact gaaaaggcct tctcttttcc
    26041 ggataacggg tctctcaccc tgacagttac gtcagccttt tttgcggcat tggctatata
    26101 ctccccctgg gaggatactg cactttccca gtaaatgctt gtcatcagcc tgtattctat
    26161 cccatctatg acctttggat cggagcctgt ttcattaagg tagagattct cttttacttt
    26221 ccccgagggg gaataacccg ccgaatccaa tccgagttca ctgttgtaat ccagagtttt
    26281 cagccactcc acctgggtgt aagccagatt tattgccgta agcctgatat tgttccccct
    26341 ggaatgctgt atcgaatagg cggacaaagg cagcagaaaa agggatatca tacccagcag
    26401 cgctatggat actattattt caattattgt gaagccttta tcccgcctca ttctcatgta
    26461 tgctccccct aaccggtaaa gctttgcaga taccggtaaa taataccgtc accaaaaaac
    26521 aatgtgataa aaaaggaaat attgataaag ggtccgaagg gtatctcatc cttccttccc
    26581 tttctgccgg aaatcagcag gtacaaggag accagcgccc ctataataaa ggacagcaat
    26641 atgtttaaaa cagttttttt aaatccgagt ataaggccga gagctgctgt cagttttatg
    26701 tcaccgccgc ccatggcacc cttggataca actgcaataa acagaaaaaa aagtcctccg
    26761 gaaagaaagc cgtaaatgct gtccctgaat aaaagcgggg ttcctaagat aaaatggccc
    26821 agccccttgt ataaaaccgt tgccgccaga atggccagca caagcccatc cggtattatc
    26881 cgttcataat agtcaatcag gcatattact ataagaatgc ttgccataaa acagtaaaaa
    26941 tagaattcga aagataaacc gaaataataa aagataaata aatataagat accgtttaaa
    27001 gcttcaataa tcgggtaccg gggcgaaatg ccctccccgc agtacctgca tttccccttc
    27061 agaaatatat aactaagtat tggcatcagg tcgtaccagg ccaaggaatg gctgcagcgg
    27121 gtacagcggg agggaggaga gactacggat tcccccctgg gtatcctgta tatgcataca
    27181 ttaaggaaag accctattgt tatgcccatt atgaatactg gtatgtacat gcgcagcctc
    27241 ctttttcagg ttatccctgc acccgtttac tagtcaccgg caccttcaac gctgacggtt
    27301 actttaccat cgccatcaat cacaacttta taagttcctg attcagtagc tcctttcggt
    27361 acttccggcc attcctgcaa ataattactc cagcctaatt tcgtgccatc tgtcgtttct
    27421 cctgtatcct tatcccatgt aacggcatcg gtgccgtctg caacggccat agaggcggca
    27481 ctctgcaata tttttttatt agtatcatta accgtgtctg ccgcttcttt tcttgaaact
    27541 gtatactttg gcactgcaac agccgccagt atgcccagta tggctatgac caccaccagc
    27601 tcgactagtg tgaagccctt cctgttgcgg actttcttta agaaccattg taacataata
    27661 ttttccctcc taaaatctta tataatgata taaaattaca tgcttgtccc gttttttcaa
    27721 tcacctcccc caatgataaa tgccgcctta ttaccctgac atatttatca tgtcaaacat
    27781 aggcaaagct atggacataa ctataaaccc gattataagt gccattacca caatcaaaat
    27841 gggctgcatc atttccatca tcttctggat agctacctcc acctcctcat cgtagaaacc
    27901 ggcggttttg gcaagtattc cgtccagatc cccggactct tcccctatct taatcatggc
    27961 gtccatcatg ggaggaaata ccccgatttc ctttatggtt tccgaaaggg acaacccttt
    28021 gcggaccccc tccttgacac tttccatttt ttctcctata tatttgttat ccattaccct
    28081 ggaaaccagc tctattccct gcaccagagg tataccgctg gacagtattg tggacagggt
    28141 cctgctgaat tttgacgcag ctatcttgat attggtgttt tttatcaccg gcagcctgag
    28201 cttccattta tcaaacagct gcctgccgaa ttccgatctg ccaaagtacc ttaaaccggt
    28261 aaacagcccg gaagccgcta atacatacac ataccaccgg cgtatgattg tatggcttac
    28321 agccaataat atccttgttg gcagcggcag ccggataccg ctgctttcaa acatttgtat
    28381 aaagccgggc agcacggcta tcatcatgaa tagcaccact gcggtggcta cacctgcaag
    28441 cactatggga taaatcattg cgccctttac tttattttct atcttgaatt ccttttcaaa
    28501 atgcgcggac attctttcca taatggtgtc aagagttccg cttacttccc ccgcttccac
    28561 catgcttatc agtatggatg gaaataccct ggaattttcc ttcagggctt cagagagggt
    28621 cattcccttc tgaacatttt cccatgcatt gtttatggct tttctcagca aattgttttc
    28681 tgtctgccgc tccaatatgt ccaggcactt tacaacgctg actcctgcgt tgagcatggt
    28741 atggaactgc ctgcagaaaa ccgccatatc cttctttctc acctttgcaa aggtgacttc
    28801 catactgcct ccgcttcccg ccgtcttacc caccgatatg ggcatatagc tgctgctctt
    28861 cagcatggct acaagctcct gctcatcctt ggcatcctgc agcccttcaa ttatctggcc
    28921 gttttcggcc accgccctgt atttgaattg catagctcaa caccccctga atatgatatt
    28981 tacagcctta tcatgtcggg gttcatactc cgggtcatca aggtatccct gctgatcagg
    29041 ttccttctgt acaggttcat catcgaagag tcaagggtct gcatcccgta tttgccgccg
    29101 gtctgaataa ccgtgcttaa ctggtgagtc tttccttccc ttatgaggtt ccttacggct
    29161 tcagtagcca tcattatctc aaaggcacag gcctggccgt ttccatccgc tctgggcagc
    29221 agctgctggg atattgtcgc ctgaataacc gatgcaaact gtacccttat ctgacgctgc
    29281 tggtcggagg gaaaaacatc aatcactctg tcaacagtat ttgcggcatc cacggtatgc
    29341 agggttgaca ggaccagatg ccccgtttct gctgccgtaa gggctatgcc tatggtttcc
    29401 aggtctctca tctcgcctat aagtataaca tcgggatcct ggcgcagagc cgccctcagg
    29461 ccatttgcat agctcatgga atcgcttccc atttcccgct ggtttactat gctttttttg
    29521 tgcctgtgca ggtattcgat gggatcctcc agtgtaagta tatggcagct tttctctctg
    29581 tttatccgat ctatcatgta agccagggtt gtggacttgc cgcttccggt ggggcctgtg
    29641 acaagtatga gcccattccg cagctccgtc aggtcattta ctatcattgg cagccccagt
    29701 tcttccatgg aaggtatatt caggggtatt acccttatag ccatgcagtg gctgcctctc
    29761 tgccggaaaa tattaattct gtatctgcct accccttgga tggaaaagga aatatctatt
    29821 tcgccctttt ccttcagcac ttccttctgc ctgtcattca agacgctcat tgccatcttt
    29881 tcggtttctt ccggggttag tacggagtcg cctgccttta ccagtcctcc gtttatcctg
    29941 tatatcgccg gcattcctac cgttatatgg atatctgaag cttttttccc gacaccgtca
    30001 tttataagtt caataaaatc caattggctc cctcctaatc aattgtatga cctatcctta
    30061 aaacctcttc cattgaggtt atgccttcaa gtgctattct ggctacgctg tcctgcaagg
    30121 tagtcattcc gttctttatg gcctgaacct ttatatcctc cgtactgccg cgcttatcta
    30181 tgagcctcct gatatcctta tccaggtaca taacctcgtg aatgggtact ctttccctgt
    30241 agcccttgat gcatttattg cagccttttg gcttgtacaa tatcaattcc tttcccctat
    30301 ccatctttaa cagttctttt tccttttcat cagcccggta tgcctcttta cagctggggc
    30361 agagcctctt tacaagcctt tgcgatatta tccccactac cgaggtggat accatatagg
    30421 gttcaattcc catatccata agccttgcca ctgtcgatgc cgtatcattg gtgtggagcg
    30481 tggacagtac catatgtccg gttatggatg cccttaccgc aatttccgca gtctccgaat
    30541 ctcttatttc tcccaccatt attatgtcag gatcctgcct gagaattgaa cggagcccgt
    30601 ttgcaaaggt taatcccgcc ttgttgttga cctgtacctg attgatgccg tgaatcttgt
    30661 attcaacggg atcttcaatg gttatgatgt ttctgtcctc tttgttgatg tccttcagca
    30721 cagtatacag tgtcgtactc ttaccgctgc cggtggggcc tgtagcaagt atgattccgt
    30781 aaggatgatg gattattctg ttgaacacct ccaggtcatg gtcggaaaac cccagatcct
    30841 ctttggaaaa catgaaacca tccctgtcaa gaattctgat gacaattttc tcaccatata
    30901 cggtaggcaa agaggatatc ctcaagtcta tgttcctgcc ctccgattcc atttcaaccc
    30961 gtccgtcctg gggaagcctg cgctcggcta tatccatttt ccccattatc tttatccttg
    31021 tgactatagc catatggctt ctcctgccta agctcattat ttcctgaagg tctccgtcta
    31081 tcctgagcct taccctgatt atgtcctcat atggttctat atgtatgtca cttgccttgt
    31141 ttctgacagc ctggcttatt atcgtgttta caagctttac cacaggggca ctgttgacat
    31201 ccgacagttc gttttcatcc agttcttcct gcctgcccgg ctcaaatccc tcttcaaatt
    31261 ccctgataat ctttttgctg ctctcccggg tatagtattt gtttatagtc ctgctaatca
    31321 cggattttgt ggatattgcg atttttggtt tcataccgct gataagcttt atatcatcaa
    31381 tggcaaaaat attaagagga tcgctcattg caaccagcag ctcattatca actatatcca
    31441 caggcatcaa atcgtatttt tttgctattg cctcaggtat cagttcggcg gccttggggt
    31501 tgataatgta atttgcaagg tcaagaacag ggatgcccaa ctgctcctca ataaccccca
    31561 gaatttcttc ctcactggtg tacccaatct ccaccaaaat ttccccgagt ttttttgttg
    31621 tttttgatga tttttgtata tttattgctt cctccaactg ctgcctgctc agtttcccgg
    31681 aatgcagcag catatctcca agcctcaaac tcgttttcat cttaccacct gcccaataaa
    31741 attaaaaacc ggctaatgct atattagccg gttgcgccac tacgtcacta ttgcataagc
    31801 gcccaagtat aacaatagtt catacgtcct aatggatatt cgtactaatt tactatataa
    31861 taataccaat tacctataca ataggtctta atacctatat tcatctaatt ataatatatt
    31921 ctacataatt tggcaaattc cttttatttt tcataaaatt attcctttac tttcataatt
    31981 tttatttttc aacatcaaat tttccagcat tttcaagact tgcaagcctc ttgaaggcaa
    32041 caaaaaatag ttgtatactt acagtataat cctgctgaag ggggaaaacc cgccgtgaaa
    32101 aagcaaaaga ctcttaggcg agaccgtatc aatcgcacac tggagagcat attcgaatat
    32161 ccgctgaccg ttgtggaagc ccccatcggt tatggtaaga caacggccgt gcgcgaattt
    32221 ctcgcttcaa aagtctgctt tgttttatgg ctttcctttt tatcaggcga ggatacgtca
    32281 accttcttct gggacaggtt ttcggaagaa atctgccggc tggacgagga agcgggcatg
    32341 aaactcaaac ttctcggctt tcccgtcgat gcgccccaga cagcgaccgt tctgtccgtt
    32401 ttaaacgaaa tagaattcaa ggaaaacacc gtgctggtaa tagatgattt tcatctggtg
    32461 aaaggccacc agatcggcgc gtttctttat caattagtca agaatcagcc ggacaacctc
    32521 tatatcacga ttgtgacgag ggacacctca aatcttgatc tactggagct tgctgccaaa
    32581 ggcctgtgca atatcgtgac ccagcaggta ctccgcttta ccgacgatga agtacgcgat
    32641 tactgtgtta ttaggggctg caggcgttta cagtattcac agcatactgc tggattttct
    32701 gaaaacaatg cttaaggacg aacagaagcg aaaagctctc tggtgcagga tgggcgaatg
    32761 gtacctttca aaaggcgccc atatacctgc ttgcagctat ctctgccgtg ggggcgatac
    32821 catgcgcatt ctctcccttc tcaatgatat taacaaaatt gcgtacaact ccccttcttt
    32881 tgaaggattt tcggagatgt tcgcggcaat cccgcgggat actctgttta agtatcccta
    32941 tgcctatatg caatatatag acatattgat gataagcggc gattccgccg aagcccggga
    33001 gggattggca cggctgaatg agtttcagga tgtatatgag cacacagagg acataagctc
    33061 cgatttcaaa aaccgtatcc tcggggaaat cagcacaatg aggattttct ccgtattcaa
    33121 cgatgcggaa cagatggtgt cgcaaatgaa cgaggcgctc cgtctgcttg atggggatgt
    33181 ttcatacctt ataaagcggg agagcgaatt taccttcggc tcaccccatt tactttatac
    33241 atattacagg gagccgggca ggctgaggga aacggcggat tacatagtgc gcaaatttcc
    33301 cttcttcgca aagctggcca acggctgcgg gacgggctgt gattatctga cgatggccga
    33361 gtatgccctt gagacgggtg actggcagag cgctgaaaaa tgcctataag aaattagact
    33421 ttccctcggt atggatgaat attcattgaa tatacatttg tttgccttta tatggtaata
    33481 catctcctac tatttcgcat gtattaacaa ctatagccct ttattgagct ccattatcgg
    33541 agctttttag tttggtgagg cagcaaactc aggttggcaa agcagattta ccatcctgat
    33601 tatattacat gccatatttg ctaaagtaat atgaatcttc gcacgcccta atctcacata
    33661 acggcatcgc cgcagtccat gattccttac cattgtcgca aatcttcttt ctactttaaa
    33721 ccgcttattg ctagcttcct caaattcctt agttgcaacc cgtttcatgt ctcttagtac
    33781 ggcatcgtat cttagaggaa cgtctagtct ccttcccttg cttactactt ttccgttttt
    33841 atccttgtat agacattgat ctcttaatgg acactgttgg caaattgctt tatcaaactt
    33901 gaatacttta aagtccgtat cattagtttt gtttctggtt ataacatatt cttctgtaat
    33961 tattccatta gggcaggtta ctgacttcag gtcttctgat attttaaagt ctcgtagtcc
    34021 atagtatttg acttctctgc tggactcttc ataaaaagct atatttgata tgatttcatt
    34081 atcctttaag tatgctcgat tgcctgttgt cccatatacc ttatctgctg tgatttcttc
    34141 cggcttcagc cctaggcttt cttcaacttt ctctatgagt tctgcaagtt tcttctcatc
    34201 accaacatta aaaggggttt gtactgatgc tagtattatc tcgctgtcct catctactat
    34261 gatatgatct ttatagcctc tttttatatt gccaagtgat ttgtgtgcta tctttgcgtc
    34321 agggtctaca acgcttacta tcttatcctt ttttccgttg gtgtattggt ctataatatc
    34381 ccatagtatg ccaaatacct cttgatattt ttcatttgtt tgaagttcat cataggtctt
    34441 aagatatagg tattctgcat atttctgtgc tatttgcaga tattctttta cacttacctt
    34501 atcatctatc ttgtattctt cttcaatctc attctcaaat tgtaccagca gctgttctgc
    34561 aaggagctca ttgaacttcc tcgtttccct tatgaccttc cggaacgcat cacatatgag
    34621 tctccggttt gatggatagt taacatttgc agctacatcg gtggtgtcta ttaaatatcg
    34681 cttggattta actatattct tttcgataca atttctaaca atctcattga agaattcatc
    34741 aaaactttct tctcccattc tatttgttct aaagtgactt attgttgtat cgtcagggac
    34801 ttcatcgtct aaagataaac caagaaacca acgaaatgca atatccgtct ggattctctt
    34861 tactaccttt ttatctgata gagtgtatag gaattccaac agacagatct taagcatcat
    34921 taccggatct tcagattttc tacctatatc gctatatttt tcttttacta tgtcgtaaac
    34981 aaatgagaaa tctatgattg aatttatcaa aacaagcaaa tgatcttcag gtattagtcg
    35041 atcaaatatc atatgagtaa atacatcaat ttgtggatta ttttttccta acacaatgcc
    35101 taacctccgt attttcaatg ctttacatgc ttttattata ccataatcag cacaagaaat
    35161 atagcatttt atgggaggtt aaaagggttt aagcgtcccc aaaccctttt tgttgtataa
    35221 ttattttttg tttggtatat ttattcaagg gaattagttt ttcagcgctt tcc
//
LOCUS       DAOQUR010000022        34455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1449_length_34455_cov_9.837587, whole genome shotgun sequence.
ACCESSION   DAOQUR010000022 DAOQUR010000000
VERSION     DAOQUR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 34455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34455
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_34455_cov_9.837587"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2679)
                     /locus_tag="PKI50_06410"
     CDS             complement(<1..2679)
                     /locus_tag="PKI50_06410"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HNT03378.1"
                     /translation="MNLNWYNKGIQAISEELSTDLQKGLSREEAAGRIEKYGYNELKE
                     KEKEPVWVKIGRQLKDFLVVILIIASIVSGMVGEISDSIVIIAIVIVNAVLGVVQEGK
                     AEKAMEALKKMSAPNAKVLRDERIETVPAKNLVPGDVVFIEAGDSIPADMRLIESSNL
                     KVEEASLTGESVPSEKDASKAFDTEVSIGDRLNMAYMSTIVTYGRGKGIVVNTGTNTE
                     IGRIAEAIQTFEDETTPLQQKLNELGRWLGLACLIICAVVFAIGFMRGGDILEMFLVS
                     VSLAVAAIPEGLPAVVTIVLALGMKRMVRRNSIVRKLLAVETLGCVTVICSDKTGTLT
                     QNEMTVVRAFTDGNIYKISGQGYNPSGEITFEDEKINPETEPGLKLLLSAGVLCNDST
                     LEQKDARENLWGIIGDPTEGALVVAGAKAGFDKAKMNEAHARIEEIPFDSERKMMTTF
                     HDNFIQNKIVSFTKGAPDIILGRCSKMLQNGSVIDMTEAMKQQIAKVNSEFAGDALRV
                     LAFTYRKYDKLPEAISPDSIENNLIFIGLMGMIDPARVEAFEAIKTCKEAGIKPVMIT
                     GDHKDTAVAIAAELGLMEEHSGVLTGNELDKFSDEELFNRVENTSVYARVSPEHKVRI
                     VEMLKKRGHIAAMTGDGVNDAMALKKADIGVSMGITGTDVAKGTADIILMDDNFATIV
                     SAVEEGRIIYSNIRKFVFFLLSCNIAEIFIIFLSILFNMPVPLVPIQLLWLNLVSDSF
                     PALALGTEAGEPGIMKKKPRDTKEPILNKSLSTGILILSIAQTAAVLAAFRWALNNYD
                     KDLETARTIAFTTLVVVELFMAFTCRSERYPVIKLGIFSNRALVLAAITSLVLGLAVL
                     YIPFLQPIFKTVSLGIRDWVMILLLSIIPVLTGEIYK"
     gene            complement(2839..4437)
                     /locus_tag="PKI50_06415"
     CDS             complement(2839..4437)
                     /locus_tag="PKI50_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNT03379.1"
                     /translation="MPAAQQELQKEIRRRRTFAIISHPDAGKTTLTEKLLLYGGAIRL
                     AGSVKARKAQKYAVSDWMEIEKQRGISVTSSVMQFEYNGYCINILDTPGHQDFSEDTY
                     RTLMAADSAVMIIDSAKGVEEQTRKLFHVCKMRGIPIFTFINKMDRAGKDPFELAEEI
                     EKVLGIQSFPMNWPIRVGGSFLGIYDRKLSQIELFNGGDHGQSIVGSSKGNVDDPVFS
                     DLLGEDVHNKLIDDIMLLDVAGDEFSRRKVLKGELTPVFFGSAMTNFGVQVFLEEFLE
                     LTTPPLSRNADIGEIDAEKEEFSGFIFKIQANMNPTHRDRIAFLRICSGKFTKGMEVN
                     HVQGGKMIRLSQPQQFLAQDREIVEEAYAGDIIGVFDPGIFKIGDTLCEGGSFRFEGI
                     PVFAPEHFARVYTKNSLKRKQFMKGIEQISEEGAIQLFRQKDAITQEFIIGVVGILQL
                     EVLEYRLKNEYGVDISMEHLPYKYIRWVKNPEFNPETFRITTDTMIVQDQSLNPVLLF
                     QNEWSIRTVTERNNGVELVEILGR"
     gene            complement(4561..5355)
                     /locus_tag="PKI50_06420"
     CDS             complement(4561..5355)
                     /locus_tag="PKI50_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNT03380.1"
                     /translation="MDESYVFFNGKIVPEKEVSISIRSKVVNYGLACFEGIRAYWDEE
                     GQQLYGFRTTVYIRPLAYKGGEGLNPTLLDDDNRLLIYCQPLGNFSGKQEMHVAFTSW
                     QRLRDNMLPPRTKASAAYLNSALASLEVLRNGFDEALFLTDNGNVCEGPGENIFMVKK
                     GKLVTPPPSDNILEGITRETIITLAKEEFGMEVVERSIPRNELFSAQEVFFSGTAMEV
                     MPIVEVDRRTIGAGRPGDICTKLKSLFFEITVGKNPKYAGFCTPVY"
     gene            complement(5377..7056)
                     /gene="ilvB"
                     /locus_tag="PKI50_06425"
     CDS             complement(5377..7056)
                     /gene="ilvB"
                     /locus_tag="PKI50_06425"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNT03381.1"
                     /translation="MRLNCAEALLECLREQGVDTIFGYPGGTVINIYDALYERKDITH
                     ILTAHEQGAAHAADGYARATGKVGVVLATSGPGAANTVTGIATASRDSVPMVVITGQV
                     ARSLLGKDSFQELNITSITKTITKKNYLVMNPETLPKIVREAFEIARSGRPGPVLIDI
                     PKDVQQAVIEYEPAEADTGYDTSNLPEMSCETDLYLDKAVELINNSKRPLIYAGGGVK
                     ISDSCRELLEFAEKIKAPVACSLMGTGVFPGTHPYFTGLAGMHGSRCSNIAICNCDLL
                     IAVGARFSDRTICKADSFAPYANIIHIDIDPEELGKNVDTKIPLCGDVKKYLRLLLER
                     IDEKKEGIWNKQIQSWKEAYIVSYKSKGSLNPQYLMKKLYELTQGKSIITTEVGQNQM
                     WTAQYYPFADPRTFISSGGLGTMGYGLGAAIGAAVGKPDCKVINIAGDGSFRMNLSEL
                     STITKYKLPIVQVVLNNSSLGMVRQWQKIFHGERYSHTSLADEVSFTKLADAFGIKSA
                     KITENDEVEKAFKEAFDMNEPVVIECIIHPDDLVLPMVPAGEPINECIDDF"
     gene            complement(7072..8142)
                     /gene="leuB"
                     /locus_tag="PKI50_06430"
     CDS             complement(7072..8142)
                     /gene="leuB"
                     /locus_tag="PKI50_06430"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNT03382.1"
                     /translation="MKIALIKGDGIGPEVTEQALKVLYAVSERFKCKFEYREALLGGR
                     AIDETGVPLPDETVDICAGSDAVFLGAVGGPKWDGLPGNLRPEAGLLGIRKALKLFAN
                     LRPVMLFPQLRTASPLKAEILSQGFDIMIVRELTGGAYFGEKKRVETKDGIKAWDMME
                     YTSREIERIAGIAFGIARKRRKRITIVDKANVLESSRLWREVTGSIMKDYGDVETAYM
                     YVDNAAMQLIRNPGQFDVILTENLFGDILSDEASMLSGSLGMLPSASLGASKACLFEP
                     IHGSAPDIAGKDIANPLAAILSGGMLLKYGFGMEKESDAVLEAVNNALDKGYRTADIM
                     QPGMTLVGTEKMGSIVAENILI"
     gene            complement(8139..8633)
                     /gene="leuD"
                     /locus_tag="PKI50_06435"
     CDS             complement(8139..8633)
                     /gene="leuD"
                     /locus_tag="PKI50_06435"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNT03383.1"
                     /translation="MIKGRAFKYGDNVDTDVIIPARYLNITDKAELASHCMEDIDCDF
                     VKAVKPGDIIAAGKNFGCGSSREHAPLVIKLSGIKCIIAESFARIFFRNAINIGLPIL
                     ECAAAAKDMEKGDLVEADIKTGRITNITKGRTYQAESFPEFMREIMKAEGLINYVRKR
                     GKGL"
     gene            complement(8630..9895)
                     /gene="leuC"
                     /locus_tag="PKI50_06440"
     CDS             complement(8630..9895)
                     /gene="leuC"
                     /locus_tag="PKI50_06440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT03384.1"
                     /translation="MGMTMTQKILAAHAGIEEVHPGQMIKARLDLVLGNDITAPVAIE
                     EFNRIGANRVFDKSKVAIVPDHFTPNKDIKSAENCKLVREFSRKMEIENYFEIGQMGI
                     EHALIPEKGLAVPGDVIIGADSHTCTYGALGAFSTGIGSTDMAVGMATGEAWFKVPGA
                     IKFILKGKPGKWVSGKDIILHIIGMIGVDGALYQSMEYSGDGVGHLSMDDRFTIANMA
                     IEAGAKNGIFEADGKTLEYVSEHSERIPKIFKAGADAEYNRVIEIDLGAIKPTVAFPH
                     LPENARSIDEVGDIRIDQVVIGSCTNGRLEDLRTAAKVLKGSKVSSSVRTIIIPATQK
                     IYLEAMKEGLLEIFIEAGAAVSTPTCGPCLGGHMGILAKGERAVATTNRNFVGRMGHP
                     ESEIYLASPAVAAASAITGKISSPEEVLG"
     gene            complement(9846..11498)
                     /gene="cimA"
                     /locus_tag="PKI50_06445"
     CDS             complement(9846..11498)
                     /gene="cimA"
                     /locus_tag="PKI50_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNT03385.1"
                     /translation="MSVGNWNYCKKIYIYDSTLRDGAQGEGISFTLRDKVNLTMRLDD
                     FGTDYIEAGNPGSNPKDMEYFDRIRKVKLKNSKLVAFGSTRRANTKVHEDDNILSLLR
                     AETPAIAVFGKSWDFHVNSIIRTTLDENLRMISETIGFMKEKGKEVIFDAEHFFDGYK
                     SNPDYALHTLKAAQDAGADWLVLCDTNGGSFPWEIGDITGKISNILETPIGIHTHNDC
                     GMAVANSVMAVQNGASQVQGTINGYGERCGNANLCTIIPNLQLKRKYCLIDDCKIKSL
                     TQLSRFVSEVANISHNEYGPYVGNNAFAHKGGMHIDAVLKHPVSYEHIAPELVGNERR
                     VLMSEVSGRSTILKKIQKYAPGLSKDSSEVKEITDKLKKLEHEGYQFEGAESSFELLI
                     KRSLGKDNEFFKVINFRVWCDDKGDESNSAGAVIKVEVMGVEEISAAEGNGPVNAIDK
                     ALRRALELFYPQLKGVRLTDYKVRVLDTAQATAAKVRVHIESTDGEFVWGTVGVSTNI
                     IEASCFALVDSIEYFLGEWEKREAKKYGNDNDAKNSGSSCRN"
     gene            complement(11566..13080)
                     /locus_tag="PKI50_06450"
                     /pseudo
     CDS             complement(11566..13080)
                     /locus_tag="PKI50_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            complement(13103..14104)
                     /gene="ilvC"
                     /locus_tag="PKI50_06455"
     CDS             complement(13103..14104)
                     /gene="ilvC"
                     /locus_tag="PKI50_06455"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNT03386.1"
                     /translation="MSNVKMYYEKDANLEVLKGKKVSVIGFGSQGHAHALNLRDSGVD
                     VCVGLYKGSKSWESAESHGIEVYEVAEAVKKGDFIIMVTPDEKSAEIYKESVEPNLRE
                     GKSLMFAHGFNIHYGQIVPPENIDVFMVAPKSPGHLLRSQYQEGKGVPCLIAVHQNYS
                     GNAKVNALAYAKGIGGTRAGVLETTFKDETETDLFGEQAVLCGGVTELMKAGFETLVE
                     AGYAPENAYFECLHEMKLIVDMIIQGGFGYMRHSISDTAEYGDYVSGKRIITEDTRKE
                     MRKILSEVQDGTFAKNWLLENQVNRPSFNAMRKKEQSHQLEQVGKELRKMMSWINKK"
     misc_binding    complement(14159..14358)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14792..15724
                     /locus_tag="PKI50_06460"
     CDS             14792..15724
                     /locus_tag="PKI50_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNT03387.1"
                     /translation="MNNFTDYLDINREVLDGLRYYKPVVALESTIISHGMPYPDNIET
                     ALSLEKIIRENGAIPATMGIIKGRIKIGLDREEMEFLAKAKDIIKTSRRDIPFVVGMG
                     LSGGTTVAATMILSQYAGIRVFATGGIGGVHRNAEKSFDISADLTELSNTNVAVICAG
                     IKAILDIGLTLERLETFGVPVIGYRTDDFPAFYTRSSGFKIPMRAGSPRECAKIMKTK
                     WDLNLDGGIVVANPIPKQYEAQKDNIDKAIELALFEADKNGITGKAVTPYLLGKVKEI
                     TAGESLAANIALVRNNALLAAQIAVEYSKLENGS"
     gene            complement(15967..17019)
                     /gene="mtnA"
                     /locus_tag="PKI50_06465"
     CDS             complement(15967..17019)
                     /gene="mtnA"
                     /locus_tag="PKI50_06465"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNT03388.1"
                     /translation="MKKIFEAMRSVKWENNELIILDQTKLPDTTEFIKLDKAEDVWDA
                     ILHLKVRGAPAIGIAAAYGLCVAMRDTGAKSHEEFMTELKKNSQYLASSRPTAVNLFW
                     ALRRMERAAFDNKNKTVEELKEILIREANAIRDEDESMCRAIGENALTLFHDGMTVLT
                     HCNAGGLATAMYGTALAPIYLAKEKGWDIKVFADETRPLLQGARLTAWELMQAGVDVT
                     LICDNMAAAVMAKGLIDAVIVGCDRVAANGDTANKIGTYGLAVLAKAHNIPFYTAGPT
                     STIDIETPSGNEIPIEERKPDEITCGLGRRTAPLGVKTYNPAFDVTPHELITAIITEK
                     GVVRQPYDEGIRKLFE"
     gene            complement(17016..17867)
                     /locus_tag="PKI50_06470"
     CDS             complement(17016..17867)
                     /locus_tag="PKI50_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNT03389.1"
                     /translation="MAVLDCREAARSIKEELAKKVQLLAAEGLKPKLGIIRVGARPED
                     ISYEKGIIKNCESIGISCEVTALSDDISNEDFMKALEAANSDRSINGILVFRPLPAHI
                     DEDRARNMINPAKDIDCMNPYNLAKVFEGDDSGFEPCTPAAVMEIIRFFGINLQGENV
                     AVIGRSMVVGKPLSMMLLKENATVTICHSRTKDISSIVSRADIVVAALGKARAVGADY
                     IKPGAVVIDVGVNGAPEGGICGDVDFEKVKDIASKITPVPGGVGAVTTLMLLKHVVEA
                     AQQGSAL"
     gene            complement(17898..20096)
                     /locus_tag="PKI50_06475"
     CDS             complement(17898..20096)
                     /locus_tag="PKI50_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT03390.1"
                     /translation="MNKSKNRFRKLIFLIVIVLFIGGSLIPAYCREEPGKMPSFSGGA
                     GTPEDPYLISTAEDLLAFSEVGWNAGYMEASYKLTGNIDMGENTWNPVGVNGLSFSGT
                     FDGNGKTITLRNIADSRNMGLFGGTDRRSSIKNLTVSGQISKAISSEEEACFGLIAAW
                     AEGSIENCITEGSVDLSVNVPGNFYFGGAAGHLLGRIDSMINKADLTIKISGGDRLYM
                     GGITGDAMGKEAGIFNLTNQGNITATFDGIGAVGGLAGFCGFGAEAENLLNQGNVTVM
                     QTRAPNSSRSAAGGIVGELRDSGMDKALNKGSIYFEHSGPYANEEIMAGGIAGESEIA
                     ELKNVGNEGNVETKIARIQLAIGITKPGREVTIENAYSRGRIYASTTDSEGELYVMGI
                     GEEVPTNNFYFSGTVRLKAGKKNEEDGDAFANIRLGAKTGIYNYCYWDSKFSPFPAYP
                     FFNKAAVTSKAVNTATGKLSSPVSIGGRQYDNISAALNAWVDMQKGGYLKWTKGAEPS
                     FEWTFGYQIPDFMQYKNGREGKWLNTSDWACEWMDKADRLSIIPDILLNQDMARGITR
                     KEFSALAVKLYENLKGSKIAVDLASPFTDTSDSDVIKAYSLGIVSGIGNGLFAPDASL
                     TREQACTMLTRVYKAVYWEGWALGEDAAYDRHVLDTAGVAKFSDDELISGYAKDSVYF
                     MVKNNIITGIGNNMFGPAPKEGQEGSYGRASREQAFKIAVAMIECFNEFR"
     gene            20270..21364
                     /locus_tag="PKI50_06480"
     CDS             20270..21364
                     /locus_tag="PKI50_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT03391.1"
                     /translation="MISEFIEMAKVSSLSLKEGRFASLLAEKLRGLDFEVYIDKAGEL
                     AGGDTGNVLGRLKGTLDTQPILLCAHMDTVVPGENINPIIRDGIVYSDGTSILGGDDK
                     AGIAAILEAVRYIKENNIPHGDIEVAFTICEENGMFGAKHLDYSWLRSEFAFVLDSSG
                     AVGSVIVQAPAQIKTLSVIKGKAAHAGLAPEKGISAIQAAARAVNNMKLLRIDEETTA
                     NIGRINGGGATNIVCDKVEMEMEARSLTRSKVEAQAKHMLECIDDACREFGAEHETTQ
                     QLSYTELNIPKDAEVIKLVDSAIKRIGLETRLESTGGGSDTNIMSGKGIQAVTLGIGM
                     TNAHTTSEYIAIRDMEKTAELVASIIQEAR"
     gene            complement(21361..21750)
                     /gene="mutT"
                     /locus_tag="PKI50_06485"
     CDS             complement(21361..21750)
                     /gene="mutT"
                     /locus_tag="PKI50_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNT03392.1"
                     /translation="MYIVTAAAIIRDGKVLIAQRQQGSHMEYKWEFPGGKLEPDETPE
                     ECIIREIKEEMDIDIEVDDIYKVVKFKYEEKDILLLCYLCRIIKGDGKAIECNDFKWV
                     TKDELPGFDFVPADLPIVEKLINDKRL"
     gene            complement(21752..23305)
                     /gene="pckA"
                     /locus_tag="PKI50_06490"
     CDS             complement(21752..23305)
                     /gene="pckA"
                     /locus_tag="PKI50_06490"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNT03393.1"
                     /translation="MNLDNLGAKVHRNLTVGKLVEIALKKEGAALTDTGALSINTGKY
                     TGRSPGDRFIVDDPGIHDKVDWGKVNVPISGEKFENLYNKINEYIKDKELFVFDGFVG
                     ADKNYRMPIRIINEYASQNLFAQNLFIKPKEGELEGFEPEYTVIAAPGCHADAETDGV
                     NSEAFIILNFAKKIVIIGGSMYCGEIKKSIFTIMNFILPMKGVMPMHCSANIGEDGDV
                     ALFFGLSGTGKTTLSADKGRRLIGDDEHGWTEDGVFNFEGGCYAKCIRLSKENEPQIW
                     NAIRFGAVTENLVLDPETGVPDYDSEKFTENTRAGYPVTYIDNAVEEGVGGNPSTVIF
                     LTADATGVLPPIARLTKEQAMYHFMSGYTSKLAGTERGIIEPTATFSTCFGAPFMPMP
                     PQVYAELLGKKIDKNNARVFLVNTGWSGGSYGVGKRMKLSYTRAMVNAAISGELDNVG
                     YDTDPIFGLAVPAECPGVPAEVLKPVNVWQDKAEYEKTALKLAADFNANFRKFRGVSE
                     DIVNAGPKV"
     gene            complement(23459..24982)
                     /locus_tag="PKI50_06495"
     CDS             complement(23459..24982)
                     /locus_tag="PKI50_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNT03394.1"
                     /translation="MAKRELKSKSLMIQGTASSVGKSLMCTAICRILARDGYNVNPFK
                     SQNMSLNSYITIEGHEMGRAQVMQAEACGKLPEACMNPILLKPVSDRKSQVIIEGGVY
                     ADMDAVEYFNFKPRLKERISDIYRRLEDRSDIVVIEGAGSPAEINLNHEDFVNMGMAK
                     IAKAPVLLVGDIDRGGVFASLAGTMLLLSPEEKKLVKGVIINKFRGSQEILKPGIKML
                     EEIINVPVLGVIPYFSMNLEDEDSVSEWLKSGPEKGGELDIAVIRLPYMSNHTDFNAL
                     RLHEDVSLRFAEIDKELGEPDVIILPGTKSTIHDMLALKNTGMDKKILKCHSRGSVVF
                     GICGGYQMLGKEISDKFKAESDNERSEGLGLFNAVTEFRKNKFTTLSKGRDNIFGAEV
                     RGYEIHMGETAGTDAENSFITISERGGKGVFGRDGMINKDLTVFGTYIHGIFDSSEFT
                     RSFLNLIRRRKGLVPLENLAPDYWVYKEGQYDKLAETVRSNLDMDKFYEILKTGIED"
     assembly_gap    25028..25127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25203..25814)
                     /gene="cobC"
                     /locus_tag="PKI50_06500"
     CDS             complement(25203..25814)
                     /gene="cobC"
                     /locus_tag="PKI50_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HNT03395.1"
                     /translation="MKGSKTEIYLIRHGETDMNIKGVYYGWTDVGLSEKGIMQAEDLA
                     DILHDVQFDAVISSSLIRAVETASIVSGYTPDMIIKDDRLRELNFGDWEGKHHRELKV
                     RYKEALEKWGSDWRKSAPPGGESFFEIYIRVKSSIEDILKEYRGKKVLIVSHQGTMRI
                     IPLVLLGLTEDAYWSFSAEQGRYSLYEINEEGRCIIRRINSGR"
     gene            complement(25811..26563)
                     /gene="cobS"
                     /locus_tag="PKI50_06505"
     CDS             complement(25811..26563)
                     /gene="cobS"
                     /locus_tag="PKI50_06505"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019279106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNT03396.1"
                     /translation="MRIFLITLQFLTRISVNKNLKATEEDFAKGVAFFPIVGLIIGAF
                     NLTILIIASNILGGVFPVICCLIAGTAITGGLHADGLADTCDGIFSSRDRDRMLEIMR
                     DSRIGTNGTIAIIFDYLLRMSLLAMLTGKALYGAVLLSPVAAKTLMVLLMKASAYARS
                     GPGLGGLYLEKQTMGATVLAAAIGISIILGFGHIWGLVVFGICTVICFLYRSFIYSKL
                     QGMTGDTLGAANEILEVSFILITAVMAGRGII"
     gene            complement(26579..27139)
                     /gene="cobU"
                     /locus_tag="PKI50_06510"
     CDS             complement(26579..27139)
                     /gene="cobU"
                     /locus_tag="PKI50_06510"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNT03397.1"
                     /translation="MGKSILITGGARSGKSSYAEKLAKELGGNVLYIATSIPFDEEMK
                     LRVKRHKESRPREWDTFEGCKGLGRVISEKGSQYKGILLDCITVMVTNLFLEYTGMEG
                     NDCSNEDFEKAEKEIEREIAELMAGIGNSEASVIIVTNELGSGIVPENPLARAFRDIA
                     GRMNQYIAERCDEVYITVCGIPLKLK"
     gene            complement(27304..28374)
                     /gene="cobT"
                     /locus_tag="PKI50_06515"
     CDS             complement(27304..28374)
                     /gene="cobT"
                     /locus_tag="PKI50_06515"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNT03398.1"
                     /translation="MEIFERTAAGIRPSYEESKKEAAERLDKLVKPIGSLGKLEDIAV
                     KISGITGKQKNSFEKKVTIIMASDNGVAEEGVSAAPQAVTALMTINFLKGITGVCVLS
                     KHAGADIRVVDIGVGSDLEYPGLINRKVRKGTSNMAKGPAMSKEEAISAIETGIEMVS
                     QLVKEGYNLFGTGEMGIGNTSTASAVAMAFLGCSAEEAVGKGVGLTEEGYASKKSVIE
                     RAISINKPDPYDPIDVLSKVGGFDIAGMTGCFLGAAYCRVPIVIDGFISAAAALAAYR
                     INPLVRDYIIPSHLSAEPGYSLVINEIGLEPLLDLNMRLGEGSGCPLAFNIIGAAEAI
                     ISDMATFEEATIKDDFLIDIRK"
     regulatory      complement(28479..28653)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            28948..29247
                     /locus_tag="PKI50_06520"
     CDS             28948..29247
                     /locus_tag="PKI50_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03399.1"
                     /translation="MGKEHRRDKEFRIKHFPDNIRDLLCLLADNEDNRATIILNTCDD
                     CVQENVEVVAVIGNLAVFRCHDKFKFVDIHCICEVIVDCETILSELLESKSECKC"
     gene            complement(29295..29747)
                     /locus_tag="PKI50_06525"
     CDS             complement(29295..29747)
                     /locus_tag="PKI50_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNT03400.1"
                     /translation="MLQFIYQVVKKVTNSRKIEDLHPVVQEICRKHIEACKRQGVQII
                     VTNTLRDSEYQEYLYSQGRTRPGNIVTNMRLIGPHGFGLAYDVVPIVKGKAVWNNNRY
                     WRIIGIEGKKLGLTWGGDWKSIVDKPHFEYTGGLKAADLRAGKRPSWW"
     gene            complement(29830..31239)
                     /locus_tag="PKI50_06530"
     CDS             complement(29830..31239)
                     /locus_tag="PKI50_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT03401.1"
                     /translation="MKEKNYVSPILDRIRKQTFEEDMVLAHDLALRYVKQVNDMRVFP
                     DKKAVGNLSVFEEALSEEPEDTKNILNMLGKYGSPATVAQTGGRYFGFVCGGILPSAL
                     CSKWLSDAWDQNSALFVLSPIASKLEEVCESWLKQLLKLPDDTAAGFVSGSSTATICG
                     LLAGRNYLLGNLGYDVTKEGLFDAPQIKVVLGEEAHSTVFKALSIIGLGNARLIKVPT
                     DGQGRIKADEIPEPDNRTLLILQAGNVNTGAFDDFDSVCRKANAAGAWVHIDGAFGLW
                     AAACEKMNRLTKGMELADSWSVDAHKTLNAPYDNGIVLCRHKDALVNAMHMTGSYIIY
                     NENRDGMLYTPEMSRRARAIDLWATLKGLGRKGAAELVEELHCKAVYFSRLLEEGGLQ
                     ILNDVVYNQVLVYYDNDRKTEALIKGVQESGVCWLGGSKWSGKSVMRISVCSYKTTYD
                     DIEKSAEDILRVAALVSDK"
     gene            complement(31297..31713)
                     /locus_tag="PKI50_06535"
     CDS             complement(31297..31713)
                     /locus_tag="PKI50_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNT03402.1"
                     /translation="MIKRHKSVCYCINLRRAANAVTDLYNRVLLPIGLTVSQYSLLIN
                     IGRLKACSVSDLAGYVGLERTTLVRSLKPLFKLKYIEDVSEKGRRSRLLKVTESGQQV
                     LNQGEVLWRTAQAELEKKVGKEKLEQLSEILSQLVE"
     gene            complement(31924..32556)
                     /locus_tag="PKI50_06540"
     CDS             complement(31924..32556)
                     /locus_tag="PKI50_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNT03403.1"
                     /translation="MSAESTIKGYLIVHLEHILDEIGEDETIKILLGFSCPLNSDVEK
                     FLRHKAIEFAKQSLSQTFLVFTSYKDEPTLVGYFTLANKTILVDKAVLRGNVKRKIGR
                     FGTYNPVIKKYVFSAPLIAQIGKNYKYNEQNLISGDELLKMACDKIAEAQFVLGGKVV
                     YLECEDNEKLKNFYSSNGFVEFGKRKLDRDEKDDLSGEYLVQMLKYTKKC"
     gene            complement(33233..33943)
                     /locus_tag="PKI50_06545"
     CDS             complement(33233..33943)
                     /locus_tag="PKI50_06545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNT03404.1"
                     /translation="MANRIVISNIEIEIQKKKIKNMHLSVLPPDGKVRISAPVNTKDE
                     AIKLFAITKIGWIKRQIDKFENQQRQTKREYVSGESHYVWGRRYRMEIGHDYRNNIEI
                     KGNKLILTVRESSTTQQREKVMTEWYRELLKKKLPELVRKWEGIIGIQAESFRIKNML
                     TRWGTCNIKDKRIWINLQLAKKPIECLEYVVVHELVHLLEKSHNSVFVEYMDKYLPDW
                     RVKKDLLNSFILDRYLEE"
     gene            complement(33933..>34455)
                     /locus_tag="PKI50_06550"
     CDS             complement(33933..>34455)
                     /locus_tag="PKI50_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HNT03405.1"
                     /translation="LIELIIERGVDFVDEMPEGIRKDKEAAAEVIENNVRRKIIEKRS
                     TNPLYYEKMSELLMKIIEERKNQKADYKKYLEMIVELTRKVEKPEEYSGYPDRIKKSS
                     ALRAFYDNVTKDEELILELHEKIISIKPDKWLGDKAKEKVIQRGIYEVTNDDDAVDKI
                     FNIVKEQREYYGK"
BASE COUNT         8644 a         8978 c         6269 g        10464 t
ORIGIN      
        1 cttgtatatt tcgccggtca gtaccggaat aatactgagg agcagtatca taacccaatc
       61 cctgattccc aatgaaacag tcttgaatat gggctgcaga aaaggaatat acaacactgc
      121 caaaccaagc acaagagatg ttatagcagc caataccagt gccctgttgc tgaaaatccc
      181 aagctttatg accggatacc gctcggacct gcaggtgaaa gccataaaca gctcaacaac
      241 cacaagagtt gtaaatgcta tagttcgcgc agtttccaaa tccttatcat aattgttcaa
      301 agcccaccgg aaggctgcaa gcacagccgc tgtctgagct atactgagta tcaatatccc
      361 tgttgaaagg cttttattca gaatcggctc ctttgtatcc ctcggttttt tcttcattat
      421 tccgggttct cccgcttcgg tgcccagggc aagagccgga aagctgtctg atacaaggtt
      481 aagccataaa agctgtatag gaaccagagg cacgggcata ttaaacaata tgcttaagaa
      541 aataataaat atttcggcaa tattgcagga cagcaggaaa aagacaaact tccttatatt
      601 gctgtatatg atcctgcctt cttcaactgc cgatactatc gtagcaaaat tgtcatccat
      661 aagtattata tctgcggtac ccttggcaac gtcagtgccg gttatgccca ttgaaacacc
      721 tatatccgcc ttcttcaaag ccatggcatc attaactccg tccccggtca tagccgcaat
      781 atgccccctc tttttaagca tttccacgat tctcacctta tgctcgggag atactcttgc
      841 atatacactg gtattttcga ctctgttgaa aagctcttca tcagaaaatt tatccagttc
      901 attacctgtc agtacgccgg aatgctcttc cataagccca agctccgcgg caatagccac
      961 agcagtgtcc ttatgatctc ccgtaatcat aaccggtttt attccggctt ctttacatgt
     1021 ctttatcgcc tcaaaggcct cgaccctggc aggatcaatc attcccataa gcccgataaa
     1081 tatcaggtta ttttctatcg aatccgggct tattgcttca ggcagcttat cgtatttcct
     1141 ataggtaaag gcaagtactc taagagcatc tcccgcaaac tcgctgttaa ccttggctat
     1201 ttgctgcttc atagcttcgg tcatgtcaat cacgctgccg ttttggagca ttttggagca
     1261 cctgcccaat attatatcgg gagcaccctt tgtaaaagac actattttgt tttgaatgaa
     1321 attatcatgg aaagtagtca tcatttttct ttcggaatca aagggtatct cttcaatcct
     1381 ggcatgggct tcattcattt ttgccttatc aaagcctgcc tttgcacctg cgacaacaag
     1441 ggctccttcg gtaggatccc ctattatccc ccagaggttt tccctggcat ctttctgctc
     1501 aagggtggag tcattgcata atactcccgc agaaagcagg agcttcaacc cgggttccgt
     1561 ctccggattg attttttcgt cttcaaatgt aatctctcct gagggattgt atccctgtcc
     1621 cgatatttta tagatatttc catccgtaaa agctctcacc acggtcattt cgttctgtgt
     1681 aagagtaccg gtcttgtccg agcatattac cgttacacat ccaagagtct caaccgcaag
     1741 gagctttctg actattgagt ttctccgcac catcctcttc atccccagtg caagtacaat
     1801 tgtgacaaca gccggcaatc cttcaggtat tgcggcaact gcaaggctta ctgataccag
     1861 gaacatctcc aggatatcgc cgcctctcat aaacccaatg gcaaaaacaa cggcacatat
     1921 tataaggcag gccagcccca gccatctacc cagctcattc agcttttgct gcaggggggt
     1981 tgtttcatct tcaaaagtct gaattgcttc ggctattctg ccgatctcag tatttgttcc
     2041 tgtgttgacc actattcctt tgcccctgcc ataggttact atggtgctca tgtatgccat
     2101 gttgagcctg tcgcctattg aaacctcagt atcaaaggcc ttgcttgcat ccttttccga
     2161 cggtactgat tcccccgtaa gtgaggcttc ctccacttta aggttagagc tttctataag
     2221 cctcatatcg gcaggtatgc tgtctcccgc ctcaataaat acgacatccc cgggtacaag
     2281 gttttttgcg gggactgtct ctatgcgctc atctctaagc acctttgcat ttggagccga
     2341 catcttttta agcgcttcca tggctttctc cgcttttcct tcctgcacaa cccccaggac
     2401 agcatttaca attactattg caattattac aatagagtcg gatatttctc caaccattcc
     2461 ggagactata ctggcgataa taagtattac cacaaggaaa tccttaagct gtctgccgat
     2521 tttaacccat accggttctt tttccttttc cttcagttca ttatagccgt acttttcaat
     2581 cctgccggcg gcttcctctc tggaaaggcc tttctgcaaa tctgtcgaaa gctcctcgga
     2641 aattgcctga atacccttgt tgtaccagtt caggttcatt aaatcccctc catatgataa
     2701 tattttatta tttgtttatc agcctgaatt attacattgc atcccccatt gtataggaaa
     2761 gtatactttc ctatacaata aaaatgcctt tgacgcaaaa acaatttttt gcaccaaagg
     2821 cctctaaaat caaatatatt atctgccaag tatctcaacc agctctacac cgttatttct
     2881 ctccgttacc gtcctgatgg accattcatt ctggaaaagc agcacagggt tcaaggattg
     2941 atcctgtaca atcattgtat cagttgtaat cctaaaggtt tccggattaa attccggatt
     3001 ctttacccat cttatatact tatagggcaa atgctccatg cttatatcca cgccgtattc
     3061 atttttcagc ctgtattcca atacctccag ctgaagtata cccacaactc caataataaa
     3121 ttcctgggtt atggcatctt tttggcggaa taactggatg gcgccttcct ccgatatctg
     3181 ctctataccc ttcataaact gcttgcgctt cagggagttt ttggtataca cccttgcaaa
     3241 atgctccggt gcaaatacgg gtattccttc gaacctgaag ctgcctccct cgcacaaggt
     3301 gtctcctatc ttgaaaattc cgggatcgaa tactccgatg atatcccctg cataagcctc
     3361 ttcaacaatc tccctatcct gggccaggaa ctgctgcggc tgagacagcc ttatcatctt
     3421 gcccccctgt acatggttga cttccatgcc tttggtgaat ttcccggaac aaatccgaag
     3481 aaacgctatc ctgtccctgt gggtgggatt catatttgcc tggattttaa aaatgaatcc
     3541 ggagaactct tccttctccg catctatctc accgatgtcc gcatttctgg aaagaggcgg
     3601 tgttgttaat tcaagaaatt cctccagaaa cacctgaact ccgaagttgg tcatggcact
     3661 gccaaaaaac accggagtca gctccccctt caggactttt cttctgctga actcgtctcc
     3721 cgcaacatcc agcagcatta tatcatcaat aagcttatta tgaacatcct ctccaagaag
     3781 atcagaaaag acaggatcgt caacattgcc tttagaggac cctactatgc tttgtccatg
     3841 gtcaccgccg ttaaacagct ctatctggga cagctttctg tcgtaaatac ccagaaagct
     3901 tcctccgact cttattggcc agttcatagg aaatgactga attcccagca ccttctcgat
     3961 ttcttccgcc agttcaaagg gatccttccc tgccctgtcc attttattaa taaaggtaaa
     4021 aatcggtatt cccctcattt tgcatacgtg gaacagcttc ctggtctgtt cctcaacacc
     4081 ctttgccgag tcaattatca tcaccgcgct gtctgccgcc ataagtgttc tgtaagtatc
     4141 ttcgctgaaa tcctgatgcc cgggtgtatc aagaatatta atgcaataac cgttatattc
     4201 aaactgcatt acacttgaag taacagagat tcccctctgt ttctcaatct ccatccagtc
     4261 ggatacggcg tacttctgag ctttcctggc ttttacagaa cccgcaaggc gaatggcgcc
     4321 tccgtacagc aaaagctttt ctgtcaaagt tgtctttccc gcatcagggt gggatattat
     4381 ggcaaaggtt cttcgccttc tgatttcctt ttgtaattcc tgttgtgcag ccggcattct
     4441 attactcctc tattgttcag tatatacttt tatattttta acttgttcat caaatcccaa
     4501 gtatcacatt ccgtgactta ttaattttac acgaacagca atagagtgtc aattaaagca
     4561 tcaatatacc ggagtgcaga agccggcata tttgggattt ttacctacgg taatttcaaa
     4621 aaacaggctc ttcagcttag tacatatatc ccccggcctg cctgcaccta tagtcctcct
     4681 gtccacttca actatcggca ttacttccat agcagttccg ctgaagaaca cctcctgtgc
     4741 cgagaaaagc tcattccttg ggatgcttct ctctactacc tccattccaa actcttcctt
     4801 tgcgagagtg ataatcgttt ctcttgttat tccctcaagt atattgtctg aaggcggcgg
     4861 ggttaccagt tttccttttt tcaccatgaa aatattttct ccgggccctt cacatacatt
     4921 tccgttatca gtgaggaata atgcctcatc aaatccgttt cgaagcactt ccagggacgc
     4981 cagtgccgaa ttcaggtatg ccgctgatgc cttagtcctt ggaggcagca tattatccct
     5041 gagcctctgc catgaagtaa atgcaacatg catttcctgc ttgcccgaaa aattccccag
     5101 aggctggcag taaataagca gtctgttgtc atcatccaac agtgtaggat tcaacccttc
     5161 accgccttta tatgccaaag gccttatata caccgttgtc ctgaagccgt acagctgctg
     5221 tccttcttca tcccaatatg ctcttattcc ctcaaagcag gcaagcccat aattgaccac
     5281 tttgcttctt atacttatac tcacttcttt ttccggtact atttttccat tgaaaaaaac
     5341 atatgattcg tccataatta catctcctta gtttttctaa aaatcatcta tacactcatt
     5401 tataggctct cccgccggaa ccatcggaag caccaggtca tccggatgta tgatgcattc
     5461 tattacaaca ggctcattca tatcaaaagc ttctttaaaa gccttttcga cttcatcatt
     5521 ttcagtgatt ttcgccgact ttatcccaaa agcatcggca agctttgtaa agcttacttc
     5581 atccgcaagg cttgtatggg aatacctttc gccgtggaaa atcttctgcc actggcgcac
     5641 catccccaga gaactgttat tcaaaaccac ctgaacaatc ggaagcttgt attttgtaat
     5701 ggtagaaagc tcggaaagat tcatcctgaa gcttccgtcc cctgctatat taatcacctt
     5761 gcaatcaggc tttcccactg ctgcgcctat tgccgcacca aggccgtagc ccattgttcc
     5821 aagccctccc gatgatatga atgtcctggg atcggcaaag ggataatact gtgccgtcca
     5881 catctgattc tgccccacct cggtagttat tatactcttg ccttgagtaa gctcataaag
     5941 ctttttcatc aaatattggg gattcaagga tcctttgctt ttatatgaaa caatataagc
     6001 ctctttccag ctttgtatct gtttgttcca gatgccctcc ttcttttcgt caattctctc
     6061 cagtaaaagc ctcagatatt tcttaacatc accgcaaagg ggaatttttg tatctacgtt
     6121 tttccccaat tcctccgggt caatatctat atgaataata ttggcataag gagcaaagct
     6181 gtcagccttg catattgtcc tgtcactgaa ccgtgccccg acagctatga gaagatcgca
     6241 gttgcatatg gcaatatttg agcatcgtga cccatgcatg ccggccagac ctgtaaaata
     6301 aggatgagtt cccgggaata cccccgtacc cattaatgag caagccacag gagcctttat
     6361 cttctccgcg aattccagca gttcccggca ggaatccgaa atcttgacac cgcctcccgc
     6421 ataaatcaac ggcctcttac tgttgtttat caattcaact gccttatcca ggtataagtc
     6481 cgtttcacag ctcatttccg gcagattgga ggtgtcatag ccggtatctg cctccgcagg
     6541 ctcatattct attacagcct gctgcacatc cttgggaata tcaatgagta ccggccccgg
     6601 acgcccggac cttgcaatct caaaagcttc cctgacaatt ttcggaagtg tttccggatt
     6661 cattaccaga tagttctttt ttgttatggt ctttgtaatg cttgtaatat taagctcctg
     6721 aaaggaatcc ttgccaagaa gcgaccttgc cacctgcccc gtgattacaa ccatcggaac
     6781 tgagtctctg gatgcagttg ctattcccgt tacagtgttg gcagcccccg ggcctgaagt
     6841 tgccaggaca actcctacct tccccgttgc ccttgcgtat ccgtccgcag catgagctgc
     6901 accctgctca tgggcagtca atatatgggt tatgtccttc ctttcgtaaa gagcatcata
     6961 aatatttata accgtgcctc ccgggtatcc gaatatcgta tcgactccct gttcccggag
     7021 gcattccaac agtgcctcag cacaattcaa tctcatttca tcacctccaa actaaatcag
     7081 aatgttttct gcaacaatac tgcccatctt ttccgtcccg actaaagtca ttcccggctg
     7141 cattatgtca gctgtcctgt atcctttgtc caaagcatta tttacagcct caaggactgc
     7201 atctgactcc ttctccattc cgaagccgta tttcagcagc attccgccgc tgagtattgc
     7261 agccagggga tttgcaatgt cctttcccgc aatatcaggt gctgagccgt gtataggttc
     7321 aaacaaacag gcttttgagg ctcccaggct ggctgaaggc agcatcccga gagaaccgga
     7381 aagcatagat gcctcatcgc ttaatatgtc cccgaacagg ttctccgtga gtatcacatc
     7441 gaactgcccc ggattcctga taagctgcat tgccgcattg tccacataca tatatgctgt
     7501 ttccacatcc ccataatcct tcataatgct tcctgtaacc tcacgccaca gccttgagct
     7561 ttcgaggaca tttgccttgt caacaatagt aattctcttt cttctctttc ttgcaatgcc
     7621 gaaagctatt cctgcaatcc tttcaatttc acgggatgtg tattccatca tgtcccatgc
     7681 cttgattccg tcctttgtct cgactctttt cttttcgcca aaatatgcgc cccccgtaag
     7741 ctcacgaaca atcattatat caaagccttg tgacagtatc tccgctttca gcggggaagc
     7801 ggttctaagc tgcggaaaaa gcattaccgg cctcagattg gcaaataatt tgagagcttt
     7861 tctgataccc agaagcccag cttccggcct caggttcccg ggaaggccgt cccatttcgg
     7921 cccccctacg gcgcccagaa acaccgcatc gcttcccgcg caaatatcaa cagtctcatc
     7981 gggcagcggc actcccgtct catcaattgc cctgcccccc aacagagctt cccggtattc
     8041 aaacttgcat ttaaatcttt ccgatactgc ataaagcacc tttaatgctt gttcagttac
     8101 ctccggcccg attccgtctc cctttatcaa agcaattttc atagaccctt cccccgtttc
     8161 cttacataat ttatcaatcc ttcggctttc attatttccc tcataaattc agggaaggat
     8221 tcagcctgat aggtccggcc tttggtgata tttgttattc ttcctgtctt aatgtccgct
     8281 tccaccaggt cacccttttc catatccttt gctgcagcag cgcattcaag tatgggaagc
     8341 cctatattta tggcatttct gaagaaaatc cgtgcaaagg actcagctat gatgcacttg
     8401 attccggaaa gctttattac caacggcgcg tgttccctgg aagaaccgca gccgaaattt
     8461 ttgcctgccg caattatgtc tcctggcttc acagccttta caaaatcaca gtctatgtcc
     8521 tccatacagt gagaggcaag ctcagcctta tcggttatgt tcaaatatct tgccgggata
     8581 ataacatccg tatccacatt atctccgtat ttaaaagctc tgccttttat catccgagca
     8641 cctcctcagg agaagaaatt ttgccggtaa tggcggaagc cgctgctact gccggacttg
     8701 caaggtatat ctcactttcc ggatgcccca tcctgcctac aaaattcctg ttggtagttg
     8761 ccactgccct ttcccccttt gccagtattc ccatgtggcc tccgaggcag ggcccgcagg
     8821 tcggggtact tactgccgct cctgcttcaa tgaatatctc aagcaaacct tccttcattg
     8881 cctccagata tatcttctgg gttgccggaa taattattgt ccttacactt gaacttactt
     8941 tgctgccctt caatactttt gcagccgttc tgaggtcctc aagcctgccg ttggtgcatg
     9001 accctataac tacctggtcg atccttatat ctcccacctc atctatactt ctcgcattct
     9061 ccggaagatg cggaaatgca actgtcggtt tgattgctcc caagtcaatt tctattaccc
     9121 tgttgtattc tgcatctgcg ccggccttga aaatctttgg gattctttcc gaatgctccg
     9181 aaacatattc cagagttttg ccgtcagctt caaaaattcc gttttttgct ccggcctcta
     9241 ttgccatatt tgcaatggtg aaccggtcat ccatggaaag gtgcccgact ccgtccccgc
     9301 tgtactccat agattggtac aaagctccgt ccacccctat cattcctatg atatggagta
     9361 taatatcctt tccgctgacc catttccccg gcttgccttt caatatgaac tttattgctc
     9421 ccggcacctt gaaccatgct tctcccgtcg ccatacccac agccatatcg gtacttccta
     9481 ttcctgttga aaatgctccc agggctccat aggtacaggt gtgggaatca gctcctatta
     9541 tgacatcccc cggaactgcc aggccctttt ccgggattag tgcatgctct atccccattt
     9601 gccctatttc aaaataattc tcaatttcca tttttcttga aaactccctg accagtttgc
     9661 agttctccgc agatttaata tccttgttgg gagtgaagtg gtcaggtaca attgcaacct
     9721 tgcttttgtc aaacaccctg tttgccccta tcctgttgaa ctcttcaatt gcaaccggtg
     9781 ctgtgatatc attgccaagc accaggtcaa gcctggcttt tatcatctgc cccggatgca
     9841 cttcctcaat tcctgcatga gctgccagaa ttttttgcgt cattgtcatt cccatatttt
     9901 tttgcctccc ttttctccca ttcccccagg aagtactcta tggagtcaac caaagcgaag
     9961 cagcttgctt ctattatatt tgttgaaacc cccaccgtac cccaaacgaa ttcaccgtct
    10021 gtggattcaa tatgaaccct tacctttgca gccgttgcct gagccgtatc aagcacgcgg
    10081 accttataat ccgttaatct gactcccttc agctgcggat agaacagctc caaagctctt
    10141 ctcaaagcct tatctattgc gtttacaggg ccgttgccct ctgcagcact gatttcctca
    10201 acccccatta cttccacttt aattacggca cctgcgctgt ttgattcatc ccccttatcg
    10261 tcacaccata ccctgaagtt aataacctta aaaaactcgt tatcctttcc aagagacctc
    10321 tttatcagga gttcaaaaga gctttcagct ccttcaaact gatatccctc atgttccagc
    10381 ttttttagct tatctgttat ttcctttact tcggaagaat ctttggaaag ccccggagca
    10441 tacttctgaa tttttttaag tatcgtactt ctccccgata cctcagacat cagaaccctt
    10501 ctttcatttc ctacaagctc cggcgcgata tgttcataag agaccggatg tttcaaaact
    10561 gcgtctatat gcattccccc cttatgggca aaagcattgt ttcccacata gggcccatat
    10621 tcattgtggc ttatgttggc aacttcactg acaaaccggg ataattgcgt aaggcttttt
    10681 attttgcaat catcaatcaa gcagtatttt cttttcaatt gaagatttgg gattatagtg
    10741 cacaggtttg catttccgca gcgttctccg tatccgttaa tagtcccctg cacctgtgac
    10801 gctccgtttt gtacagccat tacactgttt gccacagcca ttccgcagtc attatgggtg
    10861 tgtatgccta ttggggtttc caggatgttt gagatttttc cggtaatatc ccctatctcc
    10921 cagggaaagc ttcctccatt ggtgtcacat aataccagcc agtcggcacc tgcatcctgg
    10981 gctgccttta aagtatgaag ggcataatcc gggtttgatt tgtacccgtc aaaaaaatgc
    11041 tccgcatcga aaatgacttc cttacccttt tctttcataa atcctattgt ctcgcttatc
    11101 atcctcaggt tctcatcaag ggtggtacga ataatgctgt ttacatgaaa atcccagctt
    11161 tttccgaata ctgcaattgc aggagtttcc gcccttaaaa gcgacagaat gttgtcatcc
    11221 tcatgaacct ttgtatttgc tcttctggta ctgccaaaag caaccagttt cgaattcttc
    11281 agtttaacct ttctaatcct gtcaaagtat tccatgtctt ttggatttga tcccgggttg
    11341 cctgcctcta tatagtcggt tccaaagtcg tcaagcctca tggtcaggtt aaccttgtcc
    11401 ctcaatgtga atgaaatacc ttccccttgt gctccgtccc ttagagtcga gtcatatata
    11461 tatatcttct tacaataatt ccaattccca acgctcatca cctccggccc caaaataatt
    11521 ccggctcttc acaaattcaa aaccctttac ttcgctctct tccggctctt caacccttga
    11581 ttccatgttg tttattgcat ttatatatgc caatgcgctg gcttcaacta tatccgtgct
    11641 gactccataa cctaccgacc ttgttttatc ctttgaaatc tttacggtaa cttcgccaag
    11701 ggcatcttta ccttccgtag aagcctttat cacgtattca tcaagcttta cccttacacc
    11761 tgttgccctt tcgatggcat tgaaggtggc atcgacaggc ccgtctccgc aggcggcttc
    11821 ttcgtacaca tcatcattgc atctgattct gacggttgaa gttgaagtta tgtcacttcc
    11881 gcttgatacc tggaagtatt ccatcctata cttttcataa gccttccttg attcctgggt
    11941 aatcaacgcc tcaatatccc tgtccataat acatttcttc ttgtccgcca aatccttaaa
    12001 ctgtataaat gccttgttta tctcctcctg gctaaggctg ctgtaaccca tttccttgag
    12061 tttttcttcg aaggcatgcc ttccggaatg ctttccgagt accatcttac tctgcttcag
    12121 ccctatggat tcgggagtca ttatttcata ggtctcgctg tttgacatga ttccatgctg
    12181 gtgtatcccc gattcgtgag caaatgcatt aacccctaca atcgctttgt tgggctgcac
    12241 atgaaccccc gttgcaaagc tgaccattga acttgttctg taaagctttt cagtaactat
    12301 tccggtataa acattataaa aatccttcct ggtcttaagt gccatgacaa tctcttccaa
    12361 tgcggcgttc cccgctcttt cacccaatcc gtttatagtg cattccacct gatctgcgcc
    12421 gttttccacc gctgccagag aatttgccac agccattccc aggtcattat ggcagtgcac
    12481 gctgattatt gccttgtcaa tattgcttac attattcctg atactgcgta taagccctcc
    12541 gaattcgaca ggagttgtat atcctactgt atcaggtatg tttactactc ttgctccggc
    12601 attgattgcc tcctcaatca ccctgtacag aaattcctta tccgtccttg cggcatcttc
    12661 gggagaaaat tccacttcag gacacaggtt ccttgcataa gcaaccatct ccgccgtttt
    12721 ttccagcaca tcttccggct tcattttcag cttgtatttc atgtgaatgt ccgaggtggc
    12781 aatgaaggtg tgtatccttg gccgctccgc atattctatt gcttcccacg ctgtattgat
    12841 atcctgcctg acagcccttg ccagggctgc aattattgaa cttctgacat tctttgctat
    12901 agactttact ccttcataat caccctttga agcggcgggg aatccagcct caatggcatc
    12961 aactcccagc tcgcttaact gccttgctat cttaagtttt tcattactgt ttaagcttat
    13021 gcccggggat tgttccccgt cccttaatgt tgtatcaaaa atatagatcc tcttcgacat
    13081 aagaattcct cccatacccc tattattttt tgttaatcca cgacatcatc tttctgagct
    13141 cttttcctac ctgttccaac tggtggcttt gctctttctt tctcattgcg ttgaaggaag
    13201 gtctgttaac ttggttttcc aataaccagt tctttgcaaa ggtgccgtct tgtacttcag
    13261 ataatatctt tctcatttct tttcttgtat cttcagtgat tattcttttc cctgaaacat
    13321 aatcaccata ttctgcagta tcgcttattg aatgcctcat atagccaaac cctccctgaa
    13381 ttatcatgtc tactatgagc ttcatttcat gcaggcactc aaaataggca ttttcaggtg
    13441 cataacctgc ttccacaaga gtttcgaagc ccgccttcat aagctcggtt acacctccgc
    13501 aaagcacagc ctgttctcca aagaggtctg tctcggtttc atccttgaag gttgtttcca
    13561 gcactcctgc tcttgtacct ccgataccct ttgcatatgc aagggcattt acctttgcgt
    13621 ttccggaata gttctgatgc acagctatca gacatggaac tccttttccc tcctgatact
    13681 gacttcttaa tagatgaccc ggactcttcg gtgctaccat gaatacatca atattttccg
    13741 gtggaactat ttgcccataa tgtatattaa acccgtgagc aaacatcaat gactttccct
    13801 ctctcagatt aggttctaca gattctttgt agatctcagc cgacttctca tcaggtgtaa
    13861 ccattatgat aaaatcgccc tttttaacag cctctgcgac ttcatatact tcaatgccat
    13921 gagactctgc actttcccaa gacttactgc ctttgtaaag gccgacacat acatcaactc
    13981 cgctatccct tagatttagt gcatgtgcat gtccctggct tccaaaacct ataacggaaa
    14041 ccttcttacc ctttagtacc tctagatttg catccttttc gtaatacatt tttacatttg
    14101 acattttaca tcctcctaaa aaattattaa tataactcaa aaagactttt caccccatta
    14161 ctggagcgaa aagtctttcg cggtaccatc caattttctt ctctttttat ttaacggctt
    14221 agccggcggg gcttactgct atttcagcct gcaactccag gatgaagact ttcaacaaat
    14281 tcatcctatg gattcacacc agcctccact ctctgaaaga attaaaatct gtttacttat
    14341 tcctgtcatc gtttttgatt ttattaagct gcttaatatc tgtatcacgc taataattat
    14401 aattaggctt ataatcagtc ccacgacaaa tagaattatg cctgaaacca atagcacttg
    14461 gctgcttagc agcagtacaa ggactgataa taggataatt ccaaaaataa tatatgaaat
    14521 tgtgctgtta aaccgtactc tgtccatatt gatccctttc atcttcacaa tttcctttcc
    14581 gcctttaatc ggcagaaaaa tatttaaaga aaatgatacc agcttttaaa gcatatgtca
    14641 aatctttttt tattattata ttattatatt atgggttgtg ctataccaaa cttctttgaa
    14701 agtagattca ttatatgtta tatttgttac atagtattcc ggtaccctga acacattata
    14761 gctgcaaggc actattaagg aggttttgct tatgaacaac tttactgatt atcttgatat
    14821 aaacagggag gtgcttgacg gcctcagata ttacaagccc gttgttgctc tcgaatcaac
    14881 cataatttcc cacgggatgc catatccgga taatattgaa accgctctgt ccctggaaaa
    14941 aattatccgt gaaaacggag ctataccggc aaccatggga ataataaagg gaagaataaa
    15001 aataggcctt gaccgtgaag aaatggaatt cctggcaaaa gcaaaggata ttatcaaaac
    15061 aagcaggcgt gatattccct ttgtggtagg aatgggctta tccggaggaa ctacagttgc
    15121 agctacaatg attttatcgc agtatgccgg aattcgtgtc tttgccaccg gaggaatagg
    15181 aggcgtacac agaaacgcag aaaagagctt tgacatttca gcagacctta cggagctttc
    15241 aaatacaaat gttgcagtga tatgcgccgg tataaaggca atacttgata tcgggcttac
    15301 ccttgaacgg cttgagacct tcggagttcc ggttatcgga taccgtactg atgatttccc
    15361 tgccttttat accagaagca gcggatttaa gatccccatg agagcaggca gcccacggga
    15421 atgtgcgaaa ataatgaaaa caaaatggga tctgaatctg gacggaggaa ttgtagtggc
    15481 aaatccgatc ccgaagcaat atgaagctca gaaggataat attgataaag cgatagaact
    15541 ggctcttttt gaggcagata aaaacggcat taccggcaag gcagtaactc cgtacctgct
    15601 gggcaaggtc aaggagataa cagccggaga aagccttgca gccaatatag ctttggttag
    15661 gaacaacgct ttgctggctg ctcagatagc agtcgaatac agcaagctgg aaaatggcag
    15721 ctaaagctgc agatacgaga cacgagatac gagattccgg ggttacgggt aacacagagg
    15781 acacagattt cccacggatt cctgcggaat aacacagcaa ctattatatt taatatagtt
    15841 acctcacgga atttagatac ttacttaatt atctgtcatt atctatgttc aaaattttcc
    15901 tctgctgtgt atttagtggt tttctgtgtt tttagtgtta accgttacca actacgcatg
    15961 tttctattat tcaaatagct tcctgatccc ttcatcatag ggctgcctta caactccctt
    16021 ttctgttatt atagccgtaa tcagctcatg gggagtcaca tcaaaagccg gattgtaggt
    16081 ctttaccccg agcggggcag tccttctgcc caggccgcat gtgatttcgt caggcttcct
    16141 ctcttcaatg ggaatctcat tcccgcttgg agtttcaata tctatagtcg atgtgggccc
    16201 tgctgtataa aatggtatat tatgcgcctt tgcaagtacg gcaaggccat aagtgccgat
    16261 tttatttgcc gtatctccgt tggccgcgac tctgtcgcag cctacaatca ccgcatctat
    16321 caaacccttt gccattaccg ctgcggccat attgtcgcag ataagggtta catccactcc
    16381 ggcctgcata agctcccagg cagtaagcct tgccccctga agcagaggcc ttgtctcatc
    16441 tgcaaatact tttatatccc aacccttctc ctttgccaga tatatcggag ccagggcagt
    16501 tccgtacata gccgttgcca ggccccctgc attgcaatgg gtaagtaccg tcattccatc
    16561 atgaaacagg gtaagtgcat tctcgcctat cgctctgcac atactttcgt cttcatccct
    16621 tatggcattt gcttccctga taagtatttc cttcaactct tccacggttt tatttttatt
    16681 gtcaaatgct gctctttcca ttctcctcaa tgcccagaaa agatttaccg ccgtaggcct
    16741 tgaacttgca aggtattggg aattcttttt taattcagtc ataaattcct catggctttt
    16801 agcccccgta tcccgcattg cgacacacag cccgtaagca gcggctattc ctatggcagg
    16861 agcgcctctt accttaagat gaagtatagc atcccatacg tcctccgcct tgtccagctt
    16921 tataaattca gttgtatcgg ggagctttgt ctgatccaat ataatcagtt cattgttctc
    16981 ccattttaca gacctcatgg cttcaaagat ttttttcata aggcactccc ttgttgtgct
    17041 gcttccacaa catgcttcag aagcatcaat gttgtaacag cacctacccc gcccggtacg
    17101 ggggttattt ttgatgcaat atccttcacc ttttcaaagt cgacatcccc gcatatcccg
    17161 ccttccggcg ccccattgac tccaacatct ataacaacag ctccgggctt tatatagtca
    17221 gcccctacgg cccttgcttt tccaagggca gccactacga tatccgcacg ggaaacaatg
    17281 ctgcttatat cctttgttct ggaatggcat atggtcacag tggcattctc tttaagcagc
    17341 atcatgctca gaggctttcc cacaaccatg cttctgccga taacagccac attctccccc
    17401 tggaggttta ttccgaaaaa cctgataatt tccataaccg ccgcaggggt gcacggctca
    17461 aagcccgagt cgtcgccttc aaataccttg gccagattgt aaggattcat acaatctata
    17521 tccttggcag ggtttatcat attccttgcc ctatcctcgt ctatatgtgc cggaagcggc
    17581 ctgaacacca gtatcccatt tatgcttctg tcgctgtttg cggcttcaag ggccttcatg
    17641 aagtcctcat tgcttatatc atcagaaagc gccgtcactt cacagcttat tcctatggac
    17701 tcacaatttt ttattatccc cttttcataa gaaatatcct cagggcgtgc tcctactctg
    17761 attattccca gcttgggctt caatccttca gcggcaagaa gctgtacttt ttttgccagt
    17821 tcttccttaa tgcttcttgc agcctctctg cagtctaata cagccatatg tccattactc
    17881 ctttattaaa atatatttta tctaaattca ttaaagcact ctatcattgc cactgcaatc
    17941 ttaaatgcct gttctctgct tgccctgccg tagctgccct cctgtccttc tttgggggca
    18001 ggcccgaaca tgttatttcc aatgccggtt atgatattgt tttttaccat gaaatagaca
    18061 gaatccttgg catagccgct tataagttcg tcgtcactga atttggccac tccggctgtg
    18121 tccagaacat gccggtcata ggccgcgtct tctcccaatg cccaaccttc ccagtaaacg
    18181 gctttgtaaa ctcttgtaag catggtgcag gcctgctctc tggtcaagct cgcatccggg
    18241 gcaaacagcc cgtttccgat gcccgacaca attcccaagc tgtatgcctt gattacgtca
    18301 gaatcactgg tatcggtgaa tggactggca aggtcaactg caatcttact gcccttaaga
    18361 ttctcataaa gcttcactgc cagggcgctg aattccttcc tggtgatgcc cctggccata
    18421 tcctgattca ggaggatatc agggattatg gataacctat ccgccttgtc catccattcg
    18481 caggcccagt ctgaagtgtt gagccacttt ccttctcttc cgtttttata ttgcataaaa
    18541 tcagggattt gatatccgaa agtccattca aatgaaggct ccgcaccttt tgtccatttg
    18601 agatagcctc ccttctgcat atcaacccat gcgttgagag ccgctgatat gttgtcgtac
    18661 tgcctgcctc ctatgcttac gggagaagag agctttcctg tagctgtatt cactgccttg
    18721 gaagtaaccg cagctttgtt aaaaaaagga taagcaggaa atgggctgaa cttggaatcc
    18781 caatagcagt aattatatat gccggttttt gcccccagcc ttatattggc aaaagcatct
    18841 ccatcctctt cattcttttt ccctgctttt aatctgacag taccgctgaa atagaagtta
    18901 ttggttggaa cctcctctcc aattcccata acataaagtt caccttcgga atcggttgtg
    18961 cttgcatata tccttcctct tgaataggcg ttttctatag ttacttctct tcccggcttt
    19021 gttatgccaa tggcaagttg tatcctggca atttttgttt ccacattgcc ttcatttccc
    19081 acatttttca gctcggctat ttccgactct cccgcgatac cgcctgccat aatctcttca
    19141 ttcgcataag ggccggaatg ctcaaaatat atactgcctt tattcagagc cttatccatg
    19201 ccggagtccc tcagttctcc cactatgccc cctgccgcag atcttgaact gttgggagcc
    19261 cgtgtctgca taacagtgac atttccctga ttcaacagat tttctgcttc tgcgccaaat
    19321 ccgcagaatc ctgccaggcc gcctacggca cctatgccgt caaaagtcgc cgtaatattt
    19381 ccctgattgg tcaggttgaa aattccggcc tcctttccca ttgcatctcc ggttataccc
    19441 cccatatata gtctgtcacc accgcttatt tttatggtga gatcagcttt gttgatcatg
    19501 gaatcaatcc tgcccaaaag gtgtcctgca gctccgccaa aatagaaatt ccccgggacg
    19561 ttaacggaca aatccacact tccttctgtt atgcagtttt caatgcttcc ttccgcccag
    19621 gctgcaatca aaccgaagca ggcctcttct tcagatgata tggccttact gatctgcccg
    19681 ctgaccgtaa gatttttgat ggaggatctc ctgtccgtac cgccgaacaa gcccatatta
    19741 cggctgtcgg caatatttct caaagttatt gtttttccat taccgtcaaa agtgccgcta
    19801 aaagaaagcc cgttgacgcc gacagggttc catgtgtttt cgcccatatc tatgtttccg
    19861 gtgagcttat aggatgcctc catataaccg gcattccatc caacctcaga aaaagccaaa
    19921 aggtcttctg ccgtggatat aaggtaggga tcctccggag ttcccgcgcc tccgctgaag
    19981 gaaggcattt tgccgggttc ctcccggcag tatgcaggaa tcaggcttcc cccaatgaaa
    20041 agaacaatca cgattaaaaa tataagcttt ctgaatcggt ttttgctttt attcatctct
    20101 taccctcctc actttccgat ccctttgggc cctttgaagc ccttacagca tgactttata
    20161 ccattgcagt aaaattttat gatataatat tctcaagcgc atattataat attattctac
    20221 tatatcacta ttttttttac caggaggggt taaattgaat acggacagaa tgatcagtga
    20281 atttattgaa atggccaagg taagcagcct gtcgcttaag gaaggcagat ttgcttcgct
    20341 gttggctgaa aaactcaggg gtttggattt cgaggtatat atagacaaag caggtgagct
    20401 ggcgggagga gataccggaa atgtactggg caggttaaag gggactcttg acactcagcc
    20461 gatactctta tgtgcccata tggatacggt agtcccggga gaaaacataa accctataat
    20521 ccgtgatgga atagtataca gcgatgggac aagcatactg ggaggcgatg acaaggcagg
    20581 aattgctgcg atattggaag cagtaagata cataaaagag aataacatac ctcacggtga
    20641 cattgaggtt gcattcacaa tttgcgaaga aaacggaatg ttcggtgcca agcatctcga
    20701 ttacagctgg ctcaggtctg aatttgcctt cgttcttgac agcagcggag ccgtgggatc
    20761 agtaattgta caggccccgg cacagattaa aaccctttcg gtaataaagg gaaaagctgc
    20821 ccatgccgga ttggctccgg aaaaaggcat cagcgcaata caggcggctg caagagctgt
    20881 taacaatatg aagcttctcc gcatagacga agagacaacc gcaaatatag gcaggataaa
    20941 cggcggcggt gccaccaaca ttgtatgtga caaagttgaa atggaaatgg aagcaagaag
    21001 cttgacaagg agtaaagtcg aagcccaggc aaagcatatg ctggagtgca tagatgatgc
    21061 ctgcagggaa ttcggtgcag aacatgaaac aacacagcag ctgagctaca ccgagttaaa
    21121 cattccgaag gatgcggaag taataaagct tgtggactcg gcaataaaaa gaataggcct
    21181 tgaaacccgt ctggagtcaa caggaggcgg cagtgacaca aatattatga gcggcaaagg
    21241 catacaggca gtaactctgg gaataggaat gaccaatgca cacaccactt cggaatacat
    21301 tgcaataagg gatatggaaa aaaccgcgga attagtcgca tcaataatac aagaggctcg
    21361 ttagagcctc ttgtcattta taagcttttc cactatgggc agatctgccg gtacaaaatc
    21421 aaagcccgga agttcatcct tcgtcaccca tttaaagtca ttgcattcaa ttgcttttcc
    21481 atcccctttg attatcctgc aaaggtagca cagaagcagt atgtcttttt cttcatattt
    21541 gaacttcaca accttataga tatcatcaac ttcaatatct atatccattt cttcctttat
    21601 ttctcttatg atgcactctt cgggagtttc atcgggctcc agcttccccc cgggaaactc
    21661 ccacttgtat tccatgtggg agccctgctg ccgctgtgca ataagcactt tcccatccct
    21721 gattatggcc gctgccgtta caatatacat attacacctt cggtccggcg ttgactatat
    21781 cttcagacac gcccctgaat ttccggaagt tagcattgaa atccgctgca agcttcagag
    21841 ctgtcttttc atattccgcc ttatcctgcc acacattcac cggcttcaga acctcagccg
    21901 gaactcctgg gcattcggca ggcacagcca atccgaatat cgggtcggta tcgtagccga
    21961 cattatccaa ctctccgctt attgcggcat ttaccattgc tctggtgtag gacaatttca
    22021 tgcgttttcc tactccgtag gagcctccgc tccagccggt attgacaagg aatacccttg
    22081 cgttgttctt gtcgattttt ttacccagaa gctctgcata aacctgcgga ggcatcggca
    22141 taaaaggtgc tccgaagcaa gtggagaatg ttgcggtagg ctctattatc cctctttccg
    22201 ttcctgccag cttgcttgta tacccggaca tgaaatggta cattgcctgc tcttttgtaa
    22261 gccttgcaat cggaggcagc acgccggtag catcagccgt aaggaatatt accgtagaag
    22321 gattgcctcc cactccttct tcaacagcat tatcaatata agttacagga tatcccgcac
    22381 gggtattctc ggtgaacttt tcactgtcat agtccggaac ccctgtctca ggatccagca
    22441 caagattctc cgttaccgca ccaaacctta tggcattcca tatctgcggc tcgttttcct
    22501 ttgaaagcct tatgcatttt gcataacagc ctccctcgaa gttgaatacc ccgtcctctg
    22561 tccagccgtg ctcgtcatca ccaataagcc ttcttccctt gtcagcggac aaagtagtct
    22621 tccctgtacc cgacaatccg aagaacagcg caacatcacc gtcttctccg atattggccg
    22681 aacaatgcat aggcattact cccttcattg gaagtatgaa gttcataatc gtaaaaatgg
    22741 acttctttat ctcgccgcag tacattgatc cgcctataat aacaattttc tttgcaaaat
    22801 tcagtataat aaaggcttcg gaattcacac cgtcagtttc ggcatcggca tgacagcccg
    22861 gtgcggctat aaccgtgtat tcaggctcaa aaccttctaa ctcgccttcc tttggcttga
    22921 tgaaaagatt ctgggcaaaa agattctggc ttgcatattc atttatgatc cttatcggca
    22981 tcctataatt cttgtcagcc ccgacaaaac cgtcaaatac aaaaagctcc ttgtctttta
    23041 tatattcatt aattttatta tacaggtttt caaatttctc accgcttatg ggtacgttga
    23101 cctttcccca atccaccttg tcgtgtatgc cgggatcatc aactataaat ctgtcgcccg
    23161 gggatctgcc tgtatatttt cctgtgttga tgctcaaagc tcctgtatcg gtaagagcag
    23221 ccccttcttt tttcagggca atttccacaa gcttccctac agtaagattg cgatgcacct
    23281 ttgcaccaag attatccaaa ttcatttcgg cacctccaag tataaaacaa atatctatgt
    23341 atgctgcaat ctgaattgca aataatgcaa aaactcagcc ataagcatat cataacctat
    23401 attatatatc attattgaaa tttgcgccat gaattttgaa tacaaaaagt gatagatttc
    23461 aatcctctat tcccgttttc agtatttcat agaacttatc catatcaagg tttgacctta
    23521 ctgtctcagc aagcttgtcg tactggcctt ccttatacac ccaataatca ggagccaggt
    23581 tttccaaagg aaccaatccc ttccttctcc ggatcagatt aagaaagctt cttgtgaact
    23641 ccgaactgtc aaatatgccg tggatatatg tgccgaatac cgtaagatcc ttatttatca
    23701 ttccgtcccg tccaaaaact ccctttcccc ctctttcgct aatggttata aaggagtttt
    23761 ccgcatctgt gcccgcagtt tctcccatat gtatttcgta gcctcttact tcagctccga
    23821 atatattgtc cctgcccttt gaaagagtcg taaatttgtt ctttctgaac tccgtaacgg
    23881 cgttaaaaag gcccaagcct tccgagcgtt cattgtccga ctcggcctta aatttatccg
    23941 atatttcttt cccaagcatc tggtaacccc cgcagattcc aaaaacaacg ctgcccctgg
    24001 aatggcattt cagtattttc ttgtccatcc ccgtattttt cagtgcaagc atgtcatgaa
    24061 tagtgctttt tgtgcccggc agtattataa catccggctc cccgagttct ttgtcaattt
    24121 ccgcaaatct cagggataca tcctcatgaa gccttaaggc gttgaaatca gtatgattcg
    24181 acatataagg cagccttatt accgcaatat ccagctctcc gcccttctcc ggcccggact
    24241 tcagccattc ggaaacgctg tcctcatcct ccaggttcat gctgaaatac ggtattaccc
    24301 ccagcaccgg cacattaata atttcttcca gcatcttgat tccgggcttc aatatctcct
    24361 ggctgcccct gaatttattt attattacac cttttacaag ctttttttct tccgggctta
    24421 aaagcagcat tgtaccggcc agggatgcaa atacaccgcc cctgtctata tctcccacaa
    24481 gcagtactgg agccttggca attttggcca tacccatgtt tacgaaatcc tcatgattaa
    24541 ggtttatctc tgcggggctt cctgcccctt ctatgacaac aatatcactt ctatcctcca
    24601 gcctccggta aatgtctgaa atcctctcct taagccttgg cttgaagtta aaatactcaa
    24661 cggcatccat gtcagcataa acaccgcctt caattataac ctgggacttc ctgtccgaca
    24721 caggcttcag aagtatagga ttcatgcaag cttcgggcag cttcccgcag gcctccgcct
    24781 gcatcacctg agcccttccc atttcgtgcc cttctattgt tatatatgaa tttaaagaca
    24841 tattctgaga tttaaaggga ttcacattat atccatcccg ggcaagtatc ctgcagattg
    24901 cagtgcacat caggcttttt cccaccgagg atgcggttcc ctgtatcatc aggctcttag
    24961 acttcaattc ccttttcgcc atgttaattc cctttccgca gcattgcttg gtacaacgca
    25021 gtattgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg agcgtacatg
    25141 tcagtacgtg aggacgtttc ggccgcagca caacgcagta ttgcgccgct tatagacatt
    25201 ctttacctgc cgctgttgat tcttcgtata atacagcgtc cctcttcatt aatctcataa
    25261 agactgtatc ttccctgctc cgcactaaag ctccagtatg catcttccgt aagcccgaga
    25321 agcaccaggg gtattatcct catggtgccc tgatgggata caatcagcac cttttttccc
    25381 ctgtactcct tcaaaatatc ctcaatactg ctctttacac ggatatatat ttcaaagaag
    25441 ctctctcctc cgggaggggc tgattttctc caatcgctgc cccacttttc cagggcttcc
    25501 ttgtacctca ccttcaattc cctgtggtgc tttccttccc agtcaccaaa gttcagttcc
    25561 ctcagccggt catccttaat aatcatatcg ggggtatatc cgcttacaat agaagcggtt
    25621 tcaaccgccc ttatcaggct gcttgaaatt acggcatcaa attgcacatc gtgaagtata
    25681 tcagccaggt cttccgcctg cattatcccc ttttcactga gtcccacatc ggtccagccg
    25741 taatataccc cttttatgtt catgtcggtt tcaccatgtc ttattaaata tatttcagtc
    25801 ttgctgcctt tcatatgatt ccccttcctg ccatgactgc cgttataagg atgaaggaca
    25861 cttccaagat ctcatttgct gctcccaggg tatcccccgt catcccctgc agctttgaat
    25921 atataaagct tctgtacagg aagcatataa ccgtacaaat gccaaaaaca accaaacccc
    25981 aaatatgacc aaagcccaga attattgaaa taccgattgc tgccgcaagc accgttgctc
    26041 ccatggtctg cttctccaaa taaaggcctc caaggccggg gcctgaacgg gcatatgccg
    26101 aggccttcat aagcaatacc atcagggtct ttgccgcaac aggggacaaa agcaccgccc
    26161 cgtacagcgc ctttcccgtc agcatggcaa gcagtgacat tctcagcaga taatcaaaaa
    26221 tgatggctat agtcccattg gtgcctatcc tgctgtctct cattatctca agcattctgt
    26281 ccctgtcccg tgatgagaaa atgccgtcac aggtgtccgc aaggccgtcc gcatggaggc
    26341 ctcccgtaat ggcagtacct gcaataaggc agcatatcac ggggaatact cctcccaaga
    26401 tgtttgaggc aattattagt atcgtcagat taaatgcacc tataatcaac ccgacaatag
    26461 gaaaaaaagc aactcccttt gcaaaatcct cttcagttgc cttcaaattc ttatttacag
    26521 atattctggt caagaactgc agggtgatta aaaaaatcct catatccgtt cctccatatc
    26581 acttaagctt cagggggatt ccgcatacag tgatataaac ttcatcgcac cgttcagcta
    26641 tgtattggtt catccttccg gcaatatccc tgaatgctct cgccaagggg ttttcaggta
    26701 caatcccgct tcccagctca ttagtgacaa ttataacgga agcctctgaa tttccgatac
    26761 ccgccataag ctccgcaatc tctctttcta tttccttttc cgccttttca aaatcctcat
    26821 ttgaacaatc attgccttcc ataccggtat attccaggaa aaggttggtc accattacag
    26881 taatgcagtc aagcaatatc cctttatatt ggctgccctt ttcagatatc actcttccca
    26941 gccctttaca gccttcaaag gtatcccatt cccggggcct tgattcctta tgccttttta
    27001 ccctcagttt catttcctcg tcaaagggta ttgacgtggc tatgtacaga acatttcccc
    27061 caagctcctt tgccagcttt tccgcgtaac tgctcttgcc gctccgggcc ccgcccgtta
    27121 ttaatataga tttacccata aaccaattat ctcctcatca gtataagtat tcatacacaa
    27181 acttataaat ggctagaagc aagcaagata aggcgtgcaa ggtgaaatgc ccggagcgta
    27241 cattaagata cgtgaggaca ttgaatccga agcataacgc agtattgcgc cgcttatcgc
    27301 catttatttt cttatatcta tcaagaagtc atccttaatc gtcgcttcct caaaggttgc
    27361 catatcggat atgattgctt ccgccgcccc tattatattg aaggcaaggg ggcagccgct
    27421 gccctctccc agcctcatat taagatccag caaaggctca agcccaattt cattgattac
    27481 caggctgtac ccgggttccg ccgatagatg ggacggaata atgtaatccc ttaccagggg
    27541 gttgatccta tatgctgcca gggcagctgc cgccgaaata aaaccgtcta taacaatggg
    27601 caccctgcaa tatgccgctc caaggaagca tcctgtcata cctgcaatat caaagcctcc
    27661 gacttttgaa agcacatcga tgggatcata aggatccggc ttgtttatgc ttattgccct
    27721 ctcaattaca gacttcttac tggcatagcc ctcttccgtc aatcctacgc ccttgcccac
    27781 cgcctcttcc gcgctgcagc caaggaaggc cattgcaaca gcacttgccg tgcttgtatt
    27841 tccgattccc atttcaccgg ttccaaacag gttgtatcct tcctttacaa gctgtgaaac
    27901 catttcaatc cctgtctcta tggcagatat cgcttcttcc ttgctcatgg cagggccttt
    27961 tgccatattg gaggttcctt ttcgtacttt tctgtttatc aagcccggat attccaaatc
    28021 gcttccgact ccgatatcta caactctgat atcagctccc gcatgcttag acagcacgca
    28081 tactcctgta atacccttca gaaaattaat tgtcattaat gcggtaactg cttgaggtgc
    28141 ggcacttacc ccttcctccg ccacaccgtt gtctgacgcc attataattg taaccttttt
    28201 ctcaaaactg ttcttttgtt ttccggttat tccggaaatc tttaccgcaa tgtcctccag
    28261 ctttcccaga ctgccaatgg gctttacaag cttgtcaagc ctttccgccg cttccttctt
    28321 actttcttca tatgagggcc tgattcctgc cgctgtcctt tcaaaaatct ccataatgca
    28381 tctccttaat cagtatgaaa taaaaaaacc cgctgtccaa cttaatcgag ccggagcgga
    28441 aaatattaac ttaataatcc tcctcccctc cctccgaagg tagaaaatgt atcataggca
    28501 ggtttcctgg cttgtggctc tccttactct ccggccttct cgcactacaa tggcttgtcg
    28561 gatttcatac ccacttacag tagcgggggc tgcagcggct ttgaaccgct ttccctttta
    28621 acccgttagg gcaccatatg attgtattcg attgacatat tcattatata ttttaagaat
    28681 tatccagtca atgcataata tgcattttat aaaaccaacc ggcattaaaa atgcgtgtta
    28741 caacgcattt ttttcgttta attcacccaa atttaatatt tttagactta gctgtcacat
    28801 tatttgtgca gtttaatatt atgatatcag aggtatatgt aggagacaac atcagacacc
    28861 ggtacatctc ctgcaaatag atttatagct cagtagcaat gcaggaaatc aaatctatta
    28921 tcctcaaata tttaggaggt gaattaaatg ggcaaagagc acagacgcga taaagaattc
    28981 agaataaagc actttccgga taacataagg gacttgctgt gcctgctggc agataatgaa
    29041 gacaacaggg ctactataat actcaatact tgtgatgatt gtgttcagga aaatgtagaa
    29101 gtagttgcag taataggaaa tcttgcagtc ttccgctgcc atgacaaatt caaattcgta
    29161 gatatccact gcatttgcga agtaatcgtt gactgtgaga caatattaag tgaactcctt
    29221 gaatcaaaga gtgaatgcaa atgttagcaa ataggaggga ctcccataat tagggaggcc
    29281 ctcttattcc tttcttacca ccaggaaggc cgtttgcctg ccctcaggtc tgcagccttc
    29341 aaacctccgg tgtattcaaa gtgaggcttg tcaactatgg acttccaatc tccgccccat
    29401 gtcagtccca gctttttgcc ctctattccg ataatcctcc aatagcggtt attattccat
    29461 acggcttttc ctttgacaat cggtaccaca tcataagcaa gcccgaagcc atgagggcct
    29521 atcagccgca tatttgttac tatattgccg ggccttgtac ggccttgtga atacagatac
    29581 tcctgatatt ccgaatctct cagggtattt gtcacaatta tttggactcc ctgcctcttg
    29641 caagcttcga tatgttttct gcatatttcc tgtactaccg ggtgaagatc ctctatttta
    29701 cggctattag tcactttttt caccacctga tatatgaatt ggagcatgta cacctcctac
    29761 ttcatatata tgagaataaa aaagttttgc aatcaatatg actgcaaaac ccttaagttt
    29821 atgccggatc tacttatctg ataccagtgc cgccactctc aaaatatcct ctgcggattt
    29881 ctcaatatca tcgtaggtgg tcttatatga gcatacgctt atcctcataa ccgatttccc
    29941 cgaccatttc gaacctccca gccagcagac gccggattcc tggactcctt taataagggc
    30001 ttccgttttc cggtcattat cgtaataaac caatacctgg ttgtagacta catcgttcaa
    30061 aatctgaagc ccgccttcct caagcagtct tgaaaaatac acggccttgc aatgaagctc
    30121 ttcaaccagc tcagctgcgc ctttcctgcc cagccctttt aatgttgccc acaaatctat
    30181 tgctctcgcc cttcttgaca tctcgggagt atatagcatt ccatccctgt tttcattata
    30241 tattatgtag gaccctgtca tatgcattgc atttaccagt gcatccttat gcctgcaaag
    30301 aacaatcccg ttatcataag gagcatttaa ggtcttatgg gcgtctaccg accaggaatc
    30361 agccaactcc atacccttag tcaggcggtt cattttttcg caggcagctg cccataatcc
    30421 gaaggcaccg tcaatatgca cccatgcgcc ggcagcattg gccttcctgc aaacagaatc
    30481 aaaatcatca aaagcccctg tattgacatt tccggcttgg agtatcaata atgtcctgtt
    30541 gtccggctca ggaatttcat cagctttaat gcgcccctgt ccgtcagtgg gaaccttgat
    30601 aagccttgcg tttcccaagc ctattatgga caacgcctta aataccgttg aatgggcttc
    30661 ttcccctaaa actaccttta tctgaggagc atcgaagagc ccttccttgg tgacatcata
    30721 tcccagattg ccaagcagat aattcctccc tgccagcagg ccgcaaatag tggctgtgga
    30781 actgccgctg acaaagcccg cagctgtatc atcgggtaat tttaaaagct gttttaacca
    30841 gctctcgcat acctcttcaa gctttgaagc aataggagac aatacaaaca gggctgaatt
    30901 ctggtcccaa gcatcagaaa gccactttga gcataatgcc gagggaagaa tgccgccgca
    30961 tacaaatccg aaatacctcc cccccgtctg ggcgactgtt gcaggagaac cgtatttacc
    31021 caacatgttc aatatatttt tggtatcctc cggctcctct gacaaggctt cctcaaacac
    31081 tgacagatta ccgacagcct ttttatcggg gaaaacccgc atgtcattga cctgcttcac
    31141 atatcttaag gccaggtcat gggctaatac catatcctcc tcaaaggttt gctttctgat
    31201 tctgtccaaa atcggactga catagttttt ttctttcata tgaagggtgc ctccttataa
    31261 tttttagtgt atatacacac ttttgccaaa aagttattat tccacaagct gggataatat
    31321 ttccgacaac tgttccagtt tttcctttcc tactttcttc tccagctctg cctgcgccgt
    31381 tctccagagc acttccccct gattaaggac ctgctgcccc gattccgtaa cctttagcag
    31441 acggctgcgc cggccctttt cggaaacgtc ttcgatatat ttcagcttaa aaagcggttt
    31501 cagagagcgt acaagggttg ttcgttccag ccccacatag cctgccagat cactgacgct
    31561 gcaagccttc aacctgccta tatttattaa aagggaatat tggctgacgg taagccctat
    31621 aggcagtaaa accctgttgt ataagtccgt tacggcatta gcagcccgtc ttaagttaat
    31681 gcaatagcaa acgctcttat gccttttaat cataagtata gtattccttc cgcttatagt
    31741 gtatatacac tatattaata tacatttact gttttgtcaa tgtccggtgt tatgggaaat
    31801 ttaaaggagg gtgccccgcc ctccttcagt attgcatatt tatatctgtt atattatgcc
    31861 ttgatttgag tcaaaataga gcatatcagt ttaaatcact tcattgtcaa gtttaccttc
    31921 tacttaacat ttttttgtgt atttcagcat ctgtacaaga tattctcctg aaaggtcatc
    31981 tttttcatcc ctgtcaagct ttcttttacc aaattcaaca aacccattag acgagtagaa
    32041 atttttcaat ttctcattat cttcacattc aagataaaca actttgccac cgagcacaaa
    32101 ttgtgcttca gcaattttat cgcaagccat ctttaatagc tcgtccccgg atatcaaatt
    32161 ctgttcattg tatttgtaat ttttcccaat ttgagctatc aatggcgctg aaaaaacata
    32221 tttctttatc accggattgt atgtgccaaa ccttccgatt tttcttttta catttcctct
    32281 caatacagct ttatcaacaa gaatggtttt gtttgccaaa gtaaaatatc caaccagggt
    32341 aggctcgtcc ttatatgagg taaaaactag aaacgtttgg gaaagagatt gctttgcaaa
    32401 ctcgattgcc ttatggcgca aaaatttttc tacatcagaa ttaagcggac atgaaaaacc
    32461 gagaagaatt tttattgttt catcctcccc gatttcatct aaaatatgtt ccaggtgtac
    32521 gattaaatac cctttaattg tactttcggc actcacttac ggcctccgaa tatatttttt
    32581 acttggcttc tagttgctgt agtgcatgtc ttactaaaat taacttcttt acttgtcttg
    32641 ttctccgcat tttctaaagc cgacgcaaaa gcacgaccca aacgctgatt tttgatgtca
    32701 atgtttttta aaatgctttt tgtagccata tgacatccct cttcctatat cattacaatc
    32761 aattatattg tttactcttg tatttacaac acagtaacat cctatgttca tagtataacc
    32821 caaagtttga atttgttcaa ggtttgacta aattaatcac ttcactacat agtttatcgg
    32881 ttgtattctg caattctaat atatattatg ataaaaaaca atatattcca ctatatttca
    32941 tactgatact ataaaaaaaa gagggctgcc gccctccttc agctatctct tatgctttat
    33001 atgatccata tgctcctgca gtatcattgg gataagggta tacggcatat gaaacgactg
    33061 catcccaatg caaaagtaat ctttgatata acacgtttat tgatatgggt caaaaaataa
    33121 aaagctaacg aaaatcatta gctttttatt tgagggctta tttatacttt ttctattgta
    33181 tatgcagccg tcaatatccc atcaccagct ttcaagaata accccgaagt cactattctt
    33241 ccaaatatct atctaatata aaactattaa gtaagtcctt ttttacacgc caatccggaa
    33301 gatacttatc catatattct acaaaaactg aattgtggct tttttccaat aaatgcacca
    33361 attcatgtac tactacatat tcaaggcatt ctatcggttt cttggcaagc tgcaaattga
    33421 tccaaatgcg tttatctttt atattgcacg ttccccatcg ggtcaacatg ttttttatcc
    33481 tgaaagattc agcctgtata ccaatgatgc cttcccattt tctgaccagt tcgggaagct
    33541 tcttcttaag cagctcacgg taccattctg tcataacctt ctctctttgc tgtgttgtgc
    33601 tggattcccg gacagtcaga atgagcttat tgcctttaat ctctatatta tttctataat
    33661 catgtcctat ttccatccta tatcgtcttc cccagacata gtgactttcg ccggaaacat
    33721 actctcgctt tgtttgccgc tgctggtttt caaatttatc gatttgcctt ttgatccaac
    33781 cgattttggt tatagcaaaa agcttaattg cctcatcttt agtgtttaca ggtgctgata
    33841 tccgtacttt tccgtcaggc ggcaataccg ataaatgcat attcttaatt ttctttttct
    33901 ggatttctat ttcaatattg gagatcacaa ttctatttgc cataatattc tctttgttcc
    33961 tttactatat taaaaatttt atccactgca tcatcatcat tagtgacttc ataaatacca
    34021 cgctgaatta ctttttcttt agccttatct cccagccact tatccggttt tatagagatg
    34081 attttttcat gaagctctaa aataagttct tcatccttag taacattgtc atagaaagca
    34141 cgaagtgccg aagatttttt gattctatca ggatatcctg aatactcttc tggtttttct
    34201 acctttctgg taagctcaac aatcatctcc agatactttt tataatcggc cttttggttt
    34261 ttccgttctt caattatttt cattaacagt tctgacatct tttcgtagta aagaggattc
    34321 gtacttcttt tttcaataat ctttctgcgg acattgttct cgataacctc agcggcagct
    34381 tccttatctt ttctgattcc ttccggcatt tcgtcaacaa aatcgactcc gcgctcaata
    34441 attaattcaa taagg
//
LOCUS       DAOQUR010000023        34011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1485_length_34011_cov_10.173342, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000023 DAOQUR010000000
VERSION     DAOQUR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 34011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34011
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_34011_cov_10.173342"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PKI50_06555"
     CDS             complement(<1..1187)
                     /locus_tag="PKI50_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT03406.1"
                     /translation="MYEIEKGQLTELLTDLVSIESPYLEEDEIMEYVYSWFRKNGIKA
                     FVHHYNEAKVTGFKGKNIVANIEGNKNGPVICLNGHLDTVKLCNGWTHNPKGEIKDDR
                     FYGVGALDMKSGCAATMIAVTAFAENHKDFRGKIIATYVSVEEGPYGMGTNALIDEGY
                     LKDVDFSISTEPSAGFSGNKFPDVCLGARGGYGLDIEFFGISSHAATPELGVSAAEDA
                     STVISELKNIEYIKDPHLGTGNACVIGIECDGGACSVPDYAKVKLFWHIVPGENEETI
                     TGEIEKAIRRASIKCKYKINFRKSPSEGSKGFMPYIVSKEDPMVKEFLETVEEVCKTK
                     PEISYFQSIGDFNYLGSRLGAPVVIFGASGKNFHGKDEYVELDSVVKTAETVYRFLER
                     MLC"
     gene            complement(1217..>2187)
                     /locus_tag="PKI50_06560"
     CDS             complement(1217..>2187)
                     /locus_tag="PKI50_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNT03407.1"
                     /translation="ISGAVIGDKISPLSDSTNLAAGVTETDLFAHIKSMFYTTVPAFV
                     VSLILYGIIGARYSASSIDTSVVEQILAGISQNINITPMYALISLIPLIAIVVMAVKK
                     VNALAAMVIASIIAMVLAMINQGYSIIEMMAFMNYGFSIDTGVAEVNKLLNRGGIQSM
                     MWTVSLGYLGLSYGGILEKTGIMEVLLNKMSALVKNARNLIITHVFAALIVNLISASQ
                     YVSIIIPGRMLLPAYRKLGIRSDVASRTCEDSGTVLSPLVPWGLCGVFFTGTLGVNTL
                     EYMPFAFLCYLTPIVAIIYAITGKFIWYEKDGMIKNDVKKAAEISG"
     assembly_gap    2188..2287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2288..3381)
                     /locus_tag="PKI50_06565"
     CDS             complement(<2288..3381)
                     /locus_tag="PKI50_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNT03408.1"
                     /translation="MSNTPKQIKSINVFSKGTIAVVIIITVAGLSILGVFRFTRPIPA
                     AGTSKTSENIVLPGELSVAFPEQGQAAVGTETFGVIAASPDQEPIPIASVAKIMTAYL
                     VLKAYPLQPGEEGPSLTITDEDVAGYKYAVQNNHSYLPVAAGTALTERQLLQGLMLPS
                     GNNIADTIGRWIAGTNEAFIDKMNETAKALGMTNTHYADASGADDATVSNAADQIIMA
                     QAAMQDPAFREIVAMPQAVLPVAGKVYNVNAMLGKHGIVGIKTGSGVIAGGNFVSAAP
                     IENGSEKHYIIAAVLGSRKPNENLKSAFDANAQILNQVRPQFKTFTIEPPENGFGKII
                     TPWHTESELTVNEPIQVFGYPGMKMDYSIEP"
     assembly_gap    3801..3900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3901..4379)
                     /locus_tag="PKI50_06570"
     CDS             complement(<3901..4379)
                     /locus_tag="PKI50_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid-binding protein"
                     /protein_id="HNT03409.1"
                     /translation="MKKLLILLLVLVMALVPAACAKQAPPAQQPAEQPAAEEPAEGEN
                     KYASLEPITLIGADNAGVGAAAQLYGELVTKKVAEITDGKLKIDYFPNSQLGNDQELQ
                     AQMLAGDIDFVIAQTAQTVSFVPEVAIFDLPMVFAKYDAKTIDYALNKSPFAAKINEA
                     "
     gene            complement(4426..5709)
                     /locus_tag="PKI50_06575"
     CDS             complement(4426..5709)
                     /locus_tag="PKI50_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNT03410.1"
                     /translation="MALWIFVVFLSALVFAVPIAVSMVIGAMTPLFLGGTGSSIQQLI
                     ANSFSGSDTTPILAVPLFILGGVLMAEGGISRRLFNFFAYFVGNLTGGLPCAVILTCL
                     FYGAISGSGPATTAAVGSMTIPFLVDMGYDKTWSAGMVATAGGLGVIIPPSIPFVLYS
                     LATGVSTGALFLGGVLPGILIGLCMMVYAVIYCKRKGENKERINAKMTELRSKGFLSL
                     LKESFWALLSPVIVLGGIYSGIVTPTEAACISVFYSLFVSMVIYKSFTVKEIIPFLGN
                     AVKTYAPLCFVLAFAIAFGRVLALAKAPILIENFILNNFSTSITALTGIVVVFLLLGM
                     VMDTGPAIVILSPILLPAVQALGVDPVHFGVIMVCCLSIGLATPPFGLDLFVAGTLSS
                     LPPMKVAMKAMPFIIAFGIALFIITYIPWFSLVFL"
     gene            complement(5709..6287)
                     /locus_tag="PKI50_06580"
     CDS             complement(5709..6287)
                     /locus_tag="PKI50_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT03411.1"
                     /translation="MNGLKWLNKHLEEAILVFLLATISCVMMAQIIARTFFNSMTWPE
                     EFSRYCYIWTVFLSLGYTIKKGNMLKVGIVMDLLPQKLRKRIEIIVNVIMLVLFVILL
                     RYSIIYTGKVKSSGQFSPAMHLPMWIMYMSTIIGFALAALRMVQEIISNIRNFNKKAE
                     TTLEATIKEAKQEVQATGIAVNDNDGLMGGED"
     gene            complement(6338..7216)
                     /gene="aroE"
                     /locus_tag="PKI50_06585"
     CDS             complement(6338..7216)
                     /gene="aroE"
                     /locus_tag="PKI50_06585"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNT03412.1"
                     /translation="MPVNINTKMITLLGKPLSQSYAARMQNAAYRAAGIDMLYFYTEV
                     ENDHLPDVINGIRHMNFAGFAVTKPNKVEVMKYVDEVDPLCEKMNASNTVVIKNGRLK
                     AYNTDGIGFLRSLKEEAPEIDISESVFFCLGAGGAARAICSVLAYNGAKKIYIASRTL
                     SRVKELVEDINEKFAPVAEQVDMQDTELLKKSIAASNVIMNNTGLGMPASLDKTPIPK
                     EYLSPVQLCFDATYNPSKTRFLTEAEQVGCKIINGLGMSLYQGAEQIELWSGVNAPIE
                     AMRQELLDILSGKEEK"
     gene            complement(7269..8090)
                     /locus_tag="PKI50_06590"
     CDS             complement(7269..8090)
                     /locus_tag="PKI50_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT03413.1"
                     /translation="MPRKFSLAYLTIPGTGPVDQIRIAAEAGYDFVSLRPIPMHLPNE
                     PLFRFDQDKKLFNEIKHALSENNIRLLDIELARVREDLKVEDYESAFFAAAELGGTDV
                     LSSIWTKDKAYYYREFAKICDLAAKYSLRVNLEFVTFAGVTSLVEASDVLNTVERPNA
                     YLMVDTLHAHRSRVSAEDIAKVEPSKFGFIHLCDGPGPIPALDDPGMIGVAREGRLYP
                     GDGEIDLKSMFLAMPANPISIELPNSKEMEKRGAFGHAAQCLIKAKQYFAENGIE"
     gene            complement(8117..9910)
                     /locus_tag="PKI50_06595"
     CDS             complement(8117..9910)
                     /locus_tag="PKI50_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNT03414.1"
                     /translation="MVNYLQRRGVEYVFGLCGHTVIAFLDALSRSSSVKYVSFRNEQL
                     AAMAADGYARIKKKAGVVMCHLGPGIANTVTGVANASFDSIPMVVIAGDVPRYYFGKH
                     PHQEVNLHADAAQYEILKPIVKRAWRVDDPEAMPEILDKAFRMAESGRPGPVLISVPM
                     DVFSEEVEEDLFARTYLDRHQTIKPALAPKAAKAIAQKLVEAKNPVIHAGGGILLAGA
                     SKELQDLVEFLDIPVSRTLMGQGCMSDTHPLMLGQTGFWGLEFTHKFTANADVLLGLG
                     TRFAEADSSSWYPGVTFDMRKTEFLQIDIDPTEIGRNYPVSIGAAADLKEALAQILEE
                     AEKLCPEGIKRPELREMIKKNRTLFRESNIAISEDGRFPMTPQRILKDVKETIPENTI
                     IFTDVGWNKNGVAQQFDITLPGTIHHSSGLATMGFGASALLGGKLAAPDKVVITLVGD
                     GGFGINPSSLATAVEQGIAVIWIVMNNSAFGTIAGLEYSNYKTKFGTVFTTPDGKPYS
                     PNWAEVAKAYGVDAIRIDSAEAFKPALEQALASNRPFLLDVPMENIGVPTPGCWNIND
                     IYTPKANIVNGKIIKNEKGEYEAPNHSGSHK"
     gene            complement(9949..11421)
                     /locus_tag="PKI50_06600"
     CDS             complement(9949..11421)
                     /locus_tag="PKI50_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005903036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT03415.1"
                     /translation="MSRMSSMEEVYSKLNQDQIDEIEEKFARADAAQKIFEKWSQEEV
                     DRTIRAIAWKVANTQTFKELVKMSIEESGMGDPVSRENKKFKIRGVLRDALRQKSVGI
                     IEEIPEKGIVKYGKPVGVIACVVPATNPDLTPAGNAIYALKARNAIIFSPHPRAVKTG
                     GRTIELMREGLRQVGAPEDLIQILGVGKAKINKAMSEALNTKCDLVIATGGQGVVRRA
                     YHSGTPAFGVGAGNATMIWDETAKDELHQAAYNTMLSKTSDFGSGCSADGNIVIHESI
                     YDDAIKELVNVGGYLLSEEQQLAVKKIMWDEECHRLPDSVAQSPQRMAELAGFTIPED
                     RKFLIAIGTGSYTTSYEDVWCREKLTTLLAVHKYEGEFQNAIDIIMAIHEVGGKGHSV
                     GIYSYDEEHIHRLALEARVGRIMVRQPQSKSNAGSFTNGMPMTSSIGCGTWGGNATSE
                     NVNLRHYMNVTWLSKAIPEDKPSDEELFGEFYEPEREGRV"
     assembly_gap    11445..11544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11545..12278)
                     /locus_tag="PKI50_06605"
     CDS             complement(<11545..12278)
                     /locus_tag="PKI50_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNT03416.1"
                     /translation="MNLPSKITICEVGLRDGLQNEKKILQTQEKLELANGLIAAGFKV
                     IELGSFMNPKAVPQMADTDELFSTLNQIDGVELRALIANKKGVERAINCGCRKVKLNV
                     SASLGHNLANLNRTPEESVAGFEESVALAEQHGIKVSGSISMPFGSPWEGNISLESVK
                     SIVEAYLNVGIDEISLSDASGMAVPNQVYSMCINMQECYPQVKWWLHFHNTRGMAMAN
                     ILAAMQAGITQFDSSFAGLGGCPFVPG"
     gene            12745..13653
                     /locus_tag="PKI50_06610"
     CDS             12745..13653
                     /locus_tag="PKI50_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT03417.1"
                     /translation="MTLEQLRYFVETAHTLHYTKAAEQLNISQPSLSYAISQLCKRLG
                     VPLFKKEGIKVSLTEYGEAFLPYAESALNILIQGEAQLNRMNSPYGGNINLGYIYSVS
                     FDEIPHLIDDFYACQGNRNISFSFQVNMTNLLIKKLIEGTLDVVMAPSTEIVNECIEF
                     IPLFEQELFLVVYKDHPLASHTSVTIDDFKNEKFIMIKKETDLHFQTDSLFKKFNIVP
                     NIFFEVDECNSMASFVGAQLGIAIMPKIPSLDGYKVTAIPFEGRTVRRTISLLWNNRI
                     KHTPALKSFLDYCISSRSTGIKNTEK"
     gene            complement(13940..14557)
                     /locus_tag="PKI50_06615"
     CDS             complement(13940..14557)
                     /locus_tag="PKI50_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT03418.1"
                     /translation="MAISERREREIEEMKELILSTAAMIANSEGYDKLSIRKIANKIE
                     YSPSIIYHYFADKDEIISHLMQRGYKKIISAIASANMISDSPEEQLKKMARYYIDAAL
                     KMPEEYMAAQLSKSPKTIKQTSFLYEGASDEKSVIGALCKCIKEINKDIDLEDSKCEL
                     ISQLIIASAMGLVIKLIAEKETGEEQRNRLVECFANEVVPKIARV"
     gene            complement(14723..15613)
                     /locus_tag="PKI50_06620"
     CDS             complement(14723..15613)
                     /locus_tag="PKI50_06620"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HNT03419.1"
                     /translation="MEFNQIKIFVAVARKKSFSKAADMLFISQPAVTSNVQKLESELG
                     VTLINRKTKNISLTEGGKLFYRYAVELINIYEKAEYALSGYKKNIEGTLELYASTIPG
                     QYLLPYIVKEFKKAYPLVLFSICHKDSSEVMEEILSGRINLGLVGAKYSSDALEYIDF
                     YDDRMVLIASPQKKFDSDSVSIRSLIGENILLREEGSGTRLLLENALKEKELDMSMFK
                     SQIINDSLEAIKKMVELDVGISLVSDIAVKREVESGQLKQYEISDLELKRSFSLVYCN
                     NRCLSPIEEKFKDFISNWKR"
     gene            complement(15629..16774)
                     /locus_tag="PKI50_06625"
     CDS             complement(15629..16774)
                     /locus_tag="PKI50_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT03420.1"
                     /translation="MKEIYMDNGATSFPKAPGVAESVYKYLIHVGANINRGSYSNSFQ
                     AENVVFETRELLCSLFGFNKPENVVFTKNITESLNVLVKGLFKSGDHVIVSSMEHNAV
                     MRPLRALEGKGVTFSRVLCKNDGSIDGKDLLPLILPNTKGVVMTHASNVCGTILNLEE
                     VGSICKEKGLRFIVDTAQTAGAIKVDFDDMCADAVAFTGHKGLLGPQGIGGFLVREGL
                     ADELSAFIEGGTGSLSDMEIQPSYMPDKFEAGTPNVPGIYGLNASLKYIQETGIQDIY
                     EKEMRLMGVFLERLMNMPHIDIIGKKDTKGRTGIVSVDFKTQDNAEMAHRLSSESGIM
                     TRSGLHCAPSAHKTLGTFPRGTVRFGLSHFMEEADILYAADQIYKLK"
     gene            16965..18029
                     /gene="yedE"
                     /locus_tag="PKI50_06630"
     CDS             16965..18029
                     /gene="yedE"
                     /locus_tag="PKI50_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HNT03421.1"
                     /translation="MKEKTGIIITGGVVGLFAVLLVRFGNPANMGFCIACFLRDIAGA
                     LGLHRAEVVQYIRPEIIGLVLGSFILASMNKEFNSQGGSSPVTRFVLSIIVMIGALIF
                     LGCPLRMVLRIAGGDFNAIIGLIGFVSGIYVGIFFLNKGFNLKRSYKLTNLEGYLFPA
                     INVGFLALLIVAPAFVFFSKSGPGAATAPIWISLAAGLIVGALAQKYRLCTVGGTRDL
                     ILFRDNYLMLGFLSILAVALVGNLILGKFNPGFAEQPIAHTDGLWNFLGMALVGWGSV
                     LLGGCPLRQLILAGEGNTDSAISALGLIVGAAICHNFGLASSAKGVTLNGQISVGICF
                     LILLAISYANSEFLSKNAQS"
     gene            18042..18248
                     /locus_tag="PKI50_06635"
     CDS             18042..18248
                     /locus_tag="PKI50_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNT03422.1"
                     /translation="MKTIDTCGMSCPQPVLMTKKALDEEKDGIDVIVDNNTAKGNVQR
                     YLKSVGYSVEIEENDGTFVLKARK"
     gene            18245..18490
                     /locus_tag="PKI50_06640"
     CDS             18245..18490
                     /locus_tag="PKI50_06640"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNT03423.1"
                     /translation="MNKEYQVLFYTHTGAIKFERAMKKENISCELMPVPRSLSSNCGV
                     SARIYYNGQVDQLINDQVEKIYQKYDGNYILTYEAEK"
     gene            complement(18560..19399)
                     /locus_tag="PKI50_06645"
     CDS             complement(18560..19399)
                     /locus_tag="PKI50_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNT03424.1"
                     /translation="MERKVEQQVRGFRTQDGAGVNLVRVLGKGTASEYDPILMLDSFD
                     STNPDDYTAGFPMHPHRGIETISYVYRGYMTHKDSLGNEDTIGDGEVQWMTAGSGILH
                     EEKLPASERLLGVQLWLNLPAKDKMVPPAYRSIKNAEIEEVKLENGKLRLLAGEYEGR
                     KGYMSKYLPLDYYDLHLNADTSVSVKTERSRSVMIFTLLGDAYIGGESVKEKTAVKLT
                     SGEHVEIIATNKNAQLLFVSSTILAEPIAWGGPIVMNTKEELNKAFDDLKNGTFLQKK
                     ISY"
     gene            complement(19484..20509)
                     /locus_tag="PKI50_06650"
     CDS             complement(19484..20509)
                     /locus_tag="PKI50_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNT03425.1"
                     /translation="MMNFSHYITCIDAHTAGEPLRIITAGFPPIPGNTILEKRDYVNK
                     NYDGLRRMLMLEPRGHSGMYGCILVPAVTPDGDFGVLFTHNEGLSSMCGHGIIGVTKV
                     VIETGMIGCTEGENVIRIDSPAGRVTAYADVEKGKVLRVRFHNVPCFVYKENITVHVD
                     SLNRDVVCDIAYCGAFYVYIDADRVDLEVEPQYAEQLVKIGMEIKHKVMEEYTFKHPT
                     EPGIDWLYGTILMSPVERIGNRLISNNICIFAEGQIDRSPTGTGTGGRTALLVHKNIM
                     QEDDTLVNKSVIDTPMESKVISRCKVGQYDAVITEVSGTAHITGFNNIVLDPDDPLPE
                     GFRIIGS"
     gene            complement(20547..20900)
                     /locus_tag="PKI50_06655"
     CDS             complement(20547..20900)
                     /locus_tag="PKI50_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03426.1"
                     /translation="MLPDDAELLEGKHFIGIGSYKPNMREYSESIYKLLEKIYIDVDF
                     AREESGDLATPCEKGWFKEENIRTLYSVVKADSIDRSRTTFCKSVGMALFDIFVAGNI
                     YNKAIEQGIGQKLNP"
     gene            complement(21220..22104)
                     /locus_tag="PKI50_06660"
     CDS             complement(21220..22104)
                     /locus_tag="PKI50_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT03427.1"
                     /translation="MSTAPLSKERSRAMLLLAATAAMWSIGGLLIKIVDASPLSIAGA
                     RSAITSIVLLIYLRRPKFNWSLPQVGAAISSSATAILFITATKTTTAANAILLQSTAP
                     IYVAIFGFWLLKEKTTKYDWLTVAVVMGGMGLFFVENLSTEGLLGNFCALLSGISYAF
                     FVIFMRMQKDGSPLESALLANIITALIGLPFLCRSVPSTSGWLFLLILGVFQLGIPHI
                     LYSSAIKKATALEAVLISIVEPILNPVLVFLVLGEAPGIYALLGGAIVLVSITLKCVL
                     TIIPLKIVSEDKKEQPLE"
     gene            complement(22152..22781)
                     /locus_tag="PKI50_06665"
     CDS             complement(22152..22781)
                     /locus_tag="PKI50_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HNT03428.1"
                     /translation="MAEQKASSKKVTLWTRQDIRSLEDLKKEGVLRIKRKHLVEKFDL
                     IADYIINLYSWFVNAADRMVPKPEGVEFPIWCSISEEYMLRPTDTEIVYVLEVDESEI
                     IYFDGCKWDYVLNHHYVPKDDKDLEEYERDISDKGFRDSYSFLDENTSHFYPEERKRV
                     MDSWIRVFETDQWDPFRIQANMWEIRPEMIKGILKKDVTIDAMLSQMIK"
     gene            complement(22884..23432)
                     /locus_tag="PKI50_06670"
     CDS             complement(22884..23432)
                     /locus_tag="PKI50_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNT03429.1"
                     /translation="MVHRFESHTPSLDTKSFVAEGVQLIGRVVMKEYSSIWFNSVARG
                     DVNAIEIGCYSNIQDNSVIHGTDDCASIIGDYVTVGHGCIIHGATIEEHCLLGMNSVI
                     LAGSVIGKGSIVAAGSVILENEIIPPHSLVVGVPGKVIKQIPGSWEQIHAQAIKYKTL
                     WTERYGLLPNAGGERYNGEKIV"
     gene            23748..24842
                     /locus_tag="PKI50_06675"
     CDS             23748..24842
                     /locus_tag="PKI50_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNT03430.1"
                     /translation="MQLEKQLEKIVGLMSDINADIHMIGPSSGLEYLTGINPHPDERF
                     KALFILKDKRHFYISPEIYYEETRELLGEKEDIFVWSDTDGFLSAIEKADKKYGLAGK
                     TIAVNDGIVAINMLDIGNFLDVKFINGNSILEDVRIRKTQEEKDCMRKAAKIADEVVE
                     ETIKFIKPGLTERDIAKKLEELYTEKGASMSFHPIVASGPNTSKPHYNGDSRVIQEQD
                     IIIIDTGCRYKGYCSDISRTIFVGEPTEEQRKIFDICVKATTAGEMAVREGITAEEVD
                     MAARNIIKAAGYGEYFLNRTGHGIGIAVHEGPYIRTGNKQILENGMCFSVEPGIYISG
                     NFGMRVENIVLVENGKAEILNKVTKDIIIV"
     gene            complement(25043..25447)
                     /locus_tag="PKI50_06680"
     CDS             complement(25043..25447)
                     /locus_tag="PKI50_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03431.1"
                     /translation="MDHIFKTIEKGVDTAGVMKRTPVPETSFTDEITNARLAQIASVN
                     ALAMTAIAPYPWIPVHEADYISLVSGANSICAETGVNPRDTEADTSKGRGLDVTACRK
                     MLWQAGFKHLAKGDGTRIELTPEYITKCETTC"
     gene            complement(25618..26115)
                     /locus_tag="PKI50_06685"
     CDS             complement(25618..26115)
                     /locus_tag="PKI50_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03432.1"
                     /translation="MQKTYRFMKDTNNIRRIKYRSENNSFFDEKVSYIIDSLDDAHKL
                     SQRSFLQRVFEATFKLIPEAEKGSFYELNDDKYTPIFSMNYDFELLKKLSFNKDDAFI
                     DFECDDTSSIDAYQVYISKRDDSKFSKEIKNYSQQSRSVYLLRQNRLCCIRISVIPIT
                     RRLSN"
     gene            26307..26516
                     /locus_tag="PKI50_06690"
                     /pseudo
     CDS             26307..26516
                     /locus_tag="PKI50_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(26665..27948)
                     /gene="kynU"
                     /locus_tag="PKI50_06695"
     CDS             complement(26665..27948)
                     /gene="kynU"
                     /locus_tag="PKI50_06695"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNT03433.1"
                     /translation="MKHEFRLGLDCAKELDMQDPLKEVRNRFYVRKDEIYMDGNSLGL
                     CSKDAEKAVLKVMDVWKEHGIGLWNVEDSKYFLYPSYLGGRMASLINAEANEVTVTNS
                     VTINIHQGIATFYKPTKDKYKILVDDLNFPTDRYAVDSQVKLHGYDPADVVKVVQSPD
                     GRIISEDAVIEAMTDDVALILLPSLLYRSAQLLDMERITKEAHKRGIYIGWDLCHSIG
                     VIPHDFKKINPDFAVWCNYKYMSGGPGTSAGLYINKKHFAMDPGLTGWFGNTKSTQFQ
                     LKHQYEHSNDADGWLTGTPPILSMAAIEGVLDIYDEIGIVRIREKSLKITDYLMYLID
                     ERLTKYGYSIGNPREDDKRGGHVALEHQEAYRICQALKRHKVIPDFREPDVIRLAPVA
                     LYVSYEDVHRLVDILEELTISKEYEDFEHTRSLVV"
     gene            28875..30027
                     /locus_tag="PKI50_06700"
     CDS             join(28875..29141,29143..30027)
                     /locus_tag="PKI50_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458102.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNT03434.1"
                     /translation="MEKRRNFTPEEKAKIVIEVLREEKTLNEIAAQYEVHPNQLSRWK
                     AEFISNAARVFSKETDEAEKAKQSYEKEKDELLKQIGQLSYEVAWLKKNLAGSKSRDD
                     RMKMIDKGDEKLSITTQAGFLSINRTSLYYKPVPISDEEYLIKRIIDVVYTAHPEYGY
                     RRMTVILNRDYGILINRKRVRRYMREMGIHGFCPGPNLSKRLHKKYLYPYLLRGLNIN
                     HPNRVWPVDVTYCRMKHGFMYMVAIIDWYSRYIVGYSLSNTLERTFIIEAVKNAIKQH
                     GAPEIINSDKGSQFTSEDYINLLKDNNIKISMDGKGRALDNQRIERFFRNYKWEKLYL
                     EDCETGHQLRKITQEYMEHYNYKRPHQSLDDITPAEFYFGILNQYPKAV"
     gene            30722..33838
                     /locus_tag="PKI50_06705"
     CDS             30722..33838
                     /locus_tag="PKI50_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT03435.1"
                     /translation="MQPTMIALLNNAALLLLLPVIYQLTSLINTRNTRFKQIVSGILI
                     ALLCIAIMSVPFKFYLGVQFDTRSILISVTALVFGPIPTIITIAAAIILRILQGGAGA
                     LPGVAVIISSAIIGLVWRLWIHSAATKLRWLNVYAMSLLVHAAMIACMNLLPYPASVN
                     VIREITLPVLLIYPAATVPIYLLLTQQQVFLQVRDQLKQSEERFRLLFDKAPLGYESL
                     DIDGNIIDVNQQWLNTSGFSRDEVIGKWFGDFIHPAYKEEFLEKFSQLEETKQIHSEF
                     EMLHKSGKSLFIAVDGNIVYEPNGDFNETFCILKDITRQKKVETDLQSSEKKYRRLYE
                     TMAQGVIYQAADGRIISANPAAEKILGIPLDQMLGMSSLDPRWRTIREDGSELSGMDH
                     PSMIALRTGKPYGPVILGVYQPMLKDYVWISVNAIPLFEDGEAAPSKVYTTFQDITAE
                     LKANQNYQLLFNEMVDAFALHEIICDDQGTPIDYRFLAVNPAFEKMTGLKAADILGKN
                     VLEILPNTESYWIETYGKVALTGESVSFENYAAATDKYFRVMAYQPAPMQFACTFSDD
                     TTQVRAEHALKESEKKYSSYIENAPYAVFVVDKKGQYVEVNNAASIITGYSREQLLEM
                     TIRDITAEESMNEAMRCFTSMLDTGKLSTELKYLHKDGSIRWWTVDAVKISENRFLGF
                     SIDITSKKQAEEELTFQSRHDYLTGLYNRRYYETELKRLDIEGQLPLSVITGDINGLK
                     LINDAFGHAEGDKIIIESAMIIQGCCRQGDILARTGGDEFGIIMPRTDSVTALEVLQN
                     IQTALIKFDSENNFEMYQHSVSLGYATKEKIEEDIMYILKTAEDHMYQRKLLEANSVH
                     STIVASIKAALFEKSHETEEHAERLVELSKAIGMALNLSQVELDKLELLATLHDIGKV
                     GIKDNILTKPGKLSESEWAEIKRHPEIGYRIAMTSPELVPIAEGILCHQEWWNGSGYP
                     KGLCGESIPLLSRIIAVVDAYDAMTNDRPYRSAMEPAAAIEIIKENAGTQFDPKIAEI
                     FADLMSKE"
BASE COUNT         9567 a         7621 c         6491 g        10032 t
ORIGIN      
        1 aaacacagca tcctttcaag aaatctatag acagtttcag cagtttttac tacggaatct
       61 aattctacat attcatcttt cccgtgaaag ttttttcctg aagcgccgaa gataacaacc
      121 ggagcgccta atcttgaacc taaataatta aaatctccga tgctttggaa ataagaaatc
      181 tcaggcttgg ttttacatac ttcttcaaca gtttccaaaa attctttaac catgggatct
      241 tcttttgaaa ctatataagg cataaaacct ttagaccctt ccgaaggtga ctttctaaaa
      301 ttaattttgt atttgcattt aatactagct cttcttattg ctttttcaat ttctccggtt
      361 attgtctctt cattttctcc cggaacaatg tgccaaaaca gcttcacttt agcataatca
      421 ggaacactgc atgcacctcc gtcgcactca atacctataa cacaagcatt gcctgttccg
      481 agatggggat ctttaatgta ctcaatattt tttaattcgc tgattaccgt tgaagcgtcc
      541 tccgccgcgc ttactcccag ttcaggagtc gctgcatgag aagatatacc gaagaattct
      601 atatccaaac cgtatccacc tctagcacct aaacaaacat cgggaaattt gttgcctgaa
      661 aacccggcac ttggttccgt gctgattgaa aaatcaacat cctttaaata tccttcatca
      721 atcaaagcgt ttgttcccat cccatagggt ccctcttcta cggaaacata cgtggcaatt
      781 atcttacctc taaaatcctt gtgattttca gcaaaagctg ttactgcaat cattgttgct
      841 gcgcatccgg atttcatatc taaagctcct actccataga acctatcatc ttttatttcc
      901 cctttggggt tatgagtcca tccgttacac aacttaacgg tatctaaatg accattcaaa
      961 caaattaccg gtccattctt attaccttca atatttgcaa caatattctt tcccttaaag
     1021 cctgtaactt ttgcttcatt ataatgatga acaaaggcct ttataccatt cttccggaac
     1081 caactgtata catactccat tatctcatct tcttctaaat acgggctttc aatagaaacc
     1141 aaatctgtta ataattctgt taattggcct ttttcgattt catacatctt tattcccttt
     1201 ccaaagccga ttttcattat ccactaattt ccgcagcctt ttttacatca ttctttatca
     1261 ttccatcctt ttcataccaa ataaattttc ctgtaattgc gtatataatc gcgacaatgg
     1321 gtgtcagata acataaaaat gcgaaaggca tatattccaa tgtattaaca cccaaagttc
     1381 ctgtgaagaa cacaccacat agcccccagg gtaccaaagg cgataataca gtccccgagt
     1441 cttcacaagt tctggaagca acatcgcttc ttataccaag ttttctgtag gcaggcaaaa
     1501 gcattctccc aggaataata attgatacat attggctggc tgaaattaag ttgacaatga
     1561 gtgctgcaaa gacatgagta ataataaggt ttcttgcatt ctttactaat gcagacatct
     1621 tgtttaacaa tacttccatt atacctgttt tttcaaggat tcctccgtag gaaagcccta
     1681 agtaccccaa agaaacagtc cacatcatag actgaattcc gcctctgttt agaagcttgt
     1741 tcacttccgc aacccctgtg tcaatactga agccataatt cataaatgcc atcatttcaa
     1801 taatgctata accttgatta atcattgcta ataccatagc tattattgaa gcaatgacca
     1861 tagctgcaag agcatttact ttcttaaccg ccatcacaac aatagctata agcggaatta
     1921 aactgatcaa cgcatacatt ggggttatat tgatattttg tgaaatcccg gcaagtattt
     1981 gttctactac ggatgtatca atggaagatg cactatatct cgcaccgatt atgccatata
     2041 gtattaatga aacaacgaat gctgggactg tggtataaaa catacttttt atatgggcaa
     2101 aaagatctgt ttctgtaacc cccgcagcta aatttgtaga atcagacaag ggagatatct
     2161 tatctccgat aacagctccg gaaattannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnggt tctattgaat aatccatctt catacccgga tagccaaaga cctgtatcgg
     2341 ctcgtttacc gtcaattcgc tttcggtatg ccatggagtg ataattttgc caaaaccatt
     2401 ttccggaggc tcaattgtaa atgttttgaa ctggggccga acctggttca gaatctgggc
     2461 gtttgcatca aaggcgcttt tcaggttttc atttggcttc ctgctgccca gtacggcagc
     2521 tataatataa tgcttttcac ttccgttttc tatcggtgcg gctgatacga agttcccgcc
     2581 tgctataaca ccggaaccgg ttttgattcc cacaatgccg tgtttgccta acatggcatt
     2641 tacattatat acttttccgg caacaggaag gacagcctgg ggcattgcga ctatttcccg
     2701 aaaggccgga tcctgcatag ctgcctgcgc cattattatt tgatcggcag cattgctcac
     2761 tgtagcatca tctgccccgc tggcgtcggc atagtgtgta ttggtcatcc ccagagcctt
     2821 tgctgtttca ttcattttat caataaatgc ttcattggtt cccgcaatcc atcgtcctat
     2881 tgtatcggct atattgttcc cggagggcag catcaaacct tgcagaagct gtctttccgt
     2941 aagagccgtc cctgcagcaa cgggtaaata agaatgattg ttttgaaccg catacttata
     3001 accggcaaca tcctcatcag ttatcgtcaa gcttggccct tcctcaccgg gctgcagagg
     3061 gtaagctttc agaaccaaat atgcagtcat tatttttgcc acacttgcaa ttggaatggg
     3121 ctcctgatcc ggcgaagcag caataacacc aaaagtttca gttccaacag cggcttgccc
     3181 ttgttcagga aaggcaaccg acagctcacc gggtaaaaca atgttttctg aagtttttga
     3241 ggtgcctgct gccggaatcg gcctggtaaa gcgaaaaact cccaatatgc tcaatccagc
     3301 aactgttata attataacaa cagcgatagt cccttttgaa aaaacgttta tagattttat
     3361 ttgctttggt gtatttgaca taaaaacctc cgactattat tatttaaaat gctatatcat
     3421 attttctttc ctgactgcat tttttcctgc ctgaatttac tggtttttat tggaatactg
     3481 ggatattaca gcagcacttg cagaaatgtg ttattgagtg caggaacctc attttcttag
     3541 ccttgcagca tgcaaaacag aggtcgattc tctcgacctc tgttttaact gtttaagttt
     3601 ttctaccctg tttgatttcg aaaaagctgc atttcgattt tcaattacgg gttatttcgc
     3661 gttctcaagt gcatccagca gagagtcaac ataatcctgt ccgatggctg ctccgataga
     3721 tgaatatacg cctttagcct tctcaagaac ctgatctatg aagctggctt caaattcaac
     3781 cagttccatt ccgccgtcta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 gcttcattaa tctttgcggc aaaaggactc ttgttgagtg catagtcaat agtcttggca
     3961 tcatacttcg caaacaccat tggaaggtca aatatagcaa cttccggtac gaaggacacc
     4021 gtttgggctg tctgtgctat tacaaagtca atatctcctg caagcatctg agcttgaagt
     4081 tcctggtcat taccaagctg gctgttcggg aaataatcga ttttcagctt tccgtcggta
     4141 atctctgcta cctttttggt aaccagctca ccgtaaagct gtgctgcggc gccgacacct
     4201 gcattatcag caccgataag ggttattggt tccaaagaag cgtatttgtt ttcgccttct
     4261 gcaggctcct ctgctgccgg ttgttctgcc ggttgttgtg ccggcggtgc ttgtttagca
     4321 catgctgccg ggactaatgc cataaccagt accagtaata agataagtaa ctttttcatt
     4381 tttttccccc ttttcttgtt ttcatttata acttgaatac ctgttttaca ggaatacaag
     4441 gctaaaccag ggtatatatg ttataataaa cagcgctatg ccaaatgcaa ttataaaggg
     4501 cattgctttc attgccactt tcatcggagg taaactggac agtgttccgg caacgaaaag
     4561 atcgagtccg aagggtggag ttgctaatcc gatcgacaga cagcagacca ttataacacc
     4621 gaaatgtacc ggatctactc ccagggcctg aacggcagga agcagaatgg gggacagtat
     4681 tactatggcc ggtcctgtat ccattaccat tcccagcagc aggaaaacca ctacaatacc
     4741 cgtcaatgct gtaatacttg ttgagaagtt gttcagtatg aagttctcaa tcaatattgg
     4801 agccttagct aaggccaata cccggccgaa ggctattgca aaggcaagta caaaacaaag
     4861 cggtgcatag gtcttcacag cattccccag aaaggggatg atttccttaa ctgtaaaaga
     4921 cttatagata accatagaga cgaatagaga ataaaataca gaaatacatg ccgcttctgt
     4981 cggtgttact attcctgaat agatacctcc taatacgatt acaggtgaca gcaaagccca
     5041 gaagctctcc ttcagtaaac tgaggaaacc ttttgagcgc agctctgtca tctttgcatt
     5101 tattctctct ttgttttctc cctttcgttt acagtagata acggcataaa ccatcataca
     5161 gagtccgata aggatacccg gaaggactcc acccaggaag agagctccgg ttgatactcc
     5221 tgttgccagt gaatacagaa caaagggtat ggatggaggt atgataactc caaggccgcc
     5281 tgcagtagca accatgcccg ctgaccaagt cttgtcatag cccatatcca ccaggaatgg
     5341 aattgtcatc gatccgactg ctgcagtagt ggcagggcct gatcctgaaa tagctccata
     5401 aaagagacaa gtaagaataa cagcgcatgg caatccgccg gttaaattac ctacaaaata
     5461 tgcaaagaaa ttgaagagcc ttcgggatat cccgccttct gccataagta ctcctccaag
     5521 aatgaagaga ggtactgcca ggattggtgt cgtatccgaa ccggaaaagc tgtttgcaat
     5581 cagctgctgg atggaacttc cggtccctcc taaaaaaagc ggtgtcatag ctcctatcac
     5641 catacttaca gctatcggaa ctgcaaatac aagtgctgac agaaatacaa cgaatatcca
     5701 tagtgccatt aatcctcacc tcccattaaa ccgtcattat cattaacagc tatgccagtg
     5761 gcctgaactt cctgcttcgc ttcctttatt gtcgcttcca aagtcgtctc agctttcttg
     5821 ttgaagttcc tgatatttga aatgatttcc tgtaccatac gtaaggcagc taatgcgaaa
     5881 ccgataattg ttgacatgta cataatccac atcggcaggt gcatagccgg tgaaaattgt
     5941 ccgctgctct tgactttgcc ggtatatatt attgaatacc tgagcaatat tacaaataat
     6001 accaacatta taacgtttac aataatctct attctctttc gcagcttctg gggaagcaag
     6061 tccataacta ttcctacctt cagcatattc cccttcttga ttgtatatcc gagagaaaga
     6121 aatactgtcc atatgtagca atatctggaa aattcttcag gccaggtcat ggaattaaaa
     6181 aaggttcttg caatgatttg cgccatcatt acacatgata tggttgctaa cagaaacacc
     6241 agaatagctt cttctaaatg cttgttcaac catttaagtc cattcatatt gttgcccccc
     6301 ttcccattgt gtaagtataa tacatacgtt acttacacta tttctcctct ttccctgaaa
     6361 gtatatcaag gagttcctgg cgcattgctt ctataggagc atttactccg ctccatagtt
     6421 caatctgttc agcaccttga taaagcgaca tgcccaagcc attaattatc ttacagccaa
     6481 cttgttcagc ttctgtaagg aatctggtct ttgacggatt ataggttgca tcaaaacaca
     6541 actgcacagg actgagatac tctttgggaa tcggagtttt gtccaggctt gcaggcatgc
     6601 ccaatccggt attgttcatg ataacatttg atgctgctat acttttcttc agcaactctg
     6661 tgtcctgcat atccacctgt tccgccacag gtgcaaactt ctcattgatg tcttcaacca
     6721 gttccttgac tcgtgataag gttctgcttg caatgtatat cttctttgct ccgttatatg
     6781 caagtacact gcatatagct ctggcggcac ctcctgctcc gaggcagaag aatacagatt
     6841 cagagatgtc tatttcaggt gcttcttcct tcagagatct taagaaaccg ataccgtcag
     6901 tgttatatgc cttaaggcgt ccgtttttaa ttactacagt attgcttgca ttcatttttt
     6961 cacatagagg atccacttca tcaacatatt tcatgacttc cactttgttt ggcttggtaa
     7021 ccgcaaatcc ggcaaaattc atgtgtctga ttccattgat tacatcgggt aaatgatcat
     7081 tttcaacttc tgtgtaaaaa tacagcatgt cgattccggc tgccctatac gccgcattct
     7141 gcatcctggc tgcataggac tgagatagag gttttcccag cagggttatc atcttggtat
     7201 taatattaac cggcattttt ctcccccctt tttatattat ataatatcaa agaccactgt
     7261 acctttatct attctatgcc attttcggca aaatactgct ttgcctttat caggcattgg
     7321 gcagcatgcc caaatgctcc tctcttttcc atctccttgg aatttggcaa ttcaattgat
     7381 attggatttg ccggcattgc aagaaacata ctctttaaat cgatttctcc atcccccgga
     7441 taaagccttc cctctcttgc aactcctatc atgcccggat catccaatgc aggtatggga
     7501 cccgggccgt cacaaaggtg aataaatcca aattttgaag gttccacctt agcaatatct
     7561 tctgccgata cccttgacct gtgagcatga agcgtatcca ccatgaggta tgcattggga
     7621 cgttctactg tattgagtac atccgaggct tcaaccaggg atgtaacccc tgcgaaggtc
     7681 acgaactcca gattcacacg aagagagtac tttgcagcaa gatcgcatat ttttgcaaat
     7741 tccctataat aataagcctt gtccttagtc catatgcttg aaagcacatc tgtccctcca
     7801 agctctgccg cggcaaaaaa tgcagattca taatcctcaa ccttcaagtc ctctcttact
     7861 ctggccagtt cgatgtccaa cagcctgata ttattttccg aaagcgcatg cttaatctca
     7921 ttgaacagct tcttatcctg atcaaaccgg aaaagaggtt cgttagggag atgcatcggt
     7981 atcgggcgga ggctgacgaa atcatatccc gcctctgcag ctatgcgaat ctgatccacc
     8041 ggaccagtcc cgggtattgt aagataagca agggaaaact ttcttggcat aattgctctc
     8101 ctttcaaata cactgtttat ttgtgtgaac ctgaatggtt cggagcttca tattcacctt
     8161 tctcattctt tataattttg ccgtttacaa tatttgcttt aggtgtatat atgtcattga
     8221 tattccagca gccgggtgta ggtaccccaa tattctccat aggcacatcc aacaggaaag
     8281 gcctattgct ggccaatgct tgctccaaag ccggcttgaa ggcttcagcc gaatcaatac
     8341 gaattgcatc caccccgtat gcctttgcaa cctctgccca gtttggagag tatggtttcc
     8401 catccggtgt ggtaaatacc gttccaaatt tggtcttata gttggaatac tccaaaccgg
     8461 caattgtacc gaatgcggag ttgttcataa caatccatat tacggcaatg ccctgttcta
     8521 ctgctgtggc aagtgagctt ggattgattc cgaatccccc gtctcccaca agtgtgataa
     8581 caaccttgtc aggtgccgca agcttgccgc ccagcagcgc cgacgccccg aatcccatgg
     8641 ttgcaagccc cgagctgtgg tgaatggttc ccggaagagt aatgtcaaac tgctgagcca
     8701 cgccattctt attccagcct acatcggtaa atatgattgt attctccggt atcgtttcct
     8761 ttacgtcctt cagtatacgc tgcggtgtca ttgggaagcg cccgtcttca gatattgcaa
     8821 tatttgactc tctgaataat gtacgattct ttttgatcat ttccctgagt tccggccttt
     8881 ttatgccttc cgggcacagc ttttcagctt cttccagaat ctgtgcaagt gcttctttta
     8941 aatctgctgc agcaccaatg ctaaccggat aattgcgtcc gatttctgtt gggtctatgt
     9001 caatttgaag aaattctgtc ttcctcatgt caaaggttac gcccgggtac caactggagc
     9061 tgtcagcctc cgcaaagcgg gttccaagcc caagcagaac atctgcattg gctgtaaact
     9121 tatgtgtaaa ttcaaggccc cagaatccgg tctgtccaag catcaatgga tgagtatcgg
     9181 acatacagcc ctgccccatc agtgtccggg ataccgggat atccagaaac tccaccaaat
     9241 cctgaagctc ttttgacgct cctgccagaa gtataccgcc gcctgcgtga ataaccggat
     9301 tcttggcttc caccagcttt tgtgctatag cttttgcagc tttaggtgcc aatgcaggtt
     9361 tgatagtctg gtgtctatca aggtaagtac gtgcgaaaag atcctcttca acctcttcgg
     9421 agaaaacatc cattggcaca gatatgagaa ccggcccggg ccttccgctt tcagccatgc
     9481 gaaaagcttt atccaatatt tcaggcatag cctcaggatc atcaactctc caggctctct
     9541 ttactatggg tttcaggatt tcatattgag cagcgtcagc atgcagatta acttcctgat
     9601 gaggatgctt tccgaaataa tatcttggaa catctcctgc tataacaacc atgggtatgg
     9661 aatcaaatga tgcattggcc acccctgtta cggtatttgc aataccgggg cctaagtggc
     9721 acattaccac tccggccttt ttcttgattc gggcataccc gtctgctgcc atagctgcaa
     9781 gctgttcatt tctaaaggaa acatatttca cactgctgct cctggaaagt gcatccagga
     9841 aggcaattac cgtgtgcccg caaagtccga atacatactc gaccccgcgg cgctgcaggt
     9901 aattcaccat ttgttcggaa atcaattttt tcaaggtaat acccccttct atacccttcc
     9961 ttctctctca ggctcgtaga attcaccgaa taattcttca tcactgggct tatcttcagg
    10021 aatagctttt gatagccatg taacattcat atagtggcgt aaatttacgt tttcggatgt
    10081 tgcattccct ccccaggttc cgcatccaat gcttgaggtc ataggcattc cgttggtaaa
    10141 ggagcctgca ttggattttg attgcggctg tctcaccatt atacggccta cacgggcttc
    10201 aagagccagc ctgtgtatat gctcttcgtc atatgagtat atacctacac tgtggccttt
    10261 tccgcctacc tcatggatag ccattattat gtctattgca ttctgaaact cgccttcata
    10321 tttgtgtaca gccagcaaag ttgtcagctt ttcgcggcac catacatcct catagcttgt
    10381 tgtgtatgat cctgtgccta tggctatgag gaatttacga tcctccggta ttgtaaaccc
    10441 ggccagctca gccattcttt gaggcgattg ggctactgaa tcaggaagac gatgacattc
    10501 ctcatcccac ataatcttct tgacagccag ctgctgttcc tcgctcagca ggtagcctcc
    10561 cacattgacc agttccttta ttgcatcatc atagatgctc tcatgtataa ctatatttcc
    10621 gtcagctgag catccggatc cgaaatctga tgtctttgaa agcattgtat tgtacgctgc
    10681 ctggtgaagc tcatccttag ctgtctcatc ccaaatcatg gtcgcattgc cggcccctac
    10741 accaaaagcc ggagtgcccg aatgatatgc tcttcttact acaccctgtc ctcctgtggc
    10801 aattaccaaa tcgcatttcg tattaagcgc ctcagacatg gctttgttta ttttagcttt
    10861 tccaactcca aggatctgaa tcagatcttc cggtgcgcct acctgcctca aaccttcacg
    10921 cataagctca attgttcggc ctccggtttt tactgctctt ggatgtggtg agaaaattat
    10981 ggcgttccgg gctttaaggg catatatggc attgcctgcc ggtgtcaagt ccgggttggt
    11041 agccggaaca acacatgcaa tgactcctac cggcttgcca tacttgacta tccctttttc
    11101 cggtatttcc tcaattatac ctacgctctt ctgacgcaac gcatctctca gcacacctct
    11161 gattttaaat ttcttattct cgcggcttac ggggtctccc atcccgctct cctctatgct
    11221 catcttgacc agttctttaa aggtctgagt atttgcaact ttccatgcta tagctcttat
    11281 agtgcggtca acttcttcct ggctccattt ttcaaatatc ttctgcgccg catctgctct
    11341 tgcaaacttt tcctcgattt catctatctg gtcctggtta agctttgaat atacttcttc
    11401 cattgaactc atcctgctca taatcattcc tcctaaacat tagtnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnccgggg acaaaaggac aacctcccag ccctgcgaag
    11581 gaggagtcga attgtgttat tccggcctgc atcgcagcca gtatatttgc catggccatt
    11641 cctcttgtat tatggaaatg aagccaccat ttcacctggg gatagcattc ctgcatattg
    11701 atgcacattg aatatacttg attgggcaca gccataccgg aagcgtccga tagggatatt
    11761 tcgtctattc cgacattcag gtaggcttca actatactct tgacagattc cagcgatatg
    11821 tttccctccc agggagagcc aaatggcatg gaaattgatc cggatacctt aattccatgc
    11881 tgctcggcaa gggcaacact ttcttcaaag ccggcaacgc tttcctcagg tgtcctgttc
    11941 agatttgcca gattatgccc gaggcttgcc gacacattaa gcttgacctt gcggcacccg
    12001 caattaatgg cgcgttctac acctttttta ttggcaatta gtgcacgcag ctctactcca
    12061 tcaatctgat tcaaagttga gaaaagctca tcggtatctg ccatttgagg tactgccttc
    12121 ggattcatga aagaacctaa ctcaattacc ttgaagccgg cagctataag accattggcc
    12181 aattccagct tttcctgagt ctgcaaaatt tttttttcat tctgcaagcc atcccggagg
    12241 ccaacttcac aaatggttat ttttgacgga agattcatac ttcttccctc cttataagtt
    12301 ataagaatgt tttatatgtt aagaaaagtc tatattaatt tctctcaaca gtccaatact
    12361 cattttgtat aatataataa aatttctcta tgacttgttt gcgtttgaaa tttaatctct
    12421 cttcactcac ctgcaaattg accggttttc tagggtttgc tttttaaatg atttttttat
    12481 attgtgaaaa ataacttctt cgatatgctc aatgaccttt ttgtacattt tcaattttat
    12541 ccttttttct ctaatatttg caattttttc atatgatttt ctgaatattc attatattat
    12601 tataatttta tagcattttc ccttgactgt ccaataccca ttttgtataa tttaatagaa
    12661 gttctctatg acttgtttat atctccacaa tgagtatttg acttttgcag caatgaaaat
    12721 ttaaacttct aaggaggcga taccatgacc ttggaacaat taagatactt tgttgaaaca
    12781 gctcataccc tgcactatac caaggcagcc gaacaactga atatatctca gcccagcctc
    12841 agttatgcca tatcccagtt atgcaagaga ttgggtgtac cgctgtttaa aaaggaaggt
    12901 ataaaagttt cattgacaga atacggagaa gcctttctcc cctatgctga gagtgccctc
    12961 aacatactta tccagggaga agctcagctt aacagaatga atagccctta cggggggaac
    13021 attaatctgg gatacattta cagtgtcagc tttgatgaaa ttccgcattt aatagatgat
    13081 ttctacgcct gtcagggcaa ccgcaatata agtttcagct ttcaggttaa catgacaaat
    13141 ttactgatca agaagctgat agaaggcacc cttgatgttg tcatggctcc atctactgaa
    13201 attgtgaatg agtgcatcga attcatccct ctatttgaac aggagctatt tctggttgta
    13261 tataaagatc atcctttggc aagtcatacc tcagtcacaa tagatgattt caaaaatgaa
    13321 aaatttatta tgataaaaaa agaaaccgat ctccattttc agaccgattc cttatttaag
    13381 aaattcaata tcgtgccgaa tatctttttt gaggtcgacg agtgcaactc aatggcctct
    13441 tttgtaggag cacagctggg aatagcgatt atgcccaaaa ttccttctct cgatggctat
    13501 aaggtaactg ccataccctt tgaggggaga acagtgagaa ggactatcag tcttctgtgg
    13561 aataatagaa taaagcatac tcctgctctc aaaagcttcc tggactattg catctcttca
    13621 aggtcaaccg gcataaagaa tacagaaaag taatggcgcc aattaacgcc tgatattttg
    13681 attcagttaa tttattcgta ataaaattca atttcaatgt cttttccgtc gaaataaaca
    13741 aggtcgcaat catcgtaatg cgaaatgtgc caatatggcc gcaatatctg aaatatttct
    13801 gaacaggcag cggcagatca gcaatatctt cttttgtaaa tataccgtct tcaccaggca
    13861 gtccggttaa ataacctctt tttcatatac aatcctccac ctcagaacca ttctctaaca
    13921 taacaacagt attgtaaaat cacacacggg ctattttggg cacaacctcg tttgcaaagc
    13981 attcaaccaa tctgttcctt tgctcctctc ccgtctcttt ctcagcaata agctttataa
    14041 caaggcccat ggcagatgca ataattaact gcgaaataag ttcgcatttg ctgtcctcca
    14101 aatcaatatc cttgttaatt tcttttatgc atttgcataa tgcacctatg acggactttt
    14161 catcagaagc cccctcatat agaaatgaag tctgctttat agtttttgga gatttgctta
    14221 attgcgcagc catatactct tcaggcattt tcagtgcggc gtctatataa tacctagcca
    14281 ttttttttaa ttgctcttcc ggagaatcac ttatcatatt tgcagaggct attgccgata
    14341 ttattttttt ataacctctt tgcatcaaat ggcttatgat ttcatcctta tccgcaaagt
    14401 agtggtaaat aatagatggc gagtattcaa ttttgttagc tatttttctg atagacagct
    14461 tatcatatcc ttctgaattt gctatcatag ctgccgtaga cagtatgagt tccttcattt
    14521 cttctatttc acgttctctt ctttcactga tagccataag ttcatcctcc gtttagcttc
    14581 gttcaattat ttacctttgt tcttttttag aacactgttc tatttcataa tatctctatc
    14641 taatcctgtt gtcaatacaa aaatcgctgc aaaaaaaata agtgcccggc aaaatgaatt
    14701 gccaggcacc agacatatat acttatcttt tccaattgct gataaaatcc ttaaatttct
    14761 cctcaatagg tgaaaggcat ctgttattgc aataaaccag gctaaagctt ctcttaagct
    14821 ccaggtcgga tatttcgtat tgctttagtt gaccggactc aacttcacgc tttactgcaa
    14881 tatcggatac aagactgata ccgacatcca attccaccat ttttttgatt gcttccaggc
    14941 tgtcattaat aatctgagat ttaaacatgg acatatccag ttccttttct ttcaaggcat
    15001 tttcaagaag aagccttgtg ccggaacctt cttccctgag cagtatgttt tctccgatta
    15061 aactcctgat actgacagaa tccgaatcaa attttttttg aggcgaagca ataagtacca
    15121 ttctgtcatc ataaaaatca atatactcaa gggcatctga gctatacttt gctccgacaa
    15181 gccccaggtt aattctgccg gaaaggattt cttccattac ttcgcttgaa tctttatggc
    15241 agatagaaaa aaggaccaaa ggataagctt ttttgaattc tttaacgata tacggaagaa
    15301 gatattgtcc cggtattgtg ctggcatata actccagggt cccttcaata ttctttttat
    15361 atccgctgag ggcgtattcg gctttttcat aaatattaat taattccacc gcataacggt
    15421 aaaaaagttt tcctccttca gtcaatgaga tattcttggt ttttcgattg attaatgtaa
    15481 ccccaagttc gctctccagt ttctgcacat tactggtgac agccggctgc gatatgaaca
    15541 acatgtccgc cgcttttgaa aaactctttt ttcgggcgac cgcaacaaat atctttatct
    15601 gattaaattc cattttatcc ccctccggtt acttcagttt atatatttgg tccgccgcat
    15661 ataatatatc ggcttcctcc atgaaatggc tcaatccgaa acgtactgta ccccttggga
    15721 aagtccccaa tgtcttatgg gcggaaggcg cacagtgcag cccgcttctg gtcatgatac
    15781 cggactctga agagaggcgg tgtgccatct ctgcattatc ctgtgtctta aaatcgacag
    15841 atacgattcc tgttctccct ttagtatcct tcttcccaat tatatcaata tgaggcatat
    15901 tcatcagcct ttccaagaaa actcccataa gccgcatttc tttttcatat atatcttgaa
    15961 tacctgtctc ctgtatatat ttcagtgaag cgttaagtcc gtaaataccg ggcacattcg
    16021 gagttcccgc ttcaaattta tccggcatgt aggaaggctg tatttccata tcagacaagc
    16081 ttccggtgcc tccttcaata aatgcggaaa gttcatcagc caagccttcc cgcaccagaa
    16141 acccgccaat cccctgaggc cccagcaagc ctttgtgtcc ggtgaaagca acggcatcag
    16201 cgcacatatc atcaaagtca acctttatag ctcccgctgt ctgtgccgta tcaacaataa
    16261 accgcagacc tttttctttg cagatacttc cgacctcttc caaattcaaa attgtaccgc
    16321 aaacattgga agcatgagtc ataactactc ccttcgtatt gggaaggatt agaggcaata
    16381 aatcctttcc gtcgatgctg ccgtcatttt tgcataaaac tcttgaaaat gtaacccctt
    16441 ttccttcaag agccctgagg ggcctcatca cagcgttatg ttccatggag gacactatca
    16501 catggtcgcc gcttttaaaa agccccttaa ccagcacatt aaggctttct gttatattct
    16561 ttgtaaatac aacgttttcc ggcttattga aaccaaaaag cgaacaaagt aactccctcg
    16621 tctcaaaaac tacattttca gcctgaaaag aatttgaata agagcctcta ttgatatttg
    16681 cacctacatg aataagatat ttgtatacac tttcagctac acccggggcc tttggaaagg
    16741 atgtagcccc attgtccata taaatttctt tcatagtgaa cctcctcgca tagtcatcca
    16801 tattttagat tatacattgg aaaaaacata aatacaattt gtattcgttt tcataattct
    16861 acagcacgtc attatttgtt tttgttattt tactattgtt gtttgcattg ttaaactaat
    16921 aacagaatta tctataatta taccattaag ggagatattg aagtatgaaa gaaaaaacag
    16981 gaattattat caccggtggc gtagttggct tatttgcagt tctactagtt cgtttcggca
    17041 atccggcaaa catgggtttt tgtattgcat gcttccttcg cgacatagcg ggagcacttg
    17101 gtcttcaccg tgcagaagtt gtgcaataca ttcgtcctga gattattggt cttgtattag
    17161 gttcattcat tctagcctcc atgaataagg agtttaattc tcaaggaggc tcatcacctg
    17221 ttaccagatt tgtactttca atcatcgtaa tgatcggggc acttattttt cttggctgtc
    17281 cccttcgaat ggttttgaga attgccgggg gcgatttcaa tgcaattatt ggattgatcg
    17341 gatttgtttc tgggatttac gttggtattt tctttctcaa caaggggttc aatcttaaga
    17401 ggagttataa gcttacaaat ttggaaggat atctgttccc ggctattaat gtcggctttc
    17461 tggccttgct tattgtagca cctgcttttg tgtttttcag taaatccggt ccgggagcag
    17521 ctacagcacc gatatggatt tcacttgcgg caggattaat tgtaggtgca cttgcgcaaa
    17581 agtataggtt gtgcactgtc ggtggtacca gagacttgat tcttttccgt gacaactatc
    17641 tgatgcttgg cttcttgtca attcttgcag tagctttagt tggaaactta attttgggta
    17701 aatttaatcc cggttttgcg gagcaaccta ttgcccatac ggacggattg tggaatttcc
    17761 tgggcatggc attggtagga tggggatctg tacttcttgg tggttgtcct ttgcgtcagt
    17821 tgatacttgc cggcgaaggc aacactgatt cagctatcag cgcattagga ttgattgtag
    17881 gagcggctat ttgccacaac tttggactgg catccagtgc aaaaggagtc actctaaacg
    17941 gacagatatc tgtcggaatc tgctttttaa tcctattggc aatctcttac gccaattcag
    18001 agtttctatc aaaaaacgca cagtcttaat aggaggtaaa tatgaaaaca atagatacct
    18061 gcggcatgag ctgcccgcaa ccggtactta tgactaaaaa agctcttgat gaggaaaaag
    18121 acggtataga tgttatagta gataataata cagcaaaagg gaatgttcag cgttatttga
    18181 aaagtgtcgg gtattcagtt gaaatcgaag aaaacgacgg cacatttgtg ctaaaagcca
    18241 gaaaatgaac aaagaatatc aagttctttt ttacacccat accggggcga taaaattcga
    18301 gcgggctatg aaaaaagaaa atatctcttg tgagctgatg cccgtaccaa gatcactaag
    18361 ctcaaactgt ggtgtaagcg cccgtattta ctataatggg caagttgacc aactgattaa
    18421 tgatcaggta gagaaaatct atcaaaaata tgacgggaat tatatactta catatgaagc
    18481 agaaaaatga agtaattgcc aggcacgcct aatattttgt ccggtgcctg gcatctgttt
    18541 atcttcgggt gaaattccgt tagtatgaaa tctttttctg caaaaaagta ccatttttca
    18601 aatcatcgaa agctttattc aattcttcct ttgtgttcat aactataggt cccccccaag
    18661 caatcggttc agccaggatt gtcgaactta caaacaacaa ttgcgcattt ttgtttgttg
    18721 ctattatctc cacatgttca ccggaggtaa gctttaccgc cgtcttttct tttactgatt
    18781 cgcccccgat atatgcatcc cccaagagtg taaaaatcat taccgagcgg cttctttccg
    18841 tttttacgga aacagaggta tcggcattta gatgaaggtc ataataatcc agcggaagat
    18901 atttgctcat ataccctttt cttccctcat attcgcctgc gagcagtctt agctttccgt
    18961 tctccaactt aacttcttca atttcagcat tctttatgct gcggtaagcc ggcggaacca
    19021 tcttatcctt cgcaggaaga ttaagccaaa gctgtacccc aagcaatctt tcggatgccg
    19081 gcagcttttc ttcatgcaga atacctgatc ccgctgtcat ccattgtact tctccatcgc
    19141 cgatggtatc ctcattgcct aaactatcct tatgggtcat atacccacga tacacatagc
    19201 tgattgtttc gatgcctctg tggggatgca tgggaaatcc cgctgtgtaa tcatccggat
    19261 ttgtactgtc aaaggaatcg agcattaaaa tcggatcata ttcactagca gtcccctttc
    19321 ccaatactct aaccaaattt actcctgcgc cgtcttgtgt cctaaaccct ctaacctgtt
    19381 gttcaacctt tctctccatg tgtttacctc cataactcat cacatatgtt ctgcaaaact
    19441 aatacttcta ccgtgggtat tggccggata atagtgccgg gcactaagat ccaatgatac
    19501 gaaacccttc cggcagcgga tcatcaggat caagcacaat attgttgaag ccggtgatat
    19561 gtgcggtacc tgacacttcg gtaatcacgg cgtcatattg gcccactttg cagcggctta
    19621 tcacttttga ctccatagga gtgtctatta cacttttgtt tacaagtgtg tcatcctcct
    19681 gcatgatatt cttgtgcacc aggagtgccg tacgtcctcc ggtgcctgtt cccgtagggc
    19741 tccggtctat ctgtccctcg gcaaaaatac agatgttgtt cgatatcagc cgattcccga
    19801 tacgttcgac cggcgacatc agaatagtgc catacagcca gtcaataccg ggttctgtag
    19861 ggtgtttgaa tgtatattct tccatcactt tgtgttttat ctccatacca atcttcacca
    19921 gctgctcggc gtattggggc tctacttcca aatcaaccct gtcggcgtca atgtacacat
    19981 agaacgcacc gcaataggca atgtcgcaga ctacatccct gttcaggctg tcaacatgta
    20041 cagtgatatt ctccttatat acaaaacagg gcacattatg aaagcgcacc cgcagcacct
    20101 tccccttctc aacatctgcg tacgctgtaa cgcggccggc aggagagtca attcttatca
    20161 cattctcacc ttctgtgcag cctatcatcc cggtttcaat tactaccttc gtcacgccga
    20221 taatgccgtg cccgcacatg gaactgagcc cctcattgtg ggtaaacagc actccgaaat
    20281 cgccatccgg agtaacagca ggtacaagaa tgcatccata cattcccgag tgtccgcggg
    20341 gctccagcat cagcatacgg cgcagtccgt catagttctt atttacatag tcgcgttttt
    20401 caaggattgt attgcccggt atgggcggaa aacctgctgt aataatgcgc agcggctctc
    20461 ccgccgtatg tgcgtcaata catgtaatat agtgggaaaa attcatcata aaaaaccctc
    20521 cgttatttat tggaattgct ttgcggttac ggatttaatt tttggccgat accctgctct
    20581 atagccttat tatatatatt tccggcaaca aaaatatcaa acagcgccat tccgacagat
    20641 ttgcagaacg tagtcctgct gcggtcaata ctgtcagctt tgacaactga atagagtgtc
    20701 cggatatttt cttccttgaa ccagcctttt tcacaaggcg tcgccaaatc tcccgattcc
    20761 tccctggcaa agtcaacatc tatgtaaatc ttttcaagca atttgtaaat gctctccgag
    20821 tactcccgca tattgggttt gtaagatcca atgcctataa aatgcttccc ttccaaaagt
    20881 tcagcgtcat caggcagcac aggagtaaaa gaggtcgtat acaatctatt gtgccggttt
    20941 ttggattgtc gagcagcatg accccctgta cgtcggcaga ttgtatttgg aattctcctg
    21001 ggacagggta aggatcctgg ttccttttat ctattcggtg aagcagggca tatagagata
    21061 tgtctccgta tcggtcacat tgacttttat gcgatccggc tgcataaatt gcttcttgtc
    21121 atagatttcc acagagtgtt ctaaggcctc aatcattcat taagatcaat gagtgaagaa
    21181 atatcttgtc tatttaggta taacatgctt agcctcctct tattccaaag gctgttcctt
    21241 tttgtcttcg gacactatct tcaaaggtat aattgtaaga acacatttta aggttatcga
    21301 aaccaaaaca atagcacccc ctaacaatgc ataaataccg ggagcctccc ccagcaccag
    21361 aaacaccaat acaggattca gtatgggttc aactatggaa atcagcaccg cttccagggc
    21421 tgtcgctttt tttatagctg aagaataaag aatatggggt atgcccagtt gaaatactcc
    21481 aagtatcagc aggaaaagcc aacctgaagt gcttggtaca gaccggcaga gaaaaggaag
    21541 cccaataaga gcggtaataa tatttgccag cagagccgat tccaagggag aaccgtcttt
    21601 ttgcatcctc ataaaaatca caaaaaatgc ataactaata ccactcagca atgcgcaaaa
    21661 attccccaac agtccttcgg tactcaggtt ttcaacaaag aataatccca tcccccccat
    21721 taccacggca actgtcagcc aatcgtattt tgtggtcttt tccttaagca gccaaaaccc
    21781 aaaaatggca acgtatattg gggcagtgga ttgcagcagt attgcgttgg ctgcagtcgt
    21841 cgtctttgtt gcagtaataa agagtattgc tgtagctgaa ctggaaatcg ccgctcctac
    21901 ctgtggaaga gaccagttaa atttcggtct tctgaggtat ataagcagaa ctattgatgt
    21961 gatggcagac cttgctcctg caatagacag agggctggca tccacaattt tgataagcag
    22021 tcctccgata ctccacatcg ctgcagtggc agcaagcagc agcattgctc tggacctttc
    22081 ctttgataac ggagcagtac tcaatttttt tcctcctatc ccctaagtca atttccctta
    22141 taactattat atcatttaat catctgagac aacatggcgt ctatagtcac atctttttta
    22201 agtatgcctt taatcatttc aggccttatc tcccacatgt ttgcctgtat cctgaaagga
    22261 tcccattgat ctgtctcaaa tactcttatc caactgtcca ttaccctttt tctttcctcc
    22321 ggataaaaat gtgaagtatt ctcatcaagg aaagaatatg aatccctgaa gcctttatcg
    22381 gatatatccc tttcatactc ttccaaatcc ttatcatcct tcggcacata atgatggttc
    22441 agcacataat cccatttaca gccgtcaaaa tatattattt ccgattcatc cacctccagg
    22501 acatacacta tctcagtgtc cgtgggcctc agcatatatt cctcactaat ggaacaccat
    22561 atcggaaact ccactccctc cggcttggga accatcctat ctgcagcatt aacaaaccaa
    22621 ctgtataaat tgatgatata gtctgcaatc agatcaaact tttctaccag atgctttctt
    22681 ttgatacgaa ggacaccctc ttttttcaaa tcctccaggc tccttatatc ctgcctcgtc
    22741 cagagggtca ccttttttga tgaagctttt tgttctgcca atcccattac cccctaactt
    22801 tgtgttaggc acaaaatcta agaacttatc taaattgata catcgcttaa gttctattat
    22861 tctgtatccg gcacctaagc aaattataca atcttttcac cgttgtaccg ctctccccct
    22921 gcattgggca aaagtccata acgctcggtc cacaaggttt tgtattttat agcctgagca
    22981 tgaatctgct cccacgatcc gggtatttgt ttaataacct tgccaggtac ccccaccaca
    23041 agcgaatgcg gcggtataat ctcgttttcc agaataacgc ttccggctgc aactatactt
    23101 cccttgccaa tgacgcttcc tgcaagaata actgaattca ttcccaaaag gcaatgctcc
    23161 tctatggttg ctccatgaat aatacaacca tgtccgacgg taacataatc gcctataatc
    23221 gaagcacaat catctgtgcc atgaataacg ctattatcct ggatgttgct atagcagcca
    23281 atctcgatag cgttgacatc gcctcttgct accgaattaa accaaatgct gctatactcc
    23341 ttcatcacta ctctgccgat aagctgaaca ccttccgcca caaaagattt agtatccaga
    23401 ctgggagtgt gggattcaaa tctatgaacc atgctatgca cctgctttca atattttttc
    23461 gtttatgagc gccggccgcc aaaattatcc gcttgccttt ttgtgtccgg caccgacata
    23521 atctattcct gctgcctttg cctgttttcc aagttgattg tacccaaggg catactcttt
    23581 gccgtccttc ataaaaaagt cctcctggta acatattatc ttagtattat caaaaggaca
    23641 acccattgcc ggcattccaa attatattga agaatattgt aattcggcta taaagagtac
    23701 tataataaaa ttggaacaat acaaataaat tttaaggggt tgaaatcatg caattagaga
    23761 aacagttgga aaaaattgta ggacttatgt cagatattaa tgcggatatt catatgattg
    23821 gcccttcctc cggccttgaa tatctgacgg gaatcaatcc tcatccggat gaaaggttca
    23881 aagcgctgtt catcctaaaa gataagaggc atttctacat atctccggaa atatactatg
    23941 aagaaacccg ggaactcttg ggagaaaagg aagacatatt tgtatggagc gatacagacg
    24001 gcttcctgtc agcaatcgag aaagccgata aaaaatatgg ccttgcagga aaaaccattg
    24061 cggtaaatga cggcatcgta gctataaata tgctggatat aggaaacttc ttagacgtaa
    24121 aattcataaa cggaaatagt atactggaag atgtcagaat ccgaaaaacc caagaagaaa
    24181 aggactgcat gagaaaagcg gcaaaaattg ccgatgaagt ggttgaagaa acaataaagt
    24241 ttataaaacc gggtttaaca gaaagagata ttgcaaaaaa gctggaagaa ctctatacgg
    24301 aaaaaggtgc aagcatgtcc ttccacccta tcgtggcatc agggcctaat acttcgaagc
    24361 ctcattacaa tggggacagc agggtcatac aggaacagga tataataata atcgataccg
    24421 gctgcaggta taaaggctac tgctcagata tttcaagaac gattttcgta ggtgaaccca
    24481 cggaagaaca aaggaagata ttcgatatct gtgtaaaagc gacaactgca ggtgaaatgg
    24541 ccgtcagaga aggcatcact gcagaagaag tggatatggc tgcccggaat attataaaag
    24601 cggcaggcta cggggaatac ttcctcaaca ggaccggcca cggtataggt attgctgtcc
    24661 atgaaggccc gtatataaga accggcaaca agcagatcct cgaaaacgga atgtgcttca
    24721 gcgtagagcc cggaatatat atttccggaa atttcggcat gagagttgaa aacatagttc
    24781 tggtggaaaa cggtaaggca gaaatactca acaaagtcac caaggacatc attattgttt
    24841 aattttaagt gcaaatcaaa taaaaaaccc tatattagat ttatgcgaac atattcaaaa
    24901 gcactgcata tcaaaatgca gtgctttata tgtaatttca aatattttgt atttagagta
    24961 cgagattgat acaattcaat tttgtctttt caggggggtg caaaatagcc gaggggggtg
    25021 caacgctata cggctactac tattaacaag tagtttcaca tttagtaata tattctggtg
    25081 tcaactcaat gcgagtgccg tcacctttgg cgaggtgctt aaatcctgcc tgccagagca
    25141 ttttacggca agctgtgaca tccaagccac gtcctttcga ggtgtccgct tctgtatcac
    25201 gaggattgac tcctgtttca gcgcagatgc tgttggcacc gctgacaaga ctgatataat
    25261 ccgcttcatg cacgggaatc caaggatacg gagcgatggc cgtcatcgca agggcattta
    25321 cggaagcgat ctgggccaaa cgcgcatttg taatctcgtc tgtgaatgaa gtttcaggta
    25381 ccggggtgcg cttcataaca cctgcagtat cgacgccttt ttcaatggtt ttgaaaatgt
    25441 gatccaccag ttcctccggt gtatgctctg tgccgatggg ttccagacag tcctgcagca
    25501 tcagcccggc acggcgcgca ttgtcgatgg tctcctgacg gcgctgagga ctgatggtcg
    25561 tataagtgcc ttcaccgagg cggatgacat ggtatgctcc gtccgctccg gctttcttca
    25621 gtttgacaaa cgtctcgtaa tcggtatcac cgatattcgt atacagcaga gtctgttttg
    25681 gcgcagcagg tacactgacc ggctttgctg actgtaattc ttgatttctt tagagaactt
    25741 actatcatca cgttttgata tatatacctg gtaagcatcg atactcgaag tatcatcgca
    25801 ctcaaaatca ataaatgcgt catctttatt aaacgatagt ttttttaaca actcaaaatc
    25861 gtagttcatt gaaaatatag gcgtatattt atcatcattc aactcataaa aagacccctt
    25921 ttctgcctcc ggtataagct tgaaagtcgc ttcgaatacc ctctgtaaaa aactcctctg
    25981 acttaattta tgtgcatcat ccagtgagtc aataatataa gaaacctttt catcaaaaaa
    26041 actattgttc tccgatctat actttattcg tcttatatta ttagtatctt tcataaacct
    26101 ataagtcttt tgcacaagaa tccctccatg aagcttataa aataataata tcatatttta
    26161 ccagtttttg tactattttg acgtttttaa taccattttt cctatagcaa gaacaaccag
    26221 gctttcatat tctcaaaaac tctgcggagc gtttattgaa tagttaaatt ctgaaaatta
    26281 tattagatat atagaggggg aattaaatgg attgtagcaa agttggcgta ttgatactga
    26341 gcttgcataa agaaaaaaac atgacccaaa aagatgttga cgacagaatg aatataagcg
    26401 ataaaaccat ctcaaaatgg gagcgtggca tgggctgtcc cgatgtgact ctgctcggcg
    26461 acctttcagg catatttggt gtcaatattg aaagaatctt aatgggtgac ttggaaggac
    26521 ttcagtatga aatggatagc ccggaggttt gggcatttga ataataagca ctgtattcgt
    26581 ttccagaata cagtgctatt ttatataaaa tcaattattt ttggcggaga gagagggatt
    26641 tgaacccctt tcccttctgc atgcctatac aacaagggaa cgggtatgct cgaaatcttc
    26701 atattcttta cttatggtga gttcttcaag tatatctaca agcctgtgga catcttcata
    26761 tgaaacatat aaagctacag gtgccaatct aataacatca ggctcgcgaa aatcaggtat
    26821 tactttatgc ctctttaaag cctggcagat ccggtaagcc tcctgatgct ccaaagccac
    26881 atgtcctccg cgtttatcat cttcccgcgg attgccaatg ctgtaaccat attttgtgag
    26941 gcgttcatct ataagataca ttagatagtc agtgattttt aatgattttt cacgaatcct
    27001 gacaattcca atttcatcat aaatatccag tactccttca attgctgcca tggaaagaat
    27061 cggaggtgtc cccgtaagcc aaccgtcagc atcatttgaa tgctcatatt ggtgcttcag
    27121 ctggaactgg gtggacttgg tgtttccgaa ccagcctgtt aatcccgggt ccatcgcaaa
    27181 gtgcttctta ttaatgtata atccggcgga agttcccggt cctccggaca tatacttgta
    27241 attacaccat actgcaaaat ccggattgat ttttttaaaa tcatgaggaa taacgccaat
    27301 ggaatggcaa agatcccagc ctatgtaaat acctcgcttg tgggcttcct ttgttatcct
    27361 ctccatatca agcaattgtg cactccgata aagaagagac ggtagtagaa tcaatgccac
    27421 atcatcagtc atagcttcaa taaccgcatc ctctgaaatt attctgccat ctgggctttg
    27481 aactactttc accacatcag cgggatcgta cccatgcagc ttaacctggc tgtctactgc
    27541 ataacggtct gttggaaaat tcaaatcatc cactaatatt ttgtatttat ccttagtcgg
    27601 tttatagaag gtagcaatcc cttgatgaat attgattgtg acgctgtttg ttactgttac
    27661 ctcattggct tcagcattta tcaaggaggc catacgccct cctaaatagg aaggataaag
    27721 aaagtatttg ctgtcttcaa cattccaaag tccaatgcca tgttccttcc atacatccat
    27781 aaccttcaac acagcttttt ccgcatcctt ggagcaaagt ccaagagaat tgccgtccat
    27841 gtaaatttca tcctttctta catagaagcg gttgcggacc tcctttaacg ggtcctgcat
    27901 atccaattct tttgcacaat ccaaaccaag ccggaattca tgtttcattt ttatccacct
    27961 ctcttaatag tatcagcaag aaaaatttat ttttccgcaa agtcttatgc aacctctgac
    28021 ataactttac cggaatcttt caaaaaataa ttaataaact aaaaacagca ttaagcatat
    28081 ttatccagct tcaattttaa taatcgtata gtatgataca ttccgacaat ttcattttat
    28141 tcttttaaac aacattattc aagcaaataa taagtacttg gcagccctgc actaatctgc
    28201 tgcgcagaat tcccagttta catcacctgc ggcttctgat ccttcatcat cttgatatgc
    28261 ggcagcctca gccacatttc ttccatcatg ctattcctgt gctgagaaca aagcaattat
    28321 gaaaacaaaa gggggttaag ccatcctaac ccctttgatt gtataattaa aatttttttt
    28381 gatgcttcca atctctgtcg ctaaactttt caggaacagt aatgatccta ttcacaaagt
    28441 cctcaatacg tattgtatct ataatcttat cttcatgcat aaccgttata tgaaattcca
    28501 tcggatacaa agtcaaggaa ataccaccag ttcgcataga acctcgctta aattgcactg
    28561 cttcatctga tgcttctgca actttaatta taccctcaga accgggaaca aaatcattct
    28621 gtttgagttt ttataaatgg aacgggaatc ctgtagctgg cacaatcagt aataatctcg
    28681 acagaaaatt tatcatcctc caattcgtca attctgtctt ccgtaatcgt tatggagaat
    28741 aaggccgggc ctttcgtttc tccaaagccc caagaacccg gtctttgcat caacaaaatt
    28801 caccagattg gaaactaata atgataccaa aaaacaagac aaacaaaaca gcagaagtaa
    28861 gttagaatag tggtatggaa aagagacgaa atttcacacc ggaagaaaaa gcaaagatag
    28921 taattgaggt tctgagggaa gaaaaaacac tgaatgagat tgctgctcaa tatgaggtgc
    28981 atccaaacca gctaagccgc tggaaggcag aattcataag taacgcagca agagtattca
    29041 gcaaggaaac agatgaagca gaaaaggcca aacagtccta tgaaaaggag aaggatgaac
    29101 tgttaaagca gatcgggcag ctgtcttatg aggttgcctg gcttaaaaaa aaatctggcc
    29161 ggttctaaat cccgagatga ccggatgaaa atgatagata aaggagatga aaagctcagt
    29221 attacaacac aggctggatt tttaagcata aatcgtacaa gcctttatta taaacccgtg
    29281 cctataagcg atgaagaata cctgatcaag cgtatcattg atgtggtcta cacggcgcac
    29341 ccagaatacg ggtacagaag gatgactgtt atactaaaca gagattacgg tatcttaatc
    29401 aacagaaaga gagtacgccg ctatatgaga gagatgggta tacacggctt ctgtcctggc
    29461 cctaacctta gcaagcgcct gcataaaaaa tatctctacc cttaccttct taggggttta
    29521 aacatcaacc atcccaaccg ggtatggcct gttgatgtaa catactgtag gatgaagcac
    29581 ggctttatgt atatggttgc gattatagat tggtattcca ggtatattgt tggttactca
    29641 ttatcaaata cgcttgagag gacatttatc atagaagctg tcaaaaatgc tataaaacag
    29701 catggagctc ctgagattat taacagtgac aagggttctc aatttacaag tgaggattat
    29761 ataaacctgc ttaaggataa taatatcaag atatcaatgg atggaaaagg tagagcatta
    29821 gataatcagc gcatagagcg atttttcagg aactataaat gggagaagct ataccttgaa
    29881 gattgtgaaa ctggacatca gctaaggaag attacgcaag agtatatgga acattataac
    29941 tacaagaggc ctcatcagtc tttggacgat ataacaccgg cagagttcta ctttggcatt
    30001 ctaaatcagt accctaaagc agtgtgagat gctatgaggc cctgccccaa accccgtcct
    30061 cgccggaagg caggctggca tgtaaaatgc ccgtaaacaa caggatttta tgcctatggc
    30121 tgtcaaaggt caagatgaac tcgctcatcg ctcgcccttg acatccccaa actgttgtac
    30181 taaaacgtgc agttttaaca acaacgacat tatgaatatg gttaaagtaa ataatttaca
    30241 taaagaaagg agacctaact aagactgcaa aaaattctgt attgacaagt ggggtcatta
    30301 tactccagca ttttaccctt ctgatgttat aaagcaatgg cagatctctt tttaatactt
    30361 catccacgac gtccttatgc attactttat gtgccattaa atcattttga gataaagcca
    30421 gcccaggaaa tccgcctact tgctgatatc gaataaagtg attttgaact agtgtgttaa
    30481 ttcaccagcc taatttaatt atagatagtg acaatttttt tatattatta tataatacat
    30541 aataaaatgt tgaaaattca gataatttga aagtttcgga agtgcagtta actataaagg
    30601 catctatgtg ttgaagcaac aataatagcc tatagggtgt gcttcggctt catctttcgt
    30661 tgaaaaagag aaaatacctg gccaaataag ccaagccgat ctataacaaa ggaggtgtca
    30721 tatgcagcca acaatgattg cattactgaa caacgccgct ctattattgc ttttgcccgt
    30781 aatctatcag ctaaccagtc ttataaatac aagaaacact cgatttaagc aaatagtaag
    30841 cgggatatta attgctttgt tatgtatcgc tattatgtca gtgccattta agttttacct
    30901 gggagtacaa tttgatacac ggtctattct gattagcgta acggcattag tatttggacc
    30961 gattcctacg attataacaa tagctgctgc tatcatctta agaatccttc aaggtggtgc
    31021 tggtgcattg ccgggtgttg ctgttattat atccagtgct attattggct tagtgtggcg
    31081 gctttggata cattctgcgg caacgaagct gcgctggtta aatgtttatg ctatgagcct
    31141 tttggtccat gctgccatga tagcttgtat gaacctttta ccctatccgg ccagtgtaaa
    31201 tgtaatccgt gaaatcactt tgccagtact tttaatatac ccggctgcaa cagtaccgat
    31261 ttatcttctg ctaacccaac agcaggtatt tttgcaggtg cgggaccaac ttaagcagtc
    31321 agaagaacgt tttcgactgt tgtttgataa agccccgctt ggatatgaat ctcttgacat
    31381 tgacgggaat ataattgacg taaatcaaca gtggcttaat acttccgggt tttcaaggga
    31441 tgaagttata ggaaagtggt ttggagattt tattcacccg gcgtataagg aagaatttct
    31501 tgagaagttt tcacaattag aagaaacaaa gcaaattcac agcgagtttg aaatgctgca
    31561 taagagcggg aaatctctat ttattgcagt tgatggtaat atagtttatg agcctaatgg
    31621 agattttaat gaaactttct gtattctcaa agacataacc aggcagaaga aggtggagac
    31681 agacctgcag tcaagcgaaa agaaataccg acgactatat gagacaatgg cccaaggtgt
    31741 tatttatcag gcggctgatg gaaggataat atcggcaaac cccgcagctg aaaaaatatt
    31801 aggtattccg ttagaccaaa tgttgggaat gtcgtctctt gatccgagat ggaggacaat
    31861 tcgcgaagac ggttcggaat tgtcgggaat ggatcatcct tctatgattg ctctacggac
    31921 aggtaagcca tatggcccgg ttatactggg tgtatatcag cctatgctca aggattatgt
    31981 ctggatttcc gtaaatgcca tcccattatt cgaagatgga gaagctgccc cttccaaggt
    32041 atatactact tttcaagata tcactgccga gctaaaagcc aatcagaatt atcagctttt
    32101 gttcaatgaa atggttgatg catttgcttt gcacgaaatt atttgtgacg accaaggaac
    32161 cccaattgat tataggtttc ttgctgtaaa cccggccttt gaaaagatga ctgggctgaa
    32221 agcagcagat attctgggaa agaatgtact tgaaatcctt ccgaatacgg aatcatactg
    32281 gattgaaacc tacggaaagg ttgcgctgac cggtgaatca gttagttttg aaaattatgc
    32341 ggccgcaacc gacaaatact tccgtgttat ggcctatcag ccggctccaa tgcaatttgc
    32401 atgtaccttc tcagatgaca caacccaagt acgcgcagag catgccttaa aagaaagcga
    32461 aaagaaatat agcagttaca tcgaaaatgc accctatgca gtttttgttg tcgataaaaa
    32521 aggacagtat gtagaagtaa acaacgccgc ctcaattata acaggatata gtcgagagca
    32581 gctgcttgaa atgactataa gagatattac agccgaagag tctatgaacg aggcgatgcg
    32641 ctgtttcact tcaatgcttg atacgggcaa actcagtaca gaattaaaat atttacataa
    32701 agacggctcg ataagatggt ggacggtcga tgcggtaaag atatcggaga atcggtttct
    32761 tggattttca attgatatta cttctaaaaa acaagcggaa gaagagctta catttcaaag
    32821 caggcatgat taccttaccg gcttatataa tcgaaggtac tacgaaactg aactgaaacg
    32881 tttggatata gaaggccaat tgcctctgtc tgtaataacc ggagatataa atggcttgaa
    32941 gttaataaat gatgcttttg gtcacgcaga aggagacaaa ataataatag aatctgcgat
    33001 gataatacaa ggctgctgcc ggcaaggtga catattagcc cgaaccggag gagatgagtt
    33061 cggaataatc atgcctagaa ctgacagtgt tacagcactt gaagtattgc agaacattca
    33121 aaccgcactg ataaaatttg attctgaaaa caatttcgaa atgtatcagc atagtgtatc
    33181 actgggttat gcaacaaaag aaaagattga agaggacatc atgtacatat taaaaactgc
    33241 ggaagatcat atgtaccagc gcaagctgct tgaggctaac agtgtacaca gtactattgt
    33301 tgcttcaata aaggctgcat tgtttgaaaa aagccatgaa acagaagaac atgccgaaag
    33361 gttagtagaa ctatccaagg cgataggtat ggcactgaat ttatcacaag tagagctgga
    33421 caaactcgaa ctgcttgcga cactgcatga tatcggaaaa gtgggaatca aggataatat
    33481 cctgacaaaa cccggtaaac ttagcgaatc agaatgggct gagattaaaa ggcatcccga
    33541 aatcggatac cggattgcca tgacttctcc ggaacttgtg cctattgcag aagggattct
    33601 ctgtcatcag gaatggtgga atggcagcgg ttatcccaaa ggcttatgcg gcgaaagcat
    33661 tcctctgctt tcgcgaatca ttgctgttgt tgatgcttat gacgcgatga caaatgacag
    33721 accatacaga agtgcaatgg aaccagcggc agctattgag atcatcaagg aaaatgcggg
    33781 tacacaattt gatccgaaaa ttgctgagat atttgctgat ttaatgtcca aagagtagaa
    33841 taacgtcgct ggacatgtgt ccaaacataa tgtacccttg tttcatcaca taaacacaaa
    33901 cgctgtattc atcgaataca gcgttattat gtaaatacat attttttggg cgagactgtc
    33961 cgaaaagtca gtcacagcaa cgcctgaggc actatgaaat atggtataat a
//
LOCUS       DAOQUR010000024        32447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1602_length_32447_cov_9.078816, whole genome shotgun sequence.
ACCESSION   DAOQUR010000024 DAOQUR010000000
VERSION     DAOQUR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 32447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32447
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_32447_cov_9.078816"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="ytxC"
                     /locus_tag="PKI50_06710"
     CDS             complement(<1..876)
                     /gene="ytxC"
                     /locus_tag="PKI50_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HNT03436.1"
                     /translation="MFLVAIGSLDDYEIIKSEIYRQFENAKLEGIDTVYEDYQLGNTL
                     FISCGIKDKLEEFKYILSDILAGVIIGHYESKLIRKIVKENFFYLNGKEQAIVIDNAC
                     KLLGEEKQAQPVGFYKADRKSIIRREILKYLDTEKELNIEGFINFRLSMYVNELNETV
                     ERALEIFVAEREYNEFIKLLRYFVEIQECKVDTVHLLQSGDGSYLLYDDKKNSISSEY
                     FDELRSEILDDTINYDDLLISTLITVSPNRILIHDIDEFKNKELIQTISNVFAERITI
                     CRSCDLCSSLQESSET"
     gene            complement(1099..1851)
                     /locus_tag="PKI50_06715"
     CDS             complement(1099..1851)
                     /locus_tag="PKI50_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03437.1"
                     /translation="MKFLKTSFIPDSDVSLAMVDYRISPKIEESLNKIGIECLKTVRC
                     PELYDAVDGHPDMLMFHIGGNRIVLAPNVYEKMAPIIEKKGFAVTKGATWLVRNYPGN
                     IAYNVLRIGKTAFHNIRHTDAEIIKALEKENVKMLNVNQGYTKCSVCILNDNTIITSD
                     EKISEIAEKNGIESFLTKPGGIDLKGLNYGFLGGASGLVSKNKIAFTGSLYTLEDNRK
                     LIDYINKKGFEIVHLSKGRLMDYGSILPLKCR"
     gene            complement(2012..2085)
                     /locus_tag="PKI50_06720"
     tRNA            complement(2012..2085)
                     /locus_tag="PKI50_06720"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2051..2053),aa:Cys,seq:gca)
     gene            complement(2094..2168)
                     /locus_tag="PKI50_06725"
     tRNA            complement(2094..2168)
                     /locus_tag="PKI50_06725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2134..2136),aa:Gly,seq:gcc)
     gene            complement(2175..2250)
                     /locus_tag="PKI50_06730"
     tRNA            complement(2175..2250)
                     /locus_tag="PKI50_06730"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2215..2217),aa:Phe,seq:gaa)
     gene            complement(2262..2338)
                     /locus_tag="PKI50_06735"
     tRNA            complement(2262..2338)
                     /locus_tag="PKI50_06735"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2302..2304),aa:Asp,seq:gtc)
     gene            complement(2551..2626)
                     /locus_tag="PKI50_06740"
     tRNA            complement(2551..2626)
                     /locus_tag="PKI50_06740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2591..2593),aa:Val,seq:tac)
     gene            complement(2753..3643)
                     /gene="hslO"
                     /locus_tag="PKI50_06745"
     CDS             complement(2753..3643)
                     /gene="hslO"
                     /locus_tag="PKI50_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNT03438.1"
                     /translation="MKDYVVRGASIDDSVRFFSCITTNMAEEGRKIHNCSPVSIAALG
                     RMLTAGSMMGIMLKSDEETITLQINGKGPAGSIVVISDSTGITRGYITNPDVELMHRE
                     NGKLDVGRAVGVDGVFTVIKDMGMKEPYVGQIPIVTGEIGDDISSYFAVSEQIPTAVG
                     LGVLVGIDCSVAAAGGFIIQLMPEADHDMITRLEDRLREIRSVTEMISSGLDAEGMIK
                     SILGDIEYKILEKKQVGYKCSCSRERMERALISIGRNDLEELIQEQGCAEIVCHFCNK
                     KYFFEKNELIEMFEKASSSI"
     gene            complement(3671..4414)
                     /locus_tag="PKI50_06750"
     CDS             complement(3671..4414)
                     /locus_tag="PKI50_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT03439.1"
                     /translation="MNCYNVLAELYDMFMEEIDYKAWCRYVEDIFKSCGIRPARVLDT
                     ACGTGNITIQMSKSGYNLWGLDISADMLTVAENKARAAKQKITFLNQDMTKMNLKEKF
                     DAVLCMCDGVNYINDEEALKNYFKLVYERLEDIGIFIFDISSYYKIKYIIGNNTFYEE
                     KNNKHYIWNNNFDEKSETVEMDLIFFVPEEGLYRKFSEYHVQKAYRSEYLARLLESTG
                     FEEVSCFDGFSFNKPEEKSERIFFAALKK"
     gene            complement(4411..5136)
                     /locus_tag="PKI50_06755"
     CDS             complement(4411..5136)
                     /locus_tag="PKI50_06755"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HNT03440.1"
                     /translation="MEIYKKAAELIKKCKPSVVLTGAGISTESGIPDFRSPGSGLWEK
                     IDPMEALSTEVLYKNPKKFYDLGFSIITSMKDSLPNKAHYLVAELEEKGLINLVVTQN
                     IDGLHHKAGSRNVYEVHGTVRTCSCDSCGELYDTGIIEKRIKAGEIPPKCRCRGIIRP
                     DVVLFGDMLPDCFYDSIKKVEEAELLIVIGSSLSVSPVNYLAEICKRLLIINLGETGY
                     DRKSDLIIRESTSSALERIVSFI"
     gene            complement(5209..5397)
                     /locus_tag="PKI50_06760"
     CDS             complement(5209..5397)
                     /locus_tag="PKI50_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HNT03441.1"
                     /translation="MKSKKTKKKQIILTPEDKMKYEIANELGLGEKLSQVGWGGLTAE
                     ETGRIGGLITRKKRHLPS"
     gene            complement(5476..6792)
                     /locus_tag="PKI50_06765"
     CDS             complement(5476..6792)
                     /locus_tag="PKI50_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03442.1"
                     /translation="MGKVIRLLVFTVLVMIMFAVAAYADSIDTGWKGYGLYNLNKLDV
                     TITNESDNITIDGEKVTAVYEYTIKSNADKDIAVNFGIPDNGIVKFSIYDGSKFLNYK
                     TRNTSYLKSTYGAENLQTPDGRWYLFTMVFTPGQTRSISINIEAQMTKEENDTYGLSF
                     FKDRGYSYAIKSEKTWITLNLSDFKPYDIYELEGVKEGQISDNGVATLSFSGDYGNGV
                     FLRYQPIDKMALDSLGKSTQKKPKSIVKAFNEKKYVEALNLCNEYISSPSDSSLNIEQ
                     VKFIKAECMRLSGNYAEYLSTVEQLDISQLYPGRIRFKILYDRLTAYKNTENAEGVNT
                     ILKELIPETQQIYPFLYHWLDRNGYKLLEAEPDAEDEASLNNQVSTENKGFDILGAVI
                     GFFAFVTESRWTYVLLGFIAGFIAGFIIGRITKRRKKSRSVYLFRD"
     gene            complement(6834..8000)
                     /locus_tag="PKI50_06770"
     CDS             complement(6834..8000)
                     /locus_tag="PKI50_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNT03443.1"
                     /translation="MLHIDDKVQALKDEVISIRRHLHRIPEKGLNEHETTGYIYEYLK
                     DLGINAEISPAGTGVIAYIEGKSGSKAIAFRADIDALSIEEQTGVEYSSENKGFMHAC
                     GHDGHTAVLLGFARLLVNMGNALDNNVLLVFQPAEEGPGGAEIIVKNGALEQYDASCI
                     FGLHIFPDIEEGKIGCRPGALMAQTGEFDLYIEGKSCHGAMPHKGNDALVAASNVILA
                     LNTIVSRFTDPLEPAVVTVGKMVSGERMNVIAGEALLQGTMRAFNDTAYNTLKDRVIK
                     MADSISGAYGCSCRYEIRDMYPAVHNDERLYKILAEDIGDENFVYMNPIMLAEDFSYY
                     QKRIPGLFFMLGAGNREKGYIHPLHSNMFNFNDEILITGVQIFYNLLRRCKQFQ"
     regulatory      8213..8405
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            8500..9492
                     /locus_tag="PKI50_06775"
     CDS             8500..9492
                     /locus_tag="PKI50_06775"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNT03444.1"
                     /translation="MNKYDVAVVGATGVVGRMFLKVLEERNFPIKNFYAFASAKSAGT
                     KITFSGKEYVVEELSEKSFDRHIDIALFSAGGETSKKYSPVAASKGVVVVDNSSAFRM
                     DPDVPLIVPEVNPHAVKNHSGIISNPNCSTIQAVVALKPLHDKYKIKRIVYSTYQSVS
                     GTGVKGVVDLEEGIKGNAPKIYPHPIAYNCLPHIDVFLDNGYTKEEMKMVDETKKIFD
                     DQSLKITATTVRVPVYYAHSESINAEFENDFDIDELKGLLAGSPGIIVQDDPANCIYP
                     LAINAAGRDEVFVGRIRRDYSVDYGVNLWVVADNIRKGAATNAVQIAELLISGK"
     gene            9518..10405
                     /gene="dapA"
                     /locus_tag="PKI50_06780"
     CDS             9518..10405
                     /gene="dapA"
                     /locus_tag="PKI50_06780"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNT03445.1"
                     /translation="MAIFKGSGVAIVTPFNDNGVDFNKLGELLDWHVENGTDAIIICG
                     TTGEAPTMSDDEKKTAYKFTVDKIAGRIPVIAGTGSNDTHHSIELSKYAESVGCNGVL
                     CVTPYYNKTTQKGLIAHYTAIADSINIPVIIYNVPGRTGINTNADTLKALSVHENIGG
                     IKEASGNISQVVEIGMFCDDNFCMYSGNDDQVVPLLSVGGIGVISVVANIAPKLMHDM
                     VDSFLKGDVKTAMKLQLDIKKLNDALFCEVNPIPIKTAMNLLGMNVGKLRLPLVDMSE
                     GNLNRLKNELINSGFELRG"
     gene            10411..11175
                     /gene="dapB"
                     /locus_tag="PKI50_06785"
     CDS             10411..11175
                     /gene="dapB"
                     /locus_tag="PKI50_06785"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNT03446.1"
                     /translation="MIKLMINGCNGKMGQVITRLAQGCDDLSIAAGVTRNPSRLPNSY
                     PVYRSFKEAEVKADVVVDFSNPEMIPETVDYCVKHHTALVVGTTGLSETDLKMLEKAS
                     ESIPVFVSANMSFGIRVLIDLALKAAATLGDEFDIEILEKHHNEKKDAPSGTAMMIAN
                     ELKSRINDGLEFVYDRSKVREKRKPNEIGISAIRGGTIPGEHTVFFAGKDEVIEIKHS
                     ALSRDMFGRGAIRAARYIASKQKGYYTMKTMLEELI"
     gene            11175..11891
                     /gene="dapD"
                     /locus_tag="PKI50_06790"
     CDS             11175..11891
                     /gene="dapD"
                     /locus_tag="PKI50_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HNT03447.1"
                     /translation="MNQKLDITNPYEIARYIKESKKVTPVKAYVNGDLKDADMSGIRA
                     YGADNFWILFGDHSDMEKFLSDNKSLIRSFELEYDRRNSAIPLLDTRYVDARIEPGAV
                     IRDKVLIHKNAVIMMGAVINIGAEVGENTMIDMNAVLGARAVIGSNCHIGAGSVIAGV
                     LEPPSADPVIIEDNVLIGANAVVLEGVCIGKGAVVAAGSVVTKNVEPDTVVAGIPARM
                     IKSVKDLNEDKKKLLDDLRG"
     gene            11905..13110
                     /locus_tag="PKI50_06795"
     CDS             11905..13110
                     /locus_tag="PKI50_06795"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNT03448.1"
                     /translation="MAIIVQKYGGTSVETIEKINNIAEHIVKRKSEGNDMVIVVSAMG
                     KTTDHLINMAHEISPDPDKRELDVLMATGEQVSMSLLSIALNNLDADAVSFTGQQVGI
                     RTNGSHTKSKIADINEEKIREEHRKGKIVIVAGFQGVNEYNEITTLGRGGSDTTAVAL
                     AAKLGAQCEIYTDVDGIYTIDPRLYPRAKKLDCISYEEMLEMASSGASVMHSRSIELA
                     EKYKVPVLVALSRGDIPGTIIKEMDNTMENTAITGLAVNNDEAIITLNGVPHDIKVIA
                     EIFQSIANKDINIDMISQTFPVNKLVSISFTLPKTDLYQASKVLNTFKKKLFTFTYET
                     CDNITKLSVVGLGMNTQSGVAAKVFNLLAENDIPVSIVTTSEIKISYVISPDDQKKAI
                     EAIAKEFDL"
     gene            complement(13214..14062)
                     /locus_tag="PKI50_06800"
     CDS             complement(13214..14062)
                     /locus_tag="PKI50_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNT03449.1"
                     /translation="MRKLRYVPILIMIVLGASLGGIAFNAFLIPHKLLSGGVSGVALI
                     LNYIFGLNPGVLIFIFNIPIFLAGYKFMEKEFILLSLVGMTVFSVSIDLFSFLKNVVY
                     IEDTLLSCLYGGVLNGIGMGLVFRNRASQGGVDIVAVIVKKYFSMNLGKTSFLINIIV
                     IGIASLFFGLEPAMYTLVSMYVASTVLDKVQQGFGSSKSVMIITDNEQQVADAILKTL
                     DRGVTYLEGEGAYTGNKKRVIYCIVALKQLAKLKQIVNEIDSSAFMAVSDTAEVLGYG
                     FGNRGI"
     gene            complement(14059..14595)
                     /locus_tag="PKI50_06805"
     CDS             complement(14059..14595)
                     /locus_tag="PKI50_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNT03450.1"
                     /translation="MKLEKGLVQVYMGNGKGKTTAALGQGLRSCGRGLRVCMVQFLKG
                     GDTGELHSVEKLYPLFEIFRFERERGFFWTLSEQEKRELKKDIDKGFEFIKNMLRENS
                     CDVLIIDELLGVLENKLLEVNDVLELLKSKPVNMEVIITGRKAPKEIIDAADLVTEMK
                     EIKHYFNSGIPAREGIES"
     gene            complement(14713..15381)
                     /locus_tag="PKI50_06810"
     CDS             complement(14713..15381)
                     /locus_tag="PKI50_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNT03451.1"
                     /translation="MTDKVIESNNVTVYGTVTSKPEFSHEMYGEGFYTVYLEVPRLSD
                     ISDTLPITISERLVQGLNIDVGSTLLVEGQLRSYNKYHEGNNKLILTVFARNLKTMDQ
                     DVKNPNQIYLDGYICKKPVYRTTPFGREITDMLLAVNRPYNKSDYIPCIAWGRNARYS
                     ENLKVGDRIRIWGRIQSRDYQKKVSEDEVITRTAYEVSISKMEVIEGETGEKKDNSEE
                     EPEE"
     gene            complement(15566..16879)
                     /locus_tag="PKI50_06815"
     CDS             complement(15566..16879)
                     /locus_tag="PKI50_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNT03452.1"
                     /translation="MNVDSHEGIGVTADPQKVRPGMIYVDLSCRRNRRRIYEAYEKGA
                     YMIFTPYNISDPALPVIKVESPRDTLCMLFEKFFEKQKSNAKLIGVFGESDKSILVGM
                     LQSIFSGKDNSRFDMDIIPITINTNENNLFYLDSMSFDSAILTDRCALGKCCGNKPAA
                     DFVSGLTEVRAVIMNNDEYYEINVSENCKRAGLITYGLNKKAVATASSIDVDEITCFN
                     YCVQRSFLSGSGARIEPFEIPVRLNVLGSHSIYNALAAITCGLYYDLDISDIKESIES
                     YKGSARHFQKIYEDGFTVIDNYCNSAYDYTAAFDSIQILSYENLVLVISVSQDVNMNF
                     HEEKAGLIAEWVRILKCKEVILTSCMDGDSHIGTLPLKSMKLYKRIFRENDISFRYYH
                     LLHHAIEKGLSLVNRNDLLVLLGSEEMDAASRIVYKLLGPMKKKN"
     gene            complement(17039..17224)
                     /locus_tag="PKI50_06820"
     CDS             complement(17039..17224)
                     /locus_tag="PKI50_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03453.1"
                     /translation="MMDRRFIKKLRKVLVLEAFFHGASSLSKVLDATESDLADIMKYF
                     DKKGSKKELPVGKPNKK"
     gene            complement(17275..18432)
                     /locus_tag="PKI50_06825"
     CDS             complement(17275..18432)
                     /locus_tag="PKI50_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNT03454.1"
                     /translation="MSKIELVAPCNFAVEAILSREIKSLGYEVTKVEDGRVSFMADEE
                     GICRSNLWLRTAERILVKIDEFEALSFEELFEKTKRLQWNRWIPKDAEFPVAKASSIK
                     SKLFSTSDIQSIVKKAVVESLKTKYKQQWFEETGERYPIHVFLNRDKASLYLDTSGLS
                     LHKRGYRELSNAAPIKETLAAAMVLLTPWKADRAFIDPLCGSGTIPIEAAMIGLNIAP
                     GRGRSFVSDNWRWIGSKLWNRAKEEAGALERRDDVLNIQGYDIDESALKVARINAKMA
                     GLEDKLHFQKRDISELSSKEKYGFIVTNPPYGERLSDSKSVEGLYKEMGKVFSRLETW
                     SFYIITSHEGFERFFGRRADKKRKLYNGMMKTDLYQFFGPKPPRKVEKIDE"
     gene            complement(18511..19281)
                     /gene="pdaB"
                     /locus_tag="PKI50_06830"
     CDS             complement(18511..19281)
                     /gene="pdaB"
                     /locus_tag="PKI50_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family sporulation
                     protein PdaB"
                     /protein_id="HNT03455.1"
                     /translation="MNFRFAKNKYILNIMLVVIVVSVSLIYNYAGYKQILNVFRNNNR
                     ELPIYCVDTPEKKVAISFDAAWGADYTESLLKILKNYDVKTTFFLVGFWVDKYPEMVK
                     RIDEEGHEIGNHSSRHPHMSQLSKEQIIDELAKTSEKIEAITHKKVTLFRPPFGDYNN
                     RVIETSRAMGIQVIQWDVDSLDYKDYGADAIVKRVLSKVRNGSIVLFHNNAAYTADAL
                     PIILENLQKEGYKIVPVSELIYKENYYIDHTGMQKLLR"
     gene            complement(19940..20509)
                     /locus_tag="PKI50_06835"
     CDS             complement(19940..20509)
                     /locus_tag="PKI50_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT03456.1"
                     /translation="MLLSNSLAISHEIIKKAVKQGDIVVDATMGNGNDTLLLAGIVGV
                     EGKVYSFDIQDTALINTAKKLREAGVSDIVELICDGHENIDAYVPKGIKAVMFNLGYL
                     PKGDHSIGTKACTTIEALKKSMEMLTDGGIITVVIYYGGDSGFEEKEAVMEYIRTIDC
                     RKYSVLVCDFVNQINCPPIAVCIEKIGRQ"
     gene            complement(20528..20908)
                     /locus_tag="PKI50_06840"
     CDS             complement(20528..20908)
                     /locus_tag="PKI50_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNT03457.1"
                     /translation="MGSNKILGAFGENKACEYLEECGYRVLERNFACRTGEIDIIALE
                     GEIIAFIEVKTRSSERYGLPAEAISRNKQNKLVTAALYYMQRNGYQNRMCRFDVMEII
                     IDGEDNYRINLIKDAFQYSGRYGY"
     gene            complement(20895..21176)
                     /locus_tag="PKI50_06845"
     CDS             complement(20895..21176)
                     /locus_tag="PKI50_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNT03458.1"
                     /translation="MDKKLKKAAALTYEAGDDAPRVTALGKGEIANRIIETAKKNNVP
                     IFEDSGLVDALLQLDIGGQIPQELYSVVAEVLVFVSKIDKLKGEKYGQQ"
     gene            complement(21178..22872)
                     /locus_tag="PKI50_06850"
     CDS             complement(21178..22872)
                     /locus_tag="PKI50_06850"
                     /inference="COORDINATES: protein motif:HMM:NF040034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNT03459.1"
                     /translation="MKIDASSFRLIKPELIELLKNLETGDVLKGKVLEALGNSILIRA
                     KSGEIFTAILQEGASIPKDAFVELVIKDIADGKIFAELKGEDKASDPDIKVSDLLKQL
                     NLPVNEKNMEAAKLLLKYKLPLNRETIGNITGLQKSIDNLNNSTGEKMGLMLSGLNIK
                     DTPVDVLNKIVLNWPDDFAAFEAILENDGQKPEANEAAIIKQTTAGSASDSKAAEIPG
                     KAAVITITDSSEALKGAVITDSNETPREAAITGRGETPKEGVGYVSPLSDMAAAESNK
                     GVELIRALAGLGIESESEIKMLAAKVESILSSIKNTDMEAITYLVSKEMEITPRNLGM
                     LIKNIENRDVISQFLDKLQEKIIRDDNPKLKAISESIRKVFLEPRQLEDREEVARQLK
                     DIASLGEKLESYLKTGGDKDPEIREALSNLKDCIDFIRNVNQHNNFLQIPLLINDNTT
                     AAKLYVFKDGKREKRINPEDATVVVALDLMNLGHLESMIRVKGKTVDITFRTENKSIG
                     SMIEKNILMLKEALGEKGYYLSPIRVINLEQPFSLLSLEAMINESGYEKIHFDMRV"
     gene            complement(22878..23522)
                     /locus_tag="PKI50_06855"
     CDS             complement(22878..23522)
                     /locus_tag="PKI50_06855"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNT03460.1"
                     /translation="MAVRIKYDDKEKERLIRMTEYERKCYEKGYKLVAGVDEVGRGPL
                     AGPVVAAAVILGEGVLIPGVNDSKKLSEEKREYLYDEIKSKALCCSIGIVDEKTIDEI
                     NILNAACLAMKKALDGLAERPDYILLDAVTLKDMDIPQKGIIKGDTLSLSIAAASIIA
                     KVERDRMISAYDELYPQYAFSKHKGYGTREHIECIKKYGLLPIHRRSFTKNFQG"
     gene            complement(23948..24085)
                     /locus_tag="PKI50_06860"
     CDS             complement(23948..24085)
                     /locus_tag="PKI50_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HNT03461.1"
                     /translation="MRSFEWILCPVCQSKTRIRILPATVLKNFPLFYPKRKQKTLVNI
                     K"
     gene            complement(24117..24551)
                     /locus_tag="PKI50_06865"
     CDS             complement(24117..24551)
                     /locus_tag="PKI50_06865"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HNT03462.1"
                     /translation="MKKLLVKIEFGVAIGCFGFVSMLFIASAFAGGANAFIGQKTGEE
                     MLRLAACFIIIAIGFSVPSLVYEKENLTNSLKVLIHMAAGTVVYLITAFFAGWIEKSF
                     GAIVVYILIALGAAAIIWAIFMLAFKLQADKINRKIREKHED"
     gene            complement(24565..25005)
                     /locus_tag="PKI50_06870"
     CDS             complement(24565..25005)
                     /locus_tag="PKI50_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNT03463.1"
                     /translation="MKVEIKIYAEVIEPYAVIYTNEITAEISRIASFIEDSADNSIIT
                     VTDNERMFVLRPEEVYMLRVENEKTVVYTKVKKYNSNKRLYEFETILGSGFIRISKSV
                     LVNLKYLDCVEPSFGGIMLLVLKNGCKDYISRMYLPAFKKYLGI"
     gene            complement(25275..25469)
                     /locus_tag="PKI50_06875"
     CDS             complement(25275..25469)
                     /locus_tag="PKI50_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT03464.1"
                     /translation="MKNNIRNLRKDKGLRQEDLAQILGVTRQTIIAIENDKYNPTLEL
                     AMKLARFLESSVEDIFILED"
     gene            complement(25459..25698)
                     /locus_tag="PKI50_06880"
     CDS             complement(25459..25698)
                     /locus_tag="PKI50_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03465.1"
                     /translation="MKNQKWKADEMEIAINYRAMRIAFVFSIIALLAYCIYEYVALKR
                     LPTIPFVIVCVQEVLFFISKVFITSKMTKESDDEE"
     gene            25923..26320
                     /locus_tag="PKI50_06885"
                     /pseudo
     CDS             25923..26320
                     /locus_tag="PKI50_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            26295..27047
                     /locus_tag="PKI50_06890"
                     /pseudo
     CDS             26295..27047
                     /locus_tag="PKI50_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902276.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            27044..27709
                     /locus_tag="PKI50_06895"
     CDS             27044..27709
                     /locus_tag="PKI50_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HNT03466.1"
                     /translation="MIAKMIALDWRAMKYYQIRLLLLPVFVFIFGVYSPLTVIPMSVI
                     LSLSFSLNPFAVEEKGELNNLYLTLPVRRKSIVAGRYILSIIMLLCGIAIGILIMPLA
                     NKLSISKWFIGIEEFIVVISLSYLLYAILNLFMFPMLFRLGYHKGKFWGFYLPMVFFA
                     LLFGAYLAITHLPGNKTLTLNFIVYASENLLMVSGSIVVLATMLLILSFMISLKLYSK
                     RDF"
     gene            complement(27767..28261)
                     /locus_tag="PKI50_06900"
     CDS             complement(27767..28261)
                     /locus_tag="PKI50_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03467.1"
                     /translation="MKKQNSQDERVVAQRRKINSEAYGILMIALLCSMLVQQFLLNAP
                     FEQYAVEFICFFGMSLYLMIRHLTLGLNIYGEGKRAKTIPIINGIVTGIVVTTITGVS
                     NYTQYAEHYKEDGIGYFIAVLAITFISATVFSFVVLYCFHYLNKKKQARIQKQLDEKE
                     QDEL"
     gene            complement(28258..28458)
                     /locus_tag="PKI50_06905"
     CDS             complement(28258..28458)
                     /locus_tag="PKI50_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT03468.1"
                     /translation="MKNKRMKIARMEVDMKQEDLAKAVGVTRQTISLIESGEYNPTLN
                     LCIAICKELSKTLNDLFWEESK"
     gene            complement(29289..29594)
                     /locus_tag="PKI50_06910"
     CDS             complement(29289..29594)
                     /locus_tag="PKI50_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT03469.1"
                     /translation="MPDFGQPFSGLKKEHKISHQELIRSIRFMIAAEYEAIQLYMQLA
                     ESTDNKLAIEVLKDIADEERVHAGEFLRLLKELDPDEEKFYAEGAEEVEEEIEKLKK"
     gene            complement(29618..30778)
                     /locus_tag="PKI50_06915"
     CDS             complement(29618..30778)
                     /locus_tag="PKI50_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT03470.1"
                     /translation="MPKKYAVRNVRLCTKDCLCLYVCPTGATDTENSIIDVAKCIGCG
                     DCADACPSGAISMVPVEYPTQQSKNETVVNSLNVLLRSKSEQENIAAGLSGKLAIAIE
                     KSNHIMAEDIIREAGYMLPESENARAFLNSLLDKKQSEDFPIEVVKKLLDSFKTNNIK
                     LQEDKKMVKYRCSVCGYIHEGELPEGFTCPRCKQPASVFVKVEEKTNSGNQYVGTKTE
                     KNLMEAFAGESQARNKYTYFANIAQREGYDQLSEIFLKTARNEQEHARIWYEELGNLG
                     KTAENLLHAAEGENYEWTDMYDRFAKDAEEEGFKDLAERFRNVAAIEKAHEERYRALL
                     KNVEMQQVFEKGEEIMWECRVCGHLVIGKKAPEVCPVCKFAQSYFEVRKENY"
     gene            complement(30781..31452)
                     /locus_tag="PKI50_06920"
     CDS             complement(30781..31452)
                     /locus_tag="PKI50_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNT03471.1"
                     /translation="MAKYVCSVCGYAYDEAKGIPEAGITPGTAWVDLPEDWICPLCGA
                     TKPEFEKQGEIASTEVKKPTFVIEPSTDIKEMSALEISALCTNLARGCEKQYKLEEAA
                     LFNELAGYFKKASAPAESPDIDQMIALIEKDLEEGFPNANALAADVKDRGALRALVWS
                     EKVTRILKSLLTRYKKEGDAMLENTGVYVCTICGFIYIGDNLPDVCPVCKVPNWKFEK
                     IEGRS"
     gene            complement(31466..31897)
                     /locus_tag="PKI50_06925"
     CDS             complement(31466..31897)
                     /locus_tag="PKI50_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNT03472.1"
                     /translation="MKSSFEELKEKLKMTNIYLSHQRLKVLEYLVQNHCHPTVDQIFT
                     DLQKDIPTLSKTTVYNTLRILVKSGLVRVITIEDNETRYDIVTENHGHFKCESCGTIF
                     DFPIDIDSLVTGDLSKFKINDKNVYFKGLCPRCLSNINEIK"
BASE COUNT         9013 a         7346 c         5742 g        10346 t
ORIGIN      
        1 tgtttcgctg ctttcctgca gactgctgca caaatcgcag ctgcggcata tggttattct
       61 ttccgcaaac acattggata tggtttgaat cagttcctta ttcttaaact catctatatc
      121 atgtatcaat attctattcg gagaaaccgt gattaatgtg cttatcaaca gatcgtcata
      181 attgattgtg tcgtccagta tttccgaacg cagctcatca aagtattcgc tgcttatgct
      241 gtttttctta tcatcgtaca acagataact gccatccccg gattgaagca aatgaacagt
      301 atctaccttg cattcctgta tttcaacaaa atatctcagc agttttatga actcgttata
      361 ttccctttcg gcgacaaaaa tctccaatgc tctttcaact gtttcattaa gttcattcac
      421 atacatactg aggcggaaat ttataaatcc ttctatattc aattcttttt cggtatccag
      481 gtatttaagt atttctctcc tgataatact ttttctgtcg gctttataga atcctaccgg
      541 ctgagcctgc ttttcctcac caaggagctt gcatgcatta tcgataacaa ttgcttgttc
      601 ttttccattc agataaaaga agttttcttt tacaattttc ctgataagct ttgattcata
      661 atggcctata atcactcctg caagaatatc tgaaagaatg tatttgaatt cctcaagctt
      721 gtcttttatg ccgcagctta tgaaaagtgt atttccaagc tgataatcct cataaactgt
      781 atcaatgcct tccagtttcg cattttcaaa ctgtctgtat atttcagatt ttataatttc
      841 ataatcatcc aaggaaccaa ttgctacaag aaacatttgt tctgctccct ccttgccgga
      901 tactgcaaat aattaattgt acggagagta tccaaaaaaa tttcaggaat cattaaatcg
      961 tctttcaatg attagtattt gcctaaatat ttcttgtatt caaagccaag tgacaggtta
     1021 attttttcaa aacgctgccg cactttattt tcatgagttt tgtgtactat cattatactt
     1081 ggtctgtaaa tgtttcaatt aacggcattt caaaggcaga attgaaccat aatccataag
     1141 tcttcccttt gacaaatgca caatttcaaa acctttttta tttatataat caataagttt
     1201 tctattatct tcaagagtat aaaggcttcc tgtaaatgct attttatttt tggatactaa
     1261 gccgctggca cctccaagga agccataatt caatccttta agatcaattc ctccgggctt
     1321 tgttaaaaag ctttctattc cgtttttttc tgcgatctcc gatatttttt catcagaagt
     1381 aataattgta ttgtcattta aaatacatac cgagcatttc gtatacccct gatttacatt
     1441 cagcatttta acattttcct tttccagagc ctttattatt tccgcatcgg tatgccgtat
     1501 attatgaaat gccgttttcc cgattctcaa tacattatat gcgatattgc ccggatagtt
     1561 tctaaccagc caagttgcac cctttgtcac tgcaaatcct tttttctcaa ttattggcgc
     1621 catcttttca taaacattgg gagccagtac tatcctgttt cctcctatat ggaacatcag
     1681 catatccggg tgcccgtcca ccgcatcata cagttcaggg catctcactg tctttaagca
     1741 ttctattcct attttattaa ggctttcttc aattttaggg cttattctgt aatccaccat
     1801 tgcaaggctt acgtcagaat cgggtataaa ggaagttttt aaaaatttca tatgtaccag
     1861 ctcttttctg aataaaatat atggagaatg tgaaatactc cgactgttgg ggaagtgtgt
     1921 cctccccacg tcggtatttg catttctcca cgcccttaga cacatataaa aacaaaaaaa
     1981 cttattgtat tcaataagtt gaagttaaaa atggaggcga cacccggact tgaaccgggg
     2041 gtaaaggatt tgcagtcctc tgccttacca cttggctatg tcgccataaa atttggagcg
     2101 ggttacgaga ttcgaactcg cgactttcac cttggcaagg tgacactcta ccactgagtt
     2161 aaacccgcat tgtatggtgc cttggggcgg aatcgaacca ccgacacgag gattttcagt
     2221 cctctgctct accgactgag ctaccaaggc ataatattaa atggcgaccc agaacgggct
     2281 cgaaccgtcg acctccagcg tgacaggctg gcattctaac caactgaact actgggccac
     2341 ttattacctc cgcaagtgct acgcattaaa tcaaggaatg tcggtctttt tccgcgaagt
     2401 agtttggtta aagctgctcc gcaaatgcta cgcattaaat caaggaatgt cggtcttttt
     2461 aattttgcgc tatgcgggtc ataaattgct ccgcaagtgc tacgcattaa atcaaggaat
     2521 gtcggtcttt ttccgcgaag cggaaaaagt tggtggggac aatagggctc gaacctatga
     2581 ccctctgctt gtaaggcaga tgctctccca gctgagctat gcccccgctg aaacttgcga
     2641 catcatttag tataacctac agataatcta aagtcaaaac ttaatttttt gtatttctta
     2701 catttatcat tttattgcac tgaattcctt tgataatctt tagcatacta tattatatac
     2761 ttgatgatgc tttttcaaac atctcaatca gctcattctt ttcaaaaaaa tactttttat
     2821 tgcagaaatg acatacgatt tccgcgcatc cttgctcttg tataagctcc tccaaatcat
     2881 tcctgccaat gcttatcagc gccctttcca tcctttccct gctgcagctg catttgtagc
     2941 ctacttgttt cttttccaat atcttatatt caatatcgcc taaaatgctc tttatcatac
     3001 cttcggcatc caaccccgaa ctgatcattt ccgttaccga gcgtatttcc ctgagccggt
     3061 cttcaagcct tgttatcata tcatggtctg cttccggcat aagctggatt atgaatcctc
     3121 ctgcagcggc tacgctgcag tctatcccta ccagaactcc caggccaacg gcagttggta
     3181 tctgttcgga tacggcaaag taagaggata tatcatcgcc tatttctcct gttacaatcg
     3241 gtatttgccc cacatagggc tctttcattc ccatatcttt aataacagta aagacgccgt
     3301 caacacccac tgcccttccc acgtcaagct tgccgttctc ccgatgcatt aattcaacat
     3361 ccggatttgt gatatagccc cttgtaattc ctgtgctatc ggatatgact actatgcttc
     3421 cggccggccc tttgccattt atctgcagag ttatggtttc ttcatccgat ttaagcataa
     3481 ttcccatcat acttcccgca gtcagcattc ttcccaaagc tgcaattgaa accggactgc
     3541 agttatggat ttttcttccc tcttccgcca tattcgtggt aatacagctg aaaaacctta
     3601 cgctgtcgtc aatacttgct cctcttacaa cataatcctt cattatttcc tccagttatc
     3661 attcaacatt ctatttcttt aaagcagcaa aaaagattct ttcgcttttc tcctcaggct
     3721 tgttaaagct gaaaccgtcg aagcagctta cttcttcaaa gcctgtactt tccagcagcc
     3781 tggccaggta ttcgctcctg taagctttct gtacatgata ttcactgaac tttctgtaga
     3841 gcccttcctc gggaacaaaa aatatcaggt ccatttccac agtctcagat ttttcatcaa
     3901 aattattatt ccaaatataa tgcttattat ttttttcttc gtaaaaggta ttattaccaa
     3961 ttatgtattt gattttataa taactgctta tatcaaaaat gaaaatccct atgtcttcaa
     4021 gcctttcata aaccagctta aaataatttt tcaaggcttc ctcatcatta atataattaa
     4081 cgccgtcaca catgcaaagc actgcgtcga atttttcctt taaattcatc tttgtcatat
     4141 cctgattcaa aaatgttatt ttctgcttcg ctgctcttgc cttgttctcc gcaacagtca
     4201 gcatatcggc cgatatgtca agcccccata gattataacc ggattttgac atttggattg
     4261 taatatttcc cgtgccgcag gcagtatcaa gtactcttgc cggtctgatc ccgcagcttt
     4321 taaaaatgtc ctccacatat ctgcaccagg ctttatagtc aatttcctcc ataaacatat
     4381 cataaagctc agccagcaca ttataacagt tcatatgaag ctcacaatcc tttccaaggc
     4441 ggaagatgtg ctttccctta ttattaaatc gctcttcctg tcataaccag tctctcccag
     4501 gtttattatc aacagccttt tgcatatttc cgcaagatag tttacgggtg acaccgagag
     4561 gctggatccg atcactatca gaagttccgc ctcttcaacc ttcttaatgc tgtcgtaaaa
     4621 gcagtcgggg agcatgtccc cgaaaagcac aacatcaggc cgtattatcc ctctgcatct
     4681 gcattttggc ggaatctctc ccgcttttat ccgtttttct attatgccgg tatcatatag
     4741 ttctccgcag ctgtcacagc tgcaggtcct cacggttcca tgcacctcgt atacatttct
     4801 gctgcctgcc ttatgatgca atccgtcaat attctgcgtc actacaaggt taatcagccc
     4861 tttctcctcc agttccgcaa ccagataatg agccttattc ggaaggctat ccttcatgga
     4921 agttataatg ctaaaaccta aatcatagaa tttttttggg tttttataca atacctcggt
     4981 tgacagagcc tccatggggt ctatcttctc ccacagtccg ctgccggggc ttctgaaatc
     5041 aggaataccg ctttccgtcg aaattccggc tcctgtcaac actactgaag gcttgcattt
     5101 ttttattaat tctgcggctt ttttataaat ctccatcgga tacccatcct ttcatcgatt
     5161 gcgcttaaga gcaacagccg catcatgcgg ctgtaattat gctaatgatc agcttggcaa
     5221 gtgcctcttc ttcctggtaa taagtccgcc tatcctaccg gtctcttctg ccgtcaggcc
     5281 tccccatccg acttgagaca gcttctctcc aaggccaagt tcatttgcta tttcatattt
     5341 cattttatcc tcaggggtca gtatgatttg ttttttcttt gtcttttttg acttcataat
     5401 atcacttcct caagaattgt tttttagtgc attggatagc aatattatgt gaattttttc
     5461 ttgcattatt ctgttttaat ccctaaaaag ataaacagat ctgcttttct tccttctttt
     5521 tgtaatcctt ccgataatga agccggcaat gaagccggca ataaagccta ataatacata
     5581 tgtccatctg ctttcagtaa caaaagcgaa aaaaccaatg accgccccga gtatatcaaa
     5641 acccttattt tcagtgctta cctgattatt cagggatgct tcatcttctg catccggctc
     5701 ggcttctaaa agcttgtacc cgtttctgtc aagccaatga taaagaaaag gatagatttg
     5761 ctgagtttca ggaatcagct ctttaagtat agtattcacc ccttcggcat tttctgtatt
     5821 tttatatgct gtcagcctgt catacagtat tttgaaccgt atccttccgg ggtaaagctg
     5881 agatatgtca agctgctcca ctgtgctcaa atactctgca taattacctg acaacctcat
     5941 acattctgct tttataaact ttacctgttc aatattcagg ctgctgtcag agggtgaact
     6001 tatgtattca ttgcaaagat taagcgcttc cacgtatttt ttttcattaa aggctttgac
     6061 tatggatttc ggcttttttt gcgtactttt cccaaggctg tccaaagcca ttttgtcaat
     6121 gggctggtat ctcaggaata ctccgttccc atagtctccg ctgaaggaaa gtgtagcaac
     6181 cccattatcg gatatttgcc cttcctttac tccttcaagt tcatatatgt catatggctt
     6241 aaaatctgac aaattcaaag ttatccatgt tttctcactt tttatcgcgt aggaatagcc
     6301 tctatccttg aagaagctca acccgtatgt atcattttcc tcttttgtca tttgagcttc
     6361 aatgtttatg cttatagacc gggtttgccc gggtgtaaat accatagtga ataaatacca
     6421 tctgccgtca ggggtctgaa gattctctgc tccgtaggta cttttgaggt atgaagtatt
     6481 tctggtcttg tagttcagga atttgcttcc gtcatatatg ctgaacttga caattccatt
     6541 atccggtata ccgaaattaa ctgctatatc cttatcggca ttggacttta tggtgtactc
     6601 gtatacggcg gttacttttt ccccgtctat tgtaatattg tctgactcat tggtaatagt
     6661 gacatccaac ttgttaaggt tataaagccc atagcccttc cagcccgtgt caatactgtc
     6721 tgcatatgca gccactgcaa acattatcat taccagaaca gtaaaaacaa gtaatcttat
     6781 taccttaccc attacccaac ctccttttct tcatatgtgc tgcatcccat aaattattgg
     6841 aattgcttgc atcttctcaa aagattataa aaaatctgta ctcccgtaat taatatttca
     6901 tcattgaaat tgaacatgtt gctgtgaaga ggatgtatat agcccttctc cctgttgcct
     6961 gcccccagca taaaaaacaa accgggtatc cttttctgat aatatgaaaa atcctctgca
     7021 agcattatcg gattcatata tacaaaattt tcatctccta tatcctccgc aaggattttg
     7081 taaagccttt catcattgtg aacagcggga tacatatcac gtatttcata tctgcagctg
     7141 catccgtatg ctccgcttat agaatcagcc atttttataa cacggtcttt taaagtatta
     7201 taggccgtat cgttgaaggc tcgcatggtt ccctgcagta atgcttcacc tgcgattaca
     7261 ttcatccttt cgccgctgac cattttcccc actgttacaa ctgcaggctc caaaggatcc
     7321 gtaaatctgc tgactatggt attaagggcc aagataacat ttgaagccgc aacaagggca
     7381 tcattccctt tatggggcat tgctccgtga cagctcttgc cttctatgta cagatcgaat
     7441 tcccctgtct gtgccattag tgctccgggc cggcaaccta tcttgccttc ttctatatcc
     7501 ggaaatatgt gtaacccgaa tatgcagctt gcgtcatatt gttccagcgc cccgtttttt
     7561 acaattattt ccgctcctcc ggggccttct tccgcaggct ggaataccag cagcacatta
     7621 ttgtccaatg cgttacccat gttaaccaat agtctggcaa aaccaagcag cactgcagta
     7681 tggccgtcat ggccgcaggc atgcataaag cctttatttt ctgaagaata ttcaactccg
     7741 gtctgttctt caattgataa agcatctata tccgctctga aagctatggc ctttgacccg
     7801 gacttgcctt caatgtatgc tattaccccg gttcctgccg gacttatttc tgcgtttatt
     7861 cccaaatcct tcaaatattc atatatgtac ccggtggttt catgctcatt gagccctttt
     7921 tccggaatcc tgtgcagatg ccgtcttata gagataacct catccttaag tgcttgtacc
     7981 ttatcatcaa tatgcagcaa aatatcactc cttgctgagt actttccagt tattcatata
     8041 atatattgta tattatatgg ttctgcattt caaatttttg tctttcgatg ttaagcagta
     8101 tgaatactat tgtaaccgtt atcatagcaa taaataaagc aaaaattaat attagctgtt
     8161 gacacgtccc aaaccctatg atagtattat tctaatttaa gaaaagtttc tattctagga
     8221 tagaggcgcg cttttaaata gtatgggtat tcaaaattgg gatgtggctt cccgaatctc
     8281 cggaaaggta aaagcgccga aggcggaaat atgccacgga tttctgacct ggtcccggct
     8341 caaaaagggt ccggactgtc accggagagt ctatgcacaa cctccggtgg agagctatac
     8401 atgaataagt gtgtttgttc agttttgttc tgaataaata tctttttcgt gtcagctttt
     8461 ttaccgggct ggcactttaa ttttaaagga ggcaattaga tgaacaagta tgatgtagca
     8521 gttgttggtg caaccggcgt ggtcggaaga atgtttctca aggtgctgga agagcgaaac
     8581 ttcccaatta aaaactttta tgcttttgcc agtgcaaagt ctgcaggcac caaaatcact
     8641 ttcagcggaa aggaatatgt ggttgaagag cttagcgaaa aatccttcga caggcacata
     8701 gacattgcgc tgttctcggc aggaggggaa accagcaaaa aatattcccc cgttgcagct
     8761 tcaaaaggtg ttgtcgttgt ggataacagc agtgctttca gaatggaccc cgatgttccc
     8821 cttatagttc cggaagtgaa ccctcatgcg gtaaaaaatc acagcggtat aatttcaaac
     8881 cctaattgtt caacaataca agctgttgtt gccttaaagc cgcttcatga caaatataaa
     8941 ataaaaagaa ttgtatattc cacataccag tcagtctcag ggacaggtgt aaaaggagtc
     9001 gtcgatctgg aggagggcat aaaagggaat gctccaaaaa tatatcccca tcccatcgcc
     9061 tataactgtc tccctcatat agacgtgttc ctggataatg gatatactaa ggaagaaatg
     9121 aaaatggtcg atgaaaccaa aaagatattt gatgaccaaa gcttgaaaat tacagcgaca
     9181 actgtccggg tacccgttta ttacgcacac tcggaatcaa taaatgcgga atttgaaaat
     9241 gactttgata ttgacgagct taaaggactg cttgcaggca gcccgggaat aattgtgcag
     9301 gatgatccgg caaactgcat ctaccccctt gcaattaatg cagcaggaag agatgaagtt
     9361 tttgtcggaa gaataagaag ggattattcc gtagattatg gagtaaacct ctgggtggtt
     9421 gcagataata tccgaaaggg tgcggccaca aacgcagtac agatagctga gctgttaatc
     9481 agcggcaaat aatatataaa tgtgaggagg atttggaatg gctatattca aaggttcagg
     9541 ggttgcaatc gttacccctt tcaatgacaa tggagtagat ttcaacaaac ttggagaatt
     9601 attggattgg catgttgaaa acggtacaga tgcaataatt atatgcggca ccaccggtga
     9661 agcacccaca atgtcggatg atgagaaaaa aacggcatat aaattcacag tggacaaaat
     9721 tgccggaagg attccggtta ttgcaggcac cggaagcaac gatacccatc actcaataga
     9781 actgtcaaaa tatgctgagt ctgtgggctg caacggagtt ctgtgcgtta ctccttatta
     9841 caacaaaacc acgcaaaaag gccttattgc acactacacg gcaatagcgg attccataaa
     9901 cattcccgta attatttaca atgtccccgg caggacagga attaatacaa acgcagatac
     9961 tttaaaggct ctgtccgtcc acgagaacat cggcggcata aaggaagcaa gcggaaatat
    10021 aagccaggtt gttgaaatag gaatgttctg cgatgataat ttctgcatgt attccggaaa
    10081 cgatgaccag gtggttcctc ttctctcggt cggcggaatc ggtgttattt cggtagtggc
    10141 caatatagca cctaagctaa tgcatgatat ggttgacagt ttcctgaagg gtgatgtgaa
    10201 aaccgccatg aagcttcagc ttgacataaa gaagctgaat gatgcccttt tctgtgaggt
    10261 caatccgatt ccgataaaga ccgctatgaa tcttctcggt atgaatgtcg gcaaactgag
    10321 gcttcccctt gtggatatgt cagaaggcaa tttaaacagg cttaagaacg aactgataaa
    10381 ctccggattt gaattaaggg ggtagcgtac ttgattaagc taatgataaa cggctgcaac
    10441 ggcaagatgg gccaggtaat tacaaggctt gcccagggat gtgatgacct tagtatagca
    10501 gcaggtgtga caagaaatcc atcaaggctg ccaaacagtt atcctgtata ccgcagcttc
    10561 aaggaagccg aagtcaaagc tgatgttgta gtggactttt caaatcctga aatgataccc
    10621 gagactgtag attactgtgt caagcatcat acagctcttg tagtaggaac aacagggctt
    10681 tcagagacag atctgaaaat gcttgagaag gcatcggaat ccatacctgt atttgtatca
    10741 gcaaacatga gcttcggcat cagggtattg attgatttgg ctttaaaagc agccgcaacc
    10801 ttaggggatg aattcgatat tgaaatactt gaaaaacacc ataacgaaaa aaaagatgct
    10861 cccagcggca ctgcaatgat gatagccaat gaattgaaaa gccggattaa tgacggcctc
    10921 gagtttgttt atgacaggtc gaaggtccgt gaaaagagaa agcccaatga gattgggata
    10981 tccgcaataa gaggcggcac cattcccggt gaacatacag tatttttcgc cggtaaggat
    11041 gaagtaatcg aaataaagca ttccgccctg tcccgtgata tgttcggacg cggtgcaatc
    11101 agggctgcac gttatatagc ttcaaaacaa aagggatatt acactatgaa aacaatgctt
    11161 gaggagctga tttgatgaat caaaaattgg atattactaa tccatatgaa atagccagat
    11221 atataaagga atccaaaaaa gtaacccctg tcaaggctta tgtcaacggt gacctgaagg
    11281 atgcggatat gagcggcata cgtgcatacg gtgcggacaa cttctggatt ttattcgggg
    11341 atcattcgga tatggagaaa tttctctccg ataataaaag cttaataagg agcttcgaat
    11401 tggaatatga caggagaaat tcagctattc ccctgcttga taccaggtat gttgatgcca
    11461 ggattgaacc gggcgcagtt ataagggata aggttcttat acacaagaac gctgtaatca
    11521 tgatgggcgc agttatcaat atcggtgccg aagtaggcga aaacactatg attgatatga
    11581 acgcagtgct tggtgccaga gctgttatag gcagcaactg ccatataggg gcgggttcag
    11641 taattgcggg agtccttgaa ccgccaagcg ccgatcctgt gataattgaa gataacgttc
    11701 ttataggcgc aaatgccgta gtgcttgaag gcgtgtgtat aggaaaggga gcagtagttg
    11761 cagcaggttc agtggtaaca aaaaatgtag agcctgatac ggttgtggca ggaatacctg
    11821 caagaatgat aaaaagcgtt aaggatttga acgaggataa gaaaaaatta ttggatgatc
    11881 taagaggtta ggatgtgaac acaaatggca ataattgtac aaaagtatgg aggcacctcc
    11941 gttgaaacaa ttgaaaaaat aaataatata gcagaacaca ttgtaaaaag aaaatcggaa
    12001 ggcaatgata tggtaattgt agtatctgcc atgggcaaaa ctacagacca tctgataaat
    12061 atggcacatg aaatcagccc cgatcctgat aaaagggaat tggacgtttt aatggcaacc
    12121 ggagaacagg tttcaatgtc tctattatct attgcgctca acaatttgga tgcagacgct
    12181 gtttctttta caggccagca ggttgggata agaacaaacg gctctcatac gaaatccaaa
    12241 atagctgata ttaatgaaga aaaaatccgt gaagagcata ggaaaggaaa aattgtcata
    12301 gttgcaggct ttcagggagt aaatgaatat aatgagatta ctacacttgg cagaggcggc
    12361 tcagacacga cagcagtggc acttgccgca aagctcgggg cccagtgtga aatatatact
    12421 gatgttgacg gtatatatac tattgatcca aggctttatc cgagagcaaa gaagcttgac
    12481 tgcatcagct atgaagaaat gcttgaaatg gcaagctccg gggcaagtgt catgcattca
    12541 agatctatag aacttgcaga aaaatataag gttcctgttc ttgtagccct aagcaggggt
    12601 gatataccag gtacaatcat aaaggagatg gacaatacca tggaaaatac ggcaataacc
    12661 ggtttggcgg ttaataatga tgaagcaata attaccttaa acggtgtacc ccatgatatc
    12721 aaggtaattg cggaaatatt ccaaagcatc gctaacaaag acataaatat tgatatgata
    12781 agccagacct tcccggtcaa caagcttgta agcatatcct tcactctgcc caagactgat
    12841 ctttatcagg cttcaaaggt actgaacaca ttcaagaaaa aattattcac cttcacttat
    12901 gaaacctgtg acaatataac aaaactttct gtcgtaggct tgggaatgaa cactcagtca
    12961 ggtgttgccg ccaaagtatt caacctcctt gcagagaacg acatacccgt cagcattgtg
    13021 acaacttctg aaatcaaaat atcctacgtt atcagccctg atgaccagaa gaaggcaatt
    13081 gaggctatcg caaaggaatt tgatttgtag ataaatttca agaataacgg gtaacactaa
    13141 aaacatgaaa atccacggaa tgaagagttc agtggatttc agtgaattca gtgttatccg
    13201 ttttttgcgg cttttatatc cccctgttcc cgaagccgta gcccagcact tctgctgtat
    13261 cgctcaccgc cataaaagca ctgctgtcta tctcgttgac tatctgcttc agcttagcca
    13321 actgcttcag cgccactatg cagtatataa ccctcttctt attacctgtg taggctcctt
    13381 ccccctccag ataggtaaca cctctgtcaa gagtttttag tattgcatct gcaacctgct
    13441 gttcattgtc ggtaataatc attactgatt tgctgcttcc gaagccctgc tgcactttat
    13501 caagaactgt ggatgccaca tacatagaaa ccaatgtgta catggcgggc tccagtccga
    13561 aaaagagtga agcaattcct attactatta tatttattaa aaatgaagtt tttccaagat
    13621 tcatggaaaa atattttttg actataactg caacaatatc aactccgcct tgtgaagccc
    13681 tgtttctgaa taccagcccc attcctattc cgttaagtac acctccgtat aaacatgaca
    13741 acagcgtatc ctcaatataa acaacattct tcaaaaatga gaacaaatca atggagactg
    13801 aaaaaaccgt catcccgaca aggcttaaca gaataaactc cttctccata aatttatagc
    13861 ctgcaagaaa tatcggaatg ttgaatataa atataagcac tccgggattc agcccgaaaa
    13921 tatagtttaa tatcagcgct accccgctga cgcctccgct taaaagctta tgaggaatca
    13981 aaaatgcatt aaaggctata cctcccagcg aagctcccag tactatcata atcagaatcg
    14041 gaacatatct gagttttctc atgattctat gccttccctt gccggaatcc cgctgttgaa
    14101 ataatgcttg atctccttca tttcggttac caggtccgcc gcatcaatta tctccttcgg
    14161 agctttcctt ccggtaataa taacctccat attgacaggc tttgacttaa gaagctcaag
    14221 tacatcattt acttccaaaa gcttgttttc caatacacct aacaattcat caattattag
    14281 gacatcgcag gaattttccc gtaacatatt ttttataaac tcaaaacctt tgtcaatatc
    14341 cttcttcagt tcgcgctttt cctgttcgga aagagtccag aaaaagcctc tttccctttc
    14401 aaaacggaat atttcaaaca aaggatacag cttctcgaca ctatggagtt cccccgtatc
    14461 tccgcctttt aaaaattgaa ccatgcatac tctgagcccc cggccgcagg atctcaatcc
    14521 ctggcccagt gccgcagtag tcttgccttt accgtttccc atatatacct gaacaagccc
    14581 cttttcaagc ttcatagctt cgacccctcc gtttcgtttt atgctcatcc atatagttta
    14641 ttataacttc tgtagaaaat atttcaaata taaataagta aaaaaaagcg cattgtcact
    14701 gcgcttttcc ttttattcct ccggttcttc ctccgaatta tctttcttct ctcctgtctc
    14761 cccttcaatt acttccattt ttgaaattga cacctcatat gccgttctgg ttattacctc
    14821 atcctcagat acttttttct gataatcgcg gctttgtatt ctgccccata tcctaatacg
    14881 atctcctacc ttcaaatttt ccgaatatct tgcatttctg ccccatgcaa tgcacggtat
    14941 ataatcagac ttgttatatg gcctgttgac ggccagcagc atatccgtta tctcgcgtcc
    15001 aaagggcgta gtcctgtaaa caggtttctt gcatatatag ccgtccagat aaatctggtt
    15061 cggattcttt acatcctggt ccatagtctt caaatttctt gcaaatacag taagtataag
    15121 tttattattc ccttcatgat atttattgta cgatctaagc tgtccttcaa caagaagagt
    15181 tgaccccaca tcaatattga gcccttgcac aagcctttcc gatatcgtta tcggaagcgt
    15241 atcggaaata tcactaagcc tcggaacctc cagatatacc gtataaaaac cctcgccgta
    15301 catttcgtgg ctgaattccg gtttggaagt tactgtaccg tatacggtaa cattgttgct
    15361 ctcgatgact ttatccgtca taagccccgc tcctttcctg ttcttgtgta tttcaggttt
    15421 aatctacagt atacatattc actacaaaca tataatatga attattttta aattcagaaa
    15481 agagtctgcc tcaaaataag cataaaaaat ggaatgttgt gtcctcatca gaaacagcat
    15541 tccattgctc tttttcttta ttattctaat tttttttctt catgggcccc agcagcttat
    15601 atacaatcct gcttgccgca tccatttctt cgcttcccag caacaccagc aaatcatttc
    15661 tgttaaccag cgacaggccc ttttcaatag catggtgcag caaatgataa tatctgaatg
    15721 aaatgtcatt ttccctgaaa attcttttat ataatttcat acttttcaga ggaagcgttc
    15781 cgatatggga atcaccatcc atacaactgg tcaggattac ctccttgcac ttcagaatcc
    15841 ttacccactc ggctatcagg ccggcttttt cctcatgaaa gttcatattc acatcttgtg
    15901 aaacagaaat gacaagtaca agattctcat aactcaatat ttgaattgaa tcaaaagctg
    15961 ccgtgtagtc ataagctgaa ttgcagtaat tatctataac agtaaagcca tcctcatata
    16021 ttttctgaaa atgcctggct gagcccttat agctttctat tgactcttta atatctgaaa
    16081 tgtccaagtc ataataaagg ccgcaggtaa ttgccgccaa tgcattatat atgctgtggc
    16141 ttcccaggac attcaaacgc accggtatct cgaagggttc aattcttgcg ccgcttccgg
    16201 acagaaaact tctctgcaca caataattga agcaggttat ctcatcaaca tcaatactgg
    16261 aagctgttgc aacagccttt ttattcagcc cgtaagtgat taatcctgcc ctcttgcagt
    16321 tttctgagac atttatttca taatactcat cattgttcat tattactgcc ctgacctccg
    16381 tcagccctga gacaaaatca gctgccggct tattcccgca gcatttaccc aaggcacacc
    16441 tgtcagtcag tatggcactg tcaaagctca tactgtcaag ataaaaaagg ttgttctcat
    16501 ttgtattaat agttataggt atgatgtcca tatcgaacct gctgttatct ttcccggaaa
    16561 aaatactctg cagcatccca accaatatac ttttatcact ttcgccaaat actccgatta
    16621 gctttgcatt acttttctgt ttctcgaaaa atttttcgaa cagcatgcaa agcgtatctc
    16681 tggggctctc aacctttatc accggcaatg caggatctga tatattgtaa ggagtgaata
    16741 tcatatatgc tcccttttcg taggcttcat atatcctcct tctgttcctt ctgcatgaca
    16801 aatcgacata tatcataccc ggccttacct tttgcggatc tgctgtaaca cctattcctt
    16861 catgactgtc cacgttcatt ttaccaactc ccggactata gttagatctg tacaatgttt
    16921 aaacgagggt aggaaaatgc agacacaagt gagggatatc ttttctcctc gtgattatat
    16981 aattggcata cagtcattta tttatgtatg gttccccggc tgtataaagc taattacctc
    17041 attttttatt tggtttaccc accggaagct cctttttgct gcctttctta tcaaaatatt
    17101 tcattatatc cgccaaatca ctctctgtag catcaagtac ctttgacaat gatgaagcac
    17161 catgaaaaaa cgcttccagc acaagcacct tcctgagctt ttttataaac cgtctatcca
    17221 tcatgactcc ctccctttat acattatatt aagggagtat gataattgac cgggctattc
    17281 atcaattttt tcaactttcc ttgggggctt cgggccgaaa aactgatata aatccgtctt
    17341 catcattccg ttataaagct tccttttttt atccgccctt cttccgaaaa acctttcaaa
    17401 gccctcatgg gaggtgataa tataaaagga ccaagtttca agccttgaaa atacctttcc
    17461 catttctttg tacagccctt ccacgctttt actgtcggaa agcctctcgc cgtatggagg
    17521 atttgtcact atgaagccgt acttttcctt gctgcttaac tcacttatat ctcttttctg
    17581 aaaatgaagc ttatcctcaa ggcctgccat cttcgcattt attcttgcta ccttaagagc
    17641 actctcatcg atgtcatatc cctgaatatt gaggacatca tcacgccttt caagggctcc
    17701 ggcttcctcc tttgccctat tccacagctt gcttcctatc catctccagt tgtctgaaac
    17761 gaagctcctt cctcgccccg gagcaatatt gagcccgatc atggctgctt cgataggaat
    17821 tgtcccggaa ccgcacaaag gatcaataaa ggccctgtct gctttccatg gtgtaagcag
    17881 caccatggca gcggcaaggg tctcctttat gggagctgca tttgacaatt ccctgtagcc
    17941 tcttttgtgg agggacaaac cgcttgtatc caagtacagt gatgccttat ccctgttaag
    18001 gaatacatgt atggggtagc gctctcctgt ttcttcaaac cactgctgct tgtattttgt
    18061 tttcaggctt tccaccacag ccttttttac tattgactga atatccgaag tgctgaaaag
    18121 ctttgacttt atcgatgaag ccttggctac gggaaactcc gcgtctttgg gtatccagcg
    18181 gttccactgc agcctctttg tcttttcaaa aagctcctca aaggaaaggg cttcaaactc
    18241 atcgatttta acaagtattc tttcagcggt gcgaagccac agatttgatc tgcatattcc
    18301 ttcttcatcc gccataaatg agactctgcc atcctctacc tttgttactt catatcccag
    18361 gctctttatt tcccttgaca gtatcgcttc taccgcaaaa ttgcagggtg ccaccaattc
    18421 aatttttgac acggccatcc tcctttgtga tatctgtacc tacttaatta agagtatatc
    18481 attaattata ctgtatccgt caattattat tcatctcagc agcttctgca tacctgtatg
    18541 atcgatataa taattctcct tgtatataag ctcggacacc ggcactatct tgtacccttc
    18601 cttctgcagg ttctcaagaa taatgggaag cgcgtcagcc gtataggcgg cattattgtg
    18661 aaacagcact atcgatccgt tcctgacctt tgagagcacc cgcttcacta ttgcgtcagc
    18721 cccgtaatcc ttgtaatcaa gagaatccac atcccactgt ataacttgta tccccatagc
    18781 tcttgaggtt tctataaccc tgttgttata atcaccgaaa ggcgggcgga ataaagtcac
    18841 ctttttatgt gtaattgctt caatcttttc cgaggtcttt gcaagctcgt ctattatctg
    18901 ctcctttgag agctgtgaca tatgcgggtg tcttgatgaa tggttgccga tttcatggcc
    18961 ttcctcatca attctcttta ccatctcagg atatttatcc acccagaatc caaccagaaa
    19021 aaacgtagtt tttacatcat aatttttaag tattttcaat aggctttctg tataatccgc
    19081 cccccatgcc gcatcgaagg atattgccac cttcttttcc ggagtatcca cacaatatat
    19141 gggaagctcc ctgttattgt ttctgaatac atttaaaatc tgtttgtacc ctgcataatt
    19201 gtatatcagc gaaacactga caacaataac caccagcatt atattcaaga tgtatttgtt
    19261 cttcgcgaat ctgaaattca taatgcacct cctgatatat tattagtatt aatctattca
    19321 aaatatcagg aagtagaata tttccaagca taaaacagca taatttggcg cgaaattaaa
    19381 atttcgcgcc aaatttcatt atattaacca gattttctac aatttttcaa tgctgttgtt
    19441 tagtctaaac atcaaagtgc aaaaaatgac ggacactcac ttcatcatac tgctccctcg
    19501 taaaagcagc accggcctcg ccttattagc agcaatggcg tgtagccgca caggctcctc
    19561 catgagtgtc cgtttttttc tatctcaatc atttatcaag taatgattgt atgtatcagc
    19621 tctataaatt ctctcggagt aaatttcgca tagtttacca gcaaaatagc tgctaatgca
    19681 atagcaacca acctgatcaa tgttgagatt gtaagggtat tcatcactct aaacacatac
    19741 tgcaacgttc ttttacaact gcgtttcaca gtgattcttt tccctccttt cacaccaggc
    19801 cgggaggtgc tgcaacaaga ctatgcttgt cttttatatt atatcaaccc ttcaattatt
    19861 gaagaagctg agctagtact attttaacca tgactatatt accatattta tacatatttg
    19921 gctgcggcat actattattt tactgcctgc ctattttctc tatgcatact gcaatcggag
    19981 gacaatttat ctggttcaca aaatcacata caagcacgga gtattttctg cagtcgattg
    20041 tccttatata ctccatcact gcctccttct cttcaaagcc gctgtcccct ccgtaatata
    20101 tgaccaccgt aattataccg ccgtctgtta gcatctccat ggactttttc agagcttcta
    20161 tggtagtgca ggcctttgtg ccgatactat ggtccccctt tggaagataa cccaaattga
    20221 acattaccgc ttttatgcct ttgggtacat aggcatcgat attctcatgg ccgtcacata
    20281 ttagctcaac gatgtctgaa actccagcct ccctgagttt ttttgccgtg tttatcagtg
    20341 ccgtgtcttg tatgtcaaag gaatatacct ttccttcgac ccccacaatg cctgcaagaa
    20401 gcagtgtatc atttccgttt cccatggtgg catctacaac tatatcaccc tgcttaaccg
    20461 ctttttttat tatctcatgg gaaatagcca gtgaattgct gagcagcata tgaaaagctt
    20521 cctttcttta atagccgtac cttcctgagt attggaaggc atccttgata aggtttatcc
    20581 tgtaattatc ctcaccatca ataataattt ccattacatc aaatctgcac atgcggtttt
    20641 gatatccatt ccgctgcata taataaaggg ccgccgtgac cagcttattc tgcttatttc
    20701 tgcttattgc ctccgcgggc agtccgtacc tttcactgct tctggttttt acttcgataa
    20761 aggcaattat ttcaccttcc aaagcaatta tatcaatctc ccctgtcctg caggcgaaat
    20821 tcctttccag cactctgtaa ccgcattctt caaggtattc acatgccttg ttctcgccga
    20881 aggctcccaa tattttattg ctgcccatac ttttcccctt tcagcttgtc tatcttcgaa
    20941 acaaatacca atacctcagc cacaacgctg tataattcct gcgggatctg cccccctata
    21001 tccagctgca gcagtgcatc gaccaggccg ctgtcttcaa atatgggcac attgtttttc
    21061 tttgcggttt caataatacg gttggctatc tcacctttgc ccaatgccgt aacccttggg
    21121 gcatcatccc ctgcctcata ggtgagagcc gccgcttttt tcagcttctt atccatacta
    21181 taccctcata tcaaaatgaa ttttttcata tccgctttca tttatcattg cttcaagaga
    21241 aaggaggctg aagggctgct ccagatttat taccctaatt gggctgagat aatagccttt
    21301 ttcccccagg gcttctttca gcattagtat attcttttct atcatgcttc ctatgctctt
    21361 attttctgtc ctgaaggtta tatcaaccgt tttgcccttt acccttatca tgctctccaa
    21421 atgccccagg ttcatcaggt ccagggctac aacaacagtt gcgtcttcag ggtttatcct
    21481 cttttccctt ttgccgtctt taaatacata cagctttgct gcggttgtat tgtcatttat
    21541 cagtaaagga atctgaagaa aattattatg ctgattaaca ttgcgaataa aatcaataca
    21601 atccttgagg tttgacaatg cctcccttat ttcagggtct ttatccccgc cggttttcag
    21661 atagctttcc agcttttctc ccaggcttgc aatatcctta agctgccggg caacttcttc
    21721 tctgtcctct aattgcctgg gctccagaaa taccttcctt atggattcac ttatggcttt
    21781 aagctttgga ttatcgtctc gaataatctt ttcctgcagc ttgtcaagga actgagatat
    21841 cacatccctg ttctcgatgt tttttataag catgcccaga ttcctgggcg ttatctccat
    21901 ttccttcgaa accagataag taatggcttc catatcggta ttttttattg aagatagtat
    21961 gctttcgact tttgccgcaa gcatttttat ttcgctttca gattcaattc ccagtccggc
    22021 caatgctctt atcagttcaa ctcctttatt gctttccgct gcagccatat cgctcaaagg
    22081 gctcacataa ccgacaccct ccttgggagt ctcacctctg cctgttattg cagcctctct
    22141 gggagtctca ttgctgtctg ttattacagc ccccttgaga gcttcactgc tgtctgttat
    22201 tgttataact gcagcctttc cgggaatttc tgctgccttg ctgtcactag ccgatcctgc
    22261 agttgtttgc ttaataatag ctgcctcatt tgcctccggt ttctgaccgt cattttccag
    22321 tatggcttca aaagccgcaa aatcatccgg ccagttgagt actatcttat tcaatacatc
    22381 aacgggcgta tcctttatat tcaatcccga tagcatcagc cccattttct ctccggtgct
    22441 gttattaaga ttatcaatgc ttttttgcag ccctgtgata ttaccgattg tttccctgtt
    22501 cagaggaagc ttatacttta gcaggagctt tgcagcctcc atgtttttct cattaaccgg
    22561 cagattgagc tgctttaaga gatcggaaac ctttatatcc ggatcggagg ccttatcctc
    22621 ccctttcagc tccgcaaata tcttcccgtc agctatatcc tttataacca attcgacaaa
    22681 agcatccttg ggtatacttg ccccttcctg aagaatggca gtaaatatct ctccgctttt
    22741 cgcccttatc agtatgctgt ttcccagagc ttccagcacc ttgcccttca gtacatctcc
    22801 tgtctccagg tttttaagga gttcaataag ctccggtttt attaatctaa agcttgaagc
    22861 atctatcttc attataatca cccctgaaaa ttcttggtaa aggatctcct gtggatgggc
    22921 agaagcccgt atttcttaat gcactcaata tgctcccttg ttccgtaacc cttgtgttta
    22981 ctgaatgcgt attgagggta caactcatca taggcggata tcatcctgtc cctttccacc
    23041 ttggctataa ttgatgcggc agcaatggaa aggctcaggg tatctccctt tatgattccc
    23101 ttctggggta tgtccatatc cttcaaggta acagcatcca gaagtatgta atcaggcctt
    23161 tctgccaacc catccaaggc tttcttcata gcaaggcagg cagcattcag aatgtttatt
    23221 tcatctatgg ttttttcatc aactattcca atgctgcagc acagggcttt ggactttatt
    23281 tcatcataaa gatattccct tttctcctcc gacagcttct tggagtcatt gaccccgggt
    23341 atgagcactc cctctccaag aatgactgca gctgcaacta caggccccgc cagtgggcct
    23401 cttcccacct catctacccc tgcaaccagc ttatagccct tctcatagca cttccgctca
    23461 tattccgtca tccggataag ccgttccttt tctttatcgt catactttat ccttacagcc
    23521 atttttacac ctcattcgta tatatagaat tatcggatat atctgcataa aaatataagc
    23581 atatacattt tgatgtacat gcttattata gcatattgtt gtggcgatgc aggagtttga
    23641 gttattttat tctgaaattg aactttcatt gatattaagt attttaggaa gcaccattca
    23701 attcgccaaa tgatgatcca ttgccaaaaa cagtctgcaa aaacattgta acagccaact
    23761 aaaaaattta tggtatcgaa agaaaaaaca aaaatttgag atttttgata gccccgaatt
    23821 tgcttttgtg gcctgtatgg aagatatcct ggatttgtat cagcagccgc cgataaccgc
    23881 gtttctaatc tgtgtgttat cggctctgcg cctgtgcgtc tgcctctttg ataactgaca
    23941 tattcggtta tttgatattg actagcgttt tctgtttacg cttcggatag aatagtggga
    24001 agtttttaag caccgtagcg gggagtattc taatccgcgt tttgctttga caaacaggac
    24061 acaatatcca ctcaaaactt ctgatctcac caacacttgc ctaagactta tcacttttaa
    24121 tcttcatgtt tctctctaat ttttctgttg attttatcag cttgcagctt aaaagcaagc
    24181 atgaaaattg cccaaatgat tgccgctgct cctaatgcta tcaaaatgta aactactatc
    24241 gctccaaaac tcttttcaat ccacccagca aagaaagcgg taataagata tacaaccgtt
    24301 cctgctgcca tatgaataag cacttttaag ctgttcgtaa ggttttcctt ttcgtaaacc
    24361 aaagagggga cggaaaatcc aatcgctata ataataaaac atgcagcaag ccgcaacatt
    24421 tcttcgcctg tcttttgacc gatgaaagcg tttgcgccgc ccgcgaaagc ggaagcgata
    24481 aacagcatgg acacaaaccc aaagcaccct atcgcaactc caaattcaat ttttacaagc
    24541 agttttttca taaaacacac ctccctatat gccaagatat tttttgaaag caggcagata
    24601 cattcgtgag atatagtcct tgcacccgtt tttcagaaca agcagcataa ttcctccaaa
    24661 ggacggctca acgcaatcaa gatatttcag attgaccaat acggatttag atattctgat
    24721 aaagccgctg ccgagtattg tttcaaattc atataagcgt ttattgctgt tgtatttttt
    24781 gactttagta tatacaacgg tcttttcgtt ctctacccgt aacatataaa cttcttccgg
    24841 gcgtaagaca aacatgcgct cgttgtcggt aacggtaatt atgctgttgt ctgcgctatc
    24901 ttcaatgaat gaggctatgc ggctgatttc cgctgttatt tcattggtat atataacagc
    24961 gtatggctct atgacttctg cgtatatttt aatttctact ttcactcgta tctcacctcg
    25021 ctttctgctt gcatgtacat tatagggtag ttgcgcgtgt ttgtccgggg atttcaggta
    25081 agtggctgat tttaagaggt aacatgcaaa tactcagttg tcacccattg attaagttgc
    25141 ttttttctgc gtctttatgg taacagccac attctataaa actttgcata ccagggattt
    25201 tctctcaaag tttccgtatc cgccttaaga tatgccccat ttcatcaccg cctatttgtt
    25261 gacaattacc ttgactaatc ttcaagaatg aaaatgtcct cgacagatga ttcaagaaat
    25321 cgggcaagtt tcatagcaag ctccaaagta ggattatact tgtcgttctc aatagcaata
    25381 attgtttgtc gggtaactcc caatatttgc gctaaatctt cttgccgcaa gcctttatcc
    25441 ttccgcaaat ttcgtatatt attcttcatc atcgctttcc tttgtcattt tactcgtaat
    25501 gaatactttt gaaatgaaga acagtacttc ttgcacacat acaatgacaa aaggaattgt
    25561 aggcagtctt ttcaacgcca catattcata aatacaatat gccaataatg ctatgattga
    25621 aaatacaaaa gcaatacgca tagctctgta atttatggcg atttccattt catcagcttt
    25681 ccatttctga tttttcatat tgataatcct cctatatgta aagaactctt tacatttcct
    25741 attatactca cagagcagaa gatgtcaaga tgtttttaca ttttactttt taagtttttt
    25801 gttacggaaa ttgaaaatac acgcattatg aattatttaa gctaatagcg ggtactgtgt
    25861 aggtatatcc aaatagaaag gtaaactgca atgctaggac ggtgaaaata ggggttaaag
    25921 atatgcgtat tgttttgtta taccagtcaa gcgttccaat atatgagcaa ataaaagaac
    25981 agattaagac ctcagtttta tctggcgagt tgcaagaaga tgaattgctg ccctctattc
    26041 gtcagcttgc tcgtgactta aaagttagcg tcataacaac tacacgagcg tataacgatt
    26101 tggaacttga aggtttcctt caaaccgtac cgggtaaggg gtgctatgtt aaagaattga
    26161 ggaggatttt ataagaagcc aatatgttaa ggagacaaaa gccacgttga acaccgctat
    26221 tcgacaaagt aaaatggcag ggttgacaat gtcggagctt cataaattat tagatgaact
    26281 ggaggagacg tataatgaaa aacgcgatta tggtaaataa cctcacaaaa aagtataaag
    26341 gctttctgtt gaacaacatt agctttactg taccaagtgg ctttatttgc gggtttatcg
    26401 gctaaaatgg tgcgggtaaa acgacaacga tgaagcttat gcttggcatg gctttaagag
    26461 atggcggcga tattgaaaag gctctgtatc cattctatag gcagtgggac agttcggcgt
    26521 ttcatcagta tcttaataaa ttttctctta atcccaaaca aaaatacaaa accatgtcgc
    26581 gcggtatgaa aatgaaactc ggtctagcgg ttactttatc ccataacgct aaactcctgg
    26641 tgcttgacga gcccacttcc gggcttgacc ctgttatgcg tgatgaagtg ctttatatac
    26701 ttcgtgaata catggttgaa gaaaataaaa gcgtattctt ttctacccac atcacaagcg
    26761 accttgaaaa aatcgcagat tacattatct atatagacaa aggccgaatc gtatatagtg
    26821 ggctcaaaca tgaactgatc gataaatact ccctaatcag gggcggtaac ggggacttgc
    26881 cacagtcaaa acgtaaaagt attataggac taagagagca tatcggcgtt tttgagggta
    26941 tgattgaggt tgcggaaatc ggaggtttcc cgccgggcgt aattactgaa acagtttcac
    27001 tagatgatat tttggttcat atgaacagga agggatcgga gttatgattg ctaaaatgat
    27061 tgcccttgat tggcgtgcaa tgaaatatta ccaaataaga ttactgctgt tgccggtttt
    27121 cgttttcatt ttcggggttt actctccgtt gacagtaatt cccatgagcg taatcctgtc
    27181 attaagtttc tcattaaatc ctttcgcagt tgaggaaaag ggagagttaa ataatcttta
    27241 tttaacctta cctgtgagaa gaaaatcaat cgtcgcggga agatatattc tatctataat
    27301 aatgctgtta tgcggtatag caataggtat tctcattatg ccgcttgcga ataaactttc
    27361 tatatcaaaa tggtttattg gaatagaaga gtttatagtg gtcatatcat tgagttatct
    27421 tttgtatgcc attcttaatc tttttatgtt tcctatgcta ttcaggcttg ggtatcataa
    27481 aggtaaattt tggggctttt atttacctat ggtttttttc gctttgcttt ttggagcata
    27541 cttggcaatt acacacttgc cggggaataa aacgcttaca cttaatttca ttgtatacgc
    27601 ttcagaaaat ttgttaatgg taagcggaag catagttgtt ttggcaacta tgcttttaat
    27661 cctctctttt atgatttctc ttaaactgta ttccaaacgc gacttttaaa caaatgagcc
    27721 tgagccttgc ggagcaggta tttaaactta tcaccgcttt gacttcctat aattcgtcct
    27781 gttctttttc gtctaactgt ttctgaattc ttgcctgttt tttcttgttc agataatgaa
    27841 agcaatagag tactacaaaa gaaaaaacag tcgcgctgat aaaagtaatt gccaacacgg
    27901 cgataaaata acctatacca tcttccttat agtgttctgc atactgtgta tagttcgaaa
    27961 caccggttat cgttgttact actattccgg ttactatacc attaataata gggatggttt
    28021 tagcacgttt tccttcgccg tatatattga gtcccaaggt caggtgtcga atcatcaagt
    28081 ataaggacat cccgaagaag catataaact caacggcata ctgttcaaac ggagcgttca
    28141 acagaaactg ctgtacgagc attgaacaga gaagcgcaat cattaaaatg ccatatgctt
    28201 cgctatttat tttacggcgt tgtgctacaa ctctttcgtc ttgcgaattt tgctttttca
    28261 tttcgattcc tcccaaaata aatcatttaa tgttttgcta agttccttac aaatcgctat
    28321 acaaaggttt agcgtggggt tatattcccc tgattctatc agactgatag tttgtcgggt
    28381 aacgcctacg gcttttgcca agtcctcctg tttcatatca acttccattc gggcaatttt
    28441 cattctcttg tttttcataa aatgcctcct tttatagtca tagtatacac tatttattgc
    28501 atggtgtcta ttatatattg catttcggtt tgcaattaag tattaagttt atatttgtta
    28561 aattttcatg gcaactgctt tctatgtaaa aaagaaaaaa cccacaggag caattctaaa
    28621 gactcttgtg gggtttatgt cactctaaaa aaatataaat accctgttac gctgaaatat
    28681 gcaaccttgc tatagccatg tatttattcc tgtccattat ttaaataacc caaagatcaa
    28741 atcctgcgca acaaatccat gtttattaac gataagctgc attttttaca tgcggttatc
    28801 aatcctgacc acacaacctc aaaaatatcc gaaaaaacat ctaacctgac cactcgtcaa
    28861 cgctgacatc taacctgtac actcactgag ctatgacatc taacccgacc acttaactat
    28921 caaaatatct ctgttggact tgttcccacg aagcgatatt ttgacgattt tcattcaata
    28981 ggttttacac ccatatgtgc caaaagcctt ggtatataag gctttttcac aacttcactc
    29041 atttaccctt gttatcaata ctatagtctc cacgtggctc gaccgtatgt ttttaattac
    29101 aacaaccgca cattttcaac ctattggttc ggtagataca gtccacactt tttgtctgtt
    29161 cgatagatgc agtcaaccaa atttgaattt gactgacggt gaatatagaa gaatacgatt
    29221 taaagcaagc aaggcactct acgaaattcc aacgtagggt gccttattgc attttacctt
    29281 caggttaact actttttaag tttctctatt tcttcttcta cttcttcggc cccttcagcg
    29341 taaaattttt cctcgtcagg gtcaagttct ttaagtaatc tcaaaaactc accggcatga
    29401 actctttctt catcagcgat atcctttaaa acctcgattg ccaatttgtt atcggtcgat
    29461 tcagctaatt gcatatagag ctgaatcgct tcatattctg cagcaatcat aaaccgaatt
    29521 gatctaatta attcctggtg actaatctta tgttctttct ttagtccgga aaagggttgt
    29581 ccaaaatcag gcatattctt tacctccaca ataaaattta atagttttct tttcttacct
    29641 caaaatagct ttgagcaaat ttacatacag gacatacttc aggagctttt ttaccaatta
    29701 ccagatgccc gcatacacgg cattcccaca tgatttcctc acctttttca aatacctgct
    29761 gcatctctac attcttcaat aatgcacgat aacgttcttc atgtgctttt tcaatagcgg
    29821 caacattgcg gaatctctct gctaaatctt taaaaccttc ttcttcagca tccttcgcaa
    29881 aacgatcata catatctgtc cattcataat tttcaccttc ggctgcatgt aaaagattct
    29941 ccgctgtttt tcccagatta ccaagctctt cataccagat tcgagcatgt tcctgctcgt
    30001 ttcttgctgt tttcaaaaag atttctgata gctgatcata accttctctc tgagcaatat
    30061 ttgcaaaata agtgtatttg tttcgagctt ggctttcccc tgcaaaagcc tccataagat
    30121 ttttctctgt ctttgttcca acatattgat ttccagagtt agttttctct tcaactttta
    30181 caaatactga agctggttgt ttacatcttg gacatgtaaa tccttctggt aattctcctt
    30241 catgaatgta tccacaaaca gaacaacgat atttaaccat ttttttatcc tcctgtaatt
    30301 tgatattatt agttttaaaa ctatccaaca atttttttac aacctcaatc ggaaaatctt
    30361 ctgattgttt tttatcaagc agtgaattta agaaagccct agcattttca ctttctggaa
    30421 gcatatatcc tgcttcacgt ataatatctt cagccataat atgattggat ttttctatag
    30481 ctatagccag tttgccagat agtccggcag caatgttttc ctgttctgac ttacttcgta
    30541 ggagcacatt aagtgaattc acaactgttt catttttaga ttgttgagtt ggatattcta
    30601 caggcaccat cgaaatcgcc ccggatggac aggcatctgc gcaatctcca caaccaatgc
    30661 atttggcaac atcaataatg ctgttctccg tatctgttgc tccggtaggg cagacataga
    30721 ggcacaagca atctttggta cataaacgta catttcttac cgcatatttt ttaggcattc
    30781 ttatgacctc ccttcaatct tttcaaattt ccaatttggc accttgcaaa caggacaaac
    30841 atctggaaga ttatcaccta tgtaaataaa gccacagata gtacaaacat atacaccagt
    30901 attttcgagc attgcatcgc cctctttctt ataacgagtc aaaagcgact ttaaaatacg
    30961 agtaaccttc tcactccaga ctaaagcgcg aagcgcccct ctatccttga catcagcagc
    31021 caaagcgttt gcgttcggga aaccttcctc aaggtctttt tcaattaatg caatcatctg
    31081 atcaatatcc ggagattcag caggcgctga tgccttctta aaatatcccg ccaattcatt
    31141 gaagagggct gcttcttcaa gcttatactg tttttcgcag cctcgcgcaa gattagtgca
    31201 aagagcacta atctctaatg cagacatttc ctttatatct gttgacggct caattacaaa
    31261 tgttggtttc ttcacttcag tagaagcaat ctcaccctgt ttctcaaact ctggttttgt
    31321 tgcaccgcag agtggacaaa tccaatcctc cggcaaatcc acccatgctg tacccggtgt
    31381 aatacctgct tcaggaattc cctttgcttc gtcataggca tatccgcaga cagaacatac
    31441 atatttagcc atatcattac ctccttcatt tgatttcatt tatattagaa aggcatcttg
    31501 gacaaagtcc tttaaaatat acatttttat cattgatctt aaatttgctc aaatctccag
    31561 tgaccaaaga atctatatca atgggaaaat caaatatagt cccacaggat tcacatttaa
    31621 aatgtccatg attttcagtt acaatatcgt atctagtttc attatcttcg attgtaatta
    31681 cccttaccaa acctgatttc accaaaattc tcaaagtatt atatacagtt gtttttgata
    31741 gcgtaggtat atccttttga aggtcggtaa atatctgatc cacagttgga tggcaatgat
    31801 tttggacaag atattctaaa accttcaacc gctgatgcga aagataaata ttcgtcatct
    31861 ttaatttttc ttttaattct tcgaatgagg atttcatata atcaccgtcc ctgatataat
    31921 ggaattttag atttgttatt attataaggc tgtaaccgtt acaaattcat tatacatctc
    31981 ttatacactt atatcaacaa gattttaaat aaatatcttg ctttccccaa caggaacaaa
    32041 atctcctgat cgaaggcctg tctccaagtc tcgtttatac ttctacttca attcctgact
    32101 taaacctcac aaccacgttg ttttcaaata tagttgcttt ctcaactaag aaccttatca
    32161 actcttcatc ataatcaagg ctctggtcag cgtggttttc tattaagctg tcggtgcctg
    32221 tcaccacgta ttaacttatt ttggtgttga aattccaatg attatatggc ataaatttta
    32281 tcaaaatatt actttgaatt aataaattaa cttattgata aagagatatc tttgttatta
    32341 tgccgctctg aattaaggga tattatgtat tttatgtggg gttttaagca aatgtaaacg
    32401 tcaatataaa agataccaaa aacgcgcata ttttggatac cactttg
//
LOCUS       DAOQUR010000025        31754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1650_length_31754_cov_11.205022, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000025 DAOQUR010000000
VERSION     DAOQUR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 31754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31754
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1650_length_31754_cov_11.205022"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..731
                     /gene="moaC"
                     /locus_tag="PKI50_06930"
     CDS             186..731
                     /gene="moaC"
                     /locus_tag="PKI50_06930"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNT03473.1"
                     /translation="MNSKNKKEVQGVGELTHINAKGYAKMVDVSEKEESVREAIARGS
                     VYMKRETLKAIAEGRIRKGDVLSVAQVGGIMGAKSTPDIIPMCHNVSIYGADIEFQAD
                     YENSKIDIEAVVKTVGVTGVEMEALTAVSVAALTIYDMCKAIDKLMVISDIHLVHKRG
                     GKSGEFNFSGKLNEDINNTKC"
     gene            750..1247
                     /gene="mog"
                     /locus_tag="PKI50_06935"
     CDS             750..1247
                     /gene="mog"
                     /locus_tag="PKI50_06935"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNT03474.1"
                     /translation="MEGFSVGIITVSDKGSKGEREDASGPAIEEIMKQIGGRVEKYVI
                     VPDEMRDIKEAIIDMSDTLGLNLILTTGGTGFSKRDITPEATLEVIERYVPGIPEAMR
                     AKSLQATPKAMLSRAQAGIRKQSLIINLPGSPKGVRENLEAIIPALKHGIEILTGQAS
                     ECGGK"
     gene            complement(1253..1741)
                     /gene="mobB"
                     /locus_tag="PKI50_06940"
     CDS             complement(1253..1741)
                     /gene="mobB"
                     /locus_tag="PKI50_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNT03475.1"
                     /translation="MIPVFSVVANNSKTGKTTVLCDIVRELKSRGYRICTIKHDVHGF
                     DIDHPGKDTWKHAQAGSDFVVISSPKKLAMIEEVEKEYSLDEVISKISNVDIIITEGF
                     KQERKPKLEVYRKEFPNEIWSKDEDLFAIITNTPLNKGVPQFDFSEIKEAVDLIESLF
                     LK"
     gene            complement(1738..2973)
                     /locus_tag="PKI50_06945"
     CDS             complement(1738..2973)
                     /locus_tag="PKI50_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNT03476.1"
                     /translation="MLENITVETAKRILSEEKIDIKQSELYILDSLDHVLAEDIVSET
                     DMPPFDRSPLDGYAYKSEDTVNAAENSPVTLEVIDYVQAGYVSRKKICNGQAIRIMTG
                     AKIPEGADVVIRYEDTEFTDKTVKIFRYLKPQSNIVKAGEDMRAGDLILRKGITIGPA
                     EIGVLASNGKTHVKVYSKPTIAILSTGDELLEIDEELADGKIRNSNSYVIAAQVKKYG
                     GNAKILGICMDRVNDVKKKLDSALKWADMIITTGGASVGDADIIKEAFKAAGADILFW
                     KTRMKPGTPIVAAKHGNKLLFGLSGNPAAAFITFEIFVGPAILRLMGKPKHKYMKVES
                     ILESDFNKVSEQDRYLRAFTYKKEGAYYTVLPDKHSSGVLSSLSGKNSLLLIPGGSSP
                     YKKGDRVTVELINHLEAEL"
     gene            complement(2983..4005)
                     /locus_tag="PKI50_06950"
     CDS             complement(2983..4005)
                     /locus_tag="PKI50_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNT03477.1"
                     /translation="MNTVKTENAVGMVLGHDITEIIPGEYKGAAFKKGHIITENDIER
                     LLRIGKEHVYVFELGEDELHENDAALRLGKLFAGPGISFSEPSEGKTSLKSMYRGLLK
                     INRDLLEQVNDTEDICLATIQENICVEKDALLGGCRIIPLSIKKEKIEHVENMVKGKD
                     PMFRIKSFINLRTALIVTGSEVYKGRIKDEFGPVVEKKVKHYGSGIFNKTIVTDELDE
                     IKNAVLDAKKAGAELIVVTGGMSVDPDDKTPGAIKATGADIITYGTSVLPGAMLLFAY
                     LDGTPVFGLPGCVMFAHTTAFDLLLPRVFAGEKIIRKDITRMGYGGQCLSCEVCKFPY
                     CNFGKH"
     gene            complement(4073..4408)
                     /locus_tag="PKI50_06955"
     CDS             complement(4073..4408)
                     /locus_tag="PKI50_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT03478.1"
                     /translation="MYNIKYKLWLDKNGVIFGTGSYLLLKGVKEKGSLAGAAKAMNMS
                     YNKAHNLIKKIEIRLGFRLLYSKTGGAGGGISLLTIEAEELVSAYELFYNECEEAINT
                     IFKKYFPDH"
     gene            4795..7491
                     /gene="fdhF"
                     /locus_tag="PKI50_06960"
     CDS             4795..7491
                     /gene="fdhF"
                     /locus_tag="PKI50_06960"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5842..5844,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNT03479.1"
                     /translation="MVTFTLNGKVVTAKEGTTILDAAKENGVDIPHLCYDKNLSAFGG
                     CRLCVVEVQGAKALVASCTNTVSEGMVIQTESETVVNVRKDLLDLLLASHPEDCLTCE
                     QAGNCKLQDYAYRYGIKRSSYKGEKKSYDIESLNPVMERDQSKCILCGKCVRVCREVQ
                     VTGTIDFINRGFETKIATAYDVPISTENCRLCGQCISVCPTGAIINKQLKGTRPWEVS
                     KVRTTCPFCGVGCNFDLNIKDGKVVGVTPNPDSPVNGTSICVKGRYHIDMINSPERLT
                     KPLAKKNGEFVEISWDEALHTVTEKLKETKEKYGPDAIAGLSSARCTNEENFLFQKMM
                     RVAVGTNSVDHCARTUHAPTVAGLAATLGSGAMTNAISEVEGNEVLFIIGSNATEAHP
                     IIGNKMKRAARNGSKLIVIDPRRTELAEYATLWLPLNSGTDVALINGLMHIIVKEGWH
                     DKEFIETRCEGFDELLKAIEKYTPEYVSKITGISEELLYETAKLYTSTKKAGIFYTLG
                     ITEHITGTDNVMSLANLAMLVGHLGIENAGINPIRGQNNVQGACDMGALPNVFSGYQS
                     VTDEKAIQFFEDFWKFKLNRNTGLKIPEMFDGSHEGKVKAMYIMGEDPVLTDPDANHV
                     RKALEKLDFLVVQDIFLSETAKLADIVLPAACYAEKEGTFTNTERRVQMVRKAVEAPG
                     EARPDWEIICDVAKGLGAGGFDYKDAEEIFKEIAATTPSYRGMTFARLDKNGLQWPCP
                     TEDHPGTQYLHKGTFPKGKALMKAIEYKVPAEAVDEEYPILLSTGRMLYHYNITSRFS
                     EKLDAIKPYELAEINPADAEKLGLKEEDFVRVTSRRGSIVTRVTITNKVKPGMMSMTF
                     HYRESPANELTNSAFDPVTKTGEYKVSAVKVEKVKDMQGTAV"
     gene            7618..8208
                     /locus_tag="PKI50_06965"
     CDS             7618..8208
                     /locus_tag="PKI50_06965"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025434778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNT03480.1"
                     /translation="MSLVNTAVILAGGKSTRMGFDKQLIRVKDICIVEHLVEMLSPVF
                     KNIIVVTNKPELYKDKNIAIAEDVYKGYGPIGGIHAGLSKSQSMYNYFIACDMPNINK
                     CYIEYMIKRLEEDSFKSDAVITKKADWIEPFNAFYSKKLIPAIEDNIKKGKVRTMELL
                     EISNVLYIDEAIARGFSPDWSMFANLNTREDLKGLV"
     regulatory      8319..8438
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            8575..10314
                     /locus_tag="PKI50_06970"
     CDS             8575..10314
                     /locus_tag="PKI50_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNT03481.1"
                     /translation="METVNITIDGIKTQVPKEFTVLEAAKSLDINIPTLCHLKGINEV
                     GACRICVVEVKGARTLQASCVLPVSEGMEVKTCSPAVMEARKTILDLILSRHERECTT
                     CSRNLNCELQKLCDEMGIDKVSYEGEKIVYAVDQASPSIVRDQNKCVLCGRCIAICRD
                     IQHTEAINFANRGIRTTVTAPNGKALTEVNCINCGQCIKICPVGALREKDDTHKVWSA
                     LGNPDLHVVVQTAPAVRVGLGEEFGMPVGTNVEGKMITALKRLGFDKVFDTDFSADLT
                     ILEEGTELLNRIKNGGKLPLITSCSPGWIKFCEHNYHEFLDNLSTCKSPQQMMGAVAK
                     SYYAEKAGIDPKNIFVVSVMPCTAKKFEANREEMALDGLKDVDVVLTTRELAKMIKQV
                     RINFTALSEGKYDSILGDSTGAAVIFGATGGVMEAALRTVADILTGQDLKKIDYTPVR
                     GMEGIKEASVNVDGVDIKVAVAHGTGNAAKLLDSIKDGKAEYHFIEVMACPGGCINGG
                     GQPILLDKEKTEEVKKLRAQGLYKIDKEDRRFRKSHENPEVKKLYEDYLEEPNSHISH
                     RLLHTKYIERDRA"
     gene            10320..10793
                     /locus_tag="PKI50_06975"
     CDS             10320..10793
                     /locus_tag="PKI50_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNT03482.1"
                     /translation="MKGKLNLLTKAAVLLAIAIVFQLVKMGQYVTGTGINAVLITAVG
                     VCGLPWAAAIGIMTPMLAVLLGVQPPPTIVLVPFIMAGNTVYVVLFRLLRRYNDYIGI
                     IAAALTKYILLYTAANIIVDKLPAPIRLAFSFPQLITAAAGGAIALIVLKIVQKK"
     gene            10940..12058
                     /locus_tag="PKI50_06980"
     CDS             10940..12058
                     /locus_tag="PKI50_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HNT03483.1"
                     /translation="MRDIMADKLTKAVIGLMLLVMLLFVCLEKPAGARKVFSGLSETE
                     DMTDSIHQIFRNRNNAIINGDLKLIQSLYDTSTKYGTWAHQHELTKVKYLHKWEKKQG
                     VKFTEIIPTVKVRSIKPKGEKYTINLMCSTEYKYVYENEPGVVNSFRIGTYHVLDLLY
                     KDEVWIITREWYKDPFEDSLNLGRIDVDSIHQYISTHAARDFSNIGDRRKNAVAYADK
                     YCGAASEEKYGYKYNKKYRNYNSQGGDCANFASQILHEGGKFRKTHSWNYNNKGATRA
                     WVNADGFKSYMLYSGRASLIAYGNYEKVYKASYKLLPGDFIAYEKKGDITHISVVTGA
                     DSKGYALVTCHNTDRNRVPWDLGWSNGKIKFWLIRVHF"
     gene            complement(12162..12638)
                     /locus_tag="PKI50_06985"
     CDS             complement(12162..12638)
                     /locus_tag="PKI50_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNT03484.1"
                     /translation="MNKRMNILMFSGEYDKAMAALILANTAREIQVEVTMFFAFWGLF
                     LVRDPEKFSAKDKTAYEKIFGAVTPKGPEQMPLSRMNMAGLGKKMLLEMMDDDSAPHL
                     IDFLKGARKKDVKFYGCKLSMEIMGLKQEEMISELEIMEAKDYLKDALESDLQLFI"
     gene            complement(12802..14133)
                     /locus_tag="PKI50_06990"
     CDS             complement(12802..14133)
                     /locus_tag="PKI50_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT03485.1"
                     /translation="MLVKNNANYRDLIVGADTGIPLSNGQIAASINFDNAATTPPFAS
                     VIQAIEDFAPWYSSIHRGTGFKSKYSSEIYENSRETIKKFVKADPGSLVVFVKNTSEA
                     INKLSYRLYHKNRRSVILSTCMEHHSNDLPWRNKYLVDYINVDNNGRLSMRDLERKLH
                     KYRKNLALVTVTGASNVTGYKNPIHDIAELVHKYDSKLMVDAAQLIGHSCVDMKPDGS
                     SRHIDYLAFSAHKMYAPFGTGVLIGHQSDFEKGEPEYRGGGTINIVTHDLIKWAEPPH
                     KEEAGTPNIMGVVALAEAVRTLASVGMENLEAYESSLLEYALDRISIIPDIKLYCDDD
                     PRDRVGIIPFNIAGLHHQIVADALSAESGIAVRNGCFCSQPYVQRLMKIPRNDIRKRI
                     KNPSLPHPGMVRLSFGLYNNYDEIDVLVNKLYEISANKELYKERYGQHLTH"
     gene            14304..15104
                     /locus_tag="PKI50_06995"
     CDS             14304..15104
                     /locus_tag="PKI50_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNT03486.1"
                     /translation="MRRSKLLIFFLVITLSLTSVTFADTLKYGSRGAEVTKLQEQLKK
                     LGYFNGKATGYFGSMTKTAVRKFQSSKGIAVDGIVGKQTSKFLFESGTSSRSAASRTA
                     AASSKNAKIEMLDWWKEANKVFSIGTTAKVTDVGTGKSFKIVRTYGGNHADCEAATSE
                     DSKIIKSIWGGNWSWARRPIIIELNGRRLAASMAAMPHAGVDSQPANKILNKRSQGYG
                     RGYNLDKIKNNGMDGVFDVHFLNSRTHGTNRVDANHQKAVRKAASSEV"
     gene            15372..16829
                     /locus_tag="PKI50_07000"
     CDS             15372..16829
                     /locus_tag="PKI50_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNT03487.1"
                     /translation="MKNRWISFAAVTALIIIVLHLLFMYRIGQTGIPFNSTYRLQQIQ
                     RNIFWVIAISYTLVLLFAAAVIISINMDRRRYAESSLADNLKILEATVNATGDGILVV
                     DINRKILKANDLFFKIWNIPPDMCGLSDAREFLSFIRKQLKDSDVYEAWENSLYETCA
                     IEHYIASLHDGRIIDVFSNPLMDKGSLVGRVWSFRDITKQVNVEHELKKSYEEVKLIT
                     EALEYDKVKTEFFANISHEVKTPLNIIIGTLQLIELGLSNDNITADSDKMAKYTAIMR
                     QNCYRLLRTLNNLIYITEIDSGYIEMRLHNTDLAAIVKNITASVEKFLQSKEIQLELN
                     IEPDSLKIACDTDKIERILLNLISNSVKFSENGGKINISLYEKDKWAYISIKDNGIGI
                     PEEMKDLIFQRFRQVDKSFTRKREGSGIGLSLVKSLVEMHEGTIEVKSEYGKGSEFIV
                     KLPVRLAEDDEIAAAAEETSCVFVDRVNIEFSDIY"
     gene            16871..17824
                     /locus_tag="PKI50_07005"
     CDS             16871..17824
                     /locus_tag="PKI50_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422646.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNT03488.1"
                     /translation="MSKTWSGKRYHTLNHFLREKFGEKVFKISLDAGFTCPNRDGRVS
                     SGGCIFCSPRGSGDFAGTSQNIVEQFHEVRGMMNKKWKSGRYIAYFQAYTNTYAEADV
                     LREKYYSVLNLEDVAGIAIATRPDCLPADVLDLLAEISSRTYLWVELGLQTMHERTAR
                     LINRGYGLDTYIASVKELKKRNIEVVTHQILGLPGENKRDMIQTVDFISNTGTQGIKL
                     HLLHLLKDTRLAEMYRQGEFQLMSMEDYIEVVVDCIERIPENMVIHRITGDGPRDALI
                     APKWSLKKFEVINAIEHLMMDRDTWQGKKYEPNNDIMNIFV"
     misc_binding    18000..18246
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18288..20732
                     /gene="leuS"
                     /locus_tag="PKI50_07010"
     CDS             18288..20732
                     /gene="leuS"
                     /locus_tag="PKI50_07010"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNT03489.1"
                     /translation="MPYSKDIDRKWQKRWEETGLYKFNPQNADKKLYCLEMFSYPSGA
                     NLHVGHWYNYGPTDSWSRIKRMQGYEVFHPMGFDAFGLPAENYAIKTGIHPWDSTKNN
                     IATMEAQLREMGATFDWDYEVVTCDPSYYKWNQWLFLKLYENGLAYRKEAPVNWCPRC
                     NTVLANEQVVEGHCERCETEVIRRNLTQWFFKITAYAQELLDKLDEIDWPEKTKKIQR
                     NWIGRSEGSEIEFKIYRKDLSFKTFTTRIDTLMGVTYVVLAPEQELVDVITAPEQKEA
                     VEAYKDFARKASEIDRLSTTREKTGVFTGAYAVHPISGEKIPVWISDYVLATYGTGCV
                     MGVPAHDERDFEFAEKFGLPIPRVIKGVGDVDDKLPFVEYGVLVNSGKFDGMTSEDAL
                     VKITEVLAKEGRGELKINYRLRDWLVSRQRYWGTPIPIIHCEGCGVVPVPEEDLPVML
                     PYDVEFTPDGESPLKKSKEFMNTVCPKCGKPAQRDPDTLDTFVCSSWYQFRYPDNKNS
                     KEPFNKERINKLLPVDIYVGGAEHAAMHLLYARFITKALRDMGCLNFDEPFLSLVHQG
                     VILGADGEKMSKSRGNVVSPDKYINESGSDVFRTYLCFGFNYIEGGPWSDDGIKAIIR
                     FLGRAERMVERVYELRGQKSRNEVAEKEKELNYVRHMAIKGVTKDSEIFQFNTSIAKI
                     MELLNALYKYDQEVEYKNIPYFEEVLADLLKLLAPFAPHFTEEMWEKLGYEYSVFNQK
                     WPVWDESALVRDSVELAIQINGKVKGRIEVPSAASEKEIEDMAMANPETKGLIAGKQV
                     LRVIVVKGRLVNIVIK"
     gene            20967..22568
                     /locus_tag="PKI50_07015"
     CDS             20967..22568
                     /locus_tag="PKI50_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT03490.1"
                     /translation="MAGFLSGYVLVNVILGAALFMLRSNVLAVIILMLLIISSGIIFM
                     TRLKGIAVRIGDNIEVINKGQLNKNVRKMGIDMFDLISSRINDFLFKVRALIASFGVV
                     SKKVMKDAHEVEKQAEAIKYSSSEIATTIQSVAESVSKQAAYTHNMMEMVQNFAKDAE
                     DINANANTSFNVAKETKDIIEDSFRKFEDIRIKIQESKEYNKKVLQALDSLDEKLRAI
                     DTITEAVEGIASQTQLLALNAAIEAARAGEAGRGFAVVAGEVGKLADDSSQSAKEIKQ
                     LIDGITGQISELSVNIKDEADAIDKNMVYATEALSKSDIINDTLVSNMQAAGKITTLT
                     KTQLTSIDSIEKQIEMINDLTQQNAAVSEEIGASTQEQLATIETVHGNILQLKERLEE
                     SDSIISNFMKGFQLNDSIREKIKKTQELVKEITSRGDLFSSDKSSVQKYLNEQQSRYE
                     YVEFMALINSSGYYELATQDVDESLKDCSAKPYFTRAIQGEEYTSEEYISVITNNYNI
                     TVSMPVRQNGTIKGVLMADININEN"
     gene            22737..23600
                     /locus_tag="PKI50_07020"
     CDS             22737..23600
                     /locus_tag="PKI50_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNT03491.1"
                     /translation="MKPYKTNPMIAERIWGGRRLCSYNKNVGDKNAGESWETVSPEGE
                     IPVLIKLIDARETLSVQVHPDDEYAIRVENARNGKSEAWIVLECEEDSFIIYGFNRMV
                     QKKELRELLEMGLISGVLNYINVKKGDCIFVPAGTVHSIGSGILAYEVQQPSDLTYRI
                     YDWNRTDSRGKGRELHIDKALDVINYSSGLPCVFNVYDSLEDGIINIIECEYFALSFR
                     FINKEECHFYEPGKFRAITAVSGNLLLRYEDFQLSVKKGDTCIIPEEYDREVRVEGLG
                     AAEYIVAAGSN"
     gene            23686..23877
                     /locus_tag="PKI50_07025"
     CDS             23686..23877
                     /locus_tag="PKI50_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03492.1"
                     /translation="MTASIRLDNNEAKQTKQIIRFFKKGVKIRYNKKLTGKVRLYISE
                     KNSVKNISDILSLYENRLN"
     gene            24008..25606
                     /locus_tag="PKI50_07030"
     CDS             24008..25606
                     /locus_tag="PKI50_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT03493.1"
                     /translation="MGLLSLYLIINIVFGAALYLMRSSQLAAAALIILMTASGFVFIA
                     RLSSISGRIKHNIEEISKGQLNKNVKKTGIIMFDSIASATNEFLLKIRGLIGSFGDVS
                     KRIMKDAHGVEKQAEAIRYSSGEIASTIQSVAESVSNQAAYTHNMMEMVQDFTKGAED
                     ISENAETSLKIAKETKGTIEDSFGKFSDIKFKIQESKEYNKKVLEALDNLDNKIRAID
                     TITEAVESIAAQTQLLALNAAIEAARAGEAGRGFAVVAGEVGKLADDSSQSAKEIKQL
                     VDGITSQIGELSVNIKDEASAIDKNLKFAEEVLSKSDVINSTLTDNMKAAEKITMLTK
                     EQMKNIGSIEKEIQKVNDVTQQNAAVSEEIGASTEEQLATIEAVHGHIVMLLERLEES
                     NAIIGNFMKGFVLTDEVKEKVKETQTAVNELVKNEGILRMDKNAVEKLFREHQQKYNF
                     IEFMGLIDSKGYLTFSTVEVPENHRDASAKPYFIKACQGEAYVSEEYISVATNNYNIS
                     VTVPFQRGGIFGGLIIADININEN"
     gene            25635..27236
                     /locus_tag="PKI50_07035"
     CDS             25635..27236
                     /locus_tag="PKI50_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT03494.1"
                     /translation="MTGFLLGYTVINLILGTVLFMYRGNELAVILLIIAMAATGLLIL
                     AKLNSISSRIGNSIEEINKGQLNKNVKKTGIIMFDSIASAMNEFLLKIRSLIGSFDDV
                     SKRIMKDAHEVEKQAEAIRYSSGEIASTIQSVAESVSNQAAYTHNMMEMVQDFTKGAE
                     DISENAETSLKIAKETKGTIEDSFGKFSDIKFKIQESKEYNKKVLEALDNLDNKIRAI
                     DTITEAVESIAAQTQLLALNAAIEAARAGEAGRGFAVVAGEVGKLADDSSQSAKEIKQ
                     LVDGITSQIGELSVNIKDEASAIDKNLKFAEEVLSKSDVINSTLTDNMKAAEKITMLT
                     KEQMKNIGSIEKEIQKVNDVTQQNAAVSEEIGASTEEQLATIEAVHGHIVMLLERLEE
                     SNAIIGNFMKGFVLTDAIKEKINKTQELLREIIQKENIMALDKNSAERYIKEQQKKLE
                     YIELIVIIDNRGYITGATTELPEFVRDCSSKPYFTNAMKGNTYVSKEYISCASGNYNI
                     SVSMPIIVNGELKGVINADININEN"
     gene            27299..27592
                     /locus_tag="PKI50_07040"
     CDS             27299..27592
                     /locus_tag="PKI50_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03495.1"
                     /translation="MHKYVKRLKRRAESGDESSKDKYEEIMECLNTMDDDRVANCIIL
                     MKKHIPELWNQSALLLKKYIIEESRSLEFLSKAEVELIEFLRDYLRDEGDDRH"
     gene            complement(27619..28677)
                     /locus_tag="PKI50_07045"
     CDS             complement(27619..28677)
                     /locus_tag="PKI50_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNT03496.1"
                     /translation="MILDAKLRLTALALLSVLLLSGCQKPFPSASAKPLSKTGFLLGT
                     IVEITVYDKKDEALLDKAFERIQEIEGRMTINNAENSEIISLNQASGKNEVKLSPDTY
                     YVVERGKEYSHKSGGLFDITIGPVVKLWNIGTDYAAVPEKNKLEEAAGLVDYSKLSLN
                     PGNHTAFLEEPGMQVDLGSIAKGYAADEAARILKESGVEHAIINLGGNVITIGGNPNG
                     NPWRIGIQTPSNPRGDFLGIVQIQDQAVVTSGTYERYFEENGKIYHHILNPFTGYPVE
                     NNLSSVSIITGKSIDGDGISTTVLLMGLEEGMKLVESLENVEAVFITNDKGVYISSGL
                     KNNFKITNSDYKYRNNGE"
     gene            28908..29291
                     /locus_tag="PKI50_07050"
     CDS             28908..29291
                     /locus_tag="PKI50_07050"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNT03497.1"
                     /translation="MKIKKGDLIIIVILVAALISWLVINNPGKPEDERLIVIETNGDI
                     YREIPVETGMEQQEIHIQFENGKYIDIIVDENGAYVKDVICPDKVCQKTGLVSRVGQS
                     IVCLPNRVVVYIEGRAESEIDGISF"
     gene            29312..29839
                     /locus_tag="PKI50_07055"
     CDS             29312..29839
                     /locus_tag="PKI50_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNT03498.1"
                     /translation="MSRTKKMVLLGILVSQALILNLVERLIPVPVPVPGIKLGLANAI
                     SLIAIILFGPKEALAVVGMRTFLGSVFGGGISSFIYSFIGGTISTMAMAIMYRKFRTL
                     FSLPSISVIGAVFHNIGQILVASLIIENAKLFYYLPALLISAVITGLFIGFAVQYTLK
                     PMSHVLGIGSDTNVE"
     gene            29829..30782
                     /locus_tag="PKI50_07060"
     CDS             29829..30782
                     /locus_tag="PKI50_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT03499.1"
                     /translation="MWNDNSFLKKEMDAVEDTIKNSLRTRNKTVKEALVRFAESGGKR
                     LRPAFTILGGTFGQYDSNTIVPIAAALEIIHMATLIHDDIIDDAKLRRGNETVHSVLG
                     KDVAVYSGDFLLTRAFMLVADYADIDMLKQIAKASAYICDSEIAQNEQKFDTNVSVKQ
                     YLKRIGGKTAALFGISLYMGAYKAGCPRKLVNRLGVLGNNIGMAFQIVDDILDFTGNQ
                     AKVGKPLLRDAAQGIYTLPVLYALHSEYREEAIKAINKTQEEKGANLYEVLDASKAIE
                     RSKNIAEKFIGKAMKIAGELPESIGRDIIMEIIDEQLERKF"
     gene            30968..31753
                     /locus_tag="PKI50_07065"
     CDS             30968..31753
                     /locus_tag="PKI50_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNT03500.1"
                     /translation="MNIIQAIILGLVQGLTEFLPISSSGHLVLFQKIFGINEPTLVFD
                     TMVHVGTLAAVMTVLRKEILSLLKRPFQRLTWLLIAGTIPTGIIGIAFKDSFEAMFQS
                     GSTLGFEFLATGIIILFAGRLNNGGKHIEETTFLDAAFIGILQGAAIMPAISRSGLTI
                     SGALMRNLDREFAARFSFLLSIPAILGAALFQAKDILEAGSSGIINAPIVAGTIAAAA
                     AGYFSVRFMIMLVKKGSMKYFSYYVFIIGALVIIDQYITHLFF"
BASE COUNT         9932 a         5743 c         7608 g         8471 t
ORIGIN      
        1 gataaaatac catccatgac aaattatatc aaaaattggc atatttatat agtgtaaata
       61 atgttaatta tttagcatac ttacaatatt ttgatattag tcatggaagt tttttgctaa
      121 attattgcac tgcccttatt aatttcttga gtttgtgcta aaatatgaga taacgaggtg
      181 attgtatgaa ctcaaagaat aaaaaggaag tccagggtgt tggggaactc acccacataa
      241 atgctaaggg ctatgccaag atggtggatg tgtctgaaaa ggaagaaagt gtaagagagg
      301 caattgcaag aggttctgtt tatatgaaaa gggagacctt gaaggccatt gcagagggaa
      361 ggataagaaa gggagatgtc ttatccgtag cacaggtagg cggtataatg ggagccaagt
      421 ctacaccgga tataatacct atgtgccata atgtcagcat atatggtgca gatattgaat
      481 ttcaggctga ttatgaaaat tccaaaattg atattgaagc ggttgtaaag actgtcggag
      541 ttacgggagt tgaaatggaa gccttgacgg cagtatccgt tgcggcgttg actatatatg
      601 atatgtgcaa ggccattgat aaattaatgg tgataagtga tatacatctt gtacataaga
      661 ggggcggcaa gtcaggggaa ttcaatttca gcggtaagtt gaacgaagac atcaataata
      721 cgaaatgcta gtgcaggagg taggatacca tggagggatt cagcgtagga atcataacag
      781 taagcgataa aggttcaaag ggcgaaagag aagatgcttc aggccctgct attgaagaga
      841 ttatgaagca gataggaggc cgtgtagaaa aatatgttat tgtccctgat gaaatgcggg
      901 atatcaagga agctatcata gacatgtccg atactctcgg attgaattta atactgacta
      961 ccggaggcac gggcttttca aaaagagaca taactcccga agcaactctt gaggtaattg
     1021 aaaggtatgt accggggata cccgaggcaa tgagggcgaa gagccttcag gctaccccga
     1081 aggcaatgct ctccagggca caggcgggca taaggaagca gtccctaatt attaaccttc
     1141 ccggcagccc aaagggtgtg agagagaatc ttgaagcaat aatacccgca ttgaagcatg
     1201 ggatagagat attgacgggc caggcatcgg agtgcggcgg caaatgaggc gtttatttca
     1261 agaataagct ttctattaag tcgactgctt cttttatctc cgagaaatcg aactggggta
     1321 cacctttgtt taaaggcgta tttgtgatga tggcaaacaa gtcttcatct tttgaccata
     1381 tttcattcgg gaactctttc ctgtaaactt ccagttttgg tttgcgctcc tgcttaaaac
     1441 cttccgttat gattatatct acattgctta tcttgcttat cacttcgtcc aggctgtatt
     1501 ctttttcaac ttcttcaatc atcgctaatt tttttggaga tgatataact acgaaatctg
     1561 aacctgcttg tgcatgtttc catgtgtctt tacccggatg gtcaatatca aagccgtgaa
     1621 catcatgttt tatggtacaa atcctgtatc ccctggattt taattctctg acaatatcgc
     1681 ataatactgt ggtctttcct gtttttgaat tgtttgcaac aactgaaaac accggtatca
     1741 taattccgcc tccagatggt tgatcaattc aacagttacc cggtctcctt ttttataagg
     1801 gcttgatccg ccggggataa gcagtaatga gtttttccct gacaggctgg ataaaactcc
     1861 cgagctgtgt ttatccggaa gtacggtata ataggctcct tcttttttgt atgtgaaggc
     1921 ccttaaatac ctgtcctgct cgctgacttt attgaagtcg ctttccagta tggattcaac
     1981 tttcatatat ttgtgctttg gttttcccat taatcttaat attgcaggcc ccacaaaaat
     2041 ttcaaaggtg ataaatgctg cagccggatt gccggataat ccgaataaca atttattccc
     2101 atgctttgcc gctactattg gggttccagg cttcattctt gttttccaga ataatatatc
     2161 ggctcccgct gccttgaagg cttctttgat tatatcggca tctccgacag aagctcctcc
     2221 ggttgtgatt atcatatcag cccatttgag agcagaatcc agtttcttct taacatcgtt
     2281 taccctatcc atacaaattc cgagtatttt ggcatttccg ccgtattttt ttacctgtgc
     2341 cgctattaca taggaattgc tgtttcttat cttgccgtca gccaattctt catcaatttc
     2401 aagaagctcg tcgccggttg acagtattgc aattgtaggt ttggagtata ctttaacatg
     2461 ggtctttccg tttgaagcca gtacacctat ctctgcaggc ccgatggtta tgcctttgcg
     2521 cagtatcagg tctcctgccc gcatatcttc ccctgctttt actatattgg attggggctt
     2581 gagatatctg aatattttaa ctgtcttatc ggtaaattca gtatcttcat atcgtataac
     2641 cacatccgcg ccttccggta ttttggctcc ggtcataatt cggattgcct gcccgttaca
     2701 tatttttttt ctgcttacat atcccgcttg tacatagtct attacttcca aggttaccgg
     2761 agagttttcg gctgcattta ctgtgtcttc cgatttatag gcatagccgt ccaggggtga
     2821 tctgtcaaag ggaggcatgt cggtttcgga tacaatgtct tcggcaagga catgatccaa
     2881 gctgtcaaga atgtacagtt cgctttgctt aatatcaatt ttttcttccg ataatattct
     2941 ttttgcagtc tcaacggtta tattttctaa catttttcct cctcaatgct taccgaaatt
     3001 gcagtaaggg aatttacaca cctcacagga taagcattgt cccccgtacc ccattctggt
     3061 gatatctttt ctgataattt tttcgcctgc aaagactctg ggaagcaata ggtcaaaggc
     3121 tgtagtatgt gcaaacatta cacagccggg aagcccaaac acaggcgtac cgtccaggta
     3181 ggcaaacagc agcattgcac cgggtaaaac agaagttccg taggttataa tatctgctcc
     3241 ggttgcttta atggcgccgg gagttttgtc atccggatcc actgacattc cccccgtaac
     3301 tacaatgagt tccgcacctg ctttttttgc atccaatacg gcattcttta tttcatccaa
     3361 ttcatcggtt actatggttt tattaaaaat tcccgaaccg tagtgtttta ctttcttttc
     3421 aacaaccggc ccgaattcat ccttgatcct tcctttgtat acttcagagc cggttactat
     3481 caaagctgtc ctgagattta tgaaagactt gattcggaac atcggatcct tgccttttac
     3541 catgttctcc acatgttcaa ttttctcttt ttttattgaa aggggaataa tcctgcatcc
     3601 tcccagcaat gcatccttct ccacacatat attctcttgt atggtagcca aacagatatc
     3661 ttccgtatca ttaacctgct ccagcagatc cctgtttatt ttcaaaagcc ctctatacat
     3721 tgattttaaa ctggtttttc cttcagaggg ttctgaaaaa ctgatgccgg gtcctgcaaa
     3781 cagttttccc aacctgagag ccgcatcatt ttcatggagt tcgtcctcac caagttcaaa
     3841 aacatataca tgctcctttc ctattcgcag aagccgttct atgtcatttt ccgttattat
     3901 atgtcccttc ttgaaggcag ctcctttgta ctccccgggt atgatttcgg taatgtcatg
     3961 tcccagtacc attcccacag cgttttctgt ttttactgta ttcaatatac caccccaaca
     4021 ttattttatt ttacgaataa attatataca attaacacaa tgttttcaaa tttcaatgat
     4081 cgggaaaata ctttttaaat atagtattta ttgcttcttc acattcatta taaaacagtt
     4141 cgtatgcaga caccaattct tccgcctcta tagtaagcag ggagattcct cctcctgcac
     4201 ctcccgtctt actgtacaga agccggaagc ccaacctgat ttctatcttt tttatcagat
     4261 tatgtgcctt gttataggac atattcatgg cctttgcagc tcctgccaaa gatccctttt
     4321 cctttacgcc tttaagaagc aagtaagaac ccgtcccaaa tataacacca tttttgtcca
     4381 gccataattt atattttata ttgtacatta cttaaatcat tcctttcaga ataaattcac
     4441 actattatta taacacgaag atattacaaa atttgcttta cttttcatat taattacgtt
     4501 atactcataa tagtataacg aatgttaagg agatgtcact ggcgaaaaag atgcaaagct
     4561 cttaggagac agcattattt tgttaaaaga ggtaaagttt atacactgaa aaatgaatat
     4621 ataaaagcat agaaacacag tatgggctgg aagtcagtgc agctctattt gtgttttttg
     4681 ctattgataa aaagttgaca ataggtttga actcttatat aattaatttg ttacttatat
     4741 aacaatgatt ctgatatatt aatgcagtat tattataatg ttggaggtgg tgaaatggtt
     4801 acatttaccc tgaatgggaa agtagtaacg gctaaagagg ggactaccat tttggatgcc
     4861 gcgaaagaaa acggtgtaga tatccctcat ttatgctatg ataaaaacct ttctgcattt
     4921 ggtggatgta gactatgcgt ggtagaggtt cagggggcaa aggcccttgt agcttcatgt
     4981 accaataccg taagtgaggg tatggtaatt cagacagagt ctgaaactgt agtgaatgtt
     5041 cgtaaggatc ttcttgattt gcttttggca agtcaccctg aggattgcct gacttgtgaa
     5101 caggctggca attgcaagct tcaggactat gcatacagat atggaattaa gaggtcctcc
     5161 tataaaggag aaaaaaagag ctatgatatt gaaagcttga atccggtaat ggaaagggat
     5221 caaagcaaat gtatcttatg cggaaaatgt gtcagagtat gcagggaagt tcaggttaca
     5281 ggcactatag actttatcaa cagaggtttt gagaccaaga tagcaacagc atatgatgtc
     5341 ccgataagta ctgaaaactg tagattatgc ggtcagtgca tcagtgtatg cccgaccggt
     5401 gcaataataa acaaacagtt gaagggcaca agaccgtggg aagtgagcaa agtacgtact
     5461 acatgcccct tctgcggtgt aggatgcaat tttgacctga atataaagga cggaaaggtg
     5521 gtaggggtta cacccaatcc agattctccg gttaacggaa cctccatttg cgtaaaaggc
     5581 agataccata ttgacatgat aaacagcccc gaaaggctta caaagccttt ggcaaagaaa
     5641 aacggggaat ttgttgaaat aagctgggat gaagcattac acacagtaac agaaaagctt
     5701 aaggagacaa aggaaaaata cggccccgat gcaatagcag gcttgagttc tgcacgctgc
     5761 acaaatgaag agaatttctt gttccagaaa atgatgaggg tggcagtggg tacaaacagt
     5821 gttgaccact gcgcaaggac ctgacatgct ccgacagttg caggtcttgc agctacatta
     5881 ggaagcggag ccatgacaaa cgccatttcc gaagttgaag gaaatgaagt attgttcatt
     5941 ataggttcaa atgctacaga agctcatcct attataggga ataagatgaa gagagccgcc
     6001 aggaacggat caaagcttat agtcatagat ccgcgcagaa cagagcttgc ggaatatgct
     6061 acactatggc tgccgttgaa ttccggaacg gatgttgcgt taataaacgg attgatgcac
     6121 ataatcgtaa aagagggatg gcatgataag gaattcattg aaacccgctg tgagggattt
     6181 gatgaattac tcaaggcaat agaaaaatat actccggagt atgtgtcaaa gataacggga
     6241 atctctgaag aattgcttta tgaaacagca aagctgtaca ccagcacaaa gaaagcaggt
     6301 atattctaca cattaggaat cactgagcat ataaccggaa cagataatgt tatgagcctg
     6361 gcaaacctgg ctatgcttgt gggacatctc ggtatagaaa atgccggcat taatcctata
     6421 cgcggacaga ataacgttca gggtgcttgc gatatgggag cgctgcctaa cgtattctca
     6481 gggtatcaga gtgttactga tgaaaaggct atccagttct tcgaggattt ctggaagttc
     6541 aagctcaata ggaacacagg ccttaagata cccgaaatgt ttgacggatc ccatgaagga
     6601 aaagtaaaag caatgtacat tatgggagaa gatccggtac ttacagaccc tgatgcaaac
     6661 catgtaagaa aggcattgga aaagcttgac ttcttagtag ttcaggatat cttcctgtcc
     6721 gagactgcca agcttgcgga tatagtactt cctgcggcat gctatgctga gaaggaagga
     6781 acatttacca ataccgaaag aagagtacaa atggtcagaa aagcagtcga agctcccgga
     6841 gaagccagac cggattggga gataatctgc gatgtggcaa aggggcttgg cgccggtgga
     6901 tttgactaca aggatgccga agagatattc aaggagatag cggcaactac acccagctac
     6961 agaggaatga cttttgcaag actggataag aacggattgc agtggccatg tcctactgaa
     7021 gatcatccgg gtactcaata cctccataaa ggtacattcc ccaaaggaaa ggcattaatg
     7081 aaggcaattg aatataaagt cccggcagaa gccgtagatg aagagtatcc tatacttctc
     7141 agcacaggca ggatgctgta tcattacaat attacctcaa gattttctga aaagctggat
     7201 gccataaaac cttacgaact ggctgaaatt aacccggcgg atgctgaaaa gctcggactt
     7261 aaagaagaag acttcgtaag ggtgacctcc agaagaggtt ctatagtaac cagagtgaca
     7321 ataaccaata aagtaaaacc cggtatgatg tccatgacat tccattacag agaatctccg
     7381 gcaaacgaac tgaccaattc ggcttttgac ccggtaacca agacaggaga atacaaggta
     7441 tctgctgtta aggtggaaaa ggttaaagat atgcagggga cagcagtata atataattta
     7501 gttttcaagg gatgatttcc gtattcggag tcatcccttt ttcattgcat aaaagcatca
     7561 ggtattgtac aataagcggt gaaagcaaag gcataactta ttttttgggg gtatataatg
     7621 agcttggtaa atacggctgt aatacttgca gggggtaaaa gcacccgtat gggttttgat
     7681 aagcagctta taagggttaa ggatatctgt atagttgaac atttggttga aatgctgagc
     7741 cccgtgttta aaaacataat tgttgtaacg aacaagcctg agctgtataa agacaagaat
     7801 atagccatag ctgaggatgt atataaagga tatgggccta ttgggggaat tcatgcgggg
     7861 ctttccaaat cacagagcat gtataattat tttattgcct gtgacatgcc taatattaat
     7921 aaatgctaca tagaatatat gataaagagg ttggaagagg attcttttaa gagtgatgcg
     7981 gtaatcacga aaaaagcgga ttggatagaa ccctttaatg ctttttacag taaaaagctt
     8041 atacccgcta ttgaagataa tattaaaaag ggcaaggtaa gaacaatgga gcttttggaa
     8101 atctctaatg ttttatatat tgatgaagct attgcacggg gttttagccc cgactggagc
     8161 atgttcgcaa atttgaacac ccgggaagat ttgaaagggc ttgtttgact ggcaaagcag
     8221 caattatatg gaaaataaat ccaaacaatg ttaaaccatt gacaatgttg tttgtgttgt
     8281 gtatcatata ttatagataa aacaataaca taaacttaat atacacattt ctccgagtcc
     8341 aaatttccta aggcatatgg ctatggaatt tggaccgata gggtaccgct agaaatgtcg
     8401 gtgcctccct tttggaaagg agcagtggtt aatgggtatg cccgtatttg agcatgcgta
     8461 ttaatgtatc aaactccttc cggaacggaa aggagttttt ttgtatagaa agtattattg
     8521 ctccggcaag cagagctgcc tttgccggaa aaaatttagc ggaggtataa tacaatggaa
     8581 acggtcaata ttacaatcga cggtattaaa acccaggttc ccaaagaatt cacagtactt
     8641 gaagctgcaa aatctctgga tatcaatata cctacccttt gccatcttaa agggatcaat
     8701 gaggttggag cctgcagaat ctgtgtcgtt gaggtaaaag gagcaagaac acttcaggct
     8761 tcttgcgtac tgcctgtatc tgagggaatg gaggtcaaaa catgttcccc tgcagttatg
     8821 gaggcaagaa aaacaatact ggatctaatt ctctcaagac atgaaagaga atgtactact
     8881 tgctcaagga atctgaactg cgagctacag aagctctgtg atgaaatggg aatagacaag
     8941 gtttcctatg aaggggagaa aatagtatat gctgtggatc aagcgtctcc ttccatagta
     9001 agagatcaga ataaatgcgt attatgtgga agatgtatag ccatatgcag ggatatacag
     9061 catacagagg ctatcaattt tgccaataga ggaatcagaa cgacagttac tgcacctaat
     9121 ggaaaagcgc ttacagaagt gaattgtata aactgcggcc agtgcataaa gatctgccct
     9181 gtgggcgctc taagggagaa ggatgatact cataaggtgt ggagtgcttt gggtaatccc
     9241 gatttgcatg tcgttgtgca gactgccccg gcagtcaggg tcggcctcgg agaagagttc
     9301 ggtatgccgg tggggacaaa tgtcgaaggc aagatgataa cagctcttaa aagattgggt
     9361 tttgacaagg tgtttgacac ggatttttcc gcagacctta caatacttga agaaggtacc
     9421 gagttgctga acaggattaa gaacggaggt aagctccctc ttatcacatc ctgttcaccg
     9481 ggttggataa aattctgcga acataattat catgagttcc tggacaatct ttccacttgc
     9541 aagtcacctc agcagatgat gggtgcagta gcaaaatcct actacgcgga gaaagcaggc
     9601 atagatccga aaaatatttt tgttgtatcg gttatgccgt gcactgccaa gaaatttgaa
     9661 gcaaacaggg aagaaatggc tttagacgga ttgaaggatg ttgatgtggt tcttacaaca
     9721 agagagcttg cgaagatgat aaaacaggtt aggattaatt ttacggcttt gagtgaaggg
     9781 aaatatgaca gtatattggg tgattcaaca ggtgccgccg taatcttcgg agctacagga
     9841 ggggttatgg aagcggctct gagaacggtt gcggatatat tgaccgggca ggatctcaaa
     9901 aagatcgatt atacacccgt aaggggaatg gaaggaataa aagaagcgtc agtgaacgtt
     9961 gacggagttg atataaaagt tgcggttgcc catggtacag ggaacgctgc taagcttttg
    10021 gacagcataa aggacggtaa ggccgaatat catttcatcg aagtaatggc ttgccccgga
    10081 ggatgcatca acggaggagg ccagcccata ctgttggata aggaaaagac tgaggaagtt
    10141 aagaagctta gagcgcaagg cttgtataag attgacaagg aagacaggag gttcaggaaa
    10201 tcccatgaga atcctgaagt gaaaaagctc tatgaagatt atctggagga acccaacagc
    10261 catatatccc atcgtttgct gcatacaaag tatattgaaa gggacagggc ttaggataaa
    10321 tgaaagggaa gctcaatctg ctaacaaagg cagcagtatt actggctata gctatagtat
    10381 tccagcttgt caaaatgggc cagtatgtaa cgggaacagg aatcaatgcg gtacttatta
    10441 ctgcagttgg agtttgcgga ttgccctggg cggcggcaat cggtataatg acacccatgc
    10501 tggcggtgct tcttggggtg cagccgcctc cgacaattgt gctggttccg tttataatgg
    10561 cggggaatac tgtatatgtt gttttgttca gactattaag aagatataat gattatatcg
    10621 gtataattgc ggcagctttg acaaaataca ttttgcttta tacagcggca aatataatag
    10681 tagacaagct gcctgcgccc ataaggctcg ccttcagctt tccgcagctg ataactgcag
    10741 cagcgggagg agcaatagca ttaatagtat taaaaattgt acaaaaaaag tagtgacgcc
    10801 atggcgtccg gataaggctg attttagtgg atcccgctaa aatcagcctt ttatttttta
    10861 tgtggaaggc aagtaataaa accaaatagg agtaagtata tttatatata aacgaatggg
    10921 ggattcaagg gggagttcaa tgagagatat tatggcagac aagttgacga aagcagtaat
    10981 agggctaatg ttattggtga tgctgctgtt cgtatgcctg gagaagcctg caggggcaag
    11041 aaaggttttt tcgggattaa gtgaaacaga ggacatgacc gattccatac atcagatatt
    11101 ccgaaacaga aataatgcga taataaacgg tgaccttaag ctgattcaat ccctgtatga
    11161 cacaagcaca aagtacggta catgggcaca tcagcatgag ctgaccaagg tcaagtacct
    11221 ccacaagtgg gagaagaagc agggggtgaa attcaccgaa atcataccga cggtgaaggt
    11281 tagaagcatc aagccgaagg gcgaaaaata cacaattaat ctgatgtgtt caacggaata
    11341 taagtatgta tatgaaaatg agcccggtgt tgtaaacagc ttcaggatag gaacctacca
    11401 tgtgcttgac ctgctgtaca aggatgaggt ctggataata acaagggaat ggtataagga
    11461 cccctttgaa gactccttga atctgggaag gattgacgtt gattcaatac atcagtacat
    11521 aagcacccat gctgcaaggg atttttccaa tataggggac aggcggaaaa atgcagtagc
    11581 ttatgcagat aaatactgcg gcgctgccag cgaggagaaa tatggctaca agtacaacaa
    11641 gaaatacagg aactacaact cccagggagg cgactgcgca aattttgcat cacaaatact
    11701 tcacgaagga gggaagttca gaaaaaccca ctcgtggaat tacaacaata agggggctac
    11761 ccgtgcgtgg gtcaacgcag acggcttcaa gagctatatg ctatacagcg gcagggcatc
    11821 actcattgcc tacggtaatt atgaaaaggt gtataaggca tcctacaagc tattgcccgg
    11881 ggacttcatt gcctatgaga aaaagggaga tatcacgcat atttcagtgg ttacaggcgc
    11941 cgactcaaaa ggatacgcac tggtaacctg ccataatacc gacagaaaca gagtgccctg
    12001 ggacctgggc tggagcaacg gcaagatcaa gttctggctg atacgggtac atttttaaga
    12061 atgtcgaaaa agcacgagcg aaagttgctg aataagcagt ttataatgct tcttatttat
    12121 ttagcgggcg tgacagagta gaagaagaat attgtatatg ctcatataaa cagctgcaaa
    12181 tccgattcca gtgcatcctt gagataatcc tttgcttcca ttatctcaag ctcggaaatc
    12241 atttcctcct gtttaaggcc cattatctcc attgaaagct tgcagccgta gaatttcaca
    12301 tcctttttcc ttgctccctt caggaagtca ataagatgag gagcactgtc gtcatccatc
    12361 atttcaagga gcattttctt gccaaggcct gccatattca tacgggagag gggcatctgc
    12421 tcaggccctt ttggggtaac tgccccgaat attttttcat aggctgtctt gtcttttgcg
    12481 gagaactttt caggatccct caccaggaag agcccccaaa aagcaaagaa catggtaact
    12541 tccacctgta tttctcttgc cgtattggca agtataagtg ctgccatagc cttgtcgtac
    12601 tcaccgctga acataagtat gttcattctt ttgttcaatt aataaccctc cctgtaccaa
    12661 ttcggatatg cgtattttat ccataagaca gggaagtaat gcataaaaga gtaaatcaca
    12721 gcatgtggaa tgcccgctct taaatagcat tgataatact gaaaataccg gatacctttc
    12781 aaatgaattt gataatagcc atcagtgtgt taagtgctgc ccgtatcttt ctttatacag
    12841 ttccttattc gccgatattt catacagttt attcacaagc acatctatct catcgtaatt
    12901 gttgtagagc ccaaagctta gcctgaccat tccgggatga ggaagagaag ggttctttat
    12961 ccgctttctt atatcatttc tgggtatttt cataagcctc tgcacatatg gctgggagca
    13021 gaagcagccg ttccttaccg caatacctga ctcagcggat aaagcgtcgg ctacaatctg
    13081 atggtgcagc cccgcaatat taaaaggtat aataccgact ctgtcccttg ggtcatcatc
    13141 acaatacagc ttaatatcag gaataattga aattctgtca agggcatatt caaggaggct
    13201 gctctcgtag gcctccaggt tttccattcc aacagaagca agtgtcctga ctgcttcggc
    13261 aagagccaca acgcccatta tattaggagt gcctgcttct tccttgtggg gaggctccgc
    13321 ccattttatc aggtcatggg tgacgatgtt gatggtacct ccgcccctgt attcaggctc
    13381 ccccttttca aaatcagact ggtggccgat aagcactccc gttccaaagg gcgcatacat
    13441 cttatgggcg gagaatgcca gataatcgat atgccggctg cttccgtcag gcttcatgtc
    13501 aacgcaggaa tggcctataa gctgggccgc atctaccata agctttgaat catatttgtg
    13561 caccaattct gcgatatcat gtatgggatt tttatagcct gtgacgttgg aagcgcctgt
    13621 taccgtaaca agagcaagat ttttccggta cttgtgcagt tttctttcaa ggtcccgcat
    13681 tgagagcctt ccattgttgt ccacattgat atagtccacc aaatatttgt ttctccaggg
    13741 caggtcattg gaatgatgct ccatgcaagt agatagtatc acacttcgcc tgtttttgtg
    13801 ataaagcctg taagacagct tgttgatggc ctcagaggta ttcttcacaa aaacaaccag
    13861 gctgcccgga tcggccttga caaatttttt tattgtctcc ctggaatttt cgtatatttc
    13921 tgaagagtat ttggatttaa agcctgttcc cctgtgtatc gaagaatacc agggagcaaa
    13981 gtcctctatt gcctgaatta cagatgcgaa gggcggagtt gttgcagcgt tgtcaaaatt
    14041 tatggaagcg gctatttgac cgttggaaag cggtatcccg gtatccgcac cgacaataag
    14101 gtctctgtaa tttgcattgt tttttacaag catccaatca cccgccccaa ataatatata
    14161 tattcattct attcaataca ggggaatatg tgtgtatggc acataattcc attgaaaaaa
    14221 tggtatttat tatgtaaact taaaatacgc cgtactgcaa taggtgctac atattcacct
    14281 cgtaagccgg gagaggagag taagtgagaa ggagcaagct tttaattttt tttctggtca
    14341 taactttatc cttaacatcg gtaacctttg cagatacgtt gaagtatggg agcagaggag
    14401 ccgaggtgac caagcttcag gaacagctga aaaagctggg ttattttaac ggcaaagcaa
    14461 cgggctattt tggaagtatg acaaaaacag cggtcagaaa gtttcagtca agtaaaggta
    14521 tcgcagttga cggtattgta ggaaaacaaa catccaaatt tttgtttgaa tccgggacaa
    14581 gcagcaggag tgcagcttca aggacagcgg ccgcatcaag caaaaatgca aaaattgaaa
    14641 tgctggattg gtggaaggaa gccaataagg tttttagtat cggaactact gcaaaggtta
    14701 cggacgtagg gacaggcaag agctttaaga tagtgaggac ctacgggggc aaccatgccg
    14761 actgcgaggc agccacctca gaggattcaa aaattataaa aagcatttgg gggggcaatt
    14821 ggagctgggc cagaaggcca attattattg aattaaacgg aagaaggctg gctgcttcta
    14881 tggcagcaat gccccatgcg ggtgttgaca gtcagccggc aaacaagatt ctgaacaaga
    14941 gaagtcaggg atacggacgc ggatacaatc ttgacaagat taaaaacaac ggtatggacg
    15001 gtgtgtttga tgtgcatttt ctgaacagca ggacccatgg aaccaacagg gttgatgcta
    15061 atcatcagaa ggctgtcagg aaagcagctt catcagaggt ataaatggac ggaatataaa
    15121 tattgcagta cggtaaaggg gtgccgcaaa ctaccggatg agtagatggc gggatccctt
    15181 tttcttcaga aaatatacgg cggaataagt cgaattcact gtgaaaattt atgtcgtata
    15241 ttgtaatacg aagttgattg aaataaattt tgtactatga aataatagtg ttattgatat
    15301 gttgcataca agtgacaaaa attaacatgt tatgtataat atttctactt ttcttatccg
    15361 gggggtcaga attgaaaaac agatggattt cttttgcggc agtgacggct ttaataatta
    15421 ttgtattaca tttattattt atgtacagga tcgggcagac agggatccct ttcaatagta
    15481 catacagact gcagcagata caaagaaaca tattttgggt gattgcaatt tcatatacac
    15541 tagtattgct gtttgcggcc gcagtaatta tatcaatcaa tatggacaga agaaggtatg
    15601 cggaatcatc cttggcggat aaccttaaaa tactggaggc caccgttaat gcaacggggg
    15661 acggcatact ggtagttgac atcaaccgaa agattttgaa agcaaatgac ctgtttttta
    15721 aaatatggaa tattcctccc gatatgtgcg gcttgagtga tgcgagagag tttttaagtt
    15781 ttatcaggaa gcagctaaag gattctgatg tttatgaagc ctgggaaaat tctttatatg
    15841 aaacctgtgc aatagaacac tatattgctt ccctgcatga cggaagaata attgacgtat
    15901 tttcaaatcc tctgatggat aagggcagcc tggttggaag agtatggagc ttcagggata
    15961 ttacaaaaca agtaaacgtt gagcatgagc tgaagaagag ttatgaagag gtgaagctca
    16021 tcacagaagc cctggaatac gacaaagtca aaaccgagtt ctttgccaac atttcccacg
    16081 aagtcaaaac acctcttaat attatcatcg ggacactgca gcttattgag cttggcttga
    16141 gcaatgacaa catcactgcg gacagcgata agatggcaaa atatactgca attatgaggc
    16201 agaattgcta caggctgtta agaacgctta acaatttgat atatattacg gaaatcgatt
    16261 cgggttatat tgagatgcgt cttcataaca ccgatcttgc ggcaattgta aaaaatatta
    16321 cagcctccgt tgaaaagttc ctgcaaagca aagagataca gcttgaactg aacattgaac
    16381 cggatagcct gaagatagcc tgcgatactg ataaaatcga gagaatactt ctgaatctta
    16441 tttcaaattc tgtaaaattt tcggaaaatg ggggtaagat aaatatatca ttatacgaga
    16501 aggataagtg ggcatatata tctataaaag ataacggcat cggcataccc gaggaaatga
    16561 aggacttaat tttccaaagg ttcagacagg tagacaagtc atttacccgt aagcgcgagg
    16621 gcagcggaat aggcctttct ctggtgaaat ctcttgtgga aatgcatgaa ggaacaatag
    16681 aagtaaaaag cgagtacggc aaaggcagcg agtttattgt caaactcccc gtaaggttgg
    16741 cggaggacga tgaaatagcg gctgcagccg aagaaacatc ctgtgttttt gtagacaggg
    16801 tcaacataga attctcggat atctattaga ttatcaggca gaacaaatca ggtaaaaggg
    16861 tgaaggaaaa gtgagcaaaa catggagcgg aaagagatac cacaccttga atcacttcct
    16921 cagggaaaag tttggggaga aggtatttaa aatatcattg gatgcaggtt ttacatgccc
    16981 caatagagac gggagagtgt cttcgggggg ctgtattttt tgcagcccga gaggatccgg
    17041 agactttgca gggacttctc agaatattgt tgagcaattc catgaagtca ggggtatgat
    17101 gaacaagaag tggaaatccg gcagatatat cgcatatttt caggcgtata ccaatactta
    17161 tgcagaagca gacgtattaa gggagaaata ttactcggtg cttaatctgg aggatgtggc
    17221 aggcattgcc attgcaacca ggccggattg cctgcctgcg gacgtattgg atttacttgc
    17281 ggagatcagc agcagaactt atctttgggt tgaactgggg ctgcagacaa tgcatgagcg
    17341 gacagcccgg cttatcaaca ggggatatgg gctggataca tatatagctt ccgtaaaaga
    17401 acttaagaag cggaacatag aggtggtaac ccaccaaata cttggtttgc cgggtgagaa
    17461 taagagggat atgattcaga cagttgattt tatctccaat accggaaccc agggtataaa
    17521 gcttcatctg cttcaccttc ttaaggatac caggcttgcg gaaatgtaca ggcaagggga
    17581 gtttcaactg atgtccatgg aggattacat agaggttgta gttgactgta ttgaaagaat
    17641 tccggagaat atggtgatac acaggataac cggtgatggg ccaagagatg cgcttattgc
    17701 tccgaaatgg agccttaaaa aatttgaagt cataaacgct atagaacatt tgatgatgga
    17761 cagggatacc tggcagggca agaaatatga accaaacaat gatatcatga atatatttgt
    17821 ataaaataga tacgagacgc aagacacggg gataaaagca ataattctga ggcatactaa
    17881 acgtatatgc agacaccatg tatggtgtcc ctacgtaggg tcgccataca tggcgacaat
    17941 ctcgcgtcta aggtattgac aggagcatca aaacgtgcta acataattcg taaatattga
    18001 tatatgcaag gaacgggaat agtagaagaa tgaacagctt caagagagcc ggagacaggt
    18061 gggaaccggt agttttagtt cttggaactc gcccgggagc agcttcctga attgacagta
    18121 aggtaagccg tatttcctgc gttaaaagga tagacggtaa tgatgcgccg tcgaaaaagc
    18181 ggatttattg gtatggtatg ccgtaaatca aaaagagtgg taccgcgaat atacttcgtc
    18241 tcttattgag atgaagtttt tttattgtta aggaaggagt gttattaatg ccgtattcaa
    18301 aggatatcga caggaagtgg cagaaaagat gggaagaaac agggctctac aagtttaatc
    18361 cacaaaatgc agataagaag ctttattgtt tggagatgtt ctcatatccc tcgggggcaa
    18421 atcttcatgt agggcattgg tataattacg gtcctacgga ttcctggtcg cgtataaaga
    18481 ggatgcaggg ctatgaggtg tttcatccta tggggtttga tgcattcggc cttcctgcag
    18541 aaaactatgc cataaagacc ggaatacatc cctgggattc cacaaagaat aatattgcca
    18601 caatggaagc gcagctcaga gagatggggg caacctttga ctgggactat gaggtagtaa
    18661 cctgtgaccc ctcatattat aagtggaacc aatggttgtt cctgaagctg tacgaaaacg
    18721 gccttgcata caggaaggaa gcaccggtga actggtgccc caggtgcaac actgtcctcg
    18781 ccaatgagca ggtagtggaa ggccattgtg agcggtgtga aactgaagta atcaggagaa
    18841 acctgactca gtggttcttt aaaatcacgg cttatgccca ggagcttctg gataagctgg
    18901 atgaaatcga ctggccggaa aagactaaga aaatccagag aaactggatc ggaaggtcgg
    18961 aaggttcgga gatagagttc aaaatataca gaaaagacct tagcttcaag acgtttacca
    19021 ccaggatcga tacacttatg ggagttactt atgtggtgct tgccccggag caggagctgg
    19081 tggatgtaat tactgctcct gagcagaagg aagctgtaga agcttacaag gattttgcca
    19141 gaaaagcaag cgaaatagac aggctttcaa ctaccaggga aaagaccgga gtatttacag
    19201 gggcatatgc ggtgcacccc atatccggtg aaaagatacc tgtttggata tcggattatg
    19261 tccttgccac ctatggtacc ggctgtgtaa tgggagttcc cgcccatgat gaaagggact
    19321 tcgagtttgc tgaaaagttc ggattgccga ttcccagagt aattaaaggg gttggggatg
    19381 ttgatgacaa gttgcccttt gttgaatacg gagtgcttgt caacagcgga aaatttgacg
    19441 gtatgacatc ggaagatgca ttggtcaaaa tcaccgaagt tctggcaaag gaaggcaggg
    19501 gagagcttaa aataaattac aggctcaggg actggctggt atcgaggcag aggtattggg
    19561 gcactcccat accgataata cactgtgaag ggtgcggggt agtgcctgta ccggaagagg
    19621 atttgccggt aatgcttccc tatgatgtgg agtttactcc cgatggagaa tcgcctctta
    19681 aaaagagcaa ggagtttatg aataccgtat gtcccaaatg cggcaaaccc gcacagaggg
    19741 atcccgatac ccttgatact ttcgtatgct cctcctggta tcagttcaga tatccggata
    19801 ataagaacag taaagaacct tttaataagg agcggataaa taagttacta cctgtagaca
    19861 tatatgtagg aggggctgag cacgcagcaa tgcatctgct ctatgcaaga ttcattacaa
    19921 aagccctgag ggatatgggc tgcctgaatt ttgacgagcc cttcctgtca ttggtgcacc
    19981 agggagtcat actcggtgct gacggggaga agatgagcaa gtccaggggc aatgtcgttt
    20041 ctccggacaa atatataaat gaatccggat ccgacgtatt caggacctat ctttgcttcg
    20101 gcttcaacta tattgaaggg gggccctgga gcgatgacgg aataaaggcg attatcaggt
    20161 tcctgggaag agcggaaagg atggttgaga gagtatatga actcagaggg caaaaaagcc
    20221 gaaatgaagt ggctgagaaa gaaaaggaac ttaattatgt aaggcacatg gcaataaagg
    20281 gcgttacaaa ggattcggag atattccagt tcaacacctc catagccaag ataatggagc
    20341 ttttgaatgc attgtataag tatgatcagg aagttgaata caagaatata ccatactttg
    20401 aagaggtcct ggcggatctc ctgaagctgc tggctccctt tgcacctcat tttacggagg
    20461 aaatgtggga gaagctgggg tatgaatact cggttttcaa ccagaaatgg cctgtatggg
    20521 atgaaagcgc tctggtaagg gatagtgtag agctggctat acagataaac ggcaaggtca
    20581 agggcagaat agaagtaccc tctgctgctt cagaaaagga aattgaggat atggctatgg
    20641 caaatccgga gacaaaggga ttgattgccg gaaaacaagt gttaagggtg atagtagtaa
    20701 agggcaggct tgtgaatata gtcattaagt gattctgagg catacactac agcaatacaa
    20761 ggctgtctgt atgccttatt tgtttcaaag gcaaggtatt taccccttta atgcgggggt
    20821 cggcaaaaca attgagataa aaaatggaaa atatgaaatt catttgatta ttccagaaaa
    20881 ttgtaatatt atatgattaa taaagttgta ttgaagcaat tgatgtcgaa atttcagggg
    20941 aaagtcaaga aaagggggag ttatagatgg ccggcttttt atctggctat gtactggtta
    21001 atgttatact tggagcggcc ctgtttatgt tgcgcagcaa tgtattagct gttattatat
    21061 taatgctttt gattatatct tccggaatca ttttcatgac acggcttaaa gggatagcag
    21121 tcaggattgg agacaacatt gaagtaatca ataaaggtca gttgaacaaa aacgtcagga
    21181 agatgggaat tgatatgttt gacctcatat catcaaggat aaatgatttt ttattcaagg
    21241 tgagagccct cattgcttcc tttggtgtcg tatcaaaaaa ggtaatgaaa gatgcccatg
    21301 aggtggagaa acaagcggag gctatcaagt attcctccag tgagatagca actacgatac
    21361 aaagtgttgc tgagtcggta agcaagcagg ctgcatatac ccataacatg atggaaatgg
    21421 tgcagaactt cgcaaaagat gctgaggata taaatgcaaa tgcaaataca agttttaatg
    21481 ttgcgaagga aacaaaagat ataatagagg acagcttcag gaagttcgaa gacataagaa
    21541 ttaaaataca agaatccaag gaatacaaca agaaggtgct tcaggctctg gacagcctgg
    21601 atgaaaagct ccgggcaata gatacaataa ctgaagctgt tgaaggtatt gcatcccaga
    21661 cacagctgct ggcgctgaat gctgcaatag aagcagccag ggcaggagaa gcaggcaggg
    21721 gatttgcggt agtagcaggc gaagtaggga agcttgcgga cgactcttct cagtcagcaa
    21781 aagaaatcaa gcagttgata gacggaatta ccggtcagat aagcgagttg tcggtcaata
    21841 taaaggatga ggcggatgcc atagataaga atatggtata cgcaacggag gcattaagca
    21901 agtccgatat tataaacgat acacttgtaa gcaatatgca ggcggcaggg aagataacaa
    21961 cacttacaaa aacacagctt acaagtattg acagcattga aaagcagatt gaaatgatta
    22021 atgatttgac gcagcagaat gcggcagtat cggaagaaat aggagcatca acccaggaac
    22081 agctggctac cattgaaaca gtccatggaa atattctgca gttaaaagaa agactggaag
    22141 aatccgattc gattatatca aactttatga agggcttcca gcttaatgat tcaataaggg
    22201 agaaaattaa gaaaactcag gaattggtaa aggaaataac cagcagggga gatttgttta
    22261 gttctgataa gagttccgta caaaaatatc ttaatgagca gcaaagcaga tatgagtatg
    22321 ttgagtttat ggcattaatt aactccagcg gctattatga gttagcgact caggatgtcg
    22381 atgaaagcct taaggattgc agtgcaaagc cttacttcac aagggcgatc cagggggaag
    22441 aatatacatc ggaggagtat atatcggtca ttaccaataa ttataatata acagtgtcaa
    22501 tgcctgtcag gcagaatggg acaatcaagg gagtattaat ggcggatatt aatataaatg
    22561 agaactgatt aatacaagta atttgataaa ttacggaagt ccggtaacag acagcctgtt
    22621 ccggacttta tttcatgttg aatttcattt ttccgggttt cacttttcca tacagtttaa
    22681 taaattgaag taggggatta ttaagacctt acttcaattt atcgggaggg gaagaagtga
    22741 agccctacaa gacaaatcct atgatagcag aaaggatttg gggaggaagg agattatgca
    22801 gttacaataa aaatgtggga gataaaaacg caggggagag ttgggagacg gtatcaccgg
    22861 aaggagaaat tcctgtgctg ataaagctga tagacgccag ggaaacctta tcggtacagg
    22921 tgcatcccga tgatgaatat gcaataaggg ttgaaaatgc acggaatggc aagagtgaag
    22981 cttggatagt attggagtgt gaggaagatt cctttattat atatggattc aacaggatgg
    23041 ttcagaagaa agagcttagg gaactgctgg aaatggggct catttccgga gtacttaact
    23101 atataaacgt gaaaaaagga gattgtatat ttgtgcccgc aggtacggta cacagcatcg
    23161 gaagcggtat attggcatat gaagtgcagc agccttcaga cctgacatac aggatttatg
    23221 attggaacag aacagattcc cgcggaaaag gaagagagct gcatatagac aaggcgctgg
    23281 atgtaatcaa ttactcttcg ggattaccct gtgtgtttaa tgtttatgat tcattagagg
    23341 atggtattat taatataatt gaatgtgaat attttgcatt aagcttcaga tttatcaata
    23401 aggaggagtg ccacttttat gaaccgggca agttcagggc cattaccgca gtatcgggca
    23461 atttgctgct ccgatacgaa gattttcaat tgtcggtgaa aaagggggat acttgtataa
    23521 taccggagga atacgacagg gaagtcaggg ttgaggggct gggggctgcg gaatatatcg
    23581 tggctgccgg cagtaattaa gatgtaacac aattattagt gaatttatga tataataacc
    23641 attaaaatag tatgaaaaca atgtttaatt gaaaggggtg gcaatatgac agcgagtatc
    23701 agattggata acaatgaagc aaagcagact aaacagataa taaggttttt taagaaaggc
    23761 gtaaagatca gatacaataa gaagcttaca gggaaggtca ggctatatat cagtgaaaag
    23821 aattcagtga agaacattag tgatatattg tcactctatg aaaacagatt gaattaataa
    23881 agaagcatat gcagccatgt acagcatggc ctgcttttta ttatgccgat acttgttaaa
    23941 aaaagtgaaa tgcaattgat tatttaatta ttttgtaata ttatgagatt atggagtgtg
    24001 gagcattatg ggattactga gcctatacct tataattaac atagtttttg gtgcggcatt
    24061 gtatttaatg cgcagcagcc agttggccgc tgcagcatta ataatactaa tgacagcatc
    24121 aggatttgtt tttattgccc gattaagcag catttccgga aggataaagc ataacataga
    24181 agaaataagc aaagggcagc tgaacaaaaa cgtcaaaaaa accggtatca ttatgtttga
    24241 cagcatagcc tcggcgacga atgagttttt gctcaagatc agaggcttga tcggcagctt
    24301 tggggatgta tccaaaagga taatgaagga tgctcatggg gtagagaagc aggcagaggc
    24361 aataaggtat tcttcgggag aaatagcctc aacgatacag agtgtggcag agtcggtaag
    24421 caatcaggct gcatataccc ataacatgat ggaaatggtg caggacttca caaaaggggc
    24481 ggaggatatc agcgaaaatg cagagacaag ccttaaaatt gcaaaggaga caaaaggcac
    24541 aatagaggac agcttcggca agttttcgga tataaagttc aagatacagg aatccaagga
    24601 atacaacaaa aaggtgctgg aggctttgga caacctggat aataaaataa gggcgataga
    24661 tacaataaca gaagcagttg aaagcatagc agcccagaca cagctgctgg cgttgaatgc
    24721 agctatagag gctgccagag ccggtgaggc gggaagaggg tttgcggtgg ttgccggaga
    24781 agtagggaag cttgcagatg actcttcaca atcggcgaaa gagataaagc agctggtaga
    24841 cggcattaca agccagatag gggagctgtc ggtgaacata aaggacgagg catccgctat
    24901 agacaagaat cttaagtttg cggaagaggt actcagtaaa tctgacgtaa taaacagtac
    24961 tttgacggat aacatgaagg ctgccgagaa gataacgatg cttacaaagg agcagatgaa
    25021 gaatattgga agcatagaga aggagattca aaaggtcaac gatgtaacac agcagaatgc
    25081 ggcggtatcg gaggagattg gagcatctac tgaagaacag ctggctacca tagaagcggt
    25141 gcatggacat attgttatgc ttttggaaag gcttgaagaa tccaacgcaa taataggcaa
    25201 ttttatgaag ggtttcgttc ttaccgatga agtaaaggaa aaggttaagg aaactcagac
    25261 tgcggtcaat gaattggtaa agaacgaagg catattaaga atggataaga atgctgttga
    25321 aaaactcttt cgagagcatc agcagaaata taatttcata gaattcatgg gtctgataga
    25381 cagcaaggga tatcttacct tttctaccgt agaagtaccg gaaaaccata gagacgccag
    25441 tgcgaagcca tattttataa aagcttgcca gggagaggct tatgtatctg aagaatatat
    25501 atctgtggca acaaacaact ataatatttc cgtaaccgta ccctttcagc ggggcggtat
    25561 attcggggga cttattatag ctgatataaa tattaatgag aactgaaaat caaaacaatg
    25621 ggggagtgtg gagaatgaca ggctttctat tgggttatac agtaattaac cttattctcg
    25681 gaactgtact gtttatgtat cgcggtaacg aattagccgt tattttactg ataattgcga
    25741 tggcagcaac gggacttctg attttggcaa agctgaacag tatttcgtca aggatcggaa
    25801 acagcattga agaaataaac aaaggacagc tgaacaaaaa cgtcaaaaaa accggtatca
    25861 ttatgtttga cagtatagcc tcggcgatga atgagttttt gcttaagatc agaagtttga
    25921 ttggcagttt tgacgatgta tccaaaagga taatgaagga tgctcatgag gtagagaagc
    25981 aggcagaggc aataaggtat tcttcgggag aaatagcctc aacgatacag agtgtggcag
    26041 agtcggtaag caatcaggct gcatataccc ataacatgat ggaaatggtg caggacttca
    26101 caaaaggggc ggaggatatc agcgaaaatg cagagacaag ccttaaaatt gcaaaggaga
    26161 caaaaggcac aatagaggac agcttcggca agttttcgga tataaagttc aagatacagg
    26221 aatccaagga atacaacaaa aaggtgctgg aggctttgga caacctggat aataaaataa
    26281 gggcgataga tacaataaca gaagcagttg aaagcatagc agcccagaca cagctgctgg
    26341 cgttgaatgc agctatagag gctgccagag ccggtgaggc gggaagaggg tttgcggtgg
    26401 ttgccggaga agtagggaag cttgcagatg actcttcaca atcggcgaaa gagataaagc
    26461 agctggtaga cggcattaca agccagatag gggagctgtc ggtgaacata aaggacgagg
    26521 catccgctat agacaagaat cttaagtttg cggaagaggt actcagtaaa tctgacgtaa
    26581 taaacagtac tttgacggat aacatgaagg ctgccgagaa gataacgatg cttacaaagg
    26641 agcagatgaa gaatattgga agcatagaga aggagattca aaaggtcaac gatgtaacac
    26701 agcagaatgc ggcggtatcg gaggagattg gagcatctac tgaagaacag ctggctacca
    26761 tagaagcggt gcatggacat attgttatgc ttttggaaag gcttgaagaa tccaacgcaa
    26821 taataggcaa ttttatgaag ggtttcgttc ttaccgatgc aataaaagaa aagatcaata
    26881 agactcagga attattaagg gaaattatac aaaaagaaaa cataatggca ttggataaaa
    26941 attcagcaga gagatatatt aaagaacagc agaaaaaatt agaatacata gaacttatag
    27001 taattattga caacagggga tacattacgg gagcaacaac cgagctgcct gaatttgtca
    27061 gggattgcag ctcaaaaccg tattttacaa atgcgatgaa gggtaatacc tacgtatcaa
    27121 aggaatatat ctcatgcgct tccggcaact acaatatttc ggtttccatg cccataattg
    27181 tgaatgggga actcaagggt gtaataaatg cagatataaa tataaatgag aattaagctg
    27241 atgcctatcc taaaatgcat ggagatgtga ggataaacct tggcaggcag gtgaaaaaat
    27301 gcataaatat gttaaaagac tgaaacgcag ggcagaatca ggagacgagt cctctaagga
    27361 taaatatgaa gaaatcatgg aatgccttaa tacaatggat gatgacagag tggccaactg
    27421 tattattctt atgaagaagc atattcccga actctggaac cagagtgcac tgctccttaa
    27481 aaaatacata atcgaagagt ccagaagtct tgaatttttg agcaaggctg aagtagagct
    27541 tattgagttc ctgagggatt accttaggga tgaaggagat gacaggcatt aaaaaaaggc
    27601 gtattgcgcc ttttttgatt attcgccgtt atttctatac ttgtagtcgg aatttgttat
    27661 tttaaaatta ttctttaaac cggaggaaat ataaacccct ttgtcattgg ttatgaaaac
    27721 ggcttccaca ttttcaaggc tctcaaccag cttcattcct tcctccagtc ccataagaag
    27781 tacagtagtt gaaattccgt cgccatctat ggatttgccg gtaattattg atacgctgct
    27841 caggttattc tcaacgggat accctgtaaa ggggttcaat atatgatgat atatcttgcc
    27901 gttttcttcg aagtatcttt catatgttcc ggaggttact accgcctgat cctgaatctg
    27961 cactatccca agaaagtctc ctcttggatt tgagggagtc tggattccta ttctccaagg
    28021 gttgccattt ggattaccgc ctattgttat aacattgccg ccaagattaa tgattgcatg
    28081 ctctactccg ctttccttca atatgcgggc agcttcgtca gctgcatagc cttttgcaat
    28141 tgacccaaga tccacttgca tgcccggttc ctcaaggaag gccgtatggt tcccggggtt
    28201 caggctcagc ttactgtaat ccaccagtcc cgcagcttcc tccagcttgt ttttttcggg
    28261 gactgcagca tagtcagtac caatattcca gagcttaacc accgggccta ttgttatatc
    28321 aaataatccc ccggacttat gggaatactc cttgcctctt tctacaacat agtaggtatc
    28381 ggggctaagc ttgacttcat ttttgcccga agcttgatta agggatatga tttcgctgtt
    28441 ttctgcatta tttatagtca ttctgccctc aatttcctgt attctttcaa atgccttatc
    28501 cagtaatgct tcatcctttt tatcatatac cgttatctct acaattgttc cgagaaggaa
    28561 gccggttttg gacagaggct tggcagaagc acttggaaag ggtttctgac atccggacaa
    28621 aaggagcact gataataaag caagagccgt cagcctcagt tttgcatcaa gaatcaaaat
    28681 gggcacctcc aaaatatagt aaaaaaataa tcatattaaa aaatgcaaat aaatttacat
    28741 attcaattct atatgataca ttagtattat tgcaactata attttaataa tgataattaa
    28801 attcaatata tattattaat tataccatac tattactata gacattgtcg gttgtataat
    28861 atacaatatg ttttgcactg aacatgtaat ataagagagg gctttttatg aagataaaaa
    28921 agggagacct gataataatt gtaattcttg ttgctgcgtt aatttcatgg cttgtcataa
    28981 ataacccggg caagccggag gacgagagat tgattgttat agaaacaaac ggggatattt
    29041 atagggaaat acccgtagaa acgggaatgg agcagcagga aatccatata cagtttgaga
    29101 acggcaagta cattgacata atcgttgatg aaaacggcgc atatgtcaag gacgtgatat
    29161 gcccggacaa ggtatgccag aaaacaggat tggtaagcag agtgggacag agtatagtat
    29221 gcttgcccaa cagggtcgtt gtatatatag aaggaagagc agaatccgaa atcgacggta
    29281 tttcgttctg ataacaggcg gaggaatgaa tatgagcagg acaaaaaaaa tggtgctttt
    29341 ggggatattg gtctctcagg cattaatact taatttagtg gaaagactga taccggtgcc
    29401 tgtaccggtt ccgggaataa agctggggct ggcaaatgcc atttcactta ttgccataat
    29461 acttttcggg ccgaaggaag ccctggcagt tgtaggtatg agaacttttt taggttctgt
    29521 attcggagga gggatatctt cctttatata cagctttatc ggcggtacaa taagcaccat
    29581 ggccatggca atcatgtaca ggaaattcag gacactgttc agtctgcctt caataagtgt
    29641 cattggagca gtattccata acatcgggca aatactggtt gccagtctga taattgagaa
    29701 tgcaaagctt ttttattact tgcctgcgct gttgatttca gctgtgatta caggtttatt
    29761 tattggattt gcagttcaat ataccctgaa accaatgagc catgtacttg gaataggaag
    29821 tgatactaat gtggaatgat aacagtttct taaaaaagga aatggatgct gtagaagata
    29881 ctataaaaaa ttctttaaga acaagaaaca aaacagtaaa ggaagcattg gtcaggtttg
    29941 cagaaagcgg cggcaagagg ctgaggcctg cttttacaat acttggaggc acatttggcc
    30001 aatatgacag caatactata gtgcctattg cagctgctct ggagattatc catatggcaa
    30061 ctcttattca tgatgatata attgatgatg caaagctgag gcggggaaat gaaacggtgc
    30121 actcggtttt gggaaaggat gtggctgtat acagcggtga tttcttgctt accagggcct
    30181 tcatgctggt agcggattat gcggatatcg atatgctgaa acagattgca aaagcttcag
    30241 catatatatg tgacagtgaa atagcccaga atgagcagaa gtttgatacc aacgtcagcg
    30301 tcaaacagta tcttaaaagg atagggggaa aaactgcggc gctttttggt ataagtttgt
    30361 atatgggagc ttataaagcg ggctgtccca ggaaactggt gaatagactg ggcgttcttg
    30421 gcaataatat tggtatggct ttccagatag tcgatgatat tctggatttt accggcaatc
    30481 aggcaaaagt cggcaagccg cttctaagag atgctgctca ggggatatat actttgccgg
    30541 tactgtatgc gcttcattca gaatatagag aggaagcaat aaaggccatc aacaaaaccc
    30601 aagaggaaaa gggcgcaaat ttgtatgagg ttttggatgc ttccaaagca attgaaaggt
    30661 caaaaaatat tgcggaaaaa ttcataggca aagctatgaa gatagcggga gaactgccgg
    30721 aatccatagg cagggatata ataatggaaa taattgatga gcagcttgaa aggaagtttt
    30781 agcttagtac ttgtagcatc aaatgttgga tgcagctttt tgacgttgca aggggctata
    30841 atacgtatta atatatgttg tgaatggatg ctttgcactg caacgcattc attttatttt
    30901 acggcattga atcattgcgt aaaatatatc ataataatat atggatattt gtataggggg
    30961 tttaacgatg aacattattc aggcaatcat actggggctg gttcaggggc tgacggagtt
    31021 tctgcccata agcagttcag ggcatctggt attgtttcag aaaatatttg gtattaacga
    31081 accgacattg gtgtttgaca caatggttca tgtaggaacc ttggcagctg tgatgacggt
    31141 gctgcggaag gagatattat cccttctgaa aaggcctttt caaagactta cctggctgct
    31201 catcgcaggt acgataccaa caggaattat cggaatagct ttcaaggatt cctttgaagc
    31261 aatgtttcaa agcggcagca cactgggttt tgaatttctt gccaccggta ttattattct
    31321 ttttgccgga aggcttaaca acggaggaaa gcatatagag gagacaactt ttttggatgc
    31381 cgcatttata ggaattttgc agggggccgc aataatgcct gcaatttcaa gatcagggct
    31441 tactatttca ggtgccctta tgaggaatct ggacagggag ttcgcagcca ggttttcttt
    31501 ccttctgtcg atacctgcca tattgggagc tgccctattc caggcaaagg acattctgga
    31561 ggcgggaagc tccggaatca taaatgctcc aatcgttgca ggcaccattg ccgcggcagc
    31621 ggcaggctat ttttcggtaa gattcatgat tatgcttgta aagaagggca gcatgaagta
    31681 tttttcctat tatgtgttta taataggggc attggttata attgaccaat acataaccca
    31741 cctgttcttt tagt
//
LOCUS       DAOQUR010000026        30733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1736_length_30733_cov_11.555740, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000026 DAOQUR010000000
VERSION     DAOQUR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 30733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30733
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1736_length_30733_cov_11.555740"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..499)
                     /gene="ffs"
                     /locus_tag="PKI50_07070"
     ncRNA           complement(234..499)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI50_07070"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            691..1047
                     /locus_tag="PKI50_07075"
     CDS             691..1047
                     /locus_tag="PKI50_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03501.1"
                     /translation="MKKSLWIALALIIGILPGFFLVFNFMFSDIISLNERILSFLIVI
                     AAYLILGAAFGLAGSDIGWKWGIWISLPAVIIALAYSFSEAGTLAINLAYSASALGSS
                     AISAYLTSKFLRKRKQ"
     gene            complement(1078..1437)
                     /locus_tag="PKI50_07080"
     CDS             complement(1078..1437)
                     /locus_tag="PKI50_07080"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNT03502.1"
                     /translation="MGLWGKDIARTKYVGNVYVDQISSIKKENKALFRIASKCENCNN
                     NDYYSIYETFRLFRILSIPLVQCDTVYYFSCPECNFGFKLEDEEFKKLEKIALINSKY
                     MEGSITKSELETGLKDI"
     gene            1650..2570
                     /locus_tag="PKI50_07085"
     CDS             1650..2570
                     /locus_tag="PKI50_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT03503.1"
                     /translation="MKKYEAYLPYLAGFTVALIFGLSFMFTKQALETLPTALLLSSRF
                     SLAALLLTFLWLSGAIKINLKNKPLKKLMLLSLFQPIAYFIFETTGVKLTSSSEAGIM
                     IALIPVVVSIFAAVFLKERPSKSQLFFIAASVLGVIFIVLMSGSEDSTGHFAGILFLF
                     GAVLTAGVYNILSRKFSADFTPVEITFAMMWAGAAVFSIVSAVTGMINGNLDDYITYL
                     SSASTLIPILYLGILSSVCAFFMMNYMLSKMPASNSSVFSNLTTVVSIIAGVFIRHEA
                     FHWYQFAGSVVIILGVWGTNRCKPRHEIQL"
     gene            complement(2606..3775)
                     /locus_tag="PKI50_07090"
     CDS             complement(2606..3775)
                     /locus_tag="PKI50_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT03504.1"
                     /translation="MSEFFEENNENNEMNNTFYREVIERKPEKSSRKFIALVLALGLG
                     LGTIFGYGIDNFLPSPGSNANNVYAAAVDNTKIVYSGTVSPVVPIAEKVSPSIVAISL
                     KTRTRDFFGRIFEGQGTGSGIIIDKQGHIVTNNHVVEGADDIIVILHDGKELEATLIG
                     RDSQTDLAVIKVDSDNLTVAELGDSSALKVGELAVAIGSPMGTEYAGSVTAGIISGLN
                     RTVSIGDDSIKLIQTDAAINPGNSGGALVNSEGKVIGINTIKFAETRVEGMGFAIPIN
                     EAKPIIEDLINNKKVLRPFLGIQGITISKEEAEEYKLIQGVYVRLVIPGSGAEAAGVR
                     RGDIITKIDDKKVLTIEDLIAEIQKHKVGDTVKIEVYNQANEYKTLTVKLTASND"
     gene            complement(3861..5270)
                     /locus_tag="PKI50_07095"
     CDS             complement(3861..5270)
                     /locus_tag="PKI50_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT03505.1"
                     /translation="MKNSIQKRLFITYGVAIIIGFAILALSLLRIFDQYFIENRKELL
                     YEQGRKVAREAATVLYSGNPNSRSLINDLQVLDRFLDAHIWVINSDGIIVAVSGLNEE
                     RLLGRQVREEKIGNLAKGESIEERGNFDGMLGESSLTVGYPVFVNNRFSGGVLVHASL
                     LEIQENFREIYRLTLWVILISVAIAYAILYIQIRRISEPLKEISGAAKVIAGGEFHKR
                     LNINTGDEIEELGNSFNNMAKSLERIEENRRNLIANISHDLRSPMTSIRGFIEGMIDG
                     TIPEDKREHYLNIVLDESNRLIKMTNELLELGNMQQGSLEIKKEAFELNETIRRKLVA
                     YEKGITEKKLDVVFSMFEEKTFVLSDQVLLERVLSNLMDNAVKFTPEKGSIEIRTSTK
                     EDRIKVEITNTGKSISSEELNRVWERFHKGDTSRGLHKGGYGLGLAIVREIISQLGER
                     IWVSSGEGYVKFTLTIEKA"
     gene            complement(5270..5950)
                     /locus_tag="PKI50_07100"
     CDS             complement(5270..5950)
                     /locus_tag="PKI50_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT03506.1"
                     /translation="MKAKILVVDDDFNIADLIRLYLEKEMYEVKTVYSGRTALDVFRS
                     WTPDLVILDIMLPETDGYEVCRQIRKISGIPIIMLTARGETFDKVLGLELGADDYLVK
                     PFEPKELTARVKAVLRRTMGSDTIVEEVVYPQLVINKSDYSITFQGRKADIPPKELEL
                     FYFLASHPNQVFTREQLLGKLWGYDFMGDSRTVDVHVKRLREKFSDEAGRWEIKTVWG
                     VGYKFEVR"
     gene            6095..6388
                     /locus_tag="PKI50_07105"
     CDS             6095..6388
                     /locus_tag="PKI50_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HNT03507.1"
                     /translation="MYDELHRHNFEGRTGRKASHFHNYSGVTTKNPDFEGHVHYMSGY
                     TTEENEHIHYYSILTSPDFQVDGGHVHFFQCITTMDSGHYHLIYGYTSVYTEY"
     gene            6542..7033
                     /locus_tag="PKI50_07110"
     CDS             6542..7033
                     /locus_tag="PKI50_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNT03508.1"
                     /translation="MTENEKQVYEVLNQLKIPYTQYEHVPVYTIQQLDELNMDMKGKH
                     CKNLFVRNDKGDKHYLIIVSQEKRADLKDIAKQIGSSRLGFASEERLMKHLGLTPGAV
                     TPFGLLNDEEKKVTVLVDEDLKNSIHICFHPNVNTATVSLLFDDFIKFLQWKGNEYQF
                     IKI"
     gene            complement(7160..8056)
                     /gene="ftcD"
                     /locus_tag="PKI50_07115"
     CDS             complement(7160..8056)
                     /gene="ftcD"
                     /locus_tag="PKI50_07115"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNT03509.1"
                     /translation="MNRIVECVPNFSEGRDLGKIEKIVNPFRGKDNVKLLDYKRDEDH
                     NRVVVTVVGEPQAVKDSILEAMGIAIELIDMTKHRGQHPRMGAVDVVPFIPIKNVSME
                     EAVELAKEVAGEASEKYGLPIFLYEKAAADPQRENLANIRKGEFEGMAEKIKLPEWKP
                     DYGPAEIHPTAGAAVIGARMPLVAFNVNLNTNRMDIANKIARNVRFLNGGLRYCKAIG
                     IELKDRGMVQISMNMTDYTKTALYRAFELIRIEAARYGVTIAGSEIIGLLPMEALIDT
                     AVYYLGVEDFSMDQILENRLME"
     gene            complement(8190..9872)
                     /locus_tag="PKI50_07120"
     CDS             complement(8190..9872)
                     /locus_tag="PKI50_07120"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNT03510.1"
                     /translation="MEIKVPSDIEIAQNSQMKTVSQIAEQWGIEDDELELYGKYKAKI
                     NLKIFERLKDKPDGKLVLVTAITPTAAGEGKSTTTVGLGQALNKIGKKAIIALREPSL
                     GPVFGVKGGAAGGGYSQVVPMEDINLHFTGDMHAITAANNLLAAAIDNHINHGNTLNI
                     DPRQIVWKRVMDMNDRALREIVVGLGGKANGFVRQDGFMITVASEIMAILCLSNDLMD
                     LKERFGRMVVAYNYSGEPVTCSDLRVHGAMAILMKDAIKPNLVQTLENTPALIHGGPF
                     ANIAHGCNSIIATKLGLKLGEYLITEAGFGADLGAEKFFDIKCRYGGLKPSAVVIVAT
                     IRALKMHGGVAKAELGKENPEAVDKGFENLEKQVENIRKFGLPVLVAVNRFVTDTEAE
                     IELLVKKCKVYGVEVALNECWSKGGEGGTYMAETLVKLVEGSAPDFKYLYNVEAPIAE
                     KIETIAKEIYGGDGVVFTPKAQREMQKFADIGLDKLPVCMAKTQYSLSDDPTLLGVPK
                     GFKVSVKEVRVSAGAGFIVCLTGDIMIMPGLPKIPAAEKIDIDENGVITGLF"
     gene            complement(10011..10087)
                     /locus_tag="PKI50_07125"
     tRNA            complement(10011..10087)
                     /locus_tag="PKI50_07125"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10051..10053),aa:Val,seq:cac)
     gene            complement(10161..11330)
                     /locus_tag="PKI50_07130"
     CDS             complement(10161..11330)
                     /locus_tag="PKI50_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT03511.1"
                     /translation="MLSIIKLRLLGLRDDFAVIVLMTVMALGFTAIFGISFDDFKPTV
                     LVVDEDKSSYSEALINELKGDRGLYFEVSNMKDAAAEVEEGKAIAALVIPKGFDEGIK
                     SGERASLGVLKIKDDILIFTMQKVVSGIVLKMAEGERIADITADFIHTLKPEAGQAVK
                     VFAYRSVMEAWEYKRPLEVTSSVAGTGADSSYDNLKHYMIGFSLFFSMYTMVFGIGTI
                     LNDRQYKTWQRMLITPVSKASILGGNMVTAYFIGAIQIGILILGGKYLLGIDWGSSIS
                     GILTVAGSFVFAVTSMGLMLSGVVKTQTQLSAVTPVVLTSTSMLGGCMWSLDIVNNKV
                     LLFLAELTPQKWAIQGMESIVSKGMGFEAAVYPAIVLMIMGVLFFAVGVKIMRFE"
     gene            complement(11293..12678)
                     /locus_tag="PKI50_07135"
     CDS             complement(11293..12678)
                     /locus_tag="PKI50_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT03512.1"
                     /translation="MKVISIIKKDLKTILSDKKALAIIIVMPLLLMTILGSALRGAFT
                     TGDDQSIARINIAVVKQYDKDGDSRRFDKALRSGFLVKGIGEEAAAELRVTGEDVDPE
                     RIFLEDFLGSEDVAKIIAYRVEKEETAVEWLKSGEVSAVVLLPENFIYDMKINLLTPF
                     RNKTDIRVLTHPDRNVSGQIVRSIVEAYSDSMTSVIIGKNVLIEAAIRHNLMEDSYKG
                     MKEALDDITEAMEGIRVKIDAVEAEGRKPIKSSEYYAAAMAAMFILFAAGNGGRMLLE
                     EKENITYQRMIIAGTPKMGILAGKFFTVFLIALLQISIMIIFSRFVLKVQWGSAYPVV
                     LISLCAAFAIAGIGSMLAAATYRAGNYKMANIFETVILQTMALLGGSFFPIDILPSIM
                     QKLSFLSISGAVLKAYLKIMMGYGVQEIMSNIAALAAIGFLTAVLSVLILRENGGMRD
                     AQYNKAKTAGA"
     gene            complement(12690..13619)
                     /locus_tag="PKI50_07140"
     CDS             complement(12690..13619)
                     /locus_tag="PKI50_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT03513.1"
                     /translation="MLKVVNLHKSFGGTKAVDGISFEVDSGEVFGLLGPNGAGKSTTI
                     SIISTLILPTEGEVYYKGANILKEPNSVRRNLGVVPQDIALYPTLTGYENLRFWGNMY
                     GLKGAELKKRINEVSEIIGLNGRLKDRVDKYSGGMKRRLNIGAALLHKPELLIMDEPT
                     AGIDPQSRNHILDTVLELNRQGVTIIYTSHYMEEVEYLCSRICIMDQGRIIASGTQEE
                     LVDLVKGETRINLRLDRTDKDILQRLEAINGVAGAELVQDEIVVSGENVDMILADIIG
                     SIAEHGMRISSIDVSKPNLEAVFLHLTGKALRD"
     gene            complement(13639..14637)
                     /locus_tag="PKI50_07145"
     CDS             complement(13639..14637)
                     /locus_tag="PKI50_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03514.1"
                     /translation="MKKLMIAILALVLTAAFIGCSFDSLEGYRKAAEKTDQIKKGRTE
                     GEISMVMDFDTTGMTEEQIKELYYFKNMKGSFNVAYDDELEKGIFRYYLIFGGLGFDL
                     DVFVNGEEIFMKLPVVGKYTRLNEMEKHMDDKQGEGEPEVISEETQDAIKEKWLGLLK
                     REDVFRGKDMVLTTPDGEVKANEYTIRLNDEQIKSLAENCIDILSKDKKLEENYGKYI
                     RKNVEFLKDTSLEKLLSDIKENIKKYTVESFGYTAYVDIDGYIVNEKVGITLEVDNAE
                     PASVTKVSYELDIKNWDINKDQEFDFPALTDENTLEMENIEQNMPFKIEALSNIKD"
     gene            complement(14880..15527)
                     /locus_tag="PKI50_07150"
     CDS             complement(14880..15527)
                     /locus_tag="PKI50_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT03515.1"
                     /translation="MIKVLLVDDQDILVEGLKLILGREEDIQIIGTANNGGKAYEACR
                     GLRPDVVLMDIKMPEVSGVEATGMIKKDFPDIKIIVLTTFNDDEYIYDALKSGASGYM
                     LKDASPSEIAGAVRTVYNGGALIQSSVAVKVLDKFSELARGDRSKHKDSRAELLTDRE
                     KEICRLIAEGKNNREIADELYLSEGTVKNHITRVLIKLDLRDRTQLAVFTIKNDL"
     gene            complement(15524..16690)
                     /locus_tag="PKI50_07155"
     CDS             complement(15524..16690)
                     /locus_tag="PKI50_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNT03516.1"
                     /translation="MEKGRRLFSIIKAILFTLLFCTAIYFENAQRQRLYALVFIFALY
                     LAVGFGRGLIRRYDNLYFLSFILDIALVYTLEYNSRLLINYFFHSFYIAVLLESSLLL
                     GLRRGIMIGAATVVVSLIKYIYLIYYNFNLSNVSQLVFFLMVNVLILVVAGFAQHNKE
                     ERERKDILYRELLDAHKKLKQYTDEVNRLSVVDERNRIARDIHDTLGHNMTALIMQLQ
                     MAEHLLKEDPKKSEDLLVSSVKTAKDSMASIREVVETLRGTGTALAPVEFIRKLVNDF
                     SIKTGVEIKLEISGEDNIQDSAANIAVYHIVQEAMTNSVRHGKAERISIRLDYSNESI
                     TFCIVDNGIGAETLKEGYGLKGIRERVEAFNGKFETGSSDGFYLKGIVYLEGKK"
     gene            complement(16831..17070)
                     /locus_tag="PKI50_07160"
     CDS             complement(16831..17070)
                     /locus_tag="PKI50_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03517.1"
                     /translation="MDFGQNTVIYNACEIARKLFTVNEKDEKSNGRVVRKCFMCDISS
                     CFDMGNICCKECSIKNCRYKCNFADKENCEHKYIE"
     gene            17255..17614
                     /locus_tag="PKI50_07165"
     CDS             17255..17614
                     /locus_tag="PKI50_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT03518.1"
                     /translation="MEFGERLKDLREEKNLTRHLLASILNVSYSTVSKYETNIRFPDK
                     DTLVALADFFDVSLDYLLCRSDIKDTADKALSKCKQQFMVTSSGNIYNPYEGLSPEAV
                     EEIEKFKEFVQHKYGKK"
     gene            17665..18144
                     /locus_tag="PKI50_07170"
     CDS             17665..18144
                     /locus_tag="PKI50_07170"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HNT03519.1"
                     /translation="MHYCQKDKIYSTKFLRDYFHGDSLKVPYDIDYDKSIWIKEMCDK
                     CLTGILHCLDRESRLAYLFRDVAQLSCEEIAEIMEQNPAAIRKTVSRSRKKLRNFLNN
                     ECVLFNPSGSCKCRMKNLVEDIMLPREYQELKRFVSRVNLYKESQSILPQKNYWISN"
     gene            complement(18283..19116)
                     /locus_tag="PKI50_07175"
     CDS             complement(18283..19116)
                     /locus_tag="PKI50_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT03520.1"
                     /translation="MQITVLVDNNTIIDRYLLGEPGVSFFIRADGKNILFDAGYSDIF
                     ITNAGKLGISLYDVDYAVISHGHIDHTWGLEPMIRMYSEAMIEKIGFRKPVLIAHPQA
                     FESKYHDNEVIGSVISEKALGDFFDMNLKREPFWITDRLVFLGEIERTNSFENKKPIG
                     KRAVQGNEEPDFLRDDTALAYKSEKGLVIITGCSHSGICNIIEYAKKVCNDDRIADVI
                     GGLHLLSPEEEVLELTKKYIKQAGVKKLHACHCTDLKSKLALSEAAELEEVGVGLVLE
                     F"
     gene            complement(19214..19855)
                     /locus_tag="PKI50_07180"
     CDS             complement(19214..19855)
                     /locus_tag="PKI50_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNT03521.1"
                     /translation="MDKNVKFVVDERIKRTVENLEKNNISAYRVENEEEALKKVGELI
                     PEGSTIGIGGSMTLFEVGAIDYIRKGNYNVLDRYKEGLTPSEMKEILRKGFFADAYLT
                     SSNAVTENGELYNVDANGNRVASMLYGPDRVIVIVGINKLVKDIEEAVLRVEETAAPA
                     NNKRLNKTNPCTKTGHCMDCNSEGRICNEYTIIRRQGVKGRMHVIIVNKELGY"
     gene            complement(19877..20827)
                     /locus_tag="PKI50_07185"
     CDS             complement(19877..20827)
                     /locus_tag="PKI50_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT03522.1"
                     /translation="MKKNVLGNTGIEVTELCFGALPMGPLQKNLPDEESAEIVAYALE
                     SGITFVDTAQLYRTYLPIKSAIERTGIRPVIATKSTAASYEEMEKAIKEALDGMGIDY
                     IDIFHLHAAKVDTDVFEIRKGALQCLLDYKSRGIIKAVGISTHNVKVVEAAAARKDID
                     IVFPLLNKAGRGILEGTEEEMRGAISLCGQNGKGIYLMKVLGGGTLIDEYKDCVEYGR
                     RLDYCHSIAVGMVSREEVMYNVKYFNGEKDLDDIIKIKKKKSIKIAQTVCTSCGKCIE
                     VCHSGAPGFDHTGKAYIDQSKCIQCGYCIPSCRFFAIRMV"
     gene            complement(20887..20962)
                     /locus_tag="PKI50_07190"
     tRNA            complement(20887..20962)
                     /locus_tag="PKI50_07190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20927..20929),aa:Ala,seq:cgc)
     gene            complement(20979..22244)
                     /gene="glyA"
                     /locus_tag="PKI50_07195"
     CDS             complement(20979..22244)
                     /gene="glyA"
                     /locus_tag="PKI50_07195"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNT03523.1"
                     /translation="MLKRTRITDPEIYEIVADELRRQEHNIEMIASESTAPLEVMELS
                     GCIFTNKTLEGYPGARFQAGSHEADRLELLGIERAKKLYGAEHVNFQVYSGSTANYGV
                     YAAVLKPGDKVLSMRLDQGGHLTHGSPANFLSKVYEYDFYGVNPETEMIDYDELEKKA
                     EEIKPRLIIAGGSSYPRLIDYERISKIAKAAGAYFMVDMAHISGLVAAKVIPSPVPHA
                     DFVTSSTTKTFCGPRSGFILCKKEYAKLIDKGVFPGSLGSMHLTTMAAKAWSMKYAAS
                     AEFRSIMKQILVNAKTLAEELSRLGFRIVSGTTDNHIVMVDLRSKGISGKLFQDALDE
                     VGITVNKNQIPFDPASPLVTSGVRIGVTSITQRGLKEVEVKEIAEIMNQVAAAPEDEA
                     NLEACRERAEALIARFPLYPAGSFEDYNV"
     gene            22448..23305
                     /locus_tag="PKI50_07200"
     CDS             22448..23305
                     /locus_tag="PKI50_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT03524.1"
                     /translation="MRFEKLYADPIIEDPGFPPESMAVVINSGGKNLYGVLYTAAGMG
                     PHPSMILLHGFPGTERNLDMAHAFRRAGWNTLVFHYRGSWGSEGCFSFQNVLDDVKAA
                     IEFVKSEEPARKYRIDRDNIVLVGHSMGGFAALLTAADDMDIKAYAAIAPFDFGAIGE
                     NSEGDDKALRFLQGMFKECIVPLKGNTVEELINEAMANGNKWSFINNAEKLAKHKLLL
                     IAAKRDNLSIPELHYYPLVNKMLKYNTENFEYKMIDSDHSFQDKRILLTEIIEGWLEK
                     QVQQKSNIK"
     gene            23419..23706
                     /locus_tag="PKI50_07205"
     CDS             23419..23706
                     /locus_tag="PKI50_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03525.1"
                     /translation="MSFLKNLSEKVVSTAKVVGDKSQDMVEVSKLKLHISQIEGDIKK
                     LKFNMGELVYNAFASDTEFPAEEVTRIGEEIKAKYTEIEETKIKIQEVQAN"
     gene            complement(23790..24476)
                     /locus_tag="PKI50_07210"
     CDS             complement(23790..24476)
                     /locus_tag="PKI50_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT03526.1"
                     /translation="MLKAFALGITTGTSATTFSPQVLINREQCAAMLFRAIKAIHPNG
                     DYSVAGIPDFPDQKDMANYAVEAAKYMSKLAIVKGDAKGYFMPKATTTAQEAAGYGMA
                     TREAAILMSVRTYGQMDTIKAAPAAAVPIASASVVGTWILGTLSGGQFNAVTGKYEGG
                     ASGLGQMYTFKEDGGKTWGARETLTDTSAYFTAGTDDSGKYLLLGEEGSVPPLADKEN
                     ALKYKLDTAF"
     gene            complement(24449..24997)
                     /locus_tag="PKI50_07215"
     CDS             complement(24449..24997)
                     /locus_tag="PKI50_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03527.1"
                     /translation="MTFINGKLYNNSERWSMSSLFQEDEELKAFYEGGWDYLKSHSVT
                     FRARFAESFDYGETTVLSPWSREFTLSANTKADYNKLINHAPTILSAEAKLKPDGEPY
                     FQVKLDKVPGEVQDLNSMASGSMRTEIWMRRAGDKDFKQIHYEWATWEYLEVSAKALH
                     LLLKTPLQTRKTLRCLRPLLSA"
     gene            complement(25246..25743)
                     /locus_tag="PKI50_07220"
     CDS             complement(25246..25743)
                     /locus_tag="PKI50_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03528.1"
                     /translation="MKRILACLLVCSCLLAVILPLGGCGIKKKAEQAISEKIVEKALG
                     DEVDIKGDTVTVKGEDGEEITVGGGKWPDTDLAKKIPEFKKGKIATSAFQDNNIMVIM
                     DEVKADDFEAYLKEIKKTYTQDVYESRTDEMIIFGGNDGNQITVALNFMVKDGTMGIS
                     VQKDE"
     gene            complement(26012..>27535)
                     /locus_tag="PKI50_07225"
     CDS             complement(26012..>27535)
                     /locus_tag="PKI50_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT03529.1"
                     /translation="RLFGFLFVLVLTLLSFVFIILLLTGTFTAGLREAEGFYSNEFKR
                     ISSAVEKEYGHVSASAVSLSSSLSQSMERFLSEKGLSTADLPEAPEVLEELLDREFDR
                     LLFSLEKTGASGAFIVLDATVNKQAEAAEFSRAGLYLKNMEPNIISSAPPDVTILRGF
                     PDIARDRGYSLHAQWDMEFMIDSPSHFFLPFEKAKEKPRPLSRLYYWSGAVTLPKTSE
                     DAMLCIVPLIDSSGNIFGVCGYEVSAMLFKLKFIPDSSAYQRIFCMLAPIEDSVLRVG
                     SALFSGNYDAFGVSGDKKPLIIKNRGRLLYSYEQQNGLSLAGLHKEIRLYPEDSAFAE
                     EHFAVGILMPQEDLGRRLAWMNIRLIVLCALFLLAGTAASVFIGNKYMNPIMKGLNAI
                     GSQDIDSIARTNITEIDEIIGQIKARRTGKAPLPDDIFTDFIGRVKTLTPTEKMIFRY
                     YLEGKSNGEILELMYISANTLKTHNNHIYTKLNVSSRNELSLYISLMEKSGRLSELN"
     assembly_gap    27536..27635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27636..28557)
                     /locus_tag="PKI50_07230"
     CDS             complement(<27636..28557)
                     /locus_tag="PKI50_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT03530.1"
                     /translation="MMKVRALCLMLIFLILLCSVGCSTKTVLNPKSPVTLTMWHNYGG
                     QMKNLMDEMIDEFNRTVGTEKGIIISVTSISGSSALHDKLISAAFGDPGAPELPDLAV
                     VYPKTALKLVEKDLLVDLSGRFTEEELSAYVPRFVEEGRLKGGLFVFPIAKSTEALFV
                     NKSIFDRFAADTGSTYDELSTFEGIMRTADKYHEWSEGKAFYMPDSVFNMAIIGMNQL
                     GEDFVQNNGLNTAGRVYKKIWDTYFLSAVKGSTAIFDGYSTDLFKTGDIVCSTGSTAG
                     VLFYPSTVTYADNTKENAEYAILPYPVFEGG"
     gene            29073..29360
                     /gene="gatC"
                     /locus_tag="PKI50_07235"
     CDS             29073..29360
                     /gene="gatC"
                     /locus_tag="PKI50_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNT03531.1"
                     /translation="MKISVEDVRYIAKLAKLSITFEEAQKMVGEFEGILTYFESIDRL
                     DSGDALPDPPEREMATVLRKDEISVFEDKEKLFLNAKSMRDSYIRVPKIIE"
     gene            29373..>30733
                     /gene="gatA"
                     /locus_tag="PKI50_07240"
     CDS             29373..>30733
                     /gene="gatA"
                     /locus_tag="PKI50_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNT03532.1"
                     /translation="MNIYEMTALEIRKGITEKQFSAIDAVTAFFERICEKEPLVDGFL
                     TLCREEALAKARQIDRKIKAGEPLGKLAGIPVAIKDNICTDDIRTTCASGMLQDFIPP
                     HDAAVVKKLKEADAVIIGKTNMDEFAMGSSTENSAFKKTRNPWDRERVPGGSSGGSAA
                     VVSAGMAALALGSDTGGSIRQPAAFCGVVGLKPTYGLVSRFGLIAFGSSLDQAGPMAN
                     NIKDCALALEAIQGEDKRDSTSVRHSVTEDYMSTIEKGVKGMKIGVPAEFFRSGLDEE
                     IAFYIKACMQKFKEMGASVEDISLPITEEGLSAYYIISSAEASSNLGRFDGLRYGYRT
                     ENFNGLDQLIEKSRSEGFGEEVKRRIMLGTFVLSSGYYDAYYNRAQKLRARIREQFIK
                     AFESYDLLISPASPVLPFKIGEKAGNPLEMYLSDIYTVNINLAGLPALSMPCGISRDG
                     LPVG"
BASE COUNT         8089 a         7607 c         5778 g         9159 t
ORIGIN      
        1 atagatacta ttaaaaaata cagtagcgtt ctaagcctaa aatttattct gttcatgaca
       61 aaaaatcccc cctctgtaca taattcggca tcatttgtta gtattattat acctgtaaag
      121 gaattaatat caaaatagtc ttcataaata agtcctaagg cttggttcta gttctgaagg
      181 cctatatata atgtaaaata aaaaacttgt ttgcacaagt ttttagtata tggccgtgca
      241 ccttccctcg acgtgcacct acaagcgtta cccgggcagt tagctccaat caggcagccc
      301 tgcggcacat gaaagggttc acttaccgct gcttccttcc ggacctgacg gggttcatga
      361 atttccattg cgcaggaccc aatcatcaac accacttacc aggggcagtc cttacaggcg
      421 aacgcctcag caaaggaatt caaccctgct gttgcggatt gcaggttaca gggcaccgca
      481 gtctccccat ctagcacggc caagattgtt aactttttgt gattaatata ttttgatata
      541 tttagctatt taattataag ctttcggata tttgatgtca atgcgtcaaa ataggctttt
      601 attgaagcat tttacaattg atgattggta ttataatatg aatacagaca acactattat
      661 catagatact tttgcaaagg agctgctatt ttgaaaaaaa gcctatggat tgctctcgca
      721 cttattatcg gaattctgcc ggggtttttt ctggttttta attttatgtt ttctgatata
      781 atatccctca atgaaagaat cctctctttc ctgatagtaa ttgcagctta tctgatcctg
      841 ggggcggcct tcgggctggc cggaagtgat ataggctgga aatggggaat atggatcagc
      901 cttcctgctg ttattattgc attggcctat tccttcagcg aggcaggcac gctggcgata
      961 aacctcgctt attcggcaag tgcgcttggt tcctccgcta taagcgctta cttaacctca
     1021 aagtttttaa gaaaacgaaa gcagtgacgg attgcgtcac tgcttttgac cctgtttcta
     1081 aatatctttc agtcctgttt ctaattcgga ttttgttatt gagccttcca tatactttga
     1141 attaatcaat gctatctttt caagcttctt gaactcctca tcctcaagct taaacccgaa
     1201 gttgcattca ggacagctga agtaataaac ggtatcacac tgcaccaagg gtatgctcag
     1261 aatcctgaaa agcctgaagg tctcgtaaat actgtaataa tcgttattat tacagttttc
     1321 acacttggac gctattctga acaatgcttt gttttctttt ttaatactgc taatctggtc
     1381 aacataaaca tttcctacgt attttgtcct tgctatatcc ttcccccaaa gccccacagc
     1441 atatcacccc tcgcaatatc caaccggaaa ctatttatgt attgtatatt ctatcaataa
     1501 tttcaaaatc cttcccgcaa atcctataat tgtaatgaac cgtgctttcc aaactttaaa
     1561 tagacaagcc cctttcattt ctgctagaat aactatattc gggacatctt catcaaatgt
     1621 ctgcctcaaa atatatagga tgtgataacg tgaaaaaata tgaagcttat ttgccttatt
     1681 tggcaggatt tacagtagca ttaatttttg gattgtcctt tatgtttaca aaacaagccc
     1741 tggaaactct gcctacagct cttcttcttt cctcccgttt ctctttggct gcgttactgc
     1801 tgacctttct atggctgtca ggcgccatta agataaacct taaaaacaag cccttaaaga
     1861 agcttatgct gttatctctg ttccagccga tcgcatattt tatttttgaa acaaccggtg
     1921 taaagcttac atcctcatcg gaagcaggaa ttatgattgc tctgataccc gtagttgtat
     1981 caatttttgc tgcagtattc ctcaaggaaa ggccgagcaa atcccagctg ttttttatag
     2041 ccgcttccgt cttaggtgtg atatttatag ttttgatgtc cggcagcgaa gacagcacag
     2101 gtcattttgc cggaatactc tttctcttcg gagcagtact gaccgccgga gtatataata
     2161 ttctttcacg aaagttttct gccgacttta ctcctgtaga aattaccttt gcaatgatgt
     2221 gggcaggggc agcagtattc agtattgtat cagcggtaac aggaatgatt aacggcaatc
     2281 ttgatgatta tattacttac ttaagctcgg caagcacgtt gattcccatt ctttatctgg
     2341 gaattctctc ttctgtgtgc gcttttttta tgatgaacta tatgctttcg aagatgcctg
     2401 cttcaaactc ttcggtattc tctaatctta ccactgtagt atcaataatt gccggcgttt
     2461 ttatcaggca tgaggccttt cattggtatc agtttgcagg aagtgttgtt attatcctcg
     2521 gggtctgggg cacaaaccgc tgcaagcccc ggcatgaaat acagctttaa aacaaataag
     2581 agacagttgc ctgtctctta cttatctaat cattgctggc agtcagctta acagtaagcg
     2641 tcttgtattc atttgcctga ttgtacactt ctatttttac tgtgtctccc accttatgct
     2701 tttgtatctc tgctatcaga tcttcaattg tgagaacctt cttatcatct atttttgtta
     2761 tgatatcgcc tcttctgact ccggccgctt ctgcaccgct gcccggtatt actagcctta
     2821 cataaacacc ttgaatcaac ttgtattctt cagcctcttc cttgcttatg gttattccct
     2881 gtattccaag aaatggcctt aataccttct tgttgtttat caggtcctca ataataggct
     2941 ttgcctcgtt tatcggaatg gcaaaaccca tcccctcaac tcttgtttct gcaaacttta
     3001 ttgtgttaat ccctatgacc tttccttcgc tgtttaccaa tgcccctccg ctgtttccgg
     3061 gatttattgc ggcatcagtc tggataagct ttatggaatc atctcctatg ctgaccgtcc
     3121 tgttgagtcc gcttattatt cccgctgtaa ccgagccggc atattcggtg cccatcgggc
     3181 tgcctatggc aactgccaat tctcccacct taagtgctga agagtctcca agctccgcaa
     3241 ccgtcagatt atccgagtca acttttatta ctgccaggtc tgtctgggaa tcgcgcccta
     3301 tcagagttgc ttcaagctct ttgccgtcat ggagtattac tattatatcg tcagcccctt
     3361 caactacatg gttgttggtt actatatggc cctgcttgtc gattatgatt cccgagcctg
     3421 ttccctggcc ttcaaaaatc ctgccgaaaa agtctctggt tctcgtctta aggctgatag
     3481 caactattga cggtgaaact ttttccgcaa tgggcactac aggcgatacc gtcccgctgt
     3541 atacaatttt ggtattatcc acggctgctg catatacatt gtttgcatta cttccgggac
     3601 taggcagaaa attgtctatc ccgtatccga aaatcgtgcc gagcccaagc cctaaagcaa
     3661 gcaccaaagc tatgaatttt ctggagcttt tctccggctt cctttcaata acttccctgt
     3721 aaaaggtatt gttcatttcg ttgttttcat tattttcttc aaaaaattcg ctcatatctt
     3781 atccctccta acggatgtac ttgaactata ctataaatat aatccaaagt tttgaacaaa
     3841 atatgaacaa attgtaaact ttaagctttt tcaatagtta atgtaaattt tacataacct
     3901 tcgccgctgg agacccatat cctctcaccc aactggctga ttatttccct gacgatggca
     3961 agccctaatc cgtatcctcc tttgtgaagg cctcttgaag tatccccttt gtggaacctt
     4021 tcccatactc tgttaagctc ctcagagctt atcgattttc cggtattggt aatctccact
     4081 tttatcctgt cttcctttgt tgatgtcctt atctcaatgc tgcccttttc cggtgtgaat
     4141 tttactgcat tgtccataag attggaaagc accctttcaa gcagtacctg atcagacaat
     4201 acgaaggttt tctcttcaaa catgctgaaa actacatcaa gttttttctc tgttatgcct
     4261 ttttcatatg caacaagctt ccttcttatt gtctcattaa gctcaaaggc ttccttcttg
     4321 atttcaaggc ttccttgctg catattgcca agctccagca gttcattggt catctttatc
     4381 aatctgttgc tctcatccag aactatgttc agataatgct cccttttatc ctcaggtata
     4441 gtaccatcaa tcattccttc aatgaaaccc ctgattgaag tcatcggcga gcgcaggtca
     4501 tgggatatgt tcgcaatcaa attccgcctg ttctcttcta ttctttccaa tgacttcgcc
     4561 atattgttga agctgttgcc aagttcttca atttcatctc ccgtattaat attcaagcgc
     4621 ttgtggaact cccctccggc aattaccttt gcagctccgc ttatttcctt taatggttca
     4681 gaaatcctcc gtatctggat atacaggatt gcataagcaa tggcaacgga aatcagaata
     4741 acccaaagcg tcagtctgta tatttctctg aaattttcct gtatctcaag aagtgaagcg
     4801 tgtaccagca cccctccgga aaacctgttg ttgacaaata cgggatagcc tacggtcaaa
     4861 gaagattcac caagcatccc gtcaaaatta cctctctcct caatactttc cccttttgca
     4921 aggtttccga tcttctcttc cctaacctgc ctgcccagca gcctttcttc gttgagcccg
     4981 gaaacggcaa ctattattcc atcactgttt ataacccaga tatgagcatc caaaaacctg
     5041 tccaggacct gcaaatcatt aataagactc ctggaattgg gatttcccga ataaagcact
     5101 gttgcagctt ccctggccac cttcctgccc tgctcataca gaagctcttt cctgttttcg
     5161 atgaaatact ggtcgaatat cctgagaagt gacaaggcca gtatagcaaa gcctattatt
     5221 attgctacgc cataggttat gaacaatctc ttctgtatgc tgttcttcat catctcacct
     5281 caaatttata gcccacgccc caaacagtct ttatttccca tctgcccgct tcatcactga
     5341 acttctccct gagccttttt acatgaacat ccacagtcct cgagtcaccc atgaaatcgt
     5401 atccccatag ttttcccagc agctgttccc tcgtaaaaac ctgatttgga tggcttgcaa
     5461 ggaaatagaa cagctccagt tccttcggtg gtatgtccgc cttcctgcct tggaatgtaa
     5521 tgctgtaatc gcttttgtta attaccagct gaggatatac aacttcttca actattgtat
     5581 cagagcccat ggttcttctg agcactgctt ttactcttgc agtaagctcc ttcggttcaa
     5641 aaggctttac aagataatca tccgctccca gttccaggcc cagtaccttg tcaaaggttt
     5701 ctccccttgc ggtaagcata ataatgggaa taccgcttat cttccttatt tgcctgcata
     5761 cctcatagcc gtctgtttcc ggaagcataa tatcaagaat gacaagatcc ggagtccatg
     5821 aacggaatac atcaagagca gtcctgccgg agtataccgt ctttacctcg tacatctcct
     5881 tctccaggta cagccttata aggtccgcta tattgaagtc gtcatcaaca accagtattt
     5941 tggctttcat atcttttacc accgcctatt gaacatcttt ttgcctcgtt ttatccatta
     6001 tgatacatca atgtctgtga taattcaaat gcatagaaaa atagatgtca aaagcccttg
     6061 ccgctgcata gatttatagc agggggtatg caatatgtat gatgagctgc acaggcacaa
     6121 ttttgagggc aggaccggcc ggaaagcctc gcatttccat aattactccg gagtgacaac
     6181 caaaaatccc gacttcgaag gccatgtaca ctacatgtcc ggttatacaa ccgaggaaaa
     6241 tgaacatatt cactactatt caatattaac cagtccggat tttcaggtgg atggcggaca
     6301 tgtacatttc tttcaatgca taacaactat ggacagcggc cattatcatc ttatatacgg
     6361 ttataccagt gtttatacgg aatattagaa aattgcacgg caattttcag acacgggatt
     6421 cgagacgcga gacgcgaaat ttagggttag gcttctaagt taaggattac gaacactttt
     6481 atgcctcttg cttttgtggt aatataatta tacacagaaa tcaaggaggc atacaactgc
     6541 aatgacagaa aatgaaaaac aagtatacga agtgctgaat caactgaaaa ttccatatac
     6601 acagtatgaa cacgtaccgg tatatacaat tcaacagctt gatgaattaa atatggatat
     6661 gaaaggtaag cactgcaaga atctcttcgt cagaaatgac aagggcgaca agcattacct
     6721 tattattgtc tcccaggaaa agagggcaga ccttaaagac attgccaaac agattggcag
     6781 ctcccgctta ggctttgctt cggaagaaag actgatgaag caccttgggc tgactcccgg
     6841 tgcagtcact cccttcggcc tgctgaatga tgaggaaaaa aaggtaactg tacttgtgga
     6901 tgaagacctt aagaattcaa tacatatatg cttccatccc aatgtaaata cagcaactgt
     6961 atcgctgctt tttgacgact tcataaagtt cttgcagtgg aaaggcaacg aataccaatt
     7021 cataaaaatt tgaggcatgg ggacggaggg acacggggac atggggacat ggggacgttt
     7081 cgcgtgtccc actggaagtg ggacagggga aacgtcccca tgtccccatg tccccatgtc
     7141 ctcgcgccgc attattcttc tactccatta acctgttttc cagtatctga tccatggaga
     7201 aatcctccac gcccaggtaa tataccgccg tatcaatcaa ggcttccata ggcagaagtc
     7261 ctattatttc actgccggca attgtaacgc cgtatctggc cgcttctatt ctgataagct
     7321 caaaggctct gtaaagagcg gtctttgtat aatcagtcat attcatggaa atctgcacca
     7381 ttcccctgtc cttaagctca atgcctatag ccttgcagta ccgcaaaccg ccgtttaaaa
     7441 acctgacatt ccttgcaatt ttattggcaa tatccattct gttagtattc aaatttacat
     7501 taaaggctac caggggcatc cttgcgccga taaccgcggc tcctgctgta ggatgaattt
     7561 ctgccgggcc gtaatccggt ttccactcag gcaactttat cttctccgcc ataccttcga
     7621 attctccctt gcgaatattc gccaggtttt ctctttgggg atctgctgca gccttttcat
     7681 acagaaaaat cggaagccca tacttctccg atgcctctcc tgccacttcc tttgcaagtt
     7741 caacggcctc ttccatgctt acattcttta tgggtatgaa gggtactacg tctactgccc
     7801 ccattctggg gtgttgtccc ctatgcttgg tcatatctat aagctctatt gcgataccca
     7861 tagcttccag tatagaatcc ttcactgcct gaggttcacc cacaacagtt acgaccactc
     7921 tgttgtgatc ttcgtctctc ttataatcca gaagctttac gttgtccttt cccctaaagg
     7981 gattcactat tttctctatt ttgcccaaat ctcttccttc actgaaattc gggacacatt
     8041 caactattct gttcatcccg ctcattgctt tccccccttt atggtctagt gtctgaactt
     8101 ttatgtttca cataaaagtt ttacacttat atgactaaat ccctatattt ccagcattta
     8161 atttgctgca aatattacgg gtttcgtcac taaaataaac ctgttattac gccattttca
     8221 tctatgtcaa tcttttctgc tgcaggtatc tttggaagtc ccggcattat cataatgtcc
     8281 cccgtaagac atactataaa gcccgctccg gcagataccc tcacttcttt aacactaacc
     8341 ttgaagccct ttggaacacc taaaagagtg gggtcatcag acagcgagta ttgagtcttt
     8401 gccatacaga caggcagctt atccagtccg atatccgcaa acttctgcat ctccctttgt
     8461 gctttgggag tgaaaacaac accgtccccg ccatagattt cctttgcaat agtttcgatt
     8521 ttttcggcta taggagcttc aacattgtaa agatatttaa aatcaggtgc tgagccttca
     8581 accagtttta caagggtttc ggccatatat gttccgcctt ctccgccttt tgaccagcac
     8641 tcgttcaatg caacctctac gccatatacc ttgcactttt tgacaagcag ttcgatttcg
     8701 gcttcggtat cggttacaaa cctgttaacg gcaaccaata ccggaagtcc gaacttgcgg
     8761 atgttttcca cctgcttttc aaggttttca aatcctttat ccactgcttc cgggttttct
     8821 ttccccagtt ccgcctttgc aacccctccg tgcatcttaa gagcacgtat ggttgctact
     8881 attacaactg cggaaggctt taagccccca taacggcatt tgatgtcaaa gaacttctct
     8941 gctccaagat cagccccgaa gcctgcctcg gtaataaggt attcccccag cttcaagccc
     9001 agctttgtag caattatgct gttgcagccg tgggctatgt tggcaaaggg tccgccgtga
     9061 ataagtgcag gtgtgttttc caaagtctga accaagttag gctttatggc gtccttcata
     9121 agaatggcca tagccccatg aacccttaaa tcggaacagg taaccggttc tcccgaataa
     9181 ttatatgcaa caaccatccg gccgaatctt tcttttaaat ccatcaaatc attggaaagg
     9241 cacagtatag ccattatttc agaagccaca gttatcataa aaccgtcctg ccttacaaag
     9301 ccgttggcct ttcctccaag gcccaccact atttcacgga gtgccctgtc gttcatatcc
     9361 attactcttt tccagactat ctgccttgga tctatattca gtgtgtttcc gtggtttata
     9421 tgattgtcaa tggctgcagc aagcaggttg ttggctgcag taattgcatg catgtctccg
     9481 gtaaaatgaa gattgatatc ctccataggc actacctggg agtatcctcc ccctgcggct
     9541 ccgcccttca cgccgaaaac cgggcccaaa gacggttctc ttaaagctat tatcgccttt
     9601 ttcccaattt tattaagagc ctggcccaag cctaccgtgg ttgtcgactt gccttctcct
     9661 gcagcagtgg gagtgatagc agtaaccaat accagttttc cgtcaggttt gtcttttaat
     9721 cgctcaaaaa tttttagatt gatttttgcc ttatacttgc cgtatagctc cagttcatca
     9781 tcttctattc cccattgctc cgcaatctgc gatacggtct tcatctgtga gttctgcgct
     9841 atttcaatat cgctgggtac tttgatttcc atttaatcat tcctttcgta aaataatgtc
     9901 agaattatgt aaatgcatct aagagagaat gcaaatccat ccaaaaaggg tatccaaaca
     9961 cctctataaa aatagagcga tctttcgata gctctatgat tttttagcaa tggtggggga
    10021 tacgggattc gaacctgcga cttccacctt gtgacgatgg cactctaacc ggctgagtta
    10081 atcccccact cgaaaatcaa tatatatatt ataactctac atgttaacat tatccatagt
    10141 attttttatt atttgctaat ctattcaaac ctcataattt tcactccgac tgcaaagaac
    10201 aacactccca ttatcataag cacaattgca ggatacactg ccgcttcaaa gcccatcccc
    10261 ttggatacta tactttccat accctgtatt gcccatttct gaggtgtgag ttccgccagg
    10321 aaaagcagca ctttattgtt tactatatcc aaagaccaca tgcagcctcc cagcattgaa
    10381 gtactggtaa gcaccaccgg ggtaaccgca gaaagctgag tttgggtttt taccacaccg
    10441 gacagcataa gtcccatgga agtaacggca aatacaaaag aacccgccac ggtgagaatt
    10501 cctgaaatac tgcttcccca gtcaattccc agcagatatt tccctccaag tatcaatatt
    10561 cctatttgaa tggcaccgat gaaatatgct gtcaccatgt ttcccccaag tattgaggct
    10621 ttggatacgg gggttatcag cattcgctgc catgtcttat actgtctgtc atttaatatt
    10681 gttcctatgc cgaacaccat tgtatacatc gagaaaaaca aggaaaagcc tatcatgtaa
    10741 tgtttaagat tgtcgtagct gctgtctgct cctgtaccgg caacagaaga ggttacttcc
    10801 aaaggccttt tgtattccca tgcctccata acgcttcgat atgcgaatac cttcacagcc
    10861 tggccggctt cgggcttaag ggtgtgtata aaatccgcag tgatatctgc tattctttca
    10921 ccctccgcca ttttaaggac tatacccgat actaccttct gcattgtgaa tatgagaatg
    10981 tcgtccttga tctttaatac ccccaaagaa gctctttcgc cgctttttat gccctcatca
    11041 aagccctttg gtataaccag tgcggcaatt gccttgcctt cttccacttc cgccgccgca
    11101 tccttcatat tcgagacttc aaagtataat cccctatcgc ccttcagttc attgatcaag
    11161 gcttcggaat aactgctttt atcctcatcc acaaccagta ccgtcggctt gaagtcatca
    11221 aaagagatgc cgaaaatggc agtgaagcca agggccatga cggtcatcag aacaatcacc
    11281 gcaaaatcgt ccctaagccc cagcagtctt agctttatta tactgagcat ccctcatccc
    11341 tccgttctcc ctgagtatca aaaccgaaag cactgcagtt aaaaacccta ttgccgccaa
    11401 tgctgcaata ttgctcataa tctcctgtac cccatatccc atcataatct taagatatgc
    11461 tttcaaaact gccccgctga tggataaaaa gctcagcttc tgcattattg aaggcagtat
    11521 gtctatcggg aagaagctgc ctcccaaaag tgccatagtt tgcaagataa ctgtttcaaa
    11581 tatgttcgcc attttgtaat tgcctgccct gtatgtggct gctgcaagca tgcttccgat
    11641 tcccgctatg gcaaaagcag cacataagct gattaaaaca accggatacg cgcttcccca
    11701 ctggaccttc agcacaaagc gtgagaatat gatcattata cttatctgaa gcagggcaat
    11761 aaggaaaaca gtaaagaatt ttcctgccag tattcccatt tttggtgttc ctgctattat
    11821 catcctctgg taagttatat tctccttctc ctccagaagc attctccctc cgttaccggc
    11881 tgcaaaaagt atgaacattg ccgccattgc tgcagcatag tattctgagc ttttaatagg
    11941 cttccttccc tctgcttcca cagcatcaat ttttaccctt attccctcca ttgcttcagt
    12001 gatgtcgtcc aaagcctcct tcattccctt atagctgtct tccataagat tatgccttat
    12061 agccgcttcg atcagaacat ttttccctat tataaccgaa gtcattgaat cggaataagc
    12121 ttccacaata ctccgtacta tttgaccgct aacgttcctg tccggatgtg tcagcaccct
    12181 tatatcagtt ttgtttctga agggtgtcag aagatttatc ttcatatcgt atatgaaatt
    12241 ctcgggaaga agcaccaccg cagaaacctc cccgctttta agccattcaa cggctgtttc
    12301 ctccttctct actctgtatg ctataatttt tgccacatcc tcactgccca ggaaatcctc
    12361 aagaaatatc cgctccgggt ctacgtcctc accggtcact ctcagctccg cagcagcctc
    12421 ctctcctatt ccctttacaa gaaagccgct tcttaaagcc ttgtcaaacc tccgggaatc
    12481 cccatcctta tcatactgct tcaccaccgc aatatttatc ctggcaatac tctggtcatc
    12541 tcctgttgta aatgcccctc ttaaagcaga gcccaatatc gtcataagca ggagaggcat
    12601 cacaattatg attgccaaag cctttttgtc gcttagtatc gtttttaaat ccttctttat
    12661 gatacttatt actttcatgt tttatctcct taatccctca gggcctttcc tgtcaagtgc
    12721 aggaagacgg cttccagatt cggcttgctg acgtcaatgg atgaaatgcg cattccatgc
    12781 tcagctatgc tgccgataat atctgccaat atcatatcaa cattttcccc tgaaacgaca
    12841 atttcatcct gcaccaactc tgccccggct accccattta tggcttcaag cctttgaagt
    12901 atatccttgt ctgtcctgtc aagcctgaga tttattctgg tttctccttt tacaaggtcc
    12961 actagttctt cctgggttcc ggaggctatt atccttccct gatccattat gcagatacgg
    13021 ctgcacagat actccacctc ttccatgtag tggctggtgt atatgattgt aaccccctgc
    13081 ctgttaagct ccaggacggt atctaatata tgattcctgg actggggatc tatgcctgcc
    13141 gtcggttcat ccataataag cagttccggc ttgtgcagca gtgccgctcc gatattcagc
    13201 cttcttttca ttcctcccga atacttatcc accctatcct tcaggcggcc gttaaggccg
    13261 attatttcag aaacctcatt gatccttttt ttcagctctg ctcctttaag cccgtacatg
    13321 ttcccccaaa aacgcagatt ctcatagccc gttagtgtag gatacaacgc tatatcctgg
    13381 ggtaccacac ccagattcct tctgacggaa ttcggctcct ttaatatgtt tgccccctta
    13441 taatatacct ccccctctgt aggaagtatt aatgtagata taatggagat ggtagtggac
    13501 tttcctgccc cgttggggcc aagcagtccg aacacttctc cactgtctac ctcaaagctt
    13561 atgccgtcca ctgcctttgt accgccaaag cttttatgca gatttaccac ctttaacaac
    13621 gccatatcgt tacccctctt aatcctttat attggacagt gcctcaatct taaagggcat
    13681 gttctgttcg atattttcca tttccagagt attttcatct gtcagtgcag gaaagtcaaa
    13741 ttcctgatcc ttgtttatat cccagttttt tatatccaat tcatagctga ccttggttac
    13801 tgacgccggt tccgcattat caacctctaa tgtgattcca accttttcat ttactatata
    13861 tccgtcaata tccacatagg ccgtgtatcc gaagctttca acagtatact tcttaatatt
    13921 ctccttaatg tccgaaagca gtttttccaa agacgtatcc ttcaggaact ccacattctt
    13981 cctgatgtat ttcccgtagt tctcttcaag ctttttgtcc ttggaaagta tatctatgca
    14041 gttttccgcc aggcttttaa tctgctcatc attcaatctt atggtgtact cattagcctt
    14101 tacctccccg tcgggagtgg tgagtaccat atccttaccc ctgaatacat cctccctctt
    14161 aagaagccca agccactttt ctttaatcgc gtcctgtgtt tcctcggaaa taacctccgg
    14221 ttccccttcc ccctgtttat cgtccatgtg cttctccatt tcgtttaacc ttgtatactt
    14281 cccaacgacc ggcagcttca tgaatatttc ttctccgttt acgaagacat ccagatcaaa
    14341 gcctaagccg ccaaaaatca aatagtatct gaaaattccc ttttcaagtt catcgtcata
    14401 agcaacattg aagcttccct tcatattttt aaaatagtac agctccttta tctgctcttc
    14461 tgtcattccg gttgtgtcaa aatccattac cattgaaatt tccccttctg tccggccttt
    14521 ttttatctga tctgtttttt ccgccgcttt cctatagcct tcaagactat cgaagctgca
    14581 gccgataaat gccgctgtta gcaccaaagc taatatcgca atcattaatt ttttcactga
    14641 aaatccctcc aaaattatct tacgatatca ttatacagat tcaaaatcag catttcttgt
    14701 atcttaaatc acttattaag caatataccc catgactttt tgcacaatat atggataatg
    14761 cgtcgggggg acatggggac gtttcccctg tcctgctttc agcgggacac gcgaaacgtc
    14821 cccatgtccc cccgacgcat cattcttcag atacgtaaga aaagacggaa atgccgcctt
    14881 taaagatcat tctttatagt aaataccgcc agctgtgtcc tgtcccgcag gtccagctta
    14941 ataagcactc ttgtgatgtg gttcttaact gtgccttcgc ttaagtatag ctcatcagca
    15001 atttccctgt tgttctttcc ttccgcaatc agcctgcata tctctttttc acgatctgtc
    15061 agaagctccg ccctggaatc cttatgcttg gaccggtctc cccgggcaag ctctgagaat
    15121 ttgtccagca cctttaccgc caccgacgac tgtataagag cgccgccgtt atataccgta
    15181 cgcaccgccc cggcaatctc cgagggtgag gcatccttaa gcatatagcc tgaagcgccg
    15241 cttttgagcg catcatatat gtactcatcg tcattgaagg tagtaagaac tattatcttt
    15301 atatccggaa aatccttttt tatcataccg gtagcttcca caccgcttac ttccggcatc
    15361 ttgatatcca taagcaccac atcggggcgg aggcccctgc atgcttcgta agcctttccg
    15421 ccgttattgg cagtcccgat tatctgaata tcctcttctc ttccaagtat aagcttgagg
    15481 ccttccacca ggatgtcctg atcatcaacc aacagcacct taatcatttc ttaccctcca
    15541 agtatacgat accttttaaa tagaatccgt cgctgcttcc tgtttcaaac tttccgttga
    15601 aagcctccac ccgttccctg attcccttca aaccgtagcc ttcctttaaa gtctctgccc
    15661 ctatgccatt atcaactatg caaaatgtta tggattcatt ggaataatca agccggatcg
    15721 atatcctttc tgctttgcca tgccttacgg agttagtcat tgcttcctgg acaatatggt
    15781 aaaccgctat gttcgccgcc gaatcctgaa tgttatcctc accggaaatt tcaagcttta
    15841 tctccacacc ggtttttatt gaaaaatcat ttacgagctt ccttatgaat tcaaccggtg
    15901 caagggcagt accggttccc ctcagagtct caaccacctc tctgatgctt gccatgctgt
    15961 ccttggcggt ttttaccgag cttaccagca gatcctccga ctttttggga tcttccttca
    16021 aaagatgctc agccatctgc agctgcatga taagggcagt catattatgg cccaaggtat
    16081 catgtatatc ccttgctatg cggtttcttt catcaaccac cgaaagccgg ttcacttcat
    16141 cggtatactg tttcagcttt ttatgcgcat ccagaagctc cctgtagagt atatccttcc
    16201 gctccctttc ttccttgttg tgctgtgcaa aacccgcaac aaccagtata agaacattga
    16261 ccataaggaa aaaaaccagc tgggatacgt ttgaaaggtt gaaattatag taaatcaaat
    16321 atatgtattt tatcagagat accacgactg ttgcagcccc gatcattata ccccttctga
    16381 ggcccagcag caaggatgac tccaacaata ccgctatata aaaggaatgg aaaaagtagt
    16441 ttatcaggag tcttgaattg tattcaagag tatataccaa tgctatatca aggataaagg
    16501 acaggaagta caaattgtcg taacgcctaa tcaagcctct tccgaagcca accgccaggt
    16561 acagtgcaaa tatgaataca agcgcatata gcctctgccg ctgcgcattt tcaaaatata
    16621 ttgcagtaca gaacaacaag gtaaataata tggctttaat aatgctaaag agccttctcc
    16681 ctttttccat accgataccc ttccattgtt ttgaattcat gaaaatatta tacaatatca
    16741 gatgcattat atccatgggt tattattata tgcgcagtat gccctcaaat catcagcatt
    16801 ttttaggtta tccgcatatc cccatgctca ttattcaata tacttatgct cacagttttc
    16861 tttatcagcg aaattgcatt tatagcggca gttctttatg ctgcattctt tacagcaaat
    16921 gttacccata tcaaagcagc ttgatatgtc acacatgaag cattttctca caacacgtcc
    16981 attggacttt tcgtcctttt cattaactgt aaataatttt cttgcaatct cgcaagcatt
    17041 atatatcacg gtattctgtc caaagtccaa gcaaatacct ccccgaataa taatagacat
    17101 gtagtccaac aacaatatta tcttactgga ctacatgtcc actgtcaaca atttttgccc
    17161 ctatttgtcc atttttagtc cgaattggac gaattgtgtt aacatcatac tataaaaccg
    17221 caaaaattaa tgcaatattt tgaggtgtat tcccatggaa tttggtgaaa gattaaaaga
    17281 tctgagagaa gaaaaaaatt tgacaaggca tttgctcgct agtatattga atgtttctta
    17341 ttcgacagta tcaaagtatg aaactaatat aagatttccg gataaggata cattagtggc
    17401 attggcggat ttttttgatg tgtccctgga ctatttgctc tgcagaagtg atattaagga
    17461 taccgcagac aaggcactca gcaagtgcaa acagcaattc atggttacct cttcggggaa
    17521 catatataat ccctatgagg gcttatctcc ggaggctgtt gaggaaattg agaagttcaa
    17581 ggaattcgta cagcataagt atggaaagaa gtaggcgaag ctacactgtc tacatggata
    17641 tacacagtaa cctatcactc agtaatgcat tactgccaaa aggataagat ttattctaca
    17701 aaattcctgc gcgactattt tcacggggat tcccttaagg taccctatga tattgactat
    17761 gacaaatcta tatggataaa agaaatgtgt gacaagtgcc tgacaggcat acttcattgc
    17821 ctcgaccgtg aaagccgttt ggcatatttg ttcagggatg ttgcacagct ttcctgcgaa
    17881 gagattgccg agattatgga acagaaccct gcggcaataa ggaaaacagt atcccgcagc
    17941 agaaaaaagc tcaggaattt tctgaacaac gaatgtgttc tgttcaatcc ttcaggcagc
    18001 tgtaaatgcc gcatgaagaa tcttgtggaa gacattatgc tgccccggga gtatcaggaa
    18061 ttaaagcgtt ttgtatccag agtcaatctc tacaaggagt cccaaagtat actgcctcaa
    18121 aaaaattatt ggatcagtaa ttaaaaatgt gtcacaattt caccctgacc ttctctaata
    18181 ctaatgaaaa cctttaacgg gtattaaaaa aacgggggcg tacttgaaaa ggtaactgaa
    18241 caaaagaaaa aagcttgcga gttacaaggc tcgcaagttt tttcaaaatt ccaataccaa
    18301 tcctactcct acttcttcaa gctccgccgc ctccgataat gccagctttg atttcaggtc
    18361 ggtgcaatgg caggcatgaa gcttttttac acctgcctgt ttgatatatt tttttgtcag
    18421 ttccaggact tcttcttcag ggctcaatag gtgaagccct cctataacat ccgcaatcct
    18481 gtcgtcattg cagacttttt ttgcatattc tattatattg catatgcctg aatgggagca
    18541 tccggtaata ataaccaacc ccttctccga tttataagcc aaggcggtat catccctcag
    18601 gaaatccggt tcttcattgc cttgaaccgc acgcttccct ataggctttt tattctcaaa
    18661 gctgtttgtc ctttctattt cgcccaggaa taccagccta tccgtgatcc aaaagggctc
    18721 ccttttcaaa ttcatatcaa aaaaatctcc cagcgccttt tcagagatta cggaacctat
    18781 tacttcatta tcatgatact tgctttcaaa agcctgcgga tgggcaataa gaacaggctt
    18841 cctgaagccg attttttcaa tcatcgcctc cgaatacatt cttatcatgg gctcaagccc
    18901 ccaggtgtgg tcaatatgcc catgggatat caccgcataa tccacatcat acaggctgat
    18961 tccaagcttt ccggcattgg tgataaatat gtccgagtaa cccgcatcga acagaatgtt
    19021 tttcccatcc gcacggataa aaaaggacac accgggttcc cccagcagat aacggtcaat
    19081 tatcgtattg ttgtcaacca gaactgtgat ttgcataaat ccagcctccc attcatttat
    19141 ccatttacta aaattccatt gcttctataa taaaaaacct tcctgttatt aacaacagga
    19201 aggtccaaat tctttaatac cccagttctt tattcacaat aattacatgc attcttccct
    19261 ttacaccctg tcttctgatt atcgtatatt cattgcagat tcttccttcg gaattacaat
    19321 ccatgcaatg tcccgttttt gtacatggat tggttttatt cagccgttta ttattggcag
    19381 gagccgctgt ttcttcgact cgtaaaaccg cttcttcaat atccttgaca agtttgttta
    19441 tccctacaat aacgataacc cggtcaggcc catacagcat agaagcaacc ctgttgccgt
    19501 tcgcgtccac attatagagt tctccgtttt cggtaacagc attgctgctt gtcaaatagg
    19561 catctgcaaa aaatcccttc ctgaggattt ccttcatttc cgacggggtc aagccttcct
    19621 tgtacctgtc caaaacgtta taattgccct ttcttatata atcaatagca cccacctcaa
    19681 aaagcgtcat agagccgccg atgcctatcg tacttccttc cggtattagc tcgccaacct
    19741 ttttcaatgc ttcctcttcg ttttcaaccc tataagccga gatattgttc ttttccagat
    19801 tttctacagt cctttttatc ctctcatcaa ctacaaattt tacgttttta tccattatac
    19861 atcttctcct ttcttatcac accatcctga tagcaaagaa ccggcaggac ggaatacaat
    19921 acccgcattg tatacatttt gactgatcaa tgtatgcttt gcccgtatga tcgaagccgg
    19981 gggcaccgct atggcatact tcaatgcact tgccgcagct ggtgcatacc gtctgtgcaa
    20041 tttttatgct tttctttttc ttgattttta tgatgtcatc caaatctttt tccccgttaa
    20101 aatattttac attgtacatg acctcttccc tgcttaccat tccgacagct atagaatggc
    20161 agtaatccaa ccttctgcca tattccacgc agtctttgta ctcatctatt aaagtaccgc
    20221 cccctaatac tttcatcaga tatatgccct tcccgttttg cccgcacagg gatatggcgc
    20281 ccctcatttc ttcctctgtt ccttccaata tcccccggcc tgctttgtta agaagcggaa
    20341 aaacaatatc aatatctttt cttgctgctg cggcttctac tacttttaca ttgtgcgtcg
    20401 atatccccac tgccttaatt atccctctgc ttttatagtc tagcaggcac tgcaaagcac
    20461 ctttccgtat ttcaaaaaca tcggtgtcga cttttgctgc atgaagatga aaaatgtcaa
    20521 tatagtctat acccattcca tccagtgcct cttttatggc tttttccatt tcttcataag
    20581 atgcagctgt agactttgtt gcaattaccg gtctgatccc cgttctttca atagctgatt
    20641 ttatcggcaa atatgttctg tacaactgtg ccgtatccac aaatgttatc ccgctttcca
    20701 gggcatatgc aacaatttca gcagattcct catccggaag gttcttttgg aggggtccca
    20761 taggcagtgc tccaaagcat aattcagtaa cttcaattcc tgtatttcct aaaacgttct
    20821 ttttcatgta gtcatctcct tacattgtta cttaaatata ttcaatcact aatcttaact
    20881 tttaaatggt ggacgatagg gggttcgaac ccccgacctc tgcgttgcga acgcagcgct
    20941 ctcccaactg agctaatcgc cctccctccc aataatcatt atacattata atcttcaaag
    21001 ctgccagcag gatataacgg aaatcttgca atcaatgctt cagccctttc tctgcaagcc
    21061 tccagatttg cttcgtcttc cggtgcggct gccacttggt tcattatttc tgcaatttct
    21121 ttaacttcaa cttctttcaa tccccgctgg gttattgacg taactccgat tcttacaccg
    21181 ctggtaacaa gcggcgacgc aggatcaaag ggtatctggt tcttattgac ggttattcct
    21241 acttcatcaa gagcatcctg gaacagcttt ccgcttattc ctttggatct caaatctacc
    21301 attacaatat gattgtccgt tgtgccgctg acaattcgga acccaagacg ggacagctct
    21361 tctgccagag tcttcgcatt cacaagtatc tgcttcataa tactgcggaa ttctgcagac
    21421 gccgcatact tcatcgacca tgcttttgcc gccattgttg ttaaatgcat tgatcccagc
    21481 gaccccggga agaccccttt gtcaatcagc tttgcatatt ccttcttgca gagaataaat
    21541 ccgcttctgg gtccgcaaaa agtctttgta gtcgatgatg ttacaaaatc ggcgtgaggc
    21601 accggactgg gtatgacttt tgccgccacc aacccgctta tgtgtgccat gtcaaccata
    21661 aaataagccc ccgcggcttt tgcaattttg cttattcttt cgtagtcaat cagtctcggg
    21721 tatgaactgc ctcctgctat aatcaatctg ggttttattt cttccgcctt tttttccagt
    21781 tcatcataat ctatcatttc tgtttcggga tttacaccgt aaaaatcata ttcatacacc
    21841 tttgaaagaa aattcgcagg acttccatgg gtcagatgcc ccccctggtc aagcctcatg
    21901 cttaggacct tgtctccggg cttgagaacc gccgcataga ctccataatt tgccgtggag
    21961 ccggaataaa cctgaaaatt cacatgctcc gccccgtata acttttttgc ccgctcaatc
    22021 cccagaagct ccaatctgtc cgcctcatgg gagcctgcct gaaagcgtgc tcccgggtag
    22081 ccctccaggg ttttattcgt aaatatgcat cctgacagct ccataacctc caaaggtgcc
    22141 gtactttcag aggcgatcat ttcgatatta tgctcctggc gtcttaattc atcagcaact
    22201 atttcataga tctcagggtc tgtaattcgg gtacgcttta acatttccat ccctctttcc
    22261 aataatgttc gcattcgctt actgctgatt ttaaagatta cctttcacgc tttactcaat
    22321 aattatctac aatatacaac aaatgaccgt atttgaaaag gctcaatgct ttagaaaata
    22381 ttcccctctg tgctataatt caagtatacg gaacctgatg accttgatga aataaggagg
    22441 gtactttatg cggtttgaaa aactatatgc ggatcctatt attgaagacc cgggatttcc
    22501 tcctgaaagt atggctgttg ttattaacag cggcggcaag aatctttatg gtgttttgta
    22561 cacagcagcg ggcatgggcc ctcatccttc catgattctg cttcacggct tcccgggtac
    22621 cgagaggaat ctcgatatgg cccatgcttt caggcgggca ggctggaaca ccctggtgtt
    22681 tcattacagg ggttcctggg gcagcgaagg atgcttctcc tttcaaaacg tgctggatga
    22741 tgtaaaagca gcgatagagt tcgtaaaatc agaggaacct gcccgaaaat accggattga
    22801 cagagataat atagtccttg tcggacacag catgggaggc tttgccgcac tgctgactgc
    22861 cgctgatgat atggatatca aggcatatgc tgcaatagcc ccatttgact tcggcgctat
    22921 aggggaaaac tccgaaggag atgataaagc tctgagattt ttgcagggaa tgttcaagga
    22981 gtgtattgtc ccccttaagg gtaatacagt tgaggaactg attaatgagg cgatggcaaa
    23041 cggtaataaa tggagcttca tcaacaacgc ggaaaagctg gcaaaacata agctcttatt
    23101 aatagctgcc aaaagggaca acctttccat tccggagctt cactactatc ccttggtaaa
    23161 taaaatgctc aagtataata ccgaaaactt tgaatataaa atgattgatt ccgatcattc
    23221 ctttcaggat aagagaatcc tgttaactga aataattgaa ggctggctgg aaaaacaagt
    23281 tcagcaaaag agtaacataa aataaatttt aggcatatca tgataaatat atgaataatt
    23341 attgttaatt agcaggatat tcctgttaaa cagcgaatta atcagtaaaa taaactccaa
    23401 aaaaaaggag gatggaatat gagtttttta aaaaatcttt cggaaaaagt cgttagtacg
    23461 gcaaaagtag taggtgacaa atcacaggac atggtggagg taagcaagct gaagcttcat
    23521 atttcacaga ttgagggaga tattaagaaa ctgaagttca atatgggcga gctggtttac
    23581 aatgcatttg cctcagacac tgaattcccg gctgaagaag taacccgcat cggagaagag
    23641 ataaaagcaa agtatacgga aatagaagag accaagataa agattcagga agtgcaggct
    23701 aattaagtta taggacaatt gagacagggg ggacgtttct cttgtcccgc aagcgggaca
    23761 agagaaacgt ccccctgtct cagtgagtgc taaaatgctg tatccagctt atacttcaaa
    23821 gcattttcct tatcagccag gggaggaact gagccttcct ctccaagcag cagatacttc
    23881 cctgagtcgt ccgttcccgc ggtgaaatat gcggaggtgt cagtcagcgt ttctcttgct
    23941 ccccaggtct ttcctccgtc ctccttaaat gtgtacattt gtccaaggcc tgatgcaccg
    24001 ccttcgtact ttcctgtaac tgcgttgaat tgtccccctg acagagtgcc gagaatccat
    24061 gtcccgacga cagaagctga agcaatcgga accgcggctg ccggggcagc ctttatggta
    24121 tccatctgcc cataagtcct cacgctcatc agtattgcag cctccctggt ggccatgccg
    24181 tagccggctg cttcctgtgc agttgttgtc gccttgggca taaaatatcc cttggcatca
    24241 cctttgacta tagccagctt tgacatgtat tttgccgcct caaccgcata gttggccata
    24301 tccttctgat cgggaaaatc cgggataccc gctacgctgt aatccccgtt ggggtgaatg
    24361 gccttaatgg cacggaacag catggcagca cactgttccc ggttaatcaa aacctggggt
    24421 gaaaaggtag tagcgctggt gcctgttgtt atgccgagag caaaggcctt aagcacctga
    24481 gggtttttcg tgtctgtaaa ggggttttga gaagcaggtg cagggctttt gccgatactt
    24541 caaggtactc ccaagttgcc cattcatagt gaatctgctt gaaatccttg tccccggctc
    24601 ttctcatcca tatctctgta cgcatggagc cggaggccat tgaattcaga tcctggactt
    24661 cccccggaac cttatccagc tttacttgga aatacggctc cccgtccggt ttgagtttcg
    24721 cttctgccga caagatagtg ggggcatgat ttatgagctt attgtaatca gcctttgtat
    24781 tggcagaaag agtgaattcc cttgaccagg gcgacaatac ggttgtctct ccataatcaa
    24841 aggattctgc aaagcgcgcc ctgaaggtga cggaatggct tttcaggtag tcccagccgc
    24901 cctcgtagaa agccttcagc tcctcatcct cctggaataa ggatgacatg ctccatcttt
    24961 cactgttgtt atacaatttg ccattgataa aggtcatgta catatcatcc agtccgtcag
    25021 gaactgtttc gcttgaatcc caggctgatg tatgatgcca ttagaactgc aagtaatcta
    25081 cctaccactg tacattcctc ccaaaatttt atttcatgcg atccaaggta ctcgcattgt
    25141 cagcttattt taaatatttc ctttgtcttc aggtaacttt atcatccctg ccgataaggc
    25201 ggggaatata tactctctgc ctgtaaataa aactgggatc aatccctact catctttttg
    25261 aactgaaatt cccattgttc cgtccttgac cataaaattg agggcaacgg taatctgatt
    25321 cccgtcattt ccgccgaaaa taatcatttc atcggttctt gattcataaa catcctgtgt
    25381 atatgttttc ttgatttcct tcagataagc ctcaaaatca tcagctttaa cctcatccat
    25441 aataaccatg atgttattgt cctggaaagc cgaggttgct atctttccct ttttaaactc
    25501 cggaatcttc tttgccaaat ccgtatccgg ccacttcccg cctccgactg taatttcttc
    25561 tccgtcttcc cctttgactg tcactgtatc acctttaatg tctacttcgt caccgagtgc
    25621 tttctctact attttctcag atattgcttg ttcggccttt ttctttatgc cgcagccgcc
    25681 aagcggcagt attactgcca acaagcagct gcataccagt aaacatgcca atattctttt
    25741 catttatttc tcctcctcta ataatttatt tcgccattac ggcaaataat tagatgttta
    25801 ctcaagatgc ttgttgaaat tttcaacatc atctccccct tcttttttca gcacattcaa
    25861 tgcaaagatc ctgatccggc atttattgaa gcttacttct attatgaaaa ataagtcttc
    25921 aactgtatat caccctaggg gtgattttcc aaaaatttca ataaaaaaac cccacctgat
    25981 taaggtgagg gaacagccaa gcctttaggg cctagttcaa ttcggaaagc ctgccgcttt
    26041 tttccataag gcttatatag agggaaagct cgttccgtga agatacattc agctttgtgt
    26101 atatatgatt attgtgtgtt ttcaaagtat tggcactaat atacatgagc tcaagtattt
    26161 cgccgttgga ttttccttca agataatacc ggaatatcat cttttcggta ggtgtcaggg
    26221 ttttcaccct cccgatgaaa tcggtgaata tatcgtcggg cagcggagcc tttcctgtac
    26281 gcctggcttt tatctgcccg ataatttcat ctatttcagt tatgttggtt ctggctatgc
    26341 tgtcgatatc ctgggagcct atggcattaa gccccttcat aatagggttc atgtatttat
    26401 tgcctataaa taccgaagca gcggtaccgg ccagtaaaaa caacgcacac aacactataa
    26461 ggcggatatt catccaagcc aggcgccggc ccagatcctc ctggggcatt aaaataccca
    26521 cagcaaaatg ttcttccgca aaagctgaat cctcagggta caaccggatt tctttatgca
    26581 atcccgccag gcttaatcca ttctgctgct cataggaata gagcaatcgg ccccggtttt
    26641 ttataataag gggcttttta tccccggaaa cgccaaaggc atcgtaattt ccggagaaaa
    26701 gcgcactccc cacccgcagg accgaatcct caatcggggc aagcatgcag aatatgcgtt
    26761 gatacgcgga gctgtcgggt atgaatttga gtttgaacag catggcgctc acttcgtagc
    26821 cgcatactcc gaaaatattt ccgcttgaat caataagagg cacaatacat agcattgcat
    26881 cctcgctggt ttttggcagt gtcacagccc cgctccagta atacagccga gaaagaggac
    26941 gtggcttttc cttcgccttc tcaaagggca gaaaaaaatg cgaaggactg tctatcataa
    27001 attccatgtc ccattgggca tggagagaat acccgcggtc ccttgcaata tccggaaagc
    27061 cccttaaaat agtaacatca ggcggagcgg aacttatgat attgggttcc atgtttttca
    27121 gatagagccc tgcccgggaa aactccgcag cttcggcttg cttgttgacg gtggcatcca
    27181 aaacaataaa agcgccgctt gcgccggtct tttccaaaga aaataacagc cggtcaaatt
    27241 ccctgtccag cagctcttcc aaaacctccg gagcttcagg caaatcagcg gtactgagtc
    27301 ccttttccga aaggaacctc tccatgcttt gggacaggga agaggacagg cttacggctg
    27361 aagcggaaac atggccgtat tccttttcca cagctgagga tattctctta aactcattgg
    27421 aatagaaacc ctcggcctcc ctgagccctg cggtaaaggt ccccgttaaa agcagtatta
    27481 taaaaacaaa gctcagcaag gtcaggacaa gcacaaacaa aaatccaaac agcctnnnnn
    27541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntaccc ccttcaaata ccggataggg
    27661 taaaatagcg tattccgcat tttccttggt attgtccgca taggtaacag tagaggggta
    27721 aaacagtact ccggcagtgg agcctgtgga gcagacaata tcgccggtct tgaacagatc
    27781 ggtgctgtac ccgtcgaata tggccgtgct tcctttgaca gcggaaagga aatatgtatc
    27841 ccatattttt ttatataccc ggccggctgt attcagcccg ttattttgca caaaatcctc
    27901 cccaagctgg ttcatgccta taattgccat attaaacacc gagtcgggca tataaaaagc
    27961 tttaccctcc gaccattcat ggtatttatc cgccgtcctc atgattccct caaaggtgga
    28021 aagctcgtca taggttgagc cggtatctgc cgcgaagcgg tcaaagatgc ttttgtttac
    28081 aaacagagcc tcggtggatt tggctatggg aaaaacgaag agccctccct tcagcctgcc
    28141 ttcttccaca aaacgcggca cataagccga cagctcctcc tctgtaaacc ggccggataa
    28201 atccaccagc aggtcctttt cgaccagctt cagcgcagtc tttgggtata cgacggcaag
    28261 atcaggaagc tcaggggctc ccgggtcgcc gaaggctgcc gatataagct tgtcatgcag
    28321 cgcacttgaa ccggagattg aggtcacact tattataata cccttttccg ttcccacggt
    28381 acggttgaat tcatctatca tctcatccat gaggtttttc atctgcccgc cgtagttgtg
    28441 ccacatggtt aaagtgacgg ggcttttagg attcagtacg gtttttgtac tgcagcccac
    28501 agagcataaa agaatcagaa aaatcagcat cagacacaat gcccgaacct tcatcatacc
    28561 acccctcttt tgcttttatt ataccattta attttataat acaatcatta aaataacata
    28621 aaatgcaagt tccggaactt gaaacataat taaaaggtgg tctaattctt acgaatagac
    28681 cacctcaaga accaggctct aattcaacgg aatacctttg aatctaccaa atgttaacta
    28741 cctcaatcct ttcaaaccaa aaatcttacc aaacaaactc taaaccaatt ttaccaacta
    28801 cacgaactac attccaaaac tcaaaccaaa tgggtcacag tactttaggg aaattgcaca
    28861 ttagttgaat tagagcctgt gggaaagatg taatcatctt tcttaattta attataccac
    28921 aaaataccca taatgcaatg ctaattttca gaatatttaa atttttactt gtccatgagc
    28981 tttgacggct atatcggcag gaaacataat atccgacgga gaagtataat gtcaatatat
    29041 tggaaaaact ctaacttaat ggagtggtta aagtgaaaat atcggttgaa gatgtaagat
    29101 atattgcaaa gcttgccaag cttagtataa ccttcgaaga agcacagaaa atggtcgggg
    29161 aatttgaagg tatactgaca tatttcgaat ccattgacag gcttgattca ggggatgccc
    29221 tgccggatcc gccggagcgg gaaatggcta cggttctgag aaaggatgaa atctctgttt
    29281 ttgaagataa ggaaaaactc tttctgaatg ccaaatccat gagggattcc tatatcaggg
    29341 tgccaaaaat tattgaatag gtggcgagcg ggttgaacat atatgaaatg actgcactgg
    29401 aaatccgaaa aggaataacg gaaaagcaat tttctgcaat tgatgcagtg acggcatttt
    29461 ttgaaagaat ctgcgaaaag gaaccgttgg ttgacggttt tttgaccctt tgccgggagg
    29521 aagccctggc taaagcaagg cagatagata gaaaaataaa agcgggagaa ccgctgggaa
    29581 aacttgcggg gattcccgtt gccataaagg ataacatatg caccgacgac ataaggacca
    29641 cctgcgcctc cgggatgctt caggatttca tcccccccca tgatgccgcg gttgtaaaaa
    29701 agctgaagga agccgatgcc gtaataatcg gcaaaacgaa catggatgag tttgccatgg
    29761 gttcttccac cgaaaactcg gccttcaaaa agaccagaaa cccctgggac agagaaagag
    29821 tccccggcgg ttcttccgga ggctccgcgg cagttgtgtc agccggaatg gctgcccttg
    29881 ccctgggctc cgacaccgga gggtctatcc ggcagcccgc tgccttctgc ggcgtggtcg
    29941 gcctgaagcc tacctacggg ctggtttcaa gattcggact cattgccttc ggatcatccc
    30001 tggatcaggc cggccctatg gcaaacaata taaaagactg cgctttagcc cttgaagcca
    30061 ttcaggggga agacaagagg gacagcacct ctgtcaggca ttccgttacg gaagattaca
    30121 tgagcacaat cgaaaagggt gtcaaaggca tgaaaatcgg tgttccagcc gagttcttcc
    30181 gctccggcct ggatgaagaa attgccttct acatcaaagc atgtatgcaa aaattcaagg
    30241 aaatgggtgc atctgtggag gatatttccc ttccgataac agaagaaggg ctctccgcat
    30301 attatataat ttcttcggcg gaagcaagct caaacctggg gagatttgac ggcctgagat
    30361 acggatacag aacagaaaat ttcaacggcc ttgaccaact gattgaaaaa agcaggagcg
    30421 agggcttcgg ggaagaggta aaaagaagaa taatgctggg cacctttgtc ctgtcctcag
    30481 gatattatga tgcttactac aacagggcac agaagctccg cgcaaggata agggaacaat
    30541 tcattaaagc ttttgaaagc tatgacctct taataagccc cgcttctccc gtactgcctt
    30601 tcaaaatcgg ggaaaaggcc ggcaatcccc tggaaatgta tttatcggac atttataccg
    30661 taaatataaa cctggcaggc cttccggccc tttccatgcc ttgcggcata agccgtgacg
    30721 gactgcccgt cgg
//
LOCUS       DAOQUR010000027        29842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1819_length_29842_cov_9.742740, whole genome shotgun sequence.
ACCESSION   DAOQUR010000027 DAOQUR010000000
VERSION     DAOQUR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 29842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29842
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_29842_cov_9.742740"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..699)
                     /locus_tag="PKI50_07245"
     CDS             complement(88..699)
                     /locus_tag="PKI50_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNT03533.1"
                     /translation="MNESVKKTIEALEKHNMKASYFDDSDTAVSYLMGKIGDGATVGI
                     GGSMTVYSLGIPQKLIERGNRVYFHWLESTPEGMDYARDKAQKADVYLSGTNALTEDG
                     KLVNIDGLGNRVASMIFGPKKVFVLCGVNKITKDLDAALKRIKENTYKNARRLKLDTP
                     CAHTEKCNDCNSPQRMCSVTTIIERRPYKTEFEVIIIGKELGY"
     gene            complement(752..2185)
                     /gene="glnA"
                     /locus_tag="PKI50_07250"
     CDS             complement(752..2185)
                     /gene="glnA"
                     /locus_tag="PKI50_07250"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNT03534.1"
                     /translation="MFKNLTEVKEFCNSKGIKIVDFKITDLAGRWHHLSIPVTRLNEK
                     VLEEGIGFDGSNYGFKTVEKSDMVFIPDLNTAFVDPFCEIPTLSMIGDIFALEGGIRR
                     YEGDPRYIAEKAEKFMASTGIADENIIGPEFEFYLFDQIAYESRPEHQEVAIDSAQAE
                     WNTNDQYEKNLGYKVRHKGGYHLALPYDLTNDLRSKMTVMLEEFGMPIKYHHHEVGGP
                     AQVELEVEPGKLKDMADKTYLLKYVVKNIAIQNGKSATFMPKPIFGEAGSGMHVHMWL
                     YKDGKPLFYDKDGYSGLSQTALYFIGGLLKHSPALLGFTSPSTNSYKRLVPGYEAPVS
                     ICFATANRSAIVRIPGYGNTPDSKRFEFRSSDATCNPYLAYSALLMAGLDGIINKIDP
                     VKEGFGPYDVNIFELDDEEKKKIKGLPKSLEEAMEALRQDHEFLLKGGVFTKSLIETW
                     IKNKIEKEHKKISIIPHPAEFELYYDL"
     gene            complement(2282..3172)
                     /locus_tag="PKI50_07255"
     CDS             complement(2282..3172)
                     /locus_tag="PKI50_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNT03535.1"
                     /translation="MSAKGKIITAVVVAVLLIAAAIYGFTYNFLSKISVEEGAEGYPV
                     PEKRVNVVVMGVAKGLADTIMLASFDPEDGALDVLSIPRDTYIERKGHKISANNKINS
                     SYGRGGADNVAESVVSLTGLDVHYFAEVDYEAVKELVDAMGGIKVTVPMDMNYDDPVD
                     DLHIHFKKGEVVSKGEDILKLLRYRKNNKGGGYKEGDLGRVKMQQEIVRLGIDKVMRG
                     NIAANFLKLQEPVKEYVKTNMPPKQMMYYITKAKKIKKENITIQTIPGRAETINKLSF
                     FVVNRDKMDEVLKPLIEESK"
     gene            complement(3308..3568)
                     /locus_tag="PKI50_07260"
     CDS             complement(3308..3568)
                     /locus_tag="PKI50_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNT03536.1"
                     /translation="MNEYYVAVFDSTHYALKFEKAVRDWGFNISIMPVPREITASCGI
                     SAKLKADEIENIMALAVKNDLKVSGYYHVEVQNGRKVYHKST"
     gene            complement(3592..4101)
                     /locus_tag="PKI50_07265"
     CDS             complement(3592..4101)
                     /locus_tag="PKI50_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT03537.1"
                     /translation="MNKNVELWDILDEDGRLTGRTIERGKPLKKGEYHLIVHIWIRNS
                     KGEYLIQKRSSQAGAWPGVWGAAGGSAISGESSLDAALREVKEELGLSFEPDALKRLY
                     RVRYKSVFSDLWFVCRDINPEDVRIQKEEVSEVKYACMDEILSMMESNEFINFGEAYM
                     SFLVNPCNC"
     gene            complement(4223..5005)
                     /gene="aroD"
                     /locus_tag="PKI50_07270"
     CDS             complement(4223..5005)
                     /gene="aroD"
                     /locus_tag="PKI50_07270"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNT03538.1"
                     /translation="MRLQAKKPIKIGNRTIGGPDMLICIPILEEEESGLERAAKNTIQ
                     LCPDIIEWRADYFKDACSPIKVMKALNMLRSIIGEIPLIFTFRGSFEGGFIEVEENIR
                     YEIIRLVLCTGEVDAADIELASRKNAVEEIRKEAYDHDIPLIISYHNFDETPSVEFLL
                     NKIREEVALGADIAKIAVMPKNEEDVLKLLSATLKARIELPYTPLITVSMGALGIISR
                     IAGGMFGSDMTFGAVEKTSAPGQIPIAELRASIKTLLYRIRR"
     gene            complement(5051..5500)
                     /locus_tag="PKI50_07275"
     CDS             complement(5051..5500)
                     /locus_tag="PKI50_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HNT03539.1"
                     /translation="MNILVSACLLGTNCKYNGGNNFTPKLLELKRENNIIPVCPEELG
                     GLLTPREPCEIQEGSGKEVLEGKSRVINKIGEDVTADFIKGAGETLALAKQHNCRLAV
                     LKSRSPSCGCGIIYDGTFSGKLKEGNGVTAQLLLDNKIVVMTEKDFE"
     gene            complement(5514..7859)
                     /locus_tag="PKI50_07280"
     CDS             complement(5514..7859)
                     /locus_tag="PKI50_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNT03540.1"
                     /translation="MKDKALRILEYNKIIDMLCENTVSPMGRDIAADLKPSGDLNEIK
                     ELLQETTEAVNLTLKKGSIPIGGVQDIRMPLKKVKMGSALEPGELLKVADTLRAARRL
                     KAFMNEDRGEESFPIIEYYIDTLSAFKGIEDRINSAIISEEEIADNASPQLSTIRRKI
                     KEKTASMREKLNHMISSPAYQKYLQEPIITVRGDRHVIPVKQEFRANIPGIVHDQSAS
                     GATLFIEPMAIVEMNNDVKKLKLDEENEIERILRELTALVDEKYDNIKTNMEILATLD
                     FIFAKARFSLDFRCTEPRVNNKGIVNIKKARHPLLKAESVVPIDIYLGETFNTLVITG
                     PNTGGKTVTLKTTGLLTLMAQSGLHIPANDYSEVAVFDEVFADIGDEQSIEQSLSTFS
                     SHMTNIVSILEHVTPNSLVLFDELGAGTDPTEGAALAMSILELLHKKNIRTAATTHYS
                     ELKIYALTTEGICNASVEFDVATLRPTYRLLIGIPGKSNAFEISKKLGLSEEIIKRAK
                     EFISQDDVKFEDLIKSLEEDKRKAEEERDVASRLKFELEKEKSEMDRRLEKLEAQKDR
                     IVREARSEALEIVRRSKEEAEELIRELRDAAMIEKSRMKLKQRENEIEDKIGESLFKK
                     TGSAPLKDIKIGMTVKIVNLDRKGSVLTLPDEDGNLTVQAGIMKINVNVNNLTAAEEE
                     KEKPKRSSGVKVNSAKAMAVASQIDLRGQTLDEALMNVDKYLDDAYLGNLPQVTIIHG
                     KGTGVLRAGIMQLLKSHSHVKSYRSGGFGEGGIGATIVEIK"
     gene            complement(7908..9389)
                     /locus_tag="PKI50_07285"
     CDS             complement(7908..9389)
                     /locus_tag="PKI50_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03541.1"
                     /translation="MRSYGIAFKLWASIIAMTLALLVFTLIFQTEFLNDFYFELEKEQ
                     LEKDCFRLANYVARDQHFTIPYYSVMRRVNDIIIITGNDGKIIYVEGTNKYKLDSIFG
                     KNHIGRILKGEPVYEQSKEISNPHSVWPKEMDTLLVGVPVKKNVVLTQENRQRISENY
                     EGGPLKQHEITGAVYIITTLEYMQTTIDAIRKQFLYIFITAIVLASAISYFLSQSFSR
                     PLKLINNAALEISKGNYNTKIDLRSSREIKELGDTINNLAKQLTRVEQIRREFIANVS
                     HEIRTPLSYLQGYSEVLQDGLVESEEERNKYLGIIMDETARLKRMVDEILQLSQMEAG
                     YVQLQSMPFSMDGMIRSMIDKMLPHALKRNIGIKFTKTPEDALNCFGDENRIKQVLIN
                     LIYNAIKHSYDNGEILISAYRQGENIYVCVRDFGEGIPEVDLPFIWDRFYIVDKKRSE
                     NSTGLGLAIVKNIINAHGCEINVKSVNGEGSEFCFYLPAHKEM"
     gene            complement(9386..10072)
                     /locus_tag="PKI50_07290"
     CDS             complement(9386..10072)
                     /locus_tag="PKI50_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT03542.1"
                     /translation="MNNSVRVLLIEDEEKMRELIKIVFRKEDFVIIEAVDGKQGLNLF
                     RTNSVDIVILDIMLPEIDGWTVCREIRRTSNIPIILLTARGEEFDKLFGFELGADDYL
                     VKPFSPKELMARVKALLRRAEIKSNEAVSYYRFGDIVIDRLSREVTVNGRPVNLTNKE
                     YELLYFLAANPKIVFTREQLLLKVWGYEQYGDPRTVDTHIKKLREKLGDYSSCISTMW
                     GVGYKFEVPK"
     gene            complement(10088..12562)
                     /locus_tag="PKI50_07295"
     CDS             complement(10088..12562)
                     /locus_tag="PKI50_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNT03543.1"
                     /translation="MNRTELLAPAGNFEALAAAVESGADAVYLGGNKFNARAYADNFD
                     GQSLTKAVNYAHIRGVRVFVTVNILLSDRELAEALDYVNFLYQIGVDGIIVQDIALLK
                     LARNAFPDLPVHCSTQMTVHNAEGARYYSELGAKRIVLARELTLEEVRGIAESTDAEL
                     EIFIHGALCVSYSGQCLMSSLIGGRSGNRGRCAQPCRKKYSLYNFNTGKAVIENRHKH
                     LLSTRDLNTYNRLKELTESGVASLKIEGRMKKPEYVAIVVRHYRNALDNIIKGEAFDT
                     ADAAYELKSAFNREFTEGYLFNRRNRDVVSIDRPDNRGVRLGKAIRQKGEIVSLLIEE
                     NSLHDGDGIEIISKSGGSTGAIISGIRVMGRNVKAAVKGDIAEVFIRDRVEAGSRVNK
                     TFDSELSRRAREEYSPENTRKIPVRCSLTVKTGEYPVITVNDDDGNTVSYTDEERAEA
                     AAKAATPIEKILEYLGKTGDTPYIIDVTETIIDGDCYIPARQINRMRRKVLELLSRIR
                     SQKYDRPIIQINAAELINGLISPVKASGEDSLEYIAGIRNYASAVSALNSGADSVYIL
                     SDVYEGDIVSETVKIAETCRSMGKTAFYVLPNIMTDRETGKIKERLEEIIKAADMRYF
                     GVVVSDAGQLKLARNSGIRRVRVNYSMNVFNSVSASHWHREGAEAVGLSAELNLPQIK
                     AITSNTAAAAESVVYGYLPVMTTEYCPVSMFRDNCNYPRGCNSSNYGIIDEKGKIFRI
                     KKLDSCRTQLLNSNVLLMAEDLGDVIDSGVMKLRADFYIEVPEEVGRIMKLYRNYSRM
                     NDDDRSLIKEIRGSGFTKGHFYRGVD"
     gene            complement(12601..13158)
                     /gene="zapA"
                     /locus_tag="PKI50_07300"
     CDS             complement(12601..13158)
                     /gene="zapA"
                     /locus_tag="PKI50_07300"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNT03544.1"
                     /translation="MAEKNKTTVSIFGTEYVMVAEKSEEYIYDLAAKVDEAMKEIARS
                     NSRYNPTMVAVLTALNLADELQKSQDELAETAEKLENIQGEMQRPFEELNEMRQELEA
                     IKEQYTKMQSEYTRSQIELGKISREWAKAQEELRDLRCELDVSRQTIEDVQNKLFENQ
                     IELLKTKKELDETKIKRIDKNRNVK"
     gene            complement(13311..15704)
                     /gene="pheT"
                     /locus_tag="PKI50_07305"
     CDS             complement(13311..15704)
                     /gene="pheT"
                     /locus_tag="PKI50_07305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNT03545.1"
                     /translation="MLVPVKWLKDYVDIKFDPKGFADAMTMSGSKVEGIEELGKEIEN
                     VVVGKILKAEKHPDADKLIVAQCDVGTGVIQIVTGADNIKEGDYIPIAKHGSTLPGGV
                     KIKKGKLRGVESNGMMCSAQELALDLDKVSENMLDGIYILDGEYPLGADIKDILGLND
                     TVVEFEITNNRPDCLSILGVARETAATFGVAMKYPDTGFKENSENIKDYIDIEVKNSE
                     LCPRYMARMVKNIVIKDSPAWMQERLIKAGVRPINNIVDITNFVMLELGHPMHAFDYR
                     DLEDKKIIVRNAAPGEKITTLDETERKLDESILVIADGKKPTGIAGVMGGYGSEIKND
                     TTAIIFESANFNPVNIRMTSKKLGLRTEASSRYEKGIDPELAEMALNRACSLVSRLGA
                     GEIVGGSIDIYPSPVKVRKLRLNIKKANDFIGVQDITKEMAVKYLTTLEFKAEIANGD
                     LEVTVPTFRDDVEGEADLIEEIARLYGYDKIPVKLMDTTFTQGGKNNRQVIRDLAKSN
                     MAAQGLYEVVTYTFVSPAVYNKINLKAENPLRNAIKLLNPLGEDQSIMRTTIIPNMLE
                     VVSRNYNMKVSEGRFFELSKVYLAEELTVEKLAEERETLTIGMYGDVDFFDLKGVIEN
                     LMDELHIDKYRILSSNNDSMHPGRTAELLINNRRIGCLGEVHPEVLDKYDIPVRVYIT
                     ELNFEEILRQCDMNIKYKALPKYPSVARDIAIVAAEEITAGQIEEIIRNKGGKLVEDV
                     KLFDIYRGSQIKEGYKSMAYSIVYRSDEKTLSEEDITKVHNKIINSLENQVGAELRQ"
     gene            complement(15735..16754)
                     /gene="pheS"
                     /locus_tag="PKI50_07310"
     CDS             complement(15735..16754)
                     /gene="pheS"
                     /locus_tag="PKI50_07310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNT03546.1"
                     /translation="MKETLEKIKLHSVKELESVQSQKELEEVRIKYLGKKGELTQVLR
                     GMGGLSPEERPVIGQLANEIRDYINEKIEETAYCLKRKDLEIRLGKEFIDVTMPSKRK
                     ALGNKHPLTKVQDEINQIFIGMGFTIVEGPEIEKSYYNFDALNHSKYHPARDMQDTFY
                     FTEEILLRTQTSPMQVRTMELQKPPIRVLVPGKVYRRDEIDATHSPMFHQNEGLVVDK
                     GITMANLKGTLDVFAKKLYGEDTKTRFRPHHFPFTEPSAEMDCTCSACKGAGCRVCKY
                     EGWIEILGAGMVHPNVLRNCGIDPEIYSGFAFGMGLDRIALLKYDVEDLRLFFENDKR
                     FLSQF"
     misc_binding    complement(16813..17039)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17195..17545
                     /locus_tag="PKI50_07315"
     CDS             17195..17545
                     /locus_tag="PKI50_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03547.1"
                     /translation="MLDRRKGPDKLVKWVKWSGIISWILVAIVLFITIIAKPSFESYM
                     DKSFHVKLQSNWDINLMQYVLVLLVLLFFFCIISIAINLSRCRRKTDRFNKTLILNAV
                     ASFIGILLYLLFFI"
     gene            complement(17528..18886)
                     /locus_tag="PKI50_07320"
     CDS             complement(17528..18886)
                     /locus_tag="PKI50_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT03548.1"
                     /translation="MKKHQFSILLAYILILLISSGCAEAFQYSIGCADIKTNQSFSFF
                     IVSDPHYISKETYDNGKAFQDFLASGDGKMLNHTDELLDALIKDIRVENPDFLIITGD
                     LTCNGTRQSHIELAEKLKTIEDIGTCVFVVPGNHDIQNPYARKYIGDEVTDIETITSE
                     DYVNLYDPFGYEGCVSRDPNSLSYLAMPAEDIWLLMLDSTDTDRNMARKYPEQSGYLR
                     PDTLKWIEQCAALAKENNAKLIAVMHHSLIDHSEMIKANYTIKNSKDALKVFRKHGIE
                     IVFTGHIHIQDIKSDEYQGNRIYDIGTSSLAVYPHQYGRMKFTPYVGFDYRTIPLDME
                     YYDIHENAKDGTPLNFEDYSASFFTEQCGRMHQECLKQLEGLSDAERETVLETVCRMN
                     MLYFAGYRNEALNDIVNTEGFKKLENIAPCFTKEYAMKMLDDERTNNNTLLIPISLNE
                     KQ"
     gene            complement(19037..19243)
                     /locus_tag="PKI50_07325"
     CDS             complement(19037..19243)
                     /locus_tag="PKI50_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03549.1"
                     /translation="MDKIKLTEDKKKGSGWGTRLLMITGIAVVLLSAMALYLMRPSPV
                     EQDAGLIDEEESEEDKVFDKAESG"
     gene            complement(19388..19464)
                     /locus_tag="PKI50_07330"
     tRNA            complement(19388..19464)
                     /locus_tag="PKI50_07330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19428..19430),aa:Val,seq:gac)
     gene            complement(19631..20206)
                     /locus_tag="PKI50_07335"
     CDS             complement(19631..20206)
                     /locus_tag="PKI50_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03550.1"
                     /translation="MANDKFFSVMEYDISLGTGGKECIKYVPNSNKISFIANDKLYIF
                     DRDKGEKTATLDEVDDQYSWDTNGEKILYADKDEKIYMYDMIKKEKQFICEGIHPVLS
                     NNNEYIAYMAKDHILVVREIKTKKEWRGIDLLRDQRFVFSPDDKYLALAKQEYSIANN
                     PYTIMYAVDYRKGKVTQLFKGDYSSYGFDWK"
     gene            complement(20374..21183)
                     /locus_tag="PKI50_07340"
     CDS             complement(20374..21183)
                     /locus_tag="PKI50_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT03551.1"
                     /translation="MGRYTREEIDGGYYHLIARGNNREYIFNRDIDKGYFLKLLNESR
                     QNMRFKLMGYVLMDNHYHLLVQTGNVKLQKIMHQINNKYSKYFNAIYERCGHVFQGRY
                     KASVIRNELYLLKVLAYIHQNPVKAGMVKKVEDYKWSSDKFYRRKSDGFIETKMIIEM
                     IEEGYRKSNKKYEDIMNEVEETDYDTLNEIGDRAYEIMMKSSINTEKRLSLEEIMTGE
                     VGCTEIINKIMNGSRESRYKEYKLKYARKAQENGYTLREIGLSINQSGVSI"
     gene            complement(21332..22303)
                     /locus_tag="PKI50_07345"
     CDS             complement(21332..22303)
                     /locus_tag="PKI50_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03552.1"
                     /translation="MGNLFPILLIGCLSMLTAEVFAGVSHVWYVELWDVLITLPLYTA
                     HTLFFFRIAWTRKKTSISQLYLFGMIFALYESWITKVLWAGYPDSAGPVLGTFLGIGI
                     SELPILVFFWHPIMSFIVPVLIFQVMTGKILDGHSRILRKTAKKTSVIIMFLIIVSTF
                     IVNGNKSDLLLSNLSVIGSIILIVVFYYLSRKTDLEVLLFRKAGFVIIIIYMFLLYVA
                     SFFLIVPERIPRSIIPYISIIVLYAVPIILIRKSEKEETKLTAQDKNRYNIKDLAIFA
                     AITIIAVNSAVILPDISSVVFVFSYYLLGLAGIVLFVSTVFGSIKPK"
     gene            22402..22721
                     /locus_tag="PKI50_07350"
                     /pseudo
     CDS             22402..22721
                     /locus_tag="PKI50_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044137.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22779..23021
                     /locus_tag="PKI50_07355"
     CDS             22779..23021
                     /locus_tag="PKI50_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03553.1"
                     /translation="MAEMDENDNGVDVCYNVQAVVDGKHKLIVDCDVINNPADHGQLS
                     EMSFVRRIFLRLMVLKLLLIKGTTMLMILRYARRKK"
     gene            complement(23048..23236)
                     /locus_tag="PKI50_07360"
     CDS             complement(23048..23236)
                     /locus_tag="PKI50_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03554.1"
                     /translation="MEGVFFLYNGHGDVVQTVCEEGEIENQYDYDIFGNPTLTVEEYS
                     NSIIFKFICIKPGFSCRV"
     gene            complement(24286..24690)
                     /locus_tag="PKI50_07365"
     CDS             complement(24286..24690)
                     /locus_tag="PKI50_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03555.1"
                     /translation="MLEHITLCHKDHEHDNPSYADVSVWINKLLAIEFKGRDILVKQM
                     ENSKYELINGEGFISIKFCVNDEKEKFPFRVRVPVEMIAFQQGRHPIMFLLHVLGGIV
                     DELEIYTADGSLIDSKISVLNVNHKVADLLKD"
     gene            complement(24662..28714)
                     /locus_tag="PKI50_07370"
     CDS             complement(24662..28714)
                     /locus_tag="PKI50_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT03556.1"
                     /translation="MSKRITNYTYYPNGLLRETIQSSGSNVKYHYDNNGNLIKTESLR
                     ENGIYDIAKYEYDSENRVKKAVQLVDEDSILTEGIDDVAELRDSEYPDKINIITGYEY
                     DLLGNRTKVISPKAYGCADEEAGAGYAVTYSYDILNRLERIGRKYNGSEVSVRYFYDE
                     AGNKVKEINEKDLETVYTYDSMNRIESMTDTLGKTYEYGYDPAGNKTYETNPKGDTIS
                     YSYDKLNRVIEVIDPYGKVINRKVYDEKGNIIKDIDAKGYLSGETDVERYGIEYEYDL
                     AGRIVKTIDPEGGQTKYEYSQYGELAKHTDALGNTSQYEYDNAGKLVKVTDALGIATK
                     YTWDKAGNKLTMTDGRGKITKYCYGSFGLLISYIDADEQETVYKYDLSLAVAAMKDRN
                     GNETIYSYDNRGLLTEKKVPETGDTVSYTYDVLGNRETMTDDTGTSIYAYNGNNWLIS
                     IEKDGELQISYAYDDIGNIASVTDKLGNATEYTYDRSSRMESVSFEGRTIEYEYDENG
                     NRKSIDYDGEVEEAYTYDKNNRLLELVNRKAGGGIISSYSYTYDKAGRQTSRTDSYGT
                     TEYTYDKAGRILKVEAPGKTTVYTYDGAGNRISQDETYTSEQILPAIDGTGLGEIKYI
                     VKRSEYVYGNTNRLLKLVEKLKNAGGMELLQKITSFRYDDNGNELRRTVEYISLYNKE
                     NPKVYEAAVYDEETTEPIHAVVDQTVSKYDGFNRLVRTEIIRGGVKTTVEYAYNGDGL
                     RTEKTVRRSNKGNAAETTSYLYDRQHVILEARGNDEIRYVRGINYIARIDNTDKLSYF
                     LYNGHGDVAQTVSEDGEIENQYDYDIFGNPTLTVENYQNSIRYAGEFYDAETGLYYLR
                     ARYYDPYIGRFISEDSYWGEDTNPLSLNLYTYCENDPINFIDPTGHSKIDDIIKEIDS
                     EKDKWWKEANSGRGGLGQKDWNQAQRDANKRANELRDELARLEKGNKEVEKLGKSKGD
                     DAGDWERYKYKKEVERSAKDGKVDREERKKIERRLEELTAAEAYGVNVPGVNRNRLKV
                     DFGVSSIDELVNVSKDSIRFINSSGNRDLWKRVGKDVGIAEIENRRHPSIHNQEVLSD
                     AKHNRGLGLTSEQVAFAALEDIHGVRDYLAPSKKASAGQNESRESLRNEVYEILTGKE
                     SRYHKNIAGQWTDINRPDSRNNFTEFLSGSIVSDADMLLNRYSTTNVDLFRMTDAVIK
                     SAAAIMSVRYILSEIAPVAEYYRAKFSVWKQGLNPSTSGFKPDLQLFGDNATQGEGSK
                     GYQTPNGGGGITNSIKTGNGTVTFGHGGRHVTGVDISSVEQTIANKVPKLGVGERYTG
                     TVNVNGINIEYRAYGVSEGVTNVGTYYPLP"
     gene            complement(29163..29765)
                     /locus_tag="PKI50_07375"
     CDS             complement(29163..29765)
                     /locus_tag="PKI50_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03557.1"
                     /translation="MWGSKILSKTEGNDKYLFDFKKQWVEGYAEVIKAAAKLYDLPEY
                     LIAGVVYSEVGGDPLWIDDVAYFVRDNIPKGMRNKIPGGKNPDLTSYGNVSIQVEVAT
                     RTLGYNLENLTDSQRRIIIKSIKDPVQNILISAKHLSELRDIDFKGKISSHLTIDDVK
                     VIATRYNRGPNLSIDKIRDNMSYGTFITKRKSLLGDMLNE"
BASE COUNT         7350 a         7397 c         5041 g        10054 t
ORIGIN      
        1 tcctgtcccg ctttcagcgg gacaggagaa acgtccccgt gtcccgaaac gtcctcgtgt
       61 ccttcgtgtc cttcaagctg atacttatta ataccccagt tcttttccta taataattac
      121 ctcaaattcg gtcttgtacg gccttctttc aataattgtg gtaacgctgc acattctctg
      181 cgggctgttg cagtcattgc atttctcggt atgcgcacat ggagtatcaa gctttagcct
      241 tcttgcattt ttatatgtat tctctttaat cctttttaaa gctgcatcca ggtcttttgt
      301 tatcttattg actccgcaca aaacaaatac ttttttgggt ccaaaaatca ttgaagcaac
      361 tctgttcccc aagccgtcaa tattaaccag ttttccgtcc tcggtcaggg catttgtacc
      421 cgacagatat acatcggctt tttgtgcttt gtcccttgca taatccattc cttcaggagt
      481 gctctccagc caatggaaat ataccctgtt gcccctttca ataagcttct ggggtatacc
      541 cagggagtat acggtcatgg atcctccgat tcctactgtt gctccatccc cgattttacc
      601 catcaaatag gatactgccg tatcggagtc atcaaaatat gatgccttca tattgtgctt
      661 ttccaaagct tctattgttt ttttaacaga ttcattcata ctatcacccc caagatatat
      721 tatatttcaa gcaatgcctg gaattcaagc actacaggtc gtaatacagc tcaaattctg
      781 ccggatgagg aattatgctt atcttcttat gctccttctc aatcttgttc tttatccaag
      841 tttcaattag gctctttgtg aacaccccgc ctttcagcag gaattcatga tcctgcctta
      901 gagcttccat agcctcctca aggctctttg gcaggccttt tatcttcttc ttctcctcat
      961 catcaagctc aaaaatattt acatcgtatg gtccgaagcc ctccttgaca gggtcaattt
     1021 tgtttattat accatccaga ccggccatca gcagtgctga ataagcaagg taaggattgc
     1081 aggtggcatc ggaagatctg aactcaaacc tcttgctgtc aggagtattg ccataacccg
     1141 gtattctaac gatagcgctt ctgtttgcag ttgcaaaaca tatgcttacc ggtgcctcat
     1201 aacctggaac caacctctta tatgagtttg tgctcgggct cgtaaaacct aacaatgcgg
     1261 gagaatgctt caaaagccct ccgataaaat acagtgctgt ctggctgagg ccggaatatc
     1321 cgtccttgtc atagaataaa ggctttccat ccttatacag ccacatatgc acatgcattc
     1381 cgcttcccgc ctcgccaaaa ataggcttag gcatgaaggt ggcgctcttg ccgttttgta
     1441 ttgctatgtt tttcaccaca tatttgagca aatatgtctt atccgccata tccttcagtt
     1501 tccccggctc tacttccagt tcgacctgcg ccggtcctcc cacctcatga tgatgatact
     1561 ttatgggcat gccgaactct tcaagcataa ctgtcatttt gcttctcaag tcatttgtca
     1621 gatcataggg aagcgccaga tgatatccgc ccttatgcct tactttgtag ccaaggtttt
     1681 tttcatactg gtcattagta ttccattcag cctgcgccga atctattgcc acctcctggt
     1741 gctccggcct gctttcgtaa gctatctggt cgaataaata aaattcaaac tccggtccga
     1801 ttatattctc atccgctata ccggtagagg ccatgaactt ctcagccttt tctgcgatat
     1861 atctgggatc cccttcatat cgccttatcc cgccctcaag tgcaaagata tctcctatca
     1921 tgctgagagt cggaatttca caaaatgggt caacaaaagc cgtattcaag tccggtataa
     1981 aaaccatgtc acttttttct acagtcttga aaccatagtt ggaaccgtca aatcctatac
     2041 cctcctcaag tactttctca ttcagcctgg tcaccggaat agagaggtga tgccatctcc
     2101 ctgctaaatc agtaattttg aagtctacta ttttaatgcc ctttgaatta caaaattcct
     2161 tgacctcggt gagattttta aacaatacaa taaccccctt gtaatattgt gctaatttta
     2221 gtatatttaa acatacggaa taagtcaaac aattaaaaaa cgcggcataa gccgcagtct
     2281 tctatttact ttcttctatt aaaggcttta gtacctcatc catcttgtcc ctgtttacca
     2341 caaagaaaga cagtttgttt atggtttctg ccctgccggg tattgtctgt atggtgatat
     2401 tctccttttt tatcttcttt gctttggtaa tatagtacat catctgtttt ggcggcatat
     2461 ttgtctttac atattccttg acaggctcct gaagcttaag aaagttagca gcaatattgc
     2521 ctctcattac tttatcaata cctaatctga ctatctcctg ctgcatcttc accctgccca
     2581 ggtctccctc cttatagccg ccgcccttgt tgttcttacg gtatctcaag agctttaata
     2641 tatcctcccc cttgctcact acctctcctt ttttaaaatg tatatgcaaa tcatcaaccg
     2701 gatcatcata gttcatatcc ataggtacgg ttaccttaat gccgcccatg gcatcaacca
     2761 gttctttgac agcctcataa tccacctctg caaaataatg cacatccagt cccgtaagag
     2821 atacgacgct ttcggccaca ttatccgccc ctccccttcc gtaggaagag ttaatcttgt
     2881 tatttgcaga gatcttatgt ccttttcttt caatatatgt gtccctgggt attgaaagca
     2941 catccagcgc tccatcctct gggtcgaagc ttgccagcat tattgtatcc gcaagaccct
     3001 ttgccacacc cataactaca acatttaccc ttttttccgg taccggatag ccttccgcac
     3061 cctcctccac gcttattttg cttaaaaagt tatatgtaaa gccatatatg gctgctgcaa
     3121 tcagaagtac cgcaaccaca acagctgtta ttatcttccc tttagcactc aatttttttc
     3181 ctccaaatcg tattagatat taatccatgt cacaagtaaa taataatgca gacacaactt
     3241 atagtttaat tctccttata taatatgtaa agtgcattta cagccaaaaa gtataccata
     3301 aaaacaatta agtgctttta tggtatactt tcctgccgtt ttgtacttct acatgataat
     3361 atcccgatac cttcagatca tttttgacgg caagcgccat gatattttct atttcatcag
     3421 ctttcagttt tgctgaaatt ccgcagcttg cggttatttc ccggggaacc ggcatgatgc
     3481 ttatgttaaa accccagtcc cttactgcct tttcaaactt caaagcatag tgggtagaat
     3541 caaaaacggc aacgtagtat tcattcattg cattcactcc tcattatgtg cttaacagtt
     3601 acaaggattc accaaaaagc tcatatatgc ttccccgaag ttaatgaact cattgctttc
     3661 catcattgac aatatttcat ccatacaggc atatttcact tcactgactt cttccttctg
     3721 tatcctgaca tcttccggat tgatatcccg gcaaacaaac cataaatctg aaaaaacact
     3781 tttatacctg accctgtata atctcttcag agcgtccggt tcaaagctta gccccagctc
     3841 ttccttaact tctctcaatg cggcatccag actgctttcg ccgctgattg ccgaacctcc
     3901 ggctgcgccc cagactccgg gccaagcacc ggcctgtgag gacctttttt gtataaggta
     3961 ctcacctttg ctgtttctta tccaaatatg aactattaaa tgatattctc ccttttttaa
     4021 aggcttgcct ctttcaatag ttcttccggt caggcggccg tcttcatcca atatgtccca
     4081 taactcaaca ttcttattca taaaactgcc tcatcctata cattttagga taaacatcag
     4141 ctaattatat ttcggatatc ctaaatgttt tccttgattc atagaatacg ccaaaggttc
     4201 tgtgccattt ttaataagct gtttaccggc gtattctata taataaggtt tttatcgaag
     4261 ccctcaattc ggctatgggt atctgccccg gagcagaagt cttctcaacg gctccgaaag
     4321 tcatatctga cccgaacata cctccagcta ttctgctgat aatgccaaga gcacccattg
     4381 atactgttat gaggggtgtg taaggcaact cgatacgggc tttcaatgta gccgaaagca
     4441 gttttaacac atcttcctca ttcttgggca ttaccgcaat ttttgctatg tccgctccca
     4501 aggcaacttc ttcccttatc ttattcaata aaaactcaac agaaggcgtt tcatcaaaat
     4561 tatggtaaga tatgataagc ggtatatcat ggtcatatgc ttcttttctt atttcttcaa
     4621 ctgcattttt gcggcttgca agttctatat ctgcggcatc aacctctcct gtacataata
     4681 ccagcctgat gatttcatac cgtatatttt cttcaacctc aatgaacccg ccttcaaagc
     4741 tgcccctgaa tgtaaaaata agaggtattt ccccgattat gcttctgagc atattaagcg
     4801 ctttcattac ttttataggg ctgcaggcat ccttaaaata atccgcccgc cattctatta
     4861 tatcagggca tagctggatc gtattttttg ctgcccgctc caatccgctc tcctcttctt
     4921 ccaatattgg tatacaaatc agcatatcag gcccgccaat ggtcctattc ccaattttta
     4981 tcggtttttt tgcctgtaat ctcataacaa atctcctgtt ccaagcccaa aaacatttca
     5041 ggcttcatac ttattcaaaa tctttttctg tcattaccac gattttatta tccagcagca
     5101 gctgtgcggt cacaccgttt ccctctttaa gctttcctga aaaagtcccg tcatatatta
     5161 ttccgcagcc gcaggagggg cttcttgact taagcaccgc cagccggcag ttatgctgct
     5221 tggctaaagc aagggtttct ccggcaccct taataaaatc tgccgttaca tcttctccta
     5281 ttttgtttat gacccttgat tttccttcca gtacttcctt tccggaaccc tcttgtatct
     5341 cgcagggctc ccttggtgtc agcaagcctc ccagttcttc gggacataca ggtatgatat
     5401 tgttctccct tttaagctcc agcagcttgg gagtaaaatt gttccccccg ttgtatttgc
     5461 agtttgtacc caataaacat gcactaacca gaatattcat ccaatcactt ccatcatttt
     5521 atttccacta tggtagcccc aatgccgcct tcaccaaacc cgccgcttct gtatgacttc
     5581 acatggctgt ggcttttcag aagctgcatt atgcccgccc ttagaacccc cgtgcccttg
     5641 ccgtgaatta ttgtgacctg gggcaggttg cccaggtatg cgtcatccag gtatttatcc
     5701 acattcatca gggcttcatc caatgtctgg ccccttaaat ctatctgtga agcaacagcc
     5761 attgccttgg cggaattcac cttgacaccg gaactcctct tgggtttttc cttctcttct
     5821 tccgctgctg tcagattgtt tacatttaca tttattttca ttatacctgc ctgcacggtc
     5881 agattcccgt cctcatcagg aagtgtaaga acacttccct tccggtcaag gttgactatc
     5941 ttcacggtca tgcctatctt tatgtccttt aacggtgcgc tgccggtttt tttgaacaat
     6001 gattctccaa tcttatcttc tatttcattt tcccgttgtt ttagcttcat cctcgatttt
     6061 tcaatcattg cagcatccct aagttcccgg ataagctcct ccgcttcctc ctttgacctt
     6121 cgcactattt caagggcctc actgcgagcc tcccttacaa ttctgtcctt ttgagcttca
     6181 agcttttcaa gccttctgtc catttccgac ttttcttttt ccagctcaaa tttcagtctg
     6241 gaagcaacat cccgttcttc ttcagccttc cttttatcct cttcaaggct ttttatcaaa
     6301 tcttcaaatt ttacatcatc ctgagaaatg aattcctttg ccctttttat tatttcctcg
     6361 gaaagcccaa gctttttgga tatttcaaag gcatttgact ttcccggtat gccgattaag
     6421 agcctgtacg tcggcctcaa agtagccacg tcgaattcca cgctggcatt gcatattcct
     6481 tcggtagtaa gggcatatat cttcagttca ctataatgtg tagtggcagc agtccttata
     6541 ttctttttat gcagcagttc cagtatggac attgccagtg ccgcaccctc cgttggatcc
     6601 gtacccgcac ccagttcgtc aaacagcacc agggaatttg gggtgacatg ctcaagaatg
     6661 gacactatat tggtcatatg ggaagaaaaa gtactgaggc tctgttctat gctctgctca
     6721 tccccgatat ccgcaaacac ctcatcaaaa actgccactt cactgtagtc atttgccgga
     6781 atgtgaagac ctgactgggc cataagcgtc agaagccctg tagtctttaa tgtgacagtc
     6841 ttcccccctg tattcggccc tgtaataacc agggtattga aggtttctcc cagataaatg
     6901 tctatgggga ctacggactc agcctttaac aaaggatgcc ttgctttctt gatgttcact
     6961 atgcccttat tgttgaccct tggctcggtg cacctgaaat ccagagaaaa cctggctttt
     7021 gcaaatatga aatccaatgt cgccaatatc tccatattgg tcttgatgtt gtcatacttc
     7081 tcatcaacaa gtgccgtaag ctctctcagt atcctttcta tttcattctc ctcatccagc
     7141 ttcagctttt ttacatcatt attcatctct acaatcgcca taggttcaat aaaaagagtt
     7201 gcaccgctgg ctgactggtc atggactatg ccgggtatat ttgctctgaa ttcctgtttt
     7261 accggtatga catggcggtc tcctcttaca gttatgatcg gttcctgaag atacttctgg
     7321 taagccggag atgaaatcat atggttcagc ttttctctca ttgaagcagt tttttctttt
     7381 atttttctcc ttatggtgct aagctgaggg ctggcgttgt ccgctatttc ctcttcgctg
     7441 attatggcgc tgtttattct gtcttctata cctttgaatg ctgacagggt atctatataa
     7501 tactcaatta tcggaaagct ttcctcacct ctgtcctcat tcatgaaagc tttcaacctt
     7561 ctggcagctc ttaatgtgtc tgccacctta agaagctctc ccggttccag agccgacccc
     7621 atcttgacct ttttcagggg cattcggatg tcctgtactc cgccaatggg gatgctgcct
     7681 ttctttagtg taagatttac agcttctgtg gtttcctgaa gcagctcctt tatctcattg
     7741 aggtcaccgg agggcttcaa atcagccgca atatcccttc ccataggtga aacggtattt
     7801 tcgcacaaca tatctataat cttattatat tcaagaattc tcagcgcctt atccttcaat
     7861 ccattcacct cttcacgcaa tccgcatccc gcagcccgta ccttatttta catctcctta
     7921 tgggctggga ggtagaaaca gaattccgag ccttctccgt tcacgctttt tacatttatc
     7981 tcacagccgt gagcatttat tatgttcttt actattgcca gcccaaggcc ggtgctgttc
     8041 tccgatcttt tcttgtcaac tatataaaat ctgtcccaaa tgaagggcag atccacctca
     8101 ggtatccctt ccccaaaatc ccttacgcat acatatatgt tttcaccttg cctgtatgcg
     8161 cttatcaata tttcaccatt atcatacgaa tgcttgatcg cgttgtaaat caaattaatc
     8221 agcacctgtt ttattctgtt ttcatccccg aagcagttaa gtgcatcctc cggcgttttc
     8281 gtgaatttaa tgcctatatt cctttttaat gcatggggca gcatcttgtc tatcatgctc
     8341 ctgatcattc cgtccatgct gaagggcata gattgaagct gtacgtatcc cgcttccatc
     8401 tgcgacaatt ggagtatttc atccaccatc cgctttaacc ttgctgtttc atccattatt
     8461 ataccaagat atttatttct ttcctcctct gattcaacaa gcccgtcctg aaggacctcc
     8521 gaatacccct gcaggtagct taacggtgtc cttatttcat gggatacgtt ggctatgaac
     8581 tctcttctta tctgttcaac acgggtaagc tgctttgcca ggttgtttat agtatcccca
     8641 agttccttta tctccctgct ggaacgcagg tcaatttttg tattgtaatt gccctttgat
     8701 atttccagtg cggcattgtt gataagcttc agcggccttg aaaagctttg tgaaagaaaa
     8761 tatgatattg cggaagcaag gactattgcc gtgataaata tatataaaaa ctgcttcctt
     8821 atggcatcaa ttgttgtctg catatactcc agggtggtga ttatatatac ggctcctgta
     8881 atttcgtgct gctttaatgg tccgccctcg tagttttcac tgatacgctg cctgttctcc
     8941 tgggtcaaaa ccacattttt tttaactgga acgccaacca gaagtgtatc catttccttt
     9001 ggccataccg aatgtgggtt gctgatttct ttgctctgct cgtagactgg ttccccttta
     9061 agtatcctgc caatatgatt cttcccaaaa atactatcca gtttgtattt gtttgtacct
     9121 tccacataga ttatttttcc gtcattgccg gtgattatta ttatatcatt tactcttctc
     9181 attacactgt agtaaggtat ggtgaaatgc tgatccctgg ctacataatt tgccagcctg
     9241 aaacaatcct tctccaactg ttctttctct aattcgaaat aaaaatcatt taagaattcc
     9301 gtctggaata tcagggtaaa aaccaggagc gccaatgtca ttgcaattat tgatgcccat
     9361 agcttgaatg ctattccgta gcttctcatt tcggcacctc aaacttatac ccgacacccc
     9421 acatggtact gatacagctg ctgtaatctc ccagcttctc tctcagcttc tttatatggg
     9481 tatccacagt tcttgggtcg ccgtactgct catagcccca gaccttaaga agcagctgct
     9541 cccttgtaaa gacgattttg ggattggcag ccagaaaata caataattca tattctttat
     9601 tggtaaggtt gacaggcctt ccgtttaccg ttacttccct ggacagcctg tcaatcacaa
     9661 tatccccaaa cctataatat gaaaccgctt cattactctt aatttctgcc cttcgcagca
     9721 aagctttaac tcttgccatc agctctttcg ggctgaatgg ctttaccaaa tagtcatcgg
     9781 cacccagctc gaagccgaag agcttgtcaa attcctcccc ccgtgctgtc aatagtatta
     9841 tgggaatatt cgatgtcctt cttatttccc tgcatacagt ccagccatcg atttcaggaa
     9901 gcatgatatc aagtataaca atgtccacac tgttcgttct gaaaagattc aatccctgtt
     9961 tcccgtcaac ggcctcaata atcacgaagt cctcttttct gaaaactatc ttgattaatt
    10021 ccctcatctt ttcttcatct tcaataagca gtaccctaac actgttattc acccgcagtt
    10081 cctcctttca atcgacaccc ctgtaaaaat gtcctttcgt gaatccggat cccctgattt
    10141 cttttattag tgacctgtca tcatcattca tcctgctata gttcctgtac agtttcataa
    10201 ttctgccaac ctcttccggc acttctatat aaaaatcggc cctcagcttc attactccgc
    10261 tgtctattac atctcccaaa tcttcagcca taagcagtac attagagttc agaagctgcg
    10321 tcctgcatga atcaagcttt tttatcctaa aaatttttcc tttctcgtct attataccat
    10381 aattggagct attgcatccc cgaggataat tgcagttgtc tctgaacatc gaaaccgggc
    10441 agtattcggt ggtcataacc ggcaaatatc catacacaac agactccgcg gcagctgcgg
    10501 tatttgaggt tattgcttta atctgcggga gattcaattc cgcggataat ccgacggctt
    10561 ccgctccttc cctgtgccaa tggcttgcgg atacggaatt aaacacattc atgctgtaat
    10621 tgactcttac cctgcggata cctgaatttc ttgccagttt caactgtccg gcatccgata
    10681 ccactacgcc gaaatatctc atatccgctg cctttattat ttcctcaagc cgctccttta
    10741 tttttcctgt ttccctgtcc gtcataatgt tcggaagcac ataaaacgcg gtcttgccca
    10801 tacttctgca tgtttctgcg atttttacgg tttcggaaac aatatccccc tcatatacat
    10861 cactgagaat atataccgaa tcagctcccg aatttaaagc acttacagca gaggcatagt
    10921 ttcttatccc tgctatatat tccaatgaat cttctccgga ggcctttacc ggtgatatca
    10981 aaccgttgat aagctctgcg gcattaattt gaataatcgg gcggtcatat ttctgtgagc
    11041 gtatccgtga cagcagttcc agcacttttc ttctcatccg gtttatctgc cttgcaggta
    11101 tgtagcaatc cccgtctatt attgtctcag ttacatctat aatgtaaggg gtatcccctg
    11161 tttttccaag atactccaat attttttcaa tcggagtggc ggccttcgca gcagcctcgg
    11221 ccctttcctc atcggtatag cttacagtgt tgccgtcatc gtcatttact gttattaccg
    11281 ggtattctcc ggttttaact gttagactgc accggacagg gatcttcctt gtattctcag
    11341 gtgaatactc ctctctcgcc cttctgctca actcgctgtc aaaggttttg tttacccggg
    11401 aaccggcttc aaccctgtct cttatgaaaa cctccgcaat atctcctttt actgcggctt
    11461 ttacattcct gcccatgacc cttatgccgc tgattattgc accggtgctt ccgccgcttt
    11521 ttgatattat ttcgattccg tccccgtcat gcaatgaatt ctcttcaatt aaaagggata
    11581 ctatttcacc tttctgcctt attgcctttc ccagccttac acccctgttg tcgggcctgt
    11641 ctatgctgac cacatctctg tttcttctgt tgaacaggta tccttcggta aactccctgt
    11701 tgaatgcact tttcagttca taagcggcat cggcggtatc gaatgcttcg ccttttatta
    11761 tattatccag ggcattcctg taatgtctga ccactatagc cacatactcg ggttttttca
    11821 ttctcccttc tattttcagg gaagccactc ctgactcagt cagctccttc agcctgttgt
    11881 atgtattaag atccctggta ctcagcaaat gcttgtgccg gttctcaatc accgcttttc
    11941 cggtattgaa attatacaag gaatatttct ttctgcaggg ctgggcgcat ctccccctgt
    12001 ttccgctcct tccgcctata aggctgctca taaggcactg ccccgaatag ctcacacaca
    12061 aagctccgtg tatgaatatc tccagctccg catctgtgct ttcggcaatg cccctgacct
    12121 cttcaagggt caattccctt gccagcacta tccttttggc acccaattcc gaatagtacc
    12181 tggctccttc cgcattgtgc accgtcatct gagtgctgca gtgaaccggc agatccggaa
    12241 aggcattccg cgccagcttt aggagagcaa tatcctggac aattataccg tcaacaccta
    12301 tttgatataa gaagtttaca tagtccagag cttccgctaa ttccctgtcg gacagcagaa
    12361 tattaacggt gacgaatacc ctgacgcccc ttatatgggc gtaattaacc gcctttgtaa
    12421 ggctttggcc gtcaaagttg tcggcatacg ccctggcatt gaatttattt ccgcccaaat
    12481 aaacagcatc agcaccactt tcaacagcag cagctaatgc ttcaaaattc ccggcaggag
    12541 ccagaagttc tgttctgttc attatcatca cttcccgtgt ctcgtgtcct gtaattattt
    12601 ttactttaca ttcctgtttt tatctattct ttttattttc gtttcatcca gttctttttt
    12661 tgtcttcagc agctctatct ggttttcaaa aagcttgttt tgaacatctt caattgtttg
    12721 cctggaaacg tccagttcgc accttaaatc cctaagctcc tcctgtgctt ttgcccactc
    12781 cctgcttatc tttcccagtt caatctgtga ccttgtatac tcactctgca tttttgtata
    12841 ctgctcttta atggcctcca actcctggcg catttcattc agttcctcaa agggcctttg
    12901 catttctccc tgtatgttct ccagcttctc agcggtctcg gcaagctcat cctgggattt
    12961 ttgcagttcg tctgccagat tcagggcagt aagcacagca accatagtgg gattatacct
    13021 tgaattgctc cttgctattt ccttcatcgc ttcgtcaacc ttagctgcaa gatcatatat
    13081 atattcttct gacttttcag caaccatcac atattcggta ccgaatatgc ttaccgttgt
    13141 cttatttttc tcagccatat atatcaactc ctttgggatt aaaaactata tctaaatatt
    13201 attcttcaaa atgcgcggat tttccttcat tttttttaca ttaaggcaaa ataattgtga
    13261 aacagcatat caactcacac tgtataccgc tgatacgctg tcccattatt ctactgtctt
    13321 aattccgctc ctacctgatt ttcaagggag ttaattattt tgttgtgaac ttttgttata
    13381 tcctcttccg aaagagtctt ttcatcggat ctgtatacta tggagtaagc catactctta
    13441 tagccttcct ttatctggct tcccctgtat atgtcaaata attttacgtc ttcaacaagc
    13501 ttgccgcctt tattcctgat tatctcctct atttgtcccg ccgtaatctc ttcagccgca
    13561 acgattgcta tatcccttgc aactgacggg tacttcggaa gtgctttata ctttatattc
    13621 atatcacact gcctcaatat ctcttcaaaa ttaagctcag taatataaac ccttaccggt
    13681 atatcatact tgtcaagcac ttcaggatgc acttctccca ggcagcctat cctccggtta
    13741 tttatcagaa gctctgcagt ccttcccgga tgcatgctgt cattatttga ggacaatatc
    13801 ctgtatttgt ctatatgcaa ttcatccatc agattttcaa tgacaccctt caggtcaaag
    13861 aaatccacat ctccgtacat gcctatggta agagtctctc tttcctccgc aagcttttcc
    13921 acggtcagct cctccgccag gtacaccttg gaaagctcaa agaaccgccc ctcacttact
    13981 ttcatattgt agttccttga tactacctcc agcatgttgg gtattattgt agtcctcatt
    14041 atactctggt cctctccaag aggattcaac agctttattg catttctaag gggattttct
    14101 gctttgaggt ttattttgtt gtataccgcc gggctgacaa aggtataagt cactacctca
    14161 taaagcccct gggcagccat gttggacttt gcaagatccc ttattacctg cctgttgttc
    14221 tttccgccct gtgtaaatgt agtgtccatc agttttacag gaattttatc atagccgtaa
    14281 agccttgcaa tttcttctat caaatcggct tcaccctcaa catcgtctct gaaggttggt
    14341 accgttacct ccagatcccc gttggctatc tctgccttga attccagagt tgttaaatac
    14401 ttgaccgcca tttccttcgt tatatcctga accccgatga aatcattcgc cttctttata
    14461 ttcagcctca attttctgac cttcacagga gaaggataga tatcgatgct tccgcctaca
    14521 atctcaccgg cacctaaccg gctaacaagg ctgcatgccc tgttcagcgc catttcggca
    14581 agctccgggt caattccctt ttcatatctt gaggaagctt cagtcctaag ccccagcttt
    14641 ttggaagtca ttcttatatt cacagggtta aagttcgcac tttcaaatat aattgctgtt
    14701 gtgtcatttt ttatttcact gccgtaacct cccataactc ccgcaatacc ggtaggtttt
    14761 tttccgtcgg ctataacaag tattgactca tccagcttcc gctccgtttc gtcaagggtt
    14821 gttattttct ctccgggcgc tgcatttctt actatgattt ttttgtcctc caaatcccta
    14881 tagtcaaagg catgcatggg atggcccagt tccagcataa caaaattggt tatatcaact
    14941 atgttgttta taggcctgac gcctgctttt ataagccttt cctgcatcca tgcaggagaa
    15001 tccttaatca ctatattctt aaccattctg gccatgtatc ttgggcaaag ctcgctgttt
    15061 tttacttcaa tatcaatata atcctttata ttttcactat tttccttaaa accggtatcc
    15121 ggatatttca tagcaactcc aaaggttgct gcagtttccc tggctacacc caaaatactt
    15181 aaacagtcag gcctgttgtt cgttatttca aattcaacta cagtatcatt aagtccgaga
    15241 atatccttta tatccgcacc aagagggtat tccccatcca atatatatat tccgtccagc
    15301 atattttcag acactttgtc aagatccaag gccagctcct gagcagagca catcattccg
    15361 ttcgattcta ctcccctgag ctttcccttc ttgattttta ccccgccggg aagtgttgaa
    15421 ccatgtttgg caatgggtat atagtcccct tcctttatat tatccgcacc tgtgacaatc
    15481 tgtatcaccc cggttccaac atcacattgg gctacaatca gcttatctgc gtccggatgc
    15541 ttttctgcct ttaaaatctt tcccacgacg acattttcaa tttcctttcc cagctcctct
    15601 attccttcaa cctttgaccc cgacatggtc attgcatcgg caaagccctt cgggtcaaac
    15661 ttgatatcca cataatcctt taaccattta acaggtacaa gcatgtttaa tctcctttct
    15721 tccttattac ttacttaaaa ctgcgacagg aacctcttgt cattctcaaa gaaaagcctg
    15781 aggtcctcca catcgtattt cagcagcgca atcctgtcaa ggcccatgcc gaaggcaaag
    15841 cctgaatata tctccgggtc aatgccgcag ttcctgagca cattggggtg taccataccg
    15901 gcgcccagta tctcaatcca tccttcatat ttgcataccc tgcagcctgc acccttgcat
    15961 gcggagcagg tacaatccat ttccgcgctt ggctcagtga aggggaagtg atggggtctg
    16021 aaccttgttt tggtgtcctc tccgtacagc ttctttgcaa agacatccag ggttcccttc
    16081 aaattcgcca tagtgattcc cttatctaca accaaaccct cattctgatg gaacataggc
    16141 gaatgtgtcg catctatttc atctctcctg tataccttcc ccgggaccag cacccttatc
    16201 ggaggcttct gcagttccat agtcctgacc tgcataggag aggtctgggt ccttaacagt
    16261 atttcttcgg taaaatagaa ggtgtcctgc atatcccttg caggatgata cttgctgtga
    16321 tttaacgcat caaagttata atatgatttt tctatctccg gcccttccac tattgtaaat
    16381 cccatgccta tgaatatctg attgatttca tcctgcacct tggtcagggg atgcttgttt
    16441 cccagggctt ttcttttgga tggcatggta acgtcaatga attcttttcc cagccttatt
    16501 tccaagtcct tccttttaag gcagtatgca gtttcttcaa tcttttcatt gatataatcc
    16561 cttatttcat tggccaactg cccaattaca ggcctttcct cagggctgag tccccccatt
    16621 cccctcagta cctgggtaag ttctcctttt ttgccaagat actttattct cacttcttcc
    16681 agttccttct gggactgaac gctttccagc tccttgacag aatgcaattt gatcttttca
    16741 agtgtctctt tcatttttca tgctcctttc ctatatgcta tgttttacaa aaattaaaac
    16801 cttcatcccc aaaaaaggga cgaaggttat ccgcggtacc accctgcttg tttgcattcc
    16861 gcaaaccgct ccgaatataa cggtatttac cgtcaaagcc tactggtgct gttaacacca
    16921 tcttcagccc tgaatctccg gagtgaactt caactgcttc ggtgccggtg cgctctcagc
    16981 cggtggcagc acctctcttc aggccttccg caatttactc tctccatcaa cgattgcttc
    17041 actattatct ttttgatgaa tcttaataga ttatatcata agccttcaca ggttacaaca
    17101 aataaaacta tatagtatat aatatgcaat atattcattg attctcacaa tatagttgat
    17161 tataatttaa ttaaagcata gtgggggtga ggtcatgctt gacaggagaa agggtcccga
    17221 taagcttgta aaatgggtga aatggtcagg aataatatcc tggatcctgg ttgcgatagt
    17281 gctgtttatt actataattg caaagcccag cttcgaatcc tacatggaca agtcctttca
    17341 cgtaaaacta caaagcaatt gggacattaa tctgatgcaa tatgtcctgg tgctgctggt
    17401 gctgcttttc ttcttctgta taatatccat tgcaataaac ctttccaggt gcaggcgaaa
    17461 aaccgacagg ttcaacaaaa ctctcatact gaacgctgtt gcctctttca taggaattct
    17521 gctttatcta ttgtttttca tttaaagaaa taggaatgag caatgtatta ttgttggtcc
    17581 tctcatcatc cagcatcttc atggcatatt cctttgtaaa gcagggggct atgttctcaa
    17641 gctttttaaa gccctcggta ttcactatgt cattgagtgc ttcattccta taccctgcaa
    17701 aatacaacat attcattctg cataccgtct ccagcactgt ttccctttcc gcatcagaca
    17761 gtccctcaag ctgtttaagg cattcctgat gcatcctgcc gcactgttcg gtgaaaaagc
    17821 tggctgaata atcttcgaaa tttagaggag ttccgtcttt cgcattctca tgtatgtcgt
    17881 aatactccat atccaatggg attgtacggt aatcaaagcc aacataggga gtaaatttca
    17941 tccggccgta ctgatgggga taaactgcca ggctgctggt tccgatatca tagattctat
    18001 ttccctggta ttcatcggat ttaatatcct gtatgtgaat atggccggta aagactattt
    18061 ctattccatg cttgcggaac actttcaaag catccttgct gttttttata gtataatttg
    18121 cctttatcat ttcactgtgg tcaatcaggc tgtgatgcat aactgctatc agcttcgcat
    18181 tattctcctt cgccagggcg gcgcactgct caatccattt aagagtatcc ggccgcagat
    18241 atccgctttg ctcagggtac tttctcgcca tgttcctgtc agtatctgtg gaatccagca
    18301 taagcagcca aatatcttca gcaggcatcg caagatagct taaagaattg ggatcccttg
    18361 acacgcaacc ctcgtagccg aaggggtcat aaagattcac ataatcctct gaggtaattg
    18421 tttcgatgtc tgttacctca tctccgatgt attttctggc gtaaggattc tgtatatcat
    18481 gattgcccgg tacaacaaat acgcaggtgc ctatgtcctc aatagttttc agcttttctg
    18541 ccagttcaat atgactctgc ctggttccat tgcatgtcag gtctcctgtt ataataagaa
    18601 aatcggggtt ctccacccta atatccttaa taagcgcatc aagaagctca tcagtatgat
    18661 tcagcatttt gccgtccccc gaagccaaaa aatcttgaaa ggccttgccg ttgtcatagg
    18721 tttcctttga aatataatga ggatctgata cgataaaaaa gctgaaagac tgattggtct
    18781 tgatatcggc gcagccaata gaatactgaa atgcttcggc acatcccgag gatatgagaa
    18841 gtatcagaat atatgcaagc aaaattgaaa attgatgctt cttcatggcc aaccctccct
    18901 ttctttccga aaggagtgca acgggttaca ggtttacttt gttattatta tatcatactt
    18961 ttattaatat ttgtttattt ttaaacaatt attattattg ttattttata gtaaaaatac
    19021 cgcttttcac ttcaaactat ccgctctcag ccttatcaaa taccttatcc tcttcacttt
    19081 cctcttcgtc tataagccct gcatcttgtt ccaccggaga cggccgcatc agatataagg
    19141 ccatggcgga cagcagcacc accgctatgc ctgttatcat tagcagccgc gtaccccagc
    19201 cggagccttt ctttttatct tcagtgagtt tgattttgtc catacttcgc aggcctccta
    19261 tcatggtgcc tggtttttac atttcagcct ttcccatcat tttacgtttc tcatcagatt
    19321 cctgcttcat caaaaaacta aaaaagacta tacgcttaca ccttacatat agtctttaca
    19381 ttcattatgg tgggaccggc tgggctcgaa ccagtgaccc ccacgatgtc aacgtggtac
    19441 tctaaccagc tgagctacgg tcccatttac tttcatatta tatcatttat gatttgcttg
    19501 ttcaagaagt taatgatgcc agttcgtata attttgttct tataagctgt acgtggcata
    19561 tggtttcaac aggctggcac ctaacctact ctgaccttgc tcgtcagcat tgcctttcac
    19621 ctattcttct ctatttccaa tcgaacccat aagatgaata atctccttta aatagctgag
    19681 ttacttttcc ttttctataa tcaactgcat acattattgt ataaggatta tttgctatgc
    19741 tatattcctg ttttgctaat gcaagatact tatcatcagg tgaaaaaaca aacctttgat
    19801 cgcgcaataa atcaatgcct cgccactcct ttttagtttt aatttcacgc acaactagta
    19861 tgtgatcttt agccatataa gctatatact cattattatt ggaaagaaca gggtgtattc
    19921 cttcacatat aaactgcttt tcttttttaa tcatatcgta catatatatt ttttcgtcct
    19981 tatctgcgta taatatcttt tctccattag tgtcccacga gtattggtca tcaacttcat
    20041 ctagtgtggc agtcttttct cctttgtccc tatcgaaaat atacaattta tcatttgcaa
    20101 tgaaacttat tttatttgaa ttgggtacat acttaataca ctccttgcca ccagtgccta
    20161 gagagatatc gtattccata acgctaaaaa acttatcatt agccatgtcg tactcgaaaa
    20221 taactgtata atctcctttg ttctcatcta tcgatttata gcccagtatt ttagtcttgt
    20281 cagcattaaa acttaattcg ttgttgaaat ttcctttcag tgtgaatagt gccagtctgt
    20341 taacttgtta acttatttac cgtttagcag ctttcaaatc gatactcctg actgatttat
    20401 gcttaaaccg atttctctta gagtatatcc attttcctgt gcttttcttg catactttaa
    20461 cttgtattcc ttgtacctgc tttctctgct tccattcatt attttgttta ttatttctgt
    20521 gcaccccact tctcctgtca ttatctcctc caaacttagt ctcttttctg tatttatgct
    20581 gcttttcatc attatttcgt atgctctatc ccctatttca ttcagcgtat cataatcagt
    20641 ctcttctact tcattcataa tgtcctcata ttttttattg cttttcctgt atccttcctc
    20701 aatcatttca attatcatct ttgtttctat aaagccatcg gattttctcc tatagaactt
    20761 atcactgctc cacttataat cttcaacctt ttttaccata cctgctttaa ccggattttg
    20821 atgtatatat gccaatacct ttaacaagta tagctcattc cgaataacag aagccttgta
    20881 cctcccctgg aatacatgtc cgcatctttc atatatggca ttgaagtatt tactatattt
    20941 attgttgatc tggtgcatta ttttctggag tttcacgtta cctgtctgga ccaataaatg
    21001 ataatgattg tccatcagta catagcccat aagcttgaat ctcatgttct gccggctttc
    21061 attcaatagc ttaagaaaat aacctttatc tatgtctctg ttgaatatgt attcacggtt
    21121 attgccccgg gcaattagat gatagtatcc accgtcaatt tcttctcttg tatacctgcc
    21181 catcctcaat cacattccgg tattttcatt caacctatgt ttccttattt taccacaatt
    21241 tgtctcaaaa taaaagaaga aacccaagtt tgactcaggt ttcactcaaa tttgtcaatt
    21301 aagttaacaa gttaacaggc tggcacttag cctatttcgg ttttatactt ccaaatactg
    21361 tcgatacaaa taatactatt cctgccaacc ccaacaaata atatgaaaac acaaatacta
    21421 ccgagcttat atctggcagg attacagctg aattaactgc aataattgtt attgctgcaa
    21481 atatggcaag gtctttgata ttataccgat tcttgtcctg agctgtcagc ttagtctctt
    21541 ccttttccga tttccgaatt aggattatcg gcacagcata aagcactatt attgatatat
    21601 acggtatgat tgaccgaggt attctttccg gcacaattag gaaaaaagaa gcaacatata
    21661 aaagaaacat gtaaattatt atgataacaa aaccggcctt tcgaaataaa aggacttcca
    21721 aatctgtttt tcttgacaga taataaaata ctacaatgag aataatagat cctatcacag
    21781 acaaattgga tagcaagaga tcggatttat ttccgttaac tataaaagta ctgacgatta
    21841 ttaaaaacat tattataaca gaggtctttt tagcggtttt tcttaatatt cggctatgcc
    21901 cgtcaagaat tttccctgtc attacctgaa atattagaac agggacaata aatgacatta
    21961 tggggtgcca gaagaatacc aaaattggta attcacttat gccaatccct aaaaaagttc
    22021 ccaaaacagg accggcagaa tcaggataac ctgcccataa cacttttgtt atccacgatt
    22081 catataatgc aaaaatcatc ccgaacaaat ataactgtga aatagaggtc tttttccgtg
    22141 tccatgcaat ccggaaaaag aacagagtgt gggctgtgta taacggtaat gtaattaaca
    22201 catcccacaa ttctacatac catacgtgag aaactcctgc aaatacttca gcagtaagca
    22261 ttgataagca tccaattaac aatatgggga acaaattacc catgatatcc tccattttat
    22321 cttaatttca agactccacc ttctaattca ggtgggatag agcccagtct gagtatacca
    22381 tgttcaacta tggctgaaca agttcactca aataacggaa ggcctggata caatcctaga
    22441 atgatgttaa agctttatat atacggccac ttaaatagaa tcacaacttc aagaaaactt
    22501 gaagctgaaa cacacaggaa tatagaagtt atgtggttac ttagaaaatt aaaacctgat
    22561 gataaatcaa tatccgactt tagaaaggat aacagagaag ctattataaa agtacttagg
    22621 gaatttgttg ccttatgtaa gcaatgggat ttgtttggta tggaaggagt tgctgttgat
    22681 ggctcaaaat tcagggcctc caactcaaaa aaaacaatta taacgataag accttagctc
    22741 gtagaattaa gtatcttaac gaaaagattg ataagcacat ggcagaaatg gatgaaaacg
    22801 acaatggcgt agatgtatgc tataatgtcc aagctgtcgt agacggcaaa cataagctta
    22861 ttgtggattg cgatgtcata aataatcctg cagaccatgg tcagttaagt gaaatgagtt
    22921 tcgtgcgaag aatatttttg aggttgatgg tcttaaagct cttgctgata aagggtacta
    22981 caatgctgat gatcttaaga tatgcgagaa ggaaaaaata acaacatatg ttgcaaggca
    23041 aacattttca aactctacag gagaaaccgg gttttatgca gataaattta aatatgatgc
    23101 tgttcgaata ttcttctacc gtaagggttg gattgccgaa tatgtcgtag tcgtactggt
    23161 tctctatttc tccctcttca catactgtct gcactacgtc tccgtgcccg ttatagagga
    23221 aaaatacacc ttccatataa attctagcag aaggtgtaca tgctgtaaat atttacctca
    23281 cattgttctt ttgttcgatg cctgacacat agcattacca aaagtactaa tttgtgtcaa
    23341 tacctgtcta tctgtcgtgc ctggcacata ttctctagag gtaattttct cttttctgca
    23401 taccattcct tcttttgtcc tacaatccac ataaagcccg agtaccggca acctcagatg
    23461 atagccttcg ttctcagaac gggcaagggc gcgacagcct atctaccgca agactcacag
    23521 tttcaaacag gttattttca ctttggtgtg tgatgttaac tctgtctgaa aactattgca
    23581 agtcatttct acggtaaagt gaaaagcatc aaatgcagaa aaaaaagtgg aggattttcg
    23641 taccccctta tacccccctc tggcgcaaat tttaccacag aagagatttc ctgcaaatcc
    23701 atggttcacc atgaactatg gggtacaagc cggtttatat gttcgttact ggcagtctgc
    23761 aaaaactgtg cgtttcatat atgccttcgt ttccggcaca caaatgttta atgccgtgag
    23821 ccaccgtgtg cccctgtgta gcgttttagt tacttccata aggtgttttg cccctcggca
    23881 aaaatgaaac cggatttggc ggctttctgc gaaagaacct ttcccctcaa aatgcaaccc
    23941 caaaatgtgt ttcgccacct caatttgtgg gtgaaccatg gggtcgaaaa ccgtgcaggt
    24001 taaaggcggg tgcaccttac gatgctcccg ccggttcaca gtggccgaca atgccgacca
    24061 cttaaggatt ttgagtatcc atatccatcc cgtttaggta gtcgaaaatt gaacagggtg
    24121 caccttttag gtggctgtta ttatactatc tccgccaaaa ctcgcatttt ataggatgtc
    24181 tattaaggat accttccact gttaaggaac atattaatgt cattgctaac ctttgtatga
    24241 ttataaagtt ttcctgtcac tctttacatt gttttcctct tgtggttaat cttttagtag
    24301 atcggccacc ttgtgattta catttagcac agagatttta ctatcaatta atgaaccatc
    24361 tgctgtataa atctcaagct catcaacaat tccgcctaat acgtgtagca aaaacattat
    24421 tggatgtctg ccttgttgaa atgcaatcat ttcaacaggc acgcgaactc taaatgggaa
    24481 tttttctttc tcgtcattta cacaaaactt aattgatatg aaaccttcac cgtttattag
    24541 ttcatatttc gagttttcca tttgctttac aagaatatct ctccctttga attcaattgc
    24601 taataattta tttatccata ctgacacatc agcataagaa ggattatcat gctcatgatc
    24661 tttatggcag agggtaatat gttccaacat tagtcacccc ttcactcacg ccataagcgc
    24721 gatactcaat gtttattccg ttgacattaa cggttccagt atacctttct cctacaccta
    24781 attttggtac tttattagca attgtttgtt caactgagga aatatcgact ccagttacat
    24841 gccgtcctcc atgaccaaat gttactgtac catttccagt tttgatactg ttagtaatgc
    24901 cacctccacc attgggagtc tgatatccct tactcccctc gccctgagta gcattatccc
    24961 caaacaactg caggtcaggc ttgaatcctg atgtgctcgg attcaaccct tgcttccaca
    25021 cactaaattt tgccctataa tactctgcta caggagcaat ctctgacagt atatatcgga
    25081 ctgacataat agctgctgcc gattttataa ctgcatccgt catcctaaac agatcaacat
    25141 tagttgttga gtatctattc aaaagcatat ctgcatctga tactatagat cctgacaaga
    25201 actctgtgaa attgttcctg ctgtctggac gatttatatc cgtccattgt cccgcaatgt
    25261 tcttgtggta tcttgattct tttcctgtca gtatctcata aacttcattt ctcaggcttt
    25321 ctctagactc attttgtccg gcacttgctt tctttgatgg tgccagatag tctcttactc
    25381 catgtatgtc ctccaaagct gcaaatgcaa cctgttctga tgtcaagccc aatcctctgt
    25441 tatgttttgc atcggacagt acttcctggt tgtgtatgct gggatgcctt cgattttcta
    25501 tttcagctat tcctacatct ttcccgactc tcttccagag atctctgtta cctgagctat
    25561 taatgaatct gatactgtct tttgagacat ttacaagttc gtctatagat gatactccga
    25621 agtcaacctt taacctattt ctatttacac ctggaacatt gaccccatat gcttctgctg
    25681 ctgtcagttc ttctaatctt ctttctatct tcttgcgctc ttccctgtca accttaccat
    25741 ctttggcaga cctttcaact tcctttttat atttatatcg ttcccaatct cctgcatcat
    25801 caccttttga tttgcctagc ttttcaactt ctttgtttcc cttttccagt ctggcaagct
    25861 catcacgcaa ctcattagca cgtttgttgg catctctctg ggcctgattc caatccttct
    25921 gcccaagtcc gcctcttcct gaatttgctt ctttccacca tttatccttt tcagaatcta
    25981 tttccttgat tatatcatca atcttgctat gccctgtcgg atcaatgaag ttaataggat
    26041 cattctcgca atacgtatac aaattcaagc tcagcgggtt cgtatcctct ccccaatagc
    26101 tgtcttcgct gatgaacctt cctatatatg ggtcatagta tctggccctg agataataga
    26161 gccctgtttc tgcatcatag aattctcccg cataccttat gctgttctgg tagttttcta
    26221 ccgtaagggt tggattgccg aatatgtcgt agtcgtattg gttctctatc tctccgtctt
    26281 cgcttactgt ctgtgctaca tcaccatgtc cgttgtagag gaagtaggac agcttgtctg
    26341 tattatctat ccttgctata tagttgatac cccttacata ccttatttcg tcatttcctc
    26401 tcgcctcaag tataacatgc tgtctgtcat agagatagct tgttgtttct gctgcattgc
    26461 ccttattgga tcttctgact gtcttttccg tcctcagtcc gtccccgtta taggcatatt
    26521 ctactgttgt ctttacccca ccccttatta tctccgtcct taccagtctg ttgaagccgt
    26581 cgtacttgct tacagtctgg tctactactg catggatggg ttctgtggtt tcctcatcgt
    26641 atactgctgc ttcatatacc ttggggttct ccttgttgta gagggatatg tattccacgg
    26701 ttcttctcag ttcattcccg ttatcgtcat acctgaagga ggttatcttc tgcagcagct
    26761 ccatccctcc ggcattcttc agcttctcta caagcttcag cagtctgttg gtattcccgt
    26821 atacatattc gcttctcttt actatatatt ttatttcacc caaacctgtg ccgtcaatag
    26881 caggcaagat ctgttccgat gtataggtct catcctggga tatacggttc ccggctccgt
    26941 cataggtgta taccgttgtc ttgcccgggg cttccacctt taatatcctc ccggccttgt
    27001 cgtaggtata ttctgttgtt ccgtagctgt cggtcctgct tgtctgtctt cctgccttgt
    27061 cataggtata gctgtaagag gatattatac ctcctccggc tttcctgttc accaactcca
    27121 acagcctgtt gttcttatcg taggtatatg cttcttctac ttctccatca tagtcaatgc
    27181 tctttctgtt gccgttttcg tcatattcat attctattgt cctaccctca aagcttacgc
    27241 tttccatcct gctggatctg tcataggtgt attctgttgc gttgcccagc ttgtcagtta
    27301 cacttgctat gtttcctatg tcatcatatg catagcttat ctgtagctct ccgtccttct
    27361 ctatgcttat aagccaattg tttccgttat atgcatagat gcttgtccct gtatcgtctg
    27421 tcatggtctc tctgttgcca agcacatcat aggtgtagga aactgtatct cctgtctccg
    27481 gaactttttt ctccgtcagt agtcctctgt tgtcatagct gtatattgtc tcattgccgt
    27541 ttctgtcttt catggctgca acagccaggg acaggtcata tttataaact gtttcctgtt
    27601 cgtctgcatc gatataggag ataagcaatc cgaagcttcc atagcagtac ttcgttatct
    27661 tgcctcttcc gtctgtcatt gtgagcttgt ttccggcttt gtcccaggtg tacttggttg
    27721 ctataccaag tgcatcagtt acttttacaa gctttcctgc attgtcatat tcatactgtg
    27781 acgtgttccc caatgcatcg gtatgctttg caagctctcc gtactggctg tattcatatt
    27841 ttgtctgccc cccttccgga tcaatagtct ttactatcct gcctgcaaga tcatactcat
    27901 actctatccc atatctttcg acatctgttt ctccggacag atatcccttg gcatctatgt
    27961 cctttattat attgcccttc tcgtcatata ccttcctgtt gattaccttg ccgtaggggt
    28021 caattacttc tattactctg ttgagcttgt catagctgta gcttatggta tccccctttg
    28081 ggtttgtctc gtatgttttg ttccctgccg ggtcataccc gtattcatat gtctttccca
    28141 gagtatctgt catagactct attctgttca tgctgtcata ggtataaacg gtttctaaat
    28201 ccttttcatt tatttctttt accttgtttc ctgcttcgtc gtaaaaatac cgtaccgaca
    28261 cttcacttcc gttatatttc ctgccaatcc tctcaagcct gtttagaata tcatagctat
    28321 atgttactgc atagccagct ccggcttcct catcagcgca gccatatgcc ttggggctta
    28381 tgacctttgt cctgttccct aaaaggtcat actcgtagcc ggtaattata ttaatcttgt
    28441 caggatattc gctgtccctc aattcagcta cgtcatctat tccttccgtc aatatgctgt
    28501 cttcgtctac aagctgtaca gccttcttta ccctgttttc gctgtcatat tcatattttg
    28561 ctatatcata gatgccgttt tccctaaggc tttccgtttt aatcaggtta ccgttattgt
    28621 cataatgata cttgacatta ctgccgctgc tttgaattgt ttcccggagc aatccgttag
    28681 ggtagtatgt ataattggtt atcctctttg acaattgacc atactttgcc gctgtaatcc
    28741 gggtcaagcc tgaaacctat tattcttaca tcaaagggat ttaagttgcc ctaaatccct
    28801 ttgatgtata ttcatttttt gtttgatata tttattcaag agaattagtt tttcagcgct
    28861 tttcctggca cttaactcat gtattattaa tagaaataag ttgtactatt tcatagatat
    28921 ctaaacccat tataatagaa tataaaatta gtgatgtttt actcttagtt ttcagaagat
    28981 atgctatagt agcgcaaaca atactcgtga tagaaaaaat taatacaaca agaactgcat
    29041 ccgaatcaat caagtttgac atatatgtgt cttgcgtcca tttatatcca tgtatcggta
    29101 taaacacatt aatcacccct aaagcataag caacaatgtt tagtaatatg tatactattt
    29161 ttttactcat taagcatatc ccccaacaat gattttcttt ttgttataaa tgttccatag
    29221 ctcatattgt ccctaatttt atctatgctt aggttcggtc ccctattata tcttgttgca
    29281 atcactttca catcatcgat agtgagatgg ctagaaattt tgcctttaaa atctatatct
    29341 cttagttctg ataaatgttt tgcggaaatt agaatgttct gtacaggatc ctttattgat
    29401 ttaattataa tccttctctg actatccgta agattttcta ggttatatcc taaagttctt
    29461 gttgctactt ctacttggat acttacattg ccatatgacg ttaagtcagg attttttcct
    29521 ccaggtattt tgtttctcat accttttgga atgttatccc taacaaaata tgcaacatcg
    29581 tcaatccaaa gcggatcacc acctacctca gaatatacta ctccagcaat taggtattca
    29641 ggtaaatcat acagtttagc agcagcttta ataacttccg catatccttc tacccattgt
    29701 tttttgaaat caaaaaggta tttgtcattt ccttcagttt tactcaggat cttcgatccc
    29761 cacatgccat tgtgccaggc acgtcatata gacagttatc ttccatactt gtctatcagt
    29821 tctttaatcg cacttcccgt tg
//
LOCUS       DAOQUR010000028        29671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1832_length_29671_cov_11.752971, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000028 DAOQUR010000000
VERSION     DAOQUR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 29671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29671
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_29671_cov_11.752971"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..660
                     /locus_tag="PKI50_07380"
     CDS             298..660
                     /locus_tag="PKI50_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNT03558.1"
                     /translation="MKREVMGQCPICSGPVEITEVSCNSCKSTIKGHFKPCKFCSLSQ
                     EHKEFAEIFIKNRGNIKEIERELGISYPTVKGKLDSLISALGYKSQSVDQSYKKEILE
                     RLYRGEISSEEAIRLMSE"
     gene            672..1922
                     /locus_tag="PKI50_07385"
     CDS             672..1922
                     /locus_tag="PKI50_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNT03559.1"
                     /translation="MEEKMLILKMLQEGKITTDEAVKLLEALEKGGSGTPSSGSKINE
                     IKDELAAKLNEIKIDEKLNKFGEKASKLASTFGEKAGKLVEQLGENINTDKTGNNAEK
                     FAEEFTKRMEILGQDIAESAAKFADVFTSQIDNLFDTVYEKHKYNSSYTYPVEDTLSL
                     YLETGNFSVKACPVDTKEIKINLYVNSNVPQLAIDEYFKVIKDTEGYRLSTEFPGRTW
                     GRIEILLPKGMDVISFSTNNAKCEISDIDANHISCATANGRVILTRCSADEIEIFTDN
                     ERIIFNEVSARTANIRTSNSKIAVEDSRLDNIDAKTSNAAIAVSTSRKGDSLNPCYTL
                     STSNGKIDFSLAGEEGFEYMVDAHTAMSSIDVKLANLTYETEKKNIGMQGSVQLKSDN
                     FDTASNKVSITANTTNAPISIRNI"
     gene            complement(2041..2673)
                     /locus_tag="PKI50_07390"
     CDS             complement(2041..2673)
                     /locus_tag="PKI50_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03560.1"
                     /translation="MNKKNALIGIALIFLGISMYLKNYNIGTGSLLTLFLGLGLLYAY
                     HIKREQPFIIFGGIFAAIGLAAVLRDIRLFRIDMVFEAMLIALGIIFIFLYYSKHIEG
                     FIFPGVILPAAGVYLLLLRSFNDRYVSPSIFLLLGFAFYVIYLIAYMGKSNWPLIPAN
                     ILLMAGIFAYAVSFEIITWHMVYLKRDYIWPLLMIGAGVLILISRFRRRA"
     gene            complement(2700..3329)
                     /locus_tag="PKI50_07395"
     CDS             complement(2700..3329)
                     /locus_tag="PKI50_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNT03561.1"
                     /translation="MNWIDYLIIAILAVGVIIGFRKGFLKSVFSIICLLVSIMVAKTY
                     YKALTMFLVENTALEEKITGFLSEKAFVKNILMSPSGESAVFSISNSFTSDLNAFVTV
                     LIINAISVLIIFIAVRFILGIAESILLDAVELPGLREVNSIGGAIVGLAKNIIIIMLI
                     FTIITPISAIKALSGISDGIEASALAEYFYAYNFILGWIWTAAVDLLNK"
     gene            complement(3351..4448)
                     /locus_tag="PKI50_07400"
     CDS             complement(3351..4448)
                     /locus_tag="PKI50_07400"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HNT03562.1"
                     /translation="MQKVRSKKTTALLLLLIIVSVLAILLYINNKTTRKSSFIAGGKS
                     KLAVTGEVTAVSQSSSAIYYVKDLLIEAQAENIRAKNINGDIAWSCKLPGKAVKMVDA
                     GQNIIIIDSANNIHYYTLQGKLLWTYKSDYEIVDIFTEDNGSFLAEYKGMTGSHAEAF
                     KQDGSKIGSISVDNAHILSFAGGSGVFSISIVDTSSEMIKTKIITYSFKGDILWAQNF
                     DNVIISKLSFSKDNRLLALGENTVYIYKNDGSLHQEVKIEGEISRAAMNGHIVALALE
                     IEDKQYLVCYDANMREQSRTEIKPEPLGIFPLKSNLIVYYSDELMILTLKGELNARYE
                     PNVDISAAYMTSDNKVYIASNRRLLQLEYIK"
     gene            4709..5356
                     /gene="yyaC"
                     /locus_tag="PKI50_07405"
     CDS             4709..5356
                     /gene="yyaC"
                     /locus_tag="PKI50_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HNT03563.1"
                     /translation="MLKIKYERTVDINKPYSFSYFCNYLEECMSIFYDKNHTDIVIVC
                     IGTDRATGDCLGPLIGYKVKDMRYKNVHVYGTLDEPVHAKNLCEYMELFRKFDNPFII
                     AIDACLGRYERIGFINIKEGPLSPGSGVNKNLPAIGHINITGIVNVGGFMEIMILQNT
                     RLSVVMNMASLIANGLKYNMWKMNRRALSDNSLINRQIASTASEDKFFDYMENKN"
     gene            complement(5361..6530)
                     /locus_tag="PKI50_07410"
     CDS             complement(5361..6530)
                     /locus_tag="PKI50_07410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT03564.1"
                     /translation="MYQGVYDRILLIAMTCFSFFIALYTISMNKTVSLFKLLGWVFFI
                     PFGLCCSIFVFSPDTPINLILSFSEFCLAAGILLLILYPYNTKNQKLMIVVIAALTLI
                     LSVYAIKTEHFIYYTFSAALRNILTAACIIAAIVFNIKKNADRKLAKPLAIWLTGMLL
                     GFVENKAYINEAVFAVKLSAYACFLFYFFRTTHDLLMKKVNESEELIEDMKKSLNKEV
                     KKRVFEIERTNEMLLEISKTDMLTKTFNKITILNIIEKLITSKKMEAFSILMFDIDNF
                     KTINDSLGHVTGDMCLKTLSAIASGNIREVDYIGRYGGDEFIIVLPTLSVNEARFVAE
                     RFRKKVSETSNPRFTVSIGIATYPHDGQTVHELIQAADRGLYKAKSMGRNTVCRA"
     gene            complement(6639..6917)
                     /locus_tag="PKI50_07415"
     CDS             complement(6639..6917)
                     /locus_tag="PKI50_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HNT03565.1"
                     /translation="MIIAIQHGLEDIKKELESRGNEVFYIGENRMADAVLYKEADTRP
                     YYAVNNVPSTAALSNENISYGALLVNVTNKSMDDIIRILEKRIYSPLL"
     gene            complement(6984..7490)
                     /locus_tag="PKI50_07420"
     CDS             complement(6984..7490)
                     /locus_tag="PKI50_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HNT03566.1"
                     /translation="MNIMDYFIKVYNAEILFSITILLFIMIVWNLILSVRLAGMSEKY
                     KKFMRGSSGKNFEGMLLGHIENMEASIERVNIISEDLIHVKNQMDKCIQKCNIIRYNA
                     FSDTGSDQSFSIAMLDNFNDGIIITGIYGRNEFVGYAKPVSKGTSRYPLSVEEELVLN
                     RSLKGTQI"
     gene            complement(7502..8644)
                     /locus_tag="PKI50_07425"
     CDS             complement(7502..8644)
                     /locus_tag="PKI50_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT03567.1"
                     /translation="MIYLDNAATTFPKPESVYRAVDDCLRNYCANPGRGGHKLSLQSG
                     RVLLEARELLAELFNADSPDNVILTHNATEALNLALKGFLKKGSHVITTSMEHNSVMR
                     PLKVLEKLGIETTIAQCNEYGEIDIGSIEKEIKKETALIAAAHASNVVGTIIPIEDIG
                     DLAKRYGLAFLVDASQTAGVYEIDMQKMNISMLAFAGHKSLMGPQGTGGLCIKRGLEL
                     TPLQEGGTGSKSESLYQPDIMPDRFESGTHNTPGIAGLCAGLSFIKSKGIDKIRAHEQ
                     ELVKYFLDGLAEIKNVKVYGVKEASKQAPVVSINIGDTGSSEISYILDRTFDIATRSG
                     LHCAPMAHKTLGTLEQGTVRFSFGFFNTKEQVGAALNAVENIGTLL"
     gene            complement(9314..10177)
                     /locus_tag="PKI50_07430"
     CDS             complement(9314..10177)
                     /locus_tag="PKI50_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNT03568.1"
                     /translation="MQQYILSSFVSNRGGGNKAAVVFDDFGMSYWQMLELAKKNNFSE
                     TAFIDFNKSDGNRFSIRYFTPAEEVDICGHAALASFQVLKQLGYLRDNYAYHRTKAGE
                     LKVLIEGDSILIQMNKPELIMQLSSNIIMDIIDAEKDEITTNRNGMIDVISTGLKDII
                     LEVNGIEALKKLRIKKDKMIEICREYDIIGMHVVSRETIEEDSDFCCRNFAPAVGIDE
                     ESATGTSNASLLYYIKRNDKQFDCGKTYKVEQGYFMNSPSNIFVKYDQGNESIFVGGI
                     ARIDSMESVVI"
     gene            complement(10292..11134)
                     /locus_tag="PKI50_07435"
     CDS             complement(10292..11134)
                     /locus_tag="PKI50_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNT03569.1"
                     /translation="MISRRGLGKGLGALIPEGEESDRNSIVEIKITDIEANDKQPRKA
                     FNDATLADLSESIKEHGVVQPIIVRKQGNGYQIVAGERRWRAARLAGKKTIPAIIKEC
                     SNLEVMELALIENLQREDLNSIEEAMAYKSLIEEYNMTQEEISKKIGKSRPAIGNSLR
                     LLQLPQEIKNMIAEGKISQGHARALLSISGEKKQLDMAEKIIAQQLNVRQIEKLAKDT
                     KQKKKKEVLPDAYRIEINQLEERLRAVLGTKVTIHHRKNRGSIEIEYYSDEEFDRIFE
                     LLEK"
     gene            complement(11135..11908)
                     /locus_tag="PKI50_07440"
     CDS             complement(11135..11908)
                     /locus_tag="PKI50_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT03570.1"
                     /translation="MSKSIAVFNQKGGVGKTTTVINLCACLANRGKKVLMIDVDPQGN
                     TTSGVGIDKTTLEKTIYDVLINGSDPRECIIDTNRENLFIIPSSVQLAGAEIELTEMN
                     KRELKLRECIESIKDEYDYVFIDCPPSLGLLSINALSAVDSVLIPIQCEYYALEGVSQ
                     LMNTIKLVKRSLNPDLEIEGVVLSMFDGRTNLSIQVVDEVKKYFRGKVYTTMIPRNVR
                     LAEAPSYGMSIIEYDMKSKGAESYMDLADEFIELSEDVI"
     gene            12180..12449
                     /locus_tag="PKI50_07445"
     CDS             12180..12449
                     /locus_tag="PKI50_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNT03571.1"
                     /translation="MLKSVLYTFFLVFIAELGDKTQLATMLLSAKSNSIIAVFIGASF
                     ALICSSFIGVFAGAYITKHVPPQYIQNAAGVLFIAMGLLILKGKI"
     gene            complement(12490..13284)
                     /gene="noc"
                     /locus_tag="PKI50_07450"
     CDS             complement(12490..13284)
                     /gene="noc"
                     /locus_tag="PKI50_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HNT03572.1"
                     /translation="MNDRKEIINIPIDEIAPNPYQPRKEFNGSSLEDLASSIKEYGVL
                     QPINVRKIGEKGYELISGERRLRSSKLAGKSHIPAVIIEVVEQDSAVIALIENLQRED
                     LNFIEEAEGYHNLINDHGMTQEELARKVGKKQSTIANKLRLLKLKDSIKKTILENELT
                     ERHARALLKLPDDTLQEKALKSIIKKSLNVKKAEELVDKMLDEAAAAAEEPKKKRIKG
                     KMNYNIYVNTLKNACKEFLTAGCKVEYGQVDKGEFIEITVKIPKNV"
     gene            complement(13417..14136)
                     /gene="rsmG"
                     /locus_tag="PKI50_07455"
     CDS             complement(13417..14136)
                     /gene="rsmG"
                     /locus_tag="PKI50_07455"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNT03573.1"
                     /translation="MENRKILIDGLTELGIEPANKKVDKLVAFKEIMLEWNEKVNLTA
                     ITEEKEIFIKHFLDSATCLSAGYIKEGMSIIDVGTGAGFPGIPVMILMDGLKMTLLDS
                     LNKRVIFLSEAIKKLDLRDITAVHGRAEEAGGNKAYRECYDIVLSRAVASMNVLCEYC
                     IPFAKIGGYFLCQKGPEIEEEIGNAASAVKILGGTIKEVKEYKLPFSDIRHNIIVIEK
                     VSATPTKYPRKPGKPASNPIT"
     gene            complement(14111..16024)
                     /gene="mnmG"
                     /locus_tag="PKI50_07460"
     CDS             complement(14111..16024)
                     /gene="mnmG"
                     /locus_tag="PKI50_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNT03574.1"
                     /translation="MEYTAGKYDVVVIGAGHAGSEAALACARLGLKTLVTAINLDSIA
                     MLACNPSIGGPAKGNLVREIDALGGEMGINVDRTMLQIRMLNTRKGPAVRALRAQIDK
                     KLYQDRMKRVMENQENLQIKQAEITRIIVENDRVKGVVTHLGAIFECRSVILATGVYL
                     KGKVVIGEISYDGGPNGLFPAAKLSDSLEDLGIKLLRFKTGTPARIDGNTVDFERMTE
                     QPGDRDINTFSFMTGDIDIDQLSCYLTYTNKGTHDIIRKNLHRSPLYTGDIVGVGPRY
                     CPSIETKIVSFPDREEHQIFIEPEGLNTREMYVQGMSSSLPEDVQLDILHSVKGLENC
                     HMMRTAYAIEYDCIDPTQLQLSLEHKKISGLFFAGQINGTSGYEEAAAQGLIAGINAA
                     LSLRGKEPFILDRSEAYIGVMIDDLVTKGTNEPYRLMTSRAEYRLILRQDNADLRLTQ
                     KGYDIGLVSEERYKRYLEKKNNIEKETDRLKKKSISMTEDIREFLMSRESAEIKGGIS
                     LYELLKRPEIEYCSLKEIDEEMPELDRETAEEIEIMIKYEGYIKKQLQQAEQFKKLES
                     RKIPENINFADIKGLSLEARQKLDSINPANLGQASRISGVSPADVSVLMVYLEQIRRG
                     RGGEQAGKQENTD"
     gene            complement(16035..17414)
                     /gene="mnmE"
                     /locus_tag="PKI50_07465"
     CDS             complement(16035..17414)
                     /gene="mnmE"
                     /locus_tag="PKI50_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNT03575.1"
                     /translation="MFERTIAAIATAPGEAGIGIVRISGEKSTEILKKVFKPKRVMDI
                     DDIPSRTVVYGHAVDSKGAIIDEVLVIVMRKPYSYTMEDVVELHCHGGIVPIRRIMEE
                     VLRNGADIAEPGEFTKRAFLNGRIDLAQAEAVIDVITAKTETGLNAALNQLEGELSKE
                     IKKIMEKLLSMLAHIEASIDFPEHDVEEITTANIKNMLLEAKASVMELAESFDEGKIV
                     RDGLSTAIIGRPNVGKSSLLNVLLKENRAIVTEIPGTTRDIIEEYLNMGGVLVRLIDT
                     AGIRDSEDIVERIGVERTKGAISNSDLIILVLDASDELRQEDNEIIDLIKNKKVIAVL
                     NKTDLGLVIDEAYLRGVFGEDNIIRMSVKNRIGIDKLEERIKGLVYHGKLSISKNSMV
                     TNIRHKNLLDRALENIERAILSIEDNIPVDLISVDIREAWNSLGAITGDTVEEDIIKE
                     IFSKFCIGK"
     gene            complement(17700..18392)
                     /gene="jag"
                     /locus_tag="PKI50_07470"
     CDS             complement(17700..18392)
                     /gene="jag"
                     /locus_tag="PKI50_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNT03576.1"
                     /translation="MMMVEKTARTVEEAVQSALEELETEKDNVDIEILEQPTKGIFGI
                     LGSKNAKVRVTLKEKKTTEKSVKKEIENKEIRITEYTLEKERARKFLREVLEAMDIKA
                     EIRVKNINESLYINLSGPKMGMIIGRRGQTLDSLQYLVSLVVNKDKERDSFVKVILDT
                     EDYRRKREETLQRLAKRLADRARKTGKNIELEPMNPYERRIIHSTLQEVEGITTFSEG
                     EEPYRKVIISNK"
     gene            complement(18398..19126)
                     /locus_tag="PKI50_07475"
     CDS             complement(18398..19126)
                     /locus_tag="PKI50_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNT03577.1"
                     /translation="MLDFIAEPLGALLKFIFDFVGDYGYSIIIFTILTKVLLLPINIK
                     QTESTKKMNELNPKMKEIQEKYKNDKEKMNQKLLQLYKEQNYNPASGCLPALIQMPIL
                     FALFYVIQNPVKYVFKDANIYAGIAKNFWWLLDLGKPEMQATMIKVAGFGVPILAVLS
                     AATTYYQMKMISAPKANKKKGKEDTAAQTQSMMTNIMPIMFGWITLNVPSGLALYWVA
                     GNIFTIIQQYFMMKPKPQAAKKEE"
     gene            complement(19224..19448)
                     /gene="yidD"
                     /locus_tag="PKI50_07480"
     CDS             complement(19224..19448)
                     /gene="yidD"
                     /locus_tag="PKI50_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNT03578.1"
                     /translation="MRKILIVIIKTYQLLISPLSRGKCRFIPTCSSYAIEAIDRHGVI
                     RGLYLSAGRILRCNPFNPGGYDPVPEKKNR"
     gene            complement(19449..19793)
                     /gene="rnpA"
                     /locus_tag="PKI50_07485"
     CDS             complement(19449..19793)
                     /gene="rnpA"
                     /locus_tag="PKI50_07485"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNT03579.1"
                     /translation="MTTLHKLKKNYEFKKVYNEGKYYVEKFVVMYIIPNDSNFNRVGF
                     SVSKKVGKSVIRNRIKRLMKEAYRKFADTVKLGYDIVFTARAGSGSADYSMIENNIIS
                     ILKRAKLKKSEE"
     gene            complement(19841..19975)
                     /gene="rpmH"
                     /locus_tag="PKI50_07490"
     CDS             complement(19841..19975)
                     /gene="rpmH"
                     /locus_tag="PKI50_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNT03580.1"
                     /translation="MLMTYQPKKRQRKKVHGFRERMSTATGREVLRRRRRKGRKRLTV
                     "
     gene            20500..21831
                     /gene="dnaA"
                     /locus_tag="PKI50_07495"
     CDS             20500..21831
                     /gene="dnaA"
                     /locus_tag="PKI50_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNT03581.1"
                     /translation="MYSQLVEIWEKTLSVIKVELTEVSFNTWLKAIQPVSIKENLITF
                     SVPDDFTKGILEARYSKLIKNALKQVTSEYYDIRLIIPTEENFTSIMTERSEINQSEE
                     SVNSSLNPKYTFETFVIGNSNRLAHAASVAVAESPAKAYNPLFIYGGVGLGKTHLMHA
                     IGHYILKQNMRSKVVYISSETFTNELINSIRDDRNNEFRSKYRNVDVLLIDDIQFIAG
                     KESTQEEFFHTFNSLYEANKQIIISSDRPPKEIRTLEERLRSRFEQGLIADIQPPDLE
                     TRIAILRKKAKSENLSISDDVMLLIAKKIHSNIRELEGALIRVVAYSSLTNGEINEEL
                     ANEALKDILSNSKPKKVTPSAIKDVVGNYYNIKIEDFNAKKRNKSVVLPRQIAMFLCR
                     ELTDLSLPRIGEEFGRDHTTIMHAYEKISSDIKIDTQLKSVLEELKSKIEG"
     gene            22060..23166
                     /gene="dnaN"
                     /locus_tag="PKI50_07500"
     CDS             22060..23166
                     /gene="dnaN"
                     /locus_tag="PKI50_07500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNT03582.1"
                     /translation="MKIITTKSTLLESINVVQKAVPSKTTLPILEGILFDAKSGKLKL
                     TATDLEMGIETTSNVDVIEPGKIVLSSRMVGEIVRKLPDSDVEIETAEGNLVSIKSEG
                     SKFKIVTLPYDEYPELPVVKKENGITIKQNILKDMIRKTIFAVSTDEIRPILTGVLFD
                     VSGEELTMVALDGFRMAVKKNVIMNDNNFKAVIPGKTLSEIGKIINDNEENINIYFSK
                     NHIQVQIEDTIIISRLLEGEFINYKQIIPAEYKIKTKVSTNLLMEACDRAALFARDSS
                     NNMIKFEINDDMMVIMSNSQNGDVHEELKIQKEGSDIEIAFNAKYFIDVLKVIESEEI
                     TIEFTTNVSPSIIRPVKDEEYLYLVLPARFKKNS"
     gene            23235..23441
                     /locus_tag="PKI50_07505"
     CDS             23235..23441
                     /locus_tag="PKI50_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNT03583.1"
                     /translation="MREAKIETDFIKLDQFLKFENIAGTGGEAKNMIMDGLVKVNGNI
                     EKARGKKLRRGDVIEIFGEEIKLI"
     gene            23463..24569
                     /gene="recF"
                     /locus_tag="PKI50_07510"
     CDS             23463..24569
                     /gene="recF"
                     /locus_tag="PKI50_07510"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNT03584.1"
                     /translation="MYIKRIDLKNFRNYKEFDIELGKGVNIFYGQNAQGKTNIIESIY
                     MASTGKSHRTQKDMELIRWNSEDAKIRIDYQKKEEEKIEIYLNKSLKKQIKKNGVRLF
                     KIGELIGNLNTVIFSPDHMKIIKEGPAERRRFIDIILSQVKPGYYYNLVQYLRTLEQR
                     NNLLEEARTNSGILKTIDIWNEQLADFGARLIKARQDFICDICCMAADIHKKISNGKE
                     TLVIKYNSSIKYHSNSEKEIKENFIIALENHINADMGRSATHKGPHRDDILFFINGTE
                     VKTYSSQGQQRTTLLSLKIAELKYMKEETGELPVLLLDDVFSELDDERQKYLIEFIKD
                     MQAIITCTNVEHLEKLMLEGSRTYKVESGMIVKD"
     gene            24651..24920
                     /locus_tag="PKI50_07515"
     CDS             24651..24920
                     /locus_tag="PKI50_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNT03585.1"
                     /translation="MFMHLGGDVVISLKDIISIMDIESSSTSSITKEFLKTAEDEGFI
                     RRISEDEEKSFILTEKNKKTIIYLSPISSVTLFKRAGFIKDISLK"
     gene            24934..26844
                     /gene="gyrB"
                     /locus_tag="PKI50_07520"
     CDS             24934..26844
                     /gene="gyrB"
                     /locus_tag="PKI50_07520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNT03586.1"
                     /translation="MEENKFEGTYEAEQIQVLEGLEAVKKRPGMYIGSTGSRGLHHLV
                     WEVVDNSIDEALVGACKNIKVTIHPDNSISNEDDGRGFPVGMHPKLNKPAVEVALTVL
                     HAGSKFGGGGYKVSGGLHGVGVSVVNALSEWLEVEVKRDGKIYHQRYERGFPVTELKV
                     IGTTNRTGTRVSFKPDSDVFDELEYSFDILEHRLRELAFLNKGIKISLKDERDGTEKT
                     FQYEGGIISFVKHLNKNREVLHKEPIYFEAKKDNVDVEIALQYNDGYAESIFSFANNI
                     NTLEGGSHMSGFKNAVTKVINDYARKYKFLKENDANLIGEDVREGMSCVISIRLTDPQ
                     FEGQTKTKLGNPEVRGIVDSIVTENFEAFLEETPSNAKIIVEKALTAARARDAAKKAR
                     ELTRRKNVLESTTLPGKLADCSERDPSLCEIYIVEGDSAGGSAKQGRDRRYQAILPLR
                     GKILNVEKARLDRILNSEMIRAMITAFGAGISDDFNLEKMRYGKIIMMTDADVDGAHI
                     RTLLLTFFYRYMSPLIEAGYVYIAQPPLYKVRKKNKDYYAYNDKELEKVFKEIGREGS
                     SIQRYKGLGEMNPEQLWDTTMNPETRIILQVTMEDAVAADEIFTVLMGDKVEPRREFI
                     EHNAKYVRNLDI"
     gene            26868..29309
                     /gene="gyrA"
                     /locus_tag="PKI50_07525"
     CDS             26868..29309
                     /gene="gyrA"
                     /locus_tag="PKI50_07525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNT03587.1"
                     /translation="MDYENQKILPINIEEEMKKSFIDYAMSVIVQRALPDVRDGLKPV
                     HRRILYAMNDLGITPDKPHRKCAMTVGEVLGKYHPHGDSSVYDAMVRMAQDFSQRYML
                     IDGHGNFGSVDGDGAAAMRYTEARMSKIAMELLKEIDKETVDFTPNYDESLKEPTVLP
                     ARFPNLLVNGSNGIAVGMATSIPPHNLGEVIDCAVSTIENPDIELEEMLKYIKGPDFP
                     TGAIIMGKEGIKKAYSTGRGKVIVRAKAEIEEMSGNRQRIIVSEIPYQVNKAIMIEKI
                     ADLVKEKKIDGISDLRDESDRNGMRVVIELKRDANANIILNQLYKHTQMQETFSIIMI
                     ALVNGQPKVLNLKSVITHYIDHQKDVVTRRTQYDLKKAEARAHILEGLRIALDNIDEI
                     VSILRNSKNVAEAKERMMSRFGLSEIQAEAIAEMKLKSLTGLERGKIEEEYLSIIKNI
                     EYFRSLLANEQKLLMVIKDELLEIKKKFNDKRRTKIIPTSLEEFDEEDFIEEHEVAVT
                     ITHFGYIKRLPADTYKSQRRGGRGITGLQTREDDFVENLFITSTHNYILFFTNKGKMY
                     KLKTYEIPEAGRQAKGTALVNLLQLDAEEKVNAVIHIKDFNDGKYLIFMTKHGTIKKT
                     ELKEYDTSRNTGITAINLIDNNELIGVKLTRGTTELIAATKKGLAIRFPESDVRPMGR
                     SARGVRGINLNRDDVVIGMDIVEEGADLLVISEKGYGKRTPIDEYRVQTRGGKGIITS
                     RITPKTGHVVGMKIVKEQDEIMLINLSGIIIRMNVNEISKMGRSTQGVKLMRVPENES
                     VVSIAKISSNEEEEK"
BASE COUNT         9100 a         6053 c         5402 g         9116 t
ORIGIN      
        1 ctcgggtagg tttttgcaag tgtgcaaaaa ccctcattaa aatcttgtta taatatttga
       61 attatatcat taattattaa tttttaaaac taaagcagca tgttttgcgt atatataaat
      121 aagcagagat tctatacagg ctttccatat ttattaataa tatttatatg cgtttactat
      181 tttttaatat ttccaataaa ttaaattaat aatgttaatt tatatattga cattattaat
      241 tattgggcta taatgggata taacggcaat acattattaa tagttaggag tgaagtaatg
      301 aaacgtgaag tcatggggca gtgtcctata tgcagcggac cggtggagat aacggaagtg
      361 tcctgcaatt catgcaaaag cacgataaaa gggcacttca agccgtgtaa attctgttcc
      421 ttatcacagg agcacaagga atttgcggaa atattcatta aaaatagagg aaatataaag
      481 gaaatcgagc gggagctggg aatatcatac ccgacagtga agggaaagct ggattctctc
      541 atttcggcat tgggttataa gagtcagagt gtggatcaga gctataagaa agaaattctt
      601 gaaaggcttt ataggggtga aatatcctcg gaagaagcaa tcagattaat gagtgaatag
      661 ggggaaatat tgtggaagaa aaaatgttaa tacttaaaat gctccaggag gggaaaataa
      721 ccaccgatga agctgtaaag cttcttgaag ctttggagaa gggcggctcc ggcacaccgt
      781 cctccggttc aaaaataaac gaaataaaag atgaacttgc tgccaagctt aacgaaatca
      841 aaatagatga gaaactgaac aaatttggtg aaaaagcctc aaagcttgct tccaccttcg
      901 gcgaaaaggc aggaaagctg gtagagcagc tgggagagaa tattaacacc gataagacag
      961 gaaataatgc tgaaaaattt gccgaggaat tcacaaaaag gatggaaata ctgggccagg
     1021 atattgctga gtctgcagca aaatttgcag atgtctttac aagccagata gacaatctct
     1081 ttgacacagt gtacgagaag cataaatata acagcagcta tacctacccg gtggaagaca
     1141 ctctcagctt ataccttgaa acaggtaatt tctccgtaaa agcctgtccc gttgatacaa
     1201 aggaaataaa gataaacctg tatgttaatt caaatgtgcc tcagctggcc attgatgaat
     1261 acttcaaggt cattaaagac accgaaggtt acaggctttc caccgagttt cccggcagaa
     1321 cctgggggag gattgaaata ctgcttccca aaggtatgga tgtaattagc ttttctacaa
     1381 acaatgcaaa atgtgaaatc agtgatatag atgctaatca tataagctgt gccacggcaa
     1441 acggaagggt aattctgaca agatgcagcg cagatgaaat tgaaatattt acagacaatg
     1501 aaaggataat ctttaacgaa gtatccgcaa gaactgcaaa catcagaacc tcaaattcca
     1561 aaatagctgt agaggacagc cgcctggaca atatagatgc aaaaaccagc aacgctgcaa
     1621 ttgcggtaag cacttcccgc aaaggtgatt ctctgaaccc ttgctatact ctcagcacct
     1681 ctaatggaaa aattgatttc agccttgccg gagaagaagg ctttgagtat atggttgatg
     1741 cacatactgc aatgagcagt attgatgtaa agcttgcaaa cctcacttac gaaacggaga
     1801 agaaaaacat aggcatgcag ggctctgtac agctcaaaag cgataacttt gataccgcct
     1861 ccaacaaggt atcaattacg gcaaatacca caaatgcacc gataagtatt aggaatatct
     1921 aattagaaca aaaggacacg gggacgtttc gaggacatgg ggacgtttct cctgtcccgc
     1981 tttcagcggg acaggagaaa cgtccccatg tcctcgaaac gtccccgtgt cctctagatg
     2041 ctaagccctt cttctgaacc tgcttatcag tatcaatacc ccagctccaa tcatcagcag
     2101 aggccagatg taatccctct ttaaatagac catatgccat gttattatct caaagcttac
     2161 ggcatacgca aatatcccgg ccatcagcag tatgtttgcc ggtatcaacg gccagttgga
     2221 cttccccata taggctataa ggtatatcac ataaaaagcg aagccaagaa gcaagaatat
     2281 gcttggtgat acatatctgt cgttgaatga tctcagtaat aaaaggtata ctcctgcagc
     2341 gggaagtatc acccccggaa aaataaagcc ttcaatatgt tttgagtaat ataagaatat
     2401 aaatattatt cccagcgcta taagcattgc ttcaaaaacc atatcaatac ggaacaatct
     2461 gatatccctt aataccgccg ccaagcctat agctgcaaag attcctccga atataataaa
     2521 gggctgttct ctcttaatat gatatgcata aagcagtccc agcccaagga aaagagtcag
     2581 caagctgcct gttccgatat tatagttttt caggtacatg gatatgccca ggaatatcag
     2641 ggctatccct atcagtgcat ttttcttgtt catgaattca acaactcctt aagacttgac
     2701 tatttattca aaaggtcaac ggctgcagtc caaatccatc caagtatgaa gttataagca
     2761 taaaagtact ccgcaagggc agatgcctct attccgtctg agatccccga aagagccttt
     2821 atagcagaaa taggagttat tattgtaaat atcagcatta ttattatgat attttttgca
     2881 aggccgacta ttgcccctcc tatactgtta acttctctca gtccgggtaa ttcgactgca
     2941 tctaaaagaa tgctttcggc aattccaaga ataaatctca cggcaataaa tataatcagc
     3001 acggatattg catttattat aagtactgtt acaaatgcat tcaaatcgct ggtaaagctg
     3061 ttactgatgg aaaaaaccgc actttccccc gaaggagaca ttaatatgtt ttttacaaaa
     3121 gctttttctg aaagaaaacc tgttatcttc tcttccaatg cagtattctc caccaaaaac
     3181 atagttaagg ctttatagta agtttttgca accataattg ataccaacag gcatattatg
     3241 ctgaataccg acttcaaaaa ccctttcctg aagccgataa tgacaccaac ggcaagaatt
     3301 gcaattatca agtagtcaat ccaattcata ggtttttggc ctcctttgtg ctattttata
     3361 tactccaact gaagaagcct tctgtttgaa gctatgtaca ccttattatc cgaagtcata
     3421 tatgcggcgc ttatatccac attgggctca taccttgcat taagctctcc tttaagggtg
     3481 agtatcatca attcatcaga atagtatact ataagattgc ttttcagcgg aaatattccc
     3541 aatggctcag gctttatctc cgtacggctt tgctcccgca tattggcatc atagcatacc
     3601 aggtattgct tatcttcaat ttccaatgcc aatgcgacaa tatgaccgtt cattgccgca
     3661 cggctgattt caccttcaat tttgacttcc tgatgaagac tgccgtcatt tttataaata
     3721 taaaccgtat tttctcccag ggcaagcagc ctgttatcct tactgaagct gagctttgag
     3781 ataatgacat tgtcaaaatt ttgagcccac agtatatccc ctttaaagct gtatgttatt
     3841 atttttgtct taatcatttc tgatgaggtg tctacaattg aaatcgaaaa taccccgctg
     3901 cctcctgcaa atgataatat atgagcattg tccacagata tactcccaat tttacttccg
     3961 tcctgcttga atgcttcagc atggcttccc gtcattccct tgtattctgc caggaagctt
     4021 ccgttatcct ccgtaaaaat atctacaatc tcataatccg acttgtatgt ccaaaggagt
     4081 ttcccctgaa gtgtataata atggatgtta ttggctgaat caataattat gatattttgc
     4141 cctgcatcaa ccatcttgac ggccttcccg ggcagtttgc agctccatgc aatatctcca
     4201 tttatattct tcgctctgat attttctgcc tgtgcttcaa taagaagatc cttcacatag
     4261 tatatggcgc tgctgctttg cgatacagcc gtcacttctc cggtaacagc aagtttgctt
     4321 tttccgcctg caataaaaga agacttccgg gttgttttgt tgtttatgta aagtagaatg
     4381 gcaagcaccg atactatgat cagcagaaga agcaatgcgg ttgttttttt cgatcttact
     4441 ttttgcaaat caagcacctc tcataatata tacgaataat ttccaaaaaa gtattcataa
     4501 aaactcatga tttatttgat tgatcatatg ggcacaaagg ccttggatgc agttgtttga
     4561 aaacatgtgg ttattgtatt tgatattgaa taatataatt atacaataaa acactttgcg
     4621 ccacaccctg ttaattttaa aacctttgaa tttactcaat gtgttttggc aatactctat
     4681 gtagatattt cagataaagg agttcggaat gttgaagata aaatatgaga gaacagttga
     4741 tattaataaa ccttattcct tttcttattt ctgcaattac ttagaggaat gcatgagtat
     4801 tttctatgat aaaaaccata ccgacatcgt aattgtgtgc ataggaacag acagagctac
     4861 cggcgactgc ctgggcccgt tgatagggta taaagtgaag gatatgaggt ataaaaatgt
     4921 acatgtttat ggtactctgg atgagcctgt ccatgcaaag aacctttgtg agtatatgga
     4981 gcttttcagg aaatttgaca atccctttat cattgccatt gatgcatgtc tcggcaggta
     5041 tgaaaggata ggcttcataa atataaagga aggccccttg tctccgggct cgggtgtcaa
     5101 taagaacctt ccggctatcg gtcatataaa tatcacaggg atagtaaatg taggcggttt
     5161 tatggagata atgattcttc aaaatacaag gctcagtgta gttatgaaca tggcaagcct
     5221 tatagcaaac ggccttaaat acaatatgtg gaaaatgaac agaagggcgt tatccgataa
     5281 ctcgctgata aaccggcaaa ttgcttccac agcaagtgaa gataagtttt ttgattatat
     5341 ggaaaataag aattaataaa ctatgcgcgg caaacagtgt ttcttcccat gcttttagct
     5401 ttgtataatc ctctgtctgc agcctgtatc agttcatgta cggtctgtcc gtcatgaggg
     5461 tatgtcgcaa tgcctattga tacggtgaat ctgggattgc ttgtttcgct taccttcttc
     5521 ctgaagcgct ctgccacaaa tctcgcttca ttcaccgaca aggttggaag tacaataata
     5581 aactcatccc ctccatatct tcctatgtag tctacttccc tgatattccc tgaagcgata
     5641 gcagacagag tcttcaggca catgtctccg gtaacgtgtc ccaaggaatc atttattgtt
     5701 ttgaagttat ctatatcaaa cattaatatt gaaaaggctt ccattttctt gcttgttata
     5761 agcttttcaa tgatattaag tattgtaatc ttattaaacg tctttgtgag catatctgtc
     5821 ttggatattt ccaagagcat ttcattggtt ctttcaattt caaatactct tttctttacc
     5881 tctttattaa gggacttttt catgtcttct atcaattctt cagattcatt gacctttttc
     5941 ataagcaaat catgggttgt cctaaaaaaa tagaatagaa aacaggcata tgcacttaac
     6001 ttgactgcaa atactgcttc atttatataa gctttgtttt caacaaatcc cagcagcata
     6061 cccgtaagcc aaatagccaa tggcttagca agtttcctat ctgcattctt ttttatattg
     6121 aacacaatag cagcaataat gcatgctgcc gtgagaatat ttcggagagc agctgagaat
     6181 gtatagtata tgaaatgctc cgtttttatt gcatatacgg acagtatcag ggtaagagca
     6241 gcaattacaa caatcattag tttttgattt tttgtgttgt aaggataaag tataagcagg
     6301 agaatgcctg cggccaagca aaactccgaa aatgataata tgagatttat cggggtatcc
     6361 ggagagaaaa caaagattga gcagcacaat ccaaagggta tgaaaaacac ccagcccagc
     6421 agcttgaaaa gtgaaaccgt tttgttcatg cttatggtat ataatgcaat gaagaaagaa
     6481 aaacaagtca ttgcaattaa tagtatccta tcatatacac cctgatacat tacatgctac
     6541 cctttcatat ttatttcgac aattaatata tattatatca taaatttggg aattttatat
     6601 taatatgaaa gctaacctgc tgtaaattat gaaatgcatt aaagcagagg actgtatatt
     6661 cgcttctcca atattctgat tatatcatcc atagatttgt ttgttacatt caccaacaga
     6721 gccccgtatg atatattttc gtttgataac gccgcggttg agggaacatt attaactgca
     6781 tagtaaggac gtgtatccgc ctccttgtag aggactgcat cagccattct gttttcccct
     6841 atgtagaata cttcattccc tctgctttcc agttcttttt ttatatcttc caagccatgc
     6901 tgtattgcga ttatcataaa aacacctcca tagcaactta ttgtgcaata caattagtat
     6961 cctatgaagg tatatttttt attctatatt tgtgtacctt tcaggctcct gttcaatacc
     7021 agttcttctt caacagaaag aggatatctt gacgttcctt tgcttacagg cttggcataa
     7081 cctacaaact catttcttcc gtagatacct gtaattatga taccgtcatt aaaattgtca
     7141 agcatagcta tggaaaaact ctgatcgctt cccgtatcac taaaggcgtt gtatcttatt
     7201 atgttgcact tctgtatgca tttatccatc tggttcttta catgtattag gtcttcgctt
     7261 ataatgttca ctctttctat tgacgcttcc atattttcta tatgccccag cagcattcct
     7321 tcaaagttct tacctgaaga acccctcatg aatttcttgt atttttcact catcccggca
     7381 agcctgacag atagtataag attccagaca atcataataa acaataatat tgtaatagaa
     7441 aacagaattt cagcattgta tactttaata aaataatcca tgatattcat cccctactct
     7501 cctataacaa ggttccaatg ttttcaacag cattcagtgc cgcacctact tgttcttttg
     7561 tattgaaaaa accgaagctg aaccttacgg taccctgttc cagggtccct aatgttttat
     7621 gggccatggg cgcgcagtga agtcccgatc ttgttgctat atcgaatgtc cggtcaagaa
     7681 tatagcttat ttctgaagaa cccgtgtctc ctatgttgat tgatactaca ggggcctgct
     7741 tacttgcctc tttaacaccg tagaccttaa cgttctttat ctccgccagt ccatccaaaa
     7801 agtattttac aagctcctgt tcatgggctc ttattttatc aatcccctta ctctttatga
     7861 agcttaagcc cgcacacaag cctgcaatac ccggtgtgtt gtgagttccg ctttcaaagc
     7921 ggtcaggcat aatatcaggc tgatataatg attcggattt gcttccggtt cctccttcct
     7981 gcaaaggggt cagctccaag cctcttttta tacacaatcc gcctgtcccc tggggtccca
     8041 taaggctttt atgccccgca aaagccagca tgcttatatt catcttttgc atatctattt
     8101 catatacacc ggcagtctgt gaagcatcaa caagaaaagc caatccatac cttttcgcca
     8161 gatctccgat atcctcaatg ggtataatag ttcccacaac attcgaggca tgggcagcag
     8221 caataagtgc cgtttccttc tttatttcct tctcaatgct gccaatatca atttccccat
     8281 attcattgca ttgtgctatt gtcgtttcta taccgagttt ttcaagaacc ttcaaaggcc
     8341 tcattaccga attatgctcc atacttgtgg ttattacatg actgcccttt tttaagaagc
     8401 ctttgagagc cagattcaat gcctctgtgg cattatgagt caatataaca ttatcgggtg
     8461 aatctgcgtt aaacaactct gccagtaact ccctggcttc aagaagtacc ctgcccgatt
     8521 gaagggaaag cttgtgtccg cctcttcccg ggtttgcaca ataattcctg agacaatcat
     8581 ctacagccct gtataccgat tcgggctttg gaaacgtagt tgctgcatta tctaaatata
     8641 tcatagtttg ctcctttgtt tcacgtgaaa cattattaat atatatcgtt atagaagaat
     8701 gatacatcgc aattattaat tcgcagtcat actcccaaag caataacctt gtataccgac
     8761 tcgcacgata ttagctttgg acagaatcag agcttgatac ttgtaaaatc ctgacgcaaa
     8821 gggggacatg gggacgtttc tcctgtccca ctgagagcga gacacgcgaa acgtccccac
     8881 gtcctctgtg ctggatttac ctcacgtaaa tcccacggat tttactgcgt atcttctctc
     8941 gattctgttg cccaaagtta ataagtgctc gctgttatac aaggctattg ccctgaaagt
     9001 tgaatacaaa gcaagggctt gtatagtatg ataatattat ggaaatgcaa ataccgacgt
     9061 ggggaagata tcttcgacgg aactttccac ccggcctcgg aacgggaata ccccagagga
     9121 aaagctagtc tcacgacgct tttccgaccc tagggaaccc tatggttccc gaaggcgtta
     9181 gcaagcatcc tgagcaccct cctttatcgg cagggggctt ctcccctttg catcccccgc
     9241 tttggaaggt gaggagaaca tacttcccct acagtcggag tacttcacaa acccttgttt
     9301 tttagcattc ttattatatc acaacagatt ccatactatc tatcctggct ataccgccga
     9361 caaatatact ttcatttcct tgatcatact ttacaaaaat attgcttgga ctgttcatga
     9421 agtagccctg ttcaacctta taagttttgc cgcagtcaaa ttgcttatca tttctcttta
     9481 tataatatag caatgatgcg ttggaagtcc cggttgcgct ttcttcatct atacccaccg
     9541 ccggagcaaa gttccggcag caaaaatctg aatcctcttc aattgtttca cgtgaaacaa
     9601 catgcattcc tataatatcg tactctctgc agatttctat cattttatcc ttcttaattc
     9661 tcaatttctt caatgcctct ataccgttca cttccaatat aatatctttt agccccgtag
     9721 atataacatc aatcattccg tttcggtttg tagttatttc atccttttcc gcatcaataa
     9781 tatccattat tatattactg cttaactgca taatcagttc aggcttattc atttgaatca
     9841 atattgagtc cccttcgatt aataccttaa gctctcctgc ttttgtccga tgatatgcat
     9901 aattatctct cagatatcca agctgcttca gtacctgaaa ggaagcaagg gctgcatgtc
     9961 cgcaaatatc aacttcttct gccggtgtaa aatatctgat actaaacctg ttcccatcac
    10021 ttttattaaa atcaataaaa gcggtctctg aaaaattgtt tttcttcgcc agttccagca
    10081 tctgccaata tgacatccca aaatcatcaa aaacaactgc tgctttattg ccgcctcccc
    10141 tgtttgatac aaaggaactc aagatatact gctgcatata tcaaaacctc catataccca
    10201 ttaatgttat ctataattaa ttttttgatt tcataatata aaaccattat ggcacaaaaa
    10261 ataatcgatt ataaaccatt taaataaacc tttatttttc cagtaattca aaaattctat
    10321 caaattcttc atcactataa tactcaattt ctatactgcc cctatttttc ctatgatgta
    10381 tagtcacctt agtacccaat accgccctta atctttcttc caattgattg atttctatcc
    10441 gatatgcatc aggcaatact tcctttttct ttttctgctt tgtatccttg gccagcttct
    10501 caatctgtct gacatttaat tgctgggcga tgatcttttc tgccatatct aattgcttct
    10561 tttcccctga tattgaaagc aaagcccttg catgcccctg agatatcttt ccttccgcaa
    10621 tcatattctt gatttcctgg gggagctgaa gcagcctcag agaattgcca attgcaggcc
    10681 tgctctttcc tattttcttc gatatttcct cctgcgtcat gttatattct tcaataaggc
    10741 tcttataggc catagcctct tcaattgaat tcaaatcctc cctttgaaga ttctcaataa
    10801 gagcaagctc cattacctcc agattgctgc attcctttat aattgccggt atagtcttct
    10861 tcccggccag tcttgcagct cgccatcttc gctctcccgc aacaatctga taaccgtttc
    10921 cctgttttct tacaattata ggctgcacca caccatgctc tttaatagac tctgaaagat
    10981 ccgccagtgt cgcatcatta aatgcctttc ttggctgctt atcgttggct tctatatcag
    11041 taatttttat ttccactatt gagttccgat ctgactcttc accttcggga ataagcgcgc
    11101 caagtccttt acccaatcct ctcctgctaa tcatttaaat cacatcctca cttaattcta
    11161 tgaattcatc ggccaaatcc atatatgatt ccgcaccctt ggatttcata tcatattcaa
    11221 taattgacat tccatagctt ggtgcttccg caagccttac attcctagga atcattgtgg
    11281 tgtagacctt tcccctgaaa tactttttga cctcgtctac aacctggatc gaaaggtttg
    11341 tcctgccgtc gaacatgctc agtacaaccc cctctatttc caaatcggga ttaaggcttc
    11401 tcttgacaag ctttatggtg ttcatcagct ggcttacgcc ttccaaggca taatattcgc
    11461 attgtatagg tatcaatacg ctgtctactg cagaaagggc atttatcgat aacaaaccaa
    11521 gggacggggg acagtcaatg aatacataat catactcgtc ttttatactt tctatgcatt
    11581 cccgaagctt caattccctt ttgttcatct cggtcaattc aatctccgca cctgccagct
    11641 ggacacttga aggtatgatg aacagatttt ccctgtttgt gtcaataatg cattcccttg
    11701 ggtcgctgcc atttataagt acatcataaa ttgtcttttc cagcgttgtt ttatcaatcc
    11761 caaccccgct ggtggtatta ccctgggggt cgacatcaat catcagcact ttctttcccc
    11821 tgtttgccag gcatgcacac aaatttatga ccgttgtggt ttttcccacc ccgcccttct
    11881 gattaaagac agctattgat ttggacatag catacacctc tcattgtact attgtgttct
    11941 gatattatta taaagttata tagaaataaa tgtacatcct tttcttgaac ataaatgctt
    12001 acttccgaga ataaacatat tgtgaatata cataaattat caaagtatat gcatgctcac
    12061 ccggatcatt tagtatacag cacatattat tccatatata taatatatca gattaaaaaa
    12121 acaaatagaa tatgaatttg ccaaagactc tcccacaagt acaaataagg aggacaaaaa
    12181 tgttaaaatc cgtactttat acatttttcc ttgtctttat agccgagctg ggcgacaaga
    12241 cacagcttgc gactatgctg ctctctgcaa aatccaactc aataattgca gtattcatag
    12301 gcgcaagctt tgcattgata tgctcatctt ttatcggggt tttcgccgga gcttatataa
    12361 cgaagcacgt tcctcctcaa tatattcaga atgcggcagg ggttttgttc attgcaatgg
    12421 gtctgctgat actaaagggg aaaatataaa aaacgcgtct cacgacgcct tttttatatt
    12481 ttcccctttt tatacatttt tgggtatttt tactgtaatc tcaatgaatt ctcctttatc
    12541 aacctgaccg tattcaacct tgcagcccgc cgtcagaaac tccttgcagg catttttcag
    12601 ggtgtttaca taaatattat aattcatttt ccctttgatt cttttcttct taggctcctc
    12661 tgccgctgcc gccgcttcat ctagcatctt atcgaccagc tcttcagctt tttttacgtt
    12721 gagagacttt tttataatgc ttttgagagc tttctcctgc agtgtatcat ccggaagctt
    12781 cagcagcgcc cttgcatgcc tctctgtcag ttcattctcc agtattgtct ttttgatgct
    12841 gtccttgagc tttagaagcc tcagtttatt agctatggtg gattgctttt tgcccacctt
    12901 tctggcaagc tcttcctgag tcatcccgtg gtcatttatc aaattatgat agccctctgc
    12961 ttcttctatg aagtttaaat cttctctctg caggttctct atcagagcaa ttacagcaga
    13021 atcctgttca acaacctcta ttattactgc cggtatatga ctctttcccg caagctttga
    13081 tgatctaagc cttctttccc ccgagatcag ctcatatcct ttttccccta tttttcttac
    13141 attgataggc tgaagaacac cgtattcctt aattgaagaa gccaaatcct ccagggatga
    13201 tccgttgaat tcctttctag gctgatatgg gtttggagct atttcgtcaa tcggtatatt
    13261 aattatttct ttcctatcgt tcaaatcttt cattcctctc cataccatat gatttacttt
    13321 ttatattcgc tacaaacctt tgacttcctg cctgagaaag attgatttta ctcgttttca
    13381 gctgtttttt atataacaaa tttctggttt tttctgctat gttatcggat ttgatgccgg
    13441 tttacccggc tttctgggat attttgtcgg agttgctgaa accttctcaa taactattat
    13501 attgtgcctg atatcgctga atggcagttt atactccttt acctccttta ttgttcctcc
    13561 gagaattttg acagctgatg ccgcattacc gatctcctct tctatttcag ggcctttttg
    13621 acaaagaaag tatcctccga tcttcgcaaa aggaatgcaa tattcacata aaacgttcat
    13681 tgatgccact gctctggaca ggacaatatc atagcattcc ctgtaagctt tattgccccc
    13741 tgcttcctcc gcccttccgt gaacagcggt gatatctctt aaatcaagct tcttaattgc
    13801 ttcgctcaaa aatattaccc ttttgttaag ggaatccaga agtgtcattt tgagcccatc
    13861 catcaatatc ataacgggaa tcccggggaa acccgcaccg gtaccgacat ctattatgct
    13921 cataccttct tttatatatc ctgcagacag gcaggtagcc gaatcaagaa aatgtttgat
    13981 gaatatctcc ttttcctcag tgattgccgt aaggtttact ttttcattcc attccagcat
    14041 tatttcctta aaggctacaa gcttatccac ctttttattt gccggttcaa tccccagctc
    14101 cgttagtccg tcaatcagta ttttcctgtt ttccagcctg ctcaccccct cggccccttc
    14161 taatctgttc aagatatacc ataagcaccg atacatctgc cggagacacc ccggaaatcc
    14221 tggaggcctg gcccagattg gcaggattga tactgtcaag cttttgcctt gcttccagtg
    14281 aaagtccctt tatatctgcg aaatttatat tttccggtat tttcctgctt tccaattttt
    14341 taaattgctc tgcctgttgc agctgtttct taatatatcc ttcgtatttt atcattattt
    14401 ctatttcttc cgctgtctct ctgtctaact caggcatttc ttcgtcaatc tccttcagag
    14461 aacagtattc tatctccggc ctcttcaaaa gctcatacag gcttatgcct ccttttatct
    14521 ccgcgctttc tctgctcatt aagaattccc ttatatcctc tgtcattgaa atactcttct
    14581 tctttagcct gtctgtttct ttttctatat tattcttctt ttcaaggtat ctcttatatc
    14641 tctcctcaga tacaaggcct atgtcatatc ccttctgcgt aagcctgaga tctgcattgt
    14701 cctgccttag aataagcctg tactcggctc ttgaagtcat aagccggtat ggttcatttg
    14761 ttccttttgt aacaagatca tcaatcatta ctccaatata agcctccgac ctgtctaaaa
    14821 taaaaggctc ttttcctctg agacttaagg ccgcatttat tcccgctatt aatccctggg
    14881 ctgcggcttc ttcataaccg gaggtgccgt tgatctggcc ggcaaaaaat aaaccggata
    14941 ttttcttatg ctcaaggctt aattgcagct gggtaggatc aatgcaatca tattcaatag
    15001 cataagctgt tctcatcatg tggcagtttt ccagtccttt tacactgtgg agaatatcaa
    15061 gctgcacgtc ctccggcaag ctggaggaca tcccctgtac atacatctct ctggtattaa
    15121 gcccttcagg ttctataaat atctgatgct cctccctgtc agggaaggat actatcttcg
    15181 tctctattga gggacaatag cggggaccta ctcctacaat gtctcctgta taaaggggag
    15241 accggtggag gtttttccgt ataatatcat gagtcccttt gtttgtatat gtaagatagc
    15301 acgatagttg atcgatatct atgtccccgg tcataaagga aaatgtattt atatccctgt
    15361 ctccgggctg ttcagtcatc ctttcgaagt ccaccgtatt accgtcaatt ctggccggtg
    15421 ttcccgtttt gaatctcagc agctttattc ccagatcctc aaggctgtca gagagctttg
    15481 ccgccggaaa aagtccgtta ggacccccat catagcttat ctctccaatc actacttttc
    15541 ccttaaggta tactcctgta gccagaatca cagaccggca ttcaaatatt gctccaagat
    15601 gtgttacaac accttttacc ctgtcatttt ccacaataat cctcgtaatt tccgcctgtt
    15661 taatctgaag gttttcctga ttctccatca ctcttttcat tctatcctga tacagctttt
    15721 tatcaatctg tgctctcaat gctcttactg caggcccctt cctggtattc agcattctta
    15781 tctgaagcat tgttctgtct acatttattc ccatttctcc gcccaatgca tctatctccc
    15841 tcacaagatt gccttttgca gggcctccaa tagaaggatt acacgccagc attgctatac
    15901 tgtcgagatt tattgccgta accagggtct ttaatcccag ccttgcacat gccagggcag
    15961 cctcactgcc ggcatgtcct gcccctatga ctacaacatc atattttcct gctgtatatt
    16021 ccatatccac acctctattt gcctatacag aatttactga atatctcttt aatgatatct
    16081 tcctcaacgg tatcccctgt tatcgctccg aggctgttcc atgcttccct tatatcaacc
    16141 gatatcaagt caacaggaat attatcctct attgataata ttgccctttc tatattctca
    16201 agagctctgt ccagaaggtt cttatgcctg atatttgtaa ccatgctgtt tttgcttata
    16261 cttagcttcc catgatatac caggcccttt attctttcct ccagcttatc tatcccgatc
    16321 cggttcttta ccgacattct tattatattg tcctctccga aaacgcctct taaatacgcc
    16381 tcatcaataa caagccccaa gtctgtttta ttcaatacag ctattacttt tttatttttt
    16441 attaaatcta taatctcatt atcttcctgc cgaagctcat cggatgcgtc cagcaccaaa
    16501 ataatcaggt ccgaattact gatggcgcct tttgtcctct ccactccgat acgttcaact
    16561 atatcctcag agtctctgat acctgccgta tcaataagcc ttaccaatac tccccccata
    16621 ttaaggtatt cttcgattat atcccttgtt gttcccggta tctctgtcac tatggcacgg
    16681 ttctctttca gaagtacgtt cagaagagac gacttcccaa cattaggacg ccctataatt
    16741 gccgtgctta agccatccct gacgatttta ccctcgtcga aggactctgc aagctccata
    16801 acggaagcct ttgcttccag aagcatattc tttatgttag ccgtcgtaat ctcttccaca
    16861 tcatgttcgg ggaaatctat cgaagcttca atatgggcca gcatagaaag aagcttttcc
    16921 ataatcttct ttatctcttt tgacaactcc ccttccagtt ggttaagtgc ggcattcagc
    16981 ccggtttccg tcttcgctgt aataacatca ataactgctt ccgcctgcgc caaatctatt
    17041 cttccgttaa ggaaagcacg ttttgtaaat tcccccggct cagcaatatc ggcaccgttt
    17101 ctcaggactt cttccattat ccttctgatg ggtacaatgc ctccatgaca gtgcagctca
    17161 acaacgtcct ccatagtata tgaataaggc tttcgcataa caatcacaag cacttcgtcg
    17221 attatagcac ccttgctgtc aacagcatgg ccgtatacta ccgttcttga aggtatatca
    17281 tctatatcca taactctttt tggcttgaaa acttttttta atatctccgt ggatttctca
    17341 ccgcttattc tcacaatccc tatgccggct tcacccggag cagtagctat tgcagctata
    17401 gtcctttcaa acattatatc ctctccttag agtcagctgt actgttttta ttattgttta
    17461 caatgtttta aatatgtaca tataggttat tatatatcac tgtttaaatt ttggaaacaa
    17521 atttatatgt gagacatggg ggaccgggga catggggacg tttcgcgtgt cccgcttcca
    17581 gtgggacagg ggaaacgtcc ccatgtcccc atgtccccat gtctcacata taaaaaggga
    17641 tatcgctaga tctgcaacac cccgttcata ctttccaata atatttcagt tgaaattttc
    17701 tatttatttg atataataac ttttctgtac ggctcttccc cttcactaaa agttgttata
    17761 ccttcaactt cctgaagtgt agaatgtatt attcgtcttt catatggatt cataggctca
    17821 agctctatat ttttacctgt cttccgagcc ctgtcggcca gccttttggc aagtctctga
    17881 agcgtctcct ctcttttcct tctataatcc tcagtatcaa gtatgacttt tacgaagctg
    17941 tctctttcct tatccttgtt aacaaccaga cttacaagat attgaaggga atcaagtgtc
    18001 tgccctcttc tccctataat catacccatc tttggacctg aaagattgat atataagctt
    18061 tcgtttatat tctttactct tatttcagct tttatatcca tggcttccaa tacttctctc
    18121 aaaaattttc ttgctctttc cttctccagg gtatattcgg ttattcttat ttctttattt
    18181 tctatttcct ttttaacact tttctcggta gtttttttct ctttaagagt tactcttact
    18241 tttgcatttt tagagccaag gattccgaat attccctttg tcggctgttc caatatctct
    18301 atatccacat tatccttttc agtctctaat tcctctaatg cagattgtac agcttcttcc
    18361 actgttcttg ccgttttttc aaccatcatc atatctatta ttcctccttt ttagcagcct
    18421 gaggcttcgg cttcatcatg aaatactgct gtatgatcgt aaatatatta ccggcaaccc
    18481 aatacaaggc aagcccggaa ggaacattga gagtaatcca tccgaacatt atgggcatta
    18541 tatttgtcat catgctctgt gtctgagctg ctgtatcctc cttgcccttt tttttattgg
    18601 ctttaggagc acttatcatc ttcatctgat aatatgttgt cgctgctgat aatacagcca
    18661 gtataggcac tccaaatcct gcaactttaa tcattgtcgc ctgcatttcc ggtttaccca
    18721 ggtctaatag ccaccaaaaa ttcttcgcaa tcccggcgta tatattagca tccttaaata
    18781 catactttac cggattctgt atgacataaa acaatgcaaa aagaatgggc atttgaatga
    18841 gtgccggcag gcatcccgaa gcaggattat aattttgctc cttatacagc tgcagaagct
    18901 tttgattcat cttttccttg tcgttcttgt atttttcctg aatttccttc attttagggt
    18961 ttagttcatt cattttttta gtggattcag tctgcttgat attaataggg agcagtaata
    19021 ccttggttaa tattgtaaaa atgattattg agtatccgta atctcctaca aagtcaaata
    19081 tgaacttcaa caacgcacct aaaggttcgg caataaaatc taacaaatct tttccccctt
    19141 atatttcagt ttatagcaag atttcgaggc agaggatata tgtaaaccct tattggaatt
    19201 cttttcgcct cgttataatt aaactaacgg tttttcttct ccggtacggg atcataaccg
    19261 ccgggattaa acggattgca tcttaaaatt ctgcctgcgg acagatataa cccccgtatg
    19321 actccgtgcc tgtcaattgc ttcaattgca tagctcgagc aagtaggaat gaatctgcat
    19381 ttccctcttg ataatggaga tataagcaac tgatatgttt taataataac tataaggatt
    19441 tttctcattt attcctcgct ttttttaagc ttggcccttt tcaaaattga tattatatta
    19501 ttttcaatca tcgaataatc cgctgacccg cttcctgccc ttgctgtaaa aacaatatca
    19561 tagcctaatt ttacagtatc cgcaaacttt ctgtatgctt ccttcatcag ccttttgatt
    19621 ctatttctga ttacgctttt tccaaccttc ttgcttaccg agaaacccac tctattaaaa
    19681 ttggaatcat ttggaattat atacatgaca acaaattttt ccacataata ctttccttcg
    19741 ttataaactt ttttgaactc atagtttttc ttaagcttat gcaatgttgt catatcctgc
    19801 ctcttcccct tcatacagaa aaaggccaca gttcgcggcc ttatactgtc agtctttttc
    19861 taccttttct tcttcgcctt ctgaggactt ctctgcctgt agctgtgctc attctctccc
    19921 ggaagccatg aacttttttt ctttgtcttt tttttggctg atatgtcatt aacatgttat
    19981 gcacccccta atctctgagt atatcttaat tcaaagcaat tagcttaatg taataaagca
    20041 taattcacca ctttactatt aaaattatat aggcattgat agtttgtgtc aatacaatct
    20101 tttgctgcag cggacatagc tgcaccaagt gcaaataaag tattttcagt atctttgcct
    20161 ttatttaaga tattcttatt tgtattgaac ttgaccacct tttttgctat tattattata
    20221 tgatgtgtat aaccctgcat atttactgaa gttatcaaca actgtgcata atttgtgtat
    20281 aacctgtttg tgtgtattaa cttctatttt attgtcaata atctgataac tacgtttatt
    20341 tatattaaca acttatccag ataatattaa caatatttac agtatatatt aacaagttaa
    20401 tcacattttt attcatactt aaaaaattat accgcaattt tccatacact tgtctcggga
    20461 tataggagcg aagagaattt tcggttaagg aggcattcta tgtattctca attagttgaa
    20521 atctgggaaa aaactttaag cgtaattaag gttgaattga cagaagtaag ctttaataca
    20581 tggctcaaag ccatacaacc tgtttctata aaggaaaatc ttattacatt ctctgtgccg
    20641 gatgatttta caaaaggcat acttgaagca agatacagca agttgataaa aaatgcatta
    20701 aagcaagtta cttctgaata ttacgatata aggttgatca ttcccaccga agaaaatttt
    20761 acatcaataa tgacagaacg gtctgaaata aatcaatctg aggaatctgt caattcctcc
    20821 ttgaacccta aatatacttt tgaaaccttt gtaataggaa acagcaaccg tcttgctcat
    20881 gcagcctctg ttgctgtagc agaatcaccg gccaaagcat ataatcccct ctttatatat
    20941 ggaggcgtcg gattgggtaa aacccacctt atgcatgcaa taggccacta catacttaaa
    21001 cagaatatga gatccaaggt cgtctatatt tcttcagaaa cctttacaaa tgaacttatc
    21061 aattcaatca gagatgacag aaataatgaa ttcagaagca aatacagaaa tgtagatgtg
    21121 cttttaatag atgatataca gtttatagcc ggaaaagaaa gcacccagga ggaattcttc
    21181 cacaccttta attctttgta tgaggctaac aaacaaataa ttatatccag cgacagacct
    21241 ccgaaagaaa taagaacctt ggaagagcgg ctcagatcaa ggtttgaaca gggtttgata
    21301 gccgatatac aacctccgga tctggaaacc agaatcgcaa tattaagaaa aaaagcaaaa
    21361 tctgagaatc tttcgatatc tgatgacgtt atgctgctta ttgcaaaaaa aattcattca
    21421 aatataagag agcttgaagg agcattgata agggttgtgg catattcttc ccttacaaac
    21481 ggtgaaatta atgaagaact tgcaaatgaa gctttgaagg atatattatc aaactcaaaa
    21541 ccaaaaaagg ttactccttc agcgataaaa gatgttgtgg gaaattatta caatataaaa
    21601 attgaggatt tcaatgcgaa aaaaagaaac aaatccgtag tattgccaag acaaattgca
    21661 atgttcctct gcagagagct tacagacctt tccttaccgc ggataggtga agaattcggc
    21721 agagaccata caacaataat gcatgcatac gaaaaaatat cctccgatat aaaaattgac
    21781 actcagttaa aatcagtttt ggaggagttg aaaagtaaaa tagaaggtta atatcctgtt
    21841 gatatactat taataacctg tttataagtt ttttgattat tttagttata ctttgctatc
    21901 aacagaattc cttcgtaaac tccacagatt ataaacacgc acattatttc ttaaagcagg
    21961 atttttgggg cttttgcact tattaacagc ccctactact attactacta aaattattct
    22021 tttatattat ataaaaataa atatcggagg ttatccacaa tgaaaataat aactacaaaa
    22081 tctacattat tggaatccat aaatgtagta caaaaagcag ttccttcaaa aaccactctt
    22141 cctatattgg aaggaattct ttttgatgca aaaagcggca agctcaagct gacggcaaca
    22201 gatctcgaaa tgggaataga aacaacatca aacgttgatg ttattgaacc aggcaaaatt
    22261 gtgctgtctt caagaatggt aggagaaata gtcagaaaac ttcctgattc ggatgtagaa
    22321 atagaaacgg cagaaggaaa tttagtcagc attaaaagcg aaggctcaaa attcaaaatt
    22381 gttactcttc cttatgatga atatcccgag cttccggttg tgaaaaagga gaacggaatt
    22441 actattaaac aaaacattct gaaggatatg ataagaaaga cgatatttgc agtatccact
    22501 gacgaaatca ggccgatact tacaggagta ctttttgatg tatcgggtga agagcttacg
    22561 atggtggctc tcgatggttt cagaatggcg gtaaagaaaa atgttattat gaatgataat
    22621 aattttaaag ccgttattcc gggaaagaca ctgtctgaga taggaaaaat aataaacgat
    22681 aatgaagaaa acataaatat ttatttttca aagaaccaca ttcaagttca aattgaggat
    22741 actattataa tatcgagact tttagaggga gaatttataa attataaaca gataatacct
    22801 gcagaatata aaataaaaac aaaggtgtct acaaacctgc ttatggaggc ttgcgacagg
    22861 gcagctttat ttgcaagaga ctccagcaac aacatgataa aatttgaaat aaacgatgat
    22921 atgatggtca taatgtcaaa ttcgcagaac ggtgatgtac acgaggaact taaaatacaa
    22981 aaagaaggaa gcgacataga aattgcattt aatgcaaagt atttcataga cgtgcttaaa
    23041 gtaatagaaa gcgaagaaat aacaatagaa tttactacaa acgtaagccc aagcataata
    23101 agacctgtta aggatgagga atatttatat ctggttcttc ctgcaaggtt caaaaagaac
    23161 agctaaatta aaagggctgc tgcaaaacta tatttcgcag cagcccttta gttaaaaaag
    23221 aagagagtga tattatgaga gaagcaaaaa tagaaacaga ttttattaag cttgatcagt
    23281 tcctgaagtt tgaaaatatt gcaggaaccg gcggagaagc aaagaacatg attatggacg
    23341 gattagtaaa agttaacgga aatatagaaa aagcaagggg taaaaagctc cgcaggggag
    23401 atgttataga aatttttggc gaagaaatca agcttatcta ataaattata ggggtgctta
    23461 tattgtatat aaaaaggatt gatttgaaga atttcagaaa ctataaagag tttgatattg
    23521 agctgggcaa aggagtaaat atattttacg gtcagaacgc acaaggaaaa accaatataa
    23581 ttgaatccat atatatggca tctaccggca aatctcaccg gacgcaaaaa gacatggagc
    23641 ttatcagatg gaacagtgaa gacgctaaaa taagaataga ttatcaaaaa aaagaagaag
    23701 aaaaaataga aatatattta aataaaagtc taaagaaaca aattaaaaag aacggagtaa
    23761 ggctgtttaa aataggagag ttaataggaa atcttaatac agttatattc tctccagatc
    23821 atatgaaaat aataaaggaa gggcctgctg aaagaaggag gtttattgac ataatactat
    23881 cacaggtaaa accgggttat tattataatc tggttcagta tctcaggact cttgaacaga
    23941 gaaataacct gctggaagaa gcaagaacaa attcaggcat tcttaagacc atagacatat
    24001 ggaatgaaca actagctgat tttggtgcca ggttaataaa agcgaggcaa gatttcatat
    24061 gtgacatatg ctgcatggca gctgatattc acaaaaaaat atccaacgga aaagaaacac
    24121 tggttatcaa atataattct tcaataaagt atcatagtaa ttcggaaaag gaaataaaag
    24181 agaattttat cattgcgctg gaaaaccata ttaatgccga tatgggaaga agcgctacac
    24241 acaaaggacc ccacagggat gatatattat tttttatcaa tggaacagag gtaaagacat
    24301 attcatctca gggccaacag aggacaaccc ttctctcatt aaaaatagca gaactcaaat
    24361 atatgaaaga agagacggga gaattaccgg tacttttgtt ggatgacgtt ttttcagagc
    24421 ttgatgatga gcggcaaaaa tacttgattg agtttattaa agacatgcag gcaatcatta
    24481 cctgtaccaa tgtagaacat cttgaaaagc ttatgcttga agggtctcgg acatataaag
    24541 tggaaagcgg gatgatagta aaagattagc ttttagtttt ttacagaaaa ggttataata
    24601 atatggtatg aacaagataa gttaattagg atgcaaggga ggacaagttg atgtttatgc
    24661 atctaggagg agatgttgta atatctctga aggatataat ttcaatcatg gatatagaat
    24721 ccagcagtac atccagtatt acaaaagagt ttttaaagac tgcagaggat gagggcttta
    24781 taagaagaat atcagaggat gaagaaaagt cttttatact gacggaaaag aacaaaaaga
    24841 caataatata tttgtctccc atttcttctg taaccttatt taaaagagca ggattcataa
    24901 aagacatatc gctgaagtag agggggaata ggaatggaag aaaataagtt tgaaggcaca
    24961 tatgaagcgg aacagattca agtacttgaa ggactggaag ctgttaaaaa aagacccgga
    25021 atgtatatag gcagcaccgg atccagaggc cttcatcatt tggtttggga ggtagtggac
    25081 aacagcattg atgaagcatt ggtaggcgcc tgtaaaaata taaaagtaac catacatcct
    25141 gataattcaa tcagtaatga agatgacgga agaggttttc cggtaggaat gcatccaaag
    25201 cttaacaaac ccgcagtaga agtagctctt acagttcttc atgcgggaag taaattcgga
    25261 ggaggaggct ataaggtttc cggcggtttg cacggtgtcg gagtatccgt agtcaatgct
    25321 ttatccgaat ggcttgaggt tgaggttaaa agagatggga agatatacca ccagcgatat
    25381 gaaaggggct ttcccgttac cgaattaaaa gtcatcggta cgacaaacag aacaggaaca
    25441 agagtgtcct tcaagcctga cagtgatgta tttgatgaac tggaatacag ctttgatata
    25501 ttggaacaca ggcttagaga gttggcattt cttaacaaag gaataaagat aagcctcaaa
    25561 gatgaaaggg acggaacaga aaagacattt caatatgaag gcggaataat atcttttgtt
    25621 aagcatctta ataagaacag agaggttctt cataaggagc cgatatattt tgaagcaaaa
    25681 aaagacaatg ttgatgttga aatagcgctt cagtacaatg acggttatgc ggagagtatt
    25741 ttcagctttg cgaacaacat taacacatta gagggcggaa gccatatgag tggcttcaag
    25801 aacgctgtaa ccaaagtgat aaatgattat gccagaaaat ataaatttct taaagaaaat
    25861 gatgccaatc ttataggtga agatgtaaga gaaggcatgt catgcgtaat cagcatcagg
    25921 cttaccgatc cccagtttga gggacagacc aagacaaagc ttggaaaccc ggaggtcaga
    25981 ggaatagttg acagtattgt tacagagaat tttgaagcct ttttggagga aaccccttca
    26041 aacgcaaaaa taattgttga gaaagcactg actgcagcaa gggctaggga tgcagcgaaa
    26101 aaggcaagag agctgacaag gaggaaaaat gtactggaaa gcactacact ccccggtaaa
    26161 ttggcagact gctctgagag ggatcccagc ctttgtgaaa tatatatagt tgaaggagat
    26221 tctgcgggag gttctgcaaa acaggggaga gacagaaggt atcaggcaat acttccattg
    26281 agaggtaaaa tacttaacgt agaaaaagca cggcttgaca gaatattgaa cagcgagatg
    26341 ataagagcaa tgataacagc ttttggggcc ggtataagcg atgatttcaa tttagaaaaa
    26401 atgagatacg gcaaaataat aatgatgact gatgccgatg tagacggtgc acacataaga
    26461 acattgctcc tgacattttt ctataggtac atgagtcctt tgatagaagc cggatatgtt
    26521 tatattgcac aaccgccgct ttacaaagta cggaagaaga ataaagacta ttatgcatat
    26581 aatgacaaag agcttgaaaa agtgttcaag gagataggca gagagggttc cagcatacag
    26641 aggtacaagg gtcttggaga gatgaaccct gagcagctgt gggatactac aatgaatcct
    26701 gaaacaagga tcatacttca ggtaacaatg gaggatgctg tggcggccga tgagatattt
    26761 acagtactaa tgggagataa agtggagccc agaagagagt ttattgaaca taatgctaag
    26821 tatgtaagga atcttgatat ttaagataag ctttggaggt aattgatttg gattatgaga
    26881 atcaaaagat actgcccata aatattgaag aagaaatgaa aaaatcattc atagattacg
    26941 caatgagtgt tatagttcaa cgtgcattgc ctgatgtaag agatggtttg aagcctgtcc
    27001 acagaagaat tctatatgca atgaatgact tgggaattac tcccgataaa cctcatagaa
    27061 agtgtgcaat gactgtgggt gaagttttag gtaaatacca tccccatggt gactcttctg
    27121 tttatgatgc aatggttcga atggctcagg acttttctca aagatatatg ctcattgacg
    27181 gccatggaaa cttcggttcc gtagacggag acggtgctgc ggctatgcgt tatacggaag
    27241 ccagaatgtc aaaaatagct atggaactgc tgaaggaaat agataaggaa acagttgatt
    27301 ttacaccaaa ctatgatgaa agccttaaag aaccgactgt tcttccggct cgttttccga
    27361 atttactggt aaacgggagc aatggaatag ctgttggtat ggcaacctcc atacctccgc
    27421 ataatctcgg agaggttatt gactgtgcag tatcaaccat tgaaaatcct gacatagaat
    27481 tggaagagat gcttaaatat ataaaaggcc ctgattttcc taccggcgct ataattatgg
    27541 gcaaggaagg aataaaaaaa gcttatagta ccgggagagg aaaggttatc gtaagggcta
    27601 aagcagaaat tgaggaaatg agcggaaaca ggcaaagaat tattgtcagt gagataccct
    27661 accaggtcaa taaagccata atgatagaga aaattgcaga tctcgttaaa gaaaagaaaa
    27721 ttgacggtat ctcagactta agggatgaat ccgacagaaa cggtatgagg gttgttatag
    27781 aactcaagcg agatgcaaat gccaatataa tactgaatca gctttataag catacccaaa
    27841 tgcaggaaac cttcagtata attatgattg cccttgttaa cggacaacca aaggttctga
    27901 accttaagag cgttatcact cattatatag accaccagaa agacgttgta acgagaagaa
    27961 ctcaatatga tcttaaaaaa gccgaagcca gggcacatat attggaaggc ttgagaatag
    28021 ctcttgataa tattgatgag atagtaagca tactaagaaa ctccaaaaat gttgctgaag
    28081 ccaaggaaag aatgatgagc agattcggcc tttcagaaat acaggcggaa gcaatagccg
    28141 aaatgaaact taagagctta accggcttgg aaagaggcaa gattgaggaa gaatatcttt
    28201 caataataaa gaatattgaa tatttcagat cacttcttgc taatgaacag aagcttttaa
    28261 tggttataaa agacgaactt ttggaaataa agaaaaaatt taatgacaag agaagaacaa
    28321 aaattattcc tacatcatta gaggaatttg atgaagagga tttcatagaa gagcatgaag
    28381 tagctgttac aattactcat ttcggatata taaaaagact tcctgcggac acctataaga
    28441 gtcagagaag aggaggccgc ggaataacag gacttcagac cagagaagac gattttgtag
    28501 aaaatctgtt tataacctct acacataatt atattttgtt ctttacaaac aaaggaaaaa
    28561 tgtataagct taagacctat gagattcctg aggcaggcag acaggccaaa ggaacagctt
    28621 tggtcaatct tctgcagctg gatgcagaag aaaaagtaaa tgcggttatt catataaagg
    28681 attttaatga cggcaaatat cttattttca tgacaaagca cggtacaata aaaaagaccg
    28741 aactgaaaga atatgatact tcaagaaata cagggattac agctataaac ctcattgata
    28801 acaatgaact aatcggggtc aaactaaccc gtgggacaac ggagttgata gcggctacaa
    28861 aaaaaggact tgcaataaga tttccggaaa gcgatgtaag gcctatggga agaagtgcca
    28921 ggggagtacg tggaataaac ttaaatcgcg atgatgttgt gataggaatg gatattgtag
    28981 aggaaggagc cgaccttctt gtaattagtg aaaaaggtta cggcaaacgt actccgatag
    29041 atgaatacag agttcagaca aggggcggca aaggaataat aacatcaaga ataaccccca
    29101 aaactggaca tgttgtggga atgaagatcg tgaaagagca agacgaaata atgttaatca
    29161 atttatcggg cattattata agaatgaatg tcaacgaaat atcaaaaatg ggaagaagca
    29221 ctcaaggcgt taaactgatg cgggttcctg agaacgaaag cgtagtatca atagcaaaaa
    29281 taagcagcaa tgaggaagaa gagaagtaac ggcgattctt taaggacagt catgcataac
    29341 cttaacagtt tgtataaggt tatgcatgat tttttattgt gaggataaat cctccacctc
    29401 attgaaatct tgtaataaca cggaaaaagc agaaaaatat caagattcga actattaata
    29461 aataatagta gggtaaaagg tttataatat taaaggaatg ttatctgata agcatagaaa
    29521 aaattagtag ttgacgctta tcatatatgt cccttccgga cagcgatgct gtctgtcagc
    29581 aatgaagcaa aagcttcatt gaatggacat aatcaaggta tgttatatga taaacttatg
    29641 gaatgaatta cttattgacg tttatcatat a
//
LOCUS       DAOQUR010000029        28967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_1909_length_28967_cov_11.282340, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000029 DAOQUR010000000
VERSION     DAOQUR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 28967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28967
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1909_length_28967_cov_11.282340"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PKI50_07530"
     CDS             complement(<1..790)
                     /locus_tag="PKI50_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT03588.1"
                     /translation="MGRSRISRGELHYFSDSLKQKLKDILHALVTVVEAPSGYGKTTA
                     IRDFLKAELSQGTPVYWFTAVDEAPAAGFRRLCHEIDKIDSQAGERLLKIEFPNAVTA
                     GEACDAIRSIQCRHEAYLVIDNFQLLHTFLPPSFFTALLEHGGEGLHIIIVTQLLKRN
                     MLTAIAGRGFLHITASDLKLDAEDIRRYYALAGLRITHEDALAVEHYTEGWIIAVYLQ
                     LCAYLDTGVLYGMSGILALMEHLVWDTLTEEQQVFLLRLSPFEMI"
     gene            complement(1055..1396)
                     /locus_tag="PKI50_07535"
     CDS             complement(1055..1396)
                     /locus_tag="PKI50_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03589.1"
                     /translation="MEHVYDSQSRLQGYVDGDVIYDGWNRVAGYTDGRMIYDEYSNPM
                     AYADDRFVYGMDGRPIGFYSNDYRLYDMSGRYMGYGNQGFRGLLGTVLLLLLLRRFRR
                     RRRRRRFGFGF"
     gene            1800..4457
                     /locus_tag="PKI50_07540"
     CDS             1800..4457
                     /locus_tag="PKI50_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNT03590.1"
                     /translation="MWFSKSPEEVLREFDVSQSVGLSGEEALKRLEKFGENKLKGKPK
                     KSLISLFLGQLRDMLIYILLAASVITILIGEYVDAVIILMVVILNAVIGVIQEYKAEK
                     AIEALQKMTTPKALVRRDGEVKEINSEELVPGDIVVIDAGRYIPADLRLLGSVNLQIE
                     ESALTGESVPVDKNANALFDNPKAPLGDLSNMAFMSTLSTYGRGEGVVVATAMETEIG
                     KIAKILDEDSDEMTPLQKRLDELGRILGFLCIGICALIFIVALIQKRELFEMFLTAIS
                     LAVAAIPEGLAAIVAIVLAIGVTRMSKINAIVKKLPAVETLGCVNIICSDKTGTLTQN
                     KMTVVKHYTLNNLKELPAAQTAFEAVGDEAELIKSFVLCSDATYENGKGTGDPTEIAL
                     VVMGDKYNLSKTSLNEKFERVGEKPFDSDRKLMSTLNKEGNGYRVHTKGALDNILKIS
                     ASALVNGKIVPLTDGMKADYMKVAEEMSDEALRVLGAAFKDTGCIITPEEMEKDLTVI
                     GFVGMIDPPRLEVKDSIREAKTAGITSIMITGDHKNTAVAIAKELGIAESREQSMTGS
                     EIDELSVEEFAERIGSYRVFARVSPEHKVKIVKAFKSHGNIVSMTGDGVNDAPSLKYA
                     DIGVAMGITGTDVAKGASDMILTDDNFTTIVHAIEEGRNIYNNIKKSVIFLLSCNLGE
                     VISIFASILFFWPIPLLPTQILWMNLITDTLPAIALGVDPGDKDVMKKKPRDSKESFF
                     ARGAGVKAITGGSLIGILTLAAFYFGLHEFGYSLGSGEIPEEVLTYARTMSFVVIVAS
                     QLFFSLSMRNATKSIFQIGFFTNMKLIGAIIAGFILQFGVITIPVFARAFKVHTLSLY
                     DWSLVIAFALVPFLVNELLKVFMKAVYKE"
     gene            4712..4930
                     /locus_tag="PKI50_07545"
     CDS             4712..4930
                     /locus_tag="PKI50_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03591.1"
                     /translation="MVQDSGPLIELDSMLNEFNSEKLPFVGDRSGPIGKFTNTKAVLK
                     ENVHITLPNALSTIAPGKKASCSTLMLC"
     gene            5327..5548
                     /locus_tag="PKI50_07550"
     CDS             5327..5548
                     /locus_tag="PKI50_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03592.1"
                     /translation="MQSHRIEDTGTIDFKAFILYFENTFLETIAEELFGNRNLVLKNN
                     CFELSPELRELIYIHPRMPCKAAGLFHDA"
     gene            5538..5744
                     /locus_tag="PKI50_07555"
     CDS             5538..5744
                     /locus_tag="PKI50_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT03593.1"
                     /translation="MMLDLKIEKAKDLLQSTGFSIEQICDLCGFSSLSYFSQIFKNKT
                     SLTPTQFKGQYNHSSKLFFFKGKE"
     gene            complement(5761..6201)
                     /locus_tag="PKI50_07560"
     CDS             complement(5761..6201)
                     /locus_tag="PKI50_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03594.1"
                     /translation="MRKIIDERIIAGKRKINSSAFGICFLALWGILLYRQFMLHQDIR
                     EYADIFLLTVGISIYVVVSNILQGFYYTYRSRSTQKKTLLIGALTGTIVFSTVQILIM
                     DYDLANGKDVSKLVLQAIIFLAVWIACQHALFKISEKQADKDIE"
     gene            complement(6205..6405)
                     /locus_tag="PKI50_07565"
     CDS             complement(6205..6405)
                     /locus_tag="PKI50_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT03595.1"
                     /translation="MGDKNNKLRLARIERDLTQEQLADKIGVTRQTIGLIEAGKYNPT
                     IKLCIAICKELNKTLDDIFWED"
     gene            complement(6455..7339)
                     /locus_tag="PKI50_07570"
     CDS             complement(6455..7339)
                     /locus_tag="PKI50_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT03596.1"
                     /translation="MRVLLPIAYAHRKMAGIPLKGWLSFAQAIERRFIELGGSIQYQS
                     KVERLIIENGAVKGIILADGSRHFADRVLSAVDGRFSQSLLLGKPEGDTKQTFNPNAV
                     SDQPVQVNIGVDMDFSAEEGPVTYILPDSFNAAGREHGRITVHNKCFDPDAAPKGKSA
                     ITVFLDSDYLWWKDFASDKARYKREKDRCAEAVIDIMDRYRPGFKDKIEVIDVTTPLT
                     RERYTGNWMGAMQAWKPDSSIIRALLQSRPRYAYKDIQGLYMAGQWVEAWGGITTAAQ
                     SGRKAVQAMCRHDGKCKI"
     gene            complement(7507..8445)
                     /locus_tag="PKI50_07575"
     CDS             complement(7507..8445)
                     /locus_tag="PKI50_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HNT03597.1"
                     /translation="MLSNFEYIRESLELNLFFMRIAKEHSIFIEAAFTAKNREPAEEA
                     EAFKIVFSNLLIKAIELSDGILSEDVTGSGELVTDLTLEAEKETEFYTGISINSRITK
                     AELAFANEEEPSIDPVLLQDVYMLNQQALASTRALANYKARLLRDVLACRLFTNNYPL
                     LLDHILREAKFYIRSLMRLQRKNSSNTIDDAIKEEIFWNRIMAEHSKFIRGLLDPTEV
                     KLFDTANDFGNRFDMLTAEAEKLEDNLNLFAKVTTETSDAAKDIRDFKRQGTQGLINC
                     TIRSIAYPLLGDHVVREANHYLRLLKKYASMPVSSR"
     gene            complement(8664..9755)
                     /locus_tag="PKI50_07580"
     CDS             complement(8664..9755)
                     /locus_tag="PKI50_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNT03598.1"
                     /translation="MNSKIKCGAVIAAAGMSSRMNDFKPLLPLAGTTLIGRVINTLKS
                     AGVSPIVVISGKFAERLKEYLTGFDVICLYNGNYERTDMFYSACMGFEYIQDKAEQFF
                     FLPGDVPLFSKQSLDAMMEHMSISGCDILLPQRCGRQGHPILIKSSVVPAVLSYDGEN
                     GLRGVIENYKGPKEAMELPDIGITLDADSPRDYELIKLLADDTVPEDIRRHSALVAQL
                     AVSLGEKLISLGYSLDLHRIELAALLHDISRAQPNHACAGAEHLEKIGHPLLAEIVSQ
                     HMKLLPEQEDKISEVTIVYLADKFFIGGEIATLEQRFENKRREFHDKPGALQALEENY
                     QTSLGILRLFENALGFSIVPNELLKKQTV"
     gene            complement(9757..10068)
                     /locus_tag="PKI50_07585"
     CDS             complement(9757..10068)
                     /locus_tag="PKI50_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNT03599.1"
                     /translation="MEFKNATIVKKANIYFDGKVSSRTIFTANGERKTLGVMLPGEYE
                     FGTNAEELMEVLGGEMDVCLPGEKVFKTYKEGDSFIVPANSKFKLILKTVADYCCSYK
                     E"
     gene            complement(10110..11345)
                     /locus_tag="PKI50_07590"
     CDS             complement(10110..11345)
                     /locus_tag="PKI50_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HNT03600.1"
                     /translation="MTLISPVQQWAAERTGLKEKLSLQTLMAWQLDKVRKAVEYARNN
                     CDYYCKKLKGIDISGIKTLRDMQDIPFTYPEDINEDPKAFVCVPTREITRITSLSTSG
                     SQGSPKRIYFSENDLERTIDFFACGMSTMVHSGQSVLIMMSSRTENSIADLLEKALKR
                     IGVSSDIHGNVKDAAAALEAAKGYDCLVGVPAEIMYLCRTDDRLRPESVLLSADYVPE
                     SVIRYLEETWHCKVFTHYGMTETGFGGGVQCEAGEGYHLRDADLLIEIVDSITGKQLN
                     AGKLGEVVLTTLRNEAMPLIRYRTGDIARILTKPCPCGGILPRLDKVRGRSANIIRLE
                     NGKSINIHDLDEIMFAIPGLRNYSARLVGRNRLLLTADTKSQLDAELLREKLPCDIKV
                     EYGTVVPFTGAGKRNICLE"
     gene            complement(11342..11977)
                     /locus_tag="PKI50_07595"
     CDS             complement(11342..11977)
                     /locus_tag="PKI50_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT03601.1"
                     /translation="MKNICTGDICRPGGYKITERALEFCGFSKDAKLADIGCGLGATV
                     RHIRRNYDYDICGIEKDKDAVKLAVTQFVYAGDSTELPFDSEELDGLLFECSFSKMDN
                     PELVLTECQRVLKPGGYLIISDMYARGSSAALSGLLGRIEAKEEILDKLIMSGFDIKL
                     FEDYSDELHRMWGQLIFEHGAKALCENLGTDSGKLKEIKCGYCLIIAQKGA"
     gene            complement(11974..13284)
                     /locus_tag="PKI50_07600"
     CDS             complement(11974..13284)
                     /locus_tag="PKI50_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT03602.1"
                     /translation="MSIPKIIRSTYSVCPVCLSRIPAQHVEIGSNVYLQKKCVQHGFF
                     SSVIWRSKTPIGEWIGDTPRIKKNENLNCPKACGICPEHRQGTCCVLLEVTSRCNLRC
                     KYCFADSRNTDDPSLKQVQAWLRQLAVPGKTLVQLSGGEPTVRDDLPEIISSAKEAGC
                     RYVQLNSNGIRLAEDKEFVRKLAEAGLSFVFMQFDGTNDEINRKLRGVDLLALKKQAI
                     ENCAAYNIGVTLVPTMVPGVNTDNIGELLRFAIAESPAVRGVHFQPVSYFGRIPERPV
                     DEVRYTLDELIYDIEVQTKGQIKAKNLIPSRCDHPLCGFHGDFVVMPHGIEPLSGKYT
                     SKGDCCGSEPVSADKNRAFVARRWERQVQEQESHSCCSKPDIHDMDYFLKRVKSHGFT
                     ITAMAFQDAGNMDLERLRRCSLHVFDNGRFVPFCAYYLTGWDMT"
     gene            complement(13285..13479)
                     /locus_tag="PKI50_07605"
     CDS             complement(13285..13479)
                     /locus_tag="PKI50_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03603.1"
                     /translation="MADNKVIICCKCKKELIPKKVYFKYLGHSFFTDILKCPDCGEVF
                     IPEELVKGRMAEVEMQLEDK"
     gene            complement(13483..15732)
                     /locus_tag="PKI50_07610"
     CDS             complement(13483..15732)
                     /locus_tag="PKI50_07610"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT03604.1"
                     /translation="MVLEMEKFKEKIKTCLQNEPAFCTAACPFRLDIHDFMPKMQRGG
                     FNAAYRTYLNTVGFPGIVSELCHEPCKNVCPRRLKDEAIAMKHLEKAAIKYARDLNPN
                     SYNLPPKNKKIAIIGAGISGLGCALRLASKKYNVTVFERTDRIGGHLWDVLPPEVFLA
                     DIERQFMHEKYTLNLNTEITSLDELDFDAIYVATGKGGTDFGLKPDPEGAFASDKPGV
                     FMGGELCGRNIIEALADGLQAINAIERYIKTGNMNQPKAKDKTEILINTDLISYMKPV
                     LPDEGEVFTQENALYEAKRCLRCSCDACVRHCDLMSYYGKYPKRIGEEVEITVNPGTL
                     DGNGTVATRLISTCNQCGLCKGICPQEIDTGDFLLQSHRAMRGKGAMPWAFHDFWLRD
                     MEFTNSEAAGMCKLPKGFDKSRYVFFPGCQLGASDPWYVIESYRFLREKTPDTALILG
                     CCGAPAEWAGDEQLHEAAVAKLREDWISLGKPIAVFACPTCRQMFRKYLPEIEGIFLY
                     NLISELGIKPSPRVNGDTVSVFDPCTTREEPELQHVIREIAEKAGFNLEPLPYEGKYA
                     KCCSWGGQVSIANPSFTREVVKARISQNSNPYITYCINCRDIFDAAKKPVYHILDIIF
                     GSHDKDQMLPTITQRRSNRINLKHKLLSEFWRDEAIMEQKESRIKLLITPELRQKLHK
                     EMILETDIETVIEHCESTGRKVQIPESGHFFGHLQIGNMTYWVEYLPEDGKFNLINAY
                     CHRMSIEEA"
     gene            complement(15844..18765)
                     /locus_tag="PKI50_07615"
     CDS             complement(15844..18765)
                     /locus_tag="PKI50_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT03605.1"
                     /translation="MKLRKMWLNVNGANRMFACNPEKDTLASVLRRMGLTGVKVGCGT
                     GVCGACSVILNGKVIRSCTKKISTVEEYSKVITIEGIGTPNNLHPLQVAWMNYGAVQC
                     GFCTPGFIVSAYALLQENNNPTREEVRDWFQKHRNVCRCTGYKQLVDAVMAAAKVVRG
                     EATIEDISFKLPEDKEYYGKPVVRPTALAKVCGLADYGNDQELKMPAETLHVAATQPR
                     VAHHAKILNIDTSEAEKMPGVVKIITHKELKEAGGTNVMAEAQFHERTTVMVPSRKVL
                     CEDKIFRYGDVVALAVADTKEHARAAAAKVKVEIEKLPEYLSYLDAVMPDAMRVHEDT
                     PNIFSMQPVLKGVGLEDAAKVQEVIDNSAYSVSGSFYSTREPHLTIEGTTVQAYFDEE
                     DNLTIQCKSQGVYSSKGRMGDSVGIPKDKLRIVMNPTGASFGWSTNAGDICLAAAAAV
                     VTKMPVALSMSYEEHQHFSGKRCPCHSNGRAACDENGKITAVEFDFGMDHGAYSWGGD
                     DLMTKQVRFAFFPYKVPNAAGLTRIANTNHNFGTAYRAYGSPQAYTLSEAMMDMLAKK
                     AGIDPFEFRWINIAREGDTNINSYPFRQYPMEEIMKIMRPYYEKAVAEAKAADTPQKR
                     RGVGLSWGGFNVTEGGTDSSSVALELNPDNTFTKYDTYQELGQGGDIGSLMLTLEALK
                     PLGVKPDQIRLVQNDTKLCPDSGMSGASRTHFMSGKATILAAEKLMNAMRKPDGTYRT
                     YDEMAAEGIPTKYQATYSNTTIPGICRLDPNTGIGDPTPAYTYCLNLAEVEVDTATGK
                     VRVLRFTCVDDVGKIGNIDAVNGQAYGGISHCIGFALSENYDDVEKHSNMLGAGVPYI
                     KDIPDEINVIHYERPDEVGPFGSSGASEAFQSSGHVAVLNAIDNACGVRIYEMPATPE
                     KVKAGLDILAAGGKIEPPKKYFLGSDLYDELENIKANPVKYGGDDFFIPLGDATAERF
                     F"
     gene            19359..19799
                     /locus_tag="PKI50_07620"
     CDS             19359..19799
                     /locus_tag="PKI50_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNT03606.1"
                     /translation="MENIMTLEKVNELRSQGFNCSQIVFLYGSERLGLEPEKALKIAG
                     AFGGGMYNGETCGCVTGALMVLGLKYGQSVPYDLAAKNLLIQKKTEFEEKFKEAHETL
                     ICKEILGYDISNPSEKSIIVEKGLLDEICPYLIWDACQILDEML"
     gene            complement(19928..20830)
                     /locus_tag="PKI50_07625"
     CDS             complement(19928..20830)
                     /locus_tag="PKI50_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT03607.1"
                     /translation="MNRIKLVLVMIIWGSLGVFTKSIPLSALSLAFLRASIALPVLFV
                     VMKMKKADKLKWTLLKPYLISGILLGFGWLTLFYGFKNTSIASAVIIYNMSPVYVMIF
                     APLALKETISKIQVAVILTSFLGLFLIVGHNLLEGYGYMGMLLSAVSGMIYASIVLIN
                     RSIKVRVDNQTATFVQIFTAMLVLLPFVLIEGNLLTAVHLDAKAAIYTILLGVLHTGV
                     AYTMFFSLYTNMKSVEIISYSYLEPLFGILFSVIFMGETLIFTQIIGGILILGSTFIG
                     EMLKNRGYLEKKIHSNLSANVTDR"
     gene            complement(20902..21681)
                     /locus_tag="PKI50_07630"
     CDS             complement(20902..21681)
                     /locus_tag="PKI50_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT03608.1"
                     /translation="MSDKSRMEFSRSIIEGVEIKLCRNDPHAYKPHVHNELSLGYIVE
                     GTTDLTLNGSTIHYGPGDGVIIPPLMTHRCAPNDINHWAYVMLFVDPGYYGDLVTFNQ
                     AKKLTGNQLQRLVGFIEQLLTEKIPNTLENILIELLIEFGEDDIPETAAANTIDNIKK
                     IHDYILNHVSDDITLDKLQQISGLNKFSIIRNFKKLYLTTPAAYHLQYRVAEAKRLLS
                     KGADVFEICVELKFYDQAHLIREFKKMYGVTPGAYIKQLMG"
     gene            complement(21929..25360)
                     /locus_tag="PKI50_07635"
     CDS             complement(21929..25360)
                     /locus_tag="PKI50_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNT03609.1"
                     /translation="MLTDNAVKVLEKRYLIKDENGKPVETPEHMFRRVASTIAAADSF
                     YGSPADVAETEEKFYKLMSSLEFIPNSPTLMNAGRELGQLSACFVLPVEDSMEGIFDA
                     IKNAAMIHKSGGGTGFSFSRLRKKGSAVATTGGVASGPVSFMKVFNAATEAVKQGGTR
                     RGANMGILRVDHPDIIEFIQCKKDNSDITNFNISVAITEKFMEAAEKGEDYELIDPAT
                     GKPAGKLNAKEVYDLIVDMAWNNGEPGIIFLDRINRDNAVPRLGEIESTNPCITGDSW
                     VLTRKGPVQVKDILGRQVQLALNGSYSMSDEKGFFSTGKKSIIRVCTDRGYELKLTKD
                     HLIRTAANMTRFKIDEEWKAAGDLRPGDRIIMSNNRSIRWEGKGSFDEGYLLGVLIGD
                     GTLKKEGGIISVWGSDEEAVSLMKAAGEAACALPHRSDFRGFQTVISDREENRMRLSA
                     LRDLALEYGVMPGDKKIASEVEKGSYEFYRGFLRGMFDTDGTVAGTQEKGLSVRLWQN
                     DLDTLKAVQRMLGRMGIISAIYSNRKEPGIKSMPDGKGGLKDYNVKAGHELVISKDNL
                     YVFHELIGFSSSKKQNRLKSGLGNYKRALNRERFVASVTEIIESGEAEVFDVQVPGIN
                     AFDANGIYIHNCGEQPLLPYESCNLGSINLMSCIKKKNKFGKVIDYEKLGSIVDESVH
                     FLDNVIDVNKYPLPMVDEMTKGTRKIGLGVMGFADMLVELGIPYNSEEAVNTAESIMK
                     FINERSKAASMKLAEKRGTFKYYEDSVFKEKGIRIRNATTTTIAPTGTISIIAGVSSG
                     IEPLFALSFVRNVMDNDELPEVNPLFERVARERGFYSTELMKKIAAHGTIQDIKSIPK
                     DIRKVFVTAHDIEPEWHIRIQSAFQKYTDNAVSKTVNFSNSAARDDVSAVYMLAYRMG
                     CKGVTIYRDGSRESQVLNIGSANKDNKEKPAPANEVLVPRTRPEVTTGVTEKVKIGCG
                     NLYITVNSDENGICEVFTNLGRAGGCPSQSEATSRLISIALRAGVDIGEIIEQLRGIR
                     CHSTLRQKGLKVLSCPDAIGRALERYMNTKVDNKDIKTMDELAMEIEERGNCINNCSL
                     CAEPCHAVTVEEGSISYNAYGSFNGNMKNCPECGSTIQHEGGCVICRNCGYSKCN"
     gene            complement(25619..26788)
                     /locus_tag="PKI50_07640"
     CDS             complement(25619..26788)
                     /locus_tag="PKI50_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNT03610.1"
                     /translation="MMRKWTIQAAVILLGIMFQMNAANAERYIYGKNEIDLSYEKDCY
                     LQQEGPDWELMKKRLKVKGIYMTGYTVAMTERFNKLIDLVKTTELNAVVIDVKDDNGL
                     MTYSSDLPDVDFTGANKKVRIKDIDAVMKILRGNNIYSIARIVTFKDRIAGDKYANLA
                     VKNTSGGIWRDRHGMSWLNPYNRESWDYLVDIAEEAAAKGFNEIQFDYVRFPTDGNTK
                     IIDYGTSAAGKSKAEAIAEFLSYGRERLSKMGVVVSADVFGLVTTATDDMRIGQHLES
                     IAKSVDVICPMVYPSHYGKGSYGVAEPDFEPYKIVNRSMSVAQERINAMKDVRRKAVL
                     RPWLQDFTASYLDRYKRYGPAEVRAQIDATYDAGQEEWLLWNAGNVFTSGALLKE"
     gene            complement(26973..28634)
                     /gene="ilvD"
                     /locus_tag="PKI50_07645"
     CDS             complement(26973..28634)
                     /gene="ilvD"
                     /locus_tag="PKI50_07645"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNT03611.1"
                     /translation="MRSDQMKAGIERIPHRALLKGLGLTDEEIRRPMIGIVNSQNDVI
                     PGHLHLDDIMEAVKAGVRMAGGTPFQFPAIGVCDGLAMGHEGMKYSLISREHIADSVE
                     IMAMAHPFDALVFIPNCDKIVPGMIMAALRLNIPSVFVSGGPMMPAFLDGKKLTLSST
                     FEAVGAAGANKMNEQEILDYENYSCPGCGSCAGMFTANSMNCMTEAIGLGLPGNGTIP
                     AVNAARVRLAKLAGMKVMEMLEKNIRPRDIVTADSLHNALTADMALGCSSNTVLHLPA
                     IAHEAGLKIDLKDINDISKATPHLVKLAPAGPNAMSDLWQAGGVQAVLKELEGMGLLK
                     TDCITVTGCTAAENLKDVTVKDREIIRTKETAYSQDGGLAILWGNLAPDGAVVKKGAV
                     LPEMMVHRGPAKVYNSEEDCMAAIMGGKVVPGDVVVVRYEGPKGGPGMREMLSPTSSI
                     AGMGLDSSVALITDGRFSGASRGASIGHISPEAAAGGLIGIVEDGDIISIDIPNNKLE
                     LLVDEKTIAERKTKFVPFEKPVPNGYLKRYRRMVTSANTGAVFED"
BASE COUNT         7764 a         6991 c         5637 g         8575 t
ORIGIN      
        1 taatcatctc aaagggagac agccgcagaa gaaatacctg ctgctcttcg gtcagggtat
       61 cccatacaag atgctccata agagccagta taccggacat accgtataaa actcccgtat
      121 ccagataagc acagagctgc aaatatactg ctatgatcca gccctcagtg taatgctcaa
      181 cagccagggc atcctcatga gtgattctta aacccgcaag ggcatagtaa cgtcttatat
      241 cctctgcatc cagcttcagg tcggaggcag tgatatgaag gaatccgcgg cctgcaattg
      301 ccgtaagcat attgcgttta agcagctggg tcacaattat gatatgcagc ccttctcccc
      361 cgtgttccaa aagtgcagta aaaaaagaag ggggcaggaa tgtatgcagt aattggaagt
      421 tgtctatcac aaggtaggct tcatgtctgc attgtatgct tcggattgca tcacaagcct
      481 cccctgcggt aacggcattg ggaaattcaa tttttaacag tcgttctcct gcctggctgt
      541 ctattttgtc aatttcatgg caaagacgac ggaatcctgc ggcaggagct tcatcaacag
      601 cagtaaacca ataaacaggc gtaccctgag aaagctccgc tttcaagaaa tcccttattg
      661 ccgtggtttt gccataaccg gagggagctt ccacaacagt aaccagcgca tgaagaatgt
      721 cctttaattt ttgcttcagg ctgtcggaga aataatgcag ttctcctctg ctgattctgc
      781 ttcttcccat atactccctc ctttgatggc tattgtaatt attatacaaa tattatgaaa
      841 aaatggcaat atactgcaaa gttttgcaca atagaagaat aatgcaacag aggacacggg
      901 gacgcgggga cgcggggacg cggggacgtt tcgcgtgtcc cactggaagt gggacagggg
      961 aaacgtcccc gtgtccccgt gtccccatgt cctcagaaag caagcgggga atttcttttg
     1021 tagcttttct gtctcaatgt aaaaatgcaa agagttaaaa cccgaatccg aatctgcgtc
     1081 ttcgtcttcg ccttctgaag cgtctgagaa gcagcaggag gagcacagta ccaagcaatc
     1141 ctctgaagcc ttgatttcca tagcccatat acctgcctga catatcgtat agtctgtagt
     1201 cgttactata aaaccctata ggcctgccat ccattccgta aacaaacctg tcgtctgcat
     1261 atgccatagg gttggaatat tcatcgtaaa tcatccggcc atctgtataa cctgcaacac
     1321 gattccagcc gtcataaatc acatcaccgt caacgtatcc ctgaagcctg ctttgtgaat
     1381 catatacatg ctccaaaatc tcaccccctt ttttatttcg tttatataaa ttaaagaaac
     1441 ttcattcccc tatcattatt atgtagcatg tacattcctt gacatcatca aattgcatat
     1501 tgtatcaaaa taccggatat ggtattctat aatagaagta agttgtgtga atgttatctg
     1561 ttaaagggga gtaactgcct gataaaggtg atttaagtca acaaccggta gttggcacac
     1621 tgcctggctt aatcctttga cgattcaaag agtaagacct ttagcataat tttatccgta
     1681 atgataaaat tttgctaaag gtctttttta aacacagcaa gagagtgaca ccccgcactc
     1741 ttttataaag aatgctgatt atatttaata atatttatac gaacggagcg tgaagcaaaa
     1801 tgtggttttc aaaatcaccg gaagaagtac tgagggaatt tgatgtcagc cagtccgtcg
     1861 gcctttcagg tgaagaagct ttgaaaaggc tggaaaagtt cggtgagaac aagttaaagg
     1921 ggaaaccgaa aaaaagcctg atttccttgt ttctgggaca gcttagggac atgctgatat
     1981 acatacttct ggcagcatca gtaattacca ttctcatagg cgaatatgta gatgccgtta
     2041 tcattcttat ggttgtcata ttgaatgcag taataggagt tatacaggaa tataaggccg
     2101 aaaaagcaat tgaagcactt cagaagatga ctactcccaa agcccttgtc aggcgtgacg
     2161 gtgaagtgaa agaaatcaac tcagaagaac tggttcccgg cgatatagtc gttattgacg
     2221 ccggaagata tattcctgct gacctaaggc ttctcggaag cgtaaatctg caaatagaag
     2281 aatcagcact tacaggagag tctgttcctg tagataagaa tgcaaatgct ttatttgaca
     2341 atccaaaggc tcccttgggt gatttgtcga acatggcatt catgtccaca ctgtctactt
     2401 acggcagagg tgagggtgtt gtcgttgcta cggccatgga aacagagata ggaaaaatag
     2461 caaaaatact tgacgaagac agcgatgaaa tgacacctct ccagaaaagg cttgatgaac
     2521 tgggaagaat acttggtttt ttgtgcatag gaatatgtgc ccttatattc atagttgcgc
     2581 ttatacaaaa aagagaactt tttgagatgt tcctgacggc aataagcctt gcagttgctg
     2641 caattccgga aggcctggcg gcaatagtgg ccatagtgct ggctattggg gttacacgga
     2701 tgtccaaaat aaatgccata gtcaaaaaac tcccggcagt ggaaactctc ggttgtgtta
     2761 atataatctg ctccgataaa acaggaactc ttactcaaaa caaaatgacg gttgttaaac
     2821 actataccct gaataatttg aaggaattac ccgccgccca gactgctttc gaagcagttg
     2881 gggatgaggc ggaattgatc aaatcctttg ttttatgttc ggatgccacc tatgaaaacg
     2941 gcaagggtac aggagaccct accgaaatag ccctggttgt aatgggtgat aaatataacc
     3001 tgtcgaagac aagcttgaat gaaaaatttg aaagagtggg agaaaagccc ttcgactcgg
     3061 acagaaaact gatgtcaacc ttgaataagg aaggaaacgg ttacagagtg catacaaaag
     3121 gtgcattgga taatatactg aaaatatcag caagtgccct ggtaaacgga aagatagtac
     3181 cattaaccga tggaatgaaa gccgattaca tgaaagtcgc cgaagaaatg tccgatgagg
     3241 ctttaagagt acttggagcg gcatttaaag ataccggctg tataataaca cctgaggaaa
     3301 tggaaaaaga cttgacggta ataggctttg tgggaatgat agatcctccg aggctggaag
     3361 tcaaggattc cataagggaa gcaaaaactg ccggcattac atcgataatg ataaccggag
     3421 accataaaaa taccgcggtt gcaattgcaa aggaactggg gattgccgaa tcaagagagc
     3481 aaagcatgac aggctcggaa atcgatgaac tgtcagttga ggaatttgca gagagaatag
     3541 gcagctacag agtatttgca agagtatcgc cggaacataa ggtaaagata gtcaaagcat
     3601 tcaaatccca cggcaacatt gtatccatga ccggtgacgg cgttaatgac gctccttctc
     3661 tgaaatatgc ggatatcggt gttgcaatgg gcataaccgg aactgacgtg gccaagggtg
     3721 ccagtgacat gatattgact gatgacaact tcacgaccat agtccacgca attgaagaag
     3781 gcagaaatat ttataacaat atcaaaaaat ccgtaatatt cctattgtcc tgcaatcttg
     3841 gagaagttat ttcaatattc gcatccatat tgttcttctg gccgataccg ttactgccaa
     3901 cccagattct ctggatgaat cttattaccg atacattacc ggctattgca cttggcgtcg
     3961 atcccggaga taaggatgtc atgaagaaaa aacccagaga ttccaaagaa agcttctttg
     4021 caagaggcgc aggcgtaaaa gcaattaccg gcggttcact cattggtata cttactttgg
     4081 cagcctttta tttcgggctc catgaattcg gatacagcct gggttcaggt gaaatacccg
     4141 aagaagtact gacttatgcc aggacaatgt cctttgttgt aattgttgct tcacagctgt
     4201 tcttctctct ttcaatgagg aatgcaacga aatccatatt ccaaattgga ttcttcacaa
     4261 atatgaagct gataggtgca attattgcag gttttatcct gcagttcgga gtaataacaa
     4321 taccggtttt cgcaagggca tttaaagtcc acaccttaag cttgtatgat tggagcctgg
     4381 taattgcatt tgctctggtt ccgttcctgg taaacgagtt attgaaagta ttcatgaaag
     4441 ctgtttataa agagtaatag ctgaaaaact cttctgacac ctgacttcgg tcaggtgttt
     4501 ttttgcttat accgcaatat atcataaata aatcagcaat ctgaataata tctattggtc
     4561 tggcaggttc tatactgtta ttagaaaata tccgattaaa ctgtatagtt ggaggtgtaa
     4621 atgtatacaa gcaagcaaaa actcttgttt cggtgcttct catctttgct ttgatgttcg
     4681 cgccgatact gctgccccca atcaggcagc cttggttcag gatagcgggc ccctcataga
     4741 gcttgacagc atgcttaatg agtttaactc agaaaaactg ccttttgtag gggacagaag
     4801 cgggcccata ggaaagttta cgaatacaaa ggcagtgctt aaggaaaatg ttcatataac
     4861 tttacccaat gcattgagta ctatagcacc ggggaagaag gcatcctgtt caaccctgat
     4921 gctgtgctga ccatagaagg agcaagcaag atcgtccata atgcagtgtc aatccttctg
     4981 ggtaatgctc aacaatatga taaaaaagaa ggaagcggga aatagtctgc ttcttaatat
     5041 tgcccgataa gcatgagatg agtttattga tataatcttt tgtaagagag tgagattatg
     5101 gaagactttt tgctggcact aaaaccaact tttcctgatg tgcaaatgaa taagtcccaa
     5161 gtatatccat gcacaaatct ggcggtcata gaaacctcct atataatgtt ggaggaatgc
     5221 gcatgcaatc aattccatta tataatttcc atgaaacagt cacccttggt cagaatggag
     5281 aataaactgc atatattaaa aaaaaaacgt tttcccatgc aatcctatgc agagccatcg
     5341 aatagaagat acgggcacta tcgatttcaa ggcttttatt ctctatttcg aaaatacatt
     5401 tcttgaaaca atcgcggaag agctgttcgg caatagaaat ctggtgctga aaaataattg
     5461 ttttgaactc agtccggagc ttagggagtt gatatatata catccgcgaa tgccgtgcaa
     5521 agcagccggg ctgttccatg atgcttgatt tgaaaattga gaaagcaaaa gacttgctgc
     5581 aaagtacagg tttttcaata gagcaaattt gtgatttatg cggcttcagc agtttgagct
     5641 atttctctca gattttcaag aataaaacaa gccttacccc tacacagttt aaaggccaat
     5701 ataatcattc atccaagctg ttcttcttta aagggaaaga ataacggttt ttgttccgga
     5761 ctactcaata tccttatccg cctgcttttc acttatttta aataaagcat gctggcaggc
     5821 aatccatact gccaagaaaa taattgcctg aagtacgagc tttgatacat cctttccatt
     5881 agccagatca tagtccatta tcagtatctg aactgttgaa aatactatgg ttcctgtcaa
     5941 tgcacctatt aataaggttt ttttctgtgt gcttctgctc ctgtaggtat agtaaaaccc
     6001 ctgcagtata ttgctgacaa caacatatat ggaaatgccg acagtaagaa ggaatatatc
     6061 ggcatattcc ctgatatcct gatgaagcat gaactgcctg taaagcagta tgccccataa
     6121 tgccagaaag cagattccga atgcacttga gtttatcttc cttttgccgg ctatgattct
     6181 ttcatctata atctttctca taaactaatc ctcccagaaa atatcgtcta aagtcttgtt
     6241 cagctctttg catattgcta tgcacagttt tattgtagga ttgtacttgc cggcttcgat
     6301 aagacctata gtttgcctgg taacccctat cttatctgcc agctgctcct gcgtcagatc
     6361 tctctctatt cttgccagtc tcagcttatt gtttttatct cccaacgtat caccctctta
     6421 ccgcgattgt aacatatata ttgcttaatg tcaactatat tttgcattta ccatcatgcc
     6481 tgcacattgc ctgtacagct tttctgcctg attgagcagc tgtagtaata cctccccagg
     6541 cttccaccca ttgccccgcc atatacagac cttgtatgtc cttataagca tatctcggcc
     6601 tgctttgtaa aagagccctt ataatacttg aatccggttt ccaggcctgc atggcaccca
     6661 tccagttccc ggtataacgc tctcgtgtaa ggggtgttgt gacatctatc acttctatct
     6721 tatctttaaa gccggggcgg taacgatcca ttatatcgat aacagcttca gcacagcgat
     6781 ctttctctct tttatagcgg gctttatccg aagcaaaatc tttccaccaa agataatccg
     6841 aatccaaaaa aactgtaatg gcactcttac ctttaggggc tgcatccgga tcaaagcatt
     6901 tattgtgtac agtgattcta ccgtgctccc gaccagctgc attgaaactg tcaggcagta
     6961 tatatgtaac aggcccttct tcagcggaaa aatccatgtc aacacctata ttaacttgta
     7021 ccggctgatc cgaaacagca tttggattaa aggtttgttt tgtatctccc tcaggctttc
     7081 caagcagtaa cgactgagaa aagcggccat caacagcaga aaggaccctg tccgcaaaat
     7141 gtctggagcc atctgcaaga ataatacctt ttacggcccc attttctata ataagccttt
     7201 ccacctttga ttgatattgt atacttccac ccagttctat gaaccttctt tctatggctt
     7261 gtgcaaagga aagccatcct ttaaggggta ttcctgccat tttcctatgt gcatacgcaa
     7321 taggaagcag aacccgcaag cccggctaca ctcccatcaa ataggtaccc ttttctttcc
     7381 catgatgtac atagtcctcc cggtttatcc cccatttcaa agatggttgc ccgatacccg
     7441 ttcattcttg cataacaccc ggaagtaagg ccggcaactc cgcctccaat aattaattat
     7501 ctgcatttat ctggatgata caggcatact tgcatatttc ttaagaagcc tcaaataatg
     7561 gtttgcctct ctgactacat gatcgcccaa aagcggatag gcaatcgatc tgatagtaca
     7621 gttaatcaaa ccttgtgtcc cttgcctttt aaaatccctg atgtcttttg cagcatccga
     7681 ggtttccgtt gttaccttcg cgaacaagtt taaattatct tccagtttct ctgcctcggc
     7741 agtcaacata tcaaatctat tgccaaaatc gtttgcagta tcaaaaagct tcacttctgt
     7801 tgggtccagc aggcctctta tgaattttga atgctccgcc attattctgt tccagaatat
     7861 ctcctcttta atagcatcat ctattgtatt tgatgaattt ttccgctgca atcgcatcaa
     7921 gcttcttata taaaattttg cttctctcag aatatgatcc agaagcaatg ggtaattatt
     7981 agtaaatagt ctgcaggcta aaacatcgcg gagcaacctt gctttgtaat ttgccaatgc
     8041 gcgggtcgaa gctaaggctt gctggttcag catataaaca tcctgcagca aaaccggatc
     8101 aatactgggt tcctcctcat ttgcgaaggc taattccgct ttggtaattc tgctgttaat
     8161 acttatccct gtatagaatt cggtttcctt ctccgcctcc agggttaggt ccgtaaccaa
     8221 ttccccggaa ccggttacat cttcactaag tataccatcc gaaagctcaa ttgcctttat
     8281 cagaagattg ctgaaaacaa ttttgaaagc ctcagcctct tctgccggtt ctctattttt
     8341 tgcagtaaaa gccgcttcta taaatattga gtgctctttt gctattctca taaaaaataa
     8401 gtttaactcc agtgattctc ttatatattc aaaattagac agcatatatt accaactcct
     8461 ttaaaaataa gtattcacga tatatatatt catgcgacgc gcgcattagt aatgaactca
     8521 atataattta aaaggcaggc actttagagt acctacctta aattggttaa tatatgcagt
     8581 tttttagtac agataacaat attgccgcat taactgtgtc ttctttcttc ttgattgatt
     8641 cttaagtttt tgaagtgact ctttcataca gtttgtttct ttaaaagttc attaggaact
     8701 atactaaatc ctaaagcatt ttcaaaaagc cgcagtatgc ctaatgaagt ttggtagttt
     8761 tcttccaacg cctgcaatgc cccgggttta tcgtgaaatt ccctccgctt gttctcaaag
     8821 cgctgctcca gagttgcaat ttctccgcct ataaaaaatt tatccgcaag gtatacaata
     8881 gtaacctcag atattttgtc ttcctgctcc ggtaacagct tcatgtgctg actcacaatt
     8941 tcggcaagaa gaggatgccc gattttttcc aagtgctcag ctccggcaca tgcatgattt
     9001 ggctgcgccc ttgatatatc gtgaagcaag gcggcaagct caatccggtg caaatcaagt
     9061 gagtatccga ggctgattag tttttctccc aagcttactg ccaactgtgc aaccaaagcc
     9121 gagtgtctgc ggatatcctc cggcacggta tcatctgcca gcagctttat tagctcatag
     9181 tcccgggggc tgtctgcgtc cagtgttatc ccaatgtccg gaagctccat tgcttctttt
     9241 ggccccttat aattttcgat aacccccctc aagccgttct ccccgtcata agaaagaacg
     9301 gcaggaacaa cactgctttt aattaatatt ggatgtccct gccttccgca tctttgcggc
     9361 aataagatat cgcagccgct tatactcata tgttccatca ttgcgtcaag gctttgcttc
     9421 gaaaaaagcg gtacgtcccc ggggagaaaa aagaattgtt cggccttgtc ctgaatatac
     9481 tcaaaaccca tacaagccga ataaaacata tctgtacgct catagttccc attataaagg
     9541 catatcacat caaatccggt caaatattcc ttcaaacgtt ccgcaaactt gcctgatatg
     9601 acaacaattg gtgatactcc tgccgatttc agggtattga ttaccctgcc gattaatgtt
     9661 gtaccggcaa gcggaagcag cggcttaaag tcattcatcc ttgaggacat tcccgccgct
     9721 gctatcaccg ctccgcattt tattttacta ttcaaactac tctttgtatg agcagcaata
     9781 atccgccact gtctttaaaa tcagtttgaa tttggagttg gcaggaacaa taaagctatc
     9841 gccttcttta taggttttga atactttttc accgggcagg caaacatcca tctcaccgcc
     9901 aagaacctcc ataagttcct cagcgtttgt gccgaattca tattcgccgg ggagcatgac
     9961 ccccagagtc ttcctctctc cgtttgcggt aaatattgtt ctgctgctta ccttcccgtc
    10021 gaaatagata ttggcctttt ttacgatagt cgcatttttg aattccattt tttaccctcc
    10081 tttaattagt tattattttt atattaatac tattccaggc aaatgttgcg tttccctgcg
    10141 cctgtaaaag gaacaacagt gccgtattcc acctttatat cacagggcag tttttccctc
    10201 aaaagctcag catccaattg ggattttgtg tctgctgtaa ggagaagtct gttgcggccc
    10261 acaagccttg cgctgtaatt cctgagaccg ggaattgcaa acatgatttc gtccaaatca
    10321 tgaatgttga ttgatttccc attctccaga cggatgatat tggcgctgcg ccccctgacc
    10381 ttgtccaacc gcggtaatat acccccgcac gggcatggtt ttgtcagtat tcttgcgata
    10441 tctcccgtgc ggtatctgat taaaggcata gcttcgtttc gcagtgtagt aagtaccact
    10501 tcccccaatt ttcccgcatt caactgcttg cccgttatgc tgtctacaat ttcaataagc
    10561 aggtctgcat cccttaaatg atatccttca cccgcctcac actgcacacc gccgccgaat
    10621 cccgtttctg tcatgccgta atgagtaaac actttgcaat gccatgtttc ttcaaggtat
    10681 ctaatgacgc tctccggtac atagtctgca cttaacagta cactttcagg ccggagtctg
    10741 tcatctgtac ggcacagata catgatttcc gccggtacac ctacaaggca gtcgtaacct
    10801 ttcgccgcct ccaatgctgc cgctgcgtcc tttacattgc cgtgaatatc agaggagact
    10861 ccgatccgtt tcagggcttt ctcaagcaaa tcggcaatac tgttctccgt ccggctggac
    10921 atcattatta aaacagattg cccgctgtga accatggttg acatgccgca ggcgaagaaa
    10981 tctattgtcc gctccaggtc attttcacta aaatatatcc ttttggggct tccctggctg
    11041 ccagaagtgg aaagtgaggt aatccgcgta atctcccggg ttggtacgca aacaaaggct
    11101 tttgggtctt cattgatatc ttcaggataa gtaaagggta tatcctgcat atcccgtaaa
    11161 gttttgatac cggatatatc gattcccttc aacttcttgc aataataatc gcagttatta
    11221 cgggcatatt caacggcttt tcttacctta tccaattgcc aggccatcag tgtctgcagc
    11281 gaaagctttt ccttcaggcc ggtgcgttcc gctgcccatt gctgaaccgg tgaaatcaaa
    11341 gtcatgcccc cttttgagct attataaggc aatagccgca ttttatttct ttaagcttcc
    11401 cgctgtcagt ccccaggttt tcacataatg cttttgcgcc atgttcaaaa atcaactgcc
    11461 cccacatcct gtgaagctca tcactgtagt cttcaaaaag cttaatatca aacccgctca
    11521 ttatgagctt atccaggatt tcctcttttg cttctatgcg gcccaacaga cccgaaagcg
    11581 ctgcacttga acctctggca tacatatcgg atattattag ataccctccg ggcttcaata
    11641 ctcgctgaca ctcagtcaaa acaagctccg gattatccat ttttgagaag ctgcactcaa
    11701 aaagaagccc atcaagttcc tcgctgtcaa aaggcaattc cgtactgtcc ccggcataaa
    11761 caaattgagt caccgccagc ttcactgcat ccttatcctt ttcgattccg cagatatcat
    11821 agtcgtaatt ccgtcggata tgccgcactg tggcacccag cccgcagcct atatccgcaa
    11881 gctttgcatc cttcgaaaaa ccacaaaatt ccaaggctct ttctgttatt ttatagcccc
    11941 cggggcggca gatatcaccg gtacaaatat tcttcatgtc atatcccatc cagtcaggta
    12001 atatgcgcaa aaaggcacga atcttccatt atcaaaaaca tgcaggctgc acctgcgcag
    12061 ccgttccaaa tccatattcc cggcatcctg gaatgccatg gctgtaattg tgaagccatg
    12121 ggattttaca cgcttcagga aataatccat atcatgaata tccggcttgc tgcagcagga
    12181 atgcgattcc tgctcctgca cctgcctctc ccagcgcctt gctacaaaag ccctgttttt
    12241 atcggcggat accggttcgc ttccgcagca gtcccccttg gaggtatatt taccggaaag
    12301 cggctcaatt ccatgaggca taaccacaaa gtccccatga aagccgcaaa gggggtggtc
    12361 gcacctggag ggtatcaggt tttttgcttt gatctgcccc tttgtctgca cttcaatatc
    12421 atatataagc tcatcaagag tgtatcttac ctcatccaca ggcctttcgg gtattcgtcc
    12481 gaaataactt accggctgaa aatgaacacc tctgacagca ggtgattctg caatggcaaa
    12541 acgcagcagt tcccctatgt tatcggtatt gacaccggga accatagtcg gtacaagggt
    12601 cacacctata ttgtaagctg cacagttctc aatggcctgt tttttcaggg caagtaaatc
    12661 cacccctcga agctttctgt ttatttcatc gttggtcccg tcaaactgca taaatacaaa
    12721 agataacccg gcctcagcca gctttcttac aaattcttta tcttcagcca accgtattcc
    12781 gttgctgtta agctgcacat acctgcagcc cgcctccttg gcactgctta tgatttcggg
    12841 aagatcatcc cttacagtag gctcaccgcc gctgagctgt accagcgttt tgcccggtac
    12901 tgccagctgc cgcagccagg cctgcacttg cttcaagctt ggatcatctg tattcctgct
    12961 gtctgcaaaa cagtatttac atctcagatt gcagcggctt gtcacctcca ggagaacaca
    13021 gcatgtcccc tgcctatgtt cggggcagat accgcaagcc tttgggcaat tgaggttttc
    13081 gttcttcttt attcttggtg tgtcacctat ccactcaccg ataggagtct tggagcgcca
    13141 tattacactg gaaaagaagc cgtgctgtac acacttcttc tgtaaataaa cattactgcc
    13201 aatttcaaca tgctgcgccg ggatgcggct cagacaaacc gggcatacgc tataggtgct
    13261 tcgtattatt ttcggtatac tcatctattt gtcctccagc tgcatctcaa cctcagccat
    13321 gcgccccttg accagctctt cgggaataaa aacctcaccg caatccggac atttaagtat
    13381 gtcggtgaaa aagctgtggc ccaaatactt gaaatatact tttttcggta tcagttcctt
    13441 tttgcactta cagcatatga taaccttatt atccgccatt tgtcaggcct cctctatgct
    13501 catccggtga cagtatgcat taatcagatt gaatttcccg tcctcaggaa ggtattctac
    13561 ccaataggtc atatttccta tctgcagatg cccgaaaaaa tggccggatt ccggaatctg
    13621 aaccttccgc ccggtgcttt cacaatgctc gatcaccgtc tcaatatctg tttccagtat
    13681 catttcctta tgcagctttt gtctgagttc cggtgttata agcagcttta tcctgctttc
    13741 cttttgttcc ataatagctt catccctcca aaactcgctg agcagcttgt gcttaaggtt
    13801 tatgcggttg cttctgcgct gggtaatggt tggcagcatt tgatccttat catgggatcc
    13861 aaatattata tctaatatat gatatacggg ctttttcgct gcgtcaaaaa tatcacggca
    13921 attaatgcaa tatgtgatat aaggattgct gttctgagag attctggcct taacaacctc
    13981 ccgggtgaag gatgggttcg cgattgacac ctgcccgccc cagctgcagc acttggcgta
    14041 ctttccctcg taaggaagcg gttcaaggtt gaaaccggcc ttttccgcta tctctcgtat
    14101 tacatgctga agctccggct cttccctggt ggtgcaaggg tcaaaaaccg atacggtatc
    14161 tccgttgact cttggagatg gtttaatgcc taattcagaa attaagttgt atagaaatat
    14221 cccctcgatt tccggcagat atttccggaa catttgcctg caggtgggac atgcaaagac
    14281 agcgatcggt ttcccgaggg aaatccaatc ctcccgaagc ttggcaacgg ccgcctcatg
    14341 aagctgttca tctcccgccc attcggcagg agcgccgcag cagccgagta tcagggctgt
    14401 atccggggtt ttttccctca ggaatcggta gctttcaata acataccaag ggtccgatgc
    14461 tcccagctga catcctggga agaatacata cctgcttttg tcaaacccct tcggaagctt
    14521 acacattcct gctgcctcgc tgttggtgaa ttccatatcc cgcagccaga aatcatgaaa
    14581 tgcccacggc atggcacctt ttcctcgcat tgcccggtgg ctttgcagca gaaaatcccc
    14641 tgtgtcaatt tcctgcgggc agattccttt gcaaagtccg cattggttgc aggtagaaat
    14701 aaggcgggtt gcgacagttc cgtttccgtc cagtgttccg gggttaactg taatttctac
    14761 ttcttctcct attcttttgg gatattttcc ataatagctc atcaagtcac aatgacgtac
    14821 acaagcgtca caggagcacc ttagacagcg tttggcctca tacagggcat tttcctgggt
    14881 aaatacttct ccttcatcgg gaagcacagg cttcatatag ctgataagat cagtatttat
    14941 caatatttcc gttttgtcct ttgctttagg ctgattcatg ttaccggtct ttatataccg
    15001 ttcaatggcg tttatcgcct gaagcccatc ggcaagggcc tctattatat ttcttccgca
    15061 taattctccg cccataaaaa caccgggttt gtcactggca aaggcacctt ccggatcagg
    15121 cttcagtcca aaatccgtcc cccctttacc ggtggcaaca tatatggcat caaagtccaa
    15181 ctcatccaga cttgttattt ccgtattcaa gttaagggta tacttttcat gcataaactg
    15241 ccgctcaatg tcggccaaaa aaacttcagg gggcagaaca tcccataaat gcccgcctat
    15301 cctgtctgtt ctttcaaata ccgttacatt gtattttttt gacgccaatc ggagcgcaca
    15361 tccaagtccg ctgatgccgg cgcctattat tgctattttc ttattctttg gaggcaggtt
    15421 atagctattg gggtttaaat ccctcgcata ctttatggca gccttttcaa gatgcttcat
    15481 ggcgattgcc tcgtccttca agcgtctggg acatacgttt ttgcatggct catggcataa
    15541 ttcggacaca atgcccggga atccaactgt attcaaatag gttctgtagg ctgcattaaa
    15601 gccgccgcgc tgcattttgg gcataaagtc atgaatatcc agacgaaatg ggcaggcagc
    15661 agtgcaaaag gcaggttcgt tctgcaggca ggtttttatt ttttctttaa acttttccat
    15721 ttcaagtacc aatatgcacc tttctcagct aggataaatc attatggtat tgttgcgccc
    15781 gaattatgca attctgcgta atccgggcgc aaatcaatat ttcagttcag ctgcaatctt
    15841 tgattagaag aatctctctg cggtggcgtc ccctaacggg ataaagaaat catcgccgcc
    15901 gtacttaacg ggatttgcct ttatgttttc aagttcatcg tacaagtcgg agccaaggaa
    15961 gtatttcttt ggcggttcta tctttccgcc ggctgcgagt atatccagcc ccgctttcac
    16021 cttttcaggc gtagcaggca tctcatagat gcgcacacca caggcattat cgatagcgtt
    16081 cagaaccgct acatgcccgg atgactggaa agcttcggaa gctcccgagg aaccgaaggg
    16141 gccgacctca tccggccttt catagtgaat aacattgatt tcatccggaa tgtcctttat
    16201 atacggcaca ccggcgccta acatatttga atgcttttct acatcatcat aattttcact
    16261 gagggcaaag cctatgcagt gagatatgcc gccgtatgcc tggccgttta cggcatctat
    16321 gtttcctatt ttacctacat catccacgca ggtgaagcga aggactctta ccttgcctgt
    16381 agcagtgtca acttccacct ctgccaggtt caggcaataa gtataggcag gagtgggatc
    16441 tccgataccg gtattgggat ccaggcggca aatccccgga atggttgtat ttgaatatgt
    16501 agcttgatac tttgtcggaa ttccttcggc tgccatttca tcatatgtcc tgtaggtgcc
    16561 gtcaggctta cgcatagcgt tcataagctt ttctgcggca agtatggttg ctttaccgct
    16621 catgaagtgt gtacggcttg cacctgacat accgctgtcc gggcaaagct tggtatcatt
    16681 ctgaacaagc ctgatctgat ccggttttac ccccagaggc ttaagcgcct ccaatgttaa
    16741 cattaaggaa ccgatatcac cgccctggcc caattcctga taggtatcat atttagtgaa
    16801 ggtattgtca ggattaagct ctaatgcaac ggaagaactg tcggtaccgc cttcagtgac
    16861 attgaagcct ccccatgaaa gacctacgcc gcggcgcttc tgtggtgtat cagctgcttt
    16921 cgcttccgca acagcctttt cataataagg ccgcataatc ttcattattt cctccatagg
    16981 atactggcgg aacgggtagc tgtttatatt ggtgtcccct tcccttgcaa tatttatcca
    17041 gcggaactcg aaaggatcaa ttccggcttt ctttgcaagc atatccatca tagcctcgga
    17101 taaagtatac gcctgaggag aaccgtaagc gcggtaagct gtaccgaagt tatggttagt
    17161 gttggctata cgggtaaggc cggctgcatt aggtacctta taggggaaga aagcaaagcg
    17221 gacttgtttt gtcatcagat cgtctccgcc ccacgagtag gcaccatgat ccatcccaaa
    17281 gtcaaattca accgccgtaa ttttcccatt ttcatcacaa gccgcacgcc cgttggagtg
    17341 gcaggggcag cgtttccccg aaaaatgctg gtgctcttca tagctcatgg ataaagctac
    17401 cggcattttt gttactacgg ctgcagcggc agccaggcaa atatctcctg cgttggtaga
    17461 ccaaccgaag ctggctcctg tggggttcat gacaatgcgc aatttatcct ttggaatgcc
    17521 gactgaatcg cccatacgtc cctttgatga ataaacccct tgagatttgc actgaattgt
    17581 cagattgtcc tcttcatcaa agtaagcctg gactgtagta ccttcaatgg tcaaatgcgg
    17641 ttcccgggta gaataaaagc taccggacac actgtaagca gaattgtcta ttacttcctg
    17701 caccttggca gcatcttcca gacctacgcc cttcaaaacc ggctgcatag agaaaatatt
    17761 gggtgtatcc tcatgaacac gcattgcatc cggcataaca gcatccagat agctaaggta
    17821 ttcaggcagc ttttcaattt caactttgac ctttgccgct gcagcacggg catgctcctt
    17881 tgtatcagca acagcaaggg ctacaacatc gccataacgg aatatcttat cctcacacaa
    17941 caccttacgg ctgggaacca taactgtagt acgttcatgg aactgagcct cggccatcac
    18001 atttgtgcct ccggcctcct tgagctcctt gtgcgtaata atctttacaa ctcccggcat
    18061 cttctctgct tcagaggtgt caatattcaa aatctttgca tggtgggcaa ccctgggctg
    18121 cgttgctgca acatggagag tctctgccgg catcttaagc tcctggtcat ttccataatc
    18181 ggcaagtcca caaaccttgg caagtgctgt aggacgaaca accggcttac cataatactc
    18241 tttgtcctcc ggaagtttaa aggaaatgtc ttctattgtt gcctcaccgc ggacaacctt
    18301 tgctgctgcc ataacagcat caacaagctg tttatagccc gtacaacgac aaacatttcg
    18361 gtgtttctga aaccaatccc tgacctcttc acgggttggg ttattatttt cctgcaaaag
    18421 tgcataagcg gatacgataa acccgggtgt acagaaaccg cattgcacag caccatagtt
    18481 catccatgcg acttgcagcg gatgcaagtt attcggggta ccaatacctt caatagtaat
    18541 taccttgctg tactcttcaa cggtactgat ttttttcgta caagagcgaa tcactttgcc
    18601 gttcagaata actgaacaag caccgcatac ccctgtacca cagccaacct taacacccgt
    18661 caaccccata cggcgcagaa cagaagccag cgtgtccttt tccgggttgc aggcaaacat
    18721 tcgatttgcg ccattgacgt taagccacat ttttctaagc ttcattcaca tatacctcct
    18781 taatttttat gagtctgcca atattaaaga taattgtaat aattattatc tgtctgatgg
    18841 aataatattg attgtttgat tatataaaaa acggtgcgcc caaaaagatc gcaccgcata
    18901 tataatcaaa caaaaataaa aatgattact actgcgatta cgactcctct acaacaggtg
    18961 gcataagtgt ttccctatat accctaatgt tcttattccc atagcataag ctttacttaa
    19021 tagaatcgga gtcaattacg taattctgtt cataataatg atatactagg catgttattt
    19081 ttgtcaatat tggcgtataa tattattcac aagagaccct ccttggtgtt tattttgttt
    19141 tgcagctaat attttaccaa aaagagtggt ctcttgctat tttttgacct acaacaattt
    19201 tacactaagg tattacatgc attccataga ctctgaaatg atattccgaa ttgcttcatc
    19261 tcttacgaca gcccttgaca tttcaaagcg taaaaattat attctaatat aaggcacata
    19321 gtgaattcat cttatattac cggaggtgtt tcagagctat ggaaaacatc atgacattgg
    19381 aaaaagtcaa tgagcttcgg tcccagggct ttaactgttc ccagattgtg ttcctgtacg
    19441 gatctgaaag gttagggctg gaaccggaaa aagcattaaa aatcgcagga gctttcggcg
    19501 gtggaatgta taatggtgaa acctgcggct gtgtaacagg cgcactaatg gttttgggat
    19561 taaaatatgg ccaatctgtg ccctatgatt tagcggcaaa gaatcttctc attcaaaaaa
    19621 agacagaatt tgaagaaaaa tttaaagaag ctcatgaaac cctcatctgc aaagaaatac
    19681 ttggttatga catctccaat ccttcagaaa aatcaataat tgtggaaaaa ggattgctgg
    19741 acgaaatctg cccttatctc atatgggacg cttgccagat acttgatgaa atgctgtaaa
    19801 cactaatgct atgtgtttca aatcattgat ttccctccct tggaagcaca aatcaattcc
    19861 ggttatctgc ggtacaaaat aaaagatagg tacactattg agtacctatc tgcactaaag
    19921 cttcccatta cctatcagtt acgttagcgc ttaaatttga atggatcttt ttctccagat
    19981 accccctatt cttaagcatt tcccctataa aggttgagcc caatatcaaa atcccgccaa
    20041 tgatctgagt gaatattaac gtctctccca taaaaatcac actgaataaa ataccaaata
    20101 aaggttctaa ataactgtat gatattattt caacagattt catatttgtg tagagggaaa
    20161 agaacatggt ataagccaca cctgtatgca atacccccaa cagaatggta tatatagctg
    20221 ctttggcatc caggtgtaca gccgttaaaa gatttccttc aatcaataca aatggcagta
    20281 aaacaagcat cgctgtaaat atttgcacaa aagtggcagt ttggttatcc actctgactt
    20341 taatgctgcg gttaataagc acaatgcttg cataaatcat ccctgataca gcactaagca
    20401 gcatccccat gtatccgtac ccctccagta aattatggcc aacaatcaga aagaggccaa
    20461 gaaacgaggt taaaatcact gcaacttgaa tttttgaaat agtctctttc aatgccagcg
    20521 gtgcaaatat catgacatat accggggaca tattgtagat aataacagca gatgcgatac
    20581 tagtattttt gaacccatag aacaaagtta accagccaaa acccagtaat atccctgaaa
    20641 taaggtatgg ctttaaaagc gtccacttca acttatctgc ttttttcatt ttcattacaa
    20701 caaaaagaac cggaagcgca attgatgccc gaagaaatgc caagcttaaa gcggacaatg
    20761 gaatggattt tgtaaataca cccaggctgc cccaaataat cataacaagt actaatttaa
    20821 ttctattcat aatcatactc cttatctttg ataaatccag attagcagaa ggataaggag
    20881 ctgtattgta taaaatttac actatcccat taattgtttt atatatgccc ctggcgtaac
    20941 accatacatt tttttaaatt cccgaattaa atgggcctgg tcataaaatt ttaattccac
    21001 acaaatttca aacacatcgg cgcctttact taataacctt tttgcttcag ctacccgata
    21061 ttgtaaatgg tatgcagcag gtgtggttaa atataacttc ttaaaattcc tgattattga
    21121 aaatttattt agcccggata tttgctgaag cttatctaat gttatatcat cactgacatg
    21181 atttagtata taatcatgga tctttttgat attatcaatt gtatttgcag cagcagtttc
    21241 cggaatatca tcttctccaa actctatcaa caattcaata agtatatttt ccaatgtgtt
    21301 tggaatcttt tccgttagta gttgctcaat aaaacctact aacctctgaa gctgattacc
    21361 tgttaacttt tttgcttgat taaaagttac taaatcgcca tagtaaccgg gatcaacaaa
    21421 taacataaca tatgcccagt gattgatatc attcggcgca catctgtgag tcattaacgg
    21481 cggtataatt acaccatcac ccggtccgta atggatagta ctgccgttca atgttaagtc
    21541 agtggttcct tctacgatat atcccaatga gagttcgtta tggacatgag gcttataagc
    21601 atgggggtcg ttcctgcaaa gcttgatttc cacgccttca ataatactcc tgctaaattc
    21661 catgcggctt ttatctgaca tattcaaata caaatacctc ctcccattct attaactact
    21721 gcactatatc aattcaggtt tctgcactct taattcttta tataattata acatcatttc
    21781 cataatttgt tcattgctat tgacatattt tgcacgaata tattgcagca ataagcagtg
    21841 gtttatacaa taaaagatag gcatttttta ataatgccta tctcatataa atatattagt
    21901 ctgtacagtt tttcagataa tatacaggtt aattacactt cgaataacca cagttcctgc
    21961 atatcacaca gccgccttcg tgctgtatcg tgctgccgca ttccgggcaa ttcttcatat
    22021 tcccattgaa gctgccataa gcattgtatg atatactgcc ctcctcaaca gtcactgcat
    22081 ggcagggttc cgcacaaagg gagcaattat taatgcagtt tcccctttcc tcaatttcca
    22141 ttgccagttc atccattgtc ttgatatcct tattatccac cttggtattc atatatcttt
    22201 cgagggccct tcctattgca tcagggcagg aaagtacctt aagccctttc tgtctaaggg
    22261 tcgaatgaca tctgatgcct ctgagctgct ctattatctc ccctatatcc actccggccc
    22321 tcaaggcaat tgaaataaga cggcttgttg cttcagactg gcttggacag cctcctgctc
    22381 ttccaaggtt tgtgaatacc tcacaaatgc cgttttcatc tgagtttacc gttatatata
    22441 aattgccgca gcctatttta actttctcgg ttactcccgt tgtcacttcc ggtctggttc
    22501 ttggcacaag aacctcattg gccggagccg gtttttcctt attatccttg tttgccgagc
    22561 cgatgttcag cacctgggat tcgcggctgc cgtccctgta tatcgtcacg cccttgcaac
    22621 ccatcctgta agccagcata tatacggcac ttacatcatc cctggcagcg gaatttgaaa
    22681 agttgactgt cttggatacg gcattgtcgg tatacttctg gaaggctgac tgtatcctta
    22741 tatgccactc cggctcaata tcgtgggcag tgacaaatac ttttcttata tccttcggta
    22801 tgctcttgat atcctgaatg gtgccatgag ctgcaatttt cttcatgagt tcagtgctgt
    22861 aaaagcctct ttccctggca accctttcaa acaacgggtt tacttcggga agctcatcat
    22921 tatccattac gtttcttaca aaggacagtg caaacagcgg ctctataccg cttgaaactc
    22981 cggcaattat ggatatggtt cctgtaggtg caattgttgt tgttgtagca ttccttatcc
    23041 tgatgccttt ttccttaaac acgctgtcct catagtattt gaaagtgcct ctcttttctg
    23101 caagcttcat tgaagcagcc tttgaccttt catttatgaa cttcattatg ctttctgccg
    23161 tattgaccgc ttcttccgag ttatatggta tacccagctc taccagcata tcggcaaagc
    23221 ccatgactcc aaggccgatt tttctcgtcc cctttgtcat ctcatcaacc attggcagag
    23281 gatatttgtt gacatctata acattatcaa ggaagtgtac cgattcatct actatactgc
    23341 ctaacttttc atagtctatt accttcccga acttgttctt cttcttaatg cagctcatga
    23401 ggtttataga acccaaattg caggattcat acggaagcag aggctgttca ccgcagttat
    23461 gtatatatat gccgttggca tcaaaagcgt ttatccccgg gacctgaaca tcaaacactt
    23521 cagcttctcc cgattcgata atctccgtaa cggaagcaac aaaccgctct ctattcaaag
    23581 ctcgtttgta attgcccaat ccggatttta agcgattctg tttcttactg ctgctgaagc
    23641 ctattaattc atggaaaaca tataagttgt ccttagaaat aaccagttca tggcctgcct
    23701 ttacattata atccttcaaa ccgcctttgc cgtcaggcat tgatttaatg cccggctcct
    23761 tacgattact gtatatggca gatatgattc ccattctgcc cagcattctc tgcacagcct
    23821 taagagtatc caggtcattc tgccaaagtc ttactgacag acctttttcc tgtgtacccg
    23881 caacagttcc gtcggtatcg aacatacccc ttaaaaaccc ccgataaaac tcatagcttc
    23941 ccttttcaac ctctgaagca atctttttgt ctccgggcat gactccatat tccaaagcaa
    24001 ggtcacgaag tgcagaaagc ctcatccggt tttcttccct atcgctgatt actgtttgaa
    24061 atcctctgaa gtcgcttcta tgaggcaatg cacaggcagc ttcacctgcc gctttcatta
    24121 aggatactgc ctcttcatcg ctgccccata cggatattat tcctccctct tttttaagtg
    24181 tcccatcccc tataagcaca cctaacagat atccttcatc aaagcttcct tttccttccc
    24241 atcttatact cctattgttg gacataatga ttctgtctcc gggcctgaga tcccctgccg
    24301 ccttccattc ctcatcaatc ttaaaccgcg tcatattcgc tgccgttctt atcaaatggt
    24361 cctttgtcag cttcagttca taaccccggt cggtacaaac cctgattata ctttttttgc
    24421 cggtgctgaa gaaccccttt tcatctgaca tggaatatga accgtttagt gcaagctgta
    24481 cctgcctgcc taaaatatcc ttaacctgaa cagggccttt tcgggtcaga acccatgagt
    24541 cccctgttat gcacggattg gtgctctcga tttctcccag cctgggtacc gcattatccc
    24601 tgtttatcct gtcaaggaat atgattcccg gctctccgtt gttccaagcc atatctacta
    24661 taaggtcata aacctctttt gcattaagtt tcccggcagg tttcccggtc gcagggtcaa
    24721 taagctcata atcttcgccc ttttctgcag cttccatgaa cttttcagta atggcaacac
    24781 ttatattgaa attggttata tcgctgttat cctttttgca ctgtataaac tcaattatat
    24841 ccggatgatc caccctaagg atacccatat tagcgcctct tctggttcct ccctgcttca
    24901 cagcttcggt ggcggcatta aacaccttca taaagcttac gggccctgat gctacgccgc
    24961 cggtagtcgc cacagccgat cccttttttc ttaagcggct gaagctgaag cctgtgccac
    25021 cgccgctttt atgtatcatt gcagcatttt tgatagcatc aaaaataccc tccatagaat
    25081 cctcaacagg aagcacaaag catgctgata actgtccaag ctcccttccc gcattcatca
    25141 aagtgggaga atttggaata aactcaaggc tgctcataag cttataaaat ttttcttctg
    25201 tttctgcaac atctgccgga ctgccgtaaa aactatcagc agccgctatt gtgcttgcaa
    25261 ctctcctgaa catatgctct ggggtctcaa ccggctttcc gttctcatcc tttataagat
    25321 aacgtttttc caatacttta acggcattgt ctgttaacac ggtaaatcac cacctccggt
    25381 ctgtattttg tatcttgctg cgtatctgaa cactatatat tgtatcatat ggcaaaatat
    25441 aatactattt atatttgcta aattatttgt caaaatttcg acaaataacg gggacatggg
    25501 gacgttcggg gacatgggga cgtttctcct gtcccgctga aagcgggaca ggagaaacgt
    25561 ccccatgtcc ccgaacgtcc ccatgtcccc aaaatatctt cttgtacctg ttcctgctct
    25621 attccttgag caatgcgcct gaagtgaata cattgcctgc gttccacagc agccattctt
    25681 cctggcctgc atcataagtc gcatctatct gagcccttac ttcggcaggg ccgtatcttt
    25741 tatacctatc aagatatgaa gctgtaaaat cctgaagcca cggccttaat accgccttcc
    25801 tccttacatc cttcatggca tttattcttt cctgtgcaac ggacatactt cggttaacta
    25861 ttttataggg ttcaaagtcc ggctccgcca ccccatagct cccttttccg taatgggacg
    25921 ggtaaaccat cggacatatt acgtcaacac ttttggctat tgactccagg tgctggccta
    25981 ttcgcatatc gtccgtggca gttgtaacca gcccgaaaac atcagctgat accacaactc
    26041 ccattttgct cagcctttcc cttccgtagg acagaaattc cgcaattgcc tctgctttgg
    26101 actttcctgc tgcactggtg ccgtagtcga taatctttgt gttgccgtcg gtaggaaatc
    26161 ttacataatc aaattgtatc tcattaaaac cttttgctgc agcttcttct gcaatgtcaa
    26221 ccaaataatc ccatgattct ctgttgtatg gattcagcca ggacattccg tgccggtccc
    26281 tccatatccc gccgctggta ttctttaccg caagattggc atatttgtct ccggctatcc
    26341 tatccttgaa agtaactatt ctggctatgg aatatatatt attgccacgc agtatcttca
    26401 ttactgcatc tatatccttt atgcgtactt ttttgtttgc tcccgtaaaa tccacatcag
    26461 gaaggtcgct ggagtaagtc atcaatccgt tgtcatcctt cacgtcaatt accacggcat
    26521 ttaattctgt agtctttacc aggtcaatca gcttgttgaa cctttcggtc atggcaacag
    26581 tatatccggt catatagatc ccttttactt tgagcctttt cttcatcagc tcccagtcgg
    26641 gaccctcctg ctgcaagtag caatcttttt cataagataa atcaatttcg ttttttccat
    26701 aaatatatct ctctgcattt gctgcgttca tttgaaacat aattcccaaa agtatcactg
    26761 ctgcttgtat tgtccatttt ctcatcatat aactcctcga taaatgaagt aatttctgat
    26821 aatagtatat cataaaagca atacggatat tatccttatc gctgtttatg ccaaaaaatg
    26881 cgccttatgg cgcattttgt caaataatat cttataatgc tgtctccgct atataatgac
    26941 agccggtatg aaaagcggcg gcgttatcat gcctaatctt cgaatactgc gccggtattg
    27001 gcagaagtca ccatcctgcg gtagcgcttg agatatccgt tcggtacagg tttttcaaaa
    27061 ggtacaaatt ttgtctttct ctctgctatg gttttttcat caaccaaaag ttccagttta
    27121 ttattcggta tgtctatact gataatgtcc ccgtcttcga caataccaat cagtccgcct
    27181 gcagcagctt caggagatat atgcccgatg gatgcgcctc tggatgcacc ggagaatctg
    27241 ccgtcggtaa tcagcgctac actgctgtcc agtcccattc ctgcaataga tgaagtcggt
    27301 gaaagcattt ctctcatacc gggaccacct ttaggccctt cataacggac gacaaccaca
    27361 tctcccggta caactttgcc gcccataatt gcagccatgc aatcctcttc gctgttgtat
    27421 acctttgccg ggcccctgtg aaccatcatt tcaggaagca ctgccccctt cttgactacg
    27481 gctccgtcag gtgccaaatt gccccaaagt attgcaagtc ctccatcctg ggagtatgct
    27541 gtttcttttg tccttattat ctccctgtcc tttacggtta catctttaag gttttcagct
    27601 gctgtgcaac ctgttacggt tatgcagtcc gttttcaaca accccattcc ttccagctct
    27661 ttcaaaactg cctgtacacc gcctgcctgc cacaaatccg acattgcatt gggtccggca
    27721 ggggcaagct taacaaggtg cggtgtcgct ttgcttatat catttatatc tttcaaatca
    27781 attttcaatc ctgcttcgtg tgcaattgca ggaagatgca gcacggtatt ggatgagcat
    27841 cccagtgcca tatctgctgt aagtgcattg tgaagcgaat cagctgtaac tatatctcgg
    27901 ggccttatat tcttctccag catttccata accttcatgc ctgccagttt ggcaagacgg
    27961 acacgagccg cattaacagc cggtattgtt ccgttcccgg gtaagccaag cccgattgct
    28021 tctgtcatac agttcattga attcgcagtg aacatgcctg cacaagagcc gcagcccgga
    28081 caagaataat tttcataatc cagaatttcc tgttcgttca ttttgttagc ccctgctgcg
    28141 cctacagctt caaatgtgct tgaaagtgtc agtttctttc cgtccaaaaa agccggcatc
    28201 attggtccgc cgcttacaaa tacgctgggg atattcagcc ttaaagccgc cataatcatt
    28261 cccggaacta ttttgtcgca gttgggaatg aacaccaatg catcaaaggg atgcgccatt
    28321 gccatgattt cgacagaatc ggcaatatgt tcacggctta taagagaata cttcattcct
    28381 tcatgtccca ttgccaatcc atcacatacg ccaattgcag gaaattggaa gggagtccct
    28441 cccgccatcc gcactccggc ttttactgct tccataatat cgtccagatg cagatgaccg
    28501 ggaattacat cattttgtga attaacgata ccaatcatag gccttctgat ctcttcgtcg
    28561 gtgagtccca gtccctttaa cagggccctg tgcggaatcc gctctatgcc cgccttcatt
    28621 tgatcacttc tcattcagtt ttccctccgt ttcagattca ttaatataat aggattaaag
    28681 ctgaagcaag cacctcattg gaaattaaat gttgtatgac gtctcgaaaa cttttctgat
    28741 gaataactga taatcaaaca caatatccat aagagttgta tgatgtctta atccaatact
    28801 atgccttttt tgtcataatg tcaacataat tataaaattt tatatagaga gatactacct
    28861 ctttcttaaa taaaaaacta taatgggctt tggaaaaaat gtggtataat gtcttatata
    28921 agtttaaagg agaaataata tggctaatgc ttttatacct atatcac
//
LOCUS       DAOQUR010000030        27361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2063_length_27361_cov_11.955578, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000030 DAOQUR010000000
VERSION     DAOQUR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 27361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27361
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_27361_cov_11.955578"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1109)
                     /gene="dusB"
                     /locus_tag="PKI50_07650"
     CDS             complement(129..1109)
                     /gene="dusB"
                     /locus_tag="PKI50_07650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNT03612.1"
                     /translation="MQIGNVKLKNNVFLAPMAGVTDMAFRILCKRQGCGLTYTEMVSA
                     KGLHYKSDNTAVLLEIAEEEKPAAAQVFGSEPDIVAEAARQAEAGGAAIIDINMGCPT
                     PKIVRNGDGSALMKSPVLVGEIVRSTVAAVKVPVTIKIRKGWDEESVNAVEVASIAAM
                     EGAAAITVHGRTREQFYSGTADWNIIKRVKDAVDIPIIGNGDVITPQDAGRMLEETGC
                     DAVMIGRGAQGNPWIFERTAEYLKTGELLPEPDFEQRILTIMEHMDMVTELKGEGTGV
                     KEMRKHAAWYLKGMPGSAKVKAEIFKLTTCSQVKDILSQYLDYLQKHISS"
     gene            complement(1121..1891)
                     /locus_tag="PKI50_07655"
     CDS             complement(1121..1891)
                     /locus_tag="PKI50_07655"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNT03613.1"
                     /translation="MILVIDVGNTNIVLGIYDDRKLVSFWRVKTDAEKTSDEYGMIIN
                     QLFELQGHKFSDIEAVVICSVVPPIMYTLEHMTRKYFNREPLVVGPGVKTGMNVKYDN
                     PKEVGTDRIVNAVAAYELYGGPLIIVDFGTATTFCAVSKNGEYLGGAISPGIRISMDA
                     LFERTAKLPRVELAKPSTVICKNTINSIQSGVIYGYVGQVDYIVRRMKKELGDMNTKV
                     IATGGLSKLIASESETIDIVNGLITLEGLRIIYDRNRE"
     gene            complement(2023..3012)
                     /locus_tag="PKI50_07660"
     CDS             complement(2023..3012)
                     /locus_tag="PKI50_07660"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNT03614.1"
                     /translation="MKNRILEELRANSGQPVSGEEISRRLGVSRTAVWKHISKLRSEG
                     YSIESHTNSGYKLVGSPDILSSSELEPYLHTEFMGRNIIYLDSIDSTNTYAKKKAEGL
                     FREGTVVIADEQTGGRGRLGRHWVSTKGKGIWMTLMLKPDILPSDAPKLSIVAALAVT
                     NAIRSCCRLDAEIKWPNDIVAGGKKLCGILTEMSAEEDEIKYVIIGIGINANMDNDDF
                     GQEVSSIATSVRIESGMPVSRKALAASVLFEFEQIYRVFTRDGSIKAFLEDYKSKSAV
                     LGKEVRLISKKEEIKGLAVDISKEGHLVVRLADGSYREIMSGEVSIRGMHGYI"
     gene            complement(3142..4314)
                     /locus_tag="PKI50_07665"
     CDS             complement(3142..4314)
                     /locus_tag="PKI50_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNT03615.1"
                     /translation="MGFAAITWQQAVMLCLSCVLMYLALKKGFEPLLLVPIAFGMLLA
                     NLPVTGLMAGPVYEIATDPVTGEHVHQMKQAGGVLYYMYQGVKLGIYPPLIFFGVGAM
                     TDFGPLIANPRSILLGAAAQLGIFTTFIGAILLNLVPGLSFTLQEAGSIGIIGGADGP
                     TAIYLTAKLAPHLLGPIAVAAYSYMALVPLIQPPIMKALTTPEERAIVMEQLRPVSKI
                     EKIVFPILCTLVVSIILPSAAALIGLLMFGNLLREAVDLQSVLNRLSKTAQNELMNIV
                     VIFLGVAVGATANGEIFLRAQTLAIIALGLAAFSMGTAGGVIFGKIMCKMSGGKVNPL
                     IGSAGVSAVPMAARVSQKVGQEANPSNYLLMHAMGPNVAGVIGSAVAAGVLLAMLG"
     gene            complement(4419..4820)
                     /locus_tag="PKI50_07670"
     CDS             complement(4419..4820)
                     /locus_tag="PKI50_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNT03616.1"
                     /translation="MRKFMINVNGNSYEVEVEEIKEGAAQATAPVTAPRPAAPKPAAA
                     PAAAPKPAPAAAPAAAPAGANTVTAPMPGTILSINVKAGDAVKKGQVLCILEAMKMEN
                     EIMAGADGTVASVAVSQGDSVSTGQVLITFA"
     gene            complement(4845..5216)
                     /locus_tag="PKI50_07675"
     CDS             complement(4845..5216)
                     /locus_tag="PKI50_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNT03617.1"
                     /translation="MTNFSDTFTYALIVTLFGMGIVFLVLILLQYMLKAMEIIFHREK
                     KAVQEPAQVSAVKPAEPDEPQVNEAEEASDDQLAAVITAAVISCLGGNSNIAVRNIRR
                     VEDLTPAWGKASRTEQMAVRF"
     gene            complement(5238..6785)
                     /locus_tag="PKI50_07680"
     CDS             complement(5238..6785)
                     /locus_tag="PKI50_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNT03618.1"
                     /translation="MSIDKINDLHSRLDKITAAGGADRIEKQHKAGKLTARERINLLM
                     DPDSFVELDAFVKHRCSEFGMEAVDAPAEGVVTGYGTVDGRLVYAYAQDFTVIGGSLG
                     EMHAKKICKVLDLALRMGAPVVGMNDSGGARIQEGVDALSGYGNIFYRNTIASGVVPQ
                     ISVIMGPCAGGAVYSPALTDFTFMVDKTSQMFITGPQVIKAVTGEEVSPEELGGAMTH
                     NRTSGVAHFISSDDKACIQDIKRLLSFLPSNNLETAPIFETDDDINRIEEQLNEIVPD
                     NPNKPYDMRDIIRAIVDNGDFMEVQEYYAQNIITAYARINGASIGIIANQPKVLAGCL
                     DINASDKAGRFIRTCDAFNIPILNLVDVPGFLPGTNQEYGGIIRHGAKMLYAYSEATV
                     PKVTLIVRKAYGGAYLAMCSKDLGADMVFAWPSAEIAVMGPDGAANIIFKKDIEGAED
                     PVQTRAEKIQEYKDKFATPYIAAKRGYVDSVIEPSESRPRLANAFEMLASKRESRPAK
                     KHGNLPV"
     gene            complement(6813..7232)
                     /gene="mce"
                     /locus_tag="PKI50_07685"
     CDS             complement(6813..7232)
                     /gene="mce"
                     /locus_tag="PKI50_07685"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNT03619.1"
                     /translation="MKGGIQLVSKIDHIGIAVSNLDEAVKLYRDVLGLELHGTEVVPE
                     QKVKVAFLPVGDTEVELLESTSAEGPIAKFIEAKGQGIQHIAFRVDDIEAALEEMKAK
                     GMRLIDEKPRYGAGGAKIAFLHPKSTGGVLIELCERK"
     gene            complement(7277..8227)
                     /gene="meaB"
                     /locus_tag="PKI50_07690"
     CDS             complement(7277..8227)
                     /gene="meaB"
                     /locus_tag="PKI50_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNT03620.1"
                     /translation="MDIKAGLLNNDKRAAARLISMMENGEPEAVQIIKECYNMTGHAR
                     IIGITGPPGVGKSTVTDKLAKQLRKEGKQVGIVAVDPTSPFTGGAILGDRVRMADLNN
                     DPGVFIRSMGTRGALGGLSKAVHGAVKVLDIFGMDYIFIETVGVGQSEIDIVKLADTV
                     IMVMVPGLGDDIQAIKAGIMEIGDIFAVNKSDRDDAERTAAEIEAMLDFNRNEEYRPP
                     VLNIIARDNKGIEKLLELIEKHWEYENKSGSYKQKKLNRLRTEIMDIFRDEVMKTLYS
                     ISENTNIINELAQKVMDKQMDPYTAADRLMDEFTNNQRNN"
     gene            complement(8240..8638)
                     /locus_tag="PKI50_07695"
     CDS             complement(8240..8638)
                     /locus_tag="PKI50_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNT03621.1"
                     /translation="MDRPIRVVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQTP
                     EQIAETAIQEDADCVAMSILSGAHNHLFPKVVNLLKERGADDILVVGGGVIPEDDIPG
                     LIEAGIAGIFTPGTPTSVTIDFIKNNIKRK"
     gene            complement(8818..10494)
                     /locus_tag="PKI50_07700"
     CDS             complement(8818..10494)
                     /locus_tag="PKI50_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNT03622.1"
                     /translation="MFNKEKLEQLKKAHEEWENTVTKAVAKRPERKKEFETSSGIAVK
                     RAYTPADIENLDYDRDLSNPGEYPYTRGVQPTMYRGKFWTMRQYAGFATAEESNKRYK
                     YLLEQGQTGLSVAFDLPTQIGYDSDHPLSEGEVGKVGVAIDSLKDMEILFDGIPLDKV
                     STSMTTNAPASVLLAMYIAVAEKQGVSSDQLSGTIQNDILKEYIARGTYIFPVEPSMR
                     LITNIFEYCSKHVPKWNTISISGYHIREAGSTAAQEVAFTLADGIAYVDAAVKAGLDV
                     DEFGPRLSFFFNSHNEILEEVAKFRAARRLWAKIMKERFKAGDPKSMMLRFHTQTAGC
                     TLTAQQPDNNVVRVAMQTLAAVLGGTQSLHTNSRDEALALPTEDSVRIALRTQQIVAY
                     ESGVTETADPLAGSYYIESLTDQIEQKAVEYLNKIDEIGGAAKAIEKGYIQKEIQDSA
                     YKYQKEIEAGERIVVGVNKFQIKESAPKGLLKVDPKVGELQQKKLAELRKIRDNTKVN
                     AALEKLGNAAKGEDNLMPFILDAVKAYATLGEICGVLRKVFGEYQQSIVL"
     gene            complement(10767..11171)
                     /locus_tag="PKI50_07705"
     CDS             complement(10767..11171)
                     /locus_tag="PKI50_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT03623.1"
                     /translation="MDNNLKIGITAQVGKTVSVDDTAVKFGSGGVRVFATPMMVALME
                     NAALNAVDPHLGEDSATVGLSLNVKHLAATPVGMKVTAAAELIGIEGKKLTFKVEAYD
                     EQEKIGEGIHERYIIKLSKFLERAAQKTSKVL"
     gene            complement(11248..13056)
                     /gene="ftsH"
                     /locus_tag="PKI50_07710"
     CDS             complement(11248..13056)
                     /gene="ftsH"
                     /locus_tag="PKI50_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNT03624.1"
                     /translation="MKKFLRGISFYILIIIIIYMFVLIIQNAEPAKDKLSYTQLVTEI
                     ENGRIKEVTVSDNNGMSVVTGKISNTRRFEAIVPSSEFNELVQDYIERDRLDVKYEIK
                     TAAAAWFSILPSIVLIVIFIVFFFVLTQQSQGGGSRVMSFGKSRAKLHTEDKKKITFA
                     DVAGADEEKEELGEIVDFLKQPRRFIELGARIPKGILLVGPPGTGKTYLARAVAGEAG
                     VPFFTISGSDFVEMFVGVGASRVRDLFEQAKKNSPCIIFIDEIDAVGRHRGAGLGGGH
                     DEREQTLNQLLVEMDGFSVNEGIIIIAATNRPDILDPALLRPGRFDRQITVGIPDVKA
                     REEILKIHARNKPISLDVNMETLAKGTTGFTPADLENVMNEAALLAARKGKKKIEVID
                     LDEATIRVIAGPEKKSKVVSEHDKKLTAYHEAGHAVVQKLLPNADPVHQISIIPRGRA
                     GGYTISLPQQDKFYMSKSELEEQIVSLLGGRVAEKLVLDDVSTGAKNDIERATGIARK
                     MITEYGMSDSLGPMTFGSEHDEVFIGKDLTRTRNYSEEVAAIIDKEIRKVIDAAYHKT
                     ETLLKENISKLHGVAQALLEREKLDQAEFEEVFASA"
     gene            complement(13290..13832)
                     /gene="hpt"
                     /locus_tag="PKI50_07715"
     CDS             complement(13290..13832)
                     /gene="hpt"
                     /locus_tag="PKI50_07715"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNT03625.1"
                     /translation="MLEDMKSILISEEEIAKRVKELGKQLAEDYKGKELLVVGILKGC
                     MLFLSDLVRTIDLPLTLDFMVVSSYGATTKSSGVVRIVKDLEREIEGKDVLIVEDIVD
                     TGLTLSYLVENFKARNPKSVKVCSLLDKPDRRKAQVEIQYIGFKIPDEFVVGYGLDYG
                     ENYRNLPFVCVLKPEVYEKK"
     gene            complement(13837..15225)
                     /gene="tilS"
                     /locus_tag="PKI50_07720"
     CDS             complement(13837..15225)
                     /gene="tilS"
                     /locus_tag="PKI50_07720"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNT03626.1"
                     /translation="MTGFIENVRKTIAKYDMVKPGDKIVIGVSGGPDSLCLLHVLKGL
                     CREYGCSIFAAHLNHKFRGKAADSDAEFVEKICREWDIPVFIEVFDVPAYIKETGLSP
                     EEAGREIRYRLFRRVCEEVSGNKIAVAHNLNDHIETILMRFMRGSGIEGLKGIEAVRG
                     NIIRPLLETDRLSIEDYCAFYNLNPRIDKTNLESVYNRNKIRLELIPYMEKNFNPNIK
                     MALSRFSGLIKDENDFLETEAREKFKEVAEDSDDRVVIEVTKFIALHKALQRRIIRQC
                     AERLLNTLNGFEFKHFEKVLEIAHQSTGAAVMLPHKLKAFRSYDKLVLAKNIVKADKK
                     CYYKLKYDYDNSVDTDNGRITVQRIKAKEIAELKGQKDTIYIDPLKIKGELVLRCRQA
                     GDMFAPIGMKGTKKLKEYLIDEKVPREKRDDLELIADGHEIVWIVGGRLSEKYKISDD
                     TSDAVLIKYIRG"
     gene            complement(15471..16178)
                     /locus_tag="PKI50_07725"
     CDS             complement(15471..16178)
                     /locus_tag="PKI50_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT03627.1"
                     /translation="MDAWIDSGEIKQIIIVTDGKSGSGISLVEAAKEAYDKGIVVSAI
                     GIVDPEAGSESEAEEAKKIAEAGGGLWYYSKAEDLKYAVKEIAIDTSVKTIERIVDRQ
                     LKAIIGEGIPDLEPESRTKIMNFIRKYGGNINLKCIVVIDAGRIIKGGFTIAEKSAAG
                     LLESIRIRKGCSSIAVIDCSGETAGIHDTICEFAGDISLLNRNPELICCSGDGQAGAA
                     ILKACELMNQYYEVYNA"
     gene            complement(16271..18646)
                     /gene="spoIIE"
                     /locus_tag="PKI50_07730"
     CDS             complement(16271..18646)
                     /gene="spoIIE"
                     /locus_tag="PKI50_07730"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HNT03628.1"
                     /translation="MATVSNQSVTNKRGRIRHIIDFFMNTSFRMYMILSAFGLLLGRA
                     VILDFLNPFCTAFFAAAMVQKLNLFVMGTSILLGVLSVGDKGILLKYIIAAIVLIIPY
                     AATRKMKVNRKLVISAAAALINLAAGYFIFYVQNYYLYDLLMLIIESILIAILIYIYD
                     RSLPVIKNYKNRRLLSSEEVVAVAVMAAFCFVGTEIYIAGFSVKNIAVIFIIMIFAYL
                     GNVGTGAAVGIIMGIVQALSGSILPSAIGVYGLCAMMCGILKNLGRYGCPLGFIISNA
                     LMTFYINGSTEVLIRFYEILIASFLFVCIPQNYMKKLSGYKLSIAGEYLKEKSYSMRI
                     KENTIERLSEFAGVYKSLADTLKDTIQDRSYFSHTDAAQIIDQVVGKTCSDCGLAGSC
                     WKRNFYNSYQYLFNLLTIIENGGKLKKDGCYKGFKERCLKPEEIIENLKYYYDMYRNG
                     LGWKKKINESRLLVSDQLKEVSAVVSDLALRIDMDVDFDRDMEELIMVGLDNDAVRVK
                     DVIVARTGNTMDVDIRVDSCGGKRECIKNIIPIVNRVTGKKFNKREMTCDMIHGSICS
                     IKLKEAQRYQIATGIARMNKSGAISGDNYSFIELKDGKFMLALSDGMGTGPKAALESS
                     TTITLLEKFLYAGFDKDVALKAINSLMLLKSNDETYSTVDMTVINQYNGEVEFIKVGA
                     VSTFIKNEEGVQIIKTGTLPVGILSRIDVELVRKKLKDGDFVVMVTDGVLDCNREIVD
                     KEKWLAELIRDVDTRNPQRLAEEILQSCLQANGGEAPDDMTVMAAKVWEAM"
     gene            complement(18847..18923)
                     /locus_tag="PKI50_07735"
     tRNA            complement(18847..18923)
                     /locus_tag="PKI50_07735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18887..18889),aa:Met,seq:cat)
     gene            complement(18937..19012)
                     /locus_tag="PKI50_07740"
     tRNA            complement(18937..19012)
                     /locus_tag="PKI50_07740"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18977..18979),aa:Met,seq:cat)
     gene            complement(19020..19108)
                     /locus_tag="PKI50_07745"
     tRNA            complement(19020..19108)
                     /locus_tag="PKI50_07745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19072..19074),aa:Leu,seq:taa)
     gene            complement(19222..20145)
                     /locus_tag="PKI50_07750"
     CDS             complement(19222..20145)
                     /locus_tag="PKI50_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNT03629.1"
                     /translation="MKRIAVIDIGTNSMRLMLCKIEGNFIANKEKELMITRIGEDVSK
                     TGMITEKSFARNIEALKYFRNKADRYGAEEVYAIATSAVRDALNGGAFVADAKSQAGV
                     DVMIISGDEEAELGLRGVMSEIQNPQETVLVIDIGGGSTELVLGSKGKTDYKVSIPAG
                     AVRMTEHFVTGNPIRNEDVENMKNRLNEIFKEPLEHLGKKRIDKVVAIGGTATTIAAM
                     FHNMSIYDPETVHNTIVDSEYIESIFNKLKDMSVEERWNVRGLQKERADVMPSGIFIL
                     QHIIEGLKRDSLVVSENDNLEGIIEKYVLSK"
     gene            complement(20282..20707)
                     /locus_tag="PKI50_07755"
     CDS             complement(20282..20707)
                     /locus_tag="PKI50_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HNT03630.1"
                     /translation="MPIEEGKVVEGIVSSITSFGAFVQLPEGKMGLVHISEIADTYVK
                     DIKHFIKEKDKVMVKVLSVEKNGKINLSIKQAQEIKKTNRPIEIDWEQEARKNQGISF
                     EDRLSKFLKESDEKLQQLKKNTDSKRNGGYRKNDGLQKG"
     gene            complement(20847..21134)
                     /locus_tag="PKI50_07760"
     CDS             complement(20847..21134)
                     /locus_tag="PKI50_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNT03631.1"
                     /translation="MKKRKLRLKNIFMIAFIVYAAITIVNQQLTIYDLRKSEQDEISK
                     IEAIRNDNDRLMEMINNATSVEYIEKLAREQLGLVKPGEKVYIDQSSDEKE"
     gene            complement(21283..21822)
                     /locus_tag="PKI50_07765"
     CDS             complement(21283..21822)
                     /locus_tag="PKI50_07765"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HNT03632.1"
                     /translation="METVQFQSYVFLYSLYGGIIIGILYDIYRVIRGRKKTERLITSL
                     WDILFLFSVFAVVIWAIFSSSYGEIRSYVLIGFLVGFYLYEKLLGRIASGLFHLLYKS
                     ISSFFMKTGSILALPLKMACSFVKSCCCSLIRILGKKKIRLRKVCGLPKQVIYDSKKY
                     YRLIVKRKERKENSNKGFS"
     gene            complement(22084..22371)
                     /gene="yabP"
                     /locus_tag="PKI50_07770"
     CDS             complement(22084..22371)
                     /gene="yabP"
                     /locus_tag="PKI50_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HNT03633.1"
                     /translation="MEEKKLIKSKPHSITIEGRERLIINGVRDVASFNENEVSLETEA
                     GGLVIRGTDLHINRLNVDDGNLFIEGYIISCVYNDKIDIKKTGSFLSNIFK"
     gene            complement(22465..22704)
                     /locus_tag="PKI50_07775"
     CDS             complement(22465..22704)
                     /locus_tag="PKI50_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014095295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNT03634.1"
                     /translation="MRLDKYLKNSRLIKRRTVAKEACEGEKVYLNGKLAKPSAEVKVG
                     DIIEIVFGEKRIKAEITDISDNVSKASAREMYKLI"
     gene            complement(22790..23071)
                     /locus_tag="PKI50_07780"
     CDS             complement(22790..23071)
                     /locus_tag="PKI50_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT03635.1"
                     /translation="MNKSDLISKMAEKSAMTKKDSEKALKALVESVEEALAKGDKVQL
                     VGFGTFEVKARKARVGRNPRTREEIKIPASKAPIFKAGKDLKDLVNKKK"
     gene            complement(23156..24640)
                     /gene="mazG"
                     /locus_tag="PKI50_07785"
     CDS             complement(23156..24640)
                     /gene="mazG"
                     /locus_tag="PKI50_07785"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNT03636.1"
                     /translation="MKEITIVGLGPGDYKQLSLEALEIMKNADILVFRTEKHPVAERI
                     RESGISFTCCDDIYDSCESFDETYGQISRRILDLCREKGQVVYAVPGHPLVAEKSVEL
                     ILAGAGKDIKVSIVPALSFIDVILSSLKIDPIYGLKIIDGLSIKTQKPDKKCGNIITQ
                     VYDKLVASEVKLALMDYYTDDFEIYIIRAAGVEGQERIASIPLYELDRIEWMDYLTSI
                     YVPPALDSGARYKSFEDLVELMENLRGENGCPWDKEQTRESLKPYLLEETYEVLDAIE
                     RDDLELIVEELGDLLLQVVFHSQIAREDGEFDINEVITNIVNKLIIRHPHVFGDIKAT
                     SESGALKSWEASKRKYKGIDTYTKTLEDIPDILPALMRSYKVQQKAALAGFDWDEVEE
                     AFSKVYEEAEELKEVYKTGEKAIIEEEIGDLLFSVVNVSRFLKVQPELALRSTTEKFI
                     QRFKYIEETALQSGRKLDKMSLQEMDMLWNEAKQQVLAKKDEKR"
     gene            complement(24753..26354)
                     /locus_tag="PKI50_07790"
     CDS             complement(24753..26354)
                     /locus_tag="PKI50_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNT03637.1"
                     /translation="MGYRKQSFIKGAAILAAAGIISKALGAIYRIPLGRIIGSEGMAY
                     YQTAYDLYIMMLTISAYSIPIAISKLVSEKIETGRRDEAHKTFKVALSLIAFLGITLS
                     AILFFNAKGFATMVKNPGAYVAVLCVAPAIFTVALSGAFRGYFQGMQNMTPSGLSQVT
                     EQIARVVFGFILAITFLPMGYESAAGGAVFGTTIGGLVSFLTLIYIYRMKRGEIKAGI
                     RADLQSKKESALSLVKRIVVFSVPITIGGSIMPVMNVIDTFIVMDRLQAAGFSRETAT
                     SLLGQLKGMAGAFINLPQVFTIALAASLVPAISESIAKSDFDGASRKSELAIRLSLLI
                     GLPAAFGLFTLADPIMKLMYPAEPATLGMVLRYLSTAVIFLTLVQTMTGILQGMGKER
                     IPVISLIIGALVKFAVSYTLTSIPVINIRGAAIGTVAGYAATAFINLSAIVKHQKSSM
                     RISKIVVKPVIATGAMMAAAYLSYRMLFGAIGRNSVATLISVAFAALVYGVALILIKG
                     IATEELEMAPGGKRLSSILKKKGLL"
     gene            complement(26611..27168)
                     /gene="spoVT"
                     /locus_tag="PKI50_07795"
     CDS             complement(26611..27168)
                     /gene="spoVT"
                     /locus_tag="PKI50_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HNT03638.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDREGEV
                     ILKKYSPIGELSDFAKEYVESLHSSMEHITCIADRDTIIAVAGAPKKELLDKRVSPSL
                     ERIMEDKTTVVLSLTGENKMINISADEEGTPKYTAGVVAPIITQGDSIGAVLLLSKDP
                     NTQMGELEVKLSETAASFLGKQMEQ"
BASE COUNT         6783 a         7045 c         4726 g         8807 t
ORIGIN      
        1 acaatatatg aagaatgcga cgcgagacat ggggacgggg acacggggac gtttccccga
       61 aacgtcccca tgtccccgtc gcattattct tcagatgtga aaagggagtg ccgcaacact
      121 ccctttcact aagatgatat atgcttttgt aaataatcca aatactgcga taatatatcc
      181 ttcacttggc tgcaggtggt cagcttaaat atttccgcct ttactttggc ggaacccggc
      241 atccccttaa ggtaccaggc tgcatgttta cgcatttcct ttactcctgt tccttctccc
      301 ttaagctcag tgaccatatc catatgctcc atgattgtca gaattctttg ctcaaagtca
      361 ggctccggca gcaattcacc agttttaaga tactctgctg tcctctcgaa tatccacggg
      421 ttcccttggg caccccttcc aatcattacc gcgtcacaac ctgtttcttc gagcattctt
      481 cctgcatcct ggggtgttat tacatcaccg tttccgatta tcggtatatc aacggcatct
      541 ttgactcgtt ttattatatt ccagtcggca gtgccgctgt agaactgctc tcttgtcctg
      601 ccatggacag ttatcgccgc agcaccttcc atagcggcaa tggatgctac ttctactgca
      661 ttgactgatt cctcatccca tcccttcctg atctttatgg tgacgggcac tttgacagcc
      721 gcaactgtgc ttcttactat ttctcccaca agcaccggac ttttcattag cgcacttccg
      781 tcaccgtttc tgactatctt aggtgtggga caccccatat ttatgtctat tattgccgct
      841 ccgccggctt ctgcctgcct tgcagcctct gccactatat ccggctcgct tccgaaaacc
      901 tgtgctgctg caggtttttc ttcctctgct atctctaaca gcacagcggt attgtcactt
      961 ttataatgca gccccttggc actgaccatt tccgtatagg taaggccgca gccctgcctt
     1021 ttgcacagaa ttctgaaggc catgtctgta actcctgcca taggagctaa aaaaacgtta
     1081 ttctttagct ttacattccc tatctgcata acgcccacta tcattccctg tttctatcat
     1141 atattattct caaaccctca agagtaatca agccattcac tatatcaatg gtttcggatt
     1201 ctgaagctat taacttcgaa agtcctccgg ttgctattac ctttgtattc atgtccccaa
     1261 gctccttttt catacgcctt acaatataat ccacctgtcc cacatatccg tatattaccc
     1321 ccgactgtat gctgtttatt gtgttcttgc atattacagt actgggcttt gcaagctcaa
     1381 ccctgggcag tttggcagtc ctttcaaaaa gtgcatccat agatattctg ataccggggg
     1441 atattgcacc tccaagatat tctccgtttt ttgacactgc gcaaaatgtg gtcgccgtac
     1501 caaagtcaac aattataagg ggcccgccat aaagctcgta tgcggcaacg gcattgacta
     1561 tcctgtcggt tcccacttcc ttgggattat catattttac gttcatgcct gtctttacac
     1621 ccggtcccac aaccagcggc tccctgttga aatatttcct tgtcatgtgt tcaagggtat
     1681 acatgatggg aggtacaaca gagcatatca ccactgcctc aatatcagaa aatttgtgtc
     1741 cctgtaattc gaacagctgg tttattatca ttccgtattc gtcagaggtc ttttccgcat
     1801 cggtttttac tctccaaaaa ctgacaagct tcctgtcatc atatattcct aatacaatat
     1861 tggtatttcc aacgtctata acaagtatca tataaatcct cccatatcta ttggttatta
     1921 tcctgcataa agctgcaggg caccagttcc gcattaattt tagcaccgct tcactacttt
     1981 ttcaacaaat atgttctgcc caataaacaa cctgcatcaa actcatatat atccatgcat
     2041 gccccttatc gaaacctctc cggacattat ctccctgtat gaaccgtctg caagccttac
     2101 aacaaggtgg ccttccttgg atatatccac cgccagccct ttaatttcct cctttttgct
     2161 tatcagcctt acttcttttc ccaataccgc agacttactt ttataatctt ccagaaaggc
     2221 cttgatgctt ccatctcttg taaaaaccct gtaaatctgc tcaaactcga acagcacgga
     2281 agctgccagg gcttttctgg atacaggcat gccgctttca attcttaccg atgtggcaat
     2341 gcttgatact tcctgaccga agtcatcatt atccatattt gcatttattc caattcctat
     2401 aataacatat tttatctcat cttcctccgc actcatttcg gtaagtatcc cgcaaagctt
     2461 tttgcctcct gcgactatgt cgttgggcca ttttatttct gcatccagcc ggcagcagct
     2521 tcggattgca ttagtaacag caagggcggc gacaattgac agctttggtg catcggaagg
     2581 caatatgtca ggcttcagca taagggtcat ccaaattccc ttacccttag tggaaaccca
     2641 atgccttcca agccttcccc ttccgccggt ctgttcatca gctataacta cggtaccttc
     2701 ccggaataaa ccttcagctt ttttctttgc atatgtattg gtagagtcaa tactgtcgag
     2761 ataaattata ttcctgccca tgaactcagt atgtaagtaa ggttcaagtt cgcttgatga
     2821 caggatatca gggctcccta ccagcttata cccgctgttg gtatgggatt ctatgctgta
     2881 gccttcactg cgaagcttgc ttatatgctt ccaaactgca gtacgggata cccccagcct
     2941 tctgcttatc tcttcgccgg ataccggctg accgctattt gcccttaatt cttcaagtat
     3001 cctgtttttc atactttcac ctcattgccc gtccatgctc aaagcttggc tcgtgctctt
     3061 cgagtcttat atattatatt ctactataac ttcaataaaa aatcagacaa tttcttgtct
     3121 gattttatac attgtctgct attatcccag catggccaga agcactccgg ctgcaactgc
     3181 agagcctata actccggcta cgtttggtcc cattgcatgc atgagaaggt aattgcttgg
     3241 atttgcctcc tgccccacct tctgtgatac ccttgccgcc attggcactg ccgatactcc
     3301 tgcggagcct atcagtggat ttaccttccc gcctgacatt ttgcacatta tctttccgaa
     3361 tattactccg ccggctgttc ccatggagaa tgccgcaagc cccaatgcga ttattgccag
     3421 cgtctgcgcc cttaagaata tctcaccgtt tgcagttgca cctaccgcaa ctccaaggaa
     3481 tattactact atattcataa gctcattctg ggctgttttg gaaagcctgt tcaatacact
     3541 ctgcagatcg actgcttccc taagcaggtt gccgaacata agaagcccga taagcgctgc
     3601 tgccgatgga agtattatcg atactaccaa ggtacagagt attggaaata ctatcttttc
     3661 aatctttgat accggcctca gctgttccat tactatagct ctttcctcag gagttgtcag
     3721 tgctttcatt ataggaggct ggattagcgg taccagagcc atgtacgaat atgcagctac
     3781 tgctatcggt cccaaaagat gcggggccag ttttgccgta aggtaaatgg ctgtcggtcc
     3841 gtcagctccc cctattatgc ctatggagcc tgcttcctgg agggtaaagg ataatcccgg
     3901 aacaagattc aatagtattg ctccgataaa ggttgtaaat atacccaact gtgctgctgc
     3961 tcccagaagt atgctcctgg gatttgctat aagcgggccg aagtctgtca ttgcacctac
     4021 tccgaagaat atcagcggcg ggtatattcc cagcttgaca ccctggtaca tatagtacag
     4081 tactcctccc gcctgtttca tttgatgcac atgctcccct gttaccggat ctgttgcgat
     4141 ttcatataca ggacctgcca taagccctgt cacgggaagg ttcgccagca gcatgccaaa
     4201 tgctatgggc accagcagaa gcggttcaaa tcccttcttc aatgcaagat acataagcac
     4261 gcaggataaa caaagcatga ctgcctgctg ccaggttatt gccgcgaatc ccatactata
     4321 ataaaagttt aataattcat ctaataatga cacccggatt cccccaatca ttctttaaac
     4381 tttgcaggaa ttttcttaat tcccctgttt caataacact aggcaaatgt aatcaatacc
     4441 tgtcctgtgc ttacagaatc cccctggctt acagctactg atgctactgt gccgtctgcc
     4501 cctgccatta tctcgttttc catcttcatt gcctcaagta tgcaaagaac ttgtcccttt
     4561 tttaccgcat ctcctgcttt tacattaatg ctgagtatcg tacccggcat cggtgctgtt
     4621 actgtatttg cccctgcagg tgctgctgcc ggtgctgccg caggcgcagg tttcggcgct
     4681 gctgcaggtg ctgccgcagg ctttggcgct gccggccttg gtgctgttac cggtgctgtt
     4741 gcctgtgctg caccttcctt tatttcttct acttcaacct catagctatt tccattaaca
     4801 tttatcataa attttctcat gaaattttcc tccttaatta atatttagaa cctgacagcc
     4861 atctgttccg tcctgcttgc cttaccccag gccggagtca gatcctcaac ccgtctgata
     4921 tttcttactg caatattgct gttccctccc agacaggata ttaccgcagc ggttattaca
     4981 gctgccagct gatcgtctga agcttcctcc gcctcattca cttgcggttc atctggctca
     5041 gccggtttta ctgcagaaac ctgagcaggt tcctggactg ctttcttctc cctgtgaaat
     5101 attatttcca tggctttcag catatactgc agaaggatca atactaaaaa tactatgccc
     5161 attccaaaaa gggtcactat caaggcatat gtaaaggtat cgctgaaatt agtcattatc
     5221 tcaccccatc cttcttatta tacaggcagg ttgccatgct ttttggcggg cctgctttct
     5281 ctcttgcttg caagcatttc aaatgcatta gctaacctcg gccttgactc ggagggttct
     5341 atgaccgaat caacataacc tctttttgct gctatatatg gtgtggcaaa cttgtctttg
     5401 tactcctgta tcttttctgc ccgggtctgt acggggtctt ctgccccttc tatatccttc
     5461 ttgaatatta tattggctgc tccgtcaggc cccataaccg cgatttcagc tgaaggccat
     5521 gcaaatacca tatctgctcc aagatccttg ctgcacatgg caagatatgc ccctccgtaa
     5581 gcttttctta ctatcagtgt aacctttgga actgtggctt ccgaatatgc ataaagcatc
     5641 ttggctccgt gccttatgat ccctccgtat tcctggtttg ttcccggaag gaagcccggt
     5701 acgtctacca gattcagtat cggaatattg aaggcatcgc aggttcttat gaatcttcca
     5761 gccttatccg aagcgtttat atccagacag cccgccagaa ccttgggctg atttgctatt
     5821 attcctatgc ttgctccgtt tattcgggca taagcagtta ttatattctg tgcatagtac
     5881 tcctgcactt ccatgaaatc tccgttatcc acaattgctc ttatgatatc cctcatatcg
     5941 taaggcttgt tgggattatc cggtactatt tcattcaatt gctcttcgat tctatttata
     6001 tcatcatcgg tttcaaatat cggcgcagtc tccagattgt ttgaaggcag aaagctcaaa
     6061 agcctcttta tgtcttggat gcaggcctta tcatcagagc ttatgaaatg tgccactccg
     6121 cttgttctgt tgtgggtcat ggcacctccc agttcctccg gggagacctc ttcccctgta
     6181 actgccttta tgacctgtgg ccctgttatg aacatctggc ttgtcttgtc caccatgaat
     6241 gtaaaatctg taagggcagg agagtaaacc gctccccctg cacaggggcc cataattacc
     6301 gatatctgcg ggacaacccc cgatgcaatg gtattcctgt agaatatgtt cccgtatccc
     6361 gacaaggcat caacaccttc ctgtatcctt gctccgccgg aatcgttcat tcctactacc
     6421 ggggctccca ttctgagagc caggtccaat accttgcata ttttctttgc atgcatttca
     6481 ccgagggaac cgcctataac ggtgaaatcc tgtgcataag catacaccag tcttccgtct
     6541 acagtaccgt agcctgtgac aactccttca gcaggagcat ccactgcttc cataccgaat
     6601 tcgctgcatc tgtgcttcac aaatgcatcc agttcaacaa aactgtccgg atccatcagc
     6661 aggttgattc tctcccttgc ggtaagcttt cctgctttgt gctgcttttc tatcctgtcg
     6721 gctcctcccg ctgctgtgat tttatctagt ctgctgtgta aatcgttgat tttatctatt
     6781 gacatagaat ctcctccact ttcttaatgc tcttactttc tctcacaaag ctctattaaa
     6841 acaccgccgg tactcttggg atgaaggaat gctattttcg caccgccggc cccatatctc
     6901 ggtttttcat caatgagcct catccctttt gctttcatct cttccagtgc tgcctcaata
     6961 tcatccactc taaaggctat atgctgtatc ccctggcctt tagcttcgat aaacttcgcg
     7021 attggccctt cggccgatgt cgattccaat agctctacct cagtatcccc taccggcagg
     7081 aatgccactt ttactttttg ctcaggaact acttcagttc catggagttc cagtcccagt
     7141 acatccctgt aaagcttcac cgcttcatca agattactga ctgctatacc tatatggtct
     7201 atcttgctta ccaattgaat acctcctttc atctattatc tttaatactt cttcgaataa
     7261 tatatatata atacctttaa ttgttccttt ggttatttgt aaattcatcc attaatctat
     7321 ctgcagcggt gtaagggtcc atctgcttat ccattacctt ctgcgccaat tcgttaataa
     7381 tgttggtatt ctcacttata ctatacaggg ttttcataac ttcgtccctg aaaatatcca
     7441 ttatctctgt tcttaacctg ttgagttttt tctgcttgta gctcccgctt ttgttctcat
     7501 attcccagtg cttttcaata agttccagaa gcttttcaat gcctttgttg tctcttgcaa
     7561 ttatattcaa aaccgggggc ctgtattctt cattccggtt gaaatccagc atggcttcta
     7621 tttctgcagc agtacgttca gcatcatccc tgtcggattt atttactgca aaaatatctc
     7681 caatttccat aatgcccgcc ttgatagctt gtatgtcatc tcccagcccc ggaaccatga
     7741 ccattataac ggtatccgca agcttcacaa tatcaatttc agactgtcca acgccaactg
     7801 tttcaataaa tatgtagtcc attccaaaaa tatccagaac cttgacagct ccatggactg
     7861 cctttgacag gcctcccaat gctccccttg tacccatgct tcttataaaa actccggggt
     7921 cattattcag gtcagccatc cttactctgt ctccaagaat tgccccgcct gtaaaagggc
     7981 ttgtaggatc aacagcaact atacccacct gtttgccctc tttccgaagc tgcttggcaa
     8041 gcttatcggt tacagtactc ttgccgactc ccggcggccc ggtaatacct ataatcctgg
     8101 catggcctgt catgttgtag cactctttga ttatttgaac cgcttccggt tctccgttct
     8161 ccatcattga tatcagtctt gcggcagccc tcttgtcatt attcagcaag ccggccttta
     8221 tatccatata tatctcccgt tactttcttt taatattgtt tttaataaaa tcaatcgtta
     8281 cggatgtagg agttcccggt gtaaatattc ctgcgattcc tgcttctata agcccgggaa
     8341 tatcatcctc cggtattacg cctccgccta ctaccagaat atcatctgca cccctctcct
     8401 taagaagatt gacaaccttc gggaaaaggt gattgtgcgc tccggataat atactcattg
     8461 caacgcaatc cgcatcctcc tgtatagcag tctcagctat ctgctcaggg gtctgcctca
     8521 ggcctgtata tatgacctcc atacctgcat ctctaagtgc tcttgcgaca acctttgcac
     8581 ccctgtcatg accatcaagc cccggttttg caactacaac tctgattggt ctatccaaat
     8641 tcaaaacctc ccaacatata tcttcaaatt tattggctaa aatttttttg ccttattcgt
     8701 ctatgagcct atacagtttt gggtctcgcg gttcgtggct cacggctcgt gtttcaagga
     8761 aaacctccga agccctgctt gcacccacgt gcctcgtacc acgcgcctcg cgccaattta
     8821 cagtactatc gactgctgat actctccgaa taccttcctc agtaccccgc agatttcgcc
     8881 gagtgtagca tacgctttta ccgcatccag aataaaaggc ataaggttgt cttcgccctt
     8941 tgccgcattt cccagtttct ccaatgcggc attcacttta gtattatccc ttatttttcg
     9001 gagttcggca agtttctttt gttgaagctc tccaactttt gggtcaacct ttaagagccc
     9061 tttcggtgcc gactccttaa tctggaattt gttcacccct actactattc tctctcctgc
     9121 ttctatctcc ttctggtact tataggcgct gtcctgtatc tctttttgta tgtatccctt
     9181 ttctatagcc tttgcagctc ctccaatctc atctatcttg ttcagatatt caactgcctt
     9241 ctgttctatt tggtctgtca ggctctctat gtaatatgaa ccggccagag ggtctgctgt
     9301 ctctgtaaca ccgctttcat aagctacaat ctgctgtgtc cttaatgcaa tcctcaccga
     9361 atcctctgtt ggaagagcaa gagcttcatc ccttgaattt gtgtgcaggg attgggttcc
     9421 tcccaataca gcagccaatg tctgcatagc aaccctcaca acgttgttgt cgggctgctg
     9481 ggcggtaaga gtacaaccgg ctgtctgggt atggaacctt aacatcattg acttgggatc
     9541 cccggctttg aacctctcct tcattatctt ggcccagagt cttctggccg ctctgaattt
     9601 ggccacctct tccagaatct cattatgaga gttgaagaag aacgacagtc tgggtccgaa
     9661 ttcgtccaca tccaggcctg cttttaccgc agcatcaaca tatgcaatcc cgtctgcaag
     9721 ggtaaatgcc acttcctgtg ctgctgtcga acccgcttct ctgatgtgat atccgcttat
     9781 tgatattgtg ttccattttg gaacgtgttt tgaacagtat tcaaaaatat tggttataag
     9841 cctcatggaa ggctctaccg ggaaaatata tgttcctctt gctatgtact ctttcagaat
     9901 atcattctgt attgtgccag agagctggtc tgatgaaacc ccctgctttt ccgcaactgc
     9961 tatatacatt gccagcagaa ctgaagcagg agcatttgtt gtcattgatg tgcttacctt
    10021 atcaagaggg ataccgtcaa aaagtatttc catatccttg agggaatcta ttgcaactcc
    10081 gacctttcct acctcacctt cagaaagcgg gtggtcggaa tcataaccta tctgtgtcgg
    10141 gaggtcaaaa gccacactaa gccctgtctg tccctgctcc aaaagatatt tatacctctt
    10201 gttggattcc tctgcagttg caaagcctgc atactgtctc atggtccaaa actttcccct
    10261 gtacatggta ggctgtactc ctcttgtata gggatattcc ccgggatttg acaaatccct
    10321 gtcataatcc aggttttcta tgtctgcagg tgtataagcc ctctttaccg caatgcctga
    10381 agacgtctca aattccttct tcctttcagg ccgtttggca actgcttttg tgacagtgtt
    10441 ttcccactct tcgtgggcct ttttcagctg ttccagcttc tccttattga acatatttat
    10501 cctcctttta ttatttgagc atatccgatg gaaaatatca acaaaggata ttgcagcctg
    10561 tcggtcaata tcatattgtg aaataaaaaa ttcattatat tatcattttg caagttttcc
    10621 ttctttatta atattaccat attatttatt aaattgtgta gtatttttag ataattttta
    10681 agaaattctt tgttaaaaaa tagacagtgt acatgctgtg aaaaagcctt atcattacaa
    10741 gaccggttaa agcatctgct tatgtattaa agcacttttg aagttttttg agcggctctt
    10801 tcaagaaatt tagaaagctt gattatatac ctttcatgga tgccttcccc gattttttcc
    10861 tgctcgtcat aggcttcaac cttgaaggtc agcttcttgc cttctatgcc aatgagttca
    10921 gcggcggcag tgactttcat gccaacaggg gttgcagcca gatgtttcac attcaaactg
    10981 agtcctacag tggcagagtc ttctcccaag tgcggatcca ctgcattaag tgccgcgttt
    11041 tccattaatg ctaccatcat aggggttgca aaaaccctga ctcctccgct tccgaattta
    11101 actgcggtat catctacact aacggttttt cccacttggg ctgttattcc gatcttaaga
    11161 ttattatcca atatgatcac actcctatat tatttagctt gtgccaaaat ttaaagccta
    11221 gcccttagaa ggctaagctt taatattcta tgcgctggca aacacttctt caaattcagc
    11281 ttgatcaagc ttttctctct ccaggagagc ctgggcaact ccatgaagct tgctgatatt
    11341 ttcttttaag agtgtttccg tcttatgata agcagcatct atgaccttcc ttatttcctt
    11401 gtcaattatt gctgccacct cttcgctgta gttccttgtc cttgtaagat cctttccgat
    11461 aaatacctcg tcatgctccg atccaaaggt cattgggcca agactatcgc tcatgccgta
    11521 ttcggttatc attttccttg cgatgccggt ggctctctct atatcattct ttgctcccgt
    11581 acttacgtcg tcaagcacaa gcttttcagc aacccttcct ccaagaaggc ttactatttg
    11641 ctcttccagt tcagacttag acatgtagaa tttatcctgc tgcggcaaag atattgtata
    11701 acccccggcc cttcctctcg ggataatgga tatctgatgt acaggatctg catttggaag
    11761 cagcttttgt actactgcat ggcctgcttc atggtaggct gtaagcttct tgtcatgctc
    11821 actgactacc ttactcttct tttcaggccc tgcaatcact cgtattgtgg cctcgtcaag
    11881 gtctattact tcaatctttt tcttgccttt tcttgcagcc aacagcgccg cttcattcat
    11941 tacattttca agatctgcag gagtaaagcc ggtagtgcct ttggccaggg tttccatatt
    12001 aacatccaat gaaatcggct tattcctcgc atgtatcttc aatatctctt ctcttgcttt
    12061 aacatcgggt atcccaacgg taatctgtct gtcaaaacgt cccggcctca gaagcgccgg
    12121 atccaatata tcaggcctgt tagtcgcagc aatgatgata attccctcat taacactgaa
    12181 tccatccatc tcaacaagca gctggtttaa ggtctgctct ctttcgtcat gccctcctcc
    12241 aaggcctgca cctctgtgtc tgcccactgc atcaatttcg tcaatgaaaa ttatgcaggg
    12301 tgagtttttt ttcgcctgtt caaaaagatc ccttactctg gatgcaccga caccaacaaa
    12361 catttccaca aaatcggaac cgcttatggt aaagaacggt actcccgctt caccggcaac
    12421 tgccctggca agatacgtct tacctgttcc cggaggccct acaagaagaa tgcctttcgg
    12481 tattcttgct cccagttcaa taaaccttct gggctgcttc aggaagtcaa caatctctcc
    12541 cagttcttcc ttttcctcat ctgcgccagc tacatcggca aaggttattt tctttttgtc
    12601 ttcggtatgc agctttgccc ggctctttcc gaaggacata accctgctgc ctcccccctg
    12661 ggactgctga gtaagcacaa agaagaatac tatgaatata acaatcagca ctattgacgg
    12721 cagtattgaa aaccaagccg ccgctgctgt cttgatttca tatttaacat ccagcctgtc
    12781 tctctctata taatcctgaa ccagttcatt aaactcactg cttggaacaa ttgcttcaaa
    12841 acgccttgta tttgaaatct tgcctgtaac aacagacata ccgttgttat ccgatactgt
    12901 gacttctttt atacgtccgt tttctatttc tgtaaccaac tgagtatagg acagtttatc
    12961 tttagcaggt tcagcattct gaattatcaa tacaaacata tatattatga tgataatcaa
    13021 tatataaaag cttatacctc ttaagaattt cttcaacaat aatcctcctt tctatataag
    13081 tcaacaaaca acacagatat tttagcatac caaaacttaa attacaatat cgcagcaggc
    13141 aagccaattg cctatcatac acataagaag agaaatgcgc aataactgaa gtttgcataa
    13201 ccctccgcaa cgcggggaca tggggacgtt tcgcgtgtcc cactggaagt gggacagggg
    13261 aaacgtcccc atgtccccgt cgcattattc tactttttct catatacctc cggtttaagc
    13321 acacatacaa aaggaagatt cctgtaattc tccccataat ccaggccgta gcctacgaca
    13381 aactcatcgg gaatcttgaa ccctatatat tgaatttcta cttgcgcctt tctcctgtca
    13441 ggcttgtcaa gaaggctgca aaccttgaca ctcttaggat tccttgcctt gaaattttct
    13501 acgaggtaac tcagcgtaag gcccgtatcc acaatatctt ctacaataag aacatccttg
    13561 ccctctatct ctctttccaa atcctttact attcttacaa cccctgagga tttggttgta
    13621 gcaccatagc tggataccac catgaaatcc aatgtcagag gcagatctat agttcttacc
    13681 aaatcagaaa ggaacagcat gcagcccttc agtatcccca ctaccagcag ttcttttccc
    13741 ttataatctt ctgcaagctg cttgcctagt tccttaactc gttttgctat ctcctcttca
    13801 cttatcaata tacttttcat atcttccagc accgtattac ccccttatgt attttatcaa
    13861 aacagcatct gaagtatcat cagagatttt atacttttcg ctcaatcttc ccccaacaat
    13921 ccagacaatc tcatgtccgt ctgcaataag ctcaaggtca tctcttttct ctctcggaac
    13981 tttctcatct atcaggtatt ccttaagctt cttggttcct ttcatcccta tcggagcaaa
    14041 catgtctccg gcctgccggc atcttaagac aagctctccc tttatcttta aaggatcaat
    14101 gtaaatagtg tctttttgcc ctttaagctc tgcaatttcc tttgccttta tcctttgcac
    14161 cgttatacgg ccgttatccg tatcaacact attgtcataa tcatatttta acttataata
    14221 acacttttta tcagccttca caatattttt tgccagtaca agcttatcgt aggatctgaa
    14281 tgccttaagc ttatggggaa gcatcacggc agctcccgtg gattgatgtg caatctccag
    14341 tactttctca aaatgtttga attcaaaccc gttcaaagta tttaaaagcc gctctgcaca
    14401 ttgcctgatt atccttctct gcaatgcttt atggagggca atgaattttg tcacttctat
    14461 aaccactctg tcatcggaat cctcagcaac ctctttgaac ttttcccttg cttctgtctc
    14521 aagaaaatca ttctcatcct ttatcagtcc ggaaaatctg cttaaagcca ttttaatatt
    14581 tggattgaaa ttcttctcca tatatggaat cagttcaagc cttatcttgt tcctgttata
    14641 tacagactcc aggttggtct tgtcaattct cgggtttaag ttatagaaag cacagtaatc
    14701 ttctatgctg agcctgtctg tttcaagcag tgggcgtatg atgtttcccc ttacggcctc
    14761 gattcccttt agtccttcta ttccgcttcc ccgcatgaat ctcatcaata tcgtctcaat
    14821 atgatcgttc agattatgcg caactgcaat cttattgccg cttacctctt cacatacccg
    14881 gcggaagagc ctgtatctga tctccctccc cgcttcctcg ggagaaagcc cggtttcctt
    14941 tatatatgcc ggcacgtcaa aaacctctat aaataccggg atatcccatt ctctgcagat
    15001 tttttctacg aattctgcat cgctgtctgc tgccttcccc ctgaacttat ggttcagatg
    15061 cgccgcaaaa atgctgcagc cgtattccct gcacagcccc ttcagcacat gaagcaggca
    15121 tagtgaatcc gggcctcctg atacaccgat tacaattttg tctccgggct tcaccatatc
    15181 atattttgct attgtctttc tgacattttc aataaagcct gtcataaatc caccctcaat
    15241 atgtttatgt attaatgcta ccatacagcc gcaacattta aaagcaaaaa ctaagaggac
    15301 atggggacgt ttctccgagg acacggggac gtttctcctg tcccgctttc agcgggacag
    15361 gagaaacgtc cccgtgtcct cggagaaacg tccccgtgtc ctcggagaaa cgtccccgtg
    15421 tcctcggaga aacgtccccg tgtcctctta tatttcaata gtacaaggtt ctatgcattg
    15481 tatacttcat aatactgatt cataagctcg caagctttta gtattgcagc tcctgcctgc
    15541 ccgtcaccgc tgcagcatat tagttccgga ttccggttca ataggcttat gtcgccggca
    15601 aattcacata tggtgtcatg tattccggca gtttcacccg aacaatctat tactgctata
    15661 ctgctgcagc ctttccttat cctgatactc tcaagcagtc ccgctgcact tttctccgca
    15721 atagtaaaac cgcctttaat gattctgccg gcatcgataa caacaataca tttcaggttg
    15781 atgtttcccc cgtatttcct aatgaaattc attatcttcg ttctcgattc gggctccaga
    15841 tccgggatac cctctccgat aattgcctta agctgccggt ctacaatccg ttctattgtt
    15901 tttacggatg tatcaatggc aatctccttt actgcatatt tcaaatcttc tgccttcgaa
    15961 taataccata gccctccccc tgcctctgca atcttctttg cctcttccgc ttcactttca
    16021 ctgccggcct caggatcaac aatacctatt gcgcttacga caataccttt atcatatgct
    16081 tcctttgcag cttcaacaag actgattccc gaaccggatt tgccgtctgt tacaataatt
    16141 atctgtttga tttcaccgct gtcaatccat gcatccataa aaatttccct ttcctgcaaa
    16201 tgtacgaatc ttacaactaa gtatattcat tattttgcca aaaaaggatg ccattataca
    16261 aagaaatgct ttacattgcc tcccacacct ttgctgccat tacagtcata tcatcgggag
    16321 cctctccgcc gtttgcctgc aggcagctct ggagtatttc ctccgccagc ctctggggat
    16381 tcctggtatc cacatccctt atcaattctg ccagccactt ttccttatca acaatttccc
    16441 tgttgcagtc caataccccg tcagtaacca ttaccacaaa gtcaccatcc ttaagcttct
    16501 tcctgacaag ctccacatca attctgctca gtattccgac cggcaatgta ccagtcttga
    16561 taatctgcac tccctcttca tttttgatga atgtagatac agcacctacc ttgatgaatt
    16621 ccacctcccc gttgtactgg ttaataactg tcatgtccac agtcgaatat gtttcgtcat
    16681 tggacttcag aagcatcagc gaattgatgg ccttcaaagc cacatccttg tcaaagcccg
    16741 catataggaa tttttcaaga agggttattg ttgtgctgct ttccagtgct gcttttgggc
    16801 ccgtccccat gccgtcgctc agggcaagca tgaatttgcc gtccttcagt tctataaatg
    16861 aataattgtc tccggaaatt gctcccgact tattcatcct tgcaatgccc gtagctattt
    16921 gatacctctg tgcttctttc agtttgatgc tgcatatgct gccgtgtatc atatcgcagg
    16981 tcatctccct tttgttgaat ttctttccgg ttactctgtt tacaatcgga atgatgttct
    17041 tgatgcattc ccgcttccct ccgcagctgt ctactcttat atctacatcc attgtgtttc
    17101 ccgttcttgc cactatcaca tcttttactc tcaccgcatc attgtccaat cctaccataa
    17161 tcagttcctc catatccctg tcaaaatcaa catccatatc tatccttaaa gctaggtcgg
    17221 ataccactgc ggatacctcc ttcaactgat cacttacaag caatctgctt tcatttattt
    17281 ttttcttcca gccaagcccg tttctgtaca tatcatagta atatttgagg ttttcaataa
    17341 tttcctccgg cttcaggcag cgttccttga agcctttata gcagccgtcc tttttgagtt
    17401 tacccccgtt ttcaataata gtcagcaagt tgaaaagata ctgatagcta ttgtaaaagt
    17461 tcctcttcca gcagctgcct gccaatccgc agtctgaaca tgtcttcccg actacctggt
    17521 ctattatctg cgctgcgtcg gtatgggaaa aatagctcct gtcctgtatg gtatccttca
    17581 atgtatcagc cagtgattta tatactcccg caaactcaga gagcctttct atggtattct
    17641 cctttatcct catgctgtaa gacttttctt tgagatactc tcctgcaatg gatagtttgt
    17701 atcctgacag ctttttcatg tagttttggg gaatacagac aaacagaaag gaagcaatca
    17761 atatttcata aaaccttatc aatacctctg tagagccgtt tatataaaag gtcatcagtg
    17821 cgttgcttat aatgaagccc agggggcagc catacctgcc gaggttcttc aatattccgc
    17881 acatcatcgc acaaaggccg taaacaccta tggcggaagg gaggatagag cctgacaatg
    17941 cctgaactat tcccatgatg attcccactg ccgcacccgt tccgacattg ccgagataag
    18001 caaaaatcat aataatgaat atgacggcaa tgttttttac tgaaaaaccc gcaatatata
    18061 tctctgtccc tacgaaacag aaagctgcca ttaccgcaac agcgacaacc tcttcggagg
    18121 acagcagcct tctgttcttg taattcttta tgacaggtaa ggaacgatca taaatgtata
    18181 tgagtatggc tattaaaatg gattctataa tcaacatcaa aagatcatag agataataat
    18241 tctgaacata aaaaatgaaa taccccgctg caaggttaat caaggctgca gcagctgata
    18301 tcaccagttt cctgttcact ttcatcttac gggttgctgc gtatggaatt atcaagacaa
    18361 tagctgcaat aatatatttc aaaagtatcc ccttatctcc gacgctcagc actccaagca
    18421 atatgcttgt ccccatcaca aacagattca gcttctgaac catagccgcg gcaaaaaaag
    18481 cagtgcagaa agggttaaga aaatccagaa taaccgctct tcccaataac agcccgaagg
    18541 cactaagtat catatacatt ctgaagctgg tattcatgaa aaaatctatg atatgcctta
    18601 tccggccgcg tttgtttgta acactttgat tagatactgt tgccacttca accaccatcc
    18661 aataattctt ctaaacgtcg tataaagatt ctaacacaag gggtttgaat aatttgtcag
    18721 gttaggtgct tgaaaatgga aaactttttg acaatggaga ggtttttttg acatagggtg
    18781 tgcagtatat ggaatatgag acaaaaacta aaaaactcag acccaagtct gagtgttttg
    18841 gcgttttggt agcggcggtc agattcgaac caacgaccgt tcgggtatga accgaatgct
    18901 ctagccaact gagctacgcc gccatgtata aaaaattggt tgcgggggag ggacttgaac
    18961 ccacgacctc cgggttatga gcccgacgag ctaccaactg ctctaccccg cgacgtttgt
    19021 ggtgccgaag accggaatcg aaccggtacg gtctttaagg accgcaggat tttaagtcct
    19081 gtgcgtctgc cagttccgcc acttcggctt aactgccaat atcgataccg caaattaacg
    19141 gcaagtatta ttatatcgtc tttgactata aacgtcaata cctaattatt gacatatcac
    19201 ctttgccact actcagccgg cttatttgct caatacatat ttttcaataa tcccttcgag
    19261 attgtcattt tcgctgacga ccagactgtc tcttttcagt ccttcgataa tatgctgcaa
    19321 tataaatatg ccggagggca tcacatcagc tctttccttt tgaagccccc taacattcca
    19381 gcgctcctca acagacatgt ccttaagctt gttaaatatg ctctctatat attcagaatc
    19441 tacaatcgta ttatgcactg tttccggatc atatatgctc atattatgga acattgctgc
    19501 tattgtagtg gcagtgccgc cgatagcgac taccttatct attctttttt tgccaaggtg
    19561 ctccaagggc tctttaaaaa tctcattaag cctgtttttc atattttcaa catcttcatt
    19621 tctgatagga ttgccggtaa caaaatgctc tgtcatccta accgctcctg cgggaatact
    19681 gactttgtag tctgtctttc ccttgctgcc aagtaccagc tcagtgcttc ctccgccaat
    19741 gtctattacc agcacagttt cctgcgggtt ctgaatctca ctcattactc ctctgagtcc
    19801 aagctccgcc tcttcatctc cgcttattat cataacatcg actcctgcct gacttttggc
    19861 atcggcaaca aaagctcctc cgttcaatgc gtcgcgaaca gcactggttg ctattgcgta
    19921 tacctcttca gctccatatc tgtcggcttt attcctaaaa tatttcagag cttcaatatt
    19981 ccttgcaaag gatttttctg taatcatccc cgtctttgaa acatcctcgc ctattctggt
    20041 aatcatgagt tccttttcct tatttgcaat gaaattgcct tcaattttgc aaagcatcaa
    20101 cctcattgag tttgtgccaa tatcaataac cgctattctt ttcatttgct tctcctttta
    20161 ttattccgtt catgccaaat ttataaacaa taaactgcca gacttgccgg cagttattta
    20221 cgcttattat tatatatgat taaataattg cagtttgaat taagccctat tgttaagttg
    20281 attatccttt ttgcagccca tcgtttttcc tgtacccgcc attacgtttt gagtcagtat
    20341 tttttttaag ctgttgaagt ttttcgtcgc tttccttcag gaacttcgaa agccggtctt
    20401 caaatgaaat accctgattt tttcttgctt cctgctccca gtctatttca ataggtctgt
    20461 tggtcttttt aatttcctgt gcctgtttta ttgaaagatt tatcttcccg tttttttcaa
    20521 cggaaagtac tttaaccatt accttatcct tttctttgat aaagtgtttt atatccttaa
    20581 cataagtatc tgcaatttct gatatatgga ccagacccat tttaccctcc ggcagctgaa
    20641 caaaggctcc aaagcttgta atgcttgaaa caataccttc aactacctta ccttcctcta
    20701 taggcatacg aaaaatactc ctccctcaat aacaatagaa ctgctatgga tataatatat
    20761 taaaaccctt ctgctgtcaa tattgtgcct gattctccct gttctgtata tttctatcag
    20821 ccaaggtgcg cagacttaga atagtgtcat tctttctcat cggaggattg gtctatatag
    20881 accttttccc ccggtttaac caagcccaat tgctccctgg ccaacttttc tatatactca
    20941 acagaagttg cattattaat catttccatc agcctgtcat tgtcatttct gatagcttct
    21001 attttagaga tttcatcctg ctctgacttt cttaggtcat aaattgtaag ctgctgattg
    21061 actattgtaa tggcagcata aactataaaa gcaatcatga atatattctt aagcctaagc
    21121 ttccgctttt tcatatacat cgcctcaaac ctttattaat atagtatgta caactttttc
    21181 acatattact ttaatttcta agcaaaatca aacacttcaa ctgattcagc aatgataaat
    21241 cttcttcacg gcatattcag cattattaat attcgacggt ggtcaggaaa atcctttatt
    21301 actgttttct tttctctctt ttcttttcac tataagccta tagtactttt ttgagtcata
    21361 tattacttgt ttcggcaatc cgcatacttt tcttaatctg atttttttct tccccaatat
    21421 acgtatcaag ctgcaacagc aactttttac aaatgaacag gccatcttta agggcagggc
    21481 caggatactg ccggttttca taaaaaaact gctgatactc ttgtataaca aatgaaacag
    21541 tcctgatgct atcctcccaa ggagcttttc atacaaatag aatccgacca gaaatccaat
    21601 aagtacataa cttcttattt ctccgtagct gctggagaaa atcgcccata ttacaactgc
    21661 gaataccgag aaaagaaaca gtatgtccca taaggaggta atcagtcttt cagttttttt
    21721 tcttcctctt ataaccctgt aaatatcata tagaattcct atgattattc cgccatataa
    21781 cgaatataaa aatacataac tctgaaattg aacggtttcc actgaataac ctccctattg
    21841 ctcaaaaaat ggaggaggcg caataagaaa ccagtaacac gagagacacg aaaatacacg
    21901 aaaaacacga aggaaaaata ttacgataca ttgagaatta tcaataatat cgtaagtatc
    21961 gaaaatcgct atttcatcga tgaattgtat aaatgttacc gtgtaatcgc gcagcgattc
    22021 cgtgaaatat cgtgtgttta gtgttacccg ttacctatgt cgcaattttc ttctgatact
    22081 cccctattta aaaatattgc ttaagaaact gccggttttt ttgatatcga ttttgtcgtt
    22141 atatacgcaa gaaattatgt aaccttcgat aaatagattg ccgtcatcaa cgttcagcct
    22201 gttaatgtgc aaatcagttc ctcttataac aaggcccccc gcctccgttt ccaatgaaac
    22261 ttcattttca ttaaaactgg ctacatctct gactccgttg ataataagcc tttctcgccc
    22321 ttcaatcgtt atactgtgag gttttgactt aatgagcttc ttttcttcca taaaaatccc
    22381 cccaccgatg gtatattgct tactagtatt tatatgcatg gagggtgtat ttatgaataa
    22441 gaagtgcgcc cttcggcgca cttcttatat aagcttatac atctctcttg ctgaagcttt
    22501 tgatacatta tccgatatgt ctgtaatctc cgcctttatt cttttttctc cgaagacaat
    22561 ctctattatg tctcccacct tcacttcagc acttggcttt gccagtttgc cattcagata
    22621 gaccttttca ccttcacagg cctccttggc taccgtcctt ctttttatca gcctggaatt
    22681 ctttaagtat ttatcaagtc tcaatcagtt cacatcctct ttataacata gaaatagtat
    22741 ggaaatgaaa aatcaggccc gtccggcctg attcctctaa aatatgtagt tattttttct
    22801 tatttacgag atccttaaga tccttgccag ctttgaatat cggagctttt gatgctggta
    22861 ttttgatctc ttctctggtt cttggatttc tgcctactct agcctttctt gctttaactt
    22921 caaaagttcc aaagccgact aactgaactt tatcaccttt tgcaagtgct tcttcaacac
    22981 tctcaacgag tgccttaaga gccttttcgg aatctttctt tgtcatagca cttttttcag
    23041 ccattttgct gataagatct gatttattca tcattttccc tccatttttt attttgtagg
    23101 aaatatttac tgtttatgga tattctacct cattcccgaa aatccttctt ttttttcatc
    23161 ttttttcatc tttttttgct aaaacttgct gttttgcttc gttccacagc atatccatct
    23221 cctgcaaact cattttatca agttttctgc cggattgcaa ggctgtttct tcaatatatt
    23281 tgaacctttg gataaatttt tccgttgtgc ttcttagggc aagctcaggc tgaaccttta
    23341 agaatcttga tacattaaca actgagaaca gcaaatcccc tatttcttcc tctattatag
    23401 ccttttctcc ggttttatat acttccttta actcttctgc ctcctcataa acctttgaaa
    23461 atgcttcttc tacttcatcc cagtcaaagc ctgccaacgc cgctttctgc tgaaccttgt
    23521 acgatctcat taatgccggc agtatgtcag gtatgtcttc aagagtcttt gtatatgtgt
    23581 ctatgccttt atactttcgt tttgatgctt cccaactctt taacgctccg ctttcactgg
    23641 tagcttttat atctccaaac acgtgaggat gccttattat cagcttattt actatgttgg
    23701 ttattacttc gtttatatca aactctccgt cctccctggc tatttgtgaa tgaaatacaa
    23761 cctgcagcag caaatcccca agttcttcaa ctatgagttc aaggtcgtcc ctttcgatag
    23821 catccagaac ctcataggtt tcttcgagga ggtacggctt taaggactct cttgtctgct
    23881 ccttatccca ggggcagcca ttctcgcctc tcaagttttc cataagctcc accaaatcct
    23941 caaagctttt gtatcttgct cccgaatcca atgcgggagg aacatagatg cttgtaagat
    24001 aatccatcca ttctatcctg tccagctcat aaagcggtat gctggcaatg cgctcctgtc
    24061 cttccactcc ggctgccctt attatatata tttcaaaatc gtccgtgtag taatccatca
    24121 gcgccagttt tacttctgaa gcaaccagct tgtcatatac ctgggttata atatttccgc
    24181 atttcttgtc gggcttctgg gttttgatgc tcaacccgtc aatgattttc aaaccgtata
    24241 tgggatctat tttcagggag cttaatataa catcaatgaa gctcaatgca ggaactatac
    24301 ttactttaat gtccttcccc gcaccggcca ggataagttc gacacttttt tccgccacca
    24361 ggggatgtcc cggcacggca tatactactt ggcccttttc gcggcacaag tccaatatgc
    24421 gccggcttat ctgcccgtat gtctcatcaa aggattcgca actgtcatat atatcatcgc
    24481 agcatgtaaa gctgatgcct gattctctta tacgttccgc aacaggatgt ttttctgtcc
    24541 tgaagaccaa tatatcagcg tttttcatta tttcgagggc ttccaggcta agctgcttat
    24601 aatcccccgg tccaagccca acaatagtta tctctttcat atatcttgtt tctccctatc
    24661 tttagtttat cttatttcag ttccggaacc ccgtatcctc aaagccccga agccctgccc
    24721 acaatctggc atctggtatc tggcgtctga atttataata accctttttt cttcaatatg
    24781 cttgaaagtc ttttgccccc cggagccatt tccagttctt ccgttgctat tccttttata
    24841 agtatcagtg caaccccata taccaatgca gcaaacgcca cgcttatcag cgttgccaca
    24901 gaattcctcc ctattgcgcc gaaaagcatc ctataactca aatatgccgc agccatcatg
    24961 gcaccggtag ctattacagg cttcacgact attttggata tcctcatact tgacttttgg
    25021 tgttttacta ttgcagaaag gttaataaag gctgtcgccg catatcctgc aacagtacct
    25081 attgctgcgc ctcttatatt tataacaggt atggaggtca aggtataact taccgcaaat
    25141 ttaaccaaag ccccaataat caggcttatt acaggtatcc tttccttccc cataccctga
    25201 agtattcctg tcatagtctg gacaagggtc aaaaatatga ctgctgtaga aagataacga
    25261 agtaccattc ccaaggttgc cggttctgcc ggatacataa gcttcattat tggatctgca
    25321 agtgtgaaaa gtccgaatgc cgcaggcagc ccaattaata aactaagccg gattgccagt
    25381 tctgacttcc tggaagcgcc atcgaagtcg cttttggcta ttgactccga tatagcagga
    25441 accaggcttg cggctaaagc tatggtaaat acctgaggca aattaataaa agcccctgcc
    25501 atgcccttaa gctgcccaag caggctggtt gcagtctcac gggaaaaacc tgctgcctga
    25561 agtctatcca taactataaa ggtatcaata acattcatta ccggcattat actaccgcca
    25621 atggtaatcg gtacggaaaa taccactatt ctctttacaa ggcttaaagc tgactctttc
    25681 tttgattgaa ggtcagccct tattccggct tttatctctc ctcttttcat tctgtatata
    25741 taaatgagag ttagaaaact gacaagccct cctatggtag tgccaaatac cgcccctcct
    25801 gctgcagatt catatcccat aggtaagaat gttatcgcca atatgaaacc gaaaacaaca
    25861 cgggcaattt gttctgtcac ctgtgataat ccactgggag tcatattctg catgccctga
    25921 aaataccctc tgaaagctcc gctcagcgct actgtgaata ttgccggagc aacacagaga
    25981 acagctacgt aggcccctgg atttttcacc atagtcgcaa agcctttagc attgaagaac
    26041 aatatcgcag atagagttat gcccaagaag gctatcagtg aaagtgcaac tttaaaggtc
    26101 ttgtgagctt catcccgcct tcctgtctct attttctccg atactaattt ggatatggct
    26161 ataggtatgg agtatgctga aattgtcagc atcattatgt aaagatcgta tgcagtctga
    26221 taatacgcca ttccctcaga gcctattatc ctgcctaaag gtattctgta tattgcgccc
    26281 agtgcctttg atattatacc tgcagctgca agaattgcag cacctttaat aaaggattgc
    26341 ttgcgatagc ccatttttga cctcccaaaa aacttacata taatataccg ataaaaagca
    26401 tattaaatag cgtaaacctt tgaagtgctt tataaatcaa tgataaatct ttttcgcaac
    26461 atatatatcc taaatgtata gcattataat tttaacatat atgcacataa ctaacaaaag
    26521 tatttatcta taattaatat agcctaaaat caagcacaat agaacaaaaa agtagcagcc
    26581 tattcgacta ctaccttgtt atatactaaa ttactgctcc atctgcttac cgaggaagct
    26641 ggctgccgtt tccgacaatt ttacctccag ttctcccatc tgtgtgtttg gatctttaga
    26701 aagtaacaaa actgcgccta tagaatcacc ctgggttatt atcggcgcta caactccggc
    26761 ggtatacttg ggggtaccct cttcatcagc agatatattg atcattttgt tttctccggt
    26821 caggcttaaa acaactgttg tcttgtcttc cattatcctt tccaatgaag ggcttaccct
    26881 cttatccagc agctctttct tcggcgctcc cgcaaccgca ataatggtat ctctgtcagc
    26941 tatgcatgtt atatgctcca tggaactgtg aagagactct acatattctt ttgcgaagtc
    27001 actgagttct cctatggggg aatatttctt aagtattact tcaccttccc tatccgtaaa
    27061 tatttccaaa gggtcccctt ctcttatacg caaagttctc cttatctctt taggtattac
    27121 aactcttcca aggtcatcta tccttcttac aattccggtt gctttcaact taaaacctcc
    27181 ttttcgaatt tacataattg tttgtaatgg ttattatttt accatctaca attttttata
    27241 cacaattttg caaattatag ataataagaa aaaaagcagt cttacgaccg catttttcaa
    27301 gtcatggtgc caggcacgtc agatagacag ttatcttcca tacttgtcta tcagttcttt
    27361 a
//
LOCUS       DAOQUR010000031        27282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2070_length_27282_cov_10.425607, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000031 DAOQUR010000000
VERSION     DAOQUR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 27282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27282
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2070_length_27282_cov_10.425607"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1073
                     /locus_tag="PKI50_07800"
     CDS             171..1073
                     /locus_tag="PKI50_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNT03639.1"
                     /translation="MIKNFNELLETAKSQKIMKIVIAAAQDEDVIKAVSQAKEIGIAE
                     PVLVGNKAKILEIMNELGIESSEFEIIDEPDLIQAARKSVKLVREGKGDFLMKGILQT
                     ADIMRAVLDKEEGLRTDNLISHVMVYEVPSYNKLLYLTDGGMNVAPDLSQKVQILENA
                     VKVCKAMKMKKIYASCIAGAETVNPKIPATVDAKAIEDMKDKWEEQGVVVQGPVALDL
                     AISSEACAHKGFKGEGGGKADILLVPYYEVGNAMGKAMTYFAGAKSAGVIMGAKVPIV
                     LVSRADSSESKLLSIALGSVVANN"
     gene            1094..2845
                     /gene="iorA"
                     /locus_tag="PKI50_07805"
     CDS             1094..2845
                     /gene="iorA"
                     /locus_tag="PKI50_07805"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNT03640.1"
                     /translation="MKQLMSGNEAIARGAYEAGVKVAAAYPGTPSTEILENIVEYKDD
                     IYCEWAPNEKVATEVAIGASIAGVRSISAMKHVGLNVAADPIFTYAYLGVNAGYVLIT
                     ADDPSMHSSQNEQDNRYYAKFAKIPMVEPSDSQESKDFMAEAFKISEQFDTPVLYRVT
                     TRVCHSKSMVEFGERIEVEPKSYERNAAKYVATPANAKIARIKVEERMKSLEEYAYSS
                     PLNKIEYNKDTKIGIITAGISYQYVKEVFGDNASYLKLGFTFPLPMKLIKEFASKFET
                     VYVVEELDPFMEEQIKAEGVKCIGKELIPPMYELNPDIVAKALLNKENETKKVDAKVV
                     GRPPVLCPGCPHRGFFYTMSKKKNTVVTSDIGCYTLGGSPPLNAVDTCICMGAGFSAG
                     MGMAKAFEASGRDNKVFGVVGDSTFFHSGITGAIDIVYNKGKMVPVVLDNRITGMTGH
                     QENPGTGKTLMGEDAVQIDPVEVLRAIGYKTVKTVDPCDLKAMDEVVTEAVNSEEPYA
                     IVTKRPCVLIKGLPPFKTKCRVDRDKCKKCKMCLKVGCPAVFFKDGTSYIDQSMCVGC
                     EVCMQVCKFDAIEKVGE"
     gene            2847..3419
                     /locus_tag="PKI50_07810"
     CDS             2847..3419
                     /locus_tag="PKI50_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNT03641.1"
                     /translation="MSDVKNILLVGVGGQGTILVSRILTAGLIKAGYDVKMSEVHGMA
                     QRGGSVTTQVRYGKKVYSPIIGKGQADILVSFEKMEAVRWIEYLKPDGVVVVNNYEIP
                     SMPIASGKEEYPRGLIEALQGSFKTVVIDAGKIAEELGNIKTQNIVLLGALVKALKMN
                     EIDWEEIIRETINPKLVDINIKAYNKGKEL"
     gene            3520..5562
                     /locus_tag="PKI50_07815"
     CDS             3520..5562
                     /locus_tag="PKI50_07815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNT03642.1"
                     /translation="MESYLQGLNKEQLTAAKHGEGPCMVLAGPGTGKTTVITSRVINL
                     IKCKSVPPENILVVTFSRAAANEMKERYGRLLGNRADEKVSFGTFHSIFYKLLRQYKG
                     YKLEDLIDENHKFNTIRNFVRRMESGFTEDEEQVRDIIGDMEYIISTMSEAEDFKPGS
                     CSSSQLKGIMKEYSEYKQKSGKYDYDDILFDCFHMLRENSGILKEVRERFKYILVDEF
                     QDINALQFEAIKLIAEPLNNIFIVGDDDQSIYGFRGAAPDILLNFERLYSGCSKIYLK
                     TNYRSTSNILTSALSVINTNNNRYSKDLEAVNGQGSLPVVFEAEDFEQEARIIASRIG
                     QMIKNGREYSDFAVIYRTNLQSRSVIDAFLDCRIPFITFDGMISIYNHWIYKDIISYL
                     KLGTGLGDTRDFIRIMNKPKRYISRDMMEKAENCGGDFIQGIIGQGSLNRLQINGVFG
                     LKNDLKRLGGMNPLNAVRYIRNVMGYDEYLKEYAASKGISTKGLSEIIEEIESSAGGF
                     KSILNYMQHTEDVVEKLGQKEYTNSGHNNVKLLTMHKAKGLEFETIFICGTVEGLTPY
                     IKDDSALRTDTEEERRLFYVAMTRAKRELYIAVPKRRYGKAVRPSRFIEEFQKSIDYS
                     SQVHAGQKVYHKIYYEGVVKEVIGKKGGTRIRVDFGGSIKELNLETCLNNEIIRLM"
     gene            5605..5856
                     /locus_tag="PKI50_07820"
     CDS             5605..5856
                     /locus_tag="PKI50_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03643.1"
                     /translation="MNYIRVIRISGSFFAREFIKPEKARKKVQYREVDEKTVAEQFLK
                     GDATVEVIFEDSDRESIKLHLESDPSLIKKYLGDRFISY"
     gene            5873..6757
                     /locus_tag="PKI50_07825"
     CDS             5873..6757
                     /locus_tag="PKI50_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT03644.1"
                     /translation="MFRFRKVSQADKSKVLEISSKIWEGHDFIQHVFDHWVSDAEGEF
                     TIGEKDGDVIGFAKYTKHSDSEAWFEGIRADEKYTGQGFGKAITQYFISRAKAEAVDT
                     IRLCTETASKECIRIVEGLGFKKDGYFTYGYKELGKDTKEPDVSKTVISIMSTATAWD
                     LITRGNSYYMSNGYISYGWVFKKATYDLTDKLVKDKCVYGVLQDGKVNSVMVLTEDSH
                     MDGGLNIGFIDGDMGSMKLLVDFAEQQAINRGMKYCEIMAPIDERIINVLEMSNFKFL
                     SEKYREVNVFVYFMKILG"
     gene            6911..7450
                     /locus_tag="PKI50_07830"
     CDS             6911..7450
                     /locus_tag="PKI50_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNT03645.1"
                     /translation="MYFTEEGKKNTEKVAELVIKAARENNIAHIVVASNTGATARHFI
                     GCGMEITCVTHAYGYGQPGVNEMNRNDREELSALGVKVLTTTHVLSGAERSLSAKFGG
                     INPVEIIANSLRMLGQGVKVCVEISVMALDAGLIPYGEPVIAVGGTGRGADTAVIIKP
                     AHAKDILNTRIQEIICKPR"
     gene            7653..8303
                     /locus_tag="PKI50_07835"
     CDS             7653..8303
                     /locus_tag="PKI50_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNT03646.1"
                     /translation="MKKVELKADNRTGSGSKQSKKLKKENCIPAILYVKGSAGRLLKI
                     DESELNNIIAKNGENVVVKLRIDGTEIPAVIKEIQRDHLQQHLIHVDFQPVTLHEIIH
                     AEVPILVVNGEKIERSGWVINKQMTELEIEGEVEKIPPAITVDAAKYKVGQVLRVADL
                     EISEELSIVNEKNEVILSILPTKDRPIDLVFNRVEPELVTTEKRDKKKEKERPDVQ"
     gene            8464..9213
                     /gene="mtnP"
                     /locus_tag="PKI50_07840"
     CDS             8464..9213
                     /gene="mtnP"
                     /locus_tag="PKI50_07840"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNT03647.1"
                     /translation="MDYKADIGVFGGSGFYSFLEDVEEIDIETPYGSTSEKISLAKTG
                     GKTVAFLPRHGKKHSFPPHMIPYRANVYAMKMLGVRHIIAPTASGSLQAGIKLGEFVI
                     CDQFVDRTWGRKDTYFEGPETKHISAAMPYDENLRNLAIKACEEAGVTVHKKGTVVVI
                     QGPRFSTKAESRWFSKMGWEAINMTQYPECILANELNIPYVNISLITDYDAGLEGDEN
                     IKPVTEEEVYRVFNENNEKVKEVIYKMIEQL"
     gene            9240..9923
                     /locus_tag="PKI50_07845"
     CDS             9240..9923
                     /locus_tag="PKI50_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNT03648.1"
                     /translation="MKGKLIIIEAGSDASGKATQAKRLYSRLRSDKYNIRKIEYPNYK
                     SNSSALVKMYLNGEFGYRPEDVNAYAASTFFSVDRYASYMKEWKGFYEDGGIVLADRY
                     TTSNMVHQASKIADEAERNSYLDWLWDLEFVKLGLPVPDLVIFLDVPPEISNNLITKR
                     NNKITGGSKKDIHESDREYLIKTYLNACRVAEKYGWKRIDCTYDNCLRSIEDIHNEIF
                     EYVIQNLPL"
     gene            10041..10586
                     /locus_tag="PKI50_07850"
     CDS             10041..10586
                     /locus_tag="PKI50_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HNT03649.1"
                     /translation="MTFVTSLETSFKKVVSAFKNPWPSMLAVLLTHIGSPLIAWFMGY
                     IFYINDPMIRFGLLIVAAVPMGVTSIIWTGIAGGDVAVSIMAVTLDTIIAPVLLPGFF
                     MAVAGKFIHIDYFQMVIQLLWMVTIPSIVGMRNNAFGMVLALSYFTPAMAISITLSVL
                     FQQPISALIAQVLSCKKTEEF"
     gene            10614..10934
                     /locus_tag="PKI50_07855"
     CDS             10614..10934
                     /locus_tag="PKI50_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03650.1"
                     /translation="MKEFSNELLQAVKMMGISARTAPKANGLDFLEIKVLNEEETVKL
                     GKAMKEYGLSSGKRNFERDGENVMNSQAGLLISINGEKHAGLNCGACGYNSCSGKNIY
                     FDRK"
     gene            complement(11031..12014)
                     /locus_tag="PKI50_07860"
     CDS             complement(11031..12014)
                     /locus_tag="PKI50_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNT03651.1"
                     /translation="MKRIIIVFTGGTIAMRIDEKTNAAVPAISGEHILQMTPGLDSIA
                     EIGFLNFSNIPGPHMTPGKMFELSKAIQYKLEVEGYDGAVVTHGTDSLEETAYLVDLT
                     TNSEKPIVFFGSMKNSSELGFDGPMNLIAATYAAVSNEAYGRGVLVVMNNEIHSASQV
                     TKTNTHTLDTFKSADFGPIGFIDNDKAYFYHDYTKRQHIGSEAIDSRVDLIKTACGMD
                     DKLLRFCVDSGTHGIVIEGMGRGNIPPTMVPGVEYALSKGIPVVLVSRCLMGKVLDSY
                     GYPGAGKELTKRGVILGDNLPGQKARIKLIVALGHTKNLAEIKDIFEKNYY"
     gene            complement(12200..13687)
                     /locus_tag="PKI50_07865"
     CDS             complement(12200..13687)
                     /locus_tag="PKI50_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT03652.1"
                     /translation="MYKLDQTKTPLFDALLEYHNNDTVPFHVPGHKRGQGMVRKFKDF
                     VGTNVLSIDVTVFHQVDSLHKPTGVIKEAQELAADAYNADYTFFSIHGTSGAIQAMIM
                     SVVTQGDKIIIPRNVHKSVTAGIILSGANPVYMHPEIDARVGLALNVTPETVRKTLEQ
                     NSDAKAVLIVNPTYYGVSTDIVEIAKIVHEYNIPLIVDEAHGPHLHFNSRLPISAMDA
                     GADICAQSTHKIVGSMTQSSLLHVRKGFVDVNRVKTVMSLLQTTSPSYVLLASLDCAR
                     MQMATEGKKLIDDAIELAGYARERINDIKGLYCFGEKVLEKKGAYGFDPTKITITSKD
                     IGLSGHDLEDILTEKYFIQPELSDLYNILCVFSFGDTKEKVDKLINALEEISAEQSCK
                     EIVKSEVLGIPNIPESVLTPRDAFNSMTIPIPLEDSVGLISAEFLMAYPPGIPILCPG
                     EIITQEIVDYVRALKAAKLYVQGTEDPEVNRIKIVSDLQIMNVNM"
     gene            complement(13878..14711)
                     /gene="speE"
                     /locus_tag="PKI50_07870"
     CDS             complement(13878..14711)
                     /gene="speE"
                     /locus_tag="PKI50_07870"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNT03653.1"
                     /translation="MDLWIREMQNENAAMTYKVKETLHTEKTKYQELAILDTYEFGRM
                     LVLDGIVQTTIRDEFAYHEMITHVPLFTHGNPKKVLVVGGGDGGTIREILKHKSVEKA
                     VLCEIDDRVVELSKKYLPEISCKLSDPRVEVFIGDGIKYVREHKNEFDVAIIDSTDPI
                     GAAEGLFSADFYKSVFECLREDGIFTAQTEAPFFSPEIVKKVFSDIESIFPITKLYMG
                     TVPTYPGVHWSFTLGSKKNDPEKTDIDSIPELDTKYYHKDLHKACFVLPKYIKNLIMQ
                     K"
     gene            complement(14745..15866)
                     /locus_tag="PKI50_07875"
     CDS             complement(14745..15866)
                     /locus_tag="PKI50_07875"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT03654.1"
                     /translation="MNVLIFSVSIGNGHDQVAHTLRNEFIRQDNSSRVRIINTINYIS
                     PLLDKVILDSYLNILKFYPKAWGKIYEKTNRLDPIIDINDITNRLMTSKLRKTTINFD
                     PDITICTHSFPAAIISKLKQKKRIGCPLVTVVTDFNIHSSYINDNTDYYVIAHEYLTY
                     IMERFGVQKEKVLPFGIPIRKEFSESYNREELIAKLGYEDKKTILVMGGGLGLGDINN
                     IVKAIDSNMEDIQIIAVAGRNNRLESKLKDLSAKNRLVVYGFVNNMHELMELSDCIIS
                     KPGGVSVSEILSREKPLVIFSPLPGQEHENVEFLLNSGAAVYTSDVRRIPALINQIFD
                     SEIRMKCFKELTGLLKKPYSAYNIVHYLTVKYGSNSKTY"
     gene            16120..16461
                     /gene="spoIIAA"
                     /locus_tag="PKI50_07880"
     CDS             16120..16461
                     /gene="spoIIAA"
                     /locus_tag="PKI50_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HNT03655.1"
                     /translation="MSIDFSYADRLLVVRINGDIDHHSCEEIRSKIDKEIASKNPRSI
                     LFDMEQVGFMDSSGIGVLIGRYKLIANGGGKAGMINVKPQIMRLCEICGLQKIIKIYS
                     SKKQAIASIKE"
     gene            16469..16900
                     /gene="spoIIAB"
                     /locus_tag="PKI50_07885"
     CDS             16469..16900
                     /gene="spoIIAB"
                     /locus_tag="PKI50_07885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HNT03656.1"
                     /translation="MKADNEMKLEFLSKSQNESFARSVVAAFVAQLDPTIEELAEIKT
                     AVSEAVTNAIIHGYSCEIGNVVIVSRIFGDTVEIEVKDFGVGIADIELARQPLYTSKP
                     ELERSGMGFTVMETFMDKVEIESLPWKGTTIRMTKKITTVR"
     gene            16910..17644
                     /gene="sigF"
                     /locus_tag="PKI50_07890"
     CDS             16910..17644
                     /gene="sigF"
                     /locus_tag="PKI50_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HNT03657.1"
                     /translation="MERVELTQDEFLELISRAQRGDSEAENFLVENNIGLVRSVVRRY
                     LNRGCEYDDLIQIGSIGLLKAIKKFDLSYNVKFSTYAIPMIIGEIKRFLRDDGIIKVS
                     RNLKEIANKAHIAKEALEKDYGREPTISEIASVLEISKEDLVVALESVQSTEYLYETI
                     HQDDGSPILLIDRISFNEGEEIEVIDRLALKEVLSALEPKERQIIMLRYFKDMTQCQV
                     AQMLGMSQVQVSRVEKKIISKLRQNM"
     gene            17847..17999
                     /locus_tag="PKI50_07895"
     CDS             17847..17999
                     /locus_tag="PKI50_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03658.1"
                     /translation="MKIFKLFLVLSVIIAVCYFSWDYYAGKLLKENRERATLVFGEQG
                     SGYYVQ"
     gene            17989..18123
                     /locus_tag="PKI50_07900"
     CDS             17989..18123
                     /locus_tag="PKI50_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03659.1"
                     /translation="MFSNRRFKQLKNILGFESETEKEIRSDIGGKIIKNSARVFEHRQ
                     "
     gene            18163..18597
                     /gene="spoVAC"
                     /locus_tag="PKI50_07905"
     CDS             18163..18597
                     /gene="spoVAC"
                     /locus_tag="PKI50_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HNT03660.1"
                     /translation="MSSKQYEQYIKDKLPKPAYLKNCIAAFIVGGFICDVAQFISNTA
                     KSYGYNQEAVSSITAASMVFLGAFLTGIGVYDRIGKFAGAGSIVPITGFANSIVSPAM
                     EFKREGFVFGVGAKMFILAGPVLVYGISSSILIGIIYYLLGK"
     gene            18629..19657
                     /gene="spoVAD"
                     /locus_tag="PKI50_07910"
     CDS             18629..19657
                     /gene="spoVAD"
                     /locus_tag="PKI50_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HNT03661.1"
                     /translation="MAGKRLGKQTLKLSSPPSVISNAAIVGPKEGEGPLSQYFDQILT
                     DDYFGEDSWEKAESKMLKEAVKSAVKASGRSNTNIDFMFAGDLLNQIVSSSFAARELQ
                     IPYIGLYGACSTMAESLCMGSLMIDGGFAEAIVCATSSHFCSAERQYRFPLEQGVQRP
                     PFAQWTVTGAGAAVIAETGSGPYITHATIGKVQDFGIKDANNMGAAMAPAAVDTITAH
                     LNETGRRPEYYDLIITGDLGNIGKAIILDMMQKEGYDITKVYNDCGSMIFDSEKQDTH
                     AGGSGCGCAAVVLNGYIAKEMKKGNINKVLLIATGALLSPTTTWQGESIPCIAHAVAI
                     EKTIGVGV"
     gene            19657..20013
                     /gene="spoVAE"
                     /locus_tag="PKI50_07915"
     CDS             19657..20013
                     /gene="spoVAE"
                     /locus_tag="PKI50_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HNT03662.1"
                     /translation="MDYLKAFLVGGAICVIGQLLMDLTKLTPARVLVAFVTAGAILQG
                     IGIYQAIVDFGGAGATTPLTGFGYSLAKGAMKEVKEAGLLGAFTGGLKSTAGGIAGAI
                     FFGYIMTLLFSPKSKK"
     gene            20025..20597
                     /locus_tag="PKI50_07920"
     CDS             20025..20597
                     /locus_tag="PKI50_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HNT03663.1"
                     /translation="MNETKKRVILVTDGDGIARKAVETAATRIGGRCISKSAGNPTPL
                     SGQKIIELVKQAPYDPVVVMVDDRGHTGTGKGEKAMLEIARSPDIEVLGVIAVASNTE
                     GVKGVSIDFSVTKNGNIVEKTVDKNGNITAGGKINGDTVDILDSIDVPVIAGIGDPGK
                     MDGKDCCDKGAPIITKAMKEIIERSRVHTH"
     gene            complement(20642..23005)
                     /locus_tag="PKI50_07925"
     CDS             complement(20642..23005)
                     /locus_tag="PKI50_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNT03664.1"
                     /translation="MSENETNSPKKKKKKKRTFSVGRFLRFIILLLLFIILVSGSIAL
                     GTAYSWVKAARPLNVDELFDLNQTTYIVDKNDKLIDKLHANENRTIATLDQIPEYLQN
                     AFIAIEDKRFREHRGIDLYRIFGALKADLASGEFSQGASTITQQLIKNVYLTPDKKWK
                     RKVVEMYYALQLERRFLKDQILEAYLNTIALGHNVAGVKEAALYYFGKELDQLTLAEC
                     ATIAGITQYPSLYSPYLNFEKSMGKKEIVLEEMLVQGLISQEEYDEAMKQEIKLSKVE
                     KEVETTYFADMIISDVTETLQKELGLSKEEAQIKLFNGGLKIIATIDTEIQNIVEETF
                     KNTKLFPESEEDANGILQPEAAVIIIENGTGEIKAVMGGRSEKVRRGLNRATQSLRQP
                     GSTIKPLAVYTPALDNGYTAGTVIDDAPVIFGNFKPRNYSNNFKGLVTAREALQNSTN
                     VVAVKILQDVGIQRSIEYLKKFGITTIVTREDNGVTNDESLSPLALGGVTNGVKPIEM
                     AAAFSVFPNKGIYIKPISFTKILDKNGNVIYENKPYKDKVISEQVAYLMVDLMRGVVR
                     GGTGGNAALSKMPVAGKTGTTTKNVDAWFTGYSPYYTASVWIGHDEPKPMNFTGGSYP
                     AKLWKAVMEEIHKNLEVKNFEKPGGLVSVSICTVSGKRPSELCALDPRGTRVRSELFI
                     KGTEPDAETICDVHVIRDACTDSKKLATEFCPAPSVQSQVFIQRIEPFIPEPESDQKQ
                     LPADLIYEAPIEPCDIHTEPTFEEDPLEGEIDLEGEIDLEGEEDEEF"
     gene            23274..23900
                     /gene="yunB"
                     /locus_tag="PKI50_07930"
     CDS             23274..23900
                     /gene="yunB"
                     /locus_tag="PKI50_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HNT03665.1"
                     /translation="MRRKFGQRGGRKKLFLSFILLIIISIYSYYIVERNIRPTILAMS
                     EINARLIATQAINEAVRSKVITDSFSKLIDYKTDNNGRISLIQANSAQMTKLAAETSI
                     EILNEIKKIGITDLKIPMSSIFGSQLFANTGPVIGVNIRPAGSVNVDFFTDFEEAGIN
                     QTRLKIYLTVKTDVQIVVPLGSNKIDVTTHIPVSETIIVSEVSEGKTA"
     gene            23996..25186
                     /locus_tag="PKI50_07935"
     CDS             23996..25186
                     /locus_tag="PKI50_07935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNT03666.1"
                     /translation="MNRLVFLVDMNAFFISCEMAMNSELKGKPAAVAGDPKKRSGIIL
                     AANYEARKYGIKTTMVLHEAKKLCPELVTVPPSHGLYEKKSKEVMDILSRYTPVIQQN
                     SIDEAWMDLTGCEKLFGSPMEIAENIMKTISEELDLWCSIGISENKFLAKMGSELKKP
                     QGITELYVKDIEKKLWPLPVREMYGIGKQTEQKLMDYAIYKIGDIANSNVEFLKKNFG
                     KYGEELYRLSRGIDNSPVVPNPIYDNKSISRSTTLPQDTNDLEYLKYILLGLAEEVGY
                     EARKSGYKGKTVSISIKYEDFKTVTRQKTVAPTYLTKDIYTNALALLETNKNKNKLIR
                     LIGLGLSNLDTDETEQLSIFELVEGEHNAHKEETIEKTIDRIRERFGDNKILRGSMLK
                     NKKG"
     gene            25281..25481
                     /locus_tag="PKI50_07940"
     CDS             25281..25481
                     /locus_tag="PKI50_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03667.1"
                     /translation="MKADKYEIKLVREESETERIEAYNKISGWLLKRYYGGCEGFGCA
                     GVFESIDGGTGRERVFDTGSEG"
     gene            25402..26919
                     /locus_tag="PKI50_07945"
     CDS             25402..26919
                     /locus_tag="PKI50_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNT03668.1"
                     /translation="MAALVYLRVSTEEQAERGYSIQVQRDEGLKKAVELGCSPESIYI
                     FNDEGVSGAFLERPQLMAALDMVKNRENNISYFICYDSSRLSRNAAHQLIIVDEIKKS
                     GAKLIFLKNNYQDNAEGRFQLTVMAAVDEYERARLRLRTEMGKRAKAGQHMLTHNPGI
                     YGYDFDQKSDTLSINEDHAKRLKQIFALLIEEHKSPAEIAGELNASGVPSPRMKQWSR
                     VTVRRMLSNPSYLGTLYIRRYDTRECYLNKFKKKGEKIKVKERPRDEWIPVKIPQIID
                     KDTWEKAQDILKESSRSRVKGFREDFILTPLLKCGMCSSIMNSKAAAKGNSLYRYYIC
                     SGKYKGVKGKNCNEALVKADDAEKAIWEQVYKSIYDFAKNEADMEKLIEGYISETGNN
                     IENILREKEKAILEKERIITMFQKGYIKEDEMCRKLKANEKSLIGLNEAAAGRNEKDA
                     SFIERLKKDCREKSFPYIIRDVLNRLDSKDKRYIIRLLISEIIISGNTVIIKGRL"
BASE COUNT         8530 a         4816 c         6480 g         7456 t
ORIGIN      
        1 agcgggacag gagaaacgtc cccgtgtccc gaaacgtcct tcgtgtcttt atttttgaaa
       61 taatacttaa aaatattagt tataggttga aggaagataa attatattgt agaataaata
      121 tggggttcta caaaattttt aagatataca aaatattttg aggtgaaata atgattaaga
      181 attttaatga attactggaa acggcaaaaa gccaaaaaat aatgaaaatt gttatagccg
      241 cagctcagga tgaagacgta atcaaggcag taagccaggc aaaagaaata ggtattgcag
      301 agccggtttt ggtaggaaat aaagcaaaaa tactcgaaat catgaatgag ttgggaattg
      361 aaagcagtga atttgagata attgatgaac cggatttgat acaagctgca aggaagtctg
      421 taaagctcgt aagagaaggc aaaggtgact tcctgatgaa gggaattctt cagactgccg
      481 atataatgag agccgttctt gacaaggagg aaggactgag gacggataat cttatcagcc
      541 atgttatggt atacgaggtt ccttcatata ataagctttt gtatttaact gacggaggaa
      601 tgaatgtggc acccgatctt tctcagaagg ttcagatact ggaaaatgca gttaaagtgt
      661 gcaaggcaat gaaaatgaaa aaaatttatg cttcatgtat tgcgggtgcg gagacagtta
      721 atcccaaaat accggcgact gttgatgcaa aagcaattga ggatatgaaa gacaaatggg
      781 aagaacaagg agttgtagtg caggggcctg ttgctttaga ccttgcaata agcagtgagg
      841 catgtgccca taaaggcttt aagggagaag gcggcggcaa agccgatata ttgctggttc
      901 cttattatga agtaggaaat gcaatgggaa aggcaatgac gtattttgca ggggcaaaat
      961 ccgcgggagt tataatgggt gccaaagtgc ctatagtact tgtatcaaga gctgactcct
     1021 cagaatcaaa gcttttatca atagcccttg gaagtgtagt agctaataat taatatataa
     1081 gggggtaaac atagtgaagc aactgatgtc aggcaacgaa gcaattgcca gaggcgcgta
     1141 tgaagccggg gttaaagtag ctgcagccta tcccggtacg ccaagcacag aaatactgga
     1201 aaacatcgta gaatacaagg atgatattta ttgtgaatgg gcacccaacg agaaggtggc
     1261 aacagaagtt gcgattggtg catctattgc gggagtgaga agcatatccg caatgaagca
     1321 tgtcggcctc aacgtagcag ctgacccgat attcacatat gcatacctgg gagtgaatgc
     1381 agggtatgta cttattaccg ctgatgaccc aagcatgcac agctcacaga atgagcagga
     1441 caacaggtat tatgcaaaat ttgccaagat tccgatggtt gagcccagcg acagccagga
     1501 atcaaaggac tttatggcgg aggcatttaa aatcagcgag cagtttgata ctcctgtact
     1561 gtacagggta acaaccaggg tatgccacag caagagtatg gtagagtttg gagagagaat
     1621 agaagttgag ccgaagagtt atgagagaaa tgcggccaag tacgtagcaa ctcccgctaa
     1681 tgcgaagatt gcccgtataa aagttgaaga aagaatgaaa tctcttgaag aatatgcata
     1741 cagctcacct ctcaacaaga ttgaatataa caaggacaca aaaataggta taataactgc
     1801 gggaatatct tatcagtatg ttaaggaagt attcggagac aatgcttcgt atttaaagct
     1861 cggttttaca ttcccgctgc caatgaagct gataaaagaa tttgcttcta aatttgagac
     1921 tgtttatgtt gttgaagaac tggacccctt tatggaagaa cagataaaag ccgaaggagt
     1981 caagtgcata ggcaaagagc ttatacctcc gatgtatgag cttaatccgg atatagtagc
     2041 gaaagccctt ttgaataagg aaaacgagac aaagaaggtt gatgccaaag tagtaggaag
     2101 gcctccggtg ctctgccccg gctgtcctca caggggtttc ttttatacaa tgtcaaagaa
     2161 aaagaataca gtagtaacaa gcgatattgg ctgttatact cttggagggt caccacctct
     2221 taatgcagta gatacctgca tatgtatggg agcaggcttt agtgccggaa tgggaatggc
     2281 taaggccttt gaggcatccg gacgggacaa caaggtgttt ggtgttgtag gtgattcaac
     2341 cttcttccat tcaggaatta cgggagcaat agacatagta tacaataaag gtaaaatggt
     2401 gcctgtagtg cttgacaaca gaattacggg aatgaccggg caccaggaga atccgggaac
     2461 aggaaagact ttaatgggtg aagatgcggt acagatagat ccggttgaag tattgagggc
     2521 aataggctat aaaacggtta aaacagtaga cccatgtgat ttaaaggcta tggatgaggt
     2581 ggtcacagaa gcagtaaaca gtgaagaacc ttatgcaata gtaaccaaga ggccttgcgt
     2641 gctaattaag ggtttaccgc ctttcaagac aaaatgcagg gtcgacaggg ataaatgcaa
     2701 gaagtgcaaa atgtgcctta aggtaggatg ccctgccgta ttcttcaaag atggcacttc
     2761 atatattgat cagtcaatgt gtgtaggctg cgaagtttgt atgcaggttt gcaaattcga
     2821 tgctatagag aaggttggtg aataatatga gtgatgtaaa gaacatatta ttggttggcg
     2881 tcggcgggca gggaacaata cttgtcagca ggatattgac cgcagggctt ataaaagccg
     2941 gttacgacgt gaaaatgtca gaggtgcacg gcatggctca gaggggcgga agtgttacta
     3001 cacaagtaag atacggcaaa aaggtttact cacctataat tggcaaaggc caggctgaca
     3061 tactcgtatc ttttgagaag atggaagctg taagatggat tgagtatctc aaaccggacg
     3121 gagttgttgt agtcaacaac tatgagattc cttcaatgcc aattgccagc ggcaaggaag
     3181 aatacccccg gggactaata gaagcactgc agggttcatt taaaacagtt gtaattgatg
     3241 cggggaaaat agcggaggaa ctgggaaata tcaagactca gaatatagtg cttttaggtg
     3301 ctttggtaaa agctctcaaa atgaatgaaa ttgactggga agaaatcata agggaaacaa
     3361 tcaatcccaa actggtagat attaatatca aggcgtacaa taaaggaaaa gagctataga
     3421 cggaaggagc tgcacatttg tgtgcagctt ttctttattt tggcattctt aaaagaaatg
     3481 atataatgta catataatat aaaggattaa ggtatactga tggaaagtta tttacaaggc
     3541 ttgaacaagg aacagctgac agctgcaaag cacggagagg ggccctgcat ggtgctggca
     3601 ggcccgggta cagggaagac aactgtaata acttcaaggg taatcaacct gataaaatgt
     3661 aagtctgtgc cccctgaaaa catacttgtc gttacctttt caagagcggc agccaatgaa
     3721 atgaaggagc ggtacggcag gcttttggga aaccgggcgg acgaaaaggt gagcttcggc
     3781 acctttcatt caatattcta caagcttcta aggcagtata agggatataa gctggaagac
     3841 ctgattgatg aaaaccataa gtttaatact atcaggaatt ttgtcagaag gatggaatca
     3901 ggctttacag aggatgagga gcaggtaagg gatatcatcg gtgatatgga gtatataata
     3961 agtactatgt ccgaagcgga agatttcaag ccgggttcat gcagcagcag ccagctaaaa
     4021 ggaataatga aggaatacag tgaatacaag caaaagtccg gtaaatatga ttatgatgat
     4081 atattatttg actgctttca tatgctgcgg gaaaactccg ggattcttaa agaagtaagg
     4141 gaaaggttca aatacatatt ggtggatgaa tttcaggata taaatgcact gcagtttgaa
     4201 gcaataaagc ttatagctga gcccctcaat aatattttca ttgtagggga tgatgatcag
     4261 tctatttatg gattcagggg tgcagctcct gatatactcc tgaattttga aaggctttat
     4321 tcgggctgca gtaaaatata tcttaaaaca aattacagaa gtacaagcaa tatattgacc
     4381 tctgccttat ctgtcataaa taccaacaat aacaggtatt ccaaagacct tgaagcggtg
     4441 aacggacaag gaagccttcc tgttgttttc gaagcggaag attttgagca ggaggcaaga
     4501 attatagcct ccagaatcgg gcaaatgatc aaaaatggca gggagtattc ggattttgcc
     4561 gtgatataca ggacaaatct ccaatccagg tcggttattg atgctttttt ggattgcagg
     4621 attcctttta ttacttttga tggtatgata tctatataca atcattggat atacaaggat
     4681 atcatcagtt atctgaagct tggaacagga ttgggggaca caagggattt tatcagaatt
     4741 atgaacaagc ctaaaaggta tataagcagg gatatgatgg aaaaggcaga aaactgcggc
     4801 ggagacttta tccagggcat aatcggccag ggcagtctga acaggctgca gataaatggg
     4861 gtattcgggc ttaagaatga cttgaaaaga ctgggaggga tgaatccatt aaacgcagta
     4921 agatatatca gaaatgtaat gggctatgat gaatacctta aggaatatgc agcttcaaag
     4981 ggaataagca caaaaggcct gtcagagata attgaagaaa ttgaaagctc agcaggcgga
     5041 ttcaaaagca tattaaatta tatgcagcat acggaagatg ttgttgagaa gctgggacaa
     5101 aaggaataca caaattccgg gcataataat gtgaagctcc tgaccatgca taaggctaag
     5161 ggacttgaat ttgaaacgat attcatatgc ggtactgttg aagggcttac accgtatata
     5221 aaggatgatt ctgccttaag aaccgatacg gaagaagaaa ggcgcctatt ctatgtggct
     5281 atgaccaggg ctaaaaggga attgtatatt gccgttccaa agcgcagata cggcaaggcg
     5341 gtgagaccgt cacgctttat tgaggaattt caaaaatcca ttgattacag cagccaggtt
     5401 catgcagggc aaaaagttta tcataagata tactatgagg gagtagtaaa agaggtgatt
     5461 ggtaaaaaag ggggcacaag aataagggtg gatttcggag gcagtattaa agagcttaat
     5521 ctggagactt gtctcaataa tgaaattata aggcttatgt agggaaatct ataaatacat
     5581 atgttttcgc ataggaggcg caggatgaat tatattaggg taataaggat ttctggaagt
     5641 ttctttgcca gggaatttat taagcctgaa aaagcgcgaa agaaggtgca atacagagaa
     5701 gtagatgaaa aaacggttgc agagcagttt cttaaaggtg atgcaacagt tgaagtaata
     5761 tttgaagatt ccgacaggga atctatcaag ctgcatttgg aaagcgaccc gtcactgatt
     5821 aagaaatatc tgggtgacag gttcatatct tactgaagag gagggagtca atgtgttcag
     5881 gttcagaaaa gtatcacagg ctgataaaag caaggtgctg gagatttcat cgaaaatttg
     5941 ggaagggcat gatttcatac aacatgtttt tgaccattgg gtcagtgatg cggaaggaga
     6001 attcacaata ggcgaaaagg atggagatgt cataggcttt gcgaaatata caaagcatag
     6061 tgattccgaa gcttggtttg agggcataag agcggatgaa aaatatacag gccaaggttt
     6121 cggaaaagct attactcagt attttataag cagggcaaag gcggaagctg tggacaccat
     6181 aagactttgc accgaaactg caagtaaaga gtgcatacgg attgttgagg gcctgggttt
     6241 caagaaggat ggatatttta catacgggta taaggagctt gggaaggata caaaagagcc
     6301 tgatgtttca aagacggtca ttagtataat gagtacggct acagcatggg acttgataac
     6361 cagaggaaac tcatactata tgtccaacgg gtatataagc tacgggtggg tttttaaaaa
     6421 agcgacttat gatttgacag acaagcttgt gaaggataaa tgcgtttacg gggtgcttca
     6481 ggatgggaag gtaaattcag taatggttct gacagaggat tcacatatgg atggaggact
     6541 caatataggt ttcatcgacg gggatatggg gtctatgaag ctgctggtgg attttgcaga
     6601 gcagcaggct ataaatcggg gaatgaaata ctgtgagata atggcaccga tagatgaaag
     6661 gattattaat gtattggaaa tgagtaattt taagttttta agcgaaaaat acagggaagt
     6721 gaatgtattc gtatatttta tgaaaatatt aggatagtct tgcggtaaat ggtttctgcc
     6781 aacaattcaa gccatagaca ttgttattga atagcagaga agcattaaac aggacaattg
     6841 attgcaaggc tattatcctg aaatacagct aaaaattcct tgcgaatact attatatgag
     6901 gagtgatact atgtatttta ccgaggaagg aaagaaaaac acggagaagg ttgcggaatt
     6961 agttataaag gctgcaaggg agaataatat tgctcacatt gtcgttgctt caaataccgg
     7021 ggctactgcc agacatttta ttggatgtgg aatggaaatt acttgtgtaa ctcatgcata
     7081 tggttatggg cagccgggtg taaatgagat gaataggaat gatagagagg aattatcggc
     7141 attaggggta aaggtactta caacaacaca tgtgctttcc ggagctgaga gatctctcag
     7201 cgcaaagttc ggaggtataa atcctgttga gataattgct aactccttaa ggatgcttgg
     7261 acaaggggta aaagtgtgcg tagagatttc agtaatggca ttagatgcag gactgatacc
     7321 ctatggtgaa cctgtaatag ctgtaggagg aaccggcagg ggtgcagata ctgcagtaat
     7381 tattaagcct gctcatgcca aggatattct gaatacaagg attcaagaga taatctgtaa
     7441 gcccagatga attgttgcgc gttgcgaatt aagcgattaa aaaaattctt cgaagcatat
     7501 cgtaagcact cgaaatccga aacctgcagc aaaaactcta ttcaacaatc ataatactga
     7561 aagaagggca gtaaagataa ttaattcgat tgtttaatta aagcaattat tgggaatact
     7621 ccatagatga agatcattta tggaggtttt ttatgaagaa ggtagagttg aaagctgata
     7681 acaggaccgg ctccggcagc aagcagtcaa aaaaattaaa gaaagagaac tgtattcctg
     7741 caatacttta cgtaaagggc tctgccggaa ggctgctcaa gattgatgag agtgagctta
     7801 ataatattat tgctaaaaac ggtgagaatg ttgtagtgaa gctcagaata gacggaaccg
     7861 aaatacctgc agttattaag gagatacaga gggaccacct gcagcagcat ttgatacatg
     7921 tggattttca gcctgttaca ttacacgaaa taatccatgc agaggtaccg atactggtgg
     7981 taaacgggga gaagattgaa agaagcggct gggtcattaa caagcagatg acagaactgg
     8041 aaatagaggg tgaggttgag aagattcctc cggcaattac ggttgatgca gcgaaataca
     8101 aagtagggca ggttcttagg gtggcagacc tggaaatctc agaggaactg tcaatagtga
     8161 atgaaaagaa tgaggttata ctctcaattc tgcctacgaa ggataggcct atcgatttgg
     8221 tgtttaacag ggttgagccg gaactggtca ctacggaaaa gcgtgataag aagaaagaaa
     8281 aagagaggcc tgatgtgcag tagaagaata atgcatcgga ggacatgggg acgtttctcc
     8341 tgtcccgctt tcagcgggac aggagaaacg tccccatgtc cttaagtttt tatacagctt
     8401 gtttatgcgg gccttttata gtaatattta attagtgtaa aaagaagaag gaggcatcag
     8461 agtatggatt ataaagctga tatcggtgtt tttggaggtt caggctttta ttcctttctg
     8521 gaggatgttg aggagattga tatagaaacc ccatatggaa gtacaagcga gaagatttct
     8581 ctggccaaaa caggcggcaa gacggttgct tttctgccaa ggcacggcaa gaagcattca
     8641 tttccgcctc atatgatacc ttacagggca aacgtatatg ccatgaagat gctgggagta
     8701 aggcacataa ttgcacctac cgcatcgggg agccttcagg caggcattaa gctgggtgaa
     8761 tttgtaatat gcgatcagtt tgttgacagg acatggggca gaaaagatac ctattttgag
     8821 ggccctgaaa ccaagcatat aagtgcggca atgccctatg acgagaacct gaggaatctg
     8881 gcaataaagg cctgtgaaga agcaggggtt accgtgcata aaaagggaac ggtcgttgta
     8941 atacaagggc caagattttc aacaaaggct gaaagcagat ggttcagtaa aatgggctgg
     9001 gaagcaatca atatgactca atatccggaa tgcatcctgg ctaatgagct gaatatacca
     9061 tatgtgaata tttcccttat aaccgattat gatgcaggcc ttgaagggga tgagaacata
     9121 aagcccgtta cagaggaaga ggtatacagg gtattcaatg aaaacaatga aaaagtaaag
     9181 gaagttatat ataaaatgat tgagcagctt taatatgcgt tatattggag agtagaaata
     9241 tgaagggaaa actcattatt atcgaggccg gcagtgatgc cagcgggaag gctactcagg
     9301 ccaaaaggct ttacagcagg ctgaggagcg ataaatataa tataaggaag atcgaatatc
     9361 ccaactataa aagcaattct tctgcattgg tgaaaatgta cctgaacgga gaattcggct
     9421 acaggccgga ggacgtaaat gcttacgccg catcaacttt tttttccgtg gaccgatatg
     9481 cttcctatat gaaggaatgg aaggggttct acgaggacgg aggtatagtg ctggcagaca
     9541 ggtatacaac ctccaatatg gtgcatcaag cttcaaagat agctgatgaa gccgagcgca
     9601 attcatatct ggattggctc tgggatctgg aatttgtaaa gctgggattg cctgttccgg
     9661 atctggtgat ttttctggat gtaccgcctg aaataagcaa taacctgata acgaaacgaa
     9721 ataataagat aacaggcggt tccaaaaagg acatacatga aagcgacagg gaatacctta
     9781 taaaaacata tctgaacgcc tgccgggtag cagagaaata cggctggaaa aggattgact
     9841 gcacttatga taattgcttg aggagcatag aagacataca caatgaaata tttgaatatg
     9901 taatacaaaa cctcccgctt taaacgggag gttcttcgat ttgccctgca tgcagtaata
     9961 atcatgcatt atcagtggct tgtgaatgat aatattaagt taataataca aagatgcttt
    10021 ccatagtatt ttttgcttat atgacatttg taacgtcact tgagaccagc ttcaaaaaag
    10081 ttgtcagtgc cttcaagaat ccgtggcctt caatgctggc cgtactgctg acacatattg
    10141 gttcacccct tatagcatgg tttatgggat atatatttta cattaatgat cctatgattc
    10201 gctttggatt gttgatagtg gcagcggtgc ctatgggtgt aacttccata atatggacag
    10261 gcattgccgg gggtgatgta gctgtgtcta ttatggctgt gactttggat accattattg
    10321 cacctgttct gcttcccggt ttttttatgg cggttgccgg aaaatttatt catatcgatt
    10381 attttcagat ggttatacag cttttatgga tggtgacgat acccagtata gttggtatgc
    10441 gtaataacgc ctttgggatg gtgcttgccc tgtcatactt tacaccggct atggctattt
    10501 cgataacact aagtgtgtta tttcagcagc ctatttctgc attaattgca caagttttat
    10561 cctgcaagaa aacagaagaa ttttaattag tttgatctga aggaggtata taaatgaaag
    10621 aatttagtaa tgagctgctt caagcagtga aaatgatggg gatttccgcc cgtactgcgc
    10681 caaaagctaa tggcctggac tttcttgaga ttaaagttct aaatgaagaa gaaactgtta
    10741 aactgggaaa agctatgaag gaatatggtt taagcagcgg caaacggaat tttgaacgtg
    10801 atggagaaaa tgtaatgaat tcacaggctg gtttgcttat atccataaac ggtgagaagc
    10861 atgcagggtt gaattgcggt gcatgcggct ataattcctg cagcggtaaa aacatttatt
    10921 ttgaccgcaa ataatattac atacctaaga ctatttatta gccggctgat aattagagag
    10981 cccaacatgt ttccacattg gcctctcgag gtaatcccat aaggtgaatt tcaataataa
    11041 ttcttctcga atatatcctt tatttcagcc agatttttcg tatggcctaa cgccacaata
    11101 agtttgatcc tggccttttg cccggggagg ttgtctccaa gaatcacgcc tctcttggta
    11161 agctctttgc ccgcccccgg atatccatag ctgtccagaa ccttgcccat aagacagcgg
    11221 gatacaagta caacaggtat tcctttggat aatgcgtatt caaccccggg aaccatagtc
    11281 ggcgggatgt ttcctctgcc cataccttca attactattc cgtgggttcc ggaatccaca
    11341 caaaaacgaa gcagcttgtc atccataccg caggcagtct taatcaggtc tacacggctg
    11401 tctattgctt ccgagccgat atgctgcctt tttgtatagt catggtagaa gtatgcttta
    11461 tcgttgtcaa taaaacctat ggggccgaaa tccgcactct tgaaggtatc cagagtatgg
    11521 gtgttggtct tggttacctg tgaggcggaa tgtatttcgt tattcattac taccagtaca
    11581 cctctgccgt aagcttcatt tgaaactgcg gcataggttg cggcgattaa gttcattggt
    11641 ccgtcaaagc ccagctccga gctgtttttc atagagccga aaaacacgat gggtttttcg
    11701 gagttggttg taaggtctac aaggtatgca gtctcttcca gggaatcggt accgtgagtt
    11761 actactgcgc cgtcatagcc ttcaacttcc agcttgtact gtattgcctt ggaaagctca
    11821 aacatttttc caggagtcat atgcgggccc ggtatattgc taaaattcag aaagccgatt
    11881 tccgctatgc tatcaagccc cggagtcatt tgaagtatat gctctccgct tattgcaggc
    11941 accgccgcat tggttttctc atcaattctc attgcaatgg tgccgcctgt aaatactatg
    12001 attatccttt tcatactctt cctccctgcc ctataaaaaa tacgtctcac gccgaatttt
    12061 tttcgctagg gaaccctatg gttcccttct acggatgcaa agcgcatcct gagcaccctc
    12121 ctttatcggc agggggcttc tcccccttgc atcccccatt gtataggaaa gttatacatt
    12181 cctatacaat agaataatat tacatattta cattcataat ttgcaggtcg ctgactatct
    12241 tgatgcggtt cacctcagga tcttccgtac cttggacata aagctttgca gctttcaaag
    12301 cccttacata atcaacaatt tcctgtgtta ttatttcccc ggggcaaagt atgggtatac
    12361 ccggaggata ggccatcagg aattcggcac tgatgagtcc cacactgtct tccaggggaa
    12421 tgggtattgt catgctgtta aatgcatccc tcggtgtcag gacactttcc ggtatattgg
    12481 gtattcccaa tacctcagac tttacaattt ccttgcagct ttgttcggcg cttatttctt
    12541 caagagcgtt tatcagtttg tctacctttt cttttgtatc cccgaaggag aatacgcaaa
    12601 ggatattgta gaggtctgac agttcaggct gtatgaaata tttttccgtc agtatgtctt
    12661 caagatcatg accggatagg cctatatcct ttgaagtaat ggtaatcttt gtagggtcaa
    12721 agccgtaggc gccttttttc tcaaggacct tttcaccaaa acagtacaag cctttaatat
    12781 catttattct ttctcttgca tatccggcca gctctattgc atcatctata agctttttac
    12841 cttcagtagc catctgcatt ctggcacaat caagggaggc cagaagcaca tatgaggggc
    12901 ttgtagtctg caggaggctc atgacggtct taactctgtt gacatcaaca aatccttttc
    12961 tcacatgcag cagtgaactt tgtgtcatgg agcccactat tttatgggta ctttgggcac
    13021 atatatctgc acctgcatcc atggctgaaa tgggaagcct gctgttgaag tgaagatgag
    13081 ggccgtgggc ttcatcaact ataagaggaa tattatattc atgaactatt ttggcaattt
    13141 caacaatatc ggttgataca ccgtaataag taggattcac aataagcact gccttggcgt
    13201 cactgttctg ttccagtgtt tttctgacag tctcgggggt aacattaaga gctaatccta
    13261 cccttgcgtc aatttcagga tgcatatata cgggattggc accgctgaga ataatacctg
    13321 ccgtaactga tttatgaacg tttcttggta ttattatttt gtctccctgt gtaacaacac
    13381 tcattatcat tgcctgtatt gcaccggagg ttccgtgtat tgaaaaaaaa gtgtaatctg
    13441 cattataagc atcggcggca agttcctggg cttccttaat aacgcccgtc ggcttatgaa
    13501 ggctgtcaac ctggtgaaaa actgttacat cgattgataa gacgtttgta cccacaaaat
    13561 ccttgaactt cctgaccatt ccctgcccgc gcttatgccc cggaacgtga aaagggacag
    13621 tatcattgtt atgatattcc agcaatgcgt caaacaaagg ggtttttgtc tggtccaact
    13681 tgtacatcgg ttacacttcc tttcggttat ctaaagtatg tccaaataat atgctaaaga
    13741 aaaagccacg aacttcgatg caataagttc aggcttctgc agagcgattt ttagttgtgt
    13801 tattcaaatg ccaaatttat tatagggcaa acctatataa aatgcaataa atattattca
    13861 aaatttgtat tttaatttta tttttgcata atcaaattct ttatatattt tggaagtacg
    13921 aaacatgctt tatgcaggtc cttatgatag tatttcgtat caagttctgg aatactgtca
    13981 atatctgtct tttcagggtc atttttctta gagcccaatg taaaactcca gtgaactccg
    14041 gggtatgttg gtacggtgcc catataaagc ttggtaatcg ggaatatact ctcaatgtcg
    14101 ctaaaaacct tttttactat ctccggtgaa aagaagggag cctctgtctg agctgtaaaa
    14161 attccgtctt ctctcaggca ttcaaatacc gacttataaa aatcggcgct aaaaagtcct
    14221 tcggcagcac caatcggatc tgttgagtca atgattgcca catcaaactc atttttatgc
    14281 tcccgcacat attttatgcc gtcgccgata aagacttcaa cccggggatc ggaaagcttg
    14341 cagcttatct caggaagata cttttttgaa agctctacta ctctgtcatc aatttcacat
    14401 aatactgctt tttcaacgga cttgtgctta agtatctccc ggattgtacc tccgtcaccg
    14461 ccgccaacta ctaaaacctt tttcggatta ccgtgagtaa aaagcggtac atgagtaatc
    14521 atctcatgat atgcaaattc atcccggatg gttgtctgga caattccatc cagtacaagc
    14581 attctgccga attcataggt atccagtatg gcaagctcct gatacttcgt cttttcggta
    14641 tgcagggttt ctttcacctt gtatgtcatt gcggcattct cattctgcat ttccctaatc
    14701 cataaatcca tttaagtctc ctccaatcta tatttattaa tattttaata ggttttgctg
    14761 ttagaaccgt actttaccgt aaggtagtga actatattat aagctgaata cggctttttc
    14821 aataatcctg tcagctcttt gaagcatttc atcctgatct cggaatcaaa tatctgatta
    14881 ataagagcag gtatccttct gacatctgag gtataaactg cagcacctga gttcaggaga
    14941 aactctacat tctcatgttc ctgccccgga aggggagaga atattaccag gggtttttcc
    15001 ctgcttaaaa tctccgatac agatactcct ccgggcttgc ttattatgca atcagacagc
    15061 tccataagct catgcatgtt gtttacgaat ccgtatacca caagcctgtt ctttgcagac
    15121 aaatccttaa gctttgattc aagcctgtta ttcctgcctg caactgcaat aatctgaatg
    15181 tcttccatat tgctgtcgat ggctttcact atattgttta tatctcccag accaaggccg
    15241 ccgcccatta caagtatggt cttcttatct tcatatccca gctttgcaat cagttcttcc
    15301 ctgttatagg attcgctgaa ctcctttctg attgggattc cgaagggaag taccttttcc
    15361 ttttgtacgc cgaatctctc cataatataa gtaaggtatt catgggctat tacatagtag
    15421 tcggtattat cgttaatata agaggaatgg atattgaagt ctgtgactac agttacaagg
    15481 gggcagccaa ttctcttttt ctgtttaagt tttgaaatta tagcagcagg aaagctgtgg
    15541 gtgcatatag taatgtccgg atcaaagttt atagtggttt tcctgagctt gctggtcatc
    15601 aatctgttgg taatatcatt tatgtctata atcgggtcaa gccggttggt tttttcataa
    15661 atctttcccc aggctttcgg ataaaacttc aatatattca ggtaactgtc aagaatgact
    15721 ttatccaata atgggctgat ataatttatt gtattgataa tccttactct tgaggagtta
    15781 tcctggcgta taaactcatt tctcagtgta tgggcaacct ggtcgtggcc attgccgatg
    15841 gaaacggaaa aaatcagtac attcattttt ttgctcctaa aacgagtaaa atattttggc
    15901 tatgggtctc atctgtatta acaattatta gtatagcaca aaaagctaat tttactagat
    15961 agatataaaa acgaaaattt ctgcgtattt gtatatatta caacaaaaac cgagcatttt
    16021 atttttggcg gtattacaaa actatacata tttctttgaa ggaaaaagct gtcatgtatg
    16081 gaatattaat atagacgaat ttgaattgga gggacatgaa tgagcataga ttttagttat
    16141 gcggacaggc tgctggttgt tcgcatcaat ggagatattg atcaccattc ttgtgaagag
    16201 ataagatcca aaatagataa ggagattgct tcaaagaacc caaggtcaat cctttttgac
    16261 atggaacagg taggttttat ggacagctcg gggataggtg tgctgatagg acggtataag
    16321 cttattgcga atggtggggg taaggcaggt atgattaatg taaagcctca aataatgaga
    16381 ttgtgcgaga tatgcgggct gcagaagatt attaagatat attccagcaa aaagcaagcc
    16441 attgcaagta taaaggagtg ataacacaat gaaagcagat aacgaaatga aacttgaatt
    16501 cctaagcaaa tcacagaatg agagcttcgc aagatctgtg gttgcagcat ttgtggcaca
    16561 actggacccg acaatagaag aactggcgga gataaagact gcagtgtccg aagcggttac
    16621 aaatgccatt atacacggtt attcatgcga aattgggaat gtggttattg tttcaaggat
    16681 attcggcgat accgtagaga tagaagtaaa ggatttcgga gtcggtatag cagatattga
    16741 gcttgccagg cagcctctgt atacatctaa gccggagctt gaaagatcgg gaatgggttt
    16801 cactgtcatg gaaaccttta tggataaagt ggaaattgaa tcgcttcctt ggaaaggaac
    16861 gaccatacgt atgaccaaaa aaataacgac agtcagatga ggtattgcta tggaacgggt
    16921 tgagcttaca caggatgagt ttttggaact tatatcccgg gcacaaagag gagactcaga
    16981 ggcagaaaat ttcctggttg aaaacaatat cggtttggta agaagcgttg tcaggagata
    17041 tttgaacaga ggctgtgaat atgacgatct tatccagata gggagcatcg gcctgttgaa
    17101 ggctatcaag aaatttgatt tgagctataa cgtaaagttc tcgacttacg caatacctat
    17161 gataattggt gaaattaaaa gatttctcag ggatgacgga ataataaagg tcagcaggaa
    17221 tcttaaagaa atagcgaaca aggctcatat cgcaaaggaa gcactggaaa aggattacgg
    17281 aagggagcct acgatatctg aaattgcatc tgtacttgag atttcaaagg aggatctggt
    17341 agtagcgctg gaatcggtac agagtactga gtatctctat gaaaccatac atcaggatga
    17401 tggttcgccg atacttctta ttgacagaat aagctttaat gaaggggaag aaatcgaagt
    17461 aatagacaga ctggcattaa aagaggtgct gtctgcattg gagcccaagg agcgccagat
    17521 tataatgctt cgctatttca aggacatgac ccagtgccag gttgctcaga tgctggggat
    17581 gtcacaggtt caggtgtcaa gggttgaaaa gaaaataatc agcaagctca ggcagaatat
    17641 gtaaatgagg acatggggac ggggacatgg ggacggggac acggggacgt ttcgcgtgtc
    17701 ccactggaag tgggacaggg gaaacgtccc catgtccccg tccccgtgtc cccgtcccca
    17761 tgtcctcatt tattttattt gtttttcgta ttaatttgcc ttaatagcca ataataattg
    17821 cataaagggt aatggagtgt tgcgcaatga agatttttaa gcttttcctt gtattatccg
    17881 tgataatagc cgtatgttat ttttcctggg attattatgc cggaaaactg cttaaggaaa
    17941 accgtgaaag ggcgactctt gtattcggag aacaggggag tgggtattat gttcagtaac
    18001 agaaggttca agcagctgaa aaatatcctg ggctttgaaa gcgagactga aaaggaaata
    18061 aggagcgata taggaggaaa aataataaaa aatagcgcaa gggtattcga acacaggcaa
    18121 tagcccattg caacaggagg catggatgta ttgaaaacaa aaatgagttc aaagcaatat
    18181 gaacagtaca taaaagacaa actgcccaag ccggcgtatt tgaagaattg cattgcagcg
    18241 tttattgtgg gcggctttat atgtgatgtg gcgcagttca tatcaaatac agccaaaagc
    18301 tatggatata accaggaagc agtttcatcg attacagctg catcaatggt gtttttggga
    18361 gcctttttaa caggaatcgg tgtatatgac cgcataggta aatttgcggg tgcgggaagc
    18421 atagtgccaa ttacaggctt tgccaattcg atagtatcac cggctatgga attcaaaaga
    18481 gagggttttg tcttcggagt aggggccaag atgttcatac ttgcagggcc ggtattggtt
    18541 tatggtattt catcttcaat tttgataggt atcatttatt atttgctcgg gaaatgagaa
    18601 ggaaaattat tattttggaa gtgataggat ggcaggaaaa aggctgggga aacagacact
    18661 gaagctttct tcaccccctt ctgtgatatc caatgccgca atagtagggc ctaaggaggg
    18721 tgagggccct ctttcacagt actttgacca aatattgacg gatgactatt ttggagagga
    18781 tagttgggaa aaggctgaga gcaagatgct caaggaagca gtaaaaagcg ctgttaaagc
    18841 ctccggaagg agcaatacaa atattgactt tatgtttgca ggtgatttgc ttaatcagat
    18901 agtatcttcc agttttgcgg caagggaatt gcagattccg tatatagggc tttacggagc
    18961 atgctcaact atggctgaat ccttatgtat gggctcactg atgattgacg gaggttttgc
    19021 agaagcgata gtatgtgcta catcaagtca tttctgcagt gccgaaaggc agtacaggtt
    19081 tcccttggag cagggagtcc aaaggcctcc tttcgcacaa tggaccgtaa cgggagcagg
    19141 ggctgcggta attgcggaga caggctccgg accgtatatt actcatgcta ccattggaaa
    19201 agtacaagac tttggaataa aagatgcaaa caacatgggt gctgcaatgg ctccggctgc
    19261 tgttgataca attacagccc atttgaatga aacgggcagg aggcctgagt actacgacct
    19321 gataataaca ggagacttgg gaaacatagg aaaggctata atattggata tgatgcaaaa
    19381 ggaaggttat gatattacta aggtttataa tgattgcggc tcaatgattt ttgacagcga
    19441 aaaacaggat actcatgcag gaggaagcgg ctgcggatgt gctgccgttg tattgaacgg
    19501 ttatattgca aaagaaatga agaagggtaa tattaataaa gtgcttttga ttgcaaccgg
    19561 agcattgctt tcaccaacca ccacctggca aggggaatca atcccgtgta tagctcatgc
    19621 tgttgccata gaaaaaacaa taggagttgg tgtgtgatgg attacctgaa ggcatttttg
    19681 gttggaggag caatctgcgt tatcggacag ctattgatgg atcttactaa actgacacct
    19741 gccagggttc ttgttgcctt tgtaactgca ggcgcaattc ttcaagggat aggaatatat
    19801 caagcaatag tggattttgg aggagcaggt gcaacaacac cgctgacagg gtttgggtat
    19861 tctctggcaa agggtgcaat gaaggaggta aaggaggcag ggttgctggg cgcctttaca
    19921 gggggcttga aaagtactgc aggaggtata gcgggagcta tttttttcgg gtatataatg
    19981 acattgcttt ttagtcccaa atcaaaaaaa taggagacag ttgtatgaat gaaacaaaga
    20041 aaagagtcat actggttacc gacggcgatg ggattgcaag aaaagcagtg gagacagccg
    20101 caacgaggat aggagggaga tgcatttcaa agtcagcggg caatccgacc ccattgagcg
    20161 gtcagaaaat aatagagctg gttaagcagg cgccatatga tccggtagtt gttatggttg
    20221 acgacagagg gcataccgga acgggaaaag gggaaaaagc catgctggaa atagcgagga
    20281 gccccgacat cgaagtactg ggagtgattg cggtggcatc caataccgaa ggagtaaaag
    20341 gagtcagtat tgacttttcg gtgacaaaga acggaaatat tgtggaaaaa acagtggaca
    20401 agaatggaaa tataaccgca gggggaaaaa taaacgggga tacggttgat atacttgatt
    20461 ctattgatgt tcctgtgatt gcaggtatcg gtgatccggg caagatggac gggaaggact
    20521 gctgcgacaa aggggcgcca ataattacaa aggcaatgaa ggaaataatt gaaaggagca
    20581 gagtgcatac acactaactg ctcctgttgc tttaattcgg ctccggtatt atttcattat
    20641 gctaaaactc ttcatcttct tcgccctcca ggtctatctc gccctccagg tctatctcgc
    20701 cctccagcgg gtcctcctca aaagtcggct cggtatgaat atcgcagggt tctatcggtg
    20761 cctcatatat caaatctgcc ggaagctgtt tctgatccga ttccggttcc gggataaaag
    20821 gctctattct ttgaataaat acctgggact gtacactggg tgccgggcag aattctgttg
    20881 caagtttttt tgaatccgta caggcatctc tgattacatg aacatcacaa atagtttccg
    20941 catccggttc ggtgccttta ataaacagct cggatcttac cctggtgcct cttgggtcaa
    21001 gggcgcacag ttcagagggc ctttttccgg ataccgtaca gatgcttaca gataccaatc
    21061 ccccgggctt ctcgaaattc ttgacctcaa ggtttttatg gatttcttcc ataacagcct
    21121 tccaaagctt tgcaggataa ctgcctccgg tgaagttcat aggcttgggt tcatcatgcc
    21181 ctatccatac agaggctgta taatacgggc tgtagccggt aaaccatgca tctacatttt
    21241 ttgtggtagt acctgttttt ccggcaacag gcatcttgga taatgctgca ttaccgccgg
    21301 tgcctcccct tacaacgccc cgcatgagat ctaccatgag ataggcaacc tgctcgctga
    21361 taaccttatc cttatatggt ttgttttcat aaatcacatt cccattctta tcaagaattt
    21421 ttgtaaagga gatgggtttt atataaatgc ccttattggg gaatacactg aaagcagcgg
    21481 ccatttctat aggcttgact ccgttggtca ctccgccaag ggcaagaggg gaaaggcttt
    21541 cgtcattggt gacgccgtta tcctctctgg tgactatagt tgtaattccg aattttttca
    21601 gatattctat ggaacgctgt attccaacat cctgcaatat tttgaccgca accacatttg
    21661 ttgaattctg caaggcttcc cttgcagtta caaggccttt aaaattattg ctgtagttac
    21721 ggggtttgaa gttcccaaaa ataacaggag cgtcgtctat aacagttccg gcggtatagc
    21781 cgttatccaa agccggtgta taaacagcaa gaggctttat ggtagaaccc ggctgccgta
    21841 aggattgagt agccctgttc aggcctcttc ttaccttctc gcttctgcct cccataactg
    21901 ccttgatttc gccggttccg ttttcaatta ttattacggc tgcttcaggc tggagaatgc
    21961 cgttggcatc ttcctcactc tcagggaaga gcttggtatt cttgaaggtt tcttccacta
    22021 tgttttgaat ttccgtatct atagtggcta ttatcttaag tcctccgtta aaaagcttta
    22081 tttgagcttc ttccttgctt agtcccagtt ctttctgcag cgtttccgtg acatcgctga
    22141 taatcatgtc tgcaaaatat gttgtttcta cttccttttc tacttttgac agtttgattt
    22201 cctgcttcat agcctcatca tactcttctt gagaaataag gccttgtaca agcatttctt
    22261 caagcacaat ttcttttttg cccatggatt tttcaaaatt caaatagggc gagtataagg
    22321 aaggatactg ggtaataccc gctattgttg cacattctgc caaggtcagt tgatcaagct
    22381 cttttccaaa ataataaaga gctgcttctt taactccggc aacattatgc cccagtgcga
    22441 ttgtatttaa gtatgcttcc aatatttgat ccttaagaaa tcttctttca agctgaagcg
    22501 cataatacat ttcaactact tttcttttcc atttcttgtc cggagtaagg tagacattct
    22561 tgataagctg ctgcgtaatt gtacttgcac cctgggaaaa ctctccgctg gcaaggtcgg
    22621 cctttaaggc gccaaagatg cggtacaggt caattcctct gtgctctctg aatcgtttgt
    22681 cttcaatagc tataaatgca ttctgcaagt attcagggat ttggtccaat gtggctatgg
    22741 tgcgattctc attggcgtgc agcttgtcaa tgagcttatc attcttatca acaatatatg
    22801 tagtctgatt caagtcaaac aattcatcca cattaagcgg ccttgcagct tttacccagg
    22861 aatatgcagt acccagcgca atgctgccgc tcacaagaat tataaagaga agcagaagta
    22921 tgataaatct taagaacctg ccaacagaga aggttctttt tttcttcttt tttttcttgg
    22981 gagagtttgt ctcgttttcg ctcattttat acctccgcaa gtataaatgc tgtatgtaat
    23041 gaaattatta tatcatagaa gattttttct ggcaaaccca gtgaagttca acgctgctgc
    23101 catataagcc gcctttttga ttcttgacat ttttgctctc ttttaatcct gaatatatta
    23161 ttttgacgta aaatatgcat ttttgtcaca gcatgtgaat gtattactaa atataaatat
    23221 ttgtaatatg gataactttg aatcatatat taataaatag ggggtgtgtg ctcatgagga
    23281 ggaagttcgg tcaaaggggc ggcagaaaga agcttttctt aagctttata ctgctgatta
    23341 tcatttcaat atattcatat tatattgttg aaagaaatat caggccaaca atacttgcaa
    23401 tgtcggagat aaacgcaaga cttattgcaa cacaggcaat aaatgaagca gtaagaagca
    23461 aggtaatcac cgattcattc agtaagctta tagactacaa gaccgacaac aatggaagga
    23521 tatccctgat acaggcaaac tcggcacaaa tgaccaagct ggcggcagaa acctcaatag
    23581 agattttaaa tgaaataaaa aagatcggaa tcacagatct taaaattccc atgtcaagta
    23641 ttttcggcag ccagctattt gccaatacag gccctgtaat aggtgttaac attcgacctg
    23701 cgggcagtgt aaatgtagat ttttttacgg actttgaaga ggcgggaata aatcagacaa
    23761 ggcttaaaat atacctgacg gtcaagacgg atgttcagat agtcgttccc ctgggaagca
    23821 ataaaattga tgtaaccaca catataccgg tttctgaaac aataattgta agcgaagtat
    23881 cggaaggaaa aactgcataa gctcttgaac aaataaaagg tacatttgag ccatatagat
    23941 tggtataatg gaaagagaac tgttgtgcgg gattcgctgc ggaggtgaac aaaggatgaa
    24001 caggctggtt ttcctggttg atatgaacgc attttttata agctgcgaaa tggcaatgaa
    24061 ttccgagctg aagggaaaac ctgcggctgt tgcgggagac cccaaaaagc gttcgggaat
    24121 aatacttgcc gctaattatg aagccaggaa atacggtatc aagacaacaa tggtgcttca
    24181 tgaagcaaag aagctgtgcc ccgagcttgt aacggtgccc ccgagccatg ggctgtatga
    24241 aaagaaatcc aaggaggtta tggatatact gtcaaggtat acacctgtaa tacaacaaaa
    24301 cagcatagat gaggcatgga tggacctgac gggatgtgaa aaactctttg gaagtcctat
    24361 ggagattgcg gaaaacataa tgaagactat ttcagaagag ctggatctct ggtgttcaat
    24421 aggaatttcg gaaaacaaat tccttgcaaa gatgggatcc gaactgaaaa aacctcaggg
    24481 aattacggag ctttatgtaa aagacataga aaaaaagctg tggcctctgc ctgtaagaga
    24541 aatgtacggt ataggaaagc agacggagca aaagctgatg gactatgcaa tatataaaat
    24601 aggtgatatt gcaaacagca atgtggaatt tctgaaaaaa aactttggga aatacggcga
    24661 ggaactatac aggctttcaa gggggataga caattctccg gtagtaccca atccaattta
    24721 tgataataag tccatcagcc ggtctaccac attgccgcag gataccaacg accttgaata
    24781 tttaaagtac attttgctgg gtctggctga agaggtaggc tatgaagcca gaaaatcagg
    24841 gtataaaggg aaaacagttt caatttctat aaaatatgaa gactttaaga ctgttacaag
    24901 gcagaaaacg gtggcaccta cttatctgac gaaagacatt tataccaatg cactggcact
    24961 gcttgaaacg aataagaaca aaaacaagct tattcggctt atagggcttg gcttaagcaa
    25021 tcttgacacg gatgaaacgg agcagctttc gatatttgag ctggttgaag gagagcataa
    25081 tgcacataag gaagagacca ttgagaaaac aattgacaga atacgggaac gttttggaga
    25141 taacaaaata ttaaggggct cgatgttaaa gaacaaaaag ggttgatgat ataccaaagc
    25201 taaaggaagg acggaaatcc ttcctttaat aatgtgcagg tacaagtctt atcaaaatat
    25261 ttgatattgc aggtggtatt gtgaaagcag ataaatatga aatcaaactt gtaagggaag
    25321 aatcggagac agagagaatt gaggcttata ataagatatc tggatggtta ttgaaaagat
    25381 attatggagg ttgtgaaggg tttggctgcg ctggtgtatt tgagagtatc gacggaggaa
    25441 caggccgaga gagggtattc gatacaggtt cagagggatg aaggtttgaa aaaggctgtt
    25501 gaactgggct gcagtccgga aagcatatat attttcaatg acgagggtgt ttcgggagct
    25561 tttctggaaa ggccgcagct aatggccgct ctcgacatgg taaaaaatag agaaaataat
    25621 ataagttatt ttatatgcta tgattccagc agactgtcaa ggaatgccgc ccatcagtta
    25681 ataatagtgg atgagataaa aaaaagcggc gcaaagctca tttttttaaa gaataattat
    25741 caggataatg cagagggccg ttttcagtta acggtaatgg ctgcagtgga tgagtatgaa
    25801 agagcaagac tgaggctaag aacggaaatg ggcaagaggg caaaagcggg acaacatatg
    25861 cttacccaca atccgggaat atacggttat gattttgatc aaaaatcaga tacattaagc
    25921 ataaatgagg atcatgcaaa aagattgaag caaatttttg cattgcttat cgaagaacat
    25981 aagagcccgg cggaaatagc gggagaattg aatgcttcag gtgttccaag ccccagaatg
    26041 aagcagtgga gcagggttac agtgagaaga atgctttcga atccctcata tctgggcaca
    26101 ctatatataa gaagatatga tacaagagaa tgctatctta acaaattcaa aaaaaaaggc
    26161 gaaaaaataa aagtgaaaga aaggccgcgg gatgaatgga taccggttaa aatacctcag
    26221 ataattgata aggatacctg ggaaaaagca caggatatat taaaagaatc aagccgatca
    26281 agggtcaaag gatttcggga ggattttata ttaactcctc tgctgaagtg tggaatgtgc
    26341 agcagtataa tgaatagcaa agctgcagca aaaggtaatt ccttataccg ttattatatt
    26401 tgctccggaa agtataaggg agtaaagggg aaaaactgta atgaggccct tgtcaaagcc
    26461 gatgatgctg aaaaagcaat atgggagcag gtatataaaa gcatttatga ttttgcaaaa
    26521 aatgaggcgg atatggagaa gttaattgaa ggatacatat ctgaaacagg aaataatatt
    26581 gagaatatat taagagaaaa agaaaaagct atattagaaa aggaaagaat tattacaatg
    26641 tttcagaaag gatatattaa ggaagatgaa atgtgcagaa agcttaaggc taatgaaaag
    26701 tcactgatag ggcttaacga ggctgcagca ggaagaaatg aaaaagatgc gtctttcatt
    26761 gaaaggctta aaaaggactg ccgagaaaaa agctttcctt atattatacg ggatgttttg
    26821 aacagattgg acagtaaaga taaaaggtat ataatcaggc tgttaatctc agaaataatt
    26881 atttcgggca atacagttat tattaaagga aggctctgat gttttctatt attgatacta
    26941 cgcttgtagg cgatgttccg gatagctatg taataacacc aaaacagaat atacctgact
    27001 atgctcatta agaatgtata aatgtaagat ttttattgaa aagcacattg ttggatactg
    27061 taaaacatag cgaatgcaag agatatatat agaaataaca agtaatttag tgatagaata
    27121 aaaaaataat aaaattcggc gaaaaggcga attgagcaca cctcttattt atgatattat
    27181 tattaagcgt tgacggcaaa acaagctgtt gatgcttaaa aattctctga tccttgaaaa
    27241 ctaaacagtg taaaatgata accaaatgaa agtttgaagt gc
//
LOCUS       DAOQUR010000032        24344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2421_length_24344_cov_10.883486, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000032 DAOQUR010000000
VERSION     DAOQUR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24344
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2421_length_24344_cov_10.883486"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2569
                     /locus_tag="PKI50_07950"
     CDS             <1..2569
                     /locus_tag="PKI50_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03669.1"
                     /translation="GLESGSTTVTVTASKEGYLPAVKSFTVTVVTDYEEEGDWDKSII
                     GDYGEYTDIALDSKGMPHIIFEASSVEYRHWNGSSWQEEEEPQIGSSQGSLAIVKIDG
                     NEYSLISYNLNGCFGYDVFDGEYWSHQSFGDEGVTVAASSIDTIMYNGEYGTGVSYYD
                     EENGDLYFRFDTDPIDIILDSWGDPFPVDGENSDAGKSSSLVFDEDGLAYIAYYDYSS
                     GGEDGSLKLAVINDLLDPELDIMEVDDQVGYDDGQYVSVSQAVYNESTAIHLAYYDAV
                     NKNLKYAMWLDEEWFIETVDDSEGDVGKYASLVLDYDGNPHISYYAESDSGGALKYAR
                     RDPEHEGEWLIEVIDQGEEVGLYSSIAFDNYFSIPHFAYTAMTGGDRVVKHATRQGIM
                     NTIEPGVLSGTAETFEDADFLIESFENEICYVWREVWGEIDYLGDEDYIISEDGDVLT
                     ITLKSDYLNSLEYGIHKIWFYFDYGLPVLLTIDIEENLEAYTLRSNPDLAYDPMNNRY
                     LMVYEKIIGDTNPGIWGRFISADGEQDTEFPISAEIYCNEPKVVYNPVNNNFLVTWSH
                     YPNYEEPLIYAQILDDEGEPYGDFGNFAVCPETTGMGYGQGRPAVAVNTSNGDYLIAW
                     EEEAGGDLQDDIYGQLLDQDGVQIGEKQTLVSMDWYQSDSVLAYSAAGNKYLLAFVDS
                     NDILGWYIGADGVPEGAIQEIALDQNRPDEEQFDPDVVADSDGRILVVWGDNRNEDWI
                     SNIYGRFFNTDGNMEDEFIVYQSEVDQYEPSAAINTADGSCLVAWRDAYDRLYGQKFT
                     ADGTGIGEPVEIDSDGEGFGNAAIVYNPEENNYLAVYEDYNSYPHKIKYKILYPQQP"
     gene            2621..3448
                     /locus_tag="PKI50_07955"
     CDS             2621..3448
                     /locus_tag="PKI50_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT03670.1"
                     /translation="MTLSDFTITFWGVRGSRPVPGPRTVIYGGNTPCVEVRIGKRLII
                     LDAGTGITNLGSKLMQEPGPVIGDIFITHVHWDHIQGFPFFQPAFRIGNSFCLYGERK
                     GNVSFAASIKRTMRHPHFPVPLEHMGAALHFTEIEPGDMLELGDGITVRTAANNHPDG
                     GISYRIQYGNKSCCYVTDYEHGPEKAQALLELCAGTDLLIYDACFTAAEYPGYQGWGH
                     STWEEGLALLEATGAGRLILFHHKPERSDEEMAVLETNITGQYPMVKAAREGMVISL"
     gene            3445..4932
                     /locus_tag="PKI50_07960"
     CDS             3445..4932
                     /locus_tag="PKI50_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT03671.1"
                     /translation="MSIANAKLGDHLEELLNIGIALSAEKNHNKLLERILKEARRITN
                     ADAGTLYLCEQDHLVFKIIHNDTLGIYQGGQGEPVDLPPVPLDPANVSSWAALHKKSV
                     NIPDVYAADNFDFSGPKKYDAITGYRTGSMLVVPLENHEGEVNGVLQLLNAKNEKSQI
                     IPFAPELEKIVFSLASQAAVSLTNMQYIQEIDNLFHSFVQVMATAIDARTPYNVNHTR
                     NIARLAEKFAAYIDSLAGGPFSSEKFPESRTAQLVMAAWLHDIGKVAIPLNVMDKPSR
                     LGERIEYVRQRLELVENRLIRSFLEKKAAGAAGREQEEGHRQQLDRLSQARELIEKAN
                     HPATFVDQDLVRELSELAQWEPLPGVRLLMDEEFKCLSIPKGTLTAEERKVMESHVAV
                     AQRMLENIRFNSRYKDVPAWALSHHELLDGTGYPQGLKAGEIPLEARILAVLDVYDAL
                     TASDRPYKKAMPSNAALNILEKMAEEGKLDNLLVAAFKESRIWEE"
     gene            4947..6725
                     /locus_tag="PKI50_07965"
     CDS             4947..6725
                     /locus_tag="PKI50_07965"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT03672.1"
                     /translation="MKNNIFNKKYILIFLLCMILGFSAYVDIFTTLDSKMQDSFLRKS
                     GPVDTEIVLVAIDDQSLEDLGRFPWPRYVHAELLRILSQAQSAAIGIDIIFSEAADDP
                     EEDLALAEAIREAGNVVLPVYAGLEGFSARQGAIKTDNLVVPFPDFPDLFITGHINTF
                     PDPDGIIRKTALYLDYQGQTVPSFAWRIYEQYCKSKGLPAPDLNRLPLDPIKRLEINY
                     AGIPGDYEHVSYSQVISGEVPPEFFRGRIVLVGSYTVGIGDFYFTPLAPEVPMYGMEI
                     HANIIQNLLRGSYLQRAPFSASLLAIVIFGAAAAFGFTRLNPQKGVLLLAVLAACYLA
                     VARLMYDKGYLLSLLYPLLLLALGYLVALAANYISELLERRRITGVFSRYVVPQVVDK
                     LLEGGEKALQLGGSRREITVLFVDIRGFTPLSEAAAPEEVVEILNEYLTLVAQCIFKY
                     GGTLDKFIGDAAMAMFNAPLDLEDHCLKAVQAAWAMKEGATPLQQRLEEKFGRTVQFG
                     IGVNTGPAVIGNIGASFRMDYTAIGDTVNTAARLESNAKPGQILISQDVYEKVKERVA
                     AANLGEIKVKGKAQGVNVFQVDGVHQ"
     gene            6867..7259
                     /locus_tag="PKI50_07970"
     CDS             6867..7259
                     /locus_tag="PKI50_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT03673.1"
                     /translation="MTHFKQLSEVLRNYYDIPDQELDKINSLFYPCKLKKGEYFVRSG
                     YMADSIGFIVTGLLRYFYTDKNGVEYTKAFAAENDFAVSYASMLLKKPSAFQLRHWKI
                     PNCFASIIQTGRSCWTMYHVGILSLERL"
     gene            7745..7966
                     /locus_tag="PKI50_07975"
     CDS             7745..7966
                     /locus_tag="PKI50_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03674.1"
                     /translation="MRGFHTVQSDSTVYGYYAWNSLILVGKAEGNKLSGHIYDGIFEI
                     RMNPDGKSYSGFIFDNSAAEAGYWTGIRK"
     gene            8102..8920
                     /gene="fdhD"
                     /locus_tag="PKI50_07980"
     CDS             8102..8920
                     /gene="fdhD"
                     /locus_tag="PKI50_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNT03675.1"
                     /translation="MECSYNSNAVEIEVIKVNGSESKIKNEQVVTERALTVYLNGQEF
                     VTMVYSPGHEKELVTGFLALEGIIKRPDQLVKFSYDMQEGLAWADTAEKSILGETLFL
                     KRYMTSCCGKGRTSFYFANDARLAQKIDNPLQITSKDVSYYINLMEEKSELFRRTGGV
                     HGGALASDGQLQYFAFDIGRHNVLDKLYGQAFLEGIDISRKIIVFSGRISSEILIKTA
                     KMGCPILIGVSAPTDLALDLAKELGITVIGFARQERMNLYTHPERIIISEERSF"
     gene            9086..9178
                     /locus_tag="PKI50_07985"
                     /pseudo
     CDS             9086..9178
                     /locus_tag="PKI50_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
     gene            complement(9188..10018)
                     /locus_tag="PKI50_07990"
     CDS             complement(9188..10018)
                     /locus_tag="PKI50_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT03676.1"
                     /translation="MKKSVFKTEAGRDKVRAYYNRVLDQFPFGQRYVETTFGRTFMLT
                     AGHESNPPIILLHGSCSNSAFWFPEIMALSNYFRVYAVDIIGEAGNSEEYRPDLCSNA
                     FALWMKDVLDTLGLERAALIGNSLGGWMALKFATAYPERVSKLILIASGGLAQIRPQF
                     LINADQTRQTNGTVPVSPSIMGEQSIPKEVLDFMNLIVENYNPIRELPVFADEQLLRL
                     NMPILFIDGENDAIIDAEKSAQRLSRLIPSAVIHLLPNCGHVVANSAEYIIPFLMKTH
                     "
     gene            complement(10024..10464)
                     /locus_tag="PKI50_07995"
     CDS             complement(10024..10464)
                     /locus_tag="PKI50_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT03677.1"
                     /translation="MKGYYTVQQISDMLDIHPKTIQRYIREGKLRASKVGKGWRITGH
                     DLSTFVESNGDDGSCSGNRPVHCISASSVIDITAGGKEDAIRIMNTLTAALNSKPPEY
                     GQSSMQSQYIEGENMVRVTLWGDIRFMAVMMDTIASLTEHNEKE"
     gene            10653..13202
                     /locus_tag="PKI50_08000"
     CDS             10653..13202
                     /locus_tag="PKI50_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HNT03678.1"
                     /translation="MEKLVIGILAHVDAGKTTLSESMLYVSGKIGKPGRVDNKDAYLD
                     TYELERARGITIFSKQAVLEIGETQIILMDTPGHADFSAEMERTLQVLDYAVLVISGA
                     DGVQGHTKTLWRLLDIYQIPVFIFVNKMDQKGTDKDKLMEGMKKQLNDGCIEFGYITA
                     AEFYEQLAMCDDIIMEAYLKTGHIEAEQIRKAVRRRKVFPCFFGSALKLEGIGQFMQG
                     IAKYATAPCYPDEFGAKIFKITRDEQGNRLTHLKITGGKLKVKDVLAGGIRKEKVNQI
                     RIYSGQKYEAVSEAEAGSVCAVTGLSRGKPGEGLGIEAAAPAPVLEPVLIYRILLPEG
                     SDPRAMMPRLRQIEEEEPELNIYWDEQMQEIKVRIMGEVQLEILQSLIQSRFGVPVSF
                     DAGGIVYKETIADVVEGVGHFEPLRHYAEVHLLLEPGEPGSGLQFGTECSEDILGINW
                     QRIILTHLQEKEHIGVLTGSVLTDVKITLVSGRAHNKHTESGDFREAANRAVRQGLRS
                     AESVLLEPYYAFQLELPEKMIGRAMSDIEKMHGTGELLQTDGETAVLAGSAPVIEMRN
                     YQKDVTAYTKGMGRLFCSLKGYGPCHNTEEVIASIGYDPERDAENPAGSVFCTHGAGF
                     SVAWDEVKNYMHVESYIKKRDDFSGEAATEKAASEEGRISLEEIDQIINKTYYGNQGK
                     KSAWKRLKTTRENYCGPVDRVRREKEPKEEYLLVDGYNIIHAWPELKELAEVDIGSAR
                     MSLLDSLSKYQSVRQSNVIVVFDAYRVQGHREEMTDYHNIRLVYTKEAQTADQYIEKF
                     ARDNRDKYDITVATSDGLQQIIVRGAGCSLLSARELKIEIERKEKSYGQDN"
     gene            13165..14085
                     /locus_tag="PKI50_08005"
     CDS             13165..14085
                     /locus_tag="PKI50_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03679.1"
                     /translation="MKERKSPMDKIIEILENQGPLTGKELLEKTGMDEFTAWKNCNSS
                     ERIVTKTIGTRYLRLDRLVAGYARLSPSIIREFFGYTVIGTEKNLPDILRKAEYLSQE
                     IIRISKNKFELAKETITKIVESQAASNIIKGGACFIIAGDVAYEMSHLEPRPEVSTGE
                     LVKGSDLDIVAVTQGLPGNIINALDTSIYEHKYYLLKNPSYNEEIDYIVKDISKVKEQ
                     LKFDSFESMVASKILYEGKFLYGNFDIFKQIKKMLIDEGIPEKIRVLEEKALLNRNNA
                     KTQLLKYEGKLTDEESMKLFYTKEEKEEFF"
     gene            complement(14095..14910)
                     /gene="nagB"
                     /locus_tag="PKI50_08010"
     CDS             complement(14095..14910)
                     /gene="nagB"
                     /locus_tag="PKI50_08010"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNT03680.1"
                     /translation="MDIIVRENYDELSKYSAKLIARLVRSKPDCVLGLATGKTPIGTY
                     KELIRMHKEEGLDFSQVRTFNLDEYYGIGIDLDKPYESDQSYARFMHEELFKHINNKK
                     ENIHIPDGLTKKPEEFCKWYEEEIKKAGGIDLQLLGIGGDGHWAFNEPGSSLASRTRI
                     QALTQQTLDDNYESFYKKAGINRAHMPHFAITMGIGTILEAKNIIMLANGIKKADVVA
                     KCIEGPVTSQITASAIQLHSGGITVVLDKDSSSKLKGLHHYMHVEKLKQEYGF"
     gene            15093..15395
                     /locus_tag="PKI50_08015"
     CDS             15093..15395
                     /locus_tag="PKI50_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HNT03681.1"
                     /translation="MSNRTIFDEIRKNLFPRLEQYVPVVARVHGGNHPEFHEVRRLFD
                     EIIKKTKKAAPEKPELSEEFARLREITNNYTVPEDVCESYEAVYNMLAEADKAYQA"
     gene            15441..17294
                     /locus_tag="PKI50_08020"
     CDS             15441..17294
                     /locus_tag="PKI50_08020"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNT03682.1"
                     /translation="MQRFILSKKNHIALISAILITIGFTGELGFKNEAVAVWSLTAAS
                     VLGAAPIAIQAYQALRVKVVSIDVLVTIAVIGAFLIKNLEESAVVTFLFLFGSFLEQR
                     TLNKTRSAIKELTEMAPESALKQMESGEFEEVDVDEVDVGDILLVKTGAKVPVDGTVL
                     TGEGHVNEASITGEAVPAGKEKGSQVYAGTILENGTIQIVADRVGEDTTFGRIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLSSIVWLISRDIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLLKGSEVIHDFSKVDTIVFDKTGTLTIGNPKVADKEIYADNAD
                     EVLGYLASVESESGHPLAKAVAEYIGDTKAYTVEKTDVVKGGGIVAQAEGHRVAVGNA
                     ALMEQEGVHLDEKARADIARFEKNGNSLVLTSVDGELKALMGIRDQIRPGVKEDLQKL
                     KKLGVKNLVLLSGDNQGTVDLAARELGLTEAHGHMLPEDKSTYIKELQSKGQIVAFVG
                     DGVNDSPSIALAQIGIAMGSGTDAAIETSDIVLMNSDFSRLPHALGLTKATANNMRQN
                     IVIAVGVVLVLLASVFFSEWMNMSIGMLVHEASILAVILNGMRLLSYRLRY"
     gene            17334..17564
                     /locus_tag="PKI50_08025"
     CDS             17334..17564
                     /locus_tag="PKI50_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNT03683.1"
                     /translation="MKKAAIQLETLTCPSCMLKIENAVKSLEGVERESVSVSFNSSKV
                     KLDFDDEKISIKDIENAINKLGYEVKKSQVKG"
     gene            17561..17980
                     /locus_tag="PKI50_08030"
     CDS             17561..17980
                     /locus_tag="PKI50_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03684.1"
                     /translation="MIVKPPNIVMIVQYDYIQPGDNAKEVGQVAKGDAEREKNGDVIT
                     FSRKKVMEEDGTYLVQTIRKLNPINERATDRGIAWFERFNNNELEIIKAYFEPDVNFT
                     YDSIAGKADATPESMAEGYKKATKMVVKDGKAEMTKY"
     gene            18204..19688
                     /locus_tag="PKI50_08035"
     CDS             18204..19688
                     /locus_tag="PKI50_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNT03685.1"
                     /translation="MNKIGFSSEKYLQMQSEKIKERIEFFGGKLYLEFGGKLFDDYHA
                     SRVLPGFEPDSKIKMLLQLKDKVEIVIAISADDIERSKRRGDFGITYDQDIMRLIDAF
                     REIGLYVGSVVITRYRPNFSTDSFQKRLNNLGIKVYRHYPIADYPAAIPLIVSEDGYG
                     KNDYIETARSLIVVTAPGPGSGKMATCLSQLYHEHERGIKAGYAKFETFPIWNLPVKH
                     PVNIAYEAATTDLNDVNMIDPFHLDAYGITAVNYNRDIEVFPVLNTILDKILGKSPYQ
                     SPTDMGVNMAGFCITDEEAVCRASEQEVIRRYYNTRCAQRQGLAEKAEVYKLELLMNT
                     LGITTECRPAVKAALKRAEATDGPAVALQLHDGRIVTGKTTSLLGAAAAVMLNALKEL
                     GGINHDMHLISPIVIEPIQALKLKHMGNHNPRLHTDEILIALSICATTNPTAELAIQQ
                     LSKLRGCEAHSTVILSRVDENVFQKLGINITCEPQYQSKRLYHK"
     gene            19707..20096
                     /locus_tag="PKI50_08040"
     CDS             19707..20096
                     /locus_tag="PKI50_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNT03686.1"
                     /translation="MYEMKQEYFTGIKFIDDEHTRLFAIANECYELLTNQFLPDKYDY
                     IVNIINELKDYARYHFKHEEEYMGSIGYKRILSQKVAHNDFIEKIDEYDSEKIDENQK
                     ESLLELLDFLNTWLIEHILKKDKQIVE"
     gene            20120..20710
                     /locus_tag="PKI50_08045"
     CDS             20120..20710
                     /locus_tag="PKI50_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HNT03687.1"
                     /translation="MIYRNAVIKDIPAISELQKKYHIATISEEDKPDGFVTTLFTEEQ
                     FKELIEKENGIAVAYDGDKIAAYAMAASWEYWSKWPIFQYMIEDLPNIEYMGQILSVK
                     NSYQYGPICIDKDYRGTEVLPKIFDFSRIQMKNRYPILITFINHINTRSYNAHTKKIG
                     LDVIKSFVFNNNNYYELGYDTSKKVRTIPENGGDRT"
     gene            20707..21339
                     /locus_tag="PKI50_08050"
     CDS             20707..21339
                     /locus_tag="PKI50_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03688.1"
                     /translation="MNKAKLKEAEERFLLKYPGGFSDPLMLEIAKKHKVDKMHKAAQE
                     SFEAENFDDPDKIVDSMSRIVGQSSLVSVFEKPKFRDLVKEISPGERALLSQGLREFL
                     HGDQASGFRLMTGLLDEYKLAKWPLLTVCPVYYRPDVEVFIKPTTVKGVIEYFELEGF
                     KYSPKPTYDFYRAYREQINRIKKEVDASLQIDNTAFCGFLMMSLEIPSYR"
     gene            complement(21364..21828)
                     /locus_tag="PKI50_08055"
     CDS             complement(21364..21828)
                     /locus_tag="PKI50_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT03689.1"
                     /translation="MPCPMMGCEADEYNSMELQDYGPEPFVVNIEEASKQNNNFRTAL
                     WTGRHLQLTLMSIKVGGDIGLEMHPDLDQFIRIEEGQGVVKMGERKDRLNFQRKVYDD
                     FAFIIPAGTWHNLINTGNIPIKLYSIYAPPQHPKGTVHKTKADAEAAEKKRG"
     gene            22267..>24344
                     /locus_tag="PKI50_08060"
     CDS             22267..>24344
                     /locus_tag="PKI50_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HNT03690.1"
                     /translation="MEKATKTLFLVVLLGCIFALNTYAAEPEEIYTAEQLNDVRNNLA
                     GSYILMSDIDLSDTVWGEVYAGEYGTSGWEPIGSQASPFRGSFDGNGYTITGLFIDKP
                     NVNEVGLFGNTAASATIEGLRLGDVNVTGKMDVGGLVGLNEGIITNCYVTGSVKGTAE
                     EIGGLVGYHKNAGTITNSYSACAVEGTHFLGGLIGRNEAPISNSYATGDVYGILAGTS
                     SAVGGLVGRNADGIINSYATGNVTGGSNVGGLIGYNWSDMNVEYSYATGAVEGTTKVG
                     GLIGSINVNGTGTVLNSYYNSCTTGQTEDDSGNGEPKTSTEMVQTDTFIDWDFTNTWN
                     INEWESYPRLKWSSHVPAPPAATAVTVSSASVELTYGGGPTTLTAAVEPVCAIQKVTW
                     STSNSDVATVDENGVVTPIGAGEATITATPVSGDVVGETAVTVGKKELTVSGTFTAGD
                     KVYDGSTTAVIDASSLTLSGVVGGDNVELSTSAVFANKNVAENITVNLTSDNSLIGDD
                     AGNYTLSLTGAPISTANITAMELTVINAAAQDKVYDGTADAIIDGAQLSGVVDGDSVT
                     LEDHTVGIFAQATVGTGIGVTTNMTITGDDADNYTIAQPGLLTANITAKELTVINAVA
                     QEKVYDRTTDADIINAELSGVVDGDSVTLENHTVGTFAQAAIGTGIGVTTNMTITGDD
                     VENYTIAQPVL"
BASE COUNT         7132 a         4734 c         6112 g         6366 t
ORIGIN      
        1 aggcctggag tcaggcagta ctacggtaac cgtaactgcc agcaaggagg gttatctccc
       61 tgcggtaaag agctttacag ttacggttgt tacagactat gaagaagaag gcgactggga
      121 taagagtatt attggcgatt atggagagta tacggatata gctctggata gtaaggggat
      181 gcctcatatt atctttgagg ctagctcggt ggagtaccgg cactggaacg gcagttcttg
      241 gcaagaagag gaagaacccc agattggcag cagtcaggga tctttagcca ttgttaagat
      301 tgacggaaat gaatactcct taatttctta taatttaaac ggctgctttg gatacgatgt
      361 gttcgacgga gagtattggt cacaccagag ttttggggat gaaggagtga cagttgcggc
      421 ttccagcatt gatacaatca tgtacaacgg tgagtatggc accggagtca gttattatga
      481 tgaggaaaac ggtgatttgt acttcaggtt cgatacagat ccaatagata ttattcttga
      541 ttcttggggt gatcctttcc cagtggacgg ggaaaacagc gatgcgggaa aatcttcttc
      601 cctggtcttc gatgaggatg gcctggcgta tattgcctac tatgattata gcagcggcgg
      661 ggaggacggt tccctgaagc ttgccgtaat taatgacctg ctggatcctg aacttgatat
      721 aatggaagtc gatgaccagg tgggatacga cgacggccag tatgtttctg tttctcaggc
      781 tgtttataat gaatcaactg caatacatct ggcatattat gatgccgtca ataaaaatct
      841 taagtacgcc atgtggttag acgaagagtg gttcattgaa acggtggatg acagtgaagg
      901 cgatgtgggc aaatatgcct cgctggttct cgattatgat gggaaccctc atatcagtta
      961 ctatgctgag agtgattcgg gaggagcgct gaagtacgcc cgccgcgatc cggagcatga
     1021 gggagaatgg ttgatagagg taattgatca gggcgaggaa gtagggctgt acagctccat
     1081 agcctttgac aactattttt cgataccgca ttttgcatat acagccatga ccggtgggga
     1141 ccgggtagtg aagcatgcca ccaggcaggg tatcatgaat accattgagc caggtgtact
     1201 gtccgggact gcggagacat ttgaggatgc tgacttcctc attgaaagct ttgaaaatga
     1261 aatttgttat gtatggagag aagtgtgggg agagatagat tatttggggg atgaagatta
     1321 tattatttct gaagatggtg atgtattaac aattaccctg aaaagcgact atctcaacag
     1381 cctggagtat ggtatccaca aaatatggtt ttacttcgat tacggcttgc ctgttctctt
     1441 aaccattgat atagaggaaa acttggaagc ttatacatta aggtctaacc cggatctggc
     1501 ttatgatccc atgaataacc gctacttaat ggtttacgag aaaatcatag gtgacactaa
     1561 tcccggaata tggggcaggt ttataagcgc tgacggcgaa caagacactg agtttccaat
     1621 atccgcggag atttactgca atgagcctaa agtagtatat aatcctgtga ataataattt
     1681 cctggtaacc tggtcgcatt atcccaacta tgaggagcct ttgatatatg cccagatcct
     1741 ggacgatgaa ggggaaccct acggggattt tgggaatttt gccgtgtgtc cggaaacaac
     1801 gggcatgggc tatggtcagg ggcggccagc agtggctgtc aatacatcca acggtgacta
     1861 tctgattgcc tgggaagagg aggcaggggg tgacctgcaa gacgatatct acgggcagct
     1921 tcttgaccag gatggtgtgc agattggcga aaagcagacc ctggtaagca tggactggta
     1981 ccagtctgat tcggttcttg cctacagtgc tgccggcaat aagtatctgc tggcctttgt
     2041 ggacagcaat gatatcctgg gttggtacat cggggcagac ggggtgccag aaggagccat
     2101 tcaagagatt gcccttgatc aaaatcggcc tgatgaagaa caatttgatc ctgatgtcgt
     2161 ggcggattct gacggccgta tcctggtggt ctggggggac aatcgcaatg aagactggat
     2221 aagcaatata tacggcaggt tcttcaatac cgacggaaat atggaagatg aatttattgt
     2281 ttatcagtca gaggtagatc aatacgagcc gagtgcggct attaatactg cagacggctc
     2341 ctgtctggtg gcatggcggg atgcatacga caggttatat ggccaaaagt tcactgcaga
     2401 tggtaccgga attggggagc ctgttgaaat tgattctgat ggagaaggct ttggaaatgc
     2461 tgcaatagtc tataaccctg aagaaaacaa ctacctggcg gtttatgaag actataatag
     2521 ctacccgcat aaaataaagt ataaaatact gtatccgcag cagccttaat tataaaccgc
     2581 agcagctgcc cgctgataga aaaattagtg aggtaacggt atgactttga gtgattttac
     2641 tataaccttt tggggtgtca ggggatcccg gccggtgccg ggacccagga cagtcatata
     2701 cgggggcaac acaccctgtg tagaagttag aatcggcaag agattaataa tcctggatgc
     2761 gggaacaggg attaccaacc ttggctccaa gctgatgcag gagccgggcc ctgtcatcgg
     2821 ggatattttt atcacccatg tacattggga tcatatacaa ggttttcctt tcttccagcc
     2881 cgctttccgc ataggcaaca gcttttgtct ttacggagaa aggaagggaa atgtttcttt
     2941 tgcggcatca attaagagaa ctatgagaca tccccacttt cctgttcccc tggaacacat
     3001 gggggcagcc ctgcatttta ccgaaattga acctggggat atgctggaac tgggggatgg
     3061 cattaccgtg cggacagccg caaacaatca tcctgacggg ggaatctcct accggattca
     3121 gtacggaaat aaatcctgct gctatgtgac tgattacgag catggcccgg aaaaggcgca
     3181 ggcactcctg gagctgtgtg ccggaacgga tctgctcatc tacgatgctt gttttaccgc
     3241 tgccgagtat cccggctatc aaggctgggg acattccact tgggaagagg gattggccct
     3301 ccttgaggca acgggagccg gccggctaat cctcttccac cataagcccg agcgcagtga
     3361 tgaggaaatg gcggttctgg aaacgaatat aaccggtcaa tatccaatgg tcaaagcggc
     3421 cagggaagga atggtgatta gtttatgagt atcgccaacg ccaagctggg cgatcatctg
     3481 gaggaactgc tgaatatcgg aattgccctg tcggcggaga agaatcataa caagctgtta
     3541 gaacggattc tgaaggaagc ccgcaggatt accaatgcag atgcaggcac cttgtattta
     3601 tgcgagcagg atcacctggt ttttaagatt attcataatg ataccctggg aatatatcag
     3661 ggagggcagg gggagcccgt tgatttacct cccgttcccc ttgaccctgc caatgtttct
     3721 tcctgggcgg cattacacaa gaaaagtgtc aacatacccg atgtttatgc tgccgacaat
     3781 tttgatttct ccggtcccaa gaagtatgat gctatcaccg ggtatcgcac cggctccatg
     3841 ctggtggttc ccttggagaa ccatgagggg gaagtgaacg gtgtccttca gcttttgaat
     3901 gccaaaaatg aaaagagcca aatcataccc tttgcccccg aactggagaa gattgttttt
     3961 tccctggctt cccaggcggc cgtatcctta actaatatgc agtatattca agagattgat
     4021 aatctttttc attcttttgt ccaggtcatg gctacggcta ttgatgcccg taccccttac
     4081 aatgtcaacc atacccgcaa tattgcccga ctggcggaaa aatttgctgc ctatattgac
     4141 agcttggccg gcgggccgtt cagcagcgaa aaatttcccg agagccggac agcccaactg
     4201 gtcatggcgg catggctcca tgatatcggt aaggtggcta tccctttgaa cgttatggac
     4261 aagcccagcc gattggggga gcggattgag tatgtgcgac agaggctgga actggtggaa
     4321 aaccgcctca tcaggtcttt tctggagaaa aaagcggccg gggcagccgg cagggaacag
     4381 gaagaggggc accggcagca attggatcgt ctcagccaag cccgggaact cattgagaaa
     4441 gccaatcacc cggcaacttt tgtggatcag gatttagtcc gggagttgtc cgagctggcc
     4501 caatgggaac ctttgccggg agtgaggctt ttaatggatg aggaattcaa atgcctcagc
     4561 atacccaaag gcacactgac cgctgaagaa cgaaaggtca tggagagcca tgtggctgtt
     4621 gcccaaagga tgttggaaaa tatacgcttt aacagccgtt ataaagatgt gcctgcttgg
     4681 gccttgagtc atcatgagtt gctggacgga acaggctacc cccaaggctt aaaagccggg
     4741 gaaattccct tggaagccag gattcttgct gttctggatg tctatgatgc tttaacagct
     4801 tcggacaggc cttataaaaa ggccatgcct tccaatgcag ccttaaacat cctggaaaag
     4861 atggccgagg aggggaagtt ggataacctg cttgtggcgg cttttaaaga gagccggatc
     4921 tgggaggaat aaagggagtt gtgctgatga aaaacaatat ttttaataaa aaatacattt
     4981 tgatattttt gctttgcatg attcttggct tttctgccta tgtggatatc ttcaccactt
     5041 tggacagtaa aatgcaggac agcttcctga ggaaaagcgg ccctgtagat acggaaattg
     5101 tcctggtggc tattgatgat cagagcttgg aggatctggg ccgcttccca tggccccgct
     5161 atgttcacgc agaactatta aggatcctgt cccaggctca gtcggcagcc atcggcattg
     5221 atataatttt cagtgaggct gccgatgatc cggaagaaga cctggctttg gcagaagcta
     5281 tccgggaggc aggcaatgtt gtcttgcccg tctatgccgg cttggagggc ttttccgcca
     5341 ggcagggagc catcaaaacc gataatctgg tggttccttt tccggacttc cccgatttat
     5401 tcattaccgg gcatataaat acttttcccg acccggacgg aatcataaga aagaccgccc
     5461 tgtatctgga ttaccagggc caaaccgttc ccagcttcgc ttggcgtatc tatgagcagt
     5521 attgtaagag caaggggctg cctgctcccg acctgaaccg gctgcccctg gatcccatca
     5581 aaaggctgga aataaactat gcgggcattc ccggcgatta tgagcatgtc agttactccc
     5641 aggtaataag cggagaggtg cctcccgaat tcttccgggg cagaattgtc ctggtgggtt
     5701 cctatactgt gggaatagga gatttttact ttactcccct ggccccagag gtacctatgt
     5761 atggaatgga aattcatgcc aatatcatac agaacctgct ccggggcagc tatctgcaga
     5821 gagcgccttt cagtgcttcc ctgctggcca tagtcatttt tggggcagcg gcagcttttg
     5881 gctttacccg gctcaacccc caaaagggcg tgctgctcct ggcagtactg gcagcatgct
     5941 atctggccgt cgcccgactg atgtacgaca aggggtatct cctttctttg ctttatccct
     6001 tgctcctgct ggctttgggc tatttggtgg ctttggcagc taattacatc agcgagttgc
     6061 tggaacgccg ccgcatcacc ggtgtcttca gccgctatgt ggttccccag gtagtggaca
     6121 agctcctgga gggcggcgaa aaagctttgc agctgggagg aagcaggcgg gagattacag
     6181 ttctgtttgt agatattcgg ggttttaccc ccctttcgga ggctgccgcc cctgaagagg
     6241 tggttgaaat cctcaacgaa tacctgacct tggtggccca gtgtattttt aaatacggag
     6301 gcaccctgga caaattcatc ggggatgcgg ccatggctat gtttaatgct cctttggatc
     6361 tggaggatca ctgcttaaaa gccgtccaag cggcttgggc gatgaaggaa ggggcaactc
     6421 ccctgcagca gaggctggaa gaaaaatttg gccgcactgt ccagttcggc ataggggtga
     6481 acacgggacc ggctgtcata gggaatatcg gagccagctt cagaatggac tatacagcca
     6541 ttggcgatac cgtcaatacc gctgcccgtc tggagagcaa tgccaaaccg ggccagattc
     6601 tgatatccca ggacgtatac gaaaaagtaa aggaaagagt agcagccgcc aacctgggcg
     6661 agatcaaggt caaagggaaa gcccagggag ttaacgtctt ccaggtcgat ggtgtgcatc
     6721 agtaaagctt ttgttatgga ttgtgtatat gaccgtatcg agatttcacg gacaggggtg
     6781 accctgtccg tcaattatga gtgcagcaag ttgattaatg gcacatctgc taatggtgaa
     6841 aattcaggga gaggaatatc agggaaatga cacattttaa acaattatca gaagtactaa
     6901 gaaattatta cgatatcccg gatcaggaat tggacaaaat aaacagtttg ttctatccat
     6961 gcaaactaaa aaaaggagaa tattttgtaa gaagcggtta tatggcagat tcaatcggtt
     7021 ttatcgtgac cggattattg cggtattttt atactgacaa aaatggagta gagtatacca
     7081 aggcttttgc ggcagaaaat gattttgcgg tatcatatgc aagtatgctt ctgaagaaac
     7141 cttcggcttt tcaattgagg cattggaaaa taccgaattg ctttgcatca attattcaga
     7201 ctgggagaag ctgctggaca atgtaccatg ttggaatatt gtcgctcgaa aggttgtaga
     7261 aaaggtatat attatgaagc agatgcgcga gatagaatta cttcttgagg atgctcaaag
     7321 catgtatatt cattttatgg aacagtaccc tatgctgcat aaaagactaa aacagcatta
     7381 tattgcttca tatttaggta tttcaccggt caccttaagc agaattcgca gaaaaatgag
     7441 taattattaa cctatattaa tgaaaataaa acctctgact atatactcaa gctatataga
     7501 ggaggtagat acaatgaaca caaagaacaa tgttgtttta tattttgagc cgtgtaaatc
     7561 cgcgatattc agagaataaa gcaatggcag aaacggcagt gtttgtactt aattgagcat
     7621 tctataaatt catttacttg aggagtttct taatgaattt tctatataga ttctctcttt
     7681 tttttcttaa tataactccg gcttcaaggc aaactggaaa tgtgattatt gggaagagag
     7741 tatagtgaga ggctttcaca ccgtccagtc cgacagtaca gtatacggat attacgcctg
     7801 gaactccctt atcctggtag gcaaagcgga gggcaacaaa ctcagcggac atatatacga
     7861 cggtattttc gaaataagga tgaaccctga cgggaaatcc tactcgggtt tcatatttga
     7921 taacagtgcg gcagaagccg gttattggac gggtatcagg aaataataaa cccgaatgca
     7981 tagatgcacc gcaaggtaca ttttttttgt ttactttatc tgaagggtgc tatatggtat
     8041 aatcctcatg ggagaactga tcaagtatat taatagagta ataaagtaag gagatggcgg
     8101 agtggaatgc agttataatt caaatgccgt agagattgaa gtgataaaag tcaatggttc
     8161 ggagagcaaa attaaaaatg agcaggttgt aacagaacgg gcacttactg tctacctgaa
     8221 cggacaggag tttgtgacaa tggtctattc tccggggcat gaaaaagaac tggtaaccgg
     8281 ttttctggct ttggaaggca tcattaagag gcccgatcaa ttggtcaagt tttcttatga
     8341 tatgcaggaa gggctggcct gggcagacac tgcggaaaaa agtattttag gggaaaccct
     8401 gtttttgaag cggtatatga catcctgctg cggcaaaggc cggacatctt tctactttgc
     8461 caacgatgcc aggcttgctc aaaaaattga caacccttta caaataacct ccaaagacgt
     8521 cagttactat attaatctta tggaagaaaa gtcggaattg tttcgccgga ccggcggagt
     8581 acacggaggg gcgttggctt ctgacggaca gcttcagtat tttgcctttg acattgggcg
     8641 gcataatgtt ttggataagc tatacgggca agcttttttg gagggtatcg atatctcccg
     8701 gaaaataatt gtttttagcg gccgtatttc ctctgagatt ttgataaaaa cggccaaaat
     8761 gggatgcccg atattgatag gagtttccgc tcctaccgat ttggcgcttg acctggccaa
     8821 agagcttgga attacagtta ttggctttgc cagacaggag cgtatgaatc tatacacaca
     8881 tccggagcgg attataattt ccgaagagcg gtctttttag aagataggtg acaggcccct
     8941 aacttatctg cttaattata ggggaattag tgtcaagcac gaaggttaaa ctgttattga
     9001 atgattggca gccttctggt attatgtagg cagcatatac caggaggcaa tttttatgga
     9061 gaggccttgg agagaggaaa atgaaatgaa taagacatat aagtatgagg ccgccattca
     9121 gaaagtcccc gacatagacg gtgcatatgt cgaatttcct tatgatgtga aaattgactc
     9181 aattatatta atgagtcttc atcagaaacg gaatgatata ttcggcggaa ttggctacca
     9241 catgcccgca attgggaagc aaatgaatta cagctgatgg tataaggcgg gagagccttt
     9301 gcgctgactt ttcagcatca ataatagcat cattttctcc atctataaag agtatcggca
     9361 tgttaagcct cagtaattgt tcatcagcaa atacaggcag ctcccgaatc ggattgtagt
     9421 tttcaacaat taaattcata aaatcgagga cttcctttgg aatgctttgt tctcccatta
     9481 tggaagggct caccggtaca gttccattcg tctgccgcgt ttggtccgca ttaataagaa
     9541 attgcggacg gatctgtgca agtccgccgg atgctatcag aatcagcttg gaaacgcgtt
     9601 ccggataagc tgtggcaaat ttgagggcca tccagccgcc cagtgagttg ccgattaaag
     9661 cggctctttc aaggccgagg gtatcaagga catccttcat ccagagtgca aaagcgtttg
     9721 agcatagatc gggcctgtat tcctcgctgt ttccggcttc accgataatg tcaacggcat
     9781 agactctgaa ataattggat aaggccatga tttcgggaaa ccagaacgca ctgttgctgc
     9841 aggagccgtg cagcagaata atgggcgggt tcgactcatg gccggcggtt agcataaatg
     9901 tccgtccgaa ggtggtctcg acgtatcgct gtccgaaggg gaactgatca agcacgcggt
     9961 tataataagc cctaaccttg tcccgtcctg cctcagtttt gaatacgctt ttcttcatat
    10021 caatcactcc ttttcattat gttctgtcag tgaggctatc gtgtccatca tcactgccat
    10081 aaagcggatg tcaccccaga gtgtaacgcg aaccatattt tcaccctcga tatattgcga
    10141 ctgcatggac gattggccgt attccggcgg tttggagttc agggcagctg tcagtgtatt
    10201 catgatacga atggcatcct cttttccgcc agcagtgata tcgataaccg acgaggcgct
    10261 gatgcaatgg acaggccggt ttccggaaca ggatccatcg tcaccgttac tctcgacaaa
    10321 tgtgcttaaa tcatggcccg tgatgcgcca gcccttgcca acctttgatg cacgcagttt
    10381 tccctcccgg atataccgct ggatggtctt aggatgaatg tccagcatgt ctgaaatctg
    10441 ctggacggta taataacctt tcattacata acactcccta tagttactta taaggtaata
    10501 ataaggaata atgagaaaca tgtcaagaag tttttttatt tgccattgtt tccgcaagaa
    10561 aatacatgat atcataaata tagcattaaa tgcaaattac tatcatatac gaataattaa
    10621 gggatgcgaa gaatgagaag gattatttct ttatggaaaa gttagttatc ggaatattgg
    10681 cacatgttga tgccggcaag acgacgttat cggaaagtat gctttatgta agcggaaaaa
    10741 tcggaaagcc gggaagggtg gataataagg atgcctattt agacacctat gagcttgaaa
    10801 gggcaagagg aatcaccatt ttctccaaac aggccgtatt agaaataggt gagactcaga
    10861 taattttaat ggacaccccg gggcacgcag atttttcggc tgaaatggag agaacactcc
    10921 aggtgctaga ctatgccgtt ttggtgataa gcggtgcgga cggagtacag gggcatacca
    10981 aaacattatg gcggctgctt gatatttatc agatacctgt ttttatattt gtcaataaaa
    11041 tggatcagaa agggactgat aaggacaagc tgatggaagg catgaaaaag cagcttaatg
    11101 acggatgtat agaattcgga tatattacgg cggctgagtt ttacgaacaa ttggccatgt
    11161 gtgatgatat tattatggaa gcgtatctga aaacagggca tattgaagct gagcagatca
    11221 ggaaggctgt ccggaggcgc aaagtatttc cgtgtttttt tggttcggct ttaaaattgg
    11281 aaggcattgg gcaatttatg caaggtattg caaagtatgc aacggcacct tgctatcccg
    11341 atgaattcgg agcaaagata tttaaaataa caagggatga acaaggaaac cgtcttaccc
    11401 atcttaaaat taccggcgga aagcttaagg ttaaggatgt attggccggc ggcatccgga
    11461 aagaaaaagt aaatcaaatc cgtatctact caggacagaa gtatgaggca gttagtgaag
    11521 cagaagcggg atctgtatgc gcagtaacag ggctcagccg gggcaagccg ggagaaggct
    11581 tgggaataga agcagccgca ccggcccctg tattggaacc ggtactgatt tatcggatcc
    11641 tgcttcccga aggcagcgat ccgagagcga tgatgcccag gctgcgccag attgaggaag
    11701 aggagccgga gcttaatatt tactgggatg aacagatgca ggagataaag gtacgtatta
    11761 tgggggaagt gcagcttgag attctgcaga gcctgataca aagccgtttt ggagtaccgg
    11821 tgtctttcga tgccggaggg attgtctata aggaaaccat tgccgatgta gttgaaggag
    11881 ttgggcactt cgagccctta aggcattatg cggaggttca tctgttattg gagccggggg
    11941 aaccgggaag cggcctgcag tttggaacgg aatgcagtga ggatattttg ggcataaact
    12001 ggcaaaggat tattttgacc caccttcagg agaaagagca tataggggtt ctgacagggt
    12061 cggtgcttac ggatgtgaaa atcactctcg tttcaggcag agcccacaat aagcatacag
    12121 agagcggtga cttcagggag gctgccaacc gtgcggtgcg gcagggctta aggtcggcgg
    12181 aatccgtatt gctggagcct tattatgcat ttcagctgga gctgcccgag aaaatgatag
    12241 gcagagccat gagtgacatt gagaaaatgc acggaaccgg cgaattattg cagacggacg
    12301 gcgaaaccgc agttcttgcg gggagtgctc cggttattga aatgaggaat tatcaaaaag
    12361 atgtaactgc ctataccaaa ggtatgggaa ggctgttttg cagcctgaaa ggttacgggc
    12421 cctgccacaa tacggaagag gtcatagcaa gcatcggata tgatccggaa agggacgcgg
    12481 agaatcccgc gggctccgta ttttgcacac acggggcagg attttcagtg gcttgggatg
    12541 aagtaaagaa ttatatgcat gtagagagct atattaagaa aagggatgac ttttccgggg
    12601 aagcagcaac agaaaaagca gcatcggaag agggtcggat aagcttggaa gagatcgacc
    12661 aaattataaa taaaacctac tatgggaacc aagggaaaaa atctgcctgg aaaagactca
    12721 aaacgacccg ggaaaactat tgcgggcctg ttgaccgtgt cagaagggaa aaggaaccca
    12781 aagaagagta tctgctagtg gatggctata atattataca tgcatggcct gaactgaaag
    12841 agcttgcgga ggtcgacata ggaagtgcca gaatgtcgct gctggattct ctcagcaaat
    12901 accagtcggt tcggcaaagc aatgttattg ttgtattcga cgcttaccgc gttcaggggc
    12961 accgggagga aatgaccgac taccataaca tccgtctggt atataccaaa gaggcacaaa
    13021 cggcggacca gtatattgag aaatttgccc gggataatcg ggataaatat gatataacag
    13081 ttgctacatc ggacggtttg cagcagataa tcgtaagagg tgcggggtgc tctctgttat
    13141 ccgccagaga actgaagatt gaaattgaaa gaaaggaaaa gtcctatgga caagataatt
    13201 gagatactgg aaaaccaggg acctttaacc ggtaaggaat tgcttgaaaa aaccgggatg
    13261 gacgaattta ctgcgtggaa aaattgtaat agcagtgaaa gaatagtcac aaaaacaata
    13321 ggaaccagat acctgaggct tgacaggctg gttgcaggat atgccaggct ttcaccttcc
    13381 atcatcaggg agttttttgg ctacacagta atcggcactg aaaagaattt gccggatata
    13441 ctgaggaagg ctgaatatct cagtcaggag attatccgga taagcaaaaa caaatttgag
    13501 ctggcgaaag agacaataac aaaaatcgtt gaatcccagg cagcttcaaa tattataaaa
    13561 ggaggtgcat gctttattat tgcgggggat gtggcatatg aaatgtcgca tctggagccc
    13621 cggcccgaag tgtccaccgg tgagctggtt aagggttccg accttgatat tgttgcggtt
    13681 acccaaggtt tgccgggcaa tataattaat gcccttgata cttccatata cgaacacaag
    13741 tactacctgc ttaagaaccc cagttataat gaagagatag attatatcgt aaaagacatt
    13801 tcaaaggtaa aagaacagct gaaattcgat agctttgaat cgatggttgc ttcaaaaatt
    13861 ctttacgaag gtaaatttct ttatgggaat tttgatattt tcaaacagat taaaaaaatg
    13921 cttatagatg aaggcatacc tgaaaaaata agagttctgg aggagaaagc cttgctcaac
    13981 agaaacaatg ccaagacaca gctgttaaaa tatgaaggta aactgactga tgaagagagc
    14041 atgaaattgt tttacacaaa ggaagagaaa gaggaattct tctgatgaat tcctctaaaa
    14101 accatattcc tgttttaatt tttccacatg catatagtga tgaaggcctt tgagctttga
    14161 ggaggaatcc ttatcaagga ctactgtaat tccgccgctg tgcagttgta ttgccgaagc
    14221 ggtgatttgg gaagtaacag ggccttcaat gcacttggca actacgtcag ccttcttgat
    14281 tccgtttgca agcattatga tatttttagc ctctaaaatg gttccaatgc ccattgttat
    14341 ggcaaagtgc ggcatatggg ccctatttat gccggctttc ttgtagaaac tctcataatt
    14401 atcatcaagg gtttgttgtg taagggcttg aattctggtt ctggatgcaa gggatgaccc
    14461 gggttcgtta aatgcccagt gcccgtcgcc gcctatgcct agaagctgta aatctatccc
    14521 gccggctttt ttaatttctt cttcatacca tttgcagaac tcttcaggtt tctttgtcaa
    14581 tccatcggga atatgtatat tttctttttt gttattgata tgcttaaaaa gttcctcatg
    14641 cataaatctt gcataactct gatccgattc gtagggcttg tccaggtcaa tgcctatgcc
    14701 gtaatattca tcaagattga aagtcctgac ctgtgaaaaa tccagtcctt cctctttgtg
    14761 cattctgata agttccttgt aggtacctat gggggttttt ccggttgcca gtccaagtac
    14821 gcaatcaggc tttgatctta ctaaacgggc aataagctta gctgaatatt tgcttagttc
    14881 atcatagttt tctcttacga taatatccat tttaaacctc tcttcaaatg attgtactta
    14941 tattatatat tttctgttct atattttcat taagattatg aaaaaatttg aaaaaataaa
    15001 taattttgat ttaggtcaat ttattttcct taatttccgg atataatgga atcaacaaaa
    15061 cagataaaat tataaagaaa gaagggaaat acatgtcaaa ccggacaata tttgatgaaa
    15121 tcaggaaaaa tctattccca aggctggaac aatatgtacc tgttgtagcc cgggttcacg
    15181 gaggcaatca tccggaattt cacgaagttc gcagattatt tgatgagata ataaagaaaa
    15241 caaaaaaagc ggcaccggaa aaacctgaac taagcgaaga atttgccaga ttgcgtgaaa
    15301 ttaccaataa ctatacggtt ccggaagatg tatgcgaaag ttatgaagca gtatacaaca
    15361 tgctggctga ggcagacaaa gcataccagg cttaaacgga atgattatcc ggaatgattc
    15421 aaggaagaag gtgaagaaga atgcagcgat tcatattaag taagaaaaat cacatagcat
    15481 tgattagtgc aattttaatt acaattggat ttaccggtga actgggtttc aaaaatgaag
    15541 ctgtggcggt ttggtccttg actgccgcct cggttttagg ggctgcacct attgcaattc
    15601 aagcctatca agcgctgaga gttaaagtcg tcagtataga tgttttggtt accattgcag
    15661 ttatcggggc attcttaatc aagaaccttg aagaatcagc cgtagtcaca ttcctgttct
    15721 tattcgggtc ctttttagaa cagcggacac tgaacaaaac acgttctgcg ataaaagaat
    15781 taactgagat ggcgccggaa agtgctttga agcaaatgga aagcggcgaa tttgaagaag
    15841 tggatgtgga tgaagttgac gtgggggata ttctgcttgt taaaaccggc gcaaaggttc
    15901 ctgttgacgg tacggtattg accggagaag gccatgttaa tgaagcaagt attaccggtg
    15961 aagctgttcc ggcaggtaaa gagaaaggtt ctcaggtgta cgcggggaca atattggaaa
    16021 acggaacaat tcaaattgta gctgaccgtg tcggtgaaga taccactttc ggcagaatta
    16081 ttgaactggt tgaagaagca caggattcaa aatcagaagc cgaacgtttc attgaccgat
    16141 tctccaaata ctacactccg gcagttttag ttctttcaag cattgtatgg ctgatttcac
    16201 gggatattga actggcaatt acaatattgg ttctgggatg cccgggagca ttggttatcg
    16261 gcgtgcctgt ttcaaatgtg gcgggtatcg gcaacggagc acggcacggt gtccttctga
    16321 agggcagtga agttattcat gattttagca aggttgatac aatagtattt gacaaaacag
    16381 gtacattaac aataggaaat cctaaagtag ccgacaaaga aatttatgca gataacgccg
    16441 atgaagtatt aggttatctt gcaagtgttg aaagtgaatc aggccatccg ttggcaaaag
    16501 cagttgcgga atatataggg gatactaaag cttatacagt tgaaaaaaca gatgttgtaa
    16561 aaggcggcgg aattgtcgct caagcagagg ggcatagagt agcggtgggc aatgctgcgc
    16621 tgatggagca ggaaggtgtt catttggatg aaaaagcccg tgcggatatt gcccgattcg
    16681 agaaaaacgg aaactcactt gtcctgacat cggttgatgg tgaattaaaa gcactgatgg
    16741 gcattcgtga ccaaattcgc ccgggggtaa aagaagatct tcaaaagctg aaaaaattag
    16801 gtgtcaaaaa tctggtgctt ctttccggag ataaccaggg aacagtggat ttagcggcac
    16861 gtgaattagg acttaccgaa gcccatgggc acatgctgcc ggaagacaaa tccacatata
    16921 taaaagagct gcagtctaaa gggcaaattg tcgcattcgt cggagacggg gtaaatgaca
    16981 gtccttcaat agctctggca caaatcggga ttgctatggg cagcggtacg gacgcagcga
    17041 ttgaaacttc ggatattgtc ttaatgaatt cggatttcag ccgcttgccc catgcattag
    17101 gtttaacaaa agcaacagcc aataacatgc gccagaatat tgttatcgcg gtgggagttg
    17161 tactggtcct gcttgccagc gtctttttca gcgaatggat gaacatgtca atcggcatgt
    17221 tagtacacga agcaagtata ttggccgtta ttttaaacgg tatgaggctc cttagttaca
    17281 gactgagata ttaatgcttt gcagattaat aaagattaaa ggagagatga aaaatgaaaa
    17341 aggcagctat tcagttagaa acattgactt gtccctcgtg tatgctaaaa atagaaaatg
    17401 cggtaaaatc tttagaggga gtagaaagag aaagcgtaag cgtttcattc aattcaagca
    17461 aagtaaaact ggactttgac gacgaaaaaa tatcaataaa agatatcgaa aatgcaatca
    17521 ataagctggg atatgaggtt aagaaatctc aggtcaaagg atgattgtca agcctccaaa
    17581 tatagtcatg attgttcaat atgattatat acagcccggc gacaatgcaa aggaagttgg
    17641 acaagttgcc aagggtgatg ccgaaagaga gaaaaacgga gatgtcataa cctttagcag
    17701 aaagaaggtt atggaagagg acggcactta tttagttcaa actataagaa agctgaatcc
    17761 aattaacgaa agggcaacag acagaggtat cgcttggttt gaacgcttta acaataatga
    17821 gcttgaaata ataaaggctt actttgagcc ggatgtaaac tttacctacg attccatagc
    17881 aggaaaagcg gatgcaacgc cggaaagcat ggcggaagga tataagaaag ccactaagat
    17941 ggttgttaaa gatggtaagg ctgaaatgac aaaatattaa taaagctgtt atgataaagg
    18001 actgcctaca ggggcagtcc tttattatgt gaagtaaaat ttcaatcctt tttatcaaaa
    18061 aaatgcgatt agcagttgta tttcgacatg ctttcaatat attattatat aaggaggaaa
    18121 aatacttata gttattcatc aagataattt ggaataaata tgagtagctt atattaacaa
    18181 aagtattgta caggagaata tatatgaaca aaattggatt tagcagtgaa aaatatcttc
    18241 agatgcaatc agagaaaata aaggagcgga tagagttttt cggcggtaag ctgtatctgg
    18301 agttcggcgg aaaactattt gatgattacc atgcttcccg tgtacttccg ggctttgaac
    18361 ctgacagcaa aataaaaatg ctgctgcagc tcaaggataa ggtggagatc gtaattgcaa
    18421 tctcggcgga tgatattgaa agaagcaagc gacgcggcga ttttggaatt acatatgatc
    18481 aggatataat gcgcttgatt gacgcatttc gtgaaattgg attatatgta ggaagcgttg
    18541 taattacacg ctaccgccct aacttttcca cggattcctt tcaaaagcgc ctgaataatc
    18601 tcggtataaa ggtttacagg cattatccca tcgctgatta tcccgctgcc atacccctaa
    18661 ttgtgagtga agacggatat ggcaaaaacg attacattga aacagctcga tctcttattg
    18721 ttgttaccgc ccccgggccg ggaagcggta aaatggcaac ctgtctctca cagctttatc
    18781 atgagcacga acgcggcata aaggcgggat atgcgaagtt tgaaaccttc ccaatatgga
    18841 acttgcccgt aaagcatccc gtcaatattg catacgaggc agccaccact gacttgaatg
    18901 atgtaaacat gatagacccg ttccacttgg atgcctatgg tattacagcc gttaactaca
    18961 atagggacat tgaagttttt cccgtgctga ataccatact ggacaagatt ttagggaaat
    19021 ctccttatca aagcccgacg gatatgggtg taaatatggc gggtttctgc attactgacg
    19081 aagaggctgt gtgcagagca tcagagcagg aggttattcg caggtactac aataccagat
    19141 gtgcccaaag gcaaggcttg gccgaaaaag cggaagtata taagctggag cttttaatga
    19201 atacattggg tattacaacc gagtgccgtc cggcggtgaa ggcggcgtta aagcgcgcag
    19261 aagctaccga tggccctgct gtagcgctgc agcttcatga tggacgtatt gtaacgggaa
    19321 aaaccacctc tttgcttggc gctgctgcgg cagtaatgtt aaatgcattg aaagaattag
    19381 gcggaatcaa tcatgatatg cacctgattt cacccattgt aattgaacct attcaggctt
    19441 tgaaactaaa gcatatgggc aatcataatc ctcgtctgca taccgacgag atactgatag
    19501 cattatccat atgcgctacg acgaacccca ccgcagagtt ggcgatacag cagctttcca
    19561 agctgagagg ctgtgaggca cattccacgg ttattttatc cagggttgac gaaaatgtgt
    19621 tccagaagct gggcattaat atcacctgcg aaccccaata ccagtcaaag aggctctacc
    19681 ataaataatc tttggaggga tgtattatgt atgagatgaa acaggaatac tttaccggaa
    19741 taaagtttat agacgatgag cacacgaggc tttttgccat agcgaatgaa tgctatgaat
    19801 tgttgaccaa tcagttcctg ccggacaagt atgactacat agtgaacata ataaatgaac
    19861 tgaaggatta tgccaggtat catttcaagc acgaagaaga atatatggga agcatagggt
    19921 ataaacggat tctctcgcag aaagtcgctc ataatgattt tattgaaaaa attgatgagt
    19981 atgattctga aaaaatagat gagaatcaaa aagaatcgct tttggagctg ttagatttct
    20041 tgaatacgtg gctgatagag catatattga aaaaggataa gcagattgtg gagtaaaggg
    20101 gcggaaaatg aaaaaagata tgatttatag aaatgctgtg attaaagata tacctgcaat
    20161 atcagaattg cagaaaaaat accatattgc aaccatcagc gaagaagaca aacctgatgg
    20221 atttgtgact accttattta cggaagaaca gttcaaggag cttatagaga aagaaaatgg
    20281 catagcagtt gcctatgatg gcgataagat agctgcttat gctatggctg cgtcctggga
    20341 atactggtca aagtggccga tttttcagta catgattgaa gacctgccga atattgaata
    20401 tatggggcag atactttccg taaaaaactc ttatcaatac ggcccaatct gtatcgataa
    20461 ggattacagg ggaacggaag tacttccgaa gatttttgat ttttccagaa tacagatgaa
    20521 aaacaggtat cccatactta taacatttat taaccatata aataccagat catacaatgc
    20581 ccatactaaa aagattggcc ttgatgtaat aaaaagcttc gtgttcaaca acaacaacta
    20641 ctatgaattg ggctatgata catcaaaaaa ggttcgcacc attccggaga atggagggga
    20701 tcgtacttga ataaagcaaa gcttaaagag gctgaagaaa gatttttact taaatatccc
    20761 ggaggatttt cagacccgtt gatgcttgaa attgcaaaga agcacaaggt ggacaaaatg
    20821 cacaaagcgg ctcaggagag ctttgaagct gagaatttcg atgacccgga taaaatagtt
    20881 gattcaatga gcaggattgt cggccagtct tccctggtat cggtttttga aaagcctaag
    20941 ttcagagacc ttgtaaaaga aataagcccc ggtgaaaggg cgcttttatc ccaagggctg
    21001 agggaatttc tgcacgggga tcaggcttcc ggcttcaggc tgatgaccgg cctgctggat
    21061 gaatacaagc ttgccaagtg gccattgtta actgtatgtc ccgtatacta caggccggat
    21121 gtggaggtct tcatcaaacc gactacagtt aaaggcgtaa ttgaatattt tgaactggaa
    21181 gggttcaaat acagcccgaa acccacctat gatttctatc gggcttaccg ggagcagatc
    21241 aaccggataa aaaaagaggt tgatgcctct ctgcaaattg acaatacagc tttctgcggc
    21301 tttcttatga tgtcgctgga aattccctcc taccgttgaa atggtgtatc gcttacggca
    21361 tttttagccg cgtttctttt cggcagcctc agcatctgcc ttggttttat ggactgtacc
    21421 ttttggatgc tggggaggtg cataaatgga gtatagctta ataggtatat ttcctgtatt
    21481 gattagattg tgccatgtgc ctgccggtat aataaaggca aagtcatcat agacctttct
    21541 ttgaaagttc agtctgtctt ttctttcacc catttttacg actccctgcc cttcttcaat
    21601 gcgtatgaat tggtcaagat cgggatgcat ttccaggcct atgtcgcctc caaccttgat
    21661 actcatcagt gtaagctgca aatgtcttcc tgtccataaa gcggtgcgga agttattatt
    21721 ctgtttggat gcctcctcga tatttaccac aaagggttcc ggcccatagt cctgtaattc
    21781 catagaatta tattcatctg cttcgcagcc catcatgggg cagggcatat gatacgtcgg
    21841 caagccgggg tagtatgggc atgggtactt tttatacaca ctaatcattc ctttcataga
    21901 ctaatactac catcatatgc caattactta aaaatgtgcc cggtttcaca tgctattagt
    21961 tccgtatcct cttatgaaaa agccatattc atcatacggg caacggcctg ttcgccttgc
    22021 tccttctgct tcaaatacgc aacccctgct cttcagtgaa aagttccctt atgtgacaag
    22081 atgagccaga ctaatttttt gacacctcac accattatat tgctgattcg ttactataca
    22141 atcaaaatca gacaaattct tgatataata aagttacatt taagacttaa aagcactgat
    22201 atgagtagga ctgatataca tattgcaatt gtttaataaa tatttcagaa agagaggatt
    22261 attgaaatgg agaaagcaac gaaaacactt tttttagtcg tgttgttagg ctgtatattt
    22321 gcacttaaca catatgcggc agaacctgaa gaaatatata ctgcggaaca actgaatgat
    22381 gtcagaaaca atctggctgg cagctacata ttgatgtcag atatagattt aagcgatact
    22441 gtatggggag aagtatatgc cggggaatac ggaaccagtg gttgggagcc tatcgggtca
    22501 caagcttctc cctttagagg cagctttgat ggtaatggtt atacaatcac cggacttttc
    22561 attgacaaac ccaatgtcaa tgaagtaggg ttgtttggca atactgcagc atcagctacc
    22621 attgaaggct tgaggctggg agatgtaaat gttaccggca agatggatgt tggaggttta
    22681 gtcgggctta atgagggtat aatcactaac tgctatgtga caggttcagt gaaaggtaca
    22741 gctgaagaaa ttggcggttt ggtgggttat cataagaacg caggcacaat cacaaattcc
    22801 tattctgcat gtgcagtaga gggaactcat tttctcggcg gactgatagg acgaaatgaa
    22861 gcacctatca gcaattctta tgccacagga gatgtgtatg gcatattggc gggcactagt
    22921 agtgctgttg gaggtctcgt aggtcgtaat gccgatggta ttattaattc ttatgctacg
    22981 ggaaatgtga ccggcggttc caacgtcggt ggtttaatag ggtacaactg gagtgatatg
    23041 aatgttgaat actcctatgc aacaggcgct gtagagggaa ccactaaagt aggtggtctg
    23101 attgggagta ttaacgttaa tggaactggc actgtgctaa actcctacta taattcttgt
    23161 acaaccggtc agacagaaga tgattccggc aatggtgagc caaagacctc aacggagatg
    23221 gtacaaacag atacttttat tgattgggat tttacaaaca cttggaacat taatgaatgg
    23281 gaatcctatc cgcgcttgaa gtggagcagt catgtgcctg cacctcctgc tgcaacagca
    23341 gtaacggtta gttctgcttc ggtggaattg acttatggcg gcgggccgac tacgttgaca
    23401 gcagccgtgg aaccggtatg tgcaatccaa aaagttacct ggtctacaag caactctgat
    23461 gttgcgacag tggacgaaaa tggtgtggta actcctatag gagcaggaga ggctactata
    23521 acagctactc cggtttccgg tgatgttgtt ggagaaactg ccgttactgt tggaaaaaag
    23581 gaattgactg tcagcgggac ctttaccgct ggggataaag tatatgacgg cagcacaaca
    23641 gcagtaattg atgcttcaag tctgaccctg tcaggtgttg tcggtggaga taacgttgaa
    23701 ctgagtacat cagcagtttt cgccaataaa aatgtggcag agaatattac ggttaatctg
    23761 accagcgaca attcgttgat tggtgatgat gccggcaatt atacattgtc cctcaccggt
    23821 gctccgattt caacagcaaa cataacagcc atggaattga cggtaatcaa tgcagcagca
    23881 caggacaagg tatatgacgg aactgctgat gctatcatcg acggagccca gcttagcggt
    23941 gtagtagacg gtgatagtgt aacattggaa gatcatactg tcggtatctt tgcacaagct
    24001 acggttggaa caggcattgg agtaaccaca aacatgacta taaccggtga tgatgccgat
    24061 aactatacaa ttgctcagcc ggggctttta actgcaaaca tcacagccaa ggaattgacg
    24121 gtaattaatg ctgtagcaca ggaaaaggta tatgacagaa ctactgatgc tgatattata
    24181 aatgcagagc ttagcggtgt agtagacggt gatagtgtaa cattggaaaa tcatactgtc
    24241 ggtacctttg ctcaagctgc aatcggaaca ggcattggag taaccacaaa catgactata
    24301 accggtgatg atgtcgaaaa ctatacaatt gctcagccgg tact
//
LOCUS       DAOQUR010000033        24285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2430_length_24285_cov_10.462031, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000033 DAOQUR010000000
VERSION     DAOQUR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24285
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_24285_cov_10.462031"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..488
                     /locus_tag="PKI50_08065"
     CDS             225..488
                     /locus_tag="PKI50_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03691.1"
                     /translation="MKMLRKPVEAIVLFDLEGNPVPVRFRYLDDSSELITVKVDRVIK
                     RELDKFAGNRMLKFTCQTRIGNQIKPFELRFEIDTSKWYLYKI"
     gene            complement(496..1743)
                     /locus_tag="PKI50_08070"
     CDS             complement(496..1743)
                     /locus_tag="PKI50_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNT03692.1"
                     /translation="MRTIMHIDVNSAFLSWQAVYNLQKGDTLDLREIPSVVGGSQADR
                     RGIVLAKSIPAKKFGITTGEVLWQARQKCPGLVVVPPDYNLYMKCSNILYETLKEYSP
                     GLQRFSIDELFLDYTGMEPHFGDSVAAAHMIKERVKKELGFTVNIGIGANKLTAKVAG
                     DLKKPDMVHTLFPDEIESKMWPLPVGDLFMVGRQTRKKLVEMNITTIGDLARADRRFL
                     RDRLKSFGNLIWCYANGIDESPVYPGYYLQMKGIGNSTTIKFDVTERQIAHKVLLSLT
                     ESVSLRLRAARACCRVVSVEIRTAELNFYSHQRKLMAHTNVTMDIFETVRELFDECWG
                     GEKIRHLGVRVTDLCSDEFMQASIFDQQCKYKKQALDSAMDVIRGRYGNDSVMRAIFA
                     DGEFAPMSGGSGGEDYPVMSSIL"
     gene            complement(1881..2393)
                     /locus_tag="PKI50_08075"
     CDS             complement(1881..2393)
                     /locus_tag="PKI50_08075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNT03693.1"
                     /translation="MKNMIMEMGGLMFCLTGSEYLDLILDIEKANSDFVFVWPKERHM
                     KAMNSEDEMHISVFSSAERKLVGYILLSGIGGEDSAVEFRRMVISDKGKGYGRTSVKF
                     IKKYCFEVLKCHRLWLDVFTDNEKAIGLYRSEGFVREGILRDCKKHGDSYRSMVIMSM
                     LEQEYIPCSF"
     gene            complement(2612..4282)
                     /gene="gltX"
                     /locus_tag="PKI50_08080"
     CDS             complement(2612..4282)
                     /gene="gltX"
                     /locus_tag="PKI50_08080"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNT03694.1"
                     /translation="MHNLTQNRELADLLFPDIQKTPEYYEMLYPKRQLPEGAKVTRFA
                     PSPTGYVHMGSLYASLVSERLAHQSNGVFFLRVEDTDKKREVEGGVSNIIRALSRFGI
                     SFDEGVTDVDDEKGSYGPYKQSERMEIYKTFAKRLVEKGLAYPCFCSEEDLDKIRAQQ
                     EEKKETPGYYGEYAVHRNSTLEQVKEELSRGKPFVLRVKAPDNPDSRFVFKDLIKGDV
                     EMPENFQDIVLLKSDGLPTYHFAHVVDDHLMRTTHVTRGDEWLPSAPLHVQLFEMLEW
                     EKPYFAHISPIMKMEGSSKRKLSKRKDPEADVSFYHEQGFPGISVIEYLANLINSNFE
                     DWRRENPESPNTSFKIELERMSVSGAMFDIVKLTDMSKDIVAGMTAEEVYNMSLEWAE
                     EYDSELWALLSRDEKYAKRIFSIDRGGEKPRKDIAKWLDVKPNIFYFFDELFTRDAAE
                     GYNFPDTIPADEVNRLIREYAKVYNHSDSKDMWFDRVKGFCEAMGYSKDVKTFKKNPE
                     AYKGHVGDVTGIIRVAVTNRRNTPDLYEIMQVMGEKMVMERFDKAINI"
     gene            complement(4316..5629)
                     /locus_tag="PKI50_08085"
     CDS             complement(4316..5629)
                     /locus_tag="PKI50_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNT03695.1"
                     /translation="MDILLKNAQIITLNDNDDVIPEGSIGIRDNRIDFIGEGNGCIEA
                     EYTKVIDCKGRTVMPGFVNAHNHLAMTMFRNYADDMKLMDWLFTKIFPLEDKQNDESV
                     YWGSLLAMVEMIKSGTTTFADMYFFMESTARAAAESGMRAVLSRGLQGESGEEEPDYR
                     LKESDELFRKFHNSYNGRIKVMLAPHSVYTCSEAYLRKVAAKSLEQGVPVQIHLSETK
                     EEVSTCIEKFGRSPVKYLEDLGLLNDRTVAAHCVAVDDDDIGILASRRVNVVHNPGSN
                     MKLASGIAPVAKMLEKGINVCLGTDGASSNNNLDMLEEMRLAAYLQKIYTNDPTALPA
                     EAVIRMATLKGAKALGFENLGSIEAGKAADLIVLNTERAHYYPKHNIKSAIVYSGNSA
                     DVETVIIDGNLVMESGHLVTLDEERILYKAQKWASKLTGQECPQP"
     gene            complement(5633..6883)
                     /locus_tag="PKI50_08090"
     CDS             complement(5633..6883)
                     /locus_tag="PKI50_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNT03696.1"
                     /translation="MRESIIRDISSAESGHRKIEWVKGHMPVLSAIEKEFIEEQPFAG
                     KKIAMSIHLEAKTAYLSKVLKAGGAEVSVTGSNPLSTQDDVAAALAEDGLNVYAWYNA
                     TPEEYNHHLNAALDIGPNIVIDDGGDLVHLLHGERQELLPDILGGSEETTTGVLRLKA
                     REREGLLKFPMISVNDAYCKYLFDNRYGTGQSVWDGIMRTTNLIVAGKNAVVIGYGWC
                     GKGVAMKAKGLGANVTVCEIDPIKAIEAYMDGFQVMSVDEAASIGDFFITVTGCAKVI
                     TEKHYKAMKNGVVLANAGHFDVEIWKPDLETLSCGKKPMRNNIMGYVMEDGRIINLLA
                     EGRLVNLAAGDGHPAEIMDMSFALQALTARYISLNKEKLENRVYKVPEEIDRKVAYMK
                     LKALGIRIDKLTKEQEEYLRGWGE"
     gene            complement(6948..9359)
                     /locus_tag="PKI50_08095"
     CDS             complement(6948..9359)
                     /locus_tag="PKI50_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03697.1"
                     /translation="MSRFRELTPEELSNKIDCGLFEFETTADLDPLNGIIGQSRAAAA
                     MEFGLAIKRPGYNIYVSGITGTGRSSYTRSIVSRIAANESIPNDWCYVYNFKNPDKPK
                     CINLPAGMGYKLQKDMKRLLKDLKIKVPQAFEGEEYEKQKGQIIQEYQAKSSEYMESF
                     NVFAREQGFVIRKSEHGLMTIPLRDGKPMDDKDYIEMPPEERKKIEDNSFMVQGKLME
                     TLKRMKEIEKAARDKIDQLETKIALLAVEQPIMELKEKYDKYKNIIEYLQAVQRDIIH
                     NVEDFRNLEVGKAETLDLVERIREKDFTIKYQVNLAVDNRDTKGAPVILESNPKYDNL
                     LGKIEYESNMGVVTTDFTKIKAGSLHQANGGYIIINMSDLMRNPESWEGIKRSIKTGN
                     ITIENIFSMSGIVSGGIKPEPIPLNVKIILIGSSYVYQILYTHDEDFRKLFKIKADFD
                     TDMSSDKGNCLKYASFIRSHCEENGLMHFDRSAVAAVIEYSRRLAEHKYKLSTRFNDI
                     IEVLYEADAWARIAGEQKVTGSSVKRAIEQKWYRSSRIEEKMQELIDTGVVLISTEEK
                     VVGQVNGLSVMNFGDYTFGKPSRITAAAYAGEKGIVNIEREVEMSGSIHDKGVLILTG
                     YLGDKFAQDFPLTISASICFEQLYEGVEGDSASSTELYALLSSLSELPVKQYIAVTGS
                     VNQKGEIQPIGGVNEKIEGFFQTCKTRGLKGNEGVMIPWQNVDNLMLKDEVIEAVREG
                     MFHIYPVKKIEEGIEILTDVPAGTRDESGNYPENTVFHLVQKKIEKFARIAEEFDDCD
                     QERRV"
     gene            complement(9398..>9582)
                     /locus_tag="PKI50_08100"
     CDS             complement(9398..>9582)
                     /locus_tag="PKI50_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNT03698.1"
                     /translation="AMGVGGSLDVLAGKVKRAPEFYQKAGLEWFYRLVKEPRRITRVL
                     RIPKFIALAFYDAKVK"
     assembly_gap    9583..9682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9931..11190
                     /locus_tag="PKI50_08105"
     CDS             9931..11190
                     /locus_tag="PKI50_08105"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT03699.1"
                     /translation="MEKILIEGSQKLTGEVSISGAKNAAVAILPAAILAGSACTIKNL
                     PMVKDVTYLRDMLIQMGGKIHILDNDKIKIDTSGIVTHKIDLELAKKMRASYYFLGAL
                     LGRFKRAEVPFPGGCDIGVRPVDLHIKGLEALGAKIKISHGLIKAKTDELKGARIYLD
                     VVSVGATINIMLAACMAKGTTTIENAAKEPYIVDTANFLNALGANIKGAGTDVIKIKG
                     VESLGGCEYSVIPDQMEAGTYMIATAATRGDVLIKNIIPTHLDAVSAKLREMGVEITE
                     YEDSVRVKAPSRTKAVNVKTMPYPGFPTDMQQPMSVLLSVSEGTGIVTESIFESRFKY
                     FDELKRMGAKIKIEGRVAVIEGVSKLTGAPVYATDLRAGAAMVVAGLTAEGKTTISNI
                     SYIDRGYERLEEKLINLGAKIRRIEEK"
     gene            11202..>12307
                     /locus_tag="PKI50_08110"
     CDS             11202..>12307
                     /locus_tag="PKI50_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNT03700.1"
                     /translation="MKLKQSLSILLVFCFFIVILMPGTAAAAASGTKIPGAFWSLVTP
                     YQNAVDANDLNNIIVYGNKIIELFNGMEETQQVLEIITPRLDQVAKAYEALGRFDLAV
                     EYFSKYIPRAEKLGWKDGVTYARSKINTLGFDIELYAKTTNLEKSVYFGSKYEPRSGV
                     YFGSNYDTDPRIGTYSWNDIKNYFPRKNTAYLTYLHWNEDIKSFDRYFKDAKANNIAV
                     QLAWNIEDSEIDNVLKSIKSYDSYIRATADYLGKLNIPVFLRFAGEMNIKDNCKDSNS
                     YIAAFRHVSGIMKAKAPNVAMVWSPNDISAKGRTYDQYYPGDEYVDWIGISTYTQRYF
                     QGKKDWGNLQDTNDSVYMTGNYANPLAKIKPIMDA"
     assembly_gap    12308..12407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12408..13505
                     /locus_tag="PKI50_08115"
     CDS             <12408..13505
                     /locus_tag="PKI50_08115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT03701.1"
                     /translation="MRLDIGGAETHIVELAKGLSREGFNVIAASAGGVYEKELAESGI
                     KVYHVPLDNKKPHNVIKAYFKLKNIIKENRIDLVHAHARIPGFICGLLHKRMGFPFVT
                     TAHGTWKTGYGLRYITNWGQKTVAVSEDIRQYLLDNYKIEDKDIKVTINGIDTDKFSS
                     GIDCSDIMDELNLKPEDMKTVYISRFDSDRSYIINKLLEVIPELSESIPSLKVIMVGS
                     GNILDSIKEKAREINNITGQNTIIVTGSRTDINKFTALADLFIGFGRSSLEALSAGKP
                     LILAGNEGYIGILNENSLDDAIKSNFSGRGKSKADSKSFKDDILKVLSLSGEERKALG
                     DFGRSVIENSYSVRKMVQDNMEVYAQLLEHK"
     gene            13519..14610
                     /gene="csaB"
                     /locus_tag="PKI50_08120"
     CDS             13519..14610
                     /gene="csaB"
                     /locus_tag="PKI50_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HNT03702.1"
                     /translation="MYDVMISGYYGFGNSGDDAILLAIIENLRKIKKDIRIVVLSKNP
                     KDTASIYGVDSIDRFNLFKVIGTMKKTRLFLNGGGNLIQDVTSTRSLVYYLTTIYLAK
                     LMGLKVMLYANGIGPVGRRTNRYFTSKIIDRVDIITLREEASGLELKSLGVKKPEIRV
                     TADPALGLEPAGSEETEKLLQKEGIPADTPLIGFSVRKWSGYEAYSRILAQAADYIEE
                     TYNAKSVFIPLHFPSDLAAAEDIASKMKHAPFILRNIYETDKILGIMKKMDLVLGMRL
                     HALIYSVSLSVPVIGLIYDPKVQGFLEYVNQPWAGNVADLNLDSLKKLIDEVWSSRKE
                     ISQQLEKGNRLLKQKAYENAEIAIKLLEQ"
     gene            complement(14634..15140)
                     /locus_tag="PKI50_08125"
     CDS             complement(14634..15140)
                     /locus_tag="PKI50_08125"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HNT03703.1"
                     /translation="MLLDEKGRLFGKISIVDIGVLLLIAVLIGGVYYKFFMVDKDQNA
                     AKFDTIEYKVLVEEVRQQSVDAIEIGADIYDVKTNSPMGSITDIEVLPATDQLTKADG
                     TMVIAEKPERFNVLITIRVPGIETKYGFRANGRLDINRESQQVMDTRMIEMVAKIVDV
                     KNLSKAQQ"
     gene            complement(15153..15659)
                     /locus_tag="PKI50_08130"
     CDS             complement(15153..15659)
                     /locus_tag="PKI50_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HNT03704.1"
                     /translation="MKDMALRDDRGRLFGKINIMDLIIILLIILTAAGGYMFFSGGNS
                     RQAVNTGKVVYDFEITNVNKDFVDAITPGDPIRDSTRGNELGTVVSKSSRDAAMLNED
                     LINGRYIIAEVPDAYDVVITIEADANITPASIIIGGAEVKVGKKFFIKGKGYANQGFV
                     TKMTLVGQ"
     gene            complement(15661..16770)
                     /locus_tag="PKI50_08135"
     CDS             complement(15661..16770)
                     /locus_tag="PKI50_08135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT03705.1"
                     /translation="MIKVMHVLSDMKIGGAGSWLLNLLGAIDKEKFEIKVVLPRGSML
                     TDKIKNLGFEVIAIDGIKDKSYDARAVNLLYYIFRAEKPDIVHTHASLSARLAGKRAG
                     ARIINTKHCIDSPITGIRRFAGALINDRYSDGIIAVSEAVKRNIAENGVPDDKISVIY
                     GGINPVKVLDDKEKSLARGKWGIGEEEVVVGIVARLAEVKGHKYFMDAAEIISRDNDN
                     VRFFIAGIGPKEEELKELIKQKGLTDKVVFTGFVDEIYEIFNIIDINVISSLSEALCL
                     SLIEGMSVGKPSVAADTGGIPEVVKDGYNGFLVPPGDAERLADAILKLIHDPELRKTM
                     GDRGREIMEKSFTAEIMAEKIEELYESVARKNRRE"
     gene            complement(16767..18683)
                     /locus_tag="PKI50_08140"
     CDS             complement(16767..18683)
                     /locus_tag="PKI50_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT03706.1"
                     /translation="MKESLIFGMILKVYDRIVFYYENSMFFIVMQRLGHGFGKLAASS
                     SILNFFDREWNIADTWRQSLVFRILVSPLRWFRFASQKFSESVNVAIEESRVLKAIRV
                     FPENLFDMSTRVYGLLFITFSAVFGLLDFTSRNAGMLFGMSEIMKLALFLGGAVLILI
                     NRPVKALAEGSIVSKAVFGFFNVSHDAFYDKRTEDAVLNKRFEITAIVTGTALGAMWH
                     FLQPMAFALAVGGIAALVLVLWRYEIGVFAVAGFIPLAPTMALLGLILLTAVSFFIRL
                     FRDKSMKLRSTILDYFVILFGMVLLYSFITSYTPGSSMLALLIHMAFIVFYFILVSTI
                     KTRRQLYFLAALLVLSTTIASLYGLYQLKTVGATSEAWVDTTLFEDIKARVGSTFENP
                     NVLGEYLVLIIPIAIAMLWGQKNWLSKLAILGLTAIMLGCLVYTYSRGAYVGLMLAFA
                     LFAVLRDRRFVILGVVGLLMLPFVLPASVINRFASIGNLNDTSSFYRISVWLGSLKLA
                     QDYWLSGIGLGLEPFKLVYPKYSLNAAYAHHSHNIYIQLMIETGIAGLLMFFAIIAVY
                     YKTLLAGFYKTKDKFISTFMIAVASGMAGYLAQGMVENIWYNNRVLLTFWVMLAFGVI
                     TKALITEDNEVSGI"
     gene            complement(18917..19465)
                     /locus_tag="PKI50_08145"
     CDS             complement(18917..19465)
                     /locus_tag="PKI50_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03707.1"
                     /translation="MVNSKSRKTSIIVLTAIILVLSAVVISAADGVTPGSEQDPIVTQ
                     SYVEQKSEQIKYYIDSLIAQETAKLKTELELKEQEIAKLREEIKDAAKAAGKFQVVEM
                     QKGQMLIAGEGAEIIPRSGKFSAIEDAFGGLSDITAAKDLKNGEAVVNNHMLIAARGE
                     GRGLKALADKSFLIIKGTYSLQ"
     gene            complement(19747..20910)
                     /locus_tag="PKI50_08150"
     CDS             complement(19747..20910)
                     /locus_tag="PKI50_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNT03708.1"
                     /translation="MSKEKILKTYARFDVTAVSGKGCLIYDINGKEYLDFVSGVAVNC
                     LGHCHPAIINAIREQSCKLMHVSNLYWNELQMELADRLCELSGLEGAFFCNSGTEAIE
                     AAIKFARKYGKLKGGALKHEIIYLNNSFHGRTLGALSVTGQEKYQKDYMPLIPGVKSV
                     DLNAAGQLRAEMNPKVCAIIAEPVQGEGGILAADKSFLEEAKKLCEKYDSLLIFDEVQ
                     CGIGRLGSLFAFEKFGVKPDLVCIAKGLGGGFPIGAVVASGKAAECIAYGDHGSTFGG
                     NPLACKVALTILEELLQNGVLENVEHMSKYFVGKLQALKEKHGSIKSIRGIGLLLGLQ
                     LSIDTKKFIDECIDSGLLLVGAGADVVRLLPPLNIQEEYIDRAIDIIDSVLSK"
     gene            complement(20903..21730)
                     /gene="argB"
                     /locus_tag="PKI50_08155"
     CDS             complement(20903..21730)
                     /gene="argB"
                     /locus_tag="PKI50_08155"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNT03709.1"
                     /translation="MDLGGTVVIKYGGSITKDKEATDAFLEDAAILRNAGVKVVIVHG
                     GGPDINRMLGRLNIAGEFVKGLRKTNEAAMEVVEMVLSGRVNKELTSKLIGKGADAVG
                     ISGRDGKLLEAERMYLNDNGSNIDIGYVGEVTKVNGKILTDLLSLGYVPVISPVAEGK
                     EGKAYNINADYAAAAIGASIGADSLVYLSDVDGLFGDIGNPDSLISSITTAEIEKLVQ
                     AGAISGGMIPKMKCCIEAIEKGVKSVCLIGGKKPHILLELFHGKEAGTIIIGGEENV"
     gene            21880..22917
                     /gene="argC"
                     /locus_tag="PKI50_08160"
     CDS             21880..22917
                     /gene="argC"
                     /locus_tag="PKI50_08160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNT03710.1"
                     /translation="MINAGVVGSTGYAGYQLSMILSQHKNVSIVFLSSHNYSNLRFSD
                     VYGNLKYLLDNMCIDMQDIESKLSKIDVLFLALPHGQSFDLVEKALGLGVKVIDIGAD
                     YRLRDQKVYETWYGVTHKLPGRLKDAVYGLPELYRDSISNSSLVANPGCYPTASILAL
                     APLVHAGIIDNTSIIIDAKSGVSGAGRSANVSSLFCECGESVKAYNIATHRHTPEIEQ
                     QLSDAAGAKITLSFTPHLVPMSRGILATCYGSLKKDISQSELYDLYRSYYKNENFIRI
                     TDSLPETRFVKGSNICDIAVRVDERTNRVIAISAIDNLIKGAAGQAVQNMNILFGLYE
                     HTGLDMLPNML"
     gene            22932..24149
                     /gene="argJ"
                     /locus_tag="PKI50_08165"
     CDS             22932..24149
                     /gene="argJ"
                     /locus_tag="PKI50_08165"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNT03711.1"
                     /translation="MKIIKDMTITDVPYFKASGVSCGIKKNGNKDLCVIYSEKDSVAA
                     AVFTTNKVKAAPVILDMKHIQSKSIRAIVANSGNANACTGKQGQKDANTMAELTASLL
                     NIPSDSVLVASTGVIGVPLPMKNIEAGIKNACDNLSYDGGKDALRAIMTTDTCEKSVT
                     VEVELEGKKVLLSGIAKGSGMIHPNMATMLGFITTDANISKEMLQEALSESVKKTYNM
                     ISVDGDTSTNDMVIMLANGAADNKLIGARDKNFDAFMKALDLLNTELAKKIARDGEGA
                     TKLIECTVSNAVSYADACILSKSVIGSNLTKAAFFGNDANWGRILCALGYSGGNFSPD
                     KADIYFESEAGCIQLVADGEPLQFCEETASAIMDKEYVIIKVDLKEGGYSATAWGCDL
                     TYDYVKINGSYRS"
BASE COUNT         6443 a         6061 c         4743 g         6838 t
ORIGIN      
        1 cagcgaagtt tctgaacagg caaaaacatg ctaatattaa agcacataag aagaagaatg
       61 cgacggggac acggggacgt ggggacacgg ggacgtttcc cctgtcccac tgaaagcggg
      121 acaggggaaa cgtccccatg tccccgaaac gtccccatgt ccccgtcgca ttcttcttct
      181 attctgcaca acaagaaaat tattccatat aggaggtgaa ctaaatgaag atgctcagaa
      241 agcctgttga ggcaatagtg ctttttgatc tggaaggcaa tcctgtacct gtaaggttca
      301 ggtatcttga tgacagttcg gaattgatca cggtaaaagt agaccgggta ataaaaagag
      361 agcttgacaa gtttgccggc aacaggatgc tcaagtttac ctgccagaca cgcatcggca
      421 atcagatcaa gcccttcgaa ctcagatttg aaatagatac ttcaaaatgg tatttatata
      481 agatttaact gtctgtcaca gtatgctgct cattacggga tagtcttctc cccctgaccc
      541 tccggacatg ggggcaaact ccccgtcggc aaatatggcc ctcattacgg aatcattccc
      601 atacctgcct cttataacat ccatggcact gtccagcgcc tgcttcttgt atttgcactg
      661 ctggtcaaat attgaggcct gcatgaactc gtcgctgcac aaatccgtca ctcttacccc
      721 cagatgcctt attttttcac ctccccagca ttcgtcgaac agttctctta cagtctcgaa
      781 tatatccatc gttacattgg tgtgagccat aagctttctt tgatgggaat aaaaattaag
      841 ctccgctgtc cttatctcca cagataccac ccggcagcag gctctggcag ccctgagcct
      901 taatgaaaca gattccgtca gggacaataa taccttgtgg gcaatctgcc tttcagttac
      961 atcaaacttt atagtggtgc tgttgcctat tcctttcatc tgcaggtaat atcccgggta
     1021 taccggcgat tcatcaatac cgttcgcata acaccatatc agattaccga aggatttaag
     1081 cctgtctctc aagaatcttc tgtctgccct ggccaagtct cctattgtgg ttatattcat
     1141 ttccacaagc ttctttctgg tctgcctgcc taccataaat aaatcgccta caggcagcgg
     1201 ccacattttg ctctctatct cgtcaggaaa caaagtatgg accatatcgg gcttttttag
     1261 atctcctgca acctttgcag ttaacttatt tgcccctatc cctatattga cggtgaagcc
     1321 caattccttt ttcaccctct cctttatcat atgtgcggcg gcaactgaat ctccgaagtg
     1381 gggttccatt cccgtataat ccaaaaacaa ttcgtctatg gaaaatctct gcaaaccggg
     1441 agaatactcc ttcagagttt catacagtat attgctgcat ttcatgtaaa ggttatagtc
     1501 agggggcacc actacaagcc cggggcactt ctgcctggcc tgccacagca cctccccggt
     1561 tgttattcca aactttttgg caggtatgct tttcgccagc actatacctc tccggtcagc
     1621 ctgggagcct cccactaccg aaggtatttc cctaaggtca agggtatccc ccttctgcaa
     1681 attgtaaaca gcctgccagg ataaaaaagc cgaattcaca tcaatatgca ttatggttct
     1741 catgccgact ccgccttccc tgcaatcaaa aatcctcata tattatgcga acgtatgttc
     1801 gatttaatta tatcatcttt tggcatggaa gggaagtatt tctgagtccg gtcctattta
     1861 aaagtttaat gggaacaagt tcagaaggaa caggggatgt attcctgctc cagcatagac
     1921 attatcacca tagagcggta gctgtcccca tgcttcttgc aatccctgag tattccttcc
     1981 cgtacaaaac cttcacttct gtaaaggcct atcgcttttt cgttgtcggt aaacacatcc
     2041 agccacagcc tatggcattt caacacctca aagcagtatt tttttataaa cttcacgctt
     2101 gtccgtccat agccctttcc cttgtcggat atcaccatgc gtctgaactc tacggcactg
     2161 tcctctcccc cgatccctga aagcaatatg taacccacca gcttccgttc ggcagagcta
     2221 aatacggata tatgcatttc atcctcactg ttcatggctt tcatgtgcct ttccttcggc
     2281 catacaaaca caaaatcgct gttagccttc tcaatatcaa gtatcaggtc gagatattcc
     2341 gaaccggtaa ggcaaaacat aagcccgccc atttccataa tcatattttt cataacccgc
     2401 ccccctcagg tttattttat aatatggtaa tataatatac aatatttaga agggattgct
     2461 gcacaaaata tgaatgatgc ggcatgggga agtttctccc ggggacacgg ggacgtttct
     2521 ccgaggacat ggggacgttt ctcctgtccc gctgaaagcg ggacaggaga aacgtcccca
     2581 tgtcctcgcg tccccatgtc ctcacatata tttatatgtt aatagccttg tcaaatctct
     2641 ccattaccat tttttcaccc ataacctgca tgatctcata aaggtcggga gtattcctcc
     2701 tgttggtaac agctaccctg ataattcccg taacatcacc cacatggcct ttataggcct
     2761 ccggattttt cttgaaggtc ttcacatcct tggagtaccc cattgcctcg cagaatccct
     2821 ttactctgtc aaaccacata tccttgctgt cggaatggtt ataaaccttt gcatattccc
     2881 ttataagcct gttaacctca tcggcaggta ttgtgtcagg aaaattatat ccctctgccg
     2941 catcccgggt aaacagctca tcaaagaaat agaatatatt cggtttcaca tccagccatt
     3001 ttgcgatgtc ttttctgggc ttttcgccgc ctctgtctat actgaaaatc ctttttgcat
     3061 acttctcatc tcttgacaga agggcccaaa gctcagaatc atattcctca gcccattcca
     3121 gggacatatt atagacctct tctgcagtca tcccggcaac aatatccttg ctcatatccg
     3181 taagcttgac tatatcaaac atcgcaccgc ttacactcat tctttccagc tcaattttga
     3241 agctggtatt gggggactcg gggttttccc ttctccaatc ctcaaaatta gaatttatca
     3301 ggttcgcaag gtattcaatt acggaaatgc ctgggaagcc ctgctcatga taaaagctta
     3361 catccgcctc cgggtccttt ctcttactga gctttctttt agaggaacct tccatcttca
     3421 ttatcgggga gatatgcgca aaatacggct tttcccattc aagcatttca aaaagctgca
     3481 catgcagagg agccgaaggc agccattcat cgccccttgt tacatgagtg gtcctcatca
     3541 ggtggtcgtc aaccacatgg gcgaaatgat aagtgggaag cccgtcggac ttcagaagta
     3601 caatatcctg gaaattttcc ggcatttcca catcgccctt tatgagatcc ttgaaaacaa
     3661 acctgctgtc aggattgtcg ggtgctttta cacgcagtac gaagggcttg ccccgggaaa
     3721 gctcttcctt tacctgctcc agagtggaat tcctatgcac ggcgtattca ccgtaataac
     3781 cgggggtttc cttcttctct tcctgttggg ctcttatctt atccaagtct tcctcagagc
     3841 agaagcaggg atatgcgagc cctttttcca ccaaacgttt tgcaaaagtc ttgtatattt
     3901 ccattctttc actttgctta tatgggccgt agctgccctt ctcatcgtct acgtctgtta
     3961 ctccttcatc aaagctgatg ccgaacctgg agagggctct tataatattg gaaactccgc
     4021 cttccacctc acgcttttta tccgtatcct caactctcag gaaaaatact ccgttgctct
     4081 gatgggcaag cctctccgat acaagagatg catataagct tcccatgtgc acgtagcctg
     4141 tagggctggg ggcaaacctg gttacctttg ctccttcagg cagctgcctt tttggatata
     4201 acatctcgta gtattccggt gtcttttgga tatcaggaaa tagcaaatcc gccaattccc
     4261 tgttttgtgt caaattatgc attataacaa accgcctttc gttagtagtg tttccctaag
     4321 gctgcgggca ttcttgccct gtcagctttg aggcccattt ctgagcttta taaagtatcc
     4381 tttcctcatc aagagtgaca aggtgtccgc tttccattac caaatttccg tcaattataa
     4441 ccgtctccac gtcagcggag ttcccggaat aaacgattgc ggattttata ttatgcttcg
     4501 gataatagtg ggctctctcg gtattaagaa ctatcaggtc tgcagccttt cccgcctcga
     4561 tgctgccaag gttttcaaat cccaaggcct ttgcaccctt caaagttgcc attctaataa
     4621 cggcctccgc cggcagagcc gtaggatcat tagtatatat tttctgcaaa tacgctgcca
     4681 gcctcatttc ttccaacatg tccaggttat tgttgctgga agctccgtca gttcccaggc
     4741 atacatttat gcctttctcc agcatctttg cgaccggcgc tattccgctt gccagcttca
     4801 tattgcttcc cggattatgt accacattta ccctgcggga agctaaaatt ccaatgtcgt
     4861 cgtcatcaac tgccacgcag tgtgccgcta ctgtcctgtc attcagaagc cccagatcct
     4921 caaggtactt caccggtgac cgtccgaatt tttcaatgca ggtactcacc tcttcctttg
     4981 tctccgaaag atgtatctgc acaggtaccc cttgttccag ggactttgcc gccaccttcc
     5041 tcaaatatgc ctccgaacag gtatacaccg aatggggggc cagcatcacc tttatccggc
     5101 cattatagct gttatgaaac ttccggaaca gctcatcact ttccttaagc ctgtaatccg
     5161 gctcttcttc ccctgattcc ccctgaagcc ccctggagag aacagccctc atcccggatt
     5221 cggcagccgc acgggcggtg ctttccataa agaagtacat atcggcaaag gttgttgtcc
     5281 cgctttttat catctcaacc atggcaagaa ggcttcccca atacaccgat tcatcattct
     5341 gcttgtcttc caggggaaat atttttgtaa aaagccaatc cataagtttc atatcatcgg
     5401 cataattcct gaacattgtc attgccagat ggttatgggc atttacaaaa ccgggcataa
     5461 ccgtcctgcc cttgcagtca ataacctttg tatactctgc ttcaatacac ccattgcctt
     5521 cccctatgaa gtcaatccga ttatcccgta ttccgatact gccttccggt attacgtcat
     5581 cattatcatt aagggttata atctgcgcgt tttttaacaa tatatccatg tactactctc
     5641 cccagcctct aagatattct tcctgctcct ttgtaagctt atctatcctt attcccagag
     5701 ccttaagctt catataggca acttttctgt ctatttcctc cggtacttta tataccctgt
     5761 tctcaagctt ttccttgtta aggcttatat atcttgcggt aagagcctgc agtgcaaagc
     5821 tcatatccat tatctcggca ggatgtccgt ctccggcggc aaggttcaca agccttccct
     5881 ccgccagcag gtttattatc cttccgtcct ccatgacata tcccattata ttgtttctca
     5941 ttggtttttt cccgcagctt agggtttcca aatcgggctt ccagatctca acgtcgaaat
     6001 ggccggcgtt ggccagtaca actccgttct tcatggcctt gtagtgcttt tctgtgatga
     6061 cctttgcgca gcctgtgaca gttataaaga aatctcctat ggatgccgct tcatctacgg
     6121 acatcacctg aaaaccgtcc atatacgcct ctatagcctt tatagggtca atttcacata
     6181 ccgttacatt ggcgccaagg cccttggctt tcatagccac acccttgccg caccatccat
     6241 agcctattac caccgcgttc tttccggcaa ctataagatt ggtggtcctc attattccgt
     6301 cccataccga ctggcctgta ccgtacctgt tgtcaaacaa gtatttgcag taagcatcat
     6361 tgacagaaat catgggaaat ttgagaaggc cctctctttc cctggcctta agcctcaata
     6421 ctcccgtagt tgtttcctcg ctgcctccca atatatcagg cagcagctcc tgcctttcgc
     6481 catgcagaag atgcaccagg tcgcctccgt catctatgac tatattgggg cctatatcca
     6541 aagccgcgtt cagatgatgg ttatactcct caggagtggc attataccag gcatatacat
     6601 taagcccgtc ctccgccagc gctgccgcaa catcgtcctg ggtggagagg gggttgcttc
     6661 ctgttacgga aacttctgcg ccgcccgctt tcaatacctt ggaaagatat gcagtctttg
     6721 cttcaaggtg aattgacata gctattttct tgcctgcaaa aggctgctct tctataaatt
     6781 ccttctctat agcagacagt acaggcatat ggccttttac ccattctatt ttcctatggc
     6841 ctgactcagc cgagctaata tctctgatta tactctctct cattcttaaa cctccctatc
     6901 tcgtgccccg tacctcgcac cccgtacccc gtacctcgtg cccgtatcta tactctgcgt
     6961 tcctgatcgc aatcgtcgaa ctcctcggca attctagcaa acttttctat ctttttctgt
     7021 accaaatgga atactgtatt ttcaggatag tttccgctct catcccttgt tcctgcaggt
     7081 acatcggtaa gaatttctat tccctcctct attttcttca caggatatat gtgaaacatg
     7141 ccttccctga cggcttctat gacctcatcc ttcaacataa gattatcaac attctgccag
     7201 ggtatcatta ctccttcatt ccctttaagg cctctggttt tacaggtctg gaaaaagcct
     7261 tctatctttt cattgacacc gcctatgggc tgtatctcac ctttctggtt aaccgatccg
     7321 gtaacagcta tatattgctt tacaggcagc tccgacaggc tcgacagcag ggcatacaat
     7381 tcggtgctgg atgcgctgtc cccctcaacg ccttcataaa gctgctcaaa gcatatgctt
     7441 gccgaaattg taaggggaaa atcctgcgca aacttgtctc ccaggtatcc ggtaagtatc
     7501 agcacccctt tatcatggat gcttccgctc atttcaactt cccgttcaat atttactatg
     7561 cctttctctc cggcataggc ggctgccgtt attctgctgg gcttcccaaa ggtgtaatcg
     7621 ccgaagttca ttaccgagag tccgttgacc tgccctacaa ccttctcttc cgtagaaata
     7681 agcactaccc ccgtatcaat cagctcctgc atcttttctt ctatcctgct tgaacggtac
     7741 cacttctgct caattgccct ttttacactg ctgcccgtta ctttctgttc tcctgctatt
     7801 cttgcccagg catcagcctc atataatact tcaattatat cgttgaatct tgtggaaagc
     7861 ttgtatttat gctccgccaa cctcctgctg tattcaatta cggctgccac ggcacttctg
     7921 tcaaagtgca taagcccgtt ttcctcacag tgagatctta taaagctggc atatttcaaa
     7981 cagtttccct tgtcactgct catgtctgtg tcaaaatccg ccttgatttt aaaaagcttt
     8041 ctgaagtcct catcatgggt gtaaagtatt tgatatacat aggagcttcc tataagaata
     8101 atctttacgt tcaatggtat gggctccggt tttatacctc cggatacaat acctgacatg
     8161 gaaaatatat tttcaatagt tatgtttcct gtttttatgg atctttttat tccttcccag
     8221 ctttcaggat ttctcatcag gtcggacatg ttgattatta tatagccccc attggcctga
     8281 tgaagcgacc ctgcctttat cttggtaaaa tctgttgtga ctacccccat attgctttca
     8341 tattcaattt tacccaatag attatcatat ttggggtttg attccaaaat gacgggagca
     8401 ccctttgtat ctctattatc tactgccagg ttaacctgat attttatggt aaaatccttc
     8461 tcccttattc tttccaccag gtcaagagtt tcagccttac cgacctccag gttcctgaaa
     8521 tcctctacat tatgtattat atccctctga acagcctgaa ggtattcaat tatattcttg
     8581 tacttatcgt acttttcctt cagctccatt atcggctgtt ccactgcaag aagcgctatt
     8641 tttgtttcca actggtcaat cttgtctctg gcggctttct ctatttcctt catccgcttc
     8701 agcgtctcca ttagttttcc ctgaaccatg aaggaattat cctctatctt ctttctttcc
     8761 tcaggaggca tctctatata gtctttgtcg tccattggct ttccgtcccg gaggggaatg
     8821 gtcataagcc cgtgctcgct ctttcttatt acgaagccct gctctcttgc aaaaacattg
     8881 aaggactcca tatattcgga gcttttcgcc tgatactcct gtataatctg acccttctgc
     8941 ttttcatatt cttccccttc aaaagcctgg ggaaccttta tcttaagatc ctttaagagc
     9001 cttttcatat ctttttgaag cttgtacccc attccggccg gcaggtttat acacttaggt
     9061 ttgtcaggat tcttgaaatt atatacatag caccaatcat taggtatgct ttcatttgcg
     9121 gctatcctgc ttactatgga cctggtatag ctgctccttc cggtgcctgt tattccggag
     9181 acatatatat tatagccggg ccttttgatt gcaagcccga actccattgc tgcagcagcc
     9241 ctgctctgtc cgataatacc gtttaacgga tccaggtccg ctgtggtttc aaactcaaat
     9301 aagccgcagt caattttgtt gctcagttcc tccggagtca gttctctaaa tctgctcatt
     9361 cagcactctc ctctcaactt taatacattc ctcatatcta ttttaccttt gcatcataga
     9421 atgcaagggc tatgaatttg gggatcctca ggactctagt aattctcctg ggctccttca
     9481 caagcctgta aaaccactcc aacccggctt tctgataaaa ctcgggagcc cttttcactt
     9541 tgcccgcaag cacatccaga cttcctccca cccccatggc aannnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nngcttcatc catatttaca ttatcaattt ttattcctac
     9721 tacttctacc tcgacatgca taagccatcc atccttaaat ccaatatata aagcctaatg
     9781 tatctggtaa atattatatc atattatcaa atcataaaaa cagtgatata atgtagatgc
     9841 aataagctat atgtcaaatt tttgccggag caggtttttg caggtgtgca aaaaccctca
     9901 ttaaaatctt gttataaggg gtcggatgta ttggaaaaaa tattgataga gggctcccag
     9961 aagcttaccg gtgaggtcag catcagcgga gctaaaaatg cagcagtggc aatactgcct
    10021 gctgcaattc ttgcaggctc agcctgcact ataaaaaacc ttcccatggt aaaggatgtt
    10081 acttatctga gggacatgct tatacaaatg ggagggaaaa tacatatact tgacaatgat
    10141 aaaataaaaa tagacacttc gggaatcgtc acccataaaa tagacctgga attagcaaaa
    10201 aaaatgaggg cttcgtatta tttcctgggt gcccttcttg gcaggttcaa aagggccgag
    10261 gttcccttcc cgggaggctg tgatatagga gtaaggcctg tggatcttca tattaagggc
    10321 cttgaggctc tcggggccaa aatcaaaatt tcccacggcc ttataaaagc aaaaaccgat
    10381 gaactgaagg gtgcaagaat atacctggat gtggtaagcg taggagccac tatcaacata
    10441 atgcttgccg cctgcatggc aaaaggcact actactatag aaaacgcggc aaaggagccg
    10501 tatatagtag ataccgccaa cttcctcaac gcgttgggag ccaatatcaa gggcgcaggc
    10561 actgatgtca ttaaaatcaa aggtgttgaa tcccttggag gctgcgaata cagcgtgata
    10621 cccgatcaga tggaagcagg aacctatatg atagcaactg ccgctaccag aggcgacgtt
    10681 cttatcaaaa atattatccc cactcacctt gatgcagttt ctgcaaagct cagggaaatg
    10741 ggtgtggaaa ttacggaata cgaagattcc gtaagagtta aagccccctc aagaacaaaa
    10801 gcggttaatg ttaaaacaat gccctatccg ggtttcccta ccgacatgca gcagcctatg
    10861 agcgtactgc tttctgtttc cgagggcacc ggaatcgtaa cggaaagcat atttgagtcc
    10921 cggttcaagt attttgatga gctgaaaaga atgggggcaa aaataaaaat cgaaggcagg
    10981 gtagccgtaa tagaaggagt ttcaaagctg actggtgctc ccgtttatgc caccgatctg
    11041 agagcagggg ctgcaatggt tgttgcaggc cttactgcgg aaggaaagac taccataagc
    11101 aacatcagct atattgacag aggctatgaa agattggagg aaaagcttat aaaccttggt
    11161 gcaaaaataa ggcgcatcga ggaaaagtag gaggtatata tttgaagctg aaacaaagtt
    11221 tatccatact gcttgttttc tgttttttca ttgttattct aatgcccggt accgcagcgg
    11281 cggcagcatc gggcaccaaa atacccggcg ctttctggag cctggttacg ccttatcaaa
    11341 atgctgtgga tgccaacgac ctgaataata ttattgttta tggcaataaa ataatagagc
    11401 tgttcaatgg aatggaagaa acccagcaag tactggaaat cataactccg cgcctggatc
    11461 aagttgcaaa agcttacgaa gcccttggaa gattcgactt ggccgttgaa tatttcagca
    11521 agtacatccc cagagccgaa aagctgggct ggaaggatgg agtaacttat gcaaggtcaa
    11581 aaatcaatac actgggattt gatattgagc tttatgcaaa aaccacaaac ttggaaaaga
    11641 gtgtttactt cggttcaaaa tatgaaccca ggtcaggggt atattttggc agcaattatg
    11701 ataccgaccc gcggatagga acttatagct ggaatgatat caaaaattat tttcccagga
    11761 agaataccgc ttaccttaca tacctgcatt ggaatgagga catcaagtcc tttgaccgtt
    11821 attttaagga tgcaaaagca aacaacatag ccgttcaact ggcctggaac attgaagatt
    11881 cagaaattga caatgtactt aaaagcataa aaagttatga ttcatatata agagccacgg
    11941 cagattacct gggaaagctt aatatacccg tattcctcag atttgccggt gagatgaata
    12001 taaaagacaa ctgcaaagat tccaattcat atatagcggc cttcaggcat gtttccggaa
    12061 ttatgaaggc caaggctccc aacgtggcta tggtatggtc tcccaatgat atttccgcca
    12121 agggccgcac ctatgaccaa tactatcccg gcgatgagta cgtggactgg atagggataa
    12181 gcacatatac acagcgttat ttccagggca aaaaggattg gggaaacctt caggacacta
    12241 atgacagtgt atatatgacc ggaaattatg caaatcccct ggccaagata aagccgatta
    12301 tggatgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg cgccttgaca
    12421 taggcggtgc ggaaactcat atagtagagc ttgccaaagg gctttcccgt gagggcttta
    12481 atgtaattgc ggcatccgcc gggggcgtgt acgaaaagga gctggccgaa tccggcatca
    12541 aggtatatca cgtgcccctg gacaacaaga agcctcacaa tgtgataaag gcttacttca
    12601 agctgaagaa tataataaaa gaaaatagaa tagacctggt acatgcccat gcccgtattc
    12661 ccggatttat atgcggcctg ctgcataaaa gaatgggatt tccttttgtg actactgccc
    12721 acggaacctg gaaaaccggt tacggattgc gctacataac caattgggga cagaaaaccg
    12781 tggcggtaag cgaggatata aggcaatacc ttcttgataa ctataagatt gaggataagg
    12841 atattaaggt cacaataaac ggaattgata ccgataaatt ctcctccgga atcgattgct
    12901 ccgatataat ggatgagctg aatctcaaac cggaagacat gaaaacagta tatatcagca
    12961 gatttgacag tgacagaagc tatataataa acaagcttct ggaagtaatt cccgagcttt
    13021 cggaaagcat cccaagcctt aaggttatta tggtaggcag cggaaatatt ctggactcga
    13081 taaaggagaa agccagggag ataaataaca taacagggca gaatacgatt attgttacag
    13141 gctcccgtac cgatataaac aaattcaccg ccctggcgga tttattcatc ggctttggcc
    13201 gctcctcact ggaagcactt tctgccggga agccccttat tcttgccggc aatgagggct
    13261 atataggaat attgaatgaa aacagcctgg atgatgcaat caaatccaat ttttccggcc
    13321 ggggaaagag caaagcagac agtaaatcct tcaaggatga tattttaaag gtgctgagtc
    13381 taagtggcga agagagaaaa gccctggggg actttggcag aagtgtgatt gaaaacagtt
    13441 attccgtaag aaaaatggtg caggacaata tggaagtata tgctcagctg cttgaacata
    13501 aataaagggg acaaacctat gtatgacgtt atgatatcag gttattacgg ttttggaaac
    13561 agcggcgacg atgccatact gctggctatt attgaaaacc tgagaaaaat aaaaaaagat
    13621 atacgaattg tagtgctttc aaaaaatccg aaggatactg catcaatcta cggtgtggat
    13681 tccatagatc gttttaacct ttttaaagtc atcggtacaa tgaaaaaaac caggcttttt
    13741 ctgaacggcg gcggaaacct tatacaggat gttacaagca cacgttcatt ggtgtattac
    13801 ctgactacca tttatcttgc caagcttatg gggcttaagg tcatgctgta tgcaaacgga
    13861 atcggcccgg ttggcaggcg caccaaccgt tattttactt caaaaataat agacagagtg
    13921 gatatcataa ctctgaggga ggaagcctca ggcctggaat tgaagtcatt gggcgtcaaa
    13981 aaacccgaaa tccgtgtcac tgccgaccct gccttgggcc ttgaaccggc aggctctgaa
    14041 gaaacagaaa agctgctgca aaaagaagga atacccgcag ataccccttt gattggtttt
    14101 tccgtcagaa agtggagcgg ctacgaggca tacagccgca ttcttgccca agctgccgat
    14161 tacatagagg aaacctacaa tgcgaaatca gttttcatac ccttgcattt tccttcagac
    14221 ctggcagcag cggaggatat tgcatcaaaa atgaagcatg ctccctttat actgaggaat
    14281 atatatgaaa cggataaaat tctcggaata atgaaaaaga tggatttagt gctgggaatg
    14341 aggcttcatg cccttatata ttcggtcagc ctttcagttc ctgttatcgg gcttatatat
    14401 gaccccaaag tacagggctt ccttgaatat gtgaatcagc cctgggcagg caatgtggcg
    14461 gacttgaatt tggatagcct taaaaagctt attgacgaag tatggagcag cagaaaagaa
    14521 atatcacagc agctggaaaa gggcaatagg ctgctaaagc aaaaagccta tgaaaatgcg
    14581 gagatagcaa taaagctcct tgaacaatag caaaaaaact gcgcagcaat tttctactgc
    14641 tgcgctttgc tcaaattctt cacgtctact atttttgcca ccatttctat catcctggta
    14701 tccatcacct gctgggattc cctgttgata tccaacctgc cgtttgccct gaagccgtat
    14761 tttgtctcta ttcccggtac cctgattgtt atcagtacat taaacctttc aggtttttcc
    14821 gctattacca tggtgccgtc ggcctttgta agctggtctg tcgcaggcaa tacttcaatg
    14881 tcggtaatac tccccatagg gctgttggtt ttgacgtcat aaatatccgc accgatttct
    14941 atagcatcca cagactgctg cctgacttcc tccaccagta ccttatactc aatggtatca
    15001 aactttgcag cattctgatc cttgtcaacc ataaaaaatt tataatacac ccccccgata
    15061 agtacagcaa taagcagcaa tacacctata tcaacaatac tgattttgcc gaacaatctt
    15121 cctttttcat ccaaaagcat atcaatccct ccttactgcc caaccaaggt cattttggtt
    15181 acgaagccct gatttgcata tcccttgcct ttaatgaaaa acttcttgcc caccttcact
    15241 tcggcacctc cgattattat actcgccgga gttatattgg catcagcctc tatcgttatg
    15301 acaacgtcat aggcatctgg cacttcagcg atgatatatc tgccgttaat caggtcttca
    15361 ttgagcattg cggcatccct tgaggacttg ctaacaaccg tccccagttc attccccctg
    15421 gtgctgtcac gtatcgggtc tcccggagtt atcgcatcca caaagtcctt gttgacattg
    15481 gttatttcaa agtcatacac tacctttccg gtatttaccg cctgcctgct gttgcctccg
    15541 gaaaaaaaca tatagccgcc ggccgcagtc aatattatca gcagaattat aatgagatcc
    15601 ataatattta ttttcccaaa cagcctgccc ctgtcatccc gtaaagccat atccttcatc
    15661 ttactccctt ctgtttttcc ttgccacaga ttcatacaat tcttctattt tttcagccat
    15721 tatttcggca gtaaagcttt tctccatgat ttcccttccc ctgtcgccca ttgtctttcg
    15781 cagttcagga tcatgaatca gcttaagtat cgcatcagcc agcctttcgg catcccccgg
    15841 aggaacaaga aatccgttat atccgtcctt aaccacctcg ggtattcccc ccgtatctgc
    15901 agctacagac ggcttgccga cactcatccc ttcgattagt gacaggcata gggcttcaga
    15961 taaggaagaa atcacattaa tgtctataat attaaatatt tcatatatct catccacgaa
    16021 gcccgtaaaa actactttat cggtaagccc cttctgctta ataagctcct tcagttcttc
    16081 ttccttcggc ccgataccgg caataaaaaa cctgacattg tcattgtccc gggaaataat
    16141 ttcagctgca tccataaaat atttgtggcc tttgacctcg gcaaggcggg ctacaattcc
    16201 gacaactact tcttcttcac caattcccca ttttcccctg gcaaggcttt tttctttatc
    16261 atcaagtact ttaaccgggt ttatgccccc atatatcacc gatatcttat catcgggcac
    16321 cccgttttcg gcgatattcc gttttacagc ttccgatacg gcaattatgc cgtcactata
    16381 cctgtcgttt atcaaagctc ccgcaaacct cctgataccg gttatagggc tgtcaataca
    16441 atgcttggta ttaattattc ttgctcctgc cctctttccg gcaagccttg cggacaacga
    16501 tgcatgggta tgtacaatat ccggcttttc tgccctgaat atataataca aaagatttac
    16561 cgccctggca tcgtaggatt tgtctttaat gccgtcaata gctattactt caaagcctaa
    16621 gttttttatc ttatctgtaa gcatgcttcc ccgcggaagc acaaccttta tttcaaactt
    16681 ctccttatct attgccccaa gcagattaag cagccagctg cctgccccgc ctattttcat
    16741 atcacttaat acatgcatta ccttaatcat atgcccgaca cctcattatc ctccgtgata
    16801 agagccttgg ttatcacacc gaaggccagc atgacccaaa aagtcagaag caccctgttg
    16861 ttgtaccata tattttcaac cattccctgg gcaagataac ctgccatgcc ggatgcaacg
    16921 gctatcatga aagtggaaat gaacttgtct ttggttttat agaagcctgc cagtaaagtc
    16981 ttgtaataaa ccgctattat tgcaaaaaac atcaataggc ctgctatgcc ggtctctatc
    17041 ataagctgta tgtatatatt atgggaatgg tgggcatatg cggcgttaag tgaatacttg
    17101 ggataaacca gcttaaaggg ctccaggccc aatcctatgc ccgaaagcca ataatcctgc
    17161 gccagcttca aagaccccag ccatacggaa atcctgtaga aggaagatgt atcgttcaga
    17221 tttccgatac tcgcaaacct gttgattaca gaagccggca atacaaaggg aagcatcagc
    17281 agtccgacta ctccaagaat tacgaacctc ctgtccctga gcactgcaaa caaagcaaaa
    17341 gccagcatca atccgacata tgcacctctt gagtaagtat agacgaggca tccgagcata
    17401 atagccgtca accccaggat tgcaagcttt gataaccaat tcttctgtcc ccacagcatg
    17461 gcaattgcaa taggtatgat gagtaccagg tattctccca gcacgttggg gttttcaaag
    17521 gtggagccta cccgggcctt tatgtcctcg aacagcgttg tatccaccca cgcttcggag
    17581 gtagctccca cagtcttcag ctgatatagt ccatacagag atgcaatggt tgtagaaagc
    17641 accagcagtg ctgccaaaaa ataaagctgc cgcctggtct taattgtact taccaatatg
    17701 aaataaaaca caataaatgc catatgtatc aataatgcaa gcatgctgct gccgggagta
    17761 tatgatgtaa tgaagctgta gagaagcacc ataccgaaca atattacgaa ataatcaaga
    17821 attgtgctcc tgagcttcat gctcttatcc ctgaaaagcc tgataaaaaa cgataccgcg
    17881 gtgagcagga tcagccccag cagagccatg gtcggagcca aaggaatgaa ccccgcaacg
    17941 gcaaaaactc ctatctcata cctccagagc accaatacaa gagcggcaat tcctccgact
    18001 gcgagagcaa aggccatcgg ctgcagaaaa tgccacatcg ctcccaatgc tgttcctgta
    18061 acaatcgcag taatttcaaa tcttttgttt aaaactgcat cttccgttct tttatcataa
    18121 aatgcatcgt gagatacgtt aaaaaaacca aataccgcct tgctgactat actgccctcc
    18181 gccagggcct ttaccggcct gtttatcaat atcagcaccg caccgcccag gaataatgcc
    18241 aatttcatta tttcactcat accaaagagc attcctgcat ttctagaagt aaaatccaaa
    18301 agcccaaaaa ctgcggagaa ggtaatgaaa agcagcccgt aaaccctcgt gctcatatca
    18361 aacaggttct ccggaaaaac ccttatggcc ttcagtaccc tgctctcttc tatagcaacg
    18421 tttacgctct ctgagaattt ttgtgaagca aatctgaacc accttaaggg agatacaagg
    18481 attctgaata caaggctttg cctccatgta tccgcgatgt tccattccct gtcaaaaaaa
    18541 ttcaatatgc tgcttgaggc tgcaagcttc ccgaagccat gccccaaacg ctgcataaca
    18601 atgaaaaaca tgctgttttc atagtaaaat acaatcctgt catatacttt caatatcatt
    18661 ccgaatatca agctttcttt cattttttca cttcctgcat gttcagcact aaacgccttc
    18721 tctgattcat atttcacctt attatattat tattgccgcc taaatgtaaa gtaaatacaa
    18781 aaacggacaa cactaaagcc accaaaaccc aacctgcttt ccgtggttta tccgtgcatc
    18841 cggtgttatc cgcgtatttg cctgcaagga ataccccagt tccctccaaa accccgtaac
    18901 ctcgcaacct cgaaacctac tgtaaagaat aagtaccttt gattataaga aatgatttgt
    18961 ccgcaagtgc ttttaaccct ctgccttctc ccctggccgc tatcagcata tggttattga
    19021 caacagcctc gccgttctta agatcctttg ccgcagtaat atccgaaagg cctccgaaag
    19081 catcttctat agcggaaaac ttcccggatc tgggtattat ttccgcacct tcccctgcaa
    19141 taagcatctg gcctttttgc atctccacta cttgaaactt ccccgccgcc tttgcggcat
    19201 cttttatttc ttctctcagc ttggctatct cctgctcctt cagctccaat tcagtcttaa
    19261 gcttggctgt ttcctgtgca atcaggctgt caatataata cttgatctgc tctgatttct
    19321 gttctacata actctgtgta acgatgggat cctgttccga acctggggtt accccgtctg
    19381 cagcgcttat aacaacggca gagagcacca ggataattgc agtcaatact ataatactcg
    19441 ttttgcgact cttactgttt accatcccaa ttctcctttt ctgaggcaat tgcgaaaaaa
    19501 caggtgcttt ttttcacatc tgcccataaa atccaataaa atataatatc ttcaaactta
    19561 gtatatattt cgtcaaatat cgcaaaactc ctgtatatgt tacaaatttg taatttttat
    19621 cttacctgcc cgttaccata tatgatgtac tttgtggata cgttaatata tacggcggca
    19681 tgcgtcaggc ttttcacctg cagctcagag gccgggttaa tatgtcaata attcttgaag
    19741 gcacctttat ttgctcaata ccgaatcaat tatatcaatt gccctgtcaa tatattcctc
    19801 ttgaatgttc aatggcggaa gaagcctaac tacatccgcg ccggctccta caagaagcag
    19861 tcccgaatca atgcattcat cgataaattt tttggtatcg attgaaagct ggagccccaa
    19921 aagcagtcca atgccgcgaa tgctctttat cgagccgtgt ttttccttca aagcctgcag
    19981 cttgccgaca aaatacttgc tcatatgctc aacattttcc agcactccgt tttgcagaag
    20041 ctcctcaagt attgtcaacg ctactttgca ggcaagagga ttgcctccga aggttgaacc
    20101 gtgatctcca taagctatgc attccgctgc cttccctgat gcaacaaccg cacctatcgg
    20161 gaagcctccc cccagccctt ttgcaataca taccaaatcg ggtttaacac cgaacttttc
    20221 aaaggcaaat aaactcccca gccttcctat cccgcactgc acctcgtcga atataagcag
    20281 ggaatcgtat ttttcacaga gtttttttgc ttcctccaaa aatgatttgt cagctgccaa
    20341 tattccccct tctccctgga ccggctctgc aataattgcg catacctttg gattcatttc
    20401 tgctctcagc tggccggcag cattgaggtc aacgctcttt actcccggta tcagcggcat
    20461 gtaatccttc tgatatttct cctgtccggt cacggaaagt gcccccaggg tcctgccgtg
    20521 gaaagagtta ttcagataaa ttatttcatg ctttaaggca ccccctttaa gcttgccgta
    20581 cttcctcgca aactttatgg cagcctctat tgcttccgtt ccgctgttgc agaaaaatgc
    20641 cccttcaagc ccgcttaatt cacacagcct gtcagccagc tccatctgaa gctcattcca
    20701 atacaggtta gacacgtgca tcagcttgca gctttgttcc cggatggcgt ttataatggc
    20761 agggtggcaa tggcccaagc agtttaccgc tactcccgac acaaaatcca agtattcttt
    20821 cccattaata tcataaatca ggcatccttt accgctgact gcggtaacat caaaccgtgc
    20881 ataagttttc agtatctttt ccttagacat tttcctctcc tcctataata attgtccctg
    20941 cctctttgcc atggaaaagc tccagcagaa tatggggctt ctttccgcct ataaggcata
    21001 ccgactttac tcccttttca atagcttcaa tacagcattt catcttgggt atcattcctc
    21061 ctgatatggc accggcctgt acaagctttt caatttccgc agttgtaatg ctgctaatca
    21121 agctgtcggg attgccaata tccccgaaca gcccgtcaac atcggacaaa tatacaaggc
    21181 tgtccgcacc gatagaagcg ccgatagcag ctgccgcata atccgcattg atattataag
    21241 ctttgccttc tttgccttcc gccaccggtg atataaccgg aacatacccc aggctcaaaa
    21301 gatccgtcaa tatttttccg ttaaccttgg ttacttcgcc tacatagcct atatcaatat
    21361 tactgccgtt gtcattcagg tacatccttt ccgcctcaag gagctttccg tccctgccgc
    21421 ttatacctac ggcatctgct ccctttccaa tcagttttga ggtcagctct ttattgaccc
    21481 tgccggacaa caccatttca accacttcca tggcagcctc atttgttttc ctcagccctt
    21541 ttacaaattc ccccgcaata ttcagcctgc caagcatcct gtttatatca ggcccgccgc
    21601 cgtgaacaat cacaactttt acaccggcat ttctcaagat tgccgcatct tccagaaatg
    21661 cgtccgttgc ttccttgtcc ttggtaatgc ttcccccata tttaataaca actgtcccgc
    21721 ctaaatccat tcggccttca ctcctaattc ttaatggtac ttattataca tcgtattgga
    21781 tttttatgca atattattta tgtattttta ttatttctat tgcataaata accgtcccca
    21841 tgtataatat ttaaaaagcg caatattgag gtgattaata tgataaacgc aggtgtcgtt
    21901 ggttcaacag gttatgcggg ctatcagctt tcgatgatat tgtcccagca taaaaatgta
    21961 tctatagttt ttttatcttc tcataattat tctaatttga gattcagtga tgtctatgga
    22021 aatctgaagt atttattgga caatatgtgc atagatatgc aagatataga aagcaaactt
    22081 tcaaaaatcg atgtattatt tcttgcactc ccccacggcc aatcctttga cctggtggaa
    22141 aaagcccttg gcctcggagt gaaggtcatt gatattggag ccgattatag gcttcgggat
    22201 caaaaggtct atgagacgtg gtacggtgta acccataagc tccccggccg cttgaaagat
    22261 gccgtatatg gccttcccga gctttaccgt gacagtattt caaactcaag ccttgtggca
    22321 aatcccggct gctatccgac cgcaagtatt ctggctcttg cgcctttagt acacgccggt
    22381 attattgata acacctcaat cattatcgat gcaaagtccg gagtctcagg ggcgggcaga
    22441 agtgcaaacg tctcatcact tttctgcgaa tgcggtgaat ctgtcaaggc atataatatc
    22501 gcaacccaca ggcatactcc ggaaattgaa cagcagcttt ccgatgctgc aggcgcaaaa
    22561 attaccctca gcttcacacc acatcttgtg ccgatgagca ggggaatact cgcaacttgt
    22621 tacggcagcc tcaagaagga tatttcgcag agcgaactct acgacttata cagaagctat
    22681 tataaaaatg agaatttcat cagaattacc gactccctgc cggaaacaag gtttgtaaaa
    22741 ggatcaaata tctgtgacat tgccgtaagg gtggatgaga ggacaaacag ggttatagca
    22801 atcagcgcaa tagacaatct tataaaaggt gccgcagggc aggcggtgca gaacatgaat
    22861 atccttttcg ggctatatga gcatacaggc ttggatatgc tgcccaatat gctgtaaagg
    22921 agaatgtata aatgaagatt attaaagata tgaccataac agatgtaccg tatttcaagg
    22981 caagcggtgt aagctgcgga ataaagaaaa acggcaataa ggatttatgc gtaatataca
    23041 gtgagaagga ttcggttgct gctgctgttt ttacaacaaa caaggtaaag gcggcgccgg
    23101 taattcttga tatgaagcat attcagtcaa aaagcattcg ggcaattgtt gcaaacagcg
    23161 gcaatgcaaa tgcctgcaca ggaaaacagg gtcaaaagga tgcaaataca atggcagagc
    23221 tgactgcctc cctcctgaat attccctctg actcagttct ggtagcgtca accggagtta
    23281 tcggcgttcc tctcccgatg aaaaatattg aggcaggaat aaaaaatgca tgtgataatc
    23341 tgtcctatga cggcggtaag gatgccctcc gggcaattat gaccaccgac acatgtgaaa
    23401 agtcagtaac cgtcgaagtt gagcttgaag gtaaaaaggt gctgctcagc ggtattgcga
    23461 aggggtcagg catgatacat cccaacatgg ctacaatgct tgggtttatc accactgatg
    23521 caaatatatc aaaggaaatg ctccaggagg ccttgagcga aagtgtaaag aaaacttata
    23581 atatgatatc ggtggacggg gacacaagta ccaatgacat ggtaataatg cttgccaatg
    23641 gagccgcgga taacaaactg attggggcca gggataaaaa ctttgatgct ttcatgaagg
    23701 cccttgacct cctgaatacc gagcttgcaa aaaagattgc ccgtgacggc gaaggcgcca
    23761 caaagctgat tgagtgcact gtctcaaacg cagtttccta tgccgatgca tgcatacttt
    23821 caaaatcagt tatcggctca aatttgacca aagccgcatt tttcggtaat gatgccaact
    23881 ggggaaggat cctgtgtgct cttggctact ccggcgggaa tttttccccg gataaggcgg
    23941 atatttattt tgaaagcgag gcaggctgca tccagcttgt ggccgatggg gagcctcttc
    24001 agttttgtga agaaaccgct tccgcaataa tggacaaaga atatgtaata ataaaagtgg
    24061 atttaaagga agggggatat tcggctactg cctggggatg cgaccttacc tatgattatg
    24121 taaagataaa cggaagctac agatcataag agacagaggg acgtttcgag acacggggac
    24181 gtttctcctg gggacatagg gatgtttccc ctgtctcaca atagaagaat aatgcgtcgc
    24241 attcttcggg tacgaggtac gaggcgcgag gttcgaggtt taggg
//
LOCUS       DAOQUR010000034        22592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2683_length_22592_cov_10.596885, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000034 DAOQUR010000000
VERSION     DAOQUR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_22592_cov_10.596885"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..902)
                     /locus_tag="PKI50_08170"
     CDS             complement(69..902)
                     /locus_tag="PKI50_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNT03712.1"
                     /translation="MNKPKVALIQMHVEEDKLRNMVKASDFIRKAAEQNIDIAVLPEM
                     FNCPYKTSNFPVYAEEEGGECYGMLSELARKYQIYLIAGTMPEKDDKGRVFNTCYVFD
                     RKGNKIGKHRKMHLFDIAVEGGQHFKESETLTAGNTVTVFETEFGKMGVAVCYDFRFP
                     ELSRLMVEEGAKVLIAPAAFNMTTGPAHWEVMFRSRAIDNQVYTIGTAPARDVDSCYT
                     SYGNSIIVSPWGEVVARLDEKEGLLIETLNLDYVEKVRRELPLLAHRRKDIYKCGKKE
                     E"
     gene            complement(971..2965)
                     /locus_tag="PKI50_08175"
     CDS             complement(971..2965)
                     /locus_tag="PKI50_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNT03713.1"
                     /translation="MIKNFKLSLQIGAVFIGTVVGAGFATGQEIMQFFTCFGQIGAVT
                     LLLSGILFYLMAAAVIRAAAYYNAYNYKELIYQVAGNRIGFVYDILVTAFLFIGTSIM
                     FAGSGALFHESLGLPHTLGVVLMAVLTFIIILHSLTGILNINSVIVPILFAVITAVLA
                     ATIMNGDIGGFGLRLSENYDGNFFKPVFYFLFFCSYNTFLSIGVLSAIPEKVKNLPVL
                     KTGVFLGAMGLMLLSLMLNISLTLKSPQVFNYSIPMDCITSGLGRIIKSVVALCIWCE
                     IFSTAVSNAFSIAKRLSKNRHITYKQTCFFTVLCCLPLAFLEFKGLISFFYPLFGALS
                     MFIIFRLLFASYGFGGKTKKVISLVSILCMITVFAYIANNPQPCFTYIEPVESSYDIT
                     MKQDLLCLMMAYPEHIASIEKLEDRVYVTMKSGRKILYDDKKIKDMEAKIAYPDLQDM
                     MEPVYPIGLVKKLMDINYDPGRARVYSLLNEVYGGSRQQIEGNLVNVNTGCGAFQFNR
                     NNKAGEAFKNVMDELASAAKNRKEIRACVYPCSGTYNYRVISGTNRLSPHSYGIAIDL
                     ARDKRDYWQWASREEGQKRLSSYPNEIVELFERNNFIWGGKWGHFDILHFEYRPEILI
                     KARYFGNSRDIHKPWYNEVLLEDAYAAEYIRKIDKALDGN"
     gene            complement(3034..3951)
                     /locus_tag="PKI50_08180"
     CDS             complement(3034..3951)
                     /locus_tag="PKI50_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HNT03714.1"
                     /translation="MKKITNISIIGLGAIGGSYAERLYQNNNVNLTVVADRSRIEKYN
                     TNKLTVNGKPCDLKFIQPDSDEAPADLVLIAVKYNNLSQAVDAIRKRVGPDTVIMSLM
                     NGIDSEEIIGEAFGMEKLLYSSCVGIDALREGNNIRYTSFGKLHFGERENKLHTERVE
                     RVKVLLDESGIPYHIPENMIKALWWKYMVNVGLNQVSAVLGAPFRVFNTVQEIRELVD
                     AAMKEVIDVSIKAGINLEEESMKEVHKLVDTLSAEGKTSMLQDIEAGRKTEVEMFSGV
                     LRKIGRKYGVPTPVNDTLYRMIRTLEQME"
     gene            complement(4084..4587)
                     /locus_tag="PKI50_08185"
     CDS             complement(4084..4587)
                     /locus_tag="PKI50_08185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNT03715.1"
                     /translation="MKAGSIQLRQEVYQADAWKIADWLDDYEVIKYLNEGRNVVKSIT
                     EAIQRVNMPILTHLFNQKGSFFMIMEKKQPIGFLRLVPKGKTAEMVIVIGEKEKWGRG
                     IGKHAIMEGLHHAFFEWRMDEVIAKINLKNERSIRAFKSVGFKPEKELMKEMQYSISI
                     REFLKIG"
     gene            complement(4872..5765)
                     /locus_tag="PKI50_08190"
     CDS             complement(4872..5765)
                     /locus_tag="PKI50_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT03716.1"
                     /translation="MAVNFKNLPDEELLYGAKTCQGCGVTLAARLALKVLGEKTCLAT
                     PACCFAATTTVYPQSSIFVNNAITAFPALASTLSGMAVAAEALGWDEDITILGIAGDG
                     GTVDIGLQALSGAAERNENIIYICVDNEAYMNTGNQRSGVTSYKAWTTTTPTGSCSKG
                     EKNKFKKSLFEIMAAHRVPYTATASVAYPKDFMEKVEKAKNIKGCKVIHVMTPCPTGW
                     GYDPAITVKTAKLAVECGLWYLAEFENNEFRFNFVPKEFKPVEEYLKLQRRFRHLDES
                     DYREIEANRDIEWDRMRKRFA"
     gene            complement(5765..6937)
                     /locus_tag="PKI50_08195"
     CDS             complement(5765..6937)
                     /locus_tag="PKI50_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNT03717.1"
                     /translation="MSKKAFLSANEAAAYGVKLSRPHVVSAYPITPQTTLVEKIADFL
                     ANGEADFKYMMVESEHSAMASALGASMMGARTFTASSSQGLLYMCEMLSYVSGSRFPI
                     VMVDANRSVATPWNIFGDQRDAMAMRDSGWIMLYVESGQEALDTVIQAYKIAEDPEVM
                     TPVMINIDGFTLTHTYDMVEIPEQEEADSFLPPYSTTNKMDMKDPKTLCMSVGPDFHT
                     EGRFLQQKAFEAAKRKIVSVNDEYAKLTGRSYGGMIEEYRCDDAEAVLVAVGSVAGTV
                     RVAVDEMREAGKKVGLIKLRSFRPFPKEYFESIRDKFKAVGVIDRSISFGFDGTVFSE
                     VKSAMYGSGAKMANYIVGLGGRDIPKHQIETMFSKLLGMASGKNEAEVQFLGMRWE"
     gene            complement(6934..7242)
                     /locus_tag="PKI50_08200"
     CDS             complement(6934..7242)
                     /locus_tag="PKI50_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT03718.1"
                     /translation="MNKPKWNKDYKAPSSWDEMFFGSSNGVGILPTPNSGFRTLHPEI
                     DSKACIKCMMCWVFCPEGCIDKSGDHLEIDMDYCKGCGICAAECPKNCIKMIKDGDTE
                     "
     gene            complement(7242..7808)
                     /locus_tag="PKI50_08205"
     CDS             complement(7242..7808)
                     /locus_tag="PKI50_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT03719.1"
                     /translation="MKQIRWHGRGGNGAFTAARLLGVAASVFEGKSAQAFPSFGPERR
                     GAPVLGFTRIADKTITDHSQVYECDCVIVLDETLCDVTNVAAGLKPDGVLVINTRKTA
                     DEAARHIGFEDPSRIVVLDATDIALELLGNPIVNTIMLGAAVGATEMVSMESVEKAID
                     DVMGRNLREKNKKAARAAYDKVKEAVMQ"
     gene            complement(7805..9259)
                     /locus_tag="PKI50_08210"
     CDS             complement(7805..9259)
                     /locus_tag="PKI50_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HNT03720.1"
                     /translation="MALKTGAQYIEEMKKMRPNVYKWGELIEDVTEHPATASHIRNVA
                     KWYDNSFDPEKEQLYTNTSFLSGNKAHRWNTLITSADDMLGNANMKRDGYHTCGSCMG
                     PVCAGWTVLNALWSVTFDMDQEFGTDYHERLKNFCIFAENNALALSGALTDAKGNRAL
                     KPSQQDNPDMYLHVVERREDGVVIRGFKNQICGTVGSQYIMVMPTTGMGEDEGDYAIA
                     AAVPRDAEGVTIVETRRPSDTRIEEEGWDGIKSGTTQAYIIFDNVFVPSKHVFMNGET
                     KYTGKLIGNFTAIYRAAIGACVAGQGDVMIGAALGMARANGLAQKAFQDKLTKMAINN
                     ETTYGLGIGAMYTGKKHKSGAFYPNPLLAHVNKVHVATLPYDTKVLAQEISGGITETG
                     CMPSYKDMMSPLYGEKLIESLRSAVPGEDRIKMARLVEWLTIGGGVPGCMHGGGSPDT
                     AKMVVKAATKWDNYVDYARALAQVESPLKEEKKK"
     gene            complement(9273..10259)
                     /locus_tag="PKI50_08215"
     CDS             complement(9273..10259)
                     /locus_tag="PKI50_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNT03721.1"
                     /translation="MKKIWVFAEYVNCTLDRVTLELLTKAKELASGLKGETEVAAVLI
                     GSRIGSTCKTLAEYGAEKIYLVESDELSLYNPMLYAPIITELVQKEDPDIFLFGATAI
                     GSELGPTAAAQLKTGVAAHCVDMHIDENEHLVAAVPSFGGKILGEILCPDTRPQMASV
                     KPGIFVQGEPEEREAQIIKVDVSAITADNLPLKAISLEKKETEGVPLDKAEVVFCGGY
                     GIGCKENWGKLEKLAAIFGGAAACTRPVVDEGWAPGEHVMVGTSGKSIRPKAYVGFGI
                     SGATHHICGMKDSGLIISINKDEKAAVFEVSDAGAAADLNRILDQLMIKLDL"
     gene            complement(10256..11104)
                     /locus_tag="PKI50_08220"
     CDS             complement(10256..11104)
                     /locus_tag="PKI50_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT03722.1"
                     /translation="MALKIVVCIKPVPDPDYYDKVSIDPVTKRITREGIPTIINPVDK
                     NGIEAALKLKAQYGGKVTVLTMAPPNATENLKEALAMGADEAVLLSDRAFGGADTLAT
                     SYTLAKGIEKLEGFDIVFCGTESADGATAQVSSQLGEWLGVPHLWNVFSLENLEERTL
                     RVRTKAENGFMEWEVKLPCLLGVSRELNKPRFISAMGIIKAKNKPITTWGKADLDAEE
                     ERLGLAGSPTKAGAIFTPDLKRKGEMLTGSAEEIADAIIEKLRTAGVNIPTKGDACII
                     EGGARQ"
     gene            complement(11145..12299)
                     /locus_tag="PKI50_08225"
     CDS             complement(11145..12299)
                     /locus_tag="PKI50_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT03723.1"
                     /translation="MQFFEFTEDQELLRKAVREFAEAEVAPKAAEWDENDYCPMELFP
                     IMGELGINGIFAPEEYGGAGMGHVERAICIEEISRYSAGLGIALMTHHLGMSPILDFG
                     TEEQKKKYLPELCAGTKLCGLAVTEPGGGSDFAGQKTTAELVDGQWVLNGRKCFITNH
                     HADVNVITAVTGQDEKGRNQLSAFIIEKGTPGFGAGRKEHKMGLRGSVTGDVILNEVK
                     IPQENIIGNVGAGAKIGMAAIGEVGRAGMSAICVGILRGCVEEAVKFANERVVYGKPI
                     AKLQAIQFEIANIRTEYEAAKMLLYYAAGKKDAKLPATPDFAIAKYYSTEAAVRASKR
                     TMDLMGGYGVINEYPVGRFLRDALASIPSGGTSHIQQLIIAGNTLSAFKA"
     gene            12572..12985
                     /locus_tag="PKI50_08230"
     CDS             12572..12985
                     /locus_tag="PKI50_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT03724.1"
                     /translation="MMLDECINFLLTKTQQVVFQQFKAGLAKYDVTPAQYGVLKCLWS
                     QDCQNPSQIACSLFLDASTITGILDRMENKDMIKRIADPKDKRSLKVVLTERGRLLEK
                     PVTEIIDEENKKVLHALNEGERKKLAEYLNRISSI"
     gene            13009..13878
                     /locus_tag="PKI50_08235"
     CDS             13009..13878
                     /locus_tag="PKI50_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT03725.1"
                     /translation="MIYDEPLFRPPSEAYSLIFQATIGCSHNKCSFCGMYKSKKFRIR
                     SKSDVFRDLEEMAARYNKVRRIFLADGDALVLKTRDLKDILQKIRELFPCCERVGIYA
                     TPGDILRKSPEDLTSLKEHGLGIVYLGVESGSDKILKAVDKGADSKEIIEAGKRIVSS
                     GIKLSVTLISGLGGKDGWEEHARESARVINEINPDYLGLLTLLIEPGTRMHDLVQQGT
                     FKLLSAEEVALETQLLIRNLELRNCIFRSNHASNYFSLEGTLPHDKSKLLKQLDNILN
                     NIEDYSGEYFRRL"
     gene            complement(13898..14572)
                     /gene="plsY"
                     /locus_tag="PKI50_08240"
     CDS             complement(13898..14572)
                     /gene="plsY"
                     /locus_tag="PKI50_08240"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNT03726.1"
                     /translation="MSTLYNSMINIAVIIILSYLAGSIPTSIIVCKAVKGIDIRKHGS
                     GNAGATNVFRVMGWKTGIFVVFIDIFKGFAATEWISRLNLSGGAPQSSMLVPILAGAA
                     AVIGHCFTIFAGFKGGKGIAASTGMLIALFPVPLLVSAASFAVVLLTSGYVSLSSLTA
                     AIVFPVTMTALYINNTDNIDQTALIFSIIIAVFIFYTHRSNIKRLIAGNENRFEKLRI
                     LAKKNR"
     gene            complement(14597..16018)
                     /locus_tag="PKI50_08245"
     CDS             complement(14597..16018)
                     /locus_tag="PKI50_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT03727.1"
                     /translation="MKNNRVASLSLKAGKAAKDGFLGAESFYIFDDHTDGVCIINERG
                     YIEYFNGAYERILKISDRIETGMNIFLTKNDDVLLTAFREKRCICGHLVLYKGAAHVS
                     ASASPIYSNGEFKGVVAIYREEAAQDICRKKDTVIEFNSPPVNYMNNIRTMFKDIVGE
                     SSSIMKALKIAYKAAQTSSNVLICGESGTGKGLIARAIHNNSSRAKGPYSIVNCGAIP
                     STLLESELFGYERGAFTGAVRRKTGKFEQADGGTLFLDEIGDMPPDMQVKILRVIQDK
                     EFERIGGCDTIRCDVRIIAATHCNLEALVEKGAFREDLYYRLNVLPIYVPSLKERRED
                     IPMLLEYFIKRISINMNKEITGVSPEAENCLLNYDWPGNIRELENLVERVIALTDGNE
                     IDINSLPSKISSLYCVNRHTCSAPALINMNNSGNIATLEEYEKEIIKEALARFGSYNA
                     AGKALGITHKTVALKARRYNIVI"
     gene            complement(16049..16411)
                     /locus_tag="PKI50_08250"
     CDS             complement(16049..16411)
                     /locus_tag="PKI50_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HNT03728.1"
                     /translation="MERNIVTCTICPIGCSLEVIGDSSSDSGYLVKGNRCNRGIGYAI
                     AEVTNPARILTTTVKLNNAILRRLPVRTDKPVPKNLLLDCMKVINSVEVDTPIALGAV
                     IIENILSTGANVISSRTV"
     gene            complement(16424..17674)
                     /locus_tag="PKI50_08255"
     CDS             complement(16424..17674)
                     /locus_tag="PKI50_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT03729.1"
                     /translation="MNEYDIAVIGGGPAGLAAAIEAKKHGGTVLLIDREKELGGILQQ
                     CVHNGFGLHIFREELTGTEYAERFIKEFKELGIEYKLDTMALNITEEKVISMVNKTEG
                     FVNIKARAAILAMGCRERTRGAINIPGTRPSGIFTAGTAQRFVNKEGYMVGRNVVILG
                     SGDIGMIMARRLTLEGARIKVVAEALPYPGGLIRNVVQCLEDYGIPLLLNHTIVDIKG
                     KDRIEGVTVAELDENRKPIKGTEQFYECDTLLLSVGLIPENELSKEADVELDPITGGP
                     VVNESMETTVKGVFACGNAVHVHDLVDYVTEESRIAGRNAARFVNGETGGYGPAIRTK
                     PGNGIRYIVPHFIRKDKVEQSVDLFMRVNKIFTNKKLVVKFGDTVIKIIKKSYMRPGE
                     MERICITAESLKTAEDLSLTVDVE"
     gene            complement(17687..19171)
                     /locus_tag="PKI50_08260"
     CDS             complement(17687..19171)
                     /locus_tag="PKI50_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT03730.1"
                     /translation="MYDIAVIGAGITGCSIARELSRYNVRILLIEKNSDVADGTTKAN
                     SAIVHAGYDAKPGTLKARFNVPGALMYEKLCSELDVPYKKTGSLVLAFSPEEMKTIQK
                     LYDQGIANGVPDMEIIGKDKVQEIDGNLSDEVVGALYARNAGIVSPWELAIALAENAV
                     ENGVELFLDSEVTKIEKMNGLYRLHMGDRLAEARFVINCAGLYSDKVNEMVAAPSFKI
                     LPRRGQYNLFDKSLGNFVNTVIFQCPSDFGKGVLIAPTVHGNILIGPDAEDIEDKEAI
                     QTTPEGISYIWEASARSAKGLKPNAVITAFAGIRARSSTDDFVIGESKEAKGFINVAG
                     IESPGLTAAPAIAVHAADIVKGSMGGLEENLGFNPVRRKVVRFMELSQEDKNLLIKQD
                     PRYGRIICRCESITEGEIVDAIHRKAGARSLDGVKKRVRPGSGRCQGSFCGPRVMEIL
                     ARELDIDIKDVVKNSPKSYILTGETKDNSLYEDVHDLAAASGKV"
     gene            complement(19159..19332)
                     /locus_tag="PKI50_08265"
     CDS             complement(19159..19332)
                     /locus_tag="PKI50_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03731.1"
                     /translation="MLHYYTQDICFQCKALERGVAVTTAIDSNAVVEQTDKYHAAIRC
                     CKVLVLEELKCMI"
     gene            complement(19500..20990)
                     /gene="glpK"
                     /locus_tag="PKI50_08270"
     CDS             complement(19500..20990)
                     /gene="glpK"
                     /locus_tag="PKI50_08270"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNT03732.1"
                     /translation="MSKYILALDQGTTSSRAIIFGNDGKIVSVAQKEFTQIYPKAGWV
                     EHDPMEIWGTQSGVAREAMDKIGIGADDIAAIGITNQRETTVVWERSTGKPIYNAIVW
                     QCRRTAPICDELKAKGLAETIRNKTGLVVDAYFSGTKVKWILDNVEGARERAKNGELC
                     FGNIDTWLIWNLTKGKVHVTDYSNASRTMLFNIHELAWDKEILEELDIPEAMLPEAKS
                     SSFVYGETDKEIFGAEIPIAGAAGDQQAALFGQACYKPGMAKNTYGTGCFMLMNTGEK
                     AVSSSNGLLTTIAWGLNGKVEYALEGSIFIAGASVQWLRDELKIIDNAAASEDMAAAV
                     ADTNGVYVVPAFVGLGAPYWDMYARGSILGLTRGANRNHIVRATLESIAYQTRDVLEA
                     MQEDSKINLQALKVDGGAVANNFLMQFQADILGVPIYRPEVTETTALGAAYLAGLAVG
                     FWDSKDEIAEKWNVDRVFEPVMDSSSRDKKYAGWKKAVSRTLKWEE"
     gene            complement(21048..21746)
                     /locus_tag="PKI50_08275"
     CDS             complement(21048..21746)
                     /locus_tag="PKI50_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HNT03733.1"
                     /translation="MSPFLSELIGAMILIIFGGGVCAAVTLNKSKSQSSGWIVIAVGW
                     GLAVGMAALAAPGTAFNPALTIANAVAGTLDWALVPGIVAGQMLGAIIGAAVVWLIYL
                     PHWAITEDKAAKLGVFCTAPAVRDYGKNFLTEMIVTAVLAFMCLAIGANKLADGLGPM
                     VTAAMITAIGCSLGGPTGYAINPCRDLGPRIAHAILPIAGKGGNDWSYSWVPVFGPIV
                     GAIIGTFLYMGLFR"
     gene            complement(21861..22427)
                     /locus_tag="PKI50_08280"
     CDS             complement(21861..22427)
                     /locus_tag="PKI50_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HNT03734.1"
                     /translation="MSNKFFPLIYKNPIIAAINDLNNIELAAESPCNIVFLLKGDISN
                     IAGIVDRFKSAGKGVFVHMDLIEGFSKDAVSLRYFHDNIKPDGIITTKSNLVRIAKNN
                     GIFIIQRLFALDSLSLASGIESLHTTKPDAIEILPGIMPKVINEVHGQTSIPVIAGGL
                     IRDKEDVIDCLNAGALGVSTSKKELWYM"
BASE COUNT         5486 a         5895 c         4238 g         6973 t
ORIGIN      
        1 ggacgtttct cctgtcccgc tttcagcggg acaggagaaa cgtccccatg tcctcaagat
       61 gtgtcgcatt attcttcttt tttcccgcac ttgtaaatgt cttttcttct atgtgcaaga
      121 aggggaagct cccttctcac cttttccaca taatccagat tcaatgtctc aataagcagc
      181 ccttcctttt catccaaacg tgcaaccacc tctccccagg gagaaactat aatggaattc
      241 ccgtaggaag tataacagga atccacatcc ctggccggtg cagttccgat tgtgtacacc
      301 tgattgtcta tagctctgct cctgaacatt acttcccaat gggccggtcc tgttgtcata
      361 ttaaatgcgg caggtgctat caacactttg gcgccttctt ccaccatcag ccgcgacagc
      421 tccgggaaac ggaaatcata gcagacagcc acgcccattt tcccgaattc cgtttcaaac
      481 acagtgacag tattccctgc cgtcaaggtt tcggattcct taaaatgctg tcccccttca
      541 actgctatat cgaacaggtg cattttcctg tgcttcccga ttttatttcc cttacggtca
      601 aatacataac aggtattgaa tactcttccc ttatcgtcct tttccggcat agtccccgct
      661 ataagataaa tttgatattt tcttgccagc tccgaaagca tgccatagca ttctccgccc
      721 tcttcctctg catatacggg gaagttggag gttttgtaag ggcagttgaa catctcggga
      781 agtactgcaa tatctatatt ctgctctgct gcttttctaa taaagtcaga tgctttgacc
      841 atatttctca gcttgtcttc ttccacatgc atttgaatca gtgcaacctt gggtttgttc
      901 acgcattctc cctccttata atactttgac cattctatga ttcttattat aaggtttaaa
      961 gaatcccagg tcaatttccg tctaacgctt tatctatttt tctaatgtat tccgctgcat
     1021 atgcatcctc aagaagtact tcattatacc atggcttatg aatatcccgg ctgtttccga
     1081 agtaacgggc tttaatcaga atctccggcc ggtattcaaa atgaagtata tcaaaatgcc
     1141 cccattttcc gccccatata aaattgttcc tttcaaaaag ttcaacaatc tcattgggat
     1201 aggaggacag ccttttttgc ccctcctccc tggaagccca ctgccaatag tccctcttat
     1261 ctcttgcaag gtctatggct ataccgtatg aatgagggct caggcggttt gtacctgaaa
     1321 taacgcgata attgtaggtg ccgctgcatg gatatacaca agctctgatt tcttttctgt
     1381 tttttgctgc tgaagcaagt tcatccataa cattcttaaa agcttcccca gccttattgt
     1441 tcctattgaa ctgaaacgct ccgcatccgg tatttacatt tacaaggttt ccttcaatct
     1501 gctgccttga gcctccataa acttcattca gcaggctgta taccctggca cgccccggat
     1561 cataatttat gtccataagc ttttttacaa gcccaatagg ataaacgggc tccatcatat
     1621 cctgcaaatc aggatatgca atttttgcct ccatgtcctt tatttttttg tcgtcatata
     1681 atatcttcct tccggacttc attgttacat atacccggtc ttccagcttc tctatacttg
     1741 caatgtgctc aggatatgcc atcatcaggc aaagaagatc ctgcttcata gtaatgtcgt
     1801 aggatgactc gaccggttca atataagtga agcacggctg cgggttgttt gcgatatatg
     1861 caaataccgt aatcatacaa agtattgaaa ctaaagaaat aacctttttt gttttaccgc
     1921 cgaatccata tgaagcaaac agcagcctga agataataaa catggacagt gccccgaaca
     1981 acggataaaa gaagcttata agccctttga attccagaaa agccaggggc aggcagcaaa
     2041 gaacagtgaa gaaacaggtt tgcttatatg taatatgcct gttcttactc agcctttttg
     2101 ctatgctgaa ggcatttgat actgcagttg aaaatatttc acaccatatg caaagggcaa
     2161 caacactttt tattatcctt ccgaggcctg aggttatgca gtccatgggt atcgaataat
     2221 tgaacacttg ggggctctta agagtaaggc ttatattgag cataagggac aaaagcataa
     2281 gccccattgc gcctaagaaa actccggttt tgagaaccgg caggttttta actttctcag
     2341 gtattgcaga cagcacccca atggaaagaa aagtattgta actgcaaaaa aacagaaaat
     2401 agaataccgg cttaaaaaag ttgccgtcat agttctccga aagccttaaa ccaaaacccc
     2461 cgatatcgcc gttcataata gttgctgcca gtaccgcagt aatgacagcg aaaagtatcg
     2521 gaactatgac ggaattaata ttaagtattc ctgtcagtga atgaagtatt atgataaatg
     2581 taagaactgc catgagtact acccctaagg tatggggcaa tcccaggctt tcatgaaaaa
     2641 gcgcgccgct gcctgcgaac attatggaag tgcctatgaa aagaaaggca gttaccagta
     2701 tatcatatac aaatccgatt ctgtttccgg caacttgata tatgagttcc ttgtaattgt
     2761 aggcattgta ataggctgca gcccttataa cggcagcagc cattaaataa aaaagtatac
     2821 ctgacagtaa tagagttaca gcgcctattt gtccaaagca ggtaaaaaac tgcatgattt
     2881 cctgacctgt ggcaaaaccg gcgccgacca cggtgccgat gaaaactgca cctatctgga
     2941 gcgaaagctt aaaattctta atcatagaaa atctcctttt atggaatagt catatctata
     3001 attatttaag ctttcactgt cctatgaata aaatcactcc atctgttcca aggttcttat
     3061 cattctatac aaagtatcgt ttacaggtgt cggtactcca tacttccttc ctattttccg
     3121 caatacaccc gagaacatct ccacctctgt tttcctcccg gcttcaatat cctgcagcat
     3181 ggaggtcttg ccctccgctg atagggtatc caccagttta tgcacctctt tcatgctttc
     3241 ttcctcaaga ttgatgccgg cttttatcga aacatcgatt acttccttca tagctgcatc
     3301 cacaagctcg cggatttcct gaacggtatt gaacaccctg aatggcgctc cgagaactgc
     3361 agaaacctga ttcagaccca cgttcaccat atatttccac cataaagcct ttatcatatt
     3421 ttctggaata tgataaggaa tacccgattc atccaacaga acctttacac gttccaccct
     3481 ttcggtatgc agcttatttt cccgctcacc aaaatgcagc ttcccaaagc ttgtgtaacg
     3541 aatattattg ccttccctta gcgcatcaat gcctacacag gaagaataca gaagcttttc
     3601 cataccgaag gcctcaccta taatctcttc actgtcaatt ccgttcataa gagacattat
     3661 aacggtatcc ggccctacgc gttttcttat tgcatcaaca gcctggctga gattgttgta
     3721 tttgaccgct ataagcacaa gatccgcagg ggcctcatca ctatccggct gtatgaattt
     3781 taagtcacag ggctttccgt ttactgtcag tttgttggtg ttgtattttt caatcctgga
     3841 tctgtccgcc acaacagtaa gattaacatt attgttttgg taaagccttt cggcatagct
     3901 gcctcctatg gctcccaagc ctatgattga aatattcgtt atttttttca agctatcatc
     3961 ccctttccaa acatcccggt acaatctctt tcccttcaga aaagaaaaac ctgatatgga
     4021 aattatacca catcaagctc tttcattata tatgcataag ccgaaaatgc ccctgtccct
     4081 ttattatcca atcttaagaa actcccttat agagatggag tattgcattt ccttcatcaa
     4141 ttctttttcc ggcttaaatc ctactgactt aaatgccctt attgatcgtt cattctttaa
     4201 gttaattttt gcaattacct catccatcct ccactcaaaa aatgcatggt gcaagccttc
     4261 cataattgca tgttttccta tgcccctgcc ccatttttcc ttctcaccaa ttactatgac
     4321 catctctgct gtcttgccct ttggaacaag tcttaaaaaa cctatgggct gctttttttc
     4381 cataatcata aaaaagctcc ccttttgatt gaataaatga gtaagaatag gcatgtttac
     4441 tctttgaatt gcctctgtaa tgctctttac aacattccgt ccctcgttta aatatttaat
     4501 cacctcataa tcatccagcc aatcagcaat cttccatgcg tcagcttgat agacctcctg
     4561 ccgcagctgg atactgccag ccttcattaa cacaaccctc cataaaaaat tataagccta
     4621 aaatagtaaa tctattatag gctttcccta aggttatggt aatgataaca tcctgttaca
     4681 tttaagtcaa atgctgtaaa cgaagttgcg agagtttaac tgttgatatt gaccaaaaga
     4741 gccatttccc tgactttccc tacccattcc gaaataagcg cctcacgctg tactccatcc
     4801 cttgtatccc tcctatacaa taaaaacaac tgtggcttcc gtcaccacag ttgttttttt
     4861 gcttctcttt tttatgcgaa tctttttctc atcctgtccc attcaatatc cctgttggcc
     4921 tcaatctccc tgtaatcgct ttcatccaga tgcctgaacc tgcgctgcag cttcaggtat
     4981 tcttccaccg gtttgaattc cttgggtaca aaattgaatc tgaactcgtt gttttcaaac
     5041 tctgcaagat accagaggcc gcattcaaca gcaagctttg ctgtttttac cgttatagcg
     5101 gggtcatagc cccacccggt aggacatggg gtcattacgt gaataacctt gcagcccttt
     5161 atattcttgg ccttttcaac cttttccata aaatccttcg gataagcaac agaagctgta
     5221 gccgtgtaag gtacacgatg agccgccatg atttcaaaca gactcttctt gaacttgttc
     5281 ttctcaccct ttgagcatga gccggtcggt gtggttgtag tccatgcttt gtatgaggtt
     5341 acgccgcttc tctggtttcc cgtgttcata tatgcttcat tgtctacaca gatatagata
     5401 atgttctcat ttctttccgc tgccccggac aatgcctgca ggcctatgtc aaccgttcct
     5461 ccgtcccctg caatgcccag aatggtaata tcctcatccc atccgagagc ttctgctgcc
     5521 actgccattc ctgataatgt agaggcaagg gccggaaatg cagttattgc attgtttaca
     5581 aaaattgaag actggggata tactgttgtg gttgctgcaa aacagcaggc gggagtggcc
     5641 agacaggttt tttcgcccaa tactttcaga gccagcctgg ctgcaagcgt tactccgcat
     5701 ccctggcaag tctttgcacc gtacaacaat tcctcatcag gcaagttttt aaaatttact
     5761 gccattattc ccacctcatt cctaagaact gcacttcggc ttcattcttt ccagaagcca
     5821 tacctaaaag cttgctgaac attgtctcta tctgatgttt cggtatatcc cttccgccca
     5881 ggcctacgat gtaattggcc atctttgcgc ctgaaccgta catggcagac ttgacctcag
     5941 aaaatacagt tccgtcaaaa ccgaaggata tgcttctgtc tattactcct acagctttaa
     6001 atttatctct tatagattcg aaatactcct ttgggaaggg cctgaagctt ctgagcttta
     6061 taaggcctac cttctttcct gcttctctca tttcgtccac cgctactctt acagtacctg
     6121 ccacacttcc cactgccacc aggactgcct cagcatcatc gcagcggtat tcttcaatca
     6181 ttccgccgta ggatctgcct gtcaattttg catattcatc attgacagag actatctttc
     6241 tctttgcagc ttcaaaagcc ttttgctgca aaaaccggcc ttcggtatgg aaatcagggc
     6301 ccactgacat acataaggtt ttgggatcct tcatatccat cttgtttgtt gtactgtaag
     6361 gcggcaggaa gctgtccgct tcctcctgct ccggtatctc caccatatca taggtatggg
     6421 taagggtaaa accgtcgata ttgatcatga caggggtcat gacttctgga tcctctgcta
     6481 tcttgtatgc ctgtatgacg gtatccaatg cctcctgtcc gctttccacg tacagcatta
     6541 tccatcccga atccctcatt gccatagcat ccctctggtc tccgaaaata ttccaggggg
     6601 tggcaacgga acggtttgca tcaaccatta ctatcggaaa cctggaaccg ctgacatagc
     6661 tcagcatttc gcacatgtaa aggagcccct gggaagagga tgcagtaaag gttcttgctc
     6721 ccatcatgga cgcacccaaa gctgaagcca tggcgctgtg ttcactttct accatcatat
     6781 atttgaaatc cgcttctccg tttgccagaa aatctgcaat cttctcaacc aaagtggtct
     6841 gcggtgtaat cggatatgcc gatacgacat gaggccttga aagctttact ccgtaagccg
     6901 ctgcctcatt ggcggacaaa aaagcttttt tactcattcc gtatcaccgt ccttaatcat
     6961 tttaatgcag tttttcgggc attcggctgc gcagataccg catcctttgc agtaatccat
     7021 atctatttca agatggtcac cgctcttgtc tatgcagcct tccgggcaaa atacccagca
     7081 catcatgcat tttatgcacg ctttactgtc gatttccgga tgcaatgtcc tgaaacccga
     7141 atttggagtc ggcagtatcc ctaccccatt gcttgatcca aaaaacattt catcccagga
     7201 ggatggtgct ttataatcct tgttccattt cggtttgttc attattgcat caccgcctcc
     7261 ttaaccttgt cataagctgc ccttgccgct ttcttgttct tttccctgag attcctgccc
     7321 attacatcgt caatggcttt ctcaaccgat tccattgata ccatttcggt tgcgcctact
     7381 gccgcaccga gcatgattgt atttactatg ggattcccca aaagttcaag ggcaatatcc
     7441 gtggcgtcaa gaacaactat tctggatgga tcctcaaatc cgatatgtcg tgccgcttca
     7501 tccgctgttt ttcttgtgtt gataaccagg acaccgtcgg gcttaagtcc cgcagccaca
     7561 tttgtcacat cacacaaagt ctcatccagc acaattacgc agtcacattc ataaacctgg
     7621 ctgtggtcgg taatggtttt gtccgctatt ctggtaaaac caagcaccgg tgctccgcgg
     7681 cgctcagggc cgaaggatgg aaacgcctgt gccgatttac cttcgaatac cgatgctgca
     7741 acgcccagaa gcctggcggc tgtaaaggct ccattgcccc cccggccatg ccatcttatc
     7801 tgtttcactt tttcttttcc tccttcaatg ggctctctac ctgagccagg gcacgtgcat
     7861 aatctacata attgtcccat ttagtcgctg ccttcactac catctttgcc gtgtcaggag
     7921 acccgccgcc gtgcatacag cccggcactc ctcctcctat tgtcagccac tctacaagtc
     7981 tagccatctt aatcctgtct tctccaggta ctgcagacct taatgattct ataagcttct
     8041 ctccgtataa cggtgacatc atatccttat aggaaggcat gcatcctgtc tctgttatgc
     8101 ctcctgagat ttcctgggcc aataccttcg tatcgtatgg caaggttgcc acatgcacct
     8161 tgtttacatg ggcaagcagc ggattgggat aaaatgctcc cgatttgtgc tttttccctg
     8221 tatacatggc tcctattccc agtccgtatg ttgtttcatt gtttattgcc atcttggtaa
     8281 gcttgtcctg gaatgccttc tgtgcaagcc cgtttgctct tgccattcct aatgccgctc
     8341 ctatcatcac gtctccctgc cctgctacac atgctcctat ggctgccctg tatatcgcag
     8401 taaagttgcc aatcagcttc cctgtatatt ttgtttctcc gttcataaac acatgcttgc
     8461 tgggtacgaa tacattgtca aatatgatat aggcctgggt ggttccggac tttataccgt
     8521 cccagccttc ctcttctatt cttgtatccg aaggacgcct tgtctctaca atagtcaccc
     8581 cttccgcatc tctgggcact gcggcggcta ttgcgtagtc accctcatcc tctcccattc
     8641 cggtggtagg catgaccatt atatactgtg acccaactgt tccgcatatc tggttcttga
     8701 agcctcggat tacgacccca tcttctcttc tttccactac atgcaggtac atgtcgggat
     8761 tatcctgctg acttggctta agtgccctgt ttccctttgc atcagtcagt gccccgctta
     8821 atgcaagagc attattctct gcgaatatgc agaagttctt taatctttca tgataatccg
     8881 ttccaaattc ctggtccatg tcaaaggtta cactccaaag tgcgttcaat accgtccatc
     8941 ctgcacatac aggccccata cagcttccgc aggtatgata tccgtctctt ttcatgtttg
     9001 cgttccccag catgtcatct gcgcttgtta tcagtgtatt ccagcgatgg gccttgttgc
     9061 ctgaaagaaa tgaagtattt gtataaagct gttccttttc cgggtcgaag gaattgtcgt
     9121 accattttgc tacattcctt atatgggatg ctgtggcagg atgttctgtc acatcctcta
     9181 taagttctcc ccacttgtat acattgggcc tcatcttctt catttcctca atatactgtg
     9241 ctcctgtttt taatgccatt gtataccctc ctttataaat caagtttaat catcaactga
     9301 tccagaatcc tgttcaggtc tgcggcggca cctgcgtccg atacctcaaa tactgccgcc
     9361 ttttcatcct tgttgatgct tattatcaat ccggaatcct tcattccgca tatatgatgg
     9421 gtcgccccgg atatcccgaa gcctacatat gccttcggcc ttatggactt gccgctggtg
     9481 cctaccatta catgttctcc cggtgcccat ccctcatcca ctactgggcg ggtgcaggct
     9541 gccgctcctc cgaatatcgc cgccagcttc tcaagcttcc cccagttctc tttgcagcca
     9601 atgccgtagc ctccgcagaa tactacttcc gctttgtcca gaggcactcc ttctgtttct
     9661 ttcttctcaa ggcttattgc ctttaagggc agattatccg ctgtaattgc gctgacatcc
     9721 acttttataa tttgggcttc cctttcttcc ggttctccct gtacaaaaat tccgggcttg
     9781 acgcttgcca tctggggccg tgtgtcggga cacagtatct ctcccagtat cttccctccg
     9841 aaggacggca ctgcggctac cagatgttca ttttcatcta tatgcatatc tacacagtgg
     9901 gcggctactc ccgttttcag ctgtgccgct gctgtaggcc ccagttccga gcctattgcc
     9961 gtggctccga aaaggaatat gtcagggtct tctttttgca ccagttctgt tattataggt
    10021 gcatacagca tcgggttata taagctcagt tcgtcggatt ccaccaggta tattttttct
    10081 gctccgtatt ccgccagtgt tttgcatgtg cttcctatcc tgctgcctat aagtaccgct
    10141 gccacttccg tttctccctt gagtcccgat gccagctcct ttgcttttgt caaaagctcc
    10201 agggtgactc tgtccagggt gcagttcaca tattcggcaa atacccaaat ttttttcatt
    10261 gccgggcacc tccctctatg atgcatgcat caccttttgt ggggatgttt actcctgctg
    10321 tcctgagttt ttctatgatg gcatccgcta tttcttctgc tgaaccggta agcatttctc
    10381 cttttcgctt gagatccggt gtgaatatgg ctcccgcttt tgtgggtgag ccggcaagtc
    10441 ccagtctttc ttcttctgcg tcaaggtctg ctttacccca tgttgttatc ggcttgtttt
    10501 tggcttttat gattcccatt gccgatatga accggggttt gttcagttct ctggatactc
    10561 ccagcaggca gggcaattta acttcccatt ccatgaagcc gttttctgct ttggttctga
    10621 ctctgagggt tctttcctcc aggttttcca ggctgaatac attccacaaa tggggtactc
    10681 caagccattc tcccaattgt gaggatacct gggctgtagc tccgtctgcg ctctctgtgc
    10741 cgcagaatac aatgtcgaag ccttccagct tttctattcc ctttgccagg gtgtaggagg
    10801 tagccagggt atctgctcct ccaaaggctc tgtccgacag cagtactgct tcgtccgctc
    10861 ccattgccag tgcttccttg agattctccg tggcatttgg aggggccatg gtgagtactg
    10921 ttactttgcc cccgtactgt gctttaagct tcagggctgc ttctatccca ttcttgtcta
    10981 cggggtttat tattgtcggt attccttccc tggttattct ttttgttacg gggtctatgc
    11041 ttactttgtc atagtaatca ggatccggca ccggcttgat gcatactact atttttaaag
    11101 ccataagtat tctcctttat ctgtttctgc tctgcttagc tctcttatgc tttgaatgcc
    11161 gatagtgtat ttcccgctat gataagctgc tgtatgtggg aggttcctcc tgaaggtatt
    11221 gatgccagag catcccttaa gaaacgtcct acagggtatt cgtttattac tccatagcct
    11281 cccataaggt ccatggttct ttttgatgct cttaccgccg cttctgtgga gtagtatttt
    11341 gctattgcaa aatccggtgt tgcagggagt tttgcatcct ttttccctgc tgcatagtaa
    11401 agaagcattt ttgccgcttc gtattcggtg cgtatgtttg ctatttcaaa ttgaattgcc
    11461 tgaagctttg ctatcggctt tccgtaaact actctttcgt tggcaaattt gactgcttct
    11521 tctacgcagc ctcttaaaat gcctacgcag atagcactca ttcccgctct tcctacttct
    11581 cctattgcgg ccatgcctat ctttgctccg gctccgacat tgcctatgat gttctcttga
    11641 ggtatcttaa cttcgttcag tatcacgtct cctgtaaccg agcctctgag tcccatttta
    11701 tgctctttgc gtccggcacc aaaacccggt gtaccctttt ctattatgaa tgcactcagc
    11761 tggttccttc ctttttcgtc ctggcctgtt actgcagtaa tcacgtttac gtctgcatga
    11821 tggttggtga tgaagcattt acgaccgttt agcacccact gtccgtcaac taattctgct
    11881 gtggttttct gccctgcaaa gtccgagcct ccgccgggtt ctgtgactgc aagcccgcat
    11941 agttttgttc cggcacataa ctcagggaga tatttcttct tctgctcttc agtcccgaaa
    12001 tccaatattg gcgacattcc aagatggtgt gtcatgaggg caatgcccaa tcctgcggaa
    12061 tacctcgata tttcctctat gcatattgct ctttccacat gccccatccc tgctccgccg
    12121 tactcttcag gggcgaatat tccgttgatt cccaattcgc ccattatggg aaaaagctcc
    12181 attgggcaat agtcattttc atcccattct gccgctttag gcgccacttc cgcttctgca
    12241 aactcccgca ccgctttgcg aagtaattcc tgatcttcgg taaattcaaa aaattgcata
    12301 tacactatct ccttgttaat tttttaacta tataaatcat atatagtttc ctaaaaatat
    12361 gatagcatta cgaattttgt ttgtcaacaa attactttta tacagcattc ccttaaacca
    12421 agtaattcca atgccttatc aggggtacac tccttaagcc ctcatataac tattacggtt
    12481 tttttattaa aggtccattg acaaatttac ttaatttctt tattctttat gttattataa
    12541 atatttaaat ccggaagaag gttgtattgt gatgatgctg gacgaatgta tcaacttttt
    12601 gctgactaaa acccaacagg tagtatttca gcaatttaag gcaggcttgg ccaaatatga
    12661 cgtaaccccc gcacaatacg gagtattgaa atgcctatgg tctcaggact gtcagaatcc
    12721 cagccagatt gcctgcagct tatttctgga cgcttcaacc attaccggaa tccttgaccg
    12781 gatggagaac aaggacatga taaaaagaat agcagatccc aaggacaaga gatccttaaa
    12841 ggtagtgctg acagagcggg gaaggctgct ggagaaaccg gtgacggaaa tcattgatga
    12901 agagaacaaa aaggtgctgc atgctctgaa tgaaggggag cggaagaagc ttgcagagta
    12961 tttgaaccgg ataagctcca tataggtttt ttgaggaatg atgcttatat gatatatgat
    13021 gaaccattat tcaggccccc cagtgaggct tacagcttga tttttcaagc tacaataggg
    13081 tgctcccata ataaatgctc cttctgcggc atgtataaaa gcaagaaatt caggatccgc
    13141 agcaagagcg atgtattcag ggacctggag gaaatggctg cacgctataa taaggtgcgc
    13201 aggatttttc tcgcagacgg tgatgctttg gtgctgaaga cccgggacct taaagatatt
    13261 ctgcaaaaaa tcagagaact tttcccctgt tgcgaacggg tgggaatata tgccactccc
    13321 ggggatatat tgaggaaatc ccctgaggat ttgacgtccc ttaaagagca tggcctcggg
    13381 atagtatatc tgggagttga gtccggcagt gataaaatcc ttaaggctgt agataaggga
    13441 gcggactcca aagaaataat tgaagcggga aagcgcatag taagctcggg tatcaagctt
    13501 tccgtaaccc ttatttccgg gttgggggga aaggatggct gggaggagca tgcaagagaa
    13561 tccgcaaggg ttattaacga gataaaccct gactatctgg gtttgctgac cttgctgatt
    13621 gagcccggca cccggatgca tgatttggtg cagcaaggca ccttcaagct gctgtccgct
    13681 gaagaagtag cccttgagac tcaactgctg atccggaatc ttgagcttcg gaactgtatt
    13741 ttcagaagca accatgcctc caattacttt tcccttgaag gaacccttcc ccatgacaaa
    13801 agcaaactgc tgaagcaact ggataatatt ctgaacaaca ttgaagatta cagcggagag
    13861 tacttcagga gattatgaag cagcggtgaa tcagctccta acggttcttt ttcgcaagga
    13921 tgcgcagctt ttcaaatcta ttttcattcc ccgcaataag cctcttaata ttgcttctgt
    13981 gagtgtagaa tatgaaaact gcaataataa tgctgaaaat aagggctgtc tgatcgatat
    14041 tatccgtatt attaatatat aaggctgtca ttgtcacagg aaacacaatt gctgctgtca
    14101 aagagcttaa ggatacatat ccgcttgtca gcagcacaac agcaaatgaa gctgccgaaa
    14161 ccaggagggg cacgggaaac aaagcaataa gcatccccgt tgatgcagcg atgccctttc
    14221 cgcctttgaa acctgcaaat atggtgaagc aatgtcctat taccgcagca gcacctgcga
    14281 gtatcggaac cagcatactg ctttggggcg cccctccgga aagattcagt cgcgatatcc
    14341 actctgttgc cgcaaaaccc ttgaaaatat ctatgaacac aacaaaaatg ccggttttcc
    14401 agcccataac cctgaataca tttgtagctc ccgcgttccc gcttccatgt tttctgatat
    14461 caattccctt tacagctttg catactatta tgcttgttgg tatggaacct gcgagatagc
    14521 tgagaataat tatgactgca atatttatca tgctattata caatgtgctc attattatcc
    14581 tcccaatcct tatttttcaa atgacaatat tgtatctgcg tgctttcagc gccaccgtct
    14641 tgtgtgttat ccccaaggct tttcccgcgg cgttataact gccaaacctt gccagagcct
    14701 ctttaattat ctctttttca tattcctcca gagtagctat attacccgaa ttattcatat
    14761 taataagtgc aggggcggaa catgtatgcc ggtttacgca ataaagcgac gatattttgg
    14821 aaggcaggct gtttatgtca atttcattcc cgtcagtaag agctatgact ctctccacca
    14881 ggttttccaa ttcccttata ttaccgggcc aatcgtaatt cagcaggcag ttttcagcct
    14941 ccggggatac tccggttatt tccttattca tatttatgct tattcttttt ataaaatact
    15001 ccaaaagcat aggtatatct tccctgcgtt cttttaggga aggaacataa ataggcagta
    15061 catttaatct gtaatacagg tcttccctga aggctccctt ctctaccaat gcctcaagat
    15121 tacaatgtgt tgctgcaata attcttacat cacaccttat tgtatcacaa ccgccgattc
    15181 tttcaaattc cttatcctgt ataactctta aaatctttac ctgcatatcc ggcggcatat
    15241 caccaatttc atccaaaaat aatgttccgc cgtcagcctg ttcaaatttt cctgtttttc
    15301 tcctgacagc cccggtaaaa gcaccccgct catagccgaa cagttccgat tccaagagag
    15361 tagacggtat agctccgcaa ttcactattg aataggggcc ctttgccctg ctgctgttat
    15421 tatgtatggc tctggctatc agcccctttc ctgttccgct ttctccgcaa atcaacacat
    15481 ttgagcttgt ctgagccgct ttgtaagcta tcttaagcgc tttcataatt gaactgcttt
    15541 ctccgactat atctttgaac atagttctta tattattcat gtaattaacg ggcggtgaat
    15601 tgaattctat tacagtatct tttttcctgc aaatatcctg tgcagcttct tccctgtaaa
    15661 tagctactac tcccttaaat tcaccgtttg aatatatggg agaagcggag gcgcttacat
    15721 gagcagcacc cttataaagg acaagatgtc cgcatataca tcttttctcc ctgaatgcag
    15781 ttaagagtac atcgtcattc tttgtcagaa aaatattcat ccccgtttct atgcggtcac
    15841 ttatctttag tattctttcg taagcgccgt taaagtattc gatatagccc cgctcattta
    15901 ttatacagac tccgtcagta tggtcgtcaa atatataaaa gctttcggca cctaaaaaac
    15961 cgtcctttgc tgccttaccg gctttgagac ttaagctggc aactctgttg ttcttcacat
    16021 tcaaccctcc cttcattact aaatttggtt aaacagttct tgaagaaatc acatttgcgc
    16081 cggtggaaag gatattttcg attataactg ctccaagtgc tattggagta tctacctcca
    16141 ctgaatttat tactttcata caatcaagca gtaaattctt tggcacaggt ttatccgtcc
    16201 ttaccggcaa tctcctcaaa atagcattat tcagctttac tgttgtggtt aatattctgg
    16261 cgggattagt aacttccgca atggcatacc caatacccct attgcatctg ttccctttga
    16321 ccaggtaacc cgaatcactg ctgctatccc caattacctc caagctgcag cctataggac
    16381 aaatggtgca tgtcacaata ttcctctcca tatacatcct ccctcactca acatctacgg
    16441 ttaacgataa atcctccgct gtcttcaagc tttcagctgt aatgcatata cgttccattt
    16501 ctcccggtcg catatatgac ttcttaatta ttttgattac agtgtctccg aattttacca
    16561 ccagtttttt attcgtgaat attttgttca cacgcatgaa cagatcaaca gattgctcaa
    16621 ctttatcctt cctgataaaa tggggaacta tatacctgat tccattgccc ggttttgtcc
    16681 ttatggcagg accgtacccg cctgtttcac catttacaaa gcgggcagca tttcttcccg
    16741 ctatccggct ttcctcagtc acataatcaa caagatcatg gacatgaact gcgtttccgc
    16801 aagcgaatac tcccttaacc gttgtttcca tggattcatt tacgacaggg ccgccggtta
    16861 tgggatccag ctccacgtcc gcctctttgg aaagttcgtt ttcgggaatc aagcccactg
    16921 ataacagaag ggtatcacac tcataaaact gctctgttcc tttaatgggt ttcctgtttt
    16981 catcaagctc tgctaccgta accccttcta ttctgtcctt tcctttaata tcaacaattg
    17041 tatgatttag gagcagcgga atgccgtagt cctccaggca ctgaacaaca ttccttatca
    17101 gccctcccgg gtaaggcaga gcttcggcaa ccactttgat ccgggcccct tcaagggtaa
    17161 gccttcttgc cataatcata ccgatatccc ctgaaccaag tatcactaca tttctgccta
    17221 ccatatagcc ctctttattg acaaaccgtt gtgccgtacc cgccgtaaat atgccgcttg
    17281 gccttgttcc cggtatattt attgcccctc tggttctttc cctgcagccc atagccaata
    17341 ttgccgccct tgccttaata ttgacaaacc cctctgtttt attgaccatg ctgataacct
    17401 tctcttcggt tatgtttagg gccattgtgt ccagtttgta ttctattccc agctccttga
    17461 attcttttat gaaccgttcg gcatattccg tcccggtcaa ttcctccctg aatatatgaa
    17521 gcccgaatcc gttgtgcaca cattgctgca gtatgcctcc cagttccttt tccctatcaa
    17581 tgagcaatac tgttcctcca tgtttttttg cttctattgc tgccgcaagt cctgccggcc
    17641 cgccgccgat tacagcaata tcatactcat tcataacaac ccctccctaa actttccctg
    17701 atgctgcagc aaggtcgtga acatcctcat ataagctgtt atccttggtt tctcctgtca
    17761 atatatatga tttggggctg ttttttacaa cgtctttgat atcaatgtcc aactcccttg
    17821 caagtatttc catcactctc ggcccgcaaa agcttccctg gcaccgcccc gaacccggcc
    17881 ttaccctttt cttcacgcca tccagggacc tggcacctgc ctttctgtga attgcatcaa
    17941 caatttcccc ttccgttatg ctttcacacc tgcatattat cctcccatat ctgggatcct
    18001 gctttataag caaattttta tcctcctggg aaagctccat aaaccttaca acctttcttc
    18061 ttacaggatt aaacccgaga ttttcctcaa gccctcccat tgatcccttt actatatccg
    18121 ctgcatgtac ggcaattgcg ggagccgctg tcagtcccgg cgattcaatc cctgcgacat
    18181 ttataaaccc ctttgcctcc ttggattcac ctatgacaaa atcatctgtg cttgatcttg
    18241 ccctgattcc cgcaaaggcc gtaatcactg cgttgggctt caggcctttc gctgaccttg
    18301 ccgatgcctc ccatatatat gagatgcctt ccggtgttgt ctgtatagct tccttatcct
    18361 ctatatcctc cgcatccgga ccgattaaaa tattcccatg gacggtcgga gctatgagaa
    18421 ctcccttgcc aaaatccgag gggcattgga atataactgt atttacaaag tttcccaggc
    18481 ttttgtcaaa caagttgtac tgccctcttc tgggcagtat tttaaagctt ggagcagcta
    18541 ccatttcgtt aaccttgtct gaatacagcc ctgcacaatt aattacaaat cttgcttctg
    18601 cgagtctgtc tcccatgtgc agcctgtaca ggccattcat tttctcaatt ttggttactt
    18661 cactgtccaa aaaaagctcc actccgtttt ctactgcatt ctccgccaga gctatagcca
    18721 attcccacgg cgaaactatg ccggcattcc gggcatacaa tgccccgact acttcatcgc
    18781 ttaaattacc atctatctcc tgtactttat ccttaccgat tatctccata tcaggcaccc
    18841 cattagcgat gccctggtca taaagctttt gtattgtctt catttcctcc gggctgaaag
    18901 ccaaaacaag ggatccggtc tttttataag ggacatcaag ctcactgcac agcttttcat
    18961 acattaaagc ccccggaaca ttaaaccttg ctttgagagt tcccggcttt gcgtcatatc
    19021 cggcatggac tattgcactg tttgcctttg ttgtgccgtc tgcaacatca ctattctttt
    19081 caatcaaaag gattctgaca ttgtaccttg ataattccct tgcaatagaa cagcctgtaa
    19141 ttcccgcacc gataacagct atatcataca tttcagttcc tccaatacca gtaccttgca
    19201 gcacctaata gctgcatgat acttgtctgt ttgttccact acagcgttgc tgtcaatcgc
    19261 cgtagtcacc gctacgcctc tttccagcgc cttgcattgg aagcaaatat cctgcgtata
    19321 ataatgcagc aatcagatgt ctcaggtact aagaattctg ataaaataaa gccgcaccag
    19381 tctacaccta tcatcccata ggttagcttg tgcggctctc caaatcaaaa gccctcaata
    19441 tttacattaa attctatgct attacatcat tagaatatac caaaatggca taaataagat
    19501 tattcttccc acttaagggt tctgctgaca gcctttttcc atccggcata ctttttatcc
    19561 cttgaactgc tatccataac aggctcaaat accctgtcaa cattccactt ttcggctatt
    19621 tcatccttgc tgtcccagaa tccgacagca aggcctgcaa ggtatgcagc accgagtgct
    19681 gtggtttcgg ttacttcagg cctgtaaatc ggtacaccga gtatatctgc ctggaattgc
    19741 atcaggaagt tgtttgcaac agcgccgccg tcaaccttca gtgcctggag attgatcttt
    19801 gagtcttcct gcattgcttc aagcacgtct ctggtctgat aagcaattga ctccaatgtt
    19861 gctcttacta tatggtttct gttggctcct ctggtcagtc ccaaaatgct ccctcttgcg
    19921 tacatatccc aatagggggc acccaatcct acgaaagcgg gaacaacata tactccgttg
    19981 gtatccgcaa ctgcggctgc catatcttcg cttgccgcag cattgtcaat tatcttcaac
    20041 tcatctctaa gccactgaac tgatgcaccg gctataaaga tgctgccttc cagagcatat
    20101 tccactttgc cattcaagcc ccacgctatg gttgtcagca ggccattact tgatgaaact
    20161 gccttctccc ctgtattcat cagcatgaag cagcctgttc catatgtatt cttagccata
    20221 ccgggcttat agcatgcctg accgaataat gccgcctgct ggtctccggc cgcccctgct
    20281 atcggtattt cagcgccgaa gatttccttg tcggtttctc cgtatacaaa gcttgaagac
    20341 tttgcttccg gaagcattgc ttcgggaata tccagttcct ccagtatttc cttatcccaa
    20401 gccaattcat gtatattaaa cagcatagtc cttgaagcat tggagtaatc tgttacatgt
    20461 acctttccct ttgtcaggtt ccatatcagc catgtatcta tgtttccgaa gcacagctcc
    20521 ccattttttg cccgctctct ggctccttca acattatcaa gtatccattt taccttggtg
    20581 cctgaaaaat atgcatccac aacaagtcct gtcttattcc ttattgtttc agccagcccc
    20641 tttgccttaa gctcatcgca tataggtgct gtcctcctgc actgccatac tattgcattg
    20701 tatatgggct ttccggtact tctttcccat actacggtag tttctctctg gttggtgata
    20761 cctatggcag ctatatcgtc agctccgatg cctattttgt ccatggcctc tcttgcaaca
    20821 ccgctctggg taccccagat ttccatagga tcatgttcaa cccagccggc ctttggatat
    20881 atctgagtga actccttttg cgccacgctg actatcttgc cgtcattacc gaatataatc
    20941 gctctcgagc ttgtggtgcc ctggtctaat gccagtatat attttgacat atgtgttccc
    21001 cctttatttt aattaaataa ctttacagag gtagttatat tatatcatta ccggaataat
    21061 cccatataca aaaatgtgcc tattatcgcg ccgacaatcg gcccaaatac cggaacccag
    21121 gaataactcc aatcatttcc gcctttcccg gctatgggca gtatggcatg ggctatacga
    21181 ggcccaaggt cacggcaagg attgattgcg tatccggtag gcccgccaag cgaacagcct
    21241 attgccgtaa tcattgctgc ggttaccata ggcccaagtc cgtctgcaag cttattggca
    21301 ccaatagcca ggcacataaa tgccaggaca gcagtaacta tcatttccgt caggaaattc
    21361 ttcccatagt cccttacagc aggagccgta cagaacacac ccagctttgc tgccttatct
    21421 tccgttatcg cccagtgcgg caaatatatc agccatacta ctgctgcacc gattatagct
    21481 ccaagcatct gaccggctac tattcccggt accaatgccc aatccaatgt tccggctaca
    21541 gcattagcta tggtaagagc cggattaaat gctgttcccg gtgctgccaa agcagccatg
    21601 cctacagcaa ggccccagcc taccgctata acaatccagc ccgatgattg tgacttggac
    21661 ttgttcagtg taactgccgc acatacgcct ccgccaaata tgatgagaat cattgcacct
    21721 atgagttcac ttaaaaacgg tgacataata tttacccctt ccttcattat aatctcctgt
    21781 ccattttcca gagttgatat ttctgacccc ccttctccct tattttctcc gtcgctgcca
    21841 tgacgtgaat aataacggcc ttacatgtac caaagctcct ttttactggt ggataccccc
    21901 agtgcaccgg cattcaggca atcaattaca tcctctttat ctcttatcaa gccccccgct
    21961 attaccggaa tacttgtttg accatgaacc tcattaatta ccttcggcat tattccgggt
    22021 aagatctcta tagcatccgg ttttgttgta tgcaaggatt ctattccgct tgccagtgac
    22081 agcgaatcca gtgcgaagag cctctggatt atgaatatgc cgttgttttt tgctattcta
    22141 accaggttgc tttttgtggt aataattcca tccggcttta tattgtcatg aaaatatctt
    22201 aaggacacag catcttttga aaaaccttct ataaggtcca tgtgtacaaa aacaccctta
    22261 ccggcgcttt taaacctgtc aacaatacct gctatattcg aaatgtctcc cttcagcagg
    22321 aaaacaatat tgcagggtga ttctgcggca agctcgatat tgttcaaatc atttattgct
    22381 gcaattattg ggttcttata aattaatgga aagaatttat ttgacatagg aatgcctcct
    22441 cgtcttatgt attatagcaa caggcatgcc aaaggaaaaa agcgctgttt tactagtttt
    22501 attagtgata tgtggggctt agttgataaa tattaccagg atttttattg tgatgataaa
    22561 atatattgat agaatgtacc aaatagaatt gt
//
LOCUS       DAOQUR010000035        22412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2709_length_22412_cov_9.542604, whole genome shotgun sequence.
ACCESSION   DAOQUR010000035 DAOQUR010000000
VERSION     DAOQUR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2709_length_22412_cov_9.542604"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..5078
                     /locus_tag="PKI50_08285"
     CDS             153..5078
                     /locus_tag="PKI50_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HNT03735.1"
                     /translation="MNKEYVIRLYNDICNRLLRLDLPDNSKDAAKKYIEDVSFIENLH
                     KIVRNRDYSCNSVLLLCQSLMNQVAEDNIPSNWLEYVYQYTLSKSFPHAVEAELKNGL
                     EGSCSIYLQTLREISEFQKNGSDNTWQSRYPIRFLSSEEENELENPIEYKKFLKAFDE
                     LYVYEMMKLNQEVLGFNTLDHICGVHYLALYIGRELKKAGIPIDLGRVSGAAAGHDIG
                     KYGCKSSESKRVPYLHYYYTDLWFRNHDITYIGHIATNHSTWDLELENLPLESLVLIY
                     SDFRVKHDATGNTREMHIYPLKDSFNIILDKLDNLDAIKEKKYRRVYSKLADFEDYLI
                     SLGIEVDINSISKGIRRELGIKNRHYSLMSGNQVIKSIKHLAISHNINLMYQLRDEAS
                     LNRILESARSEANWKKLREYLRIFEEYSTYLTQKQKLITIRFLYEQLVHPEDDIRRQC
                     AELIGLLIARFDEKYRKDVPENAILDTPVMTSFELLDKYMQLLVSPDHKIVPLHKSYL
                     GYSISIIVSSLFSHCGKSQTEGFRTVAAKYYKEGARRNNETKLYLLEALRYMPVCGDH
                     EADRIICSYVKRRLSDKSNAIRLSAMDVAYNILARSRKNTDTSGDNTAIGSLALSVKE
                     MISGTHRRSGVVSENYLKHKIACLLEMEPAVLNKFHEYCHKDLHKLSEISLSNLKTAT
                     EWVIKRIQLDLLLDNSLSNPKSNGFYSAMHFCNLMKVSASESVRNRAGEALTHIVSHL
                     SPEQRNDIAVEMLRALEIEGYQFAEYIPNYLGKIILSLEPVELDEVLDDFKEKIKNST
                     PHLDSLILKTIGITIANYPSYIKTFKEKREVFENRLIRILSIIINGLAHYDADVKQSA
                     FSVLGKEIFGSSTLMPEEKNEIFIRIAKKLLTLLEDDIREGLLFLTNSAGLINIYRFI
                     SDYTFFYKDIEIAVPEKVALFPGAFDPFSLGHKEIARAIRDMGFEVYLAVDEFSWSKK
                     TLPNLIRKSIINMSVAEEFNIYIYPEDYPTNIANPLDLEVLRNNFPASEVYIVAGSDV
                     MINASAYKKEISKNSVLTFSHIIFERKSSYNQYEKDAGKLSETIGKIDGKVIRLSLPP
                     QYEDISSTQIRSNVDENRDISMLVDPLAQKFIYENGFYQSEPQYKSMVQTESIEIEVV
                     EKASRELFNELSSSLCIDKESLADSLGKFLGKIASRLLLIRDADHKRQILGFSAFHRI
                     SSSFLYQEFGNSSVTDYIRENSTGKIVSIDGLFILNNTVLENMEQILLAETLAYCIAR
                     DYDYAVFHSTLSPISYDMLEALRLMGFRDLPCPGCLNKVLAVNISTPCALNLDIETII
                     KEPYVSSSSFKDIVSRTRKRLQSTLAEVYAGNLILPFDRAVMDSTLVKKICEVNVVPT
                     KPLKPRHLGPAMCVPYGNILSRSIIPNTVTKALHTERLYGPDAKDYKVGSFPYYLDLE
                     IQMKMLKSFDRPIILVDDTLHHGFRLKTLDPMLKKENIEIRKIIVGILSGKGKELMEI
                     QGNDTDCAYFIPRLREWYNENNFYPFIGGDALWRGIYPKRNLLPSINLILPYTSPSFL
                     NETSKKSIYMLSHTAMENAIEILTTLEDEFQAINERSLTLSSMGQVFITPRCPDHGKN
                     MDYDLSLSPSFYLKNDLELLDRLERIICNIF"
     gene            5209..>5474
                     /locus_tag="PKI50_08290"
     CDS             5209..>5474
                     /locus_tag="PKI50_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HNT03736.1"
                     /translation="MMFYYKLFNKTLVSRKKYLQLEEIPEEEAASGAGRIFVLDSCPP
                     GESRRSFCASQPDLVYNLKEGLDMLLLPDSEDNNLPDWIIKAVSG"
     assembly_gap    5475..5574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5575..5904
                     /locus_tag="PKI50_08295"
     CDS             <5575..5904
                     /locus_tag="PKI50_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HNT03737.1"
                     /translation="GEGLVIADSIKNYNDRLSRYLTEKSKTANLEVRKLGYKPYIAPA
                     LSSGALSILATIRGQWHYSATFLGGVYMGARNRLNASGIELERLPVPEELFLRLKNTY
                     ESLRKMP"
     gene            5901..6950
                     /locus_tag="PKI50_08300"
     CDS             5901..6950
                     /locus_tag="PKI50_08300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNT03738.1"
                     /translation="MSDYYVIIPGNASLQLKKMLEAVLTGKNASYITDKQSLPDLRGV
                     KIIVCAEISPAGLCIPLYEILEELQSRGCDSLKDSSAILLLHSSNELYTKSTASLIVF
                     LMNQLGCRFPGHPAVEATGSLLNFSAWRKIKKASLEDICLELCTSLGERFAAYNPAAV
                     KKPKILALHSSRKETSNTLALWNMVKNSLEGCRISELHVENGSVVDCKGCSYKTCKHF
                     SMNNSCFYGGIMVKEILPAIEKSDAVIWLCPNYNDAVSANLSAVINRMSALYVKTPFY
                     NKSLFSIIVSGNSGSDSVARQLLGALCINKGFNLPPYHSFMATANDPGTALRIPGIEA
                     AAKKFSENIVNELKI"
     gene            7159..8412
                     /locus_tag="PKI50_08305"
     CDS             7159..8412
                     /locus_tag="PKI50_08305"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNT03739.1"
                     /translation="MAHQDNLNPFLNAQKQVKTACDKLGLDPKVYEILKQPMKVLEVA
                     IPVKMDDGSLRIFTGYRAQHNDAVGPTKGGIRFHQNVNRDEVCALSIWMTFKCCVTGI
                     PYGGGKGGIIVDPTTLSQGELERLSRGYIQAIYKLIGEKIDVPAPDVGTNGQIMAWMV
                     DEYNKLVGRSELGVITGKPVEFGGSLGRNAATGFGVAVVAREAAKKVGIDFKGASIAV
                     QGLGNVGSHTALNLQKLGGKIVAVCEWNGSIYDEKGLDINALVAYMKQNKKIVGFPGS
                     KEITKAEFFALPVDILVPAALENQITAEVAETIRAKLVCEAANGPTTPDGDEVINKKG
                     LLLTPDILTNAGGVTVSYFEWVQNLYGYYWTEKEVEEKEERAMVEAFNSIYAIKEEYN
                     VSMREAAYMFSIKKVAAAMKVRGWL"
     gene            8558..9091
                     /locus_tag="PKI50_08310"
     CDS             8558..9091
                     /locus_tag="PKI50_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNT03740.1"
                     /translation="MQRKLTVKDLSYISLFATLISVSGYIIIPLPFSAVPVTAQTLAV
                     MLAGSLLPVGQTVLSLLVFILMGAAGLPVFSGGSAGMGAILGKTGGYLIGFVAGAALI
                     SALKGKKPGFVRLAAANVLGGIVVVYSFGVFWLNYVTGIGISQAVIFGALPFIPGDIL
                     KVIIASMLSLRLSKHMK"
     gene            complement(9222..9560)
                     /locus_tag="PKI50_08315"
     CDS             complement(9222..9560)
                     /locus_tag="PKI50_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNT03741.1"
                     /translation="MLYNETKKIIVVDEIKIAGSFKERLKGLMFKSRIGKNEGLLMLG
                     CNSIHTCFMRFAIDVVFMDLNNRVIFIKEKVKPWRSIGFIKKAYMTLELPEGTVKNKN
                     IAIGDILILS"
     gene            complement(9574..10485)
                     /locus_tag="PKI50_08320"
     CDS             complement(9574..10485)
                     /locus_tag="PKI50_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT03742.1"
                     /translation="MLGAIMISTFSTVCFTMVMLLRIVFREKLHMQKRIKEYVFIDEK
                     SENILEGSFADRVIKPVMEKGNRFLGRIAPKHYYNYLERMINEAGNPKGITVGGVVLL
                     QIFSILVSAGAAVIMLLSTDIPTPKMLVLVCILLVLINIVPKFYLRHKISERQKEIER
                     GLPDVLDLLTVSIEAGLSFDGAVAKLVEKMSGVLAEEFSIVLKEMKVGVSKKDAFKSL
                     VERVPVPSLVTFVSSIIQADQLGVSIGNVLRIQSSQVRQKRMERTKENAMKAPVKMLL
                     PMIIFIMPTIFIVILGPVLIKIMEAFG"
     gene            complement(10488..11453)
                     /locus_tag="PKI50_08325"
     CDS             complement(10488..11453)
                     /locus_tag="PKI50_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT03743.1"
                     /translation="MPEILLPMLFINMTLVIYLLYKYFSGKRSVAVRMKQYLNPEKNR
                     EKPEKERGLPWRESLFVFSKTLRKSKFGIKYREKIEARLVQAHLPIKPEEYMSINVIV
                     FLASALVFFVFTENLAGSAVFALLAARVPGIYVNSKRRSIIHKIDQQLPDTLALISNT
                     LKSGYSFLQAIDTASRELPPPISLEFQRILKEINLGTNTEKAMESLGKRVQSQDLELI
                     IMAVLIQRQIGGNLSEILDNISDTIRSRIKIKGEIKVLTAQGRISGIIISLMPVVLGV
                     ILYFINPSYISLLFKHPLGLAMLGTAVVMQGLGIYLIRKIVRIEV"
     gene            complement(11471..12790)
                     /locus_tag="PKI50_08330"
     CDS             complement(11471..12790)
                     /locus_tag="PKI50_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNT03744.1"
                     /translation="MGLLKRIEQEKTEILSEGRQSIKETKKEDPLSDVKGIIHKKIIE
                     RIDTSMLGQEGEEQRFNAEIADIVEETLKEKELHLSRTERQTMIRYIIDESIGFGPIS
                     GHLLDEEVSEIMVNGPDQVYVEKKGRLELADTIFEDNQHVMRIIEKIVSPLGRRIDES
                     SPMVDARLPDGSRVNAIIPPLALNGPTITIRKFAKNPLNVGNLISYGTMTPEMALFIE
                     ACIKSRLNIIVSGGTGSGKTTTLNVLSSFIPHDERIVTIEDAAELQLEQEHVVRLESR
                     PANIEGKGAISIRDLVKNSLRMRPDRIVIGEVRGGEALDMLQAMNTGHDGSLTTGHSN
                     SPRDMLSRLETMVLMAGMDLPVRAIREQIASAIDIVIHQARLKDGSRKITHITEVQGM
                     EGDTIILQDIFVLNEKGTFTATGIRPKFMEKLANTRVSLPNNLFSQV"
     gene            complement(12899..14092)
                     /locus_tag="PKI50_08335"
     CDS             complement(12899..14092)
                     /locus_tag="PKI50_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT03745.1"
                     /translation="MANRIKVVIVDDSEQTRDNIASIISFEKDIEIVGEAENGEEAIA
                     VVRSKKPNIVLMDINMPLLDGIKATQILNEEGADASIIIMSIQGEGEYLRRAMQAGAR
                     DYVVKPFGVNDLVKTIRHVYDLDMKRRQKSNVASVIKVDSRILSVFSTKGGVGKTTVA
                     TNLAVALSIVTGKKIALLDFDLQFGDVAICLNLYVKNSMTDLVKDYSNIQQDPGLLEE
                     YLLTHYSGIKILAAPTRPENAEYVTPEHIRNTIEFLKGRYDYIIIDTSHGFSDNAITA
                     LDMSDTIIYLSALDLPSIKNTKNGLEVMKSLNYPKEKVKIVINKSNERFGINHNDFEE
                     ALGVEIWAMIPDDQASVTISVNKGHPLISHRRSSPVSKKIYKLAQSIASGEEKAKKKL
                     FAAAF"
     gene            complement(14092..14829)
                     /gene="cpaB"
                     /locus_tag="PKI50_08340"
     CDS             complement(14092..14829)
                     /gene="cpaB"
                     /locus_tag="PKI50_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNT03746.1"
                     /translation="MEGLNKKILALAIIMAFVTCMLLYFYISGLSSDSEIEYIEAYAA
                     NVEIPARTVVEDKMIVKVQVPKGTRIASGISDKSKIVGRLTKERIIKDELILPDRLYS
                     DEKTNMAFIIPTGKRAVTIGVNEVTEVGDFIIPGDYVDVIATFEEANVEIGGSKIYYP
                     KYTKIILQNVQVLGVGQNMQVLKEKDKDLPASVTLAVTPGEAERLVLADESGVLRLAL
                     KPAADNDTIQTNGVIKDDMVLPKGRVN"
     gene            complement(14854..>15633)
                     /locus_tag="PKI50_08345"
     CDS             complement(14854..>15633)
                     /locus_tag="PKI50_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03747.1"
                     /translation="VDSVALAAVSVYEEGEEKMLEEAHKYSNLNGVPPEELNIDISED
                     RKRVTVTTDKTVALYFARIFDRDSADVGVSAAAVAGSIVSAKGIRPFAVEQQEFEYGE
                     TYTLKEGAGGGHSGNYGALALGGNGASRYRNNLKNGYNASPPLRIGDEIETEPGNMAG
                     PTQDGIQEILDSDPNEHSEDLSQLEANCPRLIVIPVVDSLDVHGRSTATIVGFAAFFL
                     DDVVRNGGHTEITGRFIKKIGEGEIDESSEGYGLFGIKLVE"
     assembly_gap    15634..15733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15734..16026)
                     /locus_tag="PKI50_08350"
     CDS             complement(<15734..16026)
                     /locus_tag="PKI50_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNT03748.1"
                     /translation="MKLDKKGQSMVETALILPIIILLFLGMFEFSRIFGSYLLITHAS
                     REGARLAAIGRTDAEIQESIEGKVSILDESELQVILDPEEDDRITGEDVRVRV"
     gene            complement(16135..16320)
                     /locus_tag="PKI50_08355"
     CDS             complement(16135..16320)
                     /locus_tag="PKI50_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNT03749.1"
                     /translation="MMRLYNFWYDIKETLKDKKGQGMVEYALIIGLIAVVLITVLTNM
                     GTGIEDKFEEITNALTP"
     gene            complement(16603..17547)
                     /locus_tag="PKI50_08360"
     CDS             complement(16603..17547)
                     /locus_tag="PKI50_08360"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HNT03750.1"
                     /translation="MKRGDILLSDKEYIRHSLETNLFFLRIVKEHEIFAAASLPPRDR
                     GVMNQLVFMKNSTEQLLSEAIDLADRAVSHEVLASDELVTDYTLAAERATQFLTGISI
                     NTDLTRRELELRGSSKGRSAEGLQREISVLNRKAMAMTKAAVAFKTKLLKNVSECRAF
                     SYTYPTMLEHVIEEAEYYIMLLDKLEKRDGIDSIKEIVQEEINWNHIMEEHSKFIRGY
                     LDPEEEKLFDAANTFAKEFDRLLGKAEKAADNPELLPEVTEESIKNVIGLRNFKEKGT
                     EGILECRIKSVIPPLLADHVLREANHYLRLLKAFKEKL"
     gene            complement(17609..18106)
                     /gene="nrdG"
                     /locus_tag="PKI50_08365"
     CDS             complement(17609..18106)
                     /gene="nrdG"
                     /locus_tag="PKI50_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNT03751.1"
                     /translation="MELRVAGIVKESVVDGPGFRYVIFSQGCERRCKGCHNPDTHAMD
                     GGYVVETESIIEDIRQSRYIDGVTFSGGEPFLQTEAFVDIAEKLGEDNINIICYTGFT
                     YEELLGSKDESKIRLLGLIDTLIDGPYVEELKDLGLPYRGSKNQRMINVKSSLKQQEI
                     MLVNI"
     gene            complement(18118..20139)
                     /gene="nrdD"
                     /locus_tag="PKI50_08370"
     CDS             complement(18118..20139)
                     /gene="nrdD"
                     /locus_tag="PKI50_08370"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNT03752.1"
                     /translation="MFTKIRKRDGRVVDFTREKITGAIFKAARQVGGEDYETAENLTE
                     RVISYLYKHIKDEIPDVETVQDAVEKVLIETGHAKTAKAYILYRSKRTRIRETKSELM
                     DVVKDILIETSRDNANVGNSPSAKMLQIASAASKHYYLNNVIPEEMSKAHISGDIHIH
                     DLDYYGKTLNCLQIDLTKLLTDGFNTGYGFIRPPKRIGSAAAQAAIILQSNQNDMFGG
                     QSFPHFDKSMGEVIRNQCPNTDDDEIFQAMEGLIYNLNTMHSRAGAQVPFSSVNLGTD
                     TTPEGRKVTSAILTAFEKGLGKGESPIFPNLVFRLKEGVNMNAGDPNYDLFQFAMKVA
                     SRRMNPTFSFMDASFNSRFGDEVSYMGCRSRVIANRHGAEKSARRGNITPVTINLPKL
                     ALRTRDVDTFFVELNNILDLCSRQLIHRFNVISSLKVKDLPFLMGQKLYMGSENLKEQ
                     DEIREAIKNGTLAIGFIGLAEALTVLTGYHHGEDEESWKLGYQIVEGIKKAADNYSNE
                     YNLNFVAYATPAEGTSGRFIEKDRKEFGVIKGVTDKAYYTNSFHIPVNYQISAFEKIE
                     KEAPFHKLCAAGHISYVEMPSSPKDNLQAIEKIVKHMAECDIGYAGINFPIDECRSCG
                     VSGIIDSNCMRCGSEDIRRIRRITGYLSTIDRFNDAKQAELADRVKHAY"
     gene            complement(20151..20606)
                     /gene="nrdR"
                     /locus_tag="PKI50_08375"
     CDS             complement(20151..20606)
                     /gene="nrdR"
                     /locus_tag="PKI50_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNT03753.1"
                     /translation="MKCPYCGYSESKVVDSRPTVEEAAIRRRRECEKCSKRFTTYEKI
                     EEMPLIIVKKDGSREAYQRSKMLNGILRACEKRPISISTIEGMADEIEKELYNSMDKE
                     IESKKIGEMVMSKLKQIDDVAYVRFASVYRQFKDINIFLEELNKLIDER"
     gene            complement(21057..21287)
                     /locus_tag="PKI50_08380"
     CDS             complement(21057..21287)
                     /locus_tag="PKI50_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HNT03754.1"
                     /translation="MVKISELKQKDVININDGRRLGVVYDVEIDMEKGKIDALVVPGT
                     GKILGLFSKESDIVVNWGNIKKIGADVILIDG"
     gene            complement(21404..22174)
                     /gene="sigG"
                     /locus_tag="PKI50_08385"
     CDS             complement(21404..22174)
                     /gene="sigG"
                     /locus_tag="PKI50_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HNT03755.1"
                     /translation="MLNKVEICGVNTSKLPVLSNEKMVELLKKMQQGDQSSREEFIKG
                     NLRLVLSVIQRFNNRGEYVDDLFQVGCIGLIKAIDNFDLGQNVKFSTYAVPMIIGEIR
                     RYLRDNNSIRVSRSLRDIAYKALQVRDMLIGKNSKEPTVSEIANELKLPVEEVVFALD
                     AIQDPVSLFEPIYHDSGDAIYVMDQVSDDRNTDDNWLESIALKEAMKKLNDREKLILN
                     LRFFEGRTQMEVAEEIGISQAQVSRLEKTALGHMRKHI"
BASE COUNT         6044 a         5257 c         4306 g         6605 t
ORIGIN      
        1 tccccgcgtc cccatgtccc cgtcgcaatt tccttcgttt tcgcattatt ataccacaat
       61 ctaatgaagt ttgaaaaata tatttaattg gaatataata taatttaact atgctgtagt
      121 ttccgcaatt tccagtgctt aaggaggatt ttatgaataa ggaatatgta atcaggctct
      181 acaatgatat atgcaatagg cttctccggc tggatttgcc tgataattcg aaggatgcag
      241 ctaaaaagta tatagaagac gtcagcttta ttgagaatct tcacaagatt gtcaggaaca
      301 gggattacag ctgcaattcc gttctgctgc tgtgccaaag ccttatgaac caagttgcag
      361 aggataatat tccctcaaat tggcttgaat atgtatatca gtataccctg agcaaatcct
      421 tcccccatgc cgttgaagcg gagcttaaga atggattgga aggctcatgc agcatatatc
      481 tgcagactct cagagagatt tccgaattcc aaaaaaacgg ctccgacaat acatggcaga
      541 gcagatatcc catacgattc ctatcttctg aagaagaaaa cgaattggaa aacccaattg
      601 aatataagaa attccttaag gcctttgatg aactgtatgt atatgaaatg atgaagctga
      661 atcaggaggt cttgggcttt aatacccttg accatatctg cggggtgcat tacctggctt
      721 tatatatagg aagggaactg aaaaaagccg gaatcccaat tgaccttggc agggtttcgg
      781 gagccgcagc tggccatgac attggaaaat acggatgcaa atcttctgaa tccaagaggg
      841 tgccgtatct tcattattac tataccgatt tgtggttcag aaatcatgac attacctata
      901 ttggccacat agccacaaac cattccacct gggatcttga gcttgaaaac cttccccttg
      961 agtccctggt actcatatac tcagacttca gagtaaaaca tgatgcaacc ggaaacacaa
     1021 gagaaatgca tatctatccc ctgaaggatt cctttaatat tattctggat aaactggata
     1081 atctggatgc cataaaggaa aagaagtaca gacgagtata ttccaagctt gcggactttg
     1141 aggattattt gataagcctt ggtatagaag ttgatataaa tagcatcagc aagggcataa
     1201 gaagggaatt gggaataaaa aaccgtcatt attccctgat gagcgggaac caggtaatca
     1261 aaagtatcaa gcacctggca atcagccata acataaatct tatgtatcag cttagggatg
     1321 aagcttccct taaccgcata ctggaatcag ccagaagtga agccaactgg aaaaagcttc
     1381 gggagtattt aagaattttt gaagaatact ctacctatct tacacagaaa caaaaactca
     1441 ttactatcag attcctttat gaacaactgg tgcaccccga ggatgatatc agaaggcaat
     1501 gtgcagagct gataggacta ttgatagcaa gattcgatga aaaatatcgc aaagatgtgc
     1561 ccgaaaacgc catacttgac actcccgtaa tgacaagctt cgagttgctg gacaaatata
     1621 tgcagcttct ggtcagccct gaccataaaa tagtacctct ccacaaaagt tacctggggt
     1681 acagcatcag tatcatagta tcttcattgt tttcacactg cggcaagagc cagacagaag
     1741 gcttcagaac tgttgccgcc aaatactata aggaaggcgc ccgcagaaac aatgagacaa
     1801 agctgtatct gctcgaagcc ttaaggtata tgcctgtctg cggtgaccat gaagcagaca
     1861 ggataatctg cagctatgtc aaaaggcggc ttagcgataa aagcaatgca atcaggcttt
     1921 ccgcaatgga cgtggcatat aatatccttg cccgttcccg gaaaaatacc gatacatccg
     1981 gagataatac cgcgatcggc agcctggcac tctctgtcaa ggaaatgata tcaggcaccc
     2041 atagaagatc cggcgttgtt tccgaaaact atctgaaaca taaaatcgca tgcttgctgg
     2101 aaatggagcc ggcggtactg aataaattcc atgagtactg ccataaggac cttcacaagc
     2161 tttcagaaat ctcattgagc aaccttaaaa ccgcaacaga atgggtaata aaaagaattc
     2221 agctggatct gcttctggac aattcattaa gtaatccaaa atccaacggg ttttattcag
     2281 ctatgcattt ttgcaatctt atgaaggtca gtgcctccga aagtgtccgc aatcgtgccg
     2341 gagaggctct gacacatata gtttcccact tgtccccgga gcagaggaat gacattgccg
     2401 tggaaatgct ccgtgccctt gaaattgaag gataccagtt cgcagaatac attccgaact
     2461 acctgggaaa aattatacta tcccttgagc ccgtggaact tgatgaggtc cttgacgatt
     2521 tcaaggagaa gataaagaac tccacccccc acttggactc tctgatatta aaaaccatag
     2581 gcattacaat agcaaattat ccatcctata tcaaaacatt taaagagaaa agggaagtct
     2641 tcgaaaacag gctcataagg atactcagta tcataatcaa cgggcttgcc cattatgacg
     2701 cagatgtaaa acagtctgcc ttcagcgttt taggcaagga gatcttcggt tcaagtactc
     2761 tgatgccgga agaaaagaac gagattttta tccgtattgc caagaagctg ctcacactgc
     2821 ttgaggacga tatccgggaa gggctgttgt tccttacgaa ttcagcaggc ttaatcaata
     2881 tttacaggtt tatttccgat tatactttct tttacaaaga tattgaaata gctgtccctg
     2941 agaaggttgc acttttcccg ggggcttttg atcctttctc tctggggcac aaggagattg
     3001 cgagagcaat acgtgatatg ggctttgaag tttacctggc cgtggacgaa ttttcctggt
     3061 caaagaaaac cctgcctaat cttatcagga aaagcattat taatatgtcc gttgccgaag
     3121 aattcaacat atacatctat cccgaggatt atccaacaaa tattgcgaat cctttggacc
     3181 ttgaggtact gaggaacaat ttcccggcgt cggaggttta tatagttgcg ggaagcgatg
     3241 taatgattaa tgcctcggca tataaaaaag aaataagcaa aaactccgta ttaacttttt
     3301 cacatataat atttgaaagg aagagttcct ataatcaata tgaaaaagat gcaggcaagc
     3361 tttcggaaac aatcggcaaa atcgacggaa aggtaatccg cctctctctt cctcctcaat
     3421 atgaggatat cagttctacc cagataagaa gcaatgttga tgaaaacaga gatatatcaa
     3481 tgctggtgga tcctctggcc cagaaattca tctatgaaaa cggattctat cagagtgagc
     3541 cccaatataa gtcaatggta cagacagagt ccatcgaaat cgaagtagtg gagaaagcaa
     3601 gcagagagct gttcaatgaa ttatcctcat ccctgtgtat cgacaaagaa tcccttgcag
     3661 acagcctagg aaagtttctg ggaaaaatag cctcaaggct tctattaatc agagatgcgg
     3721 atcataagag gcaaatcctt ggtttttctg cttttcacag aatcagctcc agcttccttt
     3781 accaggaatt cgggaacagt tccgtaactg attatataag agaaaactcc acaggaaaaa
     3841 tagtgagtat tgacggttta ttcatattga ataacaccgt tctggaaaac atggaacaga
     3901 ttcttctggc ggaaacactg gcttactgta tagcccggga ttatgactac gcagtattcc
     3961 acagcacatt aagccccata tcctacgaca tgcttgaagc cctcaggctg atgggcttca
     4021 gggatctgcc ctgtcccgga tgcctgaaca aagtgctggc agtaaatata agcaccccct
     4081 gcgccttgaa cctggatata gaaaccataa tcaaggaacc atacgtaagc agcagctctt
     4141 ttaaggatat agtctcaaga accagaaaga ggcttcaaag cactcttgct gaagtatatg
     4201 cgggaaatct gatactgccc tttgacaggg cagtcatgga cagcactctg gttaaaaaaa
     4261 tatgcgaggt gaatgtggtt cccacaaagc ctttgaagcc aaggcacctg gggcctgcta
     4321 tgtgcgtacc ctacggcaat atactcagca gaagcattat tcccaacaca gtaacaaagg
     4381 cacttcatac agaaaggcta tacggccctg atgctaaaga ttacaaggtc gggagtttcc
     4441 cgtattatct tgatcttgaa atccagatga agatgctgaa atcctttgac aggcccatta
     4501 ttctggtgga tgacaccctt catcacggct tcaggctgaa aaccctggac ccaatgctga
     4561 aaaaagagaa tattgagata aggaagataa tagtcggcat actttccgga aaagggaagg
     4621 aattaatgga aattcagggg aatgatacgg attgtgcata ttttattccg cgtctgagag
     4681 aatggtataa cgaaaacaac ttctatccct ttattggcgg agatgcttta tggaggggca
     4741 tatatccgaa aaggaacctg cttccctcaa taaacctgat actcccttat acctcaccct
     4801 cttttcttaa cgaaacatca aaaaaatcaa tatatatgct ttcgcatacc gccatggaaa
     4861 atgcgataga aatactgaca accttggagg atgaattcca ggccattaat gaaaggagcc
     4921 tgaccctgtc ttccatgggg caggtgttta taacacccag gtgtcccgat cacggcaaaa
     4981 acatggacta tgaccttagc ctgagcccat ccttctatct taaaaacgat ttggagctgt
     5041 tggacaggct cgagcgaatc atatgcaata tattttagga tatacatttg atacatgttt
     5101 cggatatcct aaaatatatt ccttgattca tagaatacgt caagactacc agttataatg
     5161 aatcggctgc acacaggcgt attctatatg aaatacatcg ggggtaatat gatgttttat
     5221 tataagctat tcaacaagac cctggtatcc cggaaaaaat acctgcagct ggaagaaatc
     5281 ccggaagagg aagcggcttc cggtgccggg cgtatatttg tgctggacag ctgccctccc
     5341 ggagaatcaa gaaggagctt ctgcgcttcc cagcctgatc ttgtttataa cttgaaggaa
     5401 ggccttgata tgcttcttct tcccgattca gaggataata acctgccgga ttggataatt
     5461 aaagctgtca gcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgaa
     5581 ggcctggtaa tagcggacag tataaaaaac tacaatgacc gtctttcccg ttatcttacc
     5641 gaaaaatcaa aaacagcaaa cctggaagtc cgcaagctgg gctataagcc ctatatcgct
     5701 cctgctctct cgtcaggcgc cttgtcaata cttgcaacaa taagaggaca gtggcattac
     5761 agcgctacct tcctgggggg agtatacatg ggagcaagaa acaggcttaa tgcttcagga
     5821 atagagcttg agaggcttcc tgttccggag gagctttttc taagattgaa aaatacatat
     5881 gaaagcctga ggaaaatgcc atgagcgatt attatgtgat aattcccgga aatgcttctc
     5941 tgcagctgaa aaaaatgctg gaagccgtat tgacgggaaa aaatgcttca tacataacag
     6001 acaagcaaag cttacccgat ctcaggggag taaagataat agtatgcgct gaaataagcc
     6061 ctgccggctt atgcataccc ttgtatgaaa tattggaaga gcttcaaagc cggggctgcg
     6121 attccctgaa ggattcctct gcaatactgc tgctgcacag ctcaaatgaa ctatatacaa
     6181 agagtactgc ttcccttata gtattcctga tgaatcaatt gggctgccgc ttccccggcc
     6241 atcctgccgt agaagccaca ggcagtctgt taaacttctc cgcctggaga aaaataaaga
     6301 aagcctccct tgaagatatc tgcttggagc tatgcacttc actgggagaa agattcgccg
     6361 catacaatcc cgcagcagtt aaaaaaccga aaatacttgc gcttcactcc agccggaagg
     6421 agacctcaaa tacacttgcc ctgtggaata tggtaaaaaa cagccttgaa ggatgcagaa
     6481 taagcgaact ccacgtggaa aacggcagtg tagttgattg taaagggtgt tcttataaaa
     6541 cctgcaagca tttcagcatg aacaacagct gcttttatgg cggcataatg gttaaggaaa
     6601 tactgcccgc catagaaaaa tctgatgcgg tcatatggct ttgccccaat tacaatgacg
     6661 cggtatccgc aaacctttcc gcagtgatta accgtatgtc ggcattatat gtaaaaacac
     6721 ctttctataa taaatcattg ttttccataa tcgtatccgg caattcaggc agtgattcgg
     6781 tagccaggca gctgctgggg gcactttgca taaacaaagg cttcaatctg cctccctatc
     6841 attcatttat ggctactgcc aacgatccgg ggacagcttt aagaataccc ggaatcgaag
     6901 cagcggcaaa gaaattttca gaaaatatcg taaatgagct aaaaatataa attgttaaaa
     6961 atttagcagg agttttgatt tttatataga aatgttgtat tatatatctg aatggattaa
     7021 tatgctatac tatttttttg gatgaaaatg tacatgccat aagaagtgat tgaatcagaa
     7081 ccggcttgat aatcagcaat tatgtttctc ataatttgct tattatatta ataattacaa
     7141 ttgggaggtt aataaaaaat ggcacatcag gacaatttga accctttctt gaatgctcag
     7201 aagcaggtaa aaacagcttg cgataagtta gggttggatc caaaagttta cgaaatctta
     7261 aaacaaccga tgaaggtact tgaagtagct atcccagtga aaatggatga cggttcactg
     7321 agaatcttta caggatacag agctcagcac aatgatgctg ttggtcctac aaagggtgga
     7381 atcagattcc atcagaacgt aaacagggat gaggtttgtg ccttatcaat ctggatgaca
     7441 tttaaatgct gcgttacagg tattccttac ggcggaggaa aaggcggaat tattgttgac
     7501 ccaaccacac tgtctcaggg agagcttgaa agattaagcc gaggatacat acaggcaata
     7561 tataaattaa taggcgaaaa aattgacgtt ccggcaccgg atgttggaac aaacggtcag
     7621 attatggcat ggatggttga cgaatataat aagctggtcg gccgcagtga attaggagta
     7681 atcacaggca aacccgttga attcggcggt tcattaggca gaaacgctgc tacaggcttt
     7741 ggtgttgctg ttgtagcaag agaagctgct aaaaaagtag gcattgactt caagggcgca
     7801 tcaattgccg tacagggctt aggcaatgtc ggaagccata ctgcattgaa tcttcagaaa
     7861 cttggcggaa aaattgttgc tgtttgcgaa tggaacggat ccatatatga cgaaaagggc
     7921 ctcgatatca acgctttggt agcttacatg aaacaaaaca agaagatcgt cggcttcccg
     7981 ggatcaaaag aaataacaaa ggcagagttc tttgcattac cggttgacat tcttgttcct
     8041 gccgctctgg aaaaccaaat tacagcagaa gttgcagaga caataagagc aaaactcgtc
     8101 tgtgaagctg caaacggacc tacaactcct gacggtgatg aagttatcaa taagaaggga
     8161 cttcttctga caccggacat attgaccaat gcaggcggag ttacagtatc atactttgaa
     8221 tgggtacaga acctgtatgg ctactactgg acagaaaaag aagttgaaga aaaagaagaa
     8281 agagcaatgg ttgaagcatt caattcaatt tatgcaataa aagaagaata caatgtttca
     8341 atgagagaag cagcttacat gttctcaatc aagaaggttg cagctgctat gaaggttagg
     8401 ggctggctgt aagaaaacaa ccggaggcct gtgaattatc acaggcctct tttatcggcc
     8461 aattgtcaac cttattattt ttgtggttga caattggcat tatcagtgtg tacaatggta
     8521 tatatagcat attcatataa tttttcggag gatacatatg caaagaaaac taacagtaaa
     8581 agacttgagt tacatatcgt tattcgccac actgatttct gtttccggat atattatcat
     8641 acctcttcct ttttccgcag ttcccgtaac tgcacaaacc ctggcggtaa tgcttgcggg
     8701 aagcctgctt cctgtcgggc aaactgtttt aagcctcctg gtttttattc ttatgggtgc
     8761 ggcaggcctt cccgtattct cagggggcag tgccggcatg ggcgctattt tgggcaagac
     8821 cggaggctac ctgataggct ttgttgccgg tgctgccctg atatcggccc tgaagggaaa
     8881 aaagccgggt tttgtacggc ttgcggcggc aaatgtattg ggtgggattg ttgtggtgta
     8941 ttcctttgga gtattttggc tgaattatgt cacaggcata ggaatttctc aggcggtcat
     9001 attcggcgca ctgcccttca ttcccggaga tattttgaaa gtaataattg catccatgct
     9061 ttccttaagg ctttccaagc atatgaaatg atgggacatg gggggtggga gagacacggg
     9121 ggagacacgg gggagacacg gggacgttta acgtgtccct tgagggacac gttaaacgtc
     9181 cccgtgtctc ccccgtgtct cccccgtgtc cccgtatccc atcagcttag gataagaata
     9241 tctcctattg cgatattttt attcttaacc gtaccttccg gcagctccag agtcatatat
     9301 gctttcttta taaaaccgat gctgcgccac ggttttactt tttcctttat aaatattacc
     9361 ctgttgttca ggtccatgaa aacaacatct atggcaaacc tcataaagca tgtgtgtatg
     9421 gaattgcacc ccagcataag aagcccttcg tttttgccta tccggctttt gaacataagc
     9481 cctttgaggc gctccttaaa gctcccggct attttgattt catcaactac aattattttt
     9541 tttgtttcat tgtacagcat aagtgcctcc cttctatccg aaggcttcca tgattttaat
     9601 gagaacaggc cccagaatca ctatgaatat agtaggcatt atgaatatta tcatgggaag
     9661 cagcattttt acaggcgcct tcatagcatt ttcctttgtt ctctccattc ttttctgcct
     9721 tacctgggag gattgtattc taagaacatt tcctatgctt acgcccagtt gatcagcttg
     9781 tatgattgag cttacaaaag taacaaggct tggcaccggc accctttcaa caagagattt
     9841 gaaggcatcc ttttttgata cccccacttt catttccttc agcactattg agaattcttc
     9901 cgcaagcact ccggacattt tttcaaccag ctttgcaact gccccgtcaa agctaagtcc
     9961 cgcctctatg gatacagtaa ggaggtccag cacatccgga aggccccttt ctatttcctt
    10021 ctggcgttca cttattttgt gccttagata aaatttagga acaatattta tgagcacaag
    10081 aagtatgcat accaggacaa gcatcttcgg cgtgggtata tccgtggaaa gcagcataat
    10141 cacagctgcc cctgccgaga caaggattga gaatatctga agaagtacaa ctcctcctac
    10201 ggttattccc ttcggattcc ctgcttcatt aatcattctc tccaaataat tataatagtg
    10261 tttgggtgca atcctgccca agaatctgtt gcccttttcc ataaccggct ttattacccg
    10321 gtctgcaaag gaaccttcaa gaatattctc tgatttttca tctataaata catactcctt
    10381 aattcttttc tgcatatgaa gcttttctct gaaaacaatc cttaaaagca ttaccattgt
    10441 gaaacatacg gttgaaaacg tacttatcat aatcgcccca agcattgcta cacctcaatt
    10501 cttacaattt ttcttataag gtatatgccc aaaccctgca tcacaacggc tgtacccaac
    10561 atggcgagac ccagggggtg cttgaataac agtgaaatat aagatggatt aatgaagtac
    10621 aatataactc caagaacgac aggcatcagt gatatgataa tgccggagat tctcccctgg
    10681 gctgtcagca ccttgatttc tccctttatt tttatccttg agcgaattgt atccgaaata
    10741 ttatccaata tttccgacag gttgccccct atttgcctct gtataagaac cgccattatt
    10801 attagctcca ggtcctggct ttggactctt ttgcccaggc tttccatagc tttttctgta
    10861 ttggttccca agttgatttc cttcaatatc ctctgaaatt caagggatat tgggggagga
    10921 agctccctgg aagccgtatc tattgcctga agaaagctgt atccagactt cagggtattt
    10981 gagataaggg ctaatgtatc cggcagctgc tggtctatct tatgtataat gcttcttctt
    11041 tttgaattca cataaattcc gggcacgcgg gctgccagca aagcaaaaac ggccgaacct
    11101 gcaaggtttt ccgtaaatac aaaaaagaca agggcagatg caaggaatac tataacattt
    11161 attgacatgt attcctccgg ctttatgggc agatgcgcct gaacaagcct ggcttcaatc
    11221 ttctccctgt acttgattcc aaacttgctc tttctcagag tcttactgaa aacaaacaag
    11281 ctttcccgcc agggcagccc tctttctttt tcaggttttt ccctgttttt ctcggggtta
    11341 aggtattgct tcattcttac ggctacgctt ctctttcccg agaaatattt atacagcaag
    11401 tatatgacaa gggtcatatt tataaagagc ataggcagca agatttccgg cataatgaat
    11461 ccctcttttc tcatacctga ctgaacaggt tgtttggcaa ggataccctt gtatttgcaa
    11521 gcttctccat gaatttgggc cttattcctg tcgcggtaaa ggtacccttt tcattcagca
    11581 caaatatgtc ctgaagtatt atggtatccc cctccattcc ttgaacttcc gtgatgtggg
    11641 ttatcttcct gcttccatcc ttaagccttg cctgatgtat tacgatgtca attgctgaag
    11701 ctatctgttc ccttatagct cttacaggca ggtccatgcc tgccatcagc accatggtct
    11761 caagacgtga aagcatatcc ctgggagaat tggagtggcc tgtggtaagt gaaccgtcat
    11821 ggcctgtatt cattgcctgc agcatatcca aggcttctcc ccctctgact tcgcctatta
    11881 ctatcctgtc aggcctcatc ctcagtgaat tctttacgag atccctgata ctgatggctc
    11941 cttttccctc aatatttgct ggcctgctct caagcctgac cacatgctcc tgctccagct
    12001 gcagctctgc tgcatcctct atggttacta tcctctcatc atgtggtatg aaggaggaaa
    12061 gtacattcag ggttgtagtt tttccgcttc cggtaccccc tgaaactatg atattaagcc
    12121 ttgacttgat acaagcttca ataaacagcg ccatttccgg ggtcattgta ccgtaagaaa
    12181 tcagattgcc cacattcaga ggattcttcg caaactttct tattgttatt gtcggcccgt
    12241 tcagagccaa aggcgggatt atggcattta ccctggagcc gtcaggaagg cgcgcatcca
    12301 ccatgggaga gctttcatct atccttcttc ccaaggggga cactattttt tctattatcc
    12361 tcatcacgtg ctgattatct tcaaatattg tatccgccag ttcaaggcgc ccttttttct
    12421 ccacatagac ctggtcaggc ccgttgacca ttatttccga aacttcctca tccagaagat
    12481 gcccgctgat ggggccgaag cctatggact cgtcaataat ataccttatc attgtctgcc
    12541 gttcggtacg ggaaagatgc agttcttttt ccttcagagt ttcctccaca atatcggcta
    12601 tttccgcatt gaacctctgc tcttcaccct cctgccccag catgcttgtg tcaattcttt
    12661 cgataatctt tttatggatt atccctttta catcacttaa gggatcctct ttttttgttt
    12721 ccttgatcga ttgtcttcct tccgacaata tttcggtttt ttcctgctct attcttttaa
    12781 gcaaacccat atttattacc tcctgggcat tcctatatag tctggtacga gacacgaggt
    12841 gcgaggtttg gtgttttctt cgagaacctg ctcctatcct cgtgccccgt gcccaatttt
    12901 aaaacgccgc cgcgaacagc ttcttcttag ccttttcctc accggatgct atactttgag
    12961 caagcttgta tatctttttt gacaccggtg acgaccttcg gtgagatata aggggatggc
    13021 ccttgttcac cgatatcgta acagatgcct gatcatccgg tatcatcgcc catatttcca
    13081 ccccaagggc ttcttcaaaa tcattatggt ttatgccgaa gcgttcattg gatttgttaa
    13141 tgacaatctt caccttctct ttggggtaat tcagggattt cattacctcc aggccatttt
    13201 tcgtattttt tattgacgga agatccaggg ctgataaata tataattgtg tccgacatat
    13261 ccagagcagt tatggcatta tcgctgaagc catgggaggt atcaattatt atataatcat
    13321 accttccctt aagaaattcg atggtgttcc tgatatgttc aggagtaaca tattctgcat
    13381 tttcagggcg tgtaggagcg gccagaatct ttatacctga ataatgggtg agaagatatt
    13441 cctccaataa gccgggatcc tgctgtatat ttgaataatc ctttacaagg tccgtcatgg
    13501 agttcttgac gtaaagattc aggcatattg ccacatctcc aaactgcagg tcaaaatcca
    13561 gcagtgctat cttttttccc gtaacaattg acagtgctac cgccagattg gttgctacgg
    13621 tagtcttccc taccccgccc tttgtagaaa atacgctcag tatcctggaa tctaccttga
    13681 ttaccgaggc aacgttactt ttctgcctcc ttttcatatc cagatcatat acatgcctta
    13741 ttgttttgac cagatcgttc actccgaagg gcttcactac ataatccctg gctcctgcct
    13801 gcatcgccct tcttaaatat tctccttcac cctgaattga cataataatt attgacgcat
    13861 ccgcaccctc ttcattaagt atttgtgtgg cttttatccc atccagcaaa ggcatgttga
    13921 tgtccatcag cacaatattt ggcttcttgc ttcttacgac cgctattgct tcctctccgt
    13981 tttcagcctc gccaacaatt tcaatatcct tttcaaagct gataatagac gcaatattgt
    14041 ccctggtctg ctcggaatca tcaacaataa caacttttat cctgtttgcc attaattcac
    14101 cctccctttg ggcaatacca tatcatcctt aataacacca ttggtctgaa tagtatcatt
    14161 atccgctgcc ggcttcaaag caagcctgag tacaccgctt tcatcagcaa gaacaagcct
    14221 ctccgcctca cccggagtta cagccaaagt cacgctggca ggaagatcct tgtctttttc
    14281 cttaagaacc tgcatattct ggcctactcc cagtacttgc acattctgca gtatgatctt
    14341 tgtatatttg ggatagtata tcttgcttcc gcctatttca acatttgctt cctcaaaggt
    14401 tgcaataaca tccacataat ccccggggat tataaaatcc cctacttcgg taacctcatt
    14461 cactcctatt gttacggcac gttttccggt aggtattata aaggccatat tggtcttttc
    14521 atcactataa agcctgtccg gaagtatcag ctcatcttta ataattcttt ccttcgtcag
    14581 ccttcccact atcttgctct tgtctgatat acccgatgca attcttgtac ccttcggaac
    14641 ctgcaccttc acaatcattt tatcttcgac taccgtcctg gcaggaattt caacgtttgc
    14701 cgcatatgct tcaatatatt cgatttccga atcgctgctc agccctgaaa tatagaaata
    14761 cagcagcatg cacgtaacaa aggccattat aattgccaga gcaagtattt ttttattcaa
    14821 accttccatt atataatccc cctctgtcaa acactactcc accagcttta tgccgaataa
    14881 tccgtatcct tcactgctct cgtcaatttc tccctctccg attttcttta tgaatcttcc
    14941 tgttatctct gtatgcccgc catttctaac cacatcatcc aaaaagaagg ccgcaaagcc
    15001 tacaatagta gccgtacttc ttccatgtac atccagggaa tcaacgacag gaatcactat
    15061 aagcctggga cagtttgcct caagctgtga aaggtcctca ctatgctcat tggggtcgga
    15121 atcaagtatt tcctgtatgc catcctgggt aggccccgcc atatttcccg gctcagtctc
    15181 aatctcatct cctattctta gaggcggact ggcgttatat ccatttttca gattgttcct
    15241 atatctactt gctccgtttc ccccaagagc caaagctcca tagttaccgc tgtgccctcc
    15301 tccggcacct tccttcaggg tgtaagtctc tccatattca aactcctgct gttctactgc
    15361 aaaagggcgg atacctttag ctgacactat agaacccgcc actgccgcgg cactaacccc
    15421 aacatccgca ctgtccctgt caaaaatcct cgcgaaatac agagcaacag tcttatcggt
    15481 tgtcacagtc accctttttc tgtcttcgga tatatctata ttcaattcct caggaggcac
    15541 accgttcaga ttggagtact tgtgcgcctc ctccagcatt ttttcttctc cctcttcata
    15601 gaccgaaaca gcggctaaag ccacagaatc aacnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnncttacgc gcacccttac atcctctccc gttatacggt catcctcctc
    15781 cgggtccaat attacctgca gctcgctttc atccaatata ctgaccttgc cttcaatgct
    15841 ttcctgtatc tcagcgtctg tccttcctat ggccgccagc cttgctccct cccttgacgc
    15901 atgagttata agaaggtagg agccaaatat ccttgaaaac tcaaacatac ccagaaaaag
    15961 caaaatgata ataggcaata tcagagccgt ctccaccatt gactggcctt ttttatcaag
    16021 cttcatggct tccctcctct tgtgaatagt aaattataga aagactgacc ccgcctggta
    16081 aaaggcaggg tctacaatcc attattatgt cacaaaaaac attcttatat cggtttatgg
    16141 agtaagtgca tttgtgatct cttcaaactt gtcctctatt ccggtaccca tatttgttag
    16201 aactgttata agcactactg cgatcaggcc gataatgagt gcatactcca ccataccctg
    16261 tccttttttg tcctttaaag tttcttttat gtcataccag aaattataaa gcctcatcat
    16321 cattggaact ccctccttca tataaatttt tatgtgcttt tattctactc ataattataa
    16381 cctgacacca tccgacaaat tattcatttt tcgttgcggc acaattgccc acgtcatgag
    16441 tactcttgct tatgctgaat atttatacaa gtcctattaa taaataaaag tactatgaaa
    16501 tttcatagta ctttttgatg atatcattat aggcaatatg gtcattgaac aggctataag
    16561 tcttagatag tcccatttcg cacaaggcac gcaaaaatga ttctaaagct tctccttaaa
    16621 ggctttcagc agcctaaggt aatgatttgc ttccctcagc acatgatctg ccagcagcgg
    16681 aggtatcacc gactttatcc tgcactccaa tatcccctct gtgccctttt ccttgaagtt
    16741 cctaagtcct ataacattct taatgctctc ctccgtaacc tccggaagaa gctccggatt
    16801 atcggcggct ttttctgcct ttcccagcag ccggtcaaat tccttggcaa aagtattggc
    16861 ggcatcgaac agcttctcct cttcaggatc caggtatcct ctgatgaact tgctgtgctc
    16921 ttccatgata tgattccagt ttatctcctc ttgaactatt tcctttatgc tgtctatacc
    16981 gtctctcttc tccagcttat caagcagcat gatataatac tccgcctctt ctattacatg
    17041 ctccagcatt gtaggatagg tataactgaa tgccctgcac tccgatacat ttttcaggag
    17101 ctttgtctta aaggcgacgg cagctttggt cattgccatt gcttttctgt taagcaccga
    17161 gatctccctt tgaagccctt cagcgcttct gcccttactt gaacctctca gctcaagctc
    17221 tcttcttgta agatcagtat ttatcgaaat gcctgtcaag aactgagttg ccctttccgc
    17281 agccagagta taatcagtaa ccagctcatc ggatgccagc acttcatggc ttacagccct
    17341 gtccgccagg tcaatggcct cggacagcag ctgctcggta ctgttcttca taaacaccaa
    17401 ttggttcatg accccccggt cccttggagg cagtgaagcc gctgcaaaaa tctcatgctc
    17461 cttaactatt cttaaaaaaa acagattcgt ttccagtgaa tgtctgatat actccttgtc
    17521 tgataacaaa atatcccctc ttttcattgc tatggccctg caatgcagta tcattaccat
    17581 agtattccgg agaggatata tgtgttactt atatattaac aagcataatc tcctgctgct
    17641 tcaaactgct ttttacatta atcattctct gattttttga gcctctgtaa ggaagcccca
    17701 aatctttcag ctcttccaca taaggcccgt caataagggt gtcaatgagc ccaagaagcc
    17761 tgatcttgct ctcatccttg cttcccaaaa gctcttcata tgtaaagccc gtatagcata
    17821 taatatttat attgtcttcc ccaagctttt ctgcaatatc aacgaaggcc tccgtctgaa
    17881 ggaagggttc tcctcctgag aaggtaactc cgtctatgta tctgctttgc ctgatatctt
    17941 caataatgct ttctgtttct accacatagc ctccgtccat cgcgtgagtg tcagggttgt
    18001 gacagccttt gcatctacgc tcacagcctt gggaaaatat cacgtatctg aatccggggc
    18061 cgtccactac gctttccttg acaataccgg ccactctcaa ttccataatg acgcctccta
    18121 atatgcatgt ttaaccctat ccgccaattc tgcctgttta gcatcgttga acctgtcaat
    18181 agtggacaag tagcctgtaa tcctcctgat tcttctgata tcttcacttc cgcacctcat
    18241 gcagttgctg tctattattc ccgacacgcc gcagcttctg cactcatcta ttggaaagtt
    18301 gatccctgca tagccaatat cacactctgc catgtgcttt acaatctttt caatggcctg
    18361 aagattgtcc ttcggtgaag aaggcatctc tacgtatgaa atatgccctg ccgcacacag
    18421 cttatggaag ggggcttctt tttcaatctt ttcaaatgcg cttatctgat aattgaccgg
    18481 gatatggaag gaattagtat aatatgcctt gtcggtaact ccctttatca ctccgaattc
    18541 tttcctgtcc ttctcaataa accttccgct ggttccttcc gccggggtag cataagctac
    18601 aaaattcagg ttatactcgt tggaatagtt gtctgcggct ttcttaatac cttccactat
    18661 ctgatagccc agcttccatg attcttcgtc ttccccgtgg tgatagcctg taagcactgt
    18721 aagcgcttcg gcaagtccta taaaacctat tgccagagta ccattcttta ttgcttctct
    18781 gatttcatcc tgctccttca ggttctcgga gcccatataa agcttttgtc ccatcaggaa
    18841 tggcaaatcc tttaccttga gagacgagat gacattgaat ctgtgaataa gctgcctgct
    18901 gcacaaatcc agaatgttat tcaattcaac aaagaaagta tcaacatctc ttgttctcaa
    18961 agccagcttg ggcaggttta ttgtaaccgg tgttatattt cctcttcttg ctgatttttc
    19021 agctccgtgc ctgtttgcaa taactcttga acgacagccc atgtaactga cctcatctcc
    19081 gaaacgcgag ttaaagctgg catccataaa gctgaaggta gggttcatcc ttctggatgc
    19141 aaccttcatg gcaaactgaa acaggtcata attgggatct ccggcattca tatttacccc
    19201 ttccttcaat ctgaatacca gattggggaa tatggggctt tcaccttttc ccaggccttt
    19261 ttcaaaggca gtcaagatcg cactggttac cttccttcct tcgggagtgg tatctgtgcc
    19321 gaggtttacg ctgctgaagg gcacctgggc tcccgccctg ctgtgcatcg tattcaggtt
    19381 gtatatcaag ccttccatag cctgaaatat ctcatcatca tccgtattgg ggcactggtt
    19441 ccttattacc tctcccatgc ttttgtcaaa atgggggaag ctctgccctc cgaacatgtc
    19501 attctgattg ctttgaagta ttattgcagc ctgcgcagct gcagagccta ttcttttcgg
    19561 aggcctgata aatccgtatc cggtattgaa accgtctgtc aaaagctttg tcagatctat
    19621 ctgaaggcag tttagagtct tgccatagta atccagatca tgtatgtgaa tatcacctga
    19681 aatatgcgcc ttgctcattt cttcaggtat gacgttgtta agataataat gcttgctggc
    19741 tgccgaggca atctgcagca tcttggcgga gggcgagttt ccgacattgg cattgtccct
    19801 gctggtttct atcagtatat ccttaactac gtccatcaac tcgctttttg tctctctgat
    19861 cctggttctc ttggatctgt ataatatgta cgctttggca gtcttggcat gccctgtctc
    19921 aataagaacc ttttccacag catcctgtac tgtttccaca tcaggaatct catccttaat
    19981 gtgcttataa agataggata ttactctttc tgtaagattc tctgcggtct cgtagtcttc
    20041 tccccctact tgccgcgctg ccttgaatat tgcacctgta atcttctccc tggtaaagtc
    20101 tactacccta ccatctcttt tgcgtatctt cgtgaacatt catctacccc ctatctctcg
    20161 tctattaatt tgttcagttc ctccaaaaaa atatttatat ccttgaattg tctgtatacc
    20221 gatgcaaacc gaacataagc aacatcatct atctgcttca gtttgctcat aaccatttct
    20281 ccgatttttt tgctttctat ctctttatcc attgaattgt aaagctcttt ttcgatttca
    20341 tccgccatcc cttcaatagt ggaaattgaa ataggcctct tctcgcaggc tcttaatatt
    20401 ccattcagca tcttgcttct ctggtaggct tctctgcttc cgtctttctt tacaataatc
    20461 aaaggcattt cttctatttt ttcataggtt gtaaatctct tggagcattt ttcgcattca
    20521 cgccttcttc ttattgccgc ttcctcaaca gtaggccttg aatccactac cttgctttca
    20581 ctgtagccgc aataagggca cttcacactg cttcctccct tctagtgcct cgtgaggcac
    20641 tgaatctatg tgatgattat tgtattaata atcactagta tatggaaaat tttgtcgcaa
    20701 taacagagct tgcaatacta tttccatgag tctcagttcc tatttttgca ccaatatatt
    20761 gtaccatata ctattatatt atacatgata tagtaaaatc acccttccca tttttccaaa
    20821 tttataaagt aatatatgtt atctccgtaa tcctttttat atctcttctt gtcataaaaa
    20881 tggaaaaaag cgactcacgt cgcttttttt cgctagggaa ccctatggtt cccttcgacg
    20941 gatgtaagca tcctgaaact cctttatcgg cagggggctt ctcccccttg catcccccat
    21001 cgtataggaa atatgctttc ctatacgata aaaacaggag actttctttt ctcccactaa
    21061 ccatctataa gaatcacatc agcaccgatt tttttaatat tcccccaatt gactactata
    21121 tcgctctctt tgctgaacag tcccagtatt tttcccgttc cgggaactac cagtgcatct
    21181 atctttcctt tctccatgtc aatttccacg tcgtaaacaa ctccgagcct tctgccgtca
    21241 tttatgttta ttacatcctt ctgtttaagt tccgatatct tgaccattca cacccctccc
    21301 atcatacttg tttgcttatg ctagtatata aagcggaagc aaaaaataga acaaaaaacc
    21361 tcaattgaac caaagcattc aaatgaggtt tctatgaatc atatcatatg tgttttctca
    21421 tatgtccaag ggctgtcttt tccagccggg agacctgagc ctgggagatt cctatttctt
    21481 ccgcaacctc catttgtgtc cttccctcaa aaaacctcag attaagtata agcttttccc
    21541 tgtcgttgag ctttttcatt gcttccttga gagcaatgct ttccagccaa ttatcgtcgg
    21601 tgttcctgtc atccgatact tgatccatta catatattgc atcaccgctg tcgtgataaa
    21661 taggttcaaa aagagatacc ggatcttgaa ttgcatccag cgcgaatacc acttcttcca
    21721 ccggaagttt aagctcattt gcaatttccg atacggtagg ttcctttgaa ttcttgccga
    21781 taagcatatc tcttacctga agggctttat aggcaatatc tctcaaggat ctgctgactc
    21841 tgatggagtt gttgtccctc aggtatcttc ttatttctcc aattatcatg ggtactgcat
    21901 aggtagaaaa tttgacgttc tgacccaggt caaaattgtc tattgcttta ataagaccta
    21961 tacagcctac ctggaaaagg tcatcaacat attcccctct gttgttaaat ctttgaatga
    22021 cgcttaaaac aagccttaga tttcctttta tgaattcttc ccttgaggat tggtccccct
    22081 gctgcatttt tttcagcagt tcaaccattt tttcattaga gagaacgggc agttttgaag
    22141 tatttacacc gcagatttct actttgttaa gcatagcaca agcacaccct tttcttaatg
    22201 ttgacatatt aattattacc ataggccaac tttattattc accgatgagc ttgtaccaat
    22261 ttgttttaaa cagaacaatt ttgtattaga agaataatgc gacagggaca ggagaaacgt
    22321 ccccgtgtcc tcacgtcgca ttcttcatac catcctgctg atttctttct ttagccgctt
    22381 tataatcctc ttctccagcc ttgatatgta tg
//
LOCUS       DAOQUR010000036        21954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2792_length_21954_cov_10.544180, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000036 DAOQUR010000000
VERSION     DAOQUR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21954
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_21954_cov_10.544180"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="PKI50_08390"
     CDS             complement(<1..104)
                     /locus_tag="PKI50_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNT03756.1"
                     /translation="MIITTTPNVEGRKVKEYKGIVFGEVISGVNFIKD"
     gene            complement(185..607)
                     /locus_tag="PKI50_08395"
     CDS             complement(185..607)
                     /locus_tag="PKI50_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT03757.1"
                     /translation="MNIKVMYHTINGNTGKVAEAMAEALGVKAERIGEGSIAEPVDML
                     FIGDGVYGGRPDNSTVDFIKTLNGSMVKKAAAFGTYGMQEKAIKIMKELLEEQGINVS
                     AESFGCRGKCWAIMNRRHPSAEELLAAKEFAEVELNKL"
     gene            complement(768..1874)
                     /locus_tag="PKI50_08400"
     CDS             complement(768..1874)
                     /locus_tag="PKI50_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HNT03758.1"
                     /translation="MNYRELDTPALLIDKDIMISNCRRMQDYANRCNVALRPHTKTHK
                     MSRLALLQEELGAKGIAVAKVGEAEVMAAKGLRDIFIANEIVGEEKLRRIRKLAETID
                     ISFGLDSIPQADMIEKAFEGSKKQAVVLIEIEVGEQRSGVIEEAAFVELVNHLKKCSN
                     IYLKGIFSHDGHSYAAENAETCKEIHLKAQERTLRFAQLAAEAGFHLETVSIGSTPSL
                     MHDFPILDGVTEIRPGTYIFMDASQGNAYGSLERNAATVLTTVISLPTPERVITDVGA
                     KGLTAQTRNKGFTATKGLGLIKGWPGVSIHAVYDEHAIIYNKEFHDSIRVGDKVEIIP
                     NHICPVVNLHETAYIVSNGEVIEEIPVDCRGKLK"
     gene            complement(1899..>2947)
                     /locus_tag="PKI50_08405"
     CDS             complement(1899..>2947)
                     /locus_tag="PKI50_08405"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT03759.1"
                     /translation="KKAKCDCGGLWKLDYKPPKFDMSLVDHDTWSIFRYRAFMPMEGA
                     HWREISLGEGITPVIRFNEDVLLKMDYFMPTLSFKDRGAAVLIAHCKAIGVDSVVQDS
                     SGNAGNSVAAYCAKAGIGCEIFVPEGTSPRKINMIKSHNAKVNVVPGTRDNCADVCRA
                     KVETEGKYYANHVYNPFFYEGTKTYIYEVYEQMERIPENLFVPLGNGTLFIGIVKALE
                     ELLESAVIDKMPKIIAVQSEHCDPLAKAVITGEKHPVKVIPQPTMAEGIAVGVPMRGK
                     EILEYTYKYKIDIITAPENRIQEARAALAAKGIYCEHTTAATYAAYLKYCELNGRISD
                     CLIPMCGAGLKSDH"
     assembly_gap    2948..3047
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3068..>4266
                     /locus_tag="PKI50_08410"
     CDS             3068..>4266
                     /locus_tag="PKI50_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT03760.1"
                     /translation="MENRYVNFNEKRMYIFSELVKRYWNGGLETAHDLTALTDYIKRK
                     YGFSDYELPFIKDHIRVAMGLDPKGDGTFTDELDIIKSFREIDRPVVARIESPCEHCG
                     KLDCECGKCKHTEAGKYETHMYSRKSEPIISNDKCLNCGYSAPDCDFGALADKIEFIP
                     VIDLLKDEKTPVFACAAPAIVGQFGEDVTMGMLRAALKMIGFTDMIEVAMFADILTIK
                     EAFEFNQLVKSEEDFFLTSCCCPVWFNMIKTNYPDIYKHLSPSVSPMIASGRILKKLY
                     NDAKVVFIAPCIAKKAEIKEPDLKGAIDYVLNFRELKEIFNALNINPGELPADEKDQA
                     SFGGRVYARTGGVSFSVKTVVNRLEPTRVIKLKAKKVDGVKECRRVLDDLRDGRKINA
                     NFIEGMG"
     assembly_gap    4267..4366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4367..6150)
                     /gene="glmS"
                     /locus_tag="PKI50_08415"
     CDS             complement(<4367..6150)
                     /gene="glmS"
                     /locus_tag="PKI50_08415"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNT03761.1"
                     /translation="MCGIVGYIGDKNAVPILMEGLRKLEYRGYDSAGVAVYTKENIEI
                     RKYKGRLSVLEEHLKKETLDGCLGIGHTRWATHGEPSDVNSHPHTNCSGDIAVVHNGI
                     IENYMGIKSMLIEKGYTFRSETDTEVLAHYVDYFYKTDLLEAVMKVLEKIRGSYAFAV
                     ISRREPDKIICVRKENPLIVGIGKGENFIASDIPAILNHTRRIYLLNENELAVIEKDR
                     ISFFDESGKEVGKEVFEVNWDVAAAEKGGFEHFMIKEIHEQPKAVKDTMTSRVLPDSA
                     GIKLDDIKLTKEELQNFNKIFMVACGTAYHACMVGKYVIEKLARIPVEVEIASEFRYR
                     DPIVDEKTLTIIVSQSGETLDTLAALKEARRKGSRILSIVNVVGSSIARESHDVLYTW
                     AGPEIAVASTKAYTTQLIAIYIIALYMAKLKGTLKDEEIESIKDEMRRLPGLVEETLK
                     YKSAIQKFATHNSNARDIFYLGRGIDYAVALEGSLKLKEISYIHSEAYAAGELKHGTI
                     ALIEKGTIVLAVLTQDELYEKMVSNVKEVKARGAFVFALAKEGSTEVENVADYTLYIP
                     KVSDILAPVVAVMPLQLLAYYMAVEKGC"
     gene            complement(6246..7595)
                     /gene="glmM"
                     /locus_tag="PKI50_08420"
     CDS             complement(6246..7595)
                     /gene="glmM"
                     /locus_tag="PKI50_08420"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNT03762.1"
                     /translation="MGRMFGTDGVRGIANKELSPALAFNLGRIGAYVLTEETHKKPRI
                     AVGKDTRISGDMLEAALIAGICSMGAEAVSLGVLPTPAVAYLTRHMGLDAGVVISASH
                     NPFEYNGIKFFNGEGYKLSDQLEDRIESLMLNPENGIAYPTGADIGKKVEVDNAVHGY
                     VDFLKKTIDVNLRGLKIAVDCANGASYIAAPSVLADLGAEIAVINNKPNGKNINVKCG
                     STHPEGLQKLVVESGAHIGLAFDGDADRLIAVDNRGNIVNGDHMLAIFARHLKGKGML
                     KKDTVVATVMSNMGLDIALKREKCNLVKTKVGDRYVLEEMVSMGYSIGGEQSGHIIFL
                     DHNTTGDGLLTALQLLAVVKETGKKLSDLASFMKELPQVLVNAKVKNENKDKYMKDEA
                     IAGMITGLEKKLKGRGRVLIRPSGTEPLIRVMLEGANETEIKEDAEALAKLIEEKLN"
     gene            complement(7696..9387)
                     /gene="argS"
                     /locus_tag="PKI50_08425"
     CDS             complement(7696..9387)
                     /gene="argS"
                     /locus_tag="PKI50_08425"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNT03763.1"
                     /translation="MNFKVEIAKKINTLVDMEFDKVMELIEIPPQTNMGDYAFPCFQL
                     ARVMRKSPNMIAAEIAGKVQADSIVEKAEAVGGYVNFFINKKEYIRIMIQEILEKGEK
                     FGSSEEGKDKNVIVEFSSVNVAKPFHVGHLFNTILGSALEKVFRHSGYNTIRINHLGD
                     WGTQFGKLISAHKRWINEEALEKEPISELFRIYVKFHEEAEKDPALEDEARAYFKKLE
                     EGDSEVTILWQRFKDLSLREFSEIYEMLNVEFDSYAGESFYSDKMDEVVQILREKKLL
                     KDSNGAKIVDLEQFGIPPIMILKSDGATIYATRDLAAAIYRKRTYDFYKNIYVVGGTQ
                     SLHFNQIFSTLGLMGFEWSEDCVHIGHGLVKFADKKLSTRKGDVVFAKDVMNEAVHKT
                     MEVIEHKNPNLENKEEVARKVGMGALLYTFLKNNREKDVVFTWEDALNFDGDSGPYVQ
                     YTYVRGRSILQKAGYVPDTADLTFLSTEDEFQLVKLLGAFKDSVKEAVERYEPFVVVR
                     HVTEIAKAYNKFYNTHPILNADENVKNARLQLTKAVGIVIKTGLSLAGIETPDNM"
     gene            complement(9496..10032)
                     /locus_tag="PKI50_08430"
     CDS             complement(9496..10032)
                     /locus_tag="PKI50_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT03764.1"
                     /translation="MAHEIIMAGFGGQGVMAMGKILVEAAMEEGKNVSWLPSYGPEMR
                     GGTANCSVIVSDEPIGAPIVTEATAAIVMNRPSLDKYENTVVPGGALLINSSLIDKKA
                     TREDIKVYYVPANEIANELGTDKVANIVMLGAYLQSSGAASFDSIMNIITHIFAGKKA
                     SVIPLNKEALLRGAACIK"
     gene            complement(10032..10778)
                     /locus_tag="PKI50_08435"
     CDS             complement(10032..10778)
                     /locus_tag="PKI50_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT03765.1"
                     /translation="MAVVFEKTRGLAEKPFHYCPGCTHGIIHRLVAESLDELNLIDDA
                     IGVAPVGCAVFAYDYFNCDMQEAAHGRAPAAATGIKRVNPNAAVFTYQGDGDLASIGA
                     AEVVHAAMRGEKITTIFVNNAIYGMTGGQMAPTTLVGQKATTAPYGRDENHAGMPLRV
                     SEMLATIPKAAFVERVSVHDVKHIIQAKKAIKKAFQLQMEGKGFTLVEVLSTCPTNWG
                     KTPIEALKWLQENMIPYYPLGNFKNIEEVK"
     gene            complement(10778..11845)
                     /locus_tag="PKI50_08440"
     CDS             complement(10778..11845)
                     /locus_tag="PKI50_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNT03766.1"
                     /translation="MAKELWKGNEAVAEAAIKAGCRFFFGYPITPQNEIPEYMAMKMP
                     QIDGCFLQAESEVAAINMVYGAAGAGARVMTSSSSPGISLKQEGITYIAGAELPCVIV
                     NMVRGGPGLGGIQPAQSDYFQSCKGGGHGDYRLVVLAPASVQEAVDLTQEAFDIADQY
                     RNPVLILGDGMIGQMMEPVEFTEVKKRKLPEKTWATVGTKGKRKTNVINSLFIDPADC
                     ERHNFHLFEKYAEIEKNEIKVEEYNLEGAEIVFAAYGTTARIVKNAINSLAKEGIKAG
                     LIRPITLWPFPAKAFEKAAEQAGVKAFMSVEMSMGQMVEDVKLSVNGKKPVYFYGRTG
                     GMFPTPKAIAEEAKKILGGAK"
     gene            complement(11865..12071)
                     /locus_tag="PKI50_08445"
     CDS             complement(11865..12071)
                     /locus_tag="PKI50_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT03767.1"
                     /translation="MAKVTFDENRCKGCELCTTVCPVKIVVMDKDRINIKGYHPATVK
                     EMDKCTGCASCARMCPDVVIKVER"
     gene            complement(12121..12795)
                     /locus_tag="PKI50_08450"
     CDS             complement(12121..12795)
                     /locus_tag="PKI50_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03768.1"
                     /translation="MYNDRRVRIITGHYGSGKTEFAVNYAIALAEAGKKTAIVDLDIV
                     NPYFRSREREPLLVEKGIRVISSSVKGIFGDLPALSPEIYSVLQDTSYEVVLDVGGDK
                     VGARALGRYHEYFDREPYDMFFVLNANRHQTSTVDGAISYLRSIEHGSMQKVTALVNN
                     THLCGNTTVEDIMRGQKLCEDVSKVLGLPIKYVVVEKKFVDVLPKGLRGDVLPINIYM
                     KKPWED"
     gene            complement(12823..13896)
                     /gene="buk"
                     /locus_tag="PKI50_08455"
     CDS             complement(12823..13896)
                     /gene="buk"
                     /locus_tag="PKI50_08455"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNT03769.1"
                     /translation="MQEYRLLIINPGSTSTKIAIFDNEKPVLEETLRHSNEELAPFGA
                     IIEQYEFRKNVILDTLNANGINITKLNAVVGRGGLLKPMVGGTYHVNGKMIEDLRGAV
                     MGQHASNLGGIIAHEIASQLGIQAFIVDPVVVDEMDEIARISGMPDLERISIWHALNQ
                     KAVGRRAAADLNRKYEDLNLIIAHLGGGISVGAHRKGRVVDVNNALDGEGPFSPERAG
                     GLPVSGLIKLCYSGKYTLDEIKKKITGKGGLVAYLGTNDGREVVRMIGEGSKKAELVY
                     RAMAYQVAKEIGACAAVLEGKVDAVCLTGGLAYDKLLTGWIKERVEFIGEVKIYPGED
                     EMIALAEGGLRVLRGEEAAREYK"
     gene            complement(13959..14861)
                     /locus_tag="PKI50_08460"
     CDS             complement(13959..14861)
                     /locus_tag="PKI50_08460"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HNT03770.1"
                     /translation="MIRTFKETIESAKAKGPKTIAVAVAQDPEVLSAVNAAKKLGIAE
                     AILVGDREEIIKASEECGIDIGKYEVIDIKDKNEASRKAVEMVSGGRAHIVMKGIVDT
                     AIVLKAVLDENIGLRTGNVLSHVAVFEVPGYDRLFYVTDPAMILAPSLIQKKQIIENV
                     VQVANALGNNNPKVAVLAAVEKVNPKMQATLDAAELVKMNESGELKGCVVGGPFALDN
                     AISVEAARHKGVTHPVAGLADVLLVPFIEVGNVLYKSMVYYAGSKVAGILVGAKAPVV
                     MTSRADSDEAKLNSIAIAVLMAGK"
     gene            complement(14862..15944)
                     /gene="buk"
                     /locus_tag="PKI50_08465"
     CDS             complement(14862..15944)
                     /gene="buk"
                     /locus_tag="PKI50_08465"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNT03771.1"
                     /translation="MSREYILAINPGSTSTKAAVFKGEENILQKNLSHSAEEVSKYEK
                     ITDQYEYRQKMILDWLKESDISTGSLAAVVGRGGLLKPMPGGTYIVTDAIIDDLRSAV
                     QGEHASNLGAIIARGIADTEGIPAFIVDPVATDEMDDIARISGMPELERPALVHALNI
                     KAVGRRAAKDMGKEFTDVDFVIAHLGGGISVSPVRKGKIVDVNGANDAGPFSPERAGG
                     LPVGNIVKMCFSGKFTQAEMKKKAVGSAGLVGYLGTNDARDVVKMIEAGDRKAALIFG
                     AMAYQIAKEIGAMATVINGKLDAIILTGGLAYSDLLCREITKRVEFIGPVLIYPGEDE
                     MQALAEGALRVLKGAEKAKIYEEEVK"
     gene            complement(16233..17948)
                     /locus_tag="PKI50_08470"
     CDS             complement(16233..17948)
                     /locus_tag="PKI50_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNT03772.1"
                     /translation="MKKDIELILNSTHDAMIGVDTDGIITLFNKAAERLTGIEAKDAM
                     GKYVADVIKDTRLPYILETGESELNRQQDLGNIKIITNRMPVKDKKGRIIGAVAVFRD
                     ITEIIKLAEEITNLNEVYSLLEAIINATQDAISVVDQNGLGVMINPAYIKITGLSEKD
                     VIGKPATVDIAEGESIHMQVLKTRKPIKGALMKVGPARKEVLVNAAPIIVNGELRGSV
                     GVIHDMSEITKLSNELKNAKNIIRSLEAKYTFEDIIGSDPLLLSAIDKAKKAAEIPIT
                     VLLRGESGTGKEIFAHAIHNESERKFRKFIKVNCSAIPETLLESELFGYEEGAFTGAK
                     KGGKKGLFEEAEGGTIFLDEIGEIPHSTQTKLLRVLQEKEIVRVGGTKSISIDVRIIA
                     ATNVDLEVAIESGRFRADLYYRLNVLPITIPPLRLRKGDIYDLSIFFIKKFNQQYGRN
                     VRDITQQAVSRLKEYNWPGNVRELDNFIGRAIINMKASDTVIQENHLPKLEGFLDAGD
                     NPDARMKGRGSISMTLDSVVEKAEKEHIAKVMKECGNNKTKAAKNLNISLRSLYYKLE
                     RYGLE"
     gene            complement(18088..19719)
                     /locus_tag="PKI50_08475"
     CDS             complement(18088..19719)
                     /locus_tag="PKI50_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNT03773.1"
                     /translation="MHFEVNNLKQPVRDFPFDNDADMVRFLAAEKLRIREKDISSLRI
                     LRRSVDARDKKDILLVYSAEVEINESTSPSRYLRVKDVARVNERAEYEEENKLVYGSR
                     PINNRPIIIGSGPAGLFAGLVLAQHGYSPLILERGSDVDKRAEAVNTFFRQGVLDEES
                     NIQFGEGGAGTFSDGKLTTRIKDRRISRVLNTFVEEGAPKEILYQAKPHIGTDILKTV
                     IKNMRKRITKLGGEVRFLSKVTDILVEEGRAKAVIVNGKSAIESNIIIAALGHSARDT
                     YGMLLERGVRIIPKPFSIGVRIEHLQSMIDEAQYGSFAGHPRLGAADYLLTDRSSSTG
                     RAVYSFCMCPGGQVVAAASERGMLVTNGMSEYKRDRENANSALVVSVSPDDFKSSHPL
                     SGVEFQRIWEGKAYKLGGGNFHAPVQLAEDFLKGKASRAAGRVKPSYEPGVKPCDLHL
                     CLPEYVTLSMEEALRDFDRKIKGFACCDALMTGVETRTSAPVRIVRKDNMESESVENL
                     YPVGEGAGYAGGIVSAAVDGIKAAERIIQNFKPIK"
     gene            complement(19732..20958)
                     /locus_tag="PKI50_08480"
     CDS             complement(19732..20958)
                     /locus_tag="PKI50_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNT03774.1"
                     /translation="MNNKLINKDHTIRILSVMLALLLWFYVITEQNPEITKDVTVPIR
                     LINTVFLEKSNMVIVNDTDNFALTMRIKGKKDVLDKLNANIVDAYADLAGHNLKGENF
                     LKINVDGIPEGVNIITRSMDSLKIMLESKISVQRSVKLNIMGNPTHGMAIMMHSLTPS
                     DVVITGAESKIAMIHEVRVDVDIASANAEVRKVLPVRVLDKTGKDIQDITVEPGNVEV
                     SIPIENTKWVSLSMDILGQPAAGFVVSGASVQPEEVLVTGKQQILEGITSIKTEKIDI
                     TEVKAAVSKEVRLVIPEGLEIVNADEIVKVSVNIEKIATSEIIVKDLEYVNLPEDLEL
                     VSIQGDIKITLRGAESRIADAPRTVKYYVDLKDAVEGSNVLNVLWEAPQGIEVLEVSP
                     NQAAVVLISKAKLQEP"
     gene            complement(20951..21769)
                     /gene="cdaA"
                     /locus_tag="PKI50_08485"
     CDS             complement(20951..21769)
                     /gene="cdaA"
                     /locus_tag="PKI50_08485"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNT03775.1"
                     /translation="MDEFKELLPYLKMWNTYVDIGIIAIIIYKAMNLIRETRAEQLIK
                     GIAMLLIATKLSEVLGLHTVYWLLKNAMTVGVIAILIIFQPELRRALEHIGRGRFFIK
                     GELLEHEIDSMLDEVCVAVAQLSKSKTGAIIVIEQETGLNEYIETGTKLDSVVNNEIL
                     INLFVPNTPLHDGAVIIRRNRIAAAGCFLPLTQSQNLSKELGTRHRAAIGITEISDSM
                     VIIVSEETGVISFAKMGRLSRYLDTKSLREIIKNSIKARENKQPIWMKWGVKNE"
BASE COUNT         5210 a         5731 c         4019 g         6794 t
ORIGIN      
        1 aaatccttaa taaagtttac tccggatata acttctccga atactattcc cttgtactct
       61 ttaaccttcc tgccttctac attgggtgtt gtagttataa tcatcctaat ccctccgaac
      121 atttataacg tattactatg tactactatt tgtattccca ttttataaat tataagtaca
      181 gatattaaag tttatttaat tcaacttcgg caaattcctt tgcggcaagc agctcctctg
      241 cactgggatg cctgcggttc atgatagccc agcacttgcc cctgcagccg aagctttcag
      301 ccgatacatt tatcccctgt tcctccaaaa gctccttcat aattttgatt gccttttcct
      361 gcattccata ggttccgaaa gcagcagctt ttttaaccat actgccgttc aatgtcttga
      421 taaagtccac agttgaatta tccggtctcc ctccgtaaac tccatctccg ataaacagca
      481 tatcaacagg ttcagcaata ctgccttcac ctatcctttc agccttaacc cccaaagcct
      541 cagccatagc ttccgctact ttccccgtat tcccgtttat agtatgatac attaccttga
      601 tattcatttt acatccctcc aaatcatgct aatcaaacat cccaatacaa aatacgttaa
      661 taactgtaaa aagtctgata tattgcttat atatcagacc tgaatccttg gactggtacc
      721 aggtatagag cttatactat atggaatatc ctcatttctt gctccttcta tttcagcttg
      781 cctctgcaat ccaccgggat ttcctctatt acctccccat tggaaacaat ataggctgtt
      841 tcatgcaggt tgactacagg gcatatatga ttaggaataa tctccacctt gtccccgact
      901 ctgattgaat catgaaactc cttgttatat ataattgcat gctcatcata tacggcatgt
      961 atgcttactc ccggccatcc tttaatcagg cccaggcctt ttgttgcagt aaagccttta
     1021 tttctggttt gcgcagtaag ccccttggct ccgacatcag taatcacccg ctccggagta
     1081 ggcaaactga ttacggtagt cagaacggtt gcagcattgc gttcaaggct tccgtatgca
     1141 tttccctggg acgcatccat gaaaatatat gtgccgggcc tgatttcagt tactccgtcc
     1201 agtatgggaa aatcatgcat caaggaaggt gtggaaccga tactgactgt ttccaaatga
     1261 aagcctgctt ccgctgccag ttgggcaaac cgtagcgtcc tttcctgtgc tttcaaatgt
     1321 atttccttac atgtctccgc attctccgct gcataggaat gaccgtcatg ggagaaaatc
     1381 ccttttaaat atatgttact gcacttcttc aaatggttaa caagctcaac aaaggcagct
     1441 tcttcaataa cgccggaacg ctgttccccc acttcaatct cgattagtac aactgcctgc
     1501 tttttactgc cttcaaaagc cttctctatc atatctgcct gcgggatact gtcaagcccg
     1561 aaggatatat caattgtttc agccaacttg cggattcggc gcagtttttc ttcaccgact
     1621 atctcatttg caatgaatat atctctcagc cctttggctg ccatgacttc cgcctcgcca
     1681 accttggcca cggcaatacc ttttgctccg agttcttcct gcaaaagagc cagcctggac
     1741 attttatggg tttttgtgtg gggcctcagt gccacattgc agcgattcgc ataatcctgc
     1801 atccttctgc aattacttat cattatatcc ttatcaatta ataatgccgg tgtatccaat
     1861 tcccgataat tcattacttt ccctcctttt aagccgcttc agtgatcaga tttgaggcct
     1921 gctccgcaca tgggtatcag gcagtcagat atccgcccgt ttaattcaca atacttcaga
     1981 taggctgcat aagtggctgc tgttgtatgc tcacagtaaa taccctttgc agcaagggct
     2041 gctctggctt cctgaatccg attttccgga gccgtgatta tatcaatttt atacttgtat
     2101 gtatattcca ggatttcctt accccgcatc ggcacgccga cggcaattcc ctccgccatt
     2161 gtgggctgtg ggattacctt taccgggtgc ttttctccgg ttataactgc ctttgccaag
     2221 ggatcgcagt gctcactttg cactgctatt atcttcggca ttttgtcaat gacggcactc
     2281 tccaggagtt cttccaatgc ctttacaatg ccgatgaaca gcgtaccgtt gccgaggggt
     2341 acaaaaagat tctcggggat tctttccatc tgctcataga cttcataaat atatgttttt
     2401 gtaccttcat agaaaaaagg attatacaca tggtttgcgt aatactttcc ttctgtctcc
     2461 accttcgctc tgcatacatc cgcacaattg tcccttgtgc cgggaacgac atttactttt
     2521 gcattgtggg atttaatcat attaatcttc cggggcgatg ttccttcggg tacaaaaatc
     2581 tcgcagccga tacctgcttt tgcacaatat gccgccacac tgtttccggc attgccgctg
     2641 ctgtcctgca caacggaatc caccccgatt gccttgcagt gggcaatcag taccgcagca
     2701 cccctatcct tgaaagacaa agtcggcatg aaataatcca tcttcagaag tacatcttca
     2761 ttaaaccgaa taacaggtgt tatgccttcg cccaggctga tttcccgcca gtgcgcccct
     2821 tccatcggca taaaagcacg ataccggaaa atgctccagg tatcatgatc caccaaagac
     2881 atatcaaact tcggaggttt gtaatccaat ttccacaggc ctccgcagtc acacttcgct
     2941 ttttttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata aacagaggag
     3061 aaattttatg gaaaacaggt atgtgaattt taatgaaaag cggatgtaca tattcagcga
     3121 gcttgtaaag agatactgga acggaggcct ggagaccgcc catgatctca ccgcactgac
     3181 cgattatatt aagcggaaat acggcttcag cgattatgaa ttgccattta taaaggatca
     3241 tataagagtg gcgatgggat tggatcctaa aggagacggc acattcactg atgaactgga
     3301 cataattaaa agtttcaggg aaattgaccg gcctgtggtt gccaggattg agagcccctg
     3361 tgaacattgc ggaaagttag attgtgaatg cggcaaatgc aaacatacag aagcaggcaa
     3421 gtacgaaact catatgtata gcagaaaaag tgaaccgata atatccaatg ataagtgtct
     3481 aaactgcgga tacagtgctc ctgattgtga tttcggggca ttggctgaca agattgaatt
     3541 cataccggtt attgatttgc tgaaagacga aaaaacacct gtcttcgctt gtgcagcacc
     3601 ggcgatagtc gggcagttcg gagaagacgt cactatgggt atgctgcggg ctgccttgaa
     3661 gatgattggc ttcaccgata tgattgaagt ggctatgttt gccgatatac ttaccataaa
     3721 ggaagccttt gagtttaacc aacttgtaaa aagtgaagag gattttttcc tgacaagctg
     3781 ctgctgcccg gtatggttca acatgattaa aactaattat cccgacatct ataaacacct
     3841 gtcaccctcc gtgtcaccaa tgatcgcttc gggcagaatt ctgaaaaaac tatataatga
     3901 tgccaaggtt gtatttattg caccctgtat tgccaaaaag gccgagatta aagagccgga
     3961 cttaaaagga gccatagatt atgtcttgaa tttcagggaa ctgaaagaaa tattcaatgc
     4021 gctgaatatt aatccgggag agcttccggc agatgaaaaa gatcaggcat ccttcggagg
     4081 cagagtatat gccagaaccg gaggcgtaag cttttccgtc aaaacggtgg tcaataggct
     4141 tgaaccaaca agagtcatta agcttaaagc aaaaaaggtt gacggagtga aagaatgcag
     4201 gagagtactg gatgatctgc gtgacggcag aaaaatcaat gccaacttta ttgagggtat
     4261 gggctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgc agcctttttc
     4381 caccgccatg tagtaagcta agagctgcag gggcattact gccaccaccg gagccagaat
     4441 gtctgatact ttggggatgt agagggtgta gtcagccaca ttctcaacct ctgtgctgcc
     4501 ttcctttgct aaggcgaata caaaggcccc tcgcgccttt acctctttta cgttgctcac
     4561 catcttctcg tacagctcat cctgggtgag gactgccaaa actatcgtgc ccttttctat
     4621 aagggctatg gtgccgtgct tcagctctcc ggctgcataa gcctcagagt gtatatagga
     4681 tatctcctta agcttcaggg agccttcaag ggctacggca taatctattc ccctgcccag
     4741 gtagaatatg tccctggcgt tggaattatg ggtcgcgaat ttctggatgg ctgatttata
     4801 tttaagggtt tcctcaacaa gtccgggaag ccttctcatt tcatccttta ttgattcaat
     4861 ttcctcatcc ttcagggtac ccttcagctt agccatgtac agtgcaatta tatagattgc
     4921 tataagctgg gttgtgtatg cttttgtaga tgcaaccgca atctcgggcc ctgcccatgt
     4981 atataacacg tcatgggatt cccgggctat tgagcttcct accacattga ctatgctgag
     5041 tatcctggag ccttttctcc tggcctcctt caaagctgcc agagtgtcca gggtttctcc
     5101 cgattggctc actattatcg tcagggtttt ttcatccact atgggatccc tgtatctgaa
     5161 ttccgaggct atttccactt ccacaggaat tcttgccagc ttttctatta cgtatttgcc
     5221 caccatgcat gcatgataag ctgttccgca ggcaaccatg aaaattttat taaaattctg
     5281 cagttcctcc ttcgtaagct ttatgtcatc cagctttatt ccggcggaat ccggcagcac
     5341 tcttgaggtc atggtatcct tcacggcttt gggctgctca tgtatctcct ttatcataaa
     5401 gtgctcaaag ccgccttttt cagctgcggc aacatcccaa ttcacttcga aaacttcctt
     5461 gccgacctcc ttgccgcttt catcaaagaa gcttattctg tccttctcaa tcactgccag
     5521 ttcattctca ttcagcaggt atatccttct tgtatggttc agtattgcag gtatgtctga
     5581 ggcaatgaaa ttttcaccct tccctattcc cactatcagg gggttttcct ttcttacgca
     5641 tattattttg tcaggctctc ttctgcttat cacggcaaaa gcataggacc ccctaatttt
     5701 ttcaagaacc ttcattactg cctcaagcag gtcagtctta tagaagtaat ccacatagtg
     5761 cgccagcacc tctgtgtcag tttctgacct gaatgtatac cccttctcta taagcatgct
     5821 tttaatgccc atgtaattct caattatacc gttgtgaact actgcaatat caccggaaca
     5881 attggtatgg ggatgcgaat tcacatcgga gggttcgccg tgggttgccc atcttgtatg
     5941 ccctatcccc aggcatccgt ccaaggtctc cttcttcaga tgctcttcaa gtacggagag
     6001 cctgccttta tatttcctta tctctatgtt ttcctttgta tataccgcca ctccggcaga
     6061 atcatagccc ctgtattcaa gcttgcgcag cccttccatc aagatgggca ctgcgttttt
     6121 atctcctata taaccgacga tgccgcacat atataaaatc ccctctcaaa tattgtaagt
     6181 ggataagtat ggtgcctggc actcaattcg taagtggata agtatggtgc ctggcactca
     6241 attcctcaat tcaacttttc ctctatgagc ttagccaggg cctcagcatc ttcctttatt
     6301 tccgtctcgt tagctccctc cagcataacc ctaatcaagg gctcagtgcc ggaaggcctt
     6361 ataagcactc ttcccctgcc cttgagcttt ttctccagtc ctgttatcat tcctgctata
     6421 gcttcatcct tcatatactt atccttgttc tcattcttca ccttcgcgtt taccagcacc
     6481 tggggcagtt ctttcataaa ggaagcaagg tcggacagct tcttgccggt ttccttcaca
     6541 actgccagca gctgcagggc tgtcaaaagc ccgtctccgg tagtattatg atccaggaat
     6601 attatatgtc cggactgctc gccgccaatg gagtaaccca ttgaaaccat ttcctcaagt
     6661 acatacctgt ctcctacctt agtctttaca agattgcact tctcccgctt caaagctata
     6721 tcaaggccca tgttgctcat gacagtagcg actaccgtgt ctttcttcag catgcccttt
     6781 ccttttaaat gcctggcaaa tatggcaagc atgtgatctc cgttaacaat atttcctctg
     6841 ttgtctactg caataagcct gtcagcatcc ccgtcaaagg ccagccctat atgggctccg
     6901 gattccacaa ccagcttctg caggccttca gggtgtgttg agccgcattt tacgtttatg
     6961 ttctttccgt tgggcttgtt gtttatgaca gctatctcgg caccaaggtc cgcaagtacc
     7021 gaaggcgccg ctatatatga tgcgccgttt gcgcaatcaa ccgctatctt cagccctctc
     7081 aggttgacat ctatggtctt tttgagaaaa tcaacatatc cgtgtaccgc gttatctacc
     7141 tcaaccttct tgcctatatc cgcccctgtg ggatatgcta tgccgttttc cggattcagc
     7201 atcaggcttt ctatcctgtc ctccaattga tccgacagct tatatccttc accgttgaaa
     7261 aactttattc cgttatattc aaagggattg tgcgaagccg aaatcacgac tcccgcatcc
     7321 aggcccatat gcctcgtaag gtatgccact gcaggcgtag gcaaaactcc cagggacacc
     7381 gcttctgctc ccatcgagca tatacctgca atgagggcgg cttcaagcat gtcaccggag
     7441 attctggtat ccttccctac agcaattctg ggttttttgt gggtttcttc cgttaataca
     7501 tatgcaccaa ttcttccgag attgaacgct aaagcagggc tcaactcttt gttggcaatc
     7561 cctcttactc cgtctgttcc aaacatgcgt cccattatat gacccctttc tcaacagtga
     7621 aggtcatttt cactgatgat atgatttttt tggatatagt aatattatca taactcatat
     7681 gttttgttca aatcattaca tgttatcggg tgtttctatc cccgcaaggc tcaggcctgt
     7741 cttgataact attccgaccg ctttggtaag ctgcagtctt gcattcttga cattttcgtc
     7801 agcattcagt atgggatggg tgttgtaaaa tttgttatag gccttggcta tctccgttac
     7861 atggcggaca actacaaagg gctcatacct ttccactgct tcctttaccg aatccttgaa
     7921 ggctcccaga agcttgacca actggaattc atcctctgtg gaaagaaaag tcaggtccgc
     7981 cgtatccggt acgtatcccg ccttctgcaa tatgctcctg cctcttacat aggtatactg
     8041 aacatagggg ccggaatctc cgtcaaagtt caatgcatct tcccaggtaa acacaacatc
     8101 cttttccctg ttattcttaa ggaaggtata aagaagcgct cccatgccga ccttccttgc
     8161 aacctcctcc ttgttctcca ggttcggatt cttatgctct attacttcca tagtcttatg
     8221 tacagcttca ttcattacat cctttgcaaa aacaacgtcg cccttccggg tcgaaagctt
     8281 tttgtctgca aactttacaa ggccgtggcc tatgtgaacg cagtcctcgg accactcaaa
     8341 gcccataagc cccagggtgc tgaaaatctg attgaaatgc agggactgtg ttcctcccac
     8401 cacatagatg ttcttataga aatcgtaggt cctcttcctg tatatggctg ctgccagatc
     8461 cctggtggca taaattgtgg caccgtcaga cttaagtatc attatcggag gtatgccgaa
     8521 ctgctccaga tcaactattt ttgctccgtt gctgtcctta agcagtttct tttcacgaag
     8581 tatttggaca acctcatcca tcttgtcact gtaaaagctt tcgcctgcat atgaatcgaa
     8641 ctccacattc agcatctcat aaatctcgct gaattccctg aggctcagat ccttgaacct
     8701 ttgccaaagt atggttactt cggaatctcc ttcttcaagc tttttgaagt atgctctggc
     8761 ttcatcttca agggccgggt ccttttcagc ctcttcatgg aatttgacat atatccggaa
     8821 aagctcgctt atgggttcct tctcaagggc ttcttcattt atccatctct tatgtgcgga
     8881 tataagcttt ccaaactggg ttccccagtc tccaagatgg ttgattctta tcgtattgta
     8941 gcctgagtgc ctgaaaacct tttcaagggc gctgccaagt attgtattga acaggtggcc
     9001 tacatggaag ggctttgcta catttacgga agagaattca actattacat tcttatcctt
     9061 gccttcctcc gagcttccga atttctctcc cttctcaaga atctcctgta tcataatcct
     9121 tatgtattcc ttcttattaa tgaaaaaatt cacatatccc cctactgctt ccgccttttc
     9181 aactatgctg tcagcctgca ccttccccgc aatctcggca gcaatcatat tcggtgattt
     9241 tctcatcact cttgccagct gaaaacaagg aaaagcataa tcccccatat tagtctgggg
     9301 cggaatctct ataagctcca tgaccttatc aaattccata tcaactaacg tatttatttt
     9361 ttttgcgatt tccactttga aattcatatt tatccctcca ataagtttta ttatatataa
     9421 aatgcagaaa aaaatccatc ccgaaaggag gatggaagat ggtggttttt tattttcggg
     9481 atggatttaa ttaaattatt ttatgcaagc tgcccctctt aaaagtgctt ctttattcag
     9541 cggtataacg ctggctttct tacctgcgaa aatatgtgtg ataatattca ttattgaatc
     9601 gaaggatgct gcacctgatg actggagata tgctcccagc attactatgt ttgcaacctt
     9661 gtctgttccc aactcgtttg ctatttcgtt tgccggcacg tagtaaacct ttatatcttc
     9721 tcttgtggct ttcttgtcta ttaaagagct gtttatcaaa agtgctccgc ctggtacaac
     9781 agtattttca tacttgtcca gtgacggtct gttcattact atagctgctg tggcttccgt
     9841 aactatcgga gcaccgatag gctcatcaga aactattacg ctgcaatttg ccgtgcctcc
     9901 acgcatttcg gggccgtatg agggtagcca tgacacattc tttccttctt ccatggctgc
     9961 ctcaacaagt attttaccca tagccatgac gccttgtccg ccaaagcctg ccattataat
    10021 ttcatgtgcc attatttcac ctcctcaata tttttgaagt ttccaagggg ataatatggt
    10081 atcatattct cctggagcca cttcaatgct tctataggag ttttacccca gtttgtcggg
    10141 caggtagaca gtacttcaac aagagtaaaa cctttgcctt ccatctgaag ctggaaagct
    10201 ttctttatag ctttctttgc ctgaataata tgctttacgt catgtaccga tactctttct
    10261 acaaatgcag ccttaggaat agttgcaagc atttcgctta ctcttaaagg catacctgca
    10321 tggttctcgt cccttccgta aggggctgtg gttgccttct ggcctacaag agttgtagga
    10381 gccatctggc cgcctgtcat tccatatatg gcattgttta caaatattgt ggttatcttt
    10441 tcgcctctca ttgccgcatg gacaacctcg gcagcaccta ttgatgccag atccccgtct
    10501 ccctggtaag taaacactgc agcatttggg tttactctct ttatgcctgt tgcagcagcc
    10561 ggagctcttc catgagctgc ttcctgcata tcgcagttaa aataatcata tgcaaatacg
    10621 gcacatccta caggagcaac accaatggca tcatctataa gattgagctc atcaagtgat
    10681 tcagccacca acctgtgtat tatcccatga gtgcagccgg gacaataatg gaagggcttt
    10741 tctgcaagtc ctctggtctt ctcaaataca actgccatta ctttgcaccc cctaatatct
    10801 tcttagcttc ttcagcaata gccttaggag ttgggaacat accgcccgtt cttccataga
    10861 agtaaacagg tttctttccg tttacagaca gcttcacatc ttcaaccatc tgtcccatac
    10921 tcatttctac tgacatgaaa gcttttacgc cggcctgttc tgctgctttc tcaaaggctt
    10981 tggccgggaa tggccaaaga gttatcggtc ttataagtcc tgcttttatt ccttctttag
    11041 caagggagtt tattgcattc ttaactattc ttgctgttgt tccataagca gcaaatacta
    11101 tttctgcacc ttcaaggtta tactcttcaa ctttgatttc attcttttcg atttccgcat
    11161 atttttcaaa caaatggaag ttgtgtcttt cacaatctgc cggatctata aacaaggaat
    11221 ttatgacatt agtcttcctc tttcccttgg tacccactgt agcccaagtc ttttcaggca
    11281 gttttctctt ttttacttca gtaaattcca ccggctccat catctgtcct atcataccgt
    11341 ccccaagaat caatactggg ttcctatact ggtctgctat gtcaaaggct tcctgagtca
    11401 agtcaacggc ctcttgtact gaagcaggtg caagcactac caacctgtaa tcgccgtgtc
    11461 cgccgccctt gcaagactgg aaatagtctg attgtgcagg ctgtattccg cccagtcctg
    11521 ggccccctct taccatattt actattacac aaggcagttc agctcctgct atatatgtta
    11581 taccctcctg tttcaagctt attcccgggc tggatgagga tgtcataacc cttgcacctg
    11641 caccagctgc tccataaacc atattaatag cagctacctc agattctgcc tgaaggaaac
    11701 atccgtcaat ctgaggcatt ttcatcgcca tgtactcagg tatctcattc tgcggtgtaa
    11761 tcggatagcc gaagaaaaat ctgcagccgg ctttaatcgc cgcttcagct acggcctcgt
    11821 tgcccttcca taattcctta gccattaggc ttctacctcc ctttttatct ttctactttt
    11881 attacaacgt caggacacat tcttgcacaa gaagcacatc ctgtacattt gtccatttcc
    11941 ttaactgtcg ccggatgata acccttgata ttaatcctat ccttatccat aacaacaatt
    12001 ttgacaggac atactgtggt gcatagctca caacctttac atctgttttc gtcgaaagtt
    12061 accttagcca ttacttaacc tccttactaa tttgttttgt tagttacaat gctgatataa
    12121 ttagtcctcc caaggctttt tcatatatat atttattggc aatacatcac ctcggagtcc
    12181 tttcgggagc acgtcaacaa acttcttttc aacaaccaca tacttgattg gcagccccag
    12241 aaccttggat acatcttcac aaagcttctg ccccctcatt atatcctcta cagtggtatt
    12301 gccgcagaga tgagtattgt taaccaatgc agtaaccttt tgcatagagc cgtgttcaat
    12361 ggatcttaag tagcttattg ccccatccac tgtactggtc tgatgtctat tggcattcag
    12421 cacaaaaaac atatcataag gctccctgtc aaagtactcg tggtatcttc ccagagccct
    12481 ggctccgacc ttgtctccgc cgacatccaa tacaacctca taagaggtat cctgcaaaac
    12541 ggagtatatt tccggtgaaa gggcgggcag gtctccgaat atgcctttta ctgaagaaga
    12601 tataaccctt atgccctttt ctaccagcag cggttctctt tcccttgacc ggaagtatgg
    12661 atttactatg tccaagtcaa ctattgctgt cttctttccc gcttccgcaa gagcaattgc
    12721 ataattcaca gcaaactcgg tttttccgct tccgtagtgg cctgtgatta tccttactct
    12781 tctatcgtta tacatttaat catccctatc aggctgtgca gcctatttat actcccttgc
    12841 agcttcttcc ccacgaagca ccctgagccc gccctcggca agagctatca tttcatcctc
    12901 gccgggatat atctttacct ctccaatgaa ctctaccctt tcctttatcc agccggtaag
    12961 aagcttatca taggcaaggc cccctgtcag gcagacggca tccacctttc cttcaagcac
    13021 tgcggcgcag gcacctatct cctttgccac ctgatatgcc attgccctgt acacaagctc
    13081 tgctttcttg ctgccctccc ctatcattct tacaacttct ctgccgtcat tggttccaag
    13141 gtatgcaaca agtccgcctt ttcctgtgat tttcttcttt atttcatcca atgtatattt
    13201 acctgagtaa cagagtttta tgagcccgct gaccggtaat cctccggctc tttccggaga
    13261 gaatgggccc tcaccgtcaa gagcattgtt tacatccacc actctgcctt ttctgtgtgc
    13321 gccgactgat attccgcctc ccaaatgggc aattatcaga ttcaaatcct catactttct
    13381 gttaagatcc gcagctgccc ttctgcctac ggctttctgg ttaagtgcat gccatatgct
    13441 tattctttct aaatcaggca ttcctgagat tctcgcaatt tcatccattt cgtccaccac
    13501 aaccgggtct acgataaatg cctgtatccc aagctgggaa gctatttcat gggcaattat
    13561 ccctcctaag tttgaagcat gttgtcccat gactgcaccc ctaagatctt caatcatttt
    13621 tccatttaca tggtaggttc cgcctaccat gggctttaag agtccgcccc ttcctacaac
    13681 tgcatttagc ttggtaatat ttatcccatt ggcattcaat gtatcaagta ttacattctt
    13741 tctgaattca tattgctcaa taattgctcc aaagggggca agctcctcgt tggaatgtcg
    13801 gagagtctcc tccagaacag gcttctcatt atcaaaaata gcaatcttcg ttgaggtgga
    13861 acccggatta atgatcagca acctgtattc ctgcatttta aaacaacctc ctaaatttgc
    13921 ggaacccctt caatggatcc gctgctcgta tcttttatct actttcctgc cataagtact
    13981 gctatggcaa tggaattcag cttggcttcg tccgaatcgg cacgggaagt cattaccaca
    14041 ggagcttttg cccctaccaa tattccggca acctttgatc ccgcataata taccattgat
    14101 ttgtacaaaa catttcccac ttctataaag ggaaccagca agacatccgc cagtcctgca
    14161 acgggatggg ttaccccttt atgcctggct gcctctacgg atattgcatt atcgagtgca
    14221 aaggggcctc cgacaacgca gcctttcagt tctcccgatt cattcatttt taccagctct
    14281 gccgcatcaa gggtagcctg cattttggga tttaccttct ccacagcggc cagcacagca
    14341 acctttggat tgttattacc cagggcgttg gccacctgta cgacattttc aattatctgc
    14401 ttcttctgaa tcaaactggg agccaatatc attgccggat ctgtaacata gaaaagcctg
    14461 tcatatcccg gcacttcaaa cactgcaaca tgagaaagaa cattgcccgt tctgaggcct
    14521 atgttttcat ccaaaaccgc tttcagcaca atagccgtat caactatacc cttcatgaca
    14581 atgtgtgccc tgccgccgga aaccatttca accgctttcc tgctggcctc attcttatcc
    14641 tttatgtcaa taacttcata tttgccgata tcaatgccgc attcctcaga ggctttaatg
    14701 atttcttccc tgtcgcctac aagtatggct tccgcaatac caagcttttt tgctgcattt
    14761 accgcactta atacctccgg atcctgagca actgcaacag caatggtttt gggccctttt
    14821 gcctttgcag actcaatagt ttccttaaaa gtcctaatca tttatttcac ctcttcctca
    14881 tatatctttg ctttctccgc acccttgagt actcgtaaag caccttcagc caatgcctgc
    14941 atttcatcct ctcccggata tatcaggaca ggcccgataa attccactct tttggttatt
    15001 tccctgcaca acaaatccga atatgcaagg ccgccggtaa gtattattgc atcgagcttc
    15061 ccgttaatta ccgtggccat agcacctatc tccttggcaa tctgatatgc catggcgcca
    15121 aaaatgaggg ctgccttccg gtctccggcc tcaatcattt tgactacatc ccttgcatca
    15181 ttagtcccca aatatccgac gagtcccgcg ctgcccacag ccttcttttt catctccgcc
    15241 tgtgtgaatt ttcctgaaaa acacattttt actatattgc ctacaggaag ccctcctgcc
    15301 ctttccgggg agaaggggcc cgcatcgtta gcaccgttta cgtcaactat cttccctttc
    15361 ctgacgggag aaacggatat ccctccgcca agatgtgcaa taacaaaatc aacgtcggta
    15421 aattcctttc ccatatcctt tgcagctctt ctgccgacgg cttttatatt caaagcatgt
    15481 accaaagccg gcctctcgag ttcaggcatt cccgaaatac gcgcaatatc gtccatttca
    15541 tcggtagcta ccggatcgac aataaaagcg ggaatacctt ccgtatccgc tatacccctg
    15601 gcaattatcg ccccaaggtt tgatgcatgt tcaccctgaa cagcacttct caaatcatca
    15661 ataattgcat cggtaacgat ataagtgccg ccgggcatcg gtttcagcaa accgcctctt
    15721 ccaaccaccg ctgccaggct gcccgtgctg atgtccgatt ctttcagcca atcaagtatc
    15781 attttttgcc tgtactcata ctggtcggta attttctcat acttggacac ttcttccgca
    15841 gagtggctga gatttttctg gagtatattc tcttctcctt tgaaaactgc tgcctttgtt
    15901 gaagtggatc cgggatttat tgccaatatg tattctcttg acacagattc accgctcctt
    15961 acatatatta ttgcttatta ttcttttcag attatattta tgcaaatagt atgccacaaa
    16021 agcagtcgat aaaaataaaa acctgtcgtt tagtttatgt caaccaagcg acaggttaaa
    16081 aaatgttcat aatgcttata cttttttttg aaaaaaattt cagacattga ggctttcttt
    16141 ttgtttgtta cagaaaaaac ccggcatttg aaaaataatg catgcatatt cttgcaaatt
    16201 aatagtccgt tttgcaaata tttgcatgca aattactcga gtccgtacct ttcaagctta
    16261 taatacaggc ttcttaaaga tatgttcaga ttctttgccg cttttgtctt gttattgccg
    16321 cattctttca taacctttgc tatatgctct ttctcggctt tctccactac actgtccaaa
    16381 gtcattgata tgctgccccg gcctttcata cgggcgtcgg gattatcccc ggcatccaaa
    16441 aatccttcga gcttgggcaa gtggttttcc tgtataaccg tatcacttgc cttcatattg
    16501 ataattgcac ggccgataaa gttatccagt tctcttacgt ttccgggcca gttgtactcc
    16561 ttcagcctgc tgacggcctg ctgcgttatg tcgcgaacgt tgcgcccgta ttgttgatta
    16621 aacttcttta tgaagaaaat tgataaatcg taaatatcgc ctttgcgaag ccttaaaggc
    16681 ggaatcgtta tgggcagcac attcagcctg taataaagat ccgctctgaa cctgccgctt
    16741 tcaatggcca cctccagatc cacatttgtt gctgcgatta tccttacgtc aatacttatc
    16801 gactttgtgc ctcccaccct tacaatctcc ttttcctgca gcactctaag gagcttggtc
    16861 tgggtgctgt gaggtatctc ccctatctca tcaagaaata tagtgcctcc ttcggcttcc
    16921 tcaaaaaggc ccttctttcc gccttttttc gctccggtaa atgccccttc ttcatacccg
    16981 aacagctccg attccaacag ggtttcggga atagccgagc agtttacctt tatgaatttc
    17041 ctgaacttcc tttcactttc attatgtatg gcatgggcaa atatctcttt gccggtgccg
    17101 ctttcccccc tgagcaaaac ggttatcgga atctccgcag ccttctttgc tttgtctatg
    17161 gcagacagaa gcagcggatc gcttcctatt atatcctcaa aggtgtattt agcctccagg
    17221 cttcttatta tattcttggc gtttttcaat tcattggaaa gctttgttat ctccgacatg
    17281 tcatgaataa ccccgacact gcctctcagc tctccgttaa caattatagg ggcagcattc
    17341 accagtactt ccttacgtgc aggccccacc ttcattaaag caccctttat gggctttctg
    17401 gtcttgagta cctgcatatg aatgctttcg ccctccgcaa tatcaacagt agcaggcttt
    17461 ccaatgacat ccttttcaga cagtccggta attttgatat aggccggatt tatcattacc
    17521 ccaagcccat tctggtctac aaccgatatg gcgtcctgtg tggcattaat aattgcctcc
    17581 agcaagctgt acacttcatt caggttggtt atctcctccg caagcttgat aatctccgtg
    17641 atatccctaa ataccgcaac cgccccgatt atcctcccct ttttgtcctt caccggcatc
    17701 ctgttggtta ttatttttat attccccaga tcctgctggc ggttcagctc agactcacct
    17761 gtttccaata tatacggaag ccttgtatct tttattacgt cagccacata tttccccatt
    17821 gcatcctttg cttcgattcc cgtgagcctt tccgcagctt tgttaaaaag tgttattata
    17881 ccgtctgtgt caacaccaat catagcgtca tgagtagagt taagtataag ctctatatct
    17941 tttttcatac aacaaccttc cttacatcag gtccggggtt cggggttcga ggttcgggta
    18001 attggccggt tctttaaagc ctgccctaat cctcgcacct cgtgcctcgc acctcgcagc
    18061 ctgtttctag tggcctgtgt cttgaattta ttttatcggc ttaaagtttt gtattattct
    18121 ctccgctgcc tttattccgt ctactgccgc acttacaatt cctcccgcat agcctgcgcc
    18181 ctctcctaca gggtagaggt tttccacaga ttcgctctcc atgttatcct tccttacaat
    18241 tcttacaggt gcggaggttc ttgtctcaac tcctgtcatt aacgcatcac agcatgcaaa
    18301 accttttatc ttcctgtcaa aatccctgag agcttcctcc attgaaagag ttacatattc
    18361 aggcaggcac aaatgcaagt cacaaggctt aactcccggc tcatagctcg gcttgaccct
    18421 gcctgctgcc cgggaagctt ttcctttaag aaaatcttcc gccagctgta ccggtgcatg
    18481 gaagttcccg cctcccagct tatatgcctt tccttcccat attctttgaa attccactcc
    18541 cgataaagga tggctgcttt taaaatcatc gggagatacc gataccacca gtgcgctgtt
    18601 ggcattctcc ctgtccctct tgtactcgct cataccattg gtaacaagca tccctcgctc
    18661 cgacgcggca gccactacct gcccccccgg gcacatgcaa aagctgtata cagccctacc
    18721 ggttgaactg ctcctgtccg taagcaggta atccgcagct ccgagcctcg gatgccctgc
    18781 aaagctgccg tattgagcct cgtctatcat actttgcaga tgttctatcc tcacacctat
    18841 cgagaagggc ttgggtatta tccgcacacc tctttcaaga agcatgccgt atgtgtccct
    18901 tgcgctgtgt cccaaagccg ctattattat atttgattca attgcggatt tcccatttac
    18961 tattacagct ttagcccggc cttcttccac caaaatatcg gtaaccttgg aaagaaacct
    19021 tacttcccct cccagtttcg ttatcctttt tctcatattt tttatgaccg ttttcagtat
    19081 atcggttcct atatggggtt tggcctggta cagtatctct ttgggagcac cctcctccac
    19141 aaaggtattc agtaccctgc ttattcttct atccttgatt ctggtggtaa gctttccgtc
    19201 ggaaaaagtg cctgcccctc cttcgccgaa ctgtatattg gattcctcat ccaatacacc
    19261 ctgcctgaaa aatgtattta cagcttcagc ccttttatcc acatcgctgc ctctctcaag
    19321 tatcagcggg ctgtatccgt gttgtgccaa aactaaaccg gcaaacaatc ctgccggtcc
    19381 gctgcctatt attattggcc tgttgtttat cggcctgctg ccataaacaa gcttgttttc
    19441 ttcctcatat tccgctctct cattgaccct ggcaacatcc tttaccctga ggtaccggga
    19501 ggggcttgtg ctctcattta tttcaacctc cgcagaatac accagtaata tatccttctt
    19561 gtcccttgca tctacagatc ttctcaatat tctcagagaa gatatatcct tttccctgat
    19621 cctgagcttt tccgccgcaa ggaaccttac catatccgca tcattatcaa agggaaaatc
    19681 cctgacaggc tgtttcaagt tatttacctc aaaatgcaat taatcatctc cctagggttc
    19741 ctgcagctta gctttgctta tcagtacaac tgcggcttgg ttcggggata cctccagaac
    19801 ctctattcct tgaggcgcct cccataatac attcaggaca ttgctgccct caacggcatc
    19861 cttcaggtca acatagtatt tgacagttct cggcgcgtcg gcaatccggc tttccgcccc
    19921 tcttaaggtt atctttatat ctccttgtat acttactaac tccaaatctt ccggaagatt
    19981 aacatattca aggtctttaa caataatctc gctggttgca attttctcta tattcaccga
    20041 tacttttaca atttcatcgg cattaacgat ttccaaacct tccggtatta ccagccttac
    20101 ttcctttgat acagcggctt ttacttcagt aatatcaatt ttctcagttt ttatggaagt
    20161 tatgccttca agaatctgct gctttccggt tacaagcact tcctcaggct gtaccgaggc
    20221 accgcttaca acgaatcctg ctgccggctg tcccaatata tccatactca ggcttaccca
    20281 ctttgtattc tctataggga tgctgacttc cacattacct ggttctacgg taatatcctg
    20341 aatatccttt ccggttttat ccagcactct taccggaagt acttttctta cttcagcatt
    20401 ggcgctggca atatccacat ccaccctgac ctcatgaatc atggctattt tcgattccgc
    20461 tcccgtaatt acaacatcgc tgggagtcaa tgaatgcatc ataatcgcca taccgtgggt
    20521 cggattaccc atgatgttca gtttaacgct tctttgaacg ctgatctttg attcaagcat
    20581 aatcttaagg ctgtccatgg atctcgttat tatgtttacg ccttcaggta tgccgtcgac
    20641 atttatctta aggaagttct cccccttcag gttatggcct gccaaatccg cataggcatc
    20701 tacaatgttg gcattcagct tatctaaaac atccttcttt ccctttattc tcatggtcag
    20761 tgcaaaattg tcagtatcgt tgacaattac catattgctt ttctcaagga atacagtgtt
    20821 tatgagcctt attgggactg taacatcctt tgttatctca gggttctgct cggttatcac
    20881 atagaaccac aaaagcagtg ccagcatgac cgagagtatc cttattgtat gatccttatt
    20941 aatcagctta ttattcattc ttaacacccc atttcatcca tatgggctgt ttgttttccc
    21001 tggccttgat gctgtttttg attatttccc tgagggattt tgtatccaaa taccttgaaa
    21061 gcctgcccat cttggcaaag gatatcactc ccgtctcctc agatactatt attaccattg
    21121 aatctgaaat ttcagttata cctattgccg ccctatgcct tgttcccagc tccttgctca
    21181 gattctggct ctgggtaaga ggcagaaagc agcctgccgc agcaatcctg tttcttctga
    21241 ttatcacagc accgtcatga agaggggtat tggggacaaa caggtttatc aatatctcat
    21301 tgttcactac ggaatccagc ttggtgccgg tctcaatgta ttcgttcaaa ccggtttcct
    21361 gctctattac tatgatagca cccgtcttgc tttttgaaag ctgcgcaacg gcaacacata
    21421 cttcgtcaag catgctgtct atttcatgtt ccaataattc tccctttata aagaacctgc
    21481 ctcttccgat atgctccagt gcccgtctaa gttcgggctg gaatatgatt aaaattgcaa
    21541 taacccctac cgtcattgcg tttttcaaca gccagtagac agtatgaagg cccagaacct
    21601 cactgagctt ggttgcgata agaagcattg ctataccctt aatcagctgc tccgccctgg
    21661 tttctcttat taaattcatt gctttataaa taattatggc gataattcca atatctacat
    21721 atgtgttcca cattttgaga tacgggagca gttccttaaa ctcatccaat ctattcacct
    21781 cttatgcatg caatttcttt catacttcaa ttatataata aattccataa tgaaatatta
    21841 caagtttgtt tcaatttttt ccggatattc cttcatggta tggctgtgcg cggtttctaa
    21901 ctaaaagaac aggtgggtta tgtattggtc aattataacc aatgccccta ttat
//
LOCUS       DAOQUR010000037        21516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2861_length_21516_cov_9.782349, whole genome shotgun sequence.
ACCESSION   DAOQUR010000037 DAOQUR010000000
VERSION     DAOQUR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2861_length_21516_cov_9.782349"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(414..1283)
                     /locus_tag="PKI50_08490"
     CDS             complement(414..1283)
                     /locus_tag="PKI50_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNT03776.1"
                     /translation="MSENINLMKVMQTKFQKLSKGQKIIAQYIMKNYDKAAFMTAAKL
                     GEVVEVSESTVVRFAITVGYKGYPQLQKQLQEMVRTKLTTVQRIEMSSDYTNQASVLK
                     TILKSDIENIRGTMEEIDSSAFEQAVRAIIEAKSIYIIGLRSSNTLSEFLGFYLNFVR
                     DNVHVVTYTIGDIFEQLFRIGKDDLVIGITFPRYSTRTLKAFEYAKSRGANTIAITDS
                     ILSPLCANADYTLIAKSNMESFVDSIVAPMSLLNALIVAVGIKQKHDISDSFAKLEDI
                     WNKYNIFSADKDE"
     gene            complement(1299..1835)
                     /locus_tag="PKI50_08495"
     CDS             complement(1299..1835)
                     /locus_tag="PKI50_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT03777.1"
                     /translation="MERYEIRLSGSGGQGLLLGGIILAEGAINDGKNSVQTQSYGPEA
                     RGGASKSEVIISSEEIDFPKVRNCDILLSLTQKAYDEYNEGLKEDGILIVDSSVNAVK
                     SGNIKIYSIPIIDSAVKDLGKPMVTNIVALGVLVEITKVISKESLEKAVLERVPKGTE
                     ELNRKALAKGYEIANKLM"
     gene            complement(1835..2659)
                     /locus_tag="PKI50_08500"
     CDS             complement(1835..2659)
                     /locus_tag="PKI50_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNT03778.1"
                     /translation="MPSVLVENYFRTEKLPQIWCPGCGHGIITRSIASAIEKSGIDKN
                     KICIVSGIGCSSRAPGYLDFNTLHTTHGRALAFATGIKMAKPEIKVIVIMGDGDATAI
                     GGNHLIHACRRNIGITAIVYNNSIYGMTGGQYSPTTPKYDKGTTAPYGNIDKAFDICK
                     LAASAGATYVGRATAYHASLVTSLVQNAINNEGFSLIEAISMCPTYYGRKNKKGSAVS
                     MMEWQKNSAINISAAAKMSPEALEGKFLIGELKNSPEPEYTNEYQKIIDRLKKESQ"
     gene            complement(2661..3794)
                     /locus_tag="PKI50_08505"
     CDS             complement(2661..3794)
                     /locus_tag="PKI50_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNT03779.1"
                     /translation="MKSKVRLMQGNEACVEGAIAAGMRFYAGYPITPSTEIAELSAER
                     LPLVGGRFMQMEDEIGGMAAIIGASLTGAKSMTATSGPGFSLKQENIGYASIAEVPCV
                     IVNVQRGGPSTGMPTSPAQGEIMQARWGTHGDHPVIALTPSSVRETFDLTIKAFNFAE
                     KYRTPVILLMDEVIGHMREKVQLPDPSEIVIIDRKRPDCSSEEYLPYEADNDLVPPMA
                     DFGTGYRYHVTGLVHDETGFPSNSTAVADKLIRRLSNKIEIHRDEIESWEEENTEDAE
                     VLILSYGCVARSSKSAMNILRGRGIKVGTFRPITIWPFPEKRLRELAAKVKTIVVPEM
                     NLGQLVLEVERICGCNAKIEKLNKVSCEIIYPEEIVSKIEEVL"
     gene            complement(3778..4011)
                     /locus_tag="PKI50_08510"
     CDS             complement(3778..4011)
                     /locus_tag="PKI50_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT03780.1"
                     /translation="MEDIKAGEIQNEKVVINPAWCKGCGICVAFCPVKALELKNDKVV
                     MVDVDKCTQCGMCELRCPDYAIYLRRKKNEKQG"
     gene            complement(4160..4948)
                     /gene="surE"
                     /locus_tag="PKI50_08515"
     CDS             complement(4160..4948)
                     /gene="surE"
                     /locus_tag="PKI50_08515"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNT03781.1"
                     /translation="MLNILISNDDGIYSKGLYELAKRMAALGKVTVVAPDKEQSAIGH
                     AITMHQPLRCGEIKLYDLDINAWWVNGTPADCVKLGVETILSVQPDLIISGINDGENL
                     GTDIIYSGTVSAAIEGSMFNIPSIALSYASRCAADFSAPAEVAAQVIQQVLEHKGKNN
                     MLLNVNIPEIESIELLKGVKVTRLGVKKYRNNFEERKDPRGNSYYWLAGELVQDEIGE
                     DTDIYAVRNGYVSITPIHIDLSGYEEIKKISSWNISLNKNLTII"
     gene            5027..5665
                     /locus_tag="PKI50_08520"
     CDS             5027..5665
                     /locus_tag="PKI50_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03782.1"
                     /translation="MIQIDDAGSGSLVGGTCIGILRKETGEYYYEILPVQLFSETNFK
                     NKSYIYYTTKVVMNAFEKLSVKKEERINICQGYMFEDTRKFLARSGYTFTNTKIDEPL
                     QSLIEKTFENYTISLGLPMGFIAYTKYPFHFHRLLKWVYADFDNRTELCKTGWKSWQK
                     YSNAKVESYYDKLVKSSYICLKCGNRIPDNSNVKVIKFYSDRYNYVYVHKNC"
     gene            complement(5797..7194)
                     /locus_tag="PKI50_08525"
     CDS             complement(5797..7194)
                     /locus_tag="PKI50_08525"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HNT03783.1"
                     /translation="MARIKITETSLRDGHQSLIATRMRTDEMLPIIELMDKVGYHSLE
                     MWGGATFDTCLRFLDEDPWERLRLIKSKLKNTKLQMLLRGQNILGYKNYADDVVEEFV
                     RLSIKNGIDIIRIFDALNDLRNIETAVKAAKKYGVQAQGTVVYTTSPVHDIDLYVELA
                     KKVQEMGCDSLCIKDMSGILAPYYGYELIKALKKHISIPIQLHTHFTSGMTDMTYLKA
                     IEAGVDIIDTAISPFGLGTSQPPTEPMVATLKGTEYDTGLDLDLLCEIAEYFRKIKDK
                     YLANGILSPNVFNIDTNVLSYQIPGGMLSNLIAQLKQTNNMDKYNDVLKEVPRVREDL
                     GYPPLVTPSSQFVGTQAVFNVISGSRYKMIPNEIKDYVSGRYGRPSVPISEEIKKIII
                     GDEEVITCRPADLIEPQLESLKQEIKEYYEQEEDVLSYAVFPQVAMKFFKDRQAKKYG
                     IDSSLADKDNKTYPI"
     gene            complement(7313..7759)
                     /locus_tag="PKI50_08530"
     CDS             complement(7313..7759)
                     /locus_tag="PKI50_08530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT03784.1"
                     /translation="MMQIRKIKPEDISFVMELASKYGFSDFKLLSNIEHFLICEDGNT
                     KCGCGCLVPYKGKGYLSCVIVDEGHRRKKLGGAITKALLNIADLQGIEEVYAAGICKD
                     FLQAMGFEKYNGTDLEVDLLEAIGDIGISEYYRVLLKDYFKPCSHI"
     gene            complement(7731..8483)
                     /locus_tag="PKI50_08535"
     CDS             complement(7731..8483)
                     /locus_tag="PKI50_08535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT03785.1"
                     /translation="MAERLQKVMAEYGVASRRKSEEMICAGKVKVNGRLVTEPGFRID
                     KDRDVIEVDGDIIKSPEKKIYILLNKPTGYITSVKDQFGRPTVLDLLKGISSRVFPIG
                     RLDYDTEGLLILSNDGELTYRIAHPKHSIDKTYRALVRGEADKSDIKIFERGIAIEDY
                     VTAPAKMEIVRYSKGNSVIDITIHEGRNRQVRKMCSAIGHEVIRLRRIKIGDIGLGGL
                     KTGEWRYLRDSEIRYLKKLGGIWNDADKKNKT"
     gene            complement(8623..9756)
                     /locus_tag="PKI50_08540"
     CDS             complement(8623..9756)
                     /locus_tag="PKI50_08540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNT03786.1"
                     /translation="MKRNTAIVMAAVIAALSGFFAVQALQESSARAAVVMDVNSNRIL
                     YSKNMDEKMAMASTTKIMTTLVAIESGRLDEKVTISKRASYMEGSSIYLREGEVHTVN
                     DLLYAIMLRSGNDAATAVAEHIGGSAEGFAEMMNKKAREIGANNTRFANPHGLDAEGH
                     YTTARDLALITSYALKNPLFSTIASSKKKVVEGPPSENWDRVMINKNKMLWQFDGGDG
                     VKTGFTKKAGRCLVSSATRDGMRLVCVVLNCGPMWDESSALLEYAFKNYVKKRIVDKD
                     SIFKVVEVRNGKERFVGVKPAEDFDLALRTDRVENIKLEAKDLKAAQAPLKKGGHAGR
                     LEIYIDNNLLKTINLEYSESVESSSPFFYLKRILRDYIADAKQ"
     gene            complement(9846..10244)
                     /locus_tag="PKI50_08545"
     CDS             complement(9846..10244)
                     /locus_tag="PKI50_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNT03787.1"
                     /translation="MELKSAVDMIWENRKYLTDDPKEVLSHLNEEVAESLKALLRGEE
                     DRAKSELEDALSCILIALKVMDVDIEEAVQRQIIQMKKRHDKLMIFKKDKVEIYVDGT
                     LKGGWTIWGEDDIKEALKIAKEFGCKIDKE"
     gene            complement(10359..10769)
                     /gene="ytfJ"
                     /locus_tag="PKI50_08550"
     CDS             complement(10359..10769)
                     /gene="ytfJ"
                     /locus_tag="PKI50_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HNT03788.1"
                     /translation="MDQPVQSLMKTTMENIKNMIDVNTVVGDAVETKDGTVIVPVSKV
                     SFAFIAGGGEYTGATDKNEEDASKYPFAGGSGAGVTVQPIGFLATTNGQLRVIPVRYS
                     SAVDRIVDMVPDLINNMENYLKKKRHSEEDSLEM"
     gene            complement(10785..11408)
                     /locus_tag="PKI50_08555"
     CDS             complement(10785..11408)
                     /locus_tag="PKI50_08555"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HNT03789.1"
                     /translation="MLYAASGFIFLILILYLMPVEISTKVEKHNSNVKISLVLKTLYG
                     LFNVNTEIPAFKLDYENGKPVLKYKVEVANKERSRLLAGFSKLLTIEEGEGLYRIYKK
                     KKDIILPPLKYLTKKISVRNLYLKLKMGTGDAAATGLLYGTAWIAVGNIMALTRSYVN
                     IKEPRIIIAPIFDSARLSLDFSCIIRIKTGHIINAGIRVIPALITKL"
     gene            complement(11605..12159)
                     /gene="scpB"
                     /locus_tag="PKI50_08560"
     CDS             complement(11605..12159)
                     /gene="scpB"
                     /locus_tag="PKI50_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNT03790.1"
                     /translation="MHEGLGFVMDIREIKSIVEALLFVAGDAVPIKEICSIISIDETT
                     LRRIVMQMMDSFNEDNRGIQIIEVNNCYQICTKPEHYEYIERLVKPQSRQGLSQASLE
                     TLAIVAYKQPITKSQIDYIRGVKSDGCIARLAEKELIYEAGRLEGPGRAILYGTTDNF
                     LKLFGLRSIKELPALIELNGEEGA"
     gene            complement(12152..12883)
                     /locus_tag="PKI50_08565"
     CDS             complement(12152..12883)
                     /locus_tag="PKI50_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNT03791.1"
                     /translation="MAINVKLEAFEGPLDLLLHLIEKAEVDIYDIPIAEITDQYLHYI
                     NMMEIHDLEVSSEFIVMAATLLEIKSKMLLPKPKKEENITDEIDPREELVLKLIEYKK
                     YKEASLDLKDKLGIYSKVFYKPPEPIEDYIDDFASISNVSLDMLYMSFRNIILKNESR
                     RKNSYTEIYREVVTVEDKIRLINKLLAGKPSFYFDDLFIGCYNRYEIIVTFLAILELL
                     KRRSLLIEQEKNFSRILIKRRTKGA"
     gene            complement(12887..13879)
                     /gene="trpS"
                     /locus_tag="PKI50_08570"
     CDS             complement(12887..13879)
                     /gene="trpS"
                     /locus_tag="PKI50_08570"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNT03792.1"
                     /translation="MTRQRIMSGMRPTGTLHLGNYFGALENWVKLQNDYECFFSIVDW
                     HGLTTGYEDTSEFRNNTMEIAIDWLSAGLDPEKCTIFVQSNVKEIAELHLLLSMIVPL
                     SWLERCPTYKEQLSQLTEKNITTYGFLGYPNLMAADILVHKGNFVPVGEDQQPHLELA
                     REIARRFNYMYGDTFPEPQGKMTKIKVLPGLDGRKMSKSYDNTIAMSDSPETIREKIR
                     QMITDAEKIKKNDPGHPDICAVYAFHKVFSENTHQETYDVCKTGGIGCVQCKKVLAEN
                     VVQYMAPMYERRQKLLGNKEAILEILREGTRHAREEARETIKEVREKMKMLELE"
     gene            complement(13904..14560)
                     /locus_tag="PKI50_08575"
     CDS             complement(13904..14560)
                     /locus_tag="PKI50_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNT03793.1"
                     /translation="MTDLLSKFLYYLNLNFGISSLLYSLPGIILALTIHEYCHALAAY
                     AMGDNTAKQYGRLTLNPIAHMDPLGFLCLIVTRRFGWAKPVPVNDMNFRNRRKGMLLV
                     SLAGPMSNFITAMLIAAAYAVFYKSMGEITKGILQIAYIINLSIGAFNLLPIPPLDGS
                     KILDAFLNDRQRFFMRRYAMYSNMLILILIFSGIIGRVLGPVIMVMDWFINSVIGLFI
                     "
     gene            complement(14667..15863)
                     /locus_tag="PKI50_08580"
     CDS             complement(14667..15863)
                     /locus_tag="PKI50_08580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNT03794.1"
                     /translation="MYNSANINKLSLKLICYITIIAIAHFALNIPVCAENDMNLNCKS
                     AVLMDFGTGKIIYEQNSREKLPPASVTKVMTMLLIMEALDSGKISYEDKVTISPQASS
                     KNSKGTMLLLEAGEVRTVKELLYGIAVESANDACIAMAEHISGSEEEFVKLMNRRAKE
                     LGANDSNFVNPNGLPEDGHVTTAYDLAVLSRELLKHEKIFDFISQYMVKVYVGKKNNV
                     LRELVNKNKMVRFYNDVDGIKTGWTEEAGYCISVTAKRNNLRLISVVMGSPKVDIRNK
                     EARKLIDYGFANFNSLIIAKKGDKIQEVNVSKGSRLKVNALAESDVSVLLNKGEEKSI
                     VKKAELPEALKAPVKKGEKIGELVISINDKEIGRHNLISDADVNKSTFLNNLRNAIIY
                     WFGNSN"
     gene            complement(15992..17293)
                     /locus_tag="PKI50_08585"
     CDS             complement(15992..17293)
                     /locus_tag="PKI50_08585"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HNT03795.1"
                     /translation="MRMYDIITKKRNGEELTREEIQFFINGYTDGSIPDYQAAALLMA
                     VYFKGMSKRETAELTMAMVNSGETVDLSKIAGVKVDKHSTGGVGDKISLIVIPLAASL
                     GIPVAKMSGRGLGHTGGTIDKLESIPGFRTELTREEFIGNVNKYKMAIAGQSGNLVPA
                     DKKLYALRDVTATVDSIPLIASSIMSKKIASGAECIVLDVKAGSGAFMKSVEEAAELA
                     GTMVEIGKSLNRKTVAVVTDMSQPLGHEVGNSNEIIEAIDVLKGKGSEDERQVALTIA
                     SYMAVLGGAFEEFEKAHSALEEVIRSGKAIDNLKLLIQIQGGDPDVVDNTEKLPKAKY
                     HIEVRSKKSGYVDSIDAESIGISAMLLGAGRKTKEDKIDFSAGISMKKKVGDKVAVGD
                     ILCVLHTNLGNTAEAEKIALEAYGIGSTEKKIVKYIYEVIQ"
     gene            complement(17293..18117)
                     /locus_tag="PKI50_08590"
     CDS             complement(17293..18117)
                     /locus_tag="PKI50_08590"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNT03796.1"
                     /translation="MELRERIEKSTDFIRENTGFEPETGIVLGTGLGSLADYIDKEAV
                     IDYKGIPEFPVSTVFGHEGRLILGRLEGKKVIAMQGRFHYYEGYTMQEVTIPVRVMKQ
                     LGIKLLVASNACGGLNPNFAAGDIMIITDHINFMGTNPLIGPNLEEFGPRFPDMSQVY
                     DKELVELLEKIAASQGIRVHKGVYGAVSGPNYCTKAELSMMIKLGADTVGMSTVPETI
                     AARHCGLKVAGVSCITDMAIPDTMTAPSHEEIVKVAESVKPRFVSLVKQFIKEVRL"
     gene            complement(18142..18966)
                     /locus_tag="PKI50_08595"
     CDS             complement(18142..18966)
                     /locus_tag="PKI50_08595"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNT03797.1"
                     /translation="MKENLLSLINEAGDYLTERAGAAPEAALILGSGLGDLAEEAENK
                     RIVGYKEIPGFPVSTVQGHKGRLVFGTMGGKRVVFMQGRFHYYEGYSMKEVVFPIWVF
                     NSLGVKKLIVTNAAGGINTSFKPGDLMIIKDHINYTNNNPLIGANIEAFGPRFPDMSA
                     AYSKELADIIRECAASEGIKLREGTYLYLTGPSYETPAEIRAFRILGADAVGMSTVPE
                     VLAANHCGIKTAGISCITNMAAGILDTPLNHKEVMETADRVKESFSKLIRGAMERI"
     gene            complement(18985..20160)
                     /locus_tag="PKI50_08600"
     CDS             complement(18985..20160)
                     /locus_tag="PKI50_08600"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNT03798.1"
                     /translation="MVSRVILIVLDSVGIGELPDADMYGDSGSNTLGCLVRNVKDFKL
                     KNLEALGLGNIDRSIGLQIAENPTGSFGRAKEVSPGKDTTTGHWEIAGVTLSRPFPTF
                     PEGFPKEIVEAFERAIGVKTLGNEAASGTEIIARLGDKHVETGYPIIYTSADSVFQIA
                     AHEEVIPFQKLYEICEKARALLKDEYAVGRVIARPFLGKSGSYKRTSNRRDFSLKPIK
                     KTMLDIIVENGMNVAAVGKIEDIFAGEGISEAVHTKDNTDVVDKTLEYMSEDKTGLIF
                     SNLVDFDMLYGHRNDAEGYAGALMAFDERLPEIFAAMKDSDVLIITADHGCDPTTDST
                     DHSREYIPILVYGRHVRQGTDIGTREGFSDIGATVLDLLGLPIEIDGKSFKTEIYKI"
     gene            complement(20237..21127)
                     /gene="xerD"
                     /locus_tag="PKI50_08605"
     CDS             complement(20237..21127)
                     /gene="xerD"
                     /locus_tag="PKI50_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNT03799.1"
                     /translation="MILMSFIELFLDYLINNKELSANTLESYKRDIRQFEVYLREHAL
                     SLEKVTKTIIITYLIALQKAGKATSTISRNLASIRCFYQFMQNERYLDMDPSANLESP
                     KVEKKLPSVLTKKEVELLLEQPVPADAKGARDKAMLELLYATGIRVSELISLNIDDVD
                     LNSGLIICRNGEINSRSIPLGNVAVKYIKTYLNDFRKKLCTNENHKLLFVNFHGQKMT
                     RQGFWKIIKYYTAKANIKKSITPHTLRHSFAVHLIENGADLQAIQEMLGHSDISTTQV
                     YSKINKSRIKEVYSKTHPRA"
     gene            complement(21221..>21516)
                     /locus_tag="PKI50_08610"
     CDS             complement(21221..>21516)
                     /locus_tag="PKI50_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNT03800.1"
                     /translation="FLTENVEGNGFLLSLGSVLPQNLIFVPVIIVMGVIGFNYSLWAL
                     RYKCFKKSAAAPRLFAAYALTMLVLFILLVAGSIVEAYITSHIVKAIVPFIKY"
BASE COUNT         5525 a         5262 c         3725 g         7004 t
ORIGIN      
        1 gaggtcgggt ggaaagtttc gtcaaagata tcttccccac gtcggtattt gcattctctt
       61 tatatacaag gcagtattct gtaggatgca taaccaagca agagcttgta tagtatagca
      121 gggcacactt ttatgcattt tggtaacaag cctttgatat ggctttggta aaaagttcgt
      181 gaagccggca tggacgccgg cttaggtgcc acgggtcatg gaggaccttt tggcaccgtt
      241 agaacttttc caaaggcata gcagtaggcc tgtcaaaatc ataatccgtg tgaatgctat
      301 actatactgt ctcttgcgga gggttatgca aacttcagat atgtcgcatt cttcttcgat
      361 tgcgaatcaa atatgaatga aaaaatcctg ctttgtacaa agcaggattt ttattactca
      421 tccttatccg ccgaaaaaat attgtatttg ttccatatat cctccagctt tgcaaaggaa
      481 tcggatatat catgcttctg cttgattcct accgctacaa tcagtgcatt cagcaagctc
      541 ataggagcaa ctattgagtc cacaaaagac tccatattgc tttttgcaat aagagtataa
      601 tctgcattag cgcacagagg cgaaagtatg ctgtctgtta ttgctattgt gtttgctcct
      661 ctggattttg catattcaaa ggcctttaat gttcttgtag aataccttgg gaatgtaatt
      721 ccaattacca gatcatcttt gccgattctg aaaagctgtt caaaaatatc tccgatagta
      781 tatgtcacaa catgaacatt atcccttaca aaattgagat aaaaacccag aaactctgaa
      841 agtgtattgg aactcctcaa gcctattatg taaatgcttt ttgcttcaat tatggctctt
      901 acagcttgtt caaaggctga gctgtctatc tcttccattg ttccccttat gttttcgata
      961 tcagacttga ggatagtctt taacacactt gcctgattgg tataatccga actcatttca
     1021 attctttgaa ctgttgtaag cttggttctt accatttcct gcagttgctt ctgcaattgg
     1081 ggatatcctt tatatccgac tgtaatcgca aaccttacaa ccgtcgattc acttacttcc
     1141 accacttccc caagcttggc ggcagtcata aatgcagcct tatcataatt tttcattatg
     1201 tattgtgcaa ttatcttctg ccctttactc agcttttgaa attttgtctg catgactttc
     1261 atcaagttga tattttcact catactgcct atctccattt acatcagttt atttgcaatt
     1321 tcatatcctt ttgcaagtgc tttcctgttg agttcctccg tacctttcgg aaccctttca
     1381 agaacagctt tttccaagga ttcctttgat ataacctttg ttatctcaac caatactcca
     1441 agggcaacaa tattggttac catcggcttg cccagatctt taacggctga atcaattata
     1501 ggaatgctgt atatcttaat attccctgat tttacagcat ttacggagct gtctactata
     1561 agtatcccat cttccttgag gccttcgttg tattcatcat aggctttttg cgtcaatgac
     1621 aacagtatat cacaatttct tacttttgga aaatctatct cttcacttga tattataacc
     1681 tctgatttgc ttgctccgcc tcttgcctcc gggccgtagg attgtgtctg taccgaattc
     1741 ttaccgtcat ttattgcacc ctccgccaga ataattcccc ccagaagcag tccctgccct
     1801 cctgagccgc ttagtctgat ttcatatctt tccatcactg actctccttt ttcaatctat
     1861 cgattatctt ttgatactca ttggtgtatt ccggttccgg actgttcttt aactcaccaa
     1921 tgaggaattt gccttccaat gcttcaggag acatctttgc cgcagccgat atgttgattg
     1981 cactgttctt ctgccattcc atcatactta ctgcactgcc ctttttattc tttctgccgt
     2041 agtaagtcgg gcacatgctt atagcttcta tcagggaaaa cccctcgttg tttatagcat
     2101 tctgcaccaa ggatgtgacc aggcttgcat ggtaagctgt ggctcttcct acataagtgg
     2161 ctcccgctga ggcggccagc ttgcatatgt caaaggcttt atcaatattt ccgtaaggag
     2221 ctgtagttcc tttatcatat ttcggtgtgg taggcgaata ctggcctcct gtcataccgt
     2281 atatactgtt gttgtacact atagctgtta ttcctatatt tcttctgcat gcgtgaataa
     2341 gatgattgcc tccgatagct gtcgcatctc cgtctcccat tattacaata accttgattt
     2401 ccggctttgc catttttata cctgttgcaa aagccaatgc tcgtccgtga gtggtatgaa
     2461 gcgtattaaa gtcaagatat cccggtgctc ttgatgaaca gcctatgcct gacacaatac
     2521 agattttgtt tttatcaata cctgacttct ctattgcgga agctattgat cttgttatta
     2581 tcccatgacc gcagccgggg caccatatct gcggaagctt ttctgttcta aagtaattct
     2641 caactaatac actgggcata ttagagtacc tcctcaatct tactgactat ttcctcggga
     2701 taaatgatct cgcagctgac cttgttcaat ttttcaattt ttgcattaca gccgcatatt
     2761 ctttcaactt ccaggaccaa ctgtcctaaa ttcatttcag gtactacaat ggtttttacc
     2821 ttggctgcca gttctctcaa ccgcttttca gggaaaggcc agatagtgat tggcctgaaa
     2881 gtgcctactt ttatgcctct tccccggaga atattcatcg ctgattttga tgacctggct
     2941 acgcagccgt aggataatat gagcacttca gcatcttcgg tgttttcttc ctcccatgac
     3001 tcgatttcat ctctatgtat ttctatttta tttgaaagcc ttcttatgag cttatcggct
     3061 actgcagtgc tgttgctggg aaaacctgtt tcatcatgca ccaatccggt tacatgatat
     3121 ctgtagccgg taccgaagtc cgccatcggc ggcaccaggt cgttatcagc ttcatacggc
     3181 aagtattcct cgctgctgca gtccggcctc tttctatcta tgattactat ttctgaaggg
     3241 tcaggtaatt gaactttttc tcgcatatgt ccgattactt catccataag aagtataacc
     3301 ggcgtcctgt atttttctgc aaaattaaaa gcctttattg taaggtcaaa ggtttctctg
     3361 actgaggaag gagtaagggc aataacagga tgatccccat gagtacccca acgggcctgc
     3421 atgatttcgc cctgagccgg ggaagtaggc attcccgtac tgggcccccc tctctggaca
     3481 tttactatta cacaaggtac ttcagctatt gaagcataac ctatgttttc ctgcttaaga
     3541 gaaaaaccgg ggccgctggt cgccgtcatg cttttggctc ccgtaaggga agctcctatt
     3601 atggcggcca ttcctcctat ttcgtcctcc atctgcataa atcttccgcc gaccaaaggc
     3661 agcctttctg cggataattc agctatttca gttgaaggtg taatgggata accggcataa
     3721 aaccgcattc cggcagcaat tgcaccttcg acgcatgctt catttccctg catcaatcta
     3781 accttgcttt tcattttttt tcctcctcag atatattgca tagtcaggac atctaagctc
     3841 acacattccg cactgtgtac acttatcaac atcgaccatt acaaccttat cattcttaag
     3901 ctccaatgct tttaccgggc aaaaagcgac acagatgccg cagcctttac accaggcagg
     3961 attgattact actttttcat tttgtatttc tcctgctttg atatcctcca ttttcatttt
     4021 tttgacctcc aaaaattcat tagataatgc agcttttatt tcatttattg tatttacatt
     4081 gggaattctg caactttccc ttcaattttt agtatatatt ataatattct catttgcaat
     4141 aattaatgcc aaagaaattt catattattg tcaggttttt attcagtgag atattccaac
     4201 tgctgatctt ctttatttct tcatagcctg aaaggtcgat atgtataggt gttattgaca
     4261 cgtacccgtt tcttaccgcg tatatatccg tatcctcacc gatttcatcc tgtacaagtt
     4321 caccggccag ccaataatag ctgtttccgc ggggatcctt cctttcctcg aaattattcc
     4381 tgtacttttt aactccaagc ctggttacct ttacaccctt cagtaattca atactttcaa
     4441 tttcaggaat attaacattt aacagcatat tgttttttcc cttgtgttcc agaacttgct
     4501 gtatcacttg ggcagcaact tccgcaggcg cggaaaaatc ggctgcgcag cggcttgcat
     4561 aggataaagc tattgagggt atgttgaaca ttgaaccttc aatggctgca gataccgttc
     4621 ccgaatatat tatgtccgta ccaaggtttt caccgtcatt aatacctgaa attatcaaat
     4681 caggttgtac ggacaatatt gtctcaactc ccagcttgac acaatccgca ggtgtgccgt
     4741 ttacccacca tgcattaata tccaggtcat acagctttat ttccccgcat ctcagcggtt
     4801 ggtgcatggt aattgcatgg cctatggcgc tttgctcctt atcaggggca acaacagtga
     4861 ccttgcccaa tgctgccatt ctctttgcca gttcatataa tccctttgaa tatattccat
     4921 catcgttgga tattagtatg tttaacattt gtaccctccc acttcatgtt taataatttt
     4981 ttcttttaca tatactaaag ccactttgta ctgtgcaggt gataaaatga tacagataga
     5041 tgatgcggga agcggcagcc tggtaggcgg cacctgcatc ggcatcttgc gaaaggaaac
     5101 aggagagtac tattacgaaa tactccccgt tcaattattc agcgaaacca actttaaaaa
     5161 caagtcatac atatattata caacaaaagt tgtaatgaat gcttttgaaa agctctcggt
     5221 aaaaaaggaa gagagaataa atatatgcca gggctatatg tttgaagaca caagaaaatt
     5281 tcttgcccgg tcaggctata ccttcacaaa caccaaaata gatgaaccac ttcaatcact
     5341 tatagagaag acctttgaaa actatacaat ttccctcggc cttccaatgg gttttatagc
     5401 ttatacaaag tacccctttc attttcacag attactgaaa tgggtatatg ctgactttga
     5461 taaccgtacg gaactttgta aaacaggatg gaaaagctgg cagaaatata gcaatgccaa
     5521 ggtggaatca tactatgaca aattggttaa aagcagctat atatgcctga aatgcggcaa
     5581 tagaatacct gataattcaa atgtaaaggt cataaaattc tattccgata ggtacaatta
     5641 tgtatatgtg cataagaatt gctgaagggg ggagtgagac acggggagac acggggagag
     5701 acacggggac gtttcacgtg tccctccagg acaggcgaaa cgtccccgtg tctcaacgtc
     5761 cccgtgtctc cccatgtctc cccgtgtcta cttatattaa attggatagg tcttattatc
     5821 tttatccgca aggctgctgt ctataccgta tttctttgcc tgcctgtcct taaagaattt
     5881 catggcgacc tgcgggaaaa cggcatagct caaaacatcc tcctcctgct cgtaatactc
     5941 cttaatttcc tgcttcaggg attccaactg cggctcaatt aaatccgcag gcctgcaggt
     6001 tataacttcc tcgtccccta ttattatctt ctttatttcc tcggaaatcg ggacgcttgg
     6061 ccttccatac ctgccgctta cataatcctt tatttcattt ggtatcattt tatacctgct
     6121 gcccgaaata acattaaaga ctgcctgggt gcctacaaac tggctggaag gcgttacaag
     6181 aggaggatag ccaagatcct ccctcaccct tggcacctct ttcagcacat cattatattt
     6241 atccatgtta tttgtctgct tcaactgggc tatcaagttg gacagcatcc ctcccggtat
     6301 ctggtatgac aacacattgg tgtcaatatt gaatacattg gggctcagta tcccattggc
     6361 caaatactta tcttttatct ttctgaagta ctccgcaatt tcgcacagta aatccagatc
     6421 caggccggta tcgtattccg ttcccttcag cgttgccacc attggttccg ttggcggctg
     6481 tgaggttccc agcccaaaag gagatatggc tgtatctatt atatctactc ctgcctctat
     6541 agcctttaaa taggtcatat ccgtcattcc gctggtaaag tgcgtatgaa gctgtatcgg
     6601 tatgcttatg tgctttttca gcgctttaat aagctcatat ccgtaatagg gagcaagtat
     6661 accggacata tccttgatgc aaagggaatc acaccccatt tcctggactt ttttcgcaag
     6721 ctccacatac aagtctatat catgtaccgg gcttgtagtg tatacaactg tgccctgggc
     6781 ctggacgccg tacttttttg ctgctttaac cgctgtttca atatttctca aatcattcag
     6841 ggcatcaaat attcttataa tgtctatgcc gttcttgatt gaaagtctta cgaattcttc
     6901 aaccacatcg tcggcatagt tcttatatcc cagtatattc tgtcctctga gaagcatttg
     6961 cagctttgta ttcttaagct ttgacttgat taatcgaagt ctttcccatg ggtcttcgtc
     7021 caggaatctg aggcatgtgt caaaggtagc tcctccccac atttccaatg aatgatatcc
     7081 caccttatcc ataagctcga tgatcggcag catttcatcc gtcctcatcc ttgtagcaat
     7141 aagtgattgg tgtccatccc tcaatgatgt ttctgtaatt ttaatccttg ccatatttat
     7201 ttcctcctgt atattttctg caattattga atatgctgta ataaaaataa ttattattaa
     7261 tatagcacat tattaaataa aaaaaaatgt ttctttacaa aaaagttact ttttatatat
     7321 gcgaacaagg cttaaaataa tcttttagaa gcaccctata gtattctgat atgcctatat
     7381 cgccaattgc ttctaataag tcaacctcta aatcagtgcc attatacttc tcgaagccca
     7441 ttgcctgcaa aaagtccttg cagattcctg ctgcgtatac ctcctcaatt ccctgcagat
     7501 cggctatatt aagcaatgct tttgttatcg cacccccaag cttttttctt ctgtggcctt
     7561 catccactat aacacagctc agatatcctt ttcccttata cggaaccaga cagccgcatc
     7621 cgcatttggt attcccatcc tcgcatatca ggaaatgctc tatattagac agcagcttga
     7681 agtcagaaaa tccgtatttt gaagcaagct ccattacaaa gcttatgtcc tcaggtttta
     7741 tttttcttat ctgcatcatt ccatatgccc cccagttttt ttaaatatct tatttctgaa
     7801 tccctcaggt atctccattc gccggttttt aaccctccca gcccgatatc tcctattttt
     7861 atccgccgca gccttattac ctcatgccct atggcggaac acattttcct gacttgcctg
     7921 tttctgcctt catgtatggt aatatcaatt acgctgttgc cttttgagta tcttacaatt
     7981 tccatctttg cgggagcagt aacataatcc tcgatagcaa tacctctttc aaagatttta
     8041 atatcactct tgtccgcttc tccgcggacc aaagctctgt aagtcttatc gatactgtgt
     8101 ttagggtggg caattctata tgtaagttca ccgtcattac tcaaaatcaa aaggccctca
     8161 gtatcataat ccaggcgtcc aataggaaag accctggagc ttattccctt taacaggtct
     8221 aagacagtgg gccttccgaa ttgatccttt acgctggtta tatatccagt aggtttgttc
     8281 agtaaaatat aaattttttt ctccgggctt ttgatgatat ccccgtcaac ttctatcaca
     8341 tctctgtctt tatcaatcct gaagcccggt tccgtaacca acctgccgtt gaccttcacc
     8401 tttccggcac atatcatttc ttcactttta cgcctggaag ccactccgta ctccgccatt
     8461 actttttgca gtctctccgc catttccccc tacccctcac aattaatcat taaatatcag
     8521 ccaaaaggta aaacctttga gatatgctta atttaaggta atctgccatt atataaacct
     8581 tcttgataag catcaagcct attttcccat aaaagatatt tcttactgtt ttgcgtctgc
     8641 tatataatcc ctcagaattc gtttcagata aaaaaaaggg ctgctggact ccacactttc
     8701 cgagtactca agatttattg tcttcaacaa attattgtca atgtatattt caagccttcc
     8761 cgcatggccg ccttttttta atggggcctg cgccgctttc aaatctttag cttccaattt
     8821 aatattttca accctgtctg ttctcaacgc caggtcaaaa tcctccgcag gcttgactcc
     8881 gacaaacctt tctttcccgt tccttacttc cacaacctta aaaatgctgt ccttatctac
     8941 aattctcttt tttacataat ttttaaatgc atattccaga agagccgatg attcatccca
     9001 catcggcccg cagtttaaaa ctacacaaac cagccgcatt ccgtctcttg tagcggaaga
     9061 taccaggcat cgccctgctt tttttgtaaa acctgttttt actccgtctc ctccgtcaaa
     9121 ctgccagagc attttgtttt tattaatcat tactctatcc cagttttcac ttggagggcc
     9181 ttccacgacc ttttttttgc tgcttgctat tgtcgaaaac agagggtttt tcaaagcata
     9241 agaagtaatg agagcaagat cccgggcagt ggtataatgc ccctccgcat ccaggccgtg
     9301 gggattggca aaccttgtat tatttgctcc aatctcccgg gcctttttat tcatcatttc
     9361 cgcaaaaccc tctgccgacc cgccgatatg ctctgctaca gccgttgcgg catcatttcc
     9421 cgaccggagc attattgcgt acagtaaatc attgactgta tgcacttcac cttcccgcag
     9481 gtatatggag gagccttcca tataagaagc tcttttgctt attgtaacct tttcatctaa
     9541 tcttccgctt tcaatagcta caagagttgt cattatcttt gtagtactgg ccatggccat
     9601 cttttcgtcc atgtttttgg aatatagtat cctgttggag tttacatcca tgactactgc
     9661 agcgcgggca ctggattcct gcaaagcctg cactgcaaaa aagcctgata atgcagcaat
     9721 aactgctgcc atgactatag ccgtatttct tttcatcaaa tcactccgaa ataataaatg
     9781 atttacatct caatattaaa tgcatacata tattattaac agaatgatat atttatataa
     9841 ttccattatt ccttgtcaat tttgcagcca aattccttgg caatcttgag cgcttccttt
     9901 atgtcatcct ctccccagat agtccagcct ccttttaatg ttccgtctac atatatctct
     9961 accttgtctt tcttaaaaat catcagcttg tcatgtcttt tcttcatttg tataatttgc
    10021 ctttgtactg cttcttcaat atcaacatcc attaccttca gcgcaataag aatacatgag
    10081 agagcatctt ccaattcact cttagccctg tcttcctcac ctctgagcag ggctttcaat
    10141 gattcggcaa cttcctcatt caaatggctc aatacttctt taggatcatc agtcaagtat
    10201 tttctgtttt cccatatcat atcaactgca ctttttaact ccatataatt acctcccgat
    10261 aaagcatatc attccttaag gataataatc aaaactttct atattatcaa gtctattata
    10321 gcatttttat gtaaaaaagg aaggagttaa actccttctt acatttcaag gctgtcttct
    10381 tcagaatgcc ttttcttttt taaataattc tccatattat ttataaggtc aggcaccata
    10441 tctactattc tgtcaacggc actgctgtac ctgacgggta tgaccctcag ctgtccgttt
    10501 gttgtagcca gaaaacctat gggctgtacc gttacccccg ctccgcttcc tcctgcaaaa
    10561 ggatactttg aagcatcctc ttcgttctta tcggttgctc ccgtatattc tccgcccccg
    10621 gctatgaatg caaaggatac ctttgacact ggtacaataa cggttccgtc ttttgtttca
    10681 acagcatcac caactaccgt attaacatct atcatgttct tgatgttttc cattgtagtt
    10741 ttcatcaagc tttgaacagg ctgatccaag ctaaacacat ccttctataa tttagttatc
    10801 aatgcaggta ttaccctaat tccggcattt ataatatgac ctgttttaat gcgaattata
    10861 caactgaaat caaggctcag cctggcactg tcgaatattg gagcaattat tatcctgggc
    10921 tccttaatgt taacatagct tcgggtaagt gccattatat tgcctaccgc tatccatgct
    10981 gttccataca acaatcccgt tgctgcggca tcaccggttc ccatttttag tttcaggtaa
    11041 agatttctaa cagaaatttt ttttgttaaa tattttaaag gtggtaaaat tatatccttt
    11101 ttctttttat atatcctgta taagccttct ccttcttcaa tagtaagaag cttgctaaat
    11161 ccggccaata acctgcttct ttccttattt gctacttcaa ccttatactt caatacaggc
    11221 tttccgtttt cataatccag cttaaatgca ggaatttcgg tattgacatt gaacaagcca
    11281 tataaggtct tcagcaccaa ggaaatcttc acattgctat tgtgcttttc aaccttcgtg
    11341 cttatttcta cgggcatcaa atacaatatc aatattaaaa atatgaaccc ggaagcagca
    11401 tacagcatca caattcctcc aagcatgaga cacggggacg tttcgcgtgt ctcatttcag
    11461 tgagacagga gaaacgtccc cgtgtctcta ctcttttcca atctgtatat ctttctcaaa
    11521 aaataaaaaa aaatacatca tctgatgtat aggataaaga caaagtgcag aaggttaaca
    11581 aggctttgaa atgcacacag ccctctaagc tccttcttct ccattaagtt ctatcagagc
    11641 aggcagctcc ttaatggatc tcagcccgaa cagcttaagg aaattgtctg tggtcccgta
    11701 cagtatcgcc ctgcccgggc cctccagcct tcctgcctca tatatcaatt ctttttccgc
    11761 taatcttgcg atgcatccgt cggattttac ccctctgata taatcaatct gagacttggt
    11821 tatcggttgt ttatatgcaa ctatcgcaag tgtctcaagg gatgcctgag aaaggccctg
    11881 gcggctttgg ggcttgacca gcctttctat atactcataa tgctccggct ttgtgcaaat
    11941 ctggtagcag ttgtttacct caataatctg gattcctctg ttatcttcat taaagctgtc
    12001 catcatctgc attactattc ttctgagtgt agtttcgtca atactgatga tgctgcatat
    12061 ctcctttatg ggaacagcat ctccggctac aaacaaaaga gcctctacaa tcgactttat
    12121 ttctcttata tccataacaa aacccaaccc ttcatgcacc ttttgtcctt ctcttaataa
    12181 gtatccttga gaagttcttt tcctgttcaa taagcaggct tcttcttttc agcagctcaa
    12241 gtatcgccag gaaagtgaca attatctcgt acctgttgta acatccgata aacaagtcgt
    12301 caaagtaaaa tgacggtttg ccggcaagca atttgtttat cagccttatc ttgtcttcta
    12361 cggtgacaac ctccctgtat atttccgtgt agctgttttt ccttcttgat tcattcttca
    12421 aaattatatt tctgaagctc atatacagca tgtccaagga aacattggaa atggaggcga
    12481 aatcatctat gtaatcctct atgggctcgg gaggcttgta aaaaaccttg gaatatatgc
    12541 ccagcttgtc ttttaaatca agagaggcct ccttatactt tttgtattca ataagcttta
    12601 gaacaagctc ttcccttgga tcgatctcat cagttatatt ttcttccttc ttcggcttcg
    12661 gcagcagcat ctttgatttt atctccagca aggttgcggc cataacaata aattcgcttg
    12721 aaacctcaag atcatgtatc tccatcatat ttatataatg gaggtactga tccgttatct
    12781 cagcaattgg tatatcatag atgtctacct ctgctttttc aatgagatga agcagaagat
    12841 ccaaaggacc ttcaaaggct tccagcttaa catttatcgc catgtgttac tccaattcta
    12901 acattttcat cttttctctt acttccttaa ttgtctccct tgcctcttct ctggcatgac
    12961 gggttccctc tctcagtatt tccagaatgg cctccttatt gccgagcagc ttctgtcttc
    13021 tctcatacat gggggccata tactgaacta cattttctgc aagaaccttc ttgcattgta
    13081 cgcagcctat acctccggtc ttgcatacat cataagtttc ctgatgtgta ttctcggaaa
    13141 aaaccttatg gaatgcatat accgcgcata tgtccggatg tcccgggtca ttcttcttta
    13201 ttttttcagc atccgtaatc atttgcctta tcttttccct gatagtctcg ggagaatccg
    13261 acattgcaat tgtattgtca tagcttttgc tcatttttct tccgtcaagg ccgggaagca
    13321 cttttatttt cgtcattttc ccctgaggtt cagggaaggt atccccgtac atataattaa
    13381 accttcttgc gatttccctg gcaagttcca aatgcggctg ctggtcctca ccgaccggca
    13441 caaaatttcc tttgtgaaca agtatgtcag cagccatcag attaggatat ccgagaaaac
    13501 cataagttgt tatgttcttc tcggtaagct gtgaaagctg ttctttatag gtagggcatc
    13561 tttcaagcca tgaaagcggt acaatcattg acagcaaaag atgcagttca gcaatttcct
    13621 tgacattgga ctgtacgaat atggtacatt tttccggatc caagcctgcg ctcagccaat
    13681 ctattgcaat ttccattgtg ttgttcctga attcactggt atcttcatag cctgttgtta
    13741 agccatgcca atcaacaatg gaaaaaaagc attcataatc attctgcagc tttacccaat
    13801 tctccagtgc tccgaaataa ttgcccaggt gaagagtgcc cgtaggcctc ataccgctca
    13861 taattctctg tcttgtcatt ttaattacct ccagtgctat cttttaaata aacaatccta
    13921 tgacagaatt aatgaaccaa tccataacca ttatgacagg ccccaatact ctgcctatga
    13981 ttcccgaaaa aattagtata agtataagca tatttgaata catggcatat cttctcataa
    14041 aaaacctctg tctgtcattc agaaaagcat caagtatttt tgagccgtcc agaggcggta
    14101 tcggaagcag attaaaggcg cctatgctta ggtttattat atacgcaatc tgcaaaatac
    14161 ctttggttat ctctcccatg cttttgtaaa aaacagcata agccgcggca ataagcatgg
    14221 cagttatgaa atttgacatc gggcccgcaa gggatacaag cagcattccc tttcttcggt
    14281 tacggaaatt catatcattt accggcacag gctttgccca gccgaatcgc ctggtgacta
    14341 tcaggcataa aaaacccaaa ggatccatat gggcaatggg atttaacgta agccttccat
    14401 actgtttggc tgtattatcc cccattgcgt aagccgccag agcatggcag tattcatgta
    14461 tcgtcaatgc gagaattatg cccggcagac tgtataataa agatgatatg ccaaagttca
    14521 agttcagata gtataaaaat tttgataaca aatcagtcac ggaataactc ctttgtatgg
    14581 aaaaataata tatctaatat attttaatac atttgcgcta tttatacaat ataaagaaaa
    14641 aacccggcat agccgggaag tgtatgtcag ttcgaatttc cgaaccaata tattattgca
    14701 tttcttaagt tattgagaaa ggtagatttg tttacgtcag cgtcgctgat caaattgtgc
    14761 ctcccaatct ctttatcatt aatgcttatt accagctctc cgatcttttc gcctttttta
    14821 acgggtgctt tgagagcctc aggcagctct gcttttttta ctatactttt ctcctcaccc
    14881 ttgttcagca gcaccgaaac atcgctttcc gccaatgcat ttaccttcag cctactgcct
    14941 tttgatacat tcacttcctg tatcttatca ccctttttcg ctattatgag gctgttgaaa
    15001 tttgcaaatc cgtaatcaat aagctttcgg gcttctttat ttcttatatc caccttcgga
    15061 gaacccatta ccacacttat aagccttaaa ttattccttt ttgcagttac ggaaatgcaa
    15121 taccccgctt cctcagtcca tcccgttttt ataccgtcca catcgttgta gaatctgacc
    15181 atcttgtttt tatttaccaa ttctctcaat acgttgtttt tctttcctac atagaccttg
    15241 accatatact gtgatatgaa atcaaatatc ttttcatgct ttaaaagctc tctggacaat
    15301 acagccagat cataggccgt tgtcacatgc ccgtcctcag gaaggccgtt ggggtttaca
    15361 aaattgctgt catttgctcc cagctctttt gctctcctgt tcataagctt cacaaattcc
    15421 tcttcacttc cggatatatg ctcagccatt gcaatgcagg catcatttgc cgactccacg
    15481 gctatgccgt acaagagttc ttttaccgtc ctgacttcac cggcctccag cagcagcatt
    15541 gtacccttgc tgtttttgct ggaagcctgg gggcttattg taaccttatc ctcatagctt
    15601 atctttccgc tgtccaatgc ctccataata aggagcattg tcattacctt tgtaacgctg
    15661 gcagggggta atttttcgcg ggaattctgt tcatatatta ttttgcccgt gccgaaatcc
    15721 ataagtaccg ctgatttaca gttaaggttc atatcatttt ctgcgcatac agggatattt
    15781 aatgcaaaat gggcaattgc aattatggta atataacata tcagcttaag ggataattta
    15841 tttatatttg ccgaattata cataacaatc ctcctacttg atttgtttgc ttttatattg
    15901 ccctttagaa gatttcgtta tgcaatgcaa atatattgca ttaagcttaa agaatgacag
    15961 aaaggcagcc caccagcttt ctattcctta attactgtat tacctcatat atatatttga
    16021 caatcttttt ttcggtactg ccaatgccgt atgcttccag tgcaattttc tcagcttcag
    16081 cagtattgcc caaattagta tgaagcacac acagaatgtc gccgacggct actttatccc
    16141 caaccttctt tttcatgctt attccggcag aaaagtctat tttgtcttcc ttggtttttc
    16201 tgcccgcacc cagaagcatt gccgaaatgc ctatgctttc cgcatcaatt gaatctacat
    16261 agccgctctt ttttgacctt acttctatat gatatttggc cttcggcaac ttttcagtat
    16321 tgtcaaccac atccgggtct ccgccttgaa tctgtatcag cagctttaaa ttatcaatag
    16381 ccttgccgct cctgattact tcttccaatg cggaatgtgc tttttcaaat tcttcaaaag
    16441 cgcctccaag taccgccata taggaagcaa tggtcaaagc cacttgtctt tcgtcctccg
    16501 aaccctttcc cttcaatacg tcaatggctt caattatttc attggaatta cccacttcat
    16561 gccccagggg ctgactcata tccgttacta ccgctactgt tttcctgttc agggacttgc
    16621 ctatttcaac cattgtccca gcaagctctg ctgcctcttc aacagatttc ataaaagcgc
    16681 ccgaacctgc ttttacatca agaacaatac attcggcacc ggaagcaatc tttttactca
    16741 taattgagct tgcaatcaaa ggaatactgt ctactgttgc cgttacatcc cttaaggcat
    16801 acagcttctt atcggcgggc acaaggtttc ccgactggcc cgcaatagcc attttatact
    16861 tgtttacgtt cccaatgaat tcttctcttg tcagttccgt cctaaaaccc ggaatggact
    16921 ccagcttatc tatggttccg ccggtatgcc ccaaccctct gccgctcatt ttggcaacgg
    16981 gaatacccag ggaggcagcc agagggatga caatcaaact gattttatct cctacccctc
    17041 ccgtggaatg tttatcaacc ttcacacctg caattttcga aaggtcaacc gtctctcccg
    17101 aatttaccat agccatagta agctccgctg tctctctttt gctcattccc ttaaagtaaa
    17161 cagccataag aagtgccgct gcctgataat cagggattga gccgtcggtg tacccattta
    17221 tgaagaattg gatttcttcc cttgtcagct cttctccgtt tctttttttg gttatgatat
    17281 catacatcct cattacagcc gcacctcttt gataaattgt ttaaccaggg aaacaaacct
    17341 tggtttaacc gactcggcaa cctttactat ttcctcatgg gaaggcgctg tcattgtgtc
    17401 gggaatagcc atatccgtaa tgcaggacac tcccgcaacc ttgagcccgc agtgtcttgc
    17461 ggcaattgtc tccggcactg tggacatacc caccgtgtcg gctcccagct ttatcatcat
    17521 cgaaagctcc gcctttgtac aatagtttgg tccgcttacc gctccataaa cacccttgtg
    17581 cactcggatg ccctgggacg cggcaatctt ctccagcagt tcaaccaatt ccttgtcgta
    17641 cacctgtgac atatcgggaa accggggccc gaattcttca aggttgggcc ctatcagagg
    17701 gtttgttccc atgaaattta tatgatccgt aattatcata atatctccgg ctgcaaagtt
    17761 tggattcaat cctccgcagg cattgctggc aaccaggagc tttatgccca gctgcttcat
    17821 tacccttacc ggtatcgtaa cctcctgcat agtatagccc tcgtagtaat ggaatctccc
    17881 ctgcatggca attacctttt taccttcaag gcggcccagt ataagccttc cttcgtgccc
    17941 aaacactgta gatacgggaa attccggaat acccttatag tcaattaccg cttccttgtc
    18001 tatgtagtct gccaacgaac ccaggcctgt accgagtact atgcctgtct caggctcaaa
    18061 ccctgtattc tcccttataa aatccgttga tttttctatt ctttccctta attccatttt
    18121 cattaccccc aaattttatt ctcatattct ttccatagca cccctgataa gctttgaaaa
    18181 gctttcctta accctgtcgg ccgtttccat gacctccttg tggtttaacg gggtatccag
    18241 tatacccgct gccatatttg taatgcagga tattcccgca gttttaattc cgcagtgatt
    18301 tgccgccaat acttcaggaa cggtagacat tccaaccgca tcagccccca gtattctgaa
    18361 ggctcttatt tcagcaggtg tctcgtaact gggccctgta aggtataagt atgttccttc
    18421 ccgcagctta ataccctctg aagctgcgca ttccctgata atatccgcca gctcctttga
    18481 atatgccgcc gacatatcgg ggaaccgggg cccgaaggct tcaatatttg ctccgatcag
    18541 tggattatta ttggtataat ttatgtgatc tttaattatc atcaggtctc cgggtttgaa
    18601 gcttgtattt atgccgccgg ctgcatttgt cactataagc ttcttaactc ccagggaatt
    18661 gaaaacccat attgggaaaa caacttcctt catactgtag ccttcgtaat agtgaaatct
    18721 cccctgcatg aaaactaccc tcttgcctcc catagtgccg aatacaagcc ttcctttatg
    18781 gccctgcact gtggataccg gaaaacccgg tatttcctta tatcccacta tccttttatt
    18841 ctcagcttcc tccgccaggt cacccaggcc ggagccgagt atcagggcag cctcgggggc
    18901 agcccctgct ctttcagtta aataatcccc ggcttcattt ataagagaca ataagttctc
    18961 cttcatacac aatcaccccg tttgttaaat tttataaatt tcagttttaa aggatttacc
    19021 gtctatttct atgggcagcc ccaacaaatc cagcaccgtt gcacctatat cggaaaaacc
    19081 ttcccttgtg cctatatctg tcccttgcct tacatgcctg ccgtatacca gtatcggtat
    19141 gtattctctt gaatggtccg tgctgtccgt ggtcgggtca cagccgtgat ctgcagtaat
    19201 aatcaatacg tcggaatcct tcattgccgc aaaaatctct ggaagccttt catcaaaggc
    19261 cataagtgcc ccggcatagc cttccgcatc atttctgtgc ccgtaaagca tgtcaaaatc
    19321 aacaaggttc gaaaatataa gccccgtttt atcctctgac atgtattcca gcgtcttatc
    19381 cacaacatcc gtattgtcct ttgtgtgaac ggcctcactg ataccctccc ccgcaaaaat
    19441 atcctcaatt tttccgacag ctgctacatt cataccattt tcaactataa tatcaagcat
    19501 tgtcttttta attggcttca gggaaaaatc tcttctgttg gaggtgcgtt tatagcttcc
    19561 gctttttcca aggaagggcc ttgcaataac acgtcccacc gcatactcgt ccttcagcaa
    19621 ggctcttgct ttttcacata tttcatacag cttctgaaag ggtattacct cctcatgggc
    19681 tgctatctga aaaacactgt ctgcagaagt atatattatc ggatagcctg tttccacatg
    19741 cttatcgcca agccttgcaa ttatctccgt tcccgaagct gcttcatttc cgagcgtttt
    19801 tacacctata gccctttcaa aggcctccac aatctccttg gggaatcctt cgggaaaagt
    19861 gggaaagggc cgggacaaag taactcctgc tatttcccaa tggcctgtag tcgtatcctt
    19921 tcctggggag acctcttttg ccctgccgaa gcttcctgtc ggattctctg caatttgcag
    19981 gcctatgctc ctgtcaatgt ttcctagtcc aagagcttca agattcttca atttgaaatc
    20041 tttcacattc ctcaccaggc atcccagggt attgctcccg gaatccccgt acatatctgc
    20101 gtcaggcagc tcccctatcc cgacactgtc caacactatt agaataactc tgctaaccat
    20161 atttttacct cctaatttgg ataaccagaa tcctgtgtcc ggcgtctttt actgtctaaa
    20221 tattaaaaag ggctttttaa gcccttggat gagtcttgct gtatacttct tttatcctgc
    20281 tcttatttat cttgctgtac acttgagttg tagaaatatc cgagtgtcca agcatctcct
    20341 gtattgcttg aaggtcggcg ccgttttcta taagatgtac tgcaaatgaa tgcctaagag
    20401 tatgaggtgt tattgacttt tttatatttg cctttgccgt ataatatttt atgattttcc
    20461 aaaaaccctg tcgggtcatt ttctgcccgt ggaaattcac aaacaacagc ttgtggtttt
    20521 cattggtgca gagcttcttt ctgaaatcat tcagataagt ttttatatat ttaacggcaa
    20581 cattgcctaa aggtatggat ctggaattta tttcgccgtt cctgcatatt attaatcctg
    20641 aattcaggtc tacatcatca atattcaatg aaataagttc cgaaactctg atgccggtag
    20701 cataaagcag ctccagcatg gctttgtccc ttgcaccctt tgcatcagca ggtacaggct
    20761 gctcaagcag aagctccact tccttcttgg taagcactga gggaagcttt ttttctacct
    20821 ttggtgactc aagatttgcg gaaggatcca tatccaaata tctctcattc tgcataaatt
    20881 gataaaaaca ccttatggaa gcaagattcc tggaaatcgt tgaagttgct ttgcctgcct
    20941 tttgcaatgc aatcaaatat gtaatgataa tggtttttgt aaccttctcc aatgaaagag
    21001 catgttctct aagataaact tcgaattgtc ttatatctct tttataggat tccagcgtat
    21061 ttgccgataa ttccttatta ttaatgagat aatccaaaaa caactcaata aagctcataa
    21121 gaatcatatc ccctctatta cagtatcttt taactattca acacagataa taaaaatcct
    21181 ccaataaatg aaaatttttc atgaagcttt cttaatattt ttaatatttt ataaatggga
    21241 caattgcctt aactatatgg gatgttatgt acgcttccac aatactgcct gcgacgagca
    21301 gtatgaacag taccagcatc gtaagggcat aagcggcaaa caggcggggg gcagctgccg
    21361 atttcttaaa acacttgtac ctgagagccc acagagaata attaaatcct attacaccca
    21421 tgacgataat cacaggcaca aatataagat tctgaggcaa tacagaaccc aatgacagga
    21481 gaaatccatt accttctaca ttctcggtga gaaaag
//
LOCUS       DAOQUR010000038        21450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_2873_length_21450_cov_10.909278, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000038 DAOQUR010000000
VERSION     DAOQUR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21450
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_21450_cov_10.909278"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1422)
                     /locus_tag="PKI50_08615"
     CDS             complement(4..1422)
                     /locus_tag="PKI50_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT03801.1"
                     /translation="MLIHVVKPTETLWQIANRYRVSTASIAEVNGLPDPNRLVVGQAL
                     VIPTEDIFHTVLPGETLWKIAQAYGTTVQAILQNNQIANPDNIYPGLLLYIPALRYRI
                     QPGDTLWSVAQRFGVPLNSLIKINNIENVNLVYPGTVLVIPRKQRPPIDVNGYIYILG
                     QEAVPIIEEAGEHLTYLSPFAYLIREDGSLQAINDIPAIQAAKSYDAIPMMSITNFTS
                     TALGENLAHVVLNSPAVIERLLANIINIMKGKGYSGLNVDFENVLPADREPYNRFLQQ
                     AVTRLHQEGFFVSTAVAPKTSGVQTGLLYEAHDYEAHGRIVDFVILMTYEWGWRKGPP
                     QAISPLNQIRRVLDYAVTVIPREKIFMGFQIYARDWLLPFAWGQEAETFSVQEAVSRA
                     VRYGASIQYDTAAQSPFFRYTDAQGRAHEVWFEDARSTQAKFDTAKNYNLRGISYWAL
                     GYPFPQNWVLLEDNFTIRKGGS"
     gene            1705..1779
                     /locus_tag="PKI50_08620"
     tRNA            1705..1779
                     /locus_tag="PKI50_08620"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1738..1740,aa:Glu,seq:ctc)
     gene            1898..2344
                     /locus_tag="PKI50_08625"
     CDS             1898..2344
                     /locus_tag="PKI50_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT03802.1"
                     /translation="MKISTKGRYGLRAMVDLSVYSAGDHISLKSIAERQNISEGYLEQ
                     VFSTLRKAGLINSIKGAQGGYALADKPANITVGAVLRALEGNLSVIDEKSMEEKTNDK
                     IEECIREKVWSAINSSIGRVIDSITLEDLTNEYRKMTSNEAYMYYI"
     gene            2407..3342
                     /gene="cysK"
                     /locus_tag="PKI50_08630"
     CDS             2407..3342
                     /gene="cysK"
                     /locus_tag="PKI50_08630"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNT03803.1"
                     /translation="MKKVSKSITELIGNTPLLETVNYNSQNGIKGKLIAKLEYFNPGG
                     SVKDRVGYAMIKDAEDRGLVSKDTVIIEPTSGNTGIALALVAAARGYRLILTMPETMS
                     IERRSLLAALGAELVLTPGSDGMKGAIKKAEELVSEIPNAFMPQQFNNPANSAAHRNT
                     TAEEIWRDTEGQADIFVAGVGTGGTVTGVGEVLKGKNPDIRIIAVEPYDSPVLSGGTP
                     GPHKLQGIGAGFIPNVLNASIIDEIIKVKSEEAFAAARKLAAAEGILVGISSGAALHA
                     ATEVAKRPDSAGKNIVVLLPDTGERYISTALFQNA"
     gene            3484..3942
                     /locus_tag="PKI50_08635"
     CDS             3484..3942
                     /locus_tag="PKI50_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03804.1"
                     /translation="MGSNIRYISSRQQLIDEVETLLNNSQKLDIMVSGVWTKSLYEDI
                     IGLIMDKEFSSTCRVIIPKLIFKSSISLTLLRNLCKAEGQVRVNNSMTNNVLMIGRYA
                     FVLSFSSRLNSFNMLSTTFECSVMTDEVEIVNELKEQFDNIFENSVIFRM"
     gene            3980..5323
                     /gene="trkA"
                     /locus_tag="PKI50_08640"
     CDS             3980..5323
                     /gene="trkA"
                     /locus_tag="PKI50_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNT03805.1"
                     /translation="MKIIIIGAGKVGYNLAENLSKSNHDVIIIDKNYAALSKAEENLE
                     VLCIKGSGVSTNILMEAGIETADLLIAVTNSDEVNMVCCLTAKKLGVKHTIARIRDHE
                     YAHELLLLKEELGLDLIINPEQAAADEIANVLNFSSAINVENFAKGRVKMIEIKVAED
                     MPMVGMRLKDIGSKFESSVLVGVVIRDDEVIVPNGDFIINENDSIYLIGQPSHLYNFC
                     KVFGKVPHKIRNVMIVGGGRIAVYLGKLLAEMGMKVKVIEIDREKCIQLSELIPEALI
                     IHGDGTDEELLRSENIDEMDAFIAVTGMDEENMMAALIAKHNGAKKVISKASRTNYIS
                     IIKSLGIDSVISPKQITTNQILKYVRGNSVESLYRIIEGQAEITEIIAEPESKILNTK
                     IKKLGLPKDTIIATIVRKNEVVIPHGNDIIKEGDRVIVISKNKNISSLDEVAAVS"
     gene            5337..6788
                     /locus_tag="PKI50_08645"
     CDS             5337..6788
                     /locus_tag="PKI50_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNT03806.1"
                     /translation="MNFRLVLRVLGMVLACEALFMLPSLAVSVYFGGDDIAAFALSIL
                     ITAVIGIPLTYIKANKHGMYARDGFAIVALGWILLSFFGSLPFVISGAIPSYIDAFFE
                     ASSGFTTTGASILTQIERLPKGILFWRSFTHWVGGMGVIVLTLAILPSVGAGGLQMMK
                     AESPGPTPGKIVPKVAETAKILYGIYIVITIVEIIALKMAGLPLFDSAIHTFGTVGTG
                     GFSNMNTSVGEYNNVYAEAIIAVFMFICGVNFSLYYQMLKGDLKAAFKDEEFLFYLRT
                     VLIAIILITINLYGNVFSSVWESLRHSSFQVVSIISTTGYVTSNFEEWGMFSKIILFM
                     LMFVGGCAGSTGGAIKNIRFLLLFKVMKRELLQIIHPKAVYSVRLGGRAVNERTISEV
                     LGFFFMYIIIFIAAVLIISLDNLDWATTIGSVAATIGNIGPGFGIVGAVGNYSTLSNL
                     SKIVLSMCMIIGRLEIYPILLLGMPSFWKRTSV"
     gene            complement(6785..7474)
                     /locus_tag="PKI50_08650"
     CDS             complement(6785..7474)
                     /locus_tag="PKI50_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT03807.1"
                     /translation="MCGCEHRRNHANCIESVPIFGSLSREEMLEIANITSAKTFEKGD
                     MVYMAGDKSNNLYVLHTGRVKISRLGKNGKEQVIRVIGPGEFMGELSLFGSLPLTDNA
                     EVLETSTMCVIEGSKLKELMAKYTSIAFKVMGELSKRLEKAENLIEAISLNSVEQRLA
                     QALLALADRKNEVTLNMSKGDFASQMGMSQETLSRKLAVFQAEGIIELKGHKKIIILN
                     KAGLEEISAAV"
     gene            complement(7608..8300)
                     /locus_tag="PKI50_08655"
     CDS             complement(7608..8300)
                     /locus_tag="PKI50_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HNT03808.1"
                     /translation="MKKNLKSIICVTIICLLLVGQTIGVFADTYYGLLKFGSRGTAVV
                     KLQQALINKGFSPGAADGIYGKMTENAVIKFQQANKLRIDGIAGRETQSRLFAAAAVA
                     SRGTSYTSTDLYWLSRIINAEAEAEPYAGKVAVGNVILNRVKSGSFPNKVKGVIFEYY
                     KGIPQFSPVADGTIYNDPNADSIKAAKAAFSGTSYAGNATYFFNPNKSEGTWIVNNKT
                     FVKRIGNHVFYK"
     regulatory      complement(8474..8607)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(9084..10919)
                     /locus_tag="PKI50_08660"
     CDS             complement(9084..10919)
                     /locus_tag="PKI50_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT03809.1"
                     /translation="MPDLKRIVILGAGYAGVEAAKVLGKKFKKDDSVEITLINDNPFQ
                     TLLTELHEIAGRRTEKESVMVDLYKVFKATKVNFVRDRIESIDYEAKTLTSSKGTYSY
                     DYLVLGCGAEPAYFGIEGVKEYGYTIWSLKDALKIREQVLKSFEAARDEKDAAKRKEL
                     LTFVVAGAGFTGIETVGELFEWKDVLCDEYGIDSKEVTIYNVEAMPTILPILRPSLQA
                     KAASFLTKKGVKLMTNSPIVKATPDGIVLKDGQEIKTKTLIWTCGVQGNSFTASSGLT
                     PGKRNRVQVNDYMQSMDKEEVYLVGDNAYYEIDGKPIPQIVETAIQTGECAAHNIAAD
                     IAKTEKKKFKLNTHGFMVSIGSHYCVAELMGTPLSGFIAMAMKHLVNLHYLWGVGGVR
                     LIWNYLLHEFFHMKNNRAFVGGHLAHRTHTSWLVLLRLYVGVMWFLEGWKKVQEGWLN
                     PANIKIVSVAGTSGSSAAGAAAEAVTSASQAAGAAGEAAAAAGPVPILAEPLGIYQWF
                     QDTFIIANPFFFQAVVVLMEVGIGLALMAGLFTFFASLGSLFLTANFIVSAMAGYDIL
                     WYVFASIALMGGAGGAFGLDYYVQPWIKKAWKKTKFAQASYLYFD"
     gene            11336..12709
                     /gene="amrA"
                     /locus_tag="PKI50_08665"
     CDS             11336..12709
                     /gene="amrA"
                     /locus_tag="PKI50_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNT03810.1"
                     /translation="MGKIAGAFIFPHPPIMVEEVGGSETKKVSNTVVGALEASERIAE
                     IKPDVIVLITPHGAMLKDAMVIAADERLEGSLARFGAPGTKLSFDNDLELADKIMEQA
                     DKKQAYCIPMHEDVKNTYRYASGIDHGAMVPLYFVSKKYRHFKLVHINYSLLSREQHY
                     KFGIAIRDAVAELDRRAVIIASGDLSHRLTKNAPAGFDPRGEEYDKKYMKIIKEGDVT
                     GLLSMPAGLLEDAGECAYNSTVVLMGCLDGCSVKGEVFSYEGPFGVGYCVAEIHAEAC
                     ADKKKVISSDPYVQLAIDTLETYIKTRRKIDVPEGLPDEMTTRRAGVFVSLKKYGELR
                     GCIGTIKPTTKNIAAEIIQNAISAGTRDPRFLPVEESELSELAYSVDVLGEPEPINTK
                     DELDVKRYGVIVRSGYKSGLLLPNLEGVDTVDYQVSIALQKAGIRPGEPYKMERFEVV
                     RHYQYME"
     gene            12725..13729
                     /gene="amrS"
                     /locus_tag="PKI50_08670"
     CDS             12725..13729
                     /gene="amrS"
                     /locus_tag="PKI50_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT03811.1"
                     /translation="MKEAKHYEKQEGGNVKCRLCPHECNIAPGKYGICRVRNNIGGIL
                     YTHNYGKISSIAMDPMEKKPLYHFFPGRYILSIGTVGCNLRCSFCQNYEIAQRGAAEE
                     GGEDNLYEASEAYLLSLCRKEEDCIGVAYTYNEPSIWFEYILDTAGHIRSEGFKNVLV
                     TNGYISREALSELLPYIDAMNIDVKGFTEKYYRNICGGSLEHVKKTVEAAAAECHVEI
                     TTLVVPGHNDSEREIQELSKWLSSINPSIPLHLSRYFPRYRMKEQPTPAGTLISLKKA
                     AESYLEYVYIGNMQSEAVNTYCPQCGALLIRRTGGIIIEHLDDGICFKCGKAISITGI
                     "
     gene            13827..14645
                     /locus_tag="PKI50_08675"
     CDS             13827..14645
                     /locus_tag="PKI50_08675"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HNT03812.1"
                     /translation="MNFFVQMRESVIDFKFYRSIRDNKFSRSFVYLLLLFLIIYFING
                     TRTFIVTRIFMDDLAVHLAENVPEFRLENGVFSFEGDMPYYISSSTNEAFIIDTTGQV
                     TESILEDAGTGMLITRDKIFVKRNKIETRQFSLSELEGFTLAKSDVLEFMPKLSWIVF
                     ILIAFSFIFVLGWKLLNAVILALLGIAVNALLKGRLRYNNLLNISIYALTLPMLVQLA
                     VNLYGYPIPGFGLIYWLISILYLDLAIKSCIDQDKESEAGNELEDIKGEDRSNF"
     gene            14623..15108
                     /locus_tag="PKI50_08680"
     CDS             14623..15108
                     /locus_tag="PKI50_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT03813.1"
                     /translation="MKIGVISDTHIRSSAKLLPNIIYSVFDGVDAIFHAGDILIDEVI
                     IELETIAPVYAVAGNNDSYEMLYKYSTKRIIEANGKRIGLTHGTSRVNAYAGAYSMFA
                     KNNVDCVVYGHSHKPHNEVIDGVLFFNPGSPTSKRFESRYSLGLLHIGRDIRGEIIYF
                     D"
     gene            15374..15907
                     /locus_tag="PKI50_08685"
     CDS             15374..15907
                     /locus_tag="PKI50_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNT03814.1"
                     /translation="MLEKILEHNRKFVQERKAAGEDKPISGHAHKDIMVFTCMDTRLV
                     ELLEPAMGFGRGDIKILKNAGNIIRNNCSDVIRSVAVGTVLMGLKEVYVVGHSDCGMR
                     KQKPEDIRAKMRERGIPEEAIEAIDIKEWFGLLQDEAENVKDAVLKLKNSPYIPKDVK
                     IHGLLMDPNSGEISVIE"
     gene            16053..16865
                     /locus_tag="PKI50_08690"
     CDS             16053..16865
                     /locus_tag="PKI50_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNT03815.1"
                     /translation="MEKRMLTPKEIAMATCDAGAAKAKLSIAQMMVLGIFAGVFIGFG
                     AQGAITIGQTLAKVDTGLQKFAFAGAFPVGLMLVVICGAELFTGNNLMTLGCLNCRYK
                     VSDILRNWFFVYTANFIGSVILAAIIANTGLMAAGTPIAESAIKIASNKVAIPFGAAV
                     LRGILCNIMVVLAVWMATGAKDVISKIFACWFPIMLFVLCGYEHSVANMYFIPLGMFL
                     GAPVTWGQIWINNLIPVTIGNLIGGALIVPLFYHVAYMKAPTAAAPTKDAKA"
     regulatory      16906..17037
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            17150..18130
                     /gene="moaA"
                     /locus_tag="PKI50_08695"
     CDS             17150..18130
                     /gene="moaA"
                     /locus_tag="PKI50_08695"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNT03816.1"
                     /translation="MKDELGRNINYLRISVTDRCNLRCRYCMPEEGIEKKEHLDILTL
                     EEIFEVVRVCADLGTNKIRITGGEPLVRNGLTGLIERITALESIKDLALTTNGSLLKK
                     YARELKKAGLKRINISLDTMDDKKYEYLTRGGKLKDVLEGFHEALEVGLKPIKINTVL
                     TKGFNEDEIGDFIRLTQNYDIDVRFIELMPLGHAAGFASKHYISNLVVLERYKELELA
                     EAADKSSPAMYYKLPGAKGRVGFINPISHKFCDNCNRLRLTADGKLKPCLHSNLEIDV
                     KGILRGRNSDDRYLALQKAVEEAVQAKPGHHTLNDLYNKPIDRDMYTIGG"
     gene            18148..18591
                     /locus_tag="PKI50_08700"
     CDS             18148..18591
                     /locus_tag="PKI50_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNT03817.1"
                     /translation="MKGKVLAVNISEKKGVFKKPIEQGEFKVDHGLAGDAHAGNWHRQ
                     VSLLGIESINKMKALGIEGLSTGKFAENLTTAGLELWKLPVGTKLKIGETEQEVTQIG
                     KECHVKCEIFQKIGSCIMPTEGIFTKVLKGGIIKPGDEIEVQGDV"
     gene            18690..19001
                     /locus_tag="PKI50_08705"
     CDS             18690..19001
                     /locus_tag="PKI50_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT03818.1"
                     /translation="MPDFGNSFSGLAKDRKLTDQELIRAIRFMVAAEYEAIQLYMQLA
                     ESTDNMLAIEVLKDIADEERVHAGEFLRLLYELAPDEEKFYAEGAEEVEEEIEAFKRK
                     K"
     gene            19285..20340
                     /locus_tag="PKI50_08710"
     CDS             19285..20340
                     /locus_tag="PKI50_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNT03819.1"
                     /translation="MAICVRKGKPGVLELVFPYNEEYIAKVRGIPGRRWDKDNKKWLV
                     PDCEESINQLQELFTKEELVIVKGSSNDQFLKKMEEGLKLRGYSQKTIKSYMSHARLF
                     LGYCGKNANQLVKEDINCYLLYLLEQKGNAHSFVNQAVSSIKFLFGKVLMEDDLTLDI
                     VRPKKEKKLPEVLSQNEVRHILDNVTNFKHKAILFLIYSSGLRVGEVVRLKLKDIDSD
                     RMLIHVVQGKGRKDRYTVIAEVALGVLREYVQKFRPVEWLFQGEEVGRHITERTVQRV
                     FENAKEKSGIQKKVSVHSLRHSFATHLLEGGIDLRYIQELLGHQSSKTTEIYTHVTEK
                     GIKNIASPLDKLMSRRE"
     gene            20496..20882
                     /locus_tag="PKI50_08715"
     CDS             20496..20882
                     /locus_tag="PKI50_08715"
                     /inference="COORDINATES: protein motif:HMM:NF022172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HNT03820.1"
                     /translation="MKLNHDCIRALLIYLEENLEVKANGLPEGLKLGHIDEDDLSGFS
                     QEDIFYSAKKLVEAKYIEVANKEVAPRAMIIKEITWNGHDYLDSIRDPKVWYEIKSRT
                     KDLSGIALEVVKSLAIEITKQMLGLG"
BASE COUNT         6540 a         4039 c         5001 g         5870 t
ORIGIN      
        1 tgcttacgac ccgccttttc tgattgtaaa gttatcctcc aacaataccc agttttgcgg
       61 gaagggatac ccaagagccc aataactgat cccccgcagg ttatagtttt ttgccgtatc
      121 aaattttgcc tgggtgctgc gggcatcctc aaaccatact tcgtgtgccc tcccttgcgc
      181 atccgtatac ctgaagaagg gcgattgggc tgctgtatca tactgtattg aggcgccgta
      241 tctgacagcc cttgatacag cctcctggac actgaaggtt tctgcttcct gcccccaggc
      301 gaaaggaagc agccagtctc ttgcataaat ctgaaagccc ataaaaatct tttctcttgg
      361 aatgactgtg actgcatagt caagaacacg ccgtatctga ttcaaaggcg aaattgcctg
      421 cggagggcct ttacgccagc cccattcata cgtcatcaga attacaaaat caactattct
      481 gccgtgggct tcataatcat gggcttcata cagcaagccc gtctgcacgc cgcttgtttt
      541 tggagctact gcagtagata cgaaaaagcc ctcctggtgc aaacgggtta ccgcttgctg
      601 aaggaagcgg ttataaggct cacggtcagc cggaagcacg ttttcaaagt ccacattcaa
      661 tcccgagtac ccttttcctt tcatgatatt aatgatattg gcaagcagcc gttctatgac
      721 tgccggactg tttaatacga catgggcaag gttttccccc aaagcagttg aagtaaaatt
      781 tgtaatggac atcatgggta tggcgtcata agatttggca gcttgaattg cagggatgtc
      841 attaatggcc tgcaggcttc cgtcctctct tatcaagtaa gcgaaggggc ttaggtatgt
      901 cagatgctca cctgcctcct caattatcgg aacagcctcc tggcccaaaa tgtagatata
      961 cccgttcaca tcgattggag gcctctgctt ccgggggatt accaatacag taccgggata
     1021 aacaagattc acattttcaa tattatttat ctttatcaga ctgtttaaag gaaccccaaa
     1081 cctctgggcc acagaccata atgtatcacc gggctggata cggtatcgga gagcgggaat
     1141 atataatagc aggccgggat atatattgtc gggattggca atctggttat tctgaagaat
     1201 tgcctgaaca gtggttccat atgcttgggc gattttccac aatgtttctc ccggtaatac
     1261 agtatgaaaa atatcctccg taggtatgac caaagcctga ccgactacca gccgattcgg
     1321 atccggaagc ccgtttactt ccgctatcga tgcagttgag acacgatagc ggtttgcaat
     1381 ttgccatagt gtttctgtcg gttttacaac atgaatgagc ataatgcacc tcctttcatt
     1441 cactaacaat atatgaagaa atattttcat tatgatagcg gaatgtacct atggcttcta
     1501 cctattctat gggaaccaaa gacacattca caatggacgc aggcagctcc gtatccggag
     1561 tgctggaagt gaaggcaaaa tataggtgat tattattgga ttgcacggtt ttattaatag
     1621 aataatacct ggtcttatta taattatttt cgatttacaa aagtatgtgt atcaattata
     1681 atagtaccgt agtaattttt ataaggccca ttggtcaagt ggtcaagaca tcgccctctc
     1741 acggcggtaa caggggttcg aatcccctat gggtcaccaa taataaaata atctttaatt
     1801 aaaaatgcaa ctaagttgca ttttttcatt gtaattctta aaaagcgatg ctataatagt
     1861 aatattataa aacatattgg aatagtagga gatgcacatg aagatttcta caaaaggcag
     1921 atatgggcta agggcaatgg tggatctttc cgtatactct gcaggagatc atatatcatt
     1981 gaaaagcata gccgaaagac agaatatatc agagggctat ctggaacagg tcttctcaac
     2041 cctgagaaaa gcaggcctga ttaatagtat aaagggggca cagggggggt atgctttggc
     2101 ggataaacct gcaaacataa ctgtaggtgc tgtattgagg gctttggaag gaaatctttc
     2161 agttattgac gaaaaaagca tggaagaaaa aactaatgac aagattgagg aatgtataag
     2221 ggaaaaggta tggagtgcaa ttaactcaag tattggcaga gtgatagaca gcattacact
     2281 ggaggatttg acaaacgagt atagaaagat gacaagcaat gaagcatata tgtattacat
     2341 ataatatttt aacgcaaaac ctaatattca aataggacaa atagtataaa atggaggaat
     2401 attgtcatga aaaaagtatc aaaaagcatc actgaactca tcggtaatac accgcttctg
     2461 gaaaccgtca actataacag ccaaaacggc ataaaaggaa agcttatagc aaagctggaa
     2521 tatttcaatc cgggaggcag cgtaaaggac agggtaggat atgcaatgat aaaggatgca
     2581 gaagatagag ggcttgttag caaggatacg gtaataatag agcccacaag cggcaatacc
     2641 ggtatagctc tggcacttgt tgcggcagca aggggttaca ggcttatact tacaatgcct
     2701 gaaacaatga gcatagaaag aagaagcctt cttgcagcat tgggagcaga actggttctt
     2761 acccccggca gtgatgggat gaagggtgcc ataaaaaaag ctgaggagct ggtttctgag
     2821 attccgaatg cttttatgcc ccagcagttt aacaatcccg cgaactcagc agctcacagg
     2881 aatacaacag cagaagaaat ctggagggat accgaggggc aggcagatat atttgttgcc
     2941 ggagtcggta ccggcggaac tgttacagga gtcggagagg tgctgaaagg aaagaatccc
     3001 gatatcagga taattgctgt ggaaccttat gattcaccgg tattgtcggg gggaaccccg
     3061 ggtccccaca agctgcaggg tataggcgca ggttttattc cgaatgtatt gaacgcttca
     3121 attatagatg agattattaa ggtaaaaagc gaggaagcct ttgcggcggc cagaaagctt
     3181 gcggctgcgg agggtatatt ggtcggtatt tcctcggggg cagcgctcca tgcggcaacg
     3241 gaagtcgcaa agaggcctga cagtgccggc aaaaatattg tagtactgct tccggatacg
     3301 ggagaaaggt atatatccac agctctattt caaaacgcat agcttctgta agaaaacccc
     3361 tgccaaatca aaaataggca ggggttttct tctaatgata agcttgctta tagctgtcat
     3421 tatctgtaga atgatattat agttttatat ttatttttaa tgagaaaagt ttggaggtgt
     3481 gccatgggga gcaatattcg atatatttca tccagacagc agcttattga cgaagtagaa
     3541 accctattga acaatagtca aaaacttgat attatggttt ctggagtatg gacaaagtca
     3601 ctatatgaag atataatcgg attaattatg gataaagaat tcagcagtac ttgcagagtg
     3661 attataccaa agctgatttt caagagcagc atttccctga ctttgctaag aaacttgtgc
     3721 aaggctgaag gacaggtgcg ggtaaacaat tctatgacca acaatgtgct gatgataggc
     3781 agatatgcat ttgtactttc tttctccagc agactgaaca gttttaatat gttaagtact
     3841 acttttgagt gctcagtaat gactgacgag gttgagatag tcaatgagct taaggaacaa
     3901 tttgacaaca tctttgaaaa tagtgtaatt tttaggatgt aacgctgcaa gtaaaaacat
     3961 ttatatgggg gcttaaggaa tgaaaattat cattatcggc gccggaaaag tagggtataa
     4021 tctggcggaa aacttatcaa agagtaatca tgatgtaatt ataatagaca agaattatgc
     4081 tgctttgagc aaagcggaag agaaccttga agtgctttgc atcaaaggaa gcggagtaag
     4141 taccaatatt ctcatggaag cgggaataga gactgctgat ttgctaattg ccgtgacaaa
     4201 cagtgatgaa gtaaatatgg tctgctgtct caccgccaag aaattgggag ttaaacatac
     4261 aatagcaaga ataagagacc atgaatatgc acatgagctt ttgctcctca aagaagagct
     4321 tgggcttgac cttatcataa atcccgagca ggctgctgcg gatgaaattg caaatgtgct
     4381 gaacttctct tctgcaatca atgttgaaaa ctttgcaaag ggcagggtta aaatgattga
     4441 aataaaagtt gcggaagata tgcccatggt gggcatgcgc cttaaggaca tcggaagtaa
     4501 gtttgaatcc tctgttttgg ttggagttgt aatacgggat gatgaagtta tcgtacccaa
     4561 cggggatttc ataattaatg agaatgacag catttatctt atagggcagc cttcacacct
     4621 gtacaatttt tgcaaggtgt tcggcaaagt tcctcataag attagaaacg taatgattgt
     4681 aggcggaggg agaattgctg tttaccttgg aaagcttttg gcagagatgg gcatgaaggt
     4741 caaagtaatt gagattgaca gagaaaagtg tattcagtta tctgagttga ttcccgaagc
     4801 acttatcatc catggggatg gaacagatga agaactgctg agatctgaga atattgatga
     4861 gatggatgcg tttattgcag tgaccggaat ggatgaggaa aacatgatgg ctgcactgat
     4921 agcaaaacat aatggtgcca aaaaggtcat atccaaggca agcagaacca actatataag
     4981 cattattaaa agcctgggta tagacagtgt aataagccca aagcagatta ccaccaatca
     5041 gatattaaaa tatgttcgcg gcaacagtgt tgagtcatta tacagaatca ttgagggaca
     5101 agcggagata actgaaatca ttgcagaacc ggaaagtaag atattaaata cgaagatcaa
     5161 gaaactggga ctcccaaagg atactattat tgctaccatc gtcaggaaaa atgaagttgt
     5221 aatccctcat ggaaatgata ttatcaaaga aggggataga gtaattgtga tttcaaaaaa
     5281 caagaatatc tccagcttgg atgaggtagc tgcggtttct taaggaggat atgatattga
     5341 actttagatt agtattaaga gtcttgggca tggttttggc atgtgaagca ttgttcatgc
     5401 tgccctcact ggcggtatcg gtatattttg gaggagatga tattgctgca tttgctttaa
     5461 gtatattaat cactgcggtt attggcatac cgttaaccta tatcaaagca aacaagcatg
     5521 gtatgtatgc ccgtgacggt tttgccattg ttgcattagg atggatacta ctgtccttct
     5581 tcggttcgct tccttttgtg atcagcggtg ccataccttc atacattgac gcatttttcg
     5641 aggcaagctc cggatttacg actaccggtg caagcatact tacacaaata gaaaggcttc
     5701 caaagggaat acttttttgg agaagcttta ctcactgggt aggcggcatg ggcgttatag
     5761 tactcacctt agctattctg ccttcagtag gtgccggagg attgcaaatg atgaaagcgg
     5821 aaagccccgg gccgactccg gggaaaattg tacccaaggt ggctgaaaca gctaaaatct
     5881 tgtatggtat atatattgtg ataactattg tggaaatcat tgcgctgaaa atggcgggat
     5941 tgcctctttt tgattctgcc atacatacct ttggtacggt cggaacgggc ggattttcca
     6001 atatgaacac aagcgtcgga gaatataaca atgtgtatgc tgaggctatt atagcagtat
     6061 tcatgttcat atgcggtgtc aacttctctt tatattatca gatgctgaag ggcgacctta
     6121 aagctgcttt taaggatgaa gaattccttt tctatttacg aacagtgcta atagctataa
     6181 tcttaataac aatcaacctg tatggaaatg tattcagctc cgtctgggaa tcgctgaggc
     6241 attcatcctt tcaggttgta tcaataatca gtactaccgg atatgtaaca tcgaattttg
     6301 aagaatgggg aatgttcagt aaaattatac tgtttatgct gatgtttgtc gggggatgtg
     6361 caggttccac cgggggcgca ataaaaaata tcagatttct gctattgttc aaggttatga
     6421 aaagagagct gctgcaaata atacatccaa aagcggttta ttcagtcagg cttggcggca
     6481 gagcagtgaa tgaaagaaca atttccgagg tattgggatt ctttttcatg tatattataa
     6541 tttttattgc ggctgttctt attatttccc ttgataatct tgattgggca acaacaatag
     6601 gatctgttgc tgctactatt ggaaacatag gacccggttt cggtattgta ggtgcggttg
     6661 gcaactactc gacactgtcg aatttaagca agatagtatt atcaatgtgt atgattatag
     6721 ggagattgga gatatatccc atcttgcttc tcggtatgcc ttccttctgg aagagaacaa
     6781 gtgtctaaac agctgcgctg atttcctcca gtccggcttt gttcaaaatg ataattttct
     6841 tatgcccttt cagctcaatt atgccttctg cctggaatac agccagtttt ctgctcaagg
     6901 tctcctggct cattcccatc tgagaggcaa aatctccttt gctcatattc aaagtaacct
     6961 catttttcct gtcagctaaa gctagtaaag cctgtgccaa acgctgttct acggaattca
     7021 ggctaatagc ttctatcaga ttttccgcct tttccaatcg tttactcagt tcacccatca
     7081 ccttaaaggc aatggaagta tactttgcca taagctcctt aagctttgat ccttcaatta
     7141 cgcacatagt tgaggtttct aatacctctg cattatcggt caagggcaag gacccgaaga
     7201 gtgagagttc gcccataaac tcacccggcc ctatcacacg gattacctgc tctttaccgt
     7261 ttttgcccag ccttgaaatc ttcactctgc cggtatgcag cacataaaga ttgttgcttt
     7321 tatcgccggc catatatacc atatcgcctt tttcaaaggt tttagcagag gtgatattgg
     7381 cgatctcaag catctcctca cggctcaggc ttccaaatat cggcacactc tctatacaat
     7441 ttgcgtggtt ccttctatgt tcacatccgc acacccaaaa cgcctcctat ataaacccgg
     7501 aatttttttt gccaatatct actattgata tgataccaca aatgctgtcc ttaggccaac
     7561 gaaaaacggc acagccgctt actgcggcta tgccgttttt tcaagtttta tttgtaaaac
     7621 acatggttac caattctttt aacaaaggtc ttgttattta cgatccacgt accttctgat
     7681 ttattcggat tgaagaaata agttgcattt cccgcatagc ttgtcccgct aaatgcggct
     7741 tttgcagctt ttatgctgtc cgcattcgga tcattataaa tagtaccgtc tgcaaccggg
     7801 ctgaactgag ggatgccttt ataatactcg aatattacac cctttacttt attggggaat
     7861 gaacctgact ttaccctgtt caaaataaca ttaccgacgg caaccttccc tgcatagggt
     7921 tcagcttccg cttcggcatt gattattctt gaaagccaat acagatctgt tgatgtatag
     7981 cttgtccccc ttgaagcgac ggcagcagcc gcgaaaagcc tgctttgagt ttccctgcct
     8041 gcaataccgt caatcctgag cttatttgcc tgctggaatt taatcaccgc gttttccgtc
     8101 attttgccgt aaataccatc ggctgctccc ggtgaaaagc ccttgtttat taatgcctgc
     8161 tgcaatttta ctacagcagt gcctctgctt ccgaatttca acaaaccgta ataggtatcc
     8221 gcaaaaacgc ctatggtttg tccgactaaa agcaaacaaa taatagtaac acaaataatg
     8281 cttttaagat tctttttcat acacaataca ctccttccat aattgagtcc cttgtaacac
     8341 ctaaaagctg catgatactt gtctattcgt tccactactg cgttgatgtc aatcgccgca
     8401 gccaccgcta cgcctctttc catcgccttg tactggaagc aaatatcctg cgtatcataa
     8461 cgcaacagtc aggtattaca aggtactagc ctccgaagtt agctgtcggg tttgggacag
     8521 aaggttccct accacggttg tccatggatt aaccccagaa ttggttcctc cgtacccggc
     8581 aaagccggga ttcagcaaat gtttgtgttt acataatgca tattatataa taaatcaagt
     8641 tatatgtcaa atcaacgcag aaattggaaa acatgaagta aacataaagt atttgaataa
     8701 agcttaagag ggaaatttgc cctcttaagc tttaatgttt tacaaagact acattgattg
     8761 ctttaaccag agcatctact ctgtcgccct gcttgaaacc cgcatcatca aggctttctc
     8821 tcgtcatcac ggacgtgaat ctcatggtat tgtcctgtca tgtgcctctc atcttgactt
     8881 ccgccatgat ttcgtcagct tttattgctt cgatttcacc tgacaccctg tttcttactc
     8941 ctgcttccac aagaaatcct cctttagatt gtttttggaa tactatcaga taatagtttc
     9001 tagatttaaa tgggatatta ttcatagaac gcaaataaaa aacaaacacc gaaaatccgg
     9061 tgtttgtttt ttattctaat attttaatcg aagtacaaat aacttgcctg ggcaaacttc
     9121 gtttttttcc aagctttttt aatccagggc tgtacataat agtcaaggcc gaatgctccg
     9181 cctgcgccgc ccataagagc gatggaggcg aatacgtacc agagtatgtc gtagcctgcc
     9241 attgcagata caataaagtt tgcagtcagg aataatgagc ccagtgatgc aaagaatgta
     9301 aagagtcctg ccataagtgc aaggcctatt ccaacttcca tcaatacaac aactgcctgg
     9361 aagaagaacg gattagcaat gataaatgta tcctggaacc actgatatat tcccagaggt
     9421 tctgccagta tcggcaccgg ccctgctgcc gctgcagctt ctccggcagc tcccgctgcc
     9481 tgtgaagcgc ttgttacggc ttccgctgcc gctcctgctg ctgatgagcc tgaagttccc
     9541 gcaaccgata ctatcttgat attagccggg ttcagccagc cttcctgtac ctttttccaa
     9601 ccttcaagga accacattac gccgacgtat aaacgcagaa gcaccagcca ggaagtatga
     9661 gtcctgtgtg ccaggtgtcc gcctacaaaa gccctgttgt tcttcatatg gaagaattcg
     9721 tgcagaaggt aattccatat gagccttacg ccgccgactc cccacaagta gtgaaggtta
     9781 acaaggtgct tcatagccat ggcaatgaag ccggataagg gtgtgcccat aagctccgct
     9841 acacagtagt ggcttccgat ggaaaccata aagccatgag tgttgagctt gaattttttc
     9901 ttttcagttt tggctatatc cgctgctatg ttgtgggctg cacattcacc tgtctgaata
     9961 gccgtttcaa ctatctgcgg tatcggtttt ccgtcaattt cataatatgc gttgtcaccg
    10021 acaagatata cttcttcttt gtccatgctc tgcatgtaat cattaacctg cactctgttt
    10081 ctcttgcccg gagtcaggcc tgatgaagcc gtgaaactgt tgccttgtac accgcaagtc
    10141 cagataagag tctttgtttt tatctcctgt ccgtccttaa gaacgatacc gtcaggtgtt
    10201 gctttgacta taggtgaatt tgtcattagc tttacgcctt tttttgtaag aaagcttgca
    10261 gctttagcct gaaggcttgg ccttaaaatc ggcaatatag taggcattgc ttcaacatta
    10321 tatattgtaa cttctttcga atcgattcca tattcatcac acaatacatc tttccattcg
    10381 aagagttcac ctacggtttc aattcccgtg aagcctgccc cggctacaac aaaggtaagc
    10441 agttcttttc tctttgctgc atctttttca tcccttgcag cttcaaagct ctttaagacc
    10501 tgctctctga ttttcagagc gtctttaagg gaccatatag tataaccata ttctttaaca
    10561 ccttcgattc cgaagtatgc aggttctgct ccgcagccaa gtacaaggta atcatagctg
    10621 tatgttcctt tgcttgatgt cagagttttt gcttcataat caatgctttc tattctgtcc
    10681 ctcacaaaat tgactttggt agccttaaat accttgtaaa ggtctaccat tacactttcc
    10741 ttttctgttc tacgtccggc aatttcatgc agctcagtaa gaagagtctg gaaaggatta
    10801 tcattaatta aagttatctc aacgctgtca tcctttttaa acttcttgcc cagaacctta
    10861 gctgcctcga ctccagcgta gcctgcgccg agtatgacta ttctttttaa atccggcaaa
    10921 atgattcctc cttgtatata attaactatt aaagaataaa aatattaagt ataataatat
    10981 tcattatcat tatataatat atatcaaaga aatgcgatag tttttatcgc tattttgtga
    11041 aaaaaatctc agttttatta atggtatatt atcactgccg aatatcaagc cgttcagcct
    11101 atcatacctg aagaatttta ctttcaattc cttattgctg ctgtgccgga aatcattact
    11161 ccaattattt caaaaattag caatgatgta aaaatttaaa tcgaattagt tattcaaaaa
    11221 ttatataatt gaaatagaaa aattgtgcgg caatttgtcc cggaaggatt aatattatca
    11281 gccggggaat attaaaaaaa tggaaatata tgaagtgtca gaaggaggaa agaagatggg
    11341 taagatagca ggtgctttca tttttccgca tcctcctatt atggtggagg aagttggggg
    11401 aagtgagaca aaaaaggttt caaacacagt tgtcggtgct ctggaagcct cagagcgcat
    11461 cgctgaaata aaaccggatg ttatagtgct tataacacct cacggtgcga tgctcaaaga
    11521 tgcaatggtt attgcggcgg atgaaaggct ggaggggagt ttagccaggt ttggagcccc
    11581 ggggacaaag ctgagctttg acaatgactt ggagctggcg gacaaaataa tggagcaggc
    11641 tgataaaaag caggcatatt gcattcccat gcatgaggat gtaaagaata catacagata
    11701 tgcgtcggga atagatcatg gcgccatggt ccccctttat tttgtgagta agaaatacag
    11761 gcatttcaag ctggtgcata taaactacag ccttctttca agggagcagc actataagtt
    11821 tggcattgcc ataagggatg ctgtggctga gcttgacaga agggcagtca taattgcaag
    11881 cggagatctt tcccacaggc ttacaaaaaa tgctcccgca ggttttgacc cccgaggaga
    11941 agaatatgat aaaaagtata tgaaaatcat taaagaagga gatgtcacag gactgctttc
    12001 catgcctgcc ggccttctgg aggatgcagg ggaatgtgcc tacaattcca ctgttgtgct
    12061 tatgggctgt ctggatggct gcagcgtgaa gggagaggta ttttcctacg aaggcccctt
    12121 cggggtaggc tactgcgtgg ctgagattca cgcagaggcg tgtgcggata aaaagaaggt
    12181 tataagcagc gatccatatg tgcagctggc tatagatact ctggagactt atataaaaac
    12241 aaggcgcaaa atagatgtgc ctgaaggttt gcctgatgaa atgaccacac gcagggcggg
    12301 agtttttgtt tcgctaaaga aatacggcga actcagaggt tgcataggga ccataaagcc
    12361 taccacaaaa aatattgctg cggaaatcat ccagaacgca atcagtgcgg gaaccagaga
    12421 cccccggttt ttaccggtag aagaaagtga gctttctgaa ttggcctatt cagtggatgt
    12481 attgggggaa ccggagccca tcaatacaaa agatgagctt gatgtgaaaa ggtatggggt
    12541 tattgtgaga agcgggtaca aatccggatt gctgcttccc aatctggaag gcgtggacac
    12601 cgttgattat caagtgagta tagcactgca gaaggcaggc atcaggcccg gcgaacctta
    12661 taaaatggag cgttttgaag tggtaaggca ttatcagtat atggagtgat tcattatggg
    12721 tcaattgaaa gaagcaaagc attatgagaa acaagaaggg ggcaatgtca aatgcaggct
    12781 ctgtcctcac gaatgtaaca ttgcaccggg caaatacgga atttgcagag taaggaataa
    12841 catcgggggt attctctata ctcataacta tggaaaaatc tcttcaattg caatggaccc
    12901 catggagaaa aagccccttt atcatttttt tccgggcaga tatatcctgt ctataggtac
    12961 tgttggctgc aatctcagat gcagcttttg ccagaactat gagattgcac agcggggagc
    13021 agctgaggag ggcggagagg ataatctgta cgaagcatct gaggcttatc tgcttagctt
    13081 atgcaggaag gaagaggatt gtataggtgt ggcatatacc tacaatgagc cgagtatatg
    13141 gtttgagtat atattggata cggcagggca tataaggtct gaaggattca aaaatgtgct
    13201 ggtcacaaac ggatatataa gcagagaagc tctttctgag ctgctgcctt atattgatgc
    13261 catgaatatt gatgtcaaag gatttacgga gaagtattac cgcaatattt gcgggggcag
    13321 ccttgagcat gtgaagaaaa cggtagaagc ggctgctgcg gaatgtcacg tagaaattac
    13381 tactcttgtt gttccggggc acaatgacag tgagagggag attcaggaac tctcaaaatg
    13441 gttgtcgtct atcaatcctt caattcctct ccatttatcc cgttactttc cgagatacag
    13501 gatgaaggag cagcctaccc cggcaggaac tctgatttct ttgaaaaaag cggccgaaag
    13561 ttatctggag tatgtgtaca taggaaatat gcaaagtgag gctgtaaata cttattgccc
    13621 gcagtgcgga gccttgctta taagaaggac gggaggcatt atcatagagc atctggatga
    13681 cggtatatgc ttcaaatgcg gtaaagctat aagtataacc ggtatttagc aatatattga
    13741 atatgccgga tagcatctgc tggttattcc tggtataatt atcctggcat atagatatga
    13801 attgcaggag tgcgggaggc gatgaaatga attttttcgt gcagatgagg gaaagcgtaa
    13861 ttgatttcaa gttttacaga agcattaggg ataataaatt cagcaggagc ttcgtttatc
    13921 tgctgctgct ttttctgata atctatttca taaatggaac cagaactttc atagtaaccc
    13981 ggatatttat ggatgatctg gctgtccatc tggctgaaaa tgttccggaa ttcaggctgg
    14041 agaacggtgt attcagcttt gaaggagata tgccctacta tatcagcagc agcaccaatg
    14101 aggcttttat tattgacact acgggacaag tgacggaaag catactggag gatgccggaa
    14161 ccggaatgct gataaccagg gacaaaatat tcgtgaagag aaacaagata gaaaccaggc
    14221 agttcagcct gtccgaactg gaaggcttta cacttgcaaa atctgatgta ttggaattta
    14281 tgccgaagct cagctggatt gttttcattt taattgcttt cagttttatt tttgtattgg
    14341 gatggaagct gcttaatgca gtcatactgg cattattggg gattgcagtc aatgctttgc
    14401 tgaaagggag gctgagatac aataatctgc tgaatatcag catatatgcg ctgacacttc
    14461 caatgcttgt tcaattagcc gttaacctat acggataccc tatacccgga ttcgggttaa
    14521 tatactggct tatttccata ctatacctgg atttggcgat aaaaagctgc atagaccagg
    14581 ataaggaatc tgaagcagga aatgaactgg aggatattaa aggtgaagat aggagtaatt
    14641 tctgatacac atataagaag cagcgcgaag ctccttccca acataatata cagtgtgttt
    14701 gacggcgtgg atgcgatatt ccacgcgggg gatatactta ttgatgaggt aataattgaa
    14761 cttgagacta ttgcccctgt ttatgcagtg gcgggaaaca acgacagtta tgaaatgctg
    14821 tataaataca gcacaaaaag aataattgag gctaacggaa aaagaatagg gctgacccac
    14881 ggaacaagca gggtaaacgc atatgcgggc gcttattcaa tgtttgcaaa gaacaatgtg
    14941 gattgtgtcg tatatggaca cagtcacaaa ccgcataatg aagtaattga cggagtatta
    15001 tttttcaacc cgggttcacc gacctcaaaa aggtttgagt ccaggtattc tttgggactt
    15061 ttgcatatag gcagggatat aagaggagaa ataatatatt ttgattgaag tgcggtacga
    15121 ggcgtttttt tatcgtatag gaaattataa tttcctatac gatgggggat gcaaggggga
    15181 gaagccccct gtcgataaag gaaggtgctc aggatgcgaa gcaaaaacga agggtactta
    15241 tttaaagccc tagcgaaaaa aatgcgccgt gagacgcgtt ttttatttgt gtatttttta
    15301 acaataatgt aaaatatgaa ttgaaacacg gcagttcttc agaaatgatt aaataaatat
    15361 tcaggaggtc attatgttag aaaagatact ggagcataac aggaaatttg tgcaggaaag
    15421 aaaggctgca ggagaagaca aacccataag cggccatgcc cataaggata taatggtatt
    15481 cacctgcatg gatacaaggc tggtggaact attggaacct gccatggggt ttggcagagg
    15541 ggatataaaa atacttaaga atgcaggcaa cattataaga aacaactgca gtgacgttat
    15601 cagaagcgta gctgtaggaa cagtactgat gggattaaaa gaagtatacg tggtaggcca
    15661 tagtgactgc ggaatgagaa agcagaaacc agaggatata agagcaaaaa tgagggaaag
    15721 aggcatacct gaggaggcaa tagaagccat agatataaag gaatggttcg gcctgctgca
    15781 ggatgaagca gaaaacgtaa aggatgcggt tctcaaactg aaaaattctc catatatacc
    15841 gaaggatgta aaaatacacg gattactaat ggatcctaat tcaggagaaa tatcggtaat
    15901 cgagtaatta tacgggagtc aagaaatctg aatttttaga actctctaga attatagaca
    15961 tattgttaaa aagatgctaa acttaaattg ttaaaaaagt acttaactta tatataccat
    16021 ttaacaaagt ttagaatgga gggtctttta gaatggagaa aaggatgttg actccaaagg
    16081 aaattgcaat ggcaacctgc gatgccggtg ccgcgaaagc aaagctctca attgcgcaga
    16141 tgatggtgct gggtatcttt gcaggtgtgt tcataggctt tggagcacag ggggcaataa
    16201 ccatcggaca gacactggca aaggttgata caggtttgca gaagtttgca tttgccggag
    16261 cattccctgt aggacttatg ctggtggtta tctgtggtgc agaactgttt acaggtaata
    16321 acctgatgac tttaggatgt ctgaactgcc gttacaaggt atcggatatt cttaggaact
    16381 ggttctttgt ctatacagca aactttattg gttcggtcat acttgcggct attattgcaa
    16441 atacgggatt gatggcggca ggaactccta ttgcagaatc tgctataaaa atcgcatcta
    16501 acaaggtcgc catacctttt ggcgctgcag tattaagagg aatactctgc aatataatgg
    16561 ttgttcttgc agtatggatg gccacaggag caaaagacgt aatatcgaag atattcgctt
    16621 gctggttccc gattatgctc tttgtactct gcggatatga gcatagtgtt gcaaacatgt
    16681 actttattcc tcttggaatg ttcttgggag caccagttac ctggggacag atttggatta
    16741 ataatctgat acctgtaact ataggcaatc tcattggtgg agcgcttatt gttccgttat
    16801 tctaccatgt agcatacatg aaagctccga cagcagcagc tccgacaaag gatgctaagg
    16861 cataagaatt gtaaaagcaa ttattatagt atataattgg acataaatat ataagttttc
    16921 cgagtcatcg cttctaaagg caaccatgaa gccacgaccg atgggcaaat cccataaggg
    16981 ttcagggaga aatccccgag cctcccgtat taggaaagga aaatatttta tgatatattt
    17041 tttgatatat tattaataac aatccttttc cggattgtta ttctttttta cagaaataat
    17101 tcacatatca taatttgacg tgtgctgttg taaaggatgg aagtcacaaa tgaaggatga
    17161 attagggaga aatataaatt atctgcgaat atccgtaaca gacaggtgca atctcagatg
    17221 ccgctactgc atgcctgagg agggcataga gaagaaggag catttggata tattgacctt
    17281 ggaggaaata tttgaagtag tgagggtatg tgctgacctg ggtacaaata agataagaat
    17341 taccggagga gagcctcttg taagaaatgg gcttacgggg cttattgaga gaataacagc
    17401 acttgaaagt ataaaggact tggctcttac caccaatgga agccttctca aaaaatatgc
    17461 ccgtgaatta aaaaaggcag gactcaaaag gataaacata agcctggata ccatggatga
    17521 taagaagtat gaatatttga ccaggggagg gaagctcaaa gatgtcctgg agggattcca
    17581 cgaggcattg gaggttgggc tgaaacccat taaaatcaac acagtgctta caaaaggctt
    17641 taatgaggat gaaatcggtg atttcataag gcttacacaa aattatgaca tcgatgtccg
    17701 atttattgag cttatgcctt tagggcatgc cgccggcttt gcatcgaagc actatatttc
    17761 caatttagtg gtgctggaaa gatataagga gctggaattg gcggaagccg ccgataaaag
    17821 cagtccggcc atgtactaca agctgcccgg cgcaaaggga agagtagggt ttattaatcc
    17881 cataagccac aaattctgtg acaactgcaa caggctgagg cttactgcag acggaaaact
    17941 gaagccctgt ttgcattcca acctggagat agatgtaaag ggaatactgc ggggaagaaa
    18001 ttccgatgac agatacctgg cattgcagaa ggcagttgaa gaagctgtac aagccaaacc
    18061 cgggcaccac acactgaatg atctttataa caagcctatt gatcgtgaca tgtacacaat
    18121 aggcggataa agaaaagggg gatatgtatg aagggaaagg tattagcagt aaatataagc
    18181 gaaaagaaag gtgtattcaa aaagcccata gaacagggag aattcaaggt tgaccacggc
    18241 cttgcagggg atgcccatgc gggaaactgg cacaggcagg tgagcttatt aggtatagag
    18301 agcataaaca agatgaaagc cttaggcatc gaggggcttt ctacgggcaa atttgcagaa
    18361 aacctgacca cagcaggcct tgagctctgg aagctccctg tcggtacaaa gctaaaaata
    18421 ggcgaaacgg agcaggaggt aacccagata ggcaaggaat gccatgtgaa gtgtgaaata
    18481 ttccagaaga taggaagctg cataatgcca acagaaggaa tatttacaaa ggttctgaag
    18541 ggcggaataa taaaacccgg cgacgaaata gaggtacagg gggacgttta agctgtcctt
    18601 gagacggaga tatgctcacc gcctgttgga ggacatcttt tcgcctcgtt ataagggtat
    18661 aagaccaaaa aataaaggag gagaataata tgcctgattt tggtaactca ttttccggac
    18721 ttgccaagga ccgtaagctg actgaccagg aattaatcag ggcaatcaga ttcatggttg
    18781 cggcagaata tgaggcaata cagctgtata tgcagctggc agaatcaaca gacaacatgc
    18841 tggcaattga agtattaaag gatattgccg atgaagaacg ggttcacgcg ggagaatttc
    18901 tcagactgct ctacgagctg gctcctgatg aagaaaagtt ctatgctgaa ggtgcagaag
    18961 aagtagaaga agagattgaa gcgttcaaaa ggaagaagta gagtgcaggg agaggatcaa
    19021 actccttatg ctgtctccag aacagtttat ttaaatagag aggacatttg tttttcatgc
    19081 tgacaaacga aatgtccttt ttttttgatc gcgaaagcat tactttccta tacaatgggg
    19141 atgtattgaa gaatatcaaa atttggcgta taaatagtgt cggattttat atgatagtgt
    19201 atttgattta gaaagcatat atcgtataat agaatataat aggtaaatgt tggcagcgaa
    19261 tctgatgcaa aagaggggat tcttttggca atttgtgtga gaaagggaaa accgggagtg
    19321 ctggagcttg tgtttccata taatgaggaa tatattgcta aagtaagggg gatacccggg
    19381 cgcagatggg ataaagacaa taagaaatgg ttggtaccgg attgtgagga gtccattaat
    19441 caattacagg agctgtttac gaaagaagaa ctggttattg tgaaaggcag ctccaatgac
    19501 cagtttctaa aaaagatgga agaaggatta aaactaaggg gatatagtca aaaaaccata
    19561 aagtcatata tgagtcatgc aagattgttt cttgggtatt gcggcaagaa tgctaaccaa
    19621 ttagtgaagg aagatattaa ttgttatctg ttatatttgt tggaacaaaa aggtaatgca
    19681 cattcatttg taaaccaagc tgtaagctca attaagttcc tgtttggaaa agtgcttatg
    19741 gaagatgatt taacgctaga catagtcaga cccaaaaaag agaaaaagct gccggaagtc
    19801 cttagccaaa atgaagtgag gcatatatta gataatgtaa ccaatttcaa gcataaggca
    19861 atactatttc ttatatattc atcagggctg cgggtgggtg aggttgtacg gcttaaactg
    19921 aaagatattg acagcgacag gatgcttatt cacgtagttc agggtaaggg gaggaaagac
    19981 agatatacag tgatcgctga agttgctctc ggtgtactgc gcgaatatgt gcagaaattc
    20041 aggccggttg aatggctgtt ccaaggggaa gaggttggca ggcatattac tgaaagaacc
    20101 gtccagcgtg tatttgaaaa cgcgaaggaa aagtcaggaa tacaaaagaa ggtatctgta
    20161 catagtttgc gacattcttt tgcgactcat cttttggagg gagggataga cttgagatat
    20221 attcaggaac ttctaggaca tcaaagcagc aagacaacag agatatatac acatgtgaca
    20281 gagaagggaa taaagaatat tgccagtccg ctggataaac taatgagtag aagggaatag
    20341 aactgaatac ctacatatgg ggtaattcac gacactcgtc gcctatcata acatattggc
    20401 atcattcacg acatatgaca ggaatgcggt agttagctga cagctcatgt acatgattgg
    20461 atggagattt gatcaataaa caaggagtga gtaaaatgaa actgaatcac gattgtatac
    20521 gagcattatt aatttactta gaagaaaact tggaagttaa ggctaatgga ttacctgaag
    20581 gcttaaaatt aggacatata gacgaagacg atctatctgg gttctcacaa gaagacattt
    20641 tttattcagc aaagaagctt gtggaagcaa aatatatcga ggttgccaac aaagaagtag
    20701 ccccaagagc gatgataatc aaggaaatta cttggaacgg gcacgactat ttagactcta
    20761 ttcgagaccc gaaagtgtgg tatgaaatta agagtagaac taaagatttg tccgggattg
    20821 cgcttgaagt agtaaagagt ttggctattg aaataacaaa gcaaatgctg ggccttggat
    20881 gacaatcgaa atactgcaat cttgtttaat ggtagtaatg ataaatcgcg ataatactaa
    20941 tgctaaacag ttacagcgaa tgataattag ggtagggctc tgtggacatg agccatcagc
    21001 taacaatgcc ctcacgctgc ggcacggcag taaaggcagg gagtagacgt gccttgggtt
    21061 gcagaagatt tgctaaactt tggtttgatt tacgcagcaa ccacacctct tcaccgggag
    21121 cgaagctccg ccagggagaa gggctctgag gtccggctca gtggtgtcgt gagtgcgaac
    21181 ccgttatacg ccatctcacg gattcattta ttcgagacta atatattgcc gtattaggaa
    21241 gttatagagt ataagagaag taccatacat atcgccacat acgctatcct ttttcttatt
    21301 atctccaggc atccgtgcct tgggtggggc agggagcttc ggtcgatatg aaattgagag
    21361 actggagaga agcaaaaaca ccgcatggta cttattggta tgatatactg tatttaggtg
    21421 gctgacgtcc gtgtcagccg ctgaagctaa
//
LOCUS       DAOQUR010000039        20551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3040_length_20551_cov_10.194916, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000039 DAOQUR010000000
VERSION     DAOQUR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20551
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3040_length_20551_cov_10.194916"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1006)
                     /gene="fabG"
                     /locus_tag="PKI50_08720"
     CDS             complement(266..1006)
                     /gene="fabG"
                     /locus_tag="PKI50_08720"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNT03821.1"
                     /translation="MRLKDKVAIVTGSTKGIGKETAMVFAWEGAKVVICGTRENAIKE
                     VTEEIKSMGAEALGFKVDVGNRAQVDEMVAEVKKVWGRIDILVNNAGITADAMLRKMT
                     EEQFDKVININLKGVYNCAQAVLDTMTEQGSGVILNASSIVGVYGNIGQTNYAAAKWG
                     VIGMTKTWAKELGRKGIRVNAVAPGFVMTPMLETVPEKVLDSLREKTPMQRLGKPEDI
                     ANAYLFLASDEASFITGTVLSVDGGLVI"
     gene            complement(1030..2205)
                     /locus_tag="PKI50_08725"
     CDS             complement(1030..2205)
                     /locus_tag="PKI50_08725"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNT03822.1"
                     /translation="MREVVIVSGARTAIGNFNGSLAGVSAVDLGVTAVKEAIKRAGIT
                     PEMVDDTIMGCILQAGLGQGVARQVAVKSGIPVEKPAYTINMICGSGLRAVQVAAQQI
                     ISGECDIVVAGGTENMSAAPYLLRNARTGYRMGHGEIIDSMIYDSLTDVFNNYHMGIT
                     AENLAEQYNITREEQDAFAASSQNKAEAAIKEGKFKDEIVPVVIPSKKGDIVVDTDEF
                     PRAGVTAEALAKLRPAFKKDGTVTAGNASGINDGAAALVVMAADKAEELGIKPLARIA
                     GYASAGVDPSIMGIGPVKAVKKALERANLKLEDIDLIEANEAFAAQSIAVARDLGFDM
                     NKVNVNGGAIALGHPVGASGARILITLLYEMKKRNVKKGLATLCIGGGMGTALVVEM"
     gene            complement(2478..3860)
                     /locus_tag="PKI50_08730"
     CDS             complement(2478..3860)
                     /locus_tag="PKI50_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03823.1"
                     /translation="MKKISYRLWVQMLLLIGITLGLLWVLQVVMLEQYYLYSQTRNIK
                     QEGIALAKAYEEGMNEELLTEKAEELNLKFNAGVEIYSINGERVFGFSLMPMMMGRAV
                     YKQEVFLEAAKGEAIVRQLKHNRLNSNILLVGIPIGEGPEIKGVMIVSAPLAAIKETK
                     EIIKEQLFIITVILILISLLLTYYSSKAFTKPILKINEAAAKMAKGNLSVRVDVESSD
                     ELGMLSDTINNLSAQLQKIEQLRKDLIANVSHEFRTPLSLIKGYAETIRDVTGDMKEK
                     RNSQLSIILEETDRLKSLVDDTLNLSQIQSEDFRPDIVPFDIFATVNSIMSRFAHMSE
                     EKGITLDMQWDKASVVVLGDEPRIEQVIYNFVNNAFNHTASGGWIKISISTNEDNVKI
                     EVSDNGHGIPSEELPYIWDRFYKASKDDKGKGLGLSIAKEIMEAHNSSYGVESIPEVG
                     TTFWFTLLKA"
     gene            complement(3857..4552)
                     /locus_tag="PKI50_08735"
     CDS             complement(3857..4552)
                     /locus_tag="PKI50_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT03824.1"
                     /translation="MSGVRILLVEDENRIREMVKEYLENEGFVIDEAADGAEALDKFG
                     NSEYDMLVLDVMIPKVDGWSVCREIRRASSIPILMLTARGEEYDKLFGFELGVDDYIV
                     KPFSPKELVARIKAVLRRSSNSGSNAYSAELFEYKGLLVNMSSRKVTIDERNIDMTPR
                     EFDLMSFFIKNRDRVFTREQLLNKVWGYEFGGDFRTVDTHIKMLRESLGEYRNLLVTV
                     WGVGYKFEAEDTK"
     gene            4766..5272
                     /locus_tag="PKI50_08740"
     CDS             4766..5272
                     /locus_tag="PKI50_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03825.1"
                     /translation="MKKALLATVLVLTLVLTSFSVFADGDQSILSKVTGLSDDEISEL
                     RTQRIGYGQLIPASVLSEMLGMDIKDVIGLRKAGKSYYQIAVDKGVTAGDYKKSLLEK
                     RNAYIDEQVKSGSITEDQGNLIKERMSVNIDGCTGQTPGAGRFGGGCGMGGGFGRGFD
                     RGFGRGSL"
     gene            complement(5339..5581)
                     /locus_tag="PKI50_08745"
     CDS             complement(5339..5581)
                     /locus_tag="PKI50_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03826.1"
                     /translation="MEYVKMPELLSAEAARVLAGNMDAVKEMMVAREQASYNGGCHDD
                     DICCPKKIKFIFVLNNVVIINVNAVAEEDNHGPSQC"
     gene            complement(5733..6233)
                     /locus_tag="PKI50_08750"
     CDS             complement(5733..6233)
                     /locus_tag="PKI50_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03827.1"
                     /translation="MDQVIMPELITPKFAEVIAENPEVLKAITDPQPGEQVIVGQNCS
                     MECGGNPTNVKLVFCNVTIGLDNASDDCNKGRCREKAEEHIEGCHIKIGDRGHEKGHD
                     DWGDISGRHKQIWMEMEILEARKNEIEKILKLIPRQIFNRKLVARCKREILEIEEQLS
                     AKRMEL"
     gene            complement(6325..6639)
                     /locus_tag="PKI50_08755"
     CDS             complement(6325..6639)
                     /locus_tag="PKI50_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03828.1"
                     /translation="MGYVKMPGMLSPELVKALAGNVHTVKEEPSPKDEMAAALKDFFK
                     ADKKERKYEDNECAQPKHKARRVIDDDEICCPKKVKFIFLLNNAVVINVNALAEENNT
                     PG"
     gene            complement(6785..7198)
                     /locus_tag="PKI50_08760"
     CDS             complement(6785..7198)
                     /locus_tag="PKI50_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNT03829.1"
                     /translation="MKTIIKGKSLVGGSTEGTLIVSHEPFSFFGGFSFKTGSIVDTRS
                     DVYQEVLKGKIFAFPCGKGSSSTAGVLLEALRKGIAPRAIININSEKILAIGSIVAEK
                     MYGLNLPIISISEDDYQLLKTGLFLRINNEIIEVM"
     gene            complement(7226..8503)
                     /locus_tag="PKI50_08765"
     CDS             complement(7226..8503)
                     /locus_tag="PKI50_08765"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HNT03830.1"
                     /translation="MMIELLTEVKNLKLTQDEQEILSGKRGEAAKIALEIIIELGELY
                     GAAELIPVSKAHIDGCLYGAVGEAGLEFAEKMHQMGGRVAVPTTLNANSRDINCWVNH
                     RMNAEFAEKNARMEKAYLGMGAIPTWTCAPYQGVCAPRFGEQVAWAESNAIVYVNSVI
                     GARTERYGDYTDLCCALTGRAPKFGLHIKKNRLAQIIINCEGLPLKSSTDDFAVLGYL
                     VGKLAKDKIPAITGIPQWVTSDQLKAFSAAIASSGAVAMFHMIGITPEAPDLNTISDD
                     PSNIETIEITKEMYLDSRKSLSSATPESADAILIGCPHASAAELMEIADLLNGRFIPE
                     EMEFWIYTNREVYSLLESTLIIDNLIKSGVRIIRDTCFLNTDLSGWNMKTVMTNSAKY
                     AHYMKALTGREVVFANLGECINTALQSKGQPRI"
     gene            complement(8798..9826)
                     /locus_tag="PKI50_08770"
     CDS             complement(8798..9826)
                     /locus_tag="PKI50_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03831.1"
                     /translation="MKTLKLCMIGFGNVGGEFCRLLEEKKAALLENNNCRVIITEIAT
                     KSRGILRNVNGIDTDGALDWIEKKGSFKGHEDYINLNSEEVIKSTTADALLELSTLSV
                     MDGQPAIKHIETAFDRNMHVITANKGPIAWRFKKLDTRAKEENVVFLYETVVMDGTPI
                     FNLVRNTLQGCRVLSFRGILNTTTNYILCEMEKGNSFEEAVKEAQRRGFAEADPSLDV
                     DGWDAAAKTAALMNVLMDAEVTPLDIKREGISGVIAADVLRVKQEGSCIKLICEGFFE
                     NGISVGRVYPKEIDESSIYSKIDATSSVLSVTTDLMGEISIVEHDPEIRQTAYGVYSD
                     LLALLGRI"
     gene            complement(10009..10242)
                     /locus_tag="PKI50_08775"
     CDS             complement(10009..10242)
                     /locus_tag="PKI50_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNT03832.1"
                     /translation="MPYIAIKSFPKDQATKEAVAEKINEIFLEVWGCPQEAITISIED
                     ITPADWEEKIVKTEIDPKKENMMILSGKRCYKR"
     gene            complement(10549..13191)
                     /gene="ppdK"
                     /locus_tag="PKI50_08780"
     CDS             complement(10549..13191)
                     /gene="ppdK"
                     /locus_tag="PKI50_08780"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNT03833.1"
                     /translation="MKKYVYNFKEGKASMKDLLGGKGANLAEMSNIGLPVPPGFTITT
                     EACNEYYNKGEQLWSELIDEIMEKLTDLEATTGKTFGGGSNPLLVSVRSGAKFSMPGM
                     MDTILNLGLNDEVVKTVKDITDNERFAMDSYRRFIQMFSDVVMEIPKSKFERVLDEIK
                     EEKGAKYDTDLTASDLKEVVEKFKSIYKAEKGEGFPQNPKDQLLLAVTAVFRSWNNER
                     AIIYRAMNNIPGNLGTAVNVQSMVFGNMGNDCGTGVAFTRNPATGEKKIFGEFLMNAQ
                     GEDVVAGIRTPQTIEKLSEINQEVYDQFISVANLLENHYRDMQDIEFTIEKGKLFMLQ
                     TRNGKRTTAAALRVAVELVEEGKITKEEAVLRVEPAQLDQLLHPNFDSAALKKALSIA
                     KGLPASPGAASGKAYFTAADAVEASKNGEKVILVRLETSPEDIEGMAMAKGILTARGG
                     MTSHAAVVARGMGKCCVAGCEGLKVSEELKQFVANGKVYKEGDYISLNGSTGYVYEGV
                     IPTKEPEMSGHFATLMSWADEVRRLKVRTNADTPKDAEQAVKFGAEGIGLCRTEHMFF
                     SEDRIAAVREMIISETVEQRKVALAKLLPMQREDFIGIFEAMEERPVTIRLLDPPLHE
                     FLPQEDEDIKELAKTMDIEFGKLKARVESLHEVNPMLGHRGCRLAVTYPELAEMQARA
                     IIEAAVEVKKNKGIDVHPEIMIPLVGELKELEYIEKVVRQTADKVIAESGIKLEYLYG
                     TMIEVPRGAVTADEIAKTAEFFSFGTNDLTQMAYGFSRDDAGKFIEEYLKKGIFDQDP
                     FQTLDQNGVGKLVKMAVDLGRKTRPDIKLGICGEHGGDPASVEFCHRVGLNYVSCSPF
                     RVPIARLAAAQATIKDKMGEYSQK"
     gene            complement(13188..14006)
                     /locus_tag="PKI50_08785"
     CDS             complement(13188..14006)
                     /locus_tag="PKI50_08785"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNT03834.1"
                     /translation="MNKNRAVIFVISDSIGETAEQVAKAASAQYPECEIRIRWIPYVT
                     EIESIQEVISEAKDLDSIIMFTLVVPELRKYLTEQAAMHNIICVDIMGPAVEAIAKVT
                     GQHPTDIPGAARKLDDEYFSKIEAVEFAVKYDDCKDPRGIKKADLVLLGISRTSKTPL
                     GMFLATKNLKVANIPLMPEVKPPKELFEISPKKIIGLVISTDQLNPIRLERLKSHGLS
                     DQANYASKERIAYEHKYAEEIMNKIGCRIVDVTDKAIEETANTILQILKEAEQE"
     gene            complement(14021..14662)
                     /locus_tag="PKI50_08790"
     CDS             complement(14021..14662)
                     /locus_tag="PKI50_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT03835.1"
                     /translation="MVAIEFTSRQKQIIELVKKYQPITSEQLAAKLNMTRAALRPDLT
                     ILTMSDMLEARPKVGYFYSGKIINDIGHDFIKSIKVAEIKSSAVVMDESATVYDSIVM
                     LFLRDAGTIYVQSKGALSGVVSRKDFIKIAIGNNDIQNMPIGMIMSRMPNIVYIFDND
                     SAYDAVVKLITHEIDSIPVVEPFKDSDGKEQFKITGKVSKTNMTKLLYDLYKQ"
     gene            complement(14793..16877)
                     /gene="glyS"
                     /locus_tag="PKI50_08795"
     CDS             complement(14793..16877)
                     /gene="glyS"
                     /locus_tag="PKI50_08795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNT03836.1"
                     /translation="MSKDLLFEIGTEEIPANYMPSTLKQVKSISGAMLKNYRIAFEEI
                     KAYGTPRRIVLFVKGIAEQQENLEELVKGPSRRAAYDENGNPSKALLGFLKGRKAELG
                     DVFIQELSGVEYVYYKKQEKGQPVKKVLKTILPDILTSISFPKSMKWGNKSFRFARPV
                     RWLVPILGDELIKFDKDGIKCSRYTKGHRVLSKGSIKINNTAEYFDKLKAGFVIADQE
                     ERRAIIRRQCEELAREKGGEVLIDEELLEEVVYLVEYPTALIGGFDAEYLKLPKEAVI
                     TPMKEHQRYFPVVDNDKNLMNYFITVRNGDSRFLETVREGNEKVLKARLADADFFYSE
                     DRKESLDNCAEKLKNVVFQETLGTIYDKTQRISAIAEYLAAILGLNAGAKKNLLRSAH
                     LCKADLVTNMVKEFDELQGIMGREYALLQGEDREVADAIFEHYLPRFAGDCTPETLNG
                     SILGISDKIDTICGCFAIGIQPTGSQDPYALRRQAIGVTSIILDSNIHLGLAQLIHNS
                     LKPFEEKGILKGDEKSVKRDILEFFRQRLKNVMIDKGFEYDVIDAVINAGFDDIYDSY
                     LKILELSKWKYKDEFMNILGSFNRVSNLASKGKSTEINELLFTEKAELKLLHDYDKVY
                     AKFVNCVDNRDYGKALELMITLKKPIDDFFDNIMVMVEDEKIRNNRLGLLKSIGDMMN
                     RIADLSAVVMNK"
     gene            complement(16870..17766)
                     /gene="glyQ"
                     /locus_tag="PKI50_08800"
     CDS             complement(16870..17766)
                     /gene="glyQ"
                     /locus_tag="PKI50_08800"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNT03837.1"
                     /translation="MTFQEIILTLQKYWADRHCIIAQPYDVEKGAGTMSPYTFLKALG
                     PEPWRIAYVEPSRRPADARYGENPNRLYQHHQFQVILKPSPDNVQDLYLGSLKALGID
                     PVKHDIRFVEDNWEAPTLGAWGLGWEVWLDGMEITQYTYFQQVGSIDCQLESAELTYG
                     LERIAMYIQDLNNVFDIEWVSGINYGDVFKTAEFEHSKYSFEEADTKLLFTLFDEYEA
                     EAKRLLDIGLILPAYDYVLKCSHAFNILDARKAISVTERTGFIGRVRNLARLVAQGYL
                     KQREELGYPLLKKAVFGGEKNE"
     gene            complement(18211..18813)
                     /locus_tag="PKI50_08805"
     CDS             complement(18211..18813)
                     /locus_tag="PKI50_08805"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNT03838.1"
                     /translation="MPRSKFINVLKGVSSMDISLEKVDIIRDRTGVTYKEAKEALEAA
                     NGNVVDALINIEEAGDKRWTETISVKGSEAVEKLKGIVKSGNVNRIRVKKDDYVILDI
                     PVTAGAISAVVIPQLTAIGTAVALLSKCVIEVERPNKQTINVSEAITNTADNLAGKIK
                     NAADDFRNMTSSEAGHNSAAADASDIFGAVNDNTDNTFKQ"
     gene            complement(18800..19576)
                     /gene="recO"
                     /locus_tag="PKI50_08810"
     CDS             complement(18800..19576)
                     /gene="recO"
                     /locus_tag="PKI50_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNT03839.1"
                     /translation="MPISKTEGIVLRYTNLGEADKILTILTRNNGKIKAIAKGCRKPK
                     SSLLSSSEVFVFSEFVLYKGTNFYHISQAVTRETFYNIRKDLLRLSYATYFAELAEAV
                     SDEGIPSERLFLLLAKTLYYLSTENIPIGILGVGYQLKLMDISGYRPNIARCAICRKS
                     SEDYTRFSVDLGGVICGSCSTGENQKNAGEVFRISQGTAEAINFLLNIEISRLNTKKI
                     DNTIFNEIEKITRSFIQSHLDKRFKSLDFLDSMKDLNAQK"
     gene            complement(19581..20483)
                     /gene="era"
                     /locus_tag="PKI50_08815"
     CDS             complement(19581..20483)
                     /gene="era"
                     /locus_tag="PKI50_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNT03840.1"
                     /translation="MNTKFKSGFVTIIGRPNVGKSTLINKLLGERVSIISSKPQTTRN
                     SIRCIYTTSEYQIVFIDTPGIHKPRHMLGEYMVKTATESLKGVDVVVVMFDCSAEVGP
                     GDMYAAQVAEKVNSPVILVLNKIDKIPKSRLEQIAASLIMHEDYFDSIIPVSASTGEN
                     LDELLKEIVKHLPEGPRYFPEDIYTDMPEKFIVSEIIREKVLELTSEEVPHGTAVEIT
                     SMKERENKDLMDIAATIYCEKESHKAIIIGKQGRMLKEIGVRSRVDIENLLHCRINLQ
                     LWVKVRRNWRDSRSALKNFGYNWE"
BASE COUNT         5155 a         5218 c         3570 g         6608 t
ORIGIN      
        1 agcttcgtac cgggtctgga gcttacaaaa gctgaaacag cagggatgct cataagctat
       61 cttaaggtgg ataagacacc aaaatcatcg gaaccaaccg tagggacacc agcaataatg
      121 ccggcggcag aataaagccg aaaagaagga cacggggacg tttctcctgt cccggaagga
      181 cacggggacg tttctcctgt cccgctgaaa gcgggacagg agaaacgtcc ccgtgtcctt
      241 cttttcgtgt ccttttttat gtaaattaga ttacaagccc tccgtctacc gaaagtacag
      301 ttcctgttat aaagcttgct tcatctgatg ccaggaacag atatgcattt gcaatatcct
      361 ccggtttacc aagcctctgc atgggggttt tttccctgag tgaatccaga accttttcag
      421 gaacagtctc aagcattgga gtcattacaa agccgggggc aacggcatta acccttatac
      481 cctttcttcc aagctccttg gcccaggtct ttgtcatacc tataactccc cacttggcag
      541 ctgcatagtt tgtctggcct atgttcccat atacccctac tatggaggaa gcattgagta
      601 ttacgccgct tccctgctcc gtcattgtgt caagtacagc ctgagcacag ttgtatacgc
      661 ccttcaggtt gatattgata actttgtcga actgctcttc cgtcatcttc ctaagcattg
      721 catccgctgt tatgcccgca ttgttaacaa gtatgtctat tctgccccaa accttcttaa
      781 cttccgcaac catttcatca acctgtgccc tgttgcctac gtctaccttg aagccaagag
      841 cctctgctcc cattgactta atctcttctg tgacttcttt aattgcgttt tcgcgtgtac
      901 cgcagattac cacctttgcg ccttcccagg caaagaccat tgcggtctcc ttgcctatcc
      961 cttttgtaga acctgtgact atggctactt tatccttaag cctcatatac tttcctcctt
     1021 ttttgtatac tacatttcca ctacaagtgc ggtacccata cctccgccta tacataaggt
     1081 tgcaagtcct tttttcacgt ttcttttctt catctcataa agaagtgtta taagtatcct
     1141 tgcgccgctg gctcccaccg gatgtccaag tgcaattgcg cctccgttga cattcacctt
     1201 gttcatgtca aaacccaaat cccttgccac tgcaatagac tgagctgcaa aagcctcatt
     1261 ggcttcgata aggtctatat cctccagctt gaggttcgct ctttccaaag ctttctttac
     1321 tgccttaacc ggccctatac ccattatgct tggatccact ccggcagatg cataacctgc
     1381 tattctggca agaggcttta tgccgagttc ttccgcttta tccgcagcca ttacaaccaa
     1441 tgccgcggca ccgtcattta tacctgaagc atttccggct gtaacagtac catccttttt
     1501 aaacgcaggc ctcagcttcg ccaaggcttc tgcagtaact cccgctcttg ggaattcgtc
     1561 ggtatcaacc acaatatcac ccttttttga aggaatgacc accggaacta tctcgtcctt
     1621 gaatttgcct tccttaatag ctgcttcggc tttgttctgg ctggaagcag caaaagcatc
     1681 ctgctcttct ctggttatat tgtattgttc cgccaggttt tcggcagtaa tgcccatatg
     1741 gtaattgtta aatacatcag tgagggagtc gtatatcatt gaatctatta tttctccgtg
     1801 acccattctg tagcccgttc tggcatttct gagcaaataa ggagctgcgc tcatgttctc
     1861 agttccgcct gcaacaacaa tgtcgcactc tccggatatt atctgctggg cagcaacctg
     1921 tacagccctc agacccgaac cgcatatcat gtttattgta taagcaggct tttcaaccgg
     1981 tatccctgac ttcacagcta cctgacgtgc cactccctgg ccaaggcctg cctgaagaat
     2041 acatcccatg atggtatcat ccaccatttc aggagtgatc cctgctcttt taattgcctc
     2101 cttgactgca gtcaccccaa ggtctactgc ggaaactccc gccaggctgc cgttgaaatt
     2161 gcctattgct gttcttgccc cacttacgat aacaacttct ctcatcgtgt ttaatacctc
     2221 ctcaaagaat taattactat tcgtataaaa gcaatgcaac ttttatgcca aattcccttc
     2281 agccctatgt gacatagaaa acgagcaaca cggaacattt atatattgtg cagctgcatc
     2341 agaaaacttc gataatcact tcattgccgt tcattattca tgattcaaaa attatcgccc
     2401 tccgtgcatt cagtggtttt ctgtgttttc agtgttatcc gtatctagtt cccttttcgc
     2461 ctaaaattgg gcatatatta cgctttcaaa agagtaaacc aaaatgttgt tcccacttcc
     2521 ggtatacttt ctactccata gctgctatta tgtgcttcca tgatttcttt ggctattgaa
     2581 agccccagtc cttttccttt gtcatccttg gatgccttat agaatctatc ccaaatatat
     2641 ggaagttctt ctgatggtat gccatgaccg ttatctgata cttcaatttt tacgttatct
     2701 tcattcgtcg aaattgaaat ttttatccag cctccggaag ctgtatgatt gaatgcatta
     2761 tttacaaaat tgtatattac ctgctcaata cgcggctcgt ctcccaacac aacaacgctg
     2821 gccttatccc actgcatatc cagggttatc cccttttcct ccgacatatg tgcaaacctg
     2881 ctcataatgc tgtttactgt cgcaaaaata tcaaaaggaa ctatgtccgg cctaaaatcc
     2941 tccgactgaa tttgggacag attcaaagta tcatctacca gactctttaa cctgtcagtc
     3001 tcttccaata taatgcttaa ctgactgttt cttttttcct tcatatctcc cgtaacatcc
     3061 cttattgttt ctgcatatcc cttgatcagg ctaaggggag tcctgaattc gtgggacaca
     3121 tttgctataa gatccttcct cagctgctca attttctgaa gctgcgctga aaggttattt
     3181 attgtatctg aaagcatccc aagctcatct gagctttcta cgtcaaccct gacggataga
     3241 ttgcctttag ccatttttgc tgcagcttca ttgattttca gtataggttt tgtaaaggct
     3301 tttgatgaat agtatgtaag cagcagagat atcagaataa gtatgactgt aattataaat
     3361 agctgctcct taataatttc cttagtctct ttaatcgcag caaggggcgc actgacaatc
     3421 atcactccct ttatctcagg cccttctcct atcgggattc cgaccagcaa aatattgctg
     3481 ttgagcctgt tgtgctttag ctgtctgact attgcttcac ctttagcagc ttccaaaaat
     3541 acttcctgct tatatacagc cctgcccatc atcattggca tgagagaaaa tccaaatacc
     3601 cgctctccat ttatggagta tatttctacg ccagcattaa acttaagatt taattcctca
     3661 gccttttctg taagcagttc ttcattcatt ccttcttcat aagcctttgc cagtgcaatt
     3721 ccctcctgct tgatatttct ggtctggctg tacaagtagt actgttccag cataacaacc
     3781 tgaagaaccc aaagtaatcc gagggtaatc ccaataagca gcagcatctg aacccataac
     3841 ctgtaagata tctttttcat tttgtatcct ccgcctcaaa cttataccct actccccaga
     3901 ccgttaccaa aaggttcctg tactctccca ggctctctct cagcattttg atatgggtat
     3961 ctaccgtcct gaaatctccc ccgaactcat agccccatac tttgttcaaa agctgctctc
     4021 ttgtaaaaac cctatccctg ttttttataa aaaaactcat caggtcaaac tctcttggtg
     4081 tcatgtctat attcctttca tctattgtaa cctttctgga actcatattc accaaaagcc
     4141 ctttatattc gaacagctca gcggaataag cgttactccc ggaattactg ctgcgtctca
     4201 gtactgcctt aattctggca acaagttcct tagggctgaa tggttttaca atgtaatcat
     4261 caacaccaag ttcaaatcca aacagcttgt catattcttc ccctctggct gtcagcatca
     4321 atataggtat ggaggaggcg cgtcttattt ccctgcatac gctccagcca tccaccttgg
     4381 gaatcattac atcaagcacc agcatatcat attctgaatt tccgaactta tccagggctt
     4441 ctgccccgtc tgcggcttca tcaataacga acccctcatt ctccaggtat tcctttacca
     4501 tttccctgat tctattctca tcctccacca gaagaatcct tacacctgac ataacagcac
     4561 caccttactt ggtttgtatc ccactccggt attatttaat gatattatta cacaaattga
     4621 cattcaatgt cactacataa tcctgtggtt tttcaatttt cataaatcca acacaatttc
     4681 atcattatat agacaaatct ataatgtaga ataaaatcaa ggcaataata tgaagcctga
     4741 aactattaaa gaaagggtga aaaaaatgaa aaaagcgcta ttagctactg tattggttct
     4801 tacgttggta ttaacatcat tttcagtttt tgcagatggt gatcaaagca tactatcaaa
     4861 agtcacagga ttgagcgatg atgaaatatc agagctgaga actcagagaa tcggttatgg
     4921 acagcttata ccggcatctg tgctttcaga aatgcttggc atggacatta aagatgtaat
     4981 aggactgagg aaggcaggaa agtcatacta tcagatagct gttgacaaag gagtaactgc
     5041 cggggattac aaaaaaagcc tgcttgaaaa gagaaatgct tatattgatg agcaggttaa
     5101 gtcgggaagc atcacagaag atcagggcaa cctcataaag gaaagaatga gcgtaaacat
     5161 tgacggctgt accggtcaga ctccaggggc aggcagattc ggcggtggat gcggtatggg
     5221 cggaggcttc ggcagaggct tcgacagagg attcggccgc ggctccctat aacttcaaaa
     5281 aggataaatg gcccttgcca tttatccttt ttgtggttct gaacttatct gacacagctt
     5341 agcattggga aggaccgtga ttgtcttcct cagccactgc attaacattg attatcacaa
     5401 cattgttcaa aacaaatatg aacttaatct ttttggggca gcagatatca tcgtcatgac
     5461 agccaccgtt atagcttgct tgttcccttg caaccatcat ttcttttaca gcatccatat
     5521 ttccggcaag cacccttgct gcttcagctg acagtaattc cggcatcttt acatattcca
     5581 taaattcacc tcctttcatt ttctgggcgg agaatttgaa ccggttcgtc ttcttccaat
     5641 agcattatat gacttgtaca ttttattgct actgattgta caataaactc tatcaacatt
     5701 ttctcacaat atcccatact ccataaacca agttacagct ccatcctttt agctgacaat
     5761 tgctcctcta tttcgagtat ctctctttta caccgggcaa ccagtttcct gttaaatatc
     5821 tgtcggggta tcagtttcag gattttttca atttcgtttt ttcttgcttc aagtatttcc
     5881 atctccatcc atatctgttt atgtctgcct gaaatgtctc cccagtcgtc atgccctttc
     5941 tcatggcccc tgtcacctat ctttatatga cagccttcaa tatgctcttc cgccttttcc
     6001 ctgcagcggc ctttattgca atcatctgat gcattatcca gaccaatagt aacgttgcaa
     6061 aacacaagct tgacattcgt cggattacct ccgcattcca tactacagtt ctgcccgacg
     6121 ataacctgct cccccggctg aggatcggta atcgctttca gcacttcagg attttcagct
     6181 atcacttctg caaacttcgg tgttattaat tcaggcataa taacttgatc catgttttca
     6241 tcacctccct tgctggattc tgctatgtat agtaaagcat aggtacaata attgaattat
     6301 gtacctatgc atttcatatt atctttagcc cggagtgtta ttttcttcag ccaatgcgtt
     6361 gacattaatc actactgcat tattcaacag aaatatgaac ttgacttttt taggacagca
     6421 gatttcatca tcgtctatta cccttcttgc tttatgcttt ggctgtgcac attcattgtc
     6481 ttcatatttt ctttccttct tgtctgcttt gaaaaaatct ttcaaggctg ccgccatttc
     6541 atctttgggg cttggttctt ctttaacagt gtgcacattg cctgccaatg cttttaccaa
     6601 ttcaggcgaa agcatacccg gcatttttac atatcccatg gattcacctc ccctcattaa
     6661 attgacagag attcagaacc ggtttgtctt cttccaatag cattatatga cttgtacatt
     6721 ctattgctac aattcataaa aaaaaacgcc tcacggcgtc aaaacttgta ttgtttgcct
     6781 tgtttcacat tacttcgatg atttcgttat taatacggag gaataaacct gttttcagca
     6841 gctgataatc atcttcagaa atacttatga tgggcagatt caatccatac attttctctg
     6901 ctacaataga accaatggct aaaatttttt cactattaat attaattatt gcccgggggg
     6961 cgatcccttt tcttaaagcc tcaagaagca caccggctgt actgcttgat ccctttccac
     7021 agggaaaggc aaaaattttg ccttttaata cctcttgata aacatcgctt ctggtatcta
     7081 caatactgcc tgtcttaaaa ctaaaaccgc cgaaaaagct aaagggttca tgggaaacaa
     7141 tgagagttcc ttccgtactg cctcccacaa ggctttttcc tttaataatt gttttcattt
     7201 catttctccc gtataattat tttaatcaga tccggggctg acccttggat tgaagagccg
     7261 tattaataca ttcacccaaa tttgcaaata caacctccct accggttaat gccttcatat
     7321 aatgggcata tttggctgag tttgtcataa ctgttttcat attccaaccg ctaagatcag
     7381 tatttaaaaa gcaggtatcc cggataattc tcacaccgct ttttatcaaa ttatcgataa
     7441 taagggttga ttccaacagg gaatatactt ctctattggt ataaatccaa aattccatct
     7501 cttcaggaat aaacctgcca ttaagtaaat ctgcaatttc cattagctcc gctgcggaag
     7561 catgagggca cccaattaaa atggcatctg cactttcggg agtcgcgctg cttaaagatt
     7621 tcctggaatc taaatacatt tcttttgtaa tctctatagt ttcaatattg gaaggatcat
     7681 ctgatattgt atttaaatcc ggtgcttccg gcgtgatccc aatcatatga aacatagcta
     7741 ctgcccctga tgaagcaata gccgcggaaa atgctttcag ctgatctgat gttacccatt
     7801 gtggaattcc ggttatagcg gggattttat ccttggcaag cttacccaca agatacccca
     7861 atacagcaaa gtcatcagtg cttgatttta agggtaaccc ctcacaattt ataatgatct
     7921 gagccaggcg gtttttcttg atatgtagtc caaatttggg tgcccggccg gttaatgcac
     7981 aacaaaggtc ggtgtaatct ccatatcgtt cggttctggc accaatgaca gaatttacat
     8041 atactatggc attcgattct gcccaagcca cttgctcccc gaatcgaggt gcacaaaccc
     8101 cttggtaagg agcgcaagtc caggtaggga tagcgcccat tcctaaataa gctttttcca
     8161 tcctggcatt tttttctgca aactctgcat tcattctatg gtttacccag caattgatgt
     8221 ccctggaatt ggcattaagg gttgtaggta ccgcgacacg cccccccatt tgatgcattt
     8281 tttctgcaaa ctccaagcca gcctcaccaa cggcgccata aaggcagccg tcaatatgcg
     8341 ccttagaaac aggaatcagt tccgcagccc catacaattc acccaactca atgatgattt
     8401 caagagcaat ttttgctgct tcgcctctct taccggataa aatttcctgt tcatcttgcg
     8461 ttaatttcaa atttttcacc tctgttaata attctatcat cattattatt ggtcttttca
     8521 ttggtctttt cgctattctc tatttctttc cctcattcta gtaattatta acacaacaac
     8581 tttctattct ttcatagtgt taataatcac cacattgatt cagtgcctcg catgctcaag
     8641 aagggatata cgttacgagg cactagtatg tatgttccaa caaaggtctt gccgttaact
     8701 tcgggagaac agttatttcc ggtaaagaag tgggcatatg gtgtggtgcc aggcacctat
     8761 tacttattga acagcgcctt tcatcgctcc ttatttttta tatcctgcca agtaatgcaa
     8821 gcaggtcgct gtatacacca tatgcagtct gccgtatctc cggatcatgc tccacgatac
     8881 ttatttcacc catcagatcc gtagttaccg acagtaccga tgaggttgca tctatcttgc
     8941 tgtatatgct gctttcatca atctcctttg gatatacccg tccaactgag ataccattct
     9001 caaaaaagcc ttcacatata agctttatgc agctcccctc ctgctttact ctcagcacat
     9061 ctgctgctat gacgcctgat atgccttccc tctttatatc aagaggcgtc acctctgcat
     9121 ccatcagcac attcatgaga gcagcagttt tggcagccgc atcccagcca tccacatcaa
     9181 ggctggggtc tgcttccgca aagcctctcc tctgcgcttc cttcacagct tcttcaaagc
     9241 tgttgccttt ttccatctcg caaaggatat agttcgttgt ggtattgagt atgcccctaa
     9301 aggatagcac cctgcagccc tgcaatgtat tcctgactag attgaatatt ggagtaccat
     9361 ccatgacaac agtctcgtac aaaaatacca cattttcctc ttttgccctg gtatcaagct
     9421 ttttgaagcg ccatgctata ggccctttat ttgcagttat tacatgcata tttctatcaa
     9481 aagcagtttc aatatgcttt atggcaggct gaccgtccat taccgacaaa gtgctgagtt
     9541 ccagcagcgc atcagcagta gtacttttta ttacttcttc agaattcaag ttgatataat
     9601 cctcatgtcc tttaaagctg ccttttttct ctatccagtc caatgctccg tcagtatcga
     9661 taccatttac atttcttaat attcctctgc tttttgttgc aatttcggtg ataatgactc
     9721 tgcaattatt attttccaac agcgccgcct tcttttcctc cagcagcctg cagaactctc
     9781 ccccgacatt gccgaagcct atcatgcata gcttaagtgt tttcatatac agcgctctcc
     9841 tccttactct attatatttc atacagcagc tattcacaat cttcaccctg gcacctatta
     9901 ttttaagagc cttcatcctg tgttgattat ctgttttttc acattatgga acacttctgg
     9961 agaagctact ttacaaattg gaatttgtaa gcacgtgaag tcaaatcatc accgtttata
    10021 gcatctttta ccagacaaga tcatcatgtt ttccttctta ggatcaatct cagtcttgac
    10081 aatcttctcc tcccaatccg ccggtgtgat atcttcaatt gaaatggtga ttgcttcctg
    10141 cgggcagccc cagacctcca aaaaaatctc gttgattttc tcggcaaccg cttccttcgt
    10201 cgcctgatcc ttggggaatg acttaatcgc aatataaggc atactaaaat ctcctttcaa
    10261 atttaatgaa gcagcgggga agcagcgggg acggttcttt ctgcttttct ttctgctttt
    10321 tcaaatatca tattaccttg tttgaataaa gaaaagtaaa aggaaccgtc ccttttgctt
    10381 tcccatgggc attactacag tattcttgcc ctggtttgct tgagtcttag catgaaatat
    10441 gctgaaacac cgacatcatt ataccgcatt taatcaagct tttcaaaaaa aaacaaggga
    10501 agccatgtca gcctcccttg tcatatacac tggctttatg aatttctctt acttctggct
    10561 gtattcaccc attttatcct taatggttgc ctgtgctgct gctaaccttg ctatcggcac
    10621 tctgaaaggt gaacaggata cataattaag cccaacccta tggcagaatt caacggatgc
    10681 agggtccccg ccgtgctcac cgcagatacc cagcttaata tccggcctgg tcttcctgcc
    10741 aaggtctacc gccatcttta ccagctttcc aactccattc tgatcgagag tctggaatgg
    10801 atcctggtcg aatatgcctt tttttagata ctcctctatg aacttgcctg catcgtccct
    10861 tgagaagcca taggccatct gtgtaaggtc atttgtaccg aaggagaaga attcggctgt
    10921 ctttgctatt tcatcagcag tcacagctcc tctgggtacc tcaatcattg ttccgtacag
    10981 atactcaagc ttgattccag attcggctat aaccttatcg gcagtctgcc ttacaacctt
    11041 ctcaatatac tcaagctctt tcagttcacc taccaaaggt atcattattt caggatggac
    11101 atctatgccc ttattctttt taacctcaac tgcagcttca attattgccc ttgcctgcat
    11161 ttcagccagt tcgggatatg ttaccgccaa tcggcagccc ctgtgcccca gcatcgggtt
    11221 gacttcatga aggctttcaa cccttgcttt aagctttcca aattcgatat ccattgtctt
    11281 tgccagttct ttgatatctt cgtcttcctg gggaaggaac tcatggagcg gcggatccaa
    11341 aagccttatg gtaacaggcc tttcttccat tgcttcaaat attccgatga aatcctctct
    11401 ctgcattgga agaagctttg ccaatgcgac ttttctttgc tccactgtct cggatataat
    11461 catttctctt accgctgcta ttctgtcttc actgaagaac atatgctcag tacggcaaag
    11521 gccgattcct tctgctccga atttcacagc ctgctccgca tccttcggtg tatctgcatt
    11581 agtcctcacc ttaagccttc ttacttcatc agcccaggac ataagagttg cgaaatgtcc
    11641 tgacatttcc ggctcttttg tcgggataac cccctcatag acataaccgg tgctgccgtt
    11701 taaggatatg tagtctcctt ctttgtatac cttgccgtta gccacaaact gcttcaattc
    11761 ttcacttacc tttaaacctt cgcagcccgc tacgcagcac ttgcccattc ctcttgccac
    11821 aacagctgca tgagaggtca ttcctcctct tgcagtcagt attcccttag ccattgccat
    11881 tccttcaata tcttcaggag atgtttcaag ccttacaagt attacttttt cgccgttttt
    11941 tgatgcttca actgcatcgg ccgctgtgaa gtatgctttc cccgaagcag ctcccggtga
    12001 agccggaagt ccttttgcta ttgataatgc ctttttgagt gcagctgagt caaagtttgg
    12061 atgcaacagt tggtcaagct gtgccggctc tactctcaat accgcttctt ccttggttat
    12121 tttgccttcc tcaacaagct caacggcgac tctgagagct gcagcagttg tcctcttccc
    12181 gtttcgtgtc tgaagcatga aaagcttgcc cttttctata gtgaattcta tatcctgcat
    12241 atctctgtaa tgattctcca ggagatttgc cacacttatg aactggtcat aaacctcctg
    12301 atttatttcg ctgagctttt caatggtctg aggtgtcctt atccctgcga caacgtcttc
    12361 accctgtgca ttcatcaaaa attctccgaa tatcttcttt tctcccgttg caggatttct
    12421 tgtgaaagca acacctgttc cgcagtcatt ccccatattt ccaaatacca ttgactgtac
    12481 attaacagcc gtacccaggt tcccgggtat gttgttcatg gccctgtata ttattgctct
    12541 ttcattattc caggacctaa aaactgctgt tacagccagc agcagctgat ccttcggatt
    12601 ctgaggaaat ccctcgccct tctccgcctt gtagatactc ttgaacttct ctactacttc
    12661 cttcaagtcg gaagctgtaa ggtcggtatc gtattttgca cccttttctt cctttatttc
    12721 atcaagtact ctttcgaatt ttgacttggg aatctccatt acaacatcgc tgaacatctg
    12781 tatgaacctt ctgtagctat ccattgcaaa tctttcattg tcagtgatgt cttttacagt
    12841 cttgacaacc tcatcattca aaccaagatt gagaattgta tccatcatgc cgggcattga
    12901 aaacttcgca ccggatctta ccgatacaag cagaggattg cttcctccgc cgaaagtctt
    12961 tcccgttgtg gcttcaagat ctgtaagctt ttccattatc tcatcaataa gttcgctcca
    13021 aagctgctcc cctttgttgt aatactcatt gcaagcctct gtagttatag taaaccccgg
    13081 aggtaccggc agccctatat tgctcatttc cgccaaattg gcacccttgc ctcccaagag
    13141 gtctttcatt gaagcttttc cttctttaaa attataaaca tacttcttca ttcctgttct
    13201 gcctccttta atatttgaag tattgtattt gctgtctcct caattgcctt atcggttacg
    13261 tcaactattc tgcaaccaat tttgttcatg atttcctcag catatttatg ctcataggct
    13321 attctttcct tgctggcata attcgcctgg tcgcttagcc catgagattt caacctttcc
    13381 agtcgtatag gattaagttg gtctgtactt attaccaaac caattatttt cttcggcgat
    13441 atttcaaaaa gctccttggg cggctttact tccggcatca gcggtatatt tgccactttt
    13501 aaattcttgg ttgccaaaaa catacccaga ggggttttgg aagtcctgga aatacccagc
    13561 agcaccaggt ctgccttctt gatgcctctc ggatccttgc agtcatcata ttttaccgca
    13621 aattccacgg cttctatctt gctgaagtat tcatcatcaa gctttcttgc cgcgcccggg
    13681 atatcagtcg gatgctgtcc tgtaactttg gcgattgcct ctacagccgg ccccattatg
    13741 tcaacacaga taatattatg catagccgcc tgttcagtaa gatacttcct gagctcaggc
    13801 acaacaagag tgaacattat tatactgtcc aggtctttgg cctcactgat aacttcctgt
    13861 atgctctcta tctcggtaac atacggtatc catctgatcc tgatttcaca ttcgggatac
    13921 tgggcggatg cggcttttgc cacttgttcc gcagtctcac ctattgaatc tgatatcaca
    13981 aatattactg ccctgttttt attcataata tccccctaac ctattgctta tataaatcat
    14041 atagcagttt agtcatattg gttttggaca ccttgcctgt tattttgaac tgttctttgc
    14101 catcactgtc tttaaacggt tccactaccg gtatactgtc tatttcatgg gtgataagct
    14161 taactactgc atcatatgcg ctgtcattat cgaatatata gactatatta ggcatccttg
    14221 acataatcat tccaatcggc atattttgta tatcattatt accgattgca atctttatga
    14281 aatcctttct tgatactact ccggaaagtg ctcctttact ctgcacgtat attgtacctg
    14341 catctcttaa aaaaagcatg acaatagaat catatactgt tgctgattca tccatgacca
    14401 cagcacttga cttgatttct gcaaccttaa tgctttttat gaaatcatgg ccaatgtcgt
    14461 taattatctt gccggagtaa aaatagccaa ccttcggcct ggcctcaagc atatcggaca
    14521 ttgtcagtat tgtaagatca ggcctcaatg ccgcccttgt catattaagc tttgccgcca
    14581 gctgctcgct tgtgattggt tggtatttct ttaccaattc aattatctgc ttctgtctgc
    14641 ttgtaaattc tattgcaacc acctgcctca aggaaatatt cagagccatc ctgtctgggg
    14701 ctgtgtatgc actcctgttt attcagccca attttttact ttattctcat taaatactat
    14761 cataatgcat tgacaagcac aacaaaaatc aattatttat tcatcactac agcactcaaa
    14821 tcagcaattc tgttcatcat gtcccctatg ctctttaaaa gccccagcct gttgttcctg
    14881 attttctcat cttcaaccat taccataata ttgtcaaaga agtcgtcaat gggtttttta
    14941 agtgttatca taagctccag agcctttcca taatctctgt tgtctacgca gttcacaaat
    15001 tttgcataaa ctttatcgta gtcatgcaaa agtttcagtt cagccttctc agtaaacagc
    15061 aactcgttga tttccgtgct cttgccctta gatgccaggt tggatacccg attgaaggaa
    15121 cccagtatgt tcatgaactc atccttgtac ttccattttg aaagctccag aatcttcagg
    15181 tatgagtcat atatatcgtc aaacccggca tttataacag catcaataac gtcatactca
    15241 aagcccttgt ctatcatgac atttttgagt ctttgcctga agaattccaa tatgtccctc
    15301 tttactgact tttcatcacc cttcaatatc cctttttctt caaaaggctt aagggaatta
    15361 tgaattaatt gcgccaaacc aagatgaatg ttactgtcga gtattattga cgtaacccct
    15421 attgcctgcc ttcttaaggc atatggatcc tgagagcctg tcggttgaat tcctatggca
    15481 aagcacccgc atatggtgtc aattttgtct gaaatgccaa gaattgaacc attcaatgtt
    15541 tcgggagtac aatcccctgc aaacctcggc aggtaatgct caaatatggc atccgccact
    15601 tccctgtcct ctccttgcag cagtgcatac tcccttccca ttatcccctg aagctcgtca
    15661 aattccttaa ccatattggt gaccaaatca gctttgcata agtgtgcaga cctcagcaga
    15721 ttctttttcg caccggcgtt taatcccaaa atcgcagcca gatattctgc tatggcagaa
    15781 attctctgag tcttatcata tattgttccc aaggtttcct ggaataccac attcttaagc
    15841 ttttccgcgc aattatccaa ggattccttc ctgtcctcgc tgtagaagaa atccgcatca
    15901 gccagtcttg ccttaagaac cttctcattg ccttcccgca ccgtttcgag gaagcggctg
    15961 tcaccatttc ttacagttat gaagtaattc ataagattct tatcattatc cacaaccgga
    16021 aaatacctct gatgctcctt catgggagtt attactgctt ccttgggaag cttaaggtat
    16081 tcggcatcaa aaccgccaat aagtgcggta ggatactcta caagatatac aacctcctcc
    16141 agcagctctt catctataag cacttccccg cctttttccc tggccagctc ctcgcactgc
    16201 ctgcggatta ttgcgcgtct ttcctcctgg tccgctatta caaagcctgc tttcagctta
    16261 tcaaaatact ctgcagtgtt atttatctta atactgcctt tagataatac cctgtgccct
    16321 ttagtatacc ggctgcactt tattccgtcc ttgtcaaact ttatcagctc atcccccagt
    16381 attggcacca gccatctgac aggccttgcg aatctgaagg acttattgcc ccatttcatt
    16441 gacttgggaa aagatattga agtaagtata tcggggagta tggttttcag tactttttta
    16501 acaggctgcc ccttttcctg cttcttgtag tatacatatt ccacaccgga aagctcctgt
    16561 ataaaaacat caccaagctc tgccttccgg ccctttaaaa agcccagcag cgctttgctc
    16621 ggatttccgt tttcatcgta ggctgccctc ctggaaggcc ccttcaccag ttcctccaga
    16681 ttctcctgct gttccgcaat gcctttgaca aacagcacta ttctcctggg agtaccatag
    16741 gcctttatct cttcaaaagc tatcctgtaa tttttaagca ttgctccgga aatacttttg
    16801 acctgcttca gggttgaagg catataattt gcgggaattt cctcagtgcc tatttcgaat
    16861 aacaaatcct tactcatttt tttcacctcc gaatacagcc ttcttgagaa gaggatatcc
    16921 caattcctct ctctgcttca gataaccttg agctacaagc cttgccagat ttcttaccct
    16981 tccgataaag ccggtccttt cagtaacgct tatagccttt cttgcatcaa gaatgttaaa
    17041 agcatgtgaa catttcagaa cgtagtcata tgccggaagt ataaggccta tatccagcag
    17101 ccgttttgcc tctgcctcat attcgtcaaa aagtgtaaaa agcagtttgg tatcagcttc
    17161 ctcgaagcta tactttgaat gctcgaattc cgctgtcttg aatacatctc catagtttat
    17221 ccccgaaacc cattcaatat caaatacatt atttaaatcc tgaatataca tcgcaattcg
    17281 ctccaaaccg taggtcagtt ccgcagactc cagctgacag tctatgctgc ctacttgctg
    17341 gaagtacgtg tactgggtta tctccattcc atccagccag acctcccatc cgagtcccca
    17401 ggctccaagt gtgggagcct cccaattgtc ctcaacaaac cgtatatcat gcttgacggg
    17461 gtcaatcccc aatgctttca ggcttcccag gtacaaatcc tgcacgttgt cgggagaggg
    17521 cttcaatatt acctgaaatt gatgatgctg atacagtctg ttggggttct ctccatacct
    17581 tgcatccgca ggcctccttg aaggctctac gtaagcaatc ctccaaggct ccggccccaa
    17641 agccttgagg aaggtatatg ggctcattgt gccggctcct ttctccacat cataaggctg
    17701 tgcaataatg cagtgccggt cagcccagta tttttgaagc gtcaggatta tttcttgaaa
    17761 tgtcattttt catccctcca taataaaata gaaaaaatag aaaaaccctt catcccttga
    17821 agtaagggac gaaaggtctg cttccgcggt tccacccttt ttgacttgaa atcaatcaag
    17881 ccctcttttt taagcttatg ctccaaagcg cccgtaatca catgcactgc cggctcacac
    17941 catgtgcttc atccggtgaa gcacccccgg ttctctgata atgcacttcc tgattactcc
    18001 tcttcttcaa cacattttag cattaacact aatttagcaa atcataaata ttttgtcaat
    18061 aatcaatatg ttttacacta aaccttttac attgaattca actagtgtaa aacattttcc
    18121 ttgattcata tgttgtgaaa aatacattgc tttaacacaa aatcagaatc tatctgattt
    18181 tgtaatgata aatctttttc acaacatata ttattgctta aatgtattgt cggtattgtc
    18241 attgacagct ccgaaaatat ccgatgcatc cgccgccgca gaattatgtc ctgcttccga
    18301 tgatgtcatg tttctgaaat catctgcggc atttttgatt ttgcctgcca aattatctgc
    18361 tgtatttgtg attgcttcag atacattaat cgtctgcttg tttggccttt ctacttctat
    18421 tacgcatttt gaaagcagcg caactgcagt tcctattgcc gtaagctgcg gtatcaccac
    18481 tgcgcttatt gctcccgcag ttacaggaat gtcaagtatt acatagtcat ctttctttac
    18541 tcttattctg ttcacgttgc cggatttgac tattcccttc agcttttcaa cagcctcgct
    18601 gccttttacc gagatagtct ccgtccacct tttatctccc gcctcttcaa tatttatcaa
    18661 ggcatccact acattgccgt ttgcggcttc cagagcttcc ttggcttcct tataggtcac
    18721 tcctgttcta tccctgatga tatcaacctt ttccagactt atatccatgg aacttactcc
    18781 tttcagaaca ttaataaatt tacttctggg cattcaaatc cttcatagag tctaaaaaat
    18841 caagggactt aaacctttta tccaggtgac tttggatgaa ggatcttgtt attttttcaa
    18901 tttcattaaa aatagtatta tcaattttct tagtatttag tctagaaatt tcaatgttca
    18961 acaaaaaatt aatggcttca gccgtacctt ggcttatcct gaaaacttct ccggcatttt
    19021 tttgattttc accggtgctg cagctgccgc atattactcc gcccaaatca acactgaatc
    19081 ttgtataatc ctcactgctc tttctgcata tggcacacct ggcaatatta ggcctgtacc
    19141 cgctgatatc cataagcttg agctggtagc cgacacccag tatccctatt ggtatatttt
    19201 cggttgaaag atagtatagt gtttttgcaa gcagcaggaa cagtctttca ctgggaatac
    19261 cctcatcgga aacagcttct gcaagctcgg caaaatatgt ggcataagaa agcctaagca
    19321 aatccttcct tatgttataa aaggtttctc tcgttactgc ctgggaaatg tgataaaagt
    19381 tcgtgccctt atataatacg aactcactga aaacaaaaac ctcagacgat gaaagaaggc
    19441 tgcttttagg ttttctgcag cctttggcaa ttgctttgat ttttccgttg tttctcgtaa
    19501 gtatcgtaag tatcttatcg gcttctccca ggttggtata cctcaggact ataccttccg
    19561 tcttggatat cggcattaaa tcactcccaa ttatatccga agttcttaag tgcacttctg
    19621 ctgtcacgcc aatttcttct caccttgacc caaagctgaa gattgatcct gcaatgcagc
    19681 aggttctcta tgtcaactct ggatctgaca cctatctctt tcagcatcct gccttgcttg
    19741 cctattatta ttgctttgtg tgattccttc tcacagtata ttgtagcagc aatatccatg
    19801 agatccttat tttctctttc tttcatggag gtaatttcca cggcagtgcc atgaggcact
    19861 tcctctgatg taagctcaag aaccttctct ctgataattt ctgatacaat gaacttctca
    19921 ggcatatcgg tatatatatc ctccggaaaa tacctgggcc cttccggcag atgcttaact
    19981 atttccttca gaagctcatc cagattctcc ccggtgcttg ccgaaacagg tattatactg
    20041 tcaaaataat cttcatgcat tattaaggaa gctgcaatct gttccagcct ggatttgggt
    20101 atcttgtcaa ttttgttcag cacaagtata accggggagt taactttttc cgccacctgt
    20161 gcggcataca tgtctcccgg ccctacttca gctgaacagt caaacatgac tacaactaca
    20221 tccacgcctt tcaaggactc ggttgccgtt ttcaccatat actcccccaa catgtgcctt
    20281 ggcttatgta taccgggagt atcgataaaa actatctggt actcacttgt ggtatatatg
    20341 cagcggattg agttccttgt agtctggggt ttgctggaaa taatggatac ccgctccccc
    20401 agcagcttgt ttataagagt tgatttgccc acattcggcc ttccgatgat tgtcacaaat
    20461 cctgatttaa attttgtgtt cataatacta ctccttatat gaagaatgcg acgcattctt
    20521 cgggtacgag gtacgaggcg cgaggttcga g
//
LOCUS       DAOQUR010000040        19996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3156_length_19996_cov_12.250489, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000040 DAOQUR010000000
VERSION     DAOQUR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3156_length_19996_cov_12.250489"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKI50_08820"
     CDS             complement(<1..878)
                     /locus_tag="PKI50_08820"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNT03841.1"
                     /translation="MVLRNALDLISNTPVVALDNITGNIEGRIFAKLEFWAPGLSKKD
                     RVALQVIEDAEKSGRLKPGQPVVELTSGNMGTGLAIVCKLKGYKFIAVISKGNSIERA
                     KMMKAFGAEVVLVDQMPDSIPGQVSGKDLELVEIEAQRLTKELNAFRVDQFYNPSTAR
                     AGEVGVANEIIQQIDERIDAFVDFMGTGGSFIGVTTTLKKHFPDIKCFGVEPENARRY
                     SNPQDIGSGKHKIQGGGYNMELPLMTGDKLKLVDGFITVSDEEAIQAARRLACEECIF
                     AGFSAGANLAAALKLL"
     gene            1069..1584
                     /locus_tag="PKI50_08825"
     CDS             1069..1584
                     /locus_tag="PKI50_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03842.1"
                     /translation="MKKLLLFALILLLSALMIGCTAAGSQSRENEEKTQQTDEADKEA
                     AANIVEGFGKKLQEVSLLAPKEVLEKSMEESYGDFVTSELIAKWAKDPMNAPGRLTSS
                     PWPDRIEILAIEKSAENEYEVKGEIIEITSTEKAEGGISAKRPITLAVRKIEDRWLID
                     DVKLGDYEKIK"
     gene            2115..2618
                     /locus_tag="PKI50_08830"
     CDS             2115..2618
                     /locus_tag="PKI50_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT03843.1"
                     /translation="MAVREILLLGNENLYNVSKEVSKEEIDKAKQIVDDLHDTMADFR
                     KKYGFGRAIAAPQINEFFRIIYMNFDDREIALINPRLEFTDDEKFEMWDDCMSFPGLE
                     VRLSRYKKCRVYYKDLEWKDCIFEPEGDISELIQHEYDHLDGILAVQRAIDNKSYRIN
                     KNKAGCY"
     gene            complement(2621..3433)
                     /locus_tag="PKI50_08835"
     CDS             complement(2621..3433)
                     /locus_tag="PKI50_08835"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HNT03844.1"
                     /translation="MNILVTLNSNYITPLKVMLKSLFFNNWRERFSIYIMQSSLTDRE
                     LQQIEDIVINQGHEFHVISIKDEYFKDAPVVMHYSKEMYYRLLAFKFMPPKIDKLLYL
                     DPDILIINGIRELYNTDLTGYLYAAAYHHRGSINKINKIRLKPYDIEAYYNSGVLLFN
                     LNQQRNTIDEKDIFDFVRENADTLILPDQDILNALYSKSIKPVDEILYNYDPRYYNYY
                     KIISNGKYDMNYIIYNTSIIHFCGKQKPWKKNYSGKFHSLYKHYERIALEQS"
     gene            3597..5132
                     /gene="cls"
                     /locus_tag="PKI50_08840"
     CDS             3597..5132
                     /gene="cls"
                     /locus_tag="PKI50_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNT03845.1"
                     /translation="MQKLLRMIFSRVFLVGTAIFIQVFALLIMIWRFSNYFVYFYTFS
                     TLLSIVVVLCIVNGKSNHAYKLAWIIPIMAFPIFGGLFYLMFGGNKSSKRNKRKMQVI
                     TDKMQNVLVQNLKVMQNLEKEDRGAANQSKYIENYSSCPVHTNTRTEYLTPGERKFER
                     MLEELKKAERYIFLEYFIIDKGVMWDTILDILKEKAMQGLDVRVIYDDVGCILTLPYG
                     YDKELERMGIKCCVFNPFVPVLSIRLNNRDHRKIAVIDGRVAFTGGINLGDEYINAYE
                     KYGHWKDASIAIEGDAVWSLTVMFLSMWDFLRKEDEDYEKFRPTYEYTKDPSYNGYVQ
                     PFADSPLDEEAVGETIYLNLIGEADRYIYINTPYLILDNEMNSALCMAAKRGVDVRIT
                     TPYIPDKWYVHAVSQCNYEALIESGVSIYEYTPGFIHAKTFVVDDLYAVVGTINLDYR
                     SLYLHFECGVWMYKTNSILEVKADFLKTLKVCQRITLGCCRDEVLYKRLGRTILRIFA
                     PLM"
     gene            5165..5590
                     /locus_tag="PKI50_08845"
     CDS             5165..5590
                     /locus_tag="PKI50_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HNT03846.1"
                     /translation="MRIAVIDGQGGGIGKAIVERIRQKLEDKAEIVALGTNSLATSLM
                     LKAGANEGATGENAIIHNACKVDIILGPIGIISANSLLGELTPLMAKAIAESPAKKIL
                     IPMSKCNIAVAGIEIKPIPSYIEDAVEIVKTMIKDTKVV"
     gene            complement(5605..6066)
                     /locus_tag="PKI50_08850"
     CDS             complement(5605..6066)
                     /locus_tag="PKI50_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNT03847.1"
                     /translation="MSLESVRKFFDENGYSDLQIIEKDASTATVELAAKAHGVAPALI
                     AKTMAFGLKDRNILIVVSGDSRIDNRKFKDCFHEKASMLNPEKVLEATGHAVGGVCPF
                     GLKNPMDIYVDISMKEFEAVYPAAGSANSAVKITPDRLQEVTGGTWIDVCK"
     gene            6549..8135
                     /locus_tag="PKI50_08855"
     CDS             6549..8135
                     /locus_tag="PKI50_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT03848.1"
                     /translation="MKNLKFEELNLSDEILKAIGNKGYEEATPIQAQSIPYIMEGRDI
                     IGQAQTGTGKTAAFGIPILEKFNQGEKTVQGIVLCPTRELAIQVAEEIRELAKFKRGV
                     EVLPIYGGQPIDRQIKALKRKPQIIVGTPGRVMDHMNRGTLKIDKVRMVVLDEADEML
                     DMGFIDDIVTILKVVPEERQTMLFSATMPKPILELTKRFQKDPKHVMVVHKELTVPKV
                     EQVYFEVKEKTKLEVLSRLIDMYNPKLSLVFCNTKRKVDELVAELQGRGYFADGLHGD
                     MKQVQRDRVMSKFRSGAVDVLVATDVAARGIDVDDIEAVFNYDLPQDEEDYVHRIGRT
                     ARAGRGGRAFTFVVGREIFKLRDIQRYAKAKITLQKAPSSEDVEEVRTNLFLDKVIEI
                     MDEDDLSQYVSIIERLAGEDYTSIEIAAALLKMAVGNKDKEVQHEETDFNDTGAEDGM
                     VRLFINIGRNQKIRPGDIVGGIAGETGIPGKLIGSIDIYDKYTFVEVPREYAGDVIRI
                     MKDNQIKGRRINIEPANAKH"
     gene            8426..9694
                     /locus_tag="PKI50_08860"
     CDS             8426..9694
                     /locus_tag="PKI50_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT03849.1"
                     /translation="MSLIKDLISPYKGMPREIYIIFISRIVNAMGCFVMPLMTIIMTD
                     RIGLSNEAAGFFLSLNSMVFPVASILGGKLADTFGRKHLIIIFGTLGALLYMSCGFIK
                     PSMQLIYVILGASTLMFVAGPAHDSLIADLTTPENRAKAYSLSYLGWNMGFAVGPIFG
                     GLLYRRYLPLVFIGDAVTALIATGLIYFFINETIESTKNDITDESRKLEKREKGSIIS
                     VLLRRPILIYFSIIALGYEFSYSQWNFLMPLHSVKNFGEMGAQYFGWIASFNGLVVML
                     FTPILTRLTRKTRGIRIMVHGILLYAIGFGMLGLIYKLQYFFVSALIFTLGEVLLTIS
                     VMPFIVNHTPASHRGRMSAVLTTITGTGYAVGPMAMGRILNFINIEKAWIFIGIFTAM
                     TAVLMFGLEKYEDRENTMREKEGSAVKVQQ"
     gene            9803..10669
                     /locus_tag="PKI50_08865"
     CDS             9803..10669
                     /locus_tag="PKI50_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNT03850.1"
                     /translation="MDTVLLIDSCSDLPRSYVDENAIPFVSLVCNFKDKEYKDDFGKS
                     ISYKEFFDAVRSGEMPSTAQVNVYEYTEIFKKYATEGKSVIYLGFSSALSGSLGSAYL
                     ARDMVMEEIKDADITIVDSKSASLGEGLLAYNANEMLKAGASKDEVVSWLETNKLKVN
                     HWFTVDDLGHLRRGGRLSGTAAFLGTLLDIKPVLKVDDEGKLIPISKNKGRKKSIKTL
                     FEMLQENIEAPEEQVIAISHGDCIEEAEYLKEMILKEYNVKDVIINHVGPVIGAHTGP
                     GVVALFFFGNKR"
     gene            11052..13523
                     /locus_tag="PKI50_08870"
     CDS             11052..13523
                     /locus_tag="PKI50_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT03851.1"
                     /translation="MKKRRTLKRERINRELTNIFKYPLTIVEAPMGYGKTTAVSEFLA
                     LKGVPVIWTSFLSEEDTAHGFWERLAAEIGKFDKAAESRLKSLGVPADTPQTTMAVSI
                     INEMGYKPDTTLVIDDFHLARSMRVTALFRRFVMEMPDDFHIVILTRDTTNLDITELS
                     AKGLCYIVSQNMLRFTDSEIRDYCALMGFTASEGEIRKIGDYTGGWISLIYLIMLGME
                     RGIPVGRNGTINELVEKVLYNAYDERIQRFLLCLSVMDSFTAEQAVFVTQEEKSEETL
                     KKLRRENAFIAFDEAAGVYIIHNVLLDFLRGKQKDEEERAALYRRLGEWYLAQRKYRT
                     AYGYLYRAGESERILALLDNGDTITSDSATFDGALDLFSAMPREMLHKYPLAYLQYIA
                     IILTNGDPDDFQDGVIRLNELREACERAEDPVPHGRDRVLAELYLTQIFAVFNDAKEM
                     AACIEKAQRLLDGDVSFLQKREAEFTLGSPHLLYTCYREPGSLKELTELMASDFPIFA
                     ELADGCSTGCDYVVRAEYALETGNWQAAELDAFKGIYKAQTKEQIGLVICAYFVLIRL
                     YLYKGKTNEALELLRQLRQDVKRTNNLYYNTGLEIVEGYVYGCLTRLNSIPLWLQTGD
                     MSPAQFFYEGLGFKYIVYGKALLLSKNYIKLEMLTEEFARCFAVFHNQLGFLHNQIFR
                     ATAKYPLYGMEVGCAALREAFSMAREDHIILPFAEYAPAVIDMVRHIAHADSRDAYIK
                     KVLEACVHYMESLKHSIQSAVSLSARELEILTLAAEGLKRDEIAGKLHVTAGTVQTHL
                     HNIYLKLEVSGRMAAIKKAQKLKLL"
     gene            13646..13903
                     /locus_tag="PKI50_08875"
     CDS             13646..13903
                     /locus_tag="PKI50_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HNT03852.1"
                     /translation="MSKILKVTPNEDYTLLVEFEHGNKIIFNMRDIIKTIPFSSLEDL
                     NRFKNVTVEEKAISWPEPVPGEKSIMPLRLTVDNILFAIRD"
     gene            14168..19942
                     /locus_tag="PKI50_08880"
     CDS             14168..19942
                     /locus_tag="PKI50_08880"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNT03853.1"
                     /translation="MTRAMYVTVLGRVAGVDTSQYNTSAFKDVAVGAWYAPYVAWAAE
                     NGITHGTDNDTFSPDEAITREQMATMTVRYFKSCNISYEAERQIASGPKDAPAISSWA
                     LDSVLSLWHAGLLAGDENGNFNPASNATRAEAAAFCMRISKTVKQQEEKKQPTPAPAP
                     TPASSQGSGNDRDESRSDTKYTITFVTNGGDAIDKLRLKPGTALDNLPVPYKMDAVFL
                     GWYKDSSLSTPVSSTDIITGNMTLYAKYAASTGFTEELSVPTISKLDQDKTFTIIVVD
                     STDGMTAAEVKAGMTFDSPSNPGFAGIQVDESSDGEFIVSAIGGAFDEGGTFKLTLDN
                     DNLSFKGEDESTRICTFTIAKSPVMNLSLNKNMKYLSAGQISDLTQNGFSVKSLSIPL
                     VRVAGDGEGLSGIDAEGGTFVYDGGGINVGDTVAIYEGIRPDQRDLDTSGDDAGDIAY
                     VTITAISGTTYSYVKADEKDILFTPDVLPVSTTADTDGSSDNNSVTVPQSVMAYTDAI
                     YADIGLNAATTVDVGDFIAFHTGTFGSADAENAGYAEITSVTVSGDNYIIAYISTTPE
                     QMWEATDLYTTKEVDGDELLENVDIAALEESVERQAIESGFADEAAQYLAEMAMKTDS
                     FRQLNLTAMADTSSYGAMSMLEAFGTGPEVKNLKVNARVFNYLQHLGGSGARVTLTIS
                     CDIVFQAAGEDNNIVIRLSGAFEQEVKVDLNVSCETKWTLLDGWFWWAEDTLVTASID
                     TGTYTSININASIGTFEKGAQCDLSKFQDIGKQIKDIMNGKKPEDSAIADTLREKYKA
                     MLEDDSDWVDIYKKEIFTFEQAIDPFHIVVFGMDVDFVVGAKMNVSIGCNFSYESEKR
                     YIFSLYINAQTATSDTIDLRPEQYNFTFYIMGTLGLRAGISIEIKVGLFSLDLASIGI
                     EGEVGAYVQLWGYFYYEASYRQATGLTSKYGGAMYLEFGIYLEISFVAQAIDNMFEYE
                     ATLYENQWPLLTFGSQEDVYDFEYSADKAPTAFMAKQTKSFVLPDSVFSMNYMDMKTG
                     DIESKNYDPSNFDYSTTNSDFTYDPATKTVTVAPGEKDITEGALVLIWKGNPVAFTSV
                     PIARAVPLHWEDLKSAYTISFNSRGGSAVDTIALPYKGVITPPANPAKAGYVFGGWYK
                     DEALTQSYTFPATMPAEDTVLYARWVPAPNTKYRVEHYKQNLENDLYTLAETEYRTGT
                     TDSVVHVSPKSYEGFSLVPSPEIVQILPDGSTVHVFHYNRNSYSATFQPNNGKIGADV
                     ENTVKFGGKINIPVFTRARHSFAGWSPAVPETMPAQNMTFEAQWTTVGQVAYKVEHYK
                     EGLDGSYALAETENLTDVANQMVTATAKSYGGFSYNRNAPGTVPSGIVTSDGNLVLKL
                     YYTRNSYTLTFDANGGTGGITRQAKFGEPISAPAVAREGYSLTGWSPAVPATMPAENA
                     TYTAQWQAVGLIPYRVEHYLENLDDGYILTDIENLSGAEGDTATASTKSYNGFEYLAD
                     PPTVASGTIAADGSLVLKLYYMRKDVTLEFLADGGSAGDNELDYQYGDFFNEFPPGTI
                     EGYAFNGWSPAFMGTAPAEDTTYTAQWTSNISEALDVTTAVYANQDYTAGGVYWDYEA
                     QTLTLTDVVITSPDSAESGVDNFALKVPDGTTIILVGSNYLQSGKGPGINSYGILCDG
                     DLIIQGPGTLTALGTPNFMSLADQIVCYGIYAAGTITIEDDAAVIAKSTGATGNSCGI
                     FAEDVVITDSTVLASSNESANGIRCGIYASGDIDITNSTVNTPSGIYASGDAAISEST
                     VNALNSGIEYPDDVFNYYGIYGDNVAITGGTVYVTSQSADGYGIYAAAGVEITGSAAV
                     TARCSDFGGPGTGSGIYAGTGSISISGSTVIAAGKDALAMNKAPVLNNAEAFCASTSA
                     DGSGGVIYDSGDIATYKYIQIRAD"
BASE COUNT         5803 a         4033 c         4906 g         5254 t
ORIGIN      
        1 ttaagcagct ttagtgccgc tgccagattc gccccggctg agaagcctgc aaaaatgcat
       61 tcctcacatg ccagccttct ggctgcctga attgcttctt catctgacac ggttatgaaa
      121 ccgtctacca gcttcagctt atccccggtc atcaggggca gctccatatt atagcctccc
      181 ccttgtatct tatgctttcc gcttccgata tcctgaggat tgctgtagcg ccttgcattt
      241 tcgggttcaa ccccaaaaca ctttatatcg gggaagtgtt tttttaatgt tgtggtaaca
      301 ccgataaagc tgcctccggt acccatgaaa tcaacgaaag catcgattct ttcatctatt
      361 tgctgaatta tctcattggc aaccccgact tcccccgcac gagcggtgct gggattataa
      421 aactggtcta ccctgaaagc attcagctct tttgtaagcc tttgtgcttc gatttccacc
      481 agttccaggt cctttccgga tacctgccca ggaattgagt ccggcatctg atccacaagg
      541 acaacttcag caccgaaggc tttcatcatt ttagcccttt ctatagaatt gccctttgaa
      601 atcacggcta taaatttata gcctttcagc ttgcagacga tagccaatcc ggtgcccata
      661 tttccgctgg tcagttcaac aactggctgg cccggtttca gccttccgct tttctccgca
      721 tcctcaatca cctgcaatgc aaccctgtct ttcttgctta aaccgggagc ccagaactcc
      781 agctttgcaa aaattcttcc ctcaatattc cctgttatgt tgtcaagggc tacaaccggc
      841 gtgttgctga tcaggtctaa tgcattcctg agtaccatat aggaacacct ctttcattga
      901 ttattttgtc atatagtgta ttatgctgca tattatagca ttgggcatta ctattcacaa
      961 ggggcggatt gaggaatggc atattaaggc ttaaagaagt gcttgattga tggaacgaaa
     1021 agggtgctta ttacgtctat taatcaaaga ctatttggga ggtatcatat gaaaaaacta
     1081 ttgttgtttg ctttaatact gttgctgagc gctttgatga tcggctgtac ggcagcagga
     1141 agtcagtccc gggagaatga ggaaaagacg cagcaaactg atgaagccga taaggaagca
     1201 gcagcaaata ttgttgaggg ctttgggaaa aagcttcagg aggtctcgct tctggctcca
     1261 aaggaagtat tggaaaagag catggaagag agttatggcg attttgtgac ttcagagctt
     1321 attgcaaaat gggcaaagga tcccatgaat gctccgggaa ggctgacttc gagcccctgg
     1381 cccgatagaa tagagatcct ggctattgag aaatcggcag aaaatgagta tgaggtaaaa
     1441 ggtgaaataa tcgagataac cagcactgaa aaggcagagg gcggaatatc ggcaaagcgg
     1501 ccgatcactc ttgcagtcag gaaaattgaa gaccgttggc tcattgacga tgtaaagctt
     1561 ggcgattatg agaaaataaa ataaaaggaa cataacgatc taccttgcac caatgcagac
     1621 agcttattac ccagccatga tctttcgacc atgaccaagc agttcaccac ctgcaatgat
     1681 ggttcgttcg accatgcaaa gtacctgcac agccacatac aacctcccgg ctgtatatga
     1741 ccattacggc accctgcata atcttagctc gaccctgtat gataagttgg gatcctacta
     1801 taatcaaggg tagtcgacta taacagaacc tttccttacc atacaggatc ttccctccga
     1861 ctgcacacag gcctttcgac ccatatacaa tctttggccg acccatacaa gcccccgcta
     1921 aggagcatat atgagtcttg gctcaaccgt tatgttccta cgatattaat ttgtccggaa
     1981 cagaaaaaaa taatactaag ataataaagg ttcttttgcc aggacttgat taaaactgta
     2041 taatatatat aaataggcga aaagatgacc tttaaaaact ttgcgtcctg ccgcagcatc
     2101 aaggagggaa gattatggcc gtcagagaga tattgctgct ggggaatgaa aatctataca
     2161 atgtatccaa agaggtttca aaggaagaaa tagataaagc aaaacaaatt gttgacgatt
     2221 tgcatgacac aatggcagat ttcagaaaaa aatacggttt tggaagagct atagctgcgc
     2281 ctcagattaa tgagtttttt cggataatct acatgaattt tgatgacaga gaaatagcat
     2341 taataaatcc aaggcttgag tttactgacg atgaaaaatt tgaaatgtgg gatgactgta
     2401 tgagttttcc cggattggaa gtaagattat caagatataa aaagtgcaga gtctattata
     2461 aggatctgga gtggaaggat tgcatttttg aacctgaagg ggatatatca gaattgattc
     2521 agcatgaata cgatcatctt gatggtatat tggcagttca gagagcaata gacaataagt
     2581 cttataggat taataaaaat aaagccggct gttattgata tcaagactgc tctaatgcta
     2641 ttctctcata atgcttatac agtgagtgga atttgccgga ataattcttc ttccatggtt
     2701 tttgctttcc gcagaagtga attatagatg tgttgtaaat aatataattc atatcatact
     2761 tgccattgga tataatttta taataattgt aatatctggg gtcgtaatta tatagaattt
     2821 catcaaccgg ttttatgctt ttggagtaaa gggcattaag tatatcctga tccgggagta
     2881 ttagtgtatc ggcattttct ctaacaaaat caaagatatc cttttcatct attgtgttac
     2941 gctgctgatt caggttaaaa agaagcactc ccgagttgta gtaagcttca atatcataag
     3001 gttttagtct tattttgtta attttgttga tagaccccct atgatgatat gcagccgcat
     3061 aaaggtaacc ggtcagatca gtattataaa gctctctaat gccattaata atcaatatat
     3121 caggatccag atagagaagc ttgtctattt tcggaggcat aaacttaaat gccaaaagcc
     3181 tataatacat ttctttgctg taatgcatga ctaccggtgc atccttaaaa tactcatctt
     3241 ttatcgatat cacatgaaat tcatgtcctt ggttaattac aatatcttct atttgctgca
     3301 gttctctatc tgttaagctg gattgcataa tataaatgct aaagcgttct ctccaattat
     3361 tgaagaaaag ggattttagc atcaccttca gaggagttat ataatttgaa tttaatgtta
     3421 caaggatgtt catgatcagt ctccttagat aatatagttg tttctatgtt taaatataag
     3481 ttatgcagca aattaagtca attatagtaa tatcaatata tattttgcta tgttccgatt
     3541 agaactgtat aataaacata agggtatgga taaaacggag tccgatggag gttattatgc
     3601 aaaagctgtt gagaatgata tttagcaggg tttttttggt tgggactgcc atatttattc
     3661 aggtttttgc cttgttaata atgatatgga ggttcagcaa ttattttgta tatttctata
     3721 ccttcagtac attacttagt atagttgttg tgctgtgtat tgttaatggg aagagtaatc
     3781 atgcatataa gcttgcttgg attattccta tcatggcttt tccgattttc ggcggacttt
     3841 tttatttgat gtttggcgga aataagtcaa gcaaaaggaa taaacgaaaa atgcaggtaa
     3901 ttactgataa gatgcaaaat gtcctcgttc agaatttgaa agtcatgcaa aatcttgaga
     3961 aggaagacag aggcgccgcc aatcagtcaa aatatataga gaattattct tcctgtcctg
     4021 ttcatacaaa tacaagaaca gaatatctta cacccggtga gagaaaattt gaacgcatgt
     4081 tggaggaatt aaaaaaagcc gagcgttata tttttcttga gtattttatt attgataagg
     4141 gcgttatgtg ggataccata ttggatattt tgaaagaaaa ggcaatgcag ggcttggatg
     4201 ttcgtgtcat ttatgatgat gtgggctgta tattgacatt gccttatgga tatgataaag
     4261 aactggaacg gatgggcatt aaatgttgtg tatttaatcc ctttgtgccg gttttgtcta
     4321 tccgcctcaa taaccgtgat catcgtaaaa ttgccgtaat tgatggtcgt gtagcgttta
     4381 cagggggtat caaccttgga gacgaatata tcaatgctta tgaaaagtat ggccattgga
     4441 aggatgcgtc tattgccatt gaaggggatg cagtatggag ccttactgta atgttccttt
     4501 ccatgtggga ttttctccga aaagaagatg aggactacga gaagtttaga ccgacttacg
     4561 aatatactaa agatcctagc tataatggtt atgtacaacc ttttgcggat agtcctcttg
     4621 atgaggaagc cgtaggtgaa acaatatacc ttaatttgat aggtgaagct gacagatata
     4681 tctatatcaa tacgccatac ctgatacttg acaatgagat gaattcagct ctgtgtatgg
     4741 cagcaaaaag aggtgtagat gtccgcatca caacccctta cattccggat aaatggtatg
     4801 ttcatgcagt atcccaatgc aattatgaag cattgataga aagcggagtt tctatatatg
     4861 agtacacacc cggttttatc catgcaaaaa cctttgtagt ggatgatctg tatgctgtag
     4921 ttggtacaat aaacttagat tatcgcagtt tgtatcttca ctttgagtgc ggtgtctgga
     4981 tgtataaaac aaatagtatc ttagaagtta aagcggattt tctaaaaact ctaaaggttt
     5041 gccaaaggat tactttagga tgctgtagag acgaagtgtt gtataaaagg ttgggcagaa
     5101 cgatcctcag aatatttgca cccttgatgt aaaggattta tttatgactt attggaggga
     5161 taatatgcga attgcagtaa tcgatggtca gggtggagga attggaaaag ctatagttga
     5221 aagaatcagg cagaaacttg aagataaagc agaaatagtg gctttgggaa ctaactctct
     5281 ggcaacttca ttgatgctga aagcaggcgc taatgaaggt gcaacaggag aaaatgccat
     5341 tattcataac gcctgcaaag tggatataat tttgggcccc ataggaataa taagcgcaaa
     5401 ttcactgtta ggtgagttga caccgcttat ggcaaaggct atagcggaga gtccggcaaa
     5461 aaagatactt attcctatga gtaaatgtaa tattgctgta gccggtatag aaataaaacc
     5521 gattccttct tatatagaag atgccgtaga aatagtgaaa actatgatta aagatacaaa
     5581 ggtagtgtag ttgggataga ttctctattt acatacatca atccatgtac cccctgtgac
     5641 ttcctgtaag cggtcagggg ttatttttac agctgaattt gcagatcctg ctgcgggata
     5701 tacagcttca aattccttca ttgaaatatc cacgtaaata tccatgggat tcttgaggcc
     5761 gaaggggcat actcctccca ctgcatgacc tgttgcttcc agcacttttt ccgggttaag
     5821 cattgaggct ttttcatgaa agcagtcttt aaacttgcga ttatctattc ttgagtcacc
     5881 gcttacaact ataagaatat ttctgtcttt caatccaaag gccattgttt ttgcaataag
     5941 cgcaggtgca acaccgtgtg cttttgccgc cagctcgaca gttgcagtgc ttgcatcttt
     6001 ttcaataatc tgcaggtccg aataaccatt ttcatcaaag aattttctta cactttccag
     6061 actcatcaca ttacttcctt tcttttgtat aaattctcaa gacggcagta tatgaccata
     6121 cacattatat catagattga caaaaatttg agatatcaat catggatttt gcatttgcat
     6181 aatataagaa gaatgcgacg cattcttcgg gtacgaggta cgaggcgcga ggttcgaggt
     6241 ttagggcagg gcttcggagt tatgataaca ctgaagacac gaaaaaccgc taaatgcacg
     6301 gcgggataaa tattacgtta cataaataat tatcgataat aatataagta tcgaaatttt
     6361 cttatgcaac tatataatat ataatagtta ccgtgcaatc gcgcagcgat ttcgtgaaac
     6421 agccgtgtct cccgtgttac ccgttaccta tggcgcatta ttcttctaat atgaaaaaaa
     6481 aagttgcaat ttgagcataa taaatgtatt gttatatagt tacattaaaa aagattggaa
     6541 ggtagtaaat gaaaaatctg aagtttgaag aattaaattt atccgatgag attttaaagg
     6601 caataggaaa taaaggttat gaagaggcaa ctccgataca agcccaatct attccttaca
     6661 taatggaagg aagggacata ataggccagg ctcagacagg aaccggcaag acagctgctt
     6721 tcggtatacc gattctggaa aagtttaacc agggagaaaa gacagttcaa gggatagtgt
     6781 tatgcccaac cagagagctt gccatacagg tcgctgaaga aatcagggaa ttggccaagt
     6841 tcaaaagggg agtagaggta cttcccatat acggcggtca gcctattgac aggcagataa
     6901 aggcattgaa aagaaaaccg caaatcattg tagggacgcc cggaagggtt atggatcaca
     6961 tgaacagagg gacactgaaa atagacaagg tcaggatggt tgtgctggat gaggcggatg
     7021 aaatgctgga tatgggattt atagatgata tagttaccat attaaaggtt gtcccggagg
     7081 aacggcagac gatgcttttc tcggcaacta tgcccaagcc gattctggag cttactaaaa
     7141 ggtttcagaa ggatcctaag catgtaatgg tggtgcataa ggaactgaca gtacccaagg
     7201 ttgagcaggt atattttgag gtgaaggaaa agacaaaact cgaagtgctg tcaagactta
     7261 tagacatgta taatccaaag ctttcgttgg ttttctgcaa tactaaaaga aaggtagacg
     7321 agctggtggc agagctgcag ggcagggggt attttgcgga cggcctccat ggagatatga
     7381 agcaggttca gagagacagg gtgatgtcaa aattcaggag cggagctgta gacgtactgg
     7441 tagctactga cgttgctgcc agaggtatag atgtggatga tatagaagca gtattcaact
     7501 atgatctccc ccaggatgaa gaggattatg tacacagaat aggacgaaca gccagagccg
     7561 gcaggggagg aagggctttt acctttgttg tgggcagaga gatatttaaa ttaagagata
     7621 ttcagaggta tgcaaaggca aagattaccc tgcagaaggc accttcctca gaagatgtgg
     7681 aagaggtaag gacaaattta tttttggata aagtaataga aatcatggat gaagatgatc
     7741 tcagccagta tgtcagtatt atagaaaggc tggcaggaga agactatacc agtattgaaa
     7801 tcgccgccgc acttctaaag atggctgtcg ggaataagga taaggaagtg cagcatgagg
     7861 aaactgattt taatgacacc ggagcggagg acggaatggt aaggctgttc atcaacatcg
     7921 gcagaaatca gaagataagg ccgggggata ttgtcggagg tattgcaggg gaaacaggca
     7981 ttcccggaaa gctaatagga agcattgaca tatatgacaa atacaccttt gttgaagtgc
     8041 cgagagaata tgccggcgat gtaataagaa taatgaagga taaccagata aaaggcagga
     8101 gaatcaatat tgagcctgcc aatgcgaaac attagcatat tattaatatt gtattacaat
     8161 tttgcttgct gattgacagt aatgaaagga ctggagtata cttacaaggg taaaggaaaa
     8221 aatggaaaag gaggttgcga atatgagaat tagtataatt gcagacataa aaaataatat
     8281 agtgtcgcag cctgctttga gagtttttta gggttgatgg atatataact gcgggctgct
     8341 ctaaggagga gacaccgtga aatttttggc catggaatgt gtttgttcca tggcttttat
     8401 ttttttatga gaggggaagt acaatatgtc gttgataaag gatttaatca gtccctataa
     8461 gggtatgccg agggaaatat acataatatt catatcccgt atagtgaatg ccatgggctg
     8521 ctttgtaatg ccgcttatga caataataat gaccgacagg ataggcttgt caaacgaggc
     8581 tgcgggcttt ttcctgagcc tgaacagcat ggtttttccc gtggcttcaa tccttggggg
     8641 aaagcttgcc gatacctttg ggaggaagca tcttataata atatttggaa cccttggggc
     8701 tttgttgtat atgtcctgtg gttttattaa accctccatg cagcttatat atgtcatcct
     8761 gggggcaagt acgcttatgt ttgtagccgg acccgcacat gattccctga tagccgacct
     8821 taccacgccg gaaaacaggg caaaggctta ttcattaagc tatcttggct ggaacatggg
     8881 ctttgctgtc ggcccaatat tcggaggttt gctttacagg agatacctgc cccttgtatt
     8941 cataggggat gcagtaactg cgcttatagc aacaggcctt atttactttt ttatcaatga
     9001 gaccatagaa agcacaaaga atgatataac ggatgaaagc aggaagctcg aaaaaaggga
     9061 aaagggctca attatttcag tgctgctcag aaggcctatt cttatttatt tttcaattat
     9121 tgcattagga tatgaatttt catattccca gtggaatttt ttgatgccgc tgcattccgt
     9181 aaagaacttc ggtgaaatgg gtgctcagta ttttggatgg atagcgagct tcaacggctt
     9241 ggtggttatg ttatttactc cgattctgac caggctcaca agaaaaacaa ggggcatcag
     9301 aatcatggtc catggcatac tgctttatgc cataggcttc ggaatgctgg ggctaatata
     9361 caagctacag tatttctttg tctccgctct gattttcact ttgggagagg tgctattgac
     9421 aataagtgtc atgcctttta tcgtgaatca tacaccggca tcgcacaggg gaaggatgag
     9481 tgcggtattg actaccataa cgggtacggg ctatgcggtt ggccccatgg ctatgggcag
     9541 gattctgaat tttattaaca tagaaaaagc atggatattt attgggatat ttacggccat
     9601 gactgctgtt ttgatgtttg gacttgaaaa gtatgaggac agggagaaca caatgcggga
     9661 gaaagaagga agtgccgtga aagtgcagca gtgacggcaa taatttgatc catttgccct
     9721 gaaggctgat tgtcttattg atttatacct gaaaaatggt tactatagta gtaatataat
     9781 attgcagaga ggtgagaaca tgatggatac ggtgcttttg attgattcct gctcagacct
     9841 accgcgcagc tatgtggatg aaaatgcgat accttttgtc agtctcgtat gcaactttaa
     9901 agataaggaa tataaggacg attttggcaa gtctatcagc tataaagaat tttttgatgc
     9961 agtgagaagc ggtgaaatgc cttcaacggc acaggttaac gtttatgaat atactgagat
    10021 tttcaagaag tatgcaacag aaggtaaatc agttatatat ctgggttttt cttctgcttt
    10081 gagcggaagt ctcggcagcg catatcttgc aagggatatg gttatggaag aaatcaagga
    10141 tgctgatatt acaatagttg acagtaaaag tgcctccctc ggggaagggc tcctggccta
    10201 caatgcaaat gagatgctta aggcaggagc ttcaaaagat gaagtggtaa gctggcttga
    10261 gacaaacaag ctcaaggtga accattggtt tacggtagat gatttggggc atctgagaag
    10321 aggaggacgg ttatccggaa cagctgcttt tctgggcacg ctcctcgata tcaagcctgt
    10381 tcttaaggtt gatgacgagg gaaagctgat accgatatca aagaataagg gaagaaagaa
    10441 atccattaag acgctttttg aaatgcttca ggaaaatata gaggctcctg aggagcaggt
    10501 aattgccatc agccacgggg attgcatcga ggaagcagaa tatcttaagg aaatgatact
    10561 gaaggaatat aatgtaaagg atgtaataat aaatcatgta gggccggtta taggagcaca
    10621 taccgggccg ggtgtagtgg cacttttctt ctttggaaac aaaagataga gggtatacaa
    10681 taacaggggg acggaaatgt cccattccat agggacataa tgtgaaccgt gggtaatttg
    10741 ccgaggcagg cagcagatta agctggctgc ctttactgtt gccccagtac tttctcggct
    10801 tctttggcac ttttcggaga tagttgctaa atttggcata aatatgataa ttctgacttt
    10861 ttttgtgtta aatagatgta ttcgagcaag ggacgtgata taatcaacat aggtaaatcc
    10921 ggaagagatt tatttcgagg taaattacca tggcggtaag ctgcaataaa ctttggacgc
    10981 tgcttattga cagaaaaatg aagcaaatcc tcaatccatg ttgattgctt agctcggaag
    11041 gaagtaagtt catgaaaaag cgaagaacac ttaagcgtga gcgcattaac cgtgaactca
    11101 cgaatatatt taaatatccg ctgacgattg tggaagcgcc catgggctac ggcaagacca
    11161 ctgcagtgag tgaattcctt gctttaaaag gcgtgcctgt catatggact tcctttttat
    11221 ccgaagaaga cacggcgcat ggtttttggg aaaggcttgc ggccgaaatc ggtaaatttg
    11281 ataaggcggc ggagagcagg cttaaaagtc tcggcgttcc tgcagacaca ccgcagacaa
    11341 caatggctgt ttccatcatc aatgagatgg gttacaagcc cgataccacg ctcgtcatcg
    11401 acgatttcca ccttgcaaga agtatgcggg tgactgcact tttcagacgc tttgtaatgg
    11461 aaatgccgga cgattttcac attgtcatct tgacccgtga tacgacaaat cttgacatca
    11521 ccgagctttc cgcaaaaggg ctgtgctata ttgtgtctca aaatatgctg cggtttacag
    11581 acagtgagat tcgggactat tgcgccctga tgggtttcac ggccagcgaa ggcgagataa
    11641 gaaaaatcgg agattacacg ggcggctgga tttcattgat ttatcttatc atgctcggta
    11701 tggaacgggg cattcccgtg ggccgaaacg gcacaatcaa tgagttggtg gagaaggtgc
    11761 tctataacgc atacgatgag cgcatccaaa ggtttttgct gtgcctttcc gtgatggaca
    11821 gcttcacggc ggagcaggct gtgtttgtaa cgcaagaaga aaaatcggag gaaaccctta
    11881 aaaagttgcg ccgcgaaaac gcatttattg ccttcgacga ggcggcaggg gtctatataa
    11941 tacacaatgt gctgctggat ttccttcggg gtaagcagaa ggatgaagaa gaacgtgccg
    12001 cgctctatcg tcggcttggc gaatggtacc ttgcacaaag gaaatataga actgcatacg
    12061 gttatctcta ccgcgcgggt gagtcggagc gaatcctcgc gctgctggac aacggggata
    12121 ccatcaccag cgattccgcc acttttgacg gcgctttaga cttgttctcc gcaatgcccc
    12181 gggagatgct tcataaatat ccgcttgctt atttgcagta tatcgccatc attttaacaa
    12241 acggcgatcc cgacgatttt caggacggtg tgatacgctt aaacgagttg cgggaggctt
    12301 gcgagcgggc ggaagacccc gtcccgcacg gcagagaccg cgttcttgcg gaactttatc
    12361 tcactcagat ttttgccgtg ttcaatgatg ctaaggaaat ggccgcttgt atagagaagg
    12421 cacagcgcct gcttgacggt gatgtttcct tcttgcaaaa gcgggaagca gagttcacct
    12481 tgggctctcc ccatcttctc tatacctgtt acagggagcc gggcagcctc aaagagctta
    12541 cagagcttat ggcatcggat tttccgattt ttgccgagct tgccgatggc tgcagcaccg
    12601 gctgcgacta tgtggttcga gccgaatacg cgctggaaac cggcaactgg caggcggcgg
    12661 agttggatgc ctttaaaggc atttataaag cgcagacaaa ggaacagatc ggtcttgtga
    12721 tctgcgctta ttttgttttg atcaggctgt atctctataa ggggaagacc aatgaagcat
    12781 tggagcttct gcggcagctg aggcaggatg tgaagcggac gaacaacctc tattacaaca
    12841 ccgggcttga gatcgtggaa gggtacgttt acggctgcct cacccggcta aacagcatcc
    12901 ccttgtggct gcaaaccggg gatatgtccc ccgcgcaatt tttttacgag ggtctgggct
    12961 ttaaatatat cgtctacggc aaagcgcttc tgctttcaaa gaattacatc aaactcgaaa
    13021 tgctcactga agaatttgcg cgctgtttcg ccgtcttcca caaccagctc ggctttctgc
    13081 acaatcagat tttcagggca actgcgaaat atcctcttta cggcatggag gttggctgcg
    13141 cagccctccg cgaagcattc agcatggccc gggaagacca catcatcctg cccttcgccg
    13201 agtatgcgcc cgccgtcata gacatggtca ggcacatcgc ccatgctgat tcgcgggacg
    13261 catacataaa gaaggttctt gaagcctgcg tgcattatat ggagagcctg aagcactcta
    13321 tacagagcgc agtttccctg tcggcaagag agcttgaaat ccttaccctg gcagcggagg
    13381 gcctcaaaag ggacgagatt gccggaaagc ttcatgttac cgcaggcacc gtacagactc
    13441 atctgcataa tatctacctg aaactggagg tcagcggcag gatggcagcc ataaaaaagg
    13501 cgcaaaagtt gaagcttttg taatttttcg aaaaaatata ccgttcggta gatatagaaa
    13561 taaaaaatta tcccctatac ttctcagtat gggggatttt ttatgtccga aacatccgcc
    13621 aaatagaaga aggagacttc gtattatgag taaaatactc aaggtcacgc ccaatgaaga
    13681 ttataccctg ctggtggaat tcgagcacgg caacaagatc atcttcaata tgcgcgatat
    13741 cataaaaaca atacctttca gcagtctgga agatttaaac cgtttcaaga acgtcacagt
    13801 ggaggaaaaa gccataagct ggcccgagcc ggtaccgggc gagaaaagta ttatgccctt
    13861 gcgtctgacg gtggataaca ttctgttcgc catacgggat taaaacattc agaaatgaat
    13921 tgagaattga tataagaaag ggggaccatt taacatggta aagagaataa taagtttttt
    13981 gcttgtgatg tctatgttgc tgctcgcact gccaacggct gtgcttgcgg acagtttaaa
    14041 acaagaaccc gaaaatgagt acatagatgt taacaaagcc gactggtttt atgaatctgt
    14101 gatgtattgt ttggaaaatg gcatttttgg cggtaccggt gaaggtacat tttcgccggg
    14161 tacttccttg acgcgcgcga tgtatgtgac cgtgctggga agggttgcgg gtgttgatac
    14221 aagccaatac aacacatcgg cgtttaaaga tgtggcggtt ggcgcttggt atgcacctta
    14281 tgtggcatgg gcagcagaaa acggtatcac ccacggcaca gacaatgata ctttctcgcc
    14341 ggatgaagct attacaagag aacagatggc aacaatgacg gtaagatatt ttaaaagctg
    14401 taatatatct tacgaagctg aaaggcagat agcatccgga cctaaagatg cacccgccat
    14461 ttcttcatgg gctcttgatt ctgtattaag cctttggcat gcggggctgc ttgcaggaga
    14521 tgagaatggc aattttaacc ctgcatccaa cgctacccgc gctgaagctg cagctttctg
    14581 catgcgcata agcaagacgg tgaagcagca agaggagaaa aaacaaccga cgccggcacc
    14641 ggcgccgaca ccggcgtcat cacagggctc aggaaacgat agggacgaaa gcagatctga
    14701 cactaaatat accattactt tcgtaacaaa tgggggagat gcaattgata agctgagact
    14761 aaaacccggg accgcacttg acaatctccc ggttccatat aaaatggacg ctgttttcct
    14821 gggttggtat aaggacagca gcctttcaac gccggtgagc agcaccgaca ttattaccgg
    14881 aaacatgacg ttatatgcca aatatgcggc ttcaaccggt ttcaccgaag agctgagcgt
    14941 cccgacgata agcaagcttg accaggataa gacctttaca ataatagttg tggattcaac
    15001 agacggaatg accgcagcgg aagtcaaagc gggcatgacc tttgattctc cctcaaatcc
    15061 cggttttgcg ggcattcaag tggatgaatc ctctgacgga gaattcatcg tatctgcaat
    15121 tggaggcgct tttgacgagg gcggcacctt taagctgact ctcgataacg acaacctttc
    15181 cttcaaggga gaggacgaat ccacgaggat ttgcactttc accattgcca agtcgcccgt
    15241 gatgaatctc agcctcaaca agaatatgaa atacctatct gccggacaga tatccgattt
    15301 gacgcaaaat ggcttttccg tcaaatcact atccattccg ttggtacggg tagccggcga
    15361 cggcgaaggc ctgagcggta ttgacgccga ggggggtacc tttgtatatg acggcggcgg
    15421 catcaatgtg ggcgatacgg ttgccattta tgagggaatc aggccggacc aacgcgatct
    15481 tgatacgagc ggtgacgatg cgggagacat tgcctatgtt actatcactg ccatcagcgg
    15541 cacaacctat tcctatgtaa aagcggatga aaaggatatt ttatttacgc cggatgtcct
    15601 gccggtcagt accacagcag acacggacgg ctcttccgat aacaactccg tcaccgtccc
    15661 tcaaagtgtt atggcttata ccgatgccat atatgcggat attggactta acgccgctac
    15721 cactgttgac gtcggagact ttatcgcctt ccataccggc acctttggat cggcggatgc
    15781 ggaaaatgcc ggttatgcgg aaatcacgtc tgtgactgtt tccggagata actatattat
    15841 agcctacata tccacgacac cggagcagat gtgggaggca acggatctgt ataccacaaa
    15901 ggaagttgat ggcgatgaac tgcttgaaaa cgtggatata gccgcgttag aagagagcgt
    15961 tgagcggcag gcgatagaga gcgggtttgc ggacgaagcc gcccagtatc tggcggagat
    16021 ggcgatgaag acagatagct ttaggcagtt gaatcttacg gctatggctg acacgtcctc
    16081 ctatggtgct atgtccatgc ttgaagcttt cggcaccggg cctgaagtga aaaatctcaa
    16141 ggttaacgct agggtcttca attatctcca gcatctaggc ggaagcggcg cccgtgtgac
    16201 gctgaccatc agctgcgata tcgtgtttca ggcggcagga gaggataata atattgtcat
    16261 acgtctgtcc ggcgcctttg agcaagaggt aaaggtcgat ctgaatgtca gctgcgaaac
    16321 gaaatggaca ttattagatg gatggttttg gtgggctgaa gacactcttg tgaccgcgag
    16381 tatcgatact ggaacttata ccagtatcaa tatcaacgcc agcataggca cttttgaaaa
    16441 aggagcgcaa tgtgacttaa gtaagttcca ggatattggt aagcagataa aagacatcat
    16501 gaacggcaaa aagccggagg acagtgctat agctgatacc ctccgggaaa aatacaaagc
    16561 catgcttgaa gatgacagcg actgggtcga catatacaaa aaggagatat ttacctttga
    16621 acaggcgatt gaccccttcc acatcgtagt cttcgggatg gatgtagact ttgtggtagg
    16681 cgcaaaaatg aacgtgtcca ttggctgcaa tttctcctat gaaagcgaaa agagatatat
    16741 attctcgcta tatataaatg cccagactgc caccagcgat acaatagatc ttaggccgga
    16801 gcagtataac ttcacgtttt atattatggg aacgctgggc ttgcgcgcgg gtatcagcat
    16861 tgagattaaa gtaggtttgt tttctttaga cttggcgagt ataggtattg aaggcgaggt
    16921 gggcgcttat gtgcaattat ggggatattt ttactacgag gcaagttatc ggcaagcaac
    16981 aggcttgacg tccaaatatg gcggcgcgat gtacctggaa ttcggcattt atcttgaaat
    17041 aagctttgtt gcgcaggcga ttgacaatat gtttgaatac gaggcgacgc tttatgaaaa
    17101 ccagtggccg ctgctaacct tcgggtcgca ggaggatgtc tatgactttg agtatagtgc
    17161 cgataaggca ccaaccgctt tcatggccaa gcagacaaaa agctttgtcc tgccggacag
    17221 cgtcttcagc atgaattata tggatatgaa aacgggagat attgaaagca aaaattatga
    17281 cccatctaat tttgattata gtactacaaa cagcgatttt acgtacgatc cggcgacgaa
    17341 aaccgtcaca gttgcgccag gcgaaaagga catcaccgaa ggtgcgttgg ttctcatatg
    17401 gaagggcaat cctgttgcct tcacttccgt gcccattgcg cgcgcggtcc cgctgcattg
    17461 ggaagacctg aaaagcgcgt atactataag cttcaattca cgcggaggca gcgcggtgga
    17521 caccatcgca ttaccttata aaggagtaat tacacctccg gcgaatcctg caaaggctgg
    17581 ctatgtattc ggcggctggt ataaggacga agccttgacg caaagctata ccttccctgc
    17641 cacgatgccg gctgaggaca ctgtactcta tgccaggtgg gtaccggctc ctaacactaa
    17701 gtacagggtg gagcattata agcagaacct cgaaaacgac ctgtacacgc tggcagaaac
    17761 agaatatcgt acaggcacga ccgactcagt agttcacgtt tcaccaaaaa gctatgaagg
    17821 attttcttta gtgccatcac cggaaatcgt ccagattttg cctgacggca gcactgtgca
    17881 tgtgtttcat tataaccgca acagctatag cgcaacattc caaccgaata acggtaagat
    17941 tggcgcggac gtcgaaaaca cagtcaagtt cggcggaaaa atcaacatac ctgtctttac
    18001 aagggcaagg cactcttttg ccggctggtc tccggctgtc cccgagacaa tgcccgcgca
    18061 gaatatgact ttcgaagccc aatggacgac ggtggggcag gtagcctata aggtcgaaca
    18121 ttacaaagaa ggcttggacg gcagctatgc cttagccgaa acggaaaacc tcaccgacgt
    18181 cgcaaatcag atggtaacgg caacagcaaa aagctatggc ggatttagct ataaccgtaa
    18241 cgccccgggt acggttccgt caggtatagt cacatctgac ggcaatctcg tactcaagct
    18301 gtactataca cggaacagct atacgctgac ctttgacgct aacggcggta cgggaggcat
    18361 tacccgccag gcgaagttcg gcgaacctat cagcgcgccc gcagtcgcga gggaaggcta
    18421 cagcctgacc ggttggtctc ccgctgtccc ggccactatg ccggcggaga atgcaaccta
    18481 tacggcacag tggcaagctg ttgggctgat tccgtatagg gtggagcatt atcttgagaa
    18541 tctcgatgat ggctacattc ttacggatat cgaaaatcta agcggggctg aaggagatac
    18601 ggcgacggca agcacgaaaa gctataacgg gtttgaatat cttgccgatc ctccgaccgt
    18661 tgcttccgga acgatagcag ctgacggcag ccttgtcctc aaattgtact atatgcgaaa
    18721 agacgttacg ctggagtttt tggctgatgg cggcagtgcg ggagacaatg agcttgatta
    18781 ccaatacggc gactttttca atgaattccc gccgggaaca atagagggct atgcctttaa
    18841 cggttggtcc cccgccttta tgggaacagc tccggcggag gacacaacct ataccgcaca
    18901 gtggacgtca aatatttcgg aagcgcttga cgtaacgaca gcggtatatg ccaatcaaga
    18961 ctatacagcc ggcggtgtgt actgggatta cgaagcccaa acccttacat tgaccgacgt
    19021 agtaatcaca tcgccggatt ctgctgagag cggggttgac aattttgcct taaaggtacc
    19081 tgacggcacg actatcatat tggtcggctc caactatctg caatcgggta agggaccagg
    19141 aataaacagt tacggcattc tgtgtgacgg cgacttgata attcaggggc cgggcacgct
    19201 caccgctttg ggtacaccaa acttcatgag cttagccgac caaatcgttt gctatggtat
    19261 ttacgcagcc ggaaccatca ctatagagga cgacgccgcc gtaatagcta aaagcacggg
    19321 agctaccggg aacagctgcg gcatttttgc ggaagacgta gttatcacag acagcacggt
    19381 cttagcttca agtaatgaat cggcaaatgg catccgctgc ggtatttacg caagcgggga
    19441 tatagacatt accaacagta cagtaaatac gcccagcggt atttacgcat ccggcgacgc
    19501 ggcgattagc gaaagcacgg tcaacgcgct caactccgga attgaatacc ccgacgacgt
    19561 attcaactat tacggaatct atggggacaa cgtggcaatc accggcggaa cggtatatgt
    19621 cacttcgcag tctgctgatg gctacggaat ttacgccgcc gccggggtcg aaatcaccgg
    19681 cagcgcagcg gtaaccgcaa ggtgcagtga tttcggagga cccggcacag gcagcggtat
    19741 ttatgcgggg acaggttcaa ttagcatatc cggcagcacc gtcatagcgg caggcaagga
    19801 tgcgctggcg atgaacaagg ctcctgtact caataatgct gaggctttct gtgccagtac
    19861 aagcgcagac ggctcgggcg gagtcatcta tgattcaggc gacatagcaa catataaata
    19921 tatacaaata cgggctgact gatgctgatt gccggaaaac agataaagca aggcacttcc
    19981 ttcgaagtgc cttgct
//
LOCUS       DAOQUR010000041        19601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3233_length_19601_cov_10.303643, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000041 DAOQUR010000000
VERSION     DAOQUR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19601
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_19601_cov_10.303643"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="sigE"
                     /locus_tag="PKI50_08885"
     CDS             complement(<1..722)
                     /gene="sigE"
                     /locus_tag="PKI50_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HNT03854.1"
                     /translation="MKKTAGRLSLIKRMLIIRILQFFKVYKETWVHYIGGSEALPPPL
                     ENEEENFLLSRLKGDEEVVKAVLIERNLRLVVYIARKFENTGVGVEDLVSIGTIGLIK
                     AVNTFDPEKKIKLATYASRCIENEILMYLRRNNRTKTEISFDEPLNIDWDGNELLLSD
                     ILGTDNDIIYRYLEDEIDKQLLEIALRKLSEREKRIMELRFGLNDGVEKTQKEVADML
                     GISQSYISRLEKRIIKRLKKEI"
     gene            complement(771..1679)
                     /gene="spoIIGA"
                     /locus_tag="PKI50_08890"
     CDS             complement(771..1679)
                     /gene="spoIIGA"
                     /locus_tag="PKI50_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HNT03855.1"
                     /translation="MKLETYVYADIILLENLIMNYLILWSTARLARYRHSKVKLLIAS
                     LLGAVYAVLSYFPGYGYLFTFYMKILFSLLIVIVAYTPAYFHLLLKLTGIFYIISFIF
                     GGAAFGLFYFINGLNLTSNGISFIRDFPVKILIAAVTAAYFIISYSWDYIHHRIKREK
                     IILKVQMRFDGKQLCMDALVDTGNSLKDPLTNTPVMITEYDTIKELLPDDVQKIFEQE
                     GENDLNAIAEIMTISKWASRFRIIPFRSLGRENGMLVGFKPDEAIIFDKDRRIQSDII
                     IAIYRRNLSGDGEYGALIHPEMLKDR"
     gene            complement(2326..3411)
                     /gene="ftsZ"
                     /locus_tag="PKI50_08895"
     CDS             complement(2326..3411)
                     /gene="ftsZ"
                     /locus_tag="PKI50_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNT03856.1"
                     /translation="MLEFDVDINSIAQIKVVGVGGAGNNAINRMINAGLKGVEFYAIN
                     TDKQALHMSLAPNKIQIGEKLTKGLGAGANPDIGKRAAEESYDDIKKVLEGADMIFVT
                     AGMGGGTGTGAAPLVAQIARELGILTVGVVTRPFGFEGKVRKMQAEKGLTELKEKVDT
                     LVTIPNDRLLQIVDKKASIMEAFRIADDVLRQGVQGISDLIAVPGLVNLDFADVKTIM
                     LDKGYAHMGIGKANGENRSSEAVKQAIHSPLLETCIEGAKSVLLNITGGADLGIFEVN
                     EAADLVFQSADPDANIIFGAVIDESMQDEIRITVIATGFEEGAQRFFERRIEVAGLPE
                     EKGDGQKISNYGNEDIDIPTFLRNRYK"
     gene            complement(3529..3870)
                     /locus_tag="PKI50_08900"
     CDS             complement(3529..3870)
                     /locus_tag="PKI50_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HNT03857.1"
                     /translation="MIIPIIGLLLGIVIGILSPITIPIQYSSYMSVAILAALDSVFGG
                     IRSSIEKTFNIEIFMSGFFGNAVLAAILTYIGDQLGVPIYYAAIFAFGVRLFQNFAII
                     RRYVLMEHKKK"
     gene            complement(3870..4598)
                     /locus_tag="PKI50_08905"
     CDS             complement(3870..4598)
                     /locus_tag="PKI50_08905"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HNT03858.1"
                     /translation="MNRKAYIYYLFILAVIIGFIVTVQLKSNITYQGIITIPKLLDLQ
                     NEISNVEMENKLLKESISEHAMRLVEYKTSVEETGSAYGTMQNELEKARNYAGFYAVQ
                     GPGIIMTLNDSQQELAEGEDIAWYLVHDIDILEIVNELRVAGAEAISINGERVTATTN
                     IRCGGPTINIDGKRHAAPFIIRAIGDPKALEASALAPDSYIELYMEYTGIQVEIQKVE
                     KLVINGQNNLGKMKYQRKAEGGEA"
     gene            complement(4600..5319)
                     /locus_tag="PKI50_08910"
     CDS             complement(4600..5319)
                     /locus_tag="PKI50_08910"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HNT03859.1"
                     /translation="MKNIKYQIAVAVVCMVLGIMVSIQFRTVKQGVGPVSEYRARELA
                     AQLKKVREENVKLQNVKNDYESKIKEFEDTASQGSLYAKILKEELDQARILAGIEDVE
                     GPGITVVVDDLKFSEKVNYPLISYSMLLELLNELNAAGAEAVSINEQRIISTSEIRQI
                     GGIHININTVSFAPPFVFKAIGDPKTLEAALRLREGIAEKLENSGVAVTITQEQLIKI
                     QKYNGVIERKYAKVVKEGESQ"
     gene            complement(5348..6064)
                     /locus_tag="PKI50_08915"
     CDS             complement(5348..6064)
                     /locus_tag="PKI50_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNT03860.1"
                     /translation="MRGRFKYVVLLFIICIFTAFFLSSSFFQIKYLVVNGNNNVTREE
                     IIKLSSIYYGENIFRINKRNSMKSIFQNPYVKMIKINRVLPDKVVIDIIERGIMAYVP
                     YVGSYLNIDEEGMILEINPAIKRTDLPLVKGLQFETFKVGEFLNVKNEEQFLTTTQLI
                     KVIKNAGMLNLVSEIDISDLSNIRLIIKEGIKANLGSADNMDYKINFARSVIEDVMKQ
                     NLKGTIEMSHNGNPVFKQEQ"
     gene            complement(6163..7416)
                     /gene="murA"
                     /locus_tag="PKI50_08920"
     CDS             complement(6163..7416)
                     /gene="murA"
                     /locus_tag="PKI50_08920"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT03861.1"
                     /translation="MDKYVIIGGRRLEGDVRVDGSKNSILPVLAATIISGKESVIHNV
                     PELKDVDLLIGLLRTIGCRCFFENNTLVVKSNSQLNTIIPEKPVREMRSSIILMGAML
                     ARHGHVKISYPGGCEIGPRPIDIHLSGLRRMGTKITEAHGYINCECDRLKGVEINLDY
                     PSVGATENIMLAATTAEGVTIIQNAAKEPEIVDLQNFLNGMGARISGAGTSTVKIEGV
                     KEFHDVEHTVIPDRIVAGTLMAAAAITGGNIVLNDVIIDHLKLVSSKLSESGCSITEY
                     QNSIQITCSKKPKAVEVIKTLPYPGFATDMQAQMMAVMTIAKGTSIFIETVFESRYKH
                     VEELMKMGANIKVDGRTAVVRGVKKLTGTEVKAGDLRGGAALVLAGLAAEGTTVVDNV
                     KLHIDRGYDKLENKLAKLGADIHRV"
     gene            complement(7534..8652)
                     /gene="murG"
                     /locus_tag="PKI50_08925"
     CDS             complement(7534..8652)
                     /gene="murG"
                     /locus_tag="PKI50_08925"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNT03862.1"
                     /translation="MRAILSGGGTGGHIYPAVSIAKEIKNLYTDSEILFIGTDRGLES
                     SIVPKEGFKLVKIKVRGFLRKFSFGNVTAVAESFTGLFKVSKIIKDFRPDIVIGTGGY
                     VSGSVLLAAALMGIPTLIHEQNAFPGITNKILARLVDIIAVNFPEAKEYFPRKDKVIV
                     TGNPIRSTILSVSKEEGLKEFGFSRELPVVLVVGGSRGARKLNESVMLLAKKCVESKS
                     FQMLHMTGETQYDDILKKYDNENIPLDIGYLRVMPYVYNMPYALTASDIIISRCGAST
                     LSEITALGKPSILIPYPYAAGNHQEYNARALEKNGAAIVIQDRDLDADILYNEIAGML
                     NKPEKVSQMAAKSKKLGRTDAAYSIAKAAERLVQNKKA"
     gene            complement(8658..9770)
                     /gene="spoVE"
                     /locus_tag="PKI50_08930"
     CDS             complement(8658..9770)
                     /gene="spoVE"
                     /locus_tag="PKI50_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HNT03863.1"
                     /translation="MPARSKNNIDFTLLITVLILLAIGVAMVFSASSISSFMKYNDRY
                     HYLKSQGFFAILGILAMMFMSRFDYRILGRFAGPLVLLSIALLIAVFIPGIGYTANEA
                     TRWIKIGSQTMQPSEFAKFALIIFMAKSISNKKEKIRSFKHGVLPYVILAAIYFVLII
                     LEPNLSMAGSILMITFAMLFAAGTKIIYLIGWLIPVLPTVGYIIMKKPYMLARVTSYM
                     NPWADPLNKGYQAIQSLYALGSGGIFGLGLGNSRQKFFYIPEPQNDFIFAIIGEELGF
                     IGTATIVILFLMLIWRGLRIALACPDTFGCLLATGITCMIAIQATLNIAVATVSIPTT
                     GISLPFISSGGSSLLFVMVNMGILLNISKTVKTGRS"
     gene            complement(9834..11216)
                     /gene="murD"
                     /locus_tag="PKI50_08935"
     CDS             complement(9834..11216)
                     /gene="murD"
                     /locus_tag="PKI50_08935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNT03864.1"
                     /translation="MQIKGKKVLVIGAAVTGVPVVKQLSRLGANIVLNDFKKVEELKE
                     VISEIQELPIKLVAGGHPARLAEDCDFVVISPGVPLDIPPVQRAKAMGKEVISEIELS
                     FRLTNTPIAAITGTNGKTTTTSLLGEIMRASGRKTYVAGNIGSAMINEVDKAKRPDIF
                     VLEVSSFQLESTIAFKPNISAILNITPDHLDRHKNMENYIDAKCRVFANQDEKDYTIL
                     NLDDPETSKLADRPKSKVLFFSRKKEVPQGVYMEKDYIVIKADNKETIIHKDKIYIPG
                     NHNLENALAAALMAYCAGVAPSVIADTLESFRGVEHRIEYVEELDGVIYYNDSKGTNT
                     DASIKAVEAMKRPTIIIAGGYDKGSEYDDFIEAFGDVVRHMVLIGKTADKLEETAHRH
                     GFMNTHKARDLREAVMKCHSLAEPGDCVLLSPACASWDMFSNYEERGRLFKEYVRELR
                     IRDQRYQAQN"
     gene            complement(11312..12289)
                     /gene="mraY"
                     /locus_tag="PKI50_08940"
     CDS             complement(11312..12289)
                     /gene="mraY"
                     /locus_tag="PKI50_08940"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNT03865.1"
                     /translation="MYNLVSGEYDIYIISIISAFVLALVAGPVFIPVLTRMKFGQIVR
                     DDGPQSHLKKTGTPAMGGIIIAVPAVIVSLAFSKDKDMLLVLITTMLFGIIGFIDDFL
                     KIKNRRSLGLKAYQKIILQLLVSVFLAVVASGISQVGTEVLVPFTGKFIDMGILYIPF
                     TVFVLISTVNCVNLTDGLDGLAGGTTVVVLGFFSVIALTSKNTGLLVFSGAMIGALLG
                     FLRFNSHPAQVFMGDTGSLALGGAIASLAVITRLPLFILIIGAIYVLEGLSVIIQVAY
                     FKMTGGKRFFKMAPLHHHFELSGWAESKIVAVFIISSIILSLIGILGLS"
     gene            complement(12296..13669)
                     /gene="murF"
                     /locus_tag="PKI50_08945"
     CDS             complement(12296..13669)
                     /gene="murF"
                     /locus_tag="PKI50_08945"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNT03866.1"
                     /translation="MMEALSMGEIARAAHGVLMNFEEESEISGVSIDSRKINPGDMFI
                     ALKGENFDGHDFIDMAIEKGAVLAVTQRLPERCKIPYILVEDTLKALQDIAAYYRSKF
                     NIPIVAITGSSGKTTTKDMIASVLTQRFNVLKTEGNLNNAIGLPLTLLKLQYSHQIAV
                     LEMGMSSLGEISLLSDIARPGIGVISNVGLTHIEKLGSRENILRAKLEIFSYFNSDST
                     AVINGDNDMLRDFCSDKFRVIKYGLKKGNDLFAYGIEEKGEAGIEFSVDLDGQSNFRV
                     LLPGIHNVYNALSAIAVARLFGMKAEEIQKGLDSFKPSEMRMEIIVLGSGVKLINDVY
                     NANPESMKVAIDVLRTMKSKGRGICILGDMLELGNLSSEEHCKTGAYAASSGADAIIA
                     VGEFSEDIKRGAAASGMNCNVYAFSDIKAAAASLDYIIRPGDTVLVKGSRGMKMEYIV
                     DYLRERG"
     gene            complement(13666..15123)
                     /locus_tag="PKI50_08950"
     CDS             complement(13666..15123)
                     /locus_tag="PKI50_08950"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNT03867.1"
                     /translation="MKLEELLTGVDTVSFKGDLNIEINGIIYDSRKAKKGDLFICVSG
                     YKYDGHSYINDALQKGAAAFIVEKDVEVPGAAVVKVENSRAVMPMLASNFYGNPSQKL
                     KLIGITGTNGKTTTTYLIKSIMEANKQKIGLLGTISTQIGDKIYISSRTTPEALDLQC
                     LFREMADSDVDYAVMEVSSHSLELGRVEGSNFRIGIFTNLTQDHLDFHKTIENYGNAK
                     KKLFYMVSGTSIINIDDEHGKIIAAELKKNGKKLITYGIDNAADIMAKNIDITSKGVG
                     FTVITPEYEAELNISIPGKFSVYNALAAASAAYAEGLDMEVIREGLSRVENVPGRSEI
                     IKTNTPYTVIIDYAHTPDGLQNILASVKEYARGRIITLFGCGGDRDKEKRPIMGNIAG
                     RMSDYCIITSDNPRTEEPMDIIMQIEAGIKSTGCDYICIENRRDAIKFALATAKPDDI
                     VLLAGKGHETYQILRDRTIPFDERDIVEELLREEV"
     gene            complement(15320..17293)
                     /locus_tag="PKI50_08955"
     CDS             complement(15320..17293)
                     /locus_tag="PKI50_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HNT03868.1"
                     /translation="MSSIKVTIKRRIVIIFFAFFVIYAALLGRLAFIQIVNSAEYQQK
                     AVEQWTRDIPIRSKRGIIYDRNFKKLAVSATAYEIYVRPAMVKDKDAVTNALSEILGM
                     DKASLAQKVTANKDTVLIKKKVDAEKVKLLREKELQGIVFNDDSKRFYPQSNFASYVL
                     GFTNPDNQGQDGVELTYEKYLNGFPGRNIVMTDAHGEMLPGSDTKYYEPQDGLNLVLT
                     IDEVIQHFAEKVVENALIENKAKRVTAIVMDPKTGDILAMTSKPDFDNNDPRKVPEDL
                     ADKWSTMSEKEQLDVLYRIWRNPAISDSYEPGSTFKVITSSAGLEENVVKPEDHFNCI
                     GHITVAGKKMKCWRSYKPHGNQSFAEAVKNSCNPVFVEVGQRMGKEKLYKYIKAFGFG
                     SVTNIKLPGEARGIVRELGGVGPVELANNSFGQGISVTPIQLITAFSAVANDGMLMEP
                     RIAKKVIDGEETTVHEFQPRAVRQVISRETSATMRQILEFAIPPTASSYIPGYKVAGK
                     TGTAQKAEGGIYVSGKFVSSFCGFAPANDPKVSVLVVIDEPGAGQYYGGVIAAPVARS
                     IIYDTLRYMDVKPQYTAEEKELMQRPLVKVPEVRNMALKNAIKELEKSKLKYSVEAEF
                     EHNMESTIVDQTPKPGADINEGSIVILNAKPVQ"
     gene            complement(17326..17823)
                     /locus_tag="PKI50_08960"
     CDS             complement(17326..17823)
                     /locus_tag="PKI50_08960"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNT03869.1"
                     /translation="MLRADNTAYDYSRYEQLEMEQVREIAASPRKKVHKKEKHKTHLM
                     SILFVFGLSIFIISRYAYMAEINYNIKNLEKEYSALVKENTDLNVQLMKSISLETLEK
                     KAIEELGMQYPDIQNQIAYVNVEIPAQPAGNEARDFYNIDDLQENKYIAYTKAVINNI
                     LKVLD"
     gene            complement(17836..18786)
                     /gene="rsmH"
                     /locus_tag="PKI50_08965"
     CDS             complement(17836..18786)
                     /gene="rsmH"
                     /locus_tag="PKI50_08965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNT03870.1"
                     /translation="MDFEHIPVLLNETLEYLDPKPGGIYIDGTLGGAGHSSEIAKRIV
                     PGGVLIGIDQDSNAIDAATRRLEAYKDNVIIVRDNFRNIKTIALQKGFKEVDGILLDI
                     GVSSHQLDEKERGFSYMQDGPLDMRMDTENGLNASDIVNNSSEQELIRILRDYGEEKW
                     AVRIAKFITEERKNSRIDTTFKLADIIQRAIPAAARRDGGHPAKRTFQALRIAVNDEL
                     QVLEEAVTNAARLLKPGGRLVVITFHSLEDRIVKKIFNNMEKPCTCPPQLPVCACGKE
                     PLLRVITKKPVTAGVEELKTNTRSKSAKLRAAERVSSKRG"
     gene            complement(18816..19247)
                     /gene="mraZ"
                     /locus_tag="PKI50_08970"
     CDS             complement(18816..19247)
                     /gene="mraZ"
                     /locus_tag="PKI50_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNT03871.1"
                     /translation="MFIGEYNHTLDPKNRVIMPSKFREKLGDSFVMTKGLDNCLFIYS
                     SRDWSIVEDKLKSLPMTNKDARAFVRFFFAGACECDLDKQGRMVIPNYLKEHANIDKE
                     LVIIGVSTRIEIWSKEEWNKFNNDANISYEDVAEKMSQLGI"
BASE COUNT         5023 a         4953 c         3444 g         6181 t
ORIGIN      
        1 ctgatttctt tctttagccg ctttataatc ctcttctcca gccttgatat gtatgactgc
       61 gatatcccaa gcatatccgc aacctccttc tgggttttct ccactccgtc attaaggccg
      121 aaccttaatt ccattatcct cttctctctt tcggacagct ttctcagggc aatttcgaga
      181 agctgcttgt caatctcatc ttcaagatac ctatagatta tatcattatc cgtgccgaga
      241 atgtcagaaa gcagcagctc atttccatcc cagtcaatat tcagaggttc gtcaaaggat
      301 atttcggttt ttgtcctgtt gtttctgcga agatacataa gtatctcatt ttctatacaa
      361 cgggaggcat aagttgcaag ctttattttc ttttcaggat caaaagtgtt tacagcctta
      421 atcagcccta ttgttccgat agataccaaa tcctcaactc cgactccggt attttcaaac
      481 tttcttgcaa tatataccac aagcctgaga ttcctttcta ttagaacggc cttgactact
      541 tcttcatcac ccttgaggcg cgataaaaga aagttttctt cctcgttttc cagggggggc
      601 ggcaaagctt cgcttccgcc tatataatga acccaagttt ctttatatac tttaaaaaac
      661 tgtaagattc tgattatcag catgcgtttt atcaagctca atcttccggc tgtctttttc
      721 atttcttaac ctccattttg tattgttgtc tgcaaatcct tgggcgcatc ttatctatcc
      781 ttcaacatct cgggatgtat cagggcgccg tattccccat ctccggagag atttctcctg
      841 tatatggcaa ttattatatc ggattgtatt cttctgtcct tgtcgaatat tattgcctca
      901 tcaggcttga agcctacgag cataccgttc tctctgccca atgacctgaa gggtattatt
      961 ctgaatcttg aagcccactt ggaaatggtc attatttccg ctattgcatt taaatcattt
     1021 tccccctctt gttcaaaaat cttctgtaca tcatcaggca gcaattcttt tattgtatcg
     1081 tattccgtta tcataacagg agtattggta agaggatcct tcagtgagtt tcccgtatct
     1141 acaagagcat ccatgcatag ctgcttcccg tcaaagcgca tctgcacctt gagaattatt
     1201 ttttctcttt ttatcctgtg atgtatgtaa tcccagctgt aactgataat aaaatatgca
     1261 gcggtaacag ccgcaataag tatttttacg ggaaaatccc tgatgaaaga aatcccgttg
     1321 ctggtcaggt tcaggccgtt tataaagtag aataaaccaa aggcggcccc gccgaaaata
     1381 aaggaaataa tatagaaaat acccgtcagc ttcagcaaaa ggtgaaaata tgccggagtg
     1441 tatgcgacta tcacaataag cagtgaaaaa agtattttca tgtaaaatgt aaaaagatac
     1501 ccgtatccgg ggaaatagga aagtactgca taaaccgctc ccagcaaaga agcaatcaga
     1561 agcttcacct ttgaatgcct gtatctcgca agccgtgccg tactccataa aatcaaataa
     1621 ttcattatta agttttccag caatatgata tccgcataaa cataagtttc caacttcatt
     1681 cccttatctt tcatatgcaa tatatttcag gacaggcttc tgacaacata taatccggat
     1741 atcctaaaac atattccttg attcatagaa tacgccaaga ttaccaggta tgataaatca
     1801 actgttatcc ggcgtattct atatatatcg cttatgataa ttatataaaa aacttcatga
     1861 aatacctgtc atttttagta gtcgaaataa attatattat catatttatc gacagcagcc
     1921 ttcaatgggc gttaggccaa ttacaactaa tatatatatt caaaaggcaa caaaaaaaga
     1981 accaaaaatt ggttcttttc agtttgcaga caaagcttaa aataggaaca gcaatgtata
     2041 gtcagaacat acttatacaa aaattgttaa tggcccttat gaagctcgac ttagaaacaa
     2101 ccgtatgcga ggcacggagg ccgagcaagc tgtcacggtc acggacgacc tttgacagcg
     2161 ctaggttttt cttagttgag cggaattaga gccatcaatt tttgtatacc tatgcgagac
     2221 tatacattgc tgttcttaaa tttgaggttt ttcatcagtc taaaaagaac caaaattaat
     2281 tggttcttta taattgctcc tattccccga aacctcgaag tacgcttact tatatctgtt
     2341 cctcaagaaa gtcggtatat ctatatcttc attaccatag ttggatattt tctgcccgtc
     2401 ccctttttcc tccgggaggc cggcaacttc gatccttctt tcaaagaacc tttgggcacc
     2461 ttcttcaaag cctgttgcaa taacggtaat cctgatttca tcctgcatgc tctcatcgat
     2521 taccgcaccg aaaataatat ttgcatccgg atccgccgac tggaacacaa gatccgcagc
     2581 ttcatttact tcaaatattc caaggtctgc tccgccggta atattgagaa gcacactttt
     2641 tgccccttca atgcaggttt ccaaaagcgg gctgtgtatt gcctgtttta ctgcctcgga
     2701 acttctgttt tccccgttgg cctttcctat tcccatatgg gcatagccct tgtcgagcat
     2761 aatggtcttt acatctgcaa agtccagatt taccaggccg gggacggcaa taaggtcgga
     2821 aataccctga acaccctgcc tcaaaacatc atccgctatc ctgaaggcct ccattataga
     2881 cgctttctta tctacaatct gaagcagcct gtcatttgga attgtaacaa gagtatcaac
     2941 cttctccttc aattcagtca ggcctttttc ggcttgcatt ttccttactt tgccttcaaa
     3001 accgaaaggc cttgtgacta ctcctaccgt aagtattccc agttctctgg caatctgggc
     3061 aactaaaggc gcagctcctg tgccggtgcc gcctcccata cctgccgtaa caaatatcat
     3121 gtccgccccc tcgagaactt tctttatatc gtcataactt tcttcggctg ctctcttgcc
     3181 gatgtccgga tttgctccgg cacccagccc ctttgtaagt ttctcaccga tttgtatttt
     3241 atttggagcc agcgacatat gtaaagcctg tttatccgta tttatggcat agaactctac
     3301 tccctttaag cctgcattaa tcattctatt tattgcattg tttccggctc cgccaacccc
     3361 aacaacctta atctgcgcaa tagaattaat atcaacatca aattcaagca caatataccc
     3421 tccctatcta caactattat atatgcgaaa aattgtatac caagttgatt catcttaata
     3481 agttcaacat atctcttgat tttcctttat tttttttaac ttttatggtt attttttttt
     3541 gtgctccata agtacatatc ttcttattat tgcaaaattc tggaacagcc tgactccgaa
     3601 ggcaaatatc gctgcgtagt atataggcac tccaagctgg tctcctatat atgtaagtat
     3661 ggcagccaat actgcattgc cgaaaaatcc ggacataaat atctcaatat tgaaggtctt
     3721 ttctatactg gaccttattc ctccgaaaac cgaatccaaa gccgcaagaa ttgctaccga
     3781 catgtagctt gaatattgaa tcggtatggt aatcggcgaa agtattccaa tgacaattcc
     3841 cagcaatagt cctataattg gtataatcat tatgcttcac cgccttcagc ttttctttga
     3901 tacttcatct tacccaggtt attttggcca tttatgacaa gcttttctac tttttgaatc
     3961 tccacctgta taccggtata ttccatgtaa agctctatat agctatccgg agccagggca
     4021 gaggcttcaa gggcctttgg atcacctatt gcccttatga taaaaggagc tgcatgcctt
     4081 ttaccgtcaa tatttatcgt tgggcctccg catcttatgt tggtagtggc ggttactctt
     4141 tctccgttta ttgatattgc ttctgctcct gccactctaa gctcattgac tatttcaagg
     4201 atatctatat cgtgtaccag gtaccatgca atgtcttccc cctcagcaag ctcctgctgg
     4261 ctgtcattca gggtcattat gattcccggc ccctgaacgg cataaaagcc tgcataattc
     4321 ctggcctttt ccagctcatt ctgcatggtt ccataggcac tccccgtttc ttccacgctt
     4381 gtcttatact caaccaatct cattgcatgt tcgctgatgg actccttaag cagcttgttt
     4441 tccatttcca cattgctgat ctcattctgc aaatccagga gctttggtat tgtaatgata
     4501 ccctgatagg taatattgga ttttagctgc actgtaacaa taaagccaat aatcaccgcc
     4561 agtatgaaca aataatatat atatgccttt ctattcatat tattgggatt ctccctcttt
     4621 aacaactttt gcatatttcc tctcaataac tccgttgtat ttctgaattt taatcagctg
     4681 ctcctgcgta atcgtcacag ccactccact attctcaagc ttttctgcga tcccttccct
     4741 cagcctcaat gccgcttcca gagtctttgg gtctccgatg gctttaaaca caaagggagg
     4801 ggcaaaggag acagtgttga tattaatgtg tattccgcct atctgacgta tttccgaagt
     4861 ggatatgatt ctttgttcat ttatggatac tgcttcggca ccggcagcat taagttcatt
     4921 taaaagctcc aaaagcattg aatatgaaat caggggataa ttcacctttt cgctgaattt
     4981 caaatcgtct acgacaacgg taatccccgg gccttcaaca tcctctatgc ccgccagtat
     5041 ccttgcctga tccagttctt ccttcaatat ctttgcgtat aaactgcctt gagatgcagt
     5101 atcttcaaac tccttgatct ttgattcata atcgttctta acattctgca gcttgacatt
     5161 ttcttctctg acttttttaa gctgtgccgc cagttccctt gccctgtatt cagacacagg
     5221 gccgactccc tgctttacag tcctgaactg gattgaaacc atgataccca gcaccataca
     5281 tacaactgcc actgcaattt gatatttaat atttttcatt atccggtcct cctattatcc
     5341 ccactgttca ctgctcctgt ttaaaaacag gattgccatt atggctcatc tctatagttc
     5401 ctttaagatt ctgcttcatt acatcttcaa ttaccgacct ggcaaaatta atcttgtagt
     5461 ccatgttatc ggcagaaccc agattcgctt ttatgccttc cttaattatc agcctgatat
     5521 ttgataagtc cgagatatca atctctgaaa ccagattcag cattccggca ttctttatca
     5581 ccttgataag ctgagtagtt gttaaaaact gttcttcatt tttaacattc agaaactctc
     5641 cgaccttgaa ggtctcaaac tgcaatccct taaccagcgg taaatcggta cgcttaattg
     5701 cagggttgat ttccagtatc atgccttcct cgtcgatgtt gagataagac cctacataag
     5761 gtacataagc cataatacct ctttctatta tatcaataac aaccttatcg ggaagaactc
     5821 tattaatctt aatcattttg acgtaaggat tctgaaaaat actcttcatt gagtttctct
     5881 tatttattct gaatatattc tcgccgtaat atattgatga aagctttatt atttcttctc
     5941 ttgttacgtt attattccca tttacaacca gatactttat ctgaaaaaag ctggaagaca
     6001 gaaaaaacgc agtaaatatg cagattatga ataataaaac cacatatttg aatcttcccc
     6061 tcatgttttc actcctaaaa ctacaatatg tgcttgtccc aagaaaactt aaaaataaca
     6121 ctgcatgcag ttccgtatgc atacaggtta atattgaaaa tgctatactc tgtgtatgtc
     6181 agcacctaat tttgccagtt tattttctag tttatcatat cctctgtcta tatgcaattt
     6241 aacattgtcc actacagtag tcccttctgc tgcaagcccc gcaagcacca atgcggctcc
     6301 gcccctcaaa tcgccggctt taacctcggt gccggtaagc ttttttaccc cccttacaac
     6361 ggcagtcctt ccgtccacct ttatatttgc tcccattttc ataagctctt caacatgctt
     6421 gtatctgctt tcaaataccg tctcaatgaa tatgcttgtg ccctttgcta ttgtcattac
     6481 ggccatcatc tgagcctgca tatccgttgc aaatcccggg tatggaagag tctttatcac
     6541 ctcaaccgcc tttggttttt tgctgcacgt tatttgaatg ctgttctgat actcggtaat
     6601 actgcatccg ctttccgaaa gctttgacga taccaatttc aaatgatcaa ttattacgtc
     6661 atttaaaaca atatttccac ccgttatggc ggcagctgcc ataagtgttc ctgcaactat
     6721 cctgtcaggt atgacggtat gttccacgtc gtgaaattcc tttaccccct caatcttcac
     6781 agtactggtt cccgctcctg atattctggc tcccattccg ttgagaaaat tttgcaggtc
     6841 aacaatctcc ggctccttgg cagcattctg tattatagtt accccttcgg ctgttgtagc
     6901 cgcaagcatt atgttttcag tggcgcctac acttgggtaa tccaggttta tctccacacc
     6961 tttaagcctg tcgcactcac agttaatata tccgtgagct tccgtgattt tagtgcccat
     7021 tctccttagg cccgacaaat gaatatcaat ggggcgcggc cctatttcgc agcctccggg
     7081 gtaactgatt tttacatgcc cgtgacgtgc aagcattgct cccattagaa taattgagga
     7141 tctcatttcc cgtaccggtt tttccggtat aatagtattc aactggctat tggattttac
     7201 gactaatgta ttgttctcaa aaaaacacct gcagcctatt gtccgaagaa gtccgatcag
     7261 caaatcaacg tccttaagct ccggaacatt atggataacc gattctttcc cacttattat
     7321 ggtagcggca agtacgggca aaatagagtt cttggatccg tcgaccctta catctccttc
     7381 aagtctgcgc ccgccgatta tcacgtattt atccatcaac gatttacacc cccgggaagc
     7441 cattattctt caatcatatt aatatattat gcttcacttc ccaaggtgtt actgcaattg
     7501 caacgggcca ggcagtatca agccttgcaa atgctaggct ttcttatttt gaacaagcct
     7561 ttcggcagct tttgctattg aataagccgc atcagttctg cccagtttct tgctttttgc
     7621 tgccatttga cttacttttt ccggcttgtt caacatacct gcaatctcat tatacagtat
     7681 atcagcgtcc aagtccctgt cctgaataac aattgcagcc ccgttcttct ccagtgccct
     7741 tgcattatac tcctgatgat tgccggcagc ataaggataa ggtatcagta ttgagggttt
     7801 gcccaaagca gttatctccg acagggtgct ggctccgcac ctgcttatta ttatatcgct
     7861 tgcggtcagg gcatagggca tattgtaaac atagggcatt accctgagat atcctatatc
     7921 cagagggatg ttttcattat cgtatttttt aagtatgtca tcatattgag tctctccggt
     7981 catatgcagc atttgaaagc ttttcgactc aacgcacttt ttggcaagca gcatcacgct
     8041 ttcattcagt ttcctggctc ccctgcttcc cccgacaaca agaactaccg gcagttcccg
     8101 gctaaaacca aattccttaa ggccttcctc tttgcttaca ctcagtatgg tgctccttat
     8161 cgggttcccg gttacaatca ccttgtcctt cctgggaaag tactctttag cttccggaaa
     8221 attgactgca ataatgtcaa caagccttgc cagaatctta ttggtgattc ccgggaaggc
     8281 gttttgttca tgtatcaggg tcggtatacc cataagtgcg gcagcaagca gcaccgaacc
     8341 gcttacatat cccccggtgc caattactat atccggcctg aaatccttta ttatcttaga
     8401 taccttaaaa aggccggtga aggattccgc caccgctgtg acattcccaa agctgaattt
     8461 ccgcaggaag cctcttacct ttatttttac cagcttaaag ccttccttgg gaacaatgct
     8521 gctttccaat cccctatcgg ttcctatgaa cagtatttca gaatcagtat acagattttt
     8581 aatttctttg gcgatggaaa ctgcaggata tatatgtccc cccgtaccac ccccggaaag
     8641 tatggctctc attggattca actcctgcct gttttgactg tttttgagat attcaataaa
     8701 attcccatgt tcaccataac aaacagcagc gacgatcctc ccgaactgat aaagggaagc
     8761 gaaatccccg tagtaggtat ggatactgtc gccacagcta tgttaagagt agcctgtatt
     8821 gctatcatac atgtaattcc ggtggcaagc aggcaaccga aggtatcggg acaggccaaa
     8881 gctatcctca aacccctcca tataagcatg agaaacaata tcactattgt tgcggttcct
     8941 atgaatccca gttcctcccc gataattgca aatatgaagt cattttgcgg ctccggtatg
     9001 taaaaaaact tctgacggct gtttccaagc cccaatccga atattccccc cgagccgagg
     9061 gcataaaggg actgtattgc ctgataacct ttattcagcg gatcagccca gggattcata
     9121 taagaggtga cccgcgccag catataaggc tttttcataa ttatatagcc tactgtcggc
     9181 aggactggta tcagccagcc tatcaggtaa atgattttcg tccctgctgc aaaaagcatt
     9241 gcaaaggtta tcatcaaaat gcttcccgcc atactcaagt ttggttcaag tattatcagc
     9301 acaaaatata tggccgccaa aatcacgtat ggcaggactc catgcttgaa gctcctgatt
     9361 ttctccttct tattgcttat gctttttgcc atgaaaataa tcagtgcaaa ctttgcaaac
     9421 tcagatggct gcatcgtctg tgacccgatc tttatccacc tggtggcttc attggcggtg
     9481 tatccgattc cgggtatgaa tactgcgatt aagagggcta tgctcaatag caccagcggg
     9541 ccggcaaatc tccccagaat cctgtaatca aacctgctca taaacatcat agccaggatg
     9601 cctaatatgg caaaaaaacc ttgagacttc agatagtggt acctgtcgtt atacttcata
     9661 aaggaggaaa ttgaactggc ggaaaaaacc atagccacac ctatagccaa taatatcaat
     9721 accgttatca gtagtgtaaa gtcaatattg ttcttactcc ttgcaggcat caaaagccct
     9781 cctaagataa tataattgga ctgccatcgg ccgcccctgc tctaaagact tgcttaattt
     9841 tgcgcctggt atctctgatc ccgtatccga agttccctta catattcctt aaagagcctg
     9901 cctctttcct cataattgct gaacatatcc caactggcac aggccggcga aagcagcacg
     9961 cagtcgcccg gttcagcaag tgaatggcat ttcatcacag cctccctcag gtccctggct
    10021 ttatgcgtat tcataaaccc atgcctatgg gctgtttcct ccagcttatc ggcagttttt
    10081 cctatcagca ccatatgcct gaccacatcc ccaaaggcct cgatgaagtc atcatattcg
    10141 cttcctttgt catagccccc tgctattatt atagtaggcc gcttcattgc ctcaacggcc
    10201 ttgatggaag catctgtgtt ggtacctttt gaatcattat aataaattac tccgtcaagt
    10261 tcctcaacat attctatcct gtgttcaaca cccctgaagc tttccagggt atcggcaatt
    10321 actgaagggg ccacccccgc gcaatatgcc atgagagctg cagctaaagc gttttccaaa
    10381 ttgtggtttc cgggtatata gattttatcc ttgtgtatta ttgtttcttt attatccgcc
    10441 ttgattacta tataatcttt ttccatatat accccctggg gcacctcttt ctttctgctg
    10501 aaaaaaagca ccttgctttt tggcctgtct gccagcttgg aagtctccgg gtcatccaga
    10561 ttcagtatgg tataatcctt ttcatcctgg tttgcaaaaa ccctgcattt ggcatctata
    10621 tagttttcca tgtttttatg cctatccaaa tggtcgggtg taatattcaa tattgcagaa
    10681 atattcggct taaaggcaat cgtggattcc aactggaagc tgcttacctc cagcacaaaa
    10741 atatccggcc tctttgcttt atccacctca tttatcatag cagaaccgat attgccggca
    10801 acataggtct tcctcccgct tgcccgcatt atttccccaa gaagggacgt agtagtggtc
    10861 ttgccatttg ttccggttat tgcagcaatg ggtgtgtttg tcaggcggaa ggacagctca
    10921 atctcactta tgacttcttt gcccatagcc tttgccctct gcaccggcgg aatatccaaa
    10981 ggaacaccgg ggcttataac aacaaaatcg caatcctcag ccagccttgc cggatgtccc
    11041 ccggccacca gctttatggg cagttcctgg atttccgaga ttacctcttt aagctcctca
    11101 acctttttga aatcatttaa tacaatattc gcacccaacc ggctcaactg tttaacaaca
    11161 ggtactcccg taaccgcagc ccctataacc agaacctttt tcccctttat ctgcataagt
    11221 actttcctcc gcaatacctt ttactcgtaa cccttcccca gagcccagcc ctaatcctcg
    11281 catcccgcaa cctcgcaccc cgaaacctgt tttacgataa tcccaatatt cctatcaagc
    11341 ttaatattat tgaagatata atgaaaaccg ctactatttt cgattccgcc cagccgctta
    11401 gttcaaaatg atggtgcagg ggcgccattt taaagaacct tttccctccg gtcatcttga
    11461 aataagccac ctgtattatt accgacagcc cttccagaac ataaatcgca cctattatga
    11521 gaatgaataa gggaagcctg gttatgacag caagagaagc tatagctcct cccaaggcta
    11581 atgaacctgt atctcccata aatacctgtg caggatgaga attaaatctt agaaatccaa
    11641 gcagtgcacc tatcatagcc cccgaaaaca ccagcagtcc ggtatttttt gaagtcagtg
    11701 ctattaccga gaaaaatcca agcaccacaa ctgtggttcc ccctgccagg ccgtccaggc
    11761 catctgtaag gtttacgcaa tttaccgtag atatcagtac aaagactgta aatggtatgt
    11821 acaatattcc catatcaata aactttcccg taaaaggtac cagtacctct gttccaacct
    11881 gtgatatccc cgaagcaaca acagccaaaa aaacagaaac cagaagctgc agtattatct
    11941 tctgataagc cttcaggccc agggatcttc tgttttttat cttaagaaaa tcatcaataa
    12001 acccgattat accaaaaagc atagtggtaa taagcaccaa aagcatatct ttatccttgg
    12061 agaaagccag tgacacaatt actgcaggta ctgcaattat tatacctccc attgcagggg
    12121 ttcccgtctt tttcaaatgg ctctgaggcc cgtcatccct gacaatctgc ccgaacttca
    12181 ttctcgtaag cacaggtatg aataccggcc ctgcaaccaa tgccagtaca aaagcagaga
    12241 ttatgcttat gatatatata tcatattcac cggataccaa attgtacatt aaagcttatc
    12301 ccctctcgcg taaatagtca actatgtatt ccattttcat tcccctggag ccttttacaa
    12361 ggacggtatc tcccggcctt attatataat caagggatgc ggcagcagct tttatgtctg
    12421 aaaaggcata tacattgcag ttcattcccg aggccgctgc ccctctttta atatcttcgg
    12481 agaattctcc cacagctatt atggcatctg ctccggagga agcggcatag gctcctgttt
    12541 tacagtgttc ctcagaagaa agattaccca gctcaagcat gtcccccagt atgcatatgc
    12601 ccctgccttt cgacttcatt gtgcgcaata cgtcaattgc cactttcatg gattccggat
    12661 ttgcattata cacatcatta atgagcttta ccccgctgcc gagaactatg atttccatac
    12721 gcatttccga cggtttgaag ctatccaatc ccttttgtat ttcctctgcc ttcatgccaa
    12781 aaagccttgc aacggcaatg gcggacaagg cattatatac attatgtatt cccggaagca
    12841 agactctaaa attcgactgt ccatccaaat ccactgagaa ttctattcca gcttcaccct
    12901 tttcctctat cccataggcg aataaatcat tgcctttttt caggccatac ttaataaccc
    12961 tgaacttatc cgaacagaag tcacggagca tgtcgttatc tccgttaata acagctgtgc
    13021 tgtcactatt aaaatatgaa aatatctcca gctttgctct cagtatgttc tcccttgaac
    13081 ctaacttctc gatatgggta aggcctacat tggaaattac tccaataccg ggccttgcga
    13141 tatccgacag caggctgatt tctccgaggc tgctcattcc catctccaga accgcaatct
    13201 gatggctgta ttgaagcttg agaagcgtca ggggcaaacc gatggcattg tttaaatttc
    13261 cttccgtctt taatacattg aatctttggg tcagcaccga tgctatcatg tccttggttg
    13321 tcgttttccc tgagctgccg gttattgcca caattggtat attgaacttg cttctgtagt
    13381 aagctgcaat gtcctgcaga gctttcagag tgtcttccac cagtatgtac ggtattttgc
    13441 atctctccgg cagcctttgg gttactgcca ataccgcgcc cttttcaatg gccatgtcga
    13501 taaaatcgtg gccgtcaaag ttctcaccct taagcgcaat aaacatatcc ccgggattta
    13561 tttttctgct gtcaatgctt acacccgaga tctcagattc ttcttcaaaa ttcatgagta
    13621 ctccatgtgc agctcttgct atttccccca tacttaaggc ttccatcata cttcctccct
    13681 cagaagctcc tctacaatat ctctctcatc aaagggaata gtcctgtctc tcaatatctg
    13741 ataagtctca tgcccctttc ctgccagcaa aactatatcg tcgggtttcg ccgtggcaag
    13801 agcaaatttt attgcatctc ttctgttttc tatacaaata tagtcacaac ctgtggattt
    13861 tattccagct tctatctgca tgattatatc catgggctct tccgttcttg gattatccga
    13921 agtaattatg cagtaatccg acatccttcc ggcgatattt cccattatgg gcctcttctc
    13981 cttgtccctg tctccgccgc agccgaacag ggttattatt ctgcccctgg catactcttt
    14041 cactgaagcc aggatgttct gaagcccgtc gggagtgtgg gcataatcaa taattaccgt
    14101 ataaggcgta tttgtcttta taatctccga cctgcccgga acattttcga ctctgctcag
    14161 tccttcccgg attacctcca tgtccaaccc ctccgcatat gcagccgagg cagcagccaa
    14221 ggcattgtat acgctgaact ttccgggtat actgatgttc agttctgcct catattcggg
    14281 agtaataaca gtaaagccca ctccttttga agttatatca atattttttg ccattatgtc
    14341 cgccgcattg tcaataccgt aggtaatcag ctttttcccg tttttcttca actcggcggc
    14401 tattattttc ccatgctcat catcaatgtt tattatactt gtaccgctta ccatataaaa
    14461 gagctttttc tttgcatttc cgtaattttc aatggtttta tgaaaatcca aatgatcctg
    14521 ggtcagattt gtgaatattc ctattctgaa attgctgcct tccacccttc caagctccaa
    14581 tgaatgggag gatacctcca ttacagcata atcgacatcg ctgtccgcca tctccctgaa
    14641 aaggcactgg agatccaagg cttcaggcgt agtccttgaa gaaatatata tcttatcgcc
    14701 gatctgggtt gaaattgtac ccagaagccc aattttttgc ttgtttgctt ccattattga
    14761 ttttatcaga tacgtggtgg tggtttttcc gttggttccc gttataccta tcagcttaag
    14821 cttttgtgag ggatttccgt agaaattgga tgcaagcatc ggcataacag cccttgagtt
    14881 ttccaccttt accaccgccg ctcccggaac ttcgacgtcc ttctccacaa taaaagcggc
    14941 agcccccttc tgcagcgcat catttatata actgtgcccg tcatacttat agccgctgac
    15001 acagataaac aggtcaccct tctttgcctt tctggaatca tatattattc cgtttatttc
    15061 tatattcaaa tcccctttga aagatacagt atcaacacct gttaacaatt cctccagctt
    15121 catatttatc ctccttcata tacagctatt gttgacatgt tttggcatct atatgctgaa
    15181 tgcgtaatca tcagttgtaa atcactttat tattatccat cacattttac aaaaaggcaa
    15241 ccaaaattaa agggtttctt taatttcgtt gcctggcaat aaaacctcag atatatgctt
    15301 aatacataat gctattactt cattgtacgg gtttagcatt caaaattaca atagaccctt
    15361 cattaatatc tgctccgggc ttcggagtct gatcaactat tgttgattcc atgttatgct
    15421 caaattctgc ttccacactg tatttgagct tgctcttttc aagttccttt atggcattct
    15481 taagggccat gtttcttact tccggcacct ttacaagggg tctctgcata agctcctttt
    15541 cttctgcagt atattggggt ttgacatcca tgtatctcag agtgtcatat attatgctcc
    15601 tggctacagg tgctgcaatt acaccgccat agtactggcc tgctccgggc tcgtctatga
    15661 ccaccagcac agatactttg gggtcgttgg caggagcaaa accgcagaag gatgaaacaa
    15721 atttgcctga tacatatatt cccccttcgg ctttttgagc tgtaccggtc ttccctgcaa
    15781 ccttatatcc cggaatataa gaggatgcag tgggcggtat cgcaaattcc agaatctgcc
    15841 tcattgtcgc cgaggtttcc cttgatatta cctgtctgac tgcccgaggc tggaattcat
    15901 ggactgtcgt ttcttcacca tcaatgactt tcttggcaat tctgggctcc ataagcatac
    15961 cgtcattggc aaccgcagag aaggcggtta tcaattgaat aggcgtaacg gatattcctt
    16021 gtccgaagga attatttgca agctcaaccg ggccgacccc gcccaattct cttacaatac
    16081 ctcttgcttc tcccggaagc ttgatatttg ttaccgaacc aaagccaaag gcttttatgt
    16141 atttatacag cttttccttt cccattcttt gccctacttc tacaaataca ggattgcagg
    16201 aattcttgac agcctctgca aagctttggt tcccgtgagg cttgtagctt ctccagcatt
    16261 tcatcttctt ccctgccacc gttatgtgcc ctatgcagtt gaaatgatcc tccggtttaa
    16321 caacgttttc ttccaatcct gcggaagagg ttattacctt gaaagtggaa ccgggttcat
    16381 aggaatccga aatagcagga tttctccata tcctataaag tacatccagc tgctctttct
    16441 cgctcatggt gctccattta tccgcaaggt cctccggaac cttccgggga tcattgttat
    16501 caaaatcggg tttggaagtc atagcaagaa tgtcccctgt cttaggatcc atcactattg
    16561 ccgtaacccg ctttgcctta ttctcaataa gtgcgttctc aacaaccttc tcagcgaaat
    16621 gctgtattac ttcatcaatt gtcagcacaa gattcaggcc gtcctgaggc tcgtaatact
    16681 tggtgtcgct tcccggaagc atctctccgt gggcatctgt cattacaata ttccttccgg
    16741 gaaatccgtt caggtacttt tcatatgtaa gctctacccc atcctgtccc tggttgtcag
    16801 ggttggtaaa acccaataca taggaagcga aattgctttg agggtaaaat ctcttgctgt
    16861 cgtcattaaa tacaattccc tgcagttcct tttcccttaa aagcttaacc ttttcggcgt
    16921 ccaccttttt cttgatgagt accgtgtcct tattggcagt taccttttga gccaaagaag
    16981 ccttatccat ccccagaatt tccgacaggg catttgtaac cgcatcttta tccttgacca
    17041 tggcaggcct tacatatatt tcatatgctg ttgcgctgac agccagcttt ttgaagtttc
    17101 tatcgtatat tattcccctt ttggacctta tcgggatatc ccttgtccac tgttccaccg
    17161 ctttctgctg gtattcggca ctgtttacta tctgtataaa agccagtctt cccaggagtg
    17221 ccgcgtaaat aacaaaaaaa gcaaagaaga taattacaat tcttcttttt atagtcactt
    17281 taatgctgga caaattaata caccaccttc agaaacaacc tcaagctaat ctaatacttt
    17341 taggatatta tttatcacag ctttggtata tgctatgtat ttattctcct gtaaatcgtc
    17401 aatgttgtag aagtcacgag cctcattgcc ggcaggctgt gccggtatct cgacatttac
    17461 ataagcaatc tggttttgaa tatcaggata ctgcattccc agctcttcaa ttgccttttt
    17521 ttcaagagtc tcaaggctga ttgacttcat aagctgcaca ttcaaatcag tattttcctt
    17581 aaccagagca ctgtattcct tttccaggtt ttttatattg tagtttatct ccgccatata
    17641 agcatagcgt gatataataa aaatgctcag cccaaaaaca aacaatatac tcatcagatg
    17701 agtcttgtgt ttttctttct tatgaacctt ctttcggggg ctcgcagcta tttcccggac
    17761 ttgctccatt tcaagctgct catatctgct gtaatcatat gccgtattgt ctgctcttaa
    17821 cattattatt cccccctaac cccttttaga acttactctt tcggccgctc taagcttagc
    17881 gcttttcgac ctggtattag tcttcagttc ctccactccg gctgtcacgg gtttttttgt
    17941 aatcactctg agaagaggct cttttccgca ggcacatacc ggcagctgag gcgggcatgt
    18001 gcacggcttt tccatattgt taaatatctt ttttactatc ctgtcctcaa gggaatgaaa
    18061 ggttattaca accagccttc ctcccggctt caaaagcctt gctgcgtttg taactgcctc
    18121 ttccaaaacc tgaagctcgt cattcacagc tattctcaag gcctgaaatg tccttttggc
    18181 gggatgtccc ccatccctgc gggccgccgc cggaattgcc ctttgtataa tatccgccag
    18241 cttaaaggtt gtatctatcc tgctgttttt tctttcttcc gttataaact tcgcaatcct
    18301 gaccgcccat ttttcttccc cataatccct gagtatacgg ataagctcct gttccgaact
    18361 attattcact atatccgaag cattaaggcc gttttccgta tccatcctca tatccagggg
    18421 gccgtcctgc atgtagctga agcctctttc cttctcatcc aactggtggg aggatacccc
    18481 tatgtcaagg agtattccgt caacttcctt aaatcctttt tgcagggcta tggtttttat
    18541 attcctgaag ttgtcacgga caattattac attgtcctta tatgcttcaa ggcgcctcgt
    18601 tgcagcatca attgcattgc tgtcctgatc aatgccgatc agcacccctc cgggaactat
    18661 cctttttgct atttccgaag agtgccccgc ccctccaagg gtaccgtcaa tatatatccc
    18721 tcccggcttg ggatcaaggt attccaatgt ttcattcaac agcacgggaa tatgttcgaa
    18781 atccaatact gccatctgct tcacatgcct tttcgctata ttccgagctg tgacattttt
    18841 tcagccacat cctcatagct aatatttgca tcgttgttaa atttattcca ttcttccttg
    18901 ctccatattt ctattcgggt tgagacacca ataattacaa gctctttatc tatattggca
    18961 tgctccttaa ggtaatttgg tatcaccatc ctgccctgct tgtccaggtc acattcgcag
    19021 gcccccgcaa aaaagaatct tacaaaggct cttgcatctt tgtttgtcat aggcaggctc
    19081 ttaagcttat cctccactat gctccaatcc cgggacgagt atatgaaaag gcagttgtca
    19141 agacccttgg tcataacaaa gctgtcccca agcttttccc tgaattttga aggcatgatg
    19201 actctgttct tcgggtccag ggtatgattg tattcgccta taaacatata ctcaccacct
    19261 ttataacaag atttcaataa gagttttgca tacttgcaaa aacctacccg agcgtttcaa
    19321 cggggcagag atatgttcat cttctgctga acaacgaaag cgttacctgc cgcttgtata
    19381 gtattggaga aacttattgc cccgttatat tcacattata ccactttgct ccactttagt
    19441 ccacttcttg acatattcta tacaaaaata aaaattcctc ccttttttaa aaaaatagga
    19501 gaaattttaa aaaaatgatg caaaagacct atgcttttgc atcgttatta ttcttcagca
    19561 atatcctcag tgtcctcccc ggccttatcc ttattctttt t
//
LOCUS       DAOQUR010000042        18017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3572_length_18017_cov_10.352021, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000042 DAOQUR010000000
VERSION     DAOQUR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18017
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_18017_cov_10.352021"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..662
                     /locus_tag="PKI50_08975"
     CDS             51..662
                     /locus_tag="PKI50_08975"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNT03872.1"
                     /translation="MNKLERSFYLRDPLIVAEDLLGKHLVHETRDFRVVGKIVETEAY
                     AGPEDRACHAYGLRRTKRNEIMYHEGGFSYVYFIYGMYYCFNVVTEQKDNPSAVLVRA
                     VEPIDGIGHMARLRYGMDLLSLSASQRSVMCNGPGKLCMSMGIGKAENGLDLCGSTLY
                     ICDAHNEPFSVKSSPRINIDYAGEYKDKPWRFYIEGNKHVSKG"
     gene            complement(683..1345)
                     /locus_tag="PKI50_08980"
     CDS             complement(683..1345)
                     /locus_tag="PKI50_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNT03873.1"
                     /translation="MPSYYELFVRLALACFLGGVIGYEREKTRHPAGFRTHILVCVGS
                     ALAMLCNIYLFEEYKGIANIDPGRFGAQVISGIGFLGAGTILKEGATVKGLTTAASLW
                     SVACIGISVGLGFYLGSLLTTAFVLVALVVFSRFEVLISGGKKNTTLKIRSEDKPGQL
                     GKIGTHLGSFNLKICDIAIEVEDEASVMVKIKVNRAKENISPEVLDSLSKIEGILKVE
                     VC"
     gene            1596..2432
                     /locus_tag="PKI50_08985"
     CDS             1596..2432
                     /locus_tag="PKI50_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNT03874.1"
                     /translation="MISEAEEKYMVINGKVMPVSGAGDILAGSSRTIYEVIKVVSGIP
                     LFLEKHMERLEASARLINYSVTNIMNSIRESITELIKANNSPEKNIKIIVYNMGNPVP
                     DFMAYFIRSSYPTPEQYETGVHGILLREERNNPNAKVVNSGYKERVAAALAEANAYEA
                     LLVNREGEITEGSRSNIFFVRNGKVLTAPKGNVLIGITREYVFDLCKNLGIEIIEEPI
                     SVSMLGEMDGAFMTGTSPKILPISTIDDMSFSSPKNPVIKALISSYNDIVEKYIKEKT
                     IG"
     gene            complement(2435..>3415)
                     /locus_tag="PKI50_08990"
     CDS             complement(2435..>3415)
                     /locus_tag="PKI50_08990"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HNT03875.1"
                     /translation="ARGGIYEVLKHAEDLAVKHGLIRIDDDYSRLGSPEFKEFFGKFS
                     LAVGSTGNLGLSIGIMGSALGFNVTVHMSADAKEWKKSLLRGRGVNVIEYSSDYSKAV
                     EEGRRQSQQDPNSYFVDDEHSKSLFLGYSAAAFRLKKQLDALGTKVDESNPLFVYVPC
                     GVGGAPGGICFGLKHVFGDNAHVFFAEPTHSPCMLLGLATGENEKLSVQDFGIDNITD
                     ADGLAVGRPSGFVGNMVGRLLSGIYTVEDNELYKLLAMMKDEENIKIEPSATPGLLGP
                     LQAEAYINANLPEVSMKNATHIAWATGGLFVPEAMMQEFYERGKKLLGEL"
     assembly_gap    3416..3515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3667..4110
                     /locus_tag="PKI50_08995"
     CDS             3667..4110
                     /locus_tag="PKI50_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HNT03876.1"
                     /translation="MPLATREIFARMIKCEAGGEGDNGMKAVATVIMNRVHVPNGEYQ
                     RIGQGDLRRILFQPGQFDCASDTLGGAYNAQNIYNMRPDQIHYDIADWALSGNKMLTV
                     AYCLWYFNPFTQTCIQTFPRNGSGTFANRIGEHCFYNPTRLYYTT"
     gene            4187..4672
                     /locus_tag="PKI50_09000"
     CDS             4187..4672
                     /locus_tag="PKI50_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03877.1"
                     /translation="MYSNSSYSFSQTPASSGNQSQFSQAPEMGTTFGYPMAPQTFPIQ
                     QQMGAQQMGGQQMGQQMPQMPQMPPPTMQMPVTGAAPVDFDMLPTTPVLDIEYTQGYL
                     KTQIGKRVKVEFLIGTNMLIDREGTLVDVGISYIIINEVETDDLLLCDIYSIKFVRFY
                     F"
     gene            complement(4798..5973)
                     /locus_tag="PKI50_09005"
     CDS             complement(4798..5973)
                     /locus_tag="PKI50_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT03878.1"
                     /translation="MKTDQDRIKSLTEYVQGLLDKENGRELYFKYRTYIERVTPQEVF
                     EIFHLILQKGIKPKEILTVLDKAINVFYKSLSNYTWKWPDKDSFMDYLMRENRALEEK
                     LEGIKEIIKDMSSEAGKKELILRMHELRKFNDHYLKKENILFPYLEKKMKKFDGLAIM
                     WALHDEARAQLKKVIECLEAEGCIEGEFNSELGKLFFDMYGLVQKEELILFPAASEII
                     TDDEWLEMQRQSMGYEFPFIERPEPADNEYIPGIKEGCRFETETGALDFNEILAIFDA
                     LPVDLTFVDENNKVKFFTKPKDRIFPRSPAVIGRDVEKCHPPESVHVVNEILDAFKEG
                     RKDNAVFWIKVKGRMILIQYFAVRDSVGRYLGTLEASQDITDIIKLEGERRLLQFNK"
     gene            complement(5982..6794)
                     /locus_tag="PKI50_09010"
     CDS             complement(5982..6794)
                     /locus_tag="PKI50_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035109.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNT03879.1"
                     /translation="MSSIGIYYFSGTGNSLYVAKELQKRIEGAKLIPVISRLDREAVE
                     TDAETIGFVFPVYFSAIPVPVKNFIKKLDLKSAKYIFAAATRIGTSHSAFMAIDKILK
                     SKGQRLDSYFSLNMPGNDPKFNYKVPTEEEIARLEAVVQKRLDSIQSIIKNKEISREK
                     DVDCTIHIPFVGILSSLAALIDISKDNLYCDSKCTGCGVCEKVCLAHKIKMVDGKPEW
                     QDNAKCYHCAACLNYCPVQSAQIKSQTEKNGRYSHPYAAVEDIAMQKNFERR"
     gene            complement(6810..7211)
                     /locus_tag="PKI50_09015"
     CDS             complement(6810..7211)
                     /locus_tag="PKI50_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNT03880.1"
                     /translation="MKSRRRVVLAVVIALAAIVTAVFGFKSYTEASLVKLAETSIPDV
                     DLSKIADGVYKGSYKVFPVEAEVRVTIENHKIVAIELVKHFNGQGAAAEVIPDRVTEA
                     QSLGVDIVSGATYSSKVILKAIENALQSANR"
     gene            complement(7208..7708)
                     /locus_tag="PKI50_09020"
     CDS             complement(7208..7708)
                     /locus_tag="PKI50_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNT03881.1"
                     /translation="MKIGIIVHSKTGNTYSVAEKLMKTLAAKGHIASIEQVEACDNGQ
                     MDAIKVQLKSKPDINPYDALIFGAPVHGFSPSVVMEAYLTQLMAIPDKKAACYVTEFF
                     PLPQMGGNQAIERMKEICSSKGFDVIGTGVVNWSNIHRKGMIDNLAEKFSGLFMSLSE
                     CKEGNK"
     gene            complement(7705..8319)
                     /locus_tag="PKI50_09025"
     CDS             complement(7705..8319)
                     /locus_tag="PKI50_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT03882.1"
                     /translation="MKEKTAEDKKILKKQRIRTYFLEAAREIILNDGVESVTVRKVAD
                     MAGYSYATIYNYFSDLNQLLWEVKKLMINDIVEALYKKMAGMTKDVDGIKKCFKMYVE
                     YFIVNPNVFRFFYFHHLSKPAGETGENEEEPDFDAMWEETFKEFVISGKIRAEDIEVL
                     ARTMIYAIHGLITLYFSEDVDYKGEDVYSELDKVIEYILGRGQI"
     gene            complement(8519..8677)
                     /locus_tag="PKI50_09030"
     CDS             complement(8519..8677)
                     /locus_tag="PKI50_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03883.1"
                     /translation="MKYSPKPTFEFYKVYRKRINQIKKEVDASLQVDNGAFCGFLMMS
                     PESSEDTE"
     gene            8822..9346
                     /locus_tag="PKI50_09035"
     CDS             8822..9346
                     /locus_tag="PKI50_09035"
                     /inference="COORDINATES: protein motif:HMM:NF039476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HNT03884.1"
                     /translation="MDLKRWQIIGAVFALIMGALLHFTYEWSGENPVVGIFSTVNEST
                     WEHLKLLFVPVALFAALEYIGYGKQIPNFVPVKVLSILLGMITIIIAYYTYTHIVGQN
                     YLWVDIGIFILGVLAAYWFSYSLLQTEFLASECAVCLGWAGLLLLAFSFAFFTFCPPH
                     VELFLDPVTGTYGR"
     gene            complement(9406..9987)
                     /locus_tag="PKI50_09040"
     CDS             complement(9406..9987)
                     /locus_tag="PKI50_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT03885.1"
                     /translation="MDCSKVGKLIFGLRKEKGMTQKQLADAMNISDKTISKWERGLGC
                     PDVSLLRELSEVLNADIEKILLGDLEPNNADGGNMKKIKFYTCPNCGNTLTSTGESDV
                     SCCGRKLVPLIPKPAEGTHKLTVEEIEEDFYITFAHEMSKTHYINFVAYVAYDRVLFV
                     KLYPEQGSEVRFPKMYRGKIYLCCNQHGLWVND"
     gene            complement(10339..11490)
                     /gene="nagA"
                     /locus_tag="PKI50_09045"
     CDS             complement(10339..11490)
                     /gene="nagA"
                     /locus_tag="PKI50_09045"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017531532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNT03886.1"
                     /translation="MKAIVNARIIKDNSIFDGYTVLFEEKILRLMEGNALKGLEPEEV
                     FDAAGRYLSPGFIDVHIHGCIGNDTMDDNESSLRNISKAIAATGVTAILPTTMTMGFP
                     MIEKSINRIRTLMDDAPGASILGCHLEGPFISEEYKGAQDGKYIRRPDFKTIEGLSDV
                     IRIVTIAPEEEGSEEFIQSCVSQGIVVAIGHTNASYEQAMRAIQKGASHITHIFNAMT
                     PLNHREPGAVGAAMDSSVVCELIADNVHVNPAVQRILLKVKGVEGIILVTDAMKACLL
                     EDGEYELGGQRVVVKNEVARLPAGNLAGSVLTLDKALRNFTANTGIGLIDAIKTVTEN
                     PARVLKVNDRKGSIDIGKDADFVLFDDSFNIHATFVKGKKVYERVVKWE"
     gene            complement(11491..11778)
                     /locus_tag="PKI50_09050"
     CDS             complement(11491..11778)
                     /locus_tag="PKI50_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03887.1"
                     /translation="MRINANTEKIDKLSTIEMVTLFNNEDKKVAVIDASECPPTFGID
                     DNVIIGVISGRIEAVSGWKEEEALKAFEKAGGNARAAILSLVNDSSKIEGD"
     gene            complement(11833..12408)
                     /locus_tag="PKI50_09055"
                     /pseudo
     CDS             complement(11833..12408)
                     /locus_tag="PKI50_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(12577..12960)
                     /locus_tag="PKI50_09060"
     CDS             complement(12577..12960)
                     /locus_tag="PKI50_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03888.1"
                     /translation="MKKYLVIILVLTAALSLAACQATDVVGKFAVTSFEALLNKIPEN
                     ITSDEVNNGWALTSPTGESFIWGKDFSSEGTPDVMLEFDSQPFIDAGADPNKVEGWVF
                     AKVPVMDEQGKKIEVEKLLKPFDLK"
     gene            complement(12980..13300)
                     /locus_tag="PKI50_09065"
     CDS             complement(12980..13300)
                     /locus_tag="PKI50_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNT03889.1"
                     /translation="MSSKGGIPLDCNCDHNVTVRTEKEKKDIISRLNRIEGQVRGIKG
                     MIEKDVYCDDVLNQISSVNSAMNSVGKLILKNHMKSCLIKRIQAGELDVVDELLYTIE
                     KMLK"
     gene            complement(13301..13516)
                     /locus_tag="PKI50_09070"
     CDS             complement(13301..13516)
                     /locus_tag="PKI50_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNT03890.1"
                     /translation="MKKQINIEGMSCGHCVKHVEGALGEVPGVIKVDVSLEGKYAIVE
                     LEKEVPDSVLKNAVDEAGYDAGAVKVL"
     gene            complement(13615..16077)
                     /locus_tag="PKI50_09075"
     CDS             complement(13615..16077)
                     /locus_tag="PKI50_09075"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNT03891.1"
                     /translation="MMRSDIFRINGMTCAACAKAVERAVKKLDGVSDATVNLATEKLS
                     LTYDEAKLKPSDIAEAVSKAGYEALQEKNTKEVSIPIEGMTCAACAKSIERAVGKLSG
                     VEAVSVNFATEKANVKYDPAELRLSEIKQAIVKAGYKALEIESKDKVDEDKKRKDREI
                     RILWTKFIVSAVFAAPLLYIAMGHMLGLPLPKALHPMDFPLRFALVQILLTVPVVTAG
                     YRFYTVGFKAILHRSPNMDSLIAMGTSAAVLYSLYSTYQISIGHFMYAEDLYFETAGV
                     IITLILLGKSLEAVSKGRTSEAIKKLMGLAPKTAIVMQAGEEIEIPIEEVEVGDIIAV
                     KPGSKIPVDGEVVEGLTAIDESMLTGESIPIEKKPGDKVFAASINKTGSIRFKATKVG
                     SDTALAQIIKLVEDAQGSKAPIAQMADIVSGYFVPIVFVIATLSALAWYFIGGETPVF
                     ALTVFIAVLVIACPCALGLATPTAIMVGTGKGAEYGVLIKGGEALETTHKIKTIVFDK
                     TGTITEGRPEVTDIVTTEIIKKDKLLQIAASAEKGSEHPLGEAIVRGAEKENLEDLKV
                     DKFNAIPGQGIEVEIEGKNILLGNRKLMTERNISLSGLETESDRLASEGKTPMYAAIG
                     NQLAGIIAVADIVKESSAKAINKLHEMGIEVVMITGDNRKTAEAIAKQVGIDWVLAEV
                     LPQDKANEVKKLQSEGKKVAMVGDGINDAPALAQADIGIAIGSGTDVAMESADIVLMR
                     SDLMDVPTAIQLSKSTIRNIKQNLFWAFGYNVAGIPVAAGLLHVFGGPLLNPILAAAA
                     MSLSSVSVLTNALRLKGFKPYK"
     gene            16246..>18017
                     /locus_tag="PKI50_09080"
     CDS             16246..>18017
                     /locus_tag="PKI50_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT03892.1"
                     /translation="MSIKKERIKIFEMTCTSCENRVEKAVRQLEGVIYAKASYSGQYM
                     EIEYDDDLCGMNNIKDAVKNAGYSTEGSKDCKFMGILIIAVGVVLLGLKTGGFDMEAK
                     LTNASYAVLFIVGVLTSLHCIGMCGGIMLSQSLSKESKNRFEAIQPALLYNIGRVISY
                     TILGGLIGALGSVFTLSIAAKAAVQIFAGAFMIIMGLNMAGFSAFRKFQIKLPQFACK
                     AKSKSGSPLIVGLLNGLMPCGPLQTMQLFALGTGSAAKGALAMFVFSIGTVPLMLTFG
                     ALSGLLSKGYTKKILKFSGVLIIVLGLIMGNRGLALAGININPITAFASNSAGSSNPY
                     VAKATLQDGIQIINMTADNNGYAPNAFYVQKGIPVRWIIDGKELNSCNNAIVARGLNL
                     ERKIKKGENIIAFTPGDKDINFSCWMGMISGVIKVVDNLDSVDTSKADPSLPPPSTGP
                     SCCAVPIEEDEPSRPSIYGDDISKVPTEKLVNKAMADGKNQSVIFKGIGYELQPLIAV
                     AGSGGKIRLTLDLNDFDNAEGEYLIIDAATGKDVTAFTAEKGINEIEFSPKNVDGYAI
                     IKGNSILGIIEVVDNLEAADIKEI"
BASE COUNT         4879 a         4346 c         3359 g         5333 t
ORIGIN      
        1 gcgcattatt cttcaaaaac gaaacataaa aaaatataga cggaggtact atgaataagc
       61 ttgaaaggag cttctatctt agagatccat tgatagtagc agaggatctg ctggggaagc
      121 atctggtgca tgaaacccgg gattttagag ttgtcggaaa gattgtagaa actgaagcat
      181 acgcaggccc tgaagacaga gcctgccatg cctatggcct gagaagaacc aaacggaatg
      241 agataatgta tcatgaagga ggcttctcct atgtatattt catttacgga atgtactact
      301 gcttcaatgt ggtcacagaa cagaaggata atccctcagc agtactggtg agggcagttg
      361 agccgattga cggaatcggg catatggcca gattaagata cggcatggat ctgttatcac
      421 tgtctgcaag tcagagatcc gtaatgtgca acgggccggg aaagctctgt atgagcatgg
      481 gtataggaaa ggctgaaaac ggacttgact tgtgcggcag cacactttat atatgcgatg
      541 cacataatga acctttttct gtaaagtcct ccccgagaat caatatagac tatgcagggg
      601 aatataagga taagccttgg agattctaca tcgaaggaaa caaacatgta tcaaagggat
      661 gatttaataa atcccttcaa tatcagcaaa cttcaacctt caatattccc tcaattttgg
      721 acagggaatc taaaacctca gggctaatat tctctttggc cctatttacc tttatcttta
      781 ccatgaccga agcttcatcc tccacttcta tggctatgtc acatattttc agattaaatg
      841 aacccaaatg ggtgccaatt ttccccaact gccccggttt atcctctgat cttattttca
      901 gggttgtatt tttcttgccc ccggatataa gtacttcaaa tcttgaaaag accacaagag
      961 caacgagtac aaaagcggtg gtaagaagcg agcctaaata aaaacccagt cctacggata
     1021 ttcctataca agcaaccgac caaaggctgg ctgcagttgt cagccccttg acagtcgcac
     1081 cttctttcaa tattgtgcct gcccccagga atcctatgcc gcttattacc tgtgcgccga
     1141 aacgtcccgg atcaatattg gcaatacctt tatactcctc aaataaataa atattgcaca
     1201 acatggccag tgcagatccc acgcatacca atatatgcgt cctgaagccc gcaggatgcc
     1261 tggtcttttc cctttcatag cctatgaccc cgccgagaaa gcaggcaaga gcaagccgga
     1321 caaacaattc ataataactt ggcatgaaat tccacctcct tttatgatat atatcggctt
     1381 atcgtgccaa tattcctaac ccgtatttaa tatatattcc gtgaaaaagt tttatcaccc
     1441 caaaagcagc agtacttttt attaaagtaa tgtgtttccc ggcgcattaa tttcggaaaa
     1501 tgtttgacac taagcatata cctcttgaaa tataatacac ataatgctag atttctatta
     1561 tagaacaatt aattacagag acaggagaca gattgatgat cagtgaagca gaagaaaaat
     1621 atatggttat aaacggcaaa gtaatgcctg tttcaggggc aggggatatt ttggcaggct
     1681 cttcccgaac aatctatgag gtaataaaag tagtgtcagg aattcctctt ttcctggaaa
     1741 aacatatgga aaggctggag gcttcagcca ggcttatcaa ctattcggta accaatatca
     1801 tgaatagtat acgggaaagt ataacggagc ttatcaaagc caataacagt cctgaaaaaa
     1861 atataaaaat aattgtttat aatatgggaa accctgtgcc ggactttatg gcgtatttca
     1921 tccgcagcag ctacccgacc cctgaacaat acgaaacagg agtacatgga attcttctca
     1981 gggaagaaag gaacaatccc aatgccaagg tggtaaattc aggttacaag gaaagggttg
     2041 cagctgcttt agctgaagca aacgcctatg aagcactgct ggtgaacagg gaaggtgaga
     2101 ttaccgaagg aagccgctcc aatatatttt ttgtcaggaa cggtaaggta ttgaccgccc
     2161 caaaagggaa tgtactgata ggtataacca gggaatacgt atttgatctg tgcaagaacc
     2221 tgggaataga aataatcgaa gaacccatat cagtttccat gcttggggaa atggacggtg
     2281 cttttatgac cggcacctca ccaaaaatac ttcccataag cacaattgac gatatgagct
     2341 tcagttcccc caaaaaccct gtaatcaaag cgcttataag tagctataat gatattgtgg
     2401 agaagtatat aaaagagaag acaattggat gagcttatag ctcgcccaat agttttttgc
     2461 ctctttcata gaactcctgc atcatagcct cgggcacaaa caaccctcct gtagcccatg
     2521 caatatgagt cgcatttttc atgctgacct caggcaggtt ggcattaatg taagcctccg
     2581 cctgcagggg gcccagcagc cccggcgtag ccgaaggctc aatcttgata ttctcctcat
     2641 ccttcatcat tgccagcagc ttataaagct cattatcctc aacagtataa ataccgctta
     2701 aaagcctccc caccatattg cctacaaagc ctgaaggcct cccaactgca agtccgtcgg
     2761 catcggttat gttatctata ccgaaatcct gcaccgacag tttttcattt tcgccggtag
     2821 ccagaccgag aagcatacat ggggaatgag tcggctccgc aaaaaataca tgtgcattat
     2881 ctccgaagac atgcttcagg ccaaagcata ttccacccgg tgcgcctcca accccgcagg
     2941 gcacatatac aaacaaggga ttgctctcat ctacctttgt tcccaaagca tcaagctgtt
     3001 tcttcaaacg aaaagccgcc gcgctgtatc ccaggaacaa gctcttggaa tgctcatcat
     3061 ccacaaagta actgttcgga tcctgctgtg attgccttct cccttcctca accgccttac
     3121 tgtaatcgga agagtattct atgacattga ctcccctgcc ccttagaaga ctctttttcc
     3181 attcctttgc atcagcagac atatgtacgg taacattaaa acccaaggca gaacccatta
     3241 ttcctatact cagccccaag tttcccgttg agcctacagc cagggaaaat ttaccgaaga
     3301 attccttgaa ctccggcgac cccagccttg aatagtcatc gtcaatacgt atcaaaccgt
     3361 gcttgacagc caaatcctcc gcatgcttca ggacctcgta aatgccgccc cttgcnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagaa aatttcacat ttacaaaaat
     3541 tttcttaccg ctgcatattt aacaagacga aatctgtttc accctttata aattctttgc
     3601 tgttacagtg tcaaataacc cgctgataca taaaatgata atatcactgt atgcgaggtg
     3661 aattttatgc cgcttgctac cagagaaata tttgcccgga tgattaagtg tgaagccggt
     3721 ggcgaagggg acaacggtat gaaggctgta gccaccgtta ttatgaatag agttcatgta
     3781 cccaacgggg aatatcaaag aatcggccaa ggggatttaa gaaggatact gttccagcca
     3841 ggccagtttg actgtgcttc agatactctc ggaggagcat ataatgcaca gaatatatat
     3901 aacatgcggc ctgaccaaat tcactatgat atagccgatt gggctctgtc gggcaataag
     3961 atgcttacag tggcctattg cctttggtac ttcaatcctt ttactcagac atgtatccag
     4021 acttttccga gaaacggctc aggaacattt gccaatcgaa taggagagca ctgcttctat
     4081 aatcctacaa ggctttacta tactacgtag aaaatccgtc catgcgcgct tcgcgcggca
     4141 aagtaatgca aggacattga aatattatct atgaaaggag ataactatgt acagcaatag
     4201 ttcatacagt tttagtcaga cacctgcaag cagcggtaac cagtcccagt tcagccaagc
     4261 tcccgaaatg ggcaccacct ttggctatcc aatggcgccc cagacatttc cgatacagca
     4321 gcaaatgggg gcacaacaga tgggcgggca gcagatggga cagcaaatgc ctcagatgcc
     4381 tcaaatgccg cctccaacaa tgcagatgcc tgtgacaggt gccgctccgg tagattttga
     4441 catgctgccg acaacaccgg tacttgatat cgagtataca cagggctatc tcaagactca
     4501 gataggcaaa cgggtaaaag tggaattcct tatcggaacc aacatgctta tagaccgtga
     4561 aggaacactt gttgatgtag gtataagcta tatcataata aacgaagttg agacagacga
     4621 cctgctgctt tgcgatattt attcgataaa attcgtacgt ttctatttct aatatggcag
     4681 gtgccgcata ggaagaatta tgcgatgcga ggacacgggg aggacatggg gacgtttctc
     4741 ctgtcccgct gaaagcggga caggagaaac gtccccatgt ccccaccccc gtgtcctcta
     4801 cttgttaaat tgcagcagcc ttctttcccc ttcaagcttt ataatgtccg ttatatcctg
     4861 gctggcttcc agagtcccca ggtatctgcc cactgaatcc cttacggcaa aatactgtat
     4921 cagtatcatc ctgcccttaa cctttatcca gaatacggca ttatcctttc tgccttcctt
     4981 aaaggcgtca agtatttcat tgactacatg gacactttcc ggaggatggc atttttccac
     5041 atcccttcct attaccgcag gggaacgggg gaaaattcta tccttgggct ttgtaaagaa
     5101 ttttacttta ttattctcat cgacaaatgt cagatccaca ggcagtgcat cgaatatcgc
     5161 aagaatttca ttaaaatcca aagctcctgt ttccgtttca aagcggcagc cttccttaat
     5221 gcccggtata tactcgttgt ctgccggttc aggcctttca atgaacggga actcatatcc
     5281 catactctgc ctttgcattt caagccattc atcatcggta ataatctcac tggctgccgg
     5341 gaaaaggata agctcttcct tctgtaccag gccgtacatg tcaaagaaca gctttcccag
     5401 ctctgagtta aactcccctt ctatgcagcc ttcagcctca aggcattcaa tgaccttttt
     5461 tagctgtgcc ctggcctcat cgtgcaacgc ccacatgata gcaaggccgt caaacttctt
     5521 catcttcttt tccaggtagg ggaacagtat attttctttt ttgagatagt ggtcattaaa
     5581 cttccggagt tcatgcatcc ggagtatcag ttctttcttt ccggcctccg aagacatatc
     5641 cttgatgatt tccttaatgc cctcaagttt ttcctccaga gcgcgatttt cccgcatcaa
     5701 ataatccata aagctgtctt tgtcgggcca tttccacgta taattcgaca ggcttttata
     5761 aaacacattt atcgctttat ccagaacagt caggatttcc ttcggcttta tacccttctg
     5821 cagaataagg tggaaaatct caaaaacctc ctggggagta accctttcaa tataagtcct
     5881 gtacttaaag tatagctccc ttccattctc cttatccaaa agcccctgta cgtattcagt
     5941 caaactctta attctatcct gatccgtttt cataaaagta ctcatctcct ttcaaaattt
     6001 ttttgcattg caatatcttc taccgctgca tacggatgcg aataccttcc gtttttttca
     6061 gtttgcgatt ttatctgggc agactgcaca gggcaatagt tgagacaggc agcacaatga
     6121 taacatttcg cattatcctg ccattcaggc tttccatcaa ccattttaat cttatgcgca
     6181 aggcaaacct tttcacatac gccgcatcct gtacatttcg aatcgcagta caaattgtct
     6241 ttggatatat ctataagagc cgccagggat gaaaggatcc ccacaaaggg gatatggatt
     6301 gtgcagtcaa catccttttc cctgctaatt tctttatttt ttattatgct ctgaatagaa
     6361 tcaagccttt tttgaacaac tgcttccaat cttgctattt cttcttctgt aggaacctta
     6421 taattaaatt tcggatcatt tccgggcata ttgagggaaa aatatgaatc caggcgttgg
     6481 cctttgcttt tcaatatttt atctatggcc ataaaagcac tgtgtgaagt tccgattctt
     6541 gttgctgcag caaaaatata ttttgccgat ttcaaatcca gttttttgat aaaattcttc
     6601 accggtaccg ggattgccga aaaatatacc gggaatacaa accctattgt ctctgcatca
     6661 gtttcaactg cttctctatc caaccggctt ataaccggta taagctttgc tccctcaatc
     6721 cgcttttgca attcttttgc tacatacagt gaattgccgg taccggaaaa atagtagatt
     6781 cctatactac tcattataat tgctcctttt tatctgttcg cactttgcag tgcgttttct
     6841 atggccttta atattacctt gctgctgtat gttgcacctg agacaatatc cacccctaag
     6901 gactgcgctt ctgtaaccct gtccgggatt acttcagcgg ctgccccctg gccgttaaag
     6961 tgcttgacca gctctatcgc gactatctta tgattctcta ttgtcaccct tacttcagct
     7021 tctaccggaa aaaccttata gctgccttta tataccccat ccgcaatctt tgacaaatcc
     7081 acatccggta tgcttgtttc agccagtttt acaaggcttg cttcagtata tgatttgaac
     7141 ccgaatacag cagttacaat tgctgcaagg gctataacca cagccagcac tactcttctc
     7201 ctgcttttca tttatttccc tccttacatt cacttaatga cataaacaat ccggaaaact
     7261 tctcagccag attatcaatc attccttttc gatgtatgtt ggaccagttt acaacaccgg
     7321 ttcctataac atcaaatcct tttgagctgc atatctcttt catccgttcg attgcttggt
     7381 ttcctcccat ttggggaagc ggaaaaaact ccgtcacata acaagctgct tttttatccg
     7441 gaatcgccat aagctgagtc aagtaagctt ccattaccac tgaaggcgag aatccgtgca
     7501 ccggagcccc aaatatcaat gcatcgtaag gatttatatc cggcttgctt ttcaactgta
     7561 cttttatagc atccatttgt ccgttatcac atgcctctac ctgttcaata cttgcaatat
     7621 gccctttcgc cgcaagggtt ttcattagtt tctccgctac agaatacgta ttcccggttt
     7681 ttgaatgaac gataattcca attttcatat ttgacccctt cccaatatat attcaattac
     7741 tttgtccagc tcactataaa catcttctcc cttataatcc acatcttctg aaaagtataa
     7801 tgttatcagc ccgtgtatgg cataaatcat tgttcttgca agtacttcaa tgtcctctgc
     7861 cctaatcttt cctgaaatca cgaattcctt aaaggtttct tcccacattg catcaaaatc
     7921 gggctcctcc tcattttctc cggtttctcc ggcaggttta ctcaaatgat ggaaatagaa
     7981 aaatctgaac acattagggt ttactataaa atattccaca tacattttga agcacttctt
     8041 gattccatcg acatccttcg tcattccagc catttttttg tataatgctt caacaatatc
     8101 attgatcatc agtttcttaa cttcccaaag cagctggttt agatcggaga aataattata
     8161 tatggttgca taggaatagc ctgccatatc tgcaaccttc ctcactgtga cgctttcaac
     8221 accgtcattt aatattattt ctctggctgc ttccaaaaag tatgtcctga tcctctgttt
     8281 cttcagaatc tttttatctt ccgctgtttt ctccttcata atatcaccta tccccgttta
     8341 tataatctgc agtattaaca cgtatacata ttattaacat tgttaatatt taataacaac
     8401 gttaataata tcatggtttt tattatatgt ctatagcaaa ttacaaattt tttcaaaaaa
     8461 atttaagagc cggaattttt cttccggctc ccgtcactta tttaagaata actgactcct
     8521 attcggtgtc ttcagaactc tccggcgaca tcataaggaa accgcagaaa gctccattat
     8581 caacctgaag agaagcatca acctcttttt ttatctgatt gattcgtttc cgataaacct
     8641 tatagaattc aaaggttggt ttgggactgt atttcaaccc ctccatttat ctgcaggtac
     8701 cggatatacc ctatatattt caattgatga cttaaaaact ccttctctaa ctataggatt
     8761 caaattgctt tatcatataa aaaatccatc cgccataaaa ttattgaaag ggggtatttt
     8821 tgtggattta aaacgctggc aaattattgg ggcagttttc gcccttatta tgggagcact
     8881 tctgcatttt acatatgaat ggtccggtga aaacccggtt gtgggaatct tcagcacagt
     8941 taatgaaagc acctgggagc atttaaagct gttgtttgtt ccggttgctc tatttgcagc
     9001 cctggagtat attgggtatg gaaagcagat tcctaacttt gtaccggtaa aggtgctgtc
     9061 catcctgctg ggtatgatca ctatcataat tgcttattat acttacaccc acatagttgg
     9121 gcaaaattat ctctgggtag atattggaat ctttatttta ggagtacttg ctgcatattg
     9181 gttcagctat agtcttctgc agacagaatt tctcgcctca gagtgtgccg tatgcttggg
     9241 ttgggcagga ttgctgctct tagccttttc ctttgcattt ttcacatttt gtccgcctca
     9301 cgtcgaacta ttccttgacc ctgtaaccgg aacttatggc agatagctaa gctaataaca
     9361 taatagccac ccgtgcggat tatccatagt ccgttgtaat tctctttaat cattcaccca
     9421 taatccgtgc tgattacagc acaaataaat ctttccgcgg tacattttgg gaaaacgtac
     9481 ttcgctgcct tgttccgggt acaatttgac aaagagcact ctatcgtaag caacgtacgc
     9541 tacaaaattg atgtaatgcg ttttgctcat ctcatgtgca aatgtaatgt aaaaatcctc
     9601 ctctatctct tccaccgtta atttgtgagt tccctcagcc ggttttggga taagcggcac
     9661 cagttttcgc ccacagcagg aaacatcgga ctcccctgtg gaagttagtg tgtttccgca
     9721 attggggcac gtataaaatt ttattttttt catatttcct ccgtctgcat tattgggttc
     9781 caaatccccc aataatattt tttcgatgtc tgcgtttaat acttcagaga gttcacgaag
     9841 aagggataca tcagggcatc ccaaaccacg ctcccatttt gaaatggtct tgtcactgat
     9901 attcatagca tcggcaagct gcttttgtgt catacccttt tcttttcgca aaccaaatat
     9961 cagctttcca accttgctgc aatccatatt gtcacctcca cgcattaata ataatagcag
    10021 acggactgta attcaataaa cgctccgtag agtttctatg taagattaaa aacctatgga
    10081 ttctccactt ataactgtag atgaactgcc ccctatcaag ctcctctttc acctgttcgg
    10141 cgtctattct gcccttgtcg atatttatat caggctctga gattatatat gcctttccga
    10201 tcccaatacc cggggataca gccagacctt ttttcattct cccatcagcc ttttacccta
    10261 attatctctc taagcaattc caatccccct attcatcacg gccgtcctta agaaaactgc
    10321 cggaatctcc ggctattatt actcccattt tacgactctc tcatatactt ttttaccctt
    10381 tacaaaggtg gcatgtatgt tgaagctatc atcaaacaac acaaagtcag catccttgcc
    10441 tatgtctatg cttcctttcc tgtcattgac tttcaatacc ctcgcagggt tctcagtaac
    10501 ggtttttatt gcatctatca gccctattcc ggtattggcc gtaaaattcc tcaaggcttt
    10561 gtccaaggta aggacgcttc cggcaaggtt acctgcaggc agcctcgcta cctcattctt
    10621 cactactacc cgctgccctc ccagttcata ttctccatcc tccagcaggc atgccttcat
    10681 agcgtcagtc acaagaatta tcccttccac tccctttacc ttcagaagga ttctttgtac
    10741 tgccggattc acatgtacat tgtctgcaat aagctcacag acaacagaac tgtccattgc
    10801 cgctcccacg gcccccggct ccctgtgatt cagcggagtc attgcattaa agatatgggt
    10861 gatatggctt gcacccttct gaatcgctct catggcctgc tcatagctgg cgttcgtatg
    10921 gcctatcgcc actactatgc cctgactgac acagctttgt atgaactcct cggagccctc
    10981 ctcctcagga gcaatggtta caatcctgat aacatcggac aggccctcaa tagttttaaa
    11041 gtcaggcctt cttatatatt tcccgtcttg cgcaccctta tattcttcgc ttatgaaagg
    11101 gccttccaaa tgacagccta gtatggacgc tcccggagca tcgtccataa gcgtccggat
    11161 tctgtttatg cttttctcaa tcatcggaaa gcccattgtc attgtagtag gcaggatagc
    11221 cgtaacccct gtagctgcaa tagccttgct tatgttcctg agactgctct cattgtcatc
    11281 catggtgtca tttccaatac agccatgtat atgcacatcg ataaaacccg gagatagata
    11341 tcttccggcc gcatcaaaga cctcctccgg ctccaggccc tttaatgcat tgccttccat
    11401 cagccgaagt attttctctt cgaatagcac agtatagcca tcaaatatac tattatcttt
    11461 gattatcctc gcattaacta ttgccttcat ttaatcacct tctatttttg aactgtcatt
    11521 tacaagtgac aatatcgcag ccctagcatt accccccgcc ttttcaaagg ccttaagcgc
    11581 ttcctcctct ttccagccgg aaacagcctc aatcctaccc gatattacac caattatcac
    11641 attatcatct attccaaagg taggagggca ttcggaagca tcaattacag caaccttttt
    11701 gtcctcatta ttaaacagtg ttaccatttc aattgttgac agcttatcta ttttttcagt
    11761 attagcattt atacgcaccg cagtacctcc tccggtctta aaacaaggcg gcataagccg
    11821 ccctgttgtc tgttaccttt ttatggattt tttcattcca tcagctatca gctctgctct
    11881 tgttcccact atattggcct ttcctccaag tctttccacc agtttagcag aaaattcact
    11941 gacattcaag ctgcccatag cgctttcgtc catttcctca tctacccttc cgggagtagg
    12001 aatgtcaagc ttctgaataa cccaaacaaa tattatgtag tatgccaaac caaacacaag
    12061 gcctatcgga attatcatca ccggattggt tgccagcccc cagttaagcg cataatctat
    12121 gaaacctgct gagaagccga aaccatgcag tattccaaag gtatatgata ctgcaagggc
    12181 taatcccgta agcactgcat gcagtacata gagcaccggt gccaagaaca tgaacatgaa
    12241 ctctattggt tctgtaatac cggtaaggaa tgctgtaaat gcagcagaga atagtgcacc
    12301 gccgactgcc ttcctgtttt caggctttgc tgtcctgtat atagccagtg ccactgccgg
    12361 cagggcaaac atgaaaatga ggtagaagcc tttcataaag ttgcccgcgc agctgcagtt
    12421 actatgggca caaaccttct gcatccgaaa aatccgagga actcagtcag cttgttatta
    12481 tagtatccgg catatttcaa gccataattt tccataataa ttaaaaagaa aagaacctcg
    12541 ctgccttaag acaacaaggt tcttttcttt taatatctat ttcaagtcaa agggctttaa
    12601 taatttttca acttcaatct ttttgccctg ctcgtccata accggaacct tcgcaaaaac
    12661 ccagccctct accttgttcg gatctgcacc ggcatctata aatggctgcg aatcaaactc
    12721 cagcataacg tccggtgtac cttcactgct gaaatccttc ccccagatga aactttcgcc
    12781 ggtcggggaa gtcaatgccc agccgttatt cacttcatcc gaggttatat tttccggaat
    12841 tttatttaac agtgcttcga aggatgttac cgcaaatttg cctactacat ctgttgcttg
    12901 acacgctgcc agggataaag ctgcagtcaa aactaaaata ataactaagt attttttcat
    12961 aattaataac ctaccttctt tatttcagca tcttttcaat tgtataaagc agctcgtcga
    13021 ccacatccag ttccccggct tgaatacgtt ttatcaggca ggatttcata tgatttttca
    13081 aaatcagttt gcccaccgaa ttcattgcag agttaacaga ggatatctgg ttcagaacat
    13141 cgtcacaata tacatccttc tctatcattc ccttgatgcc ccttacctga ccctcaatgc
    13201 ggttcagcct ggatatgata tccttctttt ctttctctgt tcggacagtt acattgtggt
    13261 cacaattaca atccaagggt atacctcctt tactgcttat tcacagcact tttacagcgc
    13321 cggcatcata gccggcttca tcgactgcat tcttaagcac agaatcggga acttcctttt
    13381 ccaattccac aattgcgtat ttgccttcca aactcacatc gacctttatt acgccgggaa
    13441 cctctccaag tgcgccctca acatgcttaa cgcagtggcc gcagctcatt ccttcaatat
    13501 taatttgttt tttcattttt attcctcctt aaatttctat ttggtattcc tttcgggttc
    13561 ggttgcaaat ttcgggttac gcgttacgtg tggctcaaat ctcaagtccc atgcctactt
    13621 atatggttta aagcctttca gccttaaggc gtttgtcaat accgaaactg agcttaacga
    13681 catggctgcc gctgccagta tcggatttaa aagcgggcct ccaaagacgt gaagcaatcc
    13741 tgctgcaacc ggtataccgg ctacgttgta gccaaatgcc cagaaaagat tctgcttgat
    13801 attccttatt gtactcttgc tgagctgaat agcagtaggc acatccatca aatcgcttct
    13861 cattaaaact atatctgcag attccatagc cacgtcggta ccggagccga tagcaatgcc
    13921 tatatccgcc tgtgccaatg caggtgcatc attgattccg tcaccgacca ttgcaacctt
    13981 cttgccctca gactgcagct ttttaacttc attagccttg tcctggggaa gaacctcagc
    14041 cagtacccag tcgattccaa cctgctttgc tatagcctcc gccgtcttgc ggttgtctcc
    14101 ggtaatcatg actacttcta tacccatttc atgaagctta tttattgctt tggcgctgct
    14161 ctcctttact atgtccgcaa ctgcaataat gccggcaagc tgattgccaa ttgctgcata
    14221 catcggcgtc tttccttctg aagccaatct gtctgattcg gtttcaagcc cggaaagact
    14281 gatattcctt tctgtcataa gcttcctgtt gcccagtaat atattcttcc cttcgatctc
    14341 aacctcaata ccttggcccg gtattgcatt aaacttgtca accttgagat cttccaggtt
    14401 ttctttctct gccccgcgga ctattgcttc cccgagggga tgctctgagc ctttctcagc
    14461 agatgcggca atctgaagaa gcttgtcctt ctttatgatt tctgttgtaa caatatcagt
    14521 tacctccggc cttccttcgg ttatcgtacc ggttttgtca aatactatcg tctttatttt
    14581 atgtgttgtt tcaagcgctt ctccaccttt tataagcact ccgtattcgg cacccttgcc
    14641 tgttccgacc ataattgccg tcggagtagc caggcctaat gcgcaggggc aggcaataac
    14701 caatactgct ataaaaaccg tcagtgcgaa taccggtgtt tccccgccga taaaatacca
    14761 tgccagcgcc gataaggttg ctattacgaa aacaataggt acaaaatatc ccgaaacaat
    14821 atccgccatt tgcgctatag gtgcctttga gccttgagca tcttccacca gcttaattat
    14881 ctgagccaga gctgtatcgc ttcctacttt tgtagccttg aatctgattg aacctgtctt
    14941 gtttatactt gctgcgaata ctttatcccc cggctttttc tcaataggaa tgctctcacc
    15001 ggtcagcatg gattcatcaa ttgccgtaag gccttcaact acttctccgt ccacaggaat
    15061 ttttgaaccg ggctttactg ctataatatc acccacctct acttcctcaa tgggtatctc
    15121 aatttcctca cccgcttgca ttactatggc tgttttagga gccaggccca tcaatttctt
    15181 gatagcctcc gaggttcttc cttttgatac cgcttccagg gatttcccta aaagaatcaa
    15241 ggttataatt acacctgctg tttcaaagta taaatcctca gcatacataa aatgcccgat
    15301 gcttatctga taggttgagt acaagctgta aagcactgca gctgaggtac ccatcgctat
    15361 caatgaatcc atattggggc ttctgtgcaa tattgcctta aaccctactg tataaaaacg
    15421 ataccctgca gtaactaccg gtactgtaag aagaatctgc accaaagcaa acctgagcgg
    15481 gaaatccatt ggatgcaaag ctttcggcag cggcagtcca agcatatggc ccattgctat
    15541 ataaagaagc ggagcagcaa aaactgccga aacaataaac tttgtccata gaattcggat
    15601 ttctctgtcc ttgcgctttt tatcctcgtc taccttatcc ttgctctcta tttcaagagc
    15661 cttatagcct gcctttacaa tagcctgctt tatttccgac agcctcagct cagccggatc
    15721 gtacttgaca tttgcttttt ctgttgcaaa attgactgaa acagcctcaa ctccggaaag
    15781 cttgccgact gccctctcta ttgattttgc acaggcagca caagtcatac cttcaatcgg
    15841 tattgaaacc tccttggtgt ttttttcctg taaagcttca taacctgctt ttgaaacagc
    15901 ttcagcaatg tcgcttggtt ttaactttgc ttcatcatag gtaagggaaa gcttttctgt
    15961 cgctaaatta accgtagcat ctgacactcc gtccagcttc ttaactgctc tttctactgc
    16021 tttggcacat gctgcgcatg tcataccatt tatcctgaaa atatctgatc gcatcatatt
    16081 tctcctttcg tatttacttt acccccctac cctatctgta tataaatata atatcacatt
    16141 atggagattt tatgaagatt catttattta ttttccccaa tctccataaa atcaacatat
    16201 ttaatatata tactatatct cacaagtaca ttaaggagga tagctatgag cattaagaaa
    16261 gaaagaatca aaatttttga aatgacctgc acctcttgtg aaaaccgtgt tgaaaaagcc
    16321 gtcaggcaat tagagggggt tatatatgcc aaagccagct acagcggcca atatatggaa
    16381 attgaatatg atgatgattt atgcggaatg aataatatta aagatgctgt aaaaaatgcc
    16441 ggatacagca cagaaggttc taaggactgc aaattcatgg ggatccttat aattgcggtg
    16501 ggtgtagtgc tgctgggatt aaaaaccggt ggttttgaca tggaggcaaa acttaccaat
    16561 gcttcctatg cagttctttt cattgtaggg gttcttactt cacttcactg tataggtatg
    16621 tgcggcggaa ttatgctttc acaaagcttg tcaaaagaaa gcaaaaacag gttcgaagcc
    16681 atccagccgg ctctgttata taatatcggg agagtcatat cttacactat tcttggcggt
    16741 cttattggcg cccttggttc agtatttacg ctttctattg ctgcaaaggc cgccgtgcag
    16801 atatttgccg gtgcatttat gattattatg ggtttaaata tggcaggctt cagcgctttc
    16861 aggaaatttc agattaagct gcctcaattt gcatgtaaag caaaatcaaa gtccggctct
    16921 ccgctaattg tcggcctttt aaacgggcta atgccttgcg gcccgcttca gaccatgcag
    16981 cttttcgctc tgggcacggg aagtgctgca aaaggggccc tggcaatgtt tgtattttct
    17041 ataggaacag taccccttat gctgaccttc ggtgcactat caggactgct cagcaaaggt
    17101 tataccaaga aaatacttaa attcagcggt gtgcttataa tagttctcgg cctgatcatg
    17161 ggtaaccgag gcctcgcgct ggcaggtata aatattaatc caataactgc ctttgcaagc
    17221 aattcagcag gctcttcaaa tccatatgtt gcgaaggcga ctttacagga tggtatacag
    17281 attataaata tgactgctga taataacggt tatgcgccta atgcttttta tgtccaaaaa
    17341 ggaataccag tcagatggat aattgacgga aaagaattga attcctgtaa taatgcgatt
    17401 gttgcacgag gattgaatct tgaacggaaa attaaaaagg gagaaaacat tatagcattt
    17461 acaccgggag ataaggatat aaacttcagc tgctggatgg gtatgataag cggtgtgata
    17521 aaggtcgttg ataatcttga ttccgtagat acctcgaagg ctgatccgtc cctgcctccc
    17581 ccaagtacag gcccaagctg ctgtgcagta ccgattgaag aggatgaacc aagccggccc
    17641 agtatatacg gcgatgatat aagcaaggtg cccactgaaa agttagttaa taaagcaatg
    17701 gcagacggga agaatcaaag tgtgatattc aaaggtatcg gttatgaatt acagcctttg
    17761 atagctgttg caggcagcgg cgggaaaatc aggctgaccc ttgatttaaa cgattttgac
    17821 aatgccgaag gggagtacct gataatagat gcggcaacag gaaaagatgt tacggctttt
    17881 actgccgaaa aaggtataaa tgaaatcgag ttcagtccaa aaaatgttga tggttatgca
    17941 attataaagg gtaattctat actaggcata attgaggtag ttgacaatct tgaggctgcg
    18001 gatataaaag aaatcag
//
LOCUS       DAOQUR010000043        17973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3580_length_17973_cov_9.454850, whole genome shotgun sequence.
ACCESSION   DAOQUR010000043 DAOQUR010000000
VERSION     DAOQUR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17973
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_17973_cov_9.454850"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..872
                     /locus_tag="PKI50_09085"
     CDS             102..872
                     /locus_tag="PKI50_09085"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT03893.1"
                     /translation="MDKVVELKNVTMNYHTLDGETQAIKNLSLDIRRGEFVSIVGPSG
                     CGKSTLLSLISGLIKSSTGEILINNEPVKGLASNVGYMPQRDHLFEWLTILRNTTIGL
                     EIQKKLNKDTMRNAERLLEEYGLGEFKYYYPNQLSGGMRQRAALIRTLAVNPEILLLD
                     EPFSSLDYQTRLAISEEIWLIIKKEKKTAILVTHDIAEAVSMSDRIIVLTRRPGEVKS
                     EHVIQLTTCENKTPIICREAPEFRIYFNKIWKELDVHV"
     gene            865..1665
                     /locus_tag="PKI50_09090"
     CDS             865..1665
                     /locus_tag="PKI50_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT03894.1"
                     /translation="MYEEYSNEHREYIRKERITKNAVTISRIIILSAIFALWEIAANM
                     KWIDPFIMSQPSRIVNTVINLSKDGSLFLHTGVTIYETIIGFVSGTVLGTLAAIVLWW
                     SNFTARVLDPYLVVLNALPKTALGPIILVWIGGTTGSIIVMALLLSIIVTILNVYQSF
                     RSCDEDKIKLLKTFGATKVQILRKVVFPSSIPEIISTLKINVGLSLVGVIVGEFLVSK
                     AGLGYLIIYGGQVFKMDLVMTSVIILAVAAAFMYLSVSWIEKKFVKMR"
     gene            complement(1959..2597)
                     /gene="ppaX"
                     /locus_tag="PKI50_09095"
     CDS             complement(1959..2597)
                     /gene="ppaX"
                     /locus_tag="PKI50_09095"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase PpaX"
                     /protein_id="HNT03895.1"
                     /translation="MGYKYVLFDLDGTLINTNRLILDSFKYTYKKCLGLEVCEEDILK
                     CFGEPLKVTLRRYSEEKADELFNTYIEYNESRHNDTVTIFDGIQELLSELVNQGRTLA
                     LVTAKRRRVAIMGLELFDIKKYFDVIVALEDTELHKPNPAPVIKALELLGAKPSETLM
                     VGDSVFDIHCAHGASVAAALVKWSAARDFQDDASSADYVVHDTKELLDIIIG"
     gene            2855..4072
                     /locus_tag="PKI50_09100"
     CDS             2855..4072
                     /locus_tag="PKI50_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNT03896.1"
                     /translation="MLNLVLIAMNFALISYYSEPLDYMALYICGGLAAAYMISRMILA
                     KLLNDGNCLMFDVVCNMLQTGLAMLYRLSPEYGTKQAYIAAAGILGFFASFIFMKRFE
                     VKYKHHLLLGGLILALLLTTQIWGIEINGSKNWINLYFTNIQPSEFIKLIFVFFTACL
                     LSREPSRKRFAGIAASMLTVTAFFVLQRDIGSAFLFFTVYLVMLFSRDVRGLYTAASS
                     GAALGGAFISYFAFSYIRSRVLAWINPWKYISGKSYQITQSLFAVATGGFIGTGLYLG
                     DPKFIPAVHTDFIFSALCEETGILNGISLLAMYALLSIIGMDISSKCSNPIYSSAALG
                     LTSLTAIQTLVIVCGVLNIIPITGVTLPFVSYGGSSLLSQFMNVGILYYISTDKASQT
                     SEAESLVPRTASR"
     gene            4084..5466
                     /locus_tag="PKI50_09105"
     CDS             4084..5466
                     /locus_tag="PKI50_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNT03897.1"
                     /translation="MKDRIKIIKYIYLSLFALICLYLLYFSLFESRLIASNSYNRRLR
                     ESEGNIIRGSILDKNGEILAKSEKYKAGVKRTYVYPRYFSHVIGYSSAKYGSSGLEAL
                     YNKELMSASDTMEDLRSKINDYYIKGNNINLTLDRSLQVYASELLRGKKGAVVVLKPD
                     TGEILALVSKPDFDPSGIQNNWESLINDKKSPLLNRASEGLYPPGSIFKLAVASSILK
                     NNLQDIEVECTGEVNADGYIIKDSDGKAHGSITLSEALKHSCNSYFIKQGLKLGNSNL
                     YNEASRFRLNKKIQTDIRVSESVFPSKSDNRSIAQQSIGQGEVLMTPLHAAMMAAAIA
                     NDGVMMQPYVVSSISGSENKIIKEFRPKQIGRIMDKNTAEAIKDMMVEVVESGTGTRA
                     KVSKVAVAGKTGTAEVSGNQKSHAWFVGFAPADSPKAAIAVLIENGGGGGSAAAPIAS
                     KILKKALSLR"
     gene            complement(5586..6920)
                     /gene="mgtE"
                     /locus_tag="PKI50_09110"
     CDS             complement(5586..6920)
                     /gene="mgtE"
                     /locus_tag="PKI50_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNT03898.1"
                     /translation="MENIIKDYIEVKNYSAIKKALEDMLPADIAELLEELDTKNSLLV
                     FRMLHKDVAVEVFAHLSVKQQTSFTLMIKEDELKEILEELYFDDKIDYLEEMPSSVVK
                     KILKYSPETERKLINQFLNYPENSAGSLMTIELVDLKKELTIDEALLRIRRTALDKET
                     IYTCYVTDSNRKLEGTVSLKDLVIAPGNKKVEDIMETDPIFVRTIDDQEEVANLFKKY
                     DLLSVPVVDQENRLIGIITIDDIVDVIEEEASEDIQIMHGLAPSEEEYLKTDIITLAK
                     QRIFWLLFLMLSATITGNIIESFQGSLEKAVGLAVFIPMLMGTGGNAGTQSSTLVIRG
                     IALGEVVLSDISKVIWRELRISLMVGISLAVVNFVRVYYLGGNSFTVSVVVSVTLILT
                     IMIAKVIGGVLPLIAKAINIDPAIMASPLISTIVDALTLLSYFKIAEAIMHI"
     regulatory      complement(7241..7409)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(7560..8264)
                     /gene="sfsA"
                     /locus_tag="PKI50_09115"
     CDS             complement(7560..8264)
                     /gene="sfsA"
                     /locus_tag="PKI50_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNT03899.1"
                     /translation="MKIINKTINGRFVGRLNRFEGIAEINGGEVPVHIPNTGRCRELL
                     FKGAGIILEIRESKTRKTPYELIMVYKGDRLISIDSQAPNKIVEEAVRGGLIEEIGSY
                     DYVKREAFYGQSRFDLLLKKTEGSGKADSCYMEVKGVTLEINGTALFPDAPTERGARH
                     MKELAAARKEGYRAVVIFLVQMEGIKCFTPNKLMDSGFAEALADAHSEGVEVLSYNCR
                     VSEKEIVINERVEVVL"
     gene            8381..8812
                     /locus_tag="PKI50_09120"
     CDS             8381..8812
                     /locus_tag="PKI50_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03900.1"
                     /translation="MDKYNILVLMEKNRATGFFIQTIDSYKIDSGLELIENAYLSEEE
                     GEYFIFLVLTTADVEDYQYNGIYDLYNEEVFSKFNIDLLDGSGEYNPRWIVKLKYNEK
                     RSEMESIINELLEIHKAELQRVLPLVEADKTKYLEEFEKEE"
     gene            complement(8844..10796)
                     /locus_tag="PKI50_09125"
     CDS             complement(8844..10796)
                     /locus_tag="PKI50_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HNT03901.1"
                     /translation="MDKDKILKEQVQELDLDEIIAKYDKESNYRKLSGIQLKIVTALA
                     IMFSVFQLYTAIFGVLPAQLQRSIHITFALVLAYLLYPSSEKMSKHKMHWLDVVLACI
                     AGFVGLYIPFNYHALILRAGEQNTTDIIIAVIAVLFVLEAGRRVVGLPIVIIASTFLV
                     YCVVGPYLPGFLNHRGYSVARIVSHMYYTTEGILGTPIGVSSTFIFLFILFGSFLDKT
                     GIGKFFIDLANAVAGKAIGGPAKVAVLSSALTGTISGSSVANTVGTGSFTIPLMKSLG
                     YKPEFAGAVEAAASTGGQLMPPIMGAAAFLMSEFIGIPYGSIAKAAIIPALLYFSGIW
                     IMVDLEARKTGMKGLEESKLPKLSKVLGERWYLFTPIFVIIYMLMSGTTPIKAALYGI
                     YSSILVGMIRKETRMNLKSLLQALETGAKSALGVAIACACAGIIVGTVTLTGLGLKMG
                     NGLVELAGGLLLPTLFLTMISSLILGMGAPTTANYIITSTIAAPALLKLGVNPLTAHM
                     FVFYFGIIADITPPVALAAFAGSAIAKSDPIKTGINASKLAIAAFLIPYIFVINPQLL
                     LINTTGLEVIQITITSLIGMFGIGIAMEGFIFGKVNPLIRLIFMAGGLCLIDPGVYTD
                     MAGIIAIGLGLAYQWFKYKRTTAKSI"
     gene            complement(10859..11323)
                     /locus_tag="PKI50_09130"
     CDS             complement(10859..11323)
                     /locus_tag="PKI50_09130"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HNT03902.1"
                     /translation="MLIIILLNVPVLNRFTISNGRTGKIVFIDNTENIREFTVSFKHS
                     VNRTLVNEFIRIQGDRFVVYKTTFYSYGAGMPEYDANSKQVITISDGIVQIENIDRKL
                     DSFTYMVGTYADHTLSYKDKTMKLSEFIEPQKPAYFKIQKVSCIELINYLLR"
     gene            complement(11405..12361)
                     /locus_tag="PKI50_09135"
     CDS             complement(11405..12361)
                     /locus_tag="PKI50_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNT03903.1"
                     /translation="MKKYIVLTLAVVLVMSAALTGCAPQKTNLILATGGTSGTYYPFG
                     GSMAQIFNSKIPNMNVTAQATGASVENLKLISQKEAELAIVQNDMTDYAYNGTESFKD
                     SKVENVRVIANLYPEVIQIVSSADSGINTLTDIKGKKFSVGAPGSGVEANARQLLEVM
                     GMTYNDFSANYLSFAESADSLKDNHIDGFLFVSGIPNAAIQDTATTSSLNFVSIPDDV
                     INKLIEKYPFFTEYIIPAGTYKGQTGDVKTVAMKATLVAGAEVSEKVIYDLTKALFEN
                     QPELASAHAKGAELSLESAVDGVSVPFHPGAEKYFKEVGAIK"
     gene            complement(12485..13378)
                     /locus_tag="PKI50_09140"
     CDS             complement(12485..13378)
                     /locus_tag="PKI50_09140"
                     /inference="COORDINATES: protein motif:HMM:NF020248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT03904.1"
                     /translation="MTSFIVIDDDRTVRSVLIKIIEQYSLGEVYAEADNGLLGEEMIL
                     RYKPDIVIIDFLLPHQDGMSIIKKVKSLNVKSMFIMLSQVSDKDMIARAYEMGIEFFI
                     SKPINVIEVVNVIRRVREYLAMKKTFEAIESTVMNLGRSADSEPSRKSGVVKRLLNQI
                     MADLGILGDLGSRDIMNICTMLYENPEYGDTLEETQLSDLLKLLQSKYTSEGRVSKSD
                     FKAIEMRMRRTVAKAMKNIAAMGIEDFSNDKFVLYSSSLFDYVEVKAEMDYIRGKAMY
                     NGKVNIKSFIKRLLVMLEQIA"
     gene            complement(13371..14666)
                     /locus_tag="PKI50_09145"
     CDS             complement(13371..14666)
                     /locus_tag="PKI50_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT03905.1"
                     /translation="MRNKYKEMLIVGMCTALMGQVNFYPFGTDFRISIGVVAFTFLML
                     YFHTIPIISSSIITGLSVLMLRVGIDVFANSMAMDATFYKHMPGMMYYITYGIIIEGS
                     SFRKLFEKPIYFIMVLITADILSNIFELLIRNQLNANSFDTIFSTIMLAAVIRSCLVL
                     ALFWIIKYYSLFVTKEEHQRRYKELLLLTSKLKSEIFFLKKSMQDIENAMAKSYSIYN
                     TIKESNPMGKEDIEIITADCLNLSIDIHEIKKDYNRIVISMEKLMPAEDTHKTMSISE
                     VFETIGDIFNRYIEVINKDITLSFEIEKDYKIKEYFIIMSILNNLIQNSVEACFRSDS
                     SIKVGCFIHESMLVFAVSDNGKGIGKKERELIFEPGFTTKFNPETGHVSTGLGLTHIK
                     ILLEHLEGKININEDIEGITKFVIEIPINKIVGEVEDID"
     gene            14896..15855
                     /locus_tag="PKI50_09150"
     CDS             14896..15855
                     /locus_tag="PKI50_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNT03906.1"
                     /translation="MKNKVINLALTIISVSSLLFILTTSVFADFGDSTLNKGITHPDV
                     HVLQEKLNTLGYFNNGQFTDYFGDKTYNALIAFQRDEGLNPDGIAGNETFKRLSLKIT
                     QAEILPKDFVPIKEGDIGDKVVDIQKKLRDLNIYKTEINSFYDQTTIEAVISFQAANA
                     LPRTGIVDSATIIKLNTAYNSILADRASASRALLNSKVVEYAKQFLGKPYVWGASSGK
                     AFDCSGFTVYIMKKFNVSLPRTASGQINSGTKVGKDDLQAGDLVFFTTYKKGPSHVGI
                     YIGGNKFIHASSGVDKVTITDLDSSYYKSRYLGARRYSLTDTK"
     gene            16011..17105
                     /locus_tag="PKI50_09155"
     CDS             16011..17105
                     /locus_tag="PKI50_09155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNT03907.1"
                     /translation="MSINSTSKSKQKELIKKLRSSNILLKRYSMITDSIMKITSEVFS
                     SGEIDYILQTIMDKAIEIIPGAQRGSILIYNGSVLEFKASRGYDSSALENLKLSIEEV
                     FQYNSEDFFEPCIVSNQERFNREHLPDYKYEILQKGLGINPKSTLSCAFQVDNKFQGI
                     INLDNMDDNTAFRKEDKPLIKHLAVQIGIALKNARQIEKILFLSRHDSLTGIYNRCYF
                     EELLMHIMQTSKASASVFSLAMLDINNLKYINDTYGHEAGDLMLKKFAQCVNEIITGD
                     DIFARYGGDEFAIIFKDNDNIQAANKINNIRQTLLNLPFTYCGKTIAPIGFGCGIVQF
                     PADGSEFQDFMRLADISMYQDKKKSKKPVI"
     gene            complement(17129..17842)
                     /locus_tag="PKI50_09160"
     CDS             complement(17129..17842)
                     /locus_tag="PKI50_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03908.1"
                     /translation="MNIIEYLKQQKVIPQYKADKFIELLATYEENDVLYENAIKQLDL
                     TNDEYDRLFTELEKGNYVDRIYIIVCPYCDSLGNTYVQSFDIPETDKCRFCHNVFNHK
                     ENNMEAFRLYFNYHKNIDILTELSQKINYNQERIFEIAEEIKELETKRKSLQDENIKF
                     VTLRNELQYDKSESKAYEKTVDKYKEDKVKYIGTLIYIKKCVAKCSKCNEAFELFNVN
                     GKYIGVCPSCDFEIELLMK"
BASE COUNT         5278 a         3870 c         3373 g         5452 t
ORIGIN      
        1 taaaaagcaa ctgtaccata cctgtcaatg attcttttta ttcactaata ccactgctga
       61 tgaatagtat ggagttataa gaacttactg ggggtgtagc agtggataag gtagtagagc
      121 taaaaaacgt gaccatgaac taccatacac ttgacggtga aacccaggcc attaaaaatc
      181 tatctctgga cataagaaga ggagaattcg tcagcattgt cggccccagc ggctgcggca
      241 aatctacttt gctttctctt atttcggggc tcataaaatc ttccactggt gagattttaa
      301 ttaataatga gcctgtaaaa gggttggcaa gcaatgtcgg atatatgccg cagagggatc
      361 atctgtttga atggctcaca atactcagga atactaccat aggacttgaa attcaaaaga
      421 aactaaacaa ggatacaatg agaaatgctg aaagattgct ggaagagtat ggcctcggtg
      481 aattcaagta ttattatccc aatcagctct caggaggaat gcgtcaaagg gcggctctga
      541 tacgaacctt ggctgtcaat ccggaaatac tgcttctgga cgaacccttt tcgtccctcg
      601 actatcagac aagacttgct atttctgaag aaatctggct tattataaaa aaggagaaaa
      661 agacagcgat tcttgttact catgacatag cagaagcagt atcaatgtca gacaggataa
      721 ttgtgcttac aaggaggccg ggggaagtta aatcggagca tgttattcag ctgactacct
      781 gcgaaaacaa gactcctatt atctgcagag aagctcctga attcaggata tatttcaata
      841 agatatggaa ggagctggat gttcatgtat gaagaatatt ccaatgaaca tagggaatat
      901 atcagaaaag aaagaattac aaaaaatgcc gtgactattt ccaggataat aatactttca
      961 gcgatatttg cattatggga aattgccgcg aatatgaagt ggatagaccc cttcatcatg
     1021 agccagccct ccaggattgt aaatactgtc ataaatctct ctaaggacgg ctccctgttt
     1081 ctgcataccg gagtcaccat atatgaaacc ataattggtt ttgtatccgg cacagttttg
     1141 gggacacttg ctgcaattgt cctttggtgg tctaacttta cggcaagagt ccttgaccca
     1201 tacctggtag tgcttaatgc actgccaaaa acagctttgg gcccgattat actcgtatgg
     1261 atcggcggaa ccaccggctc aatcatagta atggcccttc tcttgtctat tattgtcacc
     1321 attctcaatg tctatcagtc cttcagaagc tgtgacgagg ataaaatcaa gctcttaaaa
     1381 acctttggcg ccacaaaggt tcaaatactg agaaaagtgg tattcccatc cagtattccg
     1441 gagataattt caacgctgaa gataaatgta gggctgtcac tggtaggcgt aattgtcgga
     1501 gaattccttg tatcaaaggc aggcttgggc tatctgataa tctatggcgg ccaggtattc
     1561 aagatggacc tggtcatgac cagcgtcatt atactggctg tggctgcggc gtttatgtat
     1621 ctgtcagtgt cgtggattga gaagaagttt gtaaaaatgc gctaattaga ctgtagcaga
     1681 ataatagaat agtatgtaaa gtactccggc tgtaagggaa gtatgttctc ctcaccttcc
     1741 aaagcgggag atgcaaggag gagaagcccc ctgccgataa aggagggtgc tcaggatgct
     1801 tgcatccgtc gaagggaacc atagggttcc ctagggtcgg aaaagcgtcg tgagactagc
     1861 ttttcctctg gggtattctc gttccggggt caggtggaag gtttcgtcga agatatcttc
     1921 cccacgtcgg tactctgcct tccactaact tcaaagtgct accctataat aatatctaac
     1981 agctcctttg tgtcatgaac tacataatcc gctgatgaag catcatcctg gaagtcccgg
     2041 gcagcgctcc atttcaccag agcggccgca acagaagctc catgggcaca gtgaatatca
     2101 aaaacactgt ctccgaccat taatgtttcc gagggcttcg ctcccaaaag ctccagggcc
     2161 tttatcaccg gagccgggtt tggcttatgc agttccgtat cttcaagtgc cacgattaca
     2221 tcaaagtatt tctttatatc aaataactca aggcccataa tcgctactct tctccttttg
     2281 gcggtaacaa gggcaagggt acggccttga ttcaccaatt cggataaaag ctcctgaatc
     2341 ccgtcgaata tagtcactgt atcgttatgc ctcgactcat tatactcaat atatgtatta
     2401 aacaattcat ccgccttttc ctcggagtat ctcctcaatg tcactttcag aggctccccg
     2461 aagcatttca gtatatcctc ctcgcagact tccaagccga ggcatttttt ataagtatac
     2521 ttaaaggaat ctaaaatcag cctgtttgta ttaatcagag taccgtcaag atcgaataat
     2581 acatatttat aacccatatt atgctccttt acatcttaat tcaatgagat tattctagca
     2641 tatattggag tatatgtgaa tgcgcaggtg aattttgccc aatattattg tataatatat
     2701 tattgttgga attttaccag aaaccggtga tattgtgttt gaagtttttt catggagcta
     2761 cagattcata tatacgctta taactgccgg ctttgctttg tcgtgctgga tgacatacta
     2821 taagaatatt cgtatatgca ggtatgtttc cccattgctg aatttggttc ttattgcaat
     2881 gaacttcgct ctgatttcat attattccga acccctggac tatatggctt tgtatatctg
     2941 cggcggcctt gccgctgctt atatgatatc aaggatgatt cttgcaaaac ttttaaatga
     3001 cggcaattgc cttatgtttg acgttgtctg taatatgctt cagacagggc tcgccatgct
     3061 gtacaggtta agcccggaat acggtacaaa acaggcatat attgcagccg caggcatact
     3121 tggatttttt gcttccttta tttttatgaa aagatttgaa gtaaagtata agcatcattt
     3181 gctccttgga ggtttgattc ttgcccttct gttaacaaca cagatatggg gtatagaaat
     3241 caacggctca aaaaattgga taaatcttta tttcacgaat atacaaccct ccgaattcat
     3301 caagctgatt tttgtgttct tcacagcctg tctgctgtcc cgggaaccaa gcagaaaaag
     3361 atttgccgga attgccgcat ctatgcttac cgttacagcg ttttttgtcc ttcaaaggga
     3421 tataggctcc gccttcttat ttttcacggt ataccttgtc atgcttttct ccagggatgt
     3481 taggggctta tatactgcag cctcatccgg tgcagcatta ggaggggctt ttattagcta
     3541 cttcgctttt tcatatataa gatcccgggt tttggcatgg ataaatccct ggaaatatat
     3601 atccggcaaa tcctatcaga taacacagtc actgtttgca gttgctacag gcggcttcat
     3661 cggtactggt ctgtatctcg gagacccaaa attcatacct gctgtccaca ccgatttcat
     3721 attttccgca ttatgtgagg aaacaggtat attgaatggg atatccctgt tagccatgta
     3781 tgcgctgctc agcataatcg gtatggatat aagctcaaaa tgcagcaacc ccatatattc
     3841 atctgcagct ttgggcctta cctcccttac cgcaattcaa acccttgtaa tagtatgcgg
     3901 tgtactgaac ataataccca taacaggtgt cacacttccc tttgtaagct acggcggaag
     3961 ttcactgctt tcacagttca tgaatgtggg aatattgtat tatataagca cagacaaagc
     4021 ttcacagaca tctgaagccg agagcctcgt gccgcgcacc gcgagccgat aggagggatt
     4081 tgtatgaaag acagaattaa aattattaaa tatatatact tgtccttatt tgcgcttata
     4141 tgcttgtatc ttctgtattt cagcctgttt gagtccagat tgatagcttc caactcatat
     4201 aacagaaggt tgagggaatc ggagggaaat ataataagag gaagcatact ggataaaaac
     4261 ggggagattc tggcaaagag tgaaaagtat aaagccggag ttaaacgcac atatgtctat
     4321 cccagatact tttcccatgt tattggatat tcaagtgcca aatatggttc atcgggcctc
     4381 gaagcccttt ataacaagga gcttatgagc gccagcgaca ctatggagga cttgagaagc
     4441 aaaatcaatg attattatat aaaaggcaac aatatcaatc tgacactgga tcgttccctt
     4501 caggtttatg cctccgaatt gctgcggggc aaaaaaggag cggtggtagt actgaagccg
     4561 gataccggag aaatacttgc cttggtaagc aaaccggatt ttgacccctc cggcatacaa
     4621 aacaactggg aaagccttat aaatgataag aaaagccctc tgctgaacag agcttcagag
     4681 gggctttacc ctccgggttc gatattcaaa ctggcagtgg cttcctcaat actgaaaaac
     4741 aatttgcagg acattgaggt ggaatgcacg ggagaagtaa atgcggacgg ttatattatc
     4801 aaagacagcg acggcaaggc tcacggcagc ataacccttt cggaggcctt gaaacactcc
     4861 tgcaattcat atttcataaa gcaagggctt aagctgggaa acagcaatct gtacaatgaa
     4921 gcgtcaaggt tcaggttaaa caagaaaata cagacagata taagagtatc cgaaagcgtt
     4981 ttccccagta aatctgacaa caggagcatt gcccagcagt ctataggaca gggtgaggtg
     5041 cttatgacgc cccttcatgc tgcaatgatg gcagcggcca ttgcaaatga tggggttatg
     5101 atgcagccat atgtagtaag cagtatcagc ggcagtgaaa ataagataat caaagaattc
     5161 agaccgaagc agataggcag gataatggat aaaaacaccg ctgaagccat aaaagatatg
     5221 atggtcgaag tggttgagtc cggcacggga accagggcta aggtcagcaa agttgccgtt
     5281 gcgggaaaaa caggcactgc cgaggtcagc ggcaatcaaa aatcccacgc atggtttgta
     5341 ggctttgccc cggcagacag tccgaaggct gcaattgcgg tacttattga aaatggcggc
     5401 ggaggcggca gtgcagctgc acccatagcc tcaaaaatac tgaaaaaggc acttagccta
     5461 agataaataa cggataacac tgaaatcaca gaaatccact gaatgcacgg cggaaattat
     5521 attaatttgc cgtgaattca gtgcaaaatc ggtgttctct gtgttacccg ttctcagtgt
     5581 ctgtttcata tatgcataat cgcttccgct attttaaaat aggaaagcag tgtaagtgca
     5641 tccacaatag ttgaaatcaa tgggctggcc attattgccg ggtcaatatt aattgctttg
     5701 gcaatcagcg gcagtacgcc tcctattacc tttgctatca taattgtaag tatgagtgtg
     5761 acgcttacca ccacagatac tgtaaaagag ttccctccaa gataatacac ccgtacaaaa
     5821 ttcactactg ccagcgaaat tccaaccatc aggcttattc tgagttctct ccatattacc
     5881 ttcgatatat cgcttaagac aacttctcca agagcgatac ctcttattac cagtgttgat
     5941 gattgtgtcc ccgcatttcc tccggtaccc ataagcatag gtatgaatac tgcaagccct
     6001 actgccttct ccaggctgcc ctgaaagctt tctattatgt ttccggtaat agttgccgaa
     6061 agcatcagga acagcagcca gaaaattctc tgctttgcca gggttattat atcagtcttc
     6121 agatattctt cttcactggg tgccaaaccg tgcatgatct gtatatcctc tgatgcctct
     6181 tcttcaatta cgtccacgat atcgtcgatg gtaatgattc ctatcagcct gttttcctga
     6241 tccacaaccg gtacggacaa aaggtcatat ttcttaaaga gattcgccac ctcttcctgg
     6301 tcgtcgatag tccttacgaa tatgggatcc gtctccatga tatcttcaac ttttttattc
     6361 ccgggtgcaa ttaccaggtc cttaagggag actgtaccct ccagctttct gttactgtcc
     6421 gttacgtagc aggtatatat ggtttcctta tccaaagctg tccgcctgat acgcagcagc
     6481 gcttcatcaa tggtaagctc cttcttcagg tcaaccagct ctatcgtcat caggcttccc
     6541 gctgagttct ccggataatt caagaactgg ttgataagtt ttctctctgt ttccggagaa
     6601 tacttcagta tcttttttac aacacttgag ggcatttcct caaggtagtc aatcttatcg
     6661 tcgaaataca gctcctccaa tatttctttt aactcgtcct cctttatcat aagggtaaaa
     6721 ctggtctgct gttttacaga aaggtgtgca aatacttcta ctgcgacatc cttatggagc
     6781 atcctgaaaa ccagaaggct gttctttgta tcaagttctt caagaagctc tgcaatatcg
     6841 gccggcagca tatcctccag cgcttttttt atggctgaat aattctttac ttcaatataa
     6901 tccttaatga tgttttccat agtctttcct cccaacaatg ggaaaagcgg ctgtcattgg
     6961 tgaaataaac agactttccc ctatagtctt tccatattct actgcaataa ttactgggcc
     7021 cagcgtccat ttaaatcacc tcaccgtagg ctaattttaa ttagatattc tttttgccgg
     7081 ctgctcgtgg gtatccacgt gcagtgaagc aaaatataga atatcttatt aaaatttgcc
     7141 gttgtattat ttactgctta gcatataaat cttaacaagc ttctaacaaa aacaaaatcc
     7201 tttcactgga gtgaaaggaa tagtatactg taacagcatt acatttcttc actcgcggag
     7261 ctttggcact atacagcttt ggatagaagt tttccagctg cattaagtag aaccttaatt
     7321 cgataatact tgttaacccg ctggcatctc tcgatgtttc cgggtagcag catttatccg
     7381 tataggagcc tcacctaacg aagattcaat tgtcattttt attatagccc tatgaaatct
     7441 tcatttcaat atatataata tgaaatttat taaaaaaagt tcatattata ttaaatttcg
     7501 ataatgaaca aaaacatctc ttaaaacttg tatatatttc ggaatttttc atcattgctt
     7561 cacaatacta cttcaactct ttcattaatc actatttcct tctccgatac cctgcaattg
     7621 tatgaaagca cttccacccc ttcggaatgt gcatctgcca gggcttcggc aaatccggag
     7681 tccatcagct tgttgggagt aaagcatttg ataccctcca tctgaacgag gaaaatgacg
     7741 acggctctgt aaccctcctt acgtgcagca gccaattcct tcatatgcct tgcacctctt
     7801 tcagtgggag catcgggaaa caatgcggtg ccgttaatct caagagttac tccctttact
     7861 tccatataac aggagtcagc ttttccgctt ccttcggttt tctttaaaag cagatcaaat
     7921 ctgctttgcc cataaaaggc ttccctcttg acataatcgt agctccctat ctcctctatc
     7981 agtcctcctc ttacagcttc ctcaacaatc ttgttcggcg cctgggaatc aattgatata
     8041 agcctgtctc ccttatatac cattatcagc tcataggggg tcttccttgt cttgctctcc
     8101 cttatttcca gtatgattcc ggctccttta aacaggagtt ccctgcatcg gccggtatta
     8161 ggtatatgga ccggtacttc ccctccattt atctccgcta ttccctcaaa cctgttcaat
     8221 ctgcccacaa atcttccatt aatggtttta ttgataattt tcatttttat actccttgta
     8281 agttgtgcct gaaacggtta aaatataatc acatttaatt attgaattat accatattta
     8341 ttctgcaaga tgccaggcag tatgccccgg aggtaataaa atggacaaat acaacatact
     8401 tgtgcttatg gaaaagaata gggcaacagg tttttttatc caaacgatag attcttataa
     8461 aattgattcg ggccttgagc tgatagagaa tgcatacctc tctgaggaag agggagaata
     8521 cttcatattt cttgtcctca ctacagccga tgttgaggat tatcaataca atggaatata
     8581 tgacttatac aatgaagagg tattcagcaa attcaatatt gatcttctgg acgggtcagg
     8641 agaatacaac cccagatgga ttgtcaagct aaaatacaat gaaaagcgca gtgaaatgga
     8701 gagcattatt aatgaacttc ttgagatcca taaggctgaa cttcagagag tacttccgct
     8761 ggttgaagca gataaaacaa agtatttgga agaatttgaa aaggaagagt gatatcttaa
     8821 tcaactcttc cttattattc cggctaaata ctttttgctg tagttctttt atacttaaac
     8881 cactgatatg ccaatcccag tccgattgct ataatgcctg ccatgtcggt atatacaccc
     8941 gggtcaataa ggcaaagccc gccggccatg aaaattaatc tgatcaaagg atttaccttt
     9001 ccgaatatga agccttccat ggctattcct attccaaaca tacctattaa ggatgtgatt
     9061 gtaatctgaa tcacttccag tcctgttgta tttatcaaca gtaattgagg attaataaca
     9121 aatatatatg gaatcagaaa tgctgcaatg gcaagcttac ttgcgtttat accggtcttt
     9181 atcggatcgc tcttggctat tgcagagcct gcaaaggcag caagggctac cggtggtgtt
     9241 atatcagcaa ttattccgaa ataaaataca aacatgtggg ctgtcaacgg atttaccccc
     9301 agcttcaaaa gtgccggtgc ggcaattgtt gaagtaatta tatagtttgc ggttgttggg
     9361 gcacccatcc caagaatcag ggatgaaatc atagtaagga acaaggtagg cagcaggagt
     9421 ccccccgcca attcaacaag gccgttgccc attttaagtc ctaatcctgt cagagttaca
     9481 gtacctacaa ttattcctgc acaagcacag gctattgcta cccccagcgc gcttttggct
     9541 ccggtctcca atgcctgaag caatgacttc aaattcatcc tggtctcttt tcttatcata
     9601 cctaccaaaa ttgatgaata tattccgtaa agagccgctt ttatcggtgt ggtaccgctc
     9661 ataagcatat atataattac aaatatcgga gtaaacaagt accatctctc gccaagcacc
     9721 ttactaagct ttggaagctt tgattcttca agtcccttca tacctgtctt tcttgcttcc
     9781 aaatcaacca ttatccatat accgctgaag taaagcaaag ccggtattat tgccgctttt
     9841 gctatacttc cgtatggaat tccaataaat tcggacatta agaatgctgc cgcacccata
     9901 atcggaggca ttaactggcc ccctgtcgaa gcagctgcct cgactgcacc tgcgaattcc
     9961 ggcttgtatc ccaggctctt cattaaagga attgtaaagc tgcctgtgcc gacagtgttt
    10021 gctacggagc ttccggatat ggttcccgta agtgcactgc tgagtactgc aacctttgca
    10081 ggaccgccta tagctttccc tgcaacggca ttggccaaat caatgaaaaa ctttcctatt
    10141 cctgtcttat ccaggaagga tccgaaaagt ataaacagaa atataaaggt ggaggatact
    10201 ccaatcggtg tccccagtat tccttccgtt gtataataca tatggcttac tatccttgca
    10261 acggaataac ctctatgatt tagaaatccc ggaaggtagg ggccgactac gcaataaacc
    10321 agaaatgtac ttgcaataat tacaatcggc agcccgacaa cccttcttcc tgcttccaac
    10381 acaaacagca cagctattac agctattatt atatctgtag tattctgttc tcctgctctt
    10441 aatataaggg catgataatt aaagggtata tataatccta caaatcctgc aatacatgcc
    10501 agcactacat ccagccaatg cattttatgc ttagacatct tttcgctgga tggatataac
    10561 aaataagcca ataccaaggc aaaggtaata tgaatcgatc tttgcagctg tgccggcaga
    10621 actccaaaaa tagcggtgta caactgaaaa acggaaaaca tgattgcaag tgccgtaact
    10681 atctttaatt gtattccgga aagtttcctg taatttgatt ccttgtcata tttggcgatt
    10741 atttcatcca gatccaactc ctgtacctgc tccttcagaa ttttatcttt atccatgaaa
    10801 tccctccttt ttcctgtaaa taaattctat atataaatat tcaagttctg atttgtattc
    10861 atcttaacaa ataatttata agttcaatgc atgagacttt ttgaattttg aaatacgcgg
    10921 gtttctgcgg ttcaataaac tctgaaagtt tcattgtttt atccttatag cttagagtat
    10981 gatctgcata ggttcccacc atgtacgtaa agctgtcaag ctttctgtca atattttcaa
    11041 tctgaacaat tccatcagat attgttatta cctgcttgct gttggcatca tattccggca
    11101 ttcctgcgcc gtatgagtaa aaagtcgtct tatatacaac aaacctgtct ccctgtatcc
    11161 ttataaactc atttacaaga gtacgattaa ccgagtgctt gaagcttacc gtaaactctc
    11221 tgatattttc ggtattatca ataaatacaa ttttaccggt cctgccgttg gaaattgtaa
    11281 acctgtttaa taccggtaca tttaataaaa taattatcag caaaagtaaa aaaactgcat
    11341 atataaactt tttcataaat gaagaagccg ggccggatat ttccagcccg gcagccctcc
    11401 caaattattt aatagctcca acctctttaa agtatttctc tgctcccgga tggaacggaa
    11461 ccgatacgcc gtcaacagcg ctttcaaggc ttagttctgc tccctttgca tgggcagagg
    11521 ctaattccgg ctgattctca aataatgcct ttgtcaggtc atatattacc ttttcggaaa
    11581 cttctgcacc ggcaaccaaa gttgctttca ttgctactgt ctttacatcc ccagtctggc
    11641 ccttgtaggt tccggctgga ataatatatt cagtaaagaa aggatacttt tcaatcagtt
    11701 tattgattac atcgtccgga attgaaacaa agttcagact gcttgtagta gctgtatcct
    11761 gtattgctgc attaggtatg ccggatacaa acaagaaacc gtcaatatga ttgtctttca
    11821 gagagtctgc tgattcagcg aaagacaggt aattggctga aaaatcattg tatgtcatac
    11881 ccataacttc caacaactgt cttgcatttg cttcaactcc gcttcccgga gcacccactg
    11941 agaacttctt ccctttaatg tctgtaagag tattgattcc actgtctgcg gaagaaacaa
    12001 tctgaattac ctccggataa aggttagcaa taacccttac gttttcaacc ttgctatcct
    12061 tgaaagactc tgttccgtta taagcgtaat ctgtcatgtc gttctggact atcgccagct
    12121 ctgcttcctt ctggctgata agcttgaggt tctcaaccga tgcacctgtt gcctgagctg
    12181 ttacgttcat gttcggaatc tttgagttga atatctgtgc cattgaaccg ccaaacggat
    12241 aataagtacc ggatgtaccg ccggtagcca atatcaaatt ggtcttctgc ggggcacagc
    12301 ctgtaagtgc tgctgacata acgagcacca cagccaatgt cagtactatg tactttttca
    12361 tagaatctcc tcctccaatt tattattatt agtttattac aacagtaaca aatagtaaca
    12421 aaaaaaaacg tgaatttaca tccacgttga gaaaatttag aaaattgatt ttgaataatt
    12481 atgtttatgc aatttgttcc agcatcacca gcagtctttt tataaaggac tttatattga
    12541 ctttgccgtt atacattgct ttgcccctga tataatccat ttccgccttt acctcaacat
    12601 aatcgaacaa ggacgaactg tagagaacaa acttgtcgtt actgaagtcc tcaatcccca
    12661 tagctgcaat attcttcatc gctttggcca ccgtcctcct cattctcatt tcaattgcct
    12721 taaagtcact cttagaaacc ctgccttccg aagtatactt gctctgcagc agttttaaaa
    12781 gatcgctcag ctgagtttcc tccaaggtat ccccatattc agggttttcg tacaacatgg
    12841 tgcatatgtt cattatatcc ctgctgccaa ggtcgcctaa tatccccaga tcagccatta
    12901 tctgattcag cagcctttta acaactcccg attttctgga aggctcggaa tccgcgctcc
    12961 tgcccaggtt cataacggtt gactcaatgg cttcaaaggt tttcttcatt gcaaggtatt
    13021 ccctgactct tcttataaca ttgacaactt caataacatt aataggcttg ctgataaaaa
    13081 attcaatccc catctcatag gctcttgcta tcatatcctt gtcactgacc tgtgacagca
    13141 ttataaacat actttttaca ttcagggatt tgaccttttt tattatagac attccatcct
    13201 gatggggcag cagaaagtct attattacaa tatcaggctt ataccggagt atcatctctt
    13261 ctcccagcaa tccgttatct gcttctgcat atacttctcc aaggctgtac tgttcgatta
    13321 tctttatcag cacgcttctg accgttctgt catcgtcaat aactataaag ctagtcaatg
    13381 tcttccacct caccaactat tttgtttatc ggtatctcga taacaaattt tgtaataccc
    13441 tcaatatcct cattaatatt tattttcccc tccaggtgtt ccagcaatat ctttatatgt
    13501 gtcaggccaa gtcctgtcga tacatgcccc gtctccggat tgaactttgt agtaaagccc
    13561 ggttcaaaaa taagctcacg ctcctttttg cctatcccct ttccattgtc actgactgca
    13621 aaaaccaaca tgctttcatg tatgaagcac ccgactttta tagaggaatc gcttctgaaa
    13681 caagcttcaa cagagttctg tatcaaattg ttcagtattg acattattat aaaatattcc
    13741 ttgattttat aatctttctc tatctcaaag ctgagagtga tatccttgtt gattacttca
    13801 atatatctgt taaatatatc acctatcgtc tcaaatacct cacttatact cattgtcttg
    13861 tgagtatctt cagccggcat aagcttctcc atgcttataa ctattctgtt atagtccttt
    13921 tttatttcat gtatgtcaat agacaaatta agacaatcag cagttattat ttctatatct
    13981 tccttaccca taggattgct ttccttaata gtattgtata tagaataact ctttgccata
    14041 gcattctcaa tatcttgcat agacttctta agaaagaaga tttcagactt tagttttgaa
    14101 gtcaatagaa gaagttcttt gtaccttctc tgatgctctt ctttagtcac aaacaggctg
    14161 tagtatttta ttatccagaa caaagccaat accaggcagc ttctgattac agcagccagc
    14221 atgatagtag agaaaattgt gtcaaatgaa ttagcattaa gctgattccg tatcagcagc
    14281 tcaaaaatgt ttgacagtat atctgccgtt atcaatacca taataaaata aatcggcttt
    14341 tcgaagagct tcctgaaact cgatccttca ataattatgc catatgtaat ataatacatc
    14401 attccgggca tatgcttata aaatgttgca tccatggcca ttgagttggc aaatacatct
    14461 attccgactc tgagcataag aactgataat cctgttataa tcgatgaaga aataatcgga
    14521 atggtatgaa aataaagcat gagaaaagtg aatgcaacaa cccctatgct tattctgaaa
    14581 tccgtaccaa agggatagaa attcacttgc cccatgagcg cagtgcacat acctactata
    14641 agcatttctt tatatttgtt tctcaaagca gcaacctcat tacctgattt caattttttt
    14701 atatatttaa ctctgaatat gcatgatttt attgtaatta tatcatatta tggtatgggg
    14761 aacaaaatat gtgtatttct tcctctgctg ctgaactgcg gaaatatttc ttttatattt
    14821 atttccccgt aataatactg tataatatta tttaatgtac tgtaaataag tacatttcca
    14881 aaggaggctg aaacattgaa aaataaagta ataaacctcg cactaactat tatatcagtg
    14941 tcctcccttc tcttcattct tacaacatcg gtatttgcgg attttggaga cagcaccctc
    15001 aataaaggca ttacccatcc cgatgtccat gtccttcagg aaaaactgaa tacactcggc
    15061 tactttaata acgggcagtt cactgattat ttcggggaca agacatataa tgcactgata
    15121 gcttttcaga gggatgaagg actgaatccg gatggcattg caggcaatga gacatttaag
    15181 cgcctcagcc tgaaaatcac acaggcggaa atacttccta aagactttgt gccaataaaa
    15241 gaaggcgaca taggtgataa ggttgttgat atacaaaaaa agctgaggga tctgaatata
    15301 tataaaactg aaataaactc cttttatgac cagactacca tagaagccgt aataagcttc
    15361 caggcagcaa acgcacttcc ccggaccggt attgtcgaca gtgcgacaat aatcaagctt
    15421 aatactgctt acaacagcat tcttgccgac agggcaagtg caagccgcgc tctgttgaat
    15481 tcaaaggtag ttgaatatgc aaagcaattc ctgggtaagc cttatgtatg gggggcttct
    15541 tccggcaaag cttttgactg ctcaggcttc acagtatata ttatgaagaa attcaatgtc
    15601 agcttgcctc gtaccgcttc cggccagatc aacagcggaa ccaaagtcgg aaaagacgat
    15661 cttcaggcag gagatttggt tttcttcact acatataaaa aaggccccag ccatgtggga
    15721 atatacatag gcgggaacaa gttcatacat gcaagctcag gagttgataa ggtcaccatt
    15781 accgatcttg attccagcta ctataagagt agatacttgg gtgcaagaag gtattcattg
    15841 acagacacca aatagaaaca gcttcgcatg gctgtttcta tttcattttg catattattc
    15901 gataaacttg tatatccaat ttattctttt acttagtgct ttttttggta ttatacaggt
    15961 agaagacata ttttgatatt tttcttaatt aaagtatttg gggtgaagaa atgagtatta
    16021 actctacatc caagagtaaa cagaaagaac tgataaaaaa gctcagaagc agcaatatac
    16081 ttctcaagag gtacagcatg attacagatt ccattatgaa aataacaagt gaagtatttt
    16141 cctccggcga aattgattat atacttcaaa caataatgga taaggcaatt gagataatac
    16201 caggtgctca gagaggcagt atactgatat ataacggcag cgttttggaa tttaaggctt
    16261 cccggggata tgattccagt gcgctggaaa atctgaagct tagtatcgaa gaggtatttc
    16321 aatataattc ggaagacttt tttgaacctt gcatagtaag caatcaggaa cgcttcaata
    16381 gggaacatct tccggattat aaatacgaaa tattgcaaaa gggtttgggc attaatccaa
    16441 aatcgactct cagctgcgca tttcaggtag acaataaatt tcagggaatc ataaacctgg
    16501 ataatatgga tgataatacg gctttcagaa aagaagacaa accattgatt aagcacttgg
    16561 cggttcagat aggaattgcc cttaaaaacg ccagacaaat agagaaaatc ctatttcttt
    16621 caagacatga cagtttgaca ggtatataca accgctgcta ctttgaggag cttctgatgc
    16681 atatcatgca aaccagcaag gcttccgcct cggtcttttc cctggcaatg ctggatataa
    16741 ataatcttaa gtatattaat gacacctatg gtcatgaagc cggtgactta atgctcaaaa
    16801 aattcgccca gtgtgtaaat gaaatcataa cgggcgatga tatattcgcc agatatggcg
    16861 gagatgaatt tgcaattatc tttaaagata atgataacat tcaagcagca aataagatta
    16921 acaacataag gcaaacttta ttgaatttgc cctttacata ctgcggtaaa actattgccc
    16981 cgataggctt cggatgcgga attgtgcaat ttcctgcaga tggatcagaa tttcaagatt
    17041 ttatgaggct tgcagatata agtatgtacc aagataaaaa gaaatcaaaa aaacctgtta
    17101 tatagcaggt tttttgattt ttgatgattt atttcattaa taattctatt tcaaaatcac
    17161 agctgggaca tacacctata tactttccat tgacattgaa cagttcaaaa gcttcattgc
    17221 atttactgca cttcgccaca cactttttta tataaataag ggtgccgata tatttgacct
    17281 tatcctcttt atatttatca actgtctttt catacgcctt gctttcgctt ttatcgtact
    17341 gcaattcatt gcgaagcgtt acaaacttta tattttcgtc ttgcagggat ttcctttttg
    17401 tttccaattc tttaatctct tcagctattt caaaaatcct ttcctgattg tagttaattt
    17461 tctggctgag ttctgtcaaa atgtctatat tcttatgata attgaagtat aagcgaaaag
    17521 cttccatatt gttttcttta tgattgaaaa cattatggca gaacctgcac ttatcagttt
    17581 cgggtatatc aaagctttga acatatgtat tccccaagct gtcacaatat ggacaaacaa
    17641 ttatatatat cctgtctaca tagttccctt tttccaattc ggtaaaaagc ctgtcatatt
    17701 catcatttgt gagatcaagc tgttttattg cattctcata aagcacatca ttctcttcgt
    17761 aagtagccag aagctcgatg aatttgtcgg ctttatactg cggaatcacc ttttgctgct
    17821 taaggtactc aataatgttc attttattcc ctccccgcta tgatataatt actcgccccc
    17881 attcaaaacc aagcacactg attatcttat tctataaata atcaaaatat ccttttatta
    17941 aaaactgatt attcaaaaaa tatgaagaat gcg
//
LOCUS       DAOQUR010000044        17314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3763_length_17314_cov_10.107133, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000044 DAOQUR010000000
VERSION     DAOQUR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3763_length_17314_cov_10.107133"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1743)
                     /locus_tag="PKI50_09165"
     CDS             complement(208..1743)
                     /locus_tag="PKI50_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNT03909.1"
                     /translation="MYFDNKVWIAKGDNPVYLLPNMANRHGLIAGATGTGKTITLKVM
                     AESFSDMGVPVFLTDIKGDLAGLSEQGSDNPNMAERIAKLGLEGFNYKSYPVCYWDVF
                     GKGGHPVRTTVSEMGALLLSRLLGLNDTQAGVLNIVFRVADENGMLLLDMKDLRAMLS
                     YVGENAKEYTLKYGNVSSQSIGAIQRSLLVLEDQGGAQFFGEPALDIYDWMRTDSEGQ
                     GYINILHCVELFHSPALYSTFLLWMLSELFETLPETGDVSKPKLVFFFDEAHLLFDDT
                     PKALLQKIEQVVRLIRSKGVGVYFITQNPMDLPQEVLGQLGNRVQHALRAYTPAEQKT
                     VKAAAETFRTNPAFDTETAITELGTGEALVSCLDSKGIPGMVERAFILPPQSKFGTID
                     DFTRLRIINSSSMSSKYDTEVDRESAYEMLQAKAMREQMAEKEEKEYTERQKQLEKEE
                     KERQKQLEAERKERERIKRKEETRKYSRKTLAERAADNIVGTVGREVGRTLIRGILGS
                     LKR"
     gene            complement(1997..2746)
                     /locus_tag="PKI50_09170"
     CDS             complement(1997..2746)
                     /locus_tag="PKI50_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNT03910.1"
                     /translation="MEAVKRFDVRKLVIVGVLGGVSAILGMTPLGFIPVGPTRATIMH
                     IPVIIGAIMEGPIVGALVGLIFGLFSVFQALTNPTPVSFVFLNPLVSIVPRVLIGITS
                     YYVYTALSNLGNRKTLGILNVIWIAIIGYLSYGIYKNIENSQSPWLIAVNIALIAFTA
                     AIAFLANRKFRGKALDLVISAIVGTMTNTVLVLSMIYLIYAESFVKSIGQDPALARKI
                     IMGIGIANGIPETIIAILIVTSVVGALKRAE"
     gene            complement(2921..3877)
                     /locus_tag="PKI50_09175"
     CDS             complement(2921..3877)
                     /locus_tag="PKI50_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNT03911.1"
                     /translation="MYPGYITDVKGIIVGHSQSEKGMTGCTAVICEKGAVGGVDVRGS
                     APGTRETDLLKPDKLVDRIHAVVLSGGSAFGLEAASGVMRYLEEQGIGFDVGVAKVPI
                     VASAVIFDLNIGDHRIRPDLNMGYDAARSASAEEGRQGNAGCGLGATVGKAFGPEHAM
                     KSGLGSASLKSGELIVSAIVSANSFGDIFDYETGRQLAGVYDYEKREMLNTYEIMKRE
                     GRVLGFPMRNTTIGVVAANAVLTKAEANKIAAMAHNGFARSINPVHTMMDGDTIFTMA
                     TNEIKADINLVGTMAAEAVSRAVTNAVVYAKSCHGLIACCDI"
     gene            complement(3927..4757)
                     /locus_tag="PKI50_09180"
     CDS             complement(3927..4757)
                     /locus_tag="PKI50_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03912.1"
                     /translation="MGRPWREEYKGGIYHVIARGNNKEYIFRESIDKGYFLKQLKDYS
                     VGMEYRIYGYVLMDNHYHIIIQTYNKKLQEIMHQINNKYSKYFNYKYERVGHVFQGRY
                     KAVLVQDERYMLSLLRYIHQNPIKAHICDSVEKYKWSSDVFYRNSINSYIHIEMIMDM
                     LSKDRKEAIKKYKEYMEQDEEIDYDNSKAIGEEAYQIMCLTRKQVKERKRLDEILIGT
                     GVSEEGYWLIKAGKRRMDLTDYKVKYAQEALSHKYSYKEIGDNIDMSDTGIRNLLKRN
                     "
     gene            complement(4847..5200)
                     /locus_tag="PKI50_09185"
     CDS             complement(4847..5200)
                     /locus_tag="PKI50_09185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNT03913.1"
                     /translation="MVLRRGDIFYGDLSPVIGSEQGGVRPVLIIQNDIGNKYSPTVIV
                     AAITSQINKAKLPTHVELEALKYGITKDSVILLEQIRTIDKRRLKEKIGHLSEELMKQ
                     VDTAIQISFGLIEYT"
     gene            complement(5202..5477)
                     /locus_tag="PKI50_09190"
     CDS             complement(5202..5477)
                     /locus_tag="PKI50_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNT03914.1"
                     /translation="MADLKKIAVCMPDTLLKEIDDMVCHKCINRSEFIRDAMRFYISE
                     IKRSNAVEKLRNGYLEMSEINVTLAEIGLTADYETIIRYETRLAECE"
     gene            complement(5671..6846)
                     /gene="alr"
                     /locus_tag="PKI50_09195"
     CDS             complement(5671..6846)
                     /gene="alr"
                     /locus_tag="PKI50_09195"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT03915.1"
                     /translation="MYDLKEIRAAWAEVDLDALAHNMREIRRLARKDALVTAVIKADG
                     YGHGATKIAQTLLDNGADRFAIAVLDEGIELRNAGFGVPILILGFTDKERAEEIVSFN
                     LEQAVYSWELAEAISKAAVKQNKTAKIHIKVDTGMGRIGLKPDKDSVQLIKKISQLPN
                     IVIEGIFTHFAVADALDKAYTEEQYAKFTWICKELERENVRINVKHCGNSATIIDLPE
                     MHLNMVRAGIILYGLKPSDEVMLDRIELKQVMSLKVRISHVKEIEAGQSVSYGRRFIA
                     EKKSKIASLPIGYADGYTRMLSGKAEALVKGRRVPVVGRICMDQCMVDVTGIEDVKVG
                     DEAVLFGRQGEGFIHIDELAEKLDTINYEIVCMISRRVPRVYVKDGKIVDVLNYLVD"
     gene            complement(6755..7501)
                     /locus_tag="PKI50_09200"
     CDS             complement(6755..7501)
                     /locus_tag="PKI50_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03916.1"
                     /translation="MYRRIICVLIIVCAGLTGCGMIKQKKNFGEAGRRLLKLEAYTCD
                     VVMKVTNNRSTMEYRLKHSYKSPDKYRVEVLAPRELQGQVTIYNGHSSYIYHPGINQY
                     FVTEDFSGSVEYNSFIGSFMEHIRKTDEIKVSYEKYGDKECIVAEFEIPEPNRYMHFE
                     KLWIDRSEIVPLRAEIYGSDGKTYVEIFYDNFVYNPRLEDGDFEIIRKNSMKLQEMKR
                     NVRSEGNQGRMGGSGFGCSCPQYEGNKEAC"
     gene            complement(7565..8011)
                     /locus_tag="PKI50_09205"
     CDS             complement(7565..8011)
                     /locus_tag="PKI50_09205"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNT03917.1"
                     /translation="MIFGIGTDIVEIKRIKKAISRSPRFIERLFTAQELEFYKKKDMK
                     AQHIAGGFSAKEAVLKALGTGLRGFRWKDIEILRGPVGKPIVRFEGNVKQFIEDNGIG
                     IIHVTISHSREFATATAVAEVSMEREGMVIEDLFFQSHEEARKNIH"
     gene            complement(8161..8760)
                     /locus_tag="PKI50_09210"
     CDS             complement(8161..8760)
                     /locus_tag="PKI50_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03918.1"
                     /translation="MGKDISFKDNIVRRNKIPILIYVPEWIQLFSGNKSRSMQKIIAR
                     LEALIARNKECDSELYNLEKRKKMVMNKILYLSKDINENNDKTALSKMEEAEKEIKEI
                     NRKISVLLEELESIPKQINDMNTQLLKETIKRAYELINESRSESEKCQEQVNDIRQKL
                     AELIQKKADMEERVNKLYLFIHGMIGADEMESLDESFLP"
     gene            complement(8870..10546)
                     /locus_tag="PKI50_09215"
     CDS             complement(8870..10546)
                     /locus_tag="PKI50_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT03919.1"
                     /translation="MSKKVVIVGGVAGGASTAARLRRIDERAEIILFEKGQYISFANC
                     GLPYYIGGTIKERKKLLVQTAEAMEARFNIDVRELSEVKRIDRQRKVVEVKNLKDGRN
                     YEENYDVLVLSPGAVPVKPPIPGVDSRNIFTLRDIPDTDSIKEFLNEKKPKNAVIAGG
                     GFIGLEMAESLHSAGLKVDIVVAHEQVMSQLDYSMAAIVHNHIRSKGCGLHIKDGVKA
                     FEPEGDKTVAVLQSGKRIPADMIILSKGVRPNNSLAKEAGLEIGVTGGIKVNEYLQTS
                     DPNIYALGDAIEVTDYVSGKPALIALAGPANKQGRIVANNIAGRRESYKGTQGTAIAK
                     VFDIVAASTGVNEKALKKLGKELGKDYITTTTHSASHAGYYPGGKLMAVKIIYTPEGR
                     VLGAQIVGSDGVDKRIDYIAIAIRHRMTVHDMQEFELAYAPPFSSAKDPVNMAGYVGS
                     NVLNGDVKTAYWYEAMNADISRTFLLDVREPSEYQKGHIPNAVNIPLDQIRSRLKEIP
                     RDKEIIVNCQVGLRSYIGARIMMQNGFDNVRNLSGGYKTYSAVEADMKSI"
     gene            10841..11545
                     /locus_tag="PKI50_09220"
     CDS             10841..11545
                     /locus_tag="PKI50_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNT03920.1"
                     /translation="MSNISLILAFSAGLLSFLSPCVLPLVPAYITYITGSAIADLNTG
                     RARLYALYKSLGFVLGFSLVFIAMGASITSIGRLFAGNMNTFRKIGGTLIVLFGIHIT
                     GLFKIKTLYYEKRLVPFEKLSRNISSVFIGMAFAAGWTPCVGPILASILIYAGSMETV
                     TMGILLLAAYSLGLAVPFILTAYAVGSFSGYFKRISGYLNTISIISGVLLILMGILIF
                     TNKIAALSRYFDFFNL"
     gene            11563..12177
                     /locus_tag="PKI50_09225"
     CDS             11563..12177
                     /locus_tag="PKI50_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT03921.1"
                     /translation="MKKNIIIGITAIILVATAVYVTKNYNSKSEIVINPPRQDQQADQ
                     SASGPDSDPAPSSDSEGNSGESKINLMENYDFTLKDLEGNEVKLSSLKGNKIFLNFWA
                     TWCPPCKAEMPDIQKLYQETKDSGLVILAINVDTDKKAAQDFIDANNYNFTVLWDENG
                     EVSRLYQVTAIPTSYFIDTEGFLDAGTTGAISLEAMKEYVNNLE"
     gene            12221..12742
                     /locus_tag="PKI50_09230"
     CDS             12221..12742
                     /locus_tag="PKI50_09230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT03922.1"
                     /translation="MNNNPTAIIEMENGDVMKLELYPDIAPNTVRNFISLANKGFYDG
                     VIFHRVIPGFMIQGGEPQGVGVGGPGYSIKGEFKANGFENNLKHTKGVISMARSMSPN
                     SAGSQFFIMVADSPHLDGQYAAFGKVTEGIETAEKIAAVKRNRSDKPLEDQRIKKVAA
                     DTKGIEYGEPEKQ"
     gene            complement(12777..13682)
                     /locus_tag="PKI50_09235"
     CDS             complement(12777..13682)
                     /locus_tag="PKI50_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT03923.1"
                     /translation="MMKTRRRYFGGGAEANDFWPSFTDVMSTLALILFFLMLLAYVQN
                     IIFGNNLENAKQQLEISRAEISSAERELRRIRLEVDKTKTALALSEQEIENQKAIIAL
                     SNEELESLRVRLQGIAFLRLDILQKVKESVEQELGRYNDRGQELVSIGDNANIIINES
                     LVFAYNSYKLKPEAEDLLTQLAKAFERVLENPEVRENIDAISIDGHADSMGGSAYNRE
                     LSARRATEVINYMFKVNPNLEDKYGEYFAANGFSEFRPISTGNDEESRAANRRIEISI
                     IVKDSNIQKVIEKYLNETEEIFEER"
     gene            complement(13679..14731)
                     /locus_tag="PKI50_09240"
     CDS             complement(13679..14731)
                     /locus_tag="PKI50_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03924.1"
                     /translation="MEILTKSISNLGYIAYSIICLIAIIFVVSFITMVFVRGKYKGLQ
                     NELSSAAERRDARFRSHVLSDIVEDYKTISLASKGEINTQAIVEKNLSIKLRGLGLGE
                     RFVKNSVSLMVVLGLLGTFYGLTMSVGRLVELLGTSGNSDILVSMDSVVTGLISSVRG
                     MAVAFSTSLAGVGGSIVITVLGIVINIEESRQSLMVQLEDYLDNTVEQELLPKKESEL
                     SRISRVIESSFDGFSSRVEDILRSTVLDFSDKLAAASSNIENSARCLDETIIKFEKAL
                     SVFSSNTRDFSEFNHNLRGNIERMDVSFINLRESIAETSKNIASNQEAMNSFSAAVQQ
                     AAAVIGSEKGTGSGGR"
     gene            15071..16429
                     /locus_tag="PKI50_09245"
     CDS             15071..16429
                     /locus_tag="PKI50_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNT03925.1"
                     /translation="MDKIMELNGIINGFVWGPYMLIFLVGTGVYLTFRTNFMSITKLG
                     YILKSTLFKMFKKGGEGEGEITAFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WVSAIFGMTTKFGEVVLSINYREKTPDGRFVGGPMYYIEKGLKMKWLAYVFAAFGAIA
                     AFGIGNMVQSNSVAASLEVSFGVNKLVTGIVLAVLAAVVIIGGIKRIAQVTERLVPFM
                     AAFYILGGLFIIISNISHLGEAFGLIFGSAFTGTAAFGGFAGSTMKIAMTRGIARGVF
                     SNEAGLGSAPIAHAAATTDHPVRQGLWGVFEVFVDTIIICSITALSIIMSGVWSGGET
                     GAALTTAAFESTIPGGGYIVSIGVLLFAFSTILGWAYYGERCAEYLFGPKINMAYRIL
                     WIPFVAIGAIGGLNFLWDLADTLNGLMAIPNLIGVMGCSGIIIKLTKEFFAKQYVEEE
                     KK"
     gene            16618..17235
                     /gene="safA"
                     /locus_tag="PKI50_09250"
     CDS             16618..17235
                     /gene="safA"
                     /locus_tag="PKI50_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SafA/ExsA family spore coat assembly protein"
                     /protein_id="HNT03926.1"
                     /translation="MKKKVTAFIVVLCFTISIMQVFADITYTVRSGDTMWKIAVKHQV
                     GLSEIIAKNPHIKNPALIYPGQKLTIPTTTAINSLENEVIRLTNAERTKRGLPALKAN
                     WQLSRCARYKSQDMINRNYFAHQSPTYGSPFDMIESFGIRMAAGGENIAMGQRTPQEV
                     VNAWMNSPGHRGNILSTAFTEIGVGCAKNKNGVYYWTQMFIRPGR"
BASE COUNT         4615 a         4177 c         3079 g         5443 t
ORIGIN      
        1 agcgattcca tgaaacagcc gtgtctcccg tgttacccgt tacctacgtc gcattctcac
       61 tcgattctgt tacccaaagc caataagtgc ttgctgttat acaaggccag tgctatgtag
      121 gatgaccaca aacttcagat gtgtcgcatt cttctattgt gaaaagagat gtcgctaact
      181 acttaagtag ttttgcgaca tctcttttta tctctttaat gaaccgagta ttcctcgtat
      241 gagagttctt ccgacctctc tgccaacagt tccgactata ttatcggcag ctcgttcagc
      301 taaagtcttt ctgctgtatt tacgggtttc ttcttttctt ttaattcttt ccctctcttt
      361 tcgctctgcc tccaactgct tctgcctctc tttttcttcc ttttccaact gtttctgccg
      421 ttctgtatat tccttctcct ctttttcagc catctgctcc cgcattgcct ttgcctgcag
      481 catctcgtaa gcagattccc tgtccacttc ggtatcgtac ttgctgctca tggatgaaga
      541 attgattatc cgaaggcgag taaaatcatc aatcgttccg aatttactct ggggaggaag
      601 aatgaatgca cgctccacca tacccggaat ccctttggaa tccaggcatg acaccaatgc
      661 ttcccctgtg cccagctctg ttatggcagt ttccgtatcg aaggcaggat tcgtcctgaa
      721 ggtctccgct gctgctttta cggtcttctg ctccgccggc gtataagcac gaagggcatg
      781 ctgtacacgg ttacccagct gtcccagcac ttcttgaggt aaatccatag gattctgtgt
      841 tataaaatat actccgaccc ccttggatct tatcaacctt accacctgct caatcttttg
      901 aagcagtgcc tttggtgtat catcaaacaa taaatgggct tcatcaaaga aaaataccag
      961 cttgggctta ctcacatctc ctgtttcagg taaagtttca aaaagctccg acagcatcca
     1021 cagcaaaaat gtggaataca atgcagggct gtggaaaagc tccacgcaat gcaatatatt
     1081 gatatatccc tgtccttcgg aatcggtcct catccagtca taaatatcca gagcaggctc
     1141 tccaaagaac tgtgctcctc cctgatcttc caggacaagc aggctgcgtt gaattgctcc
     1201 tatactttga gaggagacat tcccatattt caaggtatac tcctttgcgt tttcgcccac
     1261 atagctgagc atagcccgaa gatccttcat gtccaatagg agcatgccgt tctcatccgc
     1321 tacgcggaat actatgttca gcacccctgc ttgtgtatca ttcaggccca gcagccggga
     1381 cagcagcaaa gcccccatct cagaaacggt agtgcgcaca ggatggccgc ccttgccaaa
     1441 tacatcccaa taacatacgg ggtacgattt atagttgaag ccctctaatc ccaacttagc
     1501 tattctttct gccatattgg gattatcgct gccctgctcc gacagtccgg caaggtctcc
     1561 cttgatatcg gtaagaaaga caggcactcc catatcgctg aaagactctg ccatcacttt
     1621 gagagtaatg gtttttcctg taccggttgc tcctgctatc aagccgtgtc gattcgccat
     1681 attgggcagg aggtaaacag gattatcccc tttggctatc catactttat tatcaaagta
     1741 cataaatgcc gctcctttca ctgtattttc aaaaaaatgc gtctcgcgac gctttttttt
     1801 cgctagggaa ccctatggtt cccttcgacg gatgcaagca tcctgagcac cctcctttat
     1861 cggcaggggg cttcaccccc ttgcatcccc cattgtatag aaaagtatat tttcctatac
     1921 aataaaaaag caccttctgt caatatacta acagaaggtg cctcggttgt aaatatatag
     1981 gctcttggca gctaatctat tcggctcttt ttaaagcgcc taccacactg gttacaatca
     2041 atattgcaat aattgtctcc ggtattccgt tcgcaatgcc tatgcccata attattttcc
     2101 ttgccaatgc agggtcttgt cctatactct ttacaaagct ttccgcataa atcaaataaa
     2161 tcatgctcaa aaccagcact gtattggtca ttgttcctac aatagccgat attacaagat
     2221 ccagtgcttt gcccctaaac ttcctgtttg ccaaaaatgc tatcgctgct gtaaaagcta
     2281 tcagcgcaat attcacagct attaaccagg ggctttgaga attttcaata tttttgtaaa
     2341 taccatagct taggtagccg attattgcta tccagatgac attaaggata cctaaggttt
     2401 ttctgtttcc gagattgctc aaagcagtat atacataata ggatgtaata ccgataagca
     2461 cccttggcac tatggaaacc aatggattaa ggaaaacaaa ggataccggt gtgggattgg
     2521 ttaatgcctg gaaaacactg aagagtccaa atataagccc tacaagtgcg ccaactatcg
     2581 ggccttccat aatagcacct attattacag gaatgtgcat tatagttgcc cttgtcggtc
     2641 ctacgggtat gaatcccagg ggcgtcatgc ccagaattgc ggatacacct cccaataccc
     2701 cgacaataac taattttctt acgtcaaatc tctttactgc ttccatttta atacctccgc
     2761 tctagttcca tattttggat accagtcgta aaacataatg tttataacta tatccggtcc
     2821 aactccttac ggatgctggc aacaagaata ttataataaa aataataaaa aaaaacaata
     2881 agttaatttt aaaaacgatt tggagggtat aaacggtata ctatatatca cagcaggcaa
     2941 tcagcccatg gcatgacttt gcatatacaa ctgcattggt tactgcccgt gaaaccgcct
     3001 cggcggccat agtgcccaca aggttgatat ccgcttttat ctcgttggta gccatagtaa
     3061 atattgtatc tccatccatc atggtatgca ccggattgat tgatctggca aaaccgttat
     3121 gcgccatagc cgcaatttta tttgcttcag ctttggttaa tacggcattt gcggcaacca
     3181 ctcctatggt tgtattcctc atgggaaagc ccaacaccct tccttctctt ttcattattt
     3241 catatgtatt cagcatttcc cttttttcat agtcatatac ccctgccagc tgcctgccgg
     3301 tttcatagtc aaaaatatct ccaaagctgt ttgccgatac tattgcagac actatcagtt
     3361 cccctgactt gagagaggcg cttcccagcc cggatttcat ggcatgctcg gggccaaagg
     3421 ctttacctac ggtagcaccc aatccgcagc cggcatttcc ctgtctgcct tcttccgcag
     3481 atgcacttct tgccgcatcg taacccatat tcaaatccgg tctgattctg tggtcaccta
     3541 tgttcaggtc aaaaataact gcagaagcca caataggcac tttagcgacc ccgacatcaa
     3601 atccgattcc ctgctcttcc aaatacctca tcaccccgga agctgcttca aggccgaaag
     3661 cacttcctcc tgacagtact actgcatgaa tcctgtccac cagcttgtcc ggcttcaata
     3721 agtccgtttc cctggttcca ggagcagagc ctctcacgtc cactccccct acggcaccct
     3781 tctcacagat aaccgccgta caaccggtca tgcctttctc ggattgactg tgtcctacta
     3841 taataccctt tacatccgtt atatatcccg gatacatacc tatccctccc tcgattccaa
     3901 tggtgccagg caccaaacaa aagaacttaa tttcttttca ataagtttct tatacccgta
     3961 tctgacatgt ctatgttgtc tcctatctct ttatatgaat atttatgact taaagcttct
     4021 tgggcatatt taactttgta atctgttaaa tccatccgcc gtttcccagc tttaataagc
     4081 caatatcctt cttcactcac acctgttcct attaatattt cgtccaatct tttcctttcc
     4141 tttacctgtt ttcttgtcag gcacattatc tggtacgcct cttctcctat tgctttactg
     4201 ttatcatagt caatctcttc atcctgctcc atgtactctt tgtatttttt gattgcttct
     4261 ttcctgtcct ttgacagcat gtccataatc atttcaatat gtatatagct gtttatgcta
     4321 tttctataaa atacatcact gctccattta tacttttcaa cgctatcaca gatgtgcgcc
     4381 tttattggat tttgatgtat gtatctgaga agagataaca tatacctttc atcttgtacc
     4441 aatacagctt tgtatcttcc ctggaaaacg tgtcctaccc tctcatattt gtaattgaag
     4501 tacttactat acttgttatt aatctggtgc attatctcct gcagcttctt attatacgtc
     4561 tggattataa tatggtaatg attatccatc aagacatacc catatatcct gtactccatt
     4621 cctacgctat aatctttcaa ctgtttcaaa aaataacctt tatcaatact ttctctgaaa
     4681 atgtactctt tattatttcc tcttgctatt acgtggtata ttccgccctt atattcttct
     4741 ctccacggcc tgcccataaa aaagcacctc ctaatataat actatcagaa ggtgttccat
     4801 atgtaaatat ctgccttatt ttgttctttt gttcggtgcc tggcacctat gtatattcaa
     4861 ttaatccgaa gcttatctga attgctgtat ccacctgttt cataagctcc tccgacaaat
     4921 ggccgatttt ttccttcagc cttctcttgt ctattgtccg tatttgttcc aataatatga
     4981 ccgagtcctt tgttatgccg tatttcaaag cttccagctc aacatgggtg ggcagctttg
     5041 ctttattaat ctgtgaagtt attgcagcaa caataacagt gggactatat ttgtttccta
     5101 tatcattctg tattatcaat acgggtctta caccgccctg ttccgatcct atgaccgggc
     5161 ttaaatctcc atagaaaata tcccctcttc ttaaaaccac attattcaca ctccgcaagc
     5221 cttgtttcat atcttattat tgtttcatag tctgccgtaa gtcctatttc tgctagtgtt
     5281 acatttattt ctgacatttc aagatatccg ttccttaact tttctactgc attagacctc
     5341 tttatctcac ttatataaaa acgcatggcg tctctgatga attcacttct attaatacat
     5401 ttatgacata ccatatcatc aatttctttt aaaagcgtgt ccggcatgca aacggcaatc
     5461 ttttttaaat cagccacact agcacctcct aagttttttg aagaccacac agagtataca
     5521 acattctgca tcgtcaataa tatgtatgag caaggagtgt aaaattatgt gtcggaaatg
     5581 cacattatac catatatgat tatactaacc gcatatacaa tattatatac caattcttgc
     5641 actatgccta tgtacaatat atggcagcat ctagtctact aaataattca acacatcgac
     5701 tatctttccg tccttgacat atactctcgg aactctcctg cttatcatgc atacaatttc
     5761 ataatttatg gtatcaagct tttctgcaag ttcatcaata tgaataaagc cttctccctg
     5821 tctcccgaaa agcaccgcct catcaccgac ttttacatcc tcgatacctg ttacatcaac
     5881 catgcattgg tccatgcata ttcttccaac cacaggtact ctccggcctt ttaccaatgc
     5941 ctcagctttg cctgacagca ttcttgtata gccatcggca tatccgatag gcagcgacgc
     6001 gattttgctc tttttttctg caatgaacct tcttccgtaa cttacgctct gtccggcttc
     6061 tatctccttg acatgtgaga ttctgacctt caatgacata acctgtttaa gctcaatcct
     6121 atcaagcatt acctcatccg aaggcttcag gccatagaga attattcccg ccctgaccat
     6181 attcaaatgc atttcaggca ggtcaattat tgtagcactg tttccgcagt gttttacatt
     6241 tattctgaca ttctcccttt caagctcctt gcatatccac gtgaatttcg catattgttc
     6301 ttcagtatat gctttatcca aggcatcagc caccgcaaaa tgtgtaaata tgccttcaat
     6361 aacaatattg ggcagttgac taatcttctt tataagctgt acggagtcct tgtcgggctt
     6421 taagcctatc cttcccattc cggtatcaac ttttatatgg atttttgctg ttttattctg
     6481 cttcacagcc gcctttgaaa tagcttccgc cagttcccat gaatacacag cctgctccaa
     6541 attgaagctt actatttcct ctgccctttc cttgtcggta aagccgagga ttagaatagg
     6601 cactccaaag cctgcattcc gtagttcgat accctcatcc aatacagcaa tggcaaatct
     6661 gtctgctcca ttgtccaaaa gggtttgtgc tatttttgtt gctccatgtc cgtatccgtc
     6721 agctttaatt actgcggtta caagtgcatc tttcctagca agcctcctta tttccctcat
     6781 attgtgggca agagcatcca aatccacttc cgcccatgcg gccctgattt ccttcagatc
     6841 gtacatttct tttcatctcc tgtaatttca tagaattttt tcgtatgatt tcaaaatccc
     6901 cgtcttccaa tctgggattg tagacaaaat tatcataaaa tatctctacg tatgttttgc
     6961 cgtcacttcc gtatatttcc gctcttaggg gcacaatttc cgacctgtct atccaaagct
     7021 tttcaaaatg catgtatctg ttcggctcag gaatctcaaa ttctgcaaca atgcactcct
     7081 tatccccata cttttcatag ctgactttta tttcatcggt ttttctgata tgttccataa
     7141 atgaacctat gaagctgtta tactccactg accctgagaa atcttcagtt acaaaatact
     7201 gatttattcc gggatgatat atataagagc tgtgtccgtt atatatagtc acttgtcctt
     7261 gaagctccct cggtgccaaa acttctaccc tgtacttgtc cggactttta taggaatgct
     7321 tcagcctgta ttccattgta cttctgttgt ttgtgacctt cataacaaca tcgcaggtat
     7381 aggcctccag cttcaagagc ctcctgccgg cttccccgaa gttctttttc tgctttatca
     7441 ttccgcagcc ggttaaacct gcacatacaa taatgagcac gcaaataatc ctacgataca
     7501 caatataccc tcctgttact ccataccgta ttatttatac attattgtat gtgaaatttt
     7561 tttattaatg aatattcttt cttgcctctt catggctttg aaaaaataaa tcctcaataa
     7621 ccatgccttc cctttccatg gaaacttccg ccactgcggt agctgtagca aattccctgg
     7681 aatgggatat ggttacatgt attatcccga ttccgttatc ctctataaac tgtttgacat
     7741 tgccctcaaa acggactatg ggctttccga ccgggcctct caggatttcg atatccttcc
     7801 atctaaaacc tcttaatccc gttcccagcg ccttaagcac agcttccttt gcagaaaaac
     7861 ctcccgcaat atgctgtgct ttcatatctt ttttcttgta aaattccagc tcctgtgcgg
     7921 taaacaatct ttcaataaat ctcgggcttc tggatattgc cttcttaatt ctttttattt
     7981 caacaatatc agtcccaata ccgaatatca ttatatcact cccgtatttc agcacattca
     8041 taatgcactt taattgtata tattctacaa taaaatccta tatcctctca ttaaccgtat
     8101 ataataataa aaaaacaaag gtcaattccg ccgctgcagc agaatcaacc ctatatgctc
     8161 ctatggcaaa aagctttcat ccaggctttc catctcgtcc gctccaatca ttccatgaat
     8221 aaataaatac agcttgttta ccctttcctc catatccgcc tttttctgta tcaattccgc
     8281 cagcttctgt ctgatatcgt tcacctgctc ctggcacttc tccgactccg aacggctctc
     8341 atttattagc tcataggccc tcttgatagt ttccttcaga agctgcgtat tcatatcatt
     8401 tatttgttta gggatagatt ccagttcttc taaaagtacc gaaatcttcc tgttaatttc
     8461 ttttatttct ttctccgctt cctccatttt ggataaggcg gtcttgtcgt tattttcatt
     8521 tatatccttt gaaagatata gtattttatt cataaccatt ttttttcttt tttccagatt
     8581 atataactcg gaatcacatt ccttattcct ggcaataagc gcttccaatc tggctataat
     8641 tttttgcatg cttctggact tgtttccgga aaatagctgt atccactccg gtacgtagat
     8701 gagtatcgga attttatttc ttcttacaat attgtcttta aaactgatgt ctttgcccat
     8761 aggtattctc tccatttcgt acagcatatc catccgcata tcatgaagaa cactataaat
     8821 tgccaaaaat tattttatta aattaatttt actataaaaa caaatatagt caaatgcttt
     8881 tcatatctgc ttccactgcg ctgtatgttt tatagccgcc gctcagattt cttacattat
     8941 cgaaaccgtt ttgcatcatt atccttgctc ctatataaga acgcaagccc acctggcaat
     9001 tgacaataat ctctttgtcc ctgggtatct ccttgagcct gcttcttatt tgatccaggg
     9061 gaatattcac cgcatttggg atatgcccct tctgatattc ggaaggttcc cgtacatcca
     9121 aaagaaatgt ccggcttata tccgcattca ttgcctcata ccagtaagcc gtcttgacat
     9181 ccccgttcaa aacattgctt ccgacataac cggccatgtt tacaggatcc tttgcggatg
     9241 agaagggcgg agcataagca agctcaaatt cctgcatgtc atgcactgtc atcctatgcc
     9301 ggattgctat tgctatataa tctattctct tatccacccc gtcagaaccg actatctgtg
     9361 cccccagcac tctgccttca ggagtataga tgatttttac cgccattaat tttcctcccg
     9421 gataatagcc cgcatgggaa gcagagtgtg tagtagttgt gatatagtcc tttcccaatt
     9481 cctttccaag ctttttaagg gccttctcat taactcccgt tgaagctgca acaatatcaa
     9541 atacttttgc gattgccgtt ccctgtgtcc ccttatagct ttccctgcgt cctgcaatat
     9601 tgtttgccac tattcttccc tgtttgtttg caggcccggc aagagctatc agcgccggtt
     9661 tgccgcttac ataatctgtt acttctatag catcacccag agcatatata tttggatcag
     9721 aggtctggag atactcattt acctttatac caccggttac acctatttca agtccagcct
     9781 ccttagccag ggagttattt ggtcttactc cttttgaaag tattatcata tctgccggta
     9841 tccgctttcc gctctgcagt actgcaactg tcttatcgcc ctccggctca aaggccttta
     9901 ccccatcctt tatgtgaagt ccgcagccct tgcttcttat atgattatga actatggcag
     9961 ccatagaata atccagctgc gacataactt gttcatgggc tactactatg tcaactttta
    10021 aaccggctga atgaaggctc tccgccatct cgagaccaat aaaacctcct cctgcgataa
    10081 ctgcgttctt aggttttttc tcatttaaaa attcttttat ggaatcagta tcaggaatat
    10141 ccctcagggt aaatatgttg cggctgtcaa ctcccggaat aggaggcttg accggaacag
    10201 caccagggga gagtaccaga acatcatagt tttcttcata attccttcca tcctttagat
    10261 tctttacttc aactaccttt ctctgccggt ctatcctttt aacctctgaa agttctctga
    10321 catctatatt aaagcgagcc tccatagcct ctgctgtctg aaccagcagt ttctttcttt
    10381 cctttattgt tccgccgata taataaggaa ggccacaatt ggcgaaggat atgtactggc
    10441 ccttttcaaa tagtattatc tcagcccttt catcaatcct cctgagcctt gctgcagtgg
    10501 atgctccacc tgcaacgcct cccactataa ctaccttctt actcatatcc attcctcccc
    10561 tgtcttttgt attggttatg tattcatttc aacattattt atatatttgt tatccctata
    10621 ttatgtaaag gttcataatg attatatttt tatatccttc agctgtcaag aatccatgta
    10681 atcggctatt gagtcaaaat ccatataaat actttacatt atcggcatta tataatataa
    10741 taaatatata cccatggggg gtatgatttt gattcgaact ttggataaca gacactaaag
    10801 tgtaacaagt aataagattt aatcaaggca ggtgattaaa ttgagcaaca tatcacttat
    10861 actggctttt tcggcagggc ttctgtcctt tctttcccca tgcgtgctgc ctctggtacc
    10921 cgcctatata acttatatta ccgggtctgc tattgctgat cttaataccg gaagggcaag
    10981 actttatgcc ctgtataaat ccctggggtt tgttctgggc ttttctctgg tattcatagc
    11041 aatgggagcc tcaataacct ccataggcag gctttttgcc ggaaacatga atactttcag
    11101 gaaaatcggc ggtactctga tagtactgtt tggaatacac attacagggc tttttaaaat
    11161 aaagacattg tattacgaaa aaaggcttgt gccttttgaa aagctgagca gaaatataag
    11221 ctcggtattt ataggaatgg ccttcgcagc cggctggact ccctgcgtag gccctatact
    11281 ggcatcgatt ctcatatatg ccggcagcat ggaaacagta accatgggga tactgctgct
    11341 tgcggcatat tctttagggc tggcggtacc atttatacta actgcttatg ctgtcggaag
    11401 cttcagcggg tatttcaaga gaatatcagg gtacttgaat accatatcga tcataagcgg
    11461 agttttgctc atattaatgg gtatactcat atttacaaac aaaattgcgg cgctaagccg
    11521 gtattttgat ttttttaatc tttaatcaga ggagaattgt ttatgaaaaa aaatattatt
    11581 attgggatta cagctattat tcttgtagct acggctgttt atgtaactaa aaattacaat
    11641 tccaaatccg aaattgtaat aaaccctccc cggcaggatc aacaggcaga tcaatcagcc
    11701 tccggccctg actctgaccc tgcaccttct tccgattcgg agggcaatag cggtgaatca
    11761 aagataaatt tgatggaaaa ctatgatttt acccttaagg acttagaggg caatgaagta
    11821 aaactcagca gccttaaggg caataaaata ttcctcaatt tctgggctac ctggtgcccg
    11881 ccctgcaagg cggaaatgcc ggatatacag aagctttacc aggagacaaa ggacagcggc
    11941 cttgtaatat tggcaataaa tgtcgataca gataaaaaag ccgcacagga ctttattgat
    12001 gccaacaact ataacttcac cgtcctgtgg gatgaaaacg gagaggtttc ccggctatat
    12061 caggtcacag caatacctac ttcatatttt atcgataccg aaggcttcct tgacgccgga
    12121 accacaggtg cgatatccct ggaagcaatg aaggaatatg tcaataatct ggaataagcc
    12181 tttaaatact tcagaacaaa cagaaagagg atgatagtat atgaataaca accccacagc
    12241 aattatagag atggaaaacg gagatgtgat gaagctggaa ctgtatcccg atatcgcccc
    12301 caatacggta aggaatttta tatcccttgc aaataaaggc ttttatgacg gagtcatatt
    12361 ccacagggtt atccccggtt ttatgataca ggggggagag ccccagggtg tgggtgtcgg
    12421 cggtcccggg tacagcatca agggcgaatt taaagcaaac ggctttgaaa ataacctgaa
    12481 acatactaag ggtgttatat caatggcaag atcaatgtcc cccaattcgg caggctcaca
    12541 gtttttcata atggtcgcag attcacctca cctggatggc cagtatgccg cttttgggaa
    12601 agtaacagaa ggtattgaga cagcggaaaa aattgcggca gtgaaaagaa accgctctga
    12661 caaacccctt gaggatcaga gaataaagaa ggttgcggca gatactaaag gtatagaata
    12721 cggtgaaccg gaaaagcaat gaggctgatg acaaagtcat cagcctcatt cttccgctat
    12781 ctttcttcaa atatttcctc tgtttcattc agatacttct caataacctt ttgtatattt
    12841 gaatccttca ctattatgga aatttcaata cgcctgttgg cagccctgct ttcctcgtca
    12901 tttcccgtac ttatggggcg gaattcagaa aaaccgttgg ccgcaaaata ttctccgtac
    12961 ttgtcctcca aattgggatt cactttgaac atgtagttta ttacctctgt agccctcctg
    13021 gcggataatt ccctgttata agccgaacct cccatgctgt cggcatgtcc gtcaatgctt
    13081 atggcatcta tgttttctct tacctcgggg ttttccagca ctctttcaaa ggccttggcc
    13141 aattgtgtca ggaggtcttc cgcctccggc ttaagcttgt atgaattata agcgaaaaca
    13201 aggctttcat taattataat atttgcattg tctcctatag ataccaactc ctggccccta
    13261 tcattatacc tccccaactc ctgctctaca gattctttaa ccttctgcag tatatccagc
    13321 ctcagaaagg ctatgccctg aaggcgtact ctgaggcttt ccagctcttc attgctcaaa
    13381 gcgatgattg ctttctggtt ctcaatttcc tgctcagaca atgccagagc cgtctttgtc
    13441 ttatctacct ccagccttat tcgtctcagc tctctttctg cgctgcttat ttccgcccgt
    13501 gatatctcca gctgctgctt tgcattttca agattgttcc cgaatattat gttctgtaca
    13561 taggccagca gcataagaaa gaacagtatc aatgccaagg tagacattac atctgtgaag
    13621 gacggccaga aatcattggc ctcggctcct cccccaaaat atcttctcct tgttttcatc
    13681 atctgccccc acttcctgtg cctttttcac tgccaataac tgctgccgcc tgctgcacag
    13741 ccgccgagaa actgttcatt gcttcctgat ttgaagcaat attctttgaa gtctccgcaa
    13801 ttgattccct tagatttatg aaggatacat ccattctttc aatattaccc ctcaggttat
    13861 gattgaattc actgaaatct ctggtattgc tgctgaatac cgaaagcgct ttttcaaact
    13921 ttatgattgt ctcatcaagg catcttgcgg aattctcgat attgctgctg gctgccgcaa
    13981 gcttgtccga aaaatccagt actgtgctgc gcagaatatc ctccaccctt gaactgaaac
    14041 cgtcaaaaga ggactcgatg accctgctga tgcggctaag ctccgattcc ttcttcggaa
    14101 gaagctcctg ctccacagta ttatccagat agtcctccaa ttgaaccatg aggctttgtc
    14161 ttgactcctc gatatttatc acaattccca aaaccgtaat cactatagaa cccccaacac
    14221 ccgcaagact tgttgagaaa gctaccgcca tcccccttac tgaagaaata agccctgtta
    14281 caacagaatc catactgaca agtatatcgc tgtttccgct ggtacccagc agttccacca
    14341 gtctcccgac tgacattgta agcccgtaga aggttcccag gagccccagc accaccatca
    14401 atgaaactga gttcttcaca aaccgctctc ccaatcccag ccctctaagc tttatgctta
    14461 aatttttctc tactattgcc tgagtgttga tttctccttt ggaagccaga gatatggttt
    14521 tataatcctc tactatatcg cttaatacat gactcctgaa cctggcatcc cttctttctg
    14581 cagccgacga caattcattc tgcagcccct tgtatttccc cctgacaaac accattgtaa
    14641 taaaggaaac cacaaatatt attgctatta agcatatgat tgaatatgct atatagccca
    14701 agttacttat actctttgtt aaaatttcca agaatcaccc cacctttcaa ttttttgagt
    14761 gccaggattc ttttatcagc ttctatttac tattttatag aaaacgatga aatttatcaa
    14821 taaattaaaa atttaaaaaa ttactgcata atttcttgct tgcattgtat tattatagag
    14881 ataacaagaa attaactgaa aattccaaca attcaatata atgaatattg tataaaaata
    14941 tatattgtaa aatactacta ttattgataa aataaattat acaatattat tattctgagc
    15001 attatatatc tcccctaaat ttgttatttg ggaagatatt taatgtataa aaataacaag
    15061 gaggtattcc atggacaaga taatggaact caacggtatt attaacggtt ttgtatgggg
    15121 cccttacatg ttgatcttct tagttggtac aggcgtttat ctcacttttc gcactaattt
    15181 catgtcaatt acaaaactag gttacatact taagagcact ctctttaaaa tgttcaaaaa
    15241 gggaggagaa ggagaaggcg aaatcaccgc attccaggct gttgcaacag cgcttgcagc
    15301 aacagtcggt acaggtaata tcgccggagt tgctacagct atagccctag gaggtcccgg
    15361 agcagtattc tggatgtggg tatctgctat atttggaatg accactaaat tcggagaagt
    15421 tgtactttcc atcaattaca gggaaaagac accggacggg cgtttcgtcg gcggtcctat
    15481 gtattacatt gaaaagggac ttaagatgaa atggctggca tatgtatttg cagcatttgg
    15541 tgcaattgca gcctttggta taggcaacat ggtccagtca aactccgttg ctgcttccct
    15601 ggaagtgtcc tttggagtca ataagttagt tacaggtatt gtattggcag ttttggcagc
    15661 agtagttata ataggcggta tcaagagaat agctcaggtt actgagagac tggtaccgtt
    15721 tatggcagca ttctatattc ttggcggctt gttcataata atctctaata tttctcattt
    15781 aggagaggct ttcggattaa tattcggaag cgcatttaca ggaactgctg cattcggcgg
    15841 ctttgcaggt tcaactatga agatagccat gacaagaggt attgcccgtg gtgtattctc
    15901 caatgaagca ggtctcggca gcgcacctat agctcatgct gcagctacta cagatcaccc
    15961 tgtacgtcag ggactctggg gagtatttga agtatttgta gatacaataa taatttgttc
    16021 cataactgct ttgtcaatca ttatgtcagg tgtatggagc ggcggcgaaa ccggtgcggc
    16081 attgactacc gcagcatttg aatcgactat acccggcggc ggatacatag tctccatagg
    16141 tgtactgctg ttcgcattct ccacaattct cggctgggca tattacggag agcgctgcgc
    16201 ggaatatctg ttcggaccca agatcaatat ggcatacaga atcctgtgga taccgtttgt
    16261 tgctatcggt gccatcggcg gcttgaactt cctctgggat ttggcagata cactgaacgg
    16321 cttgatggct atcccgaacc ttataggtgt gatgggttgc agtggaatta ttatcaagct
    16381 taccaaggaa ttctttgcaa aacagtatgt agaagaagaa aagaaataaa acataataaa
    16441 gtcaaaaaac gagagtttca gactctcgtt tttttattta ttcattttat tgccggcagt
    16501 taaaaatatt ggcggtccat gatgatacta ttaataaatc tatttgcatt ttccaattac
    16561 ataatctgct taacataaaa aagctgaatt catcaaaaat aattgtgagg tgataaaatg
    16621 aaaaagaaag taacagcttt tatagtagta ctgtgcttca caatatctat aatgcaggtt
    16681 tttgcggata taacctatac ggtgcgttcc ggcgatacta tgtggaaaat agcggtaaaa
    16741 caccaggtag gacttagtga aataattgcc aagaaccctc atataaaaaa tccggcattg
    16801 atataccccg gccagaagct tacgataccg acaacaactg caataaattc cttggaaaac
    16861 gaggtaatac gcctgacaaa cgctgagcgt accaaaaggg gattacctgc tctaaaggcc
    16921 aactggcagc tttccagatg cgcaagatac aagtcccagg atatgataaa cagaaattat
    16981 tttgcccatc aatccccgac ctacggctca ccttttgaca tgatagaatc cttcggaata
    17041 aggatggctg cggggggaga aaacattgca atggggcaga gaacgcctca ggaagttgtg
    17101 aacgcatgga tgaattcacc gggccacagg ggaaatatac tcagtacggc ttttactgaa
    17161 ataggcgtag gctgtgctaa aaataaaaat ggagtttatt actggactca gatgttcata
    17221 aggccgggaa gataacggtg aaggggacac ggggacgttt gggacacggg gacgtttctc
    17281 ctgtcccgct ttcagcggga caggagaaac gtcc
//
LOCUS       DAOQUR010000045        16939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3864_length_16939_cov_15.443734, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000045 DAOQUR010000000
VERSION     DAOQUR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16939
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3864_length_16939_cov_15.443734"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PKI50_09255"
     CDS             <1..930
                     /locus_tag="PKI50_09255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT03927.1"
                     /translation="ANEELEYEYDIEFKQGGYLIVASTEKEVNQFKKNIALQNSLGIP
                     SRLLTPKEAKEIVPHMDETKIIAATFNPKDGHLNPFHTTQAYANAAVRLGVEIMKHTT
                     VTGIKVENGKVAGVQTDKGYISTPVVVNAAGGYSQLIAKMVDIDLPIYSERHQILVTE
                     PVAPMQGPMFMSFSLNIYCQQTPHGSFIMGRGDANEPRDLRITSSWHFMEEMAKTCTE
                     LLPPLKELRVVRQWSGLYNISPDRQPIYGPVKEVEGFYLACGYSGHGFMFGPVTGVVM
                     AEAILGETPTIPVHMLDKDRFERGELLLEPSVV"
     gene            complement(990..1541)
                     /locus_tag="PKI50_09260"
     CDS             complement(990..1541)
                     /locus_tag="PKI50_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNT03928.1"
                     /translation="MKKVYVYLAEGFEEIEAVTVVDTLRRAEIDARMISITGKKNVTG
                     AHGIVVSADEIFENTDNMGQDMIVLPGGMPGTRYLGEHNGLRKIIMDFAEKGKPIAAI
                     CAAPSILGKLGLLDKKEAVCYPGFEETLKGAVIGKNIVSHGSNFITSKGPGTAIYFAL
                     YLVEILRDKKTAEELREGMIVQG"
     gene            complement(1639..2544)
                     /gene="rnhC"
                     /locus_tag="PKI50_09265"
     CDS             complement(1639..2544)
                     /gene="rnhC"
                     /locus_tag="PKI50_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNT03929.1"
                     /translation="MNGTKDKYQYYNHLKNILEENGIEISLYEDINYGLQFSIIISGK
                     KHLIRVYENKKNGTRCDLSQIKDEAAYKLIDGLLRTGSLKAEKAEEPQEADAGLIGTD
                     ESGKGDYFGPLVIAGVYADAGMKKKLMETGAADSKTLSDARIRKLAEEIAGICKYSVV
                     IIGNKRYNELYEKIENLNKLLAWGHARVIENLLEETDCSYALSDQFGSPELIKNALLE
                     KGKNINLEQRPRAEENVVVAAASILARNEYVKAMNKLAEKYGMEFPKGSSSMVTEAAK
                     EFVLLHGKEKLSEVAKLHFVITGKI"
     gene            complement(2595..5399)
                     /locus_tag="PKI50_09270"
     CDS             complement(2595..5399)
                     /locus_tag="PKI50_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNT03930.1"
                     /translation="MKGFGRIKTKGITILLVIIVLAIAFFSNIAEYLINYQWFSKLGY
                     QEVFFKKLFTQMQFFIPALFVLFFLFYLYLKSINTHSVRHSGLVLSSVEKKIRTKISV
                     YGSLGLAFVFSLLFVFDIWYDFLIFINKTPFGLADPIFGKDIGYFVFSLPFFNKLYNF
                     LLMIIFAFAAITFLFNAYNFLTTKVPDEKLNIDIRPVGNSKDMYRNILRTASKQLMFL
                     GGLFFLVLAFGFYLRTFDLLYSSRGVAYGASYTDIKITLPAYYIYMGICILTAALLIL
                     NRNKKNIKLIVLGPLLLVVAMIAAGVIYAVVQNMIVAPNELAREEEFLQYNINYTNYA
                     YNLDKVTEKEFSVNQALTREDIEENEVTVNNIPINDYRPAKDIYNQIQGLKSYYQFND
                     MDIDRYMINGIYRQVFISARELQSANIPKQTEARGASWINKYLKYTHGYGVSMSPVNE
                     VTASGQPRLFIRDMPLISDIDIKVDVPQIYFGESTKDFVIVNSREKEFDYPATNINME
                     TEYSGTGGISLNILNRILFAVREGELNLLLSQDINSKSRILINREIIDRVKKIAPFLT
                     YDEDPYIVIYNGKLYWLIDAFTTSSRYPYSERVNSQTSTNYIRNSVKVVIDAYNGTTE
                     FYIADANDPIIKTYSKIFNTLFKPISEIPEGLRSHIRYPQTLFDIQADIYKKYHMKNA
                     REFYNKSDVWDVSTQIYGTGTNQANAELVESSYLIMRLPDSKKEEFLLIVPYTPQGKT
                     NMISWLAVKNDGENYGEIILYSFPSGKIVEGPMQIEGIVSQDTIIGPQLNLLATGGNS
                     QVVRGNMLTIPIEESILYVEPIYVKALNANALPEQKKVIVYFKNKVVMEDTLDEALDR
                     VFPVKEKPGEKTQDEPVPTPGGNIDTSAEKLIKEANDTFEQAMNAQRSGNWAEYGSKI
                     KALEELLKQLSELTNIQGNQ"
     gene            complement(5526..10961)
                     /locus_tag="PKI50_09275"
     CDS             complement(5526..10961)
                     /locus_tag="PKI50_09275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT03931.1"
                     /translation="MRFINTQYKVISISNEDRYGSTVVVEDTRRGRMLKILRIINYQK
                     ETQDFVDYMKLNMFDHRNLSHPNLANFYFFSRILEIDTKPVVANKYYYTYEHFNGVGL
                     FEFINNQNFDMLLDISVQLCSLVKYLHLRGFLFCSVNINDLFVVYEKGKALLKVSKLP
                     YVKGTEKSVMFDRESNYFISPEAFQFGDYSRASDIYLIGALMFYIFTGAKVEVSNFKD
                     ELKKFRNIEDKNKSQVIEIIKKCTSLKPGDRYESVEKIVEDINRRLNKNFNIINKKHI
                     QKLPRHITRLVSREHHFKRVIESAKGFLFENKLQPKAMIIKGSMGTGKSVFIKASGYR
                     LEQEGISVVYNVLKENVVSSFYYIILLIKEIMRVTDKEIIDRYIADISYIIPDISSVP
                     AAQARSSAIRQEDKIKLVYRLGNFLLEASAKHSFALAVSGFDYIDEDSLSVLNYIMRS
                     EGKGKIYLLLGAGIQSIEENDKLKEFCSSLAEAGYAECIELNNLNINETAEYIRLLLG
                     MDKPFLDFAAKVYKETEGNPKFIYEIIYFLVINNHIFVNDKGEWVLNRVDLNKLNLSF
                     NIDDFILNKISKLSPLQKEIITTVSIFNTAVSPDILKKMVDMSEDKLNAQLNQLDTIN
                     ILSKKVDDWGISFDFSSINLKKSIYERIPQDIKLKSHLIASYILEDKFNKEKRQNKDE
                     LIFHMAKAMRYKEAIEYLMNSAEKMISSNLYSQAIQFLMQSYNLFAPEDVSNDKKIVC
                     SKLGELYEQIGEYSNSMYFYDEAERSAVIVNDSKVLIDIFIKKFSLSYKIGDKKKAIE
                     YAVKAKRLLKRFSYPGGLYELVICFNDVMSNKRKYSSYIKVLKKLIDTIDTERHECYY
                     ARLLVTYGRTICNKNRYEEGLSELMKGMAILEKLSEYKYMPYVLNSLGYVYSEYYNDM
                     EKAREYYEKCLSVSQKLNDLSSMEKSYNNIAEIYRIEDRYSEAIEYYNKGLQVVNVSR
                     NMMIKALLNTNLIIASIGMEDLKKAALYIDISEELLRNLKDSGNVMHFFYQCKAEFLY
                     AMGEYERAAEYAQKSVNMCITWGITENYEAHFMKLLSEIQLSGKLNHGRIKEILDKVF
                     EVNWYKLGRTACVKLAEIYISKGMRKKGLELLARGLRYASNIDTDMLRLRYDYVEALA
                     KKGAERLEGLTKLAGLMESAENNEIKWKIYKAIAEELMAQKNYREALKYLITSLSYLR
                     KLVYNVPDEYKIRFINSHDRNSVKENLCRAAELMTGNEPGEKAAKEYMTGNISLQEMD
                     KYFDYTEYRDMYRYKEADETAAGEDLDTSTRVKFLGKVQELVSRFSDDETANLKHMVD
                     LFAEITQAKNAFIATLQEDGSLNVIVSYNRYQETPFYKYLIEQAKQKRDSIIVNDTFD
                     YNAKKGDILIPKDITAVFCIPIMIQKERDGIGIELVEERRRYKEQGDNPIIGYIYLDT
                     DSIISNFTQESSLFCMMAAKIAYVLTDNYNMKVMSTVDKLTRLYTRKHFESALSKELA
                     YAEKEGGQFSIIMADIDKFKSVNDRFGHQKGDEVLQNISSIIMSSVRKGDICGRYGGE
                     EIIILLPGTDAKGAYSVAEKIRKKIENAKLLGLNTPLTISLGISSYPEHGTWAKDLID
                     KADQALYHVKESGRNGSRIYELNMSNSIKRIDRLAGIISGNIVEDQRKTETMLEILDL
                     QRDSDKTLEEKLFSFLGRIIEVSEAQTGGIFCIEEAGNEKRISRKILRKRLADKEEEV
                     YYNEGIVQKCMETGKGEYQIDWNSYPGIDILTGMPDWQSVIAVPMIDRGRIKGVTYLS
                     VPIKSKEFDAGTYNYVKTLSDIMAAAF"
     gene            complement(11278..>15498)
                     /locus_tag="PKI50_09280"
     CDS             complement(11278..>15498)
                     /locus_tag="PKI50_09280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT03932.1"
                     /translation="LSSFIEEASKKFQCIFIIENFQWIDEDSLSLIDGMLKSRNSSSA
                     YIVLATDKETYSKNIKLKEYCRKLKKANLMNNTIVLKNFNFEETAGFIKLILSLDKVP
                     HEFAKIIYDRTKGSPDYIYDTIYMLFSNNNIYIDDKGCWVLDNVNYELLNVSYAEDMD
                     MLNSIYKLDSNYQDILKAVSIFDSAVSADIAEGFVEVKGERLVSQMNYLSYISIFVRK
                     QNDWGVSYSFSSLKLKESIYKSIPKELRRKYHEKASYILKSKLSDKNIKGEDELMRQM
                     LRANWHLEVKEYISDSVRKMMENNSIYQTIQFLEHVNELLSKENVTEERISVCSILGE
                     LYERIGEYSKAVYCFNILEDIAERKGNNCLLADVYINKYSLLYKLGDRKVSLKYLAYA
                     KNLLRTIDYKKGMYEHIIVMHRMMFHKRKFSSYIRILESALKEIDKEEYKFIYARMLG
                     IYGRLKAYKGEYDEGLIMLEKSIQILESIGNYPKMLYPLNSIGTIYYNHYTDIQKARE
                     YYERCLSISQRVGNVYYESISYNSIADQYRMEDKYSTALQFYQNSLKKAKQIRDKYTE
                     FIINLNMSLTNIEIEDYKKVLLTLDDMEEEIANSRYSGDLMDYFYQCKAEFLYAMGEY
                     ERAAEYAQKSVNMCITWGITENYEAHFMKLLSEIQLSGKLNHGRIKEILDKVFEVNWY
                     KLGRTACVKLAEIYISKGMRKKGLELLARGLRYASNIDTDMLRLRYDYVEALAKKGAE
                     RLEGLTKLAGLMESAENNEIKWKIYKAIAEELMAQKNYREALKYLITSLSYLRKLVYN
                     VPDEYKIRFINSHDRNSVKENLCRAAELMTGNEPGEKAAKEYMTGNISLQEMDKYFDY
                     TEYRDMYRYKEADETAAGEDLDTSTRVKFLGKVQELVSRFSDDETANLKHMVDLFAEI
                     TQAKNAFIATLQEDGSLNVIVSYNRYQETPFYKYLIEQAKQKRDSIIVNDTFDYNAKK
                     GDILIPKDITAVFCIPIMIQKERDGIGIELVEERRRYKEQGDNPIIGYIYLDTDSIIS
                     NFTQESSLFCMMAAKIAYVLTDNYNMKVMSTVDKLTRLYTRKHFESALSKELAYAEKE
                     GGQFSIIMADIDKFKSVNDRFGHQKGDEVLQNISSIIMSSVRKGDICGRYGGEEIIIL
                     LPGTDAKGAYSVAEKIRKKIENAKLLGLNTPLTISLGISSYPEHGTWAKDLIDKADQA
                     LYHVKESGRNGSRIYELNMSNSIKRIDRLAGIISGNIVEDQRKTETMLEILDLQRDSD
                     KTLEEKLFSFLGRIIEVSEAQTGGIFCIEEAGNEKRISRKILRKRLADKEEEVYYNEG
                     IVQKCMETGKGEYQIDWNSYPGIDILTGMPDWQSVIAVPMIDRGRIKGVTYLSVPIKS
                     KEFDAGTYNYVKTLSDIMAAALRTELMI"
     assembly_gap    15499..15598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15599..16510)
                     /locus_tag="PKI50_09285"
     CDS             complement(<15599..16510)
                     /locus_tag="PKI50_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03933.1"
                     /translation="MQFINEQYKVVKEKSSDAYGTTYIVEDIQKDSLLKHLRIINLQN
                     ETRDFIDYMKNNFYNYSKYYHPYLIDFHFFNRIRLIDYKHTVLNHYYYTYDYFEGVNL
                     FDYCKGKDFDSILDLAAELCEAVKFLHLRGFLLCSVDSNDFQIVRDGEKGHLKILALP
                     YPRGTGSRIIINKKSTCFAAPELNKDESYSVLTDVYIIGSVISCMLKAFNHSEEKSEA
                     NHRAIMDIIKKCMAEKPEDRFQTIDQIIASINNYFGKEYRTINKQYIQAMPQYRINPS
                     SRHYLIDKILNNAKEHFFINRPNKVSLV"
BASE COUNT         4338 a         3942 c         2808 g         5751 t
ORIGIN      
        1 gcaaatgaag agctggaata tgaatatgat attgagttca aacagggagg atatctcata
       61 gttgcttcca cggaaaaaga agtcaaccag tttaagaaaa atatagcttt gcaaaacagc
      121 ctgggtattc cttcaagatt gctgactccc aaggaagcta aggaaatagt tcctcatatg
      181 gatgaaacaa aaatcattgc cgccacattt aacccaaaag acggacacct taaccccttc
      241 catactactc aggcttatgc caatgcagca gtcaggctgg gtgttgagat aatgaaacat
      301 acaaccgtta ccggaattaa ggttgaaaat gggaaagtgg ccggcgtgca gactgataag
      361 ggttatattt ccactccggt tgtcgtaaat gcagcaggtg ggtattcgca gcttattgca
      421 aaaatggtgg atatagatct tccgatatat tcagaaaggc atcagattct tgtaactgag
      481 cctgtagctc ctatgcaggg gccgatgttt atgtccttct cccttaatat atactgccag
      541 cagacacctc acggcagctt cataatgggc agaggcgatg caaatgagcc cagggatttg
      601 agaatcacat ccagttggca cttcatggag gaaatggcca agacctgtac tgaattgctg
      661 cctcctttaa aagaattaag agtagtacgt caatggtccg gtttgtataa tatttctccg
      721 gaccgtcaac cgatatacgg accggttaaa gaggtggaag gcttctatct tgcatgcggt
      781 tacagcggac atggctttat gttcgggcct gtaacgggag ttgttatggc tgaagccata
      841 cttggagaaa ctcctacaat tccggttcat atgctggata aggaccgatt cgaacgcgga
      901 gagctattgc ttgaaccctc tgtagtataa gaatcaatta aagcaaaaaa attccggatg
      961 gatcataagg cccatccgga attttacttt tatccttgta caatcattcc ttctctaagt
     1021 tcctccgccg ttttcttatc tctcaggatt tccaccaggt acaaggcaaa atagatagcg
     1081 gtacccggcc cctttgaagt tatgaaatta cttccatgtg agactatatt tttgcctatt
     1141 actgctcctt taagggtttc ctcaaaaccg ggatagcata cggcttcctt cttgtccaga
     1201 agccccagtt tccccagaat gctgggagct gcgcatattg ccgctatggg tttgcccttt
     1261 tcagcaaaat ccattatgat ctttctcagg ccgttatgtt cccccaagta tcttgtaccg
     1321 ggcatccctc ccggcagaac tatcatatcc tgccccatat tatcggtatt ttcgaaaatc
     1381 tcatcggcgg agacaacaat cccatgggcc cctgtaacat tcttttttcc cgttattgat
     1441 atcattcttg catctatttc cgcccgcctt aatgtatcga ccacagtaac ggcttcaatt
     1501 tcctcaaagc cttccgccaa atagacatat acttttttca ttccgtaatc ctcctttcaa
     1561 aattcttcat agccctgcca taatcgcgaa acccgtaact cgaagcacgt aaccatactc
     1621 tttaggaata cccggaaact atatctttcc ggttataacg aaatgcagct tggcaacctc
     1681 tgacagcttc tctttcccgt gaagcagtac aaattccttt gcagcctctg tcaccattga
     1741 agagcttccc ttgggaaatt ccatcccgta tttctccgcc agcttattca ttgctttcac
     1801 atattcattt cttgcaagaa tactggcagc ggccaccact acattttctt ccgctctcgg
     1861 cctttgctcc aggtttatat tctttccttt ctccagcagt gcgttcttta taagctcagg
     1921 gctgccgaac tggtcagaca gtgcgtaact gcagtctgtt tcttccagca ggttttcgat
     1981 tacccgcgca tgtccccacg caagaagctt gttcagattt tcaatctttt cgtataattc
     2041 gttatacctc ttgttgccga tgataaccac agaatacttg catattccgg ctatctcctc
     2101 agccagctta cggatcctgg catcagaaag agttttgctg tctgcggctc ctgtttccat
     2161 aagctttttc ttcattcctg catctgcata tactcccgca atcaccaaag gcccgaaata
     2221 atcgccctta cctgattcat cggtgccgat aagcccggca tcagcctctt gcggctcttc
     2281 tgccttttct gccttcaggc ttcctgttct caaaagccca tcaatcagct tataggccgc
     2341 ttcatctttt atttgcgata agtcacacct tgttccgttt ttcttatttt cgtaaactct
     2401 tataagatgc ttctttccgc tgataattat actgaactga agcccataat taatatcttc
     2461 atataaactt atttcgattc cattttcttc aagtatattc ttcaaatgat tataatactg
     2521 gtacttatcc tttgttccgt tcaattgcct cactccaaaa aatgtttata ggcgcagcct
     2581 taaagccgat ttattcactg attgccctgt atatttgtca gttcactaag ctgttttaac
     2641 agttcttcca gggcttttat cttggagccg tactccgccc aattgccgga tcgttgtgca
     2701 ttcattgcct gttcaaatgt atcatttgct tcctttatca gcttttccgc cgaagtatcg
     2761 atatttcccc ccggagttgg tacaggttca tcctgcgttt tttctcccgg tttttctttt
     2821 acggggaaca ccctgtccaa ggcttcatcc aatgtatctt ccattacaac cttattttta
     2881 aaatatacta ttactttctt ctgctccggg agtgcattgg catttaatgc ttttacataa
     2941 ataggttcta catacagtat cgattcttca atgggtattg taagcatatt tcctcttact
     3001 acctgtgagt ttcctcccgt tgcaagcaaa ttcagctgcg ggcctattat ggtgtcctga
     3061 ctgactatgc cctctatctg catagggcct tctactattt tccctgacgg gaagctgtac
     3121 agtattattt ccccataatt ctccccgtcg ttctttaccg caagccagga tatcatattg
     3181 gtcttccctt gaggggtata tggaactatc agcaaaaatt cttccttttt gctgtccgga
     3241 agcctcatta taagatatga ggactccacc aactctgcat ttgcctgatt ggtgccggtt
     3301 ccgtagattt gggtcgacac atcccacaca tcacttttat tgtagaattc ccgggcattc
     3361 ttcatatgat attttttgta tatatccgcc tgtatgtcaa acaaagtctg agggtatctt
     3421 atatgagacc ttaagccctc aggtatttca gaaatcggtt taaataacgt gttaaatatc
     3481 ttggaatatg tcttaattat ggggtcattt gcatctgcaa tatagaattc ggtagtgccg
     3541 ttgtaagcat ctattacaac cttgacggag tttcgtatat agtttgtaga tgtctggctg
     3601 ttgactctct ctgaatatgg atatctgctg cttgttgtaa atgcatctat cagccagtac
     3661 aatttcccat tataaatgac tatataagga tcttcatcat aagtgaggaa cggagcaatt
     3721 ttcttcactc tgtcaattat ctccctgttt atgagtattc tgctcttaga gttaatatcc
     3781 tgggataata ataaattgag ttctccctcc cgtaccgcaa aaagtatcct gttcaatata
     3841 ttaaggctta tacctcctgt cccgctgtat tcagtctcca tattaatatt ggtggccgga
     3901 taatcaaatt ccttctctct ggagttgaca attacaaagt cctttgtgct ctctccaaag
     3961 tatatctggg gaacatcaac ttttatgtca atatctgata tcaaaggcat gtctcttatg
     4021 aacaatctgg gctgacccga agcggtgact tcatttaccg gagacattga aactccgtat
     4081 ccgtgggtat atttaagata cttgtttatc catgacgccc ctctggcctc tgtttgcttc
     4141 ggtatatttg cagactgaag ttccctggct gatatgaata cctgcctgta tataccgttt
     4201 atcatatatc tgtcaatatc catgtcattg aattgataat agcttttcaa gccctgaatc
     4261 tgattgtaaa tatcctttgc aggcctgtaa tcatttatgg gaatattatt tactgtaacc
     4321 tcattttctt caatatcttc ccgtgtaagg gcctgattga ccgaaaactc cttctctgta
     4381 actttatcaa gattgtatgc ataatttgta taattaatat tgtactgcag gaattcttct
     4441 tctcttgcca attcatttgg tgctactatc atattctgaa ctacggcata tatcactccg
     4501 gcagcaatca tggcaactac aagaagcagc ggcccgagaa caatcagctt tatgtttttc
     4561 ttatttctgt ttaatatcaa taatgccgct gtcaatatgc atatgcccat atatatgtaa
     4621 taggcgggaa gagtaatttt gatatcagta taactggccc catatgctac tcctcttgaa
     4681 gaataaagaa ggtcaaaagt cctgagatag aaaccgaaag ctaaaaccag aaaaaagagt
     4741 cccccaagaa acataagctg ttttgaggca gtcctaagta tgtttctgta catatcctta
     4801 gaattgccga caggccttat atctatattt aacttctcgt cgggcacctt tgtggtaagg
     4861 aagttataag cattaaaaag aaaggtaatt gccgcaaatg caaatataat catcagcaga
     4921 aaattataca atttattgaa gaaaggcagt gaaaatacaa aatatcctat atcctttcca
     4981 aatataggat cagccaggcc aaagggagtc ttgttgataa atatcagaaa atcataccat
     5041 atatcaaaca cgaataacaa agaaaaaaca aaggccaagc ctaaagaacc ataaacggat
     5101 atctttgttc ttattttttt ttcaacactt gacagtacca atccgctgtg ccttacagaa
     5161 tgcgtattaa tggatttcag gtacaggtaa aacagaaaaa ataatacaaa aagggccgga
     5221 ataaaaaact gcatctgtgt aaaaagcttt ttaaagaata cctcttgata gccaagcttc
     5281 gaaaaccact ggtagttaat aaggtattcg gctatattgc taaaaaacgc aatagccagc
     5341 acaataatta ccagcaatat cgtaatgcct ttggttttaa ttctgccaaa tcctttcaat
     5401 ataacctctc cccaatttgc atatatataa attatacatt ttacattagg aatattcaaa
     5461 ctgttttagc aataaaggtc gccatgtatg gcgaccctat gtgggacgcc atacatggcg
     5521 tccatctaga atgcggcggc cataatatcg gacagtgtct tgacatagtt gtaggttccc
     5581 gcatcaaact ccttgctctt tatcggcacc gaaagatacg tcactccctt tatccttccc
     5641 ctgtctatca tcggtactgc tatcaccgat tgccagtcag gcatccccgt aagtatatct
     5701 atcccggggt aactgttcca gtctatctgg tattctccct tcccggtttc catgcatttc
     5761 tgcactatcc cctcgttgta gtaaacttcc tcttccttat ccgccagtct cttcctcaat
     5821 atcttcctgc ttatcctctt ttcattcccc gcctcttcta tacagaatat ccctcctgtc
     5881 tgtgcctccg atacttctat tatccttccc aggaagctga aaagcttttc ttccagtgtc
     5941 ttatcactgt ctctctgcag atccagtatc tcaagcattg tctctgtttt cctctgatcc
     6001 tccacaatgt ttcccgatat tatccccgca agcctgtcta ttctctttat gctgtttgac
     6061 atgttaagct catatatcct gctcccgttt ctcccggact cttttacatg gtacagcgcc
     6121 tggtctgcct tgtctatcag atcttttgcc catgtgccgt gttccggata cgaggatatc
     6181 cccaggctta tggtaagagg cgtgttaagc cccagaagct ttgcattctc tatcttcttt
     6241 cttatcttct ccgcaacgga gtaagctcct ttggcatctg tccccggaag cagtattatt
     6301 atctcttctc ctccgtatct tccgcatata tcaccttttc ttacgctgct cattattatg
     6361 ctgcttatgt tctgcagcac ttcgtctcct ttttgatggc cgaagcggtc gtttacactc
     6421 ttgaatttat ctatatccgc cattattatg gagaactgtc ccccttcctt ttccgcatac
     6481 gcaagctcct ttgacagcgc tgactcaaag tgcttcctgg tgtacagcct tgtcagcttg
     6541 tctaccgttg acattacctt catgttgtaa ttgtctgtca gtacataggc tatctttgcc
     6601 gccatcatgc agaaaaggct gctttcctgg gtgaagttgc ttattatgct gtcggtgtcc
     6661 aggtatatgt agcctattat cgggttgtcc ccttgctctt tataccttct ccgctcttcc
     6721 acaagttcta ttcctatacc gtctctttct ttttgtatca ttatcggtat gcagaacact
     6781 gctgttatat cctttggtat caatatatct ccttttttgg cattgtaatc aaaggtgtca
     6841 tttactatta tgctgtcccg tttctgcttt gcctgttcta tcaaatactt gtaaaaaggg
     6901 gtttcctgat acctgttgta ggatactatt acattcaggc tgccgtcctc ctgcagcgtc
     6961 gctatgaatg cattttttgc ctgtgttatc tctgcaaaca gatctaccat atgcttaagg
     7021 ttggcagttt catcatcact gaacctgctt accagctcct gcacctttcc gaggaatttt
     7081 acccgggtac ttgtatccag atcctcccct gccgctgttt cgtccgcttc cttgtatctg
     7141 tacatatccc tgtattctgt ataatcaaag tatttgtcca tttcctgcag gcttatattt
     7201 cccgtcatat actccttggc agctttttct cccggttcat tcccggtcat aagctccgct
     7261 gccctgcaca ggttttcttt taccgaattt ctgtcatggg agtttatgaa ccttatcttg
     7321 tattcgtcag gcacattata taccagcttc ctcaaatagc ttaaggatgt gataaggtat
     7381 ttcaatgctt ctctgtagtt cttctgcgcc atcagctctt cagctattgc cttgtatatc
     7441 ttccacttta tttcgttatt ctccgccgat tccatgagcc ctgccagctt tgtaaggcct
     7501 tccagcctct cagccccttt ctttgcaaga gcttccacat aatcgtatct cagccgcagc
     7561 atatcagtgt caatatttga tgcatacctg agtcctctgg ccagaagctc cagccctttc
     7621 ttcctcatac cctttgagat gtatatttcc gcaagcttta cacaggctgt gcgtcccagc
     7681 ttgtaccaat tcacttcaaa tactttatcc aaaatttcct tgattcttcc gtggttcagc
     7741 ttgccgctca gctgtatctc cgacagcagc ttcataaaat gtgcttcgta attctccgta
     7801 ataccccagg ttatgcacat attcacggat ttctgtgcat attccgctgc tctttcgtat
     7861 tctcccatgg cataaaggaa ttccgccttg cattgataga aaaaatgcat aacgttacct
     7921 gaatccttca ggtttctgag aagttcttcc gagatatcga tgtacagagc cgctttcttt
     7981 aaatcctcca tccctatgct tgcaattatc aggtttgtgt tgagcagtgc ctttatcatc
     8041 atattccggg aaacatttac aacctgcaat cctttattat aatattcaat agcctcgctg
     8101 tagcgatcct ctattctgta aatctccgca atgttattgt agcttttttc cattgagctt
     8161 agatcattca gcttctgact cacactaagg catttttcat aatactctct ggccttttcc
     8221 atgtcgttgt agtattcaga atacacatag cctaaagaat tcaatacata aggcatatat
     8281 ttatattcag aaagcttttc cagtatggcc atacccttca tcaattcgga aaggccctct
     8341 tcgtatctgt tcttattgca tattgttctg ccgtaagtca ccagcaatct tgcgtaatag
     8401 cattcatgcc gctcggtatc gattgtatct attaactttt tcaatacttt tatatatgaa
     8461 ctgtacttgc gtttgtttga cattacgtca ttaaaacaaa taacaagctc atagagccct
     8521 ccaggatatg aaaagcgctt cagcagcctt tttgccttta ctgcatattc aatcgctttt
     8581 ttcttgtcac ctattttgta agaaagtgaa aacttcttga taaatatgtc tatcagcact
     8641 ttcgaatcat tcactatgac cgcggatctc tctgcctcat catagaaata catggaattg
     8701 ctgtattccc ctatctgctc gtacaattca cccagcttgc tgcagacaat tttcttatca
     8761 ttgcttacat cttccggggc aaacaaatta tatgactgca taagaaattg aatagcctgg
     8821 ctgtacaagt tgctggatat catcttctcg gcagagttca taagatattc gatggcttcc
     8881 ttatacctca tagcctttgc catgtgaaaa atcagttcgt ccttgttttg ccttttttct
     8941 ttattgaatt tatcctccaa tatatatgaa gctattaaat gggattttag ctttatatct
     9001 tggggtattc tttcatatat tgatttctta aggttaatgg agctgaagtc aaaggatatt
     9061 ccccagtcat ccaccttttt tgacagtata ttgatagtat ccaactgatt tagctgtgcg
     9121 ttgagtttat cctcgctcat atccaccatt ttcttcaata tatccggaga tacggcagta
     9181 ttgaatatgg aaacagtcgt tatgatttct ttttgcaagg gacttagctt gcttatctta
     9241 ttcaggataa aatcatctat attaaaggac aggttcaatt tgttcagatc aactctgttc
     9301 agaacccatt cccctttgtc attaacaaaa atatggttgt ttataaccag gaaatatata
     9361 atctcgtata tgaactttgg attgccctct gtttctttat ataccttggc ggcaaaatca
     9421 agaaacggct tgtccatgcc gagcaaaagc cttatatatt ctgctgtctc atttatattg
     9481 agattattaa gctcaatgca ttctgcatat cctgcttctg ccagtgaact acaaaactcc
     9541 ttcagcttgt cattttcttc aatactttgt attcctgctc ccagcagaag gtatatcttg
     9601 cctttgcctt cgcttctcat tatgtaattc aagacagaga gggagtcctc atcaatataa
     9661 tcaaagccgc ttacagctaa agcaaaggaa tgcttcgcag aagcttccaa aagaaagttt
     9721 cccaatctgt ataccagctt tattttgtct tcctgcctga ttgcgctgct gcgtgcctga
     9781 gcagcgggaa cgctgcttat atccggaata atataagaaa tatccgctat atacctgtca
     9841 ataatctctt tatcagtgac cctcataatc tctttaatca gcaaaattat gtaataaaaa
     9901 ctactgacaa cattttcttt taagacattg tatactactg aaataccttc ctgctcaagc
     9961 ctataaccgg atgctttgat aaaaaccgat ttgcctgttc ccatagatcc ttttattatc
    10021 atagcctttg gttgaagttt attctcaaaa agaaaccctt ttgcagattc tattactctt
    10081 ttaaaatgat gctctcttga tacaagcctt gtaatatgcc tcggcagctt ttgaatatgc
    10141 ttcttgttta ttatattgaa attcttgttt aaccttctgt ttatatcctc aactattttt
    10201 tccaccgatt cgtacctgtc acccggcttt aaggaagtac acttttttat tatctctatt
    10261 acctgcgact tgttcttatc ctcaatattc ctgaattttt taagctcatc cttgaaattg
    10321 gaaacctcga cctttgcccc ggtaaaaata taaaacatca gtgctccaat aagataaata
    10381 tcggaagctc ttgagtaatc tccgaattga aatgcttccg gagatataaa atagttgctt
    10441 tctctgtcaa acatcacgga tttctcagta cccttaacgt atggcagctt tgaaaccttt
    10501 aaaagagcct tccctttctc atatacaaca aaaagatcat ttatgttaac actgcaaaac
    10561 agaaatcccc tcaggtgcag atattttacc agagagcaca gttgtaccga aatatccagc
    10621 agcatatcaa aattttgatt attgataaat tcaaaaagac ctaccccatt aaagtgttcg
    10681 taggtataat aatatttatt ggcaactacc ggctttgtat caatttccaa tattctgctg
    10741 aaaaaataga aatttgccag attaggatgg ctcaaattcc tgtggtcaaa catgttgagc
    10801 ttcatataat cgacaaaatc ctgagtttct ttctgataat tgattatcct caagattttg
    10861 agcatgcggc cccttctggt atcttcaaca actacagtac tcccgtagcg atcctcatta
    10921 ctgatagata taactttgta ctgggtatta ataaaccgca atttaattcc cccaagcact
    10981 aataacttgt acagttatta tatatcgtga aaaagattta ttatagcaaa atcaattgaa
    11041 tggaaatatt aatacaattt gtagtacaat ctgttaaatg aaatgtattt ataacgcatc
    11101 agcagaaaat ttttcgattg cagaaaaatt ttcttacctc tgcgcgtttc aataaggcgg
    11161 caaatatgct cacacttgtt ggaggacatt tttttgcctt attacaatat tctatcatac
    11221 tataaaaaaa aataacagca tataccaaat tttctctttg atgtacatgc tgtatggtta
    11281 tatcattaat tccgttctta atgcggcggc cataatatcg gacagtgtct tgacatagtt
    11341 gtaggttccc gcatcaaact ccttgctctt tatcggcacc gaaagatacg tcactccctt
    11401 tatccttccc ctgtctatca tcggtactgc tatcaccgat tgccagtcag gcatccccgt
    11461 aagtatatct atcccggggt aactgttcca gtctatctgg tattctccct tcccggtttc
    11521 catgcatttc tgcactatcc cctcgttgta gtaaacttcc tcttccttat ccgccagtct
    11581 cttcctcaat atcttcctgc ttatcctctt ttcattcccc gcctcttcta tacagaatat
    11641 ccctcctgtc tgtgcctccg atacttctat tatccttccc aggaagctga aaagcttttc
    11701 ttccagtgtc ttatcactgt ctctctgcag atccagtatc tcaagcattg tctctgtttt
    11761 cctctgatcc tccacaatgt ttcccgatat tatccccgca agcctgtcta ttctctttat
    11821 gctgtttgac atgttaagct catatatcct gctcccgttt ctcccggact cttttacatg
    11881 gtacagcgcc tggtctgcct tgtctatcag atcttttgcc catgtgccgt gttccggata
    11941 cgaggatatc cccaggctta tggtaagagg cgtgttaagc cccagaagct ttgcattctc
    12001 tatcttcttt cttatcttct ccgcaacgga gtaagctcct ttggcatctg tccccggaag
    12061 cagtattatt atctcttctc ctccgtatct tccgcatata tcaccttttc ttacgctgct
    12121 cattattatg ctgcttatgt tctgcagcac ttcgtctcct ttttgatggc cgaagcggtc
    12181 gtttacactc ttgaatttat ctatatccgc cattattatg gagaactgtc ccccttcctt
    12241 ttccgcatac gcaagctcct ttgacagcgc tgactcaaag tgcttcctgg tgtacagcct
    12301 tgtcagcttg tctaccgttg acattacctt catgttgtaa ttgtctgtca gtacataggc
    12361 tatctttgcc gccatcatgc agaaaaggct gctttcctgg gtgaagttgc ttattatgct
    12421 gtcggtgtcc aggtatatgt agcctattat cgggttgtcc ccttgctctt tataccttct
    12481 ccgctcttcc acaagttcta ttcctatacc gtctctttct ttttgtatca ttatcggtat
    12541 gcagaacact gctgttatat cctttggtat caatatatct ccttttttgg cattgtaatc
    12601 aaaggtgtca tttactatta tgctgtcccg tttctgcttt gcctgttcta tcaaatactt
    12661 gtaaaaaggg gtttcctgat acctgttgta ggatactatt acattcaggc tgccgtcctc
    12721 ctgcagcgtc gctatgaatg cattttttgc ctgtgttatc tctgcaaaca gatctaccat
    12781 atgcttaagg ttggcagttt catcatcact gaacctgctt accagctcct gcacctttcc
    12841 gaggaatttt acccgggtac ttgtatccag atcctcccct gccgctgttt cgtccgcttc
    12901 cttgtatctg tacatatccc tgtattctgt ataatcaaag tatttgtcca tttcctgcag
    12961 gcttatattt cccgtcatat actccttggc agctttttct cccggttcat tcccggtcat
    13021 aagctccgct gccctgcaca ggttttcttt taccgaattt ctgtcatggg agtttatgaa
    13081 ccttatcttg tattcgtcag gcacattata taccagcttc ctcaaatagc ttaaggatgt
    13141 gataaggtat ttcaatgctt ctctgtagtt cttctgcgcc atcagctctt cagctattgc
    13201 cttgtatatc ttccacttta tttcgttatt ctccgccgat tccatgagcc ctgccagctt
    13261 tgtaaggcct tccagcctct cagccccttt ctttgcaaga gcttccacat aatcgtatct
    13321 cagccgcagc atatcagtgt caatatttga tgcatacctg agtcctctgg ccagaagctc
    13381 cagccctttc ttcctcatac cctttgagat gtatatttcc gcaagcttta cacaggctgt
    13441 gcgtcccagc ttgtaccaat tcacttcaaa tactttatcc aaaatttcct tgattcttcc
    13501 gtggttcagc ttgccgctca gctgtatctc cgacagcagc ttcataaaat gtgcttcgta
    13561 attctccgta ataccccagg ttatgcacat attcacggat ttctgtgcat attccgctgc
    13621 tctttcgtat tctcccatgg cataaaggaa ttccgccttg cattgataga aatagtccat
    13681 aaggtctccc gaatatctgg agtttgctat ctcttcttcc atatcgtcaa gggtaagaag
    13741 aacctttttg tagtcctcta tttcaatgtt tgtcagggac atattaagat ttattatgaa
    13801 ttccgtatac ttatccctta tctgcttagc tttcttaagg gaattctgat agaattgcag
    13861 tgctgtggaa tacttgtctt ccattctata ctgatcggcg atactattat agcttatact
    13921 ctcataatac acattcccta ctctctgact tatgcttaaa catctttcat aatactccct
    13981 ggccttttgt atatcggtgt aatggttata atatattgtt ccaattgaat tcaacggata
    14041 caacatcttt ggataattac cgatgctttc aagaatctga atactttttt ccaacatgat
    14101 aaggccttcg tcatattcgc ccttataagc cttgagcctg ccataaatgc caagcatcct
    14161 tgcatatatg aacttgtatt cctctttatc gatttctttt aaggcacttt ccaatatcct
    14221 tatgtaactg ctgaatttcc ttttatgaaa catcattctg tgcattacaa ttatatgttc
    14281 gtacatgccc tttttataat caatggttct caaaaggttc ttggcataag caagatactt
    14341 taatgaaacc ttcctatcac ccaatttgta cagtagtgag tatttattta tatatacgtc
    14401 tgcaagcaag caattatttc ctttcctctc ggctatatcc tccaaaatgt taaagcaata
    14461 aacagctttt gaatactcac ctatacgttc atataactct cccaatatac tgcaaaccga
    14521 tattctctct tctgttacat tctccttgga cagcaattca ttaacatgct cgaggaattg
    14581 tattgtctga tatattgagt tgttttccat catcttcctg acagagtctg aaatatattc
    14641 ctttacttca agatgccaat tggctcttag catttggcgc atcagctcat cttcaccttt
    14701 tatgtttttg tcgctgagct tgcttttcag tatataagaa gctttttcat gatattttcg
    14761 tctcaattcc tttggtatgc ttttatatat cgattctttc aattttaatg aactgaagct
    14821 gtaggatacg ccccagtcat tctgttttct tacaaaaatg cttatataag aaaggtaatt
    14881 catctggctg actaatcttt cacctttaac ttcgacaaaa ccttctgcaa tatctgctga
    14941 aaccgcacta tcaaaaatgg ataccgcctt aagaatatcc tgataattgg agtcaagctt
    15001 gtaaatactg ttcagcatat ccatatcctc ggcataggaa acattcagca attcatagtt
    15061 tacattatca agcacccagc agcctttgtc gtcaatatat atgttgttgt ttgaaaaaag
    15121 catatatata gtatcatata tataatccgg acttcctttt gtcctgtcat aaataatttt
    15181 tgcgaattca tggggcactt tgtccaagct cagaataagc tttataaatc cggctgtctc
    15241 ctcaaagttg aagttcttaa gcacaatagt attattcatt aagttcgctt tcttaagctt
    15301 cctgcagtat tcctttaatt tgatgttttt ggaataggtc tccttgtctg tggcaagtac
    15361 aatataagcg cttgaactat ttcggctctt taacattcca tcaatcagac tcaatgaatc
    15421 ctcatctatc cactgaaagt tttcaataat aaaaatgcat tgaaacttct ttgaagcttc
    15481 ctctataaaa ctgcttagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
    15601 ccaaagaaac cttattgggc cgattgataa aaaaatgctc tttcgcattg tttaatatct
    15661 tgtcaataag atagtgcctt gacgatggat ttattctgta ttggggcatt gcctgaatgt
    15721 actgtttatt tattgtccta tactcttttc cgaagtaatt gtttatactt gcaataattt
    15781 gatctatggt ttgaaacctg tcttcaggtt tctcagccat gcattttttt attatatcca
    15841 taatagccct gtggttagcc tcagattttt cctcagaatg gttgaatgct ttgagcatac
    15901 aagaaatgac cgagccaata atataaacat ctgtcagaac tgaatagctc tcatccttat
    15961 ttaactccgg agctgcaaaa catgtacttt ttttgtttat tattatcctg cttccggttc
    16021 cccgaggata tggcaatgcc aatatcttca agtggccttt ttctccgtcg cggactattt
    16081 gaaaatcatt actgtcaaca ctgcaaagca agaagcctct caagtgcaag aatttaacag
    16141 cctcacacag ttctgccgcc aggtccagaa tcgaatcgaa gtctttccct ttacagtaat
    16201 caaataaatt aaccccttca aagtaatcat aggtataata atagtggttt aaaacagtat
    16261 gtttataatc aataagtctg attctgttga aaaaatgaaa atctatcaga tatggatgat
    16321 aatatttgga ataattataa aaattgtttt tcatatagtc tatgaagtcc ctggtctcat
    16381 tttggaggtt tataattctc aaatgcttaa gaaggctatc cttttgaata tcttctacta
    16441 tataggttgt accgtatgca tcggaactct tttccttgac aaccttatac tgctcgttaa
    16501 tgaactgcat ctgaaatcct cctttcttca aatcaattcg aaaaattata acgaggcaag
    16561 aagatgtcct tcacttttac aatggtaagt gctgctttct ttgactaaaa ggcggtaagc
    16621 atgtcttccg cctcgtttta accctcggat agatttttgc aggtatgcaa aaacctcatt
    16681 gaaaatttgt tataaaaatg caactgctgt ttcttaattt taccataaat ttagagaaat
    16741 ttggagaaaa tttagcaact tgcatataaa tgcaataaac gaaccgccgt ttcattgcca
    16801 acaggtaaca ggaagaatac atttcccata caaggcaagt gtataaaaaa gaacgatgga
    16861 atttcttcta tcgttcttag ttacctggcg gcgatctact ctcccgggac cctgcggtcc
    16921 gagtaccatc gacaccgga
//
LOCUS       DAOQUR010000046        16556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3973_length_16556_cov_10.285134, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000046 DAOQUR010000000
VERSION     DAOQUR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16556
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3973_length_16556_cov_10.285134"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /locus_tag="PKI50_09290"
     CDS             complement(<1..2508)
                     /locus_tag="PKI50_09290"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNT03934.1"
                     /translation="MNPQKYKTEKLVPINLPEICAPRTQLMDVYRKASEKRCIYVHAP
                     AGCGKTVSTLLWLKESEYKTIWIGLDEYDNTLAGFYRTFCMALFSIVPYEENLAELIK
                     APEFSSSPVEYAIEILSLLSFEDCTYALVFDDFHLITNKEIIKSLIYVFKRLPLSVTI
                     MVLSRNELPETFSQYEESGKIACIGADEQAFKGDEIRRHFASYGRFLTEREAEEVYAL
                     TEGWAIAVNALAMSGDFYPGEKSPSNPLRKYIKAQIWDKLDRDLRLFMLQTAVVDRFS
                     EELCRKITDNPESQQILLYLLGENMFLSRRETEYRYHNLFLDFLREELLEELQINRQA
                     LYEKAANYYYDSGDFFNALCFYIKCNCSKGIADALYHFMKFNVQSSSEMSKVYFINKL
                     PAEILEQNPCLYVSCAWCALLFGDAAELHFYSDKLYDRIYDIVRENKALLEISLFVYS
                     ADQRYTFTEQLAKLQAAAVPLDADPANIPKIISHNMPYFHRTYRDYSHYALNTEEHFA
                     EFSLVFSSLLGSEYAVIESGIRAGLLYDQNRIQDALSLVVENPASVSPELEFLSRMQI
                     AFCLYAMGKAEKTAQCRKEIEAFLKTKKLLYLLPVFSAYETKLKLLDGDSSAAKGWLE
                     NYFITESYNPEMHKIFLHFTTVRAYIVLGEYEKAELLCEKLWKLCTDYHRLLDAAEAA
                     VLLTVILRLTDREAKASALLRKTLLDMEPYGYIRVFADEGNAILPVLKKLIKKADTEN
                     GTVMPGSAYMKAVYMSVYEQSKRYKGIITNMGRKQVKLSAQQKHMIELLAKGYKNAEV
                     VELTGLSINTVRYHTKLAYQKLDVSNAKDAVLRAKELS"
     gene            2719..3060
                     /locus_tag="PKI50_09295"
     CDS             2719..3060
                     /locus_tag="PKI50_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNT03935.1"
                     /translation="MERLLDKSFDFSVRITELIKYLERENKSFPLSGRLLECAAGIGV
                     CMKQAASAGSMSFETHQKTMSYASEAEYLLELMEKTEYMTEKQSRPILGDCRGLMSAI
                     AALNIKQKGRD"
     gene            3062..4411
                     /locus_tag="PKI50_09300"
     CDS             3062..4411
                     /locus_tag="PKI50_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03936.1"
                     /translation="MSKASLIVVSILIIVCSVLFLSSCSARGPETPDVNPAAPAQAPA
                     VEAANQNTAESEAVNQDTAAAETEQQNTAEAEAEQQSTAEAEAVPDSGLTLEKLKAKV
                     QEHGYEVTPVEGYQLTDSIKPEDGFNLHYVDDNTDGFVPVFEFGSSNDALKYAEGVNS
                     AGYNLCIVNGRFVTMTGAQYGIVTYDPQADFLAGLLESEVMAAPKISGSPVIPPASDY
                     AGACKQIDSVRNVLNTLVNRTVLTHDKSAAEDKNFSAAFISFNILSSGNLGFTATLSE
                     DEASRDAMAQMWTAFGVTDMKIERTAPHTYELTGTRAGMEAPFLIRCMFEPSSGALRL
                     TEMDGDRAAELFEYVPLGGDTYAFQTINERAVVKYQGGQITGFTYSLLKSGTAAYSPD
                     ADGIYNKTGLDAAWVTSAGADLFEQLVTYDGTILKIYAESFFTGSRITADINVPQNP"
     gene            4473..6005
                     /locus_tag="PKI50_09305"
     CDS             4473..6005
                     /locus_tag="PKI50_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT03937.1"
                     /translation="MKKKLLTLLLILVMASGFTSLSVLAASPPTTISAPENFAAADYM
                     GSSVYCTLSAPEDLRRLIVQTDEERGYYMSIMAQVDFKTDGGSWHYSPEWDNRDTYSK
                     YVLYFYNSLTGGDGGIFLGGQYLVFRNMFPEESNIPVESGFNSWDWYKSHSMTLRARF
                     AIIFGDNVVFSDWSEEYTLSNSSKMDYKKIMNENAPTLVSSKIEARGPDNVPWVVLLL
                     AQHPAAAQKFNAASFDNMWTEIWLRKLGDTDFIKVGAERFHNERISLDIGAYFQENLA
                     SYDAQAYEVKVRYTIDERSYPQSGVTSETWLHSPFSNTLSYGMPVWSAASNWAADELK
                     KADESGLIPDILKNTDLTKPITREEFCELALVLYEKTTGKTPAPYAPNPFIDTTNPQI
                     LKAFALGITKGTSATTFEPNKTITRQECATMLFRTIKGIVPEADYSITGVPDFPDQKN
                     IDSWAVEGTKYMSKLGIIKGDDKGYFMPKATTTAQQATGYGTATREAAILMSVRTYGV
                     MP"
     gene            6002..6697
                     /locus_tag="PKI50_09310"
     CDS             6002..6697
                     /locus_tag="PKI50_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03938.1"
                     /translation="MMRHTFRYLVIIAAAGLLFLCACSPQDQTGENTSHASSASAQQE
                     ETNTSSKEETAATSEESAAEVECNWTLEVSDTVTTQLNGYDFKCTLAVMAVKLGGKDE
                     LGTYRGIVTLKYQYDMKQGNVAGNAAGEGQEIDAVIEVVQYEEQAYNEATGQTVLAEL
                     VQYDAMAIGSLVLTGSGEAAESAGGASWSTSDRKTIPVPYRMAVDGGKVTIELYTIAP
                     GVKFNGMITGTPI"
     gene            complement(6767..7525)
                     /locus_tag="PKI50_09315"
     CDS             complement(6767..7525)
                     /locus_tag="PKI50_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNT03939.1"
                     /translation="MKFGKSLVFILAVILLTTSVTFADVLKNGCRGEEVSRIQSQLKE
                     KGYFTEEVTGYFGNVTEEAVRKFQEDNNLTVDGIVGPDTGKVLFGTEWTYTAEVEALD
                     WWSEASKIFERGTVAKVTDVRTGETFEIVRTYGGNHADCEARTAEDSKKIKEIWGGWS
                     WERRPIVVEVDGRKIAASMAAMPHAGVDKSPADKYISSRSGGYGRGANLDKIKNNGMS
                     GVVDVHFLNSRTHNTNKVDNKHQACVKEAAASGN"
     gene            complement(7664..8047)
                     /locus_tag="PKI50_09320"
     CDS             complement(7664..8047)
                     /locus_tag="PKI50_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03940.1"
                     /translation="MDTEIVLPQEGRERAGTAEAKLAGRMLLTSSIIVNVIEEDTFLV
                     LASVLSVIASLLTIKAARLEAEEQQIAPGVTTFANSLKLIGSHTGLFVSIILLWALLI
                     EISIKQTTELSAQPEMAGTLGAFMV"
     gene            complement(8726..10891)
                     /locus_tag="PKI50_09325"
                     /pseudo
     CDS             complement(8726..10891)
                     /locus_tag="PKI50_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017810617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(10924..11292)
                     /locus_tag="PKI50_09330"
     CDS             complement(10924..11292)
                     /locus_tag="PKI50_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT03941.1"
                     /translation="MEENRVSIESCSCSMIHEDAVSKVRNSMPEEETLYDLAELFKVF
                     GDTTRIKILCALFESEMCVCDIAALLSMNQSAISHQLRVLKQARLVKYRKEGKVVYYS
                     LDDEHVKQIFDQGLVHIRER"
     gene            complement(11882..12631)
                     /locus_tag="PKI50_09335"
     CDS             complement(11882..12631)
                     /locus_tag="PKI50_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03942.1"
                     /translation="MGETISEKTEGRVSYHDSGEEMLPRIREDGMSNAFDRYAQQEKI
                     RCKFCLQGLSCQLCTNGPCRISEKSGQDKGVCGIGGDTMAMRNMLLRNIMGAGTYSHH
                     AYEAFRTLRETAQGKTPFKITDANKLKWMCDTVGIDSKQDVNKMAGEMADHLDVDIPQ
                     LPIAVTAPEWMEQKATIDGFFAAAYGAYTRLSPIPFVTGAPGLVKLLTEDVEGLTGGK
                     IALGDDPAEAANNIEAHIISKRKGLGLREAD"
     gene            complement(12759..13214)
                     /locus_tag="PKI50_09340"
     CDS             complement(12759..13214)
                     /locus_tag="PKI50_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT03943.1"
                     /translation="MKRIVILLLAVLLGAAVFSACSTKPQQDISQSPAEIVQPEQSPA
                     EVVQPESISPEEAYKRLESEEDIILLDVRTQGEYEEQHIPGSILIPVNDLEKRAEAEL
                     TDKDADIIVYCASGKRSSSAANILAGLGYSKVYNMGGIMDWPYELESGE"
     gene            complement(13231..13809)
                     /locus_tag="PKI50_09345"
     CDS             complement(13231..13809)
                     /locus_tag="PKI50_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNT03944.1"
                     /translation="MVNTIPSRAAALELLQEYNKNDSLVKHALTVESVMRHFAGLLGE
                     DEEKWGIIGLLHDIDYEMYPEQHCIKAKEILTERGWPEDYIHAVLSHGWKLCSDVEPT
                     EKMEKILYTIDELTGLIAATAIMRPSRSILDMEVKSVKKKWKQKSFAAGVNREVIEEG
                     AALLGMEMDTIIGETINGMKKAAEEIGLKGSL"
     gene            complement(13846..14793)
                     /locus_tag="PKI50_09350"
     CDS             complement(13846..14793)
                     /locus_tag="PKI50_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNT03945.1"
                     /translation="MADKIATREAYGNALAEFGSDNRIVVFDADLSKSTKTDNFRKKY
                     PERFFNMGIAEGNMMATAAGMASCGKVVFASTFAMFAAGRAFEQIRNSICYPKLNVKI
                     GATHAGLSVGEDGASHQAIEDIAIMRALPNMTVISPADAVETKYAVKAAIEHEGPVYL
                     RLGRLNVPVIYNESSYKFEIGKGIVLAEGNDAAIIATGLMVSAALEAKELLQQEGINA
                     RVIDIHTIKPIDADIIVKAARETGAIVTAEEHNIIGGLGSAVAEVLSENYPVPLVRVG
                     VQDKFGKSGKPDILLQMYGLTAADIAKSTKAVLKKKAGR"
     gene            complement(14795..15640)
                     /locus_tag="PKI50_09355"
     CDS             complement(14795..15640)
                     /locus_tag="PKI50_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT03946.1"
                     /translation="MKKPEIKDLKKYAVKVRKHIIDEVYSAASGHPGGSLSCTEILTV
                     LYFAEMRVNTKQPLWEDRDRFVLSKGHCSPALYAVLAEKGFFPKEDLLTFRKADSYLE
                     GHPSMRYVPGVDMSTGSLGQGISAAVGMAMAGKLDKKDCRVYAVLGDGELQEGEVWEA
                     FMAAAHYKLDNLTAFLDHNGLQIDGRITDVMSPEPVADKFKAFGWNVLKTDGHDIEKI
                     LESIGQAKDCKGKPTIIIAETVKGKGVSYMENQAGWHGSAPNKEQRDQAMAELDKVLA
                     ELEVE"
     gene            complement(15667..16086)
                     /locus_tag="PKI50_09360"
     CDS             complement(15667..16086)
                     /locus_tag="PKI50_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNT03947.1"
                     /translation="MKHTKIGSKYFVRIDKGEELVASLYKFCTDNGLKLGSVIGIGAA
                     NRITIGVFNTVTKEYHNQELTGEYEITNLTGSITEKDGEVYLHLHITLGDEEYNAHGG
                     HLNQCWISGTCELIIDAVDGEIGRVFDDYCGLNIWKL"
     gene            16276..16470
                     /locus_tag="PKI50_09365"
     CDS             16276..16470
                     /locus_tag="PKI50_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03948.1"
                     /translation="MIKIDNDTVRFEGYELENLVVLLEQVVRNSIIDSDSREFAISFL
                     KAFSDCFGEDVIKKFNSIRK"
BASE COUNT         4318 a         4196 c         3354 g         4688 t
ORIGIN      
        1 actgagttct tttgcccgga gcactgcatc cttcgcattg cttacatcca gcttttgata
       61 ggccaatttg gtatggtatc ggacagtatt aatagaaagg ccggtcagtt caacgacttc
      121 agcattttta tagcctttag ccagtaattc gatcatatgt ttttgctgtg ctgaaagctt
      181 tacctgtttt cggcccatgt tggtaattat ccctttgtaa cgctttgact gctcataaac
      241 cgacatgtac acagctttca tataagcgct gccgggcata accgttccat tttccgtatc
      301 agcctttttt atcagttttt ttagtaccgg taaaatcgca ttaccctcat ccgcaaatac
      361 tctgatataa ccgtagggct ccatatccaa cagggttttt cgaagaaggg cggaagcttt
      421 tgcttctctg tccgtcaacc gcagaataac ggtcagcagc acggcggctt cagccgcatc
      481 aagaagtctg tgataatccg tacacagctt ccaaagcttt tcgcaaagta attccgcttt
      541 ttcatattct ccaagaacga tataagccct tacagttgta aaatgcagaa aaatcttatg
      601 catctcagga ttataggatt cggtaatgaa ataattttca agccatccct ttgccgcgct
      661 gctgtcgccg tccaggagct tcagctttgt ttcataagct gagaatacag gaagcaggta
      721 caacagtttt ttcgttttta aaaaagcttc aatttctttc ctgcattggg cggtcttctc
      781 cgctttgccc attgcgtata ggcagaaggc aatctgcatt ctggacagaa attcgagttc
      841 aggtgaaacg gaagccggat tttcaaccac aagtgaaagc gcatcctgaa ttctgttttg
      901 gtcatacagc agtccggcac gtattcccga ttcaataact gcatactcac tgcccaaaag
      961 agatgaaaac acaagagaga attcggcgaa atgctcctca gtattcagtg cataatggga
     1021 atagtccctg taagttctgt ggaaataagg catattatgg ctgattattt tcggtatatt
     1081 cgcagggtct gcatcaaggg ggacagcagc tgcctgcaat tttgcaagct gttccgtaaa
     1141 cgtatatctt tgatctgccg aatacacaaa aagagagatt tccagcagag ctttgttctc
     1201 cctgacaata tcataaatcc tgtcatacag cttgtccgaa taaaaatgca gttctgcagc
     1261 atcgccgaac aaaagagcac accaagcaca gctgacatat aagcacggat tttgctccag
     1321 gatttctgcc ggcagcttgt taatgaaata cactttggac atctccgagc ttgattgaac
     1381 gttgaacttc ataaagtgat acaaggcatc ggcaatcccc ttgctgcaat tacactttat
     1441 ataaaaacaa agtgcgttga aaaaatcccc tgaatcataa taataattag ccgccttttc
     1501 atacagagcc tgccgattta tttggagctc ttccaataac tcctcccgca agaaatccag
     1561 aaacaaatta tggtatctgt attctgtttc ccgacggctg agaaacatat tttcgccgag
     1621 caaatagaga agaatttgct ggctttccgg gttgtcagta atcttccggc aaagctcttc
     1681 agaaaatcta tccacaacgg cggtctgcag cataaacagg cgcaaatccc tgtcaagctt
     1741 atcccatatt tgtgccttta tatatttgcg cagaggattg cttggagatt tttcccccgg
     1801 ataaaaatct ccgctcattg ccagagcgtt taccgcgata gcccagcctt cggttaaagc
     1861 gtacacttct tcagcctccc tctcggtaag gaaacggcca tagcttgcga aatgccgccg
     1921 tatttcatct cctttaaatg cctgctcatc agcaccgata caagcaattt ttccgctttc
     1981 ttcgtattgg gaaaaggtct ccggcagctc gttgcggctt aataccatga tagtaacgga
     2041 taacggaagc cgtttaaaaa cataaatcag agacttgata atttctttat tggtaatgag
     2101 atggaagtca tcaaacacaa gggcgtatgt acaatcctca aaagaaagca acgagaggat
     2161 ctcgatggcg tattcaaccg gtgatgaact aaattcaggg gctttgatca gttccgccag
     2221 attttcctca taggggacta tagaaaagag cgccatacag aaagttcgat aaaagcctgc
     2281 cagcgtgtta tcatattcgt caagaccaat ccagatggtt ttgtattccg actccttcag
     2341 ccacaaaagg gtagatacgg tttttccgca gccggcaggc gcatgtacgt aaatacatct
     2401 tttttcgctg gctttcctgt aaacatccat caattgagta cgcggagcac atatctcagg
     2461 aaggtttatc ggaacaagct tttcggtttt atatttctga gggttcataa aacacctcct
     2521 ttttttgtta ttattatact tagtatatca aaatttacaa aatattcaat cgagttaggc
     2581 atatattccc acattattgt tttgagtctc ccaaaataat tttcaattat cccacatcaa
     2641 tgcggtttta taatcataaa taccatatta aaatgaaata aagataccgg accctattac
     2701 ggaaaggaga tgtcagaagt ggagcggctt cttgacaaaa gctttgattt ttctgttcgc
     2761 atcacagagc tgattaaata ccttgagaga gagaataaga gctttccttt gagcgggagg
     2821 ctgcttgaat gtgcagccgg aataggcgta tgcatgaaac aggccgcatc tgccggcagt
     2881 atgtcctttg aaacccatca gaaaaccatg agctatgctt cagaagcgga atatctgctg
     2941 gaactgatgg aaaaaacaga atacatgact gaaaagcaaa gcaggcctat ccttggcgat
     3001 tgccgggggc ttatgtcggc tattgcagct ctgaacatca aacagaaagg aagggattaa
     3061 catgagtaag gcgagtttaa tagttgtctc aatcttaatc atagtttgtt ccgtactgtt
     3121 cttatcctca tgctccgcaa ggggccctga gactccggat gtcaatcccg cagcgccggc
     3181 acaagcgcct gcggtggaag ccgcaaatca aaatacggca gagtcagagg ctgtaaatca
     3241 ggatacagca gcagcagaaa ctgagcaaca aaatacagca gaggcagaag ctgagcagca
     3301 aagcacggca gaggcagaag ctgtacccga cagcggcctg acactggaga aacttaaggc
     3361 gaaggtgcaa gagcatggat acgaagtaac acccgttgag ggttatcagc ttacggacag
     3421 cataaagcct gaagacggct tcaacctgca ctatgtagac gataacaccg acgggtttgt
     3481 cccggtgttt gaattcggca gctccaatga tgcattgaaa tatgccgagg gcgtaaactc
     3541 ggcgggatat aatctttgca tcgtcaacgg ccgctttgtc acaatgacgg gggcacagta
     3601 cggcatcgtg acatacgacc ctcaggcgga cttccttgcc ggtctgctcg aaagcgaggt
     3661 tatggcagcc ccgaaaattt ccggcagtcc tgttatacct ccggccagtg attatgccgg
     3721 cgcctgcaaa cagattgaca gtgtcagaaa tgtgcttaat acactcgtca acagaactgt
     3781 attaactcac gataagagcg ccgcggaaga taagaacttt tcagctgcgt ttatcagctt
     3841 taacatactt tcttcgggta atcttgggtt tacggctact ctgagcgagg acgaagccag
     3901 ccgcgatgct atggctcaga tgtggaccgc cttcggcgta acggatatga agattgaaag
     3961 aaccgccccg catacttacg aactgacggg aacccgggca ggaatggaag caccgtttct
     4021 gatccgctgc atgttcgaac cgtcctccgg agccctgcgg cttacagaga tggacggtga
     4081 ccgggcagct gagctgtttg aatatgttcc gttgggcggg gatacctatg cctttcaaac
     4141 aattaacgag cgtgccgtcg taaagtatca ggggggccag atcaccggct tcacttattc
     4201 gcttttaaaa tccgggactg ctgcatacag ccccgacgca gacggtattt ataacaaaac
     4261 cggacttgac gcggcgtggg taacgtcagc cggagcggac ctcttcgagc agcttgtcac
     4321 ctacgacgga acgattctga aaatctacgc cgaaagtttc tttacaggca gccgcattac
     4381 agcggatata aatgtgcctc aaaatcctta atgcaccaat taccgaaatt tcgtgaaatt
     4441 ttataaaaat aaatatttaa ggaggcgtcc aaatgaaaaa gaagttactg acattattat
     4501 tgatacttgt aatggcttcg ggttttacct ccctttcggt gctggcagca tccccgccca
     4561 caacgatttc cgcgcccgaa aactttgccg ctgccgatta catgggcagt tccgtctact
     4621 gcaccctgag cgcgccggag gacttgcgca ggctcattgt ccagacggat gaggaacggg
     4681 gctattacat gtcgattatg gcccaggtgg atttcaaaac agatggagga agctggcatt
     4741 attcaccgga gtgggacaac agggacacct attcgaaata cgtgctgtat ttttacaaca
     4801 gtctgaccgg cggtgacggc ggaatatttc tgggcggcca gtatctcgtt ttcagaaata
     4861 tgttcccgga ggaatcaaat attcctgtcg agtcaggctt taacagctgg gactggtaca
     4921 aaagccactc catgacactg cgggcacgct ttgccatcat attcggagat aacgttgtgt
     4981 tttccgactg gtcggaggag tataccctgt caaacagcag taaaatggac tataaaaaaa
     5041 ttatgaatga aaacgcaccc accctcgtgt cctccaaaat tgaagcgcgg ggcccggaca
     5101 acgtcccttg ggttgtttta ctgttagcgc agcatcccgc tgcggcgcag aagttcaatg
     5161 cggcctcatt cgacaatatg tggactgaaa tctggcttcg caagcttggc gataccgatt
     5221 tcataaaagt cggcgctgaa cggttccata atgaaagaat cagccttgac ataggcgctt
     5281 attttcagga aaaccttgcc agctacgatg cacaggccta cgaggtcaag gtgcgctaca
     5341 caatagacga acggtcttac cctcaatcag gcgtaacatc ggaaacttgg ctgcacagcc
     5401 ccttttccaa tacgctttct tatggaatgc ccgtgtggag cgcggcttcc aactgggctg
     5461 cggatgaact gaaaaaggct gatgagtccg gcctcattcc cgatattctt aaaaatacgg
     5521 atttgacaaa gcccattacg cgggaagaat tctgcgaact ggcgctggta ctgtacgaaa
     5581 agactacggg caaaactccc gcgccttacg cccccaaccc ctttatcgat acgacaaatc
     5641 cgcagattct gaaagctttt gccttaggta tcacaaaagg tacatcagca accacctttg
     5701 aacccaataa aaccataacc aggcaggagt gcgccacaat gctgttcagg acaatcaaag
     5761 gcatcgtgcc ggaagccgac tattccatta ccggtgtgcc tgatttcccg gatcagaaga
     5821 atatcgactc atgggcggtt gaaggcacga aatatatgtc aaagctgggt atcatcaaag
     5881 gcgatgataa aggctacttc atgcctaaag caacgacaac cgcgcagcag gcaaccggct
     5941 atggcacggc gacaagggag gcagcgatcc tcatgagtgt gaggacttac ggtgtaatgc
     6001 catgatgagg cataccttcc ggtatttggt catcattgcc gccgcaggat tacttttcct
     6061 atgcgcatgc agtcctcagg atcagaccgg tgaaaacacc tcccatgcgt cttcagcctc
     6121 ggctcagcag gaggaaacaa atacctcttc aaaggaagag acggcagcaa cctccgaaga
     6181 atccgctgcg gaagttgagt gcaactggac ccttgaagtc agcgatacag taacgacaca
     6241 gcttaacggc tacgacttta aatgcaccct tgcggttatg gcagtcaagc tgggcggtaa
     6301 ggatgaactg ggaacctaca ggggaattgt cactcttaaa tatcaatatg acatgaagca
     6361 gggcaatgtt gccggcaacg ccgcaggtga gggtcaggaa atagatgccg tcattgaggt
     6421 cgtacaatac gaggaacagg cgtacaatga ggcaacaggg cagacagttc tggcagaact
     6481 tgttcaatat gacgcaatgg ccataggcag cctggttttg accggaagcg gtgaagccgc
     6541 cgaatctgcc ggaggcgcca gctggagcac aagtgacagg aagacaatcc cggtgccgta
     6601 taggatggca gtggacggcg gcaaggtaac cattgagctt tatactattg ctccgggagt
     6661 caaattcaac ggaatgatta caggaactcc aatttgagga agtaccgatg ataataagaa
     6721 agccagccac caaatcgtga ctggctttct gaatttattt atcatattaa ttaccgcttg
     6781 cagctgcttc ttttacacag gcttgatgct tattgtccac tttgtttgta ttgtgggtcc
     6841 tgctgttaag gaaatgaaca tctactacac cgctcatccc attgttcttt atcttgtcaa
     6901 gatttgcacc tctgccgtaa ccgccgctcc tgctgctgat atacttatct gcgggggact
     6961 tgtccactcc tgcatgaggc atagccgcca ttgatgccgc tatcttccgc ccgtcaactt
     7021 ccacaactat cggccttcgt tcccagctcc agccgcccca aatctctttg atctttttgg
     7081 aatcctccgc ggtcctggcc tcacagtcag cgtggttgcc gccgtatgta cggacaattt
     7141 caaaggtttc tcccgttctt acgtcggtta cctttgccac cgttcctctt tcaaaaattt
     7201 tggatgcttc agaccaccaa tccagagcct ctacttcagc agtatatgtc cattcagttc
     7261 caaaaagcac tttccctgta tccgggccta caatcccatc aacagtcaga ttgttatcct
     7321 cctggaactt tctcaccgct tcttcagtaa cgtttccgaa gtaacctgtt acttcctcgg
     7381 taaaataccc tttctccttc agctgagact gaatcctgga tacttcctcg cccctgcacc
     7441 cgttttttag aacatcggca aaagtaacag aagtagttaa aagtatcact gcaagtataa
     7501 ataccaatga tttgccaaac ttcaacactg tctccccttt ctgcttacgg acttcactta
     7561 acccgttttg tccctcgctc ttctgaggaa ttcagccaat tatatagctt gtctacattt
     7621 tagcaatttt ttattaccaa ttcaatggta aaaaaataca gtgttatacc ataaatgcac
     7681 ctaaagtccc tgccatttca ggttgggctg ataattcagt cgtttgtttt attgatattt
     7741 caataagcaa agcccaaagg agaataatcg atacaaataa tcccgtatga ctgcctataa
     7801 gcttcaggct attcgcaaag gtagtcaccc ccggggctat ctgctgctct tcggcttcca
     7861 gcctggcagc ctttattgta agcagactgg ctataacact taatactgat gctaatacaa
     7921 gaaaggtatc ctcttcaatc acatttacaa taatggagct tgtaaggagc attctgccgg
     7981 ccagctttgc ttcagcagta cccgcccttt cccgcccctc ctgcggcagt acgatttctg
     8041 tatccatatc caactcctcc ccagtaccat tatattattg tacatgttct gtgtgcttct
     8101 tgaatcctcc attagggctg ctgcacaata gaagaatgat gcgacacaac tataagtttg
     8161 cagtcatact cccagagcac aagccttgta tcccgactcg cacgatatta gctttggaca
     8221 gaatcagagc ttgatacttg taaaatcctg acgcacataa acccaacctc ctgttggtat
     8281 gtgctggatt tacttcacgt aaatcccacg gattttactg cgcatcttct ctcgattctg
     8341 ttgcccaaag ccaataagtg ctcactgtta tataaggcta gtgctctgca ggataactac
     8401 aaacttcaga tacaacttac acggattatg attttaaagt tactagtgtg ccctgctata
     8461 ctagacaagc tcttgcttgg ttatacattc ttcagaatac tgccttgtag ataaaggata
     8521 tgcaaatacc gacgtgggga agatatcttc gacgaaacct tccacctgac cccggaacgg
     8581 gaatacctcc aggcaaaata accagacggg cccgcagaga tgggaccttc gccagtgatt
     8641 ttgctggctt tggaaggtga ggagaacata cttcccctac agtcggagta cttcacaaac
     8701 ctgtatatat tgcgcattat tcttcttata tgtctttcag gtttagtact cttatggcat
     8761 tcaataccgc tatcagcgca actcccacat cagcaaatac cgcttcccac attgttgcca
     8821 atcctccggc acccagaagc agaacaatca gcttaatact cattgcaaat acaatattct
     8881 gcagtactat cctcctggtt ttctttgcta tccttatagc gctgacaatt ttggagggct
     8941 catccgtcat tatcactata tccgctgctt caattgccgc gtcggagccc aggccgccca
     9001 ttgcaattcc gatatctgcc ctggccaata caggggcatc gttaataccg tctcccacaa
     9061 aggcaacctt gcccccggac ctcatctcat tcttaatcca ttcaagcttt tcaacctttt
     9121 gatccggcaa taactccgaa tacacctcgt ccagtcccag cgccgaccct gtcttctctc
     9181 ctggcgccct ggcatctccg gtaagcatta ttgtttttct tatgcctgaa gccttaagga
     9241 ggagaatagc tcttgccgca tcatctttta tctcatcaga aattactata tatccggcat
     9301 aagctccatc tactgctaca tgcacaagcg ttcctgcaat atcagggttt tctgctgata
     9361 ttccctccaa ttgcatcatt tttatatttc ctgctgctac ccttttcccg aaggcctctg
     9421 ccctgacccc atggccggaa atctcctcgt aattcgaaat ttcactgccg attatctcac
     9481 tgccgtatgc cttaagtatt gataatgcaa tgggatggcc ggagtagctt tcgatatatg
     9541 ccgcatattg caatagctct ccttccttca tttcacccag tgccttgatt tcagtcactt
     9601 taaagactcc ttttgtcagt gtgccggtct tgtcaaatac aatggtatcc acctggttta
     9661 atgcatcaag gtagttactt ccctttataa gcactccgtt tttcgaagca cctccaatac
     9721 ctccgaaaaa ccccaaaggt atggatatga ccaaagcaca gggacatgaa accacaagaa
     9781 atatcaatgc cctgtaaatc cagtctgaaa aagcttctcc ctgcagcacc aaaggcggaa
     9841 caaatgcaag agctaatgcg gaaaacacca ctgcaggagt gtaatacctt gcaaacttgg
     9901 taatgaagtt ttcggtgggg gattttttac tacttgcgtt ctctaccatg tcaagaatct
     9961 tggataccgt cgattctcca aactccttgc tcacttcaac ggtaagaaca ccgctgttat
    10021 ttataaagcc tgccagaatc tcatccccgg cattggcctc ccttggcact gactctccgg
    10081 tcaaagccga agtatccaac attgatctgc cttttgttat tctgccgtcc agaggaaccc
    10141 tttcccccgg ttttacaatt atataagctc ccaccggcac atcttccggg ggcacctgta
    10201 tcatctggcc ctcagagacc agattggcat aatcaggccg tatgtccatc agttctttta
    10261 tcgaccttct ggaacgatct acagctaaat cctggaacat ctcccctatc tgataaaaaa
    10321 tcattaccgc aacgccttcg gagtattctc ctattgcaaa tgctccaata gttgcaagac
    10381 tcataaggaa gttctcatca aaaacttgcc ccctgaaaat attccttatc gatttgacaa
    10441 gtacttctcc tccgaccaat agatatgctg tcagatacat agccaattcc acgcctgcag
    10501 aaagcttaag caaaacagcc gcagcaaaaa atgcggcacc aataccgaat tgaaacggct
    10561 tcagcctttt tatccctgat tcattttttt cttctgaatc tgcttcccag gcctttctgt
    10621 ctgttatctc tttcagcttt acatcaggct taatattcct tacgatttgc tttacttcct
    10681 ttattgctgc cgccgcatcg gcagattcga atatctcaag cttcagcttt tttgtcacaa
    10741 aatctataga tgaattccta atcccttcca gggatttgat gccaagatcc atcttgtcag
    10801 cacaaactgc tcagccaagg cctgtaagta tgaattcttt tacaacatat tcatcgtttt
    10861 tttcaaaatc tgctgctgct tctcttttca cattaatacc cccgtctcac gctttgttta
    10921 atgctatctc tccctgatat gcaccagtcc ttggtcgaat atctgtttta catgttcatc
    10981 atccaaggaa taatatacta ctttgccttc ttttctgtat ttcaccagcc ttgcctgctt
    11041 caaaaccctg agttggtggg atattgcaga ctgattcatg gataacagtg ccgctatatc
    11101 acatacacac atctcagatt caaacaacgc gcacaatatt ttgattcttg ttgtgtcgcc
    11161 aaaaaccttg aacaactcag ccaggtcata gagcgtctct tcttcaggca tactgttcct
    11221 tacctttgac acagcatctt catgtatcat ggaacagctg cagctttcaa tacttaccct
    11281 gttttcttcc atagcgccct ccatattatt atatatgaac atatgtttat gtgttcatat
    11341 gttcatatat atattactgc tttaattcat aaaagtcaac aacaaagagg gacatgggga
    11401 cgttcgggac atggggacgt ttctcctgtc ccgctgaaag cgggacagga gaaacgtccc
    11461 catgtcctca aaaaacaatt gtaaccaaaa gcagattaca acataaaata tgaataggat
    11521 gatgaacaac ctcttctgca gaaggaggtg taattatggc gagaggtttt aaccatggtt
    11581 tgtatggatt gaataagccc ggctgagagc cgggttcagc accggaagat ttttcgggag
    11641 tattttactc aaaatcaaga aagcagcaac tctctgcggc agttcccccg ccacccccgg
    11701 aaccggcccc ccctgcagca attcccccgc ctcaaaatct gggcggaatg tttaacgggc
    11761 ttttgggaaa tgtgtttgag aataataatt ggtggttgtg ggctgcttta ctttttatct
    11821 tctttaatcc cgacctgaga aaaaaactca taggaaagtc aatagaggct gaccgggacc
    11881 cttagtcagc ctctctcaat cccaagcctt ttctcttgga aatgatatgc gcttctatat
    11941 tgtttgccgc ttccgccgga tcatccccca gtgcaatctt tccccctgtc agtccttcca
    12001 catcttccgt aagaagctta accaaccccg gagcccccgt gacgaatggt attggcgaaa
    12061 ggcgggtata ggcaccgtag gctgctgcaa agaagccgtc gatggtagcc ttctgctcca
    12121 tccattcagg tgccgttacc gctatgggaa gctgcggtat atcgacatcc agatggtctg
    12181 ccatttcacc tgccattttg ttcacatcct gcttgctgtc aatccctacc gtatcgcaca
    12241 tccatttaag cttatttgca tcggtaatct taaagggagt tttcccctgg gcggtctccc
    12301 tcagggttcg gaaagcctca taggcatgat ggctgtacgt acctgccccc atgatgtttc
    12361 tcagaagcat attcctcatt gccattgtgt caccgcctat tccgcataca cccttatcct
    12421 gtccgctttt ttcactgatt ctgcaagggc cgttggtaca aagctggcag cttaatccct
    12481 gaaggcaaaa tttacagcgg atcttttcct gctgtgcata tctgtcaaag gcattggaca
    12541 ttccgtcttc ccttattcga ggaagcatct cctctcccga atcatgatag ctgacccgtc
    12601 cttcagtctt ttctgaaata gtttctccca ttgataaatt ccttcctttc aagcatgttt
    12661 cagagtaagt tttctcataa agcagggtaa atatgtgctt aagcactgta taccgaataa
    12721 aaaaagcatg atacttattt aagcatcatg cccatttttt attctcctga ctccaactca
    12781 taaggccaat ccattattcc gcccatattg tataccttgc tgtatccaag ccctgccaat
    12841 atgttagcag cactgctgct tcttttgccg cttgcacagt atacgataat atccgcatcc
    12901 ttatcagtca gctctgcttc cgctctcttc tccaaatcat taaccggtat taaaatactt
    12961 cccggtatat gttgttcttc atattccccc tgggtcctta catctaacag tataatatcc
    13021 tcttccgatt ccagcctttt gtatgcctct tcaggagaaa tgctttcggg ctgaactact
    13081 tctgcaggac tttgttcggg ttgaactatt tctgcgggac tttgagagat gtcctgctgc
    13141 ggttttgtac tgcaggctga aaatactgcg gcccccaata atactgccag caataatatt
    13201 actattctct tcattcgctc caccgtcctg ttacaggctt cccttcaaac caatttcttc
    13261 tgcagctttc ttcattccat taattgtttc tccgataata gtgtccattt ccatgcccag
    13321 aagcgctgct ccctcttcta taacctccct gttgacgcct gccgcaaagc tcttttgctt
    13381 ccatttcttt tttacggatt ttacttccat atccaatatg cttctgcttg gcctcatgat
    13441 ggcagtcgct gcaataagcc ctgtcagctc atcaatagta taaagtattt tttccatttt
    13501 ctctgtcggt tctacgtcgg agcaaagctt ccaaccgtgg ctcagcactg catgaatata
    13561 atcttcaggc catcctcttt cggtaagtat ctcttttgct tttatacaat gctgttccgg
    13621 gtacatttca taatcaatat catgcagcag gcctataatg ccccattttt cctcatcctc
    13681 cccaaggagt ccggcaaaat gcctcataac ggattcaact gtcagtgcat gcttcaccag
    13741 gctgtcattc ttgttatact cctgcaggag ttccaatgcc gctgctctgc tcggaattgt
    13801 gtttaccata gaaaatcctt ctttcgtatt gtttttttgt ttgcattacc tgccggcttt
    13861 tttctttagt acggccttgg tgcttttcgc tatatcggct gccgtcaggc catacatttg
    13921 gagtaaaata tccggtttgc cggactttcc gaacttgtcc tgcacaccaa cccttacaag
    13981 gggtacaggg taattctccg aaagtacttc agccactgca cttccaaggc ccccgataat
    14041 attgtgctct tcagcagtga cgatagcccc tgtttccctg gcagctttca ctattatatc
    14101 cgcatcaata ggctttatgg tatgaatatc tattaccctt gcatttattc cttcctgctg
    14161 aagcagttcc ttcgcttcaa gagccgcact caccataagc ccggtagcaa taattgcagc
    14221 gtcatttcct tctgcaagta caataccctt gcctatttcg aacttatagg agctttcatt
    14281 atatattaca ggaacgttca atcttcccaa tctgagatat acaggcccct catgctctat
    14341 tgcggccttt acggcatatt tggtttcaac agcgtcagca gggcttatta ccgtcatatt
    14401 gggaagcgcc ctcatgattg caatatcttc aattgcctga tgcgatgccc cgtcttcccc
    14461 tactgataaa ccggcatggg ttgcaccgat ttttacattt aattttggat agcatattga
    14521 attccttatc tgctcaaagg ccctccctgc ggcaaacatg gcgaaggtac ttgcaaatac
    14581 caccttgccg caggaggcca ttcctgcagc cgtagccatc atattgcctt cagctatgcc
    14641 catattgaaa aacctttcag ggtatttttt tctgaaatta tctgtctttg tggattttga
    14701 caagtctgca tcaaacacca ctattctgtt gtcggagcca aactccgcaa gtgcatttcc
    14761 gtatgcttcc cttgtagcta tcttatctgc catactattc aacctccaac tctgccagta
    14821 ctttatccag ttccgccatt gcctggtctc tttgttcctt gtttggagca ctgccatgcc
    14881 agcctgcctg gttctccata taagaaaccc ctttcccttt gacggtctcg gcaattatta
    14941 ttgtaggttt gcctttacaa tccttggcct gccctataga ctcaagtatt ttttcaatat
    15001 catggccatc ggtcttaagc acattccaac caaaggcttt aaatttatcc gctacaggtt
    15061 caggagacat tacatcggta atcctgccgt caatctgaag cccgttatgg tccagaaaag
    15121 ccgtcaggtt atcaagcttg tagtgagccg cagccataaa agcttcccag acctcgcctt
    15181 cctgaagctc tccgtcaccc agtacggcat agaccctgca gtccttctta tccagcttgc
    15241 cggccatagc catgcccaca gctgccgaaa tgccctggcc caaagacccg gtggacatat
    15301 ccaccccggg tacatatctc atgctggggt gcccttccag atagctgtca gctttcctga
    15361 aggtcaaaag gtcctcttta gggaaaaagc ccttttctgc cagtaccgca tataaggcag
    15421 gggagcaatg gccttttgac agtacgaacc tgtccctgtc ctcccataag ggctgtttag
    15481 tgtttacacg catttcagcg aaatacaata ctgttaaaat ttcggtacaa gacagggatc
    15541 ctcccggatg tcccgatgca gcgctgtaaa cttcgtctat gatgtgcttt cttactttga
    15601 ctgcatactt ttttagatct ttaatttcag gttttttcat ttgctgcctc ctagtatttt
    15661 ataaaattat agcttccata tatttaagcc gcaatagtca tcaaataccc ttcctatttc
    15721 tccatcaact gcatctataa taagctcaca ggtcccggaa atccagcact gattcaaatg
    15781 ccccccatga gcattatatt cctcatcgcc caatgttata tgcagatgaa ggtatacttc
    15841 accatctttt tcagttatgc ttcctgtcag attagtgatc tcatattctc cggtaagctc
    15901 ttgattatga tattccttcg taaccgtgtt gaagacacct atcgtaatcc tattagctgc
    15961 cccgatacca atgacagacc ccaacttcaa accgttatcc gtgcaaaatt tatataatga
    16021 cgcaactaac tcttcaccct tatcaattct cacaaaatac ttactgccta tttttgtatg
    16081 cttcatattc tcaccctttc caaatagatg catcgaactt ttgaacttat tatgacccaa
    16141 atattctttt atgtcaatat taccgaaata gggagtattt atctctccgt ccccccgggc
    16201 attctccatt ttctctacaa aaatccgctt ctttagtgta taatgatata gtgattttaa
    16261 tatttggagg atactatgat aaagattgat aatgatacgg taagatttga aggctacgaa
    16321 ttggaaaacc ttgtggtttt attggaacag gtagtaagaa attcgattat agattccgac
    16381 agcagggaat tcgcgatctc cttcttaaaa gctttcagcg attgcttcgg tgaagatgtt
    16441 attaagaagt tcaattcaat cagaaaatag aattctcata tatgaagaat aatgcgacgg
    16501 ggacatgggg acgtggggac acggggacgt ttcgcgcggg gacacgggga cgtttc
//
LOCUS       DAOQUR010000047        16493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_3995_length_16493_cov_9.252038, whole genome shotgun sequence.
ACCESSION   DAOQUR010000047 DAOQUR010000000
VERSION     DAOQUR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3995_length_16493_cov_9.252038"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKI50_09370"
     CDS             complement(<1..261)
                     /locus_tag="PKI50_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNT03949.1"
                     /translation="MKTIDFMGPRKIWFGISLALLAVGLIFLIINGGLNLGMDFVGGS
                     SIHATIGKTFDVSEIREIMDKYDPEATITHAGQNKDQVIVRTK"
     gene            complement(263..1528)
                     /gene="secD"
                     /locus_tag="PKI50_09375"
     CDS             complement(263..1528)
                     /gene="secD"
                     /locus_tag="PKI50_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNT03950.1"
                     /translation="MKSKSLILFLAIVLLISFFAYIAFFGLNIGKYEIVPAKDLIKLG
                     LDLRGGVTVLLEAKDKPDDPVNDEKMARAVATIRERIDTLGVTEPIITRVGADRIEVQ
                     LPEIQDPQRALDLIGQTAQLEFIEEETGTVILTGNDVKKAQAEFAPSKDGLGQTPVVA
                     FELSPEGAKKFKEATERNINKIIGIYLDKNPISLPKVESVIPDGKGIITGSATMEEAG
                     DLATLIRAGALPVPLETLSVTAVGPQLGANSFEQSVNAGQIGILLVFIFMIFYYRIPG
                     LVADITLVLYLTLTLFALAAMKATLTLPGIAGLVLSVGMAVDANVVIYERIKDELNLG
                     KTLRAAIESGFRRAFLTIFDSNLTTIIACVILFYFGSGPIRGFAVTLIVGVAVSMFTA
                     LTITKIMLKLLTSGGILKSMRYFGERGVK"
     gene            complement(1693..2208)
                     /locus_tag="PKI50_09380"
     CDS             complement(1693..2208)
                     /locus_tag="PKI50_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNT03951.1"
                     /translation="MIISYKHYKPNIGKNTFITENSTIIGRCIIGENCSIWYNTVVRA
                     DVNEIIIGRGTNIQDGCVVHCANDYKTIIGENVTVGHNAIIHGCTVGNNCLIGMGAII
                     IDGAVIGDNVIVGANSLITSGKKIPPGVLVMGSPAKVVRELAPAEIEGISHSAEEYII
                     LSKEYSKLSIE"
     gene            complement(2311..2496)
                     /gene="scfA"
                     /locus_tag="PKI50_09385"
     CDS             complement(2311..2496)
                     /gene="scfA"
                     /locus_tag="PKI50_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HNT03952.1"
                     /translation="MKHVKTINKRSLYDSVQKGGCGECQNSCQSACKTSCTVANQKCE
                     NIQNSRVRTGTSHKNTI"
     gene            complement(2594..3007)
                     /locus_tag="PKI50_09390"
     CDS             complement(2594..3007)
                     /locus_tag="PKI50_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HNT03953.1"
                     /translation="MRKFKSYTKMPPAEEKGINIYGVFLKSLGICFGVSFILIMLYAL
                     VLSFTSMSDSSMSMTIQIIMIVSITISSIYGGKKILKKGWFFGIVLGLVFTLLLVPLG
                     MCFGQSLSLDKYFAAKMLMGSSVGLIGGIIGINLN"
     gene            complement(3131..3406)
                     /gene="yajC"
                     /locus_tag="PKI50_09395"
     CDS             complement(3131..3406)
                     /gene="yajC"
                     /locus_tag="PKI50_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNT03954.1"
                     /translation="MQQFGWILPYAIVIAAFYFFLIRPQKKKEKATQEMRNSVKEGME
                     IVTIGGFYGKVVNAKEDVLTIEVGADKVKLKIARWAVGKVIENNEEK"
     gene            complement(3571..4689)
                     /gene="tgt"
                     /locus_tag="PKI50_09400"
     CDS             complement(3571..4689)
                     /gene="tgt"
                     /locus_tag="PKI50_09400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNT03955.1"
                     /translation="MTIKYELIKEEKRTGARLGKLHTPHGVIDTPVFMPVGTQATVKA
                     MSPLELKETGAQIILSNTYHLYIRPGHRLVEKAGGLHGFMNWDRAILTDSGGFQVFSL
                     NELRKISEEGVEFKSHLDGSRHFFTPEYVMEIENSLGADIIMAFDECAPYPCEHDYAK
                     KSMERTARWLKRCKEAHNNTEKQSLFGIVQGSVYKDLRIESAKRTVEMDLPGYAIGGL
                     SVGEPKELMNEVLEYTVPELPSDKPRYLMGVGSPDCLIEGSIRGIDMFDCVLPTRIAR
                     NGTVMTSRGKLVVRNAEYSEDFRPLDEECDCYACRNFSRAYIRHLIKAGEILGGRLTT
                     VHNLRFLQNLMSEIRKAIMEDKLTELRNDFFEKYGYEK"
     gene            complement(4727..5359)
                     /locus_tag="PKI50_09405"
     CDS             complement(4727..5359)
                     /locus_tag="PKI50_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNT03956.1"
                     /translation="MKMTRIKHSIINASLYLENILAIVIILAIVIGMIDLAKYIVLIF
                     QTSTLQTYEVFQKFLGHTLLLVVGVEMVAMLVRHTPGSVIEVLLYAVARKMLISNAHM
                     LEFFLGIVSIASIFAIKKYLFISNLRDSEAINIFPASASISDINNTIGVKIPEDLADT
                     VGGLISRLSKQSCRDIHEGSYYRIADAELKVLSYNDGVIHRVWAAKYNES"
     gene            complement(5377..6402)
                     /gene="queA"
                     /locus_tag="PKI50_09410"
     CDS             complement(5377..6402)
                     /gene="queA"
                     /locus_tag="PKI50_09410"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNT03957.1"
                     /translation="MELKDFHYDLPEELIAQEPIPERDMSRLLVLDKNTGEIVHRSFR
                     DILEYLNEGDCLVMNNTRVIPARLFGVKRGSTGNMEFLLLNRKDKYVWEVMVRPGKRA
                     KIGAEFIFGDGELSGEIIEILEGGNRLVKFNYEGIFEEVLDRLGKVPLPPYIKKELTD
                     RERYQTVYSKIEGSAAAPTAGLHFTNELMHQLAEAGVNIAYLTLHVGIGTFRPVKVSN
                     IEEHKMHSEYYELTAESAEIINNTRKNGGRIICTGTTSARTLESIMQRKGSLKEDKGW
                     TDIFIYPGYQFKITDGLITNFHLPESTLLMLVSALAGRDNIMRAYKEAVKEHYRFFSF
                     GDAMLII"
     gene            complement(6417..8060)
                     /locus_tag="PKI50_09415"
     CDS             complement(6417..8060)
                     /locus_tag="PKI50_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT03958.1"
                     /translation="MKSKKFAVLYLVITSVMFMILHADIAVPETIRIGLSFGQSQANI
                     FTLASETGMNILILEKGSYKNLLEVKNPKVVKVRRDEYYNIAGGKECEINYVRAAKYE
                     GEVVGPYHIQIGGVYANAEAALQVLKQVSSITQSVFLAYEEGWRVWSQLYLDESECLE
                     QIKIMKKEASDLKYSVVYPDKKRIQIIDNTTGKLMLLLNSEGNVKVTPKEVNGKVSSL
                     QYKGKKYRGSIVMQSFEESDITVINELSLDQYLYSVVPSEMPASWHIEALKAQAVAAR
                     NFALVTMGRHNAHGFDLCSTEHCQAYNGLAQENSNSTEAVNATKGKVITYNGVLISAF
                     YHSSSGGHTEDSENVWGAKTDYIRGVEDKFSLGSPHDNWTVELNRAELKEKLAQSGVD
                     LGEIRDVRILECSKFGRVTKLEVKGSKDTTVFEKEKIRSILGTRLLKSIWYDLKTDAD
                     VFVRGSILNSAEKGRTSNMYVVSASGKAVVKGKGNKVSVKGMNDTKAYNVIPDKYTFD
                     GKGFGHGLGMSQYGAKGMAEAGYNYQKILEHYYQNANVQ"
     gene            complement(8095..8310)
                     /locus_tag="PKI50_09420"
     CDS             complement(8095..8310)
                     /locus_tag="PKI50_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNT03959.1"
                     /translation="MNFQYFAKIIIVLGIGMVIFGTLLYFGGKLGFGRLPGDIYIKKG
                     NFTFYFPIVTCILASIIMTIVFGFIRR"
     gene            complement(8307..8852)
                     /locus_tag="PKI50_09425"
     CDS             complement(8307..8852)
                     /locus_tag="PKI50_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNT03960.1"
                     /translation="MKLLLHMCCGPCAVYPHQVLSREHEITGLFYNPNIHPYTEYKKR
                     LDTARDFADKSGYKLITIDEYNLDEFLRNAAFREGQRCIMCYAERLERAASVAKSGCF
                     DAFTTTLLVSPFQKHDLIRRIGEEAGKKYGVEFLYKDFRGGFKEGVEKSRELGLYRQP
                     YCGCIYSERDRYMPGRKVGRK"
     gene            complement(8868..9863)
                     /gene="ruvB"
                     /locus_tag="PKI50_09430"
     CDS             complement(8868..9863)
                     /gene="ruvB"
                     /locus_tag="PKI50_09430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNT03961.1"
                     /translation="MEDRLITPKIIEDDIEIDTSLRPKSLEEYIGQSVAKEKISIFIQ
                     AANKRKETLDHVLLYGPPGLGKTTLANIISNEMGVSIRITSGPAIEKPGDLAAILTNL
                     DNNDVLFIDEIHRLNRSVEEILYPAMEDYALDIIIGKGPSARSIRLDLPRFTMIGATT
                     RAGMVTAPLRDRFGVICRLEYYTDEELFKIIKRSARILKIDTDEAGAFEIARRSRGTP
                     RVANRLLKRVRDFAQVKASGVIDKETADRALKLLEIDDMGLDDIDKKMMLTIINKFDG
                     GPVGLDTLAASIGEDSGTIEDVYEPYLLQIGFINRTPRGRVATNSAYRHFGIKKD"
     gene            complement(9893..10480)
                     /gene="ruvA"
                     /locus_tag="PKI50_09435"
     CDS             complement(9893..10480)
                     /gene="ruvA"
                     /locus_tag="PKI50_09435"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNT03962.1"
                     /translation="MYEYLSGRVDYIGEDYAVIDISGLGYRVFTSQNSMKSLKLGEIS
                     KIFTHLIVKEDDLILYGFATREELGMFKLLISVSGVGPKAAASLLNQYKASELAAAIL
                     SRDVAKLSKAQGIGKKISERIILELKDKIDTESAVGAIGAFGGSDEISQVIEALTALG
                     YNYSIAANAVMKLKDTNKPIDILIKEALRQLGTMK"
     gene            complement(10493..10984)
                     /gene="ruvC"
                     /locus_tag="PKI50_09440"
     CDS             complement(10493..10984)
                     /gene="ruvC"
                     /locus_tag="PKI50_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNT03963.1"
                     /translation="MLILGVDPGIAILGYGLVKYEANRFTVIDYGAITTDSGINMSAR
                     LTIIYDRLVDIIERHNPDAFAIEELFFNKNIKTALTVGHARGVAVLAGSKSGVQVYEY
                     TPLQVKQAVVGYGRADKNQMQQMVKVILNLGEIPKPDDVADALAVAICHGNSSKFSEQ
                     FRV"
     gene            complement(11436..12095)
                     /locus_tag="PKI50_09445"
     CDS             complement(11436..12095)
                     /locus_tag="PKI50_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03964.1"
                     /translation="MFEDRSIRRKRRYLLAAAFLLLISAAFAVGYFFNADIPEALDNP
                     DNQPSANLQIPDSLINPTTVRKPYEEVNAESNTEETANILEIDFPTPNTQLIFKTYYS
                     SCRHTVEKAVQAGSDEINMNEQQLKEKYSHWHLSGYSPPVVEFSRNIDTYCPGHYIIG
                     VDGGYIAIYVYDENGHKSLQEKTDISAAPLTPEDQQALAGGIVVDTEEQKEQTLEGFS
                     N"
     gene            12300..13184
                     /gene="uvsE"
                     /locus_tag="PKI50_09450"
     CDS             12300..13184
                     /gene="uvsE"
                     /locus_tag="PKI50_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HNT03965.1"
                     /translation="MTGLGFACISKSLKENGRFRTMSVKTYNSLKPDERIRRLRSISV
                     DNLYNTCRIILWCIENSISLYRLSSGLIPLAGCVPEWKWWEDRDIRNMCEKIKKIVDE
                     GKIRISMHPDQFCVLNSDKAEVVKGSIAILEHHNRLSNMVGNRILVIHAGSGTGGKSK
                     AIERFIKNFNMLDEDIKKKIAIENDDKVFNAEDILHLCEHLGVPMVLDIHHHNCNHEK
                     DNLPQLMDRIKSTWKGKRPKVHLSSGKTCPTDRHHADYIDFGDYRQAAELVKDEFDIM
                     MECKEKDLAVLKILTYCI"
     gene            13279..15210
                     /locus_tag="PKI50_09455"
     CDS             13279..15210
                     /locus_tag="PKI50_09455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNT03966.1"
                     /translation="MFINGKIPHLLQQTVLMTAFTLLKFTALYHFAGKPQDMLPAALR
                     TYTLLFAIYLLTNLVIGRKSFLIRIAFHEFLALIVLADTLYYKQFNILPEVADLQFLK
                     VLPTIWDSVGFLFSGIHLLLFADALILMTHQYALNRQEKASAPGILPSVIAAALLLAI
                     IFTDFSFSDIKSSGQAYEKFGLLHYHISQIPGVLQSNSNSSSRSIGKAQIPAEVEEAA
                     KEPRCFGIAKGRNVIVIQVESLQDFVINMHYEGQELTPNLNKLLLKDTIYFNRFYQQL
                     GKGNTSDAEFVTQNSLYPSIDIPAYRKYENNRFMGLPMIMRETGYGTYAFHAYKPDFW
                     NRQNMYPMLGFDRFINMNDFNLNETFGMGLSDKEFFEQSAAYISTANQPFYSFLVTLS
                     SHHPFGLPQKYKKIQLQPAHKDTLFGRYIQVINYTDEAIGLFLEKLKEYGLYDNSIIA
                     LYGDHRSIPNGVPENDKLMSELLDHEYTFDESLNVPLIIHIPGTNIIETNTIVGGQMD
                     FLPTMLNLLGIKEIRPKLFGQDLLNAKNGFVASQTGMIKGSFIDNEKIFVMSRDGVFE
                     NSTAWKLDTREPVDPEFCRNGYERALREIKKSEYILANDLIHEFITLGKSHTTFSSAI
                     EAASGYIERIAALFKSIVE"
     gene            15368..16060
                     /locus_tag="PKI50_09460"
     CDS             15368..16060
                     /locus_tag="PKI50_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03967.1"
                     /translation="MKKSIVLLSILALLAAVFAAVAAKPLKIGDVIGKIYYTDIKTYI
                     DGKLIESVNIGGRTMISAEDLQYMGYLVEWNDTARTLNITSNTSHNISGKVIIPDGKV
                     APKGGLNVEIAAFINPNPDPSVISRGYGPEFGKEIRLVIPEGHSSIEYIISVPYKFEP
                     RYITAILPGGESGEPEKLPDIKPYYVLRFRYSYYLGNHKVEHGDFMEGYLDMSTGDKT
                     GVDYTFDNIDLK"
BASE COUNT         4499 a         3910 c         2998 g         5086 t
ORIGIN      
        1 cttggttctt actatgacct gatccttgtt ttgtcctgca tgagttattg ttgcttccgg
       61 atcgtatttg tccataattt ctcttatttc ggatacatca aaggttttcc cgatggtagc
      121 atgaatagag cttcccccta caaaatccat acccagattc aaaccgccgt taatgatcaa
      181 aaatatcaat cctacagcca gcagggcaag tgatattcca aaccatattt ttctgggtcc
      241 cataaagtca atggtcttca tattacttca ctcccctttc accaaaatat ctcatgctct
      301 ttaatatgcc tccgctggtc agcagcttca gcatgatctt tgtaatagtc aatgctgtga
      361 acatactgac agccacgccc acaatcaggg taactgcaaa gcctcttatg ggtccggatc
      421 caaagtagaa cagtataaca caggcaatta ttgttgtcag gttggagtca aatatcgtaa
      481 ggaatgctct cctgaaaccg ctttctatgg ctgccctcaa agtttttccc aaattcagct
      541 cgtcctttat tctttcatat attactacat ttgcatcaac tgccataccc actgaaagaa
      601 ccaaacccgc tatacccggc aaagtaagtg tcgctttcat tgccgccagt gcaaataatg
      661 taagagtcaa atagagcact aaagtaatat ctgcaacaag gccgggtatt ctataataga
      721 atatcataaa aatgaaaacc agtaatatcc ctatctgccc tgcgttaaca ctctgttcaa
      781 aagaattcgc ccccaattga ggccccactg ctgttactga cagggtctcc agcggtacgg
      841 gaagcgcccc tgccctaata agcgttgcaa ggtctcctgc ctcttccatg gtggctgagc
      901 ctgtaattat accttttccg tcaggtatta cactttctac ttttggaagt gatatcggat
      961 tcttatcgag atatatccca attattttat taatgtttct ttcagtagcc tccttaaact
     1021 tctttgctcc ttccggtgac agttcaaagg ctactaccgg agtctggcct aatccgtctt
     1081 tgcttggtgc gaactctgcc tgagctttct ttacgtcatt gcccgtcagt attaccgttc
     1141 ctgtttcttc ttcgataaat tccagctgag cagtctgtcc gatcaagtcc aaagccctct
     1201 gcggatcctg tatttcagga agctgcacct ctatcctgtc tgctcctact cttgtaataa
     1261 taggttctgt tactccgaga gtatctatac gttctctaat agttgcaact gctcttgcca
     1321 tcttctcatc atttaccgga tcatcgggtt tatcctttgc ttccagcaga acagtcactc
     1381 cgcctcttaa atcaagaccc aatttgataa ggtctttagc cggtacaatt tcatattttc
     1441 ctatattaag cccaaaaaac gctatatatg caaaaaatga aattaacaaa actattgcaa
     1501 gaaacaatat taggctcttt gacttcattt gcttattccc ccttatttga tttcacaatg
     1561 gttattatat tacttttaca atttttagtc aatgaagaga agtattccca ttgcgaacat
     1621 caggcttcca tatgtattat ttgctgcttt tcttatttta tccaatctgc aaattactgc
     1681 ttaaagcttt acttattcta ttgacagctt tgaatactct tttgaaagaa ttatatattc
     1741 ttctgcagag tggcttatac cttcaatttc cgcaggagcc agttccctta caacctttgc
     1801 aggactcccc ataacaagaa cccctggcgg aattttcttg cctgaagtaa tcaatgaatt
     1861 ggctcctact attacattgt ctccaataac tgcaccgtca attataatgg ctcccattcc
     1921 tatcaggcaa ttgttcccta ccgtgcatcc atgaatgatt gcattgtgcc ctacagtgac
     1981 attttcaccg ataatggttt tataatcatt tgcacagtga acaacgcacc catcctgaat
     2041 attagtgcct cttccaatga ttatttcatt tacgtccgcc ctaaccaccg tattatacca
     2101 aatgctgcag ttttcgccta tgatgcatct gcctataatc gtgctgtttt ctgttataaa
     2161 tgtgtttttc cctatgtttg gtttataatg cttgtacgat attatcatat gccctccatc
     2221 acacatgttt taagcagtaa cataaagggt ctgctctaaa gtatcccctt atattactgc
     2281 ctatgtacca tagctatata tgatattcca ttaaatcgta ttcttatgtg aagttcccgt
     2341 cctaaccctg ctgttctgaa tattttcaca tttctgattt gctactgtac aggaagtctt
     2401 gcacgctgac tggcacgaat tctgacattc accgcagccg cccttttgta cgctgtcata
     2461 taatgatctt ttgtttatcg tttttacatg cttcattctg tttcctcctc catgttttgt
     2521 cactgttatg attatagcat attagttttt tatgggaaag aaaaatctgc cttcgtatca
     2581 acaaaaaacc ggctcaattc aggtttatcc ctattattcc tcctatcagc ccaacagaac
     2641 tccccataag catcttcgcc gcaaaatact tgtcaaggct cagactctgt ccgaagcaca
     2701 tacccaaggg taccagcagc agtgtaaata ccagtcccaa tactattcca aaaaaccagc
     2761 ccttcttcag aatcttcttg cctccgtaaa tacttgaaat tgttatgctt acaatcatta
     2821 ttatctgtat tgtcatgctc attgatgaat cactcatact tgtaaacgac agcaccagcg
     2881 catacagcat tatcagtatg aaagacacac caaaacatat tccgagtgat tttaagaata
     2941 ccccatatat attaattcct ttttcttctg caggcggcat cttagtatag ctcttaaact
     3001 ttctcatgaa aaataacccc ctatagtatt tttacaaggt ttgttttcta taaatttatt
     3061 cgctataagg gacatttatt ctcatatgac gaaaattcag gggaatgaag aaaagtaact
     3121 tggatccaag ttacttttct tcattgttct ctattacctt tcccactgcc catcttgcaa
     3181 ttttgagctt tactttatca gccccaacct ctatggtaag tacatcttcc tttgcattaa
     3241 ccacttttcc gtaaaatccc cctatggtta caatttccat accctcttta acactgtttc
     3301 gcatttcctg cgttgctttc tccttcttct tctgtggtct tatcagaaaa aagtaaaaag
     3361 cagctataac aattgcataa ggtaatatcc atccaaactg ctgcatactt tacattcctc
     3421 ctttcatatc ttattccacc ggcataatta ttccgaaata gctcctaaat atgaatgcaa
     3481 gcttaaacac cagtggctgg tattattctc tgaattatac tattcaatat agaagtcaaa
     3541 aatccttccc gctatacaac ttatactata ttatttttca tatccatact tttcaaagaa
     3601 gtcattccta agctcagtaa gcttatcctc cattatggct tttcttatct cagacatcag
     3661 attctgaagg aacctgaggt tgtgaacggt ggtaagcctt cctcccagta tttcacctgc
     3721 cttaataaga tgtcttatat aggctcttga gaagtttctg caggcataac agtcacattc
     3781 ctcgtccaag ggcctgaaat cctcactgta ttctgcattt ctgacaacca gctttcctct
     3841 tgaggtcatc acagtaccgt ttcttgcaat tcttgtaggc agcacgcagt caaacatatc
     3901 tattcctctt attgagcctt ctatcagaca atcagggctt cccaccccca taagatatct
     3961 gggtttatcg gagggaagct ccggcactgt atattccagc acctcattca ttaattcctt
     4021 cggctctccg acgctcagac ctcctattgc ataccccggc aaatccattt ctacagttct
     4081 tttggcactt tcaatcctca aatccttata gacacttcct tgcactatcc cgaaaagaga
     4141 ttgcttctcg gtgttattat gtgcttcctt gcaccttttc aaccatcttg cagttctttc
     4201 catggacttt ttcgcataat catgctcaca gggataaggt gcacattcgt caaaggccat
     4261 tataatatca gccccaagcg aattctcgat ttccatcaca tattcgggag tgaaaaaatg
     4321 cctggaccca tccaggtgtg atttgaattc cacaccttct tccgatatct tcctcagttc
     4381 attgaggctg aatacctgaa atccgccgct gtcggtaaga atagccctgt cccaattcat
     4441 gaacccatga agtccccctg ccttctctac cagcctatgc cccggcctta tgtacaaatg
     4501 atatgtattg ctgagtatta tctgcgctcc ggtttccttc agttcaagag gagacatagc
     4561 ctttaccgta gcttgggtgc ctaccggcat gaataccgga gtatcaatga ctccatgggg
     4621 ggtatgcagc ttccccagcc ttgctcccgt tcttttttct tcctttataa gctcgtattt
     4681 tatagtcaac taagtatacc tccattaaaa cagcagaata tatttatcat gattcattgt
     4741 atttcgctgc ccataccctg tgtatgactc catcgttata gctcagcacc tttaactcag
     4801 catctgcaat ccggtaatat gagccttcat ggatatccct gcaggattgc ttggataaac
     4861 ggcttattaa accgcctacg gtatcggcga gatcttcagg tatttttaca cctattgtgt
     4921 tatttatatc agatatactg gcagaagccg ggaaaatatt gatagcttcg ctgtccctta
     4981 aattgcttat aaacagatac ttctttattg caaaaatact ggctatagag actattccga
     5041 gaaaaaattc cagcatatgt gcattgctta tcagcatttt tcttgccaca gcataaagta
     5101 aaacctctat tacactgccg ggtgtgtgcc tgacaagcat ggccaccatc tcaaccccta
     5161 ctacgaggag aagtgtatgc cccagaaact tttgaaacac ctcataagtt tgcaaagtac
     5221 tggtctgaaa tatcaacaca atatactttg ccagatctat cataccgata actatagcta
     5281 atataataac tattgccaat atgttctcca aatacagact cgcattaata atgctgtgtt
     5341 taattcgagt cattttcatt gaaacacctc ccaaaattaa ataataagca tcgcatctcc
     5401 aaagctgaaa aatctgtagt gttccttcac cgcttcttta tatgccctca ttatgttatc
     5461 tctgcctgcc aaagcactta caagcataag caaagtagat tcaggcagat gaaaattagt
     5521 tataaggcca tcagttattt taaactgata cccgggataa atgaaaatgt ccgtccagcc
     5581 tttgtcttcc ttcaggcttc cttttctctg cattatcgat tccagtgtcc ttgcagaggt
     5641 agtgccagta catattattc tgccgccgtt tttccttgta ttgtttataa tctcggcact
     5701 ttcagcagta agctcatagt attccgaatg cattttgtgc tcctcaatat tgctgacctt
     5761 taccggcctg aaggtaccaa tgcccacgtg aagcgtcaaa taagctatat tcacacccgc
     5821 ctctgccagc tgatgcatca gctcatttgt aaaatgcagc ccggcagttg gtgctgcagc
     5881 tgagccttca atctttgaat acaccgtctg ataacgttcc ctgtctgtaa gttctttctt
     5941 aatataaggg ggtaaaggga cctttcccaa gcggtcaagc acttcctcga agatgccttc
     6001 gtagttaaat ttaaccaggc gattgcctcc ttcaagtatt tcaattatct ctccggaaag
     6061 ctctccgtct ccgaatataa attccgcacc tatctttgcc cttttccccg gtcttaccat
     6121 cacttcccag acatatttat ccttccgatt aagcaaaagg aattccatat ttcccgtgct
     6181 gcccctctta acaccaaaaa gccttgcggg tattactctt gtattgttca tcaccaggca
     6241 gtctccctca ttcaggtatt ccaaaatatc tctgaaactg cggtgcacaa tttcaccggt
     6301 gtttttatca agaaccagaa gcctggacat atccctttcg gggatcggct cctgtgcgat
     6361 caattcctca ggaaggtcat agtgaaaatc cttaagttcc ataccgtgct ctctttctat
     6421 tgtacatttg cgttctgata gtagtgttcc aatattttct gatagttata acctgcttca
     6481 gccatacctt ttgcaccgta ttggctcatt cccagaccat gcccgaaacc cttcccgtca
     6541 aaggtatatt tgtcgggaat cacattatag gctttagtat cgttcatccc tttaacgctt
     6601 accttgttcc cctttccttt aacaactgct tttcccgatg cagaaacaac atacatattt
     6661 gaagttcttc ccttttcggc agaatttaaa atggaacctc ttacaaagac atccgcatcc
     6721 gttttcaaat cgtaccatat gctcttaagc agcctcgtac ccaatatgct ccttatcttt
     6781 tccttctcga aaactgttgt atccttggag ccctttacct ccagcttggt aactctcccg
     6841 aacttactgc actccaatat tctgacatct cttatttcac ccaaatctac accggattgg
     6901 gcaagctttt ctttcaattc agccctgttc agttcaaccg tccagttgtc atgggggctt
     6961 ccgaggctga atttatcttc cacccctctg atataatcag tttttgcacc ccaaacattc
     7021 tcactgtctt cagtgtgccc tccgctgctt gagtggtaaa aagcggatat tagcacacca
     7081 ttatatgtaa ttactttgcc cttggtggca ttaacagctt ctgtggaatt actgttttcc
     7141 tgcgccaatc cgttatatgc ctggcagtgc tcagtactgc acaggtcaaa gccatgagca
     7201 ttatgccttc ccatagtcac cagcgcaaag ttccttgccg ccaccgcctg tgccttcaag
     7261 gcttctatat gccaggaggc cggcatctca gaaggaacaa cactatacaa atattgatca
     7321 agggaaagct catttataac tgtgatatca ctttcttcaa aactttgcat tactatgctc
     7381 cctctgtatt tctttccttt atactgcagt gaacttactt ttccattgac ctccttgggt
     7441 gtaactttaa catttccctc actgttcaat aaaagcataa gctttccggt agtattgtca
     7501 ataatctgta ttcttttctt gtcaggatat acaacagaat attttaaatc acttgcttcc
     7561 ttcttcataa tctttatctg ttcaaggcat tcgctttcat caagatacag ctgtgaccac
     7621 accctccatc cctcttcata tgcaagaaat acggactggg taatggatga tacctgtttt
     7681 aagacttgca gggcggcttc tgcattagca tacacccccc ctatttgtat atgatatggg
     7741 ccgactactt caccctcgta tttggctgcc cggacatagt ttatttcaca ttccttgccg
     7801 cctgcaatat tatagtattc atccctgcgg accttcacaa ccttaggatt tttcacttca
     7861 agcaaattct tgtatgagcc cttctcaaga ataagaatat tcatccctgt ctcacttgca
     7921 agagtaaaaa tatttgcctg ggattgtcca aagctcaggc cgattctgat tgtctcagga
     7981 actgcaatat cggcatgcaa aatcataaac ataactgaag taattaccaa atagagtact
     8041 gcaaattttt ttgacttcat aagcacaaac tccttttatc acgcattcct tgcactatct
     8101 gcgaataaaa ccgaatacta tcgtcattat aatacttgca agaatacagg ttactatcgg
     8161 aaaataaaat gtaaaatttc ccttttttat ataaatatct cccggaagcc tcccaaatcc
     8221 cagtttgccg ccaaagtaca gcagtgtccc gaagataacc attccgattc caagtactat
     8281 tattattttg gcaaaatatt gaaagttcat tttcgaccga cctttctccc gggcatgtat
     8341 ctgtcccttt cgctgtaaat acagccgcag tatggctgcc tgtacaagcc cagttccctg
     8401 gatttttcaa caccctcctt gaagcctccc ctgaaatcct tatataggaa ttccactccg
     8461 tatttcttcc ctgcttcctc tcctatcctc ctgataaggt catgcttctg gaaagggctt
     8521 accagaagtg tagtggtaaa agcatcaaaa cagcctgatt ttgcaacaga agcggctctc
     8581 tccaaccttt cggcataaca cataatgcat ctctgccctt cacggaaagc tgcattcctg
     8641 agaaattcat ccaaattata ttcatcaatg gtaatcagct tgtaacctga tttgtccgca
     8701 aaatcccggg ctgtgtccag ccttttctta tactccgtat aaggatgtat attggggtta
     8761 tagaataacc ctgttatttc atgctcccgg ctcagaacct gatggggata aacggcacat
     8821 gggccgcagc acatatgcag cagcagcttc aatactctca ctcctgatta atcttttttt
     8881 attccaaaat gcctgtatgc ggaatttgtt gcaactcttc cccttggtgt cctgttaata
     8941 aatccgattt gaagaagata cggctcgtaa acatcctcaa tagtaccgct gtcttctcct
     9001 atggatgccg caagagtatc caaacctacc ggcccgccgt caaatttatt gataatggta
     9061 agcatcatct ttttatcaat gtcgtcaagc cccatgtcgt ctatttcaag aagcttcaat
     9121 gccctgtcgg ctgtttcctt gtcaatgact ccggaagctt tgacctgggc aaaatccctt
     9181 accctcttca gcaatctgtt ggcaactctc ggagtacctc tggatcttct tgctatctca
     9241 aaggctccgg cttcatccgt atctatcttc agtatccttg cagaccgctt gattatcttg
     9301 aaaagctctt catcagtata atattccagc ctgcagatta ctccaaacct gtctctcaga
     9361 ggtgcagtaa ccataccggc tcttgtggtg gcaccgatca tagtaaatct aggcaggtca
     9421 agcctgattg agcgggcact gggccccttt cctattatta tatccaaagc ataatcttcc
     9481 atggcaggat acagtatctc ttccacactt ctgttcagcc tgtgtatttc atcgataaaa
     9541 agtacatcat tattatcaag attggtaaga attgccgcca ggtctccggg cttctctatg
     9601 gcaggcccgg aagtgattct tatgcttacg cccatttcat tggaaataat attggcaaga
     9661 gttgttttcc caagtcccgg aggcccgtaa agcaggacat ggtccaaggt ttccttcctc
     9721 ttattcgctg cttgtatgaa aatgctgatt ttttccttgg caacggactg tcctatgtat
     9781 tcctcaaggg atttgggcct caggctggta tcaatctcta tatcatcttc tattatcttt
     9841 ggggttatca atctgtcttc cattgtattt cctcactttt atcagttcaa tattacttca
     9901 tggtcccaag ctgcctcaat gcttccttaa tcaagatatc gataggtttg tttgtatcct
     9961 ttagcttcat caccgcattt gccgctatag aataattata tcctaaagcg gtcaaagctt
    10021 caattacctg ggatatctcg tcgctcccgc cgaaagcccc gattgctccc actgcgcttt
    10081 ccgtatctat tttgtctttc agttcaagta ttatcctttc tgaaatcttc tttccgatac
    10141 cctgcgcttt tgaaagcttt gctacgtccc tgctcaatat ggctgctgcc agctccgatg
    10201 ccttatattg attcaatagg gaagctgcgg cctttggccc tactccggat accgatataa
    10261 gcagtttgaa cattccaagc tcttcccttg tagcaaatcc atatagtatc aggtcatcct
    10321 ccttgactat caagtgtgta aaaatctttg aaatctcacc cagcttaagg cttttcattg
    10381 aattctgcga agtaaacacc ctgtagccca ggcctgaaat gtcaattacg gcataatcct
    10441 ctccgatata gtccactctt ccgcttaaat actcatacat tgtacttcac ccctacactc
    10501 tgaattgttc cgagaatttt gacgaattgc cgtggcaaat tgcaacagcc aaagcatctg
    10561 cgacatcatc aggcttcggt atctcaccaa gattcaatat taccttcacc atctgctgca
    10621 tttggttctt gtcagccctt ccataaccta ctacagcctg tttgacctga agaggggtat
    10681 actcatatac ctgcacgccg gattttgagc cggccaggac agctactccc cttgcatgtc
    10741 cgactgtgag agctgtcttg atatttttgt tgaaaaacag ctcttcaatg gcaaaggcat
    10801 ccgggttatg cctttcaata atatcaacaa gcctgtcata aattatggta agccttgcag
    10861 acatatttat gcctgaatct gtggtaatcg ctccatagtc aataaccgtg aatctgtttg
    10921 cttcatattt tacaagtcca taacccagta ttgcaatacc cgggtctacg ccaagtatca
    10981 acaatagata tacaccacca aacatatatt cgctatattt actataactt ttttggggaa
    11041 gaaatgcaac agggcagcag gattgctgcc ctcaaattgc tgacaaagct caaaatagga
    11101 acagtaatgt atagtcagag cataattata cagaaattgt taatggctct tatgaggctc
    11161 gacttagaaa caaccgtatg cgaggcatgg aggccgagca agctgtctcg gtcacggatg
    11221 acctttgaca gcgttaggtt tttcttagtt gagccgaatt agagccatca atttttttga
    11281 gacacgggga cgtttcgcgt gtctcacttc agcgagacag gagaaacgtc cccgtgtctc
    11341 atttgctgtt cttatgttga aggtttttca tcagcctcag ggcaatagaa ttgctgcctt
    11401 gaattaacgg acataagaag aatgggttgc gttatttaat tgctgaagcc ctccagggtc
    11461 tgctccttct gctcctctgt atctaccact atcccccccg caagggcctg ctgatcctca
    11521 ggagtaagcg gagctgcgga aatgtctgtt ttttcctgca gggacttatg cccgttctca
    11581 tcatatacat aaatcgcaat atacccgccg tccactccta ttatataatg gccggggcag
    11641 taagtatcaa tattcctgct gaactcaact acaggaggtg aataacctga aaggtgccaa
    11701 tggctgtatt tttctttcag ctgctgctca ttcatattta tctcgtcgct tcctgcctga
    11761 acagcttttt ctacggtatg cctgcaggaa ctgtagtatg tcttgaaaat caactgagta
    11821 ttcggtgtgg gaaaatctat ctctaaaata ttcgctgtct cctctgtatt gctttcggca
    11881 ttaacctctt catagggctt tctcactgtt gtcggattaa tcaggctgtc aggtatctga
    11941 aggttggcag atggctgatt atcaggatta tctaaagctt cagggatatc agcattgaaa
    12001 aaatacccga ccgcaaaggc ggcggaaatc agcagtagaa aggccgcagc cagcagatac
    12061 cttcttttgc gtctgatgct tctatcttca aacataatta tccctccata ttgttctgga
    12121 gggtattatt tccaaaatat atatttttat acctatttat agaatattct tcttttacct
    12181 gcctgttagc tatatcattt tcaaacttgt catgttgtca tagtatacgg tatacgtatc
    12241 agatttgtgt tatttcagat actattcgtt attaataaat gaaaatgagg gttgatacta
    12301 tgacaggatt gggcttcgcg tgtatctcaa agagcttgaa ggaaaacggc agattcagga
    12361 caatgagcgt aaaaacctat aatagcctga aacctgatga aaggattcga aggcttagat
    12421 ccatatcagt ggataacctg tacaacacct gcaggattat actttggtgc attgaaaaca
    12481 gtataagcct gtatcggtta agctcgggcc ttatacccct ggcaggctgc gtaccggaat
    12541 ggaaatggtg ggaagaccgg gatataagga acatgtgtga aaagataaaa aagattgttg
    12601 atgaaggcaa aataagaatc agcatgcatc ccgaccaatt ctgcgtgctt aactcagaca
    12661 aggctgaggt agtaaaggga agcattgcaa ttcttgaaca ccataacaga ctgtcaaata
    12721 tggtgggaaa caggattcta gtaattcacg caggcagcgg caccggaggc aaaagcaaag
    12781 caattgaaag gttcatcaag aattttaaca tgctggatga agatattaag aaaaaaattg
    12841 caatagaaaa cgatgacaag gttttcaatg cggaagacat tctgcacctg tgtgagcatc
    12901 tgggagtacc gatggtgctg gatatccatc atcataactg caatcatgaa aaagacaacc
    12961 tgccccagct gatggatcgt ataaaaagca cctggaaggg aaaaaggcca aaggtgcacc
    13021 ttagcagcgg aaaaacctgc cctaccgaca ggcaccatgc agattatata gattttggcg
    13081 attacaggca ggcagcagaa ctcgttaaag atgaatttga tataatgatg gaatgcaagg
    13141 aaaaagacct ggcagtactt aagattctca catattgcat ctgatttcaa gatatactgt
    13201 acaatcatcc ttgaatataa atttattgtt aaagcaaaat gtttaaaacg atgaaggtat
    13261 tataggagga tgtttgacgt gttcataaat ggaaaaatcc cgcacctttt gcagcagact
    13321 gtgctcatga ctgcttttac attattgaag ttcactgcct tgtaccactt tgccggaaag
    13381 cctcaggata tgcttcctgc agctttaagg acatatacgc tcctgtttgc catatattta
    13441 ttgaccaacc tggtcatagg aagaaaaagc ttcttaatca gaatagcctt tcatgaattt
    13501 ctggctctca ttgtattggc agatacgctt tattacaaac aattcaatat actgccggaa
    13561 gttgcggatc tgcagtttct gaaagtcctg ccgacaatat gggacagtgt aggctttctt
    13621 ttttcaggga tacaccttct gcttttcgcc gatgccctga ttttgatgac tcaccaatat
    13681 gccctgaaca gacaggaaaa ggcttcagcc cctgggatac tgccctctgt tatcgcagct
    13741 gcgcttttgc ttgcaattat atttacagac ttctctttca gcgatataaa aagcagcggc
    13801 caggcctatg aaaaattcgg cctgctgcat tatcatattt cacaaattcc gggcgtattg
    13861 cagtccaaca gcaacagcag cagccggagt atcggcaagg ctcagattcc ggcagaggtt
    13921 gaggaagctg ctaaagagcc caggtgcttt ggaattgcaa agggcagaaa cgtaattgta
    13981 atacaggtgg agtcccttca ggattttgtc ataaatatgc attatgaagg tcaggagctt
    14041 acgccgaatc tgaacaagct gcttctgaaa gatacaatat acttcaaccg tttctaccag
    14101 cagctgggga aaggcaatac ttcggatgcc gaatttgtca cccagaattc cctatatcct
    14161 tcgattgata tacctgcata cagaaaatat gagaataaca gatttatggg gctccctatg
    14221 attatgagag aaacaggcta cggcacctac gcctttcatg catataagcc ggacttctgg
    14281 aacaggcaga atatgtatcc catgcttggt tttgaccgtt ttataaacat gaacgacttt
    14341 aaccttaacg agacatttgg tatgggctta agcgacaagg aattttttga gcaatcggcc
    14401 gcatatatca gtactgccaa tcagcctttt tactctttcc ttgttacttt atccagccat
    14461 caccctttcg gcctaccgca aaaatacaag aaaatacagc tgcagccggc acataaggat
    14521 actctgttcg ggcgctatat tcaggttatt aactacactg atgaggctat cgggcttttt
    14581 ctcgaaaaac tgaaagaata cggcttatat gacaactcaa taatagcatt atatggagac
    14641 catcgtagca ttcccaacgg cgtccctgaa aacgacaagc tgatgtcgga gctcttggac
    14701 catgaataca cttttgatga gtctctcaat gtgccgctta taatacatat tccgggaacc
    14761 aatataatag aaaccaatac aatagtcgga ggtcaaatgg actttctgcc cactatgctg
    14821 aatctgctgg gaataaaaga aatccggcct aagcttttcg gacaggattt gctaaatgcc
    14881 aaaaacggat ttgtggcatc tcaaaccggt atgatcaagg gttcctttat agacaatgag
    14941 aaaatatttg tgatgtcaag agacggtgta tttgagaaca gtaccgcctg gaagctggat
    15001 acccgtgaac ctgttgaccc ggaattctgc agaaatggct atgaaagggc actgagagag
    15061 ataaaaaaat ctgaatatat tctggcaaat gatcttattc atgagtttat cacattgggt
    15121 aaaagccata caactttcag cagtgctata gaggccgctt cgggctatat agaaaggatt
    15181 gccgcacttt tcaagagtat tgttgaatag aagcagaatg cgacgcggac atggggggga
    15241 cacggggacg tttctccccc atgagcaaaa ggaataattt acttgaagca agcattgtca
    15301 aagcatgata gaatttactt gagtataccc atattcagct tatcaaaaaa atcggaggat
    15361 tattattatg aagaaatcta ttgtcctgct ttctatcctg gccttgctgg ctgccgtgtt
    15421 tgcagctgtt gcggcaaagc ctttgaaaat aggcgatgtt attgggaaaa tctattatac
    15481 tgatataaaa acctatattg acggaaaact cattgaatcg gtaaatattg gcgggaggac
    15541 aatgatatcg gcagaggatt tgcagtatat gggctatctt gtagaatgga acgatactgc
    15601 aagaacactg aatattacat caaacacatc ccataatatt tcgggaaagg tcataatacc
    15661 tgacgggaaa gtcgccccca agggtggttt gaatgttgaa atcgctgcct ttatcaatcc
    15721 aaaccctgac ccttcggtta taagccgtgg ttatggccct gagttcggca aggaaattag
    15781 acttgtcatt cctgaaggcc atagcagcat agaatatata atatccgtac catataaatt
    15841 cgaaccgcgt tatataactg caatacttcc cggcggtgaa tcgggagaac ctgaaaagtt
    15901 gcctgatatt aagccatact atgtactccg cttcagatac tcatattatc tgggaaatca
    15961 taaagttgaa cacggagatt tcatggaagg ctatcttgat atgagcaccg gcgataaaac
    16021 aggcgttgac tatacctttg acaatattga tttgaaataa actatgcaag ctgagctgtc
    16081 tgttatatct tcggttttct tgttactgtt aatgattttt cccaaacttc taaggctcga
    16141 aacaaatgta tcggcttttt ctgccggtat cattacagtg atctggcctg aggattcgtt
    16201 ttgataggac tcacgggtgt atccccctga ggccgctgca gcctgctgac ataatactta
    16261 tgatatgttt tacattatct tcataaatat tgaaatgcct gaaaagaaca ctccggacaa
    16321 taaaaaagat agccaggcaa atggctatcc gctctataag tcaacccttt actgctatac
    16381 cgcatcttct acaggcagct cggcgttgtg gtagacgtcc tggacatcat cgttgtcatc
    16441 gaacatatcc agcatcttgt ttattttctt tactgcttca gggtctgtaa ctt
//
LOCUS       DAOQUR010000048        16136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4110_length_16136_cov_9.585225, whole genome shotgun sequence.
ACCESSION   DAOQUR010000048 DAOQUR010000000
VERSION     DAOQUR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16136
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4110_length_16136_cov_9.585225"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1539
                     /locus_tag="PKI50_09465"
     CDS             163..1539
                     /locus_tag="PKI50_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT03968.1"
                     /translation="MEYVNPLGKEKVSKLLAEYSVPAIVGMIVNSIYNIVDRIFIGNS
                     SDLGTFGLAGITIGFPIMIILLSMGLLFGVGGATLFSIKLGEGQSEEADRALGNAFSL
                     MVIAGALFMITGQILLRPLLTLFGASEAVLPYSMEYMRVIFFGAIFQVIGMGMNNFIR
                     ADGSPRTAMLTMFLGAGLNTLLDPLFIYVFKMGMTGAALATILAQCISATWVVMYFIG
                     KHSKHKLKLKYMKLKLNVVLTITSLGIPGFALQLANSLLNAILNKSLLIYGGDIAVSG
                     MGIINSLQTIFYMPIIGIKQGVQPIISFNYGARKFDRVKEAVKLAVISATAIVVFGYV
                     ITRLFPRQIISIFNRDPDLIEFGSYGITAWFLCMPVVGFQIITSHFFQAIGKSKAAMF
                     LTLTRQVIFLIPALIIFPKFWGISGLLHAAPFADFFSALLTGIWFYYGNKNLEKYPLA
                     TISNNSIK"
     gene            1721..1939
                     /locus_tag="PKI50_09470"
     CDS             1721..1939
                     /locus_tag="PKI50_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03969.1"
                     /translation="MRNLSDAEVLSLTALFKMEQDSLTVSRALHALISNDELKKQSEA
                     GMLAAEGRVKGIRQFIVENGITNIQEVQ"
     gene            1941..2234
                     /locus_tag="PKI50_09475"
     CDS             1941..2234
                     /locus_tag="PKI50_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HNT03970.1"
                     /translation="MASMIGNIVKNNTDITDEVILTSMLGSLKAIAAAYFTATMTSTT
                     PELKSMFSSALVQVMNSHSVLTELAVKHGWEKPYVSPAEQLADVYKYSITEKQ"
     gene            complement(2345..2530)
                     /locus_tag="PKI50_09480"
     CDS             complement(2345..2530)
                     /locus_tag="PKI50_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03971.1"
                     /translation="MGEFKVVCCQCKSDNILEKYSRNRLEWVGGIIKYGEGIQRKCCE
                     CDNESFMIFRTWEEKSP"
     gene            complement(2533..3633)
                     /locus_tag="PKI50_09485"
     CDS             complement(2533..3633)
                     /locus_tag="PKI50_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNT03972.1"
                     /translation="MKVAIIGAGTSGLSCAIELERNGIYPVIFERNEFIGEYHPHVSA
                     LLGLITRPASDPVKYLDKVLGIKLKPLSQFRKVIHYSPNNQAAVTGPLGHFLIRGKEE
                     NSVKNQLRSQVKSQIIFNSFVQPEDLENEFDYIVVADGHWSVPARYGIWQEVMRTWVM
                     GGIFEGDFEEDTLQMWLDNELTKGVYIYSAPYSKNKGVIAHVVQSIGQEELNGYWHRF
                     LQSRDILKKYDMLETWKLPHRAGSVTTNRVGKIYFAGVAGGGIEPFLGFGQFNAVVSG
                     VMIARSIARGIDVNLLLKDIHKKSRELSALRPMLNAADNKDFDRMMTIMKAPGLRSLV
                     YRTNIDVIKMLSTGLRLAASEKKIASQIKGGK"
     gene            3861..5186
                     /locus_tag="PKI50_09490"
     CDS             3861..5186
                     /locus_tag="PKI50_09490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT03973.1"
                     /translation="MKKISGKKSRLYIPVSIKFWLSHLFAILWTSLSIYISLPWLEDF
                     SGVFTFPAAFIIISGIAYFPGYLNAFLFASLMLDRQPPFKNEYPARDITVLIAARNEG
                     ERIADTIYYIAKQDYTGNIKVLIIDNGSEDDTAVNALKAGEAHNLDLSVIREEIPGKF
                     NALNAGLEHTSTDLIITLDADTLLHRSAIRYLAARMESAPKEVCAVAGSVLARNSRNA
                     LLAKIQEWDYFLGIASIKRLQGLYQGTLVAQGAYSIYKAESIREVGGWPDAIGEDIVL
                     TWNLLKKSGPIYFEPLAVAFTDVPTTIRSFSKQRSRWARGMIEALKQVKPWQQPLPYT
                     KYLTGMNLIMPFLDFCYTFFWLPGLILAFFGYYWIVGPMTLLVIPLTLMNYSILYAYQ
                     LYVFRRLNLRVRKNMRGFILFIIVYQMIMSPVSVWGYLQEFLKLKRNWK"
     gene            5297..6790
                     /locus_tag="PKI50_09495"
     CDS             5297..6790
                     /locus_tag="PKI50_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HNT03974.1"
                     /translation="MPAINEEDSLYNKTADVSSDNIRSILGSNADFVSRVILVRNKPE
                     IPSVLVYMDGLVDISLINDNILKPLSQEEQFDSCSGEEDAARLISMGKIYSSSMKVRT
                     NMKDLTDDILTGSAALIFNDIRTAFTFEAKASLSRTISEPTGETVIKGAKDSFIEDLR
                     TNTALCRKKLTSPYLTIEQIRLGKQTKSNIAILYVKNIAKSKLIDEVKQRLESIDIDR
                     VLDAGVIEGAIVDDKYSAFPQIQYTERIDRLCASLVEGRAGILIEGLPTAYIVPGTLL
                     QFLQAPDDYSFQFIIASIVRFIRFMSMFITLVLPGFYISITSFHPEMLPAELAFSIVA
                     SRVGVPFPMFVEVLILLLAFELLIEAGVRLPNVIGQTVSIVGGLVVGQAAVEAKLVSP
                     ATVMIIAITAIAGFLMPNQDFSNAIRLWRFILAIFGSITGIFGLTFGLIFLLYHWCRI
                     ETFGIPYLDPFVANEDEQLQDTIFRLPLFTMKKRPSSLDPKNKKRME"
     gene            6798..7985
                     /locus_tag="PKI50_09500"
     CDS             6798..7985
                     /locus_tag="PKI50_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HNT03975.1"
                     /translation="MRKYAILTVLLALTAALSGCMQIIMGRSEIDKLFIIRMFSFDEA
                     EEGKIKVTATTKDLSTGEGGGEVLQKGESIAAEGETVFDAVRNMSTYADRKLNAGHTE
                     FILFGESLARKGILPYLDLISRHPEFRYNAKLYVVKGGTANSLAEKASTSKYFVGDRL
                     AHIEENIGRTSISGTVTLNEVLLIFDERYLDTFIPYIEPVPTKATGEKPDTCDMLLNG
                     YAIFREDKLLYFASREESRGINWIMNRIGSGIIVLKDQTGQEISMIIVKGASRVIPEI
                     VGNELRCRVEISFSTNIGEIMGGRSVFDKASLDYITEQQENSIKREVENAIRIAQDKN
                     SDHFSIISKFRIKYPLLRDYFQQNWQELFPDIKFEVSVDSLIDGTYMINEPVTASKEA
                     VGE"
     gene            7982..8185
                     /locus_tag="PKI50_09505"
     CDS             7982..8185
                     /locus_tag="PKI50_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03976.1"
                     /translation="MKIFLMVVIVVIAITAEMIPVIKKKQHKEAAVLIMLGAVTLAYG
                     YYYITHVHTASLANLMFDIFRLR"
     gene            8198..9298
                     /locus_tag="PKI50_09510"
     CDS             8198..9298
                     /locus_tag="PKI50_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HNT03977.1"
                     /translation="MKTGKDKISVKQLFFVFTIMVSSPTTRLLPKYAAAKAEQAGWVS
                     PIISTVPFILLILAIDSLLKKYKGQSMSDIISNILGRFLGKLVLVVYLLWALWLAAMY
                     TRYYSERLTSSIYPNISVGLFIILALIPIAYILRSGFTPIARMSEILLPFIGAMLIML
                     ALFLFPKVRADNLLPVYFNDIIPIFRGSIAVTGILSYLFLMFFLSDNVVNLKSLRVFG
                     YIAAYVNISSIMLVNFIVIGVMSSSLARRVSVPVLTVVKQITIMDIIENIEAVVVAIW
                     IFADFTMITTLLVIVLNLIKSIFNLSDTRLLINMLAVLVYFLSMGVCARRFELEDFTN
                     SLLMPINITLGYVFPFVLFALSKLKKTTNPSR"
     gene            complement(9336..9800)
                     /locus_tag="PKI50_09515"
     CDS             complement(9336..9800)
                     /locus_tag="PKI50_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HNT03978.1"
                     /translation="MNNLTQKEKGLLQDQKTHEEICIKKYNEYAGKAQDPQLKQLFSS
                     HAKHEQQHLNTINQLLSGQMPGMNQNQQQGRQGAQGQQPIRTGTGSKSDGDLCNDILM
                     TEKYVSGAYDTAIFEFRDPNIRQTLNHIQKEEQQHGESVFKYMESMGLYNPQ"
     gene            complement(9846..>11861)
                     /locus_tag="PKI50_09520"
     CDS             complement(9846..>11861)
                     /locus_tag="PKI50_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HNT03979.1"
                     /translation="EDVEAINCKKVQVEFSEAIREPGSYKITYEDDDGKLRTIDIDFV
                     EVDPEDNTRVNIYTEDKMSDEYYYILEPKSAAADIAGNKLDIDDLEIDFEGSSIMSSD
                     YIQGVDLLNEYNLRVYKSSRISRVSSLYRLDGDGNAVGENLIDSTSRISSNVFRVTSK
                     KPLYKNVKYRITADGISYSFRGIADDDNKPEPPLKSSSKEITSFGFNNIAGGIDGNID
                     KDAKRISLTVPHGTDVKKLAASFKCSEHAVVEVKGKRQISGETTNDFSGEVTYTVIAQ
                     DGSSQDYKVVVAIAKELDKSIKAFRFEKPKSAIGEIDEGKRIITVTVPYRTDLSKLVP
                     VIDIPDNTRISVKTGKEPDFSEPVEYIVTAEDGTTKEYTVIVKKAQNTENRITVFAFR
                     ALEPVIRAEVGKEQKTIELTVPYGTDRTKLVASFKASPEAVVKVKGEEQVSGETVQDF
                     TNPVKYIVTAQDGTAREYTVIVNAAPSSEKLIKEFGFSVPEAEGVINEAESKINVTVP
                     FGTSLNALAAVFVSSENSKVLIGSDEQKSGETVKDFTNPVIYTVAAQDGSTRNYTVTV
                     RAADENEKILSKFEFAGLNPKARGIINQQNRIIRVSVPSDTIVSNLIASFSYLGKSVY
                     VGEVQQYSGITPNDFSRKVVYRITADDGSYIEYKVIVTVMEGEIKGF"
     assembly_gap    11862..11961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11962..14684)
                     /locus_tag="PKI50_09525"
     CDS             complement(<11962..14684)
                     /locus_tag="PKI50_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT03980.1"
                     /translation="MVKKFVTVITIFALVLSAILPVQVIADDDIGYEGRICRDLGILK
                     GHTGIVDEEYLETRPSRLQAAIMFLRLKGLEQDALDYYGGKNFKDAGVVAWEEGRNVL
                     SYLKNHPELGWIGDGVNFMPYSLIDSRAYYKVLLESLGYKQKIDGKGDFDWDEVLDFA
                     EEKGLYKVAGIRNFTVKSLAIATVEALNTKMKGERRTLIEYLVDIGDVDKWDAISAGL
                     YSKELEAEVKDVRAISNSKVEVVFKESIDGTDAEDEDLYEIKHLDINKVSMKNEYAVI
                     IDTSAMKENTSYTLEFNDKDYSFRGLKKDKTSPKLIKIECRDTDLLELTFDRVLDNET
                     AQDTDTYTISGASVRSAELDSTNTKVRLTTSGIESGKSYELKIRNIKNGDGAVTKSIT
                     KRFTGRRDTSPPKLSKLTVLNNVRLLLEFSDSNGLDKSTAQDKNNYSITGGDGDLEVE
                     TAEVKDMDEDGLWESVYLVTESQEAGTKYTLIIEDMMDDSVLGNNITRIIKKEFRGKS
                     KDKSGPTVANDPRAVTNTMVEVEFSDSNALDVESACDIDCYEIDEGLEILDARIKNPD
                     DLYSVGGRTVLLITEEMEKNERYTLRISGIYDEFGNEMKSSSSDKKYRFKGIGEDRTP
                     PYITSVQCIDSRTLELHFDDTLDEGSAENIVNYRIDGLAIVTKAVLQEDGKTVRLTVS
                     SLSSDRNHTVMMNNIKDLSGNRLSNVSVDILYNGSVYDDDPPEVDYIEAVNRKEVWIH
                     FEEEVYAENAGMEASGIDFKQAGSVLDDGTVVVMVIDGNESLKDDREYEVTKLTGIWD
                     MRGNKYEFEYGLEFDGTDDENDPPEVDDWDQMDVRRFRVVFTEPVLLKGDGVSGIKNP
                     GGVGIEWKAVLNPDDGDDDEAYCTVDYIAKNKNIPPDKEFRFNFTEMVEDYA"
     regulatory      15138..15239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            15300..15815
                     /locus_tag="PKI50_09530"
     CDS             15300..15815
                     /locus_tag="PKI50_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HNT03981.1"
                     /translation="MSKAKTIAFVGLLVSMEIIFTRFLSIQTPIIRIGFGFIPIAFTA
                     ILFGPLIGGTAAAIADITGMMIFPKFPFFPGFTLSAFLTGAIYGLFLYRKPITIVNIG
                     KSVLLITILVDLGLTSLWVYMTTGNAASVFLMPRILKSAIMLPIQIVTINVLWKYIGS
                     HIDKARYAKEH"
BASE COUNT         4236 a         3709 c         3210 g         4881 t
ORIGIN      
        1 ggacggaaga ataaccccgg actgatttaa tctcctttaa ttattttccg tttaataatc
       61 ttgtaaaact attcttctga attgaataac ctttacatta tgatataata tctatgtatt
      121 caacaaatat cttgtttata atatgcaatg gagaaaacta acatggaata tgtaaatccg
      181 ttaggtaaag aaaaagtttc aaagctattg gccgaatatt cggtacctgc cattgtaggc
      241 atgatagtta attccatata taatatcgtc gatagaattt ttataggaaa cagcagtgat
      301 ttaggaacct tcggcttagc cggaatcact atcggtttcc cgattatgat aatactcctc
      361 tcaatggggc ttctgttcgg ggtaggagga gccaccttat tctcaataaa attgggagaa
      421 ggacagtccg aagaagctga ccgtgccctg ggcaatgctt tttccttaat ggttattgcc
      481 ggagcattat tcatgattac aggtcagatt ttacttcggc ctttattaac cttgtttggt
      541 gccagtgaag ctgtattacc ttactcaatg gaatacatga gggtaatttt ctttggggcg
      601 atttttcagg taataggtat gggcatgaat aattttatac gcgctgacgg aagcccccgg
      661 acagcgatgt taacaatgtt ccttggcgcg ggtcttaaca ctttgctgga tccattattc
      721 atttatgtct ttaaaatggg tatgaccgga gctgcacttg ccaccatact ggctcaatgt
      781 atttcagcta cttgggtagt tatgtatttt atcggcaaac acagtaaaca taagcttaaa
      841 ctcaaataca tgaagcttaa gctaaatgtt gtattaacaa ttacttcttt aggcattccc
      901 ggatttgcat tacaactggc caatagcctt ctgaatgcaa tacttaacaa aagcctgctc
      961 atatacggag gagatattgc agtgtccggc atgggaataa taaacagtct tcagactata
     1021 ttttacatgc ccattattgg cataaaacaa ggcgttcagc caattatcag cttcaactac
     1081 ggggcaagaa aatttgacag ggtaaaagaa gcggtgaagc ttgctgtaat ttccgcaact
     1141 gcaattgtgg ttttcggcta tgttataaca aggttgttcc cgagacaaat catatccata
     1201 ttcaatcgtg atcctgatct gatagagttt ggaagctacg gtataacagc ctggttctta
     1261 tgtatgcctg tcgtcggatt ccagataatt acttcacatt tctttcaggc tatcggaaaa
     1321 tcaaaggctg caatgttctt aacgcttaca aggcaggtaa tctttctgat tcccgccctt
     1381 ataatattcc ctaaattctg gggcattagc ggtttattgc atgctgctcc ctttgcagac
     1441 tttttttcag ctttattgac cggcatatgg ttttattatg gtaataaaaa tttagaaaaa
     1501 tatccattag caacaatctc aaataactcg attaaatgat cattccgaac acatcatcag
     1561 aacttttcaa aaatactcgc atcaatacat ggtaaattca tggtaaattc catttttact
     1621 tgcctttctt gcctatcttg cgccctcttg tcccctgaat agaactgaca aatttccggc
     1681 agaataataa ataagatcaa ttttaaggat ggtggcatat atgagaaatc tatcagacgc
     1741 tgaagtacta tctctgacag cactgttcaa aatggaacag gacagcctga cagtatccag
     1801 ggctttgcat gctttaatca gcaatgatga attaaaaaag caaagtgaag caggcatgct
     1861 ggcggcagaa ggaagggtca agggcattcg gcagtttatt gtggaaaatg gcataactaa
     1921 tatacaggag gtacagtaat atggcttcaa tgattggaaa tattgtaaag aacaatacgg
     1981 acataactga tgaagttatt ctgactagta tgctcggctc tttaaaagca attgccgctg
     2041 catattttac tgcaacaatg acaagtacca caccggagct gaagtcaatg ttttcaagcg
     2101 ctctggtaca agtaatgaat agccattccg tactgacaga gctggctgta aaacatggtt
     2161 gggaaaagcc ctacgtttca cctgcggaac agctggcaga cgtatataaa tattcaataa
     2221 cagaaaagca ataacccttg aggatacggg gacgtttcgg gacacgggga cgtttctcct
     2281 gtcccacttt cagtgggaca ggagaaacgt ccccgtgtcc cgaaacgtcc ccgtgtcctc
     2341 ttttttaagg gctcttctcc tcccacgttc tgaaaatcat aaaagattca ttatcacact
     2401 cgcagcactt ccgctgaatc ccttctccgt attttattat acctcctacc cactccagcc
     2461 tgttcctgct gtatttctca agaatattat ccgacttgca ttgacagcag actaccttga
     2521 attcacccaa gttcatttac ctcctttaat ctgcgaagca atttttttct cagaagctgc
     2581 cagcctaaga ccggtcgaaa gcatttttat gacgtcaata ttcgttctgt atactaacga
     2641 cctgagcccc ggagccttca taattgtcat catcctgtca aaatccttgt tgtcggcggc
     2701 gtttaacatc ggtctcaggg cgctgagttc ccggctcttt ttgtgaatat ccttcaacag
     2761 caggttaacg tcaatacctc ttgcaatact tctggctatc attacgccgg atactacggc
     2821 attgaactgt ccaaagccaa ggaacggctc gattccgccc cctgcaacac ctgcaaaata
     2881 gatcttcccc accctgttgg ttgtaacact tcctgcacgg tgaggcaatt tccatgtttc
     2941 cagcatatca tattttttta atatatcccg ggactgaaga aaacggtgcc aatagccatt
     3001 caattcctcc tgtccgatac tttgaaccac atgagctata acccccttgt ttttactgta
     3061 gggagccgag tatatgtata ccccttttgt cagctcatta tccagccaca tctgcagggt
     3121 atcctcctca aaatcaccct caaatatccc tcccattacc catgtcctca taacctcctg
     3181 ccaaatgccg taccgggcag gtactgacca atgtccgtcc gccactacga tataatcaaa
     3241 ttcgttttcc agatcttccg gctgcacaaa actgttgaaa ataatctgtg acttaacttg
     3301 tgaacggagc tggtttttta ctgagttctc ttctttcccg cgaatcagaa aatgacccaa
     3361 agggccggta acggctgcct gattgttggg cgagtagtga atgacttttc tgaattggct
     3421 caaaggcttc agtttgattc ccagtacctt gtccaaatac ttaaccggat ctgaggccgg
     3481 acgggttata agccccaaaa gggccgatac atgaggatga tactccccaa tgaactcatt
     3541 tcgctcaaaa ataacaggat atattccgtt cctctccaat tcaattgcac aggacaatcc
     3601 cgaggttcct gcccctatta tagcaacttt cacatacagc accctcatgc gctaatactt
     3661 acagtgttat tatgcataaa agcagaacta tcattcatcg aaagaaattt tgcctcgttt
     3721 ttcttcattt tcttttaatg tcattgttta cataattaat gcattcgagg tttttattac
     3781 aagtgtatta catcctctca gatgctataa ttactatgtc ataaaattat aaagtcttca
     3841 tttgaaggag ctgattcgga atgaaaaaaa tcagcggtaa aaaatcaaga ctttatattc
     3901 cggtatctat aaaattttgg cttagccatt tattcgccat actatggaca agcctttcaa
     3961 tttatatatc tcttccctgg cttgaagact tctcgggggt ttttaccttc cctgcggcat
     4021 ttattatcat ttcgggaata gcatactttc cgggctacct caatgccttc ctgttcgcaa
     4081 gcctgatgct tgaccggcag ccgcccttta aaaatgaata ccctgcaagg gatattactg
     4141 ttcttattgc tgcaaggaat gaaggcgagc gaatagcgga cacaatttat tatatagcaa
     4201 agcaggatta taccggaaat attaaggtct tgataatcga taacggttcg gaggacgata
     4261 ccgctgttaa tgcccttaag gcaggcgaag cacataatct ggatctatca gttatcaggg
     4321 aagaaattcc cggaaaattc aatgccctga atgccgggct tgagcatacc tcaaccgatt
     4381 taataattac attggatgca gacactcttc tgcataggtc tgccataaga tacctggcag
     4441 caagaatgga aagtgcccca aaggaggtct gcgcggttgc cggatcagta ctggccagga
     4501 acagcaggaa tgctcttctt gcaaagatcc aggaatggga ctattttctt ggtattgcat
     4561 caatcaaaag actgcaggga ctatatcagg gaactctggt tgctcaaggt gcctacagca
     4621 tttataaagc tgaaagcatc agggaggtag ggggatggcc tgatgcaata ggtgaggata
     4681 tcgtactgac atggaatctg ctaaaaaaaa gcgggcccat atactttgaa cctctggcag
     4741 tggcgtttac cgatgtgccg acgacaattc gctccttttc aaagcagcgc tccagatggg
     4801 caaggggaat gattgaggct ctaaagcagg tgaaaccttg gcagcagccg ctgccttaca
     4861 ccaaatacct gactggtatg aatcttatta tgcctttcct ggacttttgc tacacgttct
     4921 tctggctgcc gggattgata ctggcttttt ttgggtatta ctggatcgta ggccccatga
     4981 cattgctggt aattccgctt accctcatga attattcaat attatacgct tatcagttat
     5041 acgtattcag acgcttgaac ctaagggtaa gaaaaaacat gcggggtttc atattattca
     5101 taattgttta tcagatgata atgtctcctg tttctgtatg gggatacctt caggaattcc
     5161 tgaagctgaa gagaaactgg aaatagcagg atttcccggg ctgctgcaaa accacatttt
     5221 gcagcagcta catatttttt tacaggtttg tacaaaataa cagaaatgaa aattcttgat
     5281 gccggggagt ggttccatgc ctgcaatcaa tgaagaagac agcctctaca ataaaactgc
     5341 cgatgtatca agtgataata tccgctctat ccttggaagc aatgcagatt ttgtatcccg
     5401 tgtcattctt gtcaggaata aacctgaaat accctctgtt cttgtataca tggacgggct
     5461 ggtagacatc agtctgataa atgataatat actgaagccc ctttcccagg aggagcagtt
     5521 tgattcctgc agcggggaag aagatgccgc aaggcttata agcatgggaa agatatattc
     5581 ctcctcaatg aaggtaagga caaatatgaa agacctgact gacgatatac tgacaggaag
     5641 cgcggcattg atattcaatg atataaggac agcctttacc ttcgaggcca aagcttctct
     5701 aagccgaacc atctcagagc ctacagggga aaccgttatt aagggcgcaa aggattcttt
     5761 tattgaggac ttaaggacca ataccgctct gtgccgcaaa aagcttacct ctccatacct
     5821 gactattgaa cagattcgtt tgggaaaaca gacaaaaagc aatattgcga tattatatgt
     5881 gaaaaatata gcaaaaagca aattgataga tgaagtgaaa cagaggcttg aatccattga
     5941 tattgacagg gtgcttgacg caggtgttat tgagggggca atcgttgatg acaagtattc
     6001 cgcattccct cagattcaat ataccgaaag gattgacaga ttatgcgcaa gccttgttga
     6061 gggccgtgca ggaatactga ttgaaggcct gcctacggct tatatagttc cgggcacact
     6121 gctgcaattt ttgcaggctc cggatgacta ctcctttcag ttcattattg catccattgt
     6181 ccgctttatc aggtttatga gcatgtttat aacactggtg cttcccggct tctatatatc
     6241 cattacctcc tttcacccgg aaatgctgcc tgcggaactg gccttctcaa tagttgcatc
     6301 cagggtgggg gtgcccttcc cgatgtttgt ggaagtactg atactgctgc tggcttttga
     6361 gctgttgatt gaagccggcg taaggctgcc aaatgtcata ggccagacag tgtctattgt
     6421 gggcggtctt gtggtaggcc aggctgccgt tgaagccaaa ctggtatccc cggctaccgt
     6481 tatgattata gcaattactg ccattgcagg cttccttatg cccaaccagg atttcagcaa
     6541 tgccataagg ctatggcgct ttatactggc tatattcggc agcataaccg gtatatttgg
     6601 gctcaccttt gggttgatat tccttctcta ccattggtgc aggattgaga ccttcggtat
     6661 tccgtacctg gacccctttg ttgccaatga ggatgaacag cttcaggaca ccattttccg
     6721 gcttcccctc ttcaccatga aaaagcggcc cagcagcctt gatcctaaaa acaagaaaag
     6781 gatggagtga gctatatatg cggaaatatg caattttgac agtcctgctg gctttgacgg
     6841 cggctttatc cggctgtatg caaatcataa tgggaagatc ggaaattgac aagctgttta
     6901 taatacggat gttctctttc gatgaagcag aagagggcaa gatcaaagta accgctacta
     6961 caaaggatct gtcgacggga gaaggcggag gagaggtgct tcagaaaggg gaaagtattg
     7021 cagccgaagg agaaactgta tttgatgctg tacgaaatat gtcaacatac gcagacagaa
     7081 agctgaatgc aggacatacg gaattcatac tcttcggaga gtcccttgcc aggaaaggca
     7141 tactccccta tcttgattta ataagcagac acccggaatt ccgttataat gcaaaacttt
     7201 atgtagttaa aggaggtacc gcaaactccc tggccgaaaa ggcaagcacc tcgaaatact
     7261 ttgtagggga caggttagcc catatagaag aaaatatcgg cagaacatcc atatccggta
     7321 cggtcacgct taatgaagta ctgcttatat ttgatgaaag atatctggat accttcatcc
     7381 catatattga gccggttcct acaaaggcaa ccggtgaaaa accggatacc tgcgatatgt
     7441 tattgaacgg ttatgcaatt ttccgggagg ataagcttct ctacttcgct tcacgggaag
     7501 aatcccgggg gataaactgg attatgaacc gcatcggttc agggataatt gtgttaaagg
     7561 accaaacggg gcaagaaatc tccatgataa tagtcaaagg agcttcaaga gtaatacctg
     7621 agattgtagg gaatgaactc cgctgcaggg tggaaatctc tttctcaacc aatataggtg
     7681 agataatggg cggaagaagc gtatttgaca aggcttccct ggattacatt actgagcagc
     7741 aggaaaattc tataaagcga gaggtggaaa atgccataag gatagcacag gataagaatt
     7801 ccgaccactt cagcattatt tcaaaattca gaatcaaata tcctctgctc cgggactatt
     7861 ttcagcaaaa ctggcaggag ctgtttccag atatcaaatt tgaagtcagt gttgattccc
     7921 taatagacgg aacctacatg ataaatgagc cggttactgc cagtaaggag gctgtgggtg
     7981 aatgaaaata tttctgatgg tggtaatagt tgtaattgcc ataacagcag agatgatacc
     8041 ggttataaaa aagaagcagc acaaggaggc agcagtcctc attatgctgg gggctgtgac
     8101 tctggcctac ggctattatt acattaccca tgtgcacaca gccagcctgg caaatcttat
     8161 gtttgacata ttccgcttaa ggtaaggaga ttcttctatg aaaacaggca aagataaaat
     8221 atcggttaag cagcttttct ttgtgtttac aataatggtg tcttctccca caacaagact
     8281 gcttcccaaa tatgcggcgg caaaagcgga gcaggcaggc tgggtgtccc cgattataag
     8341 cacggtgccc tttattctgc tgatactggc tattgattct ctattgaaga aatataaagg
     8401 gcagagtatg tctgatatta tcagcaatat tctggggaga ttccttggaa agctggtact
     8461 ggtggtatac ctgctgtggg ctctctggct tgcggccatg tatacacgtt attattcgga
     8521 acgtctgacc agttcgattt atcccaatat cagcgttggg ttgttcatta ttttagccct
     8581 tatacccata gcctacatac tgcgttccgg cttcaccccc attgcgagga tgagtgaaat
     8641 actgctgccc ttcatagggg caatgcttat catgctggcg ctcttcttat ttcccaaggt
     8701 tagagcagat aatctgctgc ctgtctattt caacgacata attcctatat tcaggggaag
     8761 tatcgcagta acagggatac tgtcctatct gttccttatg ttcttcctga gcgacaatgt
     8821 tgtaaatctg aaaagcctca gagtgttcgg ctatattgct gcttatgtga atatttcaag
     8881 cataatgctg gtaaatttca ttgtaatagg cgtaatgagc agttcccttg ccaggagggt
     8941 ttccgtgcct gtccttactg ttgtaaaaca aattaccata atggatatta tagaaaatat
     9001 tgaggctgta gtcgttgcca tatggatttt cgccgacttc acaatgatta caacacttct
     9061 tgtaattgtg ctcaacctta ttaagtcaat atttaacttg tctgatacca ggcttctgat
     9121 taatatgctt gctgtactgg tatattttct gtctatggga gtatgtgcca gaaggtttga
     9181 gcttgaggat ttcaccaata gcctgctcat gcccataaat attacactgg gctatgtatt
     9241 cccctttgta ctattcgctc tatccaagct caaaaaaaca actaaccctt cccgataaaa
     9301 cggaagggtt agttgtttac agatcatatc aggttttact gcggattgta caagcccatg
     9361 ctctccatat acttgaagac actttctcca tgctgctgct cttctttctg tatatggttc
     9421 agagtctggc ggatgtttgg atccctgaat tcaaatatag ccgtatcgta tgcccctgaa
     9481 acatatttct cagtcatgag aatatcgttg cacaggtccc cgtcactttt gcttccggtc
     9541 cccgtcctta tgggctgctg tccctgtgcc ccctgccggc cttgctgctg attctggttc
     9601 atgcccggca tctgtccgct taacagctgg ttgatggtat taagatgctg ctgctcgtgt
     9661 tttgcatgag agctgaaaag ctgcttcaac tgagggtctt gagccttgcc ggcatattca
     9721 ttgtatttct taatgcatat ctcctcatgg gttttctggt cctgaagcag ccccttttcc
     9781 ttttgagtca gattgttcat aaaatcacct ccggctttag tatttttcat tgtaagcaaa
     9841 atatactaaa agcctttgat ttcaccctcc ataactgtta caattacttt atattcaata
     9901 taggagccgt catctgccgt tattctgtaa acaaccttgc ggctgaaatc attaggtgtt
     9961 ataccgctgt actgctgcac ttccccgaca tataccgact ttcccagata agagaatgaa
    10021 gcaatcaagt ttgacacaat tgtatcagat ggaacagaca ctctgattat acggttctgc
    10081 tgattgataa ttcctcttgc ctttggattc aagcctgcaa attcaaattt tgacaatatt
    10141 ttttcatttt catctgcagc tctgactgtt acggtatagt tacgggttga gccgtcctgt
    10201 gccgcaacag tatatatcac cggattggta aaatccttta ctgtctctcc gcttttctgc
    10261 tcatcgcttc caatcaggac tttgctgttt tccgaactga caaatactgc cgccagtgca
    10321 ttcagtgaag ttccaaaggg tacggtaacg tttatcttgc tctcagcctc atttataacc
    10381 ccttccgctt cgggaacaga gaatccgaat tccttaataa gcttttcact gctgggagca
    10441 gcattgacta tcacagtata ttccctggca gtaccgtcct gtgccgttac aatgtacttt
    10501 acaggatttg taaagtcttg cactgtctct ccgctaacct gctcctcccc tttcaccttt
    10561 acaacagctt ccggtgaagc cttgaaggat gcaacaagct tggttctgtc tgtaccgtaa
    10621 ggtactgtca gttctatagt cttttgctcc ttgcctactt ctgccctgat aacaggttcc
    10681 aatgctctga atgcgaaaac ggttatccgg ttttcagtgt tttgagcttt tttcactatt
    10741 acggtatact cctttgttgt tccatcctcg gccgttacta tatattccac cggttcactg
    10801 aaatccggct ctttacccgt cttgacagat atcctggtat tgtccggtat atctataaca
    10861 ggcaccagct tgctcaggtc tgttctgtac ggcactgtaa cagttataat cctttttccc
    10921 tcatctatct ccccgatggc ggacttaggc ttttcaaacc tgaaggcttt tatggatttg
    10981 tcaagctcct ttgcaatggc caccactacc ttataatcct gtgaagatcc gtcctgggca
    11041 ataacggtat aggtaacttc cccggaaaag tcattggtgg tttccccgct gatctgcctt
    11101 tttcctttca cctctaccac agcatgctcc gagcacttga agcttgcagc cagcttcttc
    11161 acatccgttc catggggcac tgtcaggctg attctctttg catccttatc aatatttccg
    11221 tctattccgc ctgcaatgtt attgaagccg aagcttgtaa tctccttgga agaggatttt
    11281 aggggcggtt ccggcttatt gtcatcatcg gctatgccgc gaaagctata tgatatcccg
    11341 tcagctgtta ttctgtattt aacattctta tacagcggtt tttttgatgt aactctgaat
    11401 acgttactgc tgatcctcga agtactgtca atcaggtttt ctcctacggc attaccgtct
    11461 ccgtccagcc tgtacaggct gcttaccctg gatatcctgc tgcttttgta aaccctgaga
    11521 ttgtattcat tcagcaaatc cacaccctgg atataatcac tgctcattat actggagcct
    11581 tcaaaatcta tctccagatc atcgatatcc agcttgttcc ctgcaatatc cgccgcagcc
    11641 gacttaggct ccaggatata atagtattcg tcactcatct tatcctcagt atatatattt
    11701 accctggtat tatcttcagg atcaacttcc acgaagtcaa tatcgatagt cctcagcttg
    11761 ccgtcgtcat cctcataagt tatcttatag ctccccggct cccttatggc ttcgctgaat
    11821 tccacctgaa cctttttgca gttaatggct tcaacatctt cnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn ngcatagtct tctaccattt cagtaaaatt gaatctgaat
    12001 tctttgtcag ggggtatatt tttattctta gctatatagt ccacagtgca ataagcttca
    12061 tcatcatccc catcatcggg attaagcact gctttccatt ctatacctac ccctccggga
    12121 tttttgattc ctgatactcc atctccttta agcagtaccg gttccgtgaa aactacccgg
    12181 aatcttctca catccatctg atcccagtca tctacttcag gagggtcatt ctcatcatca
    12241 gtaccatcga attccaggcc gtactcaaac tcatatttat tgcctctcat gtcccatatg
    12301 cctgtaagtt tggttacctc atactctcta tcatctttca gcgattcatt tccatctata
    12361 accatcacaa ccacagtccc gtcatccagt acacttcccg cctgtttgaa atcaatgcct
    12421 gacgcttcca tcccagcgtt ttccgcatac acttcctctt caaaatgtat ccagacctct
    12481 ttcctgttta cagcctctat ataatccact tccggaggat catcgtcata cacgctgccg
    12541 ttataaagaa tatctacgct tacatttgaa agcctgtttc ctgataaatc cttgatatta
    12601 ttcatcatca cggtatgatt tctgtctgaa gacagggaac tgaccgtaag cctgaccgtt
    12661 tttccgtcct cctgcagtac tgcctttgta acgatagcaa gcccgtctat cctgtagttg
    12721 actatgttct ccgccgatcc ctcatccaag gtatcgtcga agtgcaactc cagagttctg
    12781 ctgtctatac actgaacaga ggttatatat ggtggagttc tgtcctcgcc tattcctttg
    12841 aacctgtact ttttatcgct gcttgaagac ttcatttcat ttccgaattc atcataaatg
    12901 ccgctgattc taagtgtata cctttcattt ttttccatct cttccgtaat cagcagcact
    12961 gtcctgcccc ccactgaata caaatcatcg ggattcttta ttcttgcatc caggatctcc
    13021 aggccttcat ctatctcata gcagtcaatg tcgcaggctg attccacatc aagggcattg
    13081 gaatccgaaa actcgacttc taccattgtg tttgtgactg cccttggatc attggcaact
    13141 gttggccccg atttatcctt tgacttccct ctgaattcct tttttattat cctggttatg
    13201 ttgtttccca ataccgagtc atccatcata tcttcaatta tcagagtata tttagtgcct
    13261 gcttcctggc tttcagtcac aagatacacg gattcccaca aaccgtcctc atccatgtct
    13321 ttgacctcag ccgtttccac ctccaggtcc ccgtcgccgc cggttatact atagttgttc
    13381 ttgtcctgag ccgtggattt atccaatccg ttgctgtccg aaaactcaag aagcagcctt
    13441 acattgttca gaaccgtaag cttactcagc tttggagggc ttgtatccct tctccctgta
    13501 aatctttttg tgattgactt tgttaccgct ccgtcgccat tctttatatt gcgtatttta
    13561 agctcgtagg attttcctga ctcaatgccg ctggtagtaa gccttacctt ggtatttgtg
    13621 ctgtccagct ccgccgacct tacgcttgct cctgaaatcg tataagtatc cgtatcctgg
    13681 gctgtttcat tatccaatac tctgtcaaag gtaagctcca gcaggtctgt atccctgcat
    13741 tcgatcttaa tgagttttgg agaagtctta tcctttttca ggcccctgaa gctatagtcc
    13801 ttatcattga attccaatgt gtaggaagtg ttttccttca ttgccgaggt atctattatt
    13861 acagcatatt cattcttcat acttactttg tttatgtcaa gatgcttaat ttcatacaga
    13921 tcctcatctt ccgcatcggt accgtcaatg gactccttaa aaacaacctc caccttgctg
    13981 tttgaaatag cccttacgtc ttttacctct gcctccaatt ccttggagta caagcctgct
    14041 gatattgcat cccacttatc cacatcccct atatccacca ggtattctat aagagtcctg
    14101 cgctctccct tcattttggt attaagagcc tcaaccgtag caatcgcaag gctttttact
    14161 gtaaagttcc tgataccggc taccttgtac agcccctttt cttctgcaaa atcaagcact
    14221 tcatcccagt caaagtctcc cttaccgtca attttctgct tatagcccag gctttccagg
    14281 agtaccttgt aatatgccct cgaatctatc aggctgtaag gcatgaagtt cactccgtca
    14341 ccaatccacc caagctccgg gtgatttttc aggtaactca gcacgtttct gccttcttcc
    14401 caggcaacta cgccggcatc cttgaagttt tttcctccgt aatagtctaa tgcatcctgt
    14461 tccaaacctt taagcctcag aaacattatt gccgcctgca gcctgctggg gcgagtttca
    14521 aggtattctt cgtctacaat tccggtatgt cccttcagta ttcccaagtc tctgcaaata
    14581 cgcccctcgt agccgatatc atcatcggca ataacctgta ccggaagtat tgcgcttaga
    14641 accagcgcaa aaattgtaat aacagttaca aattttttga ccatcaaacg tacctcctta
    14701 aaatatcagt gcctaccgat aaaaatgtcg atggtgcacc cctttaatgt taaatggcag
    14761 atactcgcct aataattaga tatgactctt ttcatgcaaa ctgtactgct ttgatgattt
    14821 gtaacatact cttaacagcc ctgtaatatt tggcggcacc atctgtatcc tccttatttt
    14881 atagtagttt ccattttctt tcaataatta cagttcgtcc catttctact atattttcag
    14941 ccgacagtca attgccgtat aatgctgtaa cttttcgcag gctatgagat taaatttgat
    15001 tgatccggta ataatatgga acgaaaacaa tccggtgcgg ttgaaataat acaagactgg
    15061 ccaggctgta gtatatggcg ggcatacttg caattaccga tcatttgatg tacaatttca
    15121 atacaatata aaaatatggt agagtaggct tacatgcgta aagtgctggt tgtacgggaa
    15181 gttgacacca gacgaaaagc ccgccagggc ttgcggtatg tatgctgcat tccgctgcca
    15241 cttaggagcc atccctcttt aagtggtcta tttgccattt aaagagagga ggtgattgta
    15301 tgagtaaagc taagacaatt gcttttgtcg gattgctcgt atcaatggaa atcatattta
    15361 cccgttttct atccattcag actccaataa taagaattgg cttcgggttt atacctattg
    15421 cttttactgc catactgttt ggtccgctta taggtggaac ggctgctgca attgctgata
    15481 tcacaggcat gatgattttt cccaagtttc cgtttttccc cggattcaca ttgagcgcat
    15541 ttttaaccgg cgcaatatat ggcctgtttt tatacaggaa accgattaca atagtcaata
    15601 taggaaagag cgttttgctg attactattc ttgttgatct cggactgact tcactttggg
    15661 tatatatgac tacgggaaac gctgcatctg tattcctgat gccgaggatt ctgaagagtg
    15721 ccataatgct tcccattcaa atagtgacaa ttaatgtact gtggaaatat atcggaagcc
    15781 atatcgacaa agcgcgctat gcaaaagaac attaaaaagg cttggagccg gtcggctccg
    15841 agcctttttc acataagaag aataatgcga cgcatctgta gtttacataa gcctccgcaa
    15901 gagacaatat agtatatcga cgcacacgga ttatgatttt gacaggacta ctgtgaagca
    15961 aaacaaatta atacgaatcc gcctcacgcc ggttaatttt tcgcttttct tcgttatcct
    16021 cattgattgg cgccagctaa tctgcttcct ctgcctcgaa aatcaacaaa tttcctcggc
    16081 gtgaggtcaa tagattttcg agcaagctga tttgatttgt ggcaaggcgg gcgctc
//
LOCUS       DAOQUR010000049        16113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4120_length_16113_cov_11.129530, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000049 DAOQUR010000000
VERSION     DAOQUR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4120_length_16113_cov_11.129530"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..886
                     /gene="ftsE"
                     /locus_tag="PKI50_09535"
     CDS             200..886
                     /gene="ftsE"
                     /locus_tag="PKI50_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNT03982.1"
                     /translation="MIQLRSVSKEFADGNKVLRNINLTINNGEFVFLVGSSGAGKSTI
                     IKLLLKEMEPTTGTIIVGDKDITKYKRREIPFHRRNIGVVFQDFRLLQEKTVYENVAF
                     AMEVIEAPPKEIRRQVPIILSMVGLSKKAGLYPQQLSGGEQQRVSLARAMVNSPSILI
                     ADEPTGNLDPDMSWDIMKTLSEINQRGTTVLMATHASDIVDKMKKRVVEIENGIITRD
                     EMKGGYSSEA"
     gene            876..1772
                     /gene="ftsX"
                     /locus_tag="PKI50_09540"
     CDS             876..1772
                     /gene="ftsX"
                     /locus_tag="PKI50_09540"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNT03983.1"
                     /translation="MKLRTGKFFIREGFRSLKRNSTMSAAAITSVIAALLVIGIFFII
                     VINIDYAATKLESQIEMMVYLEDGLSENIIDTMETEIRSINGVKSVEFISKDTALSNL
                     EKNWGENSYLLEGLEGDNPLPDAFLITLSDPSDASGVALSVSSISNIEKVVYGKEELA
                     KLLNATYVMRMSSVVIILILLFISIFIIANTIKLTLYARRREIGIMKYVGATDWFVRM
                     PFIIEGIVVGMIGAVVSTVILGVGYYYCSGLVKNQMIGFMSISLIPFNQIIVSMVILL
                     IIVGVVIGAVGSLISVRKFIKV"
     gene            1784..2923
                     /locus_tag="PKI50_09545"
     CDS             1784..2923
                     /locus_tag="PKI50_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT03984.1"
                     /translation="MNKRIAAALIALMLSIPCANVYAKNPTEIDELQRNQDKLNQQIK
                     QMQSSINKIKTEKVSVAKAIEDLDSKLNQAEDELTGVENQLSQLENQIAVTTRELERA
                     SSEASNQKELLKKRVRVMYENGNVGYMSVILNSASFSDFISRVDFLKKIISFDMNLLS
                     KMKSYRDSVADKRSQLASELEEKERLKNEIGKKKEEVETAKQVKEMTLKEILSDLKEL
                     ERQEDKLLEQSKEIAKKIVELQSKEKYIGGELTWPAPGYYKITSQYGNRTHPILKKKK
                     MHTGIDIAVPSGTSVIAANAGKVIYSGYNGGYGNTVIIDHGGKISTLYAHNSKLLVKV
                     GDKVEKGQTISKSGSTGLSTGPHLHFEVRENGQHVDPMKYLTGKK"
     gene            3056..3997
                     /locus_tag="PKI50_09550"
     CDS             3056..3997
                     /locus_tag="PKI50_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNT03985.1"
                     /translation="MKKAITAVIILLLSAMALSGCMESGKKPADQRLKIYVSFYPMYF
                     LASEIAGDKAEVITMVPAGAEPHDWEPAPKTAAEISKADMLIYNGAGMEPWLDKILPN
                     INEKRTRLVDASQGIELLKSSEHDEEEEDIEENHEHGIYDPHIWVSPIRFRQQARNVY
                     EALLEIDPENSEYYGKRKIELDSRLSDLGKDIWDASKHFKSNTIVVSHESFGYFANDF
                     LLEQIAIRGINPQEEPSPSKMVELIRKCREKKVKYVFFEKLTSPKLSETLAREVGAGV
                     LVLNDAAGLSEKDIKSGKDYITVMYENLENLKKALGD"
     gene            3998..4750
                     /locus_tag="PKI50_09555"
     CDS             3998..4750
                     /locus_tag="PKI50_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNT03986.1"
                     /translation="MGAEIIRVENITFGYGKAKVLDNVSTSVIDGDFIGIVGPNGSGK
                     STLLRIFLGLIKPQKGTVRVFDTDIEKFNQWGKIGYIPQKATSFNQGFPATVEEVVSA
                     NLYPVVGLAGRIKKTHCEKVDEALQIVGMLEQKKSMVGRLSGGQQQRVFIAKALVSAP
                     RVILMDEPTVGIDQNSEESFYELMGFLNREKGITLVMVTHDIGAIDNRVNRVLCLSGG
                     KLYEHDCAVSRASDIFKEAYGKRDSLPIIHRH"
     gene            4774..5583
                     /locus_tag="PKI50_09560"
     CDS             4774..5583
                     /locus_tag="PKI50_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNT03987.1"
                     /translation="MFELFNYEFMQRALAAGLLVAVICPLIGTFVVMRRLSMIGDTLS
                     HASLAGIAAGMLGGLYPLWSALIFSLAAAIGIEKLRKHFPQYAELSISIVLSASIGLA
                     VVLISLANSFNADLMSYLFGSIIAVNALDIYIILGLSIIILVSVGLFYKEFFYMAFDE
                     EGAELAGMPVSSLNMYFTVLTAMTIVVSMRVVGMMMVSSLMVVPVACSLLISKSFKNT
                     IFLSVFFALISVVIGLFSSYWFDLAPGGSIVLTSVFILIAVIIVKRIFRMQ"
     gene            5614..6051
                     /locus_tag="PKI50_09565"
     CDS             5614..6051
                     /locus_tag="PKI50_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNT03988.1"
                     /translation="MKDIEKLETELKEKGYKLTEQRRSIIEAFNENPGHHTAQKIFEL
                     VKSKLKGINFSTIYRNLELLSTLEIISKLYDESGISHYELFGRTHHHHIICKGCGETR
                     EMDICPYAGLDEGQLSAMGFKATSHKFEIYGYCSKCRLLKEQD"
     gene            6149..7351
                     /locus_tag="PKI50_09570"
     CDS             6149..7351
                     /locus_tag="PKI50_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT03989.1"
                     /translation="MINKRAAIFWAIILVIVTAFGTFVVTNTIAISLGNKVVIAQKDF
                     EAIKSMDKLMGLKEYIKSNYVEGAEGNKLIEGAIKGMFESLGDPYSVYMTKEEFKNFN
                     ETTKGSYGGIGVIVSRSEDGYVTVVAPIEDTPGEKAGLKTNDKIIKVDDKDIVGIDLE
                     EAVALMKGKKGTKVTLTVMRDNVREPKIFEITREEIILKTVKSNMMENSIGYIRITMF
                     DEDTGSEFKKALKELTSQNMKGLILDLRQNPGGFINQCVDVADELLDEGLVVYTEDKA
                     KKREDYKSGKGKLEVPFVVLIDEGSASASEIVSGAVKDRKAGLLIGVKTFGKGLVQSI
                     EQLKDGSGIKLTTQKYYTPNGISINKIGIQPDIEVKAPEPEEGQNPEDVKDVQMERAV
                     AELLKQIK"
     gene            7367..8662
                     /locus_tag="PKI50_09575"
     CDS             7367..8662
                     /locus_tag="PKI50_09575"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT03990.1"
                     /translation="MFTVLKIMGLIFGQFGSFLMNELFWLIALIVILIYRKNSDIEAK
                     MLGSSYKLHYKVAGSFIVGLTAGLIGSLIVTLVGISIEDYTRSGGGSLIEGITYIWAV
                     AILLSAINPRYLCFSYAGGIIALMNLVFGFPSVNVPGLMALIGVLHLVESLLIWMDGS
                     SYSVPLFLRGKDGKTVGGYMMSRIWPIPLVAFAVILGIETGGVSLGGIIDMPGWWPLL
                     KHSAAGSRHGLAYLPLVVPVVLGYGDLALTRTPEQKCRNSALRLAGYSLVLITLSVIA
                     SKLRLFAFAAAVFAPVAHEMLIQYGVKEEEEGKPYFANKETGLRVLYVKKGSVACKMG
                     IVPGDTILRINGTALLSEKQLAEFLASCPVFIWVEFEKYEGELITAEYSDYRNGIGDL
                     GILIVPHNAKLYYEINRSSSLAKRFIKRFISNRNRDIDM"
     gene            complement(8628..8801)
                     /locus_tag="PKI50_09580"
     CDS             complement(8628..8801)
                     /locus_tag="PKI50_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT03991.1"
                     /translation="MKEENNEFIKVFNYFENANARNRDAFIELLNYTIKNKRGSKTMT
                     SSVTYQYPCSGLK"
     gene            8935..10044
                     /gene="ugpC"
                     /locus_tag="PKI50_09585"
     CDS             8935..10044
                     /gene="ugpC"
                     /locus_tag="PKI50_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNT03992.1"
                     /translation="MANLSLKHVKKVYPGNVTAVKDFNLDIEDKEFIVLVGPSGCGKT
                     TTLRMIAGLEEITEGELYIGDRLVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     LKKIPKAEIDERVRKAAKILEIEPLLDRRPKALSGGQRQRVALGRAMVREPKVFLMDE
                     PLSNLDAKLRVQMRTEIAKLHKRLNTTFIYVTHDQTEAMTMGTRIVIMKDGDIQQIDS
                     PQKAYDKPANLFVAGFIGSPQMNVFRGNVVEAGGKAALELPDGTALGLTDDAATRLKD
                     SGYVSKEVYVGIRPEHLHDAEVDPLTKDYSVITAKVEVAELMGSETYLHFTKCGVQAI
                     ARVDAKTSMRAGDTAEVHLHWDKIHLFDIETEKAI"
     gene            10129..11121
                     /locus_tag="PKI50_09590"
     CDS             10129..11121
                     /locus_tag="PKI50_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT03993.1"
                     /translation="MADLQRLLAQAARITEKRMAVITPNERLAWPEDSDIDITFEEAC
                     KNSGKNFRGWNIYMPEDAGTRFFLCISADSYYESEVGKLVMLLLNSAFEKDDSASAYL
                     KRALEGNLDAAGLSMLEENLKSCLPGYVLLIDNIENSRDEIIEILENTMDIKTELIYE
                     NRIIAVAEAENITESCSSFIKNVLSELLIECNAVIGGRAAKASELYPLYRNCMEALSL
                     KHIYGLGDNVLDYDGMYGYRVAYNLDPRLKEFLRRKVFTAEFSDMVKGELGITIEEFF
                     RNNLNLTDTAAKLYIHRNTLLYRLEKIYKCTGFDLKRFEDSWLFRLAWIVSRER"
     gene            11283..13265
                     /gene="uvrB"
                     /locus_tag="PKI50_09595"
     CDS             11283..13265
                     /gene="uvrB"
                     /locus_tag="PKI50_09595"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNT03994.1"
                     /translation="MDRFKLVSDYKPTGDQPEAIEKLAKGILAGEKYQTLLGVTGSGK
                     TFTVANVIEKVQKPTLVLAHNKTLAAQLCSEFKEFFPHNCVEYFVSYYDFYQPEAYVA
                     HSDTYIEKEADINDEIDKLRHSATAALLERKDVIIVASVSCIYGLGDPEDYRSLVLSL
                     RKGMVRDRDEVLRKLIDIQYDRNDINFVRGTFRVRGDIVDIFPAASSDKAIRVEYFGD
                     EIERILEIDSLTGNITGERQHVYIYPASHFATTQAKLEAAISNIEAELIERVKELKEQ
                     DKLLEAQRLLQRTNYDIEMMKEVGYCSGIENYSRHISGRAPGSSPFTLLDFFPKDYLL
                     IVDESHVTIPQVRGMYFGDRSRKESLVDYGFRLPSAFDNRPLNFEEFEQRLNQVIYVS
                     ATPAKFELERSTCVAEQIIRPTGLVDPEIEIRPVKGQIDHLIGEVRERAANNQRVLVT
                     TLTKKMAEDLTAYMKELDIRVRYLHSDILTLERMKIIRSLRLGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSEISLIQTVGRAARNVDGKVIMYADNITDSMRRAISET
                     NRRRKIQMDYNEKHDIKPQTIRKAIHEIIEATTAAEESAKYGIKHDIEFMRKDDLLRL
                     IAALEEEMKDCARALQYERAAELRDKIIELRHRLKK"
     gene            13277..16102
                     /gene="uvrA"
                     /locus_tag="PKI50_09600"
     CDS             13277..16102
                     /gene="uvrA"
                     /locus_tag="PKI50_09600"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT03995.1"
                     /translation="MAKDKIIIKGAREHNLKNIDVEIPRDKFVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDLDYIEGLSPAISIDQKTTNRNPRSTVGT
                     VTEIYDYLRLLYARIGTPHCHVCGKEISQQTVDQMVDYINTLEPGTRIQLLAPVIQGK
                     KGEHQKLLEEIKKSGYVRARIDGEIADLNEEIKLEKNKKHTIEVIVDRIIVKEGVNKR
                     LTDSLETVLKLSGGTVLIDIIGKEERLFSQNFACVECGISIEDPAPRMFSFNSPFGKC
                     PVCDGLGELRRIDPDLVIPDKTKSVLEGAIEPWKNLDETSWYYNVVNAAAKHYGFSMN
                     TPICELDEKAVQILLYGNGGEKIKVKYQREYGSGEVMMAFEGIVNNLERRYLETKSNY
                     MRYEIENFMSSVPCPECKGARLRKESLAVTLGGLSIAELCSLSVYKIMEFLGSLKLTE
                     KQRIISKLVLKELHERLEFLVTVGLEYLTLSRTAGTLSGGEAQRIRLATQIGSSLVGV
                     LYILDEPSIGLHQRDNGKLLKALRNLTDLGNTLLVVEHDEETMHAADYIIDMGPGAGA
                     HGGKIVAAGSFDTILACEESVTGQYLSGKKQIPIPQERRKPNGKWIEIKGARENNLKD
                     LDANIPLGVFTCITGVSGSGKSTLINEILYKELSHRLYGSKLRAGEHKWIKGIEHIDK
                     VIDIDQSPIGRTPRSNPATYTGAFDIIRGVFAATPEAKMKGYNNGRFSFNVRGGRCEA
                     CKGDGIIKIEMQFLPDVYVPCEVCKGKRYNRETLEVRYKGKNISEVLNMTVEDALEFF
                     KNIPRVQKKLQTLFDVGLGYIKLGQPSTELSGGEAQRIKLAYELSKRSTGKTVYILDE
                     PTTGLHIADIHKLLGILDRLVDSGNSIIVIEHNLDVIKRADYIIDLGPEGGDRGGTVI
                     AQGTPEEICKVETSYTGQFLKKILAKY"
BASE COUNT         4947 a         2926 c         4254 g         3986 t
ORIGIN      
        1 cttgagggac aggcgaaacg tccccgtgtc ccgcgtcccc gtgtcttttt gttgtttata
       61 aataaattat ttattacaaa acaaaaacat tggcattttt aaaggatttt tcttcatttc
      121 tgtagaagta taagttgttt gagtcgaatc cgcactatat ttgttttgta ttgacggttt
      181 tggtaaggag tgtgatagat tgatacagct gaggagtgta agcaaggagt ttgcagatgg
      241 caataaggta ttgaggaata tcaacctgac tataaacaac ggagaattcg tttttcttgt
      301 aggttccagc ggagccggca agtcaactat cataaaactg ctgctcaagg agatggagcc
      361 aaccacaggc actataattg tgggagataa ggatataaca aaatacaaaa gaagggagat
      421 accttttcac agaaggaata taggcgtagt attccaggat ttcagactgc tgcaggagaa
      481 gacggtatat gaaaatgttg cttttgccat ggaagttatt gaagctcctc cgaaggaaat
      541 aaggcgtcag gttccgatca tactgagcat ggtcggactg agcaagaagg caggccttta
      601 tcctcagcag ctttcggggg gggaacagca gagagtatcc ttggccaggg caatggtcaa
      661 cagtccttca atacttattg ccgatgagcc tacagggaat ctggaccctg atatgtcatg
      721 ggacattatg aagaccttga gcgaaataaa tcaaagaggt acgactgtat taatggccac
      781 ccacgccagt gatatagtgg acaaaatgaa gaagcgggtt gttgaaatag aaaatgggat
      841 tattaccagg gatgaaatga aaggtgggta cagcagtgaa gcttaggaca ggtaagtttt
      901 tcatcagaga aggatttaga agccttaaaa gaaacagcac catgagtgct gcggcgataa
      961 cctcggtaat tgcggcactg ttggttatag ggatattctt tattatagtg ataaatatag
     1021 attatgctgc aaccaagctg gaatcacaaa tagagatgat ggtatacctg gaagacggtt
     1081 tgagcgaaaa tatcatcgat actatggaaa ccgagatcag atcaatcaat ggtgttaaat
     1141 ccgtggagtt catatccaag gacacagccc tcagcaatct ggagaaaaac tggggcgaaa
     1201 actcctatct tttagagggg cttgaaggag ataatccgct tccggatgct tttttaatca
     1261 ccctttcaga tccttccgat gcatccggtg tggcattgtc ggtatcatcc atatcaaata
     1321 ttgaaaaggt ggtatacggc aaagaggagc ttgcaaaact cctgaatgcg acttatgtga
     1381 tgaggatgag cagcgttgtt attattctga tactgctgtt catatcaata tttattattg
     1441 caaataccat taagcttacc ctgtatgcaa gaaggcgtga aattggaatt atgaaatacg
     1501 taggggctac ggactggttt gtcaggatgc cctttataat agaaggtata gtagtaggga
     1561 tgattggagc ggtggtatct actgtaatac tgggcgtagg ttactattat tgctccggtc
     1621 ttgtgaaaaa ccagatgata ggctttatgt ccatatcact catacccttt aatcagatta
     1681 tagtaagcat ggtaatacta ttgattattg tgggggttgt aatcggagca gtggggagcc
     1741 ttatttcagt aagaaagttc attaaggttt aggaggggtg aaattgaata aaaggatagc
     1801 agcagctctg atagcactta tgctttcaat accctgtgct aatgtatatg caaaaaatcc
     1861 gactgaaata gatgagcttc agagaaatca ggacaagctt aatcagcaga taaagcagat
     1921 gcagagcagc attaacaaga taaagactga gaaagtaagt gtggcaaaag ccatagaaga
     1981 tttggacagc aagcttaatc aggctgagga tgagcttacc ggtgttgaaa accagctttc
     2041 ccagttggag aaccagatag cagtgaccac aagggagctt gaaagggctt caagtgaagc
     2101 ttcaaaccaa aaagaactgc tgaaaaagag agtcagggta atgtatgaaa acggaaatgt
     2161 cggatatatg tctgtaatac tgaattctgc aagcttctcc gatttcatat ccagagtgga
     2221 ttttttgaaa aaaatcatca gcttcgacat gaatctgctg agcaaaatga aaagctaccg
     2281 ggattcggtt gcagataaaa gaagccagct ggcatcagag ctggaagaga aagaaaggct
     2341 taagaacgaa ataggtaaga aaaaggaaga ggttgagaca gcaaagcagg tcaaagagat
     2401 gaccctgaaa gagatactca gtgacctcaa ggaactggag cgccaggagg ataagcttct
     2461 ggagcagtct aaagagattg caaaaaagat agttgaactg cagtcaaagg aaaagtatat
     2521 cggaggagag ctgacctggc cggcaccggg ttattacaag attacatcac aatatgggaa
     2581 tagaactcat ccgatactta agaaaaagaa gatgcatacg gggatagata tcgcagttcc
     2641 cagcggtact tccgtaattg ccgccaatgc aggaaaggtc atatactccg ggtacaacgg
     2701 aggatatggg aataccgtaa taatagacca tgggggaaaa atatccaccc tttatgccca
     2761 taacagcaag ctccttgtaa aagtaggaga caaggtcgaa aagggccaga caatatccaa
     2821 gtccggcagt accggacttt ccacaggccc gcacctgcac tttgaagtaa gggaaaacgg
     2881 ccagcatgtg gatcccatga aatacctgac aggcaaaaag tagggtcacc atacatggtg
     2941 acagaagcaa aaatacaccg aaaggtgtat ttttttgcat atttgcataa ttgacacagc
     3001 cttgggcata ttatacaatg taaatgcaaa tgatttgcaa aaaggagcag aggcaatgaa
     3061 aaaggcaata actgcagtaa ttattttact attgtcagcc atggcgctgt caggctgtat
     3121 ggaatccgga aagaagccgg ctgaccaaag attgaaaata tatgtcagct tttaccctat
     3181 gtattttctt gcaagtgaaa tagcagggga caaggcggaa gtcataacca tggtacctgc
     3241 gggagcggaa cctcatgact gggagcctgc tccaaagacg gcagcggaga tcagcaaggc
     3301 tgatatgctg atatataacg gggcgggtat ggaaccatgg ttagataaaa tactgcccaa
     3361 tattaacgaa aaaagaacca gattagttga tgccagtcag ggcatagagc ttttgaagtc
     3421 ttcggagcac gacgaggaag aagaagacat agaggaaaac catgagcatg gaatctacga
     3481 tccccatata tgggtaagcc ccataagatt caggcagcaa gcccggaatg tttatgaggc
     3541 gcttttggaa attgatcctg agaattcgga gtactatgga aaaaggaaga tagaactgga
     3601 ttcaaggctt tccgatttgg gcaaagatat atgggatgct tcaaaacatt tcaaaagcaa
     3661 tacaatcgtt gtatcccatg agtcctttgg ttactttgca aatgattttt tattggagca
     3721 gattgccata agaggaatca atcctcagga ggaaccaagc ccctcaaaaa tggttgagct
     3781 tatcagaaaa tgcagggaga agaaagtaaa gtatgttttt tttgagaagc ttacaagccc
     3841 aaagctctca gagaccctgg caagagaagt gggagcaggg gtcctggtgc ttaatgatgc
     3901 ggcaggcctc agtgaaaagg atataaagtc cgggaaggat tatataactg taatgtatga
     3961 aaatcttgaa aacctgaaaa aggcattggg tgattaaatg ggagcagaaa taataagagt
     4021 tgaaaacata accttcggat acggtaaagc caaggtgctg gataatgtca gtacaagcgt
     4081 tattgacgga gattttatag ggattgtagg cccgaatggc tcgggtaaaa gtacgctgct
     4141 caggattttt ctcggtttga taaaacctca gaaaggtact gtcagggttt ttgataccga
     4201 tatagaaaaa ttcaaccaat ggggcaagat aggatacata ccgcaaaaag ccacttcctt
     4261 caaccagggc ttccctgcaa cagtggaaga ggtggtttcg gcaaacctat atcccgttgt
     4321 aggcttggca ggacgcataa aaaagactca ttgtgaaaag gttgatgagg cacttcagat
     4381 tgtagggatg ctggagcaaa agaaaagcat ggtcggcagg ctttcaggag ggcagcagca
     4441 gagggtattt attgccaaag ccctggtcag cgctccccgg gtgatattga tggatgagcc
     4501 gacggtaggc attgatcaga attctgaaga aagcttttat gaattgatgg gattcctgaa
     4561 cagggaaaag ggcatcacac tggtaatggt aacccatgat atcggtgcca tagacaacag
     4621 agtaaacagg gtattgtgct tatccggcgg aaagctttac gagcatgact gtgcagtgag
     4681 ccgggcttcg gatatattca aggaggcata cggaaagcgc gatagcttgc cgataataca
     4741 cagacactag gtaataatgg aggagtataa cttatgtttg aattattcaa ttatgaattc
     4801 atgcagaggg ctcttgcagc aggattgctg gtggctgtga tctgccccct tataggtact
     4861 tttgttgtaa tgagaagatt gtctatgatc ggtgacacac tctcccatgc ttccctggcg
     4921 gggattgccg ccggcatgct cggtgggctt tatcccttat ggagtgcact gattttttct
     4981 ttagccgctg caataggaat tgaaaaattg agaaagcact ttccgcaata tgcggagctt
     5041 tcgatatcca tagtgctttc ggcaagtata gggctggctg ttgtacttat cagccttgcc
     5101 aattccttta atgcggatct tatgagttat ctgttcggaa gcataatagc ggtgaatgca
     5161 cttgatatat acataattct gggcttaagc attatcatac tggtatcggt agggcttttc
     5221 tacaaggaat tcttctacat ggcctttgat gaggagggcg cagaattggc gggaatgccg
     5281 gtatcaagcc ttaatatgta ttttacggtt ttgacggcca tgactattgt tgtctccatg
     5341 agagttgtcg gcatgatgat ggtatcctct ttgatggtag tcccggtagc ctgcagttta
     5401 ttgatatcaa aaagctttaa gaatacgatt tttttgtcgg tattctttgc attgatttct
     5461 gtagttatag ggcttttttc atcctattgg ttcgacctgg cgccgggagg ttccattgta
     5521 ctgacctcag tatttatact tattgctgta ataattgtta aaagaatatt caggatgcag
     5581 tgattctgcg gagctgacca aggaagtgcg aaaatgaagg atattgaaaa gctggaaacc
     5641 gaattgaagg agaagggcta taagcttacg gagcagagaa ggtccattat tgaggctttt
     5701 aatgaaaacc ccgggcatca cactgctcaa aagatatttg agctggtaaa gagcaagctg
     5761 aagggaataa acttttcaac catatatagg aatcttgagc ttctgagcac tcttgaaatc
     5821 atcagcaagc tgtatgatga aagcggcatc agccattacg agcttttcgg aagaacacac
     5881 catcatcata taatatgcaa aggctgcgga gagaccagag agatggatat ttgcccatat
     5941 gcaggactgg atgagggaca gctgagtgca atgggcttca aggcaacaag ccacaaattt
     6001 gaaatctacg gttactgcag caagtgcagg ttattaaagg agcaagatta agagtaagta
     6061 caaattaatg attgagataa tatatgaagg atgataataa tataataaga agaggtaaga
     6121 attctgattt ttttatgagg tgaaattgat gataaataaa agggcagcaa ttttctgggc
     6181 aataatactg gtaatagtca cagcctttgg cacttttgtc gttacaaaca caattgctat
     6241 cagcctgggg aacaaggtgg taatagctca gaaggatttt gaagcgatta agagtatgga
     6301 taagctcatg gggcttaaag agtatatcaa gagcaattat gtggaaggcg cagagggcaa
     6361 taagcttata gagggtgcaa taaaaggcat gtttgagtcc ttgggagacc cctattctgt
     6421 ttatatgacc aaggaggaat tcaagaattt caatgaaacg accaaaggct cctatggagg
     6481 catcggagtc attgtcagca gaagcgagga cggctatgtg acggttgtgg ctcccataga
     6541 ggataccccc ggagaaaaag cggggctcaa aaccaatgat aaaattatta aggtggacga
     6601 taaggatata gtcggcattg acctggaaga ggcagttgct cttatgaaag gcaaaaaagg
     6661 cactaaggtt acattgaccg ttatgaggga taatgttcgg gagcccaaga tattcgaaat
     6721 aaccagggaa gaaattatcc tcaagacagt gaaaagcaat atgatggaga atagtatagg
     6781 ttatattaga ataaccatgt ttgatgagga tacaggaagt gagtttaaga aagctttgaa
     6841 agagcttact tcacaaaaca tgaagggttt gatattggat ttgaggcaaa accccggagg
     6901 ttttataaac cagtgcgttg atgtagccga tgagcttctg gatgaggggc ttgtagtata
     6961 taccgaagat aaggcgaaga aacgggaaga ttataaatcc ggcaagggta aactggaagt
     7021 gccctttgta gtgttgatag acgaaggcag tgccagtgcc tctgaaatag tttcaggtgc
     7081 ggtaaaagac aggaaagcag ggcttcttat cggtgtgaaa acctttggca aaggattggt
     7141 gcaatccatt gagcagctca aagacggttc cggcataaag cttacgaccc agaagtacta
     7201 tacacccaat ggcataagca taaacaagat aggcatacag ccggatattg aggttaaagc
     7261 cccggagccg gaagagggac agaaccctga ggatgtgaag gatgtacaga tggaaagggc
     7321 tgtagcggaa ctgcttaagc aaataaagta atttgcgggg tgctttatgt ttacggtgct
     7381 taagataatg gggctgatat ttggacaatt cggcagcttt ctgatgaatg aattattctg
     7441 gctcatagcg ctgatagtga tattgattta caggaagaat tcggatattg aagcgaagat
     7501 gcttggcagc agttataagc tgcattacaa ggtggcaggc tcttttatcg tcggattgac
     7561 ggcaggcctt ataggaagcc tgattgtaac gcttgtgggt atcagcatag aggattatac
     7621 caggtcggga ggaggctccc tgatagaagg cattacttat atatgggcgg ttgctatcct
     7681 gctttctgca atcaatccca gatacctgtg cttttcctat gcaggaggaa ttattgccct
     7741 gatgaacctg gtgttcgggt ttccgtcggt aaatgtcccg ggcctgatgg cactcattgg
     7801 agtactgcac ctggttgaaa gccttcttat atggatggac ggcagcagct attccgtccc
     7861 tttattttta agagggaagg acgggaaaac cgtcggcggt tatatgatga gcagaatttg
     7921 gcctattccc ctggtggctt ttgctgtgat actcggaatt gaaacaggcg gagtgagcct
     7981 tgggggtata attgacatgc ccggatggtg gcccttatta aagcattccg ccgcggggag
     8041 ccgtcacgga ttggcatatc tgccccttgt agtgcctgta gttctgggct acggggattt
     8101 ggccttaacg agaactcctg aacaaaaatg caggaattct gctttaaggc ttgcaggcta
     8161 cagcctggtg cttatcacat tgtcggtgat tgcttcaaag ctcaggcttt ttgcatttgc
     8221 agccgcagta ttcgctcctg ttgctcatga aatgctcatt caatacgggg taaaggagga
     8281 ggaagagggc aagccatatt ttgcaaacaa agagacaggc ctgagagtgc tttatgtcaa
     8341 aaagggttcc gttgcatgca aaatgggaat tgtgcccgga gacacaatat taaggatcaa
     8401 tggaacagct ctgctcagcg aaaaacagtt ggctgaattt ttagcctcct gccctgtttt
     8461 tatctgggtg gagtttgaga agtacgaagg tgaattgatt actgccgagt actcggacta
     8521 caggaacgga atcggagatt tggggatact gattgtccct cataatgcga aattgtatta
     8581 tgagataaac aggagcagtt cccttgccaa gaggtttatt aaaaggttta tttcaaaccg
     8641 gaacagggat attgatatgt aacggatgaa gtcatggttt tcgaacctct tttgttttta
     8701 attgtataat tcagaagctc aatgaaagca tcgcggtttc tggcatttgc attttcaaaa
     8761 taattgaaaa cctttataaa ttcgttgttt tcttctttca catccatacc tcccatggac
     8821 attttttatg aatttctaca aaaatacccc catacattca ttatttctcc caatggaatt
     8881 aatattcccg atgaaatata cttaatttat caaatagcaa gggagagaat tgaattggca
     8941 aatctgtcct tgaaacatgt gaaaaaggta tacccaggaa atgtgacggc ggtgaaggat
     9001 ttcaacctgg acattgaaga taaggagttc attgttcttg taggaccctc gggctgcgga
     9061 aagacaacaa ccctgaggat gatagcgggg cttgaggaga ttacggaagg ggagctttac
     9121 atcggcgaca ggcttgtaaa tgatgtagcg ccaaaagaca gggatatagc catggtattt
     9181 cagaactatg ccttatatcc ccatatgacc gtttatgata atatggcctt tggcttgaag
     9241 cttaaaaaga ttccaaaggc tgagatagac gaaagagtca ggaaggctgc aaagatactt
     9301 gaaatagagc cgctgctgga cagaaggccc aaggcattat cgggtgggca gagacaaaga
     9361 gtcgccctgg gaagggcgat ggtcagggag cctaaagtgt tcctcatgga tgagcctctt
     9421 tcgaaccttg atgcgaagct gagggttcag atgaggactg agatagcaaa gcttcataag
     9481 aggctgaaca ccacctttat atatgtaacc catgaccaga cagaagccat gacaatggga
     9541 accagaattg ttataatgaa ggacggggat atacagcaga tagacagtcc ccagaaggca
     9601 tatgacaaac cggccaatct atttgttgca ggcttcatag gcagccctca gatgaatgtc
     9661 ttcaggggca atgttgttga agcggggggc aaggccgctt tggagcttcc tgacggaact
     9721 gccctgggtt tgacggatga tgcggcaaca aggcttaaag attccggcta tgtttcaaag
     9781 gaagtatatg taggcatcag gcctgagcat ctgcatgatg cagaggttga tccattgacg
     9841 aaggattatt ctgttattac cgctaaggta gaggtagcgg agcttatggg ctccgaaacc
     9901 tacctgcatt ttacaaaatg cggcgtacag gcaattgcca gagttgacgc aaagacctcc
     9961 atgagggcag gggatactgc cgaggtgcat ctgcattggg ataagataca tctgtttgac
    10021 atagagactg aaaaggcaat ataaaaatat atataaatat gataaactat aagcggaggg
    10081 ttttaccctt cgcttatagt tctatcagag tcaagtcgag gtatgcatat ggctgatctg
    10141 caaaggctgc ttgcacaggc ggcaaggata actgaaaagc gtatggctgt tattacaccg
    10201 aatgaaaggt tggcctggcc ggaagattcc gacatagata taacctttga agaagcatgc
    10261 aaaaattcgg ggaaaaactt caggggctgg aatatataca tgccggaaga tgcgggaacc
    10321 aggtttttcc tttgcatcag cgcggacagt tactatgaat cggaggtcgg caaactggta
    10381 atgctgcttc taaattcagc ctttgaaaag gatgattccg catcggctta tctgaaaagg
    10441 gctttggaag gcaatctgga tgccgccggg ctttcaatgc ttgaggaaaa cttgaaaagc
    10501 tgcctcccgg ggtatgtact gctgattgac aatattgaaa actcaaggga tgagattatt
    10561 gagatcctgg aaaatacaat ggatattaaa acagaactta tatatgaaaa caggataatc
    10621 gccgtggcgg aagccgaaaa tatcacagag tcatgcagca gctttataaa aaatgtatta
    10681 agcgaactgc ttattgaatg caatgcagtc ataggaggaa gggccgcaaa agcttcggag
    10741 ctttaccccc tgtacaggaa ctgcatggag gcgctgtccc tcaaacatat atacggactt
    10801 ggggacaatg tccttgatta tgacggcatg tatgggtaca gggtggcata caatctggat
    10861 ccaaggctta aggaattttt gagaagaaaa gtgtttacgg ctgaattcag tgatatggta
    10921 aaaggagaac tggggattac catagaggag tttttcagga acaatctcaa cctgacagac
    10981 acggcggcca agctgtatat acacaggaac acactgcttt acaggcttga aaagatatac
    11041 aagtgtacag ggtttgacct taagaggttt gaggacagct ggcttttcag gctcgcatgg
    11101 atagtatcca gggagagata gaagaggctt cggaggaggc cttttttgtt gttgacatgc
    11161 agtagtatta gtattaatat tattacgaac acatgttcta atgggtacgt agaagtatta
    11221 ttatgtaaac ataatacttc tgtgatataa ttcttatata tattttgcaa gaaggtgtta
    11281 ttatggacag attcaagctt gtatcggact ataagcccac gggggaccag ccggaggcaa
    11341 tagaaaagct ggcgaaagga atactggcag gagaaaagta tcagacgctg cttggggtaa
    11401 caggctcagg aaagaccttt acagttgcca atgtgattga aaaggttcag aagccgaccc
    11461 tggttcttgc ccacaataag actctggctg cacaactctg cagcgagttc aaggagtttt
    11521 tcccccacaa ctgtgtagag tattttgtca gctattacga tttttaccag ccggaggctt
    11581 atgttgctca ttccgataca tatatagaaa aggaagctga tataaatgat gagattgata
    11641 agctcagaca ctcggcaaca gctgcgctgc ttgagagaaa agatgtcatt atcgtagcca
    11701 gcgtatcctg catatacggc cttggggatc ctgaggacta caggtctctt gtgctgtcct
    11761 tgagaaaggg aatggtcagg gacagggatg aggtgctgag aaagcttatt gatatccaat
    11821 atgacagaaa tgatataaat tttgtcagag gcaccttcag ggtaaggggg gatattgttg
    11881 atattttccc tgccgcctcg tcggataagg cgataagagt tgaatatttc ggggatgaaa
    11941 tagaaagaat tctggagata gactctctta ccggaaatat tacgggagaa cggcagcatg
    12001 tatatattta ccctgcttcc cacttcgcca ctacccaggc caagcttgag gctgcaataa
    12061 gcaatattga ggcggagctt atagagaggg taaaggagct gaaggagcag gataagctcc
    12121 ttgaagccca gaggcttctg cagaggacca attatgatat tgagatgatg aaggaggtag
    12181 gctactgctc gggaatagag aattattcga ggcacataag cggcagggcg ccgggaagct
    12241 cacccttcac cctgctggac ttctttccga aggattatct gttgatagtt gatgaatccc
    12301 atgtcacaat accacaggtc aggggcatgt atttcgggga caggtcaagg aaggagtccc
    12361 ttgtggatta cggcttcagg ctgccgtcgg catttgacaa caggcccttg aattttgaag
    12421 agtttgagca gaggcttaac caggtgattt atgtcagtgc cacaccggcg aagttcgagc
    12481 ttgagcgaag cacctgcgtg gctgaacaga ttataaggcc taccggattg gtggaccccg
    12541 agatagaaat ccggccggta aaggggcaga tagaccacct gatcggtgag gtgagggaga
    12601 gggctgcgaa taaccagaga gtgctggtta ccaccctcac caagaagatg gctgaggatc
    12661 tgactgctta tatgaaggaa ctggatatca gggtgaggta tctgcattcg gacattctca
    12721 ccttagagag aatgaagata ataagaagcc tcaggctggg tgaatttgac gttcttgtgg
    12781 gcataaacct gctcagggag gggttggacc ttcccgaagt atcccttgtt gccatacttg
    12841 atgccgataa ggaaggattc ctaaggtcgg agatatccct tatccagacg gtggggaggg
    12901 ctgcaaggaa tgtggacgga aaggtaataa tgtatgccga caatattacc gattccatga
    12961 gaagggccat aagtgagacc aacagaagaa gaaagattca gatggattat aacgaaaagc
    13021 acgatataaa gccccagact atcagaaagg cgatacacga aatcattgaa gccactactg
    13081 ccgcagaaga gtccgcaaaa tacggcatta agcatgatat tgaattcatg aggaaggatg
    13141 atttgctcag actgattgcc gctttggagg aagaaatgaa ggactgtgcg agagcattgc
    13201 agtatgaacg ggctgcggaa cttagggata aaataattga attaaggcat aggctgaaga
    13261 aataacgagg tgtataatgg caaaggataa aattattatt aagggtgccc gggagcacaa
    13321 ccttaagaat atagatgttg aaataccaag agataaattt gtagtaatta cagggctaag
    13381 cggttccggt aaatcctccc tggcttttga taccatatat gcggaggggc agaggcgcta
    13441 tgtagagtct ctttctgctt atgcaaggca gttccttggt cagatggaaa agccggatct
    13501 ggattatatt gagggcctct ctcccgcaat ttccatagat cagaagacaa ccaacaggaa
    13561 tccccggtca acggtgggca ctgtaacgga gatatatgac tatttgaggc tgctttatgc
    13621 cagaataggg acaccccact gccatgtgtg cggcaaggaa atatcccagc agacggtgga
    13681 tcagatggtt gactatataa atacccttga accgggaacc aggatacagc ttcttgcccc
    13741 ggttattcag ggcaaaaagg gagaacatca gaagctcctg gaggaaataa agaaaagcgg
    13801 ctacgtcaga gcaagaattg acggggagat tgccgacctc aatgaggaaa tcaagctgga
    13861 aaagaacaag aaacatacaa tagaagtaat cgttgacaga atcatagtca aggaaggcgt
    13921 aaacaagagg ctgacggatt ccctggaaac cgtgctgaag ctttccgggg gaacggtgct
    13981 tatcgatata atcggcaagg aggaacgtct gttcagccag aactttgcct gtgtggagtg
    14041 cggaataagc atagaagatc ccgcacccag gatgttctcc ttcaacagcc cctttgggaa
    14101 atgcccggtt tgcgacggcc ttggtgaact tcggaggata gacccggatt tggttatacc
    14161 ggataagaca aagtcggtcc tggaaggggc aatagagccg tggaagaatc ttgatgagac
    14221 ttcatggtat tacaatgtag tcaatgcggc cgcaaaacac tacggtttca gtatgaatac
    14281 ccccatatgc gaattggatg aaaaagctgt acagattctt ttatacggca acggaggaga
    14341 gaagattaag gtaaagtatc agagagagta cggcagcggc gaagtgatga tggctttcga
    14401 gggtattgtg aacaaccttg aaaggcgtta tttggagaca aagtccaact acatgaggta
    14461 tgaaatagaa aatttcatgt cctctgtacc atgccccgag tgcaaggggg caaggcttag
    14521 gaaggaaagc cttgcggtta ctttaggcgg attgtccatt gctgaattat gcagcctgtc
    14581 tgtatataag ataatggagt ttctcgggag cctgaagctt accgaaaagc agcggattat
    14641 atcaaagctg gtgcttaaag agctgcacga gagattggaa ttcctggtta ctgtcggcct
    14701 tgaatacctt actctttccc gtaccgcagg aaccttatcc gggggagaag cacagaggat
    14761 aaggctggct acccagatag gctccagcct tgtaggagta ttatacatac ttgacgagcc
    14821 cagcatagga ctgcatcaga gggacaacgg caagctcctg aaggcccttc ggaacctcac
    14881 ggatttaggg aatacgctgc tggttgttga gcatgatgag gagacaatgc atgcagcgga
    14941 ctacatcatt gatatgggcc cgggagcagg agcccacgga gggaagatag ttgcagccgg
    15001 ctcctttgat accatactgg cttgtgaaga atcagttacg ggccagtatc tgagcggaaa
    15061 aaaacagatt cccattccgc aggagcgaag aaaacccaat gggaaatgga ttgaaataaa
    15121 aggggcaagg gagaataacc ttaaagactt ggatgctaat atacctctcg gggtgtttac
    15181 ctgcataaca ggagtctccg gctcaggaaa aagcacctta atcaatgaaa tactgtataa
    15241 ggagctttca cacagattgt acggttccaa gctgagggct ggagagcata aatggatcaa
    15301 agggatagag catattgaca aggttattga cattgaccag tcacccatag gaagaacgcc
    15361 caggtccaac ccggctactt atacaggggc tttcgatatt atacggggag tgtttgcagc
    15421 cacgccggag gcgaaaatga agggctacaa taacggcagg ttcagcttca atgtaagggg
    15481 cggaagatgt gaggcctgca agggtgacgg tataatcaaa attgaaatgc agtttcttcc
    15541 ggatgtgtat gttccttgtg aagtatgcaa ggggaagaga tacaacaggg agaccctgga
    15601 ggtacgctac aagggcaaaa acatttctga agtacttaat atgactgtgg aggatgctct
    15661 tgaatttttc aagaacatac cgagagtcca gaaaaagctg cagacattat ttgatgtggg
    15721 gctgggatat ataaaactgg gacagccttc taccgagctg tccggcggtg aggctcagag
    15781 gataaagctg gcttacgagc tgtcaaagag aagcacagga aagactgtat atatactgga
    15841 tgagcctacc acagggcttc atattgccga catacacaag ctgcttggca tacttgacag
    15901 gcttgtagat tcaggcaaca gcattatcgt aattgaacac aacctggatg taattaaaag
    15961 ggcggattat ataatagact tggggcctga agggggagac aggggaggca ctgttattgc
    16021 ccaaggcacc cctgaggaga tatgcaaggt tgaaacctca tacacgggcc agtttttgaa
    16081 gaagatactg gctaaatatt aggaggacac ggg
//
LOCUS       DAOQUR010000050        15862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4200_length_15862_cov_10.035048, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000050 DAOQUR010000000
VERSION     DAOQUR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15862
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4200_length_15862_cov_10.035048"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..537)
                     /locus_tag="PKI50_09605"
     CDS             complement(37..537)
                     /locus_tag="PKI50_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNT03996.1"
                     /translation="MLVLMYMTGNFQNLYQNGKQIVSSAATTPDAATQPEVSQGGLID
                     SAVVRGKTVLNISNMPIGAILEEMGGTYEWNAAEQKATIHWNGMTVELWNGQKSIKVN
                     GVEKEVDIAPNVGTTASVILPSGFAANEPDRILSWEEGSIQIWNSVQKDIQNPSDKKM
                     SRAFKK"
     gene            complement(549..752)
                     /locus_tag="PKI50_09610"
                     /pseudo
     CDS             complement(549..752)
                     /locus_tag="PKI50_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
     gene            1014..3200
                     /locus_tag="PKI50_09615"
     CDS             1014..3200
                     /locus_tag="PKI50_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNT03997.1"
                     /translation="MGKTLVLAEKPSVGRDLAKVLNCNQNSNGYLVGQKYIVTWALGH
                     LVTLADPEAYNKKYKNWNLEDLPMLPDRMELVVIKETSKQYGIVRSLMKQQDIDELVI
                     ATDAGREGELVARWIIEKAGFRKPIKRLWISSQTDKAIKEGFNQLKPAKEFDNLYKAA
                     QCRAEADWLIGLNVTRALTCKYNAQLSAGRVQTPTLAMIVQRENEIKSFVPKDYWTIE
                     AKVNGFTLLWKDKSGNARLFDKSKAEAIVSKLTGQTVEITDIKKEAKSEPHPLAYDLT
                     ELQRDANKRYGYSAKQTLSIMQSLYERHKVLTYPRTDSRHITADIVPTLPDRLKSIAI
                     GPYAAIAQNLLRGKISTTSRLVDNSKVSDHHAIIPTEQPVRLSALSNEERNIFDLVAK
                     RFLAVLSPAYQYEQTTVKAESSGEQLIARGKVTKSLGWKAVYNDRSNTDSANDDEDKE
                     QSLPPMQKGDKLKLLSVKSVNRKTKPPARYTEATLLTAMEHPGKFIEDESLREALDRT
                     NGLGTPATRADIIEKLFNTFYIERKGKEIYPTAKGIQLIDLAPSELRSAELTAKWEQQ
                     LYDISKGKANADVFISNIRSHAGKLVSSVAASAEAYRHDNMTRTRCPECGKFLLEVNG
                     KKGRMLICPDRECGYRKGVAQLSNARCPECHKRMELKGEGEGRLFTCSCGFREKLSSF
                     SKRMDEKTESSDKRTVQKYLQQQKKEEPINSAMAEALVKWKEMHDK"
     gene            complement(3240..>6110)
                     /locus_tag="PKI50_09620"
     CDS             complement(3240..>6110)
                     /locus_tag="PKI50_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT03998.1"
                     /translation="DAEINTLVKMGVDIKCNVKLGEDITLAYLKKTYDAVFIAIGAWA
                     SMNMGCIGEDMEGVMGGIEFLRKATLAEPIYLGQSVLVIGGGNTAMDVARTAVRLGAE
                     HVQVLYRRTRAEMPAEDIEIEEAEEEGVEFTYLVAPIEIIEGNGRVKAVRCQRMKLGA
                     PDKSGRRSPEPIPGDEVTIPADLVISAIGQRVNAESVKELELTKKGAIAANANTFETN
                     IPGVFAGGEAVTGPKIAIEAIAQGKNAASVIDSYLKGEIKPVANPCYIEQEDLGPEDF
                     ADKEKKEREHQKVYPAKIRKMSFGAISETFDEETAVKEASRCLECGCHDYFECKLVNY
                     IGKYDIDTKAIKGEIHRKEEADTHPFIKRNAEKCILCGLCFRACEEVIGVTALGLLDR
                     GFDSVVVPEFGLPLEESACISCGHCIDVCPTGACMEKQAINKQVPVELEDTASVCSYC
                     GVGCNVVYQSRGSLVFKSRPNKAKGEDTLCAKGRFGIHHINDGNRLLKPIIRRSGDTV
                     EAEFDDAIALAAKKLQLIKGQYGKNSVAVFMSPKFTNEEAYLTKKISEKLDTGIIGTL
                     SAKEESGIKGILGCNASANSYDELYNTDLIITVGDVAENHPVLGIKIKDAAKRSKLVS
                     ISNSDTRLAEHADVALKIENNIKFLKSFVKSLIDQKFADKDKADKISIGYDELAKYVD
                     NAPNDEDASKAAKMYGEAKKAIIVIDEETVSEEAIKLIADAAVITEKIGKPHRGIIIA
                     RSKANTQGIIDMGFTVPANKLIESIEKGEIKGAVIIGEDPAASDERIKELLEKLEFTV
                     CLDLYITETAKLADVVIPVCSAAESEGTYTRSDRKVQRLEAAVKPLTGKTTFDVLSRL
                     GAQMGVMIDTVKEAEEKISNEVKQYSGLFAALTEGIEPYVPCSRGNVYGSQVLYTEGF
                     MTEDRKANLSIPAGEQMFKERTVCDTVQLRFEEYIKEKEIKA"
     assembly_gap    6111..6210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6211..6680)
                     /locus_tag="PKI50_09625"
     CDS             complement(<6211..6680)
                     /locus_tag="PKI50_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT03999.1"
                     /translation="MAPVRVNINNRELTADEGSTILRIALDNGIGIPNLCYDENLKTY
                     GACGMCVVEVEGNPKLVRACSTPAVNGMIIKTDTPRTIAARKTALTLLSSDHKGDCRP
                     PCVNACPGHTDCQGYVGLVANRQYDEAIKLVKEVIPLPASIGRVCPHPCETACR"
     gene            complement(6697..8454)
                     /locus_tag="PKI50_09630"
     CDS             complement(6697..8454)
                     /locus_tag="PKI50_09630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNT04000.1"
                     /translation="MKLRIGLGSCGIAAGGKKVREALLNCLKEKDMNIEITSTGCIGM
                     CFYEPIVDVIDDEGEVYTYVNVTPEKAEEIVEQHIKNGVPVEKYIQSTGKKPFKQLEK
                     QVRVVLQNCGIINPEKIEDYLEKGGYKALEKCLKEMTPDQVIEEIKKSGLRGRGGAGF
                     PTWFKWDAARKAKGNEKYVVCNADEGDPGAFMDRSVLEGDPHGLLEGMIIAGYAIGAN
                     HGFIYCRAEYPLAIERLEGAIAQAHEANFLGGNIMGSGFSFDIKIKKGAGAFVCGEET
                     ALIASLEGERGMPRLKPPFPAQSGYFGKPTNINNVETYANVPWIIRNGGAAFAAYGTE
                     KSNGTKVFALAGKIKNGGLVEVPMGMPIREVIYEIGGGIKNDRDFKAVQMGGPSGGCI
                     PKSLIDTPVDYESITRTGAIMGSGGMVVMDEATCMVDMARFFLNFTCDESCGKCIYCR
                     IGTKRMLEILERITKGEGRMEDMDELEQLCISIKDGSLCGLGQTAPNPVLTTLKYFKD
                     EYIAHIRDKKCPAKQCKSLITYSILKDKCTGCTACARKCPTEAITGERKQPHSIEQEK
                     CIKCGNCAITCKFGAINVE"
     gene            complement(8451..8981)
                     /gene="nuoE"
                     /locus_tag="PKI50_09635"
     CDS             complement(8451..8981)
                     /gene="nuoE"
                     /locus_tag="PKI50_09635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT04001.1"
                     /translation="MSCTCEKKLETNFCKKEVDLSLMEPVFKKYEGQSASLISILQDT
                     QGIYGYLPLEALKTIADRTGNKKAKIYGIATFYSQFRLNPIGKHIILQCQGTACHVNG
                     SDKISEAICDELGIEPGGTSKDGMFTLENVACLGCCSLAPVMMINGEAYGNLTPNSAR
                     EVIRGIYKKEKGGASN"
     gene            complement(9073..9987)
                     /locus_tag="PKI50_09640"
     CDS             complement(9073..9987)
                     /locus_tag="PKI50_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT04002.1"
                     /translation="MSNKTLGIIYAVSSAVSFGFVPVFAVIAYNAGTNAVTVLLLRFL
                     IASLILAGILRCRKVELKVGKKLFCRLAYIGIIGYAATTITVFLSYNYISIGLAMTLH
                     FIYPAVVAVLSFFIFKEKMPALKMIALAISIVGVYILAGNTGSSVDLKGVALALASGI
                     LYSIYTIEIGKEELMAMDGLLITFYVSFFSAAAFFIFGAASRNLMFSMQLPAVLGILG
                     LALISTVFGILAYNKSIQMIGPTYAAIFSTSAPITSVVMGIILFSEKLSLTSAIGSIM
                     VIASVLLFSCDKNAKTKDDKVLDTSHSI"
     gene            complement(10140..10349)
                     /locus_tag="PKI50_09645"
     CDS             complement(10140..10349)
                     /locus_tag="PKI50_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HNT04003.1"
                     /translation="MDRLALILVIVGALNWLLVGLFQYDLVASLFGGQDELLSRLIYT
                     LVGVAGIWSISLLFRERERAAGNEK"
     gene            10603..11148
                     /locus_tag="PKI50_09650"
     CDS             10603..11148
                     /locus_tag="PKI50_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04004.1"
                     /translation="MKNIKYIQIIKGFIALIITVALLYTGQALWRSYTIDLPLDKTLN
                     EIDGVEEVSWDAGSSFNDAVIINIKLGNIDNLQKTYKEIKEKIDLTLNGKKYSLEIAD
                     DRSQELEQAYYDIHYYVQKSIIDGDFPSLEAEVREKAEMIGAEAKVYVDEQNIYLQFT
                     KKDNSLFAAIGRHFDRTGGNL"
     gene            11148..12629
                     /locus_tag="PKI50_09655"
     CDS             11148..12629
                     /locus_tag="PKI50_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT04005.1"
                     /translation="MKNREIFAGAAAGALIFLALQRVNILPVVFILGLIGIVYYVNFG
                     RRSGNSISFASAERNICSVSFDDIGGQEVAKNELREALEFMKNDEQIRLLGIRPLKGI
                     LLSGPPGTGKTLLAKAAARFTDSVFLSASGSEFVEMYVGVGAQRVRSLFEQARKAAEY
                     LKKSSAVIFIDEIEVLGGARGQNNGHQEHDQTLNELLVQMDGISSDDRIKILVIGATN
                     RIDMLDPALLRPGRFDRLVKVDLPDKKGRMHILELHVRNKPLASDVNVEALAKDTFGF
                     SGAQLESVANEAAILAMRDGSDKIDSNHFRSAVEKVMMGEKLDRLPSKEEKRRIAVHE
                     SGHALVGELRLPGSVASVNVASRSNALGYVLQTQENDMYLYTCDYLKSRISAALAGII
                     AEELIFGNRSTGGSGDFKHASDIAKQIVYSGMSELGIISSEDIPGDQLHSAITSILRD
                     VESSTRDFLSNYRRELKRISELLLDKETISGEELRLILISECA"
     gene            complement(12668..13519)
                     /locus_tag="PKI50_09660"
     CDS             complement(12668..13519)
                     /locus_tag="PKI50_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNT04006.1"
                     /translation="MNFLVDYHVHSIYSSDGHNTIMEMCESAVNNGIGEIAITDHFEP
                     NIADEYGSSYNQKGYWAHINKARQAFKGRLNIKLGVELGQPHLFSEASSAVIADFPYD
                     YIIASVHKLPAGMDIKELDYSRIGEEELCEMYLRQLKQLVYWNNFDCIGHLDLIKRYS
                     APVYKKNISLTCRHELLKGVLLLAVQSGKGIEINTSGLRQSPKEAFPGLDVVKLYRNM
                     GGEILTIGSDAHMAEDVGRGLKEALDMAREAGFRHITVYNRRKPEWVSITEGKNTFGS
                     AKQIINR"
     gene            complement(13673..15340)
                     /locus_tag="PKI50_09665"
     CDS             complement(13673..15340)
                     /locus_tag="PKI50_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNT04007.1"
                     /translation="MPDLNSLIQESITFQIIFNLFGGTALLMFGVDFMGGGLEKASGK
                     VMKRILSVLTKNIYVAFFVGTILTMLVQSSTAITVMTVGFVNAGLMNLTQAVGIIYGT
                     NIGTTITAQLMSLKLTYIALPIIAVGFYILSFSKKDSTKSLGQAIMGFGLMFLGLKTL
                     NASLPYIKDSETAMQLFLQYGQNPFLGLFVGMIATMLIHSSAATVALTISLFSADLIS
                     FYAAVGLTLGDNIGTCVTAQLASLKANTAARRTAWAHTLYNVIGVVIAIIVLRPFSGM
                     IEWITFSILHEPKAFLVANTHTLFNIISALVFLPITKYYVLFLEKIIPDRRDKKYEAG
                     TLDRLLLDTPVAALKAATQEVINAAEVAKNMLNDCLISLIEDNESKIDSVLQNEEKLN
                     IMQTEITQYLVEISHKDLDSYQSARIPALINTINNFERVGDHCEDITELSQSKINRKI
                     IFSEKGIDELETLYQEVMSMMNECCLAYSKDDAELALQAAAREEVVDELTLKFKEKHI
                     RRLEEGTCSVQAGVIYLDAITHLERIADHLHNVCLILSEGRRNADKN"
BASE COUNT         4255 a         3776 c         3156 g         4575 t
ORIGIN      
        1 ttatatatcc cgccccattc tcctatgggg ttgttcttat tttttgaatg ctctgctcat
       61 ttttttatcg gaagggtttt gtatatcctt ttgaactgaa ttccatatct gtatgctgcc
      121 ctcttcccag gaaagaatcc tgtcaggttc attcgctgca aaaccggaag gcagtattac
      181 acttgcagtt gtgcctacat tgggagctat gtctacttcc ttttctacgc cgttcacttt
      241 tattgatttc tgtccgttcc acaattccac agtcattccg ttccaatgga tggttgcctt
      301 ctgttctgct gcgttccatt cataggttcc gcccatttct tccagtatag cgccaatagg
      361 catgttgctt atattaagca cggtcttccc ccttaccaca gctgaatcaa taagccctcc
      421 ctgtgaaacc tccggctgtg ttgcggcatc cggtgttgtg gctgctgatg atactatctg
      481 ttttccgttc tggtacagat tctgaaaatt ccccgtcatg tacatcagta ccagcaagcg
      541 caacgtactc ccataaaatt cattttaagc ccttcgtata cttcaggatc tacatctttt
      601 gcaacgccta ttctgtgttt atccgcccac tgccgtgcac cttcttcttt caaagtaaac
      661 tttgctttag gcgccttaca ctgaggacaa gcctcgggtg cctccggccc ctcatgaaca
      721 tacccgcaaa ttgtacatac aaactttttc atataaatcc cttcataaat atttattctt
      781 tattaataat tattctcctg ttcataatgt taccatacac cttctgtttt agcaagggat
      841 tttatgaaat tttttaacaa ttcatatctg acccttaaag tatcggctgt aatcaatgga
      901 actatcccat acaagtgatt cgctaaaaaa tagtgtaaaa aaagtctata aatgataaaa
      961 taagtagggg cagtcaaaat acccctgctt attcgtctgt aaggagaatt aaaatgggaa
     1021 aaacactggt attagccgaa aagccctcag tagggaggga tctggcaaag gtattgaact
     1081 gcaatcaaaa cagcaacgga taccttgtag ggcagaaata tatagtaacc tgggctctcg
     1141 gccacctggt tactttagct gaccccgaag cctataataa aaagtataag aactggaacc
     1201 ttgaagacct gcccatgcta ccggatagaa tggagcttgt tgttatcaaa gagacctcaa
     1261 agcagtacgg aattgtccgc agccttatga agcagcagga tatcgacgag cttgtcattg
     1321 caaccgacgc agggcgggaa ggagagctgg ttgcacgatg gataatagaa aaagcaggct
     1381 tcagaaagcc tataaaacgt ttatggattt catcacagac cgataaggcc ataaaagagg
     1441 gctttaatca gctgaaacct gccaaagaat ttgacaacct gtacaaagca gcccagtgca
     1501 gggctgaagc ggattggtta atcgggctta atgtcacccg tgccctcact tgcaaataca
     1561 atgcccagct gtctgccggc agagttcaga ccccgacatt ggcaatgatc gtacaaaggg
     1621 aaaatgaaat aaagagcttt gtccccaagg actattggac aattgaagcc aaagtcaacg
     1681 ggtttacatt gctttggaag gataaaagcg gcaatgcccg gctttttgac aaatcaaagg
     1741 cggaagccat tgtctcaaag ctgaccggtc agacagtgga gataactgat ataaagaaag
     1801 aagcgaaaag cgagccccac ccgctggcat acgaccttac cgaattgcag agggatgcca
     1861 acaagcgcta cggctattcc gccaaacaga ccctttcaat aatgcagtcc ttgtatgaga
     1921 ggcataaggt gcttacttat ccaagaacgg attcaaggca cattactgcc gatattgtgc
     1981 cgactttgcc ggacaggctg aagagtatag ccataggccc ctatgctgca atagctcaaa
     2041 acctgcttcg gggcaaaata agcaccacca gccgtctggt ggataacagc aaggtcagcg
     2101 accatcacgc gattatacca acggagcagc ctgtcaggct ctctgccctg agtaatgaag
     2161 aacgcaacat atttgatttg gttgcaaagc gattccttgc agttttgagt cccgcttatc
     2221 agtatgaaca gacaaccgta aaggcagaat catcgggaga gcagctgatt gccagaggta
     2281 aagtgacaaa atccctgggc tggaaggctg tatataatga caggagcaat accgacagtg
     2341 cgaatgatga cgaggacaag gaacaatccc tgcctcccat gcaaaagggc gataagctga
     2401 agctcttatc cgtaaaatcc gtaaacagga aaaccaagcc ccctgcacgg tatacagaag
     2461 ctacacttct tactgccatg gagcacccgg gtaaattcat cgaggatgag tctttgaggg
     2521 aagctctgga cagaaccaat ggattgggta ctccggcaac cagggctgat ataattgaaa
     2581 agctgtttaa cactttctat atcgaaagaa aaggcaagga gatttatccc accgcaaaag
     2641 gaatacagct tatagacctg gctccctcgg agctaaggtc tgccgaactg accgcaaaat
     2701 gggagcagca gctttatgat ataagcaaag ggaaagccaa tgcagatgtt tttatcagta
     2761 atataaggtc ccatgccggt aagttggttt cctcagttgc agcaagtgcc gaggcataca
     2821 ggcacgacaa tatgacaaga acccgctgcc ctgaatgcgg caagttcctg ttggaggtaa
     2881 acggcaagaa aggcagaatg cttatttgcc cggacaggga atgcggctac cgaaagggcg
     2941 ttgcacagct ttccaatgcc cgctgtcctg agtgtcataa gagaatggag ctgaagggag
     3001 aaggcgaagg caggctcttc acctgcagct gcggtttccg cgaaaagctg tccagcttca
     3061 gcaagcgcat ggatgaaaaa accgaaagca gcgataagcg aacggtgcaa aagtacctgc
     3121 agcagcagaa aaaagaagag cctataaaca gcgctatggc tgaggctctg gtcaagtgga
     3181 aggaaatgca tgataaataa taatcgggcg cccttgtaag gtgtccgatt attattcttt
     3241 tatgccttta tttccttttc ctttatatac tcctcaaatc tcaactgtac tgtatcacaa
     3301 actgttcttt ccttgaacat ttgttctcct gcaggaatac tgaggttcgc ctttctgtct
     3361 tccgtcatga agccttcagt gtacagtacc tgggaaccat aaacattacc cctgctgcaa
     3421 ggaacatatg gctcaattcc ctctgtcaaa gcggcaaata atccgctgta ctgcttaacc
     3481 tcattggata tcttctcctc tgcttcctta acggtatcaa tcataacacc catctgtgca
     3541 cccagccttg aaagtacatc aaaggtggtt tttccagtta atggtttaac tgcagcctca
     3601 agtctctgta ccttcctgtc tgaccttgta taggttcctt ccgactctgc tgcgctgcag
     3661 acaggtatga caacatctgc aagcttcgcg gtttccgtaa tgtaaagatc caggcaaact
     3721 gtgaattcca atttttcaag aagctccttg attctttcat ctgaagcagc cggatcttcc
     3781 cctattatta ccgcaccttt tatctctcct ttttctatac tctcaataag cttatttgca
     3841 ggaacagtaa agcccatatc tattatgccc tgggtattgg ccttggaacg ggctataata
     3901 atgcccctat gcggcttccc aatcttttct gtaattacag ctgcgtcagc aattagcttg
     3961 attgcctctt ctgatacggt ctcctcatca atcactatta ttgctttctt ggcttcacca
     4021 tacatttttg ctgcttttga ggcatcttca tcattggggg cattatcaac atactttgct
     4081 agttcatcat agccaatgga tatcttatct gccttatcct tgtccgcaaa tttctgatct
     4141 attaaagatt taacgaagct tttcaggaat ttgatgttat tctcaatctt tagtgcaaca
     4201 tcagcatgct cagccaatct tgtatcggaa ttgctgattg ataccagctt ggacctcttt
     4261 gccgcgtcct taatctttat acccagaaca ggatggtttt ccgcaacatc ccccacagtt
     4321 ataatgaggt ctgtgttata aagctcatca tagctgttgg cggaagcatt gcagcccaat
     4381 attcccttta ttccggactc ttcttttgcc gacaatgttc caattattcc ggtatcaagc
     4441 ttttcggaga ttttctttgt caaatatgct tcttcgtttg taaatttcgg agacatgaac
     4501 actgcaacac tgttcttgcc gtactgcccc tttataagct gcagcttctt ggctgccagc
     4561 gcaattgcat catcaaattc cgcttcaacg gtatcgccgg accttctgat tattggtttc
     4621 agcaatctgt ttccatcatt gatatggtgg attccaaaac ggccctttgc acataatgta
     4681 tcttcccctt ttgccttatt ggggcgcgat ttgaatacaa ggcttcccct cgactgatag
     4741 accacattgc agccaacgcc gcagtagctg cataccgaag ccgtgtcttc aagctcaacg
     4801 ggaacctgtt tgttaattgc ctgcttttcc atgcatgcac cggtggggca aacatctatg
     4861 caatgtccgc aggatatgca ggcactttct tcaagaggca aaccgaattc gggaacaact
     4921 accgaatcaa agcccctgtc caaaagcccg agagctgtta ctcctatgac ctcttcgcag
     4981 gccctgaagc acaaaccgca gagtatgcac ttctccgcat ttctttttat gaaaggatgg
     5041 gtatccgctt cttctttcct gtgtatttca ccctttattg ccttggtgtc aatatcgtac
     5101 ttccctatat aattgacaag cttgcactca aaatagtcat gacagccgca ttccaggcat
     5161 ctggaagcct ccttcacggc ggtttcttca tcaaaggtct ctgaaatagc gccaaagctc
     5221 atttttctta tctttgcagg gtataccttt tggtgttccc tttccttctt ctctttatcg
     5281 gcaaaatctt ccgggcccaa atcttcctgc tcaatgtagc agggatttgc aacaggtttt
     5341 atttctccct tcaggtagct gtcgattact gaagctgcgt ttttaccctg agctatagct
     5401 tctatggcaa tctttggccc tgttacagct tcgccgccgg caaaaactcc cggaatatta
     5461 gtctcaaagg tattggcgtt ggcagcaatt gcgcccttct ttgtaagctc cagctctttt
     5521 acgctttctg catttactct ctggcctatt gcagatatta caagatccgc agggattgtc
     5581 acttcatctc cgggaatagg ctcgggactt cttcttccgg acttgtcggg agcgcccagc
     5641 ttcatcctct ggcatcttac agcctttacc cttccgttcc cttcaataat ctctatgggg
     5701 gcaacaaggt aagtgaattc tacgccttct tcttccgctt cctctatttc tatatcttct
     5761 gcaggcattt cagccctggt tcttcgatag agtacctgca catgctccgc acccagtctt
     5821 actgctgttc tggctacgtc catggcagta tttccgcctc ctatgaccag aacactctgt
     5881 ccgagatata taggctctgc aagggtagct tttctaagga attcaatgcc gcccattacc
     5941 ccttccatat cttcgccgat gcagcccata ttcatgcttg cccaggcgcc tatggcaata
     6001 aacactgcat cgtaggtctt tttaagatat gctagtgtaa tatcctcacc aagctttaca
     6061 ttgcacttta tgtctactcc catttttaca agtgtgttta tttccgcgtc nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cttctgcagg ctgtttcgca aggatggggg
     6241 caaacccttc ctatgcttgc cggcaatgga ataacctctt taaccagctt tatggcttca
     6301 tcatattgac gatttgcgac caggcccaca taaccctggc aatcagtatg ccccggacat
     6361 gcatttacac aaggaggccg gcagtctccc ttgtgatccg aagacagtaa agtaagtgcc
     6421 gtctttcttg cagctatagt tcttggggta tctgtcttta ttatcattcc gttaaccgca
     6481 ggtgttgaac atgcccttac cagctttgga tttccttcca cttccaccac acacattccg
     6541 catgctccgt aagtcttgag attctcatca taacagagat ttggtattcc tatcccgtta
     6601 tccaaagcta ttcttaatat tgtactgcct tcatccgcgg tcaattctct attatttata
     6661 ttaactctaa ccggtgccat tttatcactt cctttactat tctacgttta tcgcaccaaa
     6721 cttgcatgtg atggcgcaat ttccgcattt aatacatttt tcctgttcga tactgtgagg
     6781 ctgcttcctt tcaccggtga tagcctcggt gggacacttt cttgcacatg cagtacagcc
     6841 tgtacactta tccttcagga tgctgtacgt tataaggctc ttgcactgct tggcaggaca
     6901 cttcttatcc ctgatatggg ctatatattc atccttgaaa tatttcaagg tcgtcagcac
     6961 cggattaggg gcagtctgcc ccagtccgca cagagaaccg tccttgatgc tgatacatag
     7021 ctgctccagt tcatccatgt cttccatcct gccctctcct tttgtgattc tctcaagaat
     7081 ctcaagcatt ctctttgtgc cgattctgca gtaaatgcat ttgccgcagg attcgtcgca
     7141 ggtgaagttt aggaaaaacc tagccatatc aaccatacac gtagcttcat ccattacaac
     7201 cattccgccg gagcccatga ttgcacccgt cctggtaatt gactcgtaat caaccggagt
     7261 atcaatcaac gatttcggta tgcatccccc tgaggggccg cccatctgaa cagctttgaa
     7321 atccctgtcg ttcttgattc ctccgcctat ttcatatatt acttccctga tcggcattcc
     7381 cataggaacc tcaaccaggc cgccgttctt gatttttccg gcaagagcaa aaacctttgt
     7441 cccgttgctc ttttccgttc cgtatgcggc aaaagccgct ccgccgtttc ttattatcca
     7501 cggtacgttt gcataagttt caacattgtt tatgttagta ggctttccga aataacccga
     7561 ctgagcaggg aaaggaggct taagcctcgg cattcctctc tctccttcaa gggatgcgat
     7621 gagagctgtt tcctctccgc agacaaaggc ccctgcacct ttcttaatct tgatatcgaa
     7681 gctgaagccc gagcccataa tattcccgcc caggaaattt gcctcatggg cttgggctat
     7741 tgccccttca agcctttcta tggcaagagg atactctgcc ctgcaataaa taaagccgtg
     7801 atttgcccca attgcatatc cggcaataat cataccttca agcaaaccgt ggggatctcc
     7861 ctcaagtacg cttctgtcca taaatgcccc cggatctccc tcatcggcat tacaaactac
     7921 atacttttca tttccttttg cttttcttgc ggcatcccat ttgaaccagg tggggaaacc
     7981 ggccccgcct cttcctctaa gccccgattt cttaatctct tctattacct gatcgggagt
     8041 catttccttt aaacatttct caagagcctt atatccgccc ttttccaaat agtcctcaat
     8101 tttctcgggg tttataatac cgcagttctg cagtaccacc ctgacctgtt tttccagctg
     8161 tttaaacggt tttttgccgg ttgattgaat atacttctca acaggaacac cgttttttat
     8221 gtgctgttca acaatttcct ccgccttttc gggtgtgacg tttacatagg tatatacctc
     8281 gccctcatcg tctataacat caactatagg ctcgtagaag cacatcccta tacatccggt
     8341 ggatgttatt tctatattca tatccttttc tttcaagcag tttaacaggg cttccctgac
     8401 tttctttccg ccggctgcaa taccgcagct gccgagacct atccttagct tcaattggaa
     8461 gcaccccctt tttccttttt gtatatacct cttattactt ctcttgcact gttaggtgta
     8521 agatttccat aggcttcccc gtttatcatc ataaccggtg caaggctgca gcatccaagg
     8581 catgccacat tttcaagggt aaacattccg tccttgcttg tccctcccgg ttctattcca
     8641 agttcgtcgc atatggcttc gcttatcttg tcagatccgt ttacatggca agcagttccc
     8701 tgacattgaa gtattatatg cttacctata ggattcaatc tgaattgtga ataaaaggtt
     8761 gcgatgccgt aaatctttgc tttcttgttt ccggttctgt cggcaatggt tttcaaagct
     8821 tcaagaggaa gatatccgta aataccctgt gtatcctgaa gtatgctgat caggctcgca
     8881 ctttgcccct catacttctt gaacacgggt tccataagcg ataaatccac ttccttcttg
     8941 cagaaattag tttccagttt tttttcacaa gtgcatgaca tgagatgtat cccctcctac
     9001 atgtataata atattagata accctaaaat atatataata tatgtgtaaa tttactgaca
     9061 acctcaattt tgtcagatgc tatgtgatgt atccaaaacc ttgtcatcct tcgtttttgc
     9121 attcttatca caggaaaata gcagtaccga agctattacc ataatactgc ctattgccga
     9181 tgtcaaactc agtttttcac taaaaagtat aattcccata accacactgg taatcggagc
     9241 actggtgctg aatattgcgg cgtacgtggg gccaatcatc tgtatggatt tgttgtaggc
     9301 aagtattcca aaaaccgtac taatcaatgc aaggcccaaa atgcccagca ctgcaggcag
     9361 ctgcatcgag aacatgagat tgcgtgatgc agccccgaat ataaagaatg cagcagcaga
     9421 gaaaaatgac acataaaaag tgatgagaag gccgtccatt gccattaatt cctctttgcc
     9481 gatttctata gtatatatgg aatagagaat gccggatgca agtgccaaag caactccctt
     9541 caaatccacc gaagatcctg tgttgcctgc cagtatataa accccgacaa tagaaattgc
     9601 caaagctatc atctttaagg ccggcatttt ttccttaaaa atgaaaaaag aaagaaccgc
     9661 tacaaccgcc ggatatataa aatgcaatgt catggccaaa ccaattgaaa tatagttgta
     9721 agacaaaaat actgttatag tggttgccgc ataaccgatt attcctatat acgcaagcct
     9781 gcaaaaaagc ttcttgccca ccttaagctc tactttcctg cagcgcagga ttcccgccaa
     9841 tatcaaagat gcaataagga atcttaagag caaaacagta acggcattag tccctgcgtt
     9901 ataagcaatt accgcaaata caggcacaaa tccaaaggat acagccgaag aaacggcata
     9961 tataataccc aatgtcttat tactcatgat aaccttcctt tgtatatttt tggcatatca
    10021 gcagtcaata aaatatatat taattataca caactattag cgaattacca atcataaatt
    10081 tataagtgcc ggatacacat gttacttgaa cctgataacc tgtgcacccg gcacttattt
    10141 tacttttcgt ttcctgccgc cctttcacgt tctctgaaaa gcaaactgat tgaccatatc
    10201 ccggcaacac ctactaatgt ataaataagc ctgctcagca gttcatcctg ccctccaaaa
    10261 agggatgcca ccaggtcata ctggaaaagg ccgacaagca gccagttcag tgcgcccaca
    10321 attaccagaa taagtgctaa cctgtccatg tgaataaatc tcctttctat attaataata
    10381 aatatggaaa tatttataac aaaatatatt atgcgcattt ttatgattgc tatacaggta
    10441 ttaaatactt tgcacccatg catattattg ccttggcctg tatcttgccg gctaagcgct
    10501 aggcgcttaa tctttttttg ttcttttgct ctttgtctgg cacataatcc tttttcatga
    10561 tataatgtta tgtaaactat aaacggaaaa aggaggttta ctgtgaagaa cataaaatac
    10621 attcaaataa taaaaggctt tattgcactg attattacag tggccttgtt atatacagga
    10681 caggcattgt ggcggagtta caccattgac ttacccctgg acaaaacgct caatgaaata
    10741 gacggagttg aagaagtttc ctgggacgcc ggcagcagct ttaacgatgc cgtaatcata
    10801 aatattaagc tcggaaatat agacaatctt caaaaaacct acaaagaaat aaaagaaaaa
    10861 attgacctga cactaaatgg caaaaaatat tcacttgaaa tagcggatga ccgatctcag
    10921 gagttggagc aggcatatta tgatatccat tattatgtcc aaaaatccat aatagatgga
    10981 gacttccctt ctcttgaggc cgaagtccgg gaaaaggctg aaatgattgg ggctgaagct
    11041 aaagtttacg tggatgagca aaatatctat cttcagttta caaagaagga caattcttta
    11101 tttgccgcaa ttggacggca ttttgacaga acagggggaa atctttgatg aagaataggg
    11161 agatctttgc gggagcagcg gcaggtgcgc tgatattcct ggcgcttcaa agggtgaata
    11221 tacttcctgt tgtgttcata cttggactca tcggcatagt atactatgtg aacttcggca
    11281 ggcgatccgg caatagtatc agcttcgctt cagcagaaag aaatatatgt tcagtctcct
    11341 ttgatgatat cggaggccag gaggttgcca agaacgagct tcgtgaagct cttgaattca
    11401 tgaaaaatga cgagcaaata aggcttctgg gtattcgtcc cttaaagggc atattgctaa
    11461 gcgggcctcc gggcaccgga aaaacactgc tggcaaaagc agctgcccgg ttcacggatt
    11521 cggtattcct atcagccagc ggctcggaat ttgtagaaat gtatgtcggt gtaggcgctc
    11581 aaagagtgcg cagtcttttt gaacaagcaa ggaaggcggc cgaatacctg aaaaaatcca
    11641 gcgcagtaat ttttattgat gaaattgaag tgcttggagg agcaagaggc cagaataacg
    11701 ggcatcagga gcacgaccag actctgaatg agctgttggt gcaaatggat ggcatatcct
    11761 ccgatgatag aataaagatt cttgtaattg gtgcaacaaa ccgtattgat atgcttgatc
    11821 cggcattgct tcgccccggc aggttcgacc gcctcgtaaa ggttgatctt cctgataaaa
    11881 aaggccgtat gcatatactg gaactgcatg taagaaacaa accccttgca tcggatgtaa
    11941 acgtagaagc cctggccaaa gataccttcg gattctccgg agcgcagctg gaaagcgtcg
    12001 caaatgaggc cgccatactt gcaatgagag acggctccga taagattgac tcaaatcatt
    12061 tcagaagtgc cgttgaaaaa gttatgatgg gggaaaagct tgacaggctt ccaagcaagg
    12121 aagaaaagcg cagaatagca gtacacgaat caggccatgc actggttggt gagcttaggc
    12181 ttcccggttc cgtcgccagt gtcaatgttg catccagaag caacgctttg ggttatgtac
    12241 tccagaccca ggaaaacgat atgtatttgt atacctgtga ttatctgaaa tcaagaattt
    12301 cggcagcact ggccggtata atcgcggaag agctgatatt cggcaataga agcaccggcg
    12361 gttccggtga ttttaagcat gcctccgaca tagcaaaaca gattgtatac agcggaatgt
    12421 ccgagcttgg aatcatctct tctgaggata ttccgggaga ccagctgcat tcagctatta
    12481 catccatact tagagatgtt gaaagcagca cccgcgactt tctttcgaat tatagaagag
    12541 agcttaagcg gatatcagaa ttgctgctgg ataaagaaac aatatcggga gaagaattga
    12601 ggcttatatt aatttcagag tgtgcataga acaaaaacta aaaaaaacca ccttcaggca
    12661 gcattgctta tctgttgata atctgctttg cgctgccgaa ggtgtttttc ccttctgtta
    12721 tacttaccca ttcaggcttc ctccggttgt ataccgtaat atgcctgaag cctgcttccc
    12781 ttgccatgtc taatgcttct ttcaggcctc tgcctacatc ctctgccatg tgtgcatcag
    12841 aaccaatagt cagtatctct ccgcccatat tccggtaaag cttaacaaca tccaggccgg
    12901 gaaatgcttc ttttgggctc tgcctcaatc cggaagtatt aatctcaatt cccttgccgc
    12961 tttgaacagc caacagcagt actcccttca acagctcatg ccggcacgtc aatgagatgt
    13021 tctttttgta tacgggggca ctgtaccttt taatcaggtc caaatgtccg atacaatcaa
    13081 agttgttcca gtaaacaagc tgcttaagct gcctgagata catttcgcat agttcttctt
    13141 cacctattct gctgtagtca agctctttaa tgtccatccc tgcaggaagc ttgtgtacag
    13201 atgcaatgat ataatcgtag ggaaaatctg caattactgc acttgatgct tccgaaaaca
    13261 aatgcggctg gccaagctca actccaagct ttatattgag tcttcccttg aatgcctgcc
    13321 tggctttatt aatatgagcc caatacccct tttggttgta tgacgagcca tattcatccg
    13381 caatatttgg ttcaaagtga tctgttattg caatttcgcc gattccattg tttactgcac
    13441 tctcacacat ttccattatt gtattgtgcc cgtcggatga atagatggaa tgtacatggt
    13501 aatccaccaa aaaattcatt ttctcacctc ctttcccaat ctatgatatc acataattgt
    13561 agtttttgta ttaggaatct atgaaaatta tgttaattat ttgttacaat gaatacaaaa
    13621 aaaaatccat atgcttagca ccggatttag tatatatgaa gctcagctgc agctagtttt
    13681 tatctgcatt acgcctgcct tcagatagaa taaggcacac attatggaga tggtccgcaa
    13741 ttctttccaa atgagttatg gcatccaggt agataacccc ggcctgaaca ctgcaagtac
    13801 cttcctccag tcttctgatg tgcttttctt taaacttcag cgtcaattca tcaacaacct
    13861 cttcccttgc agccgcttga agtgctaatt ccgcatcatc ctttgagtat gccaggcagc
    13921 attcattcat catgctcatg acctcttgat acagtgtttc cagttcatcg atgcctttct
    13981 cactgaatat tatctttcga tttattttac tttgggataa ctctgtaatg tcctcacaat
    14041 ggtctcctac tctctcaaag ttattgatag tgtttataag agcaggtatt cttgcagact
    14101 ggtaactgtc aaggtcctta tgggatattt ccaccagata ttgagttatt tccgtttgca
    14161 tgatatttaa cttttcttcg ttctgcagca ctgaatcaat tttactctca ttatcctcta
    14221 taaggctgat caggcaatca ttgagcatat tcttcgcaac ctctgccgca tttattactt
    14281 cctgagttgc agctttcaaa gcggctacgg gagtatctaa caggagccgg tccaaagtac
    14341 ccgcttcata cttcttatcc cttctgtcag gtatgatttt ttccaggaac agcacatagt
    14401 attttgtaat cggaagaaat accaatgcac tgataatatt gaacaaggta tgtgtatttg
    14461 caacaagaaa agcttttggc tcatggagta tactgaaggt aatccattct atcatccccg
    14521 agaagggtct cagaacaata atggcaatta cgacacctat aacattgtat aaagtatgtg
    14581 cccatgcagt tcttctggcg gctgtatttg ccttgaggct tgccagctgc gccgttacgc
    14641 atgtaccgat attatctcca agagttaatc ccacggcagc gtaaaagctt atcaggtctg
    14701 cgctgaaaag tgatatggtc aaagcaactg ttgcggcact gctgtgaata agcattgtcg
    14761 ctatcatgcc cacaaacagc cccaggaaag ggttttgccc gtattgaagg aacagctgca
    14821 ttgcggtctc gctgtccttt atgtagggca aactggcatt caatgtctta agccccagga
    14881 acatgaggcc gaaacccata attgcctgtc ccaggctttt tgtactatcc ttctttgaga
    14941 aagacaatat ataaaaacct actgcaatta taggaagagc tatataggta agcttaaggg
    15001 acatcagctg ggcagttata gtagtaccaa tatttgtacc gtatatgata cccacagctt
    15061 gagtaagatt catcaatcct gcatttacaa acccgacagt cataacggta attgccgtgc
    15121 tgctctgtac aagcattgtc aatattgtgc ccacaaaaaa agctacataa atgttctttg
    15181 taagtactga gagtattctt ttcataacct ttccggaagc cttttccaat ccgccgccca
    15241 tgaagtcgac accaaacatg agcaaggcgg tcccaccgaa aaggttgaaa attatctgaa
    15301 acgttatact ctcctgtatc aagctattca agtccggcat aggaatcctc catatttgct
    15361 gattaggtat ccattagtat tatatatcat ctaacaatat aataatatca ctatttatgt
    15421 ttttttgtcg atatgcctta taaaaaacgc gtcgaaccct atgcgggaca cggggacgtt
    15481 tctgcgggac acggggacgt ccccgtgtcc cgcgaaaaga aacaccgcat ttgaaatgcg
    15541 gtttcagttt gcagacaaag ctcaaaatag gaacagcaat gtatagtcaa agcatactta
    15601 tacagaaatt gttaatggct cttatgaggc tcaacttaga aacaaccgta tgcgaggcat
    15661 ggaggccgag caagctgtca ttgtcacgga cgacctttga cagcgttagg tttttcttag
    15721 ttgagccgaa ttagagccat caatttttgt atacctatgc gagactatac attgctgttc
    15781 ttatatcgaa ggtttatcat cagtctcacg ccgcatttta ttgcggtgtt gtatcattcc
    15841 gaaaagccgg ctctcctatt at
//
LOCUS       DAOQUR010000051        15781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4223_length_15781_cov_11.651469, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000051 DAOQUR010000000
VERSION     DAOQUR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15781
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4223_length_15781_cov_11.651469"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2556)
                     /locus_tag="PKI50_09670"
     CDS             complement(<1..2556)
                     /locus_tag="PKI50_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT04008.1"
                     /translation="MSNLFHVGIDIGSTTVKVVVLNENCDMVYSRYERHYADIKKKLQ
                     QMLYDAFRKLKNSQLTVMITGSGGISIANSLGVSFTQEVVASTKAIKTYYPETDVVIE
                     LGGEDAKITYFADGIEQRMNGTCAGGTGSFIDQMASLLRVDAEGLNELAENYKVIYPI
                     AARCGVFAKTDVQPLLNEGVAKEDIAASVLQAVVIQTISGLACGRPIRGNVAFLGGPL
                     YFLSELRERFIDTLKLKENQVIFPENSQLFVAIGAALSSLNEKPVFFESLIGSLKSHE
                     SASGGESERLERLFSDEKEYEEFRSRHQKSSVIRRELSTFEGECFLGIDAGSTTTKIA
                     LIDDEGAILYTYYSSNEGSPLKSTVKALKELYRLLPKNARIVNSTVTGYGEGLLKAAL
                     SVDIGEIETISHYKAAEFFCPGVDFILDIGGQDMKCLKIKNGVIESIMLNEACSSGCG
                     SFLDTFANSLGMSVEEFAKEALSAENPVDLGSRCTVFMNSKVKQAQKEGATVGDISAG
                     LSYSVIKNALFKVIKVRNLNELGEKIVVQGGTFYNDSVLRSFELLSGREAIRPDIAGI
                     MGAFGSALIARERYQKGMHTSLLGEHELNEFNLETVVSRCGLCSNNCLLTINKFGNGQ
                     SFISGNRCERGTGKIKEQNLLPNLYKYKYERVFRYKPLDSSLARRGTVGIPRVLNIYE
                     NYPFWFTLFTELGFRVELSDRSSNKLYEKGIETIPSESVCYPGKLVHGHIMNLVEKGI
                     KFIFYPSITHELKEQKKADNHFNCPIVVSYPEVIKNNIDVLREKGILFMKPFLPFDDE
                     DRLVDRLHKEFKMFKISKMEIKESVYTAYMEQEAFKRDIQKAGEEALSYIRANG"
     gene            complement(2832..3551)
                     /locus_tag="PKI50_09675"
     CDS             complement(2832..3551)
                     /locus_tag="PKI50_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT04009.1"
                     /translation="MDKKPSFKPYNQGQITFLPPSLEDLIPENHIVRVVDSAIESIDT
                     KPLYEKYEGVGASSYNPVMMLKIIIYAYTQKIFSCRSIAKNCRENIMFMWLSGMNMPD
                     YKTIDRFRIERMKDIIPDIFTEVIALLHSKGYIKLERYFPDAGKDSWAWVKNPRRFRE
                     GLREKCEELLESIDEIERQDTREYGEGDLPELDAGANIDAQSIRDTTDKINEKLDMRL
                     GDRTSRWDGLKKLWRGFSRTK"
     gene            complement(3718..9888)
                     /locus_tag="PKI50_09680"
     CDS             complement(3718..9888)
                     /locus_tag="PKI50_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT04010.1"
                     /translation="MRILKRTIAFALILAFFISSTGFTQLVYAAAEENTLMVHNQFVS
                     YVVDKNTGRFGISTVEGAPRRSGDQDAPLLFEGSKPDTSFTTFRIDGEDYIFGNSYGF
                     LGIKGGMVKAPKTDQMVNTSLWTLGDLEVEQRLTLVSDSGNPDLGNVRVTYRVSNKGK
                     TEKSVGSRLLFDTKLGSNDGSPIIIPGIDKPVEHEISLEGEEVPAFWQSADADISPSV
                     VSYGLISGWENETPDRMTVAHWGGIGSTKWNYQPDSTVKFTSPFNKHNKSDSAVALYW
                     DPEVLAPGETRVYETFYGLGVFATADGHTFMANMSGPDSLELKEDKTGYIQEEFEISL
                     SLDNGLPVSVPMENVRAVLELSGGLELAPGQSKEINLGLLQRDSRANLVWKVKGVFAE
                     TYRIGQATVYLHSDSLYEPQAYGRYVILPGSSGKVPDIQYTGITPQNLYYEDSRKSFN
                     INGTGFGLMKDRSRWELKLIKEGQNPRVYNINHKQINLAGDTTIQVTLGDMLRDISDA
                     GTYTVKLEHKDFQGCSFEKGLNISTDNQYMSRKYGILAVSDEGNKTYKIKVFNNEGDL
                     KKQPDSVKNKLLLIVRGDIQQKEAGIYEAYSRTETPVEMNEVLLYNSTTPISISESNG
                     SVKLAGNGGLSVSGSITFWKWDFEINLKKGSQYTLKPPDDGDLVNKKIEIALTGAAGG
                     LQNILAGFNLKFNTAYFYKDTDGNGLIFGGSMKLSLSGGGDEDENNNNNNNDNIITNE
                     DKKDEKKDPFKIEAKVDTVAMGQKPDNTIGFKGIAAQATVGFPKDFFPPPVDIGAEAT
                     LKVDTFSDPGEVGLALDVDLKVIKVNGEIEFILKPYPFPDKLYLYVGTDVGVDIIPAI
                     PVATLYGVGGGIDNISNLVNWDSSAPPLTIMLTASAEIGRILKMDKVTLSVSWQHAEL
                     KGNIGIKGYDIIKNATLRLRWYNPIGFHASATIEAFSCIEGKVLLNIYKDSFIGMASI
                     RLFVPNGVPVVGGLTIAGAEAGIDTEKLWGKLTVIGVTMGVTYLFGEEKVEFDVGQNG
                     KLPEGLIASLDGAEGLYAINYRDEGTGEVGRMIFGTNMKLVGSSRQPERVYAKDSIYL
                     AAAGEDRRFLFMDMPVVTALDDTTYRLNVQSREAALFEIEYEGTKPNVRVYRPDGTEY
                     MLVEDDINGNMRYQTIPAKDSDSGRDEQKLWISVVGPQPGMWTIVSDKPLTAAQLYDI
                     KMAPEYTSLDAEKIGTGSVKVDWSGKYLDGAKVNLYLLEEGSSEAGRLLVQDIDAAAG
                     TYTVTLPEDVMTGSYVIRAELSKEDYGYTSRTTGPFTVTDLKAPSKPRNLKVTAAGNG
                     CLKVEWEEGTEVRYPAQGYILTVLNDDGSSVQGYPESYVSGRTEAIIGGEVTQQDGTT
                     LRLETGKSYKISAIAHREEDLVEGEDIPRQHYSEAILSDTVFLPAPHPPALKLSLARE
                     GSMLPLNTAESGIDEYYSTGKEAMLSVEADTAVGANIVLNKETVYSFNPAVRHLQQLA
                     LNEGENMVEIRAENSSGDFTDKVIRIICDTIPPLLLINSTEVTQSNGAASALIKGKCE
                     PGSKLTINGGNVLSDENGLFEYTLDMGDNMSMEIFAVAEDPIGNISEYSGTVYNDRLK
                     DIQRVAITPGGATVELGESLELSLNAIDSEGKHLSIKPELVKWSLMADTGTAVLENGA
                     RIKALKPGKAYIMAEYKVTEDYSHTDAVVVNVVESMGNKPITASRRSSSPAGVLERII
                     EAEKGKKDIYTARLQPGTETKLTADDDLTLDIPAGFAVNSADINISRYDNQEELMKKF
                     PGMKLLSPVYDISLNKGASTGTPVSVSFRYEADQAADLRRIAVYRLDEQQGGWDYIGG
                     TPDKEKGIITVRLSSFSKYAVLENSSLRLLDDVDDARWSRDVIYSLVHMNIVEGIKVG
                     EKYYYMPGNYITRAEFIKLLSAGLEAGDTEADSVQLPFADKKEIPDWALSHIKAAYAN
                     GWVNGRIAGDKRLFGPKEQITREEACAILGRMLGDGIKPKRVYFTDRNQVAKYAINYL
                     DILADMGVLSGYGDDTFRPKNFITREEAAAMIDKYLKNAS"
     gene            complement(9888..15662)
                     /locus_tag="PKI50_09685"
     CDS             complement(9888..15662)
                     /locus_tag="PKI50_09685"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNT04011.1"
                     /translation="MRNLTVKGMISVIMALVLLTSQIVPVAGSGTDVRFDNGAHFASK
                     GIKNCYPDKGQWSTNETNSFYIESHPETGDEIKPKNINNSFKYFWTMPLKGADNQAGL
                     KYKVRLQAYGQAFDGDVASIFLDFWAPLGDGRSGSITVERMQHDFSGWNDVSFSGYVP
                     EGATGMQLTLAGEKGISILDDNKCEIYFRNIEVYITDDVAPIPKYVEFGERRELGTPE
                     ERRMKDYYGIGDDVYWDLEFNEPIFINDPGYLAYVRGLNATERNSFLNAQLSPNYPDE
                     KLLLEHVRDNNGRGILQQFGELKMKFKYRNADGRDMEGYAYPVDKSRYNKETYGNDYS
                     KQIKFKYTVRPGDEFRASDIYEMVLEGGVITDNAFNPMPNEYRIISFASGSASGVSNV
                     YRIYTRDFRVETTAPVLLEINGGLPEGTLDRSQTLPLYFKFSEPVYITISDGAFIKHN
                     INKLEFENYQRAGRFSSYDAKLYLNALNRDWTGALDPYRSGTPDAYYASGNGTTMLEF
                     RFDNMENSFDPLEITGSIYSKWKESGSTERVSYGELYVMDAAGNKAPLLPIGGVKLSD
                     SKRYVGDTEPPVITVHTKEAEKEKGGFYVKINVTDEVSGVDYDTLKFGIGYTNADFGG
                     IMRAAKSLAPGRWYHSHELAGMFGIDPGRTSNYRLFVHAKDKKGNMFYGSTHGYIGYG
                     WATDINVTRTDIDISSIETQKEMSVSLETNLGGAAEGRVYLCSADWVREDGVNWRCKG
                     LWYCMTREPVMPEFSENSGIWRYKESHRINTSEDFEGDPRERDGYYYLHIIAADEERK
                     PIGSKTVPQPLLFDFKPPQVNANAEMQADGSMKVTIHVSDNYTPRKDITAKYIVGNSG
                     RQQLPENGEIIIGKDELKPDTYVNVTATDAHQNNRNMLFGPYGNAESKLSASAPYINF
                     YRGSKYTNEDFAELHIFNSVEEFSWSMDGVSWSGWIPLEKGATSFETQGYSPCIPLPD
                     KEGPITFYTKYRDSAGKESDAAASTVIRDVTPPTARVKYSSYYGSNYFFTAELVDISD
                     NLCPSEDIKVIGYVPGYYKEIIARGEPHYFILEDEAGNKGAVKACVEPIPVVIQPVVD
                     DDDDDDEPEPEPPDTESPTIVVTPNGNHSNAMSVSPKIKVSDNKNIARIEYAFTTESN
                     IEDVTDWTEIPMDDYNPNGNTVSFSGVSGTYYLHVKATDDSGNTGEFTSNPYNMVLEY
                     TEPDIVYSGEHEGVMRAFIVSPEPITVSEAVYDLRAGEEYTFRYTYDSDGPDAEDWLI
                     TAAWESEIDPDMLECKGTVTMSPYDEVTSGPVTVTIEANDGYPMEFMPERLDCDWETL
                     EGALNQYLIDNDNINIIEGRVMQSAFPEDITIRTINSEYLNADLLEELASDEYYYLEV
                     DKAEITFDVNGYFLYMAWGNLYTVQVGHIVNEDWVDNVTVAMTEGSRRAYAPGSLLAS
                     AGKYSGMLKASDSSSVDDLTPPSGRVSYTWADPKKGPVTANLKLSDDSRGNVTIINNG
                     GSGRYVFKNNGQFVFEFEDEAGNQGRALAQLTAIPAEGPRVAVSYSSVYPTREAVKVT
                     MTPEPGSELKSGDIQTVQENDSYTFITEVNGSWNFTFVNEYGIEKVVKPSVENIDRIP
                     PKLWLDYIYDSYKNTITAIVRSDELITPAEGSTLSQVYPCTDKERYTMRALDELGNEV
                     VVTVSAAYLDEPGAGDVKINFSTAELSKNPVTITLTSDKEFTVLNNGGSKELTAQQNG
                     QYIFVIRDSLGQIKALRLEVKNIILEAPVITLGYPDYIEINRRGSIDLMNFTAVDSID
                     GDVKDKVKITGAVNTLKPGIYTVTYSVVNSVGNEAVKTLTVKVRSDEEQIVRINGVRY
                     EGEPIPLAASKLQVTAEGFVGQVSLKLAEGFRNASYFKTDGTAGSLDNIPVSKKGWLT
                     LYVYDNERNSKLINVLITDLGGEQ"
BASE COUNT         3636 a         4302 c         3049 g         4794 t
ORIGIN      
        1 tccattagcc ctgatataac tcaaggcttc ttctccggcc ttttgaatat ccctcttaaa
       61 agcttcctgc tccatataag cggtatatac gctttcctta atttccatct tactgatttt
      121 gaacatctta aactctttat gcagtctgtc gacaagcctg tcttcgtcat cgaaaggcag
      181 gaagggcttc ataaacagta tacccttttc cctcagcaca tctatattgt ttttaataac
      241 ctccggatat gaaaccacaa ttgggcaatt aaaatgattg tccgctttct tttgctcttt
      301 cagctcatgg gttatggatg gatagaaaat gaacttaata cccttctcaa caagattcat
      361 gatatgcccg tgaaccagtt tccccggata gcacaccgac tctgagggta ttgtttcaat
      421 tcctttttca taaagcttgt ttgaagacct gtcggataat tccactctga agcccagttc
      481 cgtaaacagg gtaaaccaga aagggtaatt ttcgtatatg ttcagaaccc ttggaatacc
      541 aacagtaccc cttcttgcta atgagctgtc caagggcttg tatctgaata ctctttcata
      601 tttatactta tacaggttcg gcagtaaatt ctgctctttt atcttgccgg ttcctctttc
      661 acatctgttc cctgatataa agctctgccc gtttccaaac ttgtttattg taagtaaaca
      721 gttgtttgag cataacccgc atcttgatac caccgtttca agattgaatt cattgagttc
      781 atgttctccc agcaagcttg tatgcatgcc cttctgatat ctttcccttg caatcagggc
      841 agatccaaaa gcacccatta ttcctgctat atcaggcctt attgcctctc ttcccgatag
      901 gagttcgaag ctccttaaca ccgaatcatt ataaaaggtg cccccctgga caactatttt
      961 ttctcccagt tcgttcaggt ttctaacttt tattacctta aacaatgcat tcttaattac
     1021 cgaataagat aatcctgctg aaatatcccc tactgtcgcg ccttcctttt gtgcctgctt
     1081 tactttagaa ttcatgaata cggtgcacct ggagcccaga tctaccggat tctctgcaga
     1141 cagtgcttcc ttcgcaaatt cttcaacact catgcccaat gagtttgcaa aagtgtccag
     1201 gaatgaaccg cagcctgaag agcaagcttc gttcagcatt atgctctcaa taaccccatt
     1261 ctttattttc aggcatttca tatcctggcc gcctatatcc aatatgaaat caactccggg
     1321 gcagaaaaat tccgccgctt tataatgtga tatcgtttca atttcaccta tgtccacact
     1381 taatgctgcc ttcagcaggc cttccccata tcccgtaaca gttgaattaa ctatcctggc
     1441 attcttcggc aaaagccgat acagctcttt cagtgcttta acggtggact ttaacgggct
     1501 tccctcatta ctgctgtaat aggtgtaaag tatcgctccc tcatcgtcta taagagctat
     1561 ttttgtggtg gtagagcctg cgtcaatccc taagaagcat tcaccttcaa aggttgataa
     1621 ttcacgccgt atgacagagc ttttttgatg cctggatctg aactcttcat actccttctc
     1681 atcagaaaac aggcgttcca atctttctga ttccccgccg gaggcacttt catggctttt
     1741 caggctgcct atcaatgatt cgaaaaatac aggcttttca ttaagagaag ataatgccgc
     1801 gcctattgct acaaagagct gcgaattttc aggaaaaatc acctgattct ctttaagctt
     1861 caacgtatca ataaatcttt ccctcaactc tgaaaggaaa tataacgggc cgcccaaaaa
     1921 agccacattc cccctgatag gccttccgca tgccagtccg cttatagtct gaattacaac
     1981 ggcctgaagt actgaagctg caatatcttc cttggcgacc ccctcattca gaagaggctg
     2041 cacatcggtc tttgcaaaaa ccccgcatct tgccgctatg ggataaataa ccttgtaatt
     2101 ctcagccaac tcgtttagcc cctctgcatc cactctgagc aaagaggcca tttggtctat
     2161 aaaagaacct gtgccccccg cacaagtacc attcattctc tgttctattc cgtcggcgaa
     2221 ataagtaatc tttgcatcct ctccgcccag ttctattaca acatcggttt caggataata
     2281 ggtcttaatt gcttttgtag aagcaacaac ctcctgtgta aaagaaaccc ccaagctgtt
     2341 tgcaatactt attcctccgg aacccgtaat cattacagtc aattgactgt ttttcagctt
     2401 tctaaaggca tcataaagca tctgctgaag ctttttctta atatcagcat aatgtctctc
     2461 atacctgctg taaaccatat cgcagttctc atttaaaaca actactttaa ctgttgtaga
     2521 accgatatca ataccaacat gaaataaatt actcataacc ccacagcatg caagctgtgc
     2581 ccacctccac ggctctgaat tataaccggg caaccggaat aataaatgca aatcatatta
     2641 taagtatctt ctaagtatca tgctgattaa agcttattaa cattttatat gggtacactg
     2701 aataattcaa taggcaatag aatggcaaat gccaaatact atatgttttt ctatatatat
     2761 tcataataat ccacaaatat tgataatgcg ggtattctcc agcctgattt ttgcagattc
     2821 ccttcctagt atcactttgt ccttgagaat cctctccaca gcttttttaa gccatcccat
     2881 ctgctcgtcc tgtctccaag cctcatgtca agcttctcat taattttgtc agttgtgtct
     2941 ctgattgatt gtgcgtcaat attggctccg gcatcgagtt ccggtaaatc cccttcccca
     3001 tattcccggg tgtcctgacg ttcaatttca tctattgatt caaggagttc ctcacacttc
     3061 tccctgaggc cttcgcggaa cctcctggga tttttcaccc atgcccagct gtccttgcct
     3121 gcatcaggaa aatacctttc aagctttata tatcccttac tgtgaagcag agctatgact
     3181 tctgtaaaaa tatccggaat aatatctttc atacgttcga ttcgaaatct gtcaatggtt
     3241 ttataatcgg gcatattcat tccgctaagc cacataaaca ttatattttc cctgcaattc
     3301 ttggcgatgc ttctgcatga aaaaattttc tgggtataag catatattat tattttcagc
     3361 atcataacag gattgtaact gcttgctcca accccttcat atttctcata caaaggtttt
     3421 gtgtcaatgc tttctatcgc gctgtcaact actcttacta tatgattctc gggtatcaaa
     3481 tcctccagac ttggaggaag aaaggtaatc tgcccttgat tgtatggttt gaaggaaggt
     3541 ttcttatcca taatatctcc ctttcattta tattaatctt ctttcctgcc attgactgca
     3601 tttgactaca ttatagcact ttttgaataa aaatcatagc agggctttgc aaaaccaatg
     3661 gttctgcaaa gccctgccct aatctcgaat ctcgtgtttc ttgtctgaag aatgcgtcta
     3721 tgacgcattc ttcagatact tatctatcat tgctgcagct tcctcacggg taatgaagtt
     3781 cttgggcctg aaggtatcgt ccccataacc gcttaatacc cccatatctg caagtatgtc
     3841 cagatagttg attgcgtatt ttgccacctg gttcctgtct gtgaaatata ctctcttagg
     3901 cttaattccg tctcccagca ttctcccgag gatggcacag gcttcttcac gggttatctg
     3961 ctcctttgga ccaaacagcc tcttgtctcc tgcaatcctt ccatttaccc atccgtttgc
     4021 ataggctgcc tttatgtggg aaagagccca atcgggtatc tcctttttat ccgcaaaggg
     4081 cagttggaca ctatcagctt cggtatcccc tgcttccaag cctgctgaaa gcaattttat
     4141 aaactcagct cttgtaatat agttgccagg catgtaatag tatttctcac ctacttttat
     4201 accttctact atattcatat gaacaagaga gtatatcaca tcccttgacc acctggcatc
     4261 gtccacgtcg tccaacagtc tcaaactgct gttttccagc actgcgtact tgctgaagct
     4321 tgacagtctt actgttatga tacccttttc cttgtccgga gtgcctccta tatagtccca
     4381 tccgccctgc tgctcatcaa gcctgtatac tgcaatcctt ctgaggtctg cggcctgatc
     4441 cgcctcatac ctgaagctta cacttacagg agttcccgta ctcgcaccct tgttgaggct
     4501 tatatcataa acgggactta aaagcttcat gcccgggaac tttttcataa gctcttcctg
     4561 attgtcatac ctgctgatat ttatgtcagc agaatttact gcaaaaccgg ccggtatgtc
     4621 aagggtcaga tcgtcatctg cagtgagttt tgtttctgtt cccggctgca gtctggctgt
     4681 gtagatatcc tttttgccct tttcagcctc gattatcctt tcgagtacgc cggcagggct
     4741 tgaagacctt ctgcttgctg ttatgggctt atttcccatg ctttccacta cattgacaac
     4801 cacggcatcg gtgtgagaat aatcttccgt tactttatac tccgccataa tatacgcctt
     4861 accaggcttc agagctttta tcctggctcc gttttcaaga actgcggttc cggtatcggc
     4921 catcaggctc cattttacaa gttccggctt tatgctcagg tgttttccct ccgaatcaat
     4981 tgcattcagg cttagctcca agctttctcc aagttcaacg gtagctcctc cgggggtgat
     5041 cgccaccctc tgtatgtctt tcagcctgtc attatatacc gtaccgctgt attcgctgat
     5101 attccctatg gggtcctccg caactgcgaa aatttccatg ctcatattat cacccatatc
     5161 cagggtatat tcaaataatc cattctcgtc cgataacaca ttgccgccgt taatagtgag
     5221 cttgcttccc ggctcgcatt ttccctttat taatgcggat gcagcgccgt tgctctgcgt
     5281 gacctctgtg gaattaataa gcagcagcgg cggtatggta tcgcatatta tccttattac
     5341 tttatcggtg aagtctccgg agctgttttc agcccttatt tcaaccatgt tctccccttc
     5401 gttcagggca agctgctgca gatgtctcac cgccgggtta aaggagtaca ctgtttcttt
     5461 gttcaaaact atatttgcgc cgacagccgt atccgcctcc accgacagca ttgcttcctt
     5521 ccctgtggaa tagtattcgt ctatacccga ctctgccgta ttaagaggaa gcatgctccc
     5581 ttcccgggcc agcgaaagct ttagtgccgg cggatgcggg gccggaagaa atacagtgtc
     5641 cgacaaaatt gcttcactat aatgctgcct gggtatgtct tctccttcaa ccagatcctc
     5701 ttccctgtgg gcaattgccg aaatcttata gctcttgcct gtttcaagcc tcagggtagt
     5761 tccgtcctgc tgagtgactt ctccgcctat tatcgcctcg gttcttccgg atacgtagga
     5821 ctccggataa ccctgcaccg atgaaccgtc atcattcaga acagtcaata tatagccttg
     5881 ggccgggtat ctgacctcag ttccttcttc ccattccacc ttcaggcagc cgttgcctgc
     5941 cgcggtcacc ttcagattcc tgggcttgct cggagccttg agatcggtta ccgtaaacgg
     6001 ccctgtggtt ctgctggtat acccgtaatc ctccttggaa agctctgccc gtatcacata
     6061 gctgccggtc attacatcct ccggaagcgt cacggtatag gtccctgctg ctgcgtcaat
     6121 atcctgaact aacagtcttc ccgcttcgct gcttccttcc tcaagcaggt aaagatttac
     6181 ctttgccccg tccagatact ttcctgacca atcaacctta acactgccgg ttcctatttt
     6241 ctcagcgtcc aaggacgtat attccggagc catttttata tcgtacagct gtgctgccgt
     6301 aaggggctta tccgatacta tcgtccacat acccggctga ggccccacaa cggatatcca
     6361 cagcttctgc tcgtctcttc cgctgtcgct gtccttcgcg ggaatggtct gatatctcat
     6421 atttccgttt atgtcgtcct ccaccagcat atactctgtc ccatccggcc tgtagaccct
     6481 cacattgggc tttgtgcctt catattctat ttcgaacaga gcggcttccc tgctctggac
     6541 attcaaccgg tatgtggtgt catccagtgc agtgacgacg ggcatatcca tgaataaaaa
     6601 tctcctgtcc tcccctgctg ccgccaaata tatgctgtcc ttcgcataca ccctttccgg
     6661 ctgtcttgaa gaacccacaa gcttcatatt ggtgccgaat atcatcctgc ctacctcgcc
     6721 ggttccttca tccctgtaat ttattgcata aagcccctca gcaccatcca gggatgcaat
     6781 taacccttcc ggcagttttc cattctgacc tacatcaaac tctacctttt cttcgccaaa
     6841 gagatacgtt acccccatgg tcacacctat tactgtaagc ttcccccaca gcttctccgt
     6901 atctatcccc gcttcagctc ccgcaatggt caatccccct actaccggaa ctccattagg
     6961 tacaaagagc cttatcgaag ccatgccaat gaagctgtcc ttgtatatgt tgagcagcac
     7021 ctttccttcg atgcagctga aggcctctat ggtggcagat gcgtggaagc ctatggggtt
     7081 gtaccatctg agcctcaggg tggcattttt tattatatca tagcctttta tccctatatt
     7141 tccctttaat tcggcatgct gccaggagac actaagggtc accttgtcca tctttagtat
     7201 cctgccgatt tccgcagatg ctgtcagcat tatggtaaga ggcggagctg agctatccca
     7261 gttcacaagg ttggaaatat tatctattcc gccgcccact ccatacaggg tagccacggg
     7321 tatcgcgggt attatatcca ctcccacgtc ggttcctaca tataaatata atttatccgg
     7381 gaagggatag ggcttgagta tgaactctat ctctccgttt acctttataa ccttaagatc
     7441 cacatccaga gctaatccca cttcgccggg atcgctgaag gtatccacct tcagggtggc
     7501 ttcagctcct atatccacag gaggcgggaa aaaatccttc gggaagccta cggtggcttg
     7561 ggcggctatt cccttgaacc ctattgtgtt atccggcttc tgtcccatag ccaccgtatc
     7621 caccttggct tctatcttga aaggatcttt cttttcatct ttcttgtctt cattagtgat
     7681 aatattgtcg ttgttgttat tgttattgtt ttcgtcttcg tccccgccac cacttaaaga
     7741 aagcttcatg gagcccccga atatgagacc atttccgtct gtatccttat aaaagtatgc
     7801 ggtattaaac ttcaaattga acccggcaag tatgttttgc agacctcctg ccgcaccggt
     7861 cagagctatt tctatctttt tgtttacaag gtcaccatca tcaggaggtt tcagagtata
     7921 ttggctgcct ttctttaagt tgatctcaaa atcccatttc cagaaggtaa tggaaccgga
     7981 taccgaaagg cctccgtttc ctgccagctt taccgaccca ttgctttcgc ttatgcttat
     8041 cggcgtcgtg ctgttgtaaa gcagtacttc gttcatctcc acaggcgttt cggttctgga
     8101 ataggcctca tatatccccg cttccttctg ctgtatgtct ccccttacaa tcagcagcag
     8161 tttgtttttg acgctatccg gctgcttttt taaatcacct tcattattaa aaaccttaat
     8221 cttgtatgtt ttgttgcctt cgtcactgac cgcaagtata ccgtatttcc ggctcatgta
     8281 ttgattatcc gtagagatat ttaacccctt ctcaaagctg cacccttgga aatccttatg
     8341 ctccagcttc accgtatagg ttccggcatc acttatatct ctcagcatat ctcccagagt
     8401 tacctgtatc gttgtatccc ccgccaggtt tatctgcttg tggtttatat tgtataccct
     8461 tgggttctgt ccttccttta tcagcttcag ctcccacctg ctcctgtctt tcatcagacc
     8521 gaagcccgtt ccgttaatat tgaaggattt ccgcgaatcc tcatagtaca ggttttgggg
     8581 agttattccg gtatattgta tatccggcac tttgcctgag cttcccggca gtatcacata
     8641 tctgccgtat gcctggggct cataaaggga atcggaatgg agatatacag ttgcctggcc
     8701 tatcctgtag gtctcggcaa acaccccctt taccttccag accaggtttg ccctggaatc
     8761 cctctgcagc agtcccaggt ttatctcctt agactgcccc ggggccagtt ccaggccgcc
     8821 tgaaagctcc agcacagccc tgacattctc catgggcacc gaaacaggaa ggccgttgtc
     8881 cagtgacagg gatatctcga attcctcctg tatataccct gttttgtcct ctttaagctc
     8941 aaggctgtcg ggcccgctca tgtttgccat gaaggtatgc ccgtctgctg tggcaaatac
     9001 tcccagacca tagaaggttt catacaccct ggtttccccc ggggccagca cctccggatc
     9061 ccaatacagg gctaccgcac tgtcgctctt attgtgctta ttgaaagggg aagtaaattt
     9121 cacggtgctg tccggctgat aattccattt ggtggagcct atacctcccc aatgagcgac
     9181 ggtcatcctg tccggggttt cgttttccca gccgcttatc aatccgtagg acaccaccga
     9241 agggcttata tccgcatccg ccgactgcca gaaggcgggt acttcctctc cttcaaggct
     9301 tatttcatgc tcaaccggct tgtcaattcc gggtattatt ataggagaac cgtcatttga
     9361 ccccagcttt gtgtcgaaca gcagccttga gcccacgctt ttttctgtct tccccttatt
     9421 ggatacccta taggtaaccc ttacgttacc caaatcagga tttccactgt ctgagaccaa
     9481 ggtcagccgc tgctccacct ccagatcccc gagggtccag agggaagtat ttaccatttg
     9541 gtcggttttc ggagctttca ccatgccgcc ctttattccc agaaaaccgt agctgttccc
     9601 gaatatgtaa tcctctccgt ctatgcggaa agtagtaaag gaggtgtcgg gctttgaccc
     9661 ttcaaaaagc agcggcgcat cctgatcccc gctcctcctt ggagcgcctt caacggtgct
     9721 tattccgaac cttccggtat tcttatccac cacatagctc acaaactgat tatgaaccat
     9781 cagggtattc tcttccgctg ccgcatatac aagctgagta aaaccggtgg atgaaataaa
     9841 gaacgcaaga atcagcgcaa aggcgatggt tcttttcagt attctcatta ctgttcaccc
     9901 cccaaatcgg ttattaatac attaatcagc ttgctgttcc tctcattgtc atatacgtac
     9961 agtgtcagcc agcccttctt gctcacgggt atattatcaa gagaccctgc tgtaccgtcg
    10021 gtcttaaagt aggaggcatt cctgaagccc tctgccaatt tgaggctgac ctgccccaca
    10081 aagccctctg cagtcacctg cagcttgctt gctgcaagag gtataggttc cccttcgtac
    10141 ctaactccgt ttatccggac tatctgttcc tcgtcacttc tgaccttcac agtgagagtt
    10201 tttaccgcct catttcccac actgttcaca acactgtagg ttacggtgta tatcccaggc
    10261 ttcagggtgt ttacagcacc ggttattttt accttgtctt tcacatctcc gtctatactg
    10321 tcaacagccg taaaattcat caggtctatg gaacccctcc ggttaatctc tatataatcc
    10381 gggtatccca gggtaatcac cggagcctcc aggatgatat tcttcacctc taacctcaga
    10441 gcctttatct gtcccaggct gtcccttatt acgaagatgt actgcccgtt ctgctgggct
    10501 gtaagctctt tgctgcctcc gttgttaagg accgtaaact ctttatcgga ggtgagagtg
    10561 attgttaccg gattttttga cagttcggca gtgctgaagt ttatcttgac atcccctgcc
    10621 cccggctcgt caaggtaagc cgccgagact gttacaacca cctcgttccc cagttcgtcc
    10681 agggctctca tggtatatcg ctccttatcc gtgcaagggt acacctggct gagagtgctt
    10741 ccctccgccg gggttatcag ttcatctgac cttactatag cggtaatagt gtttttgtaa
    10801 gaatcataaa tataatccag ccaaagcttc ggaggaatcc tgtctatgtt ctccacagaa
    10861 ggctttacta ctttttctat gccgtactca tttacaaaag tgaaattcca actgccgttt
    10921 acttcggtga tgaaagtata gctgtcattt tcctgcactg tttgaatatc gccgcttttt
    10981 aactctgatc ccggctccgg cgtcatcgtc actttcaccg cttccctggt gggatatacg
    11041 gaactgtaag agaccgccac ccttgggcct tctgccggta tagcggttaa ctgtgcaagg
    11101 gctctgcctt gatttcccgc ttcatcttcg aattcaaaca cgaactggcc gttgttttta
    11161 aacacatatc ttccgcttcc gccgttgttt attatagtaa catttcccct gctgtcgtcc
    11221 gaaagcttca ggttagccgt aacaggccct ttcttgggat cagcccatgt gtagcttacc
    11281 cgtcccgaag gaggggtaag atcatccaca gatgagctgt ccgatgcttt aagcataccg
    11341 gagtatttac ctgcggatgc cagcaatgac cccggggcat atgcccgcct gcttccctcc
    11401 gtcatggcaa ccgtcacatt atccacccag tcttcattta caatgtggcc tacttggaca
    11461 gtgtataaat tgccccaagc catgtataaa aaatatccgt tcacatcaaa agttatctcg
    11521 gctttatcta cttccagata gtaatattca tctgacgcaa gctcttccag caaatcggca
    11581 ttaagatatt cagaattaat tgtccttata gttatatcct ccggaaaggc tgactgcatc
    11641 acacgacctt ctattatatt tatattgtcg ttatctatca aatattggtt cagcgcgcct
    11701 tccagagttt cccaatcgca atcaagtctt tcgggcatga actccatagg atagccatcg
    11761 tttgcctcaa tagtcactgt tacaggcccc gaagtcactt catcgtaggg cgacattgtt
    11821 actgtgcctt tacattcaag catatctggg tcaatttcag actcccaggc ggcggttatc
    11881 aaccaatcct ccgcatcggg tccgtcggag tcataggtat agcggaaggt atactcttca
    11941 cctgctctca ggtcatatac cgcttcactt accgttatgg gctcaggtga aacaatgaag
    12001 gctctcataa ctccctcatg ctcccctgag tatacaatat caggctcggt atattctaaa
    12061 accatattat acggatttga cgtaaattcg ccagtattcc ctgaatcatc agttgccttt
    12121 acatgcaagt aataggttcc gctcactccg gaaaagctta cggtattacc attgggattg
    12181 tagtcatcca taggtatctc cgtccaatct gtaacatcct ctatattgct ttcagtagta
    12241 aaggcgtact caattcttgc tatgttttta ttgtcgctga cttttatttt aggtgaaaca
    12301 ctcattgcat tgctatgatt gccgttggga gtgactacaa tcgttggcga ttcggtgtcc
    12361 ggaggctccg gttccggctc atcatcatcg tcatcatcgt caacaaccgg ctgaattacc
    12421 acaggtatag gttccacaca tgcctttacc gctcccttat tcccggcctc atcctccagt
    12481 ataaagtaat gaggttctcc cctggcaatg atttccttgt agtatccggg aacatatcca
    12541 attaccttta tatcctcgga agggcacaag ttgtcactga tgtcaacaag ttccgcagta
    12601 aagaaatagt tcgaaccata gtagctgctg tacttgaccc ttgccgtagg aggagttaca
    12661 tcccttataa ccgtagatgc agctgcatcc gactctttcc ctgcactatc cctgtatttt
    12721 gtatagaagg ttatgggccc ttccttgtca ggcaaaggaa tacaagggct gtaaccctgg
    12781 gtttcaaagg aagtagcccc tttttcaagg ggtatccagc cgctccagct tactccgtcc
    12841 atggaccagg agaattcctc tacagagttg aaaatatgga gctctgcaaa atcctcattg
    12901 gtgtatttgc ttcctctgta aaaattaatg tatggggctg aggctgaaag ctttgattca
    12961 gcattgccgt atggcccgaa aagcatattt ctgttgttct ggtgcgcgtc cgtcgccgtt
    13021 acattcacat aggtatccgg cttcagttca tccttgccta ttattatttc cccgttctcg
    13081 ggaagctgct gccggccgga attacctaca atgtactttg ctgtgatatc cttccgggga
    13141 gtataattat cggatacatg gatcgtgacc ttcatggatc cgtccgcctg catttccgca
    13201 tttgcattca cctggggagg cttgaagtcg aaaagcagcg gctgcgggac cgttttgctt
    13261 cctataggct tcctttcctc gtctgccgca atgatgtgca aataataata gccgtccctc
    13321 tcccggggat ctccttcaaa atcttccgag gtattgatcc tgtggctctc cttgtacctc
    13381 catatccctg agttctctga aaactcaggc attacaggtt cccgggtcat gcagtaccag
    13441 agccccttgc agcgccaatt cacgccgtct tcccttaccc agtctgcaga gcataaatag
    13501 acccttcctt cagcggcgcc ccccaggtta gtctcaagac tgacgctcat ttctttttga
    13561 gtctcaatgg aggatatgtc aatatccgtt ctggtcacat ttatgtccgt cgcccagccg
    13621 tatcctatat atccatgggt cgagccgtag aacatattgc cctttttgtc ctttgcatgt
    13681 acaaataacc tgtagttact tgttctgccc ggatcgatcc cgaacattcc ggccaactcg
    13741 tgggaatggt accatctgcc cggagccagg gatttcgcag ctctcataat acctccgaag
    13801 tccgcgttgg tatacccaat gccgaacttc aaggtatcat aatccactcc cgaaacctca
    13861 tccgtaacat ttatctttac gtaaaagcct cccttttcct tttccgcttc cttggtgtgg
    13921 acggtaatta ccggaggctc ggtatctccc acatatcttt tactatccga aagcttcact
    13981 cctcctatgg gaagcagcgg cgccttgttc cctgctgcat ccatcacata cagctcccca
    14041 taggaaactc gttctgtcga cccgctttcc ttccacttgc tgtatatact ccctgttatc
    14101 tcaaggggat cgaagctgtt ctccatgttg tcgaaacgaa attccagcat tgttgtgccg
    14161 ttcccactcg catagtatgc atcaggggta ccggagcggt acggatccaa tgcccctgtc
    14221 cagtcccggt tcaatgcgtt cagatacagc ttcgcatcgt agcttgaaaa tctgcctgcc
    14281 ctctgatagt tctcaaactc cagcttgttt atgttatgtt tgataaaggc tccatcggat
    14341 atcgttatat atacaggctc cgaaaatttg aaatacaaag gcaatgtttg agaacggtct
    14401 aacgttccct cgggcagtcc gccgtttatc tccagcagta ccggagctgt cgtttccact
    14461 ctgaaatccc gggtatagat cctgtagaca ttgctgactc cgcttgcgga gcctgaggca
    14521 aaggatatga tcctgtattc attgggcatc gggttgaaag cattgtctgt gatgactccc
    14581 ccctccagca ccatttcata tatgtctgag gctctgaact catctcccgg ccgcacggta
    14641 tatttgaact ttatctgctt gctgtaatca tttccgtagg tctccttatt atacctggat
    14701 ttgtcaaccg ggtaggcata tccctccata tcccttccgt cggcgttcct gtacttgaac
    14761 ttcatcttca gctcgccgaa ttgctgcaat atcccccttc cgttgttgtc ccgcacatgc
    14821 tccaacagca gcttttcatc agggtaattt ggggacagct gggcatttag aaatgaattc
    14881 ctctcggtgg cgttcaagcc cctcacataa gccagataac ccggatcgtt tatgaatatg
    14941 ggttcgttaa actcaaggtc ccaatataca tcgtccccta tgccgtagta atccttcatc
    15001 cttctttcct cgggagtccc cagttcccgc ctttctccga attccacata tttgggtata
    15061 ggggccacat catcagttat atatacctct atattcctga aatatatttc acatttgtta
    15121 tcatcaagta tactgatgcc cttctccccg gcaagggtca gctgcatacc ggttgcaccc
    15181 tccggcacat agcccgaaaa gcttacatca ttccagccgc tgaaatcatg ctgcatccgt
    15241 tccaccgtta ttgaaccgct tctcccgtca cccaacggag cccagaagtc caggaatata
    15301 ctagccacat ctccatcgaa agcctgtccg taagcctgca gccttacctt gtatttcagc
    15361 ccggcttgat tgtcggcgcc cttaaggggc atggtccaaa agtatttaaa actattattt
    15421 atatttttag gcttgatttc atcccctgtc tcgggatggg actcaatata aaagctgttt
    15481 gtctcgtttg tgctccattg tcccttgtca ggataacagt tttttatccc cttggatgca
    15541 aaatgtgctc cgttgtcaaa gcgcacatcg gtacccgacc ccgcaaccgg gacaatctgc
    15601 gatgtaagca ataccaatgc cataattacc gatatcatac ctttgactgt caagtttcgc
    15661 ataaaaccac cctcttacat ttataatttc atactcagcc aagcagaata acatccataa
    15721 gctctcctac ggtaagccgt acccttccgt tcccccatag gacataatcg ccgtcagtta
    15781 c
//
LOCUS       DAOQUR010000052        15671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4273_length_15671_cov_12.277024, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000052 DAOQUR010000000
VERSION     DAOQUR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15671
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4273_length_15671_cov_12.277024"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1081)
                     /locus_tag="PKI50_09690"
     CDS             complement(323..1081)
                     /locus_tag="PKI50_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT04012.1"
                     /translation="MVSIGIDIGSVCAKAVAYDERIIGRLIMPTGWNPRQTAKELYER
                     LVSENEIKKGEIASVVATGYGRVSVPFASRKVTEITCHAKGAACLDPDARTVIDVGGQ
                     DSKAISIDEKGNVLEFMMNDKCAAGTGRFLQVMANVLETEVDQLDALASNSTPRDINS
                     MCAVFAESEVISLLSQGTSKEAVASGILHSISNKIIHLASRITIREKVLFTGGVASNS
                     MIREIISKKLKCRVLTHKDSQYAGAIGAAIIGYY"
     gene            complement(1109..1363)
                     /locus_tag="PKI50_09695"
     CDS             complement(1109..1363)
                     /locus_tag="PKI50_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNT04013.1"
                     /translation="MEEECFYVLFPNVTEGLKLEKLLKEKAVKYTIVPTPRELSKSCG
                     ISIMYEFRDDSLIKRIAEEYGIRTEGFKSLTRKKAEVKFI"
     gene            complement(1363..2634)
                     /locus_tag="PKI50_09700"
     CDS             complement(1363..2634)
                     /locus_tag="PKI50_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HNT04014.1"
                     /translation="MADYRELWSNLGMDLEKHDLLCEALPQLFGGVYLIQENRPEGMN
                     YYNFVVSEVHGLRIKELDDYKKAGGKVVGTFCVFVPDELILAAGAIGVGLCGGSQFWI
                     EDGEKVLPRNLCPLVKAIVGAKISGTCPYFQSCDLLTGETTCDGKKKAYEVLGEYAPV
                     YVMDLPQMKRDKDYKKWEEEIYEYKAVLEELTGNKITEEKLGSAIRLCNRKRAALQRL
                     YNARKALNVPISGKDALLVSQIAFYDNSERFIEMTNKLCDELEDRIKKGIGVASEKSP
                     RIMITGTPMAIPNWKMHHLIEESGAVVVCEETCTGTRYFENTVDETQTTMEGMIKALA
                     DRYMNINCACFTPNKGREEDVVRLAKEYGADGVIYCNLQFCHTYNIEYRQVEKALKEA
                     GIPVIFIETDYSNDDTGQLQTRIEAFLEMLK"
     gene            2867..3736
                     /locus_tag="PKI50_09705"
     CDS             2867..3736
                     /locus_tag="PKI50_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT04015.1"
                     /translation="MNYIIAKLKNDRTRSIIFMIIAALLWSTGGLLLKGLDLNPMAVA
                     GSRSLVAALLMLAIIKKPSLKFNRYKLGFAVAFAATMILYIAGNKLTTAANAIMLQYT
                     APIYVALLGAWFLNERTSRLDWIAIFFTVGGMFLFFLDDLSSGNIIGNILSILSGVSF
                     AFVVMMLRKQKDQSPIELVFWGNLLTAAIGVPFLPGSAPDQSDLVMLVIIGVFQIGLP
                     YVFFAYGTKHVSALEAVLIPVLEPILNPVWVFLLMGEKPGRFALLGGFIVLLAIVGRG
                     VILTLKTKKNPPA"
     gene            complement(3760..4068)
                     /locus_tag="PKI50_09710"
     CDS             complement(3760..4068)
                     /locus_tag="PKI50_09710"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNT04016.1"
                     /translation="MTTENNLFIILDTTLTKELIEEGYAREFISKVQQMRKNNAFEMM
                     DRINIYYEGDDEIAEAVKAYEEYIMTETLAEKIERVTDANFEVQDLNGHQTGMKVEKI
                     "
     gene            complement(4097..4798)
                     /locus_tag="PKI50_09715"
     CDS             complement(4097..4798)
                     /locus_tag="PKI50_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNT04017.1"
                     /translation="MKQLPKAVTFDNFGTLIDWEGEIQKYMAKFLERNGIKEVDPKTV
                     QHRWEEIQFDYIQGDYKPYRKVLHDTLAMTFKEFGYAFTEEDCVEFSDSMGTWQPFPD
                     SRDALLELKKYCKIVLITNTDNDIIKESVKQLGVEFDDVITAEMAGAYKPSHKGFDLA
                     RQRLGLKKEEILHAGFGFKYDVVPATELGYETCWINRQGEIRPIDVKETYLVGDMRTF
                     VYMIKGMAQTFAEQK"
     gene            4994..5197
                     /locus_tag="PKI50_09720"
     CDS             4994..5197
                     /locus_tag="PKI50_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNT04018.1"
                     /translation="MDSTKCKVFITSVELGSFSKAAAALGYTISGVSHLVNAFEKEMG
                     FPLLTRDKKGVKPTNNALKIIPV"
     gene            complement(5460..6443)
                     /locus_tag="PKI50_09725"
     CDS             complement(5460..6443)
                     /locus_tag="PKI50_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNT04019.1"
                     /translation="MLILRKSDIEKAISMDEAISAMETAFIKFSEGNTKIPLRTVINV
                     EDKGDAIFMPGYVEGLGVAVKLVSVFPGNLEIGKPTINSVILLEDQNTGEVLALMDGG
                     CITALRTGAASGLATKYLARTDSKSVAVIGAGVQAKTQLWAVCSVRDIQEARIFDLND
                     DRKKRYIEEMSEKFPYIRFAGVSNTDEAVKGADIIITATTSKKPVFSAENVSKGAHIN
                     AMGAYTPEMQELPEKVLKMADKIVVDSREAVLKEAGDLIIPIRKGIFSEKQIYGELGE
                     VASGIIPGRQTDKEITLFKTVGIAAEDVACAAKIYENALLMGLGEEIFLDN"
     gene            complement(6512..7036)
                     /locus_tag="PKI50_09730"
     CDS             complement(6512..7036)
                     /locus_tag="PKI50_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT04020.1"
                     /translation="MKNEIIFSGVGGQGIMLIGKMLCEAAVDKGYQVTFAPTYGQEKR
                     GGRTSCQIVISDSVDSPVISEADTVLIMDEKSLDDFKDRVKKGAYLIINSSMVEKELT
                     RDDINVIKVPVNDIASEMGNPKSANIVALGVALKYIDSVSLENIKEYLGKIFKGSAIE
                     KNKKALQTGYEYSV"
     gene            complement(7033..7782)
                     /locus_tag="PKI50_09735"
     CDS             complement(7033..7782)
                     /locus_tag="PKI50_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT04021.1"
                     /translation="MKVVYKRPELLTDAHFSYCGGCGHGIINKLLAEIIEEEKLAEKA
                     ILVWPIGCSVYADQNFKVDSICALHGRAPSVGTGLKRALPENTVIVYQGDGDMVSEGM
                     AEIMHAAIRGEKFSVIFVNNAIFGMTGGQMAPTTLLEQKTTTSPYGRRAETSGYPVNM
                     AELISNVRGCYFSERVYVNNPKNIMNAKKAIKKCIQYQMEGKGLSFVEVVSQCPTGWG
                     VNPLDSLKMVETEMSKVYPLGVFKDMGVNDN"
     gene            complement(7795..8862)
                     /gene="vorB"
                     /locus_tag="PKI50_09740"
     CDS             complement(7795..8862)
                     /gene="vorB"
                     /locus_tag="PKI50_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNT04022.1"
                     /translation="MGEKVFMQGNMALAEAAIRAGCKYYFGYPITPASEIMEYYAAKM
                     PEENGVFVQAETEVSASNMIAGVAAAGKRAMMATSGPGISLGAEAISYMCAAQLPAVL
                     VNVMRPGPGDGDIVACQGDYFQAVKGAGHGDYNTIVLAPYSAQEMVDFAFKAFELAEK
                     YRNPVMILSDGGLAKMMESVELPDRIAPDTQFPWALTGAKNRDANVITTCAKSPKQWE
                     EYNLVLQEKFIKIKDAEQKWESYKTEDADVVVVAFGISARIAFSAVKMARDNNMKVGM
                     IRPITLWPFPEKAFKGLDDKYFFVTELNSGQMVEDVKLSVKDKEKVSFYGRLGGMTPT
                     PKELYEKLIELYKEHKLNANI"
     gene            complement(8865..9092)
                     /locus_tag="PKI50_09745"
     CDS             complement(8865..9092)
                     /locus_tag="PKI50_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT04023.1"
                     /translation="MNLVKIDMEKCKECGYCINFCPKKVVLEQGDSINKKGYYPAAVI
                     NQKECTACGTCAKVCPEAAIEVIKDFEGQEV"
     gene            complement(9171..9593)
                     /locus_tag="PKI50_09750"
     CDS             complement(9171..9593)
                     /locus_tag="PKI50_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HNT04024.1"
                     /translation="MVGKKVLLRARMSSGDAHYAGELVNGSKILDYFGDLGTELCIRY
                     DGDESLFLSYNSVEFLAPVYAGDFMEYYGWIEKAGNSSRVCKFEAYKVIELARDPNLA
                     LSAANVLAEPLLVGKAEGILVVKKGLQRGAQDPDFKHE"
     gene            complement(9624..10394)
                     /locus_tag="PKI50_09755"
     CDS             complement(9624..10394)
                     /locus_tag="PKI50_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT04025.1"
                     /translation="MEKKPYYLIQSVVRTLQIIEYLAETGESSVKEVAEFIGESKTTA
                     HRFLASIKQEGFLEQDSESKKYKLSLKLFEIGNKIVDTLDLRVIARPILQELSLLSLQ
                     TINLGILNKSDIVYIDKAISGNNLRLDSPIGGRDPAHCTAMGKSILAFLSKSIQESYV
                     SEYGLSMRTANTITNPTDLYIELDLVKQKGYAMDREELALGIECVAAPVFDSFMHPIA
                     AISISMPNNPENMGRMEEYQEQILIASKKISSKLGAKI"
     gene            complement(10404..11369)
                     /locus_tag="PKI50_09760"
     CDS             complement(10404..11369)
                     /locus_tag="PKI50_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HNT04026.1"
                     /translation="MSLNVVITDCSWGNIEVEKEYLPENAVVEGYQLITEDEVIEACS
                     DADAILSEYAPITAKALKALKKCKIISNSAIGFDNIDVKAAEELGIAVANVPGYCAHE
                     VADHAMSLILAASRNIVVYDKAVRKGEWNYEIGLEMDRLKGQVLGLAGFGKIAQFVAK
                     RAQGFGFKVIANDPFIKQEYADSLNVKLVEIDKLLAESDIISCHIPAIKDTIGYFNKE
                     KFDKMAKCPIFINTSRGRLVNEEDLADALENKKIRAAALDVLIEEPPTMNNRLFTFDN
                     VIITPHAGFFSKTALREVRSRSAMNVTNFFQKEFNKINFVNKIRG"
     gene            complement(11557..14655)
                     /gene="ileS"
                     /locus_tag="PKI50_09765"
     CDS             complement(11557..14655)
                     /gene="ileS"
                     /locus_tag="PKI50_09765"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNT04027.1"
                     /translation="MDKFKFLSEGRIEENEKRISEYWNEIDILKKCVETREGKEPFIF
                     YEGPPTANGKPGLHHVISRTLKDTVCRYKTMKGYQVKRKGGWDTHGLPVEIEIEKELK
                     LTGKQDIEKYGIKEFNQKCRESVFKYVGMWRDMTEKMAFLIDMDNPYVTYENEYIESA
                     WWILKKFKDEGYLYEGHKISPYCSRCGTGLATHEVAQGYKEIKTTTVIVKFKKKDANE
                     YFLAWTTTPWTLPSNVALTVNPKETYLRVRQNDEVYYVEKTLAAKVLGEDFEVLEELR
                     GTEMEYMEYEQLIPFVKADKKAFYVTVADYVTTEDGTGIVHTAPAFGEDDYNTGRKYD
                     LPVLQPVSMEGKFIGTPWEGKFVMDADLDIIKWLAAEGKLFKKEKVDHNYPHCWRCGT
                     PLLYYAKPSWYIEMTKLRDNLVAYNNAVNWFPPYVGEKRFGNWLENVNDWALSRDRYW
                     GTPLNIWRCDCGHTDSIGSIKELVDRAIEDIDENIDLHRPYVDEVHIKCEKCGGTMTR
                     VKEVIDCWFDSGAMPFAQHHYPFENKENFHELFPANFISEGIDQTRGWFYSLIAISTF
                     VMKTAPYRNVLVGGHLVDKDGKKMSKSLGNAVDVFDMFEKYGADALRWYMLYVSPVWT
                     TTKFDEDGVKDVLSKYFGTLKNVYTFFALYANTDSIDPKDFFVEYKDRPELDRWILSK
                     YNNLAAEVNAALDVYDLTRAARKLHTFVNEDLSNWYIRRSRRRFWDAELTEDKKAVYN
                     TTYEILVGVSKLMAPLAPYLPEEIYRNLTGELSVHVAYFPEVNEDLIDNKLEERMDLV
                     RSLVGLGRASREQVRIKVRQPLQKVFIDAKYEELISYLIPLIQEELNVKEVIFEKNLN
                     KFIDFTLKPDYKFAGPVLGAKIKAFAKMLEEMDAAVAAPKFEAGETVKIELEGEPLEV
                     NKDYVITRIAAKEGFTMTTENNLFIILDTTLTKELIEEGYAREFISKVQQMRKNNAFE
                     MMDRINIYYEGDDEIAEAVKAYEEYIMTETLAEKIERVTDANFEVQDLNGHQTGMKVE
                     RI"
     misc_binding    complement(14719..14941)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15068..>15671)
                     /locus_tag="PKI50_09770"
     CDS             complement(15068..>15671)
                     /locus_tag="PKI50_09770"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HNT04028.1"
                     /translation="LDGYVGLLVSALIIYSGISLTKDTLNPLLGEAPEPKFIRSITEK
                     VLSYDGIIGIHDMIVHNYGPGRCVVSLHAEIPASMDIMKAHDVIDLAEQEISEEMDIH
                     MVIHMDPINTDDEVVQKTQEQITGLINEMHEELTIHDFRIVGGEEHKNLIFDLVVPFE
                     YDEKMAGDLSKKVAWEIKSKYPECNAIIYIDRAYSVLDKL"
BASE COUNT         3955 a         3814 c         2705 g         5197 t
ORIGIN      
        1 cttttccggt aatatgtata tattatacat catataggcc taagatacga gactccggat
       61 atgataggag acagggcacc ccccattgta taggaaagtg tgctttccta tacaataaaa
      121 aagaacgcgg accactccgc gttcgaggct gatgaaaaac ctcctatata agaacagtaa
      181 atgagacacg gggacgtttc tcctgtctca ctgaagtgag acacgcgaaa cgtccccgtg
      241 tctcatttgc tgttcctatt atgagctttg tctgcaaact gaaacgttta atactccacg
      301 ttctcaaaac tatatccaat catcagtaat atccaattat tgcagcgcct attgcccccg
      361 catactgcga gtccttatgg gtcaaaacac ggcacttcag cttttttgat ataatctccc
      421 ttatcatgct gttggaggca actccccccg taaagagcac cttttctctt atggttatcc
      481 tgcttgccag atgtattatc ttgttggata ttgagtgcag tatgccggaa gcaaccgctt
      541 cctttgatgt gccttgtgaa agaaggctga ttacctcgct ttccgcaaaa acagcgcaca
      601 tgctgtttat atccctgggt gtcgaattgg aagccagagc atccaattgg tcaacctccg
      661 tttcaaggac attggccatt acctgcagga accttcccgt accggctgca catttgtcgt
      721 tcatcataaa ttccaatacg tttccctttt catctattga tatggccttg ctgtcttgcc
      781 cgcctacatc aatgaccgtg cgggcatcag ggtccagaca tgccgccccc tttgcatggc
      841 aggttatttc ggtaaccttt cttgatgcaa aggggacaga tactctgccg taaccggtag
      901 caacaacgga ggctatttcc ccttttttta tttcattctc cgagaccagc ctctcataca
      961 gctcttttgc cgtttgtcta ggattccagc ctgtgggcat aataagcctg cctattattc
     1021 tttcatcata agcaaccgcc tttgcacata ccgagcctat atctattcct atactaacca
     1081 tgacagctcc tcttcccctg cccctttttc atatgaactt aacttcagct ttctttctgg
     1141 tcaggctttt gaagccttct gttctgattc cgtactcttc tgcaatacgt tttattaagc
     1201 tgtcatcccg gaattcatac attatgctta ttccgcagga cttgctcaac tctctcggag
     1261 tgggcactat agtgtatttg acagctttct cttttaaaag cttctccagc ttcaagccct
     1321 cggtgacgtt gggaaataac acgtagaagc attcttcctc cattatttta acatctccag
     1381 gaatgcctca atccttgtct gaagctggcc tgtatcatca ttgctgtagt ctgtctctat
     1441 gaatattacc ggtatccccg cttctttaag agccttttcc acctgcctgt attcaatgtt
     1501 gtaagtatgg cagaactgca gattgcaata tataacgccg tctgcgccat attcctttgc
     1561 cagccttacg acatcctctt ccctaccctt gttgggagtg aagcatgcac agtttatgtt
     1621 catatacctg tctgccaggg cctttatcat gccttccatt gttgtctggg tttcatcaac
     1681 cgtattttcg aagtaccttg tccctgtaca ggtttcttca cagacaacca ctgcaccgct
     1741 ctcttctatc aggtgatgca ttttccagtt tggtattgcc atgggagtac cggttatcat
     1801 tattctcggg ctcttttccg aagccactcc tattcccttt ttgatcctgt cttccagctc
     1861 atcgcacagc ttattggtca tttcaataaa tctttcggaa ttatcataga acgctatctg
     1921 tgaaacaaga agcgcatcct ttccgcttat aggaacatta agagcttttc tcgcattata
     1981 cagtctctga agtgcagccc tctttctgtt gcacagcctg atagccgacc caagcttttc
     2041 ttccgtaatc ttatttcccg tcagttcttc aagcactgct ttatattcat atatttcctc
     2101 ttcccatttc ttatagtcct tatccctctt catctgcgga agatccataa cataaaccgg
     2161 tgcatattcc ccaagaactt catatgcctt tttctttccg tcgcaggtgg tttcgccggt
     2221 cagtaaatca caggactgga agtacggaca ggttccgctg atttttgcgc ctacaatagc
     2281 ctttaccagg ggacaaagat ttcgaggcag tactttttcg ccgtcctcaa tccagaattg
     2341 cgagcctccg cagagcccga cccctatggc ccctgccgcg agtatcagct cgtcgggcac
     2401 aaatacacag aaggttccta ctacctttcc tccggccttt ttatagtcat ccagttcttt
     2461 tatcctcaac ccgtgcacct cagaaaccac aaagttataa taattcatcc cttcaggcct
     2521 gttttcctga atcaggtata ctcccccgaa taattggggc agtgcctcac aaagcaaatc
     2581 gtgtttttca agatccattc ccaggttact ccaaagctct ctgtaatctg ccattttttt
     2641 acccccttaa cataattgag gcactatggc gcagttaatt gcctataatg cccgtttaac
     2701 atatttatat tttatatcat cggattcgaa tataataatt gtttttcgct ataatgcagc
     2761 atgatagtat ttaaaatatg aatatatccc atgcctgaga cgctccattg aattgtcccc
     2821 tttgttctgt tagaatctat atatactcag gagggactaa aacattatga actatataat
     2881 agcaaaatta aaaaatgata gaacaagatc tataattttt atgataattg cggcactgct
     2941 gtggagtacg ggaggcctgc tgcttaaggg cctggatttg aatcctatgg cggtagccgg
     3001 ctccagaagc cttgtagcag cgctgcttat gcttgcaata attaagaagc ccagtctgaa
     3061 attcaacagg tataaactgg gttttgccgt tgccttcgca gcaaccatga tattgtatat
     3121 agcgggaaat aagcttacaa ctgccgcaaa tgcgattatg cttcagtata cggcacctat
     3181 ttatgtagcc cttctggggg catggttcct caatgagcgt acctcaaggc tggattggat
     3241 tgctattttt tttacggttg gcggcatgtt cctattcttt cttgatgacc tttcatcagg
     3301 aaatataatc ggaaacatat tgtcaatatt aagcggggta tcctttgctt tcgttgtgat
     3361 gatgctcagg aaacaaaagg atcaatcacc tatagagctg gtattctggg gcaaccttct
     3421 tactgccgca ataggggttc cttttcttcc gggctctgca ccggaccagt cagacctggt
     3481 aatgctggtt attataggtg tgttccagat tgggctgcct tacgtgtttt ttgcatacgg
     3541 aaccaaacat gtttcagccc tggaagcagt attgatacct gttctggaac ctattctgaa
     3601 tcctgtgtgg gtgttccttc tgatgggtga aaaaccggga cgctttgcgc tgctgggcgg
     3661 cttcatagta ctgcttgcca tagtcggcag aggtgtaatc ttaacgttaa agacaaaaaa
     3721 aaatccaccg gcttaactta gcggtggatt tttgctgctc tatatttttt caaccttcat
     3781 tcccgtctga tgtccgttaa gatcctggac ttcaaagttc gcatccgtca ccctttctat
     3841 cttttcagcc agggtttcgg tcatgatgta ttcctcatat gccttgactg cttctgctat
     3901 ttcatcgtcc ccttcatagt atatgtttat tctgtccatc atttcaaaag cattgttctt
     3961 tctcatttgc tgaaccttgg agatgaactc tcttgcgtaa ccctcttcaa taagctcttt
     4021 tgtcagggta gtatccagta ttatgaagag attgttttct gttgtcatta cttatttctt
     4081 tatatttatt attctattat ttctgttcgg caaaagtctg cgccatacct ttaatcatat
     4141 agacgaatgt cctcatatca ccaacaagat aggtctcttt aacatctata ggacgaattt
     4201 ctccttgacg gttaatccaa caagtttcat agcccaattc agtagcaggg acaacatcat
     4261 atttgaatcc aaaccccgca tggagaatct cttctttttt tagccctaac ctttgtctgg
     4321 ctaaatcaaa ccctttgtga gaaggcttat atgccccagc catttccgcc gtaatgacat
     4381 catcaaactc tacgccaagc tgtttcacag attctttgat aatatcatta tcggtattcg
     4441 ttataagtac aattttacag tattttttca actccaacaa agcatcgcgg gaatccggaa
     4501 acggctgcca cgtgcccata gaatcagaga actccacaca atcttcttct gtaaacgcat
     4561 agccaaattc tttgaatgtc atcgccaggg tgtcgtgaag aactttacga tacggtttat
     4621 agtctccctg tatgtagtca aactgaatct cctcccaacg atgctgaact gttttaggat
     4681 caacttcttt aatcccgttt ctttccagga actttgccat atacttctga atttcgcctt
     4741 cccagtcaat caaagttcca aaattatcga atgtcacagc tttaggtaat tgtttcattt
     4801 ttacacctct ttttctttca ctatattttc ctttttattc tctgccatat gcataaaatc
     4861 actttggaaa tatgattttg tatcgggcta ttgttgtttt gctgattgca ttgtataatt
     4921 tttttaacat taagtaaata tacagttatt gaaatttgat ttaagtgtta cttaaatata
     4981 agcgaggagt tatatggata gtacaaaatg caaagtattt attacgtcag tagaacttgg
     5041 cagtttctct aaggcggcgg cagcattggg atatacgata tcgggagtaa gccatctggt
     5101 aaatgcattt gaaaaggaaa tgggttttcc tttactcacg cgggataaga aaggtgtaaa
     5161 acctacaaat aatgcgttga aaatcattcc cgtttagaga ggaaacataa atatcttttc
     5221 taaaggtgtg ctgccaaaga tgcaaaatgt taatagcaaa aatattgaag cataagtttt
     5281 ttgaacaact gctgtgaccc accaggtaat tgttaataat aacgatgcac atactatgct
     5341 ttggttggta ttaaatccaa aaggttgata aagaagtata ataatcggtg caattaaact
     5401 tagaatcagc ttttttttca ttttatcacc tgccaagacc acaaaagaaa ttgatcttat
     5461 taattatcta aaaatatttc ctctcccaaa cccatcaaca aagcattttc ataaatttta
     5521 gccgcacatg ctacatcttc cgccgcaatt cctaccgttt taaatagtgt aatctcctta
     5581 tcagtttgtc ttccgggaat tataccggag gcaacctcac ctaattcgcc ataaatctgt
     5641 ttttcagaaa agatcccttt tcttatggga attattagat cacctgcttc cttcaaaacc
     5701 gcttcccttg aatcaaccac tatcttatcc gccattttta atactttttc cggcagctcc
     5761 tgcatttccg gagtgtaagc ccccatagca tttatatgag ctcctttgct tacattttca
     5821 gctgaaaaca cgggcttttt cgaagtagta gccgtaataa ttatatccgc tcctttaacg
     5881 gcttcatcag tatttgatac tcctgcaaat cttatgtaag ggaacttctc gctcatttct
     5941 tctatatacc ttttcttcct gtcatcgttc aaatcgaata tccttgcttc ctgaatatcc
     6001 cttacactgc aaactgccca caattgtgtc ttggcctgaa caccggcacc gataacggct
     6061 acgctttttg aatctgtcct tgccaaatat tttgtcgcaa ggcctgaagc ggctccggtc
     6121 ctgagagcag ttatacaacc tccatccatc aaagccagaa cttcacctgt attctgatct
     6181 tcaagcaata taacagagtt aattgtgggt ttcccaatct ccaaattacc tggaaaaaca
     6241 gaaacaagct taacggccac accgagtcct tccacatatc ccggcataaa tatcgcatcg
     6301 cctttatcct caacattgat tactgtcctc aaaggtattt tggtatttcc ctcggagaat
     6361 ttgataaaag ctgtttccat cgcactaatg gcttcatcca tagaaattgc tttctcaata
     6421 tcgctttttc ttaatattaa caatgaaacc cctccattct tactaattat ttttggcttg
     6481 tacaaaccag ctgatatgag tcgataacga ttcatacact atattcataa ccggtttgca
     6541 aagctttttt attcttttcg attgcactgc ctttaaagat cttccccaaa tattctttta
     6601 tgttttccag cgataccgaa tcaatatatt ttaatgctac cccgagagct acaatattag
     6661 cggactttgg atttcccatt tcgctggcaa tatcatttac aggaactttt ataacattga
     6721 tatcatctct tgtaagttct ttttctacca ttgagctatt tattatcaga taagcgccct
     6781 tcttaactct gtctttaaaa tcgtccaggg atttctcatc cattatcaat acggtatccg
     6841 cctcggaaat aacaggtgaa tctacgctat cggatattac aatctgacag ctggtacgtc
     6901 ctcccctttt ttcctggccg tatgtaggag cgaaagtgac ttgatatcct ttatctactg
     6961 cagcttcaca tagcattttc cctatcagca ttattccctg accgcctacg cctgaaaaga
     7021 ttatctcatt tttcaattat cattcacccc catatccttg aatacaccaa ggggatatac
     7081 cttggacatt tctgtttcca ccattttcag actatctaac ggattcactc cccagccggt
     7141 cggacattgt gacacaactt caacaaagct tagccctttt ccctccatct ggtattgaat
     7201 acactttttt atggcttttt ttgcattcat aatattctta ggattgttaa cgtatactct
     7261 ctcactaaaa tagcatcccc ggacattgga tatcagttct gccatattca caggatatcc
     7321 gctggtttca gcccttctgc catatggtga ggttgttgtc ttttgctcaa gcagtgttgt
     7381 cggggccatt tgtccccctg tcattccaaa tatcgcattg tttacaaaaa taacactgaa
     7441 cttttctcct ctgattgcag catgcattat ttccgccata ccctccgata ccatatctcc
     7501 gtcaccttga tatacaatta ctgtgttctc cggaagagct cttttcaaac cggtacctac
     7561 cgatggggca cgtccatgca atgcacatat tgagtcaacc ttgaaatttt ggtctgcata
     7621 aacggagcag cctataggcc ataccaggat ggctttttca gctagttttt cttcttctat
     7681 tatttctgcc aataatttgt taattattcc gtgtccgcaa ccgccgcagt atgaaaaatg
     7741 tgcatcagtc agaagctccg gtctcttata tactaccttc attacatcca ccccttaaat
     7801 gttagcgttg agcttatgct ctttatataa ttcaatgagt ttttcgtata gttcctttgg
     7861 agttggggtc atcccgccaa gcctcccata gaaactgact ttttctttat ctttgactga
     7921 taatttcaca tcttccacca tctgaccgga atttaattct gtaacaaaga aatatttgtc
     7981 atcaaggcct ttaaacgctt tttccgggaa gggccaaagt gttatcggcc taatcattcc
     8041 aaccttcata ttgttatccc ttgccatctt aacagctgaa aaagcaattc ttgctgaaat
     8101 gccgaaagca actactacta catctgcatc ttcggtttta taactttccc atttttgctc
     8161 cgcatcttta attttaataa acttttcctg cagcaccaga ttatactctt cccattgctt
     8221 tggtgacttg gcacaagttg tgatgacatt tgcatctctg ttttttgcac cggtcaatgc
     8281 ccatgggaac tgtgtatcgg gtgctatcct atccggaagc tcaactgatt ccatcatttt
     8341 ggcaagtccg ccatcactta ggatcattac gggatttctg tacttttccg ccagttcaaa
     8401 tgctttaaaa gcaaagtcaa ccatttcctg tgcagagtag ggagccaata ctatagtatt
     8461 ataatcgccg tgtcctgcac ctttaactgc ctggaaatag tctccctggc aggctactat
     8521 gtcaccatcc ccggggcccg gtctcattac attcactaaa actgccggta attgcgccgc
     8581 acacatatat gatattgctt ccgcacctaa gcttattccc ggtcctgagg ttgccatcat
     8641 agcccttttc cctgctgcag caactccggc tatcatattt gacgctgata cctctgtctc
     8701 agcttgtaca aagacaccat tttcttcggg catttttgct gcataatatt ccattatctc
     8761 gctggccgga gtaatcggat acccgaaata gtatttgcag cccgctctta ttgcagcttc
     8821 cgctaatgcc atattccctt gcataaatac tttttctccc atgcctaaac ctcctgccct
     8881 tcaaaatctt tgataacttc aatagcagct tccgggcaaa cctttgcaca tgtcccgcat
     8941 gcggtacatt ccttttggtt tatgacagct gcaggataat aacccttttt attaatacta
     9001 tcgccttgtt ccaacactac tttcttagga cagaaattta tgcaatatcc gcattctttg
     9061 catttttcca tgtctatttt tactaagttc aatcagaatt cctcctcaac tatctggcag
     9121 caaacctcca actttaatag ctggaggtca gacacctggt actgattacc ttattcgtgt
     9181 ttaaaatccg gatcctgcgc tcctctttgc aaacctttct taacaacaag gattccttca
     9241 gcttttccaa ccagcagcgg ttctgccaga acatttgcag ccgataaagc caggttggga
     9301 tctcttgcca gctctattac tttgtatgct tcgaatttgc agaccctgga tgagtttcct
     9361 gccttttcta tccatccata gtattccatg aagtcaccgg cataaacagg tgcaaggaat
     9421 tccaccgagt tgtaggacaa gaataaactt tcatctccat cgtacctgat acataattct
     9481 gttccaagat caccgaaata gtccaatatc tttgaaccgt taaccagttc acctgcgtaa
     9541 tgagcatccc ctgaactcat cctcgctctc aataaaacct tttttcctac catttataaa
     9601 tcctccttaa attttgttat tgtttatatt tttgcaccca atttacttga tattttttta
     9661 gatgcaatta gaatctgttc ctggtattct tccatccgtc ccatattttc cgggttatta
     9721 ggcatagaaa tactgatagc tgctatagga tgcataaaac tatcgaatac aggtgcagca
     9781 acacattcta ttcccaatgc aagctcttct ctatccattg cataaccctt ctgtttaacc
     9841 aaatccaatt ctatgtataa gtctgtggga tttgttatag tattggcagt cctcatactt
     9901 aagccatatt cgcttacata gctctcttgt atgctcttag ataaaaatgc aagtatcgat
     9961 ttgcccatag cggtacagtg agcaggatcc cttcccccaa tagggctgtc caatctgaga
    10021 ttattaccgc tgattgcctt gtcaatatag acaatatcac ttttgtttag tattcctaag
    10081 ttgatagttt gcaaagagag cagtgacagc tcctgaagta taggtcttgc tatcaccctc
    10141 aagtccaggg tgtcaacgat tttattgccg atttcaaaca gttttaagga caatttatat
    10201 tttttgcttt cactgtcctg ctccaaaaat ccctcctgct taatgctggc aagaaaccta
    10261 tgggctgtag tcttgctctc tccgataaat tcagctactt cttttacgct tgactcgccg
    10321 gtttctgcaa ggtattcaat tatctgcaat gttctcacaa ccgattgaat taagtaatac
    10381 ggcttctttt ccataatccc tcactaccct ctgattttat ttacaaagtt aatcttattg
    10441 aattccttct gaaagaaatt tgtcacattc attgcagatc tgcttctgac ctctcttaat
    10501 gcggtcttcg aaaaaaatcc tgcatggggt gtaattatca cattgtcaaa ggtaaataat
    10561 ctattattca tagtcggtgg ttcttctatc agaacatcta atgccgcagc ccttatcttt
    10621 ttgttttcta aagcatcggc cagatcttct tcattaacaa gtcttcctct tgaggtgttg
    10681 ataaatatcg gacattttgc cattttatca aacttttctt tgttaaagta acctattgta
    10741 tcttttatgg cgggtatatg gcaggaaata atatcagatt cagcaagcag tttatcaatt
    10801 tcaaccaatt tgacgttcaa tgaatcagca tattcctgtt ttataaacgg atcattagca
    10861 attaccttaa atccaaagcc ttgtgctctc tttgctacga actgtgctat cttgccaaaa
    10921 cccgccaaac caagcacctg tcctttaagc ctatccatct ccagccctat ttcatagttc
    10981 cattcacctt ttctcacagc tttgtcatat actactatgt tcctgctggc tgcaagaatg
    11041 agagacattg catgatccgc cacttcatgc gcacaatagc ccggcacatt ggctacagca
    11101 atcccaagtt cttcagcagc ttttacatca atgttgtcaa atccaattgc agaatttgaa
    11161 ataatcttgc attttttcaa tgcttttaat gctttggcag taataggagc atattcagat
    11221 agaattgcat ctgcatcaga gcatgcctca attacctcat cctctgttat taattgatat
    11281 ccttctacta ctgcattttc aggcaaatac tctttttcca cttcaatatt tccccagctg
    11341 cagtcagtaa ttactacatt gagactcatt taatcactcc cccttcgtca cacatagtgt
    11401 gacgtcgtaa ctcatatgtt tacgttagtt ttctacaagg atttttatat tccttcttta
    11461 atttaaaaaa aattttttaa ttttttagat ttttcttaat taaataataa ggccccccga
    11521 tttttccgga gaggccttta ttatttgctt tatttcctat atcctttcaa ccttcattcc
    11581 cgtctgatgt ccgttaagat cctggacttc aaagttcgca tccgtcaccc tttctatctt
    11641 ttcagccagg gtttcggtca tgatgtattc ctcatatgcc ttgactgctt ctgctatttc
    11701 atcgtcccct tcatagtata tgtttattct gtccatcatt tcaaaagcat tgttctttct
    11761 catttgctga accttggaga tgaactctct tgcgtaaccc tcttcaataa gctcttttgt
    11821 cagggtagta tccagtatta tgaagagatt gttttctgtt gtcattgtga agccttcctt
    11881 ggctgctatc ctggttataa cataatcctt gtttacctcc agaggctctc cttcaagctc
    11941 aatcttcaca gtctcccctg cttcaaactt aggtgctgct actgctgcgt ccatttcctc
    12001 aagcattttt gcgaaggctt ttatctttgc tcccagtacg gggcctgcaa atttgtaatc
    12061 cggtttcaag gtaaagtcaa taaatttgtt cagattcttt tcgaatataa cttcctttac
    12121 gttcaattct tcctggataa gcggaatcag gtaagatatc agctcttcat acttggcatc
    12181 tatgaatacc ttctgcagag gctgacggac ttttatcctc acctgttccc tggaagctct
    12241 cccaagtcct acaaggctcc ttacaagatc cattctctct tccagcttgt tatcgatcag
    12301 atcttcatta acctcgggga aatatgccac atgtaccgac agctctcccg ttaggttgcg
    12361 gtatatctct tcgggaaggt atggagcaag gggagccata agcttggata cacccacaag
    12421 tatttcatag gtggtattat aaaccgcttt tttatcttcc gtaagctccg catcccagaa
    12481 tcttcttctg gaacgcctga tataccagtt ggaaagatct tcgttcacaa aggtgtgcag
    12541 ttttcttgcc gctcttgtaa gatcataaac atcgagggca gcattaacct ctgctgcaag
    12601 gttgttgtat ttggaaagta tccacctgtc cagttccggc ctatccttgt attccacgaa
    12661 gaaatccttc ggatctatgc tgtcggtatt tgcatataat gcaaagaagg tgtatacatt
    12721 tttaagtgtt ccgaagtatt tgctcagtac atcctttacg ccgtcctcgt caaactttgt
    12781 ggtagtccag accggtgata cataaagcat ataccatctc aaagcatctg ccccgtattt
    12841 ttcgaacata tcgaatacat ctactgcatt gcccagggat ttggacatct ttttgccgtc
    12901 cttatccacc aggtgcccgc ccacaagtac attcctgtat ggagctgtct tcatgacaaa
    12961 ggttgatatt gctatcagtg aatagaacca gcctcttgtc tggtctattc cttcactgat
    13021 aaagtttgca gggaaaagct catggaagtt ttccttgttt tcaaaaggat aatgatgctg
    13081 tgcaaaaggc attgctccgc tgtcaaacca gcagtctatg acttccttta ctcttgtcat
    13141 ggtgccgccg catttctcac actttatgtg aacttcatcc acataaggcc tgtgcaggtc
    13201 gatgttttcg tcaatatctt caattgccct gtccaccaat tctttgatgg aacctattga
    13261 atccgtatgt ccgcaatcac atctccatat gttaagaggt gtaccccagt atctgtctct
    13321 ggaaagagcc cagtcattca cattctcaag ccaattgccg aagcgcttct ccccgacata
    13381 gggagggaac cagtttactg cattattata agccaccaga ttatccctga gctttgtcat
    13441 ttctatatac cagctgggtt ttgcgtagta aagcagaggt gtcccgcatc tccagcagtg
    13501 gggatagtta tgatccacct tttccttctt gaacagcttg ccctctgccg ccagccattt
    13561 gattatatca aggtctgcat ccattacaaa cttgccttcc caaggtgttc ctataaattt
    13621 gccctccatg ctgacgggct ggagcaccgg aaggtcgtat ttcctgcctg tattgtagtc
    13681 atcttcgccg aaggcaggtg cggtgtgaac tatgcccgta ccatcttcag ttgttacata
    13741 atcagccact gtcacataaa aagccttttt gtccgccttt acaaagggaa tcagctgctc
    13801 gtattccatg tattccatct ctgtgcccct gagttcttcc agcacttcaa aatcttctcc
    13861 caatactttt gcagccaggg ttttttctac gtaatatact tcatcgttct gccttaccct
    13921 gagatatgtc tcctttggat ttaccgtcaa ggctacattg gaggggaggg tccagggagt
    13981 cgtcgtccat gccagaaaat actcattggc atcttttttc ttaaacttta ctattacggt
    14041 agtggtcttt atctctttat aaccttgggc tacctcatgg gttgccagtc ccgtaccgca
    14101 tcttgaacaa taaggtgata tcttgtgacc ctcataaagg tatccttcat ccttgaattt
    14161 cttcaatatc caccaggccg actcaatata ttcattttca taggttacat acggattatc
    14221 catatcaatg aggaaggcca ttttttccgt catgtccctc cacattccca catatttgaa
    14281 aacagactcg cggcatttct gattgaattc cttgatgccg tatttttcga tatcctgctt
    14341 gccggtcaat ttgagttctt tttcaatttc gatttccacg ggaagcccat gggtatccca
    14401 gccgcccttc cttttgacct gatacccctt catggtcttg taccggcaga ccgtatcctt
    14461 aagagtcctg gaaattacat gatgcaatcc gggcttgcca ttggcagtag gaggcccttc
    14521 gtagaatata aaaggctctt tgccttctct ggtttctacg cactttttga gaatgtcaat
    14581 ctcattccag tattccgata ttcttttttc attttcttca atccggcctt ctgataaaaa
    14641 tttaaactta tccattttat acatcctttc cgaaagtgtg atctgtaaca gcatttaaaa
    14701 agtcccttcg gccaaagcca aagggacgat ataataccgc ggtaccaccc aggttatgca
    14761 tcaatcaaca aaatatgatg caccacttga cacatgtaac gcatgcctgc gggaaggctt
    14821 actgatttca gcccttcagc tccgaggtga tcttcataaa gtggcaaatg acaatctttc
    14881 accaaatgaa tgtctctctt tgaattgcgt cctttactac tgtcctcttc aatgctgtta
    14941 tgctataaat taatacccat tattatatat atattccgtt acaatgtcaa atgctatttt
    15001 gccctgtttg ctgatattta tcaatagctg tttattaata ttgcgccggc ctgaaacatc
    15061 cgcccgctta caatttatcc agaacggaat aagccctgtc aatataaata atggcattgc
    15121 attcaggata ttttgatttt atttcccagg cgactttctt tgataggtcc ccggccattt
    15181 tttcatcata ttcaaagggt accaccaggt caaatatgag gttcttatgc tcctcacctc
    15241 cgactatcct gaaatcatgt atcgtcagct cttcatgcat ttcgtttatc agcccggtaa
    15301 tctgctcctg ggttttttgc actacttcgt catcggtatt gataggatcc atatgaatca
    15361 ccatgtgtat atccatttcc tccgatattt cctgctccgc caggtcaatt acatcgtgtg
    15421 ccttcattat atccatgctt gcaggaatct cagcgtgcag tgaaactacg cacctgccgg
    15481 ggccgtagtt gtggactatc atatcgtgaa taccaatgat gccgtcatag cttaaaacct
    15541 tttccgttat tgaccttatg aacttcggct cgggagcttc tcccagcagc gggttcaaag
    15601 tgtcttttgt aagagatatt ccggaataaa ttattaatgc ggataccaga agccccacat
    15661 atccgtccaa g
//
LOCUS       DAOQUR010000053        15567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4303_length_15567_cov_9.747937, whole genome shotgun sequence.
ACCESSION   DAOQUR010000053 DAOQUR010000000
VERSION     DAOQUR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15567
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_15567_cov_9.747937"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..2068
                     /locus_tag="PKI50_09775"
     CDS             23..2068
                     /locus_tag="PKI50_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT04029.1"
                     /translation="MNKDKAANRLITEKSPYLLQHAYNPVDWYPWGQEAFDKAKAEDK
                     PVFLSLGYSTCHWCHVMERESFEDKEVAEILNKNYIAIKVDREERPDIDHIYMNVCQA
                     LTGSGGWPLTIIMTPEKAPFFAGTYFPKRARMGYPGVIELLEKINEAWNTEREKLLEA
                     SENILEHIRSDSAADSAVELDIETAGKAFKGLERSFDSEYGGFGRAPKFPTPHNLRFL
                     LRYWKVTNDPKALQMVEKTLISMYRGGIFDHVGYGFSRYSTDRIWLVPHFEKMLYDNA
                     GLAAAYLEAYQATGKEQYGEVARQIFEYVLRDMTSPEGAFYSAEDADSEGVEGKYYVW
                     SPKEITNILGEDAAVEFNGYYGLSDDGNFEGKNIPNLLHNKAAFENIRKNPLEGHRKK
                     VFEAREKRIHPYKDDKVLTAWNGLMIAALSIGARVLDNMEYAVAAERAVRFIGSKLIR
                     EDGRLLARYREGEAAYPGYLDDYAFLVWGLIELYEATFRAEYLKLAADLNRDMLKYFY
                     DEEKGGLFLYGSDSEQLIVRPKDVYDGAMPSGNSVAAMNFLRLGRLTGDTDLEDKAYK
                     QLEAFGSIIKGHPMGYADMLSALLFINSKASEIVIVGNREDENAKRMIKSINSHFSPF
                     TVVLFKDKENAEEGITSIAPYTEAQGMVDNKTTAYVCENFACRAPITDLQEFENVLA"
     gene            complement(2119..3012)
                     /locus_tag="PKI50_09780"
     CDS             complement(2119..3012)
                     /locus_tag="PKI50_09780"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNT04030.1"
                     /translation="MEIKRIKGVIPPMITPFKENGDVDYRMHAGNMEKWNNDNLAGCL
                     VLGSNSETVYLNEEEKLELIRITVEAARKDRLILAGTGMESTRETIKLTNKAARLGAH
                     GALVLTPFYYGGKMNDEALIRYFTEVADNSDIPILIYNVTKFTHVNISANAVEVLSRN
                     PNIIGMKDSSGDVPQLVKFISVVPEDFNLMVGTASSWYPALTLGISAGIMALANCSPN
                     ECASVQEYYENGQIEKAKDTYTRIFPVNTAVTATYGIAGLKYACSLSGYEGGYVRKPL
                     LELKEDEKKKVRDILSKAGLI"
     gene            complement(3050..3655)
                     /locus_tag="PKI50_09785"
     CDS             complement(3050..3655)
                     /locus_tag="PKI50_09785"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNT04031.1"
                     /translation="MEMDFTKLVNRKTAICDKEVYLEFSVLVPIIKTGSEFSLLFEVR
                     SEKLAKQPNEICFPGGKIESFENARQAALRETTEELLIPEANIDVIAELDTIVTPFNR
                     ILYPFAGKIHEYNGTFNSEEVKETFIVPLSFFLENAPLHHDINISMKPVEGFPYHMVR
                     DGRDYPWGKGKYPVYFYTYENKIIWGLTARIIKNFVNLLGS"
     gene            3894..4970
                     /locus_tag="PKI50_09790"
     CDS             3894..4970
                     /locus_tag="PKI50_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNT04032.1"
                     /translation="MHKKLFIPGPIDVREDVLQKMATPQIGHRTKEASALQRSVSEKL
                     MKVFQTKNQIILSTSSGSGLMEGAIRSCTRKRAAVFSVGAFGDRWFKMAKANGVPADK
                     ISSVPGKPTTPEMVDEALATGKYDVITVTQNETSAGIMNPCGEIGELLKKKYPDVLYL
                     IDTVSSMGGTDIRVDEWGADVCITSTQKCLGLPAGMSACSISERAVEAARQVENRGMY
                     FDLVELYDFIQKKDHQYPSTPSLAHMFAMDYQLDKILAEGLEKRFARHIEMAEYVRAW
                     ARKNFALFVEDEKYLSNTLTTVTNTIGIDVGGLNKKLGERGFMISNGYGDLKDKTFRI
                     SHMGDYTLGEVKELIKNINEILGL"
     gene            5015..5926
                     /locus_tag="PKI50_09795"
     CDS             5015..5926
                     /locus_tag="PKI50_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNT04033.1"
                     /translation="MKRILVTDGMEKSAVEKLKNSGYEVVEQFYEPEVLKEQIKNFDA
                     VVVRSATKVRQPIIDAALETGRLKLIIRGGVGVDNIDVDYAKLNGIAVANTPNASSAS
                     VAELAIGHMFCLARHIYIANHTMRQGKWNKKQYEGIELSGKTLGLVGFGRIAREVAKR
                     AFALGMKVVYTNTSGAKEGFNDYKYMKLDELLAVSDFISMHIPCSQDQQPVIGEAQFA
                     KMKDGVYLVNTARGGVVCEEALLKALDSGKVAAAALDVFEEEPTKNERIYTHERISLT
                     PHIGASTNEAQERIGGEIIQIIQNFFE"
     gene            5938..7182
                     /locus_tag="PKI50_09800"
     CDS             5938..7182
                     /locus_tag="PKI50_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNT04034.1"
                     /translation="MATVKAFRAIRPAPELAHKVAALPYDVMDSNEARGMVKGNPYSF
                     LHVDKAEIDLDPSIDLYDKRVYEKARDNLREMIKNGVFQQDEKNCLYIYKQVMKGRVQ
                     TGLVGCTSIDDYMNNIIKKHEYTRADKEQDRINHVDYCNANTGPIFLTYRASKEINEI
                     VDNWMADHKPVYDFVSDDGIGHTVWVIDGDDAIQRLEERFRAVDYLYIADGHHRSASA
                     VKVGLKRRESFPDYKGDEEFNFFLSVLFPDEQLYIMDYNRVVKDLNGNSAEEFLKKVG
                     EKFDIEEYTGEGQFRPTVRHTFGMYMDGKWYKLTAKAGTYNEEDPVDRLDVSIMQNNL
                     LDPVLGIKDPRTDKRIDFVGGIRGLGELERRVNAGMKVAFSMCPTTIMDLMSIADAGK
                     VMPPKSTWFEPKLRSGLFIHEL"
     gene            7430..9289
                     /locus_tag="PKI50_09805"
     CDS             7430..9289
                     /locus_tag="PKI50_09805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNT04035.1"
                     /translation="MNADVFLYFIFITAKLLFFNSIIDGTGMGSIPLIASVAGTVLLL
                     FFWAGLLPGKIRLPLLFMLDCAVTFIIMADLLFFRYFSDVLSMPVLEQASVVTSVKSS
                     ILNLLHASDLIFAADLLFLPFVSAKAGRLNKKPKAGFGKRIICALSALAIGIGLCGCG
                     VSEILKAQPEIFRSFYDRVYIVQNIGLLGFHGIDAFRYVKSKDKLELSLSEDIKKEIA
                     EFIKDKKPEQPSSSKLFGTGKGKNLIVVQVEALQEFVIGKSIEGQEITPNLNKLIKSS
                     LYFDNYYTQTAGGGTSDAELLSNVSMHPARDGATYIRFPLNDYYSLARRLSEEGYLTS
                     AMHAYRPGYWNRSVMYKTLGFDEFIDKEDYIPDEISGMGLTDKSFFRQSLEFMEKYKE
                     PYYTFLITLTSHFPYDNDKKYLGSFNAGKYKGTFFGSYLEVIHYADEALGGFIDELEA
                     RGILDRSVLAVYGDHFGIPRDKKEYLADFLGLKEMDDYNWVKQQRIPLIIRIPEGKYT
                     GTKHIAGGGLDFMPTILNIMGVDSSNIPMLGRDLLNSEEGMAVLRHGYFITDKYICLT
                     SKGVAYDAETGSTYPAEKLAKEKEFAARQLRYSDLITEYNLVKELMEYLKDET"
     gene            complement(9347..10639)
                     /gene="nagZ"
                     /locus_tag="PKI50_09810"
     CDS             complement(9347..10639)
                     /gene="nagZ"
                     /locus_tag="PKI50_09810"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNT04036.1"
                     /translation="MKRLFSIVIVVLILLIAASCTVPDVSEGNTPPVPPAQSDPEAED
                     RKDPLEAKAESLLAGMTIEEKAGQLLIVGFPGDTKKEALQDYIDRLKVSGFILFSRNY
                     TDFDSLYALARSLKEMNSLNNPLPLFISIDEEGGTVSRLPKGGTRFPDARKVGKAGEP
                     GLTYKAGQTIAKELKAAGINLNFAPVLDIVESGENKLLIKRSYGSTPEVVSLHGTSFI
                     SGLQSEGVTAVPKHFPGHGNTSQDSHSTLPVIDTDKAAMQSRELVPFKAAIDAGLDAV
                     MVGHIAFPKLDPTGLPASMSSYFLTDVLRKDLGFGGISISDDIEMQGYISSKDTVEEC
                     VISSFNAGLDIFLIGHTKAIQDQVYKALLDGCRDGRISEERLNESVLRIIKAKLKNGL
                     TDAMEYEIEEAKRIFGSDEHKAILEELNGDTGTFLLSR"
     gene            10836..12080
                     /locus_tag="PKI50_09815"
     CDS             10836..12080
                     /locus_tag="PKI50_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT04037.1"
                     /translation="MDSRNHSLADFFDVDADMAGRAKMFGEYLKDIREKRHLQYRRIS
                     TDGSGPVMKVVDHYTGKIKEMVYLASNDYLNLTKHPRTIAAGRKALEKYGSGAGSVPL
                     LGGTLDIHIELEKKIAKFKGCEDSIIYTSGFGSNCGSLLSILQEKDIAILDTYVHASI
                     IDGCKSTNIRFFRHNDMNSLERVLQRVKDKYRTKLIAVDGVYSMDGDIAPLDRIAELA
                     KTHGAYVMVDEAHATGVIGDTGRGTPQHHKIEGKVDIVAGTLSKALGGVGGFIAANSE
                     LVELLRYYSRPYIFSTAMTPQVAGSLLAAIDVIEEEPEHRERLWENIRYFRSNLTALG
                     FDLGNSETAIFPIIIGNDLKVKEICRRLHELDIYVNPVMYPAVPKRLSRIRMSLMSAH
                     TKEHLDKALDALETAGREYGVI"
     gene            complement(12103..13413)
                     /locus_tag="PKI50_09820"
     CDS             complement(12103..13413)
                     /locus_tag="PKI50_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04038.1"
                     /translation="MENVSNKNALKVHRCIISTGSSSNSYLTRYASDHVEESISICNC
                     LDKEDIPKITPASHYDIIISYSDETIEEIIYDLFSYLIPQDVTIRAAYILDNSMSGSS
                     MPKHVAFLEKLEKHKFITLEFIDDSPEVPVEQEENIIYFVGPGNTGKTSIIASLSESF
                     RQNGQKLALIDITRNNKLINYFPKHSFLNTGNLKDFSISRDFYRSVNIYTDGLVDLYV
                     YDYEYDKKGPEVIYFCEILRKLSGLYDYIIVNADESSVINTPYIYKTASKVFIVHDFM
                     PTKIRTTKKLLLKMISAGINTQKTVSLIYNKTLKDTIDIGRIEEKLLFIRLPNRKILP
                     TVNINCSTFEIPYEQRTMAALVNNLAQNTTGIENTSKKYRHIIQNIYKYINNIPYTEN
                     DEMEISQFLREKLRKLSDRKFMLEYMRNICDTSLIKNYIKAYKK"
     gene            13588..14727
                     /locus_tag="PKI50_09825"
     CDS             13588..14727
                     /locus_tag="PKI50_09825"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HNT04039.1"
                     /translation="MESRVKIYADGGNRLTKIMEESRKPFDFPTIISEPEKELNYGLN
                     FDFDLSDWNNDAAKLDINNMFVEIIKDGVSMGKMLVGKAAEEKGLLIRDRNRYQEKAA
                     DDVIMFCTIAGIAYNLAKYNKNYQKIDLTVNQPLIEYARNKEASANKYTENLMGNMKV
                     LFFNIFNPSQVIYEVIFDIEKVVLCPEGIAAFFQYAVNEDGSLKEEFKNDMKTIVWDI
                     GSGQINVAAFDGFKVAGVNTFEKGMFDCYEKISLILYNNFRDKLDRKPYSYEVDNMIR
                     FNESVLKGKKGEDIDFSNIVKNVFDVFAYDLSKDIREFSKIKFMGACDSIIFAGGGSK
                     ILFDYLSKYLSNDYICVKSNIGEYDNVVGSMYYRMYRDVRSSIEG"
     gene            14739..15545
                     /locus_tag="PKI50_09830"
     CDS             14739..15545
                     /locus_tag="PKI50_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04040.1"
                     /translation="MIIKLLEQPIVAIHEAEIAGTVDGVIIKGSKVNCIYHGNMEDHF
                     IIPIERAIIGSDAVMIPDAAVMVKASKNVRPLRSMVNVYNLKGKCLGYLQGIEADEQF
                     VARYVYTEEYRIELSKAVSYDSVLIVDAEEEDLEAIEEKQDTLKDTENEEAEAAGASG
                     GNLAEGAEAAVSMEIGPEINWDAEDYKPEEKSAESSELSLVKPVHHEEDKKEIPYVEA
                     KYAYLCGKQLLEGIDIDDTFYKKGTIIDADLIKNAISSNAIVKVIVNAEE"
BASE COUNT         4598 a         3090 c         3791 g         4088 t
ORIGIN      
        1 atgaaaggaa ggtgatgccc gcatgaataa agacaaagca gccaatagac tgattactga
       61 aaaatcccct tacctgctgc agcatgctta taacccagtt gactggtatc cttggggaca
      121 ggaagcattt gataaggcaa aggcagaaga taagcctgtt tttcttagtt taggttatag
      181 tacctgccat tggtgccatg tgatggagcg ggaatccttt gaggataagg aagttgcgga
      241 gatattaaac aaaaactaca tagccattaa ggtagacagg gaggagaggc ctgatatcga
      301 ccatatttac atgaatgtgt gccaggcttt gacgggaagc ggcggatggc ccctgaccat
      361 aataatgact ccggaaaagg ccccattttt tgcaggcaca tatttcccca agagggcaag
      421 gatgggctat ccgggagtga ttgaattact ggagaaaatc aatgaggcct ggaatacgga
      481 gagggaaaaa ttattggagg cctcggagaa tatattggag catatcagat ccgattctgc
      541 tgctgattct gcggttgagc ttgacattga aactgcaggc aaagcattca aaggtctgga
      601 aaggagcttc gacagtgaat atggggggtt cgggagagca ccaaagttcc cgacgcctca
      661 taatctgcgg tttttgctga ggtactggaa ggtgacaaat gaccccaaag ccttgcagat
      721 ggttgaaaag actctgattt caatgtaccg gggaggcatc tttgaccatg tgggttatgg
      781 gttttccaga tactccaccg accgcatttg gctggtgccg cattttgaaa agatgcttta
      841 cgataatgca ggtttggctg ccgcatacct ggaagcatac caggccaccg gaaaggagca
      901 gtacggggag gttgcaaggc agatattcga gtacgtgctc agggatatga cttcaccgga
      961 gggagccttt tactctgcgg aggatgccga ttcggaaggg gtggaaggga agtactacgt
     1021 atggagccca aaggagataa cgaacatttt gggcgaggac gctgcagtgg agttcaacgg
     1081 ctattacggg ttaagcgacg atggaaattt tgaggggaaa aacatcccca accttctgca
     1141 taataaagct gcttttgaaa atatccggaa aaaccccttg gaaggtcata ggaaaaaggt
     1201 atttgaagca agagaaaaga ggattcatcc ctataaggac gacaaggtac tgacagcatg
     1261 gaacggcttg atgatagcag ccctatccat tggagcaagg gtgctggata acatggaata
     1321 tgcggttgca gctgaaaggg ctgtaaggtt tatcggatca aagcttatac gggaggacgg
     1381 aaggctgctt gcaagatata gagaaggaga agccgcttat ccgggatacc tggatgacta
     1441 tgcatttctg gtatgggggc ttatagagct gtatgaagca accttcaggg ccgagtattt
     1501 gaagctggct gcagacttga accgcgatat gctgaagtat ttctatgacg aggaaaaggg
     1561 agggcttttt ctttacggaa gcgacagcga gcagctgatt gtcaggccta aggatgtata
     1621 tgacggtgca atgccttcgg gcaattcggt tgccgccatg aattttttga ggcttggaag
     1681 gcttacggga gatactgatt tggaagacaa ggcttataag cagcttgaag ctttcggaag
     1741 tattataaaa ggccacccga tgggatatgc cgatatgctg tcggctttgc tttttattaa
     1801 cagcaaagca agtgaaatag tgatagtagg caatcgtgag gatgagaatg caaaaagaat
     1861 gataaaaagt ataaatagcc atttcagccc atttaccgtt gtcctgttca aggataaaga
     1921 aaatgcggaa gaaggtatta caagcattgc cccttatact gaagctcaag ggatggtaga
     1981 taacaaaaca actgcctatg tatgcgaaaa cttcgcctgc agggctccta taacggattt
     2041 gcaggagttt gaaaatgttt tagcatgagc agaggatatg gataaaggga cagagacacg
     2101 gtacctgtcc ccatgtccct aaataagtcc ggctttggac agaatatccc ttactttttt
     2161 cttttcatct tcttttaatt ccagcagggg cttccttaca taaccccctt catatcctga
     2221 cagggagcat gcgtatttca ggccggcaat tccataggtg gctgtaaccg ctgtgtttac
     2281 tggaaatatc ctggtatatg tgtcttttgc tttttcaatt tggccatttt catagtattc
     2341 ctgaaccgat gcgcattcat tgggtgaaca atttgccaat gccataattc ccgcacttat
     2401 gcccaaggta agagccggat accatgaaga tgccgttcca accatcaggt tgaaatcctc
     2461 aggtacaacg ctaataaatt ttaccaactg gggtacatct ccggaactgt ccttcatgcc
     2521 gattatgttt ggattcctgc ttaatacctc tactgcattg gcagaaatat tcacatgtgt
     2581 gaatttggtc acattgtata tcaatattgg gatatccgaa ttgtctgcga cttcggtaaa
     2641 atacctgatg agagcttcat cattcatttt accgccgtaa taaaaaggag tcagaaccaa
     2701 tgcaccgtga gccccaagcc tcgcagcctt attagtcagc tttattgttt cccttgttga
     2761 ttccatgccg gttcccgcaa gtataagcct gtcttttctt gccgcttcaa cagtaatcct
     2821 tatcagctca agtttttctt cctcattcag gtatacggtt tcactattgg atcccaggac
     2881 caggcagcct gccaggttat cattgttcca tttctccata ttaccggcat gcatccgata
     2941 atccacatcc ccgttttctt tgaagggggt gatcattgga ggtataacac ctttgattct
     3001 tttaatttcc atgctgttga tactccttct tttgtattcc gggtacatat tatgaaccca
     3061 gcagatttac aaagtttttg ataatccttg cagtcagtcc ccatattatc ttattttcat
     3121 aggtatagaa atagacaggg tatttccctt tcccccaggg gtaatccctt ccgtcccgca
     3181 ccatatgata tggaaaacct tccacaggtt tcatacttat attaatatca tgatgcaacg
     3241 gggcattttc caggaagaag gaaaggggaa caatgaaggt ctcctttact tcctcagaat
     3301 taaaggttcc gttgtattca tggatttttc cggcgaaggg gtacaggatt ctgttaaatg
     3361 gtgtgacaat agtgtccagt tcggcaatga catcaatgtt cgcttcagga atcagaagct
     3421 cctccgtggt ttccctcaag gcagcctgcc gtgcattttc aaagctttct atcttccctc
     3481 cagggaagca tatttcgtta ggctgcttgg caagcttctc cgatctgact tcaaaaagaa
     3541 gtgaaaactc actgccggtt tttattatcg gaaccagtac ggagaattcc agatatactt
     3601 ctttatcgca tatggcggtt ttcctgttga ccagttttgt aaaatccatt tccatatgac
     3661 acctctttgc aatgatatgg tgaagggata ttctcatata tcaattataa caaatgccgc
     3721 tgaatatttg aatacttcaa attggccatc tgtgcccgga ttgaggacac cgggcaaaaa
     3781 atgtctttaa ttaatttaaa tgtgttgagg cttattccca taaatggtat tattatcttg
     3841 ggttataaat tattaaatta taattttaga aaagagttaa gggaggaacg aaaatgcaca
     3901 aaaaattatt tattccaggg cctatcgatg tacgtgagga tgtgcttcaa aaaatggcta
     3961 cacctcagat agggcatagg acaaaggagg catcggcatt acagcgaagt gtttctgaaa
     4021 agcttatgaa agtatttcag accaagaacc agataatact ctccacatcc tccggaagcg
     4081 gcctgatgga aggtgccatc cgctcctgca ccaggaaaag agcagcggta ttttcagtag
     4141 gtgcctttgg agacagatgg ttcaagatgg ccaaagccaa cggggtaccg gcggataaaa
     4201 tctcatcggt accgggaaag cctactactc ctgaaatggt ggatgaagca cttgccaccg
     4261 gcaaatacga tgtaatcaca gtaacccaga atgaaacctc ggcaggcatt atgaatccct
     4321 gcggagaaat cggagagctt cttaagaaga aatatccgga tgtgctttat cttatagata
     4381 ccgtaagctc catgggagga acagacatca gagttgacga gtggggagcg gacgtctgca
     4441 ttacatcaac acagaaatgc cttggcttgc cggcaggcat gtctgcatgc tcgatatccg
     4501 aaagggctgt tgaagccgcc agacaggttg agaacagggg gatgtacttt gacctggtag
     4561 agctttatga tttcatacag aaaaaggatc atcaatatcc ttcgactccg tcccttgccc
     4621 acatgtttgc aatggactac cagttggata agattcttgc cgaaggcctt gagaagagat
     4681 ttgccagaca tatcgagatg gctgaatatg tacgtgcatg ggcaagaaag aactttgcgc
     4741 tgtttgtgga agatgagaaa tacctttcaa atacccttac taccgttacg aatacaatag
     4801 gaattgatgt cggcggcctg aacaaaaagc tgggtgagag aggctttatg atttcaaacg
     4861 gttatggaga tcttaaggac aagaccttca gaatatccca tatgggtgat tacactcttg
     4921 gtgaagtgaa ggagcttatt aagaatatca atgagatttt gggactgtaa gagggacttt
     4981 cagaaaacaa ataaagcttg ggggttgaaa acagatgaaa aggatacttg taactgacgg
     5041 tatggaaaaa tccgctgttg aaaagcttaa gaacagcggc tatgaggtgg ttgaacaatt
     5101 ttacgagccg gaagtgttaa aggagcaaat aaaaaacttt gatgcagttg ttgtgcgttc
     5161 ggcaacaaaa gtaaggcagc cgataataga tgcagctctg gaaacaggaa ggcttaagct
     5221 cataatccgc ggcggcgttg gagttgataa tatagatgtg gattatgcaa agcttaacgg
     5281 tatagcagtt gccaatacgc ccaatgcaag cagcgcatcg gtggcggaac tggctatagg
     5341 ccatatgttc tgcctggcaa ggcatatata tattgccaat cataccatga ggcaaggcaa
     5401 atggaacaag aagcagtacg aggggattga gctttccggc aaaaccctgg gccttgtagg
     5461 ctttggaaga attgcaaggg aggttgcaaa aagggcattc gctttgggaa tgaaggttgt
     5521 ttataccaat accagcggag caaaggaagg cttcaacgat tacaaatata tgaaactgga
     5581 cgaacttctg gctgtctccg attttatatc gatgcatata ccctgctcac aggaccagca
     5641 gcctgtaata ggtgaagcgc agtttgcaaa aatgaaggac ggagtatatc tggtcaatac
     5701 ggcaagaggc ggtgtcgtat gcgaggaagc gctcttgaag gctcttgact ccggaaaggt
     5761 tgccgccgcc gctcttgatg tatttgaaga ggaacccacc aagaatgaaa gaatatacac
     5821 tcatgagaga atatcattga cgccccatat aggtgcttca accaatgagg ctcaggaaag
     5881 aataggcggc gaaataatac agattattca aaatttcttt gaataggaga gaaaactatg
     5941 gcaacagtaa aagcattcag ggctataagg ccggcaccgg agctggcgca caaggtggca
     6001 gcccttcctt acgatgtaat ggacagcaat gaagcaagag gaatggtaaa aggcaatccc
     6061 tattcattcc tgcatgttga caaggcagaa atagaccttg acccttccat tgatctttat
     6121 gacaaaaggg tatatgaaaa agccagggac aatctgagag aaatgattaa gaacggagtt
     6181 tttcagcagg atgaaaagaa ctgcctgtac atatacaagc aggttatgaa ggggcgtgta
     6241 cagacagggc ttgtgggctg cacctcaata gatgattata tgaacaacat tatcaagaag
     6301 cacgagtata caagggcgga caaggagcag gacaggataa accatgttga ttactgcaac
     6361 gccaataccg ggccgatttt ccttacatac agggcttcaa aggaaataaa tgaaatagtg
     6421 gacaactgga tggctgacca caagccggta tatgactttg tatccgatga cggtataggc
     6481 catacggtat gggtaattga cggggatgat gcaattcaga ggctggaaga aaggttccga
     6541 gccgttgact atttatacat agccgacggg caccaccgtt ccgcatccgc ggtaaaggta
     6601 ggcctcaagc ggagagagag cttcccggac tacaaggggg acgaggaatt caacttcttc
     6661 ctctcggtac ttttccccga tgagcagctt tatataatgg attacaacag agtggtaaag
     6721 gacttgaacg gcaattccgc tgaagagttc ctgaaaaaag tcggagagaa atttgacatt
     6781 gaagaataca ctggggaagg acaattcaga cccactgtca ggcacacctt cgggatgtac
     6841 atggatggaa agtggtataa gcttacggcc aaggcaggca cttacaatga agaggatccg
     6901 gttgacaggc tggatgtgtc aattatgcag aataacctgc ttgaccctgt attaggaata
     6961 aaggatccca gaaccgacaa gagaatagac tttgtaggag gtatcagagg cctcggggaa
     7021 ttggaaagaa gggtaaatgc cggtatgaaa gttgctttct ccatgtgccc taccactatt
     7081 atggacttaa tgtccatagc cgatgcagga aaggttatgc cgcccaaatc aacctggttt
     7141 gagccaaagc taagaagcgg attattcata cacgaactat agaaaaacac ccgattcatt
     7201 tccctgatgc caacaggcac ttcagggcag cggcaagcgg tgttaaggaa atgctgtaaa
     7261 aggatatcgc aaaactactt gataggtagt tggcgatatc cctcttcttg tataagtgca
     7321 gccctcattg cttaagcaca gcttgtataa ttctcttgat tgctgtagaa tatccttgtt
     7381 gaaatagtat gaatacataa caaaatgcag ttaatcagag gatgatacaa tgaatgcgga
     7441 tgttttcctt tattttattt tcataacggc aaagctcctg ttttttaaca gcataataga
     7501 tgggacaggc atgggaagca ttcctttaat tgcttccgtt gccggaacgg tattgctgct
     7561 gtttttctgg gccggcctgc tgccggggaa aatccggctg ccgcttcttt ttatgcttga
     7621 ctgtgccgta acattcataa taatggcgga tctattgttt ttcaggtatt tcagcgacgt
     7681 gctttcaatg cccgtattgg agcaggcttc ggttgtcact tcggtgaaaa gcagcattct
     7741 gaaccttttg catgcatccg accttatatt tgccgccgat cttctgtttt tgccttttgt
     7801 atcggccaag gcaggcaggt tgaataaaaa acccaaggca ggcttcggaa agagaatcat
     7861 ctgcgccttg tcagcccttg ccataggtat cgggctgtgc ggatgcgggg tgtcggaaat
     7921 actgaaggcg cagcccgaaa tattcaggtc attttatgac agggtatata ttgttcagaa
     7981 tatagggctg ctgggctttc acggcataga tgccttccgg tatgtaaaat caaaggataa
     8041 attggaactg tccctatcgg aggatataaa gaaggaaata gctgaattca ttaaggataa
     8101 aaagcctgaa caaccgtcat catccaagct tttcggaacc ggaaaaggca agaatcttat
     8161 agtggttcag gtggaagcac tgcaggaatt tgtcatagga aagagcatag agggtcagga
     8221 gattaccccg aatctgaaca aattgataaa aagcagctta tattttgata actattatac
     8281 acagacggca ggagggggaa catccgatgc ggagcttctg tccaacgtat cgatgcatcc
     8341 tgcgagagac ggagctacat acatcagatt cccgctgaac gattattact cacttgccag
     8401 gaggctttcg gaagagggct atttgacctc tgcaatgcac gcctacagac ccggctactg
     8461 gaacaggagc gtgatgtaca agacccttgg ctttgatgag tttattgaca aggaggatta
     8521 tattcccgat gagatcagcg gaatggggct taccgacaaa tccttcttca ggcagtctct
     8581 tgaatttatg gagaaataca aggaacccta ctatactttt ttaataactc tgaccagtca
     8641 ttttccttac gacaatgaca aaaaatactt gggcagcttt aatgccggca agtacaaggg
     8701 tactttcttc ggcagctatc ttgaggtcat tcattatgct gacgaagccc ttggagggtt
     8761 catagatgag ttggaggcaa gaggtatatt ggaccgttcg gtattggccg tatatggaga
     8821 ccatttcgga atacccaggg acaaaaagga atacctggcg gacttcctcg gattgaagga
     8881 gatggatgat tacaattggg tgaagcagca gaggatacct ctgattatcc gcattccgga
     8941 gggaaaatat acagggacaa agcatatagc agggggcgga cttgatttca tgccgacaat
     9001 attgaacatc atgggggttg acagcagcaa tataccgatg ctgggcaggg atttgctgaa
     9061 ttctgaagaa ggaatggcag tgctgcgcca tgggtatttt attaccgaca agtacatttg
     9121 cctgacctcc aagggtgttg cttatgatgc ggaaaccggc agcacatacc ctgcggaaaa
     9181 gcttgcaaag gagaaggagt tcgcagccag gcagctccgg tattcggatt tgattaccga
     9241 gtacaatctg gtaaaggaac tgatggaata tctgaaggat gagacatagg acatggggac
     9301 atggggacgt ttcgcgggac acggggacgt ttctcctgtc ccgctttcag cgggacagga
     9361 gaaacgtccc cgtgtcccca ttcagttcct ccagaattgc tttatgctcg tcactgccga
     9421 aaatcctttt tgcctcttca atttcatatt ccatggcatc cgtaagccca ttcttaagct
     9481 tggcctttat tatccggagc accgactcat tgagcctttc ctctgatatt cttccgtccc
     9541 ggcatccatc gagtaaagct ttatatacct gatcctgaat ggcctttgtg tgccctatca
     9601 aaaaaatatc aaggcctgcg ttaaaggaag agattacaca ttcctcaaca gtatccttac
     9661 tgcttatgta cccctgcatc tcaatatcat ccgatatgga aattcctcca aagcccaaat
     9721 cttttctcag aacatcggta agaaaataac ttgacatgga agccggaagg cctgtagggt
     9781 caagcttggg aaaagcaata tgcccgacca ttacggcatc cagtcccgca tcaatggcgg
     9841 ccttgaaagg aaccagctct ctgctttgca tcgcagcttt gtccgtatca ataaccggaa
     9901 gagtactatg agaatcctga ctggtgtttc catgtcccgg gaaatgcttc ggcactgctg
     9961 ttacgccttc ggattgcagt ccgctgataa atgatgttcc gtgcagcgaa accacttccg
    10021 gagtgcttcc gtaagacctc tttataagca gcttgttctc accgctttcg acaatatcaa
    10081 gaaccggggc aaaattaaga tttattcctg cggctttaag ctcttttgct atagtctgcc
    10141 ctgccttata tgtgagcccg ggctctcccg cttttcccac cttccgggca tcggggaaac
    10201 gtgtaccgcc ctttgggagc cgtgatacgg tgcccccctc ttcatcaatt gaaataaaca
    10261 gcggaagagg attattaagg gaattcattt ccttcaggct cctggccagt gcatacaggg
    10321 aatcaaaatc cgtatagttc ctgctgaaaa gaataaagcc ggataccttt aacctatcta
    10381 tataatcctg caaagcttct ttttttgtat ctccagggaa ccccacaatc aacagctgtc
    10441 ctgctttttc ctcaatagtc atcccggcca gtaaggattc tgcctttgcc tcaagaggat
    10501 ccttcctatc ctctgcctca gggtcactct gagccggggg aacgggagga gtgtttcctt
    10561 ccgaaacatc aggaacggtg cagcttgctg ctattaataa aataagcacg acaatcacaa
    10621 tactgaatag ccttttcaac ggtttatgct ccttttcttg tatatactcc aattatcacc
    10681 ggcaggacag catatgtcaa tatatgaaaa gcccgccgga ttagatgaat tgcacttcta
    10741 aatattagaa aaacaggcat aatatatata ataatgtgca cagaaatgta cacatccgat
    10801 taaccgcaga ataaaaacca ataggaggta aggatatgga ttcaaggaat cacagtctgg
    10861 cggatttttt tgatgtcgat gccgatatgg caggcagggc aaagatgttc ggagaatatc
    10921 tgaaggatat cagagagaaa aggcatcttc aatacagaag aatatcaacg gatgggtcag
    10981 ggccggtaat gaaggttgtt gaccattata caggaaagat taaggaaatg gtgtacctgg
    11041 cgtccaatga ctatctcaat cttaccaagc atcccagaac catagcagcc ggaaggaaag
    11101 cgctggagaa atacggttcc ggtgccggaa gtgttcccct gttgggagga accttggaca
    11161 tacatattga actggagaaa aagatagcaa aattcaaagg ctgtgaggat tcaatcatct
    11221 atacctccgg cttcggctcc aactgcggga gcctgctttc catacttcag gagaaggata
    11281 ttgcaatact tgacacctat gttcatgcaa gtataataga cggctgcaaa tcaacgaata
    11341 taaggttttt caggcataac gacatgaact cccttgaaag ggtgcttcaa agagtgaagg
    11401 ataagtatag aacaaagctt attgcagttg acggagtata ttcaatggac ggggatatcg
    11461 ctccccttga caggatagct gagctggcaa aaacccatgg ggcatatgta atggtggatg
    11521 aagcccatgc aacaggggta ataggagata ccggaagggg gacccctcag catcacaaaa
    11581 ttgaaggaaa ggttgatatt gttgccggca ccctgtcaaa agccttggga ggggtgggag
    11641 gcttcatagc tgcaaattcg gaactggtag agctgctgcg ctactattca aggccgtaca
    11701 tattttccac ggcaatgact cctcaggtgg caggctcgct gctggcagca atagatgtga
    11761 tagaggagga accggagcac agggaaaggc tttgggagaa cataaggtat ttcaggtcta
    11821 atctgaccgc cctggggttt gacttgggga attcggagac ggcaattttc ccgattatca
    11881 taggaaatga ccttaaggta aaggaaatct gcagaaggct ccatgaattg gacatatatg
    11941 tgaatccggt catgtacccg gctgttccca aaagattatc aagaatcaga atgagcctca
    12001 tgtccgccca tacgaaagaa cacctggata aagccctgga tgccttggaa acggcaggaa
    12061 gggaatatgg cgtaatatga aaatagtaca tcgggagatg tactattttt tatacgcctt
    12121 aatgtaattc tttatcagtg aggtatcgca gatatttctc atgtattcca gcataaactt
    12181 cctgtcggaa agcttccgga gcttttccct gaggaactgg gagatttcca tttcatcatt
    12241 ttcggtatag ggaatgttat ttatgtattt ataaatattt tgaataatat gcctgtattt
    12301 tttggaggtg ttttctatgc cggtggtgtt ttgggcaaga ttatttacta atgcagccat
    12361 tgttctctgc tcatagggta tctcaaaggt actgcagttg atgttcacgg taggcagtat
    12421 cttcctgttg ggcagcctga tgaaaagcag cttttcttct atccggccta tatctatggt
    12481 atccttaaga gtcttgttat atataaggga tacggtcttc tgcgtgttga tgcctgcgga
    12541 tatcattttc aaaaggagtt tttttgtagt tcttattttt gtaggcatga agtcatgtac
    12601 aataaaaacc ttgctggctg tcttatatat gtatggagtg tttattaccg aactttcgtc
    12661 agcattgaca atgatgtaat cgtacaagcc gctcagcttt ctgagtattt cgcagaaata
    12721 aattacctca ggcccttttt tgtcgtactc gtagtcatat acatacaaat ctacaagccc
    12781 gtcggtataa atgttcacag acctgtaaaa atccctggat atggaaaaat ccttcaaatt
    12841 gccggtgttg agaaagctat gctttgggaa atagttgata agcttattat ttctggttat
    12901 atctataagt gccagcttct ggccgttctg cctgaagctt tcgctgaggg acgctataat
    12961 tgaggttttg ccggtattgc cgggccctac gaaatatatt atgttttctt cctgttcaac
    13021 gggcacttca gggctgtcat caataaactc aagagtaatg aatttgtgct tttcaagctt
    13081 ttccaaaaat gctacatgct tcggcatgct gcttccggac atgctgttgt ccaatatata
    13141 tgcagccctg atggtaacat cctggggaat caaataactg aacaggtcat atatgatttc
    13201 ttctattgtt tcatcggaat aactgatgat tatatcataa tgactggccg gggttatctt
    13261 gggtatgtct tccttatcca aacaattgca gatggatatg ctttcttcga catgatctga
    13321 ggcataacgg gtaaggtaag aattagagga tgaacctgtg gaaatgatgc atcgatgcac
    13381 ctttaaagca tttttattac ttacgttctc cactctatcc ccccctttga taaagctgca
    13441 cacaccttga aattaacaca aaatggctat tttttcaaca caatcagaca aattaggact
    13501 aaagtcctat atgatgctgg gaagatatgg agtatcgtat attatagagt agttgtattc
    13561 atttttaata ttgaaggagg tcttttaatg gagtctcgag ttaaaattta tgcagatggt
    13621 ggaaatcgat tgacgaaaat tatggaggaa tccagaaagc cttttgattt tccaactatt
    13681 atatctgagc cggagaaaga attgaattat ggattgaact ttgacttcga cctttcagac
    13741 tggaataacg atgccgcaaa actcgatatt aacaatatgt ttgttgaaat catcaaagac
    13801 ggcgtatcaa tgggcaagat gctggtcggt aaggccgccg aagaaaaagg acttttaata
    13861 agggatagaa acagatacca ggaaaaggca gcagatgatg tgataatgtt ctgcacaatt
    13921 gcgggcattg catataatct ggctaaatac aacaagaact atcaaaaaat agaccttaca
    13981 gtcaaccagc ctcttatcga gtatgcaaga aataaagaag catctgcaaa caaatataca
    14041 gagaacctta tgggcaacat gaaggtatta ttctttaata tattcaaccc ttcccaggta
    14101 atatacgaag taatattcga cattgaaaag gttgtattgt gccccgaagg aatagcagca
    14161 ttcttccagt atgcggtcaa tgaagacgga agcctgaagg aagaattcaa gaatgatatg
    14221 aagacaatag tttgggatat tggttccggt cagataaatg tagctgcatt tgacggtttc
    14281 aaggtagcgg gggtcaacac ctttgaaaaa ggtatgtttg actgctacga aaaaatctcc
    14341 ctcattctgt acaataactt cagggacaag ctggacagaa agccatacag ttacgaagta
    14401 gacaacatga taagattcaa cgagtcggtg cttaagggta aaaagggaga agatattgat
    14461 ttcagcaaca tagttaaaaa cgtatttgat gtttttgcat atgatctcag caaggatatc
    14521 agagaatttt ccaaaataaa gtttatgggc gcctgtgaca gcataatatt tgccggcggc
    14581 ggaagcaaga tacttttcga ttatctcagc aagtatctca gcaatgatta tatttgtgtt
    14641 aagagcaaca tcggagagta tgacaacgtg gtaggaagta tgtattacag gatgtacagg
    14701 gatgtaaggt caagtattga agggtaggtg ccggcttttt gattattaaa ctgttggaac
    14761 aaccaattgt agcaattcac gaggctgaaa tcgcaggtac ggttgacgga gtgataatca
    14821 aaggcagcaa ggtcaactgc atatatcacg gcaatatgga ggatcatttt atcattccga
    14881 ttgaaagagc aattatcggc agtgatgccg tcatgattcc ggacgcagca gtcatggtca
    14941 aggcttcaaa gaatgtaagg cctctcaggt cgatggtaaa tgtgtacaat ctcaagggaa
    15001 agtgcctggg gtatctgcag ggaatagagg cagacgaaca atttgttgcc cgttacgtat
    15061 acacagagga atacaggatt gaactgtcaa aggcagtgag ctatgacagc gtcttaatcg
    15121 tagatgcaga agaagaagac cttgaggcaa ttgaagaaaa gcaagacact ctgaaggata
    15181 ctgaaaatga agaagctgag gctgcaggag cttccggggg taacttggca gaaggcgcag
    15241 aagcagcagt atcaatggag atagggccgg aaatcaattg ggatgctgag gattacaagc
    15301 cggaggagaa gagtgccgaa agctcggaac taagcctggt aaaacccgta caccatgaag
    15361 aggacaaaaa agagattccc tatgtagaag ccaaatatgc atatctgtgc ggcaagcagc
    15421 ttcttgaagg catagatatt gatgatacct tctacaagaa aggaacaata atcgatgctg
    15481 acctgataaa gaatgcaatc agcagcaatg caatcgtaaa ggtaatagta aacgcagagg
    15541 aataggaaga aggcgagaaa tcgcctt
//
LOCUS       DAOQUR010000054        15390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4371_length_15390_cov_11.818846, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000054 DAOQUR010000000
VERSION     DAOQUR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15390
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4371_length_15390_cov_11.818846"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="PKI50_09835"
     CDS             <1..2324
                     /locus_tag="PKI50_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT04041.1"
                     /translation="NRINFRLRTLLYFLIVSIIPFAVSWAFIYDSYYKKMQQDFRSFN
                     HMYIQNQFSKIGQQMYQHEMVLKSVAQAYSFLDPQTADLNAFLRDQNSVNLIFRNLSI
                     IMPDNSVYTGNPGYLKPDINYTLLFSYINARKAQELVWLESYKETLSGVQCVGMSIPL
                     LDNNNKASGVLVGNISLWTFENLLSNAKYLPYAEVFLVNPAGYVKYHSGNKYSEIVNV
                     TDKDFFLSPAADSILKSDEGYEEFSYAGKNWLCSFSSINSAGWKVLSLIDAQMLQSTF
                     GVMNQNINRSIMSSGILCILIGIAASFFMSNSITTPLIKLRDGVKATTEGNLNNRIIV
                     KGDNEIREVADAFNEMASNLNKTYTDLVKRTEELYANNEELHNVNIELEASYGQLGAT
                     VAQLNESDAQLRRKNSELQTLNKISNALNSTMDLSTMLITVVNQVTDITESLACTIRL
                     INEKDPYKLELKAMKGINTEMYDIGTIDIREDVIGRAVKTGSIYIIDLDNEKVPHEYF
                     MRLNKEHDAKCIVFTPIMVKSKVIGVLSITLKQIPSEELIELLNSLTNSIAIALDNAK
                     AYESLKQSYHKTVRSLVSVVEAKDQYTESHSIRVAKYASFIASELNYPKNFIEDIWVA
                     GLLHDIGKIGISDSILNKPGALTKEEYDIIKQHPEIAYKIVSKIGLRENVLKAIKHHH
                     ERCDGKGYPDMIKSGEIPIMASIISVADAFDAITSNRPYRKSRSMKQGIDEIVMHRGT
                     QFDSTVAETLKKVFLTKHEILERIYNDEEIEFF"
     gene            2420..4036
                     /gene="dnaX"
                     /locus_tag="PKI50_09840"
     CDS             2420..4036
                     /gene="dnaX"
                     /locus_tag="PKI50_09840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNT04042.1"
                     /translation="MYTALYRKWRPQRFEDICGQEHITVTLKNELESGRLSHAYLFCG
                     TRGTGKTTTAKLLAKAVNCKASHGIDPCNECESCVSINDGNSIDVYEIDAASNRGIDD
                     IRNLREAIRFTPTLGEYKVYIIDEVHMLTNEAFNALLKTLEEPPEYVLFILATTEPHK
                     LPATILSRCQRFDFKRISSEKIVERLKFVCREEGLEAEEDAVKLIARNSDGAMRDALS
                     ILDQCAVYGSKKITHDDVLEVLGIVNNQYLFRIAEAVLEEDAGTAIKVIDEVAAGGKD
                     ISQFIRDLLVHYRNLLMAKVVTNCEDVIDMSKEGIEQLKKQSGSYDKNNIVRCINCLS
                     ELENEAKWSVNPRILLEVALVKMCRVGEEKSLEGILTRLSKLEERISKGSFDLKAADK
                     PKAAGQEEKREAAVKKEKAAAESKETAVSNPDSGSIIEKWPGFIQEMKVRGKIRLRTY
                     LAMAKPVKAESGIMVLSFEKEGVFSKEALELPANRADVEEAASKYFGAPLKIKCIISG
                     EEEDEQDSIVKAAIEFAGMDKVEVVEEEEE"
     gene            4040..4381
                     /locus_tag="PKI50_09845"
     CDS             4040..4381
                     /locus_tag="PKI50_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNT04043.1"
                     /translation="MGKGGFPGMGGNMNNMMKQVQKMQREMAKLQEEIEGRSVEASAG
                     GGAVTVVANGKKEILSITIKPEVIDPDDAEMLQDLITAAVNEAIRQADEMISREMSKL
                     TGGLNLPGGLF"
     gene            4381..4980
                     /gene="recR"
                     /locus_tag="PKI50_09850"
     CDS             4381..4980
                     /gene="recR"
                     /locus_tag="PKI50_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNT04044.1"
                     /translation="MSFYAAPIAKLIDELSKLPGVGNKTAQRLAFHILNMSSEDVEKL
                     SSSIVSAKRNIRYCKVCCNITDSDLCDICANAKRDSSTICVVEDPKDVVAMEKTREFK
                     GLYHVLNGAISPMEGIGPEEIRIKELLQRVADNEIKEIILATNPNIEGEATAMYISRL
                     LKPIGIKVTRIAHGVPVGGDLEYADEVTLMKALEGRREI"
     gene            5069..5359
                     /locus_tag="PKI50_09855"
     CDS             5069..5359
                     /locus_tag="PKI50_09855"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HNT04045.1"
                     /translation="MSTNLADREPKKNKQIEDIIDCLLNSMMGTRERDENAELINTIL
                     IAKQEMHDAQNYFDNVTAPELVDHAIFRMEAAKSQYVYLLKLAKDKGLSVTI"
     gene            5461..5730
                     /locus_tag="PKI50_09860"
     CDS             5461..5730
                     /locus_tag="PKI50_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HNT04046.1"
                     /translation="MPQIDVFSILAVFFAIGAVYVIGVLLVLPIKIIVKLVMNGIIGA
                     VALFAINIFSGLTGLSIGINPITALVAGFLGIPGIILLLFLQYIL"
     gene            5971..9498
                     /gene="nifJ"
                     /locus_tag="PKI50_09865"
     CDS             5971..9498
                     /gene="nifJ"
                     /locus_tag="PKI50_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNT04047.1"
                     /translation="MAKKMKTMDGNAAAAHVAYAFTEVAAIYPITPSSTMAENVDEWS
                     AHGLKNIFGQQVKVVELQSEGGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAG
                     ELLPGVFHVSARALASHALSIFGDHQDVMATRQTGFALLASGSVQEIMDLGGIAHLAA
                     IKSRVPFVHFFDGFRTSSEVQKVEVMDYADLEKLLDKKALDEFRKNALNPEHPVTRGT
                     AQNPDIYFQAREACNKYYDAVPDIVADYMKEISKLTGREYKPFNYYGAPDAENIIVAM
                     GSVTDAVEETVDYLMAKGEKVGVVKVHLYRPFSEKYFFDVLPKTVKKIAVLDRTKEVG
                     STGEPLYLDVRSMFYNKKNAPLIVGGRFGLGSKDTTPSQIKAVFDNLKTNEPKDGFTI
                     GIVDDVTNLSLEVKENINAAPEGTIRCKFWGLGSDGTVGANKSAIKIIGDKTDLYAQG
                     YFAYDSKKSGGVTISHLRFGKKPIKSPYLIDEADFIACHNQAYVHQYDVIKGLKDGGT
                     FVLNCKWNADELEENLPANMKKYIADHKIKFYIINATDIAVKIGLGNRINMIMQSAFF
                     KLANVIPIEDAVKYLKESLAETYGKKGQKIIEMNYKAVDAGTDALVKVTVPESWSSAK
                     EEAAAAVDVPDFIKNVLIPMNRQEGDTLPVSKFVEYADGHLPMGTAAYEKRGIAVFVP
                     EWQKEKCIQCNQCSFVCPHAAIRPVLVAEDELKKAPAGFETIKASGKGFEGVSYRMQV
                     SPLDCTGCGNCADICPAKEKALVMKPLDTQTEKGIDNWNFAMTVPVKDNLMTKSTIKG
                     SQFAQPYLQFSGACAGCGETPYIKVVTQLFGERMMIANATGCSSIWGASTPSTPYCKD
                     ANGRGPSWGNSLFEDNAEYGFGMAVAVNQIRAKLAEIMTEALALNMPNEYKEAYKAWL
                     DGKDDADASKAAAEKILPLLYNMSALSGRAKEIAAEIADKKDQLVKKSIWILGGDGWA
                     YDIGYGGLDHVLASGENVNVMVFDTEVYSNTGGQSSKATPVGAVAKFTASGKKTKKKD
                     LGMIAATYGYVYVAQVGMGADKNHFLKVIQEAEKYDGPSLIICYAPCINHGLKEGMGR
                     TQANTAQAVEAGYWHLWRYNPELKKEGKNPFILDSKEPKASFRDFLMNQVRYSSLVGA
                     FPEEAEKLFAKAEKEAKEKYENYKKMAEA"
     gene            9619..10197
                     /locus_tag="PKI50_09870"
     CDS             9619..10197
                     /locus_tag="PKI50_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT04048.1"
                     /translation="MGKLIEIRWHGRGGQGAKTAALLLADAAFNTGKYIQGFPEYGPE
                     RMGAPITAYNRISDERIKIHSNIYEPDYVVVVDESLISAIDVTSGLKETGAIIINTDK
                     KPDEVKKHLKGYKGKVCTIDARKISEETIGRNFPNTPMLGAVVVVGNIMEPQKFLEDM
                     KESFAHKFKTKPEIIEGNMKAIERAMQEVQEL"
     gene            10194..10508
                     /locus_tag="PKI50_09875"
     CDS             10194..10508
                     /locus_tag="PKI50_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT04049.1"
                     /translation="MRGKAKEMTPEVTWQEITPGGTIYETGNAEGYKTGDWRSMRPIW
                     IEEKCTQCMLCPPVCPDTAIPVKDSKRLDFDYDHCKGCGVCFKVCPFDAIDFIKEEDK
                     EE"
     gene            10524..11705
                     /gene="porA"
                     /locus_tag="PKI50_09880"
     CDS             10524..11705
                     /gene="porA"
                     /locus_tag="PKI50_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNT04050.1"
                     /translation="MSIRERLSGNEAIAVAMRQINPDVVAAFPITPSTEIPQYFSQFV
                     SNGQVQTEFVAVESEHSAMSACIGAQAAGARTMTATSANGLALMWEMLYIASAMKLPI
                     TLSCVNRALSGPLNIHNDHSDSMGARDSGWIQLYSETNQEAYDNLLQAIRIAEHKDVL
                     LPVMVCQDGFITSHAVENIELLEDDKVKEFVGEYKPDHYLLNHKEPIAVGPLDLPAHC
                     FEHKREQAQSMINAKKVILEAAKEFEKLTGRKYEYFEKYNLDDAEIAIVIINSSAGTA
                     KTVADKLRDNGIKAGLLKIRMFRPFPADEIAEALKNVKALAVMDKAESYSAMGGPVGA
                     EVKSALYGKADGIKVLNYIYGLGGRDVRADDIEEVYNSLMYIVKTGQIGETYRYIGVR
                     G"
     gene            11722..12660
                     /locus_tag="PKI50_09885"
     CDS             11722..12660
                     /locus_tag="PKI50_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT04051.1"
                     /translation="MAYNLKQVMNKPERLTGGHRMCAGCGAPVTVRTVLRALKPEDHA
                     VCCSATGCLEVSTFLYPYTAWKDSFIHSAFENAGATLSGAEAAYTALRRKGRMNESYK
                     FIAFGGDGGTYDIGLQSLSGAMERGHDMVYICYDNEAYMNTGIQRSSGTPRYADTTTS
                     PQGTEMPGKMQYKKDLTGVMAAHRIPYVAQSTLTSNFKDLYEKSEKAIYTKGPAFMNV
                     LSPCPRGWRYDTPDLMEICKLAVETCIWPLYEVEDGKWKLNYKPKKKLPVIEYLKPQG
                     RFKHLFTDENKHLIDNIQEDVDRKWEELLKKCGEDS"
     gene            12837..14846
                     /locus_tag="PKI50_09890"
     CDS             12837..14846
                     /locus_tag="PKI50_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT04052.1"
                     /translation="MRLSFKITVPIVILLIAAIASLSGVSFYFTGKLIESNMSQLSGS
                     KLEEVQNIILQRNAEVSISRMELKMEFLNKAEILANVIKQNPGFIQNNSALFDMTASL
                     EVDEIHITDENGIIRWGTIATSYGLDLNYNERMKPFLPALTDSNFEFVQDPIVRGSDN
                     ALFQYAGVARKDKPGIILIGVSGLKLQSELEKADISGISKDTSFGANGIVIIVDKESD
                     KIISHKDMAIQGGKAAEYDWGRKIRESDSGEFKYISDGTEYFTKYQDTGQYIVCTVIP
                     VSEFTSGMSLFLRNIVMISAAALVLCILIIYILLKINVTNEINKLLKSLKTIGEGDLT
                     KTIDIRSSKEFSKLSEGINVMSNNLREIIEKGLEMTKSLSESGKRLAESADISSKGAS
                     EIATTINELAEGATEQADGATKGALTAKDVLSKAEAISLNIEDTVKNTELTKDTVLAG
                     VEIIKYQNEKMKESVLSAEDLGSSINDLSKRADEIGDITEVITGIANQTNMLALNAAI
                     EAARAGEIGKGFAVVADEVRKLAEGATKSALKISEIIAEIQSGIGNAKIQADKSIRAI
                     EEQQTAVKQTEEAFIKINNVTQEAVNQVGRIAEATENIISGIHKIVDIVESQAAVSQE
                     SAAGTEEITASMQEQTAAIEEVAQIANDLAEVVGELNALISKFKI"
     gene            14993..15139
                     /locus_tag="PKI50_09895"
     CDS             14993..15139
                     /locus_tag="PKI50_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04053.1"
                     /translation="MSIVCEKVIEQFGQDKKLINLIIYNGESIIYNLNDEELNAEELP
                     VLTN"
BASE COUNT         5219 a         2672 c         3695 g         3804 t
ORIGIN      
        1 tgaacagaat aaattttagg cttagaacgc tactgtattt tttaatagta tctataatcc
       61 cctttgcggt gtcatgggct tttatttatg attcttatta caaaaaaatg cagcaggatt
      121 tcagaagctt caaccacatg tacatacaga accaattcag taaaatcgga cagcagatgt
      181 atcaacatga aatggtgtta aaatcagttg ctcaggcata tagttttttg gatcctcaga
      241 ctgccgacct aaatgctttt ctaagagatc agaacagtgt gaatctaatt ttcaggaact
      301 tatccattat tatgcctgat aattcagtct atacaggcaa tccgggctac ttaaagcccg
      361 atatcaatta tacgttactg ttcagctata ttaatgcaag aaaggcccag gaactggtgt
      421 ggcttgaatc ctataaagaa accttaagcg gagttcaatg cgtagggatg tccatacctt
      481 tgctggataa taataacaaa gcttcagggg tattggtcgg aaacatatct ttatggacct
      541 ttgaaaatct gttatccaac gcaaagtatc tgccttatgc agaggtattt cttgtcaatc
      601 cggcaggcta tgtaaaatac cacagcggaa ataagtacag cgaaattgtc aatgtaaccg
      661 ataaagactt ttttcttagc cctgcggcag attccatact taaatcggat gaaggctatg
      721 aagaattcag ttatgcaggc aaaaactggc tttgcagctt ttcttctatc aattccgccg
      781 gatggaaggt tctatccctc atagatgctc aaatgcttca aagcaccttc ggtgttatga
      841 atcaaaatat caacagatct ataatgtctt cgggaatact ttgtattctc ataggaattg
      901 cggcttcatt ttttatgtcc aattcaataa caactcctct tataaagctg agggatgggg
      961 tcaaagcaac tacagaaggc aatttgaata acaggattat tgtaaaaggc gataacgaaa
     1021 taagggaagt agccgatgct tttaatgaga tggcaagtaa cctcaataaa acatatactg
     1081 acctggttaa gaggacagaa gaactttacg ccaacaatga agagcttcat aatgtaaata
     1141 ttgaattgga agcctcttac ggacaattag gggcgacagt cgcacagctt aacgagtcgg
     1201 atgcacagct cagaaggaaa aacagtgaac tgcaaaccct taacaaaata agcaatgcct
     1261 tgaattccac aatggatttg agcaccatgc tcattacagt tgttaaccag gtcactgaca
     1321 taaccgaatc attggcttgc acaataaggc ttataaatga aaaggatcca tacaagctgg
     1381 agcttaaagc tatgaaagga ataaataccg agatgtatga cataggtacc attgatataa
     1441 gggaggacgt aataggcaga gcggtgaaaa ccggatccat atatataata gatcttgata
     1501 atgagaaagt tcctcacgaa tattttatga ggctgaataa agaacatgat gccaaatgta
     1561 ttgtttttac tccgattatg gttaaatcca aggtaattgg cgtcttgagc attaccctga
     1621 agcagattcc cagcgaggaa cttattgagt tattaaattc attgacaaac agtattgcca
     1681 ttgccttaga caatgcaaaa gcttatgagt ctctgaagca gtcataccac aaaactgtgc
     1741 ggagcctggt atcggtggtc gaagcaaagg atcaatatac ggagagtcat tccataagag
     1801 ttgccaagta tgcttccttt attgcttccg aattgaatta cccaaagaat tttattgaag
     1861 atatctgggt ggcggggctg cttcatgata ttgggaagat cggtatcagc gactcaatac
     1921 tgaataagcc cggagccctc actaaagagg aatacgatat tatcaagcag cacccggaaa
     1981 tagcctataa gatagtatcc aaaataggcc tgagagagaa tgtcttaaaa gcaataaagc
     2041 atcaccatga aagatgcgac ggcaaaggtt atcctgatat gataaagagc ggcgaaatac
     2101 cgattatggc ttcgataata agtgttgcag atgcctttga tgcgataacc tccaaccggc
     2161 cttatagaaa atcaaggagt atgaagcagg gtatagatga aatagtcatg cacagaggta
     2221 cacagtttga ctcaactgtg gcagagactc tgaaaaaagt atttctgaca aaacatgaga
     2281 tcctggagag aatatataac gacgaagaaa ttgaattctt ctaatgctca ggaatgagca
     2341 ttttttttat agttaattta ctatgacgct ttattatgct aatattttat tgtacaggaa
     2401 taacacatgg aggataacta tgtatacggc tttatacaga aaatggaggc cccagaggtt
     2461 tgaggatata tgcggccagg aacatataac agtgacattg aaaaatgagc ttgagagcgg
     2521 caggctttcc catgcatatt tgttctgcgg caccagaggc acgggcaaga ccactactgc
     2581 aaagcttttg gccaaggcag tgaactgcaa ggcctctcat gggattgatc cttgcaatga
     2641 atgtgagagt tgtgtcagta taaatgacgg aaactcaata gatgtatatg aaatagatgc
     2701 cgcttcaaac aggggaatag acgacataag gaatttgagg gaagcaataa ggttcacgcc
     2761 taccttgggc gaatataagg tatatataat agatgaagtt catatgctaa caaatgaggc
     2821 gttcaatgca cttctcaaaa ccctggagga accgccggaa tatgtattgt tcatattggc
     2881 aacaactgaa cctcataaac tgccggctac aattctatcc cgatgccaga ggtttgattt
     2941 taaaagaata agcagcgaaa aaatagttga gcggctgaag tttgtctgca gagaagaggg
     3001 cttggaagcg gaagaggatg cagtaaagct tattgcaaga aacagcgacg gggcaatgag
     3061 agatgcattg agtatccttg accaatgtgc ggtttacggc agcaaaaaaa taacccacga
     3121 tgatgtactt gaggtactgg gcattgtgaa caaccaatat ttgttcagaa tagcggaagc
     3181 agtacttgaa gaggacgccg ggacggcaat aaaggtgatt gatgaggtag cggcaggagg
     3241 gaaggatata agccagttta tcagagattt gctggtacat tacaggaatc ttctgatggc
     3301 taaagttgtg acaaactgtg aagatgtgat agatatgtca aaggagggaa ttgagcagct
     3361 taagaaacaa tccggcagtt atgataaaaa caatatcgta agatgtatca actgcctttc
     3421 cgagttggaa aatgaagcaa agtggtcggt caatccaagg atcttgctgg aagttgcact
     3481 ggtaaaaatg tgccgggtcg gagaggaaaa aagcttggag ggtatactca caaggttgtc
     3541 aaaactggag gaacgtattt cgaaaggaag ttttgacctg aaggccgcag ataagccaaa
     3601 ggcagcaggt caggaagaga aaagggaagc ggcagtaaaa aaagaaaaag cagccgcaga
     3661 atcaaaggaa accgcagtat cgaatccgga cagcgggagc ataatcgaaa aatggcccgg
     3721 ttttattcag gaaatgaaag tccgcgggaa aatcaggctc aggacttatc tggcaatggc
     3781 aaagccggtt aaagcggagt caggcataat ggtattatcc tttgaaaaag aaggggtgtt
     3841 ttccaaagaa gcccttgaac tgcctgcaaa cagggcagat gtggaggaag ccgcatcaaa
     3901 atacttcggg gcacctctta agataaagtg tattatttcc ggtgaggaag aagatgaaca
     3961 ggacagcatt gtaaaagctg ctattgaatt tgcaggcatg gataaagttg aagttgttga
     4021 agaagaggag gaataagaaa tgggtaaagg cggattcccc ggaatgggag ggaatatgaa
     4081 taatatgatg aagcaggttc agaaaatgca aagagaaatg gcaaagcttc aggaggagat
     4141 agaggggaga agcgttgaag ccagcgccgg cggaggggcc gtaaccgtag tagccaacgg
     4201 aaagaaagag atactatcaa taacaataaa acccgaagtt atagaccctg atgatgcaga
     4261 aatgctccag gatcttatca ctgcggctgt taacgaagct ataagacaag cggatgaaat
     4321 gataagcaga gaaatgagca agttaaccgg agggctcaat ttacccggag gtttattcta
     4381 atgagttttt atgcagctcc tatagccaag cttatagatg agctttcgaa gcttcccgga
     4441 gtaggcaaca agacagcaca gcggctggca ttccatatac tcaacatgtc ctccgaagat
     4501 gtagagaagc tttcttcctc tatagtaagt gcaaagagaa atatcagata ttgcaaggtg
     4561 tgctgcaata tcacggattc tgatctatgc gatatctgtg ctaatgcaaa aagagacagc
     4621 agtaccatat gtgttgtgga ggatcccaag gacgttgtcg caatggagaa gacaagagaa
     4681 ttcaaaggct tgtatcatgt attgaacgga gcgatatcgc ccatggaagg catagggccg
     4741 gaggagatca gaatcaagga actccttcag agggtggctg acaatgaaat aaaggagata
     4801 atactggcga ctaatccgaa tatagaagga gaagcgacag ctatgtatat ttccaggctg
     4861 ttgaagccta tcgggatcaa ggtaaccaga atagcccacg gtgtacctgt aggaggggac
     4921 cttgaatatg cggatgaagt gacattgatg aaggcccttg aaggcagaag ggaaatatag
     4981 gctaaattta tcttaaaata gagtacacct cagaatatta ttgctaattc ttggataaat
     5041 tagtaataat aacggaggtg ttttatttat gtccacgaat ctggcagata gggagcctaa
     5101 gaaaaataaa cagatagagg atattataga ctgtttgctg aatagcatga tgggcaccag
     5161 ggaaagggac gaaaatgcgg agctgataaa tacgatactt atagcaaagc aggaaatgca
     5221 tgacgcacaa aactattttg acaatgtaac cgcccctgag cttgtagatc atgcaatatt
     5281 cagaatggaa gctgccaaat cacaatatgt ttatctgctg aagcttgcaa aggacaaggg
     5341 gctcagcgtg acgatatagc cggcctgtga taaaatagtt gtacaaaact tgtaataaaa
     5401 tacgaaaaga gtaatataaa taaacatacg atctttataa cttaccgggg gggattcaag
     5461 ttgccgcaga tagatgtatt ctcaatactg gcagtatttt ttgccatagg tgctgtctat
     5521 gttataggtg tattgctggt cttgcctatt aaaataattg tcaaactggt aatgaacggt
     5581 ataatagggg cggttgcatt atttgcaatc aatattttca gcggactcac aggcctctcc
     5641 atagggataa acccaattac agcactggta gcaggatttt tgggaatccc cgggataata
     5701 cttttattgt tcctgcaata tattttgtag caggtttgaa aaaacttgtc agatgaataa
     5761 taattgatag atgttcataa ttataaaatt gaccaaaaaa taactaactg cctttaccca
     5821 ttgagagaca tggatgaggg tggttattat tttttaaggg ttcaatgctt ttaatttgac
     5881 aatgaaatga atgtttaata tactaaaagt ggcgttcttt gataaattaa ctacaaatga
     5941 ccatttatta ttatattagg gggtttagca atggctaaaa agatgaaaac aatggatggt
     6001 aatgccgcag ctgcgcatgt cgcttatgcg ttcacggaag ttgctgcaat ttacccgatt
     6061 acaccgtcat ccacaatggc tgaaaatgtt gatgagtggt ccgcccatgg actgaagaac
     6121 atttttggcc aacaggtaaa ggttgttgag ctgcagtcag aaggaggagc tgcaggagca
     6181 gttcacggtt cccttgcagc gggtgcatta actacaacct ttacagcatc acagggattg
     6241 ctccttatga taccaaatat gtacaagatt gccggagaat tgcttccggg cgtattccac
     6301 gtatctgccc gtgcattggc aagccacgcg ctctcaatat ttggagacca tcaggacgta
     6361 atggcaacaa gacagacagg atttgcttta cttgcttccg gttcagtaca ggaaataatg
     6421 gatctgggcg gtattgctca cttggctgca atcaaatcaa gagttccgtt tgtacacttc
     6481 tttgacggtt tcaggacctc cagcgaggtt cagaaggtag aagtaatgga ttatgcagac
     6541 ctggaaaaac ttcttgacaa gaaagcattg gatgaattca ggaaaaatgc tttgaatccg
     6601 gagcacccgg taacaagagg tactgctcag aatcctgata tatacttcca ggcaagagaa
     6661 gcttgcaata agtattatga cgcagttccg gacatagtag cagactatat gaaggaaata
     6721 agcaagctga caggcagaga atacaaaccc tttaactatt acggggcgcc tgacgctgaa
     6781 aacatcatag tagcaatggg ttctgtgacg gatgctgttg aggaaacggt agattattta
     6841 atggcaaaag gtgaaaaggt aggagttgta aaggtacacc tctacaggcc tttctcagaa
     6901 aagtatttct tcgatgtact tccaaaaaca gtgaagaaaa ttgctgtact tgacaggaca
     6961 aaagaagtcg gatcaacagg agaaccatta tatcttgacg ttcgttcaat gttctataac
     7021 aagaaaaatg ctccattaat agttggagga agattcgggc ttggttccaa agatactaca
     7081 ccgtcacaga ttaaagctgt ttttgataat ttgaagacta acgagccaaa agacggattc
     7141 acgataggta tagtagatga tgttacaaat ctttcccttg aagtaaagga aaatatcaat
     7201 gcagctcccg aaggaacaat aagatgcaag ttctggggtt tgggttccga cggtacggtt
     7261 ggagcaaata agagtgcaat aaagattata ggcgataaga ccgatcttta tgcccaggga
     7321 tattttgctt atgactccaa gaagtccggg ggtgtaacca tttcccacct cagattcggc
     7381 aagaagccta taaaatcccc atatcttatt gatgaggctg atttcatagc atgccacaac
     7441 caggcatatg ttcatcagta tgatgtaata aagggactta aagacggcgg cactttcgta
     7501 ttgaactgca aatggaatgc agacgagctt gaagagaatc tgcctgcaaa tatgaaaaag
     7561 tatatcgcag atcataaaat caagttctac ataatcaatg cgacagatat tgccgtaaaa
     7621 ataggccttg gaaacagaat caacatgata atgcaatctg cattcttcaa actggccaat
     7681 gttattccga tagaagatgc tgtaaagtac cttaaggagt ctcttgcaga aacttacggc
     7741 aagaaaggcc agaagattat tgaaatgaac tacaaagctg ttgatgcggg aaccgatgcg
     7801 cttgtaaaag ttacagttcc ggaaagctgg agcagtgcaa aagaagaagc ggcagcggca
     7861 gtggatgttc ctgattttat aaagaatgta cttattccta tgaacaggca ggaaggagat
     7921 acacttccgg taagtaaatt tgttgagtat gcagacggac atttgccaat gggaactgct
     7981 gcatacgaaa agagaggaat agcagtattt gtacctgagt ggcagaagga aaaatgcata
     8041 caatgtaatc agtgctcctt tgtatgtcct catgcggcaa taagacctgt gcttgtagct
     8101 gaagatgaat taaagaaagc acctgcagga tttgaaacaa taaaagcttc aggaaaaggt
     8161 tttgaaggag tatcttacag gatgcaggta agcccattgg attgcacagg ctgcggaaat
     8221 tgtgcagata tatgccctgc aaaagaaaaa gcacttgtaa tgaagcccct tgatactcag
     8281 acagaaaaag gtattgataa ttggaacttt gcaatgactg tacctgttaa ggacaactta
     8341 atgacaaagt caactataaa aggcagccag tttgcacagc catatctcca attctcagga
     8401 gcatgcgcag gctgcggcga aactccatat atcaaggttg tgactcagct ctttggtgaa
     8461 agaatgatga tagcgaatgc aacaggatgt tcatcaatct ggggcgcttc aacaccttca
     8521 actccatact gcaaggacgc aaatggaaga ggcccgtcct ggggtaattc cctctttgaa
     8581 gacaatgcag aatatggatt tggtatggcg gtagctgtaa atcagataag agcgaagctt
     8641 gccgaaataa tgacagaagc attggctttg aatatgccta atgaatataa ggaagcatat
     8701 aaagcatggc tggatggcaa ggatgatgca gatgcaagca aagcagcagc agaaaagata
     8761 cttccccttc tttacaatat gagtgcatta agcggaagag caaaagaaat agcagctgag
     8821 atagctgaca agaaagacca gcttgtaaag aaatccatct ggatactcgg tggtgacgga
     8881 tgggcttatg atataggcta cggcggactt gaccatgtac tggcatccgg agaaaacgta
     8941 aacgtaatgg tatttgatac ggaagtatat tccaacaccg gaggacagtc ctccaaggcg
     9001 acaccggtcg gagcagttgc caagtttaca gcatccggta agaagacaaa gaagaaggac
     9061 ctgggtatga tagcagctac ctacggatat gtatatgtag ctcaggtagg tatgggagca
     9121 gacaagaatc atttcctgaa ggttattcag gaagcggaga agtatgacgg accatctctc
     9181 ataatctgtt atgcaccatg tatcaaccac ggacttaagg aaggcatggg gagaacccag
     9241 gccaacactg ctcaggctgt ggaagcagga tactggcacc tgtggagata taaccctgaa
     9301 ctcaagaagg aaggcaagaa tcctttcata cttgactcaa aggagccaaa ggcaagcttc
     9361 agagatttcc tgatgaatca ggtaagatat tcatccctgg tgggtgcttt ccctgaagaa
     9421 gcagaaaagc tctttgcaaa agcagagaag gaagcaaagg aaaaatacga aaactacaag
     9481 aagatggcag aagcttaaaa ataaaaatca gaaaaaagtc gattgagaaa tcaatcgact
     9541 ttttattttc ataaaaaaat caaaatagca cagaatacca atgtattaga aagaaaagat
     9601 atgaagggag gaaaggtttt gggcaagtta atagaaatac gctggcatgg gcgcggtggc
     9661 caaggagcga aaacagccgc acttctcctg gcagatgccg cttttaacac aggaaagtat
     9721 attcagggat ttcccgagta tggccctgag aggatgggag caccgataac agcctacaac
     9781 agaataagcg acgaaaggat aaaaattcat tccaacattt atgagccgga ttatgtagta
     9841 gtggttgatg agtctttgat ttcggcaatt gatgtgactt ccgggcttaa agagacgggt
     9901 gcaattatca ttaatactga taagaaaccc gatgaggtga agaaacatct aaaaggatac
     9961 aagggcaagg tttgcaccat agacgccagg aagatatctg aagaaaccat aggcaggaat
    10021 tttcccaata cacctatgct tggcgcggtg gtggtagtcg gtaatattat ggagcctcag
    10081 aagtttctgg aggatatgaa ggagtccttt gctcataagt ttaagacaaa gcccgagata
    10141 attgaaggga acatgaaggc cattgaacgg gctatgcagg aggtgcaaga gctatgagag
    10201 ggaaagcaaa agagatgact cccgaagtga cttggcagga gataaccccg ggaggcacta
    10261 tatatgagac gggtaatgca gaaggctata agacagggga ctggagatcc atgaggccaa
    10321 tctggattga agaaaaatgc acccaatgca tgctgtgtcc gccggtctgt ccggacactg
    10381 caattcctgt aaaggacagc aagaggctcg attttgatta tgatcactgc aagggctgcg
    10441 gagtatgctt caaggtatgt cctttcgatg caatagattt tatcaaagaa gaagataaag
    10501 aagaatagga ggtgcagttg cacatgtcaa taagggaaag gttatcagga aatgaagcaa
    10561 tagcagtggc aatgaggcag ataaatccgg atgttgttgc tgcattccca ataactccgt
    10621 caacggagat accccagtat ttctcacaat ttgtatctaa cgggcaggtg cagacagagt
    10681 ttgttgcggt agaatcagaa cacagtgcaa tgtcagcctg cattggagca caagcagcag
    10741 gagcaagaac aatgactgcc acttccgcaa acggtttggc tttgatgtgg gagatgttat
    10801 atattgcttc agcaatgaag ctgccaataa ctttatcctg tgtcaacaga gcactgtcag
    10861 ggcccctgaa catacataac gaccacagtg attcaatggg agcaagggat tccggctgga
    10921 tacagctgta cagcgagacc aaccaggagg catatgacaa tctgcttcag gctataagga
    10981 ttgctgagca caaagatgtt ctgcttccgg tcatggtatg ccaggacggc tttattacca
    11041 gccatgcagt agagaatata gagcttcttg aggatgacaa ggttaaagaa tttgtaggag
    11101 aatacaaacc ggatcattat ctattgaacc acaaggaacc aatagccgta ggaccccttg
    11161 accttccggc tcattgcttc gagcataaaa gagagcaggc tcagagcatg atcaatgcaa
    11221 aaaaagtaat actggaggca gcaaaagaat ttgaaaaact taccggaagg aaatacgagt
    11281 atttcgagaa atacaatctt gatgatgcag aaattgctat tgtaataatc aattcttctg
    11341 ccggaacagc aaaaacagtt gcagacaagt tgagagataa tggtataaag gcaggacttc
    11401 tgaaaataag gatgttcagg cccttcccgg ctgatgaaat cgcggaagcc ttgaaaaatg
    11461 taaaggctct cgcagtaatg gataaagccg aaagctattc tgcaatggga ggccctgtcg
    11521 gtgctgaagt aaaaagcgca ctctacggga aagcggatgg aataaaggtt ttgaactata
    11581 tttatggttt aggcggcagg gatgtaaggg ctgatgatat cgaggaagta tataactccc
    11641 ttatgtatat agtaaaaaca ggtcaaatag gagaaactta cagatatata ggtgtaagag
    11701 gataggaggc gggatatttt catggcatat aatcttaagc aagtaatgaa taagccggaa
    11761 agattaaccg gagggcacag gatgtgtgca ggctgcggag cgcctgttac ggtgagaacg
    11821 gtattaagag cattgaagcc tgaagatcat gccgtatgct gttcggctac aggatgtctt
    11881 gaggtatcta cattcctgta tccttatacg gcctggaaag attcatttat tcattcagca
    11941 tttgaaaacg cgggagctac actgagcggc gcggaagctg catatactgc tctaaggaga
    12001 aaaggcagaa tgaacgaatc ttataagttc attgcctttg gaggggacgg agggacttat
    12061 gatattgggc ttcagtcact ttccggagcg atggagagag gtcatgacat ggtgtatata
    12121 tgctatgaca atgaggcata tatgaacacc ggaattcaaa ggtcatcagg aacaccaagg
    12181 tatgcagata cgactacaag cccgcagggt accgaaatgc cggggaaaat gcagtacaag
    12241 aaggatctga caggagtaat ggctgcccat agaataccct atgtcgctca atcgacatta
    12301 acttcaaact ttaaggatct ttatgaaaaa tcagagaaag ccatatatac taagggcccg
    12361 gcatttatga atgtactttc tccctgcccc aggggctgga ggtacgacac ccctgatctt
    12421 atggaaatat gcaagctggc agttgaaaca tgtatatggc cgttgtatga agttgaagat
    12481 gggaaatgga agctcaacta caaacccaaa aaaaagcttc cggttattga atatttgaag
    12541 ccccagggaa gatttaaaca cttatttaca gatgaaaata aacacctcat tgataatatt
    12601 caagaagatg tcgacagaaa atgggaggaa ttattgaaga agtgcggaga agattcctga
    12661 aaaacgtgca aaagcacgtt tttttatatt gggaataaca gtatatataa atgactttct
    12721 gtaaatattt gtcgatataa atgggaaaag atgctataat agtaatcaaa tgatatgaga
    12781 aaatgtaata actgtgtaat agctattatt aatatagtaa agcgggagga tatgatatga
    12841 gactgagctt taaaatcact gttcccattg ttattctact gattgcagca attgcatcat
    12901 tatccggagt atcattctat tttaccggaa agttaatcga gagtaatatg tcgcaattgt
    12961 cagggagtaa gttggaagag gttcagaata taattcttca acggaacgca gaggtatcta
    13021 tcagtaggat ggagcttaaa atggaatttt taaataaagc tgagattctg gccaatgtaa
    13081 ttaaacaaaa tcccggattt attcagaaca attctgcttt gtttgacatg acagcttcct
    13141 tggaggttga cgaaatacat ataactgatg aaaacggaat aataagatgg ggtacgattg
    13201 caacctctta tggtttggat ttgaattata atgaaagaat gaaacctttt ctaccggcac
    13261 tgaccgattc caactttgag tttgttcagg atccaatagt aagaggttca gataatgctc
    13321 tttttcagta tgccggcgta gccagaaagg ataaaccggg gataatactg attggagtat
    13381 caggcctgaa gcttcagagt gaattggaaa aggcagatat aagcggcatt tcaaaggata
    13441 caagctttgg tgcaaacggc attgtcatta ttgtggataa agaatcggat aaaataatca
    13501 gtcataagga tatggcaata cagggcggta aagcagctga atatgattgg ggcaggaaaa
    13561 taagggaaag tgattccggt gaattcaagt atatttcgga tgggacagag tattttacga
    13621 aatatcagga taccggacaa tatatagtat gcacggtaat ccctgtatcg gaatttacat
    13681 ccgggatgag cctgttcttg agaaatattg taatgatatc tgcagcagct cttgtgcttt
    13741 gcatacttat aatatatata ctccttaaaa taaatgttac caatgaaatt aacaagttat
    13801 tgaagtcatt aaaaactatc ggcgaaggag atcttacaaa aacaattgat ataaggtcca
    13861 gcaaagagtt ttcaaagctt agcgaaggca ttaacgtgat gagtaacaac ctgagagaaa
    13921 taatagaaaa gggacttgag atgacaaaaa gccttagcga gtcaggtaaa aggctggctg
    13981 agtcagcgga tatatcaagc aaaggtgctt cggaaatagc aacaacaatt aatgagcttg
    14041 cagaaggagc cactgagcag gcagacggtg ctacaaaagg agcgttgact gcaaaggatg
    14101 ttctgagtaa agctgaagct atttccctca atatagagga cacagtcaaa aatactgaac
    14161 ttaccaagga taccgttctg gcaggtgttg aaataatcaa gtaccagaat gaaaaaatga
    14221 aggaaagtgt attaagtgca gaagaccttg gcagctccat caatgatttg tcaaaacgag
    14281 ctgatgaaat cggtgatata acggaagtca taaccggaat agcaaatcag accaatatgc
    14341 ttgctttaaa tgcagcaatt gaggcggcaa gagcaggtga gatcggaaaa ggatttgctg
    14401 tggttgctga tgaggtaagg aagcttgctg agggtgccac gaaatcagca ctcaagatat
    14461 cggaaataat tgcagagata cagtccggca tagggaatgc aaagatacag gcagataaat
    14521 ccataagagc aattgaagag cagcagacag cagtaaaaca gactgaagag gccttcatca
    14581 agataaacaa tgtgactcag gaagctgtaa atcaggttgg cagaattgca gaagctaccg
    14641 agaatattat aagcggtata cataaaatag ttgatattgt agaatctcaa gcagcagttt
    14701 cccaggagag cgctgccgga accgaagaaa taaccgcatc tatgcaggaa cagacagcag
    14761 ccatagaaga agtagcacaa atagccaacg accttgcaga ggttgtaggc gagctaaatg
    14821 ccttgataag caaatttaag atataacgag gcaaaaagat gtccaacagg cggcgagcat
    14881 atctcccgcc tcttttttat ttgcatttaa attctgaatt aaatcataca taatacaaaa
    14941 aataatacaa agaagttttt ttctatccat aaacaaaatt agagatgaag atatgagtat
    15001 tgtttgtgaa aaggtgatag agcagtttgg tcaggataaa aaacttatta accttattat
    15061 atataatgga gaaagtataa tatataactt gaatgatgaa gaattgaacg cagaagaact
    15121 tcctgtgctc accaattgac atttatcata tatgtccctt ccggacagcg ctgctgtctg
    15181 tcagcaatga agcaagcttc attaaatgga caaaatcaag gtatgttata tgataaacgc
    15241 atggaatgaa ttacttattg acgtttatca tatatgtccc ttccggacag cgatgctgtc
    15301 tgtcagcaat gaagcaagct tcattaaatg gacaaaatca aggtatgtta tatgataaac
    15361 gcatggaatg aattacttat tgacgtttat
//
LOCUS       DAOQUR010000055        14944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4526_length_14944_cov_9.384243, whole genome shotgun sequence.
ACCESSION   DAOQUR010000055 DAOQUR010000000
VERSION     DAOQUR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14944
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_14944_cov_9.384243"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PKI50_09900"
     CDS             complement(<1..1344)
                     /locus_tag="PKI50_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNT04054.1"
                     /translation="MTEKRENWGSRFGFLMAAAGSAVGLGNIWRFPYLTGMNGGAAFI
                     FIYLGCVALIGLSIMTAEFAVGRRSGLAAVGAYKKVSDKWSFAGALGVLSGFFIMGFY
                     PVVGGWAMAYIAKSFTGLLAAPEAIGDAFGAFISAPVEPLVWMVIYLVINVLIVAKGI
                     QSGIEAAGKVMMPMLYVLLAAIIFRTASLPGASEGFKFLFTPDFSKVTGQTFLAALGQ
                     AFFSLSLGMGCMITYGSYLNKEEKLPQNALIVTTMDTSVALLAAIAIFPALASFGVEP
                     TAGPGLVFVVVPQIFAQMGGIGPIFSALFFLLLFIAALTSSVSLLEVVVAYLIDQHKM
                     ARKKAVYLTSAIMAVMCMLSSLSMGVMSGFTIFGVGFFDFFDILTDKIFLAVGGGLLA
                     ICVGFFMKKEDLKDELTNGGTIEFGLFELWYNVTKYVIPVIIAVIAFSGIMAIQQK"
     gene            complement(1534..2109)
                     /gene="pyrE"
                     /locus_tag="PKI50_09905"
     CDS             complement(1534..2109)
                     /gene="pyrE"
                     /locus_tag="PKI50_09905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNT04055.1"
                     /translation="MLTKKRVNGILEKTGVLLTGHFQLTSGKHSNKYLQCAKVFQHPD
                     ISAEISKDLAEKYDGYDIDIVIGPAVGGIILAYEVARQLEVRALFAERENGIMTLRRG
                     FEIQRGSKVLVVEDVITTGGSVKEVIDVVRSAGGEVVGVAAVVDRSGGKALFDVPFKS
                     AIKIDIEVFEPGDCPMCKSGSKAVKPGSRNI"
     gene            complement(2088..3029)
                     /locus_tag="PKI50_09910"
     CDS             complement(2088..3029)
                     /locus_tag="PKI50_09910"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNT04056.1"
                     /translation="MTRMSVNIGGIKLKNPVLTSSGTFGFGREYSGFYDLSKLGGIVV
                     KGLTLEPREGNKPPRIAETPAGMLNSVGLQNPGVEYFIEKELPFLRGYDVAVIANIAG
                     NTVEEYCMMARRLGSLVDGIELNISCPNVKEGGAAFGINSESVYNVTRRVKEQCKVPL
                     VVKLSPNVTDIKEIAEAAKEGGADALSLINTLLGMAIDINSRRPVLANNVGGLSGPAV
                     KPVAVRMVWQAAQAVDIPIIGMGGISCWEDAIEFMLAGADAVSVGTANFINPYAPLEI
                     LEGIEKYLIKNQFSNINELKGNLKIYGGDNKCLQKKE"
     gene            complement(3022..3777)
                     /locus_tag="PKI50_09915"
     CDS             complement(3022..3777)
                     /locus_tag="PKI50_09915"
                     /inference="COORDINATES: protein motif:HMM:NF000798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNT04057.1"
                     /translation="MQCICKIVENRELCKDVFEMKIECPEMVREAQPGQFLHLKVNKS
                     IDPLLRRPISISRIYRDTGVISIVYQCVGKGTKEMTALKKGEYIDALGPLGSGFTLSD
                     EGKCAVVGGGIGIAPLLELACSLKDCDAYLGFRCNTYKLDEFSSACKELYIATEDGST
                     GSKGYVTDIIKDITKYNVIYACGPKGMLKRVKELCEDTGVKCFVSVEERMGCGIGACL
                     VCACRIKEGDTWHYKKACTDGPVFAAEEVDFDD"
     gene            complement(3792..4730)
                     /gene="pyrF"
                     /locus_tag="PKI50_09920"
     CDS             complement(3792..4730)
                     /gene="pyrF"
                     /locus_tag="PKI50_09920"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNT04058.1"
                     /translation="MFTDRLIEKVIKCRNHTVVGLDPRLQYLPDYLIDEAVEAVAEGS
                     RASVIAEAFFRFNKEIIDNVYDIVPAVKPQIAFYEQYGTAGFECYLKTCRYAKSKGLL
                     VIGDVKRGDIGSTAEAYSDFYLGNNKASAETDCITINPYLGTDSMIPFVNNCEKNGKG
                     IFVLVKTSNKSSKDIQDLESGGKKVYEHVAELVVRLGEGLTGKYGYSSVGAVVGATFR
                     SEGQRLRELMKGVYFLVPGYGAQGGTAEDAAVCFNDDGLGAVINSSRGIIAAYKSEAY
                     RERFSEKEFGLAAREAAIAMRHDINNELAGTGKIAW"
     gene            complement(4742..6028)
                     /locus_tag="PKI50_09925"
     CDS             complement(4742..6028)
                     /locus_tag="PKI50_09925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNT04059.1"
                     /translation="MRLLIKQCSIIDPGNTYEGINDIYIEDGLIKLIEPSIGLAADKV
                     IDAEGLLLLPGFIDMHAHLREPGFEYKETVYSGTRAAARGGYTSICCMPNTRPVIDDE
                     ASLSMLQKIIKKDAAVKVYPIAAISKGQKSTELVEMNKLSEMGAVAFSDDGRPVATAA
                     FMMKALLSAKEKGFMIIDHCEELSLAEGGAINKGRKSEQMGIRGIHPLSEELNIMRDI
                     MIAGNTDSHIHIAHVSTAGSAETIREAKKRGVKVTCEVTPHHIGLTEDIITPGFTDCK
                     VNPPLRTAGDVEALKEALKDGTIDVIATDHAPHHKDEKSEDFYSSAFGISGLETAFSV
                     CYTELVEPGILTLKELSERMSKNPAEILKLDTGIIRTGIAADLVLVDTAREITVNRND
                     FLSKGKNTPIHGKTYKGDIVYTIVDGNVAYENKNRL"
     gene            complement(6078..6617)
                     /gene="pyrR"
                     /locus_tag="PKI50_09930"
     CDS             complement(6078..6617)
                     /gene="pyrR"
                     /locus_tag="PKI50_09930"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNT04060.1"
                     /translation="MRIKTEIMDEKALDRALTRISHEIIERNKGVEGLVLLGIKTRGV
                     SLAKRLCEKIRNIEGNVLPVGTLDITRHRDDIKDMKDIPRENLTDISFDINGMKVVLV
                     DDVLYTGRTVRAAMDAIVEIGRPLSIQLAVLVDRGHRELPIRADYVGKNVPTSRSEMI
                     IVNVGEVDGKDAVLLGEQE"
     gene            complement(6758..7696)
                     /locus_tag="PKI50_09935"
     CDS             complement(6758..7696)
                     /locus_tag="PKI50_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT04061.1"
                     /translation="MEVKEYIAGPEAEGERLDCFLSGKVEGYSRTYMQKLIEEGHCKV
                     NGKNAKPRFKLREGDRVEAVIPDPVPLEVEPEKIDLDIIYEDNDIVVINKPQGMVVHP
                     SHGNYSGTVVNALMGHCKNLSDYNSLTGINGVMRPGIVHRIDKDTSGIIVVAKNNEAH
                     LSLSGQLKEHTITRKYTALLEGRLKKECGTADTLIGRNPKNRKQMAVVRENGKRAVTH
                     YRVLESFENHTLIEAVLETGRTHQIRVHMAYLGHPVVGDTVYGYKKQRFDTKGQLLHA
                     RVLGFIHPAKKEYVEFEAPIPEYFEKVLRLLRNGRI"
     gene            complement(7787..8230)
                     /gene="lspA"
                     /locus_tag="PKI50_09940"
     CDS             complement(7787..8230)
                     /gene="lspA"
                     /locus_tag="PKI50_09940"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNT04062.1"
                     /translation="MYPFLIAAAIIVSDQVIKNILANALASGPITVIKNFFYLVYVEN
                     YGIAFGMFKNKVLFFIVTNCIISAAVAFMIYKYRNKSVPVTICLSLILGGAIGNVIDR
                     IRFGYVIDYLSFTIFPPVFNLADSAIVVGAILLSLLLIFGKDIKI"
     gene            complement(8289..8897)
                     /locus_tag="PKI50_09945"
     CDS             complement(8289..8897)
                     /locus_tag="PKI50_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNT04063.1"
                     /translation="MDKNDFKHFEELLKEEKKKRENSLEDERTGLGISVKDLTGELSS
                     YDNHPGDMGNETFEAEKNFSFRTRDKFLLSEIDAALNRIADGTYGSCEICHNEIGKDR
                     LELRPYSRLCINCEKKYGSNVQNEEKGRPIEEQVLYPPFGRTFTDNSVEDKVHFDGED
                     AWESVNEYNVRMSDNEFGEDESAGYVEDVEQVSNEQYRKQLE"
     gene            complement(8919..9209)
                     /locus_tag="PKI50_09950"
     CDS             complement(8919..9209)
                     /locus_tag="PKI50_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HNT04064.1"
                     /translation="MDIDNTEVKELESRIEKIALLLEKAKIGEYVNIMTKPKTLLLNN
                     FIGGVARGFGTAVGFTILGAVVIYILRQSVLLNLPFIGGFIAEIVKIVQDHL"
     gene            complement(9309..9758)
                     /locus_tag="PKI50_09955"
     CDS             complement(9309..9758)
                     /locus_tag="PKI50_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNT04065.1"
                     /translation="MITPMDIRNKEFKKAFKGYKEDEVDEFLDKVVADYERIYRENGE
                     LKDRIAIDNDRIESYNSMEKSLQSTLLIAQTTAEDIVANARKKAEMIIKEAEEQGRKI
                     IEEANSSVIKVNKDFEELKKEVQVFKTRFKTLLESELEALNATLKDI"
     gene            complement(9864..10145)
                     /locus_tag="PKI50_09960"
     CDS             complement(9864..10145)
                     /locus_tag="PKI50_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNT04066.1"
                     /translation="MKFVLAATLSYFIDFLNFMILIRVLLSWFSFNPDSKIIVLIFQL
                     TDPILEPFRKLTAKLGLNTGMIDFSPIISLLFLYYIAKPLLLTLIYTFL"
     gene            complement(10167..10625)
                     /gene="sepF"
                     /locus_tag="PKI50_09965"
     CDS             complement(10167..10625)
                     /gene="sepF"
                     /locus_tag="PKI50_09965"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNT04067.1"
                     /translation="MESNILNKVLYFMGIGEAAEEEHEEAEVSKPQIEAVGTASHRRS
                     NVVNIHTASAPMKVVLVEPSSYDEVQSICDDLRSKKPVIINFEEMDKEVAKRMVDFIS
                     GAVYVLDGTIQKISNGIVLVAPSNVDVLGNIKSSIGDENFDLEGIFSWLK"
     gene            complement(10641..11339)
                     /locus_tag="PKI50_09970"
     CDS             complement(10641..11339)
                     /locus_tag="PKI50_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNT04068.1"
                     /translation="MQEIKNNIIAISRDINDICIKKGKAPDNITIIAVTKTIDPSRVN
                     YAIKCGIRNIGENKVQEIMAKYENIEENVNWHLIGHLQTNKVKYIIDKVALIHSVDSI
                     GLAEEINKRAEKAGLVKDVLVQVNVAQEETKFGIEYDETDSFAERLSGLSSIRVKGLM
                     TIAPYYDDAELARPVFRRLKEKFDMLAAASIPNVDMKYLSMGMTNDYKVAIEEGSNMV
                     RIGTGIFGARNYDL"
     gene            complement(11353..12621)
                     /locus_tag="PKI50_09975"
     CDS             complement(11353..12621)
                     /locus_tag="PKI50_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNT04069.1"
                     /translation="MRAKKNKISSVYIFPVLFFVICLSYFIRFMFFNIETEIVKYGSV
                     ENSIRVKALIIRNESTTILPSGVEIKYNVNEGERVSLGKKILEIVKNDQADENISIKI
                     KQLDDRIQEIKQSDINNNFFSKDKEKIENRIKERVSDLKSIARSGDLKKLDDVKNDLA
                     ANLYKKSLIYGEGSFFGKNLEQLQKEKSVLEGIYNNSIDVIYAQASGVVSKSLDGYEQ
                     ILNIGNIKDFKLSNIREIMSSLTADSQKTSQSTTAGIKIVDNFEWYTCSLISFDQTKD
                     IKAGKKVRLRFSELGNAEVNGEVHEVSQPEGDICMVIIRISDHVNDFYKKRIAEVDII
                     KDYNEGFEVSANAIVVKDNTRGLYVLKKGIVKFIPVAVMNEDKERCLVRNLNKEEWNS
                     KSEYEPLKIFDEVITTTERVKENQVFTDKI"
     gene            12894..13151
                     /locus_tag="PKI50_09980"
     CDS             12894..13151
                     /locus_tag="PKI50_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNT04070.1"
                     /translation="MPNIKPVSDLRNYNEVLRDVAVGEPVFLTKNGRGRYALVDILDY
                     EKAQATIKLMSELAKGQKSGEEKGWLSHEAAKAHFKEKNNE"
     assembly_gap    13173..13272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13352..13428
                     /locus_tag="PKI50_09985"
     tRNA            13352..13428
                     /locus_tag="PKI50_09985"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13386..13388,aa:Pro,seq:ggg)
     gene            13590..14045
                     /locus_tag="PKI50_09990"
     CDS             13590..14045
                     /locus_tag="PKI50_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04071.1"
                     /translation="MSKSFRKPVFSSRFNRLPDSTVEKCGECCLVHEKFSLNLIKKAT
                     NKPSTNTIFKASEFQDCSPVQIGIAQNSSRFPFGSILVRNFSAVPVSFTVFGDTEEET
                     DMTVQSNSEVAVTLNAISAVEIFYPTDPIRLLFIFDIFHPGDPITPPNP"
     gene            complement(14112..14450)
                     /locus_tag="PKI50_09995"
     CDS             complement(14112..14450)
                     /locus_tag="PKI50_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNT04072.1"
                     /translation="MSNQLTAVAALINDKVKFSGVSRDKKEIIIDYVKPIGDGEGYTS
                     LELFLISLATCSGTSVSSLLRKMNKDVYGLNITTYGERREEHPTYFKKYSYILNLCQR
                     MLRFLIWKNP"
     gene            complement(14642..>14944)
                     /locus_tag="PKI50_10000"
     CDS             complement(14642..>14944)
                     /locus_tag="PKI50_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT04073.1"
                     /translation="GGIHFDPNFAQAVFTQWEDDAFGVSHIIANQWLVAGEYEKEHGL
                     AFTSAAVDKIAGGVEAVVLHDKMKGCYKDLVRAVGEKYQIPVLRHQRLRQPEELQL"
BASE COUNT         3974 a         3653 c         2480 g         4737 t
ORIGIN      
        1 cttttgttgt attgccataa tacctgaaaa tgcaataact gcaataataa caggtataac
       61 atattttgtt acgttgtacc acaattcgaa taatccaaat tctattgttc cgccatttgt
      121 aagctcatcc ttcaggtctt ccttcttcat aaagaatccg acacatattg caaggaggcc
      181 tcctccgaca gccaggaata ttttgtcagt aaggatatca aagaagtcaa agaagccgac
      241 tccaaaaatc gtaaatcctg acataactcc cattgacaat gaagaaagca tacacattac
      301 cgccatgatt gcagaagtaa ggtaaacagc ttttttcctt gccattttat gctggtcaat
      361 cagatatgca acaacaactt ccagcaggga taccgatgaa gtaagagcag ctataaacag
      421 aagcaggaag aataatgctg agaatatcgg tcctatgccg cccatctgag caaatatctg
      481 cggtactaca acgaaaacaa ggccggggcc tgctgtcggt tctacgccaa atgaagcgag
      541 tgcagggaat attgcaatag cagccagaag agcaacgctt gtatccatag tggttacaat
      601 caaagcattc tgcggcagct tttcttcttt attcagatag cttccgtatg taatcatgca
      661 gcccatgcca aggcttagtg agaagaatgc ctgccctaat gctgccagga aggtctgacc
      721 tgttaccttt gaaaagtcag gtgtaaagag gaacttaaat ccttcgctcg cacccggaag
      781 gctggcagtt ctgaaaatta ttgctgcaag aagaacatac agcataggca tcataacttt
      841 gccggctgct tcaataccgc tttgaattcc ttttgcaaca atcaatacat taattaccag
      901 gtagattacc atccatacaa gcggctcaac aggtgcactt ataaaggcgc caaatgcatc
      961 tcctattgct tcgggtgcag ccagcaatcc tgtaaaggat tttgcgatat aagccatcgc
     1021 ccatccgcca actaccggat aaaaccccat aatgaagaat ccgctgagaa ctcccaacgc
     1081 tccggcgaag ctccatttgt cactgacttt tttgtatgcg ccgactgctg caaggccgct
     1141 tcttctacct acggcaaact cggcggtcat aatagaaagt ccgataagtg ctacacaacc
     1201 taaataaata aaaataaaag ctgcaccgcc gttcattccg gtcaggtacg ggaatctcca
     1261 gatgttaccg agtcctacag ctgaacctgc agctgccata agaaacccaa accgtgatcc
     1321 ccaattttct cttttttcag tcattaatta aacctccttt atgttatatt aataatataa
     1381 tacattgatt tttcaaaaaa ttcaacaatc attatgtatt atttttaata agatgaaata
     1441 aattagcatt attgatactt ttatctattg aaatttaagg ctttttatac attatgtgtt
     1501 attaatttaa catttcaaat catataatat atattatata ttcctgctgc ccggttttac
     1561 tgctttggaa cctgacttgc acatggggca atctcccggc tcgaagacct ctatatcgat
     1621 ttttattgca cttttaaaag gtacatcgaa taaggccttg ccgccgcttc tgtccactac
     1681 cgcggcaact ccgaccactt ctccccctgc agagcgcaca acatcaatca cttctttaac
     1741 tgagcctccc gtagtgatta catcctcaac caccagcacc tttgagccac gctgtatttc
     1801 aaagcctctt ctcaaagtca tgataccgtt ctctctctcc gcaaaaagtg cccttacctc
     1861 cagctgcctt gcaacctcgt atgcaagtat tatccctcca acagcagggc ctattactat
     1921 atctatatca tagccgtcgt atttctcagc cagatccttt gatatctcgg cactgatgtc
     1981 ggggtgctgg aaaacctttg cgcactgtaa atatttattg ctgtgcttgc ctgaggtcag
     2041 ctggaaatgt cccgtcagaa gtactcctgt cttttctaga attccattta ctcttttttt
     2101 tgtaagcact tgttatctcc tccgtatatt tttaagtttc ctttcaattc atttatattg
     2161 ctgaattggt tttttatgag gtatttttca ataccttcca aaatttccaa gggggcatat
     2221 ggatttatga agtttgctgt tcctactgac acggcgtctg cacctgcaag catgaactca
     2281 attgcatcct cccagcagct tatacctccc atccctatta tcggtatatc aactgcttgg
     2341 gctgcctgcc ataccattct tacagctacg ggcttcactg cagggcctga cagccctcct
     2401 acattgtttg ccagtacagg ccttcttgaa ttgatatcta ttgccattcc gagaagagta
     2461 ttgatgaggg ataatgcatc cgctcccccc tctttggctg cctcggcgat ttcctttatg
     2521 tcggttacat tcggtgacag ctttactaca agcggtacct tgcattgttc ctttaccctt
     2581 ctggttacat tatacaccga ttccgaattt attccaaatg cagccccgcc ttccttcaca
     2641 tttgggcagg atatgttcag ttctattccg tcaaccaggc ttcccagcct tcgggccatc
     2701 atgcagtatt cctcaacggt attgcctgca atattcgcta tgactgccac atcatatccc
     2761 cgaaggaacg gaagctcttt ctcaatgaag tactccactc cgggattctg cagcccgaca
     2821 ctgttcagca tacctgccgg tgtctccgca attctcggag gtttgttgcc ttctctgggt
     2881 tccagtgtca ggccttttac tacaattcct ccaagctttg aaagatcgta gaacccggag
     2941 tattcccttc cgaagccgaa agttcccgag gatgtgagca cagggttttt tagctttata
     3001 ccccctatat ttacgctcat tctagtcatc aaaatccacc tcctcagccg caaataccgg
     3061 gccgtctgtg caggcctttt tataatgcca tgtatcccct tcttttatcc tgcaggcaca
     3121 caccaagcac gctcctatgc cgcagcccat tctttcctcg acggatacaa agcatttcac
     3181 accggtgtcc tcacacagct ccttgactct tttcagcatc cctttagggc cgcaggcata
     3241 tataacgttg tatttcgtta tatcctttat aatatcggta acatagccct tgcttcccgt
     3301 acttccgtct tctgttgcaa tataaagctc tttacaggct gaagagaatt catccagctt
     3361 ataggtattg caccggaagc ccaaataggc atcacaatcc ttaaggctgc aagcaagctc
     3421 caataacggt gctataccga ttcctccgcc tactacggca cattttccct cgtcagacaa
     3481 agtaaagccg ctgcccaaag gccccagggc atctatgtat tcacccttct taagagcggt
     3541 catttctttt gtccctttgc ctacgcactg gtaaacaatt gaaatgacac ccgtatccct
     3601 gtaaatcctg cttatgctta taggccgcct taaaagcgga tcaattgact tgttaacctt
     3661 taaatgcagg aattgccccg gttgggcttc ccgtaccatt tccgggcact ctatcttcat
     3721 ttcgaataca tctttgcata gttccctgtt ctcaacaatt ttacatatgc actgcatata
     3781 cgcttcatct cctaccacgc tattttacct gtcccggcaa gctcattgtt tatgtcatgc
     3841 ctcattgcta ttgcggcttc ccttgccgca agtccgaact ccttttcact gaacctttcc
     3901 ctgtatgctt cactcttata ggctgcaatt atgcctcttg aggagttgat aacagcacca
     3961 agcccatcat cattaaagca tacggcagcg tcctctgcag taccgccctg ggctccatag
     4021 cccggaacca ggaaatatac ccctttcatc agttctctga gcctttgccc ttcgctcctg
     4081 aaggtggccc ctacaaccgc tccaaccgag ctatagccgt atttgcctgt cagcccttcg
     4141 ccaagtctta ctaccagttc cgccacatgc tcataaacct tcttcccacc cgactccagg
     4201 tcctgtatat ccttggatga cttgttggag gtcttcacca gcacaaaaat ccctttgccg
     4261 ttcttttcgc agttatttac aaaaggaatc attgagtcgg tgcctaaata cgggtttatg
     4321 gttatgcaat cggtttccgc cgaagcttta ttatttccaa gataaaaatc agagtaagcc
     4381 tctgccgtag aacctatgtc gcctcttttc acatcgccga taaccaataa accctttgat
     4441 tttgcgtatc tgcaggtttt caaataacat tcgaaacctg cggtgccgta ctgctcataa
     4501 aaggctatct ggggcttcac agcgggaaca atgtcatata cattgtctat tatctcttta
     4561 ttaaacctga aaaacgcttc ggcgataacg gaagctctgc tgccttccgc aactgcttcc
     4621 acggcttcat cgattaaata atcaggcaga tactgaagcc ttggatccag gcccacaaca
     4681 gtatgattcc tgcattttat aactttctct atgagtctgt ctgtaaacaa tcaaatccct
     4741 cctaaagccg atttttattt tcataggcta cattgccgtc aactattgta tatacaatat
     4801 ctcccttata tgtcttcccg tgaatgggag tgtttttacc ttttgacaag aaatcattcc
     4861 gattaaccgt aatttccctt gcggtatcaa ccagtaccaa atcagcagca atgcctgttc
     4921 tgattattcc tgtatccagc tttaatattt ctgccggatt tttgctcatt ctttcactta
     4981 attctttcaa tgtaagtatc ccgggctcta ccagttcggt ataacagacg gaaaatgcgg
     5041 tctccaggcc tgatattcca aaagcagagc tgtagaaatc ttcgcttttc tcatccttat
     5101 ggtgaggtgc atggtcggtg gctatcacgt caattgtgcc gtctttcaat gcttccttca
     5161 gtgcctcaac atctccggca gtcctaagag gcggattcac cttgcagtcg gtaaaacccg
     5221 gtgtgataat atcctccgtc agtccgatat ggtggggcgt cacttcacat gtgaccttga
     5281 cccctctttt ttttgcctcc cttattgttt ccgcggatcc ggcagtgctt acatgggcta
     5341 tgtgtatatg cgagtccgta tttccggcaa tcataatatc ccgcattata ttaagctcct
     5401 cagaaagggg atgtatcccc cgtatgccca tctgctccga cttccttccc ttgtttattg
     5461 cgccgccctc ggccaggctc agttcttcgc agtggtcaat tatcataaaa cctttttcct
     5521 tggcgctcaa cagtgccttc atcataaatg ctgccgtggc caccggcctt ccgtcatcgg
     5581 aaaaagcgac agcacccatt tctgagagct tgttcatttc aacaagctca gtgcttttct
     5641 ggcctttgga tatggcagct atggggtata ccttgactgc cgcatccttt tttatgattt
     5701 tttggagcat ggataaagag gcttcatcat caattaccgg cctggtgtta ggcatgcagc
     5761 agattgaggt gtatcctccc cttgcagcgg ctcttgtccc cgaatatacg gtttccttgt
     5821 attcaaagcc gggctccctc agatgcgcat gcatatcgat gaatcccgga agcagaagaa
     5881 gcccctccgc atctatcact ttatcggcag caagcccgat tgaaggttct atcagcttaa
     5941 ttaatccatc ttcaatataa atatcattaa tcccttcata agtatttccc ggatctatta
     6001 tgctgcattg ctttataagc aatctcatgc aattacctct ctttcataaa ataagaaaaa
     6061 agagcaataa aggtatatta ctcttgttct cccaaaagaa ctgcatcctt gccatccact
     6121 tctccgacat ttacaataat catttcactt ctcgaagtag gaacattctt ccccacataa
     6181 tcggctctta tcggaagctc cctgtgtccc cggtctacaa gtacagccag ctgaatgctc
     6241 aagggccttc caatttccac gatggcatcc attgccgctc gtacagtcct tcccgtatac
     6301 aaaacatcat caacaagcac tactttcatt ccgttgatat caaaggagat atcagtcaga
     6361 ttttcccggg gtatatcctt catatccttg atatcgtccc tgtgcctggt aatatcaaga
     6421 gttccgacag gaagcacatt gccttcaatg tttcggattt tctcacagag ccttttggca
     6481 agggagactc cccttgtctt aataccgagc agaaccaggc cttccactcc cttgtttctt
     6541 tctattattt cgtgagatat tctggtaagc gccctgtcca atgctttttc atccattatc
     6601 tctgtcttta tcctcataat gattcctcca tgctgtttta tagcaaaaaa tctgccgtga
     6661 ggcagattgc agttatattt ttgaatcctt ccatgcctca ccggacaagt ttaaaggttg
     6721 ctttttaata gattttacca tagcatatat aatttgtcta tattcttcca ttcctcaaaa
     6781 gccttaatac tttctcaaag tattccggaa tgggggcttc aaattcaaca tattccttct
     6841 tggccggatg aatgaatcct agtacccttg catgcagcag ctgtcctttt gtgtcaaagc
     6901 gctgtttttt atagccgtat actgtatccc ccacaacagg gtgccccaga tatgccatat
     6961 gcactcttat ctgatgggtt cttcctgtct ccagtaccgc ttcaatcaag gtatggttct
     7021 caaagctctc aagtactcta tagtgagtaa ctgccctttt cccgttttcc ctcacaacag
     7081 ccatctgttt cctattcttt gggtttctgc ctataagggt atccgctgtt ccgcattctt
     7141 ttttcaatct gccttcaagc agtgctgtat actttcttgt tattgtatgc tccttaagct
     7201 gtcccgataa ggaaaggtga gcttcattgt tttttgccac aactattata cctgatgtgt
     7261 ccttatctat cctgtgcact atccccggcc tcataacccc gtttattccg gtaaggctgt
     7321 tatagtcgga caggtttttg caatgtccca taagggcatt cacaacagtg ccggaataat
     7381 tgccatggga aggatgcacc accattccct gaggcttgtt gattactaca atatcattat
     7441 cttcataaat tatatccaga tctattttct caggctccac ttccaaaggc accggatcgg
     7501 gtattacagc ttcaacccgg tcaccttcac gaagcttaaa cctgggcttt gcattttttc
     7561 cgttgacttt gcagtgccct tcttcaataa gcttttgcat ataagttctg gaatagcctt
     7621 ccacctttcc cgacagaaag cagtcaagcc tctctccctc tgcttccggt cccgctatat
     7681 attcttttac ctccatacat caacacctca taatctcgaa acctcataat ccaaaaactt
     7741 caaagtcctg cccaaccctc gtaccccgta ccccgtatcc ccgtacctat atttttatat
     7801 ccttgccgaa tataagcagc aggctaagca gaattgcgcc caccactatt gcggaatccg
     7861 caaggttaaa aaccggcgga aatatagtga atgaaaggta gtcaataaca tatccgaacc
     7921 ttattctgtc tattacattg cctatcgctc cgcccagaat aagagacagg caaattgtaa
     7981 cggggacgct tttattacgg tacttgtaaa tcatgaatgc caccgctgcc gatataatac
     8041 aattggtgac aataaaaaac aataccttgt ttttaaacat gccgaaggct attccgtagt
     8101 tctccacata aacaaggtaa aagaagttct ttattaccgt tatgggtcct gaagccaaag
     8161 cattggccaa tatattcttg ataacctggt cggatactat aatcgctgct gcaatgagaa
     8221 aaggatacaa tgatattccc ccttaaatgc aaataagtac aacagttatt atagcatata
     8281 accctgtttc attccaactg cttcctgtac tgctcattac ttacctgttc gacatcttca
     8341 acataccctg ccgattcgtc ttctccaaat tcattatccg acattcttac attatattcg
     8401 ttcactgact cccaggcatc ttccccgtca aaatgtacct tatcctcaac cgaattatcc
     8461 gtaaaagtcc tgccaaaagg cggataaagc acctgctctt caataggcct gcccttctcc
     8521 tcattttgta cattcgaacc gtattttttt tcacaattga tgcataatct ggagtatggc
     8581 cttaattcca gcctgtcttt gccgatctca ttatgacaaa tctcacatga accgtatgtt
     8641 ccgtcagcta tcctgttcaa agctgcgtca atctcactta aaagaaattt atccctagtc
     8701 ctgaaggaaa aattcttttc agcctcaaag gtctcatttc ccatgtctcc gggatgatta
     8761 tcataagaag aaagctcacc ggtcagatct ttgacggaaa ttccaagccc tgtcctctca
     8821 tcttcaagtg agttttccct cttttttttc tcctccttca gcaattcctc aaagtgctta
     8881 aagtcattct tgtccaaatt ttctgcctcc ttttttattc atagatgatc ctgaactatc
     8941 ttgactattt ctgctatgaa ccctcctata aaaggaagat tcaaaagcac agactgtctt
     9001 agaatataaa tgacaacagc tccaagaatg gtgaagccta cagcagtgcc aaagcctctg
     9061 gctacccctc ctataaaatt attcaggagc agagttttag gcttggtcat aatattgaca
     9121 tactcgccaa ttttggcctt ttccagcagt agcgctattt tttctatcct gctttccagt
     9181 tccttcactt cggtattatc aatatccata cacatgcccc cttggccccc aatgaaaata
     9241 taaagccatg aataacttca tgactttata ttatcaacat tcctacataa atattcactt
     9301 tttaatcttt atatatcctt caaggttgca ttaagagcct caagctcaga ctccaacaga
     9361 gtcttgaacc tggtcttaaa gacctgtact tcctttttca attcctcaaa atccttatta
     9421 acctttataa cactgctgtt tgcttcttct attatctttc tcccctgctc ttctgcttcc
     9481 ttaataatca tttccgcttt tttcctcgca tttgcaacaa tgtcctcagc agttgtctgt
     9541 gctatcagaa gagtgctctg aagggatttt tccatgctgt tatagctttc tatcctatca
     9601 ttgtctatgg ctattctgtc ctttaactca ccgttttccc tgtaaattct ctcatagtct
     9661 gctacgacct tatccagaaa ttcatcaact tcatcttcct tgtatccttt gaaggccttt
     9721 ttaaattcct tgtttcttat atccatcgga gttatcatgt tccttcaacc tccatcacag
     9781 gtatttctta ataacaattc ctattcttcc tttttttgtt gtatcgtata ctactccagg
     9841 attattcttc acagaaccat tatttacagg aaagtatata tcaaagtcag cagtaaaggt
     9901 ttggctatat aataaagaaa taacaatgat attatgggtg aaaaatcaat catgcccgta
     9961 ttgagtccca atttggcggt aagttttcta aagggctcaa gtatcggatc tgttaactga
    10021 aatatcaaca ctattatctt actgtcggga ttaaaactga accaggacaa taaaactcta
    10081 atgagtatca taaagttaag aaaatcaata aaatatgaca aagttgcagc caagacaaat
    10141 ttcatctaac cactctcatt ctaaaattat ttaagccagc tgaaaattcc ttccaggtcg
    10201 aaattctcat cgcctataga actcttaata ttacccaaaa catccacatt cgacggagca
    10261 accagcacta taccgtttga aatcttttga attgtaccgt caagtacata taccgcgccg
    10321 ctgataaaat cgaccatcct tttggctact tccttgtcca tctcttcaaa attgattatt
    10381 acaggttttt tgcttctaag atcatcacag atggattgaa cttcatcata ggaagaaggc
    10441 tctaccagaa caaccttcat gggtgcagag gcagtatgta tattaactac attgctcctg
    10501 cggtgggaag cggtgcccac tgcttctatc tgaggttttg atacttcagc ttcttcgtgt
    10561 tcttcttcag cagcttctcc tattcccata aaataaagta ccttgttcaa gatattggac
    10621 tccattacta tacctcctta ttataaatca taattccttg ctccaaaaat acctgtccca
    10681 atccttacca tgttggaacc ctcttcaatt gcaaccttat aatcgttggt catacccatg
    10741 gataaatact tcatatctac atttggaata cttgctgctg ccagcatatc gaatttttct
    10801 ttcagccgtc taaacacagg ccttgccagc tcagcatcgt cataatatgg ggcaatggtc
    10861 ataagcccct tcacccttat gctggaaagc cctgataacc tctctgcaaa gctgtctgtt
    10921 tcatcatatt ctattccgaa tttggtctct tcctgtgcta cattcacttg tacaagcaca
    10981 tcttttacaa gcccggcctt ttcagccctc ttatttattt cttccgccag gcctatgctg
    11041 tcaacagaat gaatcaatgc tactttatct attatatatt ttaccttatt ggtttgtaaa
    11101 tgcccaatca ggtgccaatt aacattttct tcaatatttt catattttgc cattatctct
    11161 tgcactttat tctcaccgat atttctaatc ccgcatttta ttgcataatt cacacggctg
    11221 ggatctatgg tttttgtcac ggctattatg gtaatattgt caggggcttt gccttttttt
    11281 atacaaatat catttatatc ccgggatatg gcaataatat tatttttgat ttcctgcaaa
    11341 gttaaacccc tcctatattt tatcggtaaa aacctgattc tccttaaccc tttctgttgt
    11401 agtaattact tcgtcaaaaa ttttcagagg ctcatattcc gatttggaat tccattcttc
    11461 cttattaaga tttctaacga ggcatctttc tttatcttca ttcataacag caacaggtat
    11521 gaattttaca attccttttt taagtacata cagccctctc gtattatcct taaccactat
    11581 agcgtttgcc gacacttcga agccttcgtt ataatccttt attatatcaa cctcggcaat
    11641 tctctttttg taaaaatcat tgacatgatc gctaatcctg attattacca tgcatatgtc
    11701 accttccggc tgggatacct catgcacctc gccgttaacc tctgcattcc ccagctctga
    11761 aaatctcagt ctgacttttt ttcctgcctt tatatccttt gtctgatcga agcttattaa
    11821 actgcatgtg taccattcaa aattgtcaac tatcttaatt ccggccgtag tgctctgact
    11881 ggttttttgg ctgtcagccg tcaaagaact cattatttct ctgatattgc tgagcttgaa
    11941 atccttgata ttcccaatat tcagtatctg ctcataaccg tccaggcttt tgcttacaac
    12001 tcctgaagcc tgggcatata ttacatcaat actgttattg tatattccct ccaggacact
    12061 tttttccttt tgcagctgct ccagattctt cccgaaaaag cttccttctc cgtatatcaa
    12121 agattttttg tataaattgg cggcaaggtc gttttttaca tcatcaagct ttttaaggtc
    12181 tcctgacctg gcgatgctct taaggtcgga caccctttcc tttatccggt tctctatctt
    12241 ttccttgtcc tttgaaaaaa agttgttatt aatatccgac tgttttattt cttgtatcct
    12301 gtcatccaat tgctttatct ttattgatat attttcgtcc gcctgatcat tcttcacaat
    12361 ctccagaatt ttcttcccca gggagacccg ttcgccttca ttgacattat atttaatttc
    12421 cactcccgac ggaagtattg tagttgattc attccttatt atcaatgcct ttactcttat
    12481 tgaattctca actgagccat attttactat ttcagtttca atattgaaaa acatgaatct
    12541 aataaagtat gatagacaaa taacaaaaaa caatacaggg aatatataaa cagatgatat
    12601 tttatttttt tttgccctca tagcaacagc agctcctcgc aagatacagg tataacttat
    12661 tgaactttat atttacataa ggtatatatt tctccaccgg ttcccataat cctgcataaa
    12721 ttaagcaaat aataaaatta gtgccagctt cccattgaaa tagtcctgaa ggtaataatg
    12781 caatgtgtga ataataatca atatgcaact taatattgcg tgctgttgtt tttgatgttg
    12841 tattttacga agaatttgta tataatatta tcaacaagaa gagaggtgat attatgccaa
    12901 acatcaaacc agtttctgat ctgcgaaatt ataacgaggt cttaagagat gttgcggttg
    12961 gcgagccggt ctttttaaca aagaatggca gagggcgcta tgcacttgtt gatattttgg
    13021 actatgaaaa agctcaagcg acaatcaaac tgatgtctga gcttgcaaag ggacaaaaat
    13081 caggtgagga aaaaggatgg ctttcacatg aagcagcgaa agcacatttt aaagaaaaaa
    13141 acaatgaata agctatacta ctctccaaag gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnccggcaaa atatgttctt gaaatttata cttttttatg atatattcaa
    13321 taggtgtcag aaatgatata agaatatttt tctgggtgtg gcgcagcttg gtagcgcgct
    13381 tgaatggggt tcaagaggtc gcaggttcaa ttcctgtcac ccagaccatc taagtaatca
    13441 aagtgcttta aggcactttt tttattttgt ataaaaagct gtatcaggct tgtcaagact
    13501 ttttataacc acaaacgcac caaatatata ctgccttaat actatatatt aagaaaagca
    13561 gacaaggtat cccaaaagga ggtgtataaa tgagtaaatc atttagaaaa ccagtttttt
    13621 catctcgttt caaccggttg ccggacagta cagtagaaaa atgcggtgaa tgctgtttag
    13681 ttcacgaaaa gttttcccta aacctgataa aaaaagcaac aaataaacca agtacaaata
    13741 caatctttaa ggcaagtgaa tttcaagatt gttctccggt acaaattggt atagcacaaa
    13801 attcttcccg tttcccattt ggcagtatat tggtaagaaa tttttcagca gtacccgtct
    13861 cctttacagt atttggggat acggaagaag aaaccgatat gacagttcaa tcaaattctg
    13921 aggttgcagt aacattaaac gctataagtg cagtagagat attttaccct actgacccta
    13981 taagactgct gttcatattt gatatattcc atcctggtga cccaattacc cctccgaacc
    14041 cataataaga cgttcaccct gaatataatc gtaagttaaa agcctgtaat cgcaacaatt
    14101 acaggcttgt tttatgggtt tttccatatc aggaatctca gcattctttg acataagttc
    14161 aaaatgtatg agtatttttt aaaataggtt gggtgttctt ccctcctctc tccgtaggtt
    14221 gtaatattta gtccgtaaac atccttgttc atcttcctta acagtgaaga gactgatgtg
    14281 ccgctgcagg tagcaaggct tatcaggaaa agttcaaggg aggtatatcc ttcaccgtct
    14341 ccgataggct ttacatagtc gataattatc tcctttttgt cccttgaaac acccgagaac
    14401 ttcaccttat catttataag tgctgcaact gctgttaatt gattgctcat aaaaaaacct
    14461 cctgttattt tctgatttat aagcttagat actaaaaata acaaaaggtc acatcgatta
    14521 tagattatta cgataaaatt tctaaagatt ttttagaaat catagtttta atattttcaa
    14581 aaaaagctgc aggcattgtt ctctcataga attctctcct tcagcaatag taaaaccttt
    14641 tttacaattg aagctcctca ggctgtctaa gacgctgatg cctcaggacg ggaatctgat
    14701 acttctctcc aaccgcacgc accaaatcct tatagcagcc tttcattttg tcatggagca
    14761 ccaccgcctc cacgccgcct gcaattttat caacagcagc acttgtaaat gcaagcccat
    14821 gctccttttc atactcccct gcaacgagcc actggttggc tatgatatgt gaaacaccga
    14881 atgcatcatc ttcccattgt gtaaatacgg cttgtgcaaa gtttggatcg aaatggatac
    14941 ctcc
//
LOCUS       DAOQUR010000056        14469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_4718_length_14469_cov_10.154433, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000056 DAOQUR010000000
VERSION     DAOQUR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4718_length_14469_cov_10.154433"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..213)
                     /locus_tag="PKI50_10005"
     CDS             complement(52..213)
                     /locus_tag="PKI50_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04074.1"
                     /translation="MDNNRVLLSNLKAYHKLEKLLKIQEALDSNKIIQKAKANDAKVE
                     SCTASNPII"
     gene            707..958
                     /locus_tag="PKI50_10010"
     CDS             707..958
                     /locus_tag="PKI50_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNT04075.1"
                     /translation="MYISKIQKWGNSQGVRIPKYILDHADISEGDNVAISLEDNKIIL
                     FRPNKALKKYTLEELFKDYKGDYKPEEYDWGVPVGKEEW"
     gene            962..1288
                     /locus_tag="PKI50_10015"
     CDS             962..1288
                     /locus_tag="PKI50_10015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNT04076.1"
                     /translation="MSYVPKQGDILWITLYPQLGHEQKGRRPGLVISNDTFVKMTGLI
                     LLCPITRTDNGFPLHLKLSDYNTCGYVMIEHVKSMDYNQSDVEYIETLRPEDLVEVLS
                     VLNACF"
     gene            complement(1285..2208)
                     /locus_tag="PKI50_10020"
     CDS             complement(1285..2208)
                     /locus_tag="PKI50_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT04077.1"
                     /translation="MRKRMIQDPAAAIVDAAKSDNCVEDVLNMIEAPKIIGQGACVVI
                     TANMVDNKPASTGTVVHPDLLRKVIRYAKKFNPSRIVVAAGSGGAPTQQVFEEIGFNR
                     IIREEEIEFMDLNYGPYIELKLNHDIIPSIKINKLCEEMDVHISFTPIKMHKEATVSL
                     GIKNVALSWPPAEIHGMPKKKLGIHEDLHNFIAAMGEVVPIDITLLSGMNGMIWTGPS
                     DGKAVSSNLIVAGTDPVAADAVAARMMGLLPQAVHYLFELHRRGLGQADIKKINMKGV
                     PLVQAEEMFSMAALGESIAIDRPRILTFHGD"
     gene            complement(2251..3243)
                     /locus_tag="PKI50_10025"
     CDS             complement(2251..3243)
                     /locus_tag="PKI50_10025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT04078.1"
                     /translation="MFEIRLTENEAGQRADRFLRKYLKEYSLGDIYKLFRTNKVKLNG
                     KRVKENQMLKEGDLLQLYVEKPERAAKTAIPLVKPVGGIDLIYEDANIIIADKPAGLL
                     THPDKPGDTDTLLDRALYHIYGEKEADESRTFSPAVCNRLDRNTSGIVIIAKNYKTLK
                     AVNHSIRERGIRKLYLCIAAGKMDKSGEIKGFLEKDRNRNIVYVKENGTGDGKQIHTI
                     FRTLAVSESIRELGRQFSLLEAELVTGRSHQIRAHFAYLGHPIAGDVKYGDKTINEYF
                     RKRYGLKHQFLHAHKVAFEEVHEDISYLKGRSFHCDLDQTAAMTAASLFGTDWV"
     assembly_gap    3303..3402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3445..3786)
                     /locus_tag="PKI50_10030"
     CDS             complement(3445..3786)
                     /locus_tag="PKI50_10030"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT04079.1"
                     /translation="MQSVIRNQKDVEPVKNPKHNEVYGRLLAKQEENGRLSAHLTNIK
                     PGGEILPHTHEVLEVFYVIEGEGLALVNGVRQKAEKGTIIAANAGSEHGIINTGESDM
                     VLFCVFSPGIA"
     gene            complement(3801..4214)
                     /locus_tag="PKI50_10035"
     CDS             complement(3801..4214)
                     /locus_tag="PKI50_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNT04080.1"
                     /translation="MGKTYEQINVGDKGSFEKTISETDIYMYAGVTGDINPAHINHEA
                     MKNTKFGGRIAHGMLTAGLVSAVLGMQLPGPGTIYLSQQLNFKAPVKIGDTVKAVAEV
                     VEKLEKGRVRLKTSCYNQDGVEVLDGEALVIAPRE"
     gene            complement(4313..6913)
                     /gene="clpB"
                     /locus_tag="PKI50_10040"
     CDS             complement(4313..6913)
                     /gene="clpB"
                     /locus_tag="PKI50_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNT04081.1"
                     /translation="MDINKLTQKAQEAVIESQSLAIKHNHQTVEGEHLHYAIILQNEG
                     LIPKLLSKMNVPFDFYYKDISDLLDKIPKIYGETASSPYVSRRFEQLFLRADEEAKKF
                     QDEYISVEHIYLALIDETNTPSSKIIKKYGITREKFLRELSAVRSNQRITGQNPEETY
                     EALKKFGRDLVEDARKGKLDPVIGRDNEIRRIIRILSRRTKNNPVLIGEPGVGKTAAV
                     EGLAQRIMKGDVPEGLKNKTIFSLDMGALIAGAKYRGEFEERLKAVLKEIEKSNGRII
                     MFIDEIHNIVGAGKAEGAMDAGNLLKPMLARGELHCIGATTLDEYRKHIEKDAALERR
                     FQPVLIDAPDVEDTISILRGLKEKFEIHHGVRIQDNALISAAVLSNRYISDRFLPDKA
                     IDLIDEAAAMLRTEIDSMPGELDEIMRRIMQLEIEREALKKERDKQSEERLEALNREL
                     SELKSKGDSMKAQWELEKENIKNIREIKKRIEETKAEIEKAEMKYDLNRVAELKYGTL
                     IELEKQLNAEKANLEKMQGMGQLLKEEVTEEEISDIISKWTGIPVKKLMENEKEKLLR
                     LDDILHKRVIGQDEAVSAVADSVIRARSGLKDPKRPIGSFIFLGPTGVGKTELAKTLA
                     EALFDSEDNIIRIDMSEYMEKHTVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVLLFD
                     EIEKAHHDVFNIFLQILDEGRLTDSQGRTVDFKNTVIIMTSNIGSMYLLEGVDNDGEL
                     KPEAVEAVNEELKRHFRPEFLNRVDDVVFFKPLLKEQIRKIIDLIFEQVAERLKDRNI
                     KISLSENAKDYIIGNSYDPVYGARPLKRFIQRKMETSIGRALIAGEINDGNDILIDAD
                     EKELKFKKLS"
     gene            7073..7273
                     /locus_tag="PKI50_10045"
     CDS             7073..7273
                     /locus_tag="PKI50_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HNT04082.1"
                     /translation="MARNNQKVVPQATAALDRMKFEVASEVGVNLKEGYNGDISARDA
                     GRVGGTMVKRLIEQAERSMSGR"
     gene            complement(7349..7984)
                     /locus_tag="PKI50_10050"
     CDS             complement(7349..7984)
                     /locus_tag="PKI50_10050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT04083.1"
                     /translation="MSVATKSYSVVNPYHLKKLLRVRKAEISDINSIYDVASSVGTSE
                     KISEQGFLVDDYQSKPNYYKKKIGHWIENLERFYVAEYDKVYGFMIAYTKEEWLRDNP
                     SWLQDVYWNPEFDPSWTNKFIVVDKTAVFADLTGMGIGSLIYKRLLEDLRNEGIKHIF
                     AETIISPQPNFASLQFRKKQKYSLAGVRYEKYNNILYTDLIYHKSAFDALT"
     regulatory      complement(8091..8225)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(8310..8729)
                     /locus_tag="PKI50_10055"
     CDS             complement(8310..8729)
                     /locus_tag="PKI50_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNT04084.1"
                     /translation="MKMPEFSIGMSKSVQKVITREDTALNFGSGALKNLLATPTLSAL
                     MIEATVKTIDPLLPDRYITIGKSIEIEHEHATKEGTTVTVSAELVEINGNVLSFKITA
                     FDEIGRIGTGYHVRQIVNYDILMKRVEERFSVMEGRP"
     gene            complement(8890..9321)
                     /locus_tag="PKI50_10060"
     CDS             complement(8890..9321)
                     /locus_tag="PKI50_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNT04085.1"
                     /translation="MLTVNSINCGIVIDHIAAGNGIKIFEKLKLDEVDFPVVLLINVP
                     SRKLGRKDIIKIENKIDVDLTMLGLIDPNITVNIIENDKIVKKMNTIIPEEVQGLFKC
                     KNPRCVSNFDEYVTPKFHLAKANGKIHYRCGYCEELTTYSI"
     gene            complement(9305..10249)
                     /gene="pyrB"
                     /locus_tag="PKI50_10065"
     CDS             complement(9305..10249)
                     /gene="pyrB"
                     /locus_tag="PKI50_10065"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNT04086.1"
                     /translation="MSLKGRHLIDPNDFSLSELDEIFDLADRMADDRDSFGNICNGKI
                     LGTLFYEPSTRTRLSFESAMLRMGGKVLGFSDAGTSSVSKGESIADTIRVLSSYADVV
                     VMRHPKEGAPKLASEYSLIPVINGGDGGHQHPTQTLTDLLTIRRYRGTLENQTIAFCG
                     DLKFGRTVHSLISAMSYYKGSKFVLISPAELRLPGHLKSSLIEKHNVGIYETENLEEV
                     INQVDVLYMTRIQKERFFNEADYMRLKDSYILSRSKLKDSREDMLVMHPLPRVNEISY
                     DVDDDKRAVYFKQAEFGIYVRMALMALLLGVNVNVNRK"
     gene            10561..12786
                     /locus_tag="PKI50_10070"
     CDS             10561..12786
                     /locus_tag="PKI50_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT04087.1"
                     /translation="MLKKKLAALGIAVVFTLSSLTTTVAADYKKPAAGGNITIKVVSP
                     GKGNAYGQTKIKKNEVKKYRFDDCADITWALNAIEKLAGKGIIGGIGNGKYAPRSNVT
                     HVEALAMVMRLTGDDKEAEGMGKKVHPFYKGILPNWGYGYIFMAIEKGILLPEELKDF
                     NPNQPAKRHEIAKYIIRAMGETKEALKHMDEDLDFKDSSAVPDKSVGYVYLVSKLNIM
                     IGNEKNMFKPMEPVTRAEMAVLLDRAEGKFDLPDTDNRKNSTVFVSADVDDNEITVKV
                     KGVTVTYDVLEDVQVYRDGSFETIEDLVKGDILQLTLNNSKDVIFIEVVKSAEEGDDD
                     EEEEIAVSFRAVSDSSLPKVLKDEIDDMKSKESYAAYEYDNYIYLVAAMGRQRTGGYD
                     IEIEKVYRIGDNDEYTIRAVVDTDEPSSGSNVTQAITYPYSVVKFKSFDDIEEVIFVD
                     EDDDELDDVEIEDLDEDPEETKVSFAEVDYDDLSAVLKDQVDYLKLNKNFKAYEYDNI
                     IYLIATMGKKNSEDYKISIDDVYKVENGDKYTIKAVVDTTVPASSSGSKSVYPYSIVK
                     LRNFDGIDGVRFVDEGNNKLSETKIVELDEVVTAEGIISAINTSGKQIRVKKSNGTTV
                     ALTIPSSAEITVNDDDDASISDFEVGMKVKVDIVGDIVTEVIAEDDKTEVTGTLTGIS
                     ISTVKKITVKVDNTTKTYTVDSDVKVIIDDETERVEDLRVNDELTIKFINGILAEIEK
                     K"
     gene            12909..14441
                     /gene="spoVB"
                     /locus_tag="PKI50_10075"
     CDS             12909..14441
                     /gene="spoVB"
                     /locus_tag="PKI50_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HNT04088.1"
                     /translation="MKKLTYIQGAFILTITNLITGSLSFIYRVFLTRAIGSEGVGVYQ
                     LVLPLYLLFITLVSGGITTAVSKLIAEQAPGSNYRNMYKIIKICGITIGIWSLALCSF
                     IFFNSEYLAKYVLKDNRTFFSIMVFTPAIFFISLSAVLKGYFYGLQYVKPPAMIDILE
                     KMIRLGGLLMTTYYLLPLGIEYVCAGVMAAMAAGEFISLILLYFCYKVKKAAVVHTKT
                     DSTIHIITRILKTALPLSLSGSLTTIMDMITAILIPMQLRAAGYSNSSALSIYGQVTG
                     MVMPLLFFPFIIVASLGITLVPAIAASYAAGNWTALNKKCNDSLKITSAIAFASTALF
                     IVFPWELCRIFFKCPEAGNMLFWMSFVCVFEYWQFTLFAVLNGVGKQHVVLKNSILHI
                     VISVICSYFLIPIPSIGIYGFVIGFCASAVLICLWNLADLKRIPNIHIDYVNIFMKPL
                     FAFVGMLIVIKCCNLYLVARTLQNFNMTVSSITGLVFFFLILFFMGIFTPKQLRNTFA
                     MK"
BASE COUNT         3908 a         3259 c         2693 g         4509 t
ORIGIN      
        1 gtcccatcca tgccccatgt aaaaaaatcg acttgcgtcg attttttcgt tctagattat
       61 cgggtttgat gccgtacaac tttcaacttt tgcatcattg gcttttgctt tttgtattat
      121 cttgttgctg tccaatgctt cctgaatctt caatagcttc tccaacttat gataggcttt
      181 caggttcgaa agtaatactc tattattatc cataattcca cctctttctt tcaaatttgg
      241 ttgtgataat aataaatttt tgctttctct cttgatcaac acctccaaat gatatataaa
      301 gctatttcaa acacaaccta aattcgtaac ttatttctca tataaataac gtcgacgttt
      361 atcttaattc tattataacc tatttctttt acaatttctt gcaaaggaac cctgccaaaa
      421 tgcgattaaa cattcctatc catacctatc cgcttttatc agagatagcc tataaacttt
      481 accggaactc ctccgtattc ggcagttttc cttataggtt taataaggag tgtgttctat
      541 ttaagcattt gagctaggtt aatatggttc tatattttgc tttttatatt gacatattat
      601 ttttaaaagt atatacttta tatatacatg ataaaaggag ggattatatg attgtgcatg
      661 gcagataata taaataatat aatttaaaat tacggagtga tttataatgt atatttctaa
      721 aatccaaaaa tggggtaata gccaaggagt caggatacca aaatatatat tggatcatgc
      781 tgatatttct gagggtgaca atgtagcaat atccctggag gacaataaaa taattttatt
      841 cagacccaat aaggcattaa aaaaatacac cttggaagaa ttatttaagg actataaagg
      901 tgattataaa ccggaagagt atgactgggg tgtaccagtc ggcaaagagg agtggtaata
      961 aatgtcctat gtgccaaaac agggcgatat actttggata acactgtacc cgcagcttgg
     1021 ccatgaacaa aaggggcgca ggccgggttt ggttatcagc aatgatactt tcgtaaaaat
     1081 gaccggattg atattactct gtcctataac aaggactgat aatggttttc ctttgcatct
     1141 taaactgtca gattacaata cttgcgggta cgttatgata gagcatgtta aatcaatgga
     1201 ttataatcaa agcgatgttg agtatataga aacattgagg ccggaagatt tagttgaagt
     1261 attatctgta ttaaatgctt gcttttaatc tccgtgaaaa gtcaatatcc tcggcctgtc
     1321 tatagctatg ctttcgccta gtgctgccat gctgaacatt tcttccgcct gcacaagcgg
     1381 cacgcctttc atatttattt tttttatatc agcctggccc aaaccccttc tatgcaattc
     1441 aaaaagataa tgcactgctt gaggcagcaa gcccatcatt ctcgccgcca ctgcatccgc
     1501 cgccaccggg tctgtaccgg ctacaatgag attagagctt acagccttgc cgtcgctggg
     1561 gcccgtccat atcatgccgt tcatccccga gagtaaagtt atatcaatcg gtacaacctc
     1621 acccattgcc gcaatgaaat tatgcagatc ctcatgaatg cccagcttct ttttcggcat
     1681 tccgtggatt tcggcgggag gccatgaaag ggccacattt tttattccca gggatactgt
     1741 tgcctccttg tgcattttta tcggggtaaa ggaaatatgt acatccatct cctcacacag
     1801 cttgtttatt ttaattgatg gaataatatc atggttgagc tttagttcga tataaggtcc
     1861 gtaattcagg tccatgaatt ctatttcttc ctcccgaatt atcctgttaa atccgatttc
     1921 ctcaaaaacc tgctgtgtag gagcaccccc ggagccggca gcgactacaa tccttgaggg
     1981 attgaatttc ttggcatatc ttattacctt tctcaataaa tcaggatgaa cgacggttcc
     2041 cgtactcgca ggcttattgt ccaccatatt tgcggttatt accacacaag ctccctgccc
     2101 gattattttt ggagcttcta tcatattcag tacatcttcc acacagttat ccgattttgc
     2161 ggcgtcgact attgctgccg ccggatcctg aatcattctt ttgcgcacaa aaaaacacct
     2221 cccctaaatt ttccccccgg caggtgtttt ttatacccaa tcagttccaa acagcgaagc
     2281 agccgtcatt gcagcagttt gatccaaatc gcaatgaaaa ctccttcctt taagatagct
     2341 tatatcctcg tgtacttctt caaaagcaac cttgtgggca tgcaggaatt gatgcttcag
     2401 gccgtatctt tttctgaagt attcatttat tgtcttgtcg ccgtatttca catcacctgc
     2461 aataggatgg cccaggtatg caaaatgcgc tcttatctga tgggaccttc cggtcacaag
     2521 ctctgcctcc agaagtgaaa actgcctgcc caattccctg atgctttccg atacggcaag
     2581 ggtcctgaat attgtatgta tctgcttccc atctccggtt ccgttctctt taacataaac
     2641 aatgttcctg ttccggtctt tttccagaaa tccttttatc tcgccgcttt tatccatttt
     2701 tccggctgca atgcaaagat agagctttct tattcctctt tccctgatgg aatggtttac
     2761 tgccttaagt gtcttgtagt tctttgctat tatgacaata ccgctggtgt ttctgtccag
     2821 cctgttgcac actgccggtg agaaggtccg ggattcgtca gcttcctttt caccgtatat
     2881 atggtacaaa gccctgtcaa gaagagtatc ggtgtccccc ggtttatcag gatgggtgag
     2941 cagtcctgca ggtttatcag caattattat attggcatcc tcatatatca aatcaatgcc
     3001 tccgaccggt tttaccaagg gtatcgcagt ttttgcagcc ctttccggct tttcaacata
     3061 cagctgcagc aggtctccct ctttaagcat ttggttttcc ttcacccgct tgccgtttag
     3121 cttgaccttg tttgtcctga aaagcttgta tatatcccct aatgaatact ccttcaggta
     3181 ttttcttaag aacctgtctg ctctttggcc cgcttcattt tcagtaagtc tgatttcgaa
     3241 catattacac ctctgcaatt tatgttatcc ttatattttg cgctatcatt ccggaattat
     3301 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacaaata tgggctataa
     3421 gtattaactt atggcccaca gcttttatgc aattcccgga gagaatacgc agaacaacac
     3481 catatccgat tctcccgtat ttattattcc atgttcgctg cctgcattgg cagcaataat
     3541 tgtacctttt tccgcctttt gccttacacc gttaaccagc gccaagcctt ccccttcaat
     3601 tacataaaat acttcaagca cctcatgggt atggggaagg atttctcccc ccggcttaat
     3661 attagtcaga tgtgcggaaa gcctgccgtt ttcctcctgt ttagcaagca gtctgccgta
     3721 tacttcgttg tgctttggat tttttaccgg ttcaacatcc ttttggtttc tgataactga
     3781 ttgcataaag tcccttcctt ctattctctt ggcgctatta ccagagcttc gccgtccagc
     3841 acttcaactc cgtcctggtt atagcagctc gtttttaatc ttactctgcc cttctccagc
     3901 ttttctacaa cctccgccac agcttttaca gtatctccta tttttacagg tgctttgaag
     3961 ttgagctgct gtgaaagata tatggttccg gggcccggaa gctgcattcc cagtactgca
     4021 gataccaaac ctgccgtcag catcccgtgg gctatcctcc ccccgaattt ggtgttcttc
     4081 atagcttcat gattaatatg cgccgggttg atatcccctg ttactcctgc atacatatat
     4141 atgtcagttt cgcttattgt cttctcgaag cttcccttat cccccacatt tatctgctcg
     4201 tacgtcttgc ccatttcatc tcctccttaa taaatgataa ctgtgtgctt taatctctat
     4261 atttaaatat atcacaggtt tgcactgttt caaatatgat tcagcaaatt cttcatgaaa
     4321 gcttcttgaa tttcaactcc ttttcatccg cgtcaatcag tatgtcattc ccgtcattaa
     4381 tctcccctgc aatcaaagcc cttcctattg aagtctccat tttcctctgt atgaaccttt
     4441 ttaaaggcct tgctccgtat accgggtcat aggaattgcc gattatatag tccttcgcat
     4501 tttcgcttaa tgagattttt atattcctgt ccttaagcct ttccgccacc tgttcaaata
     4561 tcaggtcaat gatttttctt atctgttcct tcagcagggg cttgaaaaac accacatcat
     4621 ccaccctgtt aaggaactca ggcctgaaat gccgtttaag ctcctcatta acggcctcta
     4681 ctgcctcggg cttcagctca ccgtcattgt ccaccccttc caaaagatac atgcttccta
     4741 tgtttgaagt cattattatc actgtgttct tgaaatccac agtcctgccc tgactgtccg
     4801 taagccttcc ttcgtcaagt atctgcagga atatgttaaa tacatcatga tgggcctttt
     4861 ctatttcatc aaacagcagt accgaataag gcttccttct gactgcctcg gtaagctgcc
     4921 ctccctcatc atagcccaca tatcccggag gtgctccaat cagccttgca accgtatgtt
     4981 tctccatgta ttccgacata tctatcctga taatgttgtc ctcactgtca aacagcgctt
     5041 ccgcaagggt cttggcaagt tctgtcttcc ctacccctgt aggcccgagg aatatgaagg
     5101 aaccgatagg cctcttcgga tccttgagcc cgcttcttgc acggatcacc gaatcggcta
     5161 cggcagatac tgcctcgtcc tggccgataa cccttttgtg gagtatgtca tccaaccgga
     5221 gaagtttttc tttttcattt tccattagtt tctttacagg tatacctgtc cattttgata
     5281 ttatatctga tatttcctct tctgtaacct cttccttgag aagctgcccc atgccctgca
     5341 ttttttcaag gtttgccttt tcagcattta actgcttctc aagctcaata agcgtgccgt
     5401 attttaattc cgcaaccctg ttgaggtcgt atttcatttc cgccttctca atttcagcct
     5461 ttgtctcctc aatcctcttc tttatctcgc ggatattctt aatgttctcc ttttccaatt
     5521 cccactgagc cttcatggaa tcgcctttgg acttcagctc tgaaagctcc ctgttcaagg
     5581 cctccaatct ttcctcggat tgcttgtctc tttctttttt cagtgcttcc ctttcaatct
     5641 ccagctgcat tatccttctc attatctcat caagctcacc tggcatgctg tcaatctccg
     5701 tacgcagcat ggctgcggct tcatcaataa ggtcaatggc cttgtccggc aggaacctgt
     5761 cactgatata tctgtttgaa agtactgcag ccgatatcag agcattatcc tgtatcctca
     5821 ccccgtgatg tatctcgaac ttctccttaa gccctctcag tattgatatg gtatcctcca
     5881 cgtccggagc gtctatgagc accggctgga atctcctttc aagagctgcg tccttttcga
     5941 tatgcttcct gtattcatcc agtgtagtag ccccgatgca gtgcagttcc ccccttgcaa
     6001 gcattggctt cagcaggttc cctgcatcca tggcaccttc cgccttccct gcgcccacta
     6061 tgttatggat ttcatctatg aacataatta ttctgccgtt ggatttttct atttctttaa
     6121 ggactgcctt gagtctctcc tcgaattccc ctctgtattt ggcaccggct ataagcgcac
     6181 ccatatccag ggagaaaatc gtcttgttct tcagcccctc aggcacatcc cccttcataa
     6241 tccgctgcgc caggccttct actgcagctg ttttcccaac tccgggctct ccgataagca
     6301 caggattgtt ctttgttctt ctggatagta tcctgattat tctccttatt tcattgtccc
     6361 tgccaatcac cgggtcaagc ttgcctttac gcgcatcttc caccaggtca cggccgaatt
     6421 ttttcagtgc ttcataagtt tcctccggat tttggccggt tatcctctga ttgcttctta
     6481 cggcggaaag ttcccttaag aacttttccc tggttattcc gtattttttt ataatttttg
     6541 aagaaggtgt atttgtttca tcaatcagcg ccagataaat atgttcaacg ctgatatatt
     6601 catcctggaa tttttttgct tcttcatccg cccttaagaa taattgctca aaccttctgg
     6661 aaacataagg actagaagct gtctctccgt aaatcttcgg aatcttgtca aggaggtctg
     6721 aaatatcctt ataataaaaa tcaaaaggca cgttcatttt tgaaagcagc ttgggaatca
     6781 atccttcatt ctgcaaaatg attgcgtaat gcaaatgctc tccttcaaca gtctgatgat
     6841 tgtgctttat tgccaggctc tgtgattcga taacagcctc ctgggctttt tgtgttaatt
     6901 tgttaatatc catgatgtta acctccgcgt tttgactttg actttctttg acttttaatt
     6961 attttatatc atatctttat ttcaattgca agctaaaatt ggaatcgtat caattatata
     7021 acactaatat tattctcgtt tcagtaaaac ataatatcag gaggtgatat aaatggctag
     7081 gaataatcag aaagtagtac ctcaggcaac ggcagcactt gataggatga aattcgaagt
     7141 agcatccgaa gtaggtgtta acttaaaaga aggatataat ggtgacattt cggcaaggga
     7201 cgcaggcaga gtaggcggga caatggtgaa gagattaatc gagcaggctg aaagaagcat
     7261 gtccggtaga taatatttaa aaaagggatg tcgcaaaact attgaataaa tagttttcga
     7321 tatccctttt tttagtcctt tcaattaatc aggtaagagc atcaaacgca cttttatggt
     7381 aaatcaaatc agtataaagg atattattgt acttctcgta acgtaccccc gcaaggctgt
     7441 atttctgctt tttcctgaac tggagggaag caaaattggg ctgagggctg attatggtct
     7501 ctgcaaaaat atgcttaatg ccttcattcc ttaagtcctc caggagcctc ttgtaaataa
     7561 ggcttccgat tcccataccc gtcaaatctg caaatacggc agttttgtca accacaataa
     7621 acttgttcgt ccagcttgga tcaaattccg gattccagta tacatcctgg agccagctgg
     7681 gattgtccct tagccactcc tcttttgtat aggctatcat gaatccgtat accttatcat
     7741 attcggcaac ataaaaacgc tccaggtttt caatccaatg gcctattttc tttttataat
     7801 aattaggttt cgactggtaa tcatccacaa gaaacccctg ttcggagatt ttctccgagg
     7861 ttcctaccga agatgcaaca tcgtatattg aattgatatc acttatttct gcttttctga
     7921 ctctaagaag ctttttgaga tgatacggat tgacaacact gtacgatttg gttgctactg
     7981 acatttcaat acctcctttt ttttaaagaa ggcgggtgat aacaggtaat ttgggggttg
     8041 aaaaagccgt ggagtaaacc acggctgcaa ctatttcaaa ttacacccag tcctcctctt
     8101 cccacgctta cgaggttagc tgacggattc ggactgaaag agtttagccc tacctgacta
     8161 ttcaggattc accccatttt ttggttcccc cgttcccttt gaaagggact cagcgatcga
     8221 ttgagcatat ttattttttt tgttacactc tattatattc cagcgggata aaaattacaa
     8281 caaaaatttt taatttttat atgaaaattt taaggtctgc cctccattac actaaatctt
     8341 tcttcaactc tcttcatcag gatatcataa ttcactatct gtctgacatg atagcccgta
     8401 ccgatccttc cgatctcatc aaaggcggta attttaaagg acaatacatt tccgttaatc
     8461 tcaacaagct cagcactcac agtaacagtt gtcccttctt tggttgcatg ctcatgctca
     8521 atctcaatgc tcttcccgat tgtaatatag cgatcaggaa gcagagggtc aatagttttt
     8581 actgtcgctt caatcatcag cgcagaaagt gtcggcgttg ccagcagatt cttcaatgct
     8641 ccgctgccaa aattcaatgc agtgtcttct ctggttatga ccttttgaac agattttgac
     8701 attccgatgg aaaattccgg catcttcata ataacacccc ctttttttta ttataccata
     8761 aaattacttc cgatataacc ctttcagcta ttttcactgt aacccggcta aaaaacagaa
     8821 tcccgggaat tgatgtgcac cctgggattc tttatgcttc aaagtttagc tgaaacctcg
     8881 tatcaaattt tatatgctgt aggttgtcaa ttcctcacaa tagccgcatc tgtaatgaat
     8941 ttttccattg gctttagcca ggtggaactt tggggttaca tactcgtcaa aattgctgac
     9001 gcacctggga ttcttgcatt tgaacaatcc ctgcacttct tccggtataa tggtgttcat
     9061 ctttttgact atcttatcgt tttcaattat gtttacggtt atattggggt ctatcaatcc
     9121 cagcattgta aggtccacat ctatcttgtt ctcgattttt atgatatcct tcctgccaag
     9181 cttcctgctt ggtacattaa tcagaagcac caccgggaaa tctacctcat caagcttaag
     9241 cttctcaaag atttttattc cattgcccgc agctatatgg tctattacta taccgcaatt
     9301 aatgctattt acggttaaca tttacattca cccccaataa gagcgccatc agcgccattc
     9361 tcacatatat tccgaattcc gcctgtttaa agtatactgc cctcttatca tcatcaacat
     9421 catagcttat ctcgttaacc ctcggcagag gatgcattac cagcatatcc tccctggaat
     9481 ccttaagctt gcttctgctt agaatataac tgtccttaag cctcatgtaa tccgcttcgt
     9541 taaagaacct ttctttttga attctcgtca tatacagcac atcaacctgg tttatcactt
     9601 cttcaaggtt ttctgtctca taaataccca cattatgctt ttcaataaga cttgacttca
     9661 agtgtccggg aagcctaagc tcagcaggag atataagtac gaattttgat cctttatagt
     9721 acgacattgc gcttatcagt gaatgaaccg tccttccgaa tttgagatcc ccgcagaaag
     9781 ctattgtctg attctccaat gttcctctat atcttcttat ggtaagcagg tccgtcaagg
     9841 tctgtgtcgg gtgctgatgc cctccgtcgc ctccgtttat tacaggaatt agtgaatatt
     9901 ccgaagcaag cttaggggct ccctccttgg ggtgcctcat taccacaaca tctgcgtatg
     9961 aactcaatac ccttatggtg tcagctatgc tttccccctt tgataccgag cttgtgcctg
    10021 catcagaaaa accaagtacc tttcctccca ttctcagcat tgccgattca aagctcagcc
    10081 ttgttcttgt acttggttca taaaacagtg tcccgagaat tttcccattg cagatattcc
    10141 caaatgaatc tctgtcatcc gccattctgt cagccaggtc aaaaatctca tccagctccg
    10201 acaggctgaa atcattgggg tcaatcaaat gtctgccttt taaactcatg tattctcctc
    10261 cttcttagtc tctccggact attatttaaa ggttacccat tcagggcata aaaaaaacct
    10321 cacacctgtg ggcatgaagt tattccagtc accagataca ctatcaaaaa ccttaccagc
    10381 ctcacaggac taaattaaag gttatctttt tttttaaaga tatcataaga cagaattgtt
    10441 gtcaataaaa tgatttgata agtttttttc aaattacacc caaaaatcat gaccaacctg
    10501 cgaattgtat attaatcaca aaaatccatt aaaagaaaaa agaaacaagg aggaaatctt
    10561 atgttgaaga aaaagttggc ggcactggga attgcagtag tattcacact ctcttcctta
    10621 actactacgg ttgcagcaga ttataaaaag ccggcagcag gaggaaatat aacaatcaaa
    10681 gttgtatctc ctggcaaagg taatgcatat gggcaaacca aaatcaagaa gaacgaagtg
    10741 aaaaaataca gatttgatga ttgcgctgat atcacctggg ccctcaatgc aattgaaaag
    10801 ctggccggaa aaggaataat cggaggcata ggcaacggca agtatgcgcc gaggagcaat
    10861 gtcactcatg tagaggctct tgcaatggtt atgagactga caggtgatga caaagaagcg
    10921 gaaggcatgg gaaaaaaggt tcatcccttc tataaaggta tattgcctaa ttggggctat
    10981 ggctatatat tcatggcaat agaaaaagga atcctgctcc ctgaagaact gaaggatttc
    11041 aatcccaacc aacctgcaaa gcgtcatgaa attgcaaagt atatcattag agcaatggga
    11101 gaaaccaaag aagctttaaa gcatatggat gaagaccttg atttcaagga cagcagcgct
    11161 gtaccggata aatcagtggg ttatgtgtac cttgtaagta aactgaacat tatgattggc
    11221 aatgaaaaaa atatgttcaa gccaatggag cctgtgacaa gggctgaaat ggccgtactt
    11281 ctcgacaggg ctgaaggcaa gttcgacctt ccggacactg acaacagaaa gaacagcaca
    11341 gtatttgtct ctgcagatgt tgatgacaat gaaatcacag tcaaggtaaa aggagtcact
    11401 gtaacttacg atgtgcttga agatgtacag gtgtacaggg atggttcctt tgaaactata
    11461 gaagacctgg taaaaggaga tattctgcag cttacgctga acaacagcaa agatgtaata
    11521 ttcattgagg ttgtaaagag tgctgaagaa ggcgacgatg atgaggaaga ggaaattgca
    11581 gtctccttcc gtgctgtatc tgacagcagc cttccaaaag tacttaagga tgaaattgac
    11641 gacatgaagt ctaaggaaag ctatgcagca tatgaatatg ataactatat ttaccttgta
    11701 gctgcaatgg gcaggcagag aaccggcggt tatgatatag aaatagaaaa ggtatatagg
    11761 attggggata acgatgaata tacaataaga gcggtagttg atacggatga accgtcatcc
    11821 ggctcaaatg tcacccaggc aatcacttac ccgtacagtg tagtaaaatt caagagcttt
    11881 gatgacattg aagaagtgat tttcgtagat gaagatgatg atgaacttga tgatgtagaa
    11941 attgaagacc ttgatgaaga ccctgaagaa accaaagtgt cttttgccga agttgattac
    12001 gatgatttgt cagccgtatt aaaggatcag gttgactatc tgaagctcaa taaaaacttc
    12061 aaggcttatg aatatgacaa cattatctac ctgatagcaa caatgggtaa aaagaactca
    12121 gaggactaca agattagtat agatgatgta tacaaggttg aaaacggaga caaatataca
    12181 ataaaagctg tagtagatac aactgtccct gcatcttcaa gcggcagcaa atcggtatac
    12241 ccgtacagca ttgtaaagtt aaggaatttc gatggtattg acggagtcag atttgttgac
    12301 gagggcaaca acaagctgtc cgaaacaaaa atagtagaat tggatgaggt tgttaccgca
    12361 gaagggataa tcagcgcaat taatacaagc ggcaaacaaa taagagtaaa gaagtcaaac
    12421 ggcacaacag ttgccctcac aataccaagc agtgctgaaa taacagtaaa cgatgatgac
    12481 gatgcaagca tttcggattt tgaagtgggc atgaaagtga aagtagatat tgtcggcgat
    12541 atagtaacag aagttattgc agaagatgat aaaacagaag taaccggcac actcacggga
    12601 ataagcatca gcactgtaaa gaaaatcact gtaaaagttg ataacacaac caaaacctac
    12661 acagtggatt cagatgtcaa ggtaataatt gatgatgaaa ctgaaagggt tgaagacctt
    12721 agagtaaacg atgaattgac cataaaattc attaacggaa ttctggcaga aatagaaaag
    12781 aaataaagca aatcttggtc atatccactc tctcggcagc caaaaccggc tgcctttttt
    12841 ttgttaatat tgtacttgcc cttgtaatat ttatttatta tagggttttt ataaggagtg
    12901 gataagtatt gaaaaagctc acatacatac aaggggcatt tattctgaca ataacaaacc
    12961 tcattaccgg atccctgtcc tttatataca gggtatttct taccagggca ataggctctg
    13021 aaggtgtagg ggtatatcag ctggtccttc ctttatacct gctttttata actttggttt
    13081 cgggaggaat cactacggca gtatcaaagc tgatcgctga acaggctccc ggcagcaatt
    13141 acaggaatat gtacaagatt attaaaatct gcgggatcac aatagggata tggagcttgg
    13201 ctttatgctc ctttatattc tttaactctg aatatctggc caaatatgtc ctcaaggaca
    13261 acaggacctt cttctcaatc atggtattta ctcctgccat attcttcata tccttaagtg
    13321 ccgtgttaaa aggatatttt tacgggctgc aatatgtcaa gcctccggcc atgattgaca
    13381 tacttgaaaa aatgatccgg ctgggagggc ttcttatgac tacatattat ctgcttccat
    13441 tgggaataga atacgtttgc gccggggtaa tggcagcgat ggctgcagga gagtttatca
    13501 gcctcattct tttgtacttc tgctacaaag tcaaaaaagc agctgtagtt catacaaaga
    13561 ctgattcaac tatccatatc ataaccagaa tactgaaaac agctcttccc ctgtccctaa
    13621 gcggttccct gacaacaatc atggatatga taacagccat attgataccc atgcagcttc
    13681 gtgcagcagg ctatagcaac tcaagtgcat tatcaatata cggccaggta acaggtatgg
    13741 ttatgcccct gcttttcttc cccttcatta ttgttgcttc ccttggcatt actcttgtcc
    13801 ctgccatagc cgcctcctat gccgcaggta attggacggc ccttaacaag aaatgcaatg
    13861 actccctcaa aatcacttca gcaatcgcat ttgcttcaac tgccctattt attgtattcc
    13921 cctgggagct atgcaggata ttcttcaaat gccccgaggc cggcaatatg cttttttgga
    13981 tgtctttcgt ttgtgtcttt gaatattggc agttcacttt atttgctgtt ttgaacggcg
    14041 tcgggaagca gcatgtggtt ctgaaaaact caatcctgca tattgttata tccgtcatat
    14101 gctcatattt ccttataccg ataccttcta taggaattta tggatttgta atcggattct
    14161 gtgcaagtgc agtgttgatt tgcctgtgga atctcgcaga cttaaagcgt attcccaata
    14221 tacatataga ttatgtcaat atatttatga agcccctttt cgcttttgtt ggaatgctca
    14281 tcgttatcaa gtgctgcaat ctgtatctgg tggcaaggac tttacaaaac ttcaacatga
    14341 cagtatcaag cattacaggc cttgtgttct tctttttgat actcttcttc atgggaattt
    14401 ttactcccaa acaactgagg aacacctttg caatgaaata aaaaaggagg gacatgggga
    14461 cgtttctcc
//
LOCUS       DAOQUR010000057        13717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5044_length_13717_cov_12.764529, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000057 DAOQUR010000000
VERSION     DAOQUR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13717
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5044_length_13717_cov_12.764529"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKI50_10080"
     CDS             complement(<1..194)
                     /locus_tag="PKI50_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT04089.1"
                     /translation="MIHTEIAIVGGGPAGLCAAIKAAESGAKVILLDRDSCLGGQLIK
                     QTHMFFGSQKQYASHRGIDIA"
     gene            complement(191..496)
                     /locus_tag="PKI50_10085"
     CDS             complement(191..496)
                     /locus_tag="PKI50_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT04090.1"
                     /translation="MRIEQHPIITFNKGRKVQFTFDGKVYEGYEGEPIAAALHDAGIR
                     VLGHTHKLGRPRGFYCAIGNCSSCLMVVDGKPNVRVCVEKLKEGMAVETQIGRGDIK"
     gene            complement(683..2095)
                     /locus_tag="PKI50_10090"
     CDS             complement(683..2095)
                     /locus_tag="PKI50_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT04091.1"
                     /translation="MPDNNEIKEFDIFDVEDGELLKIILDSINDAICVVDENCITRYW
                     NNAAEKLYNIGKDKILGRHIKEFFPNSLLPKIIKERKSYDNIYNNPREGCYNVISASP
                     LYMNGRLVGGISLDRDATEFIKTSELLMKAQSNLNVLEHEMSIINKNRYSFSQILGNN
                     RAFRDSIRLARDISRSNINVLITGESGTGKELFARAIHMESGREGYFVPINCSAIPHE
                     LIESELFGYTAGAFTGALKTGKIGKIELAHNGTLFLDEIGDMPLSMQPKILRVLENGE
                     MTRIGSEKTIKTNIRIITATNKNLSEMVEAGLFRKDLYYRLNSVVINLPPLRERKDDI
                     PLLVNRFIEDYCMEYGINILGITPEVLDSFVNHSWEGNIRELKNAVERIVVLARNSNT
                     GVIDTSFLPQSLGGIRKTISAVKQDSIYDLNTILENAEREALINVMRISGGNKAHGAK
                     LLNIPRSTLYFKLKKHNLEK"
     misc_binding    2236..2450
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2509..3954
                     /gene="proS"
                     /locus_tag="PKI50_10095"
     CDS             2509..3954
                     /gene="proS"
                     /locus_tag="PKI50_10095"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNT04092.1"
                     /translation="MADKKQQEFVKEITPMEEDFSQWYTDVILKTDMVDYAPVKGCMV
                     IKPYGYGVWEGIQRVADRRFKATGHKNAYFPLLIPESYLKKEADHFEGFAPEVLWVTH
                     GGSEELTERLFIRPTSETIICDMYSRWVQSYRDLPLLINQWCNVVRWEKTTRPFLRTA
                     EFLWQEGHTVHADEEDAQRETLLILEIYRQVAEDELAMPVILGQKSEKEKFAGALRTY
                     TMEAMMHDGKALQAGTSHNLAQHFSKMFDIKFLDKDGVLKYAHSTSWGISTRLIGGII
                     MVHGDNRGLVMPPRVAPVQVVIIPIAFHKEGVLDKAKAVKDELEKCFRVEMDDRDTYS
                     PGWKFNEWEMKGVPIRMEIGPRDIENNQVTIARRDTLEKFTVSMDNLTETVSGLLDEI
                     HTTMFNKALKNMEEKTYTTTDYEEFKKIMQETPGFVKTMWCGSRECEDKLKEDTAATI
                     RCIPFRQEHVGDKCLICGGKAEKMVYAAKAY"
     gene            complement(4079..4291)
                     /locus_tag="PKI50_10100"
     CDS             complement(4079..4291)
                     /locus_tag="PKI50_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04093.1"
                     /translation="MAKLDMIGQIADLKEIDYKNTLAITSIIELLIEKGILDSSEVAA
                     KAQYLEDQTLSEIRRNSRFGIRTDFR"
     misc_binding    4436..4636
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4690..5964
                     /gene="serS"
                     /locus_tag="PKI50_10105"
     CDS             4690..5964
                     /gene="serS"
                     /locus_tag="PKI50_10105"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNT04094.1"
                     /translation="MLDIRAIRSNPEEVKRKLTRRGQEFRIDEVLKLDEQRRQVIYEV
                     EELKSRKNKVSEEVAKLKRAKQDAEAVVLEMRQVSEKIKEMDERVRNLDEEITGILLT
                     IPNTPYDGIPDGSSDADNVEIRKQGEPTKFEYEPKAHWDIGVDLNILDFETAGKVTGA
                     RFTFYRGLGAKLERALINFMLDTHTENGYTEIFPPFMVHRRSMTGTGQLPKFEEDAFK
                     VQNTEYFLVPTAEVPVTNMYREQILDGSMLPIRHTAYTACFRAEAGSAGRDTRGLIRQ
                     HQFNKVELVKFTNPENSYEELEKLTEDAERILKLLKLPYRVVCLCAGDLGFSSAKTYD
                     LEVWMPSYGRYVEISSCSNFEDFQARRADIKFRPEPKEKPRFVHTLNGSGLAVGRTVA
                     AILENFQQGDGSVIIPEVLRPYMGGLDKITKA"
     gene            6083..>7305
                     /locus_tag="PKI50_10110"
     CDS             6083..>7305
                     /locus_tag="PKI50_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT04095.1"
                     /translation="MSGKEVKKHKHSTLSGYIVQTLLMVTPLAGIAGTLMSIYIVRLE
                     GLQLKLSVACYLLAGIMLGIMASFRNYSKFIKPLNEISNLADTLKNSNITYRIDTSKA
                     GGQLEIIESLNESVSNLRSTVKDAMEVGSQVSAAPLEMKKSIQILSSISEQAAAALSE
                     LAKGAVEQAGSIEEISNSVSGIVSGLSKITTDMDDTDKLAEKAIDTIKLGENSVNYQE
                     VKINENKEFVTSVSSAVSALAEKSKEIGDILLVINGIAEQTNMLSLNAAIEAARAGEQ
                     GKGFAVVANEVRKLAEQSGNSVQKIEELIREVQSGIEKAVQEMTKVESIADEQQKAMS
                     ATVKSFAGIFEAVSEMSANIREVNAESVNITKNASQAETAIGQIAKISEEAAAATEQV
                     SASTDEQANAVRSIL"
     assembly_gap    7306..7405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7666..7755
                     /locus_tag="PKI50_10115"
     tRNA            7666..7755
                     /locus_tag="PKI50_10115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7700..7702,aa:Ser,seq:tga)
     gene            8120..8212
                     /locus_tag="PKI50_10120"
     tRNA            8120..8212
                     /locus_tag="PKI50_10120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8156..8158,aa:Ser,seq:gct)
     gene            complement(8294..9490)
                     /locus_tag="PKI50_10125"
     CDS             complement(8294..9490)
                     /locus_tag="PKI50_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT04096.1"
                     /translation="MKEKGMMTRLTHAGENAIAKKAAASVSVPKVIPIYMSSVFSFDD
                     VDSLDAVYAGTAQGYVYSRMTNPGTDCVEEVLAQAEDCGGALVFSSGMAAIVTTIISQ
                     VKAGDHIISSPVLYGGVYDYLKNELPRFGVEVTFADFEKDDIESLIRSNTKILYTETI
                     SNPLMAVEDLPSISKIAHSHGCKLIVDNTFATPAIAQPLKLGADISVYSATKYLGGHS
                     DIIGGAVMSDAENIAHIHHFLTLYGAIMGPFDAWLLTRSLRTLDMRMERHSANALKVA
                     EFFEKHSKIERVYYPGLASSPYKKRTQESFTYGRCGGMLSADIAGGEKGACTFIRECK
                     NIKFVPSLAGVATSISYPAKTSHRAYSPQELKNVGISMGLLRFSVGLENIDDVLEEFD
                     AALKKV"
     gene            9669..10547
                     /locus_tag="PKI50_10130"
     CDS             9669..10547
                     /locus_tag="PKI50_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNT04097.1"
                     /translation="MKKLKFIYNPYSGEGVFKDRLDYLIERFQQAGYMMVPFRSTNIN
                     TLDRAFFDIHEDYEAVCISGGDGTLSTVTNIMASRKLKLPVGIFPFGTANDFATHLNI
                     PGDIEACCEIIEKGRTKKVDIGRVNNSYFLNVCSAGLLSDVAYETDTNLKNALGKLAY
                     YMKGIEKIPRFTPFKMRLQYGSNVIEDNFLLLLILNGRSAGGFSRLAPNAVIDDGLLD
                     VIAVKNTNITNILALFLKILRGEHIGDTNLYNFQADRLAITCEGGCETDIDGERGPDF
                     PLDIEVKKHFLKVFVP"
     gene            complement(10660..11517)
                     /locus_tag="PKI50_10135"
     CDS             complement(10660..11517)
                     /locus_tag="PKI50_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT04098.1"
                     /translation="MIEYRKLAHEDYDDILDICKDIWDGTDYLPEIFHKWVDDKGLFL
                     GAVDTDTNKVIGTDKYSVLYDGSGWLEGLRTHKDYRGRGIGKGLAVRTFNKALEDLGS
                     GRINKIAFSTSALSVESIGLMTKLGFRLKQEYKMVWKDYSDADNSLSIGDFAAESWKP
                     SYDEFANQPYIKRRDGILPFVFLFQKPTPELYEELLKEKCFVSINGHKGLFKLKGDPH
                     FIVFDESFEGINTFMNYYLLALAGKCPVPPLVSVMPADTELIEALNAAGFGMMDPSTC
                     DYLYYIYEG"
     gene            11714..12799
                     /locus_tag="PKI50_10140"
     CDS             11714..12799
                     /locus_tag="PKI50_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNT04099.1"
                     /translation="MEAEKYKVAETAVEEADLSKLTEEQKQRLEEIIKTINLEDSQFI
                     LQYGVGAQSQIAGFADNIVSEVRAKDSGYVGDILTDLMLKVKELDVDGVSSGKGFMSR
                     FPIFGSLVNNTQKFAARYQKLSTEIEKIVDELDKSKMQLLKDITLLDYLFEKNLEYLG
                     NLNYYIMAGTLKLKEINEKIIPEMQSKVNETNDPLDAQKLNDLIQMANRFEKKIHDLK
                     LSRMVSLQTAPQIRLIQNNNQVLVEKIQSSILNTIPLWKNQIVIAISLAKQKNALAVQ
                     KEVTDTTNTLLQKNAEMLKTGSIDLARETERGVVDIETLKKVNEDLIATIDETLNIQK
                     EGRIKRKQAEEELAAIEKELKQKLIGK"
     gene            12830..>13717
                     /locus_tag="PKI50_10145"
     CDS             12830..>13717
                     /locus_tag="PKI50_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNT04100.1"
                     /translation="MNQRWSLDELYKSFESKEFKKDYEKCIKDIERIKEWAESNFKDS
                     SDAAHKVRRYIKTINEFYMLYSRLYSYAQLTVSVEARNEKALKVIDCLEEKATELTKP
                     EVSFQKWLGTISNINEIIASDSYLKKFEFFFRENLEKSKYLLSDKEEALISKMKTTGS
                     AAWEKLQELLTSTLLVEITLDGEDKKLPLPIIRNMAYNKDAELRKKAYEAELKAYQKI
                     DESVAACLNGIKGEVITVSRMKGYKSPLEKTILDSRMDLETLYAMIAAMRESLPDFHR
                     YFRKKAELMGYKNGLPFYEM"
BASE COUNT         4160 a         2745 c         3191 g         3521 t
ORIGIN      
        1 gcaatatcta tacctctgtg tgaggcatat tgtttctggg aaccaaaaaa catatgagtc
       61 tgttttatca actgcccgcc cagacagctg tctctatcaa gcagtataac cttcgcaccg
      121 gattcggctg cctttatagc ggcgcaaagt cccgcaggtc ctcctcctac tatagctatt
      181 tctgtatgaa tcacttaata tccccccgtc ctatttgagt ttccactgcc attccttctt
      241 taagcttttc cacacaaact ctcacattgg gcttgccatc caccaccata aggcaggagg
      301 aacaatttcc tatagcacag taaaagcctc tgggtcttcc cagcttgtgg gtatggccca
      361 gaacccttat tccggcatcg tggagagcag ctgcaatcgg ctcaccttca tatccctcat
      421 aaacctttcc gtcaaatgtg aactgaacct ttctgccttt gttgaacgta ataataggat
      481 gttgctcaat cctcataaag gattccccct ttcgctaatt aaaaaattat atagcaattt
      541 atatgccaac gcaggaaaca attcatttta ctaaggtcta tatatttatg tgtcaatata
      601 ttgacagttg gattggtttt agacacaata gatgccaata tattgacgcg catgtcgaag
      661 tattgacgtt ttcgggagaa tgtcattttt ccaggttatg ctttttaagc ttgaaataca
      721 aggtgcttct cggtatattc aggagctttg ccccatgtgc cttattgcct cccgaaatcc
      781 tcatgacatt tatcaaggct tctctttctg cgttttccag tatggtattc aagtcatata
      841 tactgtcctg cttcacggca gagatggttt ttctgattcc tccgaggctt tggggaagaa
      901 aagaagtatc aattacccct gtgttggaat tcctcgccag caccacgatt ctttcgacgg
      961 cgtttttcaa ttctcttatg tttccttccc agctgtgatt tacaaagctg tccaaaacct
     1021 caggagtaat gccaaggata ttgatcccgt attccatgca gtaatcttct ataaatctgt
     1081 ttacaagcaa tggtatatca tccttccttt ctcttaaagg aggaaggttt ataacaacgg
     1141 agttaagcct gtaataaagg tctttcctga acaatcctgc ctccaccatt tccgataagt
     1201 tcttgttggt ggcagtgatg attcttatgt ttgtttttat ggttttttcg ctgcctattc
     1261 tggtcatctc tccattttca agcaccctga gtatttttgg ctgcatactc agcggcatgt
     1321 cccctatctc atcaagaaaa agcgtaccgt tatgtgccag ctcaattttg cctatcttgc
     1381 ctgttttcag tgccccggtg aaggctcccg ctgtatatcc gaataattcg ctttcaatta
     1441 attcgtgagg tatggcactg cagttgatgg gtacaaaata accttcccgt ccgctttcca
     1501 tatgtattgc acgggcgaac agttccttgc cggtaccgct ctcaccggtt atcagcacgt
     1561 tgatattgga ccttgaaata tctcttgcca gtctgatgct gtctctgaaa gctctgttgt
     1621 tgcccagaat ctgtgaaaat gagtatcgat ttttattgat tatggacatt tcatgttcca
     1681 atacattaag attggattgg gccttcatca gcagctctga ggtctttatg aactctgtgg
     1741 catcccgatc caggcttatg cctccgacaa ggcggccatt catgtatagc ggggaagcgc
     1801 ttatgacatt gtaacaccct tctctgggat tgttgtaaat attgtcataa gattttcttt
     1861 ccttaattat cttagggagc agggagtttg ggaaaaattc ctttatatgc ctgcccaata
     1921 tcttatcctt gccgatattg taaagctttt ctgcggcatt gttccagtag cgggttatac
     1981 agttctcgtc taccacacaa atagcgtcgt tgatactgtc aagaattatc tttaaaagtt
     2041 ctccgtcttc tacgtcaaat atatcaaatt cctttatttc attattatcc ggcatatcaa
     2101 cacttcctcg caaagatttt caaccttcaa tatatatact actattttca ccctttacat
     2161 tcaactgtat aatagcagaa tgatgtgtat attgacaggt caaatgccat aatatataat
     2221 aagcttaatt caaaacctaa ggcaatgaag aggaatagta gataacaagt cgccttaaga
     2281 gaggaaggct taagagctga aatgctttcc aggttagatt ttatcgaacc caacctccga
     2341 gccggggatg aactcccgcc gtatgcctgc gttaaaggct gaagaggaat atacgtataa
     2401 cggcgtatgt tcaaataggg tggtaccgcg agtaaaactt cgtccctttc atgtgacgaa
     2461 gtttttttat ttgttaatat caatttatta agataaggag agattattat ggctgacaag
     2521 aagcagcagg aatttgttaa agagattacc ccgatggaag aggatttttc ccagtggtat
     2581 actgatgtca tcttaaagac cgatatggtt gattatgctc ccgtaaaagg ctgcatggta
     2641 ataaagcctt acggctatgg agtatgggaa ggaattcaga gagtagccga cagaaggttc
     2701 aaggctacag gtcacaaaaa cgcatatttc ccgcttttga taccggaaag ctatctgaaa
     2761 aaggaagctg accactttga aggatttgct cccgaggtgc tgtgggttac ccacgggggc
     2821 agcgaggagc ttaccgagag attgtttata aggccgacct cggaaactat aatatgcgat
     2881 atgtattcca gatgggtgca atcatacagg gatcttcccc tgttgataaa ccagtggtgc
     2941 aacgttgtgc ggtgggaaaa gactacaagg cccttcctca ggacggcaga gttcctatgg
     3001 caggaaggcc atacggtaca tgcagatgaa gaggatgccc agagggaaac cttgctgata
     3061 ctggaaatat acaggcaggt tgcggaagac gaactggcaa tgcctgtgat acttgggcaa
     3121 aagagtgaaa aggaaaaatt cgcaggggct ctccgcactt atacgatgga agcgatgatg
     3181 catgacggaa aagctctgca ggcgggtaca tcacataatc tggcgcagca tttttcaaag
     3241 atgtttgata taaaattcct ggataaggat ggcgtgctta agtatgccca cagcacctcc
     3301 tgggggattt ccacaaggct tatcggggga atcataatgg tccatgggga caacagagga
     3361 cttgtaatgc ctccgagagt ggctccggta caggttgtca ttattcctat agctttccat
     3421 aaggaaggag tgctggataa ggcaaaggca gtgaaagatg aactggaaaa gtgcttcagg
     3481 gtcgaaatgg acgacaggga tacctacagc cccggctgga agttcaacga gtgggagatg
     3541 aagggtgttc cgataagaat ggagataggc cccagagaca tagaaaacaa ccaggttaca
     3601 atagccagaa gggatacttt ggagaaattc accgtatcca tggacaatct gacagagacc
     3661 gtctccgggc ttctggatga gattcatacc acaatgttca acaaagcgct gaagaatatg
     3721 gaagaaaaga cctataccac tacggattat gaggaattca aaaaaataat gcaggaaacg
     3781 ccgggcttcg taaagacaat gtggtgcggc agcagggaat gcgaagataa gctcaaggag
     3841 gacaccgccg ctacaataag gtgcataccc ttccggcagg agcatgttgg agataaatgc
     3901 ctgatttgcg gaggcaaggc ggaaaaaatg gtttatgcgg caaaagcata ttaatttatt
     3961 aatggcatgt atatcggctt attgatatac atgccattta atactaatgc tttagcgttt
     4021 caacgaagca ggggacgtgc ttaccccctg tgggacatct tttgcctgat tatatttatc
     4081 agcggaagtc ggttcttatc ccgaatctgc tgttccttct gatttcggac agagtctggt
     4141 cttcaagata ctgtgccttt gcggctactt cgctgctgtc aaggatgccc ttttctatca
     4201 gaagctcaat aatacttgtt attgccagtg tgtttttgta gtctatttcc tttaaatctg
     4261 caatttgacc gatcatatcc agctttgcca taagagtacc tccccggtta ttatatgttt
     4321 tggccaagtt ctattaagag tattgcctgg tgcaggcata ttatacattt tatagaagcg
     4381 gatattgcaa aaatgcataa ttagtagtag aatagtttaa aatatgtata atacataaaa
     4441 tgctttgaag ggaaaagcta taggtgcgcc atcaagagag cgggcggcag gtgcaagccg
     4501 gctggcagct taaagccatc catcccggag cagtctgcga ggagcagtac cggtacagcc
     4561 gttaaacata tgagagagca gtgaaattgg ttactgctaa ttagggtggc aacgcggaaa
     4621 gcctttcgtc ccttatcatg gggaaggaag gcttttatat tttctaatca agaaaaggag
     4681 gaaaacacta tgctggatat cagagcaatc agatccaatc ctgaggaggt aaaaagaaag
     4741 ctcacaagaa gaggacagga gttcaggatt gatgaggttc tgaagcttga tgaacaaaga
     4801 agacaggtta tttatgaggt agaagagctt aaaagcagaa aaaacaaagt ttccgaagag
     4861 gttgcaaagc ttaagagagc gaagcaggat gccgaggctg tagttttgga aatgaggcag
     4921 gtatctgaaa agataaagga aatggatgag agggtaagaa acctggatga ggaaataacg
     4981 ggcattctcc ttaccatacc caatactcct tatgacggca tacctgacgg cagctccgat
     5041 gcggataatg tggaaatccg gaagcaggga gaaccgacta agtttgaata tgagccaaag
     5101 gctcattggg atatcggcgt cgatctcaac atactggact ttgaaaccgc aggaaaggtg
     5161 acgggagcaa ggttcacctt ttacagaggg cttggagcaa agctggagag agcattgata
     5221 aacttcatgc tggatactca caccgaaaac ggctatacgg aaatattccc tccttttatg
     5281 gttcacagga gaagcatgac gggaacaggc cagctgccga agtttgaaga ggatgccttt
     5341 aaggttcaga atacggaata tttccttgta cccactgcag aagtacctgt taccaatatg
     5401 tacagggagc agattttaga cggcagcatg ctgcctataa ggcatacggc atataccgcc
     5461 tgcttcaggg ctgaagcggg ttcggcagga agggatacaa gaggacttat aagacagcat
     5521 cagttcaaca aggttgagct tgtgaaattt acaaatcctg aaaattctta tgaagaactg
     5581 gagaagctga ctgaggatgc ggaaaggata ttgaagctct taaagcttcc ttacagggtg
     5641 gtttgcctgt gtgccggaga tttaggcttt tcttctgcaa agacctatga tttggaggtt
     5701 tggatgccga gctacggaag gtatgtagaa atatcctcct gcagcaattt tgaagatttc
     5761 caggcaagaa gggcagatat taaattcagg cccgaaccca aggaaaaacc cagatttgtt
     5821 catactctca acggctcagg cttggctgtg ggaagaacag tagcggccat acttgaaaac
     5881 ttccagcagg gtgacggaag cgttataata cctgaggttt tgaggcctta tatgggaggt
     5941 ctggacaaaa taacaaaggc ataagaaatt tttgaaaagc gtgtcaattc gacacgtttt
     6001 tcattgctca ttgtaatgcc tgtaaaaatt tgataaaatt gtaataggca tttgtcaaat
     6061 attatgaggg ggtgattcaa aaatgtccgg aaaagaagta aaaaagcata aacattctac
     6121 tttatcaggg tatatagttc aaacactcct tatggtgaca ccgcttgccg gaatagccgg
     6181 cacgctgatg tccatttata tcgtcaggct ggaaggcctt cagcttaaat tgtccgtggc
     6241 ttgctacctg ttagccggaa taatgttagg tattatggca tccttcagga attattcgaa
     6301 gtttataaag ccgctgaatg aaataagcaa tctggcagat acccttaaga acagcaacat
     6361 aacataccgg attgacacga gcaaagcagg agggcaatta gaaataatag aaagcctaaa
     6421 tgaatcggta tcaaatctga gaagcacagt taaagatgca atggaagtag gttcgcaggt
     6481 atctgcagcc cctttggaaa tgaagaaatc gattcaaata ctgagcagta tatccgaaca
     6541 ggcagcagct gcgcttagtg agcttgccaa gggtgctgtt gagcaagcag gttcaataga
     6601 agaaataagc aattctgtca gcggtatagt aagcggatta agcaaaataa ctaccgacat
     6661 ggatgatacg gataagctgg cggagaaagc cattgatacc attaagctgg gagaaaattc
     6721 agtaaattat caggaagtaa aaatcaatga gaacaaagaa tttgtcacaa gcgtgagcag
     6781 tgccgtttcg gcactggcag agaagtctaa agaaatcggg gatatattat tggtaatcaa
     6841 cggtatagcc gagcagacaa atatgctctc tctcaatgca gccattgaag cagcaagagc
     6901 cggtgaacag ggaaagggct ttgcagtagt agcaaatgag gtaagaaagc ttgcagagca
     6961 atccggaaac tcagtgcaaa aaattgaaga attaatcaga gaagtacagt cgggtataga
     7021 aaaagcagtg caggaaatga ctaaagttga aagcattgca gatgaacagc aaaaagccat
     7081 gtccgctacg gtaaaatctt ttgccggaat atttgaagca gtttcggaaa tgtctgccaa
     7141 tataagagag gttaatgcag aatcagtaaa cataacaaaa aatgccagtc aagcagaaac
     7201 agcaatcgga cagattgcca agatatcaga agaagccgct gctgcaaccg aacaagtatc
     7261 tgcttcaact gatgaacagg caaatgcggt tcgaagcata ttggannnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnaacaa gtatctgctt caactgatga acaggcaaat
     7441 gcggttcgaa gcatattgga gtcatccgag aaactatccg agcttgcagg aaggttaaaa
     7501 gggagtataa acaatttcac ggtataagag catttaaata atgaaatgat aatagaattg
     7561 tctgtaccaa gtttatgatg aagtgtaata agcagactgt atatcataat atttgtttat
     7621 tgacaccaaa aattaatatt gctataatgt atattgttca cgttgggaga gatgttcgag
     7681 tggtttatgg agctggtctt gaaaaccaga ggcgtgttac agcgtccgag ggttcgaatc
     7741 cctctctctc ctccatttta ataattaatc agacagaata gaaagagagg gtgagaaccc
     7801 tcaaataaat ggccaataag gaaatattaa tcaataatta tcaatggcca cagacaatgg
     7861 agatttacgg acagagattg gcagccggcc atccggcttc gaatccctct ctctcctcca
     7921 ttttaataat taatcagaca gaatagaaag agagggtgag aaccctcaaa taaatggcca
     7981 ataaggaaat attaatcaat aattatcaat ggccacagac aatggagatt tacggacaga
     8041 gattggcagc cggccatccg gcttcgaatc cctctctctc ctccatttta attttataaa
     8101 aattttttaa gtagtccaag gagaagtact caagtttggt cgaagaggcg cccctgctaa
     8161 gggcgtaggt cgggaaactg gcgcgagggt tcgaatccct ccttctccgc cataaagaaa
     8221 ttaactgccg aaaggcagtt tatttgttta tgcagaagga gggtatgaga accctcaaag
     8281 aaaggcctaa aaatcaaacc ttcttcaaag ccgcgtcaaa ttcctccagc acgtcgtcaa
     8341 tattctcaag gccgacggag aatctcagta agcccattga gataccaacg tttttcagct
     8401 cctgaggaga atacgcacga tgagaagttt ttgccgggta ggagatggag gtggcaacac
     8461 cggcaaggct gggaacaaac ttgatgttct tacactcacg gatgaaagtg caagcaccct
     8521 tttcaccgcc ggcaatatca gcagacagca tacccccgca gcggccgtat gtaaaggatt
     8581 cctgagtccg tttcttatag ggggaggaag caaggccggg atagtagacc ctctcaattt
     8641 tggaatgctt ttcaaagaac tccgctacct tcaaggcgtt ggcagaatgg cgctccatgc
     8701 gcatgtccaa agtgcgcagg ctcctggtca gcagccaagc gtcgaagggc cccataatgg
     8761 caccgtacag ggtgaggaag tggtggatgt gagcgatgtt ctctgcatca gacatcacgg
     8821 cgccgccaat aatgtcgctg tggccgccta agtacttcgt cgcgctgtaa accgaaatat
     8881 ctgcgcccag tttcagcggc tgcgctatgg cgggagtagc aaaggtatta tctactatca
     8941 gcttgcagcc atgcgaatgc gcaattttgg agattgaggg gagatcctcc accgccatta
     9001 aaggattgga gatagtctcg gtgtaaagaa tctttgtatt ggaacgaatc agagattcaa
     9061 tatcgtcttt ttcaaaatca gcgaaggtta cctcgacgcc aaaacgaggc agttcatttt
     9121 tcagataatc atatacaccg ccatacagca caggagatga aataatatga tcgcctgctt
     9181 ttacctgaga aataatagtg gtgacgatgg cggccatacc ggaggaaaac actaaggcgc
     9241 cgccgcagtc ttccgcctgg gccaaaacct cttccacaca gtcagtgccg gggttcgtca
     9301 tacgggaata gacataaccc tgggcagtac cggcgtacac ggcatccagc gaatccacat
     9361 cgtcaaagga aaatacactg ctcatgtaga tcggaataac cttcggcact gagacagatg
     9421 cggcagcttt ctttgctatt gcattctcgc cggcatgggt tagtcgagtc atcattcctt
     9481 tttctttcat aatatttttc ctcctcataa atttacggct ttaggcttga atgccgcctc
     9541 atacaattat tatatcattt aaatggcgat taattgtcaa tgacattaca gcagctgcat
     9601 acatagaata aaaatgaaac gaattagtag aataaactat gatataatgt gtaaagtggg
     9661 gtgagagcat gaaaaagctg aaattcatat ataatcctta ctccggagaa ggtgttttca
     9721 aggacaggct tgattatctg attgaaaggt ttcaacaagc cgggtatatg atggttcctt
     9781 ttagaagcac aaatatcaat acacttgaca gggctttttt tgacatacat gaggattatg
     9841 aggctgtttg catttcagga ggcgacggta ctttaagcac tgttacaaat ataatggcgt
     9901 caagaaagct gaagcttccc gtcgggatat ttcccttcgg tacagccaac gattttgcaa
     9961 cacaccttaa tataccggga gatattgaag cctgctgtga aataatagaa aagggcagga
    10021 caaaaaaagt tgatatagga agggtcaaca acagctactt tttaaacgta tgcagtgcgg
    10081 ggctgctttc ggatgttgcc tatgaaacgg atacgaattt gaaaaatgcc ctgggaaaac
    10141 tggcttatta catgaaaggc atagagaaga tacccagatt tactccgttc aaaatgaggc
    10201 ttcagtatgg aagcaatgta atcgaagaca actttctgct gctcctcata ttgaacggca
    10261 gatctgcggg aggcttcagc aggctcgcac ccaatgcggt aatagatgac gggctgctgg
    10321 acgtaatagc ggttaaaaac acaaatataa caaatatact tgcgttattt ttgaaaatat
    10381 tgaggggaga gcatatagga gacaccaacc tctataattt tcaggccgac aggcttgcta
    10441 ttacctgtga aggcggctgt gaaaccgata ttgacggaga acgggggccg gacttccctt
    10501 tggatataga ggtcaagaag cattttttga aggtatttgt accgtaatac gggacacggg
    10561 ggacgtttcg ggacacgggg acgtttctcc tgtcccgctg aaagcgggac aggagaaacg
    10621 tccccgtgtc ccgaaacgtc cccgtgtcct cgcgtcacat tatccttcgt atatataata
    10681 cagataatcg caggttgaag ggtccatcat accgaatcct gcagcattga gggcctcaat
    10741 cagttccgta tctgccggca taaccgagac cagaggcgga acggggcatt tgcccgcaag
    10801 ggccagtaaa tagtaattca taaaggtatt tataccctca aagctttcat caaacactat
    10861 gaaatgcgga tctcctttta acttgaaaag ccccttgtgc ccgtttatcg atacgaagca
    10921 tttttccttc agcagctctt catacagctc cggagtgggt ttctgaaaaa ggaacacaaa
    10981 aggcaggatg ccgtctcttc ttttaatata gggttgattt gcaaactcgt cataggaagg
    11041 cttccagctt tctgcggcaa agtctccgat gctcaatgaa ttatctgcat cgctgtagtc
    11101 cttccaaacc attttatact cctgtttcag cctaaaaccg agttttgtca tcaaaccgat
    11161 gctttcaacc gagagagcac tggtggaaaa tgctatttta tttattcttc cgcttccaag
    11221 gtcctccaag gccttattaa aggtcctgac agccagcccc tttcctatgc cgcgcccccg
    11281 gtaatcctta tgggttctca agccttcaag ccagccgctg ccgtcgtaaa gcacagaata
    11341 cttgtctgtt cctattacct tgttggtatc ggtatcaacg gcaccaagaa acaagccctt
    11401 atcatcaacc catttatgga aaatctcagg gagataatcg gttccatccc agatatcctt
    11461 acagatatcc aatatatcat cataatcttc atgagcaagc tttctgtatt ctatcataca
    11521 attacccctt ctatatcgta attttttcta tattttccgg caaataggct atatacaatt
    11581 atactatacg catatatatc tttgcactga ttgcatatat atttttatga aaaaaatgat
    11641 aaaatggtat tgtatcgaga ttagtaaaag ctcaaatatt tacgtatata tattgtacga
    11701 aggaggaatg attatggaag cggaaaaata caaggttgcg gaaaccgcag tggaggaagc
    11761 tgacctttcc aagcttactg aagagcagaa acagaggctg gaagaaataa taaaaactat
    11821 taatctggag gattcccagt tcatacttca gtacggggtg ggggcccaaa gccagatagc
    11881 cggttttgcg gataacattg taagcgaggt aagagcaaag gacagcggtt atgtgggaga
    11941 tatactcacc gaccttatgc ttaaggtaaa agaactggat gttgatggtg taagctcggg
    12001 caagggattc atgtccaggt ttcccatttt cggatcttta gttaacaata cccagaagtt
    12061 tgccgcaaga tatcagaagc tcagcaccga gattgagaaa atagtagatg aactggacaa
    12121 atccaagatg cagctgttaa aggatatcac cctgctggat tacctgtttg agaaaaacct
    12181 tgaatacctg ggcaacctca attattacat aatggccgga actttgaagc ttaaggaaat
    12241 taacgaaaag ataatcccgg aaatgcagtc caaggtgaat gaaaccaatg accctttaga
    12301 tgcgcagaag ctgaatgacc ttatacagat ggcaaacaga tttgagaaga aaatacacga
    12361 cctgaagctc agcaggatgg tgtccctgca gacagcgccc caaataaggc ttattcagaa
    12421 caataaccag gtactggtgg agaagataca gagttctata cttaatacca tacccctgtg
    12481 gaaaaatcag atagttattg caataagcct ggcaaagcag aaaaatgccc tggcggtgca
    12541 gaaggaagta accgacacca ccaataccct gcttcagaag aatgcggaga tgctgaagac
    12601 cggttccatt gacctggcaa gggagactga gcggggagtt gtggacatag aaactcttaa
    12661 gaaggtaaat gaggatctta tagccacaat tgacgagacc ctgaatatcc agaaggaagg
    12721 ccgcatcaag aggaagcagg cggaagaaga gcttgcggct atagaaaaag agcttaaaca
    12781 aaaactgatc gggaaatgat atatataaaa attgagtgga ggttatccaa tgaatcagag
    12841 gtggagtctg gatgaactgt ataaatcctt tgagtcaaaa gagttcaaga aggactatga
    12901 aaagtgtatc aaggatattg agaggataaa ggaatgggct gagagcaatt ttaaggactc
    12961 atcagatgca gcccataagg tgagaagata tataaagacc ataaatgagt tctatatgct
    13021 gtattcacgg ctttacagct acgcacagct taccgtgagc gtggaagcca gaaatgaaaa
    13081 ggcactgaaa gtaatcgact gcctggagga aaaagccaca gagcttacaa agcccgaagt
    13141 aagcttccag aaatggcttg gaaccataag caatataaat gaaatcattg cctctgacag
    13201 ttacctgaag aagttcgagt ttttcttcag ggagaatctc gaaaagagca aatatctgct
    13261 aagcgacaaa gaagaagcgc tgatatcaaa aatgaagact acgggttcgg ctgcatggga
    13321 aaagcttcag gagcttttaa cctccacact tcttgtggaa attacattgg acggagagga
    13381 taagaagctt ccgctgccga taatcaggaa catggcatac aataaggatg ctgagcttag
    13441 aaagaaagcc tatgaagcgg agctgaaggc ttatcaaaag attgacgaat ctgtggccgc
    13501 ctgcttgaac ggtataaagg gagaagtaat taccgtatcg cggatgaagg ggtacaagtc
    13561 acctttggag aagacgattc tggattcaag gatggatttg gaaacactgt atgcaatgat
    13621 tgccgcaatg cgggaaagcc tgccggactt tcacaggtat ttcaggaaaa aagcagagct
    13681 tatgggatat aagaacgggc tgcctttcta tgagatg
//
LOCUS       DAOQUR010000058        13588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5116_length_13588_cov_9.955590, whole genome shotgun sequence.
ACCESSION   DAOQUR010000058 DAOQUR010000000
VERSION     DAOQUR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13588
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5116_length_13588_cov_9.955590"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1335)
                     /locus_tag="PKI50_10150"
     CDS             complement(271..1335)
                     /locus_tag="PKI50_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT04101.1"
                     /translation="MSLAVDIKKKLKGFSLDVSFRTEGEYLGILGASGSGKSLTLKCI
                     AGIETPDEGCIVLNGKVLYDSEKKINLKPQERNIGYLFQNYALFPHMTVEENIGMGLR
                     LPEKEKKQKVNEMIVSFHLQGLEKKYPNKISGGQQQRVALARCIVYKPDILMLDEPFS
                     ALDSHLREQLQDGVLELLKLYEGEVLMVTHSRGEVYSFCSRLVVLDNGRSILHGDTKD
                     IFRQPKLYGAARLTGCKNISKCEVLSPKNVYAADWDVILETEQKVPQQVKYVGIRAHD
                     LQITDAGQAKEKNVMECRINRIVEDVFEYNVFFENRKSNSENDNGEILYKADKQEWDN
                     IRDKDNLYLRFPESAILLLE"
     gene            complement(1416..2087)
                     /gene="modB"
                     /locus_tag="PKI50_10155"
     CDS             complement(1416..2087)
                     /gene="modB"
                     /locus_tag="PKI50_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNT04102.1"
                     /translation="MQMSPIIISLKTASASIALTFLSGLYVARWIVRMKSDKAKMILD
                     GILTLPLVLPPTVAGFLLLIVFGVNRPVGKFLLQFLGTKIVFSWSATVIAAAVISFPL
                     MYRSARGAFEQVDQELIMAARTLGMSERRIFWEIIMPLALPGVAGGGILAFARGLGEF
                     GATAMIAGNIENKTRTLPLAIYSEVAAGNMQAASYYVIIVMLISFVVVVLTNYFSIRE
                     RKYFK"
     gene            complement(2099..2875)
                     /gene="modA"
                     /locus_tag="PKI50_10160"
     CDS             complement(2099..2875)
                     /gene="modA"
                     /locus_tag="PKI50_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT04103.1"
                     /translation="MKRTVAIALFALCIVLASTGCSNNAAAKESTITVAAAASLKNCI
                     DGEVIPMFKEKNPGINVLATYDSSGKLQTQIEEGASIDVFMSAAMKQMDALNEKGLIE
                     ENSIVQLLENKIVLIVPENSTKGITAFEDILKADKIAVGDPASVPAGQYAKEALTNLN
                     IWEEISKKASLGTNVTEVLNWVAEGSADAGIVYSTDAASNKKVKVAAEAPEGTVSKVI
                     YPAGIVKASKEMDASKKFVEFLKTDEAKKVFESYGFTAIK"
     gene            3043..3564
                     /locus_tag="PKI50_10165"
     CDS             3043..3564
                     /locus_tag="PKI50_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HNT04104.1"
                     /translation="MNEDVLYTPEEIALKLRITKGTVYEMIKRGELEAHRIGRYIRIS
                     QTQFELYLLKAKGYENIYEAVISRESNGTFANIGSVKIYVDTALEGQVKVSVRPENII
                     LSMGTFISSARNVHKGTVTDIIASGSKVFVTVDIGISIKALITEKSLREMRIEKGTEL
                     YVVFKTMSVTVYK"
     gene            complement(3610..4275)
                     /locus_tag="PKI50_10170"
     CDS             complement(3610..4275)
                     /locus_tag="PKI50_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNT04105.1"
                     /translation="MHNCLENKDYNLICVIVNFGLGSDVLKLSKQHGVTGGTIFLGKG
                     TVKSAILELLELSESRKEIVLMAAEAAIEDTVLEELNRKFKFSKPNHGIAFTTSIKSI
                     LGIARCGCKIDNVSGGAENPMYNLIMAIVERGKAEAAVEAARDAGARGGTIIHARGSG
                     IHETSKIFSIEIEPEKEIALIISESTLSDKIASSISDRLNINEPGNGVIFIQSINKVY
                     GLY"
     gene            complement(4268..5755)
                     /locus_tag="PKI50_10175"
     CDS             complement(4268..5755)
                     /locus_tag="PKI50_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HNT04106.1"
                     /translation="MNIFSQKLREVLASVLPIVFIVLILNYTVLPIASNLIIRFLIGS
                     VLIIFGLTVFLVGVDIGVTPFGSLIGTSLTKNNKLWIIAVSGLLLGFFVSIAEPGLLV
                     LANQVALVTLGGISSSSIVIVVSVGLAVLVSFGFIRVVFNIPLYKVLTVSYLLIFILA
                     LFTSPEFLAIAFDASGATTGVLAVPFILALSLGIAVLNKDSKSAEKDSFGLVSIASIG
                     AIISVMILDILSKKNEYAVSLEFNILHSDSIFEPFFKLMPDILKESFIAILPLLVIFL
                     VFQRISFKLERRAYKRILIGFGYTFLGLAIFLIGVNAGFMELGSVIGYNLASFDNKWY
                     VVIIGFVIGLVTILAEPAVHVLTYQIEEVTAGYVKRSAVLVPLSLGVGIAIALSVIRI
                     LIPEIQLWHYLLPGYIISIVMTYFAPKLFVGIAFDAGGVATGPMTATFILAYIQGVAD
                     AIEWASVMRDGFGMISMVALSPIITLQILGLLFKMKSKKEVDKNA"
     gene            complement(5794..6183)
                     /locus_tag="PKI50_10180"
     CDS             complement(5794..6183)
                     /locus_tag="PKI50_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04107.1"
                     /translation="MKYIYNRKLFISVMFILLVICNVILPCSDFPILINFDKIPSIQF
                     HDETPSLKLDEENFNGVSIMLPRAQYKLKYNANSNSSIFEIIETSKFVLALQFALQLL
                     VFDRRKQIIALIISHFEGGKYKSSLSL"
     gene            complement(6388..7725)
                     /locus_tag="PKI50_10185"
     CDS             complement(6388..7725)
                     /locus_tag="PKI50_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04108.1"
                     /translation="MNIQIENAPYGERTWAGIYPLNSNRYDTYWGNLVSLSNSNSGVI
                     NSTAPVNPGLYCVRVFSQGSNIIYLSTSPSFEAAEGATAPQDNQSPVIGDLGKTDNIN
                     QVDYNVTTGHFEGQLSPLTLQAQAGNRHIKLNWTRVTDSRGVLGYNIYRSTSPGGQTE
                     QPLNASLINGTLYTDISVNVDTTYYYTVRPVYGDSSIGVRSNEVSAAPKKWAATMDTS
                     VGIAPGTTTGTTPGTTTGTTTGTTTGTGTSSQQQGDGQWNGVWKIDSGMMMLTQTGNS
                     VTGTYGEWEDNLAINGTVSGNRLTGTMDTGFGPGRFEFTIAQDGSISGWSRPDGYEAW
                     EESFEWVGVKLSDSFGNTGNAWGGVWYTDFGPMVLAQNGSGITGVYRPYCDVPVFQIE
                     GTASGNAFTGRVREGEFTGEFRFNLESGGRSFSGQFRYIGEDEWGDWNGIKKR"
     gene            complement(8008..8166)
                     /locus_tag="PKI50_10190"
     CDS             complement(8008..8166)
                     /locus_tag="PKI50_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04109.1"
                     /translation="MYKKIFSWILVLALFLQLLTPIGVQAENREYFIINKMEAAPGEP
                     YHPGRADR"
     assembly_gap    8533..8632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8633..9115
                     /locus_tag="PKI50_10195"
     CDS             <8633..9115
                     /locus_tag="PKI50_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04110.1"
                     /translation="QHWWNYYMSFFNVIAFMLISGKWFSKMYEERRGLTRAVTYFFIA
                     FVCIHFPSPILLLAGKQHYKLSFVRKIFDDYYLSSIIVSFTYHLVLCIVFVYFVCILK
                     KWYWKIVPFLTCITVLTIFEKTDILIIHNGWKFIYTILLQQISIAIFILIEKFTLKPD
                     "
     gene            complement(9124..10731)
                     /locus_tag="PKI50_10200"
     CDS             complement(9124..10731)
                     /locus_tag="PKI50_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04111.1"
                     /translation="MRISDFSGTGTLIRLFLRRDRYLLPFWILLPVILGLIVAVTFTA
                     MADKGMQSVLAEFDKDPLVSAVLGPVMSFDLSGAIVWLGTSQLALVLGIGSLLTVIRH
                     TRRDEETGRSELVRAYAVGPYANLTAALLLAAILNISSGVLIALSNIALGGAASGSFL
                     LGWTMSVVGCFFAGIGALGVQLRENSGTARGIGMAVLGLGIVMMVLNNLGGGYTLLRW
                     VTPMAWQRVTKPFAGNHGWSLIYFAAFAAIPVVIAYVLSARRDLGAGVLLARPGLSEA
                     APGLSSPLALAWRLHKRSFMGWLAGAVLYIVVFSAFSPGLSKSGGISGWLFDLGGTDW
                     TDEVGLGYVFISIGIYLVSLFVAAYAITAVLRLKKEENEGRAEMLVDKQVSRIRWMSS
                     HLIIAALCSAALLLATGIAGGLAYGLAADDLSNGFWRIFCMNVSKIPPVWILLGITAL
                     LYGLWPQITALSWVVWVSFSMLELVWEAQIIDWSLMWISPFSYAHYTIDIANLPLLPL
                     LGLLCFSAILTGIGLIGFRNRDVSTKA"
     gene            complement(10728..11636)
                     /locus_tag="PKI50_10205"
     CDS             complement(10728..11636)
                     /locus_tag="PKI50_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04112.1"
                     /translation="MNVINIQKLTKSFGSTQALKGINLTVRQGEVHGFIGPNGAGKST
                     TIRILLGILRKTSGEVSMLDGDPWKDAVRLHRRLAYVPGDVTLWPDLSGGEIIDLLGR
                     LHGGLDVSRKKQLLERFQLDPTKKSGTYSKGNRQKVALVAAFSCEAELYLLDEPTSGL
                     DPLMEAVFQECVAEIKKAGKTVLLSSHILSEVEALCDRVSIIRQGQIVESGTISQLRH
                     LTRTTVNVETGTAAAGLGKLYGVYDMSRKGLKYSFSVDSDAMTKVMEMLLPLGIKSLT
                     VEPPSLEELFMRHYGDDIREKGGVAR"
     gene            11785..12387
                     /locus_tag="PKI50_10210"
     CDS             11785..12387
                     /locus_tag="PKI50_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT04113.1"
                     /translation="MNGYERRTELKKDKIRAAALELFCMYGTDKTSINEIAQKAGVAP
                     ASIYNYFGSKEGLMKDTTINLLENGWRARKELWETDLPFPELVKRAISMNYDFIDHIN
                     LDTLSALFNTDPEIKKLADDYYKHKYPYIVSQFIEKGRREGYIRKDISVEAATIYLKM
                     YQDLFQQPEILRNRNKDLFKELCDLMLYGLAGQPITSKTE"
     gene            12554..13558
                     /locus_tag="PKI50_10215"
     CDS             12554..13558
                     /locus_tag="PKI50_10215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNT04114.1"
                     /translation="MKKYLMLGLLSLLIMVSDQPKQSEESIKIVLGGDVLLDGSVGWL
                     IENQGCSSIWGDMISILTSADFSMVNLENPLSSRGSMEKDKQFTFRGKPAYVKALESA
                     GIDMVSLANNHVLDYGKTALLDTMKHLDDAGILYAGAGQNEDAASMPVFIDKGAVSMA
                     VLCSSHVIPFVHWHAGKNKPGVASAYDPARLISEVASASEKADIVLVYLHWGKEMKSQ
                     PVQYQKNIARMLIDAGADLVIGSHPHIIQGLEFYKGKLIAYSLGNLVFTNSFKETMLL
                     SIRLSKQEMLEAHVIPCMIKSSKTMLVKDEQKKQDFIDKLNSLSPGVQVHKDGRITPD
                     "
BASE COUNT         3926 a         3167 c         2512 g         3883 t
ORIGIN      
        1 aaggaagtac ggctccgtac ccccttgtac cgatctgtca tcagctgttt caatcaaccc
       61 tgtatattct tttcatatct gcaactgaaa ccgagtgaag acatttgcag gaaaagcgaa
      121 atcagtacca acataattaa aatcctattg cgaatccttg atatattatt tataccttct
      181 ttcactaaaa atttcccgta tatcaaataa ttaactgaat tatagctccg atttccgcga
      241 aatgtcaata aagaggggca catgcccctc ttattccaat aacaaaatag cactttccgg
      301 aaatctcaga tacaaattgt ctttgtctct tatattgtcc cattcctgtt tatcagcttt
      361 atacagtatc tccccattat cattttcact attgctcttt ctgttttcaa aaaagacatt
      421 gtattcgaac acatcttcga ctatcctgtt aatcctgcac tccattacat ttttttcttt
      481 tgcctggcct gcatcagtga tttgaaggtc atgtgccctg ataccgacat atttaacctg
      541 ttgaggcact ttttgctccg tttccaatat tacatcccaa tcggcagcat aaacattttt
      601 gggggacaaa acctcgcatt ttgaaatatt tttgcacccg gtgagcctgg ctgcaccata
      661 caatttcggt tgtctgaata tatcctttgt gtccccgtgt aaaatggacc tcccgttatc
      721 aagcaccaca agcctgctgc aaaaactata tacttctccc ctgctgtgcg taaccatcaa
      781 aacttctccc tcatacaatt ttaacaattc caaaaccccg tcctgaagct gttctctcag
      841 gtgcgaatcc aatgcagaaa acggctcatc cagcatcaat atatccggtt tgtatacaat
      901 gcatcttgcc agtgcaaccc tttgctgctg tccccccgaa atcttgtttg gatacttctt
      961 ttccagtccc tgcaaatgaa aggacactat catttcatta accttttgtt tcttttcttt
     1021 ctccggaagg cgcaatccca tgccgatgtt ctcttctacc gtcatatgag gaaacagggc
     1081 ataattttga aataaatagc cgatattcct ttcctgcggc ttaagattga tcttcttttc
     1141 ggaatcatac aggactttac cgttaagaac aatacagcct tcatcagggg tttcaattcc
     1201 ggcaatacat ttcagcgtca agcttttccc gctgccggaa gcccccagga ttccaagata
     1261 ctccccctct gttctaaaag aaacgtcaag agaaaaacct ttcagttttt tctttatatc
     1321 aaccgccaag ctcatatgct tctcccctgt ttgattattt ccattacttc tacttgtttt
     1381 tcactatacc acttactttg ctttccccta taccgctact taaagtattt tctttccctt
     1441 atagaaaaat aatttgtcaa aacaacaaca acaaaggata ttaacataac aattattaca
     1501 taataagagg cagcctgcat attcccggca gcaacctccg aataaattgc caatggaagc
     1561 gtcctcgtct tattttctat gttacctgcg atcatagccg tagcgccaaa ctcgcccaag
     1621 cccctggcaa atgccaatat cccgcctccg gcaacacccg gtaaagcaag gggcattatt
     1681 atttcccaga aaatccttct ctcggacatt cccaatgtcc ttgctgccat aattaattct
     1741 tggtctacct gttcaaaggc acctctggca gaacggtaca taaggggaaa tgaaattacc
     1801 gcagctgcta ttacggtggc actccaggag aaaactattt ttgtacccaa aaattgcaga
     1861 aggaatttgc cgacaggcct gtttacacca aaaacaatca gaaggagaaa tcctgctact
     1921 gtcggcggca aaaccagagg taatgttaat atgccatcca atatcatctt tgctttgtcg
     1981 gatttcatcc ttacaatcca tcttgcaaca tatagccctg acaaaaatgt taaggctata
     2041 gatgcggaag cggttttcaa tgagattatt atcggtgaca tttgcatatc atacactcct
     2101 acttgatcgc tgtgaaacca tatgattcaa atactttctt tgcttcatcg gttttcaaga
     2161 actcaacaaa tttttttgat gcatccattt ctttagaagc tttaactatg cctgccggat
     2221 aaatcacttt agaaaccgtt ccttccggcg cttccgcagc aacttttact tttttgtttg
     2281 aagctgcatc ggttgaataa acgataccgg catcggcgct gccttcagct acccagttca
     2341 gtacttccgt aacatttgtt ccaaggcttg cctttttaga tatctcttcc caaatattaa
     2401 ggtttgttaa tgcttctttt gcatactggc ccgccggtac gcttgccggg tcgccgacgg
     2461 ctattttatc agcctttaaa atatcttcga aagcggttat tccctttgtg ctgttttccg
     2521 gaactattag cacaatcttg ttttccaaaa gctgaacaat agagttttcc tcaattaagc
     2581 ctttttcatt caatgcatcc atttgcttca tagctgctga cataaacaca tctatggacg
     2641 caccttcttc aatttgtgtc tgtaattttc ccgagctgtc ataggttgcc aatacattga
     2701 taccgggatt tttttcttta aacatcggta tcacttcacc gtctatgcag tttttcaagc
     2761 ttgccgcagc agcaacggta atagttgatt cttttgcggc agcattgttg ctgcagcctg
     2821 tcgaagccaa tactatgcac aaggcaaaca acgcaatagc aactgttctt ttcatttttg
     2881 accctccttt tttgaaacca attaaaccta actaaaacta actcaaccta actaaaaata
     2941 gtttaccaga attaatccga tatgtaaaca ttctatgtaa tcatattgct ttatggtaaa
     3001 atggtattat gtaattatag aactgcaaag aaggtgagtt aattgaacga ggatgtctta
     3061 tatactccgg aggagattgc tctgaagctt aggataacaa aaggaacagt atatgaaatg
     3121 attaaaagag gtgagctgga ggctcatcga atagggaggt atattcgcat atctcaaact
     3181 caatttgaat tatacctgtt aaaggcaaaa ggctatgaaa atatatatga ggcagtcata
     3241 agccgggaga gcaacggaac ctttgcaaat atcggatcgg ttaaaattta tgtcgatact
     3301 gctttggaag ggcaggttaa agtatctgtc cgcccggaaa atattatatt aagtatgggt
     3361 acatttatta gcagcgcgcg aaatgttcat aaaggaacag taactgatat tattgccagc
     3421 ggctccaaag tatttgtaac cgttgacata ggtatttcga ttaaagcact cataaccgaa
     3481 aaatcattaa gagaaatgcg tattgaaaaa ggaacagaac tctatgtcgt tttcaagact
     3541 atgtccgtga cagtctacaa atgaagacag gggacaagtg cacttcagta cctgtcccct
     3601 gtctataatt taatataaac catatacttt atttatactc tgaatgaaaa tgacaccatt
     3661 gccgggctca ttaatattaa gccggtcgct gattgaagaa gcgattttgt cactcaacgt
     3721 gctctcggat attatcagag caatttcttt ttccggttct atttctattg agaatatctt
     3781 actggtttca tggatgccgg agcctcttgc gtggataatt gtccctcctc ttgcccctgc
     3841 gtcccgtgct gcttcaactg cagcttccgc tttgcctctt tcaactattg ccataataag
     3901 attatacatg ggattctccg cacctccgct tacattgtca atcttacagc cgcatctggc
     3961 aatgcccaat atacttttta ttgaagtagt aaatgcaatg ccgtgattcg gctttgaaaa
     4021 tttaaacttc ctattaagct cttcaagtac agtatcttct atagctgctt ctgctgccat
     4081 cagtacaatt tccttcctgc tctcggacag ttccaaaagc tccagaatag cacttttcac
     4141 cgttcctttc ccaaggaaaa tggttccccc ggttaccccg tgctgcttgg atagtttcag
     4201 aacatcactg cccaatccga aatttacgat tacacagatt agattatagt ctttattctc
     4261 aaggcaatta tgcattttta tctacctcct ttttggactt catcttaaat agcagcccca
     4321 atatttgaag agtgataatg ggggacaaag ccaccatgga aatcattccg aagccatccc
     4381 tcataacact tgcccattca atggcatccg caacgccttg aatataagcc agtataaaag
     4441 ttgcagtcat agggccggtt gcaacccctc ctgcatcaaa ggctatgcct acaaagagct
     4501 tcggtgcaaa atatgtcatg actatcgaaa ttatatatcc gggtaaaaga taatgccata
     4561 actgaatctc aggaatcaat atccttatta ctgataaagc aatggcaatg cctactccaa
     4621 gagaaagcgg cactaatacg gctgatcttt taacataacc ggcagttacc tcttctatct
     4681 gatacgtcag aacatgaact gccggctcgg ccaatatcgt caccagaccg atgacgaaac
     4741 cgataattac aacataccac ttattgtcaa aggaagcaag attatatcct atcacacttc
     4801 ccagctccat aaagcccgca ttgacaccga tcagaaatat tgcaagcccc aggaatgtat
     4861 aaccgaaccc tattagtatc cttttatatg ctctccgctc cagcttgaag gatattcttt
     4921 gaaaaaccag gaatataaca agcaagggta aaattgcgat aaaactctct ttcagtatat
     4981 ccggcatcaa cttgaaaaag ggctcaaaaa ttgaatccga atgtaatata ttaaattcaa
     5041 ggcttacagc atactcattc ttttttgaca gtatatccaa aatcattacc gaaattatag
     5101 caccaataga tgctatggaa accaatccaa aactatcttt ttccgcagat ttgctatcct
     5161 tgttcagaac agctatcccc agggaaagcg caaggataaa gggtacggca agtactccgg
     5221 tggtagcgcc tgatgcatca aaggctattg ctaaaaactc aggtgaagta aataatgcca
     5281 gtataaaaat taataaatat gatacagtca ataccttata cagaggtata ttaaaaacaa
     5341 ctctgataaa accaaatgaa accaataccg ccagcccgac agacaccact ataactatac
     5401 tgctgcttga tatcccgcct aatgtgacta aagcgacctg gttagccagg acaagcaatc
     5461 ccggctccgc aatcgacaca aaaaaaccga gaagcaaacc ggaaacggca ataatccata
     5521 gcttattatt tttagtaagg cttgtgccta taaggctgcc aaaaggagta actccaatat
     5581 ccactccaac cagaaaaaca gttaatccaa aaattatcag tactgaaccg attaaaaatc
     5641 taatgattaa atttgatgca ataggcaaga cagtatagtt tagtatcaac acaataaaaa
     5701 ctattggaag cactgatgcc aatacctctc taagcttttg actaaatata ttcacaggta
     5761 catcacttcc ttttcattaa cttctcataa gtgttacaag gaaagtgagc ttttgtattt
     5821 tcctccttca aagtggctta ttattaatgc tattatctgt ttccgtctgt caaataccag
     5881 caactgcaag gcaaattgca gtgctaacac aaattttgaa gtttcaatta tttcaaatat
     5941 actgctattg gaatttgcat tatatttcag cttatactgc gctcttggca gcataatcga
     6001 tacaccgtta aagttttctt catccaattt gaggctcggg gtttcatcgt ggaattgaat
     6061 gctcggtatt ttatcgaagt taattaatat gggaaaatcg ctgcaaggca gaattacatt
     6121 acaaataacc aacaatataa acattacaga tataaaaagt tttctgttat aaatatactt
     6181 cataataaaa tccctcatta tgcatatcta atgaaatttg cttaaaagtt ttaataaaca
     6241 gtggtttatt aaaaatattg tttaatttgt aacaatatct taatgaatct cacattatct
     6301 cgactttata atatcataaa acctgaccta atacaatcaa ggtcaaggta tctataaaaa
     6361 aatgcgtctc tcgacgcatt tttttcacta tctcttcttt atcccgttcc aatcccccca
     6421 ctcatcttct ccgatgtagc ggaactgccc gctgaaggac cttcctccgc tttcaaggtt
     6481 gaatcggaat tctcctgtaa attctccttc ccttactctg cctgtaaaag catttcctga
     6541 agctgtccct tctatctgaa aaacaggtac atcgcaatat ggcctatata ctcctgtgat
     6601 gccggagccg ttctgggcca acaccatagg gccgaagtcc gtgtaccata ctcctcccca
     6661 tgcattgcca gtgtttccga aggaatcgga taatttaact cctacccatt cgaaggattc
     6721 ctcccaagct tcataaccgt caggacggct ccagccgctt atgcttccgt cctgagcaat
     6781 tgtaaattca aacctgccgg gaccaaagcc ggtgtccatg gtacccgtta gcctgtttcc
     6841 gcttacagta ccgtttatcg ccaagttgtc ttcccattca ccgtaggtgc cggttacact
     6901 gtttcccgtc tgtgtgagca tcatcatgcc actgtctatt ttccatacac cgttccactg
     6961 gccatccccc tgctgctggc tcgaagtgcc tgtacctgtt gttgtacctg ttgttgttcc
     7021 agttgttgta ccgggtgttg tgcctgttgt tgttccaggc gctattccaa cggacgtatc
     7081 catagtagcc gcccattttt taggtgcggc agacacctca ttggatctta cccctatgct
     7141 gctgtcgccg taaacgggcc tgacggtata gtaataagtc gtatcgacgt ttacgctaat
     7201 gtcggtatat aaggtaccat tgattaatga agcgttaaga ggctgctctg tctgccctcc
     7261 cgggcttgtc gatctgtaga tattgtatcc caatactccc cgtgaatcgg taactctggt
     7321 ccaattcagc ttaatatgcc tgtttccggc ctgtgcctgc agtgttagcg gagataactg
     7381 gccctcaaaa tgccctgttg tgacattata atcaacttga tttatattgt ctgttttacc
     7441 caaatcccct attacagggc tctgattgtc ttgcggtgcg gtcgctccct cagccgcttc
     7501 aaaggaaggg ctggttgaaa ggtatataat attgctgccc tgactgaata cccttacgca
     7561 gtacaaaccc ggattaaccg gcgcagtgct gttgataacc ccgctgttgc tgttgctcag
     7621 cgaaaccaga tttccccaat aggtatcata tctgttgctg ttcagagggt atatccctgc
     7681 ccaggtacgc tcaccgtatg gtgcgttttc aatttgtatg ttcaattgcc ctgttgggtt
     7741 tattttgtca ggtatgttcc taaatacggt attatggggc attacataaa acggagaact
     7801 tacgcctgtt cttacatata ccccgtttgt aaagtaaacg gatctgaatt ggtatgcccc
     7861 aggtatcata ggcatgtcaa aaaatacatt gccggttgta ttatttccag tactttgcag
     7921 gtccgaccaa tattcatcag tctcaccaat actgtaaagc ccaatataag agttgctctt
     7981 ttcaggtata ttttcataac tgacaattca tctgtccgcc cggccagggt gatatggctc
     8041 cccaggcgct gcctccattt tattgattat gaagtattcc ctattctcag cctgaactcc
     8101 tattggtgtt aaaagctgaa gaaataacgc caataccaga atccacgaaa atattttttt
     8161 atacaaattt acctccttca aatttctcgt ttctgcctgc cccctttatc cctggggtat
     8221 gtggctgttt ttcctgctgc tgttatttca taaccattta tttcttgatc agacaatatt
     8281 attatttatt ttattagacg ttaaatcgta attataggta aaatttgatc aaaatcagta
     8341 ccaaaaatgt tattttgata aatactccca acctgcagca gattgaaggg ccgggaattc
     8401 agcccctaat ctgaataatt gatatccgca aatcaaataa tattgcttaa caaggcacat
     8461 ttctgcagaa tttccttttt taataatgtg ctgtatttac aagtaaagca ggtataaaat
     8521 gtcaaacgta atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagcattg
     8641 gtggaactat tacatgtcct tcttcaatgt tattgctttt atgctgattt ccgggaagtg
     8701 gttttccaaa atgtatgaag aacgccgcgg gctaaccagg gctgtaacat atttttttat
     8761 tgcatttgta tgcatacact ttccgtctcc aatattgctg ctggcaggta agcagcatta
     8821 caaactaagc tttgtcagga aaatatttga tgattattat ctgtcaagca ttattgtttc
     8881 atttacatac catcttgtct tatgcatagt atttgtatac tttgtatgca tacttaaaaa
     8941 atggtattgg aaaatcgtac cttttctcac ttgcatcaca gttctgacaa tatttgaaaa
     9001 aacagacatc ttgattattc ataacggttg gaaattcatt tataccatac tattgcagca
     9061 aattagcata gcaatattta ttctgattga aaaattcacc ttaaaaccgg attaaagctg
     9121 aatttatgct tttgttgaaa catctctgtt cctgaatcca atcaatccaa tccccgttag
     9181 tatggcagaa aagcaaagca gcccaagtag cggaagcagc ggtaaatttg ctatatcaat
     9241 ggtatagtgg gcatatgaga agggagaaat ccacatcagt gaccaatcaa ttatctgagc
     9301 ttcccataca agctccaaca tactaaacga tacccaaacc acccagctca gagctgttat
     9361 ttgaggccat aaaccgtaca ataaagctgt tatgcctaac agtatccata caggcgggat
     9421 ttttgaaacg ttcatacaaa aaatacgcca gaatccattg cttaaatcgt ctgctgcgag
     9481 tccataagcc agtcccccgg caatacccgt tgccagcagc aatgcagcag agcataaagc
     9541 tgcaataatt aaatggctgc tcatccatcg aatccggctc acctgcttat ccaccagcat
     9601 ctccgctctg ccctcatttt cctccttttt caggcgcaat acagctgtta tggcataggc
     9661 agccacaaaa agcgacacca gatagatgcc gatactaata aagacatatc ccaagcccac
     9721 ttcatcggtc caatccgtac ctcccaaatc gaacagccag ccgcttatcc cgccggattt
     9781 ggataagccg ggagagaagg ctgaaaacac aacaatatac aatactgctc ccgccagcca
     9841 gcccataaag cttctcttat gcagcctcca ggctaatgcc aaagggctgg aaagaccggg
     9901 agccgcttcc gaaagccccg ggcgggccaa aaggacacct gcccccagat ccctgcgggc
     9961 agaaagcaca tatgcaataa cgacaggtat ggccgcaaaa gcagcaaaat atatcagact
    10021 ccagccatga ttgccggcaa agggtttggt cacccgctgc caggccatgg gagtaaccca
    10081 ccttaataag gtatagcccc ctcccaagtt attcaaaacc atcatcacta ttcccagccc
    10141 taagactgcc atgccaattc cccgtgcagt tccactgttt tcccgcagct gtacacctaa
    10201 agcgcctatc ccggcaaaaa agcacccaac aaccgacatg gtccatccta acagaaagga
    10261 acctgatgca gcacccccaa gggcaatgtt actcaaggcg atcagcactc ctgacgaaat
    10321 atttaaaata gcggcaagaa gcaatgcagc agtcaaattg gcataagggc ctactgcata
    10381 tgcacgaacc agctcgctgc ggcctgtttc ttcatctctg cgggtgtgcc ggattacggt
    10441 aagcaggctg cctattccca aaacaagcgc cagttgtgaa gtgcctagcc atacaatagc
    10501 tcctgaaagg tcaaaggaca tgaccgggcc aagaaccgca gaaactaacg ggtctttatc
    10561 aaattcagcc aatacgctct gcattccctt atcggccata gcggtgaaag taactgccac
    10621 aatcaaaccc agaatcacag ggagtaatat ccagaagggc agcaagtacc tgtcccggcg
    10681 cagaaacaac ctgataagcg tcccggttcc cgaaaaatca ctgattctca tctcgcaact
    10741 cctccttttt cacgaatatc gtcaccgtaa tggcgcatga aaagctcctc caaacttggc
    10801 ggctcaaccg tcaaggactt gattcccaat ggcagcagca tctccattac cttcgtcatg
    10861 gcatcggaat ctactgaaaa actatattta aggcccttcc gggacatatc ataaactcca
    10921 tataattttc ccagcccagc agcagcggtg ccggtctcca cattaacggt ggtacgggta
    10981 agatgccgaa gctgtgaaat ggttcccgat tccacaatct gcccttgccg gataatgctt
    11041 accctgtcgc atagagcctc cacctcagac agaatatggc ttgacagcag cacggtctta
    11101 ccggcttttt ttatttccgc tacgcattcc tgaaagactg cctccattaa aggatcaagc
    11161 cctgaggtgg gctcatccaa aagatacaat tccgcttcac aggaaaaggc tgccaccaaa
    11221 gctaccttct gccggttccc tttggaatag gttccgctct ttttggtcgg atcaagctga
    11281 aagcgttcca gcaattgctt ttttcttgaa acatcaagcc ctccgtgcag ccgtcctaga
    11341 aggtcaatga tttcacctcc ggacagatcg ggccacaggg taacatcacc cgggacataa
    11401 gcaagccggc gatgcagcct gacagcatcc ttccatggat caccgtcaag cattgagact
    11461 tctcccgatg tttttctcaa aatccccagc aggatgcgaa tagtcgtgga cttcccagct
    11521 ccgttggggc cgataaatcc gtgtacctcc ccttgccgca ctgtgagatt gattcccttt
    11581 aacgcctgag tcgagccaaa agacttggtc agcttttgaa tatttatcac attcatgtac
    11641 tgccctcccg atttatatat tttataatat cttgttatta tatctctata ttatctaatt
    11701 ttaatgcttc tgtcaataag ttttatatat tttatattga ctcattacaa aacttctagt
    11761 ataattgaga cggggtgaaa aaccatgaac ggatatgagc gcagaacaga attgaaaaaa
    11821 gacaaaatac gtgctgccgc attagagctc ttttgcatgt acggtacgga taagaccagt
    11881 ataaatgaaa tcgcacagaa ggcgggggtt gcaccggcca gtatttataa ttattttggg
    11941 agcaaggaag gccttatgaa ggatacaacc ataaacctcc ttgaaaacgg ctggagagca
    12001 agaaaagagc tgtgggaaac cgacctgcct tttcctgagc tggtgaagcg tgccatatca
    12061 atgaattatg attttattga ccatatcaat ctggatactc taagtgcatt gtttaatacc
    12121 gatcctgaga taaagaagct ggcagatgat tattataagc acaagtatcc ttatatcgta
    12181 agccaattca ttgaaaaagg ccgaagggaa ggctatatac gcaaagatat ttcagtagaa
    12241 gcagctacaa tttatcttaa aatgtatcag gacctgttcc agcagcctga aatactgcgg
    12301 aacagaaaca aagatttgtt taaggaatta tgtgatttaa tgctgtacgg tttggcgggg
    12361 cagcctatta cctccaaaac ggagtaagga aaaaaacata aaagcctctc aattattaat
    12421 caggaaatac agcttcagga caaggtatct tcaccttgtc ctattatttt ccagttttca
    12481 gacaaggcat atgaggcaag atattaacat acaatgttag tgacattcct gggtatggga
    12541 gatgattgaa gctgtgaaaa aatacttgat gctggggctt ctttcgctgc tgattatggt
    12601 ttctgatcag ccgaagcaat cggaagaaag cataaaaatt gtacttggcg gagatgtact
    12661 tctcgacggg tctgtgggct ggcttattga aaatcaggga tgcagcagca tatggggaga
    12721 tatgattagt atcctcactt ccgctgattt ttcaatggtg aatcttgaaa atcccttaag
    12781 cagcaggggc agcatggaaa aagacaagca gttcacattc aggggaaagc ccgcatatgt
    12841 aaaagctttg gaatctgcgg gaattgatat ggtttcactg gctaacaacc atgtcctgga
    12901 ttacggaaaa actgctctcc tggatacaat gaagcatctg gatgatgccg gaattctcta
    12961 tgccggtgcg ggacaaaatg aggatgccgc aagcatgccg gtatttattg ataagggagc
    13021 agtaagtatg gctgttctgt gctccagcca tgttattccc tttgtgcact ggcatgcagg
    13081 caaaaacaag cccggggttg caagcgcata tgatcctgca aggcttatat cagaggttgc
    13141 atcggcttct gaaaaggcag atatagtcct ggtatacctt cactggggaa aagaaatgaa
    13201 atcccagcct gtccaatacc agaaaaacat tgcccggatg ctgatagatg ccggcgcgga
    13261 tctggtaatt ggttcacacc cccatattat tcaaggcctt gaattttata agggaaaact
    13321 aatcgcctac agcctgggta atcttgtctt caccaacagc ttcaaagaaa caatgcttct
    13381 cagtatcagg ctttcaaagc aggaaatgtt ggaagctcat gtgattccat gcatgattaa
    13441 atcctcgaaa acaatgcttg ttaaagatga acagaaaaag caggatttta ttgataagct
    13501 gaattcactg tccccgggtg tacaggtgca taaggacgga agaataaccc cggactgatt
    13561 taatctcctt taattatttt ccgtttaa
//
LOCUS       DAOQUR010000059        13236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5285_length_13236_cov_10.584402, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000059 DAOQUR010000000
VERSION     DAOQUR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5285_length_13236_cov_10.584402"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKI50_10220"
     CDS             complement(<1..1076)
                     /locus_tag="PKI50_10220"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNT04115.1"
                     /translation="MSLNSLKELKIGNLTARLPIIQGGMGVGISLSGLASAVANEGGV
                     GVIAAAGIGMLDYDFSTKFIEANIARLRSEIRKAREKSKGIIGINIMVALSNYAEFVK
                     ASIEEGIDIIFSGAGLPLNLPQYLEEKYETKLVPIVSSARAAGIICKKWLDKYNYLPD
                     AIVVEGPKAGGHLGFKEDQIFDPDYSLEKILPEVVDEVKAFEDKSGKSIPVIAAGGIY
                     SGEDIYNIMKLGASGVQMATRFVTTEECDASMDFKKAYINSKKEDVSIIKSPVGMPGR
                     AIRNSFTEDISKGANKPYKCPYHCIVTCDYKNSPYCIALALTNAQKGKLENGFAFAGV
                     NAYRADKVITVKELMDTLQNEYSEA"
     gene            1344..2213
                     /locus_tag="PKI50_10225"
     CDS             1344..2213
                     /locus_tag="PKI50_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNT04116.1"
                     /translation="MVRVIVDSSCDLPDELLEEYEIKSVSQRVYINNREYLDKVTINA
                     KEVYEAMKKGIIPQTSLPRPMEIYGHFKQCCQEGNDFIYIALSSKFSGTYQLAVSLMQ
                     ELQGQYDKVRMDVIDSKSGSTAIGLIALQAAKLARAGLGFDMIVRQICELADHVEHIF
                     MVQDLSWLIRGGRISRTEGIIGSILNVKPILHVKDGAAEILEKVRGRKKALDSIVNIM
                     EERIKDFPDQTIGISYAWDQDIAYEIRDMIKQRLEVKNFVINEIGAALASHLGIGGVG
                     VFFFNREPDFYME"
     gene            2272..2796
                     /locus_tag="PKI50_10230"
     CDS             2272..2796
                     /locus_tag="PKI50_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04117.1"
                     /translation="MSLRVGFDNNLISQNSGYASGASGQAAMSPDGKKSVEQGQTIDS
                     IRNDKLMKKIGAVECQTCSNRKYVDGSDDAGVSFKTPTHVSPENSMAAVASHEQEHVS
                     REGAKARAEGREVVSQSVRVFTDVCPECGRVYASGGKTTTITKADNKNTDYFMDNMRK
                     SMGIHFGKYIDKRI"
     gene            complement(2793..3782)
                     /locus_tag="PKI50_10235"
     CDS             complement(2793..3782)
                     /locus_tag="PKI50_10235"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNT04118.1"
                     /translation="MLKIPERIHLANLPTRIEKLDKLTSVLGGPEIYIKRDDQTGTEL
                     SGNKVRKLEYSVKEALDMGCNYLITCGGVQSNHCRATAAAAAKLRMKSCLVLRGDAGS
                     EAEGNLLIDRLLGADIRFITAEEYSDQRTEIMEAIKRETDDKGFKAYIIPEGASNGIG
                     SFGYYTAMEEIIEQEKELGIHFDCIVLATGSGGTYSGLWLANKLLGHSSDVCGICVGG
                     DAGYFKAIIPEILFESMEYMDVKIPVSPEDINIIDGYVGRGYALSRPEELRFIHGFAE
                     LEGIILDPVYTGKAMYGLTEEIKKGKFKNYKNILFIHTGGLFGLFPQGDLFKF"
     gene            3964..4833
                     /locus_tag="PKI50_10240"
     CDS             3964..4833
                     /locus_tag="PKI50_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT04119.1"
                     /translation="MSDIAGKGCEILVPFSERKPLPEIERSLIKKYRKHIWTRFMKAV
                     KEFELVKEGDRIAVGVSGGKDSMLMAKLMQELQRHGDASFELEFIAMDPGYHENIREL
                     LIENCNYLEIPIHIFESGIFKIVDKIAKDYPCYMCAKMRRGALYGKAMELGCNKLALG
                     HHFNDVIETTMLNILYTGSFKTMLPKLKSTNFKGLELIRPLYYVDELYIERFTQSSGI
                     WPLNCACMVAAGKIANKRCEIKALIKELKEKIENADTNILRATQNVNLEAILGWHKGG
                     QQYSYLDFYEEQE"
     gene            4915..5373
                     /locus_tag="PKI50_10245"
     CDS             4915..5373
                     /locus_tag="PKI50_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HNT04120.1"
                     /translation="MAIANIKGGPLAERLKGTVLFTNVPGGVWVCVHVSGLSDYKAAK
                     DGGQPVGPHGFHIHENGSCEVGDPEEPFKAAGGHWNPANQPHGNHAGDFPVLFSNKGM
                     ARMCFFTDKFKTRDIIGKAVIIHESPDDYRSQPAGNAGKRLGCGVIREGR"
     gene            5377..6000
                     /locus_tag="PKI50_10250"
     CDS             5377..6000
                     /locus_tag="PKI50_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNT04121.1"
                     /translation="MSFVQNPVRISTRRITAQDNMVTIDYPVVKGLESPAAQHRINMT
                     IAAAVNMLMREQGFYEKQKTEMLGWYEIKTNERGILSLTIGNYAFTYPSAHGLTIVKG
                     LTFDVNSGYSIELKDLFKPEADYDKVLSGIVEQQITDRNIDLLDEFKGIRPYQDFYIA
                     DKSLVLFFQLYEITPYYMGLQYFPISVYQIQDIIAEEGALGIMAAGD"
     gene            6147..7289
                     /locus_tag="PKI50_10255"
     CDS             6147..7289
                     /locus_tag="PKI50_10255"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNT04122.1"
                     /translation="MIKRIVAAPDSFKGSLTAKEAAEAMEAGIKRYDNSIEVVKVPMA
                     DGGEGTVQSMVDATGGHMISVTVKGPMLSEVEAFYGILGDGSTAVIEMAAASGLPLVK
                     PEERNPLIATTYGTGELIRHALDMGCRRIIIGIGGSATNDGGAGAMQALGVRLLDDFG
                     NDIGFGGGSLGSLYRIDINNLDPRIKDTVITVACDVDNPLCGHKGASQVFGPQKGADD
                     AMIEELDRNLAHYADLVESTLGVKIKDYPGAGAAGGLGGGLLAFLSATLKKGVDIVIE
                     TVELEKKIRDADLVITGEGRMDNQTIYGKTPFGVAKAAEKYGIPVIAICGSIGKGAEV
                     LYDNGFASIFSIIDKPMCIDEAMKKGSELIENCSERVIRALSITEQ"
     gene            7313..8749
                     /gene="glgA"
                     /locus_tag="PKI50_10260"
     CDS             7313..8749
                     /gene="glgA"
                     /locus_tag="PKI50_10260"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNT04123.1"
                     /translation="MDSINVLFAASEAVPFAKTGGLADVAGTLPRELVKLGADVRLII
                     PKYKSIPRNFVEAMEYIGYVYVDLAWRHQYCAVLKLEYQGVITYFIDNEYYFNRDCLY
                     GHFDEAEQFAFFSRAVLKVLPLIDFKPDIIHCNDWQTGVVSAFLKAGFGHIGFLKDIK
                     TVYTIHNLKYQGMFPKEVLGDILGLSWDYFNPEGLELHDCVNYMKAGIAYSDIVTTVS
                     KTYAEEIKTDFFGENLNGILTKRSESIFGILNGIDYLENDPETDTRIYKSFNAEDLEG
                     KYENKKMLQESLGLPQREEVPVIGVVSRLVGQKGFDLIAHVLDEMLKMDIQLVVLGTG
                     DSNYENMFRNAAERYPGKVSANIKYDGVLAQRIYAGSDMFLMPSLYEPCGLSQIFSLR
                     YGTVPIVRETGGLKDTVMPYNEFTGEGTGFSFANYNAHEMLDTVKRAISFYKNKYIWE
                     KIVKSGMKQDFSWSKSAKEYMELYEKMV"
     gene            complement(8814..10190)
                     /locus_tag="PKI50_10265"
     CDS             complement(8814..10190)
                     /locus_tag="PKI50_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNT04124.1"
                     /translation="MSKIPYRVYLSEKEIPRHWYNLRADMKENHDPYLNPGTMKPAEI
                     NDLYPVFCEELAKQEMDSETRYVDIPDEILEFYKIYRPSPLCRAYNLEKALDTPAKIY
                     FKFEGNNTSGSHKLNSAIAQVYYAKKQGIASLTTETGAGQWGTALAEACAYFGMPLTV
                     YMVKVSAEQKPYRKSIMETFGAKVIASPSNLTEAGRSILAKNPDTGGSLGCAISEAIY
                     HAVNTKNCRYVLGSVLNQVLLHQSIIGLETKLAMEKIEEYPDIIIGCAGGGSNLGGLI
                     APFMQDKLTGKANPCFIAVEPASCPSLTRGRYAYDFCDTGKITPLAKMYTLGSGFIPS
                     SNHAGGLRYHGMSPMLSKLYHDGYINEARAEEQTRVFEAAAAFARYETILPAPESSHA
                     IRAVIDEALKCRETGEAKTILFGLTGTGYFDMQAYIQYNSGKMSDYIPTDEDLQKGFS
                     ELPNIPGL"
     misc_binding    complement(10252..10495)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10609..10857)
                     /locus_tag="PKI50_10270"
     CDS             complement(10609..10857)
                     /locus_tag="PKI50_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04125.1"
                     /translation="MANSYGSVNPAVAAAGCLQGKAVSPFADLCHNNGFLLLLLLFIL
                     LAAGPSWGIDNNFFFIIILAIIAFGGFGRTFGFNGAPV"
     gene            11060..11497
                     /locus_tag="PKI50_10275"
     CDS             11060..11497
                     /locus_tag="PKI50_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNT04126.1"
                     /translation="MGAEVECIQKMLEDHGIAHTRQREEILSLFIIESKHYKPDEIFK
                     LLRGKGIGIATVYRTLEMLRECGIIKEISVGKERFYELKINESKHIYVHFKCNKCGKF
                     FDYYNVEAVQEYVSAVDQVEKNMNVKVNDVNINLYGICSHCKS"
     gene            11687..11938
                     /locus_tag="PKI50_10280"
     CDS             11687..11938
                     /locus_tag="PKI50_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04127.1"
                     /translation="MEINDMDMHQLEDVLYETSKFNDNDFSTFEEALLDMYGGINTQN
                     LKRIIDFAEHELGIRSDSDLTHLDRNQKKKIVEFASTLE"
     gene            complement(12095..13168)
                     /locus_tag="PKI50_10285"
     CDS             complement(12095..13168)
                     /locus_tag="PKI50_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HNT04128.1"
                     /translation="MWNKRNKKILKRLKLTILIPLGMLLVYFASRNPELVERLYSNGI
                     YKPLGSILSKAFGILPFSAAEFIVILVPAVFIVYTVRALINMQLIKYLTNILVIVSIV
                     FFSFIGVWGLNYYRMPFASIAGLEVRPASVYELESLCLDLIERANALRLSVSVDSEGN
                     VAIPGSPRDILKNCHKGYESVAGKYPELSGSYSHPKPVLLSRLMNYTGLCGVYFPFTG
                     EANVNIAIPESTLPYTASHEMAHQRGFSREDEANYISYLACSAHPDVNYRYSGILLAL
                     TNSMNALHDTDQALFKKLSESYSDEVRHDLASINEFWAGYEGPVERATDRINDKYLKA
                     NNQKDGVKSYGRMVDLLIAEHRK"
BASE COUNT         3950 a         2553 c         3130 g         3603 t
ORIGIN      
        1 gcttcagaat actcattctg caatgtatcc ataagttcct tgacagttat gaccttgtct
       61 gccctgtacg catttacacc cgcaaaggca aagccattct caagcttgcc tttttgtgcg
      121 tttgtcagcg caagagcaat gcaataggga ctgttcttgt aatcgcaagt gactatgcaa
      181 tggtatggac acttgtacgg cttattggcg cctttcgaaa tatcctcggt aaaggaattt
      241 cttattgccc gtcccggcat tcccacaggg cttttaataa ttgaaacgtc ttctttcttt
      301 gaattgatgt aggctttttt gaaatccata gaggcatcac attcttctgt tgttacaaag
      361 cgagttgcca tttgcactcc tgaagctcca agcttcataa tattataaat atcttctccg
      421 gaataaattc ctcctgcggc aatcacaggt atgcttttgc ctgatttatc ctcaaaggct
      481 ttaacttcat ccactacttc aggcagtatt ttttcaaggg aataatcagg atcaaatatc
      541 tgatcctcct tgaaccccaa gtggcctccg gccttaggcc cttccactac tattgcatcg
      601 ggaagatagt tgtatttgtc cagccatttt ttgcagataa tacctgcggc tcgtgccgaa
      661 gatacaatcg gaaccagctt ggtttcatat ttctcttcca gatactgagg aaggttcaaa
      721 ggaaggccgg cgccggaaaa aatgatatct attccttctt caatggaggc ttttacaaat
      781 tcagcatagt tggacaaagc aaccattata ttaattccga tgataccttt cgatttttcg
      841 cgggcttttc tgatttcgct tctcaatctc gcaatattgg cttcaataaa ttttgttgaa
      901 aaatcatagt ccaacattcc tatacctgca gcagcgatga cacctacacc gccctcattt
      961 gccactgccg atgcaaggcc ggacagtgat attcctactc ccatgcctcc ctggataata
     1021 ggcagtcttg cagttagatt gcctattttc aattctttaa gtgaatttaa gctcataaaa
     1081 acctcccata tcaaacgaat aagtaagaga atattctgat gattattatc tcataattat
     1141 aaccttataa tgtagtatac cataactcat caaatgtgaa tatcaaagtt tttataaaat
     1201 ggggtagttc cttgtcaaat actccggata caagttccgg gtattcacca agcagcagga
     1261 caaagtgagt cacaccagat accatgtaca tatattgaat taggataagt tgacaagcat
     1321 tattctaatt ggagggttcg tagatggtaa gagttattgt agacagttcc tgtgacctgc
     1381 cggatgaact gctggaggaa tatgaaataa agtcagtatc ccaaagagtt tatattaata
     1441 acagggagta tctggacaag gtcacaataa atgcaaaaga ggtctatgag gccatgaaaa
     1501 agggtattat tccgcaaacc tccctgccga ggcctatgga gatatacggc catttcaaac
     1561 agtgctgcca ggaagggaat gacttcatat atattgctct ttcctcaaag ttctcgggga
     1621 cttatcagtt ggctgtgtct ttgatgcaag agcttcaggg acagtatgac aaagtaagga
     1681 tggatgtcat tgattccaag agcggctcaa cggcaatcgg cctgattgca cttcaggcag
     1741 caaagcttgc aagagcaggg ttgggattcg atatgattgt gagacaaata tgtgagctgg
     1801 ctgatcatgt ggaacatatt ttcatggtac aggatctcag ctggcttatc agaggcggaa
     1861 gaataagcag gaccgaaggg ataattggca gtatactcaa tgtaaagccg attctgcatg
     1921 taaaagacgg ggcggcagaa atcctggaaa aggtcagggg caggaaaaag gcactggatt
     1981 ccattgtaaa tatcatggaa gagaggatta aggattttcc tgaccagaca ataggcataa
     2041 gctatgcatg ggatcaggat attgcgtacg aaataagaga catgataaaa cagaggctgg
     2101 aagttaagaa ctttgtaata aacgaaatcg gagctgccct ggccagccat ttgggaatag
     2161 gaggagttgg ggttttcttt tttaacaggg agccggattt ctatatggaa taaaaggcaa
     2221 aaatatgtta aaatacttat aatctaaata attggaaaag ggaagatatt tatgtctctt
     2281 cgggttggtt ttgacaacaa tttaatatcg caaaacagcg gatatgcttc gggagcatcc
     2341 ggacaagccg ctatgtctcc tgacggcaag aaatccgttg agcaggggca gacaatagac
     2401 agtatccgaa atgacaagct gatgaagaaa attggcgcag tagaatgcca aacctgcagt
     2461 aaccgcaagt atgtcgacgg atctgatgat gccggagtat cctttaagac tcctacccat
     2521 gtgtctcctg aaaactcaat ggcagcggta gcttcccacg agcaggagca tgtttcaaga
     2581 gaaggtgcaa aggcgcgggc agaaggacgg gaggtagtgt cccagtctgt ccgggtattt
     2641 actgatgtgt gtccggaatg cggaagggta tatgcttcag ggggtaagac cactacaatt
     2701 acaaaagccg ataacaagaa taccgattat tttatggata atatgagaaa atccatggga
     2761 atccattttg gaaagtatat tgataaaagg atttaaaact taaacaggtc tccttggggg
     2821 aagagtccga aaagtccccc ggtatgaata aacaggatat ttttataatt tttgaatttg
     2881 cctttcttga tttcctcggt aagcccgtac attgcctttc cggtatatac aggatcaagt
     2941 attattccct ccagctctgc aaagccgtga atgaatctga gttcttccgg acggcttaag
     3001 gcatagcctc ttcccacata accgtctata atatttatat cctcgggaga aaccggaatc
     3061 ttaacatcca tgtactccat gctttcaaac agtatttcag gtattattgc cttgaaatat
     3121 ccggcatctc cgcctacaca aattccgcaa acgtcggaag aatggccgag cagcttatta
     3181 gccagccaca aacctgagta ggtgcctccc gaacctgtgg caagcacaat acaatcaaag
     3241 tgtattccca gctctttttc ctgctcgatt atctcttcca tggcggtgta atagccgaag
     3301 cttcctattc cgttggaagc cccctcaggg attatgtaag ccttgaagcc tttatcatcc
     3361 gtttcccgct ttatggcttc catgatttcc gtcctttgat cgctgtactc ctcagcggtt
     3421 ataaaacgga tatcagctcc cagcagcctg tcaataagga ggttcccctc tgcttcggag
     3481 ccggcatcgc ccctgagaac aaggcaggat ttcattctca gcttggcggc agccgctgcg
     3541 gtagctctgc aatggttaga ttgcacaccc ccgcaggtaa taaggtaatt acagcccata
     3601 tcaagggctt ccttaacaga gtactctaat tttctgactt tattgccgga aagctcggtg
     3661 cctgtctggt catctctttt tatgtatatt tccggccctc caagtacact tgtaagctta
     3721 tccaattttt ctatccgtgt cggcagattt gccaggtgaa ttctttcggg aattttcagc
     3781 atgataagtc ctccattaaa tatttgattt aattatatct catttgctga taaaatgtat
     3841 actgtaatga tatatgctgc gaaataggtt aaaaacttct tgcattatat tgatgtgtat
     3901 ttcgtatcat atgaatcaaa gaaaagtata gtacatatcg tcataataag gcaggttggt
     3961 taaatgagtg atattgcagg aaagggatgt gaaatactgg ttcctttcag tgagaggaaa
     4021 ccgcttcccg aaatagaaag aagccttata aaaaaataca ggaagcacat atggaccagg
     4081 tttatgaaag ctgtgaagga atttgagctt gttaaggagg gggataggat agccgtaggc
     4141 gtatcgggag gaaaggacag tatgcttatg gccaagctca tgcaggaatt acagaggcat
     4201 ggggatgcct cctttgaact tgagttcata gcaatggatc ccggatatca tgagaatata
     4261 agggaattgc ttatagaaaa ctgtaattac ctcgaaatac ccattcatat ttttgaatcc
     4321 ggtatattta aaatagttga taaaattgca aaggattatc cttgctatat gtgtgccaaa
     4381 atgcgccggg gagcacttta cggcaaggca atggaactgg gatgcaacaa gcttgccctg
     4441 ggacatcact tcaacgatgt gatagagact accatgctga acattctgta taccggaagc
     4501 ttcaagacaa tgcttcccaa gctgaagtca acgaatttca aagggctgga gcttataagg
     4561 cctctttact atgtggatga gctttatata gaaaggttta cacaaagcag cggtatctgg
     4621 cctttgaact gcgcctgcat ggtagccgca ggcaagatag ccaacaagcg ctgcgaaata
     4681 aaagctttga taaaggaatt aaaagaaaag atagaaaatg cagatactaa catactaagg
     4741 gccacacaga atgtcaatct ggaagccata ttgggctggc acaagggtgg acagcagtat
     4801 tcctacctgg acttctacga agagcaggag tgatgaaaaa cagcacactt tccggaccgt
     4861 atgaataata tgaattatac tgttctggag gtgtgatggg tgtacaatgc acaaatggcc
     4921 attgcaaata ttaagggcgg tccccttgcg gaacggttga aggggacagt actattcaca
     4981 aatgtacccg gaggagtctg ggtgtgcgta catgtaagcg gcttgtccga ttataaggcg
     5041 gccaaggatg gagggcagcc ggtggggcct cacggttttc acattcatga aaatggcagc
     5101 tgtgaggttg gggatccgga agagcctttc aaggcagccg gaggacattg gaatccggcg
     5161 aatcagcccc atgggaatca tgcgggagat tttccggtgc ttttctccaa caagggtatg
     5221 gcaaggatgt gtttttttac cgataagttc aaaacccggg acataatagg aaaagcagtg
     5281 ataatccatg aaagccctga tgactacaga tcccagcctg cgggcaatgc gggcaagagg
     5341 ctgggctgcg gagtgataag ggaggggcgg taaattatga gctttgttca gaatccggtg
     5401 cgcatatcca caagaaggat tacagctcaa gataatatgg tgactataga ttatccggtt
     5461 gtaaaaggct tggaaagtcc tgctgctcag catagaataa acatgacaat agctgcggca
     5521 gtaaatatgc ttatgaggga gcaaggattt tatgaaaagc agaagaccga gatgctgggc
     5581 tggtatgaga taaaaacaaa tgagagaggt attctgagcc tgacaatagg taattatgcc
     5641 ttcacatatc cctcagctca cgggttaaca atagttaaag ggcttacctt tgatgtgaat
     5701 agcggttaca gcattgaatt aaaggattta ttcaagccgg aagcagatta tgacaaggtg
     5761 ctgtccggga ttgttgagca gcagataacg gacaggaaca tagatctgtt ggatgaattc
     5821 aaaggtataa ggccgtatca ggatttctac attgcggata aatcactggt tctttttttc
     5881 cagttgtatg aaatcacacc ctattacatg ggtttgcagt acttccccat atcggtgtat
     5941 caaattcagg atataatcgc cgaggaagga gcgctgggga ttatggctgc cggagattga
     6001 ccttgtgtta tattaattcc ttttgatata ataatacaaa agaaaaaaga atgaaatgaa
     6061 caagatgata tttggaggta caaaatggaa agcttaagag aacaatttga tatgactgtg
     6121 atatgtgaaa atccggaggc cataaaatga ttaaaagaat agttgcggct ccggattcct
     6181 tcaagggcag ccttacggcc aaggaagcag cagaggccat ggaggcaggc ataaagagat
     6241 atgacaattc aatagaagtt gttaaggtac ctatggctga cgggggagag ggtacagtgc
     6301 agtccatggt agatgcaacg ggagggcaca tgatatccgt aacagtgaag gggcccatgc
     6361 tcagtgaagt agaagcattt tacggcatac tgggagacgg aagtacagct gtgattgaaa
     6421 tggcagcagc ctcagggctc ccacttgtga aacccgagga acggaatcct ttaatagcaa
     6481 caacctacgg aacgggagag cttattaggc atgccctgga tatggggtgc agaagaataa
     6541 taataggaat aggcggaagt gcaacaaatg atgggggagc cggagcaatg caggcgctgg
     6601 gagtaaggct cctggatgac ttcggtaatg atattggctt tgggggagga agtcttggca
     6661 gcctctacag aatagatatc aataaccttg atccaagaat aaaggatact gtgataaccg
     6721 ttgcctgcga cgtagacaac cccctgtgcg gccacaaagg agccagccag gtattcggac
     6781 cccagaaggg tgctgatgat gcgatgattg aggaattgga cagaaacctg gctcattacg
     6841 ccgatttggt ggaaagtact ttgggagtta aaataaagga ctatccgggg gcaggtgctg
     6901 ccggcggatt gggaggaggc cttctggcat ttcttagtgc cacacttaaa aagggtgtgg
     6961 acatagttat tgagactgta gagctggaga aaaaaatcag ggatgctgat ctggttatta
     7021 caggggaagg aaggatggat aatcagacta tatatggaaa gactcccttc ggcgtggcta
     7081 aggcggcgga gaaatacgga atacctgtaa tagcaatatg cggcagtata ggcaagggtg
     7141 ccgaagtact ttatgataac ggctttgcca gtattttttc cataattgac aagcctatgt
     7201 gtattgatga agcgatgaaa aagggcagtg aactgattga gaattgttct gaaagggtta
     7261 taagggcttt gagcataact gagcaataaa ggctgattag ggaggaaagg acatggatag
     7321 tattaatgtg ctgtttgcag cttctgaggc agtacctttt gcaaaaaccg gggggttagc
     7381 agatgttgca ggtactttac cccgggagtt ggtcaagctc ggagctgatg taaggctcat
     7441 tatacctaaa tataaatcca ttccgcggaa ttttgtagaa gcgatggagt atataggcta
     7501 tgtgtatgtg gacctggctt ggaggcatca gtattgtgct gtgctaaagc ttgaatacca
     7561 gggggttatt acatatttta ttgacaatga atactatttc aacagggact gcctgtacgg
     7621 acattttgac gaagccgaac aatttgcgtt cttcagcagg gcagtactca aggtgcttcc
     7681 tctgattgat ttcaaaccgg atattattca ctgcaatgac tggcagacag gtgttgtaag
     7741 cgcatttctc aaagcaggct tcggtcatat tggattcctt aaggatatca agactgtgta
     7801 tacaatacat aatctcaagt atcagggcat gttcccaaag gaagtccttg gcgacatcct
     7861 tggactgagc tgggattatt tcaatcctga aggattggag ttgcacgact gcgtaaatta
     7921 tatgaaagcg ggcatcgcgt attcagatat agtcactaca gtcagcaaga cctatgcaga
     7981 ggagattaaa accgacttct tcggcgagaa tctgaatggc attttaacca aacgctcaga
     8041 gagcattttt ggcattttga acgggataga ctatctggag aacgatccgg agactgatac
     8101 aaggatttat aaaagcttta atgccgaaga tttagaaggg aagtacgaga ataagaagat
     8161 gctgcaggag tctcttgggc ttccgcaaag ggaagaagta cctgttatag gtgtagtgtc
     8221 aaggctggtg gggcagaagg gctttgattt gattgcccat gtacttgatg agatgcttaa
     8281 gatggatatt caattggtgg tgctgggaac gggagacagc aattatgaaa atatgttcag
     8341 aaatgcagct gagagatatc cgggcaaggt ctcagccaac attaaatatg acggcgtgtt
     8401 ggctcaaagg atttatgcgg gttctgacat gttcctcatg ccttcattat atgagccctg
     8461 cggccttagc cagatattca gcttaaggta tggaacggtg ccgatagtcc gggaaacagg
     8521 cgggcttaag gataccgtaa tgccatataa cgaatttaca ggggaaggaa cgggcttttc
     8581 ctttgcaaat tataatgccc atgaaatgct ggatacggtg aagagggcta ttagctttta
     8641 taaaaataag tatatatggg aaaaaatcgt aaaaagcgga atgaagcagg atttctcatg
     8701 gagcaagtcg gcaaaagaat atatggagtt atacgaaaaa atggtttagt aatgcgcaaa
     8761 gctgccaagt ataaagggcc tgaaattttc aagcccttat ttttgacgat atattaaagt
     8821 ccggggatat ttggaagttc actgaagcct ttttgaagat cctcatcggt tgggatatag
     8881 tctgacattt taccgctgtt atactgtata tatgcctgca tatcaaagta acctgttcct
     8941 gtaagcccga agagtattgt cttagcctcg ccggtttccc tgcacttcaa agcctcatct
     9001 atgactgcac gaatagcatg ggaggactct ggtgccggga gtatggtttc ataccttgca
     9061 aaggcagcag ctgcttcaaa tactctggtt tgttcttcag ccctggcttc atttatatag
     9121 ccgtcatgat agagctttga aagcataggg ctcataccat gatatcgcaa acctcctgcg
     9181 tgatttgagg aaggtatgaa tccagagcct aatgtataca tttttgcaag gggagtgatc
     9241 ttgcctgtat cgcagaagtc gtatgcatac ctgcctctgg ttaaggacgg gcaggaggcg
     9301 ggctctactg caataaaaca ggggtttgcc ttgcctgtaa gcttatcctg cataaagggg
     9361 gcaattaggc cgccaaggtt agaacctcct cctgcgcagc cgattatgat atcaggatat
     9421 tcttcgattt tttccatagc aagttttgtt tccaaaccga ttatggattg atgaagcaga
     9481 acctgattga gtacggaacc cagaacgtaa cggcaatttt tcgtatttac cgcatgataa
     9541 atagcttcgg atatagcaca gccaagactt ccgcccgtat cagggttttt ggcaagaata
     9601 gagcgtccgg cttctgtcag attggacggg cttgcaatca cctttgcacc aaaggtttcc
     9661 atgattgatt tacggtaagg tttttgctcc gcagatactt taaccatata tacagtcaga
     9721 ggcataccaa aatatgcaca agcttcagcc aatgccgtac cccactggcc ggcaccggtt
     9781 tctgtggtga gggaggcaat gccttgtttt tttgcgtagt atacctgtgc tattgcagaa
     9841 ttaagcttat ggctgcctga agtattgttg ccctcgaatt tgaaataaat ttttgccgga
     9901 gtatcaagag ccttttccaa gttatatgca cggcaaagag gagaagggcg atagattttg
     9961 tagaattcca aaatctcatc cggaatatcc acatatctgg tttcactatc catttcctgc
    10021 ttcgcaagct cttcgcagaa tacgggatat aggtcattaa tttctgcagg cttcattgta
    10081 ccgggattta gatatggatc atgattttct ttcatatcag cccgaaggtt ataccaatgc
    10141 ctgggaatct ccttttcgga caaatatact ctgtagggaa ttttactcat ttttgcttct
    10201 cctttcttaa tattgattat tgaagtatat aaaaaagctc ttgcccctaa aaatagggac
    10261 aagagcatat actcctgcgg taccacccgt attggtgaaa aatcacccac tcaattcgta
    10321 tacaataata tacgctccat ttataacggg cagagttccc gtcagtaact actcgataaa
    10381 tctttcgtac tgccttcata agtccattca gcaaagcacc tgctgccgca tcacaccaac
    10441 tgcggctctc tgtgagcatt aagctaagct tactattctt aatcatcagt ttttgatatt
    10501 gaaatcaata ttagctctat aatttcaatt tgtcaagaag aaaattgaaa aaacttttaa
    10561 gcccaccagg gagacaggtg ggcttatagc ggttagaaga ccggctgact ataccggtgc
    10621 gccgttgaaa ccgaaggttc ttccaaaacc accaaatgcg ataatcgcaa gaatgatgat
    10681 gaagaagaaa ttgttgtcta taccccaaga tggacctgcc gcaagaagaa tgaacaaaag
    10741 aagcaaaagc aggaacccgt tgttatggca aagatctgca aaaggcgata ctgctttgcc
    10801 ttgtaagcag ccggcagctg ctactgcagg attgactgat ccataactat tagccataat
    10861 tccacctcct ttcagaaata caggttgttt accctttctt caataacata ctatgtacat
    10921 gcataaaaat tgacactata catatcatga aaaaaagcat agaaaatctc aattaaactt
    10981 gatatttctt taacgaagat gtaaaattaa attgtgaatt attgaacaaa gttgattttc
    11041 atagaaattg ggtgatacta tgggtgcaga agttgaatgt atacaaaaaa tgctggagga
    11101 tcacggtata gcgcataccc gtcagagaga agaaatatta agcttgttta ttattgaatc
    11161 aaagcattac aagcctgatg aaattttcaa gcttctcaga ggcaagggga taggcatagc
    11221 tacggtttac aggacactgg agatgctaag ggaatgcggt ataataaaag aaatcagtgt
    11281 aggcaaagaa aggttctacg agcttaagat taatgaaagc aagcatatct acgtgcattt
    11341 taagtgtaac aagtgcggaa agttttttga ctactataat gttgaagcag tgcaggaata
    11401 tgtttccgcg gttgatcagg tagaaaagaa tatgaatgtg aaagtcaatg atgtgaatat
    11461 taacctttat ggaatatgca gccactgcaa gtcttaaaag tctatacgca gccggaggaa
    11521 tgaatttgtt tctccggctg tttttttttg actttttcaa actcttgagg atatggaaaa
    11581 tagcaatccc ataaagcgct gcggcacata gtttcttaat aaaaaggcaa aataatttag
    11641 gtatgaagta tttaaaacta tgcaaggata ggtgaatgcc gtgggaatgg aaatcaacga
    11701 tatggatatg caccagctgg aggatgttct gtacgagaca tcaaaattca atgacaatga
    11761 ttttagtacc tttgaggaag cattgctgga catgtatggg ggtataaaca ctcagaacct
    11821 gaaaagaatc attgattttg ccgagcatga gcttgggata agatctgatt ctgatttgac
    11881 gcatctggac agaaatcaga agaaaaaaat agttgaattt gcttcaacgc tggaataggg
    11941 gagaataaaa catcattctc ctctatttat tattgtatgg gaaagtatac tttcctatac
    12001 aatgggggat gcaaggggga gaagccccct gccgatgaag gagggtgctc catagggttc
    12061 cctagcgaaa aaaaggcgtc gtgagacacg ttttttattt cctatgttcc gcaataagca
    12121 aatctaccat tctgccgtag ctcttgaccc catccttttg attatttgct ttaagatatt
    12181 tgtcattgat tctgtcggtt gcccgctcta ccgggccttc atatccggcc cagaattcat
    12241 ttatcgacgc aaggtcgtga cggacttcat cactgtagct ttcagagagt tttttgaata
    12301 atgcttgatc agtatcatgg agggcattca tggagtttgt caatgcaagc aatatgccgg
    12361 aatacctgta gttgacatca gggtgggcac tacaggccag ataggaaata taattggcct
    12421 cgtcctctct ggaaaatccc ctttgatggg ccatttcatg ggatgcggta tagggcagtg
    12481 ttgactcagg tattgcaata ttcacattag cttctccggt aaaagggaag tacacgccgc
    12541 agaggcccgt ataattcata agacgggata acagaacagg tttggggtgg ctgtaactgc
    12601 cgcttaactc cggatacttg ccggcaaccg attcataacc cttatggcag tttttcagta
    12661 tatcacgggg gctgccggga atagccacat ttccttccga atccacactt actgacaatc
    12721 ttagggcatt tgccctttca atcaaatcca ggcacaggct ttccagttca tagaccgatg
    12781 ccggacgtac ctccaggcct gcaatacttg caaaaggcat tctgtaataa ttcaatcccc
    12841 aaacgcctat gaagctgaaa aagactatac tgactattac aagaatattg gtcaggtatt
    12901 ttataagctg catgtttata agggccctta cagtataaac aataaagacg gcaggaacaa
    12961 gtattactat gaactcggca gcagagaagg gcagtattcc gaaagctttt gaaaggatgc
    13021 ttcccaaggg cttataaatt ccattggaat acagcctttc aacaagttcc ggattccttg
    13081 aagcgaaata gacaaggagc attccaaggg gtataagaat agtaagctta agccttttta
    13141 atattttttt gttcctttta ttccacatat agaaatcagc ctttacttca tttaattgca
    13201 catataaccg tatatattat aacattttgt acacag
//
LOCUS       DAOQUR010000060        12998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5406_length_12998_cov_10.869041, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000060 DAOQUR010000000
VERSION     DAOQUR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5406_length_12998_cov_10.869041"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PKI50_10290"
     CDS             complement(<1..1241)
                     /locus_tag="PKI50_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04129.1"
                     /translation="MAGPFSRRPAAKPKDTGKTLKRIMKYLFAYKLQLAAVAVSIIVS
                     AAASVAGTYFLKPIINQYIVPYIGSKNPDMTGFASMIGMLAAVYALGVAGTYAYNRLM
                     LNISTGTLYKLRTELFAHMESLPIKYFDTHTHGELMSRYTNDIDTLREMLSHGIPNLF
                     SSALTVIGIFAMMVVLSPALTVLVVFVLILILFVMKKIGGLSAKYFRKQQKEIGRVNG
                     YIEEMIEGQKVVKVFSREQIVKSDFDKINDELFHAAANAHTFAGILMPIIGNLSYMLY
                     SLTAAVGAIMSIKGYIDIGTIASFLQYTRTFTQPLNQISQQMSAILTALAGAERIFEV
                     LDEKPEVDEGIVELVHARKKDRGELEEAEKYTGMWAWRQPNEDGSINYSELKGDVRFE
                     DVTFGYEENKTVLDGISLYAKP"
     gene            complement(1245..2972)
                     /locus_tag="PKI50_10295"
     CDS             complement(1245..2972)
                     /locus_tag="PKI50_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04130.1"
                     /translation="MLKKLLPCLGSYKKYAVLTPVMVIGEVILEIFIPLIMSKIIDVG
                     IGKGDIGYVVRMGALMVLMALFALLFGVLSGRFAAVASMGFAKGIRKTLFDKIQEFSF
                     ANIDKFSTASLITRLTTDINSVQMAFMMIIRMLVRAPIMLVSATVMAIVINARLAVMF
                     LAAIAFLGAALYFIAIRAYPRFKAMLERYDSMNASVQECLIAIRVVKAFVREDHENEK
                     FRAAADAVRKAQLKAEKMLILNMPIMQFVMYCCMIAISWFGGRMIIGGTLLTGEFMSF
                     ISYVGQIMMSLMMISFVFVVVVMSRASAFRIAEVLDEEIDIRDSSGNKDLKVEDGSVV
                     FDSVSFSYSKNKDNLTLNDCSFEIKPGETVGIIGGTGSGKTSLVQLIPRLYDVIEGRV
                     LVGGHDVRDYKLGILRNAVAVVLQKNVLFSGTIKENLRWGNVEATDSEIIEACKTAQA
                     HDFIASFPEGYDTYLGQGGVNVSGGQKQRLSIARALLKRPKILILDDSTSAVDTATDS
                     KIRQALRENLKDTTKIIIAQRITSVSDADRIIVMDDGRINAIGTHDELLAANSIYREV
                     YESQQKGVA"
     gene            complement(3506..3595)
                     /locus_tag="PKI50_10300"
     tRNA            complement(3506..3595)
                     /locus_tag="PKI50_10300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3559..3561),aa:Ser,seq:cga)
     gene            complement(3599..4027)
                     /gene="tadA"
                     /locus_tag="PKI50_10305"
     CDS             complement(3599..4027)
                     /gene="tadA"
                     /locus_tag="PKI50_10305"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNT04131.1"
                     /translation="MRKALEEAEKAFDKDEVPVGAVIVRKGEIIAAAHNCREILKDAT
                     AHAEILAIKEACEALGGWRLIDCDMYVTLEPCVMCSGAILQARIERLYIGAMDPKGGA
                     AGSTLNLFEDYHFNHRTEVIKGILEEECSQILKDFFKRKR"
     gene            complement(4221..4937)
                     /locus_tag="PKI50_10310"
     CDS             complement(4221..4937)
                     /locus_tag="PKI50_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04132.1"
                     /translation="MSKVVLSLKNLATRYGQIYALKGISLDVCEGEIVTLLGSNGAGK
                     STTLKTISSLVPACSGKVEFYDKDITNAPPHDIVEMGIIHVPEGRRIFKELNVQENLE
                     MGSFTLKDDAERKRRMDRALELFPVLKERRKQMGGLLSGGEQQMLAIARALMTDPKVL
                     LLDEPSMGLAPIIVQGIMDIIKQINEQGTTILLVEQNAKAALKLAHRGYVLETGEIVM
                     EGNADVLSKDDSIVKAYLGH"
     gene            complement(4934..5707)
                     /locus_tag="PKI50_10315"
     CDS             complement(4934..5707)
                     /locus_tag="PKI50_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04133.1"
                     /translation="MLILEVKDLTLRFGGLTAVNKVSFGIDEGSIESLIGPNGAGKTS
                     LFNCLTGFYKPQEGDVLFQGKSIMKKKPHKITALGMARTFQNLRLFKNLTVMENVMSG
                     MHCRGSAGVAGAILKPPSQRAEEKRIREVALECMDFIGILDKKDRLAKNLPYGDQRRV
                     EWARALATQPKLLLLDEPAAGLNYDEKMQLIELIRRIRDERKMTVFLIEHDMGLIMKV
                     SEKIFVIDYGVKIAEGNVQEVQNNPKVIEAYLGKEEDAE"
     gene            complement(5750..6754)
                     /locus_tag="PKI50_10320"
     CDS             complement(5750..6754)
                     /locus_tag="PKI50_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT04134.1"
                     /translation="MKEKQTAAARTAIKRDNPLAGLINRLYIIFDNKKIRYSVLAIAV
                     AVLLYLPMITSLYFMEVMTNVWFNVTVCLGLNIIVGFAGLLSLGYAAFFAVGAYTTGI
                     LISHYGVSFWLTLPVSVLMAIIAGLAIGAPTLKLRSDYLAIVTLGFGEIIRLAARNLQ
                     ITGRATGLVGIDRPVLFGMTLYKINHFYYAFLLVAILAIFVSYRLQDSRLGRAWKCIR
                     EDEDAAEAMGINVVAVRLYAFVIGSVFGAVAGSFMAVKMTAISPESFLFTQSVMILLA
                     VVLGGMGKIPGVILGAFAIVVFPEVFRGIGEYRMLIFGLLLLVLMIYRPQGLWPERHT
                     "
     gene            complement(6760..7677)
                     /locus_tag="PKI50_10325"
     CDS             complement(6760..7677)
                     /locus_tag="PKI50_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT04135.1"
                     /translation="MSFREIIIQQLFNGLLLGSFYGLVALGYSMVYGIIKLLNFAHAD
                     IYMVGGFCSLSLLAFFGGTFFGNGWLGIIAAMLITMIIVGLLGVLIERIAYRPLRDAP
                     RLSLLITALGVSMILYNSVMVITGGEYKAYKTGLGFEGFSLGSVHVTYTQAILIVAAV
                     SLMFVLHLFITRTMYGKAMRAISMDLDCCRLMGVNVNKIIALTFFVGSALAASAGMMA
                     GVYYGSLHFFMGFVIGIKAFTSAVIGGIGVIPGAMLGGVLLGLLESFGTQIPFIGSEW
                     KDVFSFSILILILIFKPTGLLGKKESERM"
     gene            complement(7750..8907)
                     /locus_tag="PKI50_10330"
     CDS             complement(7750..8907)
                     /locus_tag="PKI50_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT04136.1"
                     /translation="MVKRLMILTLVVVLMASVVGCAKPAEPPADTGDASAPKFDGELL
                     LGVMVPVTGSEATYGKDMENAIKIAVEEINAAGGLLGKEVKLTLGDDGCDPQMGSAAA
                     SKLVSAEVKAVLGGYCSGATIPTLKIYGDAKVPFVIIAANSTKIIEENPGWAFMINSP
                     GYHQANAAADCHEKLGTTKLALVHQGDGFSEDLATLTKKEWESRGKQVVGFDVVNKGE
                     QDFSSLVTKIKSSGAEGVYWTAYHADGALLIKQLRQGGFTGNIVVADGSSSVQLLEIA
                     GQAGEGVYCTSPPVVDFLPAAKEFISKYTAAYNQQPGPYSGLAYDGTHLLADAIKRAG
                     STDPDAIRSALEDTKDFKTLSGTITFTPEKVLEKSNFISIKGVGGKWVLAE"
     gene            9596..11992
                     /locus_tag="PKI50_10335"
     CDS             9596..11992
                     /locus_tag="PKI50_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HNT04137.1"
                     /translation="MFQTTHEPKYMERIIRLKNRVLETRPEMDLENASILTEGFREAE
                     GEPLVVRKAKAFRKQCLEKTVTIWDDELIVGCSGSKTRAGILCADSCWSVLNDELETI
                     SERRYDPFYLKPEDRKIFEEEIRPYWKGRSNYEEWLAQIPDDTRALRDNGVVYIDRKA
                     VRGWGETTAGYEWMIREGVSGIIKVVEKRLSELDITIPGDYEKQYYLKALLIAADGII
                     KLAERYADEADRLAAAKKDPQRKKELLEIAQVCRHVPANPARSFREAIQSFYFYHICI
                     FMEQNAASYNPGRMDQYLWPYYKADIEAGRISPEEAQELLDCLWVKFSEPCLFQDAVT
                     AEFAAGYPMFQNVCVGGVDDTGRDAVNDLSYMILQATMDVQLYQPSLSVRYNLAKNPN
                     AFLRKVVELISLGTGFPAFHNDDVGIMMLMNKGIPLREAFNWNPCGCVETNLEGRLRQ
                     YTALADINLGSMVEFALLDGKNRKSGRYISERTGNPTNFLTYEEFLSAVKKQISYAIR
                     AVVKGSHVIDEIGKNRPCPALSLTFKECIEKAKDYAWGGAKYNTGNGIIMIGVADLIN
                     SIAAVRHIVYGTKQATMKKLLEALDNDFEGYEELHKACLDAPKYGNDDSMVDDIAGDM
                     FTFIADEIEKYSSKMGRMTPGILPVSGNTPFGLEVGALPSGRKAWKPLADGVSPSGGT
                     DFNGPGSVLKSVANIPHGRFVQGTLLNMKVEPAMLATENGIAQMMALLKSMCSLGVYH
                     VQFNVIDQEKLIHAQKNPEEYKGLLVRVAGYTAYFVELGKDVQDEIIGRTVQHRISAG
                     "
     gene            11998..12945
                     /locus_tag="PKI50_10340"
     CDS             11998..12945
                     /locus_tag="PKI50_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNT04138.1"
                     /translation="MNNNTSVIGSVLRIEKASIHDGKGLRTVLFLKGCPLRCRWCSTP
                     ESQSFGLQKGYARDRCIGCGICVRSCPERALSISHDTQKVLTDLSKCKKCFICAAKCP
                     RRAIKQYGSLMSVKEVIGEISKDEIFFFHSGGGVTISGGEPLNQPEFVSEVLRECRAL
                     GIHTAIETSFHVSYANVEKTLPWLNVLYVDLKHMDKDLHKDWVGHENSLILDNIKKAD
                     QSEYPLEIIVRIPMIPGVTDTDTNLSAVAEFCKSIKKLKEIELLPYHRLGTDTYKHLG
                     MEYLLKDLAPPSQEHISERARFLTELNPGVPVRIGSGLG"
BASE COUNT         3712 a         3104 c         2579 g         3603 t
ORIGIN      
        1 ggctttgcat agaggcttat tccgtccaat acagttttgt tctcctcata gccaaaggtg
       61 acatcttcaa agcgcacatc ccctttaagt tcactgtagt ttatgcttcc atcctcgtta
      121 ggctgcctcc atgcccacat tccggtatat ttctcagcct cctccaattc acccctatct
      181 tttttccttg catggactag ctcaactatg ccttcatcca cctccggctt ttcgtccagc
      241 acttcaaaaa tgcgctctgc acctgccaaa gcggtcagta tcgcgctcat ctgctgcgaa
      301 atctggttca gcggctgtgt gaacgttcga gtatactgca aaaatgatgc aatggtccct
      361 atgtcaatat agccctttat agacataatt gcacccaccg ctgcagtcaa tgaatacaac
      421 atataagaaa ggtttccgat aatcggcatc agtattcctg caaaggtatg ggcgtttgca
      481 gccgcatgga acagctcatc gtttatttta tcaaaatcac tctttacaat ctgctctcga
      541 ctgaatacct ttacaacctt ctgcccctca atcatttctt cgatgtaacc gttaaccctg
      601 ccgatttcct tttgctgctt cctgaaatat ttagcactca aaccgcctat cttcttcatg
      661 acaaacagaa tcagtatgag cacaaacact acaagaacag tcaatgcagg gctgagcaca
      721 accatcatgg caaatatccc aatcacagtc aatgctgatg agaacaggtt cggaatacca
      781 tgactaagca tttccctaag agtatcgata tcgtttgtat accggctcat caattcacca
      841 tgggtatgag tatcgaaata ttttatcggc aaggattcca tatgagcaaa taattctgtt
      901 ctcaatttat acaaagtgcc tgtggaaata ttcagcatca gcctgttata tgcataggtt
      961 cctgcaacac caagggcata tacggcagcc aacatgccta tcatggaagc aaatcctgtc
     1021 atatcaggat ttttactccc gatatatgga acaatatatt gattaattat tggcttaagg
     1081 aaatacgtac cggcgacgct tgctgccgca cttacaataa ttgaaactgc cacggcagca
     1141 agctgcagct tatatgcaaa taaatatttc ataatccttt ttagtgtctt tcccgtatct
     1201 ttcggctttg cagccggacg acggctgaaa ggtcccgcca tggcttatgc aactcctttc
     1261 tgctgtgatt catatacctc tctgtaaatg gagttggcgg caagcaattc atcatgggta
     1321 ccaattgcgt taatcctgcc gtcatccatt actataattc tatcagcatc actgacggaa
     1381 gttatcctct gagctataat tatttttgtg gtatccttca ggttttccct gagtgcctgg
     1441 cggatcttac tgtcggtagc agtatcaaca gcacttgtgc tgtcatccag gatcagtatt
     1501 ttaggccttt taagcagcgc ccttgcaata gacaacctct gcttctggcc gccggataca
     1561 tttacgcctc cctgccccaa gtaagtatcg tagccttcag gaaaggaagc tataaaatca
     1621 tgtgcctgtg ctgttttaca ggcctcaata atttcagaat cagttgcctc tacatttccc
     1681 catctgaggt tttcctttat agtccccgaa aagagaacat tcttctgcaa taccacagca
     1741 actgcgtttc tcagtatacc aagtttataa tcacgcacat cgtgccctcc gactaaaacc
     1801 cttccttcaa ttacatcata gaggcggggt ataagctgca caagactggt tttgccggaa
     1861 ccggtacctc ctatgattcc tactgtctca ccgggtttta tttcaaagct gcagtcattc
     1921 agggtaagat tatctttgtt tttactgtag ctgaaggaaa cactatcaaa aacaactgag
     1981 ccgtcctcga ctttcaaatc cttgtttccg ctgctgtccc tgatgtcaat ctcttcatcc
     2041 agaacctcag ctatacggaa tgcagatgcc cttgacatga caacaactac aaacacaaat
     2101 gagatcatca tcaatgacat cattatctgt cccacatagc tgataaaact catgaattct
     2161 ccggtaagaa gcgtccctcc gattatcatc ctgcccccga accacgaaat ggctatcatg
     2221 cagcagtaca ttacaaattg cattatcggc atatttaata taagcatttt ttccgccttc
     2281 agctgtgctt ttctaacagc atctgcagcc gccctgaatt tttcattttc atgatcttct
     2341 cttacgaagg cttttacaac acgaattgca atcagacact cctgaacgga agcattcatg
     2401 ctgtcatatc tctcaagcat agccttgaat ctgggatatg ctcttatggc gataaaatac
     2461 agcgcagcac ccaaaaaagc tatggctgca agaaacataa cagccagacg ggcatttatt
     2521 acaattgcca tgacagttgc actaacaagc attataggcg cacgcacaag catccttatt
     2581 atcatcatga atgccatctg cacactgttt atatcagtgg taagccttgt gataagagat
     2641 gctgtgctga atttatcaat gttggcaaag gagaactcct gtatcttgtc aaacagcgtc
     2701 ttacggatac cttttgcaaa acccatactt gcaacagctg cgaaacgccc tgacaggact
     2761 ccgaataaaa gtgcaaacaa agccataagg accatcaaag cccccattct gacaacatag
     2821 cctatgtcac ccttgcctat acccacatca attatcttcg acataataag aggtatgaat
     2881 atttccagta taacctcccc tattaccatc acaggagtta aaaccgcata tttcttatag
     2941 ctccccaagc agggcagcag ctttttcagc atattattcc tccttgaatt gcttaatgcc
     3001 catgtcaaag agaattataa cataacgcat cgttgagtgc cagtttatca cctatcctat
     3061 ttccctggtt tatgaaataa ttcaagggtt ttcagacaga taataacgga gagggaggga
     3121 tttgccgcct gcgacccttc gctcgcatgc tggtgacgtc gcccaccctc gcatccgctc
     3181 cggtaccggg ctcctacaat ttgctaaaaa tgcccgcaca ggagcatttt cttaacgcaa
     3241 attgccctca cgggttcgaa tccctccaaa atttcttcat ttaaagaaaa ataaaaccgc
     3301 ccctgcggga cggttatttt tctttaatgg cggagaggga gggattcgaa gccggacggc
     3361 cggcagccaa actttatttg tgcattacga taactattta aacagccact tcagtaattc
     3421 ttatcttctc tggcctcatg agggttctca ccatcccctc tgcagataaa aaataaaacc
     3481 gctttcagcg gtttgatttt ttatctggcg gagagggagg gattcgaacc ctcgagggct
     3541 ttgacaccct gcatgatttc gagtcatgga ccttcgacca ctcggacacc tctccgtgtt
     3601 atctctttct tttaaaaaaa tccttcagaa tttggctgca ttcttcttcc agaatgccct
     3661 ttattacctc ggtcctgtgg ttaaaatgat aatcttcaaa taggttcagc gttgagcctg
     3721 ctgcaccgcc tttcggatcc atggcgccta tataaagcct ttctattctg gcctgcaata
     3781 tggcgccgct gcacataacg cagggctcca aagtcacata catgtcacag tcaataagcc
     3841 gccagcctcc gagtgcttca caggcctctt ttattgcaag tatttctgca tgagccgttg
     3901 catcttttaa aatctctctg cagttatgcg ctgctgctat tatctcacct ttccttacta
     3961 tgacagcacc aaccggaacc tcatccttgt caaaagcctt ctctgcttct tccaaagctt
     4021 ttctcataaa aagagcatcc aatttctacc acgccaattc ataattaata tatattatta
     4081 tgcggtttac cttgatatat tatcatttaa gacgttgaat tgtcaatgag ctgccataaa
     4141 tttacaaccg cagcaaaaaa ctaataataa agattaaaaa atcccaatac ttcatttgta
     4201 ttgggattta atactttatt ttaatggccc aggtaagcct tcactatact gtcatctttg
     4261 cttaatacgt cagcattccc ttccatcact atttctcccg tttccaacac ataccctctg
     4321 tgggcaagct taagggcagc ctttgcgttt tgttcaacaa gcaggattgt agtgccttgt
     4381 tcattaatct gtttgataat gtccattatc ccttgaacaa ttatgggtgc caaccccatt
     4441 gagggctcat ccagcaaaag caccttaggg tcagtcatga gagcccgcgc tatagccagc
     4501 atctgctgct caccccctga gagaagccct cccatttgct tacgcctttc ttttaataca
     4561 gggaacagtt ccagtgccct atccattctt cgctttcttt cggcatcatc cttaagtgta
     4621 aatgaaccca tttccaagtt ttcctgaacg ttcagctcct taaaaatcct tcgcccttca
     4681 gggacatgaa ttatacccat ctcaacaata tcatggggcg gcgcatttgt aatatctttg
     4741 tcgtagaatt ccacctttcc ggaacatgcc ggcaccaaac tggatatagt tttaagagtg
     4801 gtgctttttc ctgcaccgtt ggagcctaaa agcgtaacta tctccccttc gcagacatct
     4861 aggctgatac cctttaatgc gtatatctgt ccgtatcggg ttgccaggtt tttcagggaa
     4921 aggactactt tactcattcg gcatcctcct ccttccccaa atatgcctct attaccttgg
     4981 ggttgttttg aacttcctgc acattgccct cggcaatttt gactccgtag tcaattacaa
     5041 atattttttc cgagaccttc ataatcaagc ccatatcatg ttctattaaa aaaacggtca
     5101 tttttctttc atcccgaata cggcgtatta gctctatcaa ctgcattttt tcatcatagt
     5161 tcagtccggc ggcaggttcg tccagcagca gcagctttgg ctgagttgcc agtgcccttg
     5221 cccactctac ccttctttga tccccatagg gtaaattctt tgccaatcta tctttcttgt
     5281 ccaaaatgcc gataaaatcc atacattcta aagccacttc cctgattctt ttttcttccg
     5341 ctctttgtga aggaggcttt aaaatggctc ccgctacacc tgcgctgccg cggcagtgca
     5401 ttccggacat gacattttcc atgacagtta agttcttaaa cagcctaagg ttttggaaag
     5461 tcctggccat ccccaaagct gtgatcttat gcggtttctt cttcataatg cttttgccct
     5521 ggaacaggac atccccctcc tggggcttgt agaaaccggt caggcagttg aaaagcgatg
     5581 ttttcccggc accgttaggt cctatcaggc tttcaataga gccttcgtca atcccaaagc
     5641 tcaccttatt gactgctgta agtcctccaa atcttaatgt taagtctttc acctctaaaa
     5701 tcaacattgt tcaccccgtc cgatataaat tttttccccg cagtaaatat caagtatggc
     5761 gttcaggcca caggccctga ggacgataaa tcattaaaac taataatagc agaccaaata
     5821 ttaacatcct atactcgcca atccccctga aaacctcggg gaaaactact attgcaaaag
     5881 cccccagtat tacgcccgga atttttccca tacccccaag tactaccgcc agtaaaatca
     5941 taactgactg ggtaaacaaa aaggactcag gtgatattgc cgtcattttt actgccatga
     6001 aagagccggc tacggctccg aagacagagc caattacaaa agcataaagt ctgaccgcaa
     6061 caacattaat acccattgct tctgcagcat cctcgtcctc cctgatacac ttccatgctc
     6121 ttcccagtct cgaatcctgc agtctgtacg atacaaatat ggctaggata gccactaata
     6181 aaaatgcgta ataaaaatgg tttatcttat acaaggtcat accaaacagg acaggcctat
     6241 ctataccgac cagcccggtg gcacgtccgg ttatctgcaa atttctggct gccagcctga
     6301 taatttcacc aaaacccaga gtaactatgg ccaaataatc gctcctcagc ttcaaggttg
     6361 gtgctccaat tgccagcccg gcaataattg ccataagaac actgaccgga agagtaagcc
     6421 agaagctgac tccataatgt gaaatcagta ttcccgtggt ataagcgccc actgcaaaaa
     6481 atgctgcata gccaaggctg agcagccccg caaacccaac tattatatta aggccaagac
     6541 atacagtcac gttaaaccaa acgttggtca taacctccat aaaatacaaa ctggtaatca
     6601 taggcaaata aagcagcaca gctacggcaa tcgccaaaac gctgtaacgt atttttttat
     6661 tatcaaaaat tatatacagt ctatttataa gtcctgccaa ggggttatca cgttttatag
     6721 ctgtccgagc agctgcagtc tgtttttcct tcatagactc tacatcctct cgctttcttt
     6781 tttacccaaa agtcctgttg gtttaaaaat cagtataaga ataagtatcg aaaaggaaaa
     6841 tacatccttc cattctgagc cgataaaagg aatttgtgtc ccaaaggatt ccaataagcc
     6901 cagcaatact ccgcccagca tagcgccggg aattacccca atccctccga taacagcgga
     6961 tgtaaaggct tttatgccta taacaaagcc cataaagaaa tgcaggcttc cataatacac
     7021 tcctgccatc attcccgcag aagcagccag tgctgaaccg acaaaaaatg tcagggcaat
     7081 gattttattt acgttaacgc ccataagacg gcagcaatca agatccatgg atattgccct
     7141 catcgccttg ccatacatcg ttctggtaat aaacaaatga aggacaaaca tcaatgaaac
     7201 ggccgctact atcaatatag cctgagtgta tgtaacatgc acacttccaa gggaaaagcc
     7261 ttcaaaaccc aatccggtct tataggcttt atattcgccc ccggttataa ccattaccga
     7321 attatacaaa atcattgaaa cgcccaatgc ggtaataaga agggaaagtc gcggagcatc
     7381 acgcaaaggc ctataagcaa ttctttctat cagaacgccc aataaaccaa caattatcat
     7441 agtaatcaac attgctgcaa ttattccaag ccaaccgttg ccaaaaaagg ttcctccaaa
     7501 gaaagccaat aaggataaac tgcaaaatcc acccaccatg tatatatctg catgggcaaa
     7561 gttcaacagt ttaattattc cataaaccat agaataccca agtgcgacca atccgtaaaa
     7621 ggagccaaga agcaagccgt taaacaactg ctgaattatt atttctcgaa aagacataag
     7681 cccacccttt catttatgaa agtatgcgca ggccaggagt aatatgatac ctgacctgcg
     7741 ccaccatgtt tactcagcca gaacccattt gccgcctaca ccttttatac ttataaagtt
     7801 gctcttttcc aagactttct caggtgtaaa ggtgattgtc ccagaaaggg ttttaaaatc
     7861 tttagtatct tccaatgcgc ttcggatagc atccggatcg gtgcttccgg ctctttttat
     7921 agcatctgcc agaaggtgtg taccatcgta ggcaagccct gaataaggac ccggctgctg
     7981 attatatgca gcagtatact ttgaaatgaa ttcttttgct gccggtaaga agtctacaac
     8041 cggaggcgat gtgcaatata caccttctcc cgcctgtccg gcaatctcca ataactgtac
     8101 agagctggaa ccgtcggcta caacaatatt acctgtaaat ccgccttggc gcagctgctt
     8161 tatcaacagc gctccgtcag catgataagc tgtccagtat actccttcgg caccggagga
     8221 cttaatcttg gtaaccaagg atgagaaatc ctgttctccc ttatttacta cgtcaaagcc
     8281 cacaacctgt ttgcctctgc tctcccattc cttttttgtc agggttgcca aatcttcgga
     8341 aaaaccgtct ccctgatgaa caagggcaag tttggtagtt ccgagttttt catggcagtc
     8401 agcagcagca tttgcctgat gatacccggg gctgttgatc atgaatgccc agcccggatt
     8461 ctcttcaatt atctttgtag agttggccgc tataataaca aagggcacct tggcatctcc
     8521 ataaatcttc aatgtaggta ttgttgcacc ggaacagtat cctccaagta cagccttcac
     8581 ttcagccgat accagtttgc tggcagcggc gctgcccatc tgaggatcac agccgtcatc
     8641 tcctaatgtc agttttacct ccttgcctaa caggccgcct gcagcattaa tctcctccac
     8701 tgcaatttta atagcgttct ccatatcctt accataagta gcttctgacc cggtaacagg
     8761 taccatcaca ccaagcagca gttcaccatc aaacttaggt gcagatgcat ctcctgtatc
     8821 tgccggcggc tcagccggtt tcgcgcaacc gaccaccgat gccataagaa caactacaag
     8881 cgttagaatc attagtcttt ttaccatcaa atttccctcc cctaattaat ttatcacatg
     8941 ggttcttgcc caaagggttt aaacccaata aattggacaa atctatcagc aggctcttcc
     9001 aggccccggt acagttaacg aagagatatc actaatatat aatcatctga gtttaatctg
     9061 attttataac ttgcacaaaa aaagctttga cggtataagg tatgatatta cttagtatgg
     9121 tataattatg tataatagta ataaattagt gcatatgcat atggcagact gctaatatga
     9181 attaacgtat tcgtttaaaa aagagagctg cattcaatag ttaataccga caagcacgaa
     9241 ggaatcatat atttcacgcg gtacactcac tgatatcgga actgctgtta tttccgcata
     9301 aatacatgca tattgtagga actgtttaca ctcttgtaaa gaaatatatc ataaattcta
     9361 gttattgtaa agactgtttt ctgaacatac ggaatttgca gaatttacgt taaattgaag
     9421 cattaatcgc agaatgctgt caaggataaa aaaaattgta taaatatgca aaaaagattc
     9481 tgagtgacaa aaaatgctcc gaatgatatg cttgatttag atagcggcat ataaatcgtg
     9541 tgtcaacaag tgaagtaatg atatctctca tatgattttt aacggaggtg tctgtatgtt
     9601 ccaaacaaca catgaaccaa aatatatgga gcgaataata cgactgaaaa acagagtgct
     9661 ggagactcgc ccggagatgg atcttgagaa tgcaagtata cttaccgagg gttttcgcga
     9721 ggccgaaggg gaaccccttg ttgtcaggaa agccaaggct tttcgtaaac agtgccttga
     9781 aaagacggtt accatatggg atgatgaact gattgtgggc tgctcgggaa gcaagaccag
     9841 ggcgggtatt ttatgcgctg actcttgttg gtcagtactg aatgatgaac tggagaccat
     9901 cagcgagcgc cgttatgacc ccttttattt aaaacctgaa gatcgaaaga tatttgaaga
     9961 agagatacgc ccttattgga aaggcagatc caattatgaa gaatggcttg cacagattcc
    10021 tgacgatacg cgtgctcttc gggataatgg agtggtgtat atagaccgta aggctgttcg
    10081 cggatgggga gaaaccacag ccggatatga gtggatgatc cgtgaaggag tctcgggaat
    10141 catcaaagtg gtggaaaaac ggctgtcaga gcttgatatc actatccccg gcgattatga
    10201 aaaacaatac tatttaaaag ctcttttaat tgcggctgac ggtataatca aattagcaga
    10261 gagatatgcc gatgaagctg accgtttggc tgctgcaaaa aaggatccgc agaggaaaaa
    10321 ggaacttttg gaaatagcac aagtttgccg tcatgttccc gcaaaccctg ccaggtcatt
    10381 cagggaagca atacagtcat tttatttcta tcatatctgt attttcatgg agcagaatgc
    10441 tgcaagctat aatccgggac gtatggatca atatctatgg ccttactata aggctgatat
    10501 tgaagcaggg cgcatctcac ctgaggaggc tcaggaactg ttggactgcc tttgggtgaa
    10561 gttcagtgag ccgtgtcttt ttcaggatgc agtgaccgca gagtttgctg ccggttatcc
    10621 tatgtttcag aatgtatgtg tcggaggggt agatgacacc gggagagatg ctgttaacga
    10681 tctttcctat atgatcctcc aggctaccat ggatgtacag ctctatcaac catctctgtc
    10741 agttcgctat aacctggcta aaaaccccaa tgctttttta agaaaagtag tggagttaat
    10801 cagtcttgga acaggctttc cggctttcca taatgatgat gtgggaatta tgatgctcat
    10861 gaacaagggc attccattaa gagaagcctt caactggaac ccctgcggct gcgtggaaac
    10921 caacctggag ggacgtttgc gccagtatac ggcacttgct gatattaatc ttgggagcat
    10981 ggttgagttt gcactattgg acggaaagaa caggaaaagc ggcaggtata tttccgaaag
    11041 aacaggtaat cccacaaatt ttttaacata tgaagagttt ctctcagcag ttaaaaagca
    11101 aatttcttat gcaatcagag ctgttgtgaa gggaagccat gtcattgacg agattggaaa
    11161 gaaccgccct tgccctgccc tttcattgac cttcaaggag tgcattgaga aggcaaagga
    11221 ctacgcctgg ggcggagcga aatataatac cggaaatggc attattatga ttggcgtagc
    11281 agacctaatc aatagtattg ctgcagttcg gcatattgtt tacgggacga agcaggcaac
    11341 catgaagaaa ctgttagagg cactggacaa tgattttgag ggttacgaag agcttcacaa
    11401 ggcatgcctt gatgcaccaa agtatggcaa cgatgattcc atggtggatg atattgccgg
    11461 agatatgttt acttttattg cagacgaaat tgaaaaatac agcagtaaaa tggggcgaat
    11521 gacccccggc attttaccgg tatccggaaa tactcccttt ggccttgagg ttggtgcttt
    11581 accatccggc cgtaaagcat ggaaaccatt ggcagatggt gttagtccca gcggaggaac
    11641 cgacttcaac ggtcccggtt cagttctgaa gtcggtagcc aatattcctc acggccgttt
    11701 tgttcaagga acattgctga atatgaaagt agaaccggcc atgctggcta ccgaaaacgg
    11761 aatagcgcag atgatggctt tgctgaagag catgtgcagc cttggtgttt atcatgttca
    11821 gttcaatgtg atagatcagg agaaattaat tcatgcccag aaaaatcctg aagaatataa
    11881 agggcttctt gtaagggttg cgggttatac tgcttatttt gtggagcttg ggaaagatgt
    11941 ccaggatgaa ataatagggc gcacggtaca acaccgcatt tcagcggggt gattttaatg
    12001 aataataata catcggtaat tggttctgtt cttagaatag aaaaggcatc aattcatgac
    12061 gggaaagggc ttcgaactgt gctctttcta aaagggtgcc ctcttagatg cagatggtgt
    12121 tctactcccg aatcccaaag cttcggactc caaaaaggct atgcccgaga ccgctgcata
    12181 ggatgcggaa tatgtgtgcg ttcgtgcccc gagagggcac tttctatatc acatgatact
    12241 caaaaggtgc tgactgattt atcaaaatgc aaaaaatgct ttatctgcgc tgcgaaatgc
    12301 ccccggagag caatcaagca atatggaagc cttatgtcgg taaaagaagt tataggagaa
    12361 atatccaagg atgagatttt ctttttccat tcaggaggcg gagtaacaat aagcggtggt
    12421 gagcccctga accagcctga atttgtctct gaagttctcc gggagtgcag ggcactgggc
    12481 attcatactg ccattgaaac cagcttccat gtctcttatg cgaatgtgga aaaaactctg
    12541 ccgtggctca atgttcttta tgttgatctt aaacatatgg ataaggatct tcataaagat
    12601 tgggttgggc atgagaattc attaatcctg gataatatca aaaaagcgga tcaatccgaa
    12661 tatccattgg agataatagt acgcattccc atgataccgg gggtaaccga taccgacacc
    12721 aacctgtcgg cagttgcgga attctgcaaa tccatcaaaa aacttaaaga aatagagtta
    12781 ctgccttacc atcgccttgg tactgatact tataagcatc tgggaatgga atatttactg
    12841 aaagatcttg caccgccgtc acaagagcac atatctgaac gggcaaggtt cctgactgag
    12901 ctgaatccgg gagttccggt aagaatcggc agcgggttgg gatgattgca tccaaaactt
    12961 caaaaaataa aaaactgtct attaattaga cagttatt
//
LOCUS       DAOQUR010000061        12841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5485_length_12841_cov_11.058893, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000061 DAOQUR010000000
VERSION     DAOQUR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5485_length_12841_cov_11.058893"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1981
                     /locus_tag="PKI50_10345"
     CDS             158..1981
                     /locus_tag="PKI50_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT04139.1"
                     /translation="MGKDTKQIAIIGGGYGGVHAARLLGKAARKGADISVLLADKKPY
                     HTLMTELHEVAGNRVEAESVQIDLREIFGASGVRVATEEIKSIDFKGRKLESESESYS
                     YDYLIIDTGAEPAFFGTPGVKENGFTLWSFEDAIRIREHVKNMFDKARTEKDHSRRRA
                     MLTFIVAGAGFTGIETMGELLEWRKELCREYEIDSREVSLMVVEAMGSILPILNERLI
                     KKSERYMKRHDVEIITSAPITEVKKESIILKDGREIPARTLIWTCGVQGSDFAAKTEL
                     THGGRGRIQVNEYMQSVDYQNVFAVGDNAFYEEPDGKGGKKPIPQIVEAALQTAETAV
                     HNILASIDKRGKKPLKSNYHGFMVSIGGRYAVANIGGRSMDGFFAMALKHLVNIHYQY
                     GVGGFARIWDYIMHEFIYVREGRSILGGHLSARTPILWLAILRVYLGVIWLIEGVKKI
                     NEGWLDPGKSFIAAMPRPEDVPLESTDAVTNATQVTQYYPEPLLKEPPRVFQWFMETF
                     IEPNAYLFQVLIVLTEAAIGLALIAGLFTILASLGSIFLCLNFILSAMAGSEIFWYIF
                     AGIAMFGGAGRAFGLDYYVIPWIKHRWKRTAFARRTHLYVR"
     gene            2068..4995
                     /locus_tag="PKI50_10350"
     CDS             2068..4995
                     /locus_tag="PKI50_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNT04140.1"
                     /translation="MQFKVGDIYHGFRLVEENDIKETNSKVRLFRHEKSGARLFYMEN
                     EDDNKVFSVTFRTPPRDSTGLPHILEHSVLCGSRKFPTKEPFVELVKGSLNTFLNAFT
                     FGDKTMYPVASRNDKDFENLMDVYLDSVFHPNIYKYPEILKQEGWHYELEDTSGELKY
                     KGVVYNEMKGALSSPESVLSRKLQETLFPDGPYGYESGGDPEVIPELTQEGFISFHKK
                     YYHPSNSYMFLYGKLDVLGRLKFIDEEYLKGFEAIHVDSAIPVQKAFEEMQSYELEYP
                     ISAQEDERDKTYLSLNYAVGLSTDPELYIAMEILEHILLATPASPLKKALIEADLGRD
                     VFGKYDNSILQPVFSIIVKNSNTEVLEDFKRVVSNTLEGLVRNGLDSQLAEAAVNLKE
                     FQLREADFEGYPKGLIYNIKIMDSWLYDKDPTMHLRYEHIFEKIRAEFNNRYFEGLIE
                     KYLLKNNHSSLLVLKPKKGLSEEKDKETKEKLRSIKAKMSEEQLRELVKETGELKKWQ
                     NTPDTPEQLALIPMLSIEDIDRKGEVLPQQVKEEAGVKVLAHHMFTNDIVYAGLLFDT
                     TAVPQELLPYIPLLSGILGRISTENYSYGELSKEIDMHTGGIRFSTQVYGEKDDDSKY
                     HPVFSIRGRALASKTEKMFELISEIVARSKFDDSRRIKEIIQENKSRIEMRISNEGYT
                     IACKRLFSYFSQEGSYLEAISGLTYYRFIADIERNFENRIEKVKENLQKLKRLIFRKE
                     GLIAGVTCEEDDYNRFAGGLGLVLKDLSNEKHPAFKYAFKVESLNEGLMTQGKVQYVA
                     QGYNFIKLGYPYTGKLQVLKTISRYSYLWNRIRVQGGAYGAFSGFEKNGNMFMVSYRD
                     PNLKETLKVYDEMYDYMRNFQIEEREMTKYIIGTISKLDLPLTPYMKGERAIENYMRK
                     ITSEDLQKEREEILGTRQQDIRELSDMMFELIKRRSYCVLGSEIKIRENSELFGSLVE
                     VFE"
     gene            5021..5935
                     /locus_tag="PKI50_10355"
     CDS             5021..5935
                     /locus_tag="PKI50_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNT04141.1"
                     /translation="MPLEIICNDITKMRVDAIVNAANSRLQQGGGACGAIFAAAGADK
                     LRDECNRIGYCPVGSAVITDGYALPAKHIIHTVGPVWQGGGYNEEKLLSSCYTNSLEL
                     ALKHGLGSVAFPLISSGIFGYPKDEALRVAVSAIGSFLLKYDMMVYIVIFDRAAYKIS
                     DKLFKPIKAYIDEKYAEKHTSRINPDDVGNRIGESFTEMLLRLIDESGKADPEVYKRA
                     NLDRKQFLKISSNLKYKPGKNTALALAVALELDLDRTADLLRRAGYALSPGSRFDLIV
                     EYFIREGNYNIFEINEALFAFDESLLGA"
     gene            complement(5982..7007)
                     /locus_tag="PKI50_10360"
     CDS             complement(5982..7007)
                     /locus_tag="PKI50_10360"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNT04142.1"
                     /translation="MKNAVNIEWNKLGFSYIKTDYRYISYWKEGSWDNGKLTSDNTLT
                     ISEGSPCLHYGQQCFEGLKAYTTKEGKIQLFRPDQNAKRMIKSCKRLFMPEVPEEKFI
                     DACIQVVKANEAWVPPYGTGATLYLRPLVIGIGDNIGVRPATEYIFTVFCMPVGPYFK
                     GGLTPLDFSVSDYDRAAPQGTGAAKVGGNYAASLLPHEQAREEGYADCIYLDPATHTK
                     IEEVGAANFFGITKDNRFVTPISDSILPSITKYSLLHVAKEYLELETEERDIYLDKLD
                     ELAEAGACGTAAVISPIGSITYKDKKHVFHSLTEVGPVTKKLYDTLVGIQFGDVKAPE
                     GWIVEVK"
     gene            7242..7967
                     /locus_tag="PKI50_10365"
     CDS             7242..7967
                     /locus_tag="PKI50_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002120909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04143.1"
                     /translation="MIELINVNKSYSGIVKAVDDLTLTIPEGQITGFLGPNGAGKTTT
                     IKIITGVLNADSGSITINGKNIRENPLEAKRQFGYVPDNPDMFLRLKGMEYLNFMADI
                     YNVPAELRRERIKGLAESFEMEAALTDQIQSYSHGMRQKIVIMGVLIHDPSVWILDEP
                     MTGLDPKSSFTLKEMMKTQAAAGKTVFFSTHVLEVAEKLCDRVAIINKGKLLFYGTME
                     EMRENFRSNGSLEKIFLELTENE"
     gene            7960..9633
                     /locus_tag="PKI50_10370"
     CDS             7960..9633
                     /locus_tag="PKI50_10370"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04144.1"
                     /translation="MNRYMVLTGVMLKNFNLLNLRPGKSGKNPIRGILLSLLIFAAFL
                     PMILGIGSLAMVGYEVLFEIRQEGLILVTGFSISSLTIFFFGIFYVMSVFYFSKDIES
                     LLPLPLKPSEILAAKFTVAMLYEYLTEAIFLGPILIGYGIASKAGLLYYLYGTLLFLA
                     LPVLPLVYASAISMLVMGFTNLAKNKDRFRIAGGITAMFLAVGINAFVTKLTSSTTSA
                     LELQKLLEQGNNSYAEIISGIFFSNRFAVLALLNSEVLKGFVNLALFMLICMLFVILF
                     LSFGEGVYYKGVVGISEASVKRKKFSETELERYGRQKPVLRAYTIKELKLLFRTPVYF
                     MNCVLTSLIFPLFIILPFIVQPDAMKELQGLSIYLSGERGSAIALAAAFGISVFVTST
                     NGIAASAISREGTNIFVCKYLPVSYKTQITAKVLSGMSIGMVGTLSMLLTASVFITVP
                     AYLMRLIVVVSIFGIAFSSFAGILIDLNFPKLIWDNEQKAVKQNFNVVLNMLVSVLVA
                     GIPIFTIIALHFTLWMTLLTLVLVFGILDLLLYNILSTIGVRLFERIEV"
     gene            9836..11917
                     /gene="fusA"
                     /locus_tag="PKI50_10375"
     CDS             9836..11917
                     /gene="fusA"
                     /locus_tag="PKI50_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT04145.1"
                     /translation="MKDYKTENLRNISLFGHGSAGKTTIAEAMLFNTGVLDRFGKVDD
                     GNTVTDFDPEEIKRKISISAATAPCDWKECRINAIDTPGYFDFVGEAVASLKVVDGAV
                     IALDSIGGVEVGAEKAWDFVNEGKKACVFFVNKLDRENASFSKVVEQLRGVFGNKIVP
                     FALPIGSEASFKGIVDIIDMKAKIREGNKTVEGDIPAELKAEAEEYRNAIIEAAAQTD
                     EELMDKYFGGEELSREEIIRGLRLGVVAGDVAPVLCGAAIKNIGIDVLMDAVMNYMPS
                     PADAQAPEAVNPKSNEKVKIPADVNAPFSAQVYKTVADPFVGKISMFKVMSGKLTADM
                     EVLNSSKEKKEKLSSLFLLRGKKQVAADKLVAGDIAAVAKLQFTTTGDTLCDANNPVV
                     FEPIQFPAPSISMAIEPKAKGDEDKIGNGLQRLVEEDPTLKVEKNTETKQTLISGMGE
                     QHLEVVTKKLQNKFGVEVVLKDPKIPFRETIRKSAKAEGKHKKQSGGHGQYGHVWVEF
                     EPILDGSADFEFVDKIVGGVVPRQYIPAVEKGLRECIEEGVLAGYPVVNLRCTLYDGS
                     FHPVDSAEMPFKIAASLAYKKGMAAASPVLLEPIYHVEVLVPDEYMGDIIGDLNKKRG
                     RILGMEPCGKMQKVSAEAPLAEMFKYATDLRSMTQARGSFTMEFERYEEVPAQIAQKI
                     IEAANAEREKE"
     gene            12222..12458
                     /locus_tag="PKI50_10380"
     CDS             12222..12458
                     /locus_tag="PKI50_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04146.1"
                     /translation="MVERMISVGGNVTLNDVRRIVDTVKHMDSNEQLVVSMNSQDSYN
                     AQNIFSVLEKNNFEWSTKGGHGGRDYYIIARKKL"
     gene            12480..12650
                     /locus_tag="PKI50_10385"
     CDS             12480..12650
                     /locus_tag="PKI50_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04147.1"
                     /translation="MDQNDNNKELEDKLNDISLYGEFMSTYNYWVSSEEQKNKAQHLQ
                     SIGKPKGTKNRK"
BASE COUNT         3836 a         2432 c         3279 g         3294 t
ORIGIN      
        1 atgctgattg ccggaaaaca gataaagcaa ggcacttcct tcgaagtgcc ttgcttgttt
       61 cattttattg ttttttgaag aattcatatt gcaaatgtat ttgtggaata cttcatatga
      121 ttgtatttaa aatcataagg aagtgaatct tgataaaatg ggaaaagata caaagcaaat
      181 agcaataata ggcggcggtt acggaggagt tcacgcagca aggcttttag gcaaggctgc
      241 ccgaaaagga gcggatataa gcgtgctgct ggcggacaaa aagccgtatc acaccttgat
      301 gacggaactc catgaggtgg caggaaacag agtagaggct gaatcggtac agatagacct
      361 tagggagata ttcggtgcat caggggttcg tgtggcaaca gaggaaatca agagcataga
      421 ttttaagggc cggaagcttg agtctgaatc agaaagctat tcctacgatt atctcattat
      481 agatacagga gcggaacccg cttttttcgg aacaccggga gtaaaggaaa acggttttac
      541 cctgtggtcc tttgaagacg caataaggat cagggaacat gtgaaaaata tgtttgacaa
      601 agccaggaca gaaaaggacc atagcagaag aagggcaatg cttacattta tcgtagcggg
      661 ggcaggcttt acggggatag agacaatggg ggagcttctg gagtggagaa aggagctttg
      721 cagggaatac gaaattgaca gcagggaagt gagccttatg gttgtggagg caatgggcag
      781 catactcccc atactcaatg agaggctcat aaagaaatcc gaaagatata tgaagaggca
      841 tgatgttgaa ataataacat cagcacccat aacagaggta aaaaaggagt ccataatact
      901 gaaggacgga agggagatac ctgccagaac cctcatctgg acctgcggcg tgcaggggag
      961 cgactttgcc gccaaaacag agcttactca cggaggcagg gggaggatac aggttaatga
     1021 atacatgcag tcggtagatt accaaaatgt ctttgccgta ggagacaatg cattctatga
     1081 agagccggac ggcaaaggcg gaaagaagcc aatcccccag attgtagaag cggcactcca
     1141 gactgcggaa acagcggtgc ataacatact tgcgtcaatt gacaagaggg gcaaaaagcc
     1201 cttaaagtcc aattaccacg ggttcatggt gtccatagga ggccggtatg cggtagccaa
     1261 cataggggga agatccatgg acggattctt cgcaatggcg ctgaagcacc tggtaaatat
     1321 ccactatcaa tatggggtgg gaggatttgc aaggatttgg gattatataa tgcatgaatt
     1381 tatttatgtg agggagggca ggtccatact gggaggacat ttgtccgcca ggacgcctat
     1441 actgtggctg gccatactca gggtatatct aggtgttata tggctcattg aaggtgtgaa
     1501 gaaaataaat gagggttggc ttgacccggg gaaaagcttt attgcggcaa tgccgaggcc
     1561 cgaggatgtc cccctggaat ctaccgatgc cgtaactaac gcaactcagg ttacacagta
     1621 ttatccggaa ccgctgctta aagagccccc ccgggtattt cagtggttca tggaaacctt
     1681 tatagagccg aatgcctacc tgttccaggt cctgattgta cttacagagg cagctatagg
     1741 ccttgcactt attgcaggat tattcaccat acttgcatct ctgggctcca tatttctgtg
     1801 cctgaatttc atattatcgg caatggcagg ctctgaaata ttctggtata tatttgccgg
     1861 cattgcaatg ttcggcggcg cagggagggc tttcggcctg gactattatg tgataccgtg
     1921 gatcaagcac cggtggaaaa gaaccgcttt tgcacggcgt acccatttat atgtaaggta
     1981 gtgttcacat aataatatgt aaaacacggc gggcatatat tataattaat ataaatgatg
     2041 aaatattttg ttggaggctg gaaagggatg caatttaaag ttggtgacat atatcacggt
     2101 ttccggttgg ttgaggaaaa tgatatcaag gaaacaaatt cgaaagttag actttttagg
     2161 catgaaaaga gcggagccag actcttttat atggaaaatg aagatgataa caaggtgttt
     2221 tcggttactt tcagaacacc ccccagggac agtacgggac tgccccatat actggaacat
     2281 tccgtgctct gcggctccag gaagtttccc acaaaggagc ccttcgtaga actggtgaaa
     2341 ggctccctga atacttttct gaatgctttt accttcggag acaagacgat gtatccggta
     2401 gcaagcagga acgacaagga ctttgagaac ctgatggatg tttatctgga ttcagtattc
     2461 catcccaaca tatacaaata tccggagatt cttaagcagg aaggctggca ctatgagctg
     2521 gaagacactt ccggggagct gaaatacaag ggtgtcgtat ataatgaaat gaagggggca
     2581 ctctcatcgc cggaatcggt gctttcgaga aagctccagg agaccttgtt cccggacggc
     2641 ccatacggtt acgaatccgg gggcgaccct gaggtgatac cggagcttac acaggagggg
     2701 tttatttcct tccataaaaa gtactatcat ccttcaaaca gttatatgtt cctgtatggc
     2761 aagctggatg tgcttggacg cctgaagttt attgatgaag aataccttaa gggttttgaa
     2821 gcaatccatg tggattcagc cataccggtg cagaaagctt ttgaggaaat gcaaagctat
     2881 gaactggaat accccatatc cgcccaggaa gatgaaagag acaagacata tctcagcctg
     2941 aattatgccg tagggctgtc taccgatccc gagctttata ttgcaatgga gatattggag
     3001 catatactgc tggcaactcc ggcatctcct ctgaagaaag cattgattga agccgatctt
     3061 ggcagggatg tttttggtaa atatgacaac agcatacttc agcctgtttt cagtattatt
     3121 gtaaaaaact caaatacaga agtcctggaa gattttaaaa gagtggtctc aaataccttg
     3181 gaaggccttg tccgtaacgg gctggacagt caattggcgg aggcggcggt caacctgaaa
     3241 gagttccaac tgagggaagc ggattttgaa ggttatccca agggtttgat atataatata
     3301 aaaatcatgg acagctggct ttacgacaag gaccccacaa tgcatctccg ttatgagcat
     3361 atatttgaaa agattagagc agaattcaat aacagatatt ttgaaggtct gatagaaaaa
     3421 tacttgctga aaaacaacca cagctcgcta ctggtgctta agcccaagaa aggcctgtca
     3481 gaggagaagg ataaagaaac aaaagaaaag ctcaggagca tcaaggcaaa aatgagcgaa
     3541 gagcagcttc gggagttggt gaaggagaca ggagagctta aaaagtggca gaatactcct
     3601 gatacacccg agcagcttgc gcttataccg atgctttcaa tagaggacat agacaggaag
     3661 ggcgaggtgc ttcctcagca ggttaaggag gaagcggggg ttaaggtgct tgcacaccac
     3721 atgtttacca atgacattgt atatgccggc cttttatttg atacaacggc ggtgccccag
     3781 gagctgctgc cgtatattcc tttgctgtca gggatactgg ggagaataag cactgaaaac
     3841 tacagctatg gggagctttc aaaggaaatt gacatgcata ccggaggcat caggttcagc
     3901 acacaggtct acggcgaaaa ggatgatgac agcaaatacc accctgtatt ttcaataagg
     3961 ggcagggctc ttgcaagcaa gacggaaaag atgtttgaac tcataagtga gattgttgca
     4021 aggtctaaat tcgatgattc cagaaggatc aaggaaataa tacaagagaa caagtccaga
     4081 atcgaaatga ggatatccaa tgagggatac acaattgcct gcaaaaggct gttctcgtac
     4141 ttctcccagg aagggagcta tctggaggct atttccgggc tgacttatta caggttcatc
     4201 gccgatatag aaaggaattt tgaaaacaga atagaaaagg tgaaagaaaa ccttcagaag
     4261 ctgaagagac tgatattcag aaaagaaggc cttattgcag gagtgacctg tgaagaggat
     4321 gattacaaca ggtttgccgg gggcctgggt ttggtgctta aggatttaag caatgagaag
     4381 catccggcgt ttaaatacgc ttttaaagtt gagtccctta acgaagggct gatgacccag
     4441 ggtaaggttc agtacgtagc tcagggttat aacttcatca aactgggtta tccctatacg
     4501 gggaaactgc aggtattaaa gacaatatcc agatacagct acctatggaa ccgtatcagg
     4561 gttcaggggg gagcttacgg cgctttttca ggctttgaaa agaacggcaa tatgtttatg
     4621 gtttcgtaca gggatccaaa cctgaaggaa accttaaagg tatacgatga aatgtatgac
     4681 tatatgagga attttcagat tgaagaaaga gaaatgacaa aatatattat cggaacaatc
     4741 agcaagctgg acttgccgtt aaccccgtat atgaaggggg aaagggccat tgagaattac
     4801 atgaggaaaa tcacatcaga ggatttgcag aaggagaggg aggaaattct cggcacaagg
     4861 cagcaggata tcagggaact gtccgatatg atgtttgaac ttataaagag acggagctat
     4921 tgcgtattgg ggagtgaaat aaaaatcagg gagaacagtg aactgttcgg aagtcttgtg
     4981 gaggtttttg agtaataata ttctgaaggg agtgtaatca atgccactgg aaattatctg
     5041 caacgatata actaaaatgc gtgttgacgc tattgtaaat gcagccaaca gccgcctgca
     5101 gcaagggggt ggtgcctgcg gcgcaatttt tgccgcagcc ggagcggaca agcttcggga
     5161 tgaatgtaac cgaatcggct attgcccggt tggaagcgcg gttattaccg acggttatgc
     5221 gctccctgcg aagcatatta ttcataccgt cggcccggta tggcaaggcg gcggttataa
     5281 cgaggaaaaa cttctttcct catgttatac aaattctctg gagcttgctt tgaagcatgg
     5341 gctcggttcc gttgccttcc ctctcatatc ttccggtatt ttcggatacc ccaaggacga
     5401 agctctgaga gtggctgtat cggccatcgg aagctttctc ctgaagtacg atatgatggt
     5461 ttatattgtc atatttgaca gggcggcata taaaataagt gataaattat ttaagccaat
     5521 taaggcatat atagatgaga agtatgcgga gaagcatact tcacgcataa atccggacga
     5581 tgtgggaaac cgtatcggcg agagctttac cgaaatgctg cttcggctta tcgatgaaag
     5641 cggtaaagcc gatccggagg tatataaacg cgccaacctt gaccgaaagc agtttttaaa
     5701 gataagcagc aatctgaagt ataagcccgg caagaacacc gcattagccc tggctgttgc
     5761 attggagctg gaccttgacc gaaccgccga tttgctccgc cgggcaggat atgccctatc
     5821 tcccggcagc cgctttgacc tgattgtgga gtatttcata agagaaggaa attataatat
     5881 ctttgagata aacgaggcat tattcgcttt tgatgaaagt ttgctggggg catgacagga
     5941 ttgcataaga ggcatatcat tatgggatat gcctctttct gttactttac ttccactatc
     6001 cagccttcgg gagcctttac atctccgaat tggattccga caagcgtatc atatagcttc
     6061 tttgttacag gccctacctc agtcaggctg tggaatacat gttttttgtc cttgtaggta
     6121 atgcttccta tgggcgaaat aactgcggcc gttccgcatg cccctgcttc agcaagctca
     6181 tcaagcttgt cgagatatat gtctctttct tccgtttcaa gctccaaata ttcttttgcc
     6241 acatgcagaa gggaatattt tgttatactg ggcaaaatgg aatcagatat gggagtgaca
     6301 aacctgttgt cttttgttat gccgaagaaa ttcgcagcgc ctacttcctc aatctttgta
     6361 tgggtcgccg gatcaagata tatgcagtcg gcataaccct cttcccttgc ctgttcatga
     6421 ggaagcagac ttgccgcata attgcctccc accttggccg cacctgttcc ctgaggcgct
     6481 gccctgtcat agtccgaaac cgagaaatcc aaaggtgtca atccgccttt aaaatatggg
     6541 cctaccggca tgcagaatac tgtaaatata tattcagtgg cgggtctcac accgatattg
     6601 tctccgatac caatgaccag aggcctgagg taaagtgtag cccccgttcc gtaaggagga
     6661 acccaggctt cattggcttt gactacctgt atacaagcat caatgaattt ttcttcgggt
     6721 acttccggca tgaagagtcg tttacagctc tttatcatac gctttgcatt ctggtcgggc
     6781 ctgaaaagct gtattttgcc ttcctttgtg gtgtaagcct tcaaaccttc aaagcactgc
     6841 tggccgtaat gcaggcaggg ggaaccctcg ctgattgtga gagtgttgtc ggaagtaagc
     6901 ttcccattgt cccaggagcc ttccttccag tatgaaatat agcggtagtc cgtttttatg
     6961 taggaaaacc ccagcttatt ccattcgata ttaacagcat ttttcattaa ttcattcctc
     7021 caagtaaata atgttaataa atgattatat caatatcata agataatagc ttccctttgt
     7081 aaatagtatg ctgtagaata ctgtaagtcc agggtatgta tggaaggaac actaaagtgt
     7141 atgaaagcct tccgatagtg aaatattatt tagaaggctt tatttttctg acatatatgt
     7201 tataattaaa tggcaaaaat agccagccgg gaggagaata catgatagag ctgattaatg
     7261 taaacaagtc atacagcggt attgtaaagg cagtggatga tctgactctc accatcccgg
     7321 aaggtcagat cacaggattt ctgggcccca atggggcggg aaagacaact accataaaaa
     7381 ttataacggg agtactgaat gccgacagcg gaagcataac cattaatggg aaaaatatca
     7441 gggagaatcc tcttgaggca aaaaggcagt tcggttatgt ccctgataac cccgatatgt
     7501 tccttcgctt gaagggtatg gagtacctga atttcatggc tgacatttat aatgtccccg
     7561 ctgaactgag aagggaaagg attaaggggc ttgctgaaag ctttgaaatg gaagcggccc
     7621 ttacggatca gatacagagc tattctcatg gaatgagaca gaagatagtc attatgggag
     7681 tgcttatcca tgatccttcg gtgtggatat tggatgagcc gatgacggga ctcgacccca
     7741 agtcctcctt cactctcaag gagatgatga aaacccaagc agccgccggt aaaacggtgt
     7801 tcttctccac ccacgtcctt gaggtggcag agaagctgtg cgacagggtg gccattatca
     7861 ataaaggcaa gctgctgttc tacggtacta tggaggagat gcgggaaaac ttcagaagca
     7921 acgggtctct tgaaaaaata ttcctggagc tgacggaaaa tgaatagata tatggtactt
     7981 accggagtaa tgctgaaaaa tttcaatttg ctgaatttga ggccggggaa atccggcaaa
     8041 aatcccataa ggggaatatt gctatccctg ctgatatttg ccgcgttttt acccatgata
     8101 ttaggtatcg gcagtctggc catggtagga tatgaagtgc tttttgagat ccggcaggag
     8161 ggtctgatcc ttgtaacagg cttttccata tccagcctga caatattctt tttcggcata
     8221 ttctacgtta tgagtgtttt ttatttttcc aaggacatcg aaagcctgct gccgctgcct
     8281 ttaaagccgt cggaaatact ggcggccaag tttacagttg ctatgcttta tgaatacctg
     8341 acagaggcga tattcctggg gcctattctc ataggctacg gaattgcaag caaggcgggg
     8401 ttgttatact atttatacgg tacccttctg tttcttgcgt tgccggtgct gccccttgta
     8461 tatgcctctg caataagcat gctggtaatg gggtttacca accttgccaa aaataaagac
     8521 aggttcagga tagcgggcgg gataacagcc atgtttttgg ctgtagggat aaacgctttt
     8581 gtaacaaaac tcaccagttc caccacgagc gccctggagc ttcagaaact gctggaacag
     8641 ggcaacaatt cctatgcgga aatcatatcg ggtatttttt tcagcaatag gtttgcggtg
     8701 ctggcgctgc tgaacagtga agtactgaag ggctttgtga acctggctct gtttatgctt
     8761 atttgtatgc tttttgtgat tttgttcctg agctttggag aaggagtgta ttacaaaggt
     8821 gtggtgggca tatcggaggc ttccgtaaaa aggaagaagt tttcagaaac cgagcttgaa
     8881 aggtacggca ggcagaagcc ggttctgagg gcatatacca taaaggagct taagctgctt
     8941 ttcaggactc ctgtatattt tatgaactgt gtgctgacga gtttaatttt tccgttattt
     9001 attatactgc cttttattgt gcagcctgat gcaatgaagg agcttcaagg attaagcata
     9061 tatttaagtg gtgaaagagg gagtgccata gctttggcgg cggccttcgg cataagtgtt
     9121 tttgtcactt caacaaatgg aattgcagct tccgcaatat ccagggaggg cactaacata
     9181 tttgtgtgca agtatctgcc tgtaagctat aagactcaga taacggcaaa ggtgctatcc
     9241 ggtatgagca taggtatggt tggcacactt tcaatgctgt tgacagcttc agtatttata
     9301 acggttccgg cctacctcat gcggcttatt gttgttgtaa gtatttttgg aatagctttc
     9361 agctcctttg cgggaatact tatagacctg aactttccaa agcttatatg ggataatgaa
     9421 cagaaggcgg taaagcaaaa ttttaatgtt gtgctcaata tgcttgtcag tgtattagtc
     9481 gcgggtatac cgatttttac aattatagca ttgcacttta ccctgtggat gacattgctt
     9541 acactggtgc tggtctttgg gatattggac ctgctgctgt acaatatact ctcaacgata
     9601 ggggtaaggc tttttgaaag gatagaggtt tgaaaccagt catttgttat tttcctcttt
     9661 acatttattt cacggaaaga taaaattaga gttacagtgc acatatatcc tcagggcaca
     9721 cattggaaag tgtgctgata cattgaacag caggaagcag ggtatgttaa taaagtattt
     9781 gcacgtttaa aattgctaat gacggacaga ataaacataa aaagggggaa tttttatgaa
     9841 agattacaaa acggaaaacc taaggaatat tagtttattt ggccacggca gcgccggaaa
     9901 gacaacaata gcagaagcta tgctgtttaa taccggagtt ctggataggt tcggaaaggt
     9961 tgatgatgga aatactgtta cagactttga cccggaagaa atcaaaagaa aaatttccat
    10021 tagtgcggca acagctccat gtgactggaa ggaatgcaga atcaatgcaa ttgatactcc
    10081 cggatatttc gactttgtag gtgaagcagt cgcaagcctg aaggttgttg acggagcggt
    10141 aatagctctt gacagcatcg gaggagttga agtaggtgcg gagaaggctt gggattttgt
    10201 taacgaaggc aagaaggcat gtgtgttctt cgtaaacaag cttgacagag aaaatgcaag
    10261 cttctcgaag gttgttgaac agcttagggg agtttttgga aataaaatag ttccttttgc
    10321 tcttccgata ggatcagaag caagcttcaa gggaatagtc gatattatag atatgaaggc
    10381 caagatcaga gagggcaata aaactgttga aggggatatt cccgcagaac tcaaggctga
    10441 agccgaagaa tacaggaatg cgataataga agcagcggcc cagactgatg aagaacttat
    10501 ggacaaatac ttcggcggcg aagagctttc aagagaagag ataataagag ggctgaggct
    10561 tggagttgta gccggggatg tagcgccggt actatgcggc gctgcaataa agaatatagg
    10621 aatagatgtg ctcatggacg ctgtaatgaa ttatatgccg tcaccggctg atgcacaggc
    10681 tcctgaagca gtcaacccaa agagcaatga gaaggttaag attcccgctg atgtaaatgc
    10741 tcctttcagc gcacaggtat acaaaacagt tgccgacccc tttgtcggaa agatttcaat
    10801 gttcaaggta atgtcgggaa aattgactgc agatatggaa gtattgaaca gcagcaagga
    10861 aaagaaggaa aagctcagca gcctgttcct tttaagaggt aagaagcagg tggctgcaga
    10921 taagcttgtg gcaggagata tagctgcagt tgcaaaatta cagtttacta ctacaggaga
    10981 taccctgtgt gatgccaata atccggtagt atttgaaccc atacaattcc cggctccaag
    11041 tatttcaatg gctattgagc ccaaggcaaa aggggacgaa gataagatag gaaacggcct
    11101 gcaaaggctt gttgaagaag atcctacgtt aaaagtggag aagaacaccg aaacaaaaca
    11161 gactttgatt tcgggaatgg gcgagcagca tcttgaagtt gtaacaaaga agctgcagaa
    11221 taagtttggc gtggaagtgg ttcttaagga tcccaagata cctttcagag aaaccataag
    11281 aaaatcggct aaggctgaag ggaagcataa gaagcagtcc ggcggacatg gacagtacgg
    11341 ccatgtttgg gttgagtttg agccgatact tgacggaagc gctgattttg aatttgttga
    11401 caagatagtt ggaggcgtag ttccaagaca gtatatacct gcagttgaga agggactcag
    11461 ggaatgcatc gaagaaggtg tgctggcagg ttatccggtt gttaatctca gatgcactct
    11521 ttatgacgga tccttccacc ctgttgactc ggcggaaatg ccttttaaga ttgcggcatc
    11581 tctggcatat aagaagggta tggcagctgc aagccccgta ttgcttgagc caatatatca
    11641 tgtggaagtg ctggttccgg atgaatacat gggagatata atcggagatt tgaacaaaaa
    11701 gagaggcagg attttaggaa tggaaccctg cggaaagatg cagaaggttt ctgcggaagc
    11761 acctttggct gagatgttca aatatgctac cgacttaaga tctatgaccc aggcaagagg
    11821 aagctttacg atggaatttg aaagatatga ggaagtacct gcacagatag cccagaagat
    11881 tatcgaagcg gctaatgcag aaagagaaaa ggaataggga acagaaacaa catggcaaat
    11941 taaaaacttg ccatgttttt tattgctttt ttttatttta taggaaagga tactttccta
    12001 taaaatgggg ggatgcaagg gggagaatcc acctgccgtg gaaggagggt gctcaggatg
    12061 cattctgcat ccgtcgaagg gaaccatagg gttccctagc gaaaaaaaac gtcgtgagac
    12121 agtttttttt gttgtgtata tttaaaaaac agtttcataa actataaatg agcaatttca
    12181 tggatttttg tgaaaagcac tcaataatag gaggcagttg aatggttgag aggatgataa
    12241 gtgttggagg aaatgttacg ctgaacgatg tgagaagaat tgttgatact gtcaagcata
    12301 tggattcaaa tgaacagttg gtggtatcaa tgaacagtca ggattcatac aatgctcaaa
    12361 atattttttc agtactggaa aaaaacaact ttgagtggtc taccaaaggc ggacatgggg
    12421 gcagggacta ctacatcata gccagaaaaa agttataaac taccataagg aggagtaaca
    12481 tggatcaaaa tgataataac aaggaacttg aagacaaact taacgatatc agcttgtacg
    12541 gagaattcat gtctacctac aattattggg tgagttcaga agaacagaaa aacaaggctc
    12601 agcacttgca gtccatagga aagcccaagg gaacaaaaaa cagaaaataa attgtatcaa
    12661 aaaaggggtt tcgcaatact atcgaatata tagtttgcga aatccctttt tgcatcagaa
    12721 gaataatgcg acggggacat ggggacacgg ggacgtttcc cctgtcccac ttccagtggg
    12781 acacgcgaaa cgtccccgtg tccctgcgtc cccgtcgcat tattctttta tgctgcataa
    12841 a
//
LOCUS       DAOQUR010000062        12735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5561_length_12735_cov_10.724054, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000062 DAOQUR010000000
VERSION     DAOQUR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5561_length_12735_cov_10.724054"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..862
                     /locus_tag="PKI50_10390"
     CDS             140..862
                     /locus_tag="PKI50_10390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT04148.1"
                     /translation="MTDTILFDLDGTLLPLDMEKFTKIYFHEMGNMFNDMIDPKLLAK
                     YIWAATLDMVGNTEYKTNEEVFMDSFSKLIDGDINKYKERFDYFYDTLFHKTKEAAEI
                     QSLIIESISILKKKNYKLVIATNPLFPRKAIHRRIEWAGLDLKDFEYITSYEDNHFCK
                     PQPEFYKEVLDIIGKRPEQCLMVGNDVQEDLIALKLGIKTFLIKDNMIHRTEEAFTST
                     YEGNYEDFYSFVKELPDVSRSS"
     gene            complement(846..1640)
                     /locus_tag="PKI50_10395"
     CDS             complement(846..1640)
                     /locus_tag="PKI50_10395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNT04149.1"
                     /translation="MRKIPEKIYKELEKNIYDGISLPKSAIVLFWTVLCLYFVNMKTS
                     RLYAVFFGILFYASLLVLCLILFKDIKRYLSFPKDNNILPRFIGYKPDIILIYPIFLM
                     FLVGAINSLLIASGIFPEALQKMSAVTSGPIELFAKIIALPLAAFTEELINLLLVSLA
                     FNKMKFPGNIRLIISILLAALTFGMLHAIGWGLKIALLIGISYIPVFLATLYTGNIWI
                     SFLAHFYNNFISLFKAYYGNYHFFIVAAICLIPFIWAINAIVRKSG"
     gene            complement(1768..1843)
                     /locus_tag="PKI50_10400"
     tRNA            complement(1768..1843)
                     /locus_tag="PKI50_10400"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1808..1810),aa:Lys,seq:ctt)
     gene            complement(1948..3813)
                     /locus_tag="PKI50_10405"
     CDS             complement(1948..3813)
                     /locus_tag="PKI50_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNT04150.1"
                     /translation="MRESGRAIEKYQPLWGSWKVDELIGQGVDCEIYRVFKEEWGKRY
                     ISTVKFMSFSISKNDISEAQAIGISRAAMPEYFKSLLVNVQNEIELMYKLRGNSNIVT
                     YEDHGIYEKKGNQGWDVLIRMESLQALPDFLIGRKLGRLDVVRLGIDICKALEACGRE
                     NIIHRDIKDSNIFVSPREEFKLGSFNMAKELSKGGRTVLTALNPLYMAPELYKEQSYD
                     FSVDLYSLGIVMYKLLNKGRLPFLPLPPEAITVEDTEKSAARRMSGERLPLPADSEEN
                     LGAVILKACSYDWKDRYKSPAEFRQKLERILKAEAKTTMNNQEIQAEDKESIEISKKN
                     EEEAERTAVAEIAASINNASGGSKRRFFSAAAIIVAAMVLAFMIGYIMNYDALPATLE
                     PACEEIAETAEVIPAPAATAVLTTVPAAEPVKTESRIKTAKKRTEAEKLKGKALEYYE
                     KNEYGKAIEVFSELIKVDASYRGFRQYSDSFLQLAREHNLAGVKQYNEGRLEQAVKEF
                     DNALNMLETMKSNTSNYDMELYSKLKELFAGNKDRMLEKAEKIDACFKLANECNLAGV
                     KYYNEGSFKKAKLEFENAIKHLSEIRLLVSKYSGNSYESLMEIYKGNLSRTEERL"
     gene            4062..4433
                     /locus_tag="PKI50_10410"
     CDS             4062..4433
                     /locus_tag="PKI50_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HNT04151.1"
                     /translation="MSLLSQTIQSGDWKGEKHVPVIHCPEKVTPDTPFEVKVTIGDAI
                     PHPNTFEHHIVWIKVFFQPEGSKFPAEIAHFNFSAHGEGETFTDPAGLTRIKIKKSGI
                     IYALSYCNIHGLWESSKEISL"
     gene            complement(4503..4682)
                     /locus_tag="PKI50_10415"
     CDS             complement(4503..4682)
                     /locus_tag="PKI50_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04152.1"
                     /translation="MAYSLVQDEPKPDDIIVRAEDRTFYVQNDISDNVDFFEIDFHNG
                     WFRKVFVVFPNGESY"
     gene            complement(4939..5391)
                     /locus_tag="PKI50_10420"
     CDS             complement(4939..5391)
                     /locus_tag="PKI50_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT04153.1"
                     /translation="MFMVPYGRKNRGLTERPHDIFNIDNLFDGFFGDSFMPAFFAGDA
                     QIKVDIKENEKEYIVEADLPGVKKEEINVDLNNDRLTISVTRNEETNIEKENYIRKER
                     RSGSYSRSFYVENVIENQISAKFENGVLSMVLPKRESGVSKNKRIEIQ"
     gene            5722..6921
                     /locus_tag="PKI50_10425"
     CDS             5722..6921
                     /locus_tag="PKI50_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT04154.1"
                     /translation="MIEYIKNWLALKGMNEGISLILSNIITAIAIVLLSIVINLIVKK
                     FFLKIVKAYAAKSPQKWDDILLKNKFFDQIVNIVPGFIIYGFAPVIPMYQNLIQRIAS
                     AYIVIAVLFVVHKLLNAVDDIYRSSQEERTRPIKGFLQVIEIISIIIGIILIISFLIN
                     RSPWIMLSGIGAATAIFSLIFKDSILGFVAGIQLTANNMVKLGDWIEMPKYDADGDVI
                     EITLHTVKVQNWNKTISMIPTYALISDSFKNWTGMQEFGGRRIKRHINIDLTSIKFCT
                     EEMLERLVKTPYLQEFLSSYNNEHINGKQLTNIDAFRAYLVGYLKNHPGLNKEMTQMV
                     RQRQPTEFGLPIEIYVFFDGTKWADYEVVQSEIFSHVIAMVQEFDLRIYQNPSGYDFR
                     NLGKCES"
     gene            complement(6983..8980)
                     /locus_tag="PKI50_10430"
     CDS             complement(6983..8980)
                     /locus_tag="PKI50_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04155.1"
                     /translation="MINNKSREYIVKKTLLFERAFSMDNYNGDSKIDYGYKYEKGTIP
                     VLVSAPHSVNHLRNGKVKCADIFTGSIARMIHLLTGCHVFYKTKNDNYDPNYDPINGD
                     GDYKNSVIDVIRKENIRIFIDLHGAAENREFDIDLGTDGSNNLCGNNFIPDLFRVVFG
                     KYGIHKVEENKMFTASPQNNVSRTMAAVCGIPSIQVEVNRKYREVSVNEEEFMSFMKG
                     LYSVISVLNDCEWDSDTYVFEAEKSKIHIPIDKIEFSREDAEKYGFQRNDSFQVNSVF
                     NHCSKTEFIARYKHTGNNQEFVPGKVYLTNKLYKNVFGDNEAEERKYVLVNRKKAITL
                     PIGIPKAGSERIMICPDLADKIDLSKNYQLYNRHDDIDYCQGKLSVTRDTTAKGKIFL
                     NYYQRHIMNVNIPKKVILRNDFLKYLGSDVLTEAAKDILKRSYKDRHSYYEIEQNLIE
                     DDDLKSIFKKLELDKIELVEVNDTGDKTKAMGLGGRWRRFIYGILSFLIKRKSICLRV
                     GRPYPTDENSDIVRIMPTTMKILGIGETDKLIVRHRGHEAILRALPFDSFEVLKNSNV
                     LVYDDADASTLIGIPSKYRVELGIYSLNSIVSAERDMKYLFFKNSNVQLLPIIAVIFT
                     IIQTFQDTATRILLSCILVPLAIYVSLSQERLKVDSDSKRK"
     gene            complement(9006..10367)
                     /locus_tag="PKI50_10435"
     CDS             complement(9006..10367)
                     /locus_tag="PKI50_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNT04156.1"
                     /translation="MIGVVAIFTNAKEAEDFVYSSYMNAIDKIPKDMPDMVTRNPHLT
                     RALLDKTEEPDKKQRNILVTGSKGKGSVSRMISKLIEAHGLKAGLCTSPHLVSFLERI
                     RINGNAISEGDFVKYANILEPYAAEVQKQLTEAQYIGPAGITAVIAMLYYLDQKTDYN
                     VFECGKGARYDDVTRIYSEASVINTIFPEHIPQLGYNLPEIAFNKAGIINSIQRFAVS
                     AVQQKEVRDVICREACDKGVRLFTFGQDFLCEKICVDRNGTYFDIITRNNKYRNIRLN
                     LMGRHQAYNAAIAVCTAENLLGGVKEENIRQAFDGITWPGRLEVINRSPLTILDGCIN
                     RECAIHVREFVSEMGNKDVISIVGIPDDKDYEGVACEMYDISRRIIFTASKNKHLKFT
                     TAQMDKAKPMLGEKLIYEAEIEDAIETAYNLIGDDGLVCIIGTQSLVRDVKEYFKQDT
                     LNL"
     gene            10641..11153
                     /locus_tag="PKI50_10440"
     CDS             10641..11153
                     /locus_tag="PKI50_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04157.1"
                     /translation="MFNKRILFFCTVLALLAAALQAVPLIFGAAFAFGALLNGFPIYL
                     AARLSYIPGILVYLSAAALLAFLNPFEALFFICTNGIIGLSLGIIKDRFRSIYPVPAL
                     SALLDVAILFTVNYRFGISIFSPSAFSTPAGQAAALLFPMYLYCLIYLKLAMSLDKLI
                     HRYIELDIHP"
     gene            complement(11157..12107)
                     /locus_tag="PKI50_10445"
     CDS             complement(11157..12107)
                     /locus_tag="PKI50_10445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT04158.1"
                     /translation="MGRYFIKALIFCILFFSVCGVIGLQEDFFVPEKAEPVTEPEPEP
                     VPEPVPEIVFSETELLPGDCFAIYLKGLTKRDDIQVSTAFVQGQPRFFSFMEGRLAII
                     GTSCRTKEGDYSCKVQVLRDGEIAAEKEETFKVFHKEFVEQHLTVTSTQKEQRSDDNF
                     DMDKVHTERAKSVTSEKPLWEGTFVKPVEGRVSTEFGMIRYINKEESGRHSGIDIAAA
                     RGTPVKAANNGVVRLSMMLNVTGNTIIIDHGCNIYSSYAHLDKLLVEEGAEVKKGDII
                     GEVGSTGFSTGPHLHWSTTIGTLYINPESLTESDPLAFIR"
     gene            complement(12187..12621)
                     /locus_tag="PKI50_10450"
     CDS             complement(12187..12621)
                     /locus_tag="PKI50_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT04159.1"
                     /translation="MDMMQLVLFRLNEQEYAIDIFKVNEIVPYRKPTMIPNTPDYVEG
                     VLNLRGNVIPVISLKKRFQLEDKSTEENTKIIVADTGGTIAGFVVDEASNVITVEREK
                     IEPVSEVVSNIGSKYINGVGKKDDGSMYILLNFDGLYAEGVL"
BASE COUNT         3489 a         2854 c         2249 g         4143 t
ORIGIN      
        1 atgcgacatt tataggaaac tataaatgtc gcatattgtt tgcccgtatt ccacatcatg
       61 tgcatttaca tttccctgat tattatatat attatacctt gaaagattgt ttcgcagatt
      121 atataaaagg agaatacaga tgacagatac tattcttttt gatttggacg gaaccttatt
      181 gcccctggat atggagaagt tcactaaaat atacttccat gagatgggaa acatgttcaa
      241 tgatatgata gacccgaagc tgcttgccaa atatatatgg gctgctactt tagatatggt
      301 cggcaatact gaatacaaaa caaacgaaga ggtattcatg gatagctttt caaagcttat
      361 tgacggtgat atcaataagt acaaggagcg gttcgattat ttctatgata ccctgttcca
      421 taaaacaaaa gaggctgccg aaatacaatc attaattatt gagagtattt caatcctcaa
      481 gaaaaaaaac tacaagcttg taattgcaac caatccgttg tttccccgca aagcgataca
      541 ccgcaggatt gaatgggcag ggcttgattt gaaagatttt gaatacataa cttcatatga
      601 agacaatcat ttctgcaaac cccagcctga attctacaag gaggtgctgg atatcatagg
      661 caagaggccg gagcagtgct tgatggtcgg aaatgatgtt caggaggatc ttatagctct
      721 taagctcggg atcaaaacct ttttgataaa ggataatatg attcatagga cagaagaagc
      781 atttaccagc acttatgaag gcaattacga ggatttttac agctttgtaa aagaacttcc
      841 cgatgtcagc cgctcttcct gactatggca tttattgccc aaataaaagg tatgagacaa
      901 attgcagcca ctataaaaaa atgataattg ccgtaataag ctttaaataa ggaaataaaa
      961 ttattataga aatgtgccag aaaacttatc cagatgttac ctgtatataa ggtggctaag
     1021 aacacaggta tgtaagatat tccaataaga agtgcaattt ttagccccca gccgatagca
     1081 tgcagcatcc cgaaagtcaa agccgcaaga agtatgctga taataagcct gatattgccg
     1141 ggaaacttca ttttattaaa agccaaagaa accagaagaa gattaatcag ttcttctgta
     1201 aaagctgcta aaggtaaggc tataatcttg gcaaaaagct caatggggcc gcttgtcact
     1261 gcactcatct tctgaagggc ttcggggaaa attcctgatg caataagcaa actgtttatt
     1321 gcaccaacaa gaaacatcaa aaaaatcgga tagataagta ttatgtccgg tttgtatccg
     1381 ataaaacggg gaagtatatt attatcctta ggaaagctca ggtatctttt tatatccttg
     1441 aaaagtataa ggcacaagac cagcagagaa gcgtagaata aaattccgaa aaaaacagca
     1501 tataaacggc ttgtcttcat attaacaaaa taaagacaca aaaccgtcca aaagagcact
     1561 attgcgctct ttggcaggct tatgccatca taaatattct tttccaattc tttataaatt
     1621 ttttccggta tttttctcat gaacacatgc ctcatattca taataatact tgacctgatt
     1681 ggctttcact tatcatatta tatcataaat ccgcaagtat ctaataaaaa aaacactgca
     1741 aaccgttgca gtgtatacat tctttgatgg tgcgccatca gggactcgaa cccgggaccc
     1801 attgattaag agtcaattgc tctaccaact gagctaatgg cgcacgtttc gtctattctg
     1861 ttctcaatcg actttataat tatacaaaag aaaattatct gtgtcaacat gttattattg
     1921 gattaaattt taatttttca ggatgattta tagtctttct tccgtgcggc tcaaatttcc
     1981 cttatatatt tccattaagc tctcgtagct gttgccggag tatttggaaa ccaacagcct
     2041 tatttctgaa agatgcttta ttgcattttc gaattcaagc tttgcctttt taaaggaacc
     2101 ctcattataa tatttcactc cggctaaatt gcattcattg gccagcttga agcatgcatc
     2161 tattttttca gccttttcaa gcattctgtc cttatttccc gcaaaaagct ctttaagttt
     2221 ggaataaagc tccatatcat agttgcttgt atttgatttc atagtttcaa gcatgttcag
     2281 cgcattgtca aattccttta ctgcctgttc aagcctgcct tcgttatatt gcttcactcc
     2341 ggccagattg tgctccctgg caagctgcag aaaagaatca gagtattgcc tgaaacctct
     2401 gtaagaggca tcaactttta ttagttccga aaacacttca attgcttttc cgtattcatt
     2461 tttttcatag tattccaatg cttttccctt cagcttttca gcttcggttc ttttttttgc
     2521 tgtcttgatc cggctttccg ttttaactgg ttctgcagca ggtacagtag tcaataccgc
     2581 tgttgccgcc ggtgccggaa ttacctcggc tgtctcggca atttcctcgc aagccggctc
     2641 taatgttgcc ggtaaggcat cataattcat tatgtatcct atcataaatg ccagtaccat
     2701 tgcagcgaca attatggctg cagcagaaaa aaaccttctc tttgaccctc ctgatgcatt
     2761 attgatggat gcggcaatct ccgccactgc tgttctttct gcttcctcct cgtttttttt
     2821 tgaaatctca atactctctt tatcctccgc ttgaatttct tgattgttca tcgttgtttt
     2881 agcctccgcc ttaagtatgc gttcaagctt ttgcctgaat tccgccggcg acttatatct
     2941 gtccttccag tcatagctgc aggctttaag gataacagcc ccaagatttt cttccgaatc
     3001 tgcaggcagc ggcagccttt cccctgacat cctgcgggct gcagactttt ccgtatcttc
     3061 aacggtaata gcttccggag gcagcggcaa aaatggcagc cgccccttgt taagcagctt
     3121 gtacataact attcccaaag aatacaggtc aacggaaaaa tcatacgact gctctttata
     3181 aagttcgggg gccatatata agggattgag tgcagtcaaa acagtcctcc cgccctttga
     3241 aagctctttt gccatattga agctgcccaa cttgaactct tccctgggtg atacaaatat
     3301 atttgaatcc ttaatgtccc tgtgtattat gttctccctt ccgcaggctt caagagcctt
     3361 gcagatatca atccccagcc tgacaacatc aagccttccg agttttcttc caattagaaa
     3421 atcgggcaat gcctgaagag attccatcct tataagaaca tcccacccct gatttccttt
     3481 cttctcatat atcccatggt cttcgtaagt tacaatattg ctgttccctc tcagcttgta
     3541 catcagctca atttcgttct gtacgttgac caacaggctt ttaaagtatt caggcatagc
     3601 tgccctgctg atgcctatgg cctgagcctc ggaaatatca tttttgctta tggaaaagct
     3661 catgaatttg acggttgaaa tataccgttt accccattcc tccttaaaca ctctgtatat
     3721 ttcacagtca accccttgtc caataagctc atctaccttc catgaccccc aaagaggctg
     3781 gtatttctca atggccctgc cggattccct catccaattc accccaaatt catctgaaga
     3841 ctcacttaat tatgaaacct gcacctttaa ctcataacct ataatctagt aatttctata
     3901 ttcggcagcg gtatccttca ttatttcaat cgcaagttcg gacaccattt ttctacatat
     3961 tcatacaaag gttctatgga aaattcatct ggatttatcc atgctatata atatataata
     4021 taatatataa tataaagtaa tatatttggg agggtgataa tatgagtctg ttaagccaaa
     4081 caattcaatc aggtgactgg aaaggtgaaa agcatgttcc tgtaattcac tgccctgaaa
     4141 aggtaacgcc ggataccccc tttgaagtaa aggtgacaat cggtgatgca ataccccatc
     4201 ccaatacctt tgagcaccat atagtatgga taaaggtgtt tttccagccg gaaggctcaa
     4261 aatttcctgc agaaatagct catttcaact tctcggctca tggagaaggg gagaccttta
     4321 ccgacccggc agggctgacc cggataaaaa taaaaaaatc cggaatcatt tatgcattgt
     4381 cttactgcaa tatccacggg ttgtgggaaa gcagcaaaga aatcagtctt taggttaaaa
     4441 gaaagccctg atgtattata catcagggca gtttcatgaa ttcgccttgc tctttaaaat
     4501 gcctaatagc tttctccgtt gggaaaaaca acaaatacct tgcggaacca tccgttatgg
     4561 aagtctattt caaaaaaatc tacattgtca gatatgtcat tctgcacata gaaggttcta
     4621 tcttctgcac ggactatgat gtcatccggt tttggttcat cctgaaccag gctgtaagcc
     4681 atagacagtc atccgcaatc attatcatat tctattctga agttcttgga ttcaattttt
     4741 gacagttccc ctttagcttt atcagtaatc ctgacatcca taatatccct ccattcgtgt
     4801 ttgaatgtta gttcaattat gaattatttg ataaataaaa tcaagattaa tttgtaatag
     4861 ttattaattt gtcaatatac aggatttcct gcaaaaagcg gttataccgc agctttcggc
     4921 tgcggtacaa ccctggcatt attgaatctc aattctctta tttttgctta ctcctgattc
     4981 cctttttgga agtaccattg ataatacgcc attttcaaac ttggcagaaa tttggttttc
     5041 aattacattt tcaacataga agcttctgct gtagcttccg cttcttcttt cttttctgat
     5101 atagttttct ttttctatgt ttgtttcttc gtttcttgtt actgatattg tgagtctgtc
     5161 attattaagg tctacgttga tttcttcctt ttttactccg ggtaaatctg cttcaacaat
     5221 atactctttt tcattttcct ttatatccac cttaatttgt gcatcacctg cgaagaatgc
     5281 gggcatgaag gaatctccga agaaaccgtc aaatagattg tctatgttga aaatatcatg
     5341 aggcctctct gttaagcctc tattttttct gccgtatggt accatgaaca tacaaaacac
     5401 ctccgtataa atgtggtaat tttggtctta atttgacttt gactttcttt gacctttgtt
     5461 tatattatca catttattat gggaaatttc aattttcatt attccttatt ttttgttaaa
     5521 ttagttcaaa tttacataat atataaaaat atcctcgttt ttctcaaaaa atctttgttt
     5581 taaatattta cccttcttta tttttcaata attcatattt tattgtcgca gctgtacttg
     5641 cataattatc aattatttta aataatatga tataattatt gcaataccga agtacaaata
     5701 ctgcatacgg aggtgctgca aatgattgaa tatataaaga attggctggc tcttaaaggt
     5761 atgaatgaag gaatttctct tattttatca aatattatca cagctatcgc tattgtgctt
     5821 ctcagcatag tgataaattt gattgtaaaa aaattctttt tgaaaatcgt taaggcatat
     5881 gctgcaaaat cccctcaaaa gtgggatgac attttgctga agaacaaatt ctttgatcag
     5941 atagtgaata ttgtacccgg atttataatc tacgggtttg caccggttat ccccatgtat
     6001 cagaacttga tccagcggat tgcctccgcc tatatagtta ttgctgtttt attcgttgta
     6061 cataagcttc tgaatgccgt ggatgatatt tacagaagca gtcaggaaga aagaacaagg
     6121 cccataaaag ggttccttca ggttattgag atcatttcaa ttattatagg aatcatatta
     6181 atcatcagct ttcttataaa tcgttccccc tggattatgc tgagcggcat cggagctgca
     6241 actgcaatat tttcacttat ttttaaggat tccatacttg gatttgtagc cggaattcag
     6301 cttaccgcaa ataatatggt gaagctgggt gattggattg aaatgccgaa atatgatgca
     6361 gacggagatg taatagaaat aacccttcat actgttaagg ttcaaaattg gaataaaacc
     6421 atctctatga ttcccactta tgcattgata tcagactcat tcaagaattg gaccggaatg
     6481 caggaatttg gcggcaggag aataaaaaga catattaaca tagacttaac cagtataaaa
     6541 ttctgcacag aggaaatgtt ggaaagattg gtaaaaaccc cttacctcca ggaatttctc
     6601 agcagttaca ataatgagca cataaatggg aaacagctga ctaatattga tgctttcaga
     6661 gcctatctgg tgggatattt gaagaatcat cccgggctca acaaagaaat gacacaaatg
     6721 gtaaggcagc gtcagccgac tgaatttggt ctgcctatag aaatatatgt ttttttcgac
     6781 ggaacaaaat gggctgatta cgaagtcgtg caatcggaaa tattcagtca tgtcattgca
     6841 atggttcagg aatttgattt acggatatat cagaaccctt cgggatatga ttttagaaat
     6901 cttggcaaat gtgaatctta atatgttcgg ttcggcttcc tgcatctgca tatcatatgt
     6961 agaagttgtg tatgtttcct aattacttcc tcttggaatc agaatccacc ttaagccttt
     7021 cctgtgagag ggatacatag attgcaaggg gtaccaggat acagctcaat agtattcttg
     7081 tcgcggtatc ctgaaatgtc tgtattattg taaaaattac agctattatt ggcagcagct
     7141 gaacattact gtttttaaaa aacaaatatt tcatatccct ttctgccgat acgatactgt
     7201 tcaaggaata tatccccagt tcaaccctgt attttgacgg aataccgatc agcgtacttg
     7261 catctgcatc atcataaaca agaacattgg aattcttaag cacctcaaag gaatcaaagg
     7321 gcagcgccct taatattgcc tcatgccctc tgtgcctcac aatcagctta tccgtctcac
     7381 caatgccgag gattttcatg gttgtaggca ttatacgcac aatatcgctg ttttcatctg
     7441 taggataagg ccttcctact cttaaacata ttgattttct tttaatgaga aaagagagaa
     7501 ttccatatat aaatcgtctc catcttcccc ccaaacccat cgcttttgtt ttatcgccag
     7561 tgtcatttac ttcaaccagc tcaattttat caagctccag ctttttaaag atgcttttta
     7621 aatcatcgtc ttcaatcaga ttttgttcaa tctcataata tgagtgccga tccttgtagc
     7681 tccgttttaa aatatccttc gctgcttcgg tgagaacatc gcttcccagg tatttcagaa
     7741 aatcatttct taagattact ttcttgggaa tatttacatt cataatatgc ctttgatagt
     7801 aattaagaaa aatcttgcct tttgcggtcg tatcccttgt cacagacagc ttcccctggc
     7861 agtagtcaat gtcatcatgc ctgttataca attgatagtt tttacttaag tcaatcttat
     7921 ccgccagatc cgggcatatc atgattcttt ccgatccggc cttggggatt ccaatcggca
     7981 gggttattgc cttcttcctg tttaccagca catattttct ctcttctgcc tcattatctc
     8041 caaaaacgtt tttgtataat ttgttggtca ggtatacctt cccgggcaca aattcctgat
     8101 tgttgcccgt atgcttgtat ctggcaatga attcggtttt gctgcagtga ttaaacacag
     8161 aattcacctg aaaggaatca tttctttgaa atccgtattt ctctgcatct tccctgctga
     8221 attctatttt atctatagga atatggattt tgcttttttc agcctcaaat acgtatgtgt
     8281 cggaatccca ttcacagtca ttcaatacgg atatgacgga atacaggcct ttcatgaaac
     8341 tcataaactc ttcctcgttt acggatactt ccctgtattt cctgttgacc tccacctgga
     8401 tagacggtat cccgcaaacc gccgccattg ttcttgatac gttgttttgc gggcttgctg
     8461 tgaacatctt attttcttca accttatgaa ttccgtattt gccgaatact acacggaaca
     8521 ggtcagggat aaagttattt ccgcaaaggt tatttgaacc atcggtcccc aggtcgatgt
     8581 caaattccct gttctccgca gctccatgca ggtctatgaa tatacgtata ttctctttcc
     8641 tgataacgtc tattacgctg ttcttataat ccccgtcccc gtttatggga tcatagttgg
     8701 ggtcatagtt gtcgtttttg gttttgtaaa acacgtggca cccggtcagc aagtgtatca
     8761 ttctggcaat tgaaccggta aatatgtctg cacattttac cttgccgttc ctcaaatgat
     8821 ttaccgaatg gggtgccgat accaaaaccg gtatggtacc cttttcatat ttataaccat
     8881 aatcaatttt tgaatccccg ttatagttat ccatggagaa tgcccgttca aacaagaggg
     8941 tctttttaac tatgtattcg cggcttttat tgttaatcat acgtacctca caaatcaatt
     9001 tacttctaaa gattcagcgt atcctgctta aaatactcct ttacatctct gaccagggac
     9061 tgtgttccta ttatgcacac cagcccgtca tccccaatca gattatatgc cgtttctatt
     9121 gcatcttcaa tctcagcttc atatatcagc ttttctccca gcataggctt tgctttgtcc
     9181 atttgagctg ttgtaaattt caaatgcttg ttctttgaag cggtgaaaat aatccttctt
     9241 gatatgtcat acatttcaca tgcaacgcct tcataatcct tatcatcggg aatcccgaca
     9301 atggagatta cgtctttatt tcccatttca cttacaaact ccctgacatg tatcgcgcac
     9361 tccctgttga tacacccgtc aagtatggtt aaagggcttc tgtttatcac ttccaacctt
     9421 cccggccatg ttatgccgtc aaatgcctgt cttatattct cttctttgac tccccccagc
     9481 aaattctctg ctgtgcaaac cgcaattgcg gcattatatg cctgatggcg tcccatcagg
     9541 ttcagcctga tatttctgta tttgttgttt cgggtaatga tatcaaaata agtgccgttt
     9601 ctgtcaacgc atattttttc acacaaaaaa tcctgtccga aggtaaataa tctgactccc
     9661 ttatcacagg cttctctaca gataacatcc cggacttcct tctgctgtac ggcactaact
     9721 gcaaacctct ggatgctgtt gattattcct gccttattaa aggctatctc cggaagatta
     9781 tatcccagct gcggaatgtg ttccggaaaa atagtgttga tgaccgaagc ttcgctatat
     9841 atccttgtta catcgtcata tcttgctcct tttccgcatt cgaacacgtt gtaatcagtc
     9901 ttttgatcca gataatacag catggcaata acggcagtaa taccggcagg ccctatatat
     9961 tgggcctctg tcaattgttt ttgcacctcc gctgcataag gctccagaat attggcatac
    10021 ttaacaaaat ccccctctga aattgcattg ccgtttattc tgattcgctc caaaaagctt
    10081 accagatgcg ggcttgtaca caatccggcc ttaagcccgt gagcctctat cagttttgat
    10141 atcattctgc tgacggagcc tttccccttg ctccctgtca ccagtatatt cctttgtttt
    10201 ttgtccggtt cttccgtttt atccagaagc gctcttgtca gatgcgggtt tctggtcacc
    10261 atatccggca tatcctttgg tatcttatct atcgcgttca tgtaggaact atatacgaaa
    10321 tcctccgctt ctttagcatt tgtaaaaata gcaaccactc ctatcatatg ttgtgcaatt
    10381 taagaagaat gcgacgggga cacggggacg tttcggggac atggggacgt ttcgcgtgtc
    10441 ccactggaag tgggacaggg gaaacgtccc cgtgtccccg cccccatgtc cccgccccca
    10501 tgtccccgtc gcattattct tcttatgcaa ctcattcaat tattattaat tattacatta
    10561 tggttttatt tttaatctcc tcaacatatc aattaaataa caatactttg tcctttaata
    10621 taacgtaggg gggataaaaa gtgttcaaca aaagaattct attcttttgc accgttcttg
    10681 ccctgctggc agcagcactt caggctgtgc ccctcatttt cggagcagca tttgcttttg
    10741 gagcattatt gaatggattt cctatatatt tagcggcacg gctcagctat ataccgggaa
    10801 tacttgttta tctttcagcc gcagccctat tggctttttt aaatcccttc gaagcccttt
    10861 ttttcatctg taccaacggc ataatagggc tttccctggg cattattaaa gaccgcttca
    10921 ggagtatcta tcctgtccct gcactttctg cactgttgga tgtcgcaata ctgttcacag
    10981 tcaattacag gttcggcata agcattttca gcccctctgc ctttagcacc cctgccggtc
    11041 aagctgccgc ccttctgttt cccatgtatt tgtattgctt gatatacctg aagctggcaa
    11101 tgtctttgga taagcttatt cacaggtaca tagaactgga tatccatcca tgacttttat
    11161 cggataaaag ccagaggatc actttccgta agcgactcag gatttatata cagagttcct
    11221 attgtagtgg accaatgcag atgcgggcct gttgaaaagc ctgttgatcc cacctctcct
    11281 atgatgtcgc cttttttaac ttcagcccct tcctccacaa gaagcttgtc aagatgggca
    11341 taggagctgt agatattgca gccatggtca attattattg tattcccagt aacgttaagc
    11401 atcatggata gccttaccac tccattattg gctgccttca caggagtccc cctggctgct
    11461 gctatgtcga tccccgagtg ccttccggac tcttccttgt taatgtatct tatcatacca
    11521 aattcggtag aaacccttcc ttccaccggt tttacaaagg tgccctccca taggggtttt
    11581 tccgaagtta cggattttgc tctttcggta tgaaccttat ccatatcaaa attatcatca
    11641 cttctctgtt ccttctgagt ggatgtaaca gtaagatgct gctccacaaa ctctttatgg
    11701 aataccttaa aggtttcctc tttttcagct gcaatttccc catccctcag aacctgaacc
    11761 ttgcagctgt aatccccttc ttttgtcctg cagcttgttc cgattatagc caaccttccc
    11821 tccataaagc tgaaaaacct tggctgcccc tgcacaaaag cagtggatac ctgaatatca
    11881 tcccttttcg tcagaccctt gagatatatt gcgaagcagt ctccgggcag cagttccgtc
    11941 tctgagaata cgatttccgg cacaggctcc ggtacaggct ccggttccgg ctccgttacc
    12001 ggctccgcct tctcgggcac aaaaaaatcc tcctgcaagc ctatgacacc gcaaacagaa
    12061 aaaaacagaa tacaaaatat aagcgctttt ataaaatatc tacccatccc aaactactcc
    12121 ttgcagcctg taaagataca ggcagccata ccttttgaac ggctgcctgt ttctcatatt
    12181 acatttttac aaaacccctt ctgcatacaa cccatcaaaa ttaagcaata tatacatgct
    12241 cccgtcatct ttctttccta caccgtttat atacttgctg ccgatatttg atacgacttc
    12301 cgacacgggt tctattttct ctctttccac cgtaattacg tttgatgctt catcgacaac
    12361 gaaaccggct atagtgcctc cggtatcggc cacgattatc tttgtatttt cttccgtgct
    12421 cttatcttca agctgaaacc tcttcttgag gcttattaca ggaatgacat tgcccctcag
    12481 attaagcacc ccttcaacat aatcgggggt attgggtatc attgtgggct tcctgtaagg
    12541 aacaatttcg ttaaccttaa atatgtctat ggcgtattcc tgctcattca acctgaataa
    12601 aaccaactgc atcatatcca ttaaagcacc ccctttttaa atcaaccgga tgttcttata
    12661 acttatattt cgacgttttt acgggaattc cttcttatct gcataatagg atgggggaca
    12721 tggggacgtt tctcc
//
LOCUS       DAOQUR010000063        12624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5635_length_12624_cov_10.098019, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000063 DAOQUR010000000
VERSION     DAOQUR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12624
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5635_length_12624_cov_10.098019"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKI50_10455"
     CDS             complement(<1..778)
                     /locus_tag="PKI50_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04160.1"
                     /translation="MNLNSDDINLYLNENIDRIYKKIIRIAEKNIGKEENFRIDIAIL
                     INDIFRDLEIENRINPEQEYSVTQGRIDSLYGNIIIEYKAPGKISSNNISINETFIEQ
                     VQRQIIGLSKRNKVDIEKILGVVFDGYNIIYVRHRNNICSVSKVIKVNKESIQLFLKR
                     LLSLSIEKKALTIENLLNDFSSDSERTRKIVSLLYKKSSNNKDYNRPKLLFEQWKHLF
                     REVCGYDFDTLDLKIQDLKQHYNISEDNVKIDYLIFSIQTY"
     gene            complement(1042..1359)
                     /locus_tag="PKI50_10460"
     CDS             complement(1042..1359)
                     /locus_tag="PKI50_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04161.1"
                     /translation="MFIIRNKGSGNKFTLISIIMIIAVIYIFRLISPQKEIEYPDTMH
                     YNNVKYEYAETVKGLPFMFVRKRPVSEEGFIILVRRGTDTQEEIFMYEGFRKYRRYEA
                     LRE"
     gene            complement(1435..1914)
                     /gene="ispF"
                     /locus_tag="PKI50_10465"
     CDS             complement(1435..1914)
                     /gene="ispF"
                     /locus_tag="PKI50_10465"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNT04162.1"
                     /translation="MHGFRVGNGYDVHKLAEGRKLILGGVEIPNAKGLLGHSDADVLV
                     HSIMDALLGACGESDIGRHFPDSDISYKGISSLVLLKKVKDILYSKGYRISNIDSTII
                     AEKPKLSPFVDEMKANIAAVLQLDKDCIGIKATTSEGLGFTGRGEGIAAYAAAIIYK"
     gene            complement(1924..2613)
                     /gene="ispD"
                     /locus_tag="PKI50_10470"
     CDS             complement(1924..2613)
                     /gene="ispD"
                     /locus_tag="PKI50_10470"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNT04163.1"
                     /translation="MNTAIVLAAGKGTRMNAGMNKQFMLINDKPLLAQTLAAFQSCSA
                     IDSIILVAGREELKTCKEQILDVYGFDKVDKLVSGGSERQQSVYNGILELEDDCSIVV
                     IHDGARPILPEGIIEKCIDGAKIYGAVSAGMPAKETIKILNEEGFVQYTPERGKVWVT
                     QTPQAFKRDIIEKAHKMANIKGISGTDDAFLVECMGIKVKMLEGSYENIKITTPEDII
                     LAEAIMRKTVE"
     gene            complement(2706..3827)
                     /locus_tag="PKI50_10475"
     CDS             complement(2706..3827)
                     /locus_tag="PKI50_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HNT04164.1"
                     /translation="MRKVIVWVLMILGAALGAESAYLLTNYFKVTRFLGFDLSGSGSI
                     VPVLFGGVIGGIIFYFISSWLINFGKESTTRVERMLQKIPAGEMLVGAIGLITGLVIA
                     YLISSGPIKLIPFAWLGTSLSILIYLLLGYLGVSIATKKREDILGLLIPWKRLVQKDK
                     EKQAAKVGSVMQPKILDTSVIIDGRIADICKTGFIEGPLIIPAFVLEELRHIADSSDA
                     LKRNRGRRGLDILNRIQKELDINVEIVEKDFEELTEVDTKLLKLAQYMNGKVITNDYN
                     LNKVAEFQRVPVLNINELANAVKPVVLPGEEMVVQVIKDGKEFGQGVAYLDDGTMIVV
                     DGGKRYIGDTIEVVVTSVLQTAAGRMIFVKPKSAADKVS"
     gene            complement(3981..4457)
                     /locus_tag="PKI50_10480"
     CDS             complement(3981..4457)
                     /locus_tag="PKI50_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNT04165.1"
                     /translation="MFSIGDMIYYPRHGAGIIEGIEEKEVLGEKCKYYVIRIALGDMK
                     VMVPISKTEFHGIRNITSRDRIDDIYNILSGETSCMCKSWSKRYRENEAIIRRGDVFE
                     ISELVKNLTVADRGKKLSTGEKRMLENVRQLLVSELMLMLDEPYEKIEKKVLGIIK"
     gene            4659..5054
                     /locus_tag="PKI50_10485"
     CDS             4659..5054
                     /locus_tag="PKI50_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNT04166.1"
                     /translation="MKDLLYDNFQDSVGELLVRHKSILDVLTKYQESQSRVSRAVVKA
                     VTSCGCIEINSKKQNYDNEITLQELKNYMDTHLKGSLCANCRDVIEKELGNNLFYIAA
                     LCNIMDISMYDVLLKEYEKMHTLGIYNLF"
     gene            complement(5139..6218)
                     /gene="disA"
                     /locus_tag="PKI50_10490"
     CDS             complement(5139..6218)
                     /gene="disA"
                     /locus_tag="PKI50_10490"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNT04167.1"
                     /translation="MRDEMGRDDGLQSCLRMIAPGTPLREGLESVLKAKTGGLIVVGD
                     SKEVMSLVDGGFHINSDFLPAYMYELAKMDGAIILSRDCKKILYANTQLIPDPSIPSS
                     ETGIRHRTAERVARQTGELVISISQRRNVITIYKGYLKYVLKDISIILSKANQALQTL
                     TKYKSVLDGTLANLGALEFDDIATVLDVALAVQKIEIVNRIVTEVEQYIYELGVEGKL
                     VSMQLEELVAGLKEEEVLLIRDYLPRGEENNYKEITRTLRNQSSEDLLELPFVARILG
                     LGNNINTLDLPISTRGYRILSKIPRLPMNIIENLVSDFERLQRILGASIEELDEVEGI
                     GEVRARIIKESLKRVYEQIQTERHI"
     gene            complement(6223..7587)
                     /gene="radA"
                     /locus_tag="PKI50_10495"
     CDS             complement(6223..7587)
                     /gene="radA"
                     /locus_tag="PKI50_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNT04168.1"
                     /translation="MAKIRTKFVCSECGSESPKWMGKCPECGQWNTLVEEVEQRDSQA
                     QQSFAGILSKPESLLDISEDIEERLSTGSYELDRVLGGGIVKGSLVLVGGDPGIGKST
                     LLLQIANNIGNNGKKVLYVSGEESTAQIKLRAFRMGIRSGNIFLVSENNTQNVDIYVR
                     DMKPDILVVDSIQTMYSPVITSAPGSVSQVREATLGLMKIAKQDGIATFIVGHVTKQG
                     AIAGPRVLEHMVDTVLYFEGETHMSYRVLRAVKNRYGSTNEIGIFEMGDKGLTEVPNP
                     SEMLLSGRMEGATGSCVISAMEGTRAMLVEVQALLSHTGFGVPRRNATGVDYNRVALL
                     MAVLEKRVGMQLGSSDSYVNVVGGIKLNEPAADLGIVAAIASSFRDREIDGSTVIIGE
                     VGLAGEVRAVNFAEKRVNEAAKLGFKRCIIPANNLSRLNDKSGIEVLGVSNVNNMLDI
                     IIGG"
     gene            complement(8001..10436)
                     /locus_tag="PKI50_10500"
     CDS             complement(8001..10436)
                     /locus_tag="PKI50_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNT04169.1"
                     /translation="MGLFDRFTERAQKVMVYSQEEAIRQNYNYIGTEHILLGILREGE
                     GIAAQVLKNKGVNLDTVRQQVEALVGKGQQEVGQVVGYTPRTKTILELSVEEARSLGQ
                     NYIGTEHLLLALIREGEGVAAQILAAMGLNFDSIRQEIIGLLDSEGAKGKRGEASAKG
                     SNTPNLNQYGRDLTEMAREGKLDPIIGREKEIERVIQILSRRTKNNPALIGDPGVGKT
                     AIAEGLAQKIIEGNIPEILKGKRVVTLDLSSMVAGSKYRGEFEERLKNVMNEIRESKN
                     VILFLDEMHTIIGAGGAEGAIDASNILKPALARGEIQAIGATTLDEYRKHVEKDAALE
                     RRFQPVMVGEPTKEEALLILEGLRDKYEAHHKVKITDEAIKAAVELSDRYIADRFLPD
                     KAIDLVDEAASKVRIKIFTAPPDLKKMEEELAALEKEKAEAISLQNYERAAELRDRLQ
                     HIRQKLEDGRKNWNDKASSKDSVVTEEDIASIVSGWTGIPVTKLAEEESNRLLNLESI
                     LHQRVIGQEEAVLAVSKAIRRARVGLKDPKRPVGSFIFLGPTGVGKTELSKALAEALF
                     GDENAVIRIDMSEYMEKHTVSKLIGSPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEK
                     AHPDVFNVLLQILDDGRLTDGKGRTVDFKNSVVIMTSNVGVSTIRKQAVLGFATSSNE
                     REDAYEKMKENVLEELKKTFRPEFLNRVDEIIVFHQLQEEHIRKIVDLMLTSLLVRIR
                     EMNINVEVTDKAREFLAEKGYDQTYGARPLRRAIQKMVEDQLSEEMLKGEIKPGSEVL
                     VDIEDDKLIFRNK"
     gene            complement(10515..11537)
                     /locus_tag="PKI50_10505"
     CDS             complement(10515..11537)
                     /locus_tag="PKI50_10505"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HNT04170.1"
                     /translation="MKGWVLGNGPQSDIILSSRIRLARNIADIPFPPGLSAEKAKEVV
                     DSVRNSLSKPESKLEFKEYIMQKLPALERQCFVERHLISPGLSRNCAGGAALISSDEM
                     VSIMVNEEDHIRIQTILPGLQIKKSWEMASDIDDILEQSLEYAYSENWGYLTSCPTNV
                     GTGMRASVMVHLPALNITGNMNKILQTVAHIGLTIRGLYGEGTEIVGNIFQISNQITL
                     GRAEEEIVENLTAVTKQIIDKEKEARRVLLESNRIKIEDKIWRSWGIMKNARVMSSQE
                     CMKLLSDVRLGVDLNIIGNIPTRLLNEIMIEAQTANIQKSSRKELTPDERDIIRAEMV
                     RSKLDS"
     gene            complement(11537..12049)
                     /locus_tag="PKI50_10510"
     CDS             complement(11537..12049)
                     /locus_tag="PKI50_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNT04171.1"
                     /translation="MICDECGKRPAAVHITKVENGKKSDMHLCEQCAMQKNLLSLSTS
                     FSVNDLLAGLLNSGGASHIKTEMVNDIKCNVCGLSYGKFRETGRFGCGNCYKVFGERL
                     NPLFKKVHGNTGHTGKIPNRAGGRIKVLREVEKLKQQLDEAIRNEEYEKAAAIRDKIR
                     DLNKAGKEEA"
     gene            complement(12075..>12624)
                     /locus_tag="PKI50_10515"
     CDS             complement(12075..>12624)
                     /locus_tag="PKI50_10515"
                     /inference="COORDINATES: protein motif:HMM:NF036474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HNT04172.1"
                     /translation="IFLYSTCKNDIIYYIIELRSKKVKVRKSDDMSRISDLIEKFISD
                     MIEESKGVVEIQRNEMANYFDCAPSQINYVLMTRFTTERGYFIESRRGGGGCIKITRI
                     DIDKDDYLMNMLSTSIGDSINKEGAFALAEALYKQKLIDCNTVNILKAATDDSTLSKV
                     GKPYRDNLRAEILKAVIVAALV"
BASE COUNT         3184 a         3265 c         2025 g         4150 t
ORIGIN      
        1 aataggtctg tatagagaat attaaatagt ctattttaac attatcttca ctaatattat
       61 aatgttgctt taagtcttga atttttaagt ccaatgtatc aaagtcataa ccacaaactt
      121 ctctaaataa atgcttccat tgctcgaata atagcttagg cctattataa tctttattat
      181 tggaactctt tttataaagc aaagatacaa tttttcttgt tctttcgcta tccgaactaa
      241 aatcatttag cagattttct atcgttaagg cttttttctc aatacttaaa gataataatc
      301 ttttcaaaaa taattgtatt gattctttat taacttttat aacttttgat acgctacaaa
      361 tattattcct gtgtcttaca tatataatat tatatccatc aaaaacaaca cctaagattt
      421 tttctatatc cactttattt cttttagata aacctattat ttgcctttgt acctgctcaa
      481 taaaagtttc attaattgaa atattattac ttgatatttt ccctggagct ttgtattcta
      541 taatgatatt accatatagg gaatctattc ttccttgtgt aacactatat tcttgttccg
      601 gattaattct attttcaatt tctaaatccc taaatatatc attaattaat attgctatat
      661 ctattctgaa attttcttct ttacctatat tcttttctgc tattcgtata atttttttat
      721 atatcctatc aatattctca tttaaatata agttaatatc atcactgtta agattcattt
      781 tatcaccctt acatatttta tttattagtt ctcctatatt caatcagata tgaaatattt
      841 gatttctgtg acttttttat taatatgcta tgactcctct tttatactta ccaagtcatt
      901 aatttgaatc tcccagctta ttgctatttt tctcaattat aacatgatca ttagcacaaa
      961 acaagaacat atgttcgatt ttatgaaaat cttttcgtaa tttccttttt taggatgtcg
     1021 cttcttggtt attcttatcc tctattctct taacgcctca tagcgcctgt atttacgaaa
     1081 tccttcgtac ataaagatct cttcctgagt gtcggtacct cttcggacaa gtattataaa
     1141 tccttcctcg cttaccggcc tcttcctcac gaacataaac ggaagaccct tcacagtctc
     1201 cgcatactca tattttacat tattataatg cattgtatcg ggatattcta tctccttctg
     1261 gggcgaaata agcctaaaaa tatatatgac tgcaattatc attataatcg aaataagcgt
     1321 gaatttattt ccgctgcctt tatttcttat tataaacatt ttcaggcgcc tcccttcgta
     1381 taaaatggct ggaagcgagc aagacaactc agtattgcga cgcttacgga cattttattt
     1441 atatatgatt gctgcggcat aagccgcaat tccttcacct ctgccggtaa aacccaaacc
     1501 ttccgaagtg gtggccttga tacctatgca atccttgtca agctgcagca ctgctgctat
     1561 atttgccttc atttcatcta caaacggcga aagttttggt ttttcagcta ttattgtact
     1621 gtcaatattt gaaatccgat atcccttgga ataaagaata tccttgactt ttttcaatag
     1681 aacaaggctt gatatccctt tataggatat atcactgtca ggaaagtgcc tgcctatatc
     1741 cgattccccg caggcaccca ataatgcatc cattattgaa tgcacaagca catctgcgtc
     1801 cgaatgcccc aacaggcctt tcgcattagg aatctctacg cctcccagta tcagcttcct
     1861 tccctctgcc agcttatgta cgtcgtagcc gtttccaacc ctgaatccat gcatgctttc
     1921 acctcattcc acagtttttc tcattatcgc ttcagcaaga attatgtcct caggagttgt
     1981 aatcttgata ttctcatagc ttccctccag catttttacc tttatcccca tacactcaac
     2041 cagaaacgca tcgtcagtgc ctgatatgcc tttgatattg gccattttgt gggctttctc
     2101 aattatatcc ctcttaaaag cctgaggcgt ctgggtaacc catacctttc cccgctcagg
     2161 cgtatattgc acaaaaccct cttcattcaa tatctttata gtttcttttg ccggcatacc
     2221 ggcggaaacc gctccgtaaa tttttgcacc gtcaatgcac ttttcaataa ttccctccgg
     2281 caatataggc ctggctccgt catgaattac aactatgctg caatcatctt ccagttccag
     2341 tataccattg taaaccgact gctggcgctc actgccccct gagaccagct tgtccacttt
     2401 atcgaagcca tagacatcaa gtatttgctc cttgcaggtc ttaagctcct ccctgcctgc
     2461 aacaagtatg atgctgtcta ttgcactgca gctttgaaaa gccgccagag tctgtgcaag
     2521 aaggggctta tcatttatca gcataaactg cttgttcatt cctgcattca ttctggtccc
     2581 tttgcctgca gcaagtacaa ttgcagtatt catagccctt caatccccca ttttttaata
     2641 aatataccct aagcggactt tatttctgct tagggtaaaa caaatgcggc ttgcgccgca
     2701 atgttttacg atactttatc tgcggcggat tttggcttta caaatatcat ccttccggct
     2761 gcagtttgca gaacgcttgt cactacaacc tcaatagtgt ctccaatgta ccgttttccg
     2821 ccatctacaa ctatcattgt cccgtcatca agatatgcga ctccttggcc aaactccttg
     2881 ccgtctttta ttacctgcac taccatttct tctcccggaa gtacaacagg cttcaccgcg
     2941 tttgcaagct cattgatatt caaaacaggc accctttgga attccgccac tttattcaga
     3001 ttgtaatcgt ttgttattac cttgccgttc atatactgag ccagcttcaa aagcttggta
     3061 tcaacctcgg tcagttcttc aaaatccttt tccactattt ctacgtttat atccagttcc
     3121 ttttgaatcc tgtttagtat atccaaacct ctgcggcccc tgtttctttt taatgcatcc
     3181 gatgaatccg caatatgtct gagttcctcc aggacaaatg caggtatgat aagcgggcct
     3241 tcaataaacc ctgtcttaca tatatccgct attcttccat ctatgataac acttgtatcg
     3301 agaattttgg gctgcataac gcttccaacc tttgcagcct gcttttcctt gtccttctgc
     3361 actaatcttt tccaaggaat aagcaatcct aatatgtcct cccttttttt agttgctata
     3421 cttactccca ggtatcccag aagcaaatat atcagtattg acaacgaagt gcccaaccat
     3481 gcaaaaggta taagctttat aggaccgctg cttataagat aagcaataac aagccctgta
     3541 atcagcccta tcgctcccac cagcatttcg ccggctggta ttttttgaag cattctctca
     3601 actcttgtgg tagactcctt accaaaatta atcagccagg aagatataaa ataaaatata
     3661 attccaccaa taaccccgcc gaataaaacc ggcacaatag aacctgaacc tgaaaggtca
     3721 aaaccgagaa accgtgtcac tttaaaataa ttcgtaagaa gatatgccga ctccgcaccc
     3781 agagcggcac ccagaatcat taacacccaa acaataactt ttcgcattta caacttcacc
     3841 tccttttatc tagtatatcc agtttgatac tttttaagct ataataaacc aattataaga
     3901 ccaattatat tataacaaat aaatctgcat taaaaaataa caattgccta tttatttaaa
     3961 ccaaatatat aaataattta ctattttata atgcccagca cctttttctc tattttctca
     4021 tagggctcat ccaacataag cataagctcg cttaccagca gctgccttac gttttcaagc
     4081 atccttttct cccctgtgga caattttttc ccgcgatctg caaccgtaag gttttttaca
     4141 agttcagata tctcgaaaac atcgcccctc cttattattg cctcattctc cctgtacctc
     4201 ttgctccagc tcttgcacat acagcttgtt tctcccgaca gtatattata tatatcgtca
     4261 attctgtccc tggatgttat atttctgatg ccatgaaatt ccgtcttgct tatgggcacc
     4321 atcaccttca tatctcccaa tgcaattctt ataacataat acttgcattt ttcaccaaga
     4381 acttcttttt cctcaatacc ttcaattatc cctgccccat gacggggata atatatcata
     4441 tcacctatac taaacattga tttgcctcca gtcgattttt acaatcttac ttgatttcca
     4501 taatcttcta tagccgatag aatgtccaca ttgcaattat aaattgacat tggaattcct
     4561 tatacttata atggatatat aacaaagctg aaacagcagc tgacaatatc agtttgatac
     4621 tatattttat gtttactttt atataggagt gatacctgat gaaagatttg ctttatgata
     4681 acttccagga cagcgttggt gaactcttgg tacgccacaa aagtattctt gatgtgctga
     4741 cgaaatatca ggagtcgcag tcgagagtga gcagagctgt ggttaaggcg gtaacctcct
     4801 gcggatgtat agaaatcaac tcgaaaaaac agaactacga taatgagata acgctgcagg
     4861 agctgaagaa ttatatggat acccatctta agggaagcct ttgtgcgaat tgcagagatg
     4921 ttattgaaaa agagctgggc aataatcttt tctatattgc agctctatgc aacataatgg
     4981 atatcagcat gtatgacgta cttctgaagg aatatgaaaa aatgcatacc cttggaattt
     5041 acaatttgtt ttaaataaat aaatgagaca gggggacgtt tctcttgtcc cgcaaacggg
     5101 acaagagaaa cgtccccctg tctcatcccc ctgtctcatt atatatgcct ttcggtctgt
     5161 atctgctcat atactctctt gaggctttcc ttgattatcc tggccctgac ctctcctatg
     5221 ccctccactt catccagctc ctcgatggaa gccccaagaa tcctttgcag cctttcaaaa
     5281 tcagatacca ggttttcaat tatgttcatg ggcagccttg gaattttact cagtatccta
     5341 tatcctcttg ttgatatcgg aaggtctaaa gtattgatat tattccccaa tcccaatatc
     5401 ctggctacaa aaggcaactc aagaaggtcc tctgaagact ggttcctcaa ggttctggtt
     5461 atttccttat aattattttc ctctcccctg ggcagatagt ccctgatcag gagtacttcc
     5521 tcctccttca ggcctgcaac aagttcttcc agctgcatac tgacaagctt tccttcaacg
     5581 cccagttcat atatgtattg ttcaacctca gtcacaatcc tgtttacaat ctcaattttc
     5641 tgtactgcca gcgctacatc cagcacagtt gcaatatcat caaactctaa agccccaaga
     5701 ttcgccaagg ttccgtccag caccgattta tacttggtca gggtctgcag cgcctgattg
     5761 gctttggaca gtattatact tatatcctta agaacatatt tcagatatcc tttataaata
     5821 gttatgacgt tccttctttg agaaatagaa ataaccaatt ccccggtctg ccgtgcaacc
     5881 ctctccgctg ttctgtgcct gataccggtt tcggaagatg gtattgaagg atcgggaatc
     5941 aattgagtat tggcatatag tattttcttg caatccctgc tgagtatgat tgcaccgtcc
     6001 atctttgcca actcgtacat gtacgccggc aagaagtccg aattgatatg aaagcctcca
     6061 tctacaaggg acataacctc cttactgtca ccaaccacaa tgagtccgcc tgttttcgcc
     6121 ttcagtacgc tttccaggcc ttccctcaga ggagtgccgg gagcaatcat cctgaggcag
     6181 ctctgaaggc cgtcatccct tcccatttca tctctcatat gcctatcctc ctattattat
     6241 gtcaagcata ttatttacat ttgacacccc aagtacctct atgcctgatt tatcattaag
     6301 cctgcttaaa ttgttcgcgg gtattatgca tcttttaaaa ccaagcttcg cagcttcgtt
     6361 taccctcttc tcggcaaagt tcaccgctct tacttctccg gcaagcccta cttctccaat
     6421 aattacagtg ctgccgtcaa tttccctgtc tctgaagctg gaggcaatag ctgcaactat
     6481 tccaaggtct gctgcaggct cgttaagctt gatccctcct actacattca catatgaatc
     6541 actgctccca agctgcatac cgactctctt ctccagtacg gccatcagca gcgcaaccct
     6601 gttgtaatca actcccgtag cattccttct tggaaccccg aaacctgtat ggctgagtaa
     6661 agcctgaacc tctaccagca ttgcccttgt accttccata gcagaaataa cacaggatcc
     6721 ggtggctccc tccattcttc cggacaaaag catctcagaa ggattgggca cctctgtaag
     6781 ccctttatct cccatttcaa atattcctat ctcatttgtg gagccgtatc tgtttttaac
     6841 agccctaagc actctgtatg acatatgggt ctctccttca aaataaagaa cagtgtcaac
     6901 catatgttca agcaccctgg ggcctgctat tgctccctgc tttgtcacat gccccacaat
     6961 aaaagtcgct ataccgtcct gttttgcaat tttcataagc ccgagggtcg cctccctgac
     7021 ttggctgaca ctgccgggag ccgatgttat tacagggctg tacattgttt gtatagaatc
     7081 caccaccaga atatcaggct tcatgtccct gacataaata tcaacattct gagtgttgtt
     7141 ttcggatacc agaaatatat tccccgatct gatgcccatt cggaatgccc tcagctttat
     7201 ctgcgcggtt gattcttctc cggatacata taagactttt ttgccgttgt ttcctatatt
     7261 gtttgcaatc tgaagcagca aggttgactt cccgattccc ggatctcctc ctacgagtac
     7321 cagggaacct ttgacaatgc cccctcccag aaccctgtcc aattcatagc tgcctgtgct
     7381 tagtctttcc tcaatatcct ccgaaatatc caacagggac tccggcttcg acagaattcc
     7441 tgcaaaactt tgctgagcct gggaatccct ctgttccact tcttcaacaa gagtattcca
     7501 ctgtccgcac tcggggcatt tgcccatcca tttgggtgat tcgcttccgc attcgctgca
     7561 tacaaatttt gttcttattt tagccattta aacacctcaa gaattatatc ggaattttca
     7621 cagattttgt aaatcaatat gagtatttcc caatcattcc aacttttttc actgaaacat
     7681 attcttatta tactatattt gacaatagaa gaataatgcg tcgcattttt cgggcacgag
     7741 gtacgaggcg cgaggtttag gtcagagctt cgaagccatt aatgccgggt aacacgggag
     7801 acacggctgt ttcacggaat tcacggaaaa ctatatattt ccccgccgtg aatttagtgg
     7861 ctttcagtgt tttcagtgtt atgcgttatt tacccgtaag gaatacccaa gcataccccg
     7921 caaccccgta acctcgaggc ctcgcaacct gaagaaaatt gctgcgcaat tttcttcttt
     7981 tgcgataccc cccggatatt ttatttattc ctgaaaatca gcttgtcatc ctcaatatcc
     8041 accaacactt ccgaacccgg ctttatttcc cccttaagca tttcctccga tagctgatcc
     8101 tctaccatct tttgtatggc acggcgcaga ggccttgccc cataagtctg gtcatagccc
     8161 ttttctgcca gaaattctct tgccttgtcg gtaacttcca cattgatatt catttccctg
     8221 attctgacaa gaagcgacgt aagcatcaaa tctacaatct ttcggatatg ttcttcctgc
     8281 agctggtgga atacgataat ttcatcgacc ctgttcagga actccggcct gaaggttttt
     8341 ttaagctctt ccaggacatt ttctttcatt ttttcatagg catcttctct ctcgttagat
     8401 gatgtggcaa acccaagaac agcctgcttt cttattgtac ttaccccaac attggaggtc
     8461 ataataacaa ctgagttttt gaagtcaacc gttcttccct tgccgtcagt cagcctgccg
     8521 tcatcgagta tctgaagcag tacattgaat acatccgggt gagccttttc tatctcatca
     8581 agaagtatga ccgaatatgg ctttcttctt accttttcag tcagctgccc cccttcctca
     8641 tagcccacat agcccggagg ggaacctata agctttgata ctgtatgctt ttccatgtat
     8701 tcggacatat caatcctgat tactgcattt tcatcaccga acaatgcttc tgccagagcc
     8761 tttgaaagct cggttttccc tactcctgtg gggcccagga atataaagga acccaccggc
     8821 cttttaggat ccttcagccc tactcttgcc cttctgattg ccttggaaac tgccaatacc
     8881 gcctcttcct gcccgataac ccgctgatgc aatattgatt ccagattaag caatctattg
     8941 gattcctcct ctgccagctt ggttacagga atcccggtcc agccggatac gatggaagca
     9001 atatcttctt cggtaacaac ggaatccttt gagctggcct tgtcattcca gttcttcctg
     9061 ccatcctcaa gcttttgcct gatatgctgc aatctgtccc gaagctctgc cgccctctca
     9121 taattctgaa ggcttattgc ttcagccttt tccttttcca aagctgcaag ctcctcctcc
     9181 atttttttaa gatccggagg cgcggtaaat atctttattc tgactttgga cgcagcttca
     9241 tccaccaggt ctatggcctt atcgggaagg aacctgtctg caatatacct gtcggacaat
     9301 tcgactgccg cctttattgc ttcatcggtt atcttaacct tatgatgggc ttcatattta
     9361 tctcttaagc cttcaagtat aagcaaagcc tcctcttttg tgggttcccc caccatcaca
     9421 ggctgaaaac gcctttccag tgccgcatcc ttctcaacat gcttgcggta ctcatcaaga
     9481 gttgtcgccc cgattgcctg aatttcacct cttgccaaag caggtttcaa tatattggaa
     9541 gcgtctatag ccccttcagc tccgcctgct ccgataatgg tatgcatttc atccagaaaa
     9601 agtatcacat tctttgattc acgtatttca ttcattacgt tcttgagtct ttcctcaaat
     9661 tctcccctgt acttggagcc ggccaccatg gaagacaaat ccaatgtaac gacccttttc
     9721 ccttttagta tttcgggaat attcccttca attattttct gggccaatcc ttctgcaatg
     9781 gctgttttgc ctaccccggg atcgccgatc agggccggat tgttttttgt ccttctgctc
     9841 aatatctgta ttacacgctc tatttctttt tctcttccta taatagggtc cagcttcccc
     9901 tccctggcca tttcagtcag atccctgccg tattgattca gattcggtgt gttggagcct
     9961 tttgccgatg cctcgccgcg cttgcccttt gccccttcgc tgtccagcag tcctattatc
    10021 tcctgcctta tactgtcgaa attcaggccc attgccgcaa gtatctgtgc cgccacacct
    10081 tcaccttcgc ggattaacgc caaaagcaga tgttcagttc cgatatagtt ttgccccaag
    10141 cttcttgcct cttctacact cagttcaaga atcgtctttg ttctcggagt atagcctaca
    10201 acctgtccaa cttcctgctg cccctttccg actaaggcct ccacctgctg tctcacagta
    10261 tccaaattaa ctcccttatt tttaagcact tgggcggcaa tgccctctcc ttcccttagg
    10321 atacccagca atatgtgttc agtgcctata tagttgtaat tctgccggat agcttcctcc
    10381 tgtgaatata ccattacctt ttgggctctc tcagtaaatc tatcaaataa tcccatatta
    10441 tttcctccta tatgtcttgc tgcgggcttc gcagcttagc tcttccgttg aagccctgtt
    10501 tgaaattccc gtatctaact atcaagtttg cttcttacca tctctgctct tattatatct
    10561 ctctcatccg gagttaattc tttcctgctg ctcttttgta tatttgccgt ttgggcctct
    10621 atcatgattt cattcaaaag ccttgtggga atattgccga taatattcaa atccaccccc
    10681 agccttacat ccgaaagcag cttcatacac tcctgcgaac tcatcactct ggcatttttc
    10741 ataatacccc aggaacgcca aattttatcc tctatcttta tcctgttgct ttccagcagt
    10801 acccttcttg cttccttttc cttgtcaatt atttgctttg ttacggcagt gagattttct
    10861 actatttcct cttccgctct tccaagggtt atttggttgg aaatctgaaa tatattaccc
    10921 acaatttctg tgccttcgcc atacaaacct cttattgtaa ggcctatgtg agctactgtc
    10981 tgaagtatct tgttcatatt gcccgttata ttgagtgccg gcagatgcac cattacagat
    11041 gctctcatac ctgttcccac gttggtaggg cagcttgtaa gatagcccca gttttcactg
    11101 tatgcgtatt caaggctctg ctccaaaata tcatctatat cactggccat ttcccaggat
    11161 ttctttattt gaagccccgg aagtattgtc tgtatcctta tatgatcctc ctcattgacc
    11221 attatgctta ccatttcgtc gctgcttata agcgccgccc ctccggcgca gttcctggaa
    11281 agccccggac ttataaggtg cctttccaca aaacactgtc tctccaaggc cggaagcttc
    11341 tgcatgatat attccttgaa ttccaatttt gattccggct tggaaagact gtttctgacg
    11401 ctatccacaa cttcttttgc tttttcagca cttaatcctg gcggaaacgg aatatccgct
    11461 atgtttctgg ccaatcttat acggctgctc aaaattatgt cgctctgagg gccgttgccc
    11521 agcacccagc ctttcatcat gcttcctcct ttcctgcctt gtttaaatcc ctaatcttat
    11581 cccttatagc tgcggctttt tcatactctt catttcgtat tgcctcatcc aactgctgtt
    11641 tgagtttttc gacctcccgc agcaccttta tcctgccgcc tgccctgttt ggtatcttgc
    11701 ctgtatggcc ggtattgcca tgaacctttt taaaaagcgg attaagcctt tcaccaaata
    11761 ctttgtagca attaccgcat ccgaatctcc cggtttccct gaacttccca taggacaatc
    11821 cgcaaacatt acatttgata tcattcacca tttcggtctt aatatgagat gctcctcctg
    11881 aattcaacaa tccggccaat aaatcattca ccgaaaagct tgtgcttaaa ctcagcaggt
    11941 tcttctgcat ggcacactgc tcacacaaat gcatatccga ctttttccca ttctcaacct
    12001 ttgttatatg aaccgctgcc ggccttttcc cgcattcatc acaaatcaaa acaataacct
    12061 cctaacgaag taatctaaac cagtgctgca acaattacag ccttcaatat ctctgccctc
    12121 aaattgtccc tgtatggttt tcccaccttg gagagggtac tgtcatcggt tgctgcctta
    12181 agaatattca cagtattaca gtcaatcagc ttctgcttgt acaaagcttc cgccagggca
    12241 aaagcccctt ccttgttgat gctgtcccct atactggtgg aaagcatatt catcagataa
    12301 tcatccttgt caatgtcaat acgggtgatc ttaatacagc ctccgccgcc cctccgggat
    12361 tctatgaaat aacctctttc ggtagtgaac cttgtcatca gcacatagtt tatctgtgat
    12421 ggggcacagt caaaataatt ggccatttca tttctctgaa tctcaacaac acccttgctc
    12481 tcttctatca tgtcacttat gaacttttca attaaatcac taatccttga catatcatca
    12541 ctctttctga ctttgacttt ctttgacctt aattctatta tataatatat aatatcattt
    12601 ttacaagtac tatataaaaa tatt
//
LOCUS       DAOQUR010000064        12565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5676_length_12565_cov_9.730536, whole genome shotgun sequence.
ACCESSION   DAOQUR010000064 DAOQUR010000000
VERSION     DAOQUR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5676_length_12565_cov_9.730536"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            435..926
                     /locus_tag="PKI50_10520"
     CDS             435..926
                     /locus_tag="PKI50_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HNT04173.1"
                     /translation="MSFEIIGISSSLIEKQAITEIERCNEFTAEFGLALSPSDAAELV
                     EARNIALKSNGRIEFGGGAIHKIIREFCDSPYISMHNYAETIHELTEMFYYYKNETLD
                     LMSDDDLIKFMKNSFDGKCQGSLELLSGRELANMARNLRYGYPPDYSEDPMPDEEDED
                     GEY"
     gene            913..2274
                     /locus_tag="PKI50_10525"
     CDS             913..2274
                     /locus_tag="PKI50_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HNT04174.1"
                     /translation="MANIDKQHVIESNKLSGEQYFTSILQGAYANGLLCDSDMENIQL
                     QCIQLLANICERYSGGESSSIRVEAAENIMKSNLYTIGLYLKSLPDVDFAVNEIKKAS
                     VSEMYQKGRKLINVRLHSAKHIYNLVRKNKLFTPNYSYNSTINEGIGSFFKAYDPDYE
                     AHETPASIDYQLCNPVTNMIGIEFILKYLENLFLENEFCRGFAPEDIHHMLYGYDKGY
                     KDLLINIFEQVLTAALGCSLANRNVVELDISAEEIRHLHYELSEYDDCSLSLKLRKAA
                     GKMLEELNADSLPLKRYIERSMPKIITNILHAVRMNTLNKTFISKALPDLKPRIRFLS
                     GTKMHDEDYRKLIDELLMCRHSPDKLALIKEKVKSFGDLEDLLFDAQLSEEETDSVFD
                     ILEDVEIAALIRRHPFRTDIQAVDLSEGEMALRQYLKNYIDRLPKGKQEEIFEMADRL
                     IDE"
     gene            2469..3350
                     /locus_tag="PKI50_10530"
     CDS             2469..3350
                     /locus_tag="PKI50_10530"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNT04175.1"
                     /translation="MKIYYFSGTGNSFAVAKDIAKKADAELISIPGAMYMDKIQVDAE
                     SIGIIFPSYLAPLSGVPLIVERFVMKIANIGSIYIFAVCTCGGYECVNALPSLERLRH
                     IIGFCGGKLSAEYSVRLPMNNLDYDHIPIPIIRDQEIIINKSKAKIDDVCYRIINKRG
                     TKYKIVKKLFGLLMTPLYKLIRQSVINDLKEKAKEPMDTGLKYFELIPLTDKSIIIDE
                     KCNGCGTCEKVCPVNNIKIVDKKPEFQHKCEMCFACDEWCSYNAIHHWSRTVGVKYHH
                     PEVEISDMLCKKVLDGN"
     gene            3461..5209
                     /locus_tag="PKI50_10535"
     CDS             3461..5209
                     /locus_tag="PKI50_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04176.1"
                     /translation="MSKIRRFISYYKPYKGLFFLDMFCSLTVAGIDLLFPVLVRYLLD
                     HGINVEIGVAFDIILKIGALMLGMHILKYFCQYFITAWGHIMGARIEYDMREEIFSHL
                     QKLSFSFYDNNKTGHIMSRIVNDLFDISELAHHGPEEVFISIIKILGAFLILLRINMK
                     LTLVTFAFVPLMMGFAYFYNNKMRSVFRRNRQKVAEINAQLEDSIAGIRVVQSFSNER
                     EEQEKFTRGNRGFLDTKQESYFYMGRFFSGIEFFDGIIYIFAVIAGAYFIKLGTAATS
                     DLVAYLLYINTFLVPIRSLIHFTEQFQKGMTGFERFLEILDTEPDIKDGEDSISLEKV
                     EGRVEFTDVSFKYGEGGQVLKDINLNLEPGETVALVGPSGGGKSTLCSLIPRFYEASE
                     GIIKIDGVDIKNIKLKSLRKNIGIVQQDVYLFAGTVTENIKYGKPGASDEEVIAAAKK
                     ANAHDFIMTLEKGYDTYVGERGVKLSGGQKQRISIARAFLKNPPILILDEATSSLDNQ
                     SERIVQQSLEELSSSRTTFVIAHRLSTIKRAKTILVLTDNGIEEKGNHEELISSGGVY
                     ASLYNAQFEDDMEMEI"
     gene            complement(5139..5792)
                     /locus_tag="PKI50_10540"
     CDS             complement(5139..5792)
                     /locus_tag="PKI50_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT04177.1"
                     /translation="MINLLNETISYVAGTLLHNAPVLVFGILTAAAVKVYADQEKLRK
                     VLIKKAGSFAEKYKLKELFKVFYEVGIKQVILYFAIFAAIGFLINRFVPAEVITKYLG
                     TDNKFAVPLMALVGLPLFVSGSSSIPILNTLIAGGASQGALLAFMITGPGTSAGVIAG
                     LAVIMKRKAIGLYIAYFLAFGILLGYLYDFLLMLVISPFPYHPQTAHYTGMRILRRC"
     gene            complement(5854..6180)
                     /locus_tag="PKI50_10545"
     CDS             complement(5854..6180)
                     /locus_tag="PKI50_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT04178.1"
                     /translation="MNISRICNALGNEVRYQIVKSLKNRSIATCCNRIEYYENGISVG
                     DVVNATGLAQSTVSQHIKVLLDAGIIYKEKRGQWTCFFLNGEIINNFIKGLSHDLIQT
                     ECDCKK"
     gene            6401..6736
                     /locus_tag="PKI50_10550"
     CDS             6401..6736
                     /locus_tag="PKI50_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT04179.1"
                     /translation="MNLDYNENAKIIKALSDPSRLKIIDILSCGEMCACDILKHFDFT
                     QPTLSHHMKVLMECGIVECRKEGLWNYYSLNGTNCNKLILFLMSIITNTDDCICKEKP
                     KCGCECEGK"
     gene            6737..7801
                     /gene="arsB"
                     /locus_tag="PKI50_10555"
     CDS             6737..7801
                     /gene="arsB"
                     /locus_tag="PKI50_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNT04180.1"
                     /translation="MNNKQKSIERDNEALGFFAKYLTVWVAICIAAGVILGQVLPIVP
                     ETLSKFEYANVSIPVAILIWLMIYPMMLKIDFSSIVNATKKPKGLIVTCGTNWLIKPF
                     TMYAIAALFFHVVFKALIPAELAKEYLAGAVLLGAAPCTAMVFVWSHLTKGDPAYTLV
                     QVAVNDLILLAAFTPIVAFLLGVSDVAVPYDTLFLSVILFVVIPLAGGYFSRKYIIKN
                     KGLDYFNNVFLKMFDNTTVIGLLLTLIIIFSFQGEIILNNPLHIALIAVPLTIQTFLI
                     FFLAYGWAKAWKLKHDIAAPAGMIGASNFFELAVAVAISLFGLESGAALATVVGVLVE
                     VPVMLALVKIANSTRHWFAE"
     gene            7968..8984
                     /locus_tag="PKI50_10560"
                     /pseudo
     CDS             7968..8984
                     /locus_tag="PKI50_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            9011..9241
                     /locus_tag="PKI50_10565"
     CDS             9011..9241
                     /locus_tag="PKI50_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT04181.1"
                     /translation="MVIKVLGSGCMNCRKLEENTKAAVKELGIDAKVEKVENYKDILA
                     YGVMKTPALVVNEEVKVMGRVADTEEIKKILR"
     gene            9405..9533
                     /locus_tag="PKI50_10570"
     CDS             9405..9533
                     /locus_tag="PKI50_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LDCC motif putative metal-binding protein"
                     /protein_id="HNT04182.1"
                     /translation="MKNLKKIIDDFLKRLAAQNKEQFGSEKLDCCNLDRPKNDSRE"
     gene            9551..10942
                     /locus_tag="PKI50_10575"
     CDS             9551..10942
                     /locus_tag="PKI50_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT04183.1"
                     /translation="MKVSLKTKLTLSHVATALICVALISLFSNILLERHFQDYIRSNQ
                     EKKNKELVSLFSSNYQSNGQWNAESVQDIGIYALEQGMIVEVRDQSGNIIWNAMSYNN
                     GWCNQMLDHMANNMYSRYPNWEGKLVQVNYPVFYKGEKVGSIDIGYYGPFYFNDNDMA
                     FISTLNRIFISVGVFSLLLAFLFGSIMSRRITMPITRVINTARMIAKGYFGDRSFERT
                     STKEISQLTDTINDLAETLETQEKLRKRLTGDVAHELRTPVATLQSHLEAMLDGIWEP
                     SSDRLKSCHEETMRINRMIGDLEKLARYESDNMILNEEEFSLCELIKIMVQNFEKEFI
                     QRNIKIQIQCENIMLFADRDKISQVLVNLISNALKYTNSNGQVTITASDSGDNTTIAV
                     RDTGIGISEEDLPYIFERFYRSDKSRNRTTGGSGIGLTIAKAIVEAHKGSISASGKLN
                     EGTVFTVALPKKR"
     gene            11147..11710
                     /locus_tag="PKI50_10580"
     CDS             11147..11710
                     /locus_tag="PKI50_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT04184.1"
                     /translation="MKNKSWIIIIGIVVLLAVYVVGVQRSLVNLSEEVNASWSDIEVQ
                     YQRRADLIPNIVETVKAQAIKEQEIFTQIAEARSKLSGAANIQEKIDANAEVSSVLGR
                     LLAIAENYPELKQNQGFQDLRVQLEGTENRIAVARKYYNDNVKEYNKKIKVFPTNIAA
                     GILGYSDEFPYIEAEAGAEQAPDVNFD"
     gene            11802..>12565
                     /locus_tag="PKI50_10585"
     CDS             11802..>12565
                     /locus_tag="PKI50_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNT04185.1"
                     /translation="MKQLRLSAAFIAVLLLIVTSFGVIIVQGAEPEIPNPTREFYVND
                     FAGILDSGTERDILALGENTYKATDGGQVVFVSIDTLNGNTIEEYANTLFNKWKIGTE
                     DKGILFILSMKERESRIEVGYGYEGVLTDLESSKLLVKFAGLYEEKGMDEAVKTIYSD
                     IANIVSGNEDMVRYPEIGEERQYTSDDFSFFRENPIITVILVIIILILIVLDFIFTGG
                     RVTFFIIRMAASSGRRGGGGGRNSGGGGRSGGGGASG"
BASE COUNT         3893 a         2159 c         2895 g         3618 t
ORIGIN      
        1 aaggctgctg aatataatgc cagggtttta cttatgttta atccgatgca ttatattcag
       61 tttttgtttt atgtatgatg agcaataaaa aatgcgtctt acgacacatt tttttcgcta
      121 gggaacccta tggttcccga aggcgttagc aagcatcctg agcaccctcc ttggacggca
      181 gggggtttct cccccttgca tcccccatcg tataggaaat tattatttcc tatacgataa
      241 aaaaagcgag tataaatatc gctcctatta tagtaagtat attgcttttt gcatttaaaa
      301 gtggtatatt ataagatacg gttaatttat attagcatac aatacaccca ttatactata
      361 tcacattaca gtcaatatgt caatagcttt ttggaagttt ttcagaataa aattttatat
      421 tggggtgatt gcttttgtca tttgagatta ttggcatatc ttcgtcgctg atagaaaagc
      481 aagcgataac agagattgaa agatgcaacg aattcacagc cgaattcggg ttagccctat
      541 ccccctccga tgcagctgaa ctggttgaag cccgtaatat tgccctgaaa agcaacggaa
      601 ggatcgaatt cgggggcggt gccatccata agataatcag ggaattctgt gactccccgt
      661 atatttcaat gcacaactat gctgaaacaa ttcatgagct tacggagatg ttttactatt
      721 acaaaaatga aaccctagat ttgatgagcg atgatgatct gataaaattc atgaaaaaca
      781 gctttgatgg caaatgccag ggatccctgg aactgctttc cggacgggag cttgccaata
      841 tggcgcgtaa tttgagatac ggttatcctc cggattattc ggaggatccg atgccggatg
      901 aggaggatga agatggcgaa tattgataag caacatgtca ttgaaagtaa taagctgagc
      961 ggtgagcaat attttacttc aatcctgcaa ggggcatatg ccaatgggct tttatgcgac
     1021 tccgacatgg agaacattca gctgcaatgc atacagctat tggcaaatat atgtgaaaga
     1081 tacagcggcg gtgaaagcag ttccataaga gtggaggctg ctgaaaacat tatgaaatcc
     1141 aacctctaca caataggact atatttgaag tcgctgcccg atgttgactt cgctgtcaat
     1201 gaaataaaaa aggcaagtgt ctccgaaatg tatcagaagg gcagaaaact gattaatgtc
     1261 aggcttcatt ccgcaaaaca tatttataat ctggttcgga agaataaatt gtttacaccc
     1321 aactattcct ataactcaac aataaacgaa ggtatcggaa gcttcttcaa ggcatatgac
     1381 cccgattatg aggcccatga gactcccgca tccatcgatt atcagctgtg taatcctgtc
     1441 actaatatga tagggatcga atttatcctt aaataccttg agaatctttt tctggaaaat
     1501 gaattctgca ggggttttgc cccagaggat atccatcata tgctttacgg atacgacaaa
     1561 gggtataagg atttactgat taatatcttt gagcaggtat tgaccgcggc acttgggtgc
     1621 tcccttgcaa accgaaacgt tgtggaactg gatatttcag cagaagaaat ccggcatctt
     1681 cattatgaat tatcggaata cgacgattgt tcactttctt tgaagctccg caaagcagca
     1741 ggaaagatgc ttgaagagct gaatgctgac agtcttcccc taaaaagata tatagaaaga
     1801 agcatgccga aaattataac caatatcctt cacgcagtaa ggatgaatac tctgaacaaa
     1861 accttcatat cgaaggcttt gccggacttg aaacccagaa tccgttttct gtccgggacg
     1921 aagatgcatg acgaggatta cagaaagctg attgacgaat tgctcatgtg ccgccattca
     1981 cccgataagc ttgcgcttat aaaagaaaag gtaaaatcct tcggagactt ggaagattta
     2041 ttgtttgacg cacagctcag tgaagaagaa acggattcgg tttttgatat tcttgaagat
     2101 gtagagattg ccgcattgat aagaaggcat ccgtttagaa ctgatataca ggctgtggat
     2161 ttatcggaag gggaaatggc attgcggcaa tatttgaaaa actatattga ccggcttccg
     2221 aaaggtaagc aggaagagat ttttgaaatg gcggataggc ttatcgatga ataagcattc
     2281 cgatgaggac atggggacgg gggacatggg gacgtttccc ctgtctcact tccagtgaga
     2341 cacgcgaaac gtccccgtgt cccccgtccc catgtccttc tattgaaata agattgcagg
     2401 aaatatataa tataattgat aatggtattt tttgatcaat aaggttcaag gaggttccgg
     2461 caataagtat gaaaatatac tatttctcag gaacaggaaa ctcatttgct gtggctaagg
     2521 atattgcaaa gaaagcggat gcggagttga tatctatccc gggtgccatg tacatggaca
     2581 aaatacaagt tgatgccgaa agtatcggaa ttatctttcc ttcatatttg gctccattat
     2641 cgggagtgcc ccttatagta gagcgatttg taatgaaaat agccaatatt ggatcaatat
     2701 atatttttgc ggtttgtact tgcggaggat atgaatgcgt taatgcactt ccttcgttag
     2761 aaagattgag acatataata ggattctgcg gcggaaaact gtcggctgag tattccgtaa
     2821 gacttcctat gaacaacttg gattacgatc atattcctat tcccataatc agggatcaag
     2881 aaattataat caataagagc aaagctaaaa ttgacgatgt ttgttatcgc atcataaata
     2941 aaagaggcac caaatataaa attgtaaaaa aattgtttgg cctgctgatg acaccgttat
     3001 ataaactgat acggcagtca gtaataaatg acttgaagga aaaagcaaaa gagccgatgg
     3061 atacgggatt aaaatacttt gaattgattc cattaacaga caaaagcatt attatagatg
     3121 aaaaatgcaa cggctgcgga acatgcgaaa aggtatgccc tgtaaataat attaaaattg
     3181 ttgataaaaa accggaattt cagcacaagt gcgaaatgtg ctttgcatgt gacgaatggt
     3241 gttcgtacaa tgcgatacat cattggagca gaactgtagg ggtgaagtat catcatcctg
     3301 aggttgaaat ctctgatatg ctttgcaaaa aagtgttgga tggaaattaa gcagtataaa
     3361 tatttgttgg aatacatacg gcaaatgcat ataatatatt tgttaacaat gtttttttac
     3421 ttagtgcagt tttattgttt tgttgtgggg gttttaacct atgagtaaga taaggagatt
     3481 catttcatat tataagccct ataaaggctt attctttctt gatatgttct gttcactcac
     3541 ggtagcggga atagatttgc ttttccccgt gcttgtaaga tacctgctgg atcatggtat
     3601 aaatgttgaa atcggtgtgg cttttgatat tattttaaag attggggcat taatgctcgg
     3661 gatgcacata ctcaagtact tctgtcagta ttttatcacg gcttggggac atatcatggg
     3721 tgccagaatt gaatatgaca tgagagaaga aattttttcg catctccaga aactgtcttt
     3781 ttccttctat gacaataaca agaccgggca tataatgtcg agaatcgtca acgatctgtt
     3841 tgatataagc gaattggcac accacgggcc tgaggaagta ttcatttcaa ttataaaaat
     3901 actcggagct ttccttatac tgctcaggat taatatgaag cttaccttgg ttacctttgc
     3961 ttttgtaccc ttaatgatgg gctttgcata tttctacaac aacaagatgc gctctgtttt
     4021 caggaggaac aggcagaagg tggcggaaat aaatgcccag cttgaggaca gcatcgccgg
     4081 aatcagagtt gtacagtcct tttccaatga aagagaggaa caggagaagt tcacccgggg
     4141 gaacaggggc tttcttgaca caaaacagga aagttacttt tatatgggcc gcttcttcag
     4201 cgggatagaa ttttttgacg gtataattta tatttttgcc gttattgccg gtgcatattt
     4261 cataaagctt ggaacagcag caacctcgga tctcgtggca tatctgctct acattaatac
     4321 cttccttgtg cccataaggt cccttatcca ttttaccgag cagttccaaa aggggatgac
     4381 gggatttgaa aggtttctgg aaatattgga tacggaaccg gacataaagg acggtgagga
     4441 tagcatatcc cttgagaaag ttgagggaag ggttgaattt acagatgttt ccttcaagta
     4501 tggcgaagga ggccaggtat taaaggatat aaatctgaat cttgagcccg gagagacggt
     4561 tgctcttgtg ggaccctcgg ggggaggaaa atcaacactc tgcagcctta taccaaggtt
     4621 ttatgaggct tctgagggta ttataaaaat tgacggagta gacattaaga acatcaaact
     4681 gaagtccctg agaaaaaata tcgggattgt ccagcaggac gtttacctat ttgcggggac
     4741 tgtaacggaa aacataaaat acggcaagcc cggggcctcg gatgaagaag ttatagcggc
     4801 agcaaaaaag gctaatgccc acgactttat aatgactctg gaaaagggct atgataccta
     4861 tgtgggagaa aggggagtca agctttccgg agggcagaag cagagaataa gcattgccag
     4921 ggcttttctg aagaaccccc ctatactcat tctggatgaa gcaacctcat ccctggataa
     4981 ccaaagtgag aggatagtgc agcaatcctt ggaggaattg tccagcagca gaaccacttt
     5041 tgtgatagcc cacaggctgt ctacaatcaa aagggcaaag acaatactgg tgcttaccga
     5101 taatgggata gaagaaaagg gaaaccatga ggagcttatt agcagcggcg gagtatacgc
     5161 atccctgtat aatgcgcagt ttgaggatga tatggaaatg gagatataac aagcattaat
     5221 aggaaatcgt aaaggtatcc aaggagtatt ccaaatgcca gaaagtaagc aatatataat
     5281 cctattgcct ttcttttcat aatcacagct aaacccgcaa tcacccctgc gcttgtaccg
     5341 ggacccgtta tcatgaaagc cagaagtgct ccttggctgg cacctcctgc tataagagta
     5401 tttaatatgg gaatggagga tgaaccgctt acaaataggg gtaatcctac aagagccatt
     5461 aatggtactg caaatttgtt gtctgttccc aggtatttgg taattacttc tgccggtacg
     5521 aatctgttta tcaggaaacc tattgcggcg aatatggcaa agtataatat aacttgcttt
     5581 atacccactt catagaatac tttaaaaagt tcctttaatt tgtatttttc tgcaaagctc
     5641 ccggcttttt ttataagcac ttttcttagt ttttcctggt ctgcatatac ttttacggct
     5701 gcagctgtaa gtataccgaa gacaaggacc ggagcattat ggagcaatgt gccggcaaca
     5761 tagctgatgg tttcgtttaa aagattaatc attatacttc ctccaaaatt aatcgtaaat
     5821 ttacgatata tttatttaaa aaaaattcaa ccgttatttt ttgcagtcgc attcagtctg
     5881 gatcaagtca tgtgaaagtc ctttaatgaa gttattgatt atttctccgt ttaagaagaa
     5941 gcaggtccac tgtcctcttt tctccttgta aatgattccg gcatccagaa gcacttttat
     6001 atgctgtgat accgttgatt gtgcgagccc tgtagcattt accacatcac cgacgcttat
     6061 gccgttttca tagtattcaa ttctgttaca gcaagtagct attgatctgt ttttcagtga
     6121 tttgactatc tgatatctga cttcgtttcc taaagcatta cagattcttg atatattcat
     6181 ataatcaacc cctatatcgt ttttctacga tatatcgtat cgtaaattta cgatggtatc
     6241 aataggaaac tataaatttt tttgtattgt gtatggtttt atatagagga ttgcaaatat
     6301 aagtattaat tcagcattta tttatgtaat atatattgac gatatgaggg tattaacata
     6361 tattaaatat agatatatat ctatataagg gggtggttaa gtgaatctgg attataatga
     6421 aaacgcaaaa attatcaaag cgctttcaga tccaagcagg ctaaaaataa tcgacatttt
     6481 gtcctgcggg gaaatgtgcg cctgtgatat attgaaacat tttgacttta ctcagccaac
     6541 actttcacat catatgaagg tattgatgga gtgcggcatt gtggaatgca gaaaagaagg
     6601 gctttggaac tattattcat tgaacggtac aaactgcaat aagcttatac tgtttcttat
     6661 gagtattatt acaaatacag atgattgtat ctgtaaagaa aaacccaaat gcggatgtga
     6721 atgtgaggga aaataaatga acaataaaca aaagtcaata gaaagggata atgaagccct
     6781 tggttttttt gccaagtatc tgacagtgtg ggttgcaata tgtatagctg cgggtgtcat
     6841 tcttgggcag gtactgccga tagtgccgga aaccctgagc aaatttgaat acgcaaatgt
     6901 atcaataccg gtagcgatac ttatttggct gatgatatac ccgatgatgc ttaagattga
     6961 tttttcaagt atagttaacg caacaaaaaa gccaaaaggc ctgatagtta cctgtggtac
     7021 aaattggctg ataaagccgt ttaccatgta tgcaatagct gcacttttct tccatgtagt
     7081 tttcaaagcc ctgattcctg ctgaattggc caaggaatac ctggccggag cagtactttt
     7141 gggtgcggcc ccctgtacgg caatggtatt cgtatggagc catcttacaa agggagatcc
     7201 tgcttatacc ctggtgcagg tagcagtaaa tgatttgata ctgcttgccg cctttactcc
     7261 tatagtggct tttctgcttg gtgtcagtga tgttgctgtt ccatatgata cattattcct
     7321 gtcggttata ttgtttgtgg taataccttt ggcagggggc tatttctcga ggaagtatat
     7381 aattaagaac aaaggcttgg actatttcaa caatgttttt ttaaaaatgt ttgacaatac
     7441 cacagtaata ggtttactgc ttacattaat tatcattttc tcattccagg gagaaatcat
     7501 attgaataat ccgctgcata tagcacttat agccgtaccg ttgacaatac agacctttct
     7561 tatattcttc ctggcttacg gctgggcaaa ggcatggaag cttaagcatg atattgcagc
     7621 cccggccggg atgatagggg caagcaattt ctttgagctt gcagttgcag tggctatttc
     7681 gttgttcggg cttgaatcag gtgcggcatt ggctactgtt gtaggggtac tggtggaggt
     7741 tccggttatg cttgcattgg taaagattgc aaacagtaca aggcattggt ttgctgaatg
     7801 atatttccaa tgtattgcag gcatcctgta ttgaggttgt tgtcaacaaa atcaatcata
     7861 attttaaaaa aagttcatgc tgcggaagtg gagagaataa atgctgctaa agttataaat
     7921 cctttgcatt actgggattt gtattcttat tgggggaggt gagatcaatg agctatatta
     7981 ctcagatatt cagttggtta aatgctcagc tgcttaaaat gacgtggctg tcggagctgg
     8041 ttgaagcttt ggtggagaag gtatttggcc tatccgtcga aacaaggctt ggaggaagca
     8101 ttcacttctt tatatatgat gtaattaaga tatttatact tctgtctgta ctcatatttg
     8161 caatatccta tatacagagc tattttccgc ctgagaagac caagaaaata cttggtggct
     8221 tcaaaggtat caaggggaac attttaggag cattgttagg tactataaca cccttctgaa
     8281 gttgctcaag tataccgata tttatcggtt ttacctcggc cgggctgccg ttgggtgtaa
     8341 cattctcgtt tttaatatca tcaccattgg ttgatttagc ttcaatgctg atactgatgt
     8401 cattctttgg agctaagata gcaattgctt atgttgttgt cggactgata cttgcggttg
     8461 caggtggtac ggtcattgac aagctgggac ttgaaaaata cgttgagagt tatgtaagag
     8521 atatagagaa tgtcgacttg gagatgtcgg aaatgacccg ccatgaaaga atttcttatt
     8581 caaagcagca ggtaaaggat attattaaaa aagtctggct gtatatattg attggtgttg
     8641 gcataggagc cgcaatccac aattggattc ctcaaagcat agtggaaaat gtcataggag
     8701 aaaacaatcc ttttgcggtt ttattggcaa ctgctatagg agttccgatg tatgctgaca
     8761 tattcggcac tattcctatt gcagaggcac tgtttaccaa aggtgcagga gtaggtaccg
     8821 ttctttcctt catgatggct gttactgcat tgtctttgcc ttcgatgata ttgctgagta
     8881 aggttgtaaa gccgaagctt ctggcaatat ttgctggaat agtgtctgtc ggtattgtga
     8941 taatagggta tttattcaat gcggtttctt ttattttaat ataatagaaa tattttaagg
     9001 aggtcaaaaa atggtaataa aagtattggg ttcgggctgc atgaactgca ggaagctaga
     9061 ggagaacaca aaagcggcag tgaaggaact gggtattgat gcaaaagtcg agaaagtgga
     9121 aaattacaaa gacatactgg cctatggagt aatgaaaaca cctgcacttg ttgtaaatga
     9181 ggaagtgaag gttatgggca gagttgcaga caccgaagag ataaaaaaga ttctgcgata
     9241 gcaaaatcag cgccgaactt ttatattatt cataacttct tcatattttc ttgctaatat
     9301 gataatgtaa aatgaaacag catatgttga aatttgaaca ttataagaat tacgggaaat
     9361 gccgtatctt gatacaaccg gagatagtac ggggaggtta agttatgaaa aatctgaaaa
     9421 aaataattga tgatttcctg aagaggctgg cagcccaaaa caaggagcag ttcggcagcg
     9481 aaaagctgga ctgctgcaac ttggacaggc ccaaaaatga tagcagagaa taggttcggc
     9541 ggtgagagat ttgaaggtat ccttaaagac taaactgaca ttgtcccatg ttgcaacagc
     9601 tttgatatgc gttgcattga taagcttgtt ttccaatatc ctgctggaaa ggcactttca
     9661 ggattacatc aggtcaaatc aggaaaaaaa gaataaagaa ttggtctcgc tcttttccag
     9721 caattatcaa agcaatgggc agtggaatgc ggaaagcgta caggatatag ggatatatgc
     9781 ccttgagcag ggaatgattg ttgaagtaag ggatcaatcg ggcaatataa tatggaatgc
     9841 catgtcctat aataacggtt ggtgtaacca aatgcttgat catatggcaa ataatatgta
     9901 cagccgttac cccaattggg agggtaagct ggtgcaggtt aactatcccg ttttctataa
     9961 aggagaaaaa gtcggcagta tagatatcgg gtattatggc cctttctatt ttaatgacaa
    10021 tgatatggca tttataagca cacttaatcg tatatttatt agtgtaggtg tcttttcgct
    10081 gcttctggcc tttttattcg gctcaattat gtccaggagg attacaatgc ctataacccg
    10141 ggttatcaac acggcaagga tgatagcaaa gggttatttc ggggacagaa gctttgaacg
    10201 aacaagtaca aaagaaatca gccagttgac agatactata aatgatttgg cggaaaccct
    10261 ggaaacccag gagaaactgc ggaagcgcct gacgggtgat gttgcccatg aacttagaac
    10321 acctgttgct acactgcaaa gccacctgga ggcaatgctt gacggaatat gggagccaag
    10381 ctccgacagg ctcaaaagct gccatgagga aacaatgagg ataaacagga tgatcggtga
    10441 cttggaaaag ctggcacgct atgaaagcga caatatgata ttgaatgagg aagagttttc
    10501 tttatgcgaa ttgataaaga taatggtaca gaactttgaa aaggagttta tacaaaggaa
    10561 tataaagatt caaatacagt gtgaaaacat tatgcttttt gctgacaggg ataaaatcag
    10621 tcaggttctt gtaaatctga tatcaaatgc attaaagtat accaacagca atggccaggt
    10681 tacaataacc gcctctgatt ccggcgataa tacgacaatt gcagttaggg acaccggtat
    10741 cggtatctca gaagaagatc ttccctatat atttgaacgt ttttataggt cggataagtc
    10801 cagaaacagg acgaccggcg gctccggcat aggattgact attgccaagg ctattgtgga
    10861 ggctcataaa ggcagcattt cagcatccgg caaattaaat gaaggtacgg tatttaccgt
    10921 tgctctgccg aaaaaaagat aatcgaaggg agatataagg agctagagct atatttgcag
    10981 tgtgcagatg ataggttcaa aggaattcaa taaagggatt ttgattcggt aaatatccgg
    11041 ataatatccg tctttcaatg aaaccataaa tatggtatat tctattatga tgagttttat
    11101 aggtaccatt agaaattcag agtaaaaaaa taagtgaggt agtaaagtga aaaacaaatc
    11161 atggattata ataattggga ttgtcgtctt gttggcggtt tatgttgtgg gggtacaaag
    11221 aagcctggtg aatttgagcg aggaagtgaa cgcatcctgg agcgacattg aagtacaata
    11281 tcaaagaaga gcggatttaa taccaaacat tgttgaaacg gtaaaagcac aggctataaa
    11341 agagcaggaa atttttacac agattgcgga agcgcgttca aagctgtcag gggcagcaaa
    11401 catacaggag aaaattgatg ccaatgcgga ggtttcgtct gtattgggaa gacttctggc
    11461 tatcgcggag aattatccgg agctgaagca gaatcaaggc ttccaggact tgagagtaca
    11521 gcttgaaggc acggaaaaca ggattgcagt tgccagaaaa tattataatg acaatgtaaa
    11581 ggaatataac aagaagataa aggtatttcc gacaaatata gcggcaggca tattgggtta
    11641 ttcggatgaa ttcccttata ttgaagcgga agccggtgcg gagcaggcac ctgatgtgaa
    11701 ttttgattaa ttcggaatgt gaaaggaact atactgcaac tctaaaacat agttttagag
    11761 ttgttcatct atttgaagac ttcgagatgg ggggcatgtg agtgaaacaa ttaaggttgt
    11821 ctgcagcatt tattgcagtt ttgcttctga ttgtcacgag ctttggagta ataatcgttc
    11881 agggagctga acctgaaata ccgaatccta caagggagtt ttatgtcaat gattttgccg
    11941 gtattctgga ttccggcacg gaacgggata ttctggcgct gggggaaaat acctacaaag
    12001 caacagatgg aggacaggtt gtatttgtca gtatcgatac attaaacgga aataccatag
    12061 aagagtatgc caatactctt ttcaacaaat ggaaaatagg cactgaggat aagggtattc
    12121 ttttcatact cagtatgaaa gagagagaat ccagaattga agtaggttat ggctacgaag
    12181 gcgtattgac agacttggaa agcagcaagc tgcttgtgaa gtttgccgga ctttatgaag
    12241 aaaaaggcat ggacgaagca gttaaaacaa tttattccga tatagccaat atcgtaagcg
    12301 ggaatgaaga tatggtccgg taccctgaaa tcggcgaaga aaggcagtat acaagtgatg
    12361 acttcagctt cttcagggag aaccctatta taactgtaat acttgtaatc attatattga
    12421 ttttgatagt cctggatttt atatttaccg gaggcagagt aaccttcttc ataattagaa
    12481 tggctgcttc gtccggcaga cgcggcggag gcggcggaag gaattccgga ggcggaggcc
    12541 gttccggagg cggaggcgct tccgg
//
LOCUS       DAOQUR010000065        12184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_5897_length_12184_cov_12.569956, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000065 DAOQUR010000000
VERSION     DAOQUR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5897_length_12184_cov_12.569956"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..916
                     /locus_tag="PKI50_10590"
     CDS             257..916
                     /locus_tag="PKI50_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04186.1"
                     /translation="MASWLTHLRVAEKIKQKIPEIDFPYFLIGSIAPDSGVSDEKQRN
                     YTPSKEITHYRYQKDDSISGMDDSVFFDSYLKPEKIMTRSDSTRSFLWGYYFHLITDK
                     LWLEEYFMPLKIQYENESSKGGKDFVDFIREEMVSLDFEYLEEKGTELINQLKDFNGN
                     ISFFSEFDSDYIYECKDRIADFYSGEPINLNKEYKYLTPQKIEDFVVKATEKCISILI
                     I"
     gene            938..1837
                     /locus_tag="PKI50_10595"
     CDS             938..1837
                     /locus_tag="PKI50_10595"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT04187.1"
                     /translation="MKYKGYILLAVTILMYSTLEVVTGAIKNLINPLQLTFLRFFIGG
                     VVMLPLIIKKREKVQGKDLLFFLALGILNIFISAGSLQYAITMGKASTAAILISSNPI
                     FVMLFSAIILKEKVTYNRIACILLGIAGITLIVYKGNMGGDTAASLILATFASTTFGL
                     YTVLGKLKSEGISSITMICYSSIFGSLMYVPVLLLNDIPLFYIPEGTIIRILYLGVFV
                     SGIAYVTYMEALKILTASKGAMVFFLKPVIASILAVIFLGESMSFKTVAGMIFVLAGV
                     FINFFTINLKSGKARDKFEETET"
     gene            1818..2423
                     /locus_tag="PKI50_10600"
     CDS             1818..2423
                     /locus_tag="PKI50_10600"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNT04188.1"
                     /translation="MKKQKLDNINQLHRKKLEEHFREEIHLVFGGGDIESKIVLVGEA
                     PGRTEVEQGKPFVGQAGKNLEEFINILGISREDLYITNVVKFRPVRYNQDTGRESNRT
                     PNKDEIRISTDFIEQELSIIKPELVVSLGNIALKGILKDDKATIGILHGSPIDVKFAG
                     TEFVLFPLYHPASIIYNRALKDVYLQDLHKLRNFMKKIKLI"
     gene            2432..3580
                     /locus_tag="PKI50_10605"
     CDS             2432..3580
                     /locus_tag="PKI50_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT04189.1"
                     /translation="MNTVSIVKCDDYEFNNVKRAVYEALDEIDNIKLKIIKGSRILIK
                     TNLLMKKNPEDAVTTHPAVVEAIVRYLQDIGCKVIIGDSPGGPFTEWNLKSVYKAAGM
                     LGVAERTGCELNFDTSVLEISNPDTKRLKSMQIIKIAKEVDFVVSAAKLKTHGMMTYT
                     GAVKNLFGVIPGLVKADYHLRLNNTDNFADHLVDICETVRPVFSIIDGIDGMEGDGPS
                     AGEKRHAGLIMASESPYALDTAASSIVGIEPMTVPTIRAASLRNLFSGDLKDIDIKGV
                     RLDEIRIEPFKLPKSHNANLIGGRVPKAFERMVLNTLRPKPVFNHEICISCGDCKRSC
                     PPKIIDMSSGKPVPDLSRCIRCFCCHELCPKKAVDIRRHWIYDKVLKK"
     gene            3627..4274
                     /locus_tag="PKI50_10610"
     CDS             3627..4274
                     /locus_tag="PKI50_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNT04190.1"
                     /translation="MLLQELREKVIEIALKAQKERLISMTMGNFSARDKETGYICITP
                     SGMEYDKLTAEDIVVMDAEGNILDGDRKPSIEHPLHCAVYRRRKDVFGVSHTHSVFAA
                     AWAACNENIPVVVAELAALVGGPVECAPYKPMGSSELAETAAVHLKDRHAILLANHGV
                     LAVGHDIEAAFANSVVVEEGAKIAYYARQIGVMKTIPEDECRSLRKSTLEKYGQK"
     gene            complement(4275..4916)
                     /gene="pgmB"
                     /locus_tag="PKI50_10615"
     CDS             complement(4275..4916)
                     /gene="pgmB"
                     /locus_tag="PKI50_10615"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HNT04191.1"
                     /translation="MSTIKGCIFDLDGVIVNTARYHYFAWKKLANELDFDFSEKDNES
                     LKGVSRMESLEILLKVGGLSFDDETKLALAARKNQWYVDYINSMEKDEVLPGVKGFLE
                     RARAAGLKIALGSASKNAPTILNRLKLADYFDVVIDGTKVSRAKPDPEIFLLAAQELG
                     LPPADCVVFEDAKAGVEAAKKAGMKCIGVGICSELADADAVIPGFENIDLSIL"
     gene            complement(5000..6220)
                     /locus_tag="PKI50_10620"
     CDS             complement(5000..6220)
                     /locus_tag="PKI50_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT04192.1"
                     /translation="MGDLKSIKGKLRTFHHATLMPINLLKGSKSVYCMPESINYISSI
                     IKDAYTIEDMPSLSPSGYSDNIKSYYFKNDYDECFIILHIKENYQLVAGPVLNEKIHE
                     GIINNIIRLNKLPIKIKSKLINYFENLLIVNTNRCYYCGKLLEYLFGTAVTDDLVGDT
                     SRERIFISDDYFTEMIKNREKLFHHPPYFLEQEMLSKIKSGNLNDTLAVLKEINSLKR
                     TRLSKDVLRSVKNSLICSVTLFTRAAIEGGVTPEAAFTLSDSIILAIEEINNMPELMD
                     YEYTAVKQYVQIVQDLSRIKYSNIIQQAISYIYKNLTNKLTLHKISDAIYVHPNYLSS
                     LFKREVGMSLSDYIMKTRVDESKYYIRYTNTKISDIAAFYQFCNQSYYTQVFRKFSGC
                     TPNEYRIKYNQQNE"
     gene            6343..7539
                     /locus_tag="PKI50_10625"
     CDS             6343..7539
                     /locus_tag="PKI50_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT04193.1"
                     /translation="MKKRVSLLLAIVMVFMAFCGFGMSVNADTRTVVKFAAQDDSTPA
                     TQILIDEFNKSQSKYKVEWVKMTNDSGQMHDQLMTSLSSGSSEYDVISMDVVWAGEFA
                     AAGFISPIDMNMYKDGLNKALYNAGSMAAGSYMGKQYTLPYFPDLGLLYFRSDIVSAT
                     DAAKLVTGNYTYDDLYNMAKKYKGRRGTTDGFVFQAKQYEGLTCNVTEFTGGLIDIKA
                     GLETMKKFVTSDFVPKDILNYTEAETHNSFINGKSVFARNWPYQYGMLADAPVKTYQV
                     GIAPLPKGSTVGGWLLGINKNSKNAEGAWEFIKYTSGWTGQMIMSRKGAYMPGYNYLL
                     TDERVENPLLSYEGLQNAMKATISRPISASYSKVSDTIQVNVHKYLSGGQDLNTTVTN
                     INNALR"
     gene            7626..8837
                     /locus_tag="PKI50_10630"
     CDS             7626..8837
                     /locus_tag="PKI50_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT04194.1"
                     /translation="MKRMGFKEALYILPAIIIIAIFSLWPILKVINYSVFDYQLNDQQ
                     KSKLSLDEKFNVDLFNENADYVIMFVDMNRFSFPEDTQGKVDEVIYNVNEFKKILSDK
                     SGFIELSGELKEKVRANYNKLIEDTDFFINNYDFPDRENLKLVMEDTKKCFVQSNFIG
                     LAGYSNALKDERVHDALWHTVVFTIISVALELVLGLGLAIIMNKAMKGQGIVRAVSLI
                     PWAIPTTVAALMWSYLYDGSNGIVANILQNLHIISDSRNLLLSGGGAMASTILADVWK
                     TTPYMALLILAGFQTIPGTVYESAKVDGSGVFNTFFKITLPLLKPSILVALLMRTLDA
                     FRVFDLIYVLTGGGPGGSTETMSIYAYKTMFQQTNFGYGSVIVLLMVVCVAIIATFYI
                     KILGANAMERD"
     gene            8849..9679
                     /locus_tag="PKI50_10635"
     CDS             8849..9679
                     /locus_tag="PKI50_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003347989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT04195.1"
                     /translation="MDKKSNKFKAMLFIFILLYLIVIIFPFIWVFLTSFKPTQEIFGE
                     GAFRLISESPTMENYANVLENGILQSMWNSFVVAGITSIYVVMVATLAAYAITRFKFR
                     GKNLLLGIILAVSMFPQMIIVGPIYNLFMNLNLTNSYFVVLPYSTITLPVAVFILVTH
                     FNKIPIALEEAATIDGATSMQILSKIVFRLAAPGIFTTAIIVFIAAWNEFLLTVTLNT
                     NKAYHTVPVAISFLRTQFTILWGDVTAATVIVTIPTLIIVLLFQKQIVSGLTSGAVKE
                     "
     gene            9714..12047
                     /locus_tag="PKI50_10640"
     CDS             9714..12047
                     /locus_tag="PKI50_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HNT04196.1"
                     /translation="MRLKAVYAKPYYRLDEWEIIEDSFDPDRNLHSETIFSIGNGFIG
                     IRGTFEEGLEDDSKPGAEGTFLNGVYEEGIIRYGEMAHGFPEKSQTMINAANGKKIIL
                     QLDDEIFNMQAGNILYYRRFINMREGILKRNLIWQSTMGKQIRLETERLVSFQRRNLA
                     VVRYKVTPLNFDGTIKIISEIDGDTKDALGEKDPRIGSSLEAGSFYTIDIVADSDTAA
                     LFQRTKRTGITVACVAKGAVRTECSYTSDKYICGDRVGFEYTVAGQKGKAIEYTKFIA
                     YEASIESCDSLMSHTLKVLESGMGKGYNELAREQREFLSDYWYRSDIEIKGDVALQQA
                     IRFNLFHLLQSAGRDGKTNICAKGLTGEGYEGHYFWDTEMYIIPAFLYSSPDISRKLL
                     EYRYGTLDKARERAREMSHKKGALFPWRTINGEECSGYFPAGTAQYHINAAIAHAIKK
                     YIEATQDDGFLLSYGAEILFETARLWADLGYFNAGKDGKFCINCVTGPDEYTAIVNNN
                     FYTNIMAKDNLEYAYNTAVWMREYHRPEYDRLAAEIGLGPEETELWKLAAGNMYLPYS
                     EKHGIHMQDDTFLDKKVWDFENIPRENYPLLLHYHPLVIYRHQVCKQADVVLALFLLG
                     HKFSLEQKRRDYEYYEKVTTHDSSLSACIFSIVANDIGYHEEAYDYFIKTARMDLDDY
                     QGNTHHGVHTANMAGSWMCIVNGFAGMRVYDDILSFKPYIHPDWESYSFKIAYRGRLI
                     KLTIRSGETSYELLGGNELEIAHRNKKYRLKDRLTLK"
BASE COUNT         3724 a         2176 c         2836 g         3448 t
ORIGIN      
        1 gtgtcgatgg tactcggacc gcagggtccc ggaagagtag atcgccgcca ggtaactaag
       61 aacgatagaa gaaattccat cgttcttttt tatacacttg ccttgtatgg gaaatgtatt
      121 cttcctgtta aagaatattt ctaaccggaa tacctttctg tgggtctgac accttttttt
      181 ctttaaaact aagtttttat atgatagaat caaaatatac gttatatatg cttcacagaa
      241 gaaagggtgc agacatatgg cttcgtggct cacacatttg agggtggcag aaaaaataaa
      301 gcaaaaaatt ccggaaatcg attttcccta ttttttaatc gggagcattg caccggattc
      361 cggggtgtct gatgagaaac agaggaacta cactccttca aaggaaataa cacattacag
      421 atatcaaaag gatgactcca tttcaggcat ggatgattct gttttttttg acagctattt
      481 aaaaccggaa aaaatcatga cgagatctga tagcacaaga tcttttctat ggggttatta
      541 ttttcatctg ataacggata agctttggct cgaagagtat ttcatgcctt taaagataca
      601 atatgaaaat gaatcaagca aaggtggcaa agactttgtg gattttatac gggaagaaat
      661 ggtttccctg gattttgaat atttagagga gaaaggaacg gaacttataa atcaacttaa
      721 ggactttaat gggaatatca gtttttttag tgaatttgat tctgattata tatatgaatg
      781 taaagaccgc atagcagatt tttatagcgg ggaacccata aatttgaata aggaatataa
      841 gtatctgact ccgcagaaaa tagaagattt tgttgtaaaa gctactgaaa agtgtataag
      901 catattgata atttaataag gatcacagga gagaataatg aaatataagg gttatatatt
      961 attggcagta acaatattaa tgtacagcac attagaggta gttaccggcg caataaaaaa
     1021 tctgataaat ccgctgcagc tgacgttttt gagatttttt atcgggggag tagtgatgct
     1081 gcccctgata atcaaaaaaa gagaaaaggt gcaaggtaag gatttattat tttttttagc
     1141 cttggggata ctaaatatat tcataagtgc gggcagcctt caatatgcaa taaccatggg
     1201 aaaggcttca acagcggcta tattgataag ttcaaaccca atatttgtta tgttgttttc
     1261 tgcaattatt ttaaaggaaa aggtgactta taacaggata gcctgtatat tgcttgggat
     1321 tgcaggtatt actctgattg tttataaggg aaatatgggg ggggatactg ccgcgagcct
     1381 tatccttgcg acatttgcat ctactacctt cggactttat actgtacttg ggaaactgaa
     1441 gtctgaagga ataagcagta ttacgatgat ttgctattct tccatttttg gcagcctgat
     1501 gtatgtgccg gtattgctat tgaatgacat tccgctgttt tatattccgg agggaactat
     1561 tatcagaata ctttatcttg gggtgtttgt ttcggggata gcctatgtta catatatgga
     1621 agcactgaaa atcctgacag caagtaaggg ggccatggta ttttttttaa agccggtaat
     1681 tgcctctata cttgcagtga tatttttggg cgaaagtatg agttttaaaa cagtggcagg
     1741 tatgatattt gtacttgccg gtgtttttat aaattttttc acaattaatt taaagtccgg
     1801 gaaagcaagg gataaatttg aagaaacaga aacttgataa tatcaatcaa ttgcacagaa
     1861 agaaactgga ggaacatttc agagaagaga tacatctggt tttcggcggc ggagatatag
     1921 aatctaaaat agttctggtg ggtgaggcgc ccggaagaac tgaagtggaa cagggaaagc
     1981 cttttgtagg gcaggcagga aaaaacctgg aggagttcat aaacatactg ggtatttccc
     2041 gggaggattt atatattact aatgttgtta agttcagacc tgtaagatac aatcaggata
     2101 caggaaggga atccaacaga acgccgaata aggatgaaat aaggatatct acagacttta
     2161 tagaacagga gctgtcaata ataaagcctg aacttgtagt cagtttggga aatattgcac
     2221 ttaagggtat attgaaggat gacaaagcaa caataggcat attgcacggt tcacccatcg
     2281 atgttaagtt tgccggcacg gaatttgtgc tgtttccttt atatcatccg gcaagcatta
     2341 tatataacag ggcgctaaag gatgtgtatt tgcaggatct gcacaagctc aggaatttta
     2401 tgaaaaaaat aaaactgata taggagcagc tatgaatact gtaagcattg tcaaatgtga
     2461 tgactatgaa tttaataatg taaaaagagc cgtttatgaa gccctggacg agattgataa
     2521 tataaagctc aaaataataa aaggctccag gatccttata aaaacaaacc tattaatgaa
     2581 aaaaaatcca gaggatgcgg ttacgacaca tccggcggta gttgaggcga tagtgagata
     2641 ccttcaggat atcggctgca aggtaataat cggcgacagc cccggagggc cttttaccga
     2701 atggaatcta aaatctgtct acaaagccgc cggtatgctt ggggtggcgg agaggacagg
     2761 ctgtgagctg aattttgata catccgtgct ggagatatca aatcccgata ccaaaaggct
     2821 gaaaagtatg cagattataa aaatagccaa agaagttgat ttcgtggtgt cagccgctaa
     2881 gctgaagact cacgggatga tgacatacac cggagcggta aagaatctct ttggtgttat
     2941 acccgggctt gtaaaagcgg attaccattt gcggctcaac aatactgata attttgccga
     3001 ccatctggtg gatatttgtg aaactgtaag gccggttttt tcaatcattg acggaattga
     3061 cgggatggaa ggggacgggc cttccgccgg tgaaaaaagg catgcagggc taattatggc
     3121 ctcagaaagc ccctatgctc ttgatactgc ggcttcaagc atagtaggga tagagcctat
     3181 gacagtgcct actatcaggg cggcttcatt gcggaacttg ttcagcggag atttgaagga
     3241 tattgatata aaaggagtgc ggcttgacga aataagaatt gaacccttta agcttccgaa
     3301 gtcgcataat gccaatttaa taggaggaag ggtgccaaag gcttttgaaa gaatggtctt
     3361 aaacactctc aggcccaaac cggtatttaa tcatgaaata tgcatatcct gcggagactg
     3421 taagagaagc tgtcctccga aaattataga catgagcagc ggcaagcctg tgccggattt
     3481 aagcaggtgc ataagatgtt tctgctgtca cgagctttgc ccaaagaagg ctgtggatat
     3541 aaggagacat tggatttatg ataaagtgct gaagaagtaa aagcagtgaa agaatattca
     3601 ctgtgtattg atgggaggtt tatacaatgc tgctgcagga gcttcgggag aaggtaatag
     3661 aaattgcttt gaaagcgcag aaggaaagat tgatttccat gacaatgggt aatttcagtg
     3721 cgagggacaa ggagacagga tatatctgca ttacaccaag cggtatggag tatgacaaat
     3781 taacggctga agatatagtt gtaatggatg cggagggcaa tatactggat ggagacagga
     3841 agccttcaat agaacatcct ttgcactgtg ctgtgtacag gaggcggaag gatgtattcg
     3901 gggtatccca tactcattcg gtttttgctg cagcctgggc agcttgcaat gagaatatac
     3961 cggtagttgt tgctgaattg gcagcacttg taggaggacc ggtagagtgt gcgccgtata
     4021 agcccatggg ttcttctgag cttgccgaaa cagccgctgt ccaccttaag gacagacatg
     4081 ccattctttt ggcaaaccac ggcgttcttg cagtgggaca tgatatagaa gcggcttttg
     4141 caaactcagt tgtagttgaa gagggagcaa agatagccta ctatgcaaga cagataggtg
     4201 taatgaagac tattccggaa gatgaatgca gatccttaag gaaaagcacc ttggagaaat
     4261 atggtcaaaa atagttacag tatgcttaag tctatgtttt caaagccggg aattaccgca
     4321 tcagcatcag caagctcaga acatatacct acccctatac acttcatgcc cgcctttttt
     4381 gcggcctcca cccctgcttt ggcatcttcg aaaaccacac agtctgccgg aggtaaccca
     4441 agctcttgtg ctgcaagcag aaatatttcc ggatcgggtt ttgcccttga tacctttgtt
     4501 ccgtcaatga cgacatcgaa ataatctgca agcttcagcc tgtttaatat agtcggcgcg
     4561 tttttgcttg cagagcccaa agcaattttc agaccggcag ctcttgccct ttctaaaaat
     4621 ccctttaccc cggggagtac ctcatccttt tccatggagt ttatataatc gacataccac
     4681 tgatttttcc tggcagccag ggcaagcttg gtttcatcat caaaggacag acctccaacc
     4741 ttgaggagaa tttcaaggga ttccatcctg cttacgccct tgagactttc gttatctttc
     4801 tccgagaagt caaagtccag ctcattggca agctttttcc atgcaaaata atgatacctt
     4861 gcggtattga caattacccc gtccaaatca aatatgcagc cttttattgt actcatgcag
     4921 cctcctgaag taatatatta gaataattag taagattata taaaagttac cttcaggttg
     4981 caattgtgaa tatttacaat tattcattct gttgattgta ctttattcta tactcattgg
     5041 gtgtgcagcc tgaaaatttt ctgaaaacct gtgtatagta gctttggttg cagaactgat
     5101 aaaacgcagc tatatcagat atcttggtat ttgtatatct tatatagtat tttgattcat
     5161 caacccttgt cttcataatg taatcagaaa ggctcattcc tacttccctt ttgaataagc
     5221 tggataagta attggggtgg acatatatgg catctgatat cttgtgaagc gtaagcttat
     5281 tggtaagatt cttataaata taggaaatgg cttgttgtat tatatttgaa tatttgatcc
     5341 tggataagtc ctgaacgatc tgcacatatt gctttaccgc agtatactca taatccatca
     5401 gctcaggcat attgtttatt tcttcaatgg caagtattat agaatcgcta agggtaaagg
     5461 cggcttcagg ggttactcct ccttcaatgg cagctctggt gaacaaagta accgagcaaa
     5521 tgagagaatt tttcaccgat ctcaggacat cctttgacaa ccttgttctt ttaagggagt
     5581 ttatctcctt tagtactgcc agagtatcgt tgagattgcc ggactttatt tttgaaagca
     5641 tctcctgttc caggaaatat ggagggtgat ggaaaagctt ctctctattc ttgatcattt
     5701 cggtgaagta gtcatcagag atgaaaatac gttcccggga ggtatctcca accaagtcat
     5761 cagttacagc agtgccgaac aggtattcca aaagctttcc gcagtagtag cacctgtttg
     5821 tgtttacaat aagcaagttt tcaaaataat ttataagctt gctcttaatt tttattggta
     5881 atttgttaag tcttattata ttattaatta taccttcatg aattttttcg ttgagaacag
     5941 ggcctgcaac taattggtaa ttttctttta tatgcaggat aataaagcat tcgtcataat
     6001 cattcttaaa gtaataggat ttgatattat cagaataacc ggagggagaa agactgggca
     6061 tatcttcaat tgtatatgca tcttttatta tgctgcttat ataattgatg ctctcaggca
     6121 tacaatatac gctcttactg cccttaagca ggtttatggg catcagagtt gcatggtgaa
     6181 aggttcggag cttccctttg atactcttaa gatcccccat gattaccctc ctttacacga
     6241 ttgttaatat taatatttta acataaatat taatatattg gtataattaa tgcccggctt
     6301 cctttagaat gaaattagta aattatacga ggagggtaat aaatgaagaa aagagtgtct
     6361 ttattattgg ctatcgtaat ggttttcatg gctttctgcg gctttggcat gagcgttaat
     6421 gccgatacca ggacagttgt caaattcgcg gcgcaggatg actcaacccc tgctactcag
     6481 atacttattg atgagttcaa caaatcccaa agcaaataca aagtggaatg ggtaaagatg
     6541 acaaatgatt cgggacagat gcatgatcag ttgatgactt ccttatcctc aggctccagt
     6601 gagtatgacg ttatatcaat ggacgttgtc tgggcgggtg aatttgcagc cgccggattt
     6661 ataagcccca ttgatatgaa tatgtataag gacggtttaa acaaggcttt gtacaatgca
     6721 ggctcaatgg cagccggaag ttatatggga aagcagtaca cactcccata cttcccagat
     6781 ctgggactgc tttatttccg ctcggacata gtaagcgcaa ctgatgcagc aaagcttgtg
     6841 acagggaact atacgtacga tgacctgtac aatatggcta agaaatataa aggaagacgc
     6901 ggaactacag acggttttgt cttccaggca aagcagtatg aagggcttac ctgcaatgta
     6961 actgagttta caggcgggct cattgatata aaagccggcc ttgagacaat gaaaaagttt
     7021 gttacctcag attttgttcc aaaggacata ttgaattaca ctgaagcaga aacccacaat
     7081 tcatttataa acgggaagtc tgtatttgcc aggaactggc cctaccagta cggtatgctg
     7141 gcagacgcac ctgtaaagac ttaccaggtt ggaatagctc ctcttcctaa aggaagcacg
     7201 gtaggcggat ggctgcttgg cataaacaaa aactcaaaaa atgctgaggg tgcatgggaa
     7261 ttcattaaat acacatcagg ctggacagga caaatgatta tgtccagaaa gggcgcatat
     7321 atgcccggat ataactatct gcttactgac gaaagagttg aaaatccgct tctttcatat
     7381 gaagggcttc agaatgcgat gaaggcaaca atatcaaggc cgatttcagc cagctattcc
     7441 aaggtttcgg atacaataca ggtaaatgtt cataagtatc tgtccggggg acaggatctg
     7501 aacactactg taacaaatat aaataatgcc ctgaggtaat attcggttta aagaacttag
     7561 aggaattggg ctccggttat actcgggccc aattcttttg aaattccttc tttgggggtg
     7621 gtcacatgaa aaggatgggg tttaaggaag cactctatat attaccggct atcattatta
     7681 tagcaatctt ctcactttgg cccatactta aggtaatcaa ttactccgtt tttgattacc
     7741 agttgaacga tcagcagaaa tccaagcttt ctctggatga aaagtttaat gtagaccttt
     7801 ttaacgaaaa tgcggattat gtgataatgt ttgttgatat gaacagattc agttttccgg
     7861 aagatactca gggaaaagta gatgaagtga tatataacgt caatgaattt aaaaaaatat
     7921 tgtctgataa gtcaggattt attgagctta gcggagaact taaagaaaaa gtcagggcta
     7981 attacaataa gcttatagag gatacggatt tttttatcaa caattatgat ttcccggata
     8041 gggaaaacct caagctggta atggaggata ccaaaaagtg ctttgtacag tcaaatttca
     8101 tagggcttgc cggatacagc aatgcactga aggatgagag agtgcacgat gcactttggc
     8161 ataccgtagt attcacaata atatctgtcg cccttgagct tgtacttggg ctcggactgg
     8221 caataatcat gaacaaagcc atgaaagggc agggtattgt acgtgccgtt tccctgatac
     8281 cctgggcaat accgaccacc gtcgccgccc ttatgtggag ctatctgtat gacggaagca
     8341 acggtatagt ggcgaatatt ctgcaaaacc ttcatattat aagcgattcg aggaatttgc
     8401 tgctgtccgg cggaggagcc atggcatcta caatacttgc ggacgtatgg aagaccacac
     8461 cctatatggc tctgctcata cttgcgggct ttcagactat accgggaacg gtgtatgaaa
     8521 gtgcaaaggt tgacggatca ggggtattca ataccttctt taaaataaca cttcccctgc
     8581 tgaagccttc tatacttgtg gcccttctta tgagaaccct ggatgctttc agggtatttg
     8641 accttatcta tgtgcttacc ggaggcggcc ccggaggttc aaccgagacc atgtccatat
     8701 atgcatacaa gaccatgttc cagcagacca attttggtta cggttcagtt atagtcctgc
     8761 ttatggttgt ttgcgttgca ataattgcga ctttctatat taagatactc ggtgcaaatg
     8821 ccatggaaag ggattagggg gtgacgtaat ggataagaaa tcaaataaat tcaaggctat
     8881 gctgtttata ttcatattgt tgtatttgat tgtcataatt tttccgttta tctgggtttt
     8941 cctgacctct ttcaagccta cgcaggagat attcggtgaa ggtgctttca ggctaatatc
     9001 agaaagcccg actatggaga actatgccaa tgttttggaa aacgggatac tgcagtccat
     9061 gtggaacagt tttgtggttg ccggaataac ttcaatttat gtggttatgg tagcgaccct
     9121 ggctgcatat gcaattacaa ggttcaagtt cagggggaag aacctgcttc ttggaattat
     9181 tcttgctgtt tcaatgtttc cgcagatgat aatagtaggg ccaatttata acttattcat
     9241 gaatttgaat ctgacaaaca gttattttgt tgtactgccg tactccacca taactttgcc
     9301 cgtcgcggtg tttattctgg ttacgcattt caacaagatt cctattgcat tggaggaagc
     9361 tgctactatt gatggggcta catccatgca gatattatca aagatagtgt tcaggctggc
     9421 ggctcccggg atattcacta ctgcaataat tgtgtttatt gcggcatgga atgaattcct
     9481 gcttaccgta acattaaata caaataaagc ctatcatact gtcccggtgg caatttcctt
     9541 cttaagaacg cagttcacca tactatgggg tgatgtcact gcggctaccg ttattgttac
     9601 tatacccaca ttaattattg tactgttatt ccagaagcag atagtatccg gcctgacctc
     9661 cggagcagtc aaggaataga ggaacgctgc aaaatcagaa aggggcatac aacatgagat
     9721 taaaggctgt atacgcaaag ccatactaca ggcttgatga atgggagatc attgaagaca
     9781 gttttgaccc tgacaggaat ttacatagtg aaactatttt ttccattggg aatggattca
     9841 taggaataag ggggactttt gaagaggggc tggaggatga ttcaaagccg ggggcggaag
     9901 gaaccttttt gaacggcgta tatgaagagg gaatcataag atatggtgaa atggcacacg
     9961 gttttcccga gaaaagccag actatgatta atgccgctaa tggcaagaaa atcatactcc
    10021 agctggacga tgaaatattt aacatgcagg ctggaaatat cctttattat aggcgtttca
    10081 tcaatatgag ggaaggaata ttaaagcgca atctcatatg gcagtcaact atggggaagc
    10141 aaatcaggct ggagacagag agattggtaa gctttcaaag aaggaacctg gcagttgtaa
    10201 ggtataaagt aactcctctt aattttgacg gtacgataaa aattatctcg gagattgacg
    10261 gagatacgaa agacgctctt ggtgaaaagg atccgaggat aggttcgagc cttgaggcag
    10321 ggtcttttta tactattgat attgtggcgg attcagatac agcggccctt ttccaaagga
    10381 caaagaggac agggataacc gttgcatgcg ttgcaaaagg tgccgtaaga actgagtgct
    10441 catatacatc tgataaatac atatgcggag acagggttgg atttgaatac actgtagccg
    10501 gtcaaaaggg aaaagctatt gaatatacca agtttatagc atatgaagct tccatagaaa
    10561 gctgtgacag cctgatgagc catacactta aggtgctgga aagtggaatg gggaagggct
    10621 ataatgaatt ggcaagagag cagcgtgagt ttctgtcgga ttactggtat aggtctgata
    10681 ttgaaataaa aggcgacgta gccttgcagc aggcaataag attcaacctt ttccatcttc
    10741 tccaatcggc aggcagagac ggaaagacaa atatctgtgc caagggcctt acgggagaag
    10801 gctatgaagg acactatttc tgggatacag agatgtatat catacccgcc tttttgtaca
    10861 gcagtcctga tatcagcagg aagctgcttg aatacagata tggcactctg gacaaggcaa
    10921 gggaaagggc aagggaaatg tctcacaaga agggagcgct atttccatgg agaacaataa
    10981 acggggagga gtgctcagga tattttcctg cagggactgc gcagtaccat attaacgctg
    11041 caattgccca tgcaataaag aagtacattg aagcaacgca ggatgacggg ttcctgctta
    11101 gctatggggc tgaaatactc tttgaaacag ccagattgtg ggcagatttg ggttatttta
    11161 acgccggtaa ggacggcaag ttctgcataa actgcgttac agggcctgat gaatatactg
    11221 caattgtcaa caacaatttt tacacgaata ttatggcaaa ggataatctt gaatatgctt
    11281 ataatacagc tgtatggatg agggaatacc acaggccgga gtatgacagg cttgcggcag
    11341 agatagggct tggaccggaa gaaacagagc tgtggaagct tgcggccggc aatatgtatc
    11401 tgccctatag tgagaagcat ggaatacata tgcaggatga tacttttctg gataaaaagg
    11461 tatgggattt tgaaaatatt ccaagggaga actatccttt actattacat tatcatcctc
    11521 tggtcatata caggcatcag gtgtgcaaac aggcggatgt ggtactggcg cttttcctgc
    11581 ttgggcataa gttcagcttg gagcagaaaa ggagggatta tgaatactac gaaaaggtca
    11641 ctactcatga ttcttccctg tcagcatgca tattcagcat agtggccaac gacataggct
    11701 atcatgaaga ggcctatgac tatttcataa aaacggcaag aatggatttg gatgattacc
    11761 agggcaacac ccatcatgga gtccatacgg ccaacatggc aggcagctgg atgtgcatag
    11821 tgaacgggtt tgccggaatg agggtatatg atgatatatt gagcttcaaa ccgtatattc
    11881 atccggactg ggagtcgtac agcttcaaga ttgcctacag gggaagactg ataaagctta
    11941 caataagaag cggcgagacc tcttatgagc tgcttggggg taatgaactg gagattgctc
    12001 acaggaataa aaaatacagg ctaaaggata gactaacgct gaaataggct atttgaataa
    12061 atagtctatt tttatgctca aaagcagttg tactttatca aaaaataata tatgacgtga
    12121 aaaagattta tcaacaaaaa atgtaaaatg tttggcacga gatatatggt atattataat
    12181 tacc
//
LOCUS       DAOQUR010000066        11269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_6497_length_11269_cov_9.577492, whole genome shotgun sequence.
ACCESSION   DAOQUR010000066 DAOQUR010000000
VERSION     DAOQUR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_11269_cov_9.577492"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..812
                     /locus_tag="PKI50_10645"
     CDS             111..812
                     /locus_tag="PKI50_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04197.1"
                     /translation="MGYSSNMTVLGSVFPDTVIKCSLTRDQTHNVGWGLYKYLKGHGN
                     EYLEFARAMATHTVNPRGLDFYGDEEYGDGYKGYCFQKAAAIEKEVIEACNIPAEFGL
                     WKAHNFIEMGIEMNIAESERELMDVFHKGLTDYCLIKELSLYLDRYYGLKDKSVSDCF
                     CKFTEFMELKDLNSFTLARRYNTQMNYKHGIEIDISKCSNIIDKARGIVQEDFESFIS
                     YCAEKVKASLKEECT"
     gene            803..2449
                     /locus_tag="PKI50_10650"
     CDS             803..2449
                     /locus_tag="PKI50_10650"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HNT04198.1"
                     /translation="MYLKKEISVIGHKNPDTDSICSSIAYANLKNIITSSNVYFPKRI
                     GELNSETLFVLNYFKAGEPILIDNVRTQVKDINIRKVELVKPEISLKKAWEIMKSSGI
                     TTLAVSHNNRTLDGIIAVDDIAHSFMDIYDNDILSQARTPYRNMVEVLDGEILVGDIE
                     DSVYNGNVHIGAMNPDLMESYIHAKDVVIVGNRYEAQLCAIEMGAQCIVVTGGVPVAS
                     SIKKLAEAQNCAIVVSPHDTYTVARLINQSIPIRQFMKSNELVTFNINDYLDDVREVM
                     VKKRFRNFPVVDDKNNMIGMLSRASLINSPRKQVILVDHNERSQAVDGLEEAEILEII
                     DHHRIGDIETVGPVYFRNQPLGCTATIVRNMYMENNVEISKKMAGLLCSAILSDTLAF
                     KSPTCTETDREAAESLAEIAGLDMDEFSMIMFRASSNLMDKSAEEIFYQDFKQFKTGE
                     LELGVGQVYSMDSSELLEIKDRMLEYMDKVYRDKKLDMVLFMLTDISDERTELLFIGS
                     NTELISKAFDGKHDKNSIILPGVVSRKKQVIPPLLSSVNQ"
     gene            2517..3293
                     /locus_tag="PKI50_10655"
     CDS             2517..3293
                     /locus_tag="PKI50_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNT04199.1"
                     /translation="MEFEITVLNVVFLCLAGFAAAAVDAIAGGGGLISLPAIMAAGVP
                     PHFALGTNKFSSSFGAVTSAYTFIRSGKVHIPLMKFTVPCTLAGSVIGVFTALRIDQK
                     FLRLIILVLIFATAIYTIAKKDFGSEDKFKELTGRNIIKGCLFAFSLGFYDGFFGPGT
                     GSFLIFLFIKVYGFDFTIAAGNSKVLNFVSNFTSLVMFAINGKIYYMLGIPIGLAMIA
                     GARMGSKIAIKNGAKVIKPIFVTIALALTLKLLFDAVRLL"
     gene            3328..4275
                     /locus_tag="PKI50_10660"
     CDS             3328..4275
                     /locus_tag="PKI50_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNT04200.1"
                     /translation="MVRGIDKYILGISEIFDAPTEDKGLRISLTEHRDVRNIIKKRER
                     DSHKGTYGRVGILSGSKGMAGAAVLNCNAALRSGSGLVKACVTEAIYCIVESMALEAI
                     TYAFDEQNIDYDELDRELIQFSDVIATGSGCANMKRYGELLRFLTENSQKPLVIDAEG
                     INLLTPDILNNHRQIMILTPHYGEMARLINKDPDYMRENIIEKAAEFACKYNVYLVLK
                     GARTVIACPQGNSYVNTTGNPGMATAGSGDVLTGIIASLIGQKIEIPDALRAAVYIHG
                     LAGDIGSSCAGEYSLIAGDIIRYLPEAFKAVMGTGGCVT"
     gene            4431..5381
                     /locus_tag="PKI50_10665"
     CDS             4431..5381
                     /locus_tag="PKI50_10665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNT04201.1"
                     /translation="MKEVTFDDYRQHYRYKYVVENIKDVIWEMDENLIFTYISPSCKE
                     MSGYESSEFIGKRMTDFLVPESRNYVLDQWKKNAHSRKSCNSRGIIMYDLQFICKDKG
                     LIWVEVSVKPVFEDDRFLGYIGVTRDVTEKKVHENELKKYYNELETANRELEKLATFD
                     MLTGAYNRRRFEEYVKSAVDMKEMHGSPFSIIIFDIDCFKLINDIYGHSWGDRILQVV
                     TEIVKASIRETDKLFRWGGDEFIILMPGKASKDAYKVAERVRKSIEKNNFGIQHDRIT
                     ISTGVGEYITDESIDQFVSRVDDALLTAKSIGRNKVELCI"
     gene            complement(5386..6171)
                     /locus_tag="PKI50_10670"
     CDS             complement(5386..6171)
                     /locus_tag="PKI50_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT04202.1"
                     /translation="MDKNNEKNTKTIGSVIKAIEILEELAESEKGLGLTEISNSLNYG
                     VSATYHLLNTLKLHNIIEQNKKNKKYRIGVGLFRISSKAKSQYSLVNLTMPFLDKLRA
                     IVDETSNLLVLDGYEVLYIAQSESTKLLKMFTQLGAKAPLYCTGGGKAILAYKPLSFQ
                     EHALSKINFIKYTENTIVTADSLRKEFEIIKKQGYSIDNEEREEGITCAAAPIFDCYG
                     EVIAAVSISGPSYRIKEKDLKSIISSVVLTAKEISANLGYMEK"
     gene            6470..7747
                     /locus_tag="PKI50_10675"
     CDS             6470..7747
                     /locus_tag="PKI50_10675"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNT04203.1"
                     /translation="MLSKKVMENYILILKSELIEALGCTEPIAIAYAAAKARSVLGKA
                     PERIEVGCSGNIVKNVKGVVVPNTGGLIGIDAAAVAGVVGGDPDRGLQVLESVRRKQY
                     CTVKRLTEEGYCKVYHLKGVDNLYIEAKVYAGNESAEVYIVDAHTNIVKIVKDGKVLF
                     ENNNGGRKDSKLSGDKTLLNIKDILEFAETVDLDILHELLEKQVTVNTAIAEEGIRND
                     YGASVGKTLLENYGNDVTVRARAMAAAGSDARMSGCSLPVVINSGSGNQGITVSVPVI
                     EYARELKTSEEKLFRALIISNLVSIHQKAQIGNLSAYCGAVSAAAGSGAGIAYLNGGG
                     YKEISSTIINTIANIGGMVCDGAKPSCAAKISSAVGAAILGYQLSARGKVFRSGEGLV
                     KGGVEETIKSFGRVGKKGMEGTDIEIINIMLDK"
     gene            complement(7755..9542)
                     /locus_tag="PKI50_10680"
     CDS             complement(7755..9542)
                     /locus_tag="PKI50_10680"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT04204.1"
                     /translation="MSYLKLIDDKIQSLAESLSSILNVETTVVDEHLLRIAGTGDFYH
                     RINESSPENSLFAKVLKTGKAELNLYDKKNPICQTCNNSDTCKELKSMILPIKVENDV
                     IGVVSFVSFNAAQDQLMVEKKEEYLEILKHFAETIEKEIISIKMVNKLNMGIAEINGI
                     INSINRSIIILNKEQRIIYINSKAIKSLNINFSADKIINKRIGSIIKGFKAEDTNNKE
                     VIGSWAIGGKLIKVIYKVNYIILDNKNVSTIINFDTLDEIISIAMTYNSDEETTFENI
                     IGRSQIMQDVIEKAKIAAKSDSTIFLHGTSGTGKELFARSIHNASYRKDGPFVAVNCA
                     ALPENLIESELFGYEKGSFTGASQKGKTGKFEQANNGTLFLDEIADLPLHLQAKLLRV
                     LQDKKIDRIGGSAQINVNVRIISATHKNLEEMVRNNEFREDLFYRLNVIPLHLPSLKD
                     REEDVLLCSEYIIKKLCLKMNKSPKRISKDVETMFMGYGWPGNVRELENVLEYAINFS
                     FDDEITAENLPEYFLANYAKKDNDEYINIESPEIDNLKSLGDITKEYEKKLIRKLISK
                     YGNTTETKRKIAEKLGMSITTLYRKLNDY"
     gene            9726..10874
                     /locus_tag="PKI50_10685"
     CDS             9726..10874
                     /locus_tag="PKI50_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNT04205.1"
                     /translation="MKTRKLLMIPGPTPVARSIQEQMGRETVAFGDPAFVKDFKELLE
                     DLKSLFNCSGEVFVVAGTGTMGMEMAIANTTKAGDNVLIVSHGFFGDRFIDLCSRKGL
                     NADVISSEWGETVPVEKIKEKLKEKKYAAMTVTHVDTSTGVCAPVAEIGKMMKEFPET
                     IYIVDGVCATAGEPEDVDAMGIDILFTGSQKAFGVAPGLTMLWASGKALERRKSLGSI
                     PEFYIDFEKWLPVMKDTAKYFATPAINMIWALKESVRIIKEEGIENRYARHRKTGTAM
                     QAALEALGFRILAKEGCRAATLSNPVYPEGVDDAKFRGLLAEEGIMVAGGLAAYAGRM
                     FRLGHMGNIDKHDMISVIAAVERALYRAGMPVELGKGVGVVQQHLIDS"
BASE COUNT         3416 a         1979 c         2678 g         3196 t
ORIGIN      
        1 aaatatatta atatagatgt agatgcagat atagatatag aagattaatg cgtgtgagaa
       61 aggaagaact aatgtttccg ataacacata tttggttttg caggagagta ttgggttaca
      121 gcagtaatat gactgttttg gggtctgttt ttcctgatac tgtgataaaa tgcagcctta
      181 cccgtgacca gacccataat gtgggatggg ggctgtataa ataccttaag gggcatggaa
      241 atgagtacct tgaatttgca agggctatgg ctacgcatac cgtaaatccg agaggccttg
      301 atttctacgg agatgaggaa tatggggacg gatacaaggg ctactgtttt cagaaggcgg
      361 cagcaataga gaaggaagta attgaagcct gtaatattcc cgcagagttc ggactatgga
      421 aggctcacaa ttttatagaa atgggtatcg aaatgaatat tgcggaatcc gaaagggaac
      481 tgatggatgt atttcataag ggcttgacgg actactgcct tattaaagaa ctttcattat
      541 accttgacag atactacggt ttgaaagaca agtccgtatc ggattgcttc tgcaaattta
      601 cagagtttat ggaattgaag gatctgaaca gctttaccct tgcaagaaga tataatacgc
      661 aaatgaatta taagcatgga atagaaattg acatatcaaa atgcagcaat atcatagata
      721 aagccagagg tatagtacag gaggattttg aaagctttat ctcatattgt gcggaaaagg
      781 tgaaagcatc gctgaaggag gaatgtactt gaaaaaggaa atttcagtaa ttgggcataa
      841 aaatcccgat accgattcca tatgctcatc catagcttat gccaatctta agaatattat
      901 cacatcaagc aatgtatatt ttccgaagag aataggagaa ttaaacagcg agacactttt
      961 tgtacttaat tatttcaaag ccggggaacc aattctgata gataatgtaa ggacacaggt
     1021 taaggatata aatatcagga aagtggagct tgtcaaaccg gagatatcct tgaaaaaggc
     1081 ttgggaaata atgaaaagca gcgggataac cacccttgca gtttcacata acaacaggac
     1141 actggacggg ataattgcag ttgacgatat tgcacattca tttatggaca tatatgacaa
     1201 tgatatatta tcccaggcca ggactccgta caggaatatg gttgaagtgc tggacgggga
     1261 aatactggtc ggggatatag aagactcggt atataacggt aatgtgcata tcggtgccat
     1321 gaatccggat ttgatggaga gctatataca tgcaaaagat gttgtaatcg tcgggaacag
     1381 atatgaggcc cagttatgcg ccatcgagat gggagcccaa tgtatcgtgg taacaggggg
     1441 agtacctgta gcaagttcta taaagaaact ggctgaggcc caaaactgtg ccatagtggt
     1501 ttcgccccat gacacctata cggtggcaag gcttataaat caaagcatac ctataaggca
     1561 gttcatgaaa tccaatgagc tggtcacctt taatattaat gattatttgg atgacgtaag
     1621 ggaagttatg gtcaagaaaa gattcagaaa cttcccggtg gtggatgata aaaacaatat
     1681 gatagggatg ctttcaaggg catcattgat taactcccca agaaaacagg tgatactggt
     1741 tgatcacaat gagcgttcac aagcagtgga tggccttgaa gaagcggaaa ttctggagat
     1801 aatcgatcac catagaatag gtgatattga aaccgtaggg ccggtatact tcagaaacca
     1861 gcccttaggc tgcactgcta cgattgtccg gaatatgtac atggagaaca atgtggaaat
     1921 aagcaaaaag atggcagggc ttctttgctc tgcgatatta tcggataccc tggcattcaa
     1981 atctcccacc tgtacggaga cggacaggga agcggcggag agcctggcgg aaattgccgg
     2041 attggatatg gatgagtttt caatgattat gttcagggcc agctcaaatc ttatggataa
     2101 aagtgccgag gagattttct accaggattt caagcagttc aaaacgggag agctggagtt
     2161 gggagtggga caggtatact ctatggacag cagcgagctt cttgagataa aggacaggat
     2221 gcttgaatat atggacaagg tctaccggga taagaaactt gatatggtgc tgttcatgct
     2281 taccgacatt tccgatgaaa gaaccgagct gctgtttatc ggcagcaata ccgaattgat
     2341 atccaaagca tttgacggga agcatgataa gaattcaata attctgcccg gagtagtatc
     2401 ccgcaagaag caagtaatac cgcccctttt aagcagtgta aatcaataac ggcagcagga
     2461 acagtgccct cactgccccg atgccgggct tataaatatg gattcaggag ttacaaatgg
     2521 aatttgaaat aactgtgtta aacgttgtat ttttgtgcct cgcaggcttt gcagcagctg
     2581 cggtggatgc tattgccgga ggcggcgggc ttatcagtct tcctgcaatc atggcagcag
     2641 gggtaccccc tcatttcgca ttaggaacca acaagttttc aagctccttt ggagctgtta
     2701 caagcgcata tacattcata cgctcaggaa aggtgcatat tcccctgatg aaattcactg
     2761 ttccgtgtac tctggcaggt tcagtgatag gagtatttac tgcgctgagg atcgatcaga
     2821 agtttctgcg attaataata ttggtgctta tttttgcgac tgcaatttat acaattgcca
     2881 aaaaggactt cggcagtgag gataagttca aagaacttac aggcagaaat attattaaag
     2941 gatgtttatt cgccttttcc cttggcttct atgacggctt tttcggaccc gggacgggtt
     3001 cttttctgat atttctgttc ataaaagtat acggctttga ttttacgata gcggccggta
     3061 acagcaaggt gctgaatttt gtaagtaatt tcacttctct tgtgatgttt gcgataaacg
     3121 gaaagatata ttatatgctt ggaataccca taggccttgc catgatagcc ggtgcaagga
     3181 tggggagcaa aatagccata aaaaacggtg caaaggtaat aaagccgata tttgtgacaa
     3241 tagcactggc gcttacactg aagcttttat ttgacgctgt aaggctgcta taaaaaagat
     3301 tgccgggaaa taaaggggct gtgaaaaatg gtcagaggca ttgacaaata tatattggga
     3361 atatcagaga tatttgatgc gccaacggag gacaaaggct tacggataag ccttactgag
     3421 catcgggatg taagaaacat aataaaaaag agagaaaggg attcacacaa gggaacctac
     3481 ggaagagtgg gaatactgtc aggctcaaag ggtatggcag gggcggcggt gctgaactgc
     3541 aatgctgccc tgcgcagcgg ttcaggcctt gtcaaggcat gtgtgactga agccatatac
     3601 tgtattgttg agtctatggc attggaagca ataacatatg cctttgatga acaaaacata
     3661 gattatgatg agctggaccg ggaattaata caattttcgg atgttattgc cacaggctcc
     3721 ggatgcgcga atatgaaaag gtacggagag ctattgaggt tcctgacgga gaattcacaa
     3781 aaaccactgg ttatagatgc agagggaata aacctgctga ctcccgatat attgaacaat
     3841 cacaggcaga ttatgatatt aactccccat tacggtgaaa tggcaagact gataaataag
     3901 gaccctgatt atatgagaga aaatataatt gaaaaagccg cggaatttgc atgcaaatat
     3961 aatgtttatc tggtgctgaa gggagcaagg actgttattg cctgccccca ggggaacagt
     4021 tatgtcaata ccacaggtaa tcccggtatg gcgacagcag gcagcggaga tgtcctgaca
     4081 gggataattg cttcgcttat aggccaaaaa attgaaatcc cggatgctct gagggctgcc
     4141 gtatacatac atggtttggc cggtgatata ggcagcagtt gtgcaggtga gtattccctc
     4201 attgccggtg atattatcag gtacctgccg gaagccttta aagctgtcat ggggacagga
     4261 ggatgtgtaa cttgaaccat gcgaaagcaa aaacagagcg taagaagaga tgcgaaatac
     4321 aaaaactttc aatacgaaat acagaaattt gtatcaaatt gtatcttttt atgtattaat
     4381 tcagtataat aattaagtat agcagatttt ttcgattgca ggtgaaatgt atgaaagaag
     4441 taacatttga tgattatagg cagcattatc gctacaaata tgtggttgaa aacattaagg
     4501 atgtaatctg ggaaatggat gagaacctga tttttactta cataagtccg tcgtgtaagg
     4561 aaatgtcggg atatgaaagc agtgaattta tcggaaaaag aatgacggat tttcttgttc
     4621 cggagtcaag gaattatgta ttggatcaat ggaagaaaaa cgcacatagc agaaagtcat
     4681 gtaattcaag agggatcatt atgtatgatc ttcaattcat ttgtaaggat aaagggttaa
     4741 tttgggttga ggtttctgtt aaaccggttt tcgaggatga tagattcctg ggttatatcg
     4801 gtgttacccg ggatgtcact gaaaagaaag tacatgagaa tgagttaaaa aaatactata
     4861 atgagctgga gactgcaaac agagagcttg agaagcttgc gacctttgac atgctgacag
     4921 gtgcatacaa caggcgaaga tttgaggaat acgtcaaatc tgccgttgat atgaaagaga
     4981 tgcacggcag ccctttttca attatcatat ttgatattga ctgcttcaag ctgataaacg
     5041 atatttacgg gcatagttgg ggggaccgga ttttgcaggt agtaacagag atagtcaaag
     5101 cttcaataag agaaacggac aagctgtttc gatggggcgg agatgaattc atcatactta
     5161 tgcccggaaa ggcctccaaa gatgcatata aggttgcgga aagagtcagg aaatctatag
     5221 agaaaaacaa ctttggaata caacatgata gaattacgat aagcacaggg gtaggggaat
     5281 acataaccga tgaaagcatt gatcagtttg tttcacgggt ggatgatgct ttattaacgg
     5341 caaaatccat cgggagaaac aaggttgaac tatgcatata gaggttcatt tttccatata
     5401 acccaaatta gctgatattt cttttgctgt caaaaccaca ctggatatta tactttttaa
     5461 atccttttct tttattctgt agcttgggcc tgatatgctt acggctgcaa taacctcgcc
     5521 ataacaatca aatatcggag cggctgcaca cgtaatccct tcttcccttt cctcattatc
     5581 tatggaatag ccttgttttt ttatgatttc aaattctttt cgcaaactat ctgctgtgac
     5641 aatggtattt tcagtatatt ttataaagtt gattttactc agggcatgct cttgaaagct
     5701 aaggggctta taagctaaaa tcgctttgcc gccgcccgta cagtacaggg gagcctttgc
     5761 cccaagctga gtaaacattt tcaggagctt ggtgctttcg gactgagcaa tatataacac
     5821 ttcgtatccg tctaatacca acaggttact ggtctcgtcc actattgccc ttaatttgtc
     5881 caaaaacggc atggtaaggt tcaccaggga atattggctt ttggccttgc tgcttattct
     5941 aaacaggccg acacctattc tgtatttctt gttttttttg ttctgctcaa ttatattgtg
     6001 cagctttaat gtattgagaa gatgataggt tgcactgact ccataattca gactgttgct
     6061 tatttctgtt aatccaagtc ctttttctga ttcagccagt tcttccagaa tttcaatggc
     6121 ttttataaca gaaccgatgg tttttgtgtt tttctcgtta ttcttatcca atcctaatca
     6181 gctcctgatg tgcaatatga tataatatgc gcttgcgcat attatatcat attaatgcat
     6241 aaggtgtcat aaaaggaata atattttcat gaatgaaaca ttttttacat ttatgaaaat
     6301 taatattcta aaaatacgcc tttgctgcag acaaggcata gtgcaaatca ggcataaact
     6361 taataaatgc gggcaaggac aagggctatg gcgtgaatta ttaaatctgg catggtaatt
     6421 gcattactat aattaatata tgttttacag gagagcgggg gattaattaa tgttaagcaa
     6481 aaaggtaatg gaaaactata ttcttatctt gaaaagtgaa ttaattgaag cccttggttg
     6541 taccgagccc attgcaattg cttatgcggc agctaaagcc aggtcggtgc tgggaaaagc
     6601 accggaaagg atagaggtag ggtgcagcgg taatattgta aaaaatgtaa aaggtgttgt
     6661 ggtgccaaat accggagggc ttataggcat cgatgctgcg gctgttgcgg gagtcgtggg
     6721 gggagatcct gacaggggac tgcaggtttt ggaaagtgtt aggaggaaac aatactgtac
     6781 tgtaaaaaga ttaaccgagg aaggttactg caaggtttat catctaaagg gagtggacaa
     6841 cctttatatt gaagccaagg tgtatgcggg aaatgaatcc gcagaggttt atattgtaga
     6901 tgcacatacc aacatagtta aaattgtaaa agacggaaag gttctttttg agaataataa
     6961 cggcggccgc aaagactcca aattgtctgg agacaaaacc cttctcaata taaaggatat
     7021 tctggaattt gccgaaacgg ttgatttgga tatactgcat gaactccttg aaaaacaggt
     7081 aacagtaaac acggctattg cggaagaagg catcaggaat gactatggtg ccagcgtcgg
     7141 aaaaacactt ctggaaaact acggcaatga tgtaacagta agggcaaggg ctatggctgc
     7201 tgccggaagc gatgccagaa tgagcggttg ttcactgccg gtagttatta actcaggcag
     7261 cggaaatcag gggattacgg tatctgttcc tgtaattgag tatgcaaggg aattaaagac
     7321 aagtgaagaa aagctcttta gggcattgat aataagcaat ctcgtttcta ttcatcagaa
     7381 agcccaaata gggaatcttt cggcatactg cggtgctgta agtgctgcgg cgggaagcgg
     7441 tgcagggata gcatacctta acggaggcgg ctataaagag atttccagta caatcatcaa
     7501 tacaatagcc aacataggcg gtatggtctg tgacggagca aagccctcct gtgccgcaaa
     7561 aatatcttct gctgtcggag cggcaatttt aggataccag ctaagcgcaa ggggtaaggt
     7621 atttagaagc ggtgaaggtc ttgttaaagg cggagttgag gaaactatca aaagctttgg
     7681 aagagtaggc aaaaagggaa tggaaggcac ggatattgaa ataattaata taatgcttga
     7741 taaataggca aagattaata atcgttgagt tttctataga gagtagtaat gctcattccg
     7801 agcttttctg cgattttcct cttcgtctcg gtagtattgc cgtatttgct tataagcttt
     7861 cttataagct ttttttcata ttctttggta atgtcaccca agctctttaa attatcgatt
     7921 tccggtgatt caatattgat gtattcatca ttatcttttt ttgcgtagtt ggccagaaag
     7981 tattcaggta aattctctgc agtgatttca tcatcaaagg aaaaattaat ggcatattcc
     8041 agtacattct ccagctctct cacatttccc ggccaaccgt agcccatgaa cattgtttct
     8101 acatccttgg aaatgcgctt tggagatttg ttcatcttaa ggcataattt cttgattatg
     8161 tattcggaac acaggaggac atcttcttct ctgtccttta aggacggcag gtgcaggggt
     8221 attacattta acctgtagaa taagtcttcc ctgaattcgt tattccttac catttcctca
     8281 aggtttttat gagtagcgga aattattctg acattaacat ttatttgagc ggagccgcct
     8341 atcctgtcaa tctttttatc ctggagaacc ctgagcagct ttgcctgaag atgcagaggg
     8401 aggtctgcaa tttcatcaag aaacagtgtg ccgttattgg cctgctcgaa cttgcctgtt
     8461 ttgcctttct gacttgcacc ggtaaatgag cctttttcat aaccgaagag ttccgattcg
     8521 ataaggtttt caggcagggc ggcacagttt accgcaacaa aagggccgtc tttcctgtag
     8581 cttgcattgt gtatggatct ggcaaacaac tctttgccgg taccgcttgt accgtgcaga
     8641 aatatcgtgg aatcggattt agcggctatt tttgctttct caattacgtc ctgcattatc
     8701 tgactgcgtc cgattatatt ctcgaaggtc gtttcttcat cgctgttgta agtcatggct
     8761 atgctgatta tttcatccag agtatcaaaa ttaattatag tggagacgtt cttattatcg
     8821 agaattatat aatttacttt atatatgacc tttatcagct tgccccctat tgcccagctg
     8881 cctatgactt ccttattgtt ggtatcctca gctttaaaac ctttaataat gctgccgatt
     8941 cttttgttaa taatcttgtc ggctgaaaaa ttaatattca gggattttat tgctttggaa
     9001 tttatataaa taatccgctg ctccttattc aatatgataa tgcttctgtt tatggagttt
     9061 atgatgccgt ttatttcggc tatgcccata ttgagcttat tcaccatttt gatgcttatt
     9121 atctcttttt cgatggtttc cgcaaaatgc ttaaggattt caagatattc ctcttttttt
     9181 tcaaccataa gctgatcctg ggcggcatta aaggatacaa agcttacgac accgataaca
     9241 tcattttcaa cttttatcgg aagaatcatg cttttaagct ctttgcaggt atcagaattg
     9301 ttgcaggttt gacatatcgg atttttttta tcgtataggt tcaactctgc ttttccggtt
     9361 ttcaatactt ttgcaaataa tgaattttcg gggctgcttt cattaatacg atggtaaaag
     9421 tctcctgttc ctgcgattct cagcaaatgc tcatctacca cggtggtttc tacattaagt
     9481 atggatgaaa gcgactctgc caaggattga attttatcat caataagttt cagatatgac
     9541 acgggcacac cttcctaaaa ccatatttaa ccactgatat ttgcagggta cattaatagc
     9601 ttatcataag atgcctgcaa tggaaagaca tgcggtgatt cattccttga ctttaaatgg
     9661 gatagggcgc ataattaaag tataggtaaa tcataattta ctaatagtaa aaaaggagga
     9721 tttctatgaa aaccaggaaa ctgttgatga ttccgggacc cactcctgta gccaggtcca
     9781 tccaggaaca gatggggaga gagactgttg cttttggaga tccggctttt gtaaaggatt
     9841 ttaaggagct tctggaggat ttgaagtctt tgtttaattg cagcggagaa gtatttgttg
     9901 ttgcaggcac cggcactatg ggtatggaaa tggccatagc caatactaca aaggcaggcg
     9961 acaatgtgct gatagtatct cacgggtttt ttggagaccg gttcatagac ctatgcagca
    10021 gaaaaggttt gaatgcagat gttatctcca gcgaatgggg cgaaacggta cctgttgaaa
    10081 aaattaaaga aaagctgaaa gagaaaaaat acgcggctat gacagttact catgttgata
    10141 cttccacagg ggtttgtgcg ccggttgcgg aaatcggcaa gatgatgaag gaattcccgg
    10201 agacaattta catagttgat ggtgtttgcg ctacggcagg agagcctgaa gatgttgacg
    10261 ccatgggaat agatatattg tttacaggct cacagaaagc cttcggcgtt gcccctggcc
    10321 tgacaatgct ctgggcaagc gggaaagcac tggaaaggcg caaaagcctg ggaagcattc
    10381 ctgagttcta catagatttt gaaaaatggc ttcctgtaat gaaggatacg gccaagtact
    10441 ttgccacacc ggctataaac atgatatggg ctttgaagga atcggtgcgt atcataaagg
    10501 aagaaggaat agaaaacagg tatgcaaggc atagaaaaac cggtacagca atgcaggcag
    10561 cattagaagc cctgggattc agaatattgg caaaggaagg ctgcagggcc gccacattat
    10621 ccaatcctgt ttatcctgaa ggagtagatg atgcaaaatt cagaggactt ctggcagagg
    10681 aaggcatcat ggtggcagga ggactggctg cctacgcagg aagaatgttc aggctgggcc
    10741 atatgggcaa tattgataag catgatatga tatcggttat tgcagccgta gaaagggcat
    10801 tatacagagc aggcatgcct gtggagcttg ggaaaggcgt aggagtagta cagcagcatt
    10861 taattgatag ttaaaagata aacaataata tttctccatt atttattgac agaatattca
    10921 gtaagttata taattaagct accccaagaa aagggacaac cttagtaagg ggggtgcccc
    10981 gcgggattaa gctattgatt acctgctgaa acgaattgat ttgtttcagg aagggtacgt
    11041 gtagtccact gaaaggcctt aaggccgctg ttgtttcgat ttgatcgaac tgtattttca
    11101 gaaacccgct cctttttgaa tgacgattta tataagcagg aattagagct gaagcgatgg
    11161 ggtaaaatta aataaggagg taacctccat tgcaggaaac ctcgggaaat ggataggcaa
    11221 tcgttgtaaa agtatgtcgc ctgtccattt cccatttttg cgtatcatg
//
LOCUS       DAOQUR010000067        11150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_6572_length_11150_cov_10.200090, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000067 DAOQUR010000000
VERSION     DAOQUR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_11150_cov_10.200090"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKI50_10690"
     CDS             <1..789
                     /locus_tag="PKI50_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04206.1"
                     /translation="GFWKLEKSDMVKLYTEGSKVVKIVGESKIRKIVGKLAGIQTGDN
                     TLLKIQMQNGEIRNYTITAKTIVIKDGKSALWQELKAGNDLVVTTSYNELVEINADGV
                     KGSDKGVVESIVFSRIAPPKIVITALDGRQNTYFADSDIEIAGAGEDMYSLRPGMQVD
                     VNLIDDEISKIAVVNETAQVLVDWKGLIKSIDLEAKLVVLEVYDSSTNKYVDRKVYIT
                     DDTKIADADFNFLGLNSLKANQIINVIGTGTADGVSAKAIQVVN"
     gene            850..1722
                     /gene="galU"
                     /locus_tag="PKI50_10695"
     CDS             850..1722
                     /gene="galU"
                     /locus_tag="PKI50_10695"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNT04207.1"
                     /translation="MRVKKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYIVEEA
                     IDSGIEEILIVTGRNKRAIEDHFDRSVELELELARKNNNGLLKQVQDISNLVDIHYIR
                     QKEAKGLGHAIYCARTFVGNEPFAVMLGDDVVSSRIPCLRQLMDVFEDYSNTVLGVQK
                     VSPDDVNKYGIISCEELGERLYKVKDLVEKPAINDAPSNVAILGRYIITPEIFDILGK
                     TEPGSGGEIQLTDALRTLGNSQPMYAYNFEGKRYDIGSKLGFLQATVEFALQREDLKD
                     EFREYLKEVYYGLF"
     gene            1709..3406
                     /locus_tag="PKI50_10700"
     CDS             1709..3406
                     /locus_tag="PKI50_10700"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNT04208.1"
                     /translation="MGYFERYNKWMTDPAFDEETRKELMAIKDDEKEIEDRFYRDLEF
                     GTGGLRGIIGAGTNRMNKYTVGRATQGLANYILRKNIDNPSVVIAYDSRRYSREFAMQ
                     AARVLNSNGIAAYVFSELRPTPELSFAVRELKAAAGIVITASHNPPQYNGYKVYWSDG
                     GQVVSPIAEELIAEANSIQDFSEIKCIDECEIRDKGLLRFLGREMDDRYIEKVKSLSL
                     NSEVVKEMAEDFRIIYTPLHGTGNIPVRSALKAVGFTNVTVVPEQEQPDKDFPTVKFP
                     NPEEHEAFTLAIELAKKQSADIIIGTDPDCDRVGAVVRNTLGEYVVLTGNQTGALLTD
                     YMLGSLKKQGRLNDRSTVINTIVTGKLGEKIAESYNVRTISVLTGFKYIAEKIKEFES
                     SGLEFVFGYEESYGYLAGPFVRDKDAVIASMLICEMAACYKKRGMTLYEGLMSLYRKY
                     GFYKESLTSVTFKGKEGMEEMVSLINALRDKLPGEVSGIKVEEVRDYLDYQGPVRLPK
                     ENVLQFILEDKSWFCIRPSGTEPKLKIYYSVIGNAQEDAEERMARLKAGVEAIVKVK"
     assembly_gap    3658..3757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3769..4140
                     /locus_tag="PKI50_10705"
     CDS             3769..4140
                     /locus_tag="PKI50_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HNT04209.1"
                     /translation="MIRLRMSAHDAHYGGNLVDGARMLGLFGDVATELLIRNDGDEGL
                     FCAYDSVEFLAPVFAGDYIEAEGEIVKVGNTSRKMQFEARKVIQARPDINDSAAEVLK
                     TPVVVCRASGTCVVPKDKQRL"
     gene            4318..5133
                     /locus_tag="PKI50_10710"
     CDS             4318..5133
                     /locus_tag="PKI50_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNT04210.1"
                     /translation="MDKLIITACLTGAEVTREQQPNLPISPDEIAEAAYQAWKAGASI
                     VHVHARKADGTPTQDIEVYREIKEKIAEKCDVIFQPSTGGAVWHSKEERIQPVYLKPE
                     MASLSTGTCNFGPDVFMNTEEYMESFAKIMQENGVKPEVEVFEKGMINNALRLVKKGL
                     LTLPLHFDFVMGVPGAISGEPRDLIYLAESIPEGCTWTVAGIGRAELPLSVMAIVMGG
                     HVRVGFEDNIYYSKGVVAESNAQLVERIARISRECGREVASPNEARQILNIKK"
     gene            5371..5745
                     /locus_tag="PKI50_10715"
     CDS             5371..5745
                     /locus_tag="PKI50_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04211.1"
                     /translation="MLFDTNISAAVKCTHCGRLAIREISLFNLSKDSMLEGSCECGKG
                     LFRIRSSDSKTFCVYINCIACDKEHLYVFNSRQVLSEKVKILSCPNSKLDIAFVGNRN
                     LVREMAVKYQKDLQELIKVLEG"
     gene            5841..7232
                     /gene="rho"
                     /locus_tag="PKI50_10720"
     CDS             5841..7232
                     /gene="rho"
                     /locus_tag="PKI50_10720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT04212.1"
                     /translation="MAGLNELMEKSMTELRELAKSMDIKSYYKFRKDELVNEILKRIQ
                     EAKIAQGIIPPPPKEEKPEEEMPIKRDTSEKPLSDEEKKFLESKDIEKGGRADGILEI
                     LPDGFGFLRSDNYQSGDRDTYISPSQIRRFNLRTGDRITGITRAPKEGEKFPALLYVQ
                     GVNGDNPESVIRRPYFEDLTPIFPNRKIVLESNPKDLSTRIIDLLAPIGMGQRGMIVS
                     PPKAGKTILLKKIANSITANYPKIELIVLLIDERPEEVTDMKRSINGDVIYSTFDEMP
                     ENHCKVAEMVLERAKRLVEQKKDVVILLDSITRLARAYNLTIAPTGRTLSGGLDPGAL
                     YKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEEFKGTGNMEVHLDRKLSEK
                     RIFPAIDMNRSGTRREEMLLTPKEYETIMTIRKAFSSGSSSEVAEYLINKLVHTRTNE
                     EFIEAIKYANFRD"
     gene            7311..7532
                     /gene="rpmE"
                     /locus_tag="PKI50_10725"
     CDS             7311..7532
                     /gene="rpmE"
                     /locus_tag="PKI50_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNT04213.1"
                     /translation="MKEGIHPKYGTATVKCACGETFETGSVKKEIKVEICSKCHPFFT
                     GKQKLVDTGGRVDRFKKRFNLNKEENKEE"
     gene            7591..8172
                     /locus_tag="PKI50_10730"
     CDS             7591..8172
                     /locus_tag="PKI50_10730"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNT04214.1"
                     /translation="MYGPKNHGWIEVIVGPMFSGKSEELIRRINRARIARQKVQVFKH
                     SIDDRYGVDHVVSHNGIKIEAIKVSGSEDIKKYIEDDALVIAIDEVQFFDKGVVDLCV
                     KLADEGKRVIAAGLDQNFRGEPFSPTPELMSVAEFADKIQAICIKCGNPATRTQRMIN
                     GKPASYDDPIILVGAKESYEARCRKCHEVPGKK"
     assembly_gap    8173..8272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8295..9194
                     /locus_tag="PKI50_10735"
     CDS             8295..9194
                     /locus_tag="PKI50_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNT04215.1"
                     /translation="MKPKAVGGQAVIEGVMMKGAEDIAIAVRKPDGEIVVKKEKLKSN
                     RKKISKIPIIRGIFTFVDSLVLGVNALLYSSEFVDVEEEEKKKPSKLDEFLEKNIIWI
                     SVVISVVFSVVLFILLPTVLVGVLKAYTGNTLILNGIEGIVRIAIFLGYILAISGMKD
                     IRRVFEYHGAEHKSIFCYEHGEELTVENVKKYGRLHPRCGTSFLFIVMIVSILLFSLF
                     RWSGLLMRLVIRILLIPIVAGISYEIIKWAGKSESKLSCIVSAPGMWLQKLTTREPDD
                     KQIEVAVEALKNVLVPDPEADNW"
     gene            9194..10087
                     /gene="prmC"
                     /locus_tag="PKI50_10740"
     CDS             9194..10087
                     /gene="prmC"
                     /locus_tag="PKI50_10740"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNT04216.1"
                     /translation="MGSIRESLKKAAYKLSESRGSNTPVLDAELLLLHVLKQSGMDFD
                     RVKLLTQSGYVIDEKTADMYMNLIDERCKGKPVQYIIGRQEFMGFELYIGEGVLIPRA
                     DTETVVEKVIEKAAGMKKPNIIDMCTGSGAIAVSLAMNIPDARVWAADISDSALDCCR
                     INAARYGLEGRIRVIKSDLFENIGGEGLIGNTDVIVSNPPYIESEAIKNLDANVRCYE
                     PHLALDGGKDGLVYYRRIVNDSAEFLKTNGILAFEVGYDQGGKVKNIMEESGHYYVVN
                     IEKDLSGFDRCVWGRKTIFGE"
     gene            10092..>11150
                     /gene="prfA"
                     /locus_tag="PKI50_10745"
     CDS             10092..>11150
                     /gene="prfA"
                     /locus_tag="PKI50_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNT04217.1"
                     /translation="MLDKLESIEEKYEDLSHKISDPEVIAEQSVWQKLMKEHASLEPI
                     VEKFREYKSVLKEIEDSKEMLLDKSDKDFEEMVRLELAELEEKQEKLEEELKILLLPK
                     DPNDEKNVIVEIRGAAGGEEAALFAGDLLRMYMRYAERVGWKVEMLSSNPTDIGGYKE
                     VIFEIEGKGAYSRLKYESGAHRVQRIPTTEAGGRIHTSTSTVAVLPEAEDVDVEININ
                     DLRIDVFRSSGHGGQSVNTTDSAVRVTHLPTGIVVSCQDEKSQLKNKEKAIKILKSRL
                     LDLEIERHNAEIANDRKSQVGTGDRSERIRTYNFPQGRVTDHRIGLTLYQLDSFMDGD
                     LDEMIDALITSAQAEKLKG"
BASE COUNT         3452 a         1960 c         3002 g         2536 t
ORIGIN      
        1 gggttctgga agcttgagaa aagcgatatg gtcaagcttt acactgaagg cagcaaggtg
       61 gtaaaaattg ttggtgaaag caagatcaga aaaatagtcg gaaagcttgc gggaatccaa
      121 accggagaca atacgcttct taaaatacag atgcagaacg gtgagataag gaactatacc
      181 atcacagcca agactatagt gataaaggac ggaaaaagcg ctctgtggca ggagctgaaa
      241 gcgggcaacg acctggtggt aactacttca tataatgaat tggttgagat aaatgccgac
      301 ggagtaaagg gcagcgacaa aggtgtcgtt gagtccattg tattcagcag gatagctcct
      361 ccgaagattg ttataacagc cttggacgga agacagaata catattttgc ggacagcgat
      421 atagaaatag caggagcagg ggaagacatg tattccctga ggccgggtat gcaggtggat
      481 gtcaatctga ttgatgatga aataagcaaa atagcggttg taaatgaaac agcccaggtg
      541 ctggtggatt ggaagggcct tatcaagagt attgaccttg aagcaaagct tgtagtctta
      601 gaggtatatg acagcagtac aaacaaatat gtcgacagaa aggtatatat aacagatgat
      661 acaaagattg ccgatgctga cttcaatttc ctcgggctga acagtctgaa ggccaatcag
      721 attataaacg tcataggtac cggaacagcg gatggtgtgt ctgcgaaagc aatacaggtt
      781 gttaattagc atggggcgaa cagcgttcgc cctttcttta gtatgaaaga aatacaaagg
      841 agaattataa tgagagtcaa aaaggccata atacccgcag cgggattggg aacgcggttc
      901 ctgcccgcaa caaaggcaca gccgaaagaa atgctgccca ttgtagataa accgacaata
      961 cagtacatag tggaagaagc aattgattcg ggaatagaag aaatattgat agtaaccgga
     1021 aggaacaaga gggcaattga ggaccatttc gacaggtcgg tggagctgga gctggagctt
     1081 gccaggaaaa ataataacgg gcttttaaag caagttcagg atatttccaa tttggtagac
     1141 atacactata tcaggcagaa ggaggcaaag ggccttggcc atgcaatata ctgcgccagg
     1201 acctttgtgg gaaacgagcc ctttgcagtg atgctggggg atgacgtggt cagctcccgg
     1261 ataccgtgcc tgagacagct tatggatgtg tttgaggatt attcaaatac tgttctgggt
     1321 gtccagaagg taagccccga tgacgtgaat aagtatggga taatcagctg tgaagaactc
     1381 ggcgaaaggc tctacaaggt gaaggatctt gtggaaaagc ccgcaataaa tgatgctccg
     1441 tccaatgttg ccattttggg aagatatatt ataacccctg agatatttga tatactcggg
     1501 aaaacggaac ccggctccgg aggggagata cagcttacgg atgccctcag gacgctggga
     1561 aacagccagc ccatgtatgc atacaacttt gaaggcaaaa ggtatgatat cggttcaaag
     1621 ctgggcttcc ttcaggcaac agtggagttt gccctgcaaa gggaggattt gaaggatgaa
     1681 ttcagagagt atcttaagga ggtttactat gggctatttt gaaagataca ataaatggat
     1741 gactgatccc gcctttgatg aagaaacccg gaaagagctt atggctataa aagacgatga
     1801 aaaagaaatc gaggacaggt tctacaggga cctggagttc ggaaccggag ggctcagagg
     1861 cataataggc gccggaacca acaggatgaa caaatatact gtgggcaggg cgacccaggg
     1921 cctggctaat tatatattaa gaaagaatat cgacaatccg agtgttgtaa ttgcttatga
     1981 ttcacgccgt tattcccggg agtttgccat gcaggcggca agggtactga attccaatgg
     2041 gattgccgct tatgtattca gtgagttaag gcctacgccg gagctttctt ttgctgtcag
     2101 agagcttaaa gctgctgcgg ggatagttat aactgcaagc cacaaccccc cccagtacaa
     2161 cggctataag gtttattgga gcgatggggg gcaggtggtt tcgccgattg ctgaggagct
     2221 tattgcagaa gcaaattcaa tacaggattt ttccgaaatc aaatgtatcg acgaatgtga
     2281 gatacgggat aagggactgc tgagattttt gggaagggaa atggacgaca ggtatattga
     2341 gaaagtaaaa tccctttcgc tgaattccga agtagtgaag gaaatggcgg aggacttcag
     2401 aattatatat acgcctttgc acggaaccgg caatatcccg gtcagaagcg ctctcaaagc
     2461 cgtcggcttt accaatgtga cggttgtgcc tgagcaggag cagccggata aggattttcc
     2521 cacggttaaa ttcccgaatc ccgaggagca tgaggccttt accctggcta ttgagcttgc
     2581 gaagaagcag tctgccgata tcataatagg aactgatccg gactgcgaca gagtaggcgc
     2641 agtggtcagg aatacccttg gagaatatgt ggtacttacg gggaatcaga cgggggctct
     2701 gcttacagac tatatgctgg gatcccttaa gaagcagggc aggctgaacg acaggagcac
     2761 cgtcataaac accatagtca cgggaaagct gggggaaaaa attgcggaaa gctataatgt
     2821 aaggacaata tcggtgctta ccggcttcaa atacatagct gagaagataa aggaatttga
     2881 aagcagcggg ctggagttcg tgtttggcta tgaagaaagc tacggatatt tggcggggcc
     2941 ttttgtaaga gacaaggacg cagtcatagc atcaatgctt atctgtgaga tggctgcctg
     3001 ttacaagaaa aggggaatga ccctttatga ggggctcatg tcgctgtacc gaaagtacgg
     3061 tttttataag gaaagcctta cttccgtaac ctttaagggc aaggaaggaa tggaggagat
     3121 ggtcagtctc ataaatgccc tgagagataa gttgcccggg gaagtcagcg gcattaaagt
     3181 ggaagaggta agggactatc tggattatca agggcctgtc aggcttccca aagagaatgt
     3241 gcttcaattc atactggagg ataaatcatg gttctgcata aggccttccg gaaccgaacc
     3301 aaaattgaag atttattatt ctgtcatagg aaatgcccag gaggacgctg aggaaagaat
     3361 ggcaaggctt aaagcgggtg ttgaggccat tgtgaaggtt aaataggcaa aaaagaagga
     3421 tatattgact caatagggaa tatatagtaa tacatataat aagaaaactt gggggggtgt
     3481 ggacatgtcg gccaaaaaac tgcttgtggt ggacgatcag cctggagtca ggtttcttat
     3541 ttgtgaaata ttcagggaag aaggatacag tgttgccgag gcgggcaatt ttgaggaagc
     3601 aatagagaca gcaaaaaagt tcatgcctga tgtggctttg gtggacatga gactgggnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg acaaggttat tattaggctg
     3781 aggatgagtg cacatgatgc acattacgga gggaatctgg ttgacggagc gagaatgctg
     3841 ggacttttcg gagatgttgc aacggaactc cttataagaa atgacggaga tgaaggcctt
     3901 ttctgtgctt atgacagtgt tgagtttctt gcaccggtgt ttgcgggaga ctatattgaa
     3961 gctgaggggg aaatcgtcaa ggtagggaat acatccagga aaatgcagtt tgaggccagg
     4021 aaagtaattc aggcaaggcc ggatatcaat gattcggcag ccgaggtact gaagactccg
     4081 gttgtagttt gcagggcaag cggcacctgc gtagttccca aagataaaca gcgattgtag
     4141 ggacaccata catggtgtcc gccatacatg gtgtccgcca tacatggtgt ccgccataca
     4201 tggcatttat attgtagtga cgccatacat ggcatcaatg tcgtaacgac gccaaacatg
     4261 gcatttatat tgtagtgacg ccatacatgg cgtcagatga taaaaggggg attattcatg
     4321 gacaaattaa taataacagc gtgcctgacg ggggcggagg taacaaggga acaacagccc
     4381 aaccttccca tcagccccga tgaaatagcc gaagcggcat accaggcatg gaaggccggt
     4441 gcttccattg ttcacgtcca tgccagaaag gctgacggca ctccgactca ggatattgaa
     4501 gtgtacaggg aaatcaaaga aaagatagca gaaaaatgcg atgttatatt ccagccctca
     4561 acaggaggtg cggtatggca ctccaaggaa gaaaggatac agccggtata cctgaagcct
     4621 gaaatggctt cactcagcac ggggacatgc aatttcggcc ctgatgtgtt tatgaacact
     4681 gaggaatata tggagtcctt tgcaaaaata atgcaggaaa acggcgtaaa gcctgaggtt
     4741 gaggtattcg aaaaaggtat gattaataat gccctgagac tggtcaaaaa ggggctcctc
     4801 acgctgcctc tgcattttga ttttgtcatg ggggttccgg gggcaatttc cggtgaaccc
     4861 agggatctga tatatctggc ggaaagcatt cccgagggct gtacctggac ggtggcgggt
     4921 ataggaaggg ctgaactgcc cttgagtgta atggcaatag taatgggagg ccatgtaaga
     4981 gtgggctttg aagataatat atattattct aaaggtgtgg ttgcggaatc aaacgctcaa
     5041 ctggtggaaa ggattgcaag gatatcgagg gaatgcggcc gtgaagtggc atcacccaat
     5101 gaagcaaggc aaattctgaa tataaagaaa taattaaaag aaacgggtaa cactgaagac
     5161 acagaaatcc actgattcca cggcggaata ttatattttt atccgtgcta ttcagtggat
     5221 tatccgtgtg ttcagtggtt tccaagccta tacctataag gaataccaag gttttcttga
     5281 gtctaaaaaa aggatttttt aaattaatgt agaattataa gaatagctta gtgttaacta
     5341 ggcattatta ttcacacggg gggtgagaac gtgctttttg atacgaatat aagtgcagct
     5401 gtaaaatgca cccattgcgg aaggctcgca atcagggaga tatccctttt caatctgtca
     5461 aaggacagca tgcttgaggg aagttgcgag tgcggcaaag gtttattcag aataagaagc
     5521 agcgattcaa agaccttctg tgtttatatt aattgcatag cttgtgacaa agagcatctt
     5581 tacgttttta attcaaggca ggttctcagt gagaaggtaa aaatacttag ctgcccaaac
     5641 agcaaattgg atatcgcatt tgtgggcaac aggaacctgg tcagagaaat ggctgtaaag
     5701 taccagaagg atctgcagga gcttatcaag gtacttgagg gataaacaga ggcacgggtg
     5761 tggttttttg cccgtgcctg atttttacaa gccagctgat gtattattta gcgataactg
     5821 gaaataatta ttaagaggtg atggcaggat tgaacgaatt aatggaaaaa agcatgactg
     5881 agctaagaga gcttgcgaag tccatggaca tcaagagcta ttataagttc aggaaggatg
     5941 agctggtaaa cgagatattg aaaagaatcc aagaggcaaa aatagcccag ggaataattc
     6001 cgccccctcc aaaagaggaa aagcctgaag aagaaatgcc tatcaaaaga gatacttcgg
     6061 aaaagccttt aagcgatgaa gaaaagaaat tcctggaaag caaggatatt gaaaagggcg
     6121 gcagggctga cggcatactt gagatacttc ccgacggttt tggctttctc aggtcggata
     6181 attaccagtc gggggacaga gacacataca tatctccatc ccaaatcagg cgattcaatt
     6241 tgagaaccgg cgacaggata acaggaataa ccagggctcc caaggaagga gagaaatttc
     6301 cggcacttct gtatgtgcag ggagttaatg gggataatcc tgaaagtgta atcagaagac
     6361 catatttcga ggatctcact cctatatttc ccaacagaaa aatagttttg gaaagcaatc
     6421 ctaaagattt gtcgacaaga attattgatc tcctcgcacc tataggtatg gggcagagag
     6481 gcatgatagt atcacctccg aaagcaggaa aaacaattct tttaaaaaaa atcgccaaca
     6541 gtattacagc aaattatcct aaaatcgaat taatagtttt gttgatagat gagaggccgg
     6601 aggaagtaac cgacatgaag agatccatca acggcgacgt gatatattcc acctttgacg
     6661 aaatgccgga aaaccactgc aaggttgccg agatggtgct ggaaagggca aagcgccttg
     6721 tcgagcagaa gaaggatgtg gttattctcc tggacagtat cacaagattg gcaagggcat
     6781 ataatctgac catcgcacct accggaagga ctctttcagg agggcttgat cccggagctt
     6841 tatacaagcc caagaagttt ttcggagctg ccaggaatat agaagaaggc ggcagcctga
     6901 ctatacttgc gactgccctt gtggataccg gaagcaggat ggacgatgtg atatatgaag
     6961 aattcaaggg tacgggcaac atggaggtac atttggacag aaagctttcg gagaagagaa
     7021 tattccctgc aatagatatg aaccgttccg gtaccagaag ggaagaaatg cttctgactc
     7081 caaaggaata cgaaaccatt atgactataa ggaaagcctt cagcagcggt tccagcagcg
     7141 aagttgcgga gtatttgata aacaagcttg tccataccag gaccaatgag gaattcattg
     7201 aagcaataaa atatgcaaat ttcagagact aaaaaataat tgccaagtat aaaatactgt
     7261 gttaaaatat atacttgcag atgtaatcca taaagaaaga ggtgaataga ttgaaagaag
     7321 gaatacatcc aaaatatgga actgcaacag ttaaatgtgc ttgcggggaa acattcgaga
     7381 caggctcagt aaaaaaagaa ataaaagtag aaatttgttc caaatgtcat cctttcttta
     7441 caggaaagca gaagcttgta gatacaggcg gacgtgttga caggttcaag aagagattca
     7501 acttgaataa agaagaaaac aaagaagaat aattaataga ggttaaagct ttctttaacc
     7561 tctaatttca tatgggggtc ggattgaatt atgtatgggc cgaaaaatca cggatggata
     7621 gaagtaattg tagggcctat gttcagcggc aaaagcgagg agttgattcg aagaatcaac
     7681 agggcgagaa ttgcaagaca aaaggtccag gtattcaagc acagtattga tgaccgttat
     7741 ggggttgatc atgtggtgtc ccataacgga atcaagatcg aagcaatcaa ggtttccggt
     7801 tccgaggaca ttaagaagta tatagaagat gacgctcttg tcatagccat agatgaagtg
     7861 cagttctttg ataagggtgt agtggatttg tgcgtcaaat tggccgatga aggaaaacgc
     7921 gtaattgcgg cgggacttga ccagaatttc aggggagagc cctttagccc tactccggag
     7981 cttatgtccg ttgcggaatt tgcggacaag attcaggcta tatgcatcaa gtgcggcaac
     8041 ccggccacca ggacccagag gatgataaac gggaagccgg cgtcctatga tgatcccatt
     8101 atactggtag gagccaagga aagctacgaa gccaggtgca gaaagtgcca cgaggtgccg
     8161 ggaaaaaagt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgaaaggt
     8281 agaaatttat gaatattaag ccaaaagccg taggaggcca ggcggttatt gaaggcgtaa
     8341 tgatgaaggg cgccgaggat attgccattg ccgtgcggaa gcctgacggt gaaatagtag
     8401 tgaaaaagga aaagctgaag agtaatagga agaaaatatc caagatacct ataatccgag
     8461 gcatattcac atttgtggat tcattggtgc tgggggtcaa tgccctcttg tattcctccg
     8521 aatttgtcga tgtggaagag gaggagaaga aaaaaccctc caagcttgat gaattccttg
     8581 agaaaaacat aatatggata tccgttgtta tttcagttgt gttctcggta gtccttttta
     8641 tattgcttcc taccgtactg gtcggcgtat taaaggctta taccggcaat acccttatac
     8701 ttaacggaat tgagggtata gtaagaatag ctattttcct gggttatatt cttgccatat
     8761 ccggaatgaa ggatatcagg agagtatttg agtatcacgg agcggagcac aaaagcatat
     8821 tctgctatga gcacggagaa gaactgaccg tggaaaacgt gaaaaagtac ggcaggcttc
     8881 accccaggtg cggtaccagc ttccttttca tagtaatgat agtaagcata ctcctctttt
     8941 cattgttcag atggtcgggg ttattaatgc ggcttgttat aaggattttg ctgataccga
     9001 tagtagccgg aatatcctat gagataataa aatgggcggg caagagtgag agcaagcttt
     9061 cctgcatcgt atccgctccg ggaatgtggc tgcagaagct gaccaccagg gagccggacg
     9121 acaagcagat agaagtggcg gttgaagctt tgaagaatgt tcttgtacct gacccggagg
     9181 cggataattg gtagtgggga gcattaggga atcattaaaa aaggcagctt ataagctttc
     9241 ggagagcagg ggttcaaata ccccggtgct tgatgcggag ttgctgcttc tgcatgtact
     9301 gaagcaatcc ggcatggatt ttgacagagt aaagctcctg acacaatccg gatatgttat
     9361 tgacgaaaag actgccgata tgtacatgaa ccttattgat gaaagatgca aaggcaagcc
     9421 ggtacagtac attataggca ggcaggagtt tatgggcttt gaactgtata taggggaagg
     9481 ggttctgata ccccgtgccg ataccgaaac agttgtggaa aaggtaattg agaaggccgc
     9541 cggaatgaaa aaaccgaata taatagatat gtgcaccggc agcggtgcga tagctgtaag
     9601 ccttgcgatg aacataccgg acgcaagggt gtgggcggcg gatatctccg atagtgccct
     9661 tgattgctgc agaatcaatg cggcaagata cggccttgag ggcagaataa gagtaattaa
     9721 gagtgatttg tttgagaata tcggggggga agggctgata ggcaacacag atgttattgt
     9781 gtccaatcct ccttatatag aaagtgaggc cattaaaaat ctggatgcaa acgtcaggtg
     9841 ctatgagcct catttggctc ttgacggcgg caaagacggg cttgtatact atagaagaat
     9901 tgtgaatgac agcgcagaat tcctgaaaac taacgggata cttgcctttg aggtcggata
     9961 tgatcagggg ggcaaggtga aaaatataat ggaggagagc ggtcattatt atgttgtaaa
    10021 tatagagaag gatctttcag gttttgatag atgcgtctgg gggcgcaaaa caatatttgg
    10081 agagtgatag gatgcttgac aaactggaat ctattgaaga gaaatatgag gatttaagcc
    10141 ataagataag tgaccctgaa gtgattgccg aacaaagcgt ctggcagaag cttatgaaag
    10201 agcatgcaag ccttgagccc attgttgaga agttcaggga atacaaatcc gtgttgaagg
    10261 agatcgaaga ttcaaaagaa atgcttctgg ataagtccga caaggatttt gaagaaatgg
    10321 taaggcttga gctggcggag ctggaggaga agcaggaaaa gctggaagaa gagcttaaga
    10381 tacttctgct cccgaaggat cccaatgatg aaaagaacgt aattgttgaa ataagaggtg
    10441 ctgccggagg ggaagaagct gcgctgtttg ccggagacct gctgaggatg tatatgcgat
    10501 atgctgaaag agtgggctgg aaggttgaaa tgctaagctc caatcccacg gatataggcg
    10561 gttataagga agtcatattt gaaatagagg gaaagggcgc atacagcagg ctcaaatatg
    10621 aaagcggcgc ccacagggtg cagaggattc ccactactga ggccggcggc aggattcata
    10681 cttcaacctc aactgttgcg gtgcttccgg aagcggaaga cgtagacgtg gaaataaaca
    10741 taaacgatct gaggatagat gtgttccgtt ccagcggaca cggaggacag agcgtaaaca
    10801 ccacagacag tgccgtacgg gtgacacacc tgcctacggg gatcgttgtg tcctgccagg
    10861 atgagaagtc gcagcttaag aataaggaaa aggccataaa gatattgaag tccaggctgc
    10921 tggacctgga aatcgaaagg cataatgccg agatagcaaa cgacaggaag agccaggtag
    10981 gcacaggaga cagaagcgaa agaatcagga catacaattt ccctcaggga agggttaccg
    11041 atcacagaat tggactgact ttataccaat tggattcctt tatggacggg gatctggacg
    11101 aaatgataga tgcgcttata acaagcgcac aggcagaaaa gcttaaaggc
//
LOCUS       DAOQUR010000068        11076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_6623_length_11076_cov_9.913982, whole genome shotgun sequence.
ACCESSION   DAOQUR010000068 DAOQUR010000000
VERSION     DAOQUR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11076
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6623_length_11076_cov_9.913982"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PKI50_10750"
     CDS             complement(<1..1170)
                     /locus_tag="PKI50_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNT04218.1"
                     /translation="MGKPRVEYNGKKLEEQLGSKAVVLICLLVLNEKRYMSREKLEGY
                     LWPDSSTDAARYNLRYNLWLIKKNIGTDHKGHKFLHVDSDCCSINKEYEFECDTFEIM
                     SFKHSQNDSLESILKLKQLFRGDLLEGCYFKNCDELNDLIIFERMKYEQRKVKILKSL
                     VDYYEKEESFDNCLEVINEILEIEPYDEEMVLKILDIYVKCGKPVAAITYYNNFSKLL
                     TSSLGISPSNQLRKKYEEIRSVLSDSIDNNSSKANMDTFMKDISKQENVSVIEIISDC
                     IKNVDYFWISDVVGKIIAAVGTDYLNQLSEKELLDLAYIQCDILYYCDDKFEHIKDYG
                     RDTMSVCIINAFIKLLRIVCSKQELTIIILNNTDMDDTSLNVLKYLKRIKIEGLKL"
     gene            complement(1208..2740)
                     /locus_tag="PKI50_10755"
     CDS             complement(1208..2740)
                     /locus_tag="PKI50_10755"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNT04219.1"
                     /translation="MHASILIKNGRCMTMEGKEEKKWIAVEQNKIVAMGDNTEYESLI
                     DGDTIVFDAGGNTVLPGFIDSHFHVIQTALDSISVDLSYAKTYEELGEQLREAASSFT
                     GGYIRGIRISEEQFTEGRFPGRTLLDKYCSNVPVVLNSMEYQVSILNTYAMLYFKIPF
                     TAEGIELDRKQMPTGIFRKQANAILRTNILKNISDNIRKDAVEGIMGTLLMNGITTIN
                     AMEGGFMYSDKDADFLYEHGKEFPIDIELFYQTMDIEKIKAMGLKRLGGCLYVDGTFG
                     ARTAALSFQYADCPGTMGSLLLTQQEINDFVLNCYKNNLQLALYTIGDRAIEMAINAH
                     ENAFYQTGIIGLRHRLEHAELINEKQIQRAKELGILLSMQPSYETYWGGPGKMYEQRL
                     GDKYILTNPFKEIIEKGVIICGGSDSDVTEPRPLLGIHSAVNHPVVKHRVDIYEALKM
                     FTCNGAYGIFQEKNKGTLSVGKTADIIILDRDILLVPSHQIEKARVCVTIKSGETLYS
                     NL"
     gene            2992..3444
                     /locus_tag="PKI50_10760"
     CDS             2992..3444
                     /locus_tag="PKI50_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT04220.1"
                     /translation="MERIVKGGIYHVIARGNNKEYIFKENVDKGYFIKQIKETTAGMG
                     YKVFGFVLMDNHYHIIVQTFDKKLQDIMHQINNKHSKYFNYKYKRVGHVFQGRYKAIL
                     VQDEKYIVGLLRYVHQNPVEAHICNDMDKPGRLLSVKVRFKEKNIDSI"
     assembly_gap    3592..3691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3968..4462
                     /locus_tag="PKI50_10765"
     CDS             3968..4462
                     /locus_tag="PKI50_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT04221.1"
                     /translation="MSENKTLDNLMAAFAGEAQANRKYTAYAKKAEKEGKMNAAKLFR
                     AAADAETLHALKHFEVAGKIGSTAENLKDGVAGETYEYKDMYPDFVKVAEAEGNKAAL
                     MSFTFAMKAEEVHAGLYQDALENIDETEEVFYYLCPVCGNIEKSRPEKCSICGVSGEK
                     FIKY"
     gene            complement(4548..5834)
                     /locus_tag="PKI50_10770"
     CDS             complement(4548..5834)
                     /locus_tag="PKI50_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT04222.1"
                     /translation="MSIRFKLPLLVLAAFLINIMLLYGYYNFYLSREIAGYNSNMREQ
                     LQTETDRIIKRIESSQDFSQALQIISQTENLNIQVADESGRTVFHGGKDGGVSIEINS
                     SGIFHHDNKVYLLKVTQPVSLKKISSYYVVWNLFIAEIFIICSILLFIAAIIYFNYVK
                     PIIALQKSMERYKEGIHPQNVARKDEIGLLQNRFVRLTEAIEKEKQKQNLIIASISHD
                     IKTPLTSVMGYAERLKKNNLTADRYGRYIDIIYNKSVSIRDLIEEFDEYLNLQMQSGL
                     KQQRISAEKFCAILKSDYEEELAERGVSFSISVSCPDKILFIDIAKMRRVFGNIIGNS
                     LKHFSVREPSIAVFCSKQENCIIFSVEDNGTGVSEGELQKMFDPFYTSDKSRSVAGLG
                     LSICREIVEACNGSIWAENNESGGLCVKIILPHAAP"
     gene            complement(5824..6519)
                     /locus_tag="PKI50_10775"
     CDS             complement(5824..6519)
                     /locus_tag="PKI50_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT04223.1"
                     /translation="MEKILIVDDDREIASLISDALTDEGYQCILAFDGEHAFTLLKSI
                     QDFSLIMLDIMMPGIDGLEICREIRKTVSCPILFVTARNRTYDAMLGFEMGADDYISK
                     PFVVEELVARVKAHLRREKRSLQKNNSIMTIGGISINPESYEVTVNGRAVELSMREFQ
                     LLHYLFINAGKVVSKEQIFSAIWGADFTDIGTVAVNIKNLRDKIDKDNRYIKTVWGVG
                     YKLIKHQEKENEY"
     gene            complement(6524..7711)
                     /locus_tag="PKI50_10780"
     CDS             complement(6524..7711)
                     /locus_tag="PKI50_10780"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT04224.1"
                     /translation="MFLRHKKSILIGIGILIMLTAAVIISFNAAGADRAQSFETVSSS
                     SEQDNRLDFWGEVKYERIYDISIDFPAIVTDIKVKEGDHVSLDQVLVTLDMSEYLGTV
                     EKLQHQLEAGQAGLQSIRKDTGALEADIQQIQEDILEKTEELAKETNADLKILKTSLN
                     LAKKEAENAKRDVSSNQALYDAGAVSGNTLDRYMDILAEKEKALKDIETGIEKTKSAL
                     RTELDQLSILLKSKSAQLKEIKDSNISNTARQESGVAVSRIDLEIMKSKSIKDYLDSD
                     QVISCVPNGIVRNIEVVNGTRLGVQNNPTRVLQLIDADSIIVIAEVEEEFIRNIALDD
                     SVEIVPASDSSTSIPGTVIQISNEAVEKDGKRIIKVQIKPEDPGMVLKPGYSVDVYAN
                     RKE"
     gene            complement(7740..10049)
                     /locus_tag="PKI50_10785"
     CDS             complement(7740..10049)
                     /locus_tag="PKI50_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT04225.1"
                     /translation="MVMQSLFIKSLRDMQKSKVQFLSILIMATLAVCIVTGIDSIWKT
                     VELHSESMYAAANISDLWVNVLNPTEKELWGISRIEGVEKAEKRFVLDALSDLENSPT
                     LRVYAVSGKGVLDRPQLLSGKLSSRSGAILDEVFARMHGLDIGDDISIKLNNKWIRFP
                     IQGLALSSEHIYSVKGSTDTFPDPKKYGFIIINEDMLKNAYGRKIYNQISVKLSPGAD
                     ITKVKTHIFNAVGDDLAGIVAREDHRSVSNINANIQQFKLLAAVFSLMFFIVTALITQ
                     STMMRLVENQRGQIGILKALGYGKKSILWHYTSYGVYMGLLGAFLGLLSGPVIFGKML
                     IPWLRLNFTDYKISINHSNFIISLVLILLCTGGISLYSCLRLLGDSPSVLLRIKPPKE
                     GSHIFLEYLSGLWSRMRFSRRLIARNTSRNKIRLIMSILGITGCTGLIVAAFTIINMI
                     NGIARHTYNVTYTYDHKILLDSKADSRFIHNMGLEGTVQQIRETAAEIICPNGERQLE
                     LLTVSPQKSPLIRLKDVEGNPVLLPDEGAAITRKLADTLKIKAGDIIELKRTNKGYIR
                     VPVKQIVYLATGQGMYMSDTYYESIGEIFKPTAILVKWDNGPDWSFLRGDYAEEYVSR
                     ADQIADVKSSTRVVYIAGVMLIIMGGILAFVVLYNSSILNFSERIRDLTTLKVLGFYQ
                     KEIRSLVLTENTLSVIAGLILGMPVGKVLAEIVAKGLNDQMDLIGNVSFATVVFSVAI
                     TAVFALFINSIAAVKMKSIDMIESLKSVE"
     gene            complement(10043..10744)
                     /locus_tag="PKI50_10790"
     CDS             complement(10043..10744)
                     /locus_tag="PKI50_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04226.1"
                     /translation="MSFITFENVEREYHVGGNIIKAVDGVSFGIEKGTVNVVLGQSGA
                     GKTTVLNLLGGMDSPTKGRIIADNLEISGMAEYELTTYRRNKIGFVFQFYNLVPNLTV
                     LENVLLAEEICKDPLDAKEMLIKVGLKDRMDNFPSQISGGEQQRVSIARALAKNPQIL
                     LCDEPTGALDSATGRIVLKLIRDAARDMGKTVIIVTHNVLISEMADVVLHLKDGKISD
                     IRYNSSPKAAEEIIW"
BASE COUNT         3050 a         2387 c         2100 g         3439 t
ORIGIN      
        1 tagctttagc ccttctattt taattcgttt aagatatttt agtacattaa gggaggtatc
       61 atccatatcg gtattgttca gaattatgat cgtaagttct tgtttactgc agacaattct
      121 taatagtttt ataaaagcat ttattatgca tacgctcatt gtgtcccgcc cataatcctt
      181 tatatgttca aacttatcgt cacaataata cagaatgtca cattgtatat atgctagatc
      241 cagtaattct ttttcgctca attgattcaa ataatcagta cctacagctg cgattatttt
      301 gccaactaca tcggagatcc aaaaataatc tacgttttta atacaatcgg aaataatctc
      361 tataaccgaa acattctctt gtttactaat gtctttcatg aatgtatcca tatttgcttt
      421 agaactatta ttatcaatag aatccgataa tacggacctt atttcttcat acttttttct
      481 aagttgattt gaaggagata tcccaaggct gcttgtcagc aatttgctga aattattgta
      541 atatgttatt gcggcaaccg gttttccgca ctttacataa atatcaagta tttttagcac
      601 catttcctca tcatagggtt caatttcaag gatctcattt attacttcaa gacagttatc
      661 aaagctctcc tctttttcat agtagtccac caaactcttc agtattttaa cttttcgctg
      721 ttcatatttc attctttcaa atatgataag gtcgttgagt tcatcacaat tcttaaaata
      781 acagccttcc agcaaatctc ccctgaataa ttgtttgagc tttagaatac tctccaaaga
      841 gtcattttgg ctgtgtttaa agctcatgat ttcaaaggta tcacattcaa attcatattc
      901 tttatttatg gaacagcaat cactatctac atgcaaaaat ttatgcccct tatgatcagt
      961 tcctatattt ttttttataa gccacaaatt atatcttaaa ttataccttg ctgcgtccgt
     1021 actgctgtct ggccagagat aaccttcaag cttctctctg ctcatatatc ttttttcatt
     1081 taacacgaga aggcagatta aaacaacagc cttactgcct aactgttctt ccagtttttt
     1141 accgttatat tcaactcttg gcttacccaa aaaattaata ctcagcataa tatttcacca
     1201 atccctatca aaggttacta tataatgttt cacctgattt tatagttacg cagactcttg
     1261 ctttttctat ttggtgtgac ggaactaaaa gtatatctct atccaatatt ataatatctg
     1321 ctgttttccc tacacttaaa gtgcccttgt ttttctcttg aaaaatgccg tatgcgccat
     1381 tgcatgtaaa cattttcaat gcttcataaa tatcaactct atgctttaca accgggtgat
     1441 ttactgccga gtgaataccc agtaagggcc gtggctccgt aacatcacta tcggagcctc
     1501 cgcatattat tacacctttt tcaatgattt ctttaaatgg gtttgtaaga atatatttgt
     1561 ctcccagcct ttgttcatac atctttcccg gacctcccca ataggtttca taggaaggtt
     1621 gcatagaaag aaggattcct aattctttag ctctttgtat ttgtttttca tttatcaatt
     1681 cagcgtgttc caaacgatgg cgaagtccaa taattccggt ctgataaaat gcgttttcat
     1741 gggcatttat agccatttct atagccctgt caccgattgt gtataaggca agctgaaggt
     1801 tgtttttata acagttcaaa acaaaatcgt tgatttcctg ctgagttaga aggaggcttc
     1861 ccatggtacc cggacagtca gcgtattgaa aagaaagtgc tgcggttctg gcgccaaaag
     1921 tgccatcgac ataaaggcat cctccaagtc gctttaatcc catggcttta attttttcaa
     1981 tgtccatagt ttggtaaaat agttcaatat ctataggaaa ctctttgcca tgctcatata
     2041 ggaaatctgc atctttatca ctgtacataa atccgccttc catggcgtta atggtagtta
     2101 tgccattcat gagaagagtt cccattattc cctcaactgc atctttcctg atgttatctg
     2161 aaatattttt tagtatattt gtacgcaaaa tagcattggc ttgtttccta aaaatacctg
     2221 tgggcatttg ttttctatcc agttcaatac cttcagctgt aaaaggtatt ttaaaatata
     2281 gcatagcata ggtgtttaat atgcttacct gatattccat ggaattaagt acaacaggaa
     2341 cattactgca atacttgtca aggagcgttc tcccggggaa tctcccctct gtaaactgtt
     2401 cttccgatat acggattccc ctgatatagc ctccggtaaa agaggatgct gcttctctta
     2461 gctgttcccc caactcttca taggtttttg catagcttaa atctacactt atagagtcaa
     2521 gtgcagtttg aatcacatga aaatgactgt cgataaatcc gggaagcaca gtatttcccc
     2581 ctgcgtcaaa cacaatcgta tccccgtcaa taagagactc atattctgtg ttatccccca
     2641 tagcaacgat tttgttttgt tcaacagcaa tccatttctt ttcttctttt ccttccatag
     2701 tcatacatct tccgttttta atcagaatac ttgcatgcat agggtctcct ccaagtttgt
     2761 aagtaattta tccattcatt tttattgtat cataaatttc cattgtaaac atttacaagt
     2821 tgcctgcttc atcaaggaag caaaagaaac tttacagagg agtaagtacg aatatgcaga
     2881 tgtccggtga gttatggtgc caggcacgac agatagacag ttattgacag gaataaatcc
     2941 ttatggtaaa atatggatat agttactggg aggatttttt atggggagac cgtggagaga
     3001 atagttaaag gcgggatata tcatgttatt gccagaggta ataataaaga gtatattttc
     3061 aaagaaaacg tagataaagg atattttata aagcagataa aagagacaac tgcaggaatg
     3121 gggtataaag tgtttgggtt tgtgttgatg gataatcact atcatataat tgtccagaca
     3181 tttgacaaaa agctgcagga tataatgcac cagataaata ataaacacag caaatacttc
     3241 aattataaat ataaaagggt agggcatgtc tttcaaggta ggtataaggc aatactggtg
     3301 caagatgaaa agtatattgt aggattatta aggtatgtac atcagaaccc agtggaagct
     3361 catatatgca atgacatgga taagcctgga agattattat ctgttaaggt caggttcaag
     3421 gaaaagaaca ttgacagcat ataaattgga gtatataaga aaggcattga aactgaatta
     3481 cacatgcaaa gagataggtg agtatataag agcaacgtaa agtgcaatca aagaattgat
     3541 agccaagtac agtagataac tgtctaactg tcgtgcctgg caccacatgc annnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naactgtcgt gcctggcacc acatgcatgt
     3721 agcatccggc tcaattatag ttaattaggc gaatggacag aagaatggac agaatagtaa
     3781 gaaggatagg gtcagacaaa taaagtctga ctttttttct ggaaacggtt gcgtttttta
     3841 ttataaagaa ttataattaa tatataatca ttattgatag gtctttggtt atttcactat
     3901 attgaaacat attgtagaaa agcttttgaa aacatataat tatagtaata ataaggagga
     3961 taaaaaaatg agtgaaaaca aaacccttga caatttaatg gcggcatttg ccggagaagc
     4021 ccaggctaac agaaagtata cggcatacgc aaagaaagct gaaaaagaag ggaaaatgaa
     4081 tgcagccaag ctgttcagag cggcggctga cgccgaaacc cttcatgcct taaagcattt
     4141 tgaagtggcg ggaaaaatcg gttctacggc agaaaacctc aaagatggtg ttgcgggaga
     4201 gacctatgag tataaggata tgtatcccga ttttgtgaaa gtggctgaag ctgaaggaaa
     4261 taaagctgcg ctcatgtcct ttacctttgc aatgaaagcg gaagaggttc atgccggact
     4321 ttatcaggat gcattagaaa acatcgatga gactgaagaa gttttctatt atctctgccc
     4381 ggtctgcggg aatatcgaaa aatccaggcc tgaaaaatgc agtatttgcg gcgtatcagg
     4441 agagaagttt attaaatact aggtgccggg cgctgtgagg tacatttaat acaccttctg
     4501 atgatattat atcagaaggt gtattattcc ggcaggggcg aaatatatca cggggcagcg
     4561 tgaggaagga tgattttaac gcagagccct ccggattcat tattttccgc ccatatcgaa
     4621 ccattgcagg cttcaacaat ttccctgcat atggataaac ccagccctgc tacggagcgg
     4681 cttttatccg aggtgtaaaa gggatcgaac attttttgca gttccccctc tgaaacaccc
     4741 gtgccgttgt cctcaaccga aaatattatg cagttttcct gctttgagca aaatactgca
     4801 atggagggtt ctctaacact gaaatgcttc aggctgttgc cgataatgtt cccgaatacc
     4861 cttctcatct tagcaatatc tataaacagt attttatcgg ggcagctgac actgatggaa
     4921 aaagaaactc ctctttccgc caattcttct tcgtagtctg attttaagat ggcacagaac
     4981 ttttctgcgg atatcctctg ctgctttaac ccgctctgca tctggaggtt aaggtactcg
     5041 tcgaattcct ctatcaaatc cctgattgaa actgatttat tataaattat atcaatatac
     5101 cttccatatc tgtctgcggt aagattgttc ttttttaacc gttcggcata acccatgaca
     5161 gaagtgagag gtgtcttaat atcatgggaa atggatgcaa taataaggtt ctgcttctgc
     5221 ttttcttttt caattgcttc cgtaagcctt acgaaccggt tttgcagcag acctatctca
     5281 tctttgcgtg ccacattctg aggatggatt ccttccttat acctttccat gcttttttgc
     5341 agggctataa tgggcttgac ataattaaaa taaataatag cagcaataaa taataaaata
     5401 gagcatatga tgaatatttc cgctataaac aaattccaaa ccacataata tgaggaaatc
     5461 tttttcaggg ataccggctg agtaaccttc agcaaataga ctttattatc atggtgaaat
     5521 ataccggagg aattaatctc tatgctcacg ccgccgtctt ttcccccatg gaataccgta
     5581 cggccggact catccgcaac ctgaatattc agattttctg tctgagaaat aatttgaagc
     5641 gcctgtgaga aatcctgact gctttcgatt ctttttataa tccggtcagt ttcagtctgc
     5701 agctgctccc gcatattgct gttgtagccg gcaatttccc gggaaagata aaagttataa
     5761 tacccgtaaa gcagcattat attgataaga aaagcagcaa gaacaaggag gggaagcttg
     5821 aatctaatac tcattttctt tctcctggtg ttttattaat ttatatccta cgccccatac
     5881 ggttttgatg tacctgttat ccttgtcgat tttatcccgt aagttcttaa tattcaccgc
     5941 tacagtacca atatccgtga aatcagcacc ccagatcgca ctgaaaatct gttcctttga
     6001 gacgaccttt cctgcattaa taaaaagata atgcagcagt tgaaattccc tcatggataa
     6061 ttccaccgcc ctgccgttta cggtgacttc atagctttcc gggtttattg aaatgccgcc
     6121 tatagtcatg atactgttgt tcttttggag gctgcgcttt tcgcggcgca aatgagcctt
     6181 taccctggcg accaattcct ccacaacaaa gggctttgat atataatcgt ccgcacccat
     6241 ttcaaagcca agcatcgcat cataggtcct gtttctggcc gtcacgaaaa gtatggggca
     6301 ggatactgtt tttctgattt ccctgcatat ttccagtccg tctatgccgg gcatcataat
     6361 gtccagcatt atcaatgaga agtcctgaat actcttcagt aaagtgaaag catgttctcc
     6421 gtcaaatgcc agaatgcact ggtatccttc atcggtaagg gcatcggata tcagtgatgc
     6481 gatttccctg tcatcatcaa caatcaatat tttttccatg atttcactcc ttcctgttag
     6541 cataaacatc cacgctgtaa cccggtttca gtaccatccc gggatcctcc ggcttaatct
     6601 gtacctttat aatccgtttg ccgtcctttt ccacagcttc atttgaaatc tggataacgg
     6661 tccccggtat cgaggtgctg ctgtccgacg ccggtacaat ttcaacagaa tcatcaagag
     6721 cgatattcct gataaattcc tcttccacct cagcaatgac tattatagaa tccgcatcaa
     6781 tcagctgaag aacccttgtg ggattattct gtacccccag ccttgtgccg tttacaactt
     6841 caatattccg tacaatgccg ttcgggacac atgatatgac ttgatcagag tccaggtagt
     6901 ccttaatgct tttgcttttc ataatctcca ggtcaatacg ggatactgcc acaccgcttt
     6961 cctgccttgc cgtgtttgag atgtttgagt cttttatttc cttcagctgg gcactcttgg
     7021 attttaatag tatgctcaac tggtcaagct cggttcttaa agcgcttttt gttttttcaa
     7081 tgccggtctc aatatccttc agggctttct ccttttcagc caatatatcc atgtaccggt
     7141 ccaatgtgtt cccggagact gctcccgcat catataatgc ctgattgctg cttacatccc
     7201 tttttgcatt ttccgcttct tttttcgcca ggttcagtga ggttttcagt attttaaggt
     7261 ctgcattggt ttccttggcc aattcctctg ttttttcaag tatatcctct tgtatctgtt
     7321 ggatatccgc ttcgagagcc ccggtatcct tcctgatgct ttgaagccct gcctgtcctg
     7381 cttccagttg atgctgaagc ttttccacgg ttcccagata ttccgacatg tccagtgtaa
     7441 ccagtacctg atccagggat acatggtctc cctctttaac tttaatatcc gttactatgg
     7501 caggaaagtc aatacttata tcataaatcc tttcatactt tacttctccc cagaaatcca
     7561 gcctgttatc ctgttccgat gaagaagata ccgtttcaaa gctctgtgcc ctatccgccc
     7621 ccgcggcatt aaagcttatt atcacagccg ctgtaagcat gataaggata ccgattccta
     7681 tcagaatact ttttttgtgc cttagaaaca tttttttcac aatattacct ccaactcagc
     7741 tattccacgc tttttaacga ctcaatcata tcaatgcttt tcatttttac ggctgcgatg
     7801 ctgttgataa acaaagcaaa tactgctgtt atggccactg aaaaaaccac ggtggcgaag
     7861 gatacattgc cgatcaaatc catctggtca ttcaggccct ttgcaacaat ttcagccagc
     7921 accttgccca caggcatgcc cagaatcagc cctgctatta ctgaaagggt gttttcagtg
     7981 agtacaaggg aacggatttc cttctggtaa aaacccagga ccttcaaagt tgtaagatcc
     8041 cttatcctct cggaaaaatt caatatgctg ctgttataaa gaacaacaaa ggccaggatt
     8101 cctcccataa taataagcat taccccggct atataaacaa cccttgtgct ggatttgaca
     8161 tcggcaatct gatcggctct gcttacatat tcctccgcat aatctcccct gagaaagctc
     8221 caatccggcc cgttgtccca ttttacaagt attgcggtgg gcttaaaaat ttctccgatt
     8281 gattcatagt aggtatcgga catatacatt ccctggccgg tggccagata cacaatctgt
     8341 tttacaggca ctcttatata gcctttgttt gtccgcttca gttctattat atctcctgcc
     8401 ttgattttca gggtgtctgc aagctttctc gtaatggcgg ccccttcatc aggcagcagg
     8461 acaggattgc cttcgacatc cttcaggcgg ataagagggc ttttctgcgg agagaccgtc
     8521 agcaattcca attgcctttc tccattggga cagatgattt ccgctgctgt ttcccttatc
     8581 tgctgaaccg ttccctccag ccccatgtta tgtataaaac gggaatccgc tttactgtcc
     8641 aggaggattt tatggtcgta agtgtatgta acattgtaag tatgccgggc aattccatta
     8701 atcatattaa ttatggtaaa ggctgctaca atcagccccg tgcagcctgt gattcctagt
     8761 atgctcatta ttaggcgtat tttgtttctg gatgtattcc gggcaatcag cctgcggctg
     8821 aagcgcatcc tgctccaaag gccggacagg tattccagga atatatggct tccttccttg
     8881 gggggcttaa ttctcagaag cacggaaggg gaatctccca gcagcctgag gcaggaatac
     8941 agggaaatgc ctccggtgca aagaagtatc aaaaccaggc ttataataaa attactgtgg
     9001 tttattgaaa ttttgtaatc ggtaaagttc agcctgagcc aggggataag catcttgccg
     9061 aagataacgg ggcctgaaag gaggcctaaa aaagccccaa gcagccccat atatacccca
     9121 taggaggtat aatgccacag gatgcttttc tttccgtacc ccagagcttt taagatacct
     9181 atctggcccc gttggttttc caccagcctc atcatggtgc tttgggttat taaggcagtc
     9241 acaataaaaa acatcaggga gaataccgca gcaaggagct tgaactgctg gatattggca
     9301 ttgatattgc tgacgcttct gtggtcctct ctggcgacaa tacctgccag atcatctccc
     9361 acggcattaa aaatatgggt ttttaccttt gttatatcgg caccgggaga cagctttacg
     9421 gatatctggt tatatatttt ccgaccgtat gcattcttta gcatgtcttc attaataata
     9481 atgaagccgt attttttcgg atcagggaat gtgtcagtgg agcctttcac cgaataaata
     9541 tgttcactgc ttaaagccag gccctggata gggaagcgta tccacttatt attcagttta
     9601 atcgagatat catcacctat atccaatccg tgcattcttg caaaaacttc gtccaggatt
     9661 gccccgctgc ggcttgaaag ctttccgcta agaagctggg gccggtccag tacacctttg
     9721 ccggaaacag catatacacg cagggtaggg ctgttttcca gatctgataa ggcatccagc
     9781 acaaaacgct tttccgcctt ttccacccct tcaattcttg aaatgcccca aagctccttt
     9841 tcggtgggat tcaatacatt gacccataaa tccgatatgt tggcggctgc gtacatgctt
     9901 tccgaatgca gctccaccgt tttccagatg ctgtcgatac cggtgactat gcataccgca
     9961 agagtggcca ttataaggat agataaaaac tggactttcg acttttgcat atctctgagg
    10021 gatttgataa ataatgattg cattaccata ttatttcctc tgcagccttt gggctgctgt
    10081 tgtatctgat atcggaaatt tttccgtctt tcaaatgcaa gaccacatct gccatttccg
    10141 aaatcagtac attatgggtg acaataatca ccgtttttcc catatcgcgg gccgcatccc
    10201 gtatcagctt aaggactatc cgccccgttg cggaatcaag ggcccccgta ggctcgtcgc
    10261 ataataatat ctgagggttt tttgccaggg cccttgcaat agatactctc tgctgctccc
    10321 cgcctgatat ctggcttggg aaattgtcca tacggtcctt gagccctacc ttaatcagca
    10381 tttcttttgc gtccagggga tctttgcata tttcctccgc aagcagcaca ttttccagta
    10441 ccgttaaatt gggcaccagg ttatagaact gaaaaacaaa gccgatttta ttccttctgt
    10501 aggttgtaag ttcatattca gccattccgg atatctccag attgtccgca atgattcgtc
    10561 cttttgtcgg tgaatccatg cctccaagca aattgagcac tgttgttttg cctgcaccgc
    10621 tttgccctaa tactacattt acagtaccct tttctatgcc gaagctgacg ccgtcaaccg
    10681 ccttgatgat attgccgccc acatggtatt ccctttctac attttcaaaa gtaatgaagc
    10741 tcataggtta tctcccttta tgcatatatg ctgcagcaaa actcagatgc catctgctgc
    10801 ctcacttatt aaacaattat agtggaaata aattatatag taagaggaaa tttattaaga
    10861 aatattaaga atggagaaca ttcttcaggt gcgaggttgg aggtgcgggg tacggggtac
    10921 gagattttga gcggggcttt gaagaaaatg ctgttcaaaa actgagcaat aatacaaaaa
    10981 agtgtacaaa taatgaccag ttttctttaa ggttatagga tatccgttga gaatactggt
    11041 ttttcttttt ggcatgggaa ttgctataaa tagagg
//
LOCUS       DAOQUR010000069        11036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_6667_length_11036_cov_12.401056, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000069 DAOQUR010000000
VERSION     DAOQUR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6667_length_11036_cov_12.401056"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKI50_10795"
     rRNA            complement(<1..284)
                     /locus_tag="PKI50_10795"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(859..1509)
                     /locus_tag="PKI50_10800"
     CDS             complement(859..1509)
                     /locus_tag="PKI50_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNT04227.1"
                     /translation="MKMKVRDPFSGFSHLAGAILSVAGLYLLVRYALVNGTVWHIVSF
                     SIFGASLILLYTASSIYHLLSVSEKSTRVLRKIDHMMIYILIAGTYTPVCLIPLRGGW
                     GWSLLISIWGIAMAGIVLKVLWFNAPRWLYTLFYLLMGWLIVIAFVPLVRTMPIAAML
                     WLIAGGLLYTVGAVIYGTKWPKLKSKVFGFHEMFHIFVLYGSFCHFWMMFRYILYL"
     gene            1697..2290
                     /locus_tag="PKI50_10805"
     CDS             1697..2290
                     /locus_tag="PKI50_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HNT04228.1"
                     /translation="MDFNEDSFFKIVEDISQGRDIRIPDIPDIELYMDQVTTFIDNKL
                     GYNKRILKDKILTKTMINNYTKSKILLPSKNKRYNRQHVILLILIYYLKQILSINDIS
                     ILFVPLFKNITSSNDGAGYLENLYNAFLEVKDNNANDLKSILSQKLDLISTKAEEFPN
                     DSKETSKILLTVLMLVATAGIQKRIAEKIIDDYLTKK"
     gene            complement(2314..3108)
                     /locus_tag="PKI50_10810"
     CDS             complement(2314..3108)
                     /locus_tag="PKI50_10810"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNT04229.1"
                     /translation="MKWLDKLERRLGRFAIKNLMSYIVFMTGIVYALSYFDRSGFVIS
                     KLMLIPELVLRGEVWRLVTYIFIPPNTSIIWILFTLYFYYMVGSALEQEWGSFKFNIF
                     YFTGMIGTTIAAFITGYGATALYLNLSLFLAFAKIYPDFELLLFFVIPVKMKYLAMLN
                     WAFIIYTVLVGDLSAKLIAVVSIMNYFLFFGKDIIAGTKSSRQVYNNRRRFQSELPRD
                     FTIHKCTICGKTEKDDPKMDFRYCPQCEGDYEYCMDHVRNHEHVRE"
     gene            complement(3141..3965)
                     /locus_tag="PKI50_10815"
     CDS             complement(3141..3965)
                     /locus_tag="PKI50_10815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT04230.1"
                     /translation="MIRPLNEWDRAIILDYLSRNEDEAAFVFGNILHCGITNRKDVLR
                     CGDYYGYFEGDNLRGILVFYNLGTCIPHYESVGAVPFLSKLMIMGRFNVLLGKDQIVR
                     PLFEAIKNYKELKRFEECSYQINRNFKPFKLEGAVFTDVSGNDDTSIISFVRKAYWRG
                     FGAERTIEGTRILLLQKNEEEEFIILSVDNKLVAQACIQTFTDNTNQIGAVYSLEEER
                     GKGYAKAAVSELCERIIGRGKLPTLIVSKQNIPALNAYKALGFEHYSDYLIVKLFI"
     gene            complement(3995..4543)
                     /locus_tag="PKI50_10820"
     CDS             complement(3995..4543)
                     /locus_tag="PKI50_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04231.1"
                     /translation="MKQHITIENLMELNEYQRDRLNSLWNPVRYDLAAGFLCKDAENN
                     EYDIFPFVIGYVNIRETRSGYSMTLMNLEALRSWKVQEEEAQEETGAEEEFSEEDFVF
                     EYERPDIYNKEDCLPLLNIGQMIEILNKCAYGKGSFYINLRKDPKDCSIGRNIEEYID
                     YGTDYNGEELCGVLWTAIKEAL"
     gene            complement(4574..5329)
                     /gene="murI"
                     /locus_tag="PKI50_10825"
     CDS             complement(4574..5329)
                     /gene="murI"
                     /locus_tag="PKI50_10825"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNT04232.1"
                     /translation="MSIGIFDSGVGGLTVLKEAVKLLPGEDFLYYADTKHVPYGTKPK
                     DEVKGYIFHAADFFAEHKAEALVVACNTATSIAINDLRKKYSFPIIGMEPAVKPAVEK
                     ADDRRVLVFATPITVREKKLHDLVIRLDSEQTVDLHALPGLVELAERFIFDEETVTQY
                     LLDELSGYDLKEYGAVVLGCTHFVFYRNYFKRILPHGVEVIDGNTGTVNRLRALLESR
                     GLDYGRKGSLEFYRSGEKETDDSKYRRYLELDY"
     gene            complement(5632..6372)
                     /locus_tag="PKI50_10830"
     CDS             complement(5632..6372)
                     /locus_tag="PKI50_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT04233.1"
                     /translation="MIEKIYLFAVVLASFFVKGITGFGNTLVMAPLFSFVVSNRFTTP
                     VDLLISIPTNSYLVWKNRKSINYRTVVPLSIMLLTGVVPGIMLLKTGNDLLLKSILGV
                     VIMALGIEMLYRKPGHKGLAKPNKLFLMTVGLMSGVLAGMYGISAFLVAYISRTSEDR
                     SQFRGNLCSLFLVDNIFRLFWYSAAGIMTMEIVKFTLFILPAVALGMYIGTRVDTGLK
                     EETVRKATIYLLIVSGAALLLRSIYSTL"
     gene            complement(6385..7092)
                     /locus_tag="PKI50_10835"
     CDS             complement(6385..7092)
                     /locus_tag="PKI50_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNT04234.1"
                     /translation="MNLNEELYKLARSCMISYFGVADLSKVEHAAIDQAGTGIGDYPY
                     CVTMGIPLPKDIVDRLSYREHYNDALNYKLFAYDVINQRLDLAASMLASLIQEKGYRA
                     LPIPASGIIDHDRIYGAFSHKLGARLCGFGWIGKNCLLITSQNGPRVRWVSILTDSPL
                     QPTGEGIMEPRCGECTACADICPVNAIKGKTFTENESREARFDAHKCEAYFNSLTSAG
                     KLYVCGMCLYACPHGRK"
     gene            7234..8064
                     /locus_tag="PKI50_10840"
     CDS             7234..8064
                     /locus_tag="PKI50_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNT04235.1"
                     /translation="MVEYLSQFNLICLGIMASLGAGLATGIGSIPVFLTKKISKKLLD
                     LLLGTAAGIMLAATSFSLIIPAIEKGGGGIKGASTALVGILFGGVFIDFIDKFFPDTN
                     LLLNALNGNGNNAHEVNKSETAEINSRLRKVWLFVIAITVHNFPEGLAVGVGFGDGDI
                     MNGLCLAIGIGLQNLPEGLAVALPLLRENYPRGKAFLVALCTGLVEPIGGLLGIVLVQ
                     ISRPLLPFALAFAAGAMLFVISHEIIPETQHQKCHSKLATHALLVGFVVMMFLDNTLG
                     "
     gene            complement(8098..9006)
                     /locus_tag="PKI50_10845"
     CDS             complement(8098..9006)
                     /locus_tag="PKI50_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT04236.1"
                     /translation="MESWEKIKAVQRMQSYIEEHLAETVTLNMLGKAAGYSPWHSERI
                     FKELTGKTPFEYIRALRLSRAAVKLKDRNTRVIDVAFDFVFDSHEGFTRAFSKQFGVT
                     PKYYSKNTPEIKLFMPGRIRDYYLRLQKGEDNMCKNPNTNAVFVQVVDRPARKLIFKP
                     GVKATHYYEYCEEVGCDVWNVLSGIKEAIYEPIGMWLPDNLRRPGTSTYAQGVEVPAD
                     YTGKVPEGYELMDLPPCKMMVFQGEPFDEEKFEEAIGNLWEVMKKYNPELYGFKWADE
                     DGPRFQLTPMGYRGYIEARPVRQLNA"
     gene            complement(9183..10067)
                     /locus_tag="PKI50_10850"
     CDS             complement(9183..10067)
                     /locus_tag="PKI50_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT04237.1"
                     /translation="MTNKTKADLMLLAVTVFWGASYMLTKIALDVLEPFNLSALRFLI
                     AFVVSALVFYKNILKSDIRIIKYSLILAVILVGMFISMSFGLKYTTASNAGFLISLAV
                     IIIPILSYVFLKQKIEKRVLIGVCIAPIGIALLTLNSQLSVGSGDLLCILCAFLSALH
                     VVVMGVFTRKVDSIALGVLQLGFAGILSIVFSLFTETLRLPSTGISWGATLALSILCT
                     AFGYIVQATAQQYTSATHTGLILSLEPVFSAILGFAFLGEILSPRGYFGAAVLLLSVL
                     IAEVGFRKKDVKAKEIDV"
     gene            complement(10456..>11036)
                     /locus_tag="PKI50_10855"
     CDS             complement(10456..>11036)
                     /locus_tag="PKI50_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04238.1"
                     /translation="EKEDLPEFSKTLQKEEISQLMEWLTEKDDKLRYHTLLLLQYRSE
                     HYDDVYPFWDVFCEKLKSPNSYHRSLGLMLMADNVKWDRENKIDGAIDDYLKILYDEK
                     PIIVRQCIQALGKIVPYKTQLHEKIANELMAINIMDVKQTMRKLILLDILNILALIRK
                     NRTSEEIDGYISKALTGELLDKKSKKQIESML"
BASE COUNT         3138 a         2589 c         1954 g         3355 t
ORIGIN      
        1 atcccccact ccaaggcagg ttgcccacgt gttactcacc cgtccgccgc taagatttct
       61 ttggcactca atactctaat tcttcttccc tctctgtgct ggcactttaa aaacgcgtga
      121 tgaagaaact tttatcagtg tttttaacat ctatgttgtt tcttcatgac gcttcctttg
      181 cgtcactact agagtactca gtgccaaaga aatctccgct cgacttgcat gtgttaggca
      241 cgccgccagc gttcgtcctg agccaggatc aaactcttaa gttaatattt gattcgcttt
      301 cgcgaattct aactttttca tttgccctaa ctcaaaagaa ttctgactgt tttcagcgca
      361 gattgctctg cacttcaaac tttcatttgg ttatcatttt acactgttta gttttcaagg
      421 atcaatcctc atatattata tggaagatct tgcgattctt ctcatatcct gccggcgaat
      481 cagccgccga cctttactat gttatcacac cacttttctg ttgtcaatac tgatttcgct
      541 ggtttttcgt ggtaattcgc atccctcgca ttcgtaattt ggaacaacgt ttaagaatat
      601 atcatataag ttccagtgag taaagcacac cctgccgcta attttcacgg ttaatattca
      661 atattgttgg ttatctcctt aactctcatc atagctttac attatgtaaa cttataatcc
      721 gtaatttaaa tcatacgtaa ttatattttc ctgcgcacat tattatatgt aggaaaattt
      781 gaacagtcac atgttatttg ctcaccaata aaaatttgta cagaaaaaat gcgccccaag
      841 acgcattcga ttctattatt acaaatacaa tatgtatctg aacatcatcc agaagtgaca
      901 aaagcttccg tacaatacaa aaatatgaaa catctcatga aacccgaaaa cttttgattt
      961 cagcttcggc catttggtgc cgtaaataac tgcgccaaca gtgtacaata aacctccggc
     1021 aataagccaa agcattgccg ctatcggcat tgtcctgaca agcggtacaa atgcaattac
     1081 tataagccat cccatcaaca gataaaaaag ggtatacagc catctgggcg cattgaacca
     1141 cagtactttt aatacgattc cggccattgc aataccccaa atacttatga gaaggctcca
     1201 tccccagcct cccctcaatg gaataaggca taccggtgta tatgttcccg ctataagtat
     1261 ataaatcatc atatgatcta tctttctcag aaccctggtg cttttctcgg ataccgacag
     1321 caggtgatat atggaacttg ccgtatatag aagtatcagg cttgctccga atattgaaaa
     1381 ggatacaata tgccacactg tcccattcac caaagcataa cgtactaata agtaaagtcc
     1441 tgcaactgac agaatcgccc ctgcaaggtg tgaaaatccg ctgaaagggt ctcttacttt
     1501 cattttcata taatatctcc tcttctactt aatgcattcc ccatatagta tttactacta
     1561 tgtgttgtca tatttatatt atatattgta gtctttatat tttcaattca tatttctaat
     1621 ctttgtgctt catttttatg gtatactata tctcagaggt ttgtctgaat aaaactcata
     1681 gtgaggagat ggttaaatgg attttaatga agattccttc ttcaaaatag tggaggatat
     1741 atctcaggga agggatataa gaattccaga tattcctgac attgagcttt acatggatca
     1801 agtcactaca ttcattgata ataagctggg gtacaataaa agaattttaa aggacaagat
     1861 tctgacaaaa acaatgataa ataattatac caagtcaaaa atccttctgc cttccaaaaa
     1921 taaaaggtac aataggcagc atgtgatact ccttatactt atatattatt tgaagcaaat
     1981 tctgtcgatt aatgatataa gcatattgtt tgttcctttg tttaaaaata ttacttcctc
     2041 caatgacggg gcgggctatc ttgagaactt gtacaacgcc tttcttgaag taaaagataa
     2101 taatgcaaat gatctgaaaa gcattctttc tcaaaagctt gatctaatta gtaccaaggc
     2161 ggaggaattc cccaatgact caaaggaaac ctcaaaaatt ctgctaaccg ttctaatgct
     2221 ggtagcaaca gcaggtatcc agaaaagaat tgctgaaaaa ataattgacg attatctgac
     2281 aaaaaaatga ggccgtattt tggcctcatt ttcttattcc ctcacatgct catggtttct
     2341 cacatgatcc atgcagtatt cgtaatcccc ttcacattgg gggcagtatc tgaaatccat
     2401 tttggggtca tccttttcag tcttgccgca gatcgtacat ttatgaattg taaaatccct
     2461 gggcagctca gattggaatc ttctcctgtt gttgtacacc tgcctgctgc tttttgtccc
     2521 ggcaataata tccttcccaa agaacagaaa gtagttcatt atcgagacta ctgcaatgag
     2581 ttttgcggat aaatctccaa ccagtaccgt atagattata aaagcccaat tcagcatcgc
     2641 caggtatttc attttgaccg gtataacaaa aaacagaagc aattcaaaat caggatatat
     2701 tttcgcaaat gccagaaata atgataagtt cagatatagg gccgtggctc catatcccgt
     2761 tataaatgcc gcaatagtcg tacctatcat gcctgtaaaa tagaaaatat taaatttgaa
     2821 gcttccccat tcctgttcaa gtgcgcttcc gaccatataa tagaagtaca gcgtaaacag
     2881 aatccaaata attgatgtat taggcggtat gaatatataa gtcacaagcc tccatacctc
     2941 tcccctcaat accagctcag gtatgagcat cagcttgctt atgacaaacc cgcttctgtc
     3001 aaaatatgat aatgcgtata caatgcctgt cataaacaca atatatgaca tcaggttttt
     3061 aattgcaaat ctgcccaatc ttctctcaag tttatccagc catttcaaat gtatttcccc
     3121 cttaatccca caagtattaa tcatatgaaa agttttacaa tcagataatc gctgtaatgc
     3181 tcaaagccaa gagctttgta cgcattcaaa gccggaatgt tctgtttgct tacaataagc
     3241 gtcggaagct ttcccctgcc tattatcctc tcacacagct cagaaaccgc tgccttggca
     3301 tagccttttc ctctttcctc ttcaagggaa taaactgctc ctatctgatt cgtattatct
     3361 gtgaaggttt gtatgcaggc ctgcgccacc agtttattat ccacggataa tattataaac
     3421 tcttcctctt catttttttg aagaagtaat atcctcgtgc cctctatggt ccgctctgcc
     3481 ccgaagccgc gccaatatgc tttcctaaca aagcttatta tgcttgtatc atcatttcca
     3541 gatacatctg tgaacactgc tccttcaagc ttgaaaggct tgaaattcct gttgatctga
     3601 tagctgcatt cttcaaacct cttcaattcc ttgtaattct ttatagcttc gaaaagaggc
     3661 cttacgattt gatcttttcc caaaagcacg ttgaacctgc ccattatcat cagtttggaa
     3721 aggaatggta cagccccgac tgactcataa tgcggaatgc aggttcccag attgtaaaac
     3781 accaagatgc cccttaagtt gtcgccctcg aaatatccgt aataatcgcc gcagcgcagt
     3841 acatccttcc tattggtaat gccgcagtgc agaatatttc cgaaaacaaa agctgcttca
     3901 tcttcattcc tgctcaaata atccagtatt atagccctat cccactcatt taaaggcctt
     3961 atcaaaatac atcaccccta tatgctctta atttctacag agcctcttta attgctgtcc
     4021 atagaacacc acacaattct tcaccgttat aatccgtccc ataatctatg tactcttcaa
     4081 tattcctgcc gatgctgcaa tcctttgggt cttttcgcaa gttgatataa aaactccctt
     4141 tcccgtatgc gcatttgttc agtatctcta tcatttgccc tatgttaagg agaggcagac
     4201 aatcctcttt gttatatatg tcaggccttt catactcaaa tacaaagtct tcctcgctga
     4261 attcctcttc cgccccggtc tcttcctgag cttcttcctc ctgaactttc caacttctaa
     4321 gagcttcaag gttcatcagc gtcatggagt aaccggatct tgtctctctt atattcacat
     4381 agccaataac gaatggaaaa atatcgtact cattattttc tgcatccttg caaagaaatc
     4441 ccgccgccaa atcatatctg acgggattcc aaaggctatt gagcctgtcc ctctgatatt
     4501 cgttaagttc cataaggttt tcaatagtaa tatgctgttt cataagaatg cctcctcgta
     4561 ttggttttat tatttagtaa tcaagctcca gataccttct gtatttgctg tcatcggttt
     4621 ccttctctcc tgaacgatag aattccaaac ttccctttct gccataatcc aatcctctgc
     4681 tttccagcaa tgctcttaat cggtttacag tcccggtatt gccgtctatt acttcaactc
     4741 catgaggcag tatccttttg aagtaatttc ggtaaaatac aaaatgggtg cagccaagaa
     4801 cgacagctcc gtattctttc aaatcgtatc cggaaagctc atccagcaag tattgagtta
     4861 cagtctcttc atcaaatatg aacctctctg caagctctac cagtccggga agtgcatgca
     4921 aatcaaccgt ctgctctgaa tccagcctta taaccaagtc gtgcagcttc ttctccctga
     4981 cagtaatagg tgttgcaaac acaagcaccc ttctgtcatc agccttttct actgcaggct
     5041 ttactgccgg ctccatacca attatgggaa aactgtactt ctttctcagg tcatttatag
     5101 caatgcttgt agctgtattg catgccacaa ccagagcctc ggctttgtgc tccgcaaaaa
     5161 aatctgcagc atgaaaaata tatcctttga cttcatcctt tggttttgta ccataaggaa
     5221 catgcttagt gtcggcatag tataagaaat cttcaccggg aagcagcttc actgcctcct
     5281 tcaatactgt caggcctcct acgccggaat caaaaatacc tatactcata tatatctcct
     5341 caaatcttgc aaaattaaac tacacacttt atcatcagtt ggggtcatct ccactgatga
     5401 tatattatat gccttgtggt gaattttcat cagacaatat ttattaattt tccaataaat
     5461 tctaaataat atacaggttc aagtaccttg taacatctaa ttgttgcgtt atgatacgca
     5521 ggatatttgc ttccaataca aggcgatggg aagaggcgta gcagtggcta cggcgattga
     5581 catcaacgca gtagtgggac aaatagacaa gtatcatgca gcttttaggt gttacaaggt
     5641 actatatata cttctcaata gcagagccgc tccgctcaca ataagaaggt aaatagtcgc
     5701 tttcctcact gtctcttctt taagccccgt atccactctc gtacctatat acattcccaa
     5761 ggccactgcc ggcagtataa ataaagtgaa cttcacaatc tccatcgtca tgattcctgc
     5821 tgccgagtac caaaaaagcc tgaatatgtt gtcaacaagg aaaaggctgc acagattccc
     5881 tcggaactga ctcctgtcct cactggtcct gcttatgtat gcaacaagga aagcactgat
     5941 tccatacatg ccggccagta ctcccgacat cagccccacc gtcataagaa acagcttgtt
     6001 gggtttcgct aatcctttat gtcccggctt tcgatacagc atctcaatac caagtgccat
     6061 aataacgact cccagtattg attttaacag caggtcgttc cctgttttta gaagcattat
     6121 acccggtaca actccggtaa gcagcattat cgataggggc accacagttc tgtaattgat
     6181 gctcttcctg tttttccata caaggtatga attggtaggt atgctgataa gcaagtcaac
     6241 cggtgttgtg aatctgtttg atacgacaaa ggaaaacaaa ggtgccatta caagggtgtt
     6301 cccgaagccg gtgatacctt ttacaaaaaa ggaggcaaga actactgcaa acaaatatat
     6361 tttttcaatc aatgaacgct accttcactt tcttccatgg ggacatgcat atagacacat
     6421 tccgcacaca taaagctttc ctgctgatgt cagagagtta aaataagctt cgcatttatg
     6481 agcatcaaaa cgcgcttctc tggattcatt ttcggtaaaa gttttgcctt taattgcatt
     6541 tacagggcat atatcggcac aagccgtgca ttccccgcac cggggctcca taatcccttc
     6601 cccggtaggc tgaagaggcg aatcagtcag tatggatacc catcttaccc tgggcccgtt
     6661 ttgagatgta atcagcaggc agttctttcc tatccaaccg aagccgcaaa gccttgcacc
     6721 cagcttgtgg gagaatgcac catatatcct gtcatggtca ataattccgg atgccggtat
     6781 gggaagcgcc ctgtatccct tctcctgtat aaggctggca agcattgaag cagccaaatc
     6841 caatctctgg tttattacat catatgcaaa aagcttgtag ttcagtgcat cattgtaatg
     6901 ttccctgtat gacagtctgt ctactatatc tttcggtaaa gggatgccca ttgttacaca
     6961 gtaaggatag tctcctatac ctgttcctgc ctgatcgatg gccgcatgct caaccttgga
     7021 caaatccgcc actccaaaat atgatatcat acagcttctt gcaagcttat acagctcctc
     7081 attcagattc atgaatctgt tccctcccta tacgctcttc tcttaattat atataacaat
     7141 gcataataat atcaagctat atggagtacg gctgtaacaa tattattgct gaatcataat
     7201 atgaaatagc gggtattgta aggaggtcca tatatggttg aatatctctc acaattcaat
     7261 ctgatatgtc tgggaataat ggcaagcctg ggtgccggcc tggccacagg tatcggctcg
     7321 atacctgtat ttttaacaaa aaaaatatcc aagaagctgc ttgatttgct gttgggtaca
     7381 gctgccggaa taatgcttgc cgctacatcc ttcagtctga ttatccctgc tattgagaaa
     7441 ggcgggggag gaataaaggg cgcatcaaca gcccttgtcg gaatactgtt cggaggtgta
     7501 tttattgatt tcattgataa attctttccg gacaccaacc tgctcctgaa tgcgttaaac
     7561 ggcaacggta ataatgctca tgaggtaaat aaatctgaaa ctgcagaaat taattcacgg
     7621 ctccggaagg tatggctgtt tgtaattgca atcactgttc ataacttccc cgaaggcctt
     7681 gctgtgggcg tgggctttgg agacggcgat ataatgaatg ggctgtgtct tgcaatcgga
     7741 ataggcctgc agaacctgcc cgaaggcctt gccgtggccc ttcctttgct gcgggaaaac
     7801 tatccaagag gcaaggcttt tctggtggct ttgtgtacgg gtctggtaga gcccatcggc
     7861 ggattgctgg ggattgtcct tgttcaaata tccaggccgc tgctgccttt tgccctggcc
     7921 tttgctgcag gagccatgct gtttgtcata agccatgaaa taatacccga aacgcagcat
     7981 cagaaatgtc attcaaagct agccacccac gctcttttag tgggatttgt ggtaatgatg
     8041 tttctggata atactttagg ctgagggagt tctataaaga actccctcaa taattaatta
     8101 tgcattcaat tgcctgaccg gccttgcttc aatatatccc ctgtagccca tcggtgtaag
     8161 ctggaacctc ggtccgtcct catcagccca cttaaatccg taaagctccg ggttatactt
     8221 cttcataact tcccacaggt ttcctattgc ttcctcgaat ttctcctcat cgaagggctc
     8281 tccctggaat accatcatct tgcacggagg aaggtccatc agttcatagc cttccggcac
     8341 ttttcccgta tagtctgccg gcacctcaac cccctgtgcg taggttgacg tacctggcct
     8401 gcgcagatta tcaggcagcc acattcctat aggctcataa atagcctctt tgattccgga
     8461 cagtacattc catacatcgc agcccacttc ctcgcagtat tcataataat gagtcgcctt
     8521 gactccgggc ttgaatatga gctttcttgc cggtcggtcc accacttgaa cgaaaacagc
     8581 attggtattt ggatttttac acatattatc ctctcctttt tgtaatctga ggtaataatc
     8641 acggatgcgc ccgggcataa aaagcttgat ttccggcgta ttcttgctgt agtatttcgg
     8701 agtcacgcca aactgcttgg aaaaagctct ggtaaatcct tcgtgggagt caaatacaaa
     8761 atcaaaagcc acatcaatta cccgtgtatt tctatccttc agcttaacag ccgctcgaga
     8821 caacctcagc gcccggatgt actcaaaagg tgtcttaccg gtcaactcct tgaaaatcct
     8881 ttcagagtgc cagggtgaat atcctgcagc tttccccagc atattcagag taactgtctc
     8941 tgccagatgc tcttcgatgt aactttgcat tcgctgaacc gcctttatct tttcccagct
     9001 ttccatgatt tcacctccta gatatttcgc gaaaaacatt ttacacatat aattcagatt
     9061 tcgcgtaaat atttccttga tctcattgaa ttcgcgctat accctgatga aataccttaa
     9121 gcgcgttcaa tataagtaga ggatatcaat tataattagt tatttcttga cttgttttgc
     9181 tgtcatacat ctatttcctt cgcttttaca tcctttttcc taaagcccac ctctgcaatc
     9241 aagacgctga gcaaaagaac cgccgcaccg aaatatcctc ttggagacag tatctctcca
     9301 aggaaagcaa aacctaaaat tgcggaaaaa accggctcca gggatagaat aagccctgta
     9361 tgggttgcac tggtgtactg ctgtgctgta gcctgcacta tatatccgaa tgcggtgcac
     9421 agaatgctga gggccaatgt agcaccccag gatatgcctg tgctggggag cctcaatgtc
     9481 tcggtaaata atgaaaacac tatactgagt atgcctgcaa aaccaagctg cagaacaccc
     9541 agggcaattg agtcaacttt tcttgtgaaa actcccatta ccacaacgtg caaagcggaa
     9601 agaaatgcac acaatataca gagcaagtct ccgctgccta cgcttaactg tgaattcaat
     9661 gtcaataaag ctatgcctat aggagctatg catactccta tcaacactct cttctcaatc
     9721 ttctgcttca gaaatacata tgaaagtatt ggtatgatga ttactgcaag gcttatcagg
     9781 aagcctgcat tggaagcagt ggtatatttc aagccgaagg acattgatat gaacatcccg
     9841 accagtatta ccgccagtat caaagaatat ttaattattc ttatatccga cttcaaaata
     9901 ttcttataga atacaagggc agatacaaca aaagcaatca gaaagcgcaa tgccgacaga
     9961 ttaaagggct caagtacatc aagagcaatc tttgtaagca tatatgatgc tccccagaaa
    10021 actgtaactg caagcagcat caagtccgct tttgtcttgt tagtcaaagt aatctcctcc
    10081 caagcaaaaa gaccatggca ttccatgatc ttaaaatatt gcgtcacgga tgcattggcc
    10141 cgtgactgta atatgaaaca ggcactttat ttacaatcct gggccgtaaa gtactatttt
    10201 tgattttgct cttattaata tagatttcat tcttacggcc tcccgattct tccagctttt
    10261 cttcactatt aatataacat ggtacggtat aggaagcaat acaaaattta ccaactgcca
    10321 ttgataatat gccagtataa gacctccaag gactgactat ggaatattaa taaacgtggc
    10381 ttctatagtc caatcctcaa tatcaaatga aggtaacatt tttaccatgc ttcatttgaa
    10441 tttcactcca ataatctaca acatcgattc tatttgtttt tttgatttct tgtcaagcaa
    10501 ttcacctgta agcgctttgg atatatagcc gtcaatttcc tcagaagtcc tgttttttct
    10561 gattaatgca aggatattaa gaatatcaag caatataagc tttctcatag tctgtttaac
    10621 atccataata tttatagcca taagctcatt agcgattttt tcatgcaatt gggttttata
    10681 aggaacaatt ttacctaatg cctgaataca ctggcgtact ataataggct tttcatcata
    10741 taagatctta agatagtcat ctattgcccc gtcaatttta ttctccctgt cccatttcac
    10801 attatcagcc ataagcatta gccctaaact tctatgatat gaattggggc tttttaattt
    10861 ctcacagaac acatcccaga aaggatatac atcatcatag tgttcagaac ggtattgcaa
    10921 cagaagtaat gtgtggtatc tcagcttatc atccttttcg gtcagccatt ccatcagctg
    10981 tgatatttct tccttctgca atgttttgga gaattccggc aggtcttctt tttcta
//
LOCUS       DAOQUR010000070        10960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_6721_length_10960_cov_11.230720, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000070 DAOQUR010000000
VERSION     DAOQUR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_10960_cov_11.230720"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..461)
                     /gene="cdd"
                     /locus_tag="PKI50_10860"
     CDS             complement(81..461)
                     /gene="cdd"
                     /locus_tag="PKI50_10860"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNT04239.1"
                     /translation="MNYDVLIEKAQEAMENAYVKYSNFKVGAALLTRSGKVYTGCNVE
                     NSSFGATICAERVAFTKAISEGEREFEAIAVASSLKGFTYPCGICRQFMSEWGLDIKL
                     ITKSGDEVRVHTIGELLPEAFVEF"
     gene            complement(467..1525)
                     /locus_tag="PKI50_10865"
     CDS             complement(467..1525)
                     /locus_tag="PKI50_10865"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNT04240.1"
                     /translation="MKNNRCKFMLMILCIIAVIFSGCGKATEPVSSTEPEVTPEPVVE
                     PSPPPAPEPPKGSPCPLTGELVTYEADLKLRPIAVMIDNEYNARPQSGLSEAEIVYEI
                     PVEGNITRYMAIFHHTHADKVGPVRSARPYFIDKALEFNAVYVHCGGSPQALQDLVTL
                     KVNTLNDLKGSPCFWRSKDRKMPHNLYTSTKLMREVMETNKFNNKTAPEYFKFNEEFL
                     EPDGKATRSIIFNYSKNYVVGYEYSEKDKLYYRTINGTRLKDKESGKDIAAANIIVQK
                     TTRKVLDDVGRLEIKNIGKGRGYYLSGGKLIEIEWNKDGRSAKEVYKDLKGNEVLMNK
                     GVTWVQVVPDYVKVEIRE"
     gene            complement(1563..2045)
                     /gene="ybeY"
                     /locus_tag="PKI50_10870"
     CDS             complement(1563..2045)
                     /gene="ybeY"
                     /locus_tag="PKI50_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNT04241.1"
                     /translation="MSVLIDNRQEEIKVDEALEAFVVQVVEKVLAYEQCEEAFEVSIS
                     FVNNQEMRKLNKEYRNIDKETDVLSFPLAEFIEEELNTEDEGAEYIEEEIALGDIVIS
                     MEKAAEQSEEYGHSFKRELAFLLVHGMLHLLGYDHDEEASEGEIFDKQEEILKNMEIA
                     "
     gene            complement(2151..4217)
                     /locus_tag="PKI50_10875"
     CDS             complement(2151..4217)
                     /locus_tag="PKI50_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNT04242.1"
                     /translation="MIKYSVSDLKRLARNSTTIRALLVAGLFIALFLLFVLNTAPQKH
                     LLQVGDISDKDIYAPTDIVDKSATELRRKQAEDSVQPVYNLDLTVQIDIEKKISDFFM
                     LLNSVRSNAELDKVQKLNLLKQNSNISLGNSLYNMLLDVSEADFASLEHNIKYINNQM
                     LSGRITEQQLNDKRSDIREFFMNLPKKSQLNEIGADIAVMVLKPNMYYDEQTTLARKQ
                     SEREKVEDVVIRKGTLIVGKGQELSSQQIQLLNAYGLLAESDKDRFDIQFYLGYGVII
                     GICLIIMGLYLRSLDKKLWDSNSTLLLISLTAIMALTVTMMLKNISQYLIVLPAGAML
                     VSILVSPRIAIMTNIMTSILAGFLGGFDLHVVVMMLLTGILGAGMVSKTHHRSTFILS
                     GLAVSLVSLLVIFSEGILNREDIYRMINNGLYGVIGGTLSAVLTIGTLPFFETVFDII
                     TPLKLLELSNPNQPMLRRLLIEAPGTYYHSILVGNLAEAAAEDIGANALLCRVGAYYH
                     DIGKLKRPYFFKENQITRDNPHDKITPNLSALVITSHVKDGVEIAGKNKLPSTVIRII
                     EEHHGNTLVAYFYHKALSAEGAEAVDESKFRYPFRKPQTEEAAIVMLADSVEAYIRSL
                     SEPTRDQVEQGVRKIIKDKLNDAQLDNCDLTLKDLEMIGQAFVKVLAGIFHDRIEYPE
                     TIKDVQ"
     gene            complement(4214..5149)
                     /locus_tag="PKI50_10880"
     CDS             complement(4214..5149)
                     /locus_tag="PKI50_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT04243.1"
                     /translation="MERIMNLFGNFDQNLKVIEDEFNIDIIVREGEVKIVGQENAVAM
                     AERTLNTLMKIQENGEAVTSHAVSYTIELVKEGRENRSEEFDSVIITNARGKAIKSKT
                     IGQKRYIDAIRNNEVVFGIGPAGTGKTYLAMAMAVKAFKNHEVSRIILTRPAVEAGEK
                     LGFLPGDLQNKVDPYLRPLYDALHDIMGAETYQKHIERGVIEVAPLAYMRGRTLDDSY
                     IILDEAQNTSPEQMKMFLTRLGFGSKAIITGDITQIDLPKGKYSGLKQVMEILKGIKG
                     IGFVYFSEKDVVRHKLVKDIIKAYEKHEEEIGESE"
     gene            complement(5278..6456)
                     /gene="yqfD"
                     /locus_tag="PKI50_10885"
     CDS             complement(5278..6456)
                     /gene="yqfD"
                     /locus_tag="PKI50_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HNT04244.1"
                     /translation="MFIIRIWNYLRGYAIIIVKGLKIERFINLAVVNNIYIWDIEKLD
                     YTTVKAKIRLENFGRLRDIVRKTDSSVSILNKRGFPFILRNIRRRKLFYSGLFLLCVF
                     IYIMSSFVWMIEIVGTKDVDNESILRCLYNEGLKEGSWKGRLDRRHIENQVLIKMPEL
                     SWIGIQIKGTKAVVEVVEKSEGPPLISRTDACDIVAAKDGVITKLLVLSGDPLVKDGD
                     SVRKGQKLVTGTILRENLEPRQIHSLAQVSARTWYEDAEEIPLQQIEFKPTGRVARQY
                     KLKLLDKEFTRKRSVNFKDFNEYVEERHLISFGDYVFPIKLIEYRFEELVPELKTIMV
                     DEAKERCAERLNARIKLRIPEDAVILKRDINYYTDKKSVKAKISVEVLEDIGVKQKIY
                     "
     gene            complement(6489..6761)
                     /gene="yqfC"
                     /locus_tag="PKI50_10890"
     CDS             complement(6489..6761)
                     /gene="yqfC"
                     /locus_tag="PKI50_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HNT04245.1"
                     /translation="MGKRSYKLREKLSNTFELPKDIVLDVSKIIIIGTGQITVENHKG
                     IIEYSEELIRINTGSGIVALHGKNLAIKTIFQEEITITGEITNMEF"
     gene            complement(6910..7410)
                     /locus_tag="PKI50_10895"
     CDS             complement(6910..7410)
                     /locus_tag="PKI50_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04246.1"
                     /translation="MEGIYIVVAIVIALFNLAAKQQKNQGRGRRGMNRQQGQYRQKPV
                     QRQKHIPQWSEGPFADLGKSWNELFGSPEEHVPESKPAEDYEGMEIEDRRRVGSLDYV
                     EHSSSSEGICDEHPEHSRLDALKAEIRETEEEGFIFEIAEDNLLRSVVMAEVLGPPRA
                     MKRRIR"
     gene            complement(7445..8437)
                     /gene="floA"
                     /locus_tag="PKI50_10900"
     CDS             complement(7445..8437)
                     /gene="floA"
                     /locus_tag="PKI50_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052352.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNT04247.1"
                     /translation="MSDLLFYVVSLFVIILGLTIFLSFVPLGLWISSLAAGVNVGIFN
                     LVGMRLRRVIPSRIVNPLIKARKAGLDVGVNQLEAHYLAGGNVDRVVNALIAAQRAEI
                     ELGFERAAAIDLAGRDVLQAVQMSVNPKVIETPVVAAVAKDGIEVRAKARVTVRANID
                     RLVGGAGEETIIARVGEGIVTTVGSSESHKSVLENPDLISRTVLAKGLDSGTAFEILS
                     IDIADVDVGRNIGAQLQTDQAEADKRIAQAKAEERRAMAVAKEQEMKAAVQEMRAKVV
                     ESEAEVPKAFAEALRNGRIGVMDYYQLQNVQADTQMRDAISKVGKSDSIEKEKK"
     gene            complement(8460..8942)
                     /locus_tag="PKI50_10905"
     CDS             complement(8460..8942)
                     /locus_tag="PKI50_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNT04248.1"
                     /translation="MFGDWGLLTAFLYVTGMVLLMIEALMPGFGVAGISGIVLVLASI
                     VMISSSFFQAILLLVGTTAIAVILVVALYRMGYGKRFVKSMILNTEQKNEEGYVSTKG
                     YEKYLGMRGTVATPLRSAGTVIIDGNKIDAVSEAEFIDKGIEVEVIKIEGGRIVVKRI
                     "
     gene            complement(9168..10847)
                     /locus_tag="PKI50_10910"
     CDS             complement(9168..10847)
                     /locus_tag="PKI50_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNT04249.1"
                     /translation="MNENTNESSNFIHDFIDIDLEEKVYDHVHTRFPPEPNGYLHIGH
                     CKAMYINFSTAEKYRGLCNLRFDDTNPIKEDVEYVQSIMEDIKWLGFDWEDRLYYASD
                     YFEKLYEFAEHLIKKGKAYVCDLSQQEVREYRGTPTEPGKNSPFRDRTVEENLDLFRR
                     MRAGEFPEGSRTLRAKIDMASPNLNMRDPVIYRILKSSHHRTGDKWCIYPMYDFQHPL
                     SDALEGITHSLCSLEYEDHRPLYNWVINECEPEHKPRQIEFARLNLTYTVMSKRYLRE
                     LVETGIVSGWDDPRMPTLCGLRRRGYTADALRDFCDRIGVAKTNSTVDYALLEHCIRE
                     DLNKKADRVMAVLRPLKVIIDNYPDNQIEYFEVENNPENPDSGTRKVPFLREIYIEQE
                     DFMENPPRKYFRLYPGGEVRLKGAYFIKCESVVKDPLTGDIKEIHCTYDSASKGGNSP
                     DGRKVKGTIHWISAEHAIDAEVRLYDQLFAVPNPGEEGHEYKDDLNTDSLEVLTGCKV
                     EPVLREASAGKGYQFLRMGYFCSDNKDFTKERPVFNRIVGLKDSWAKIVKE"
BASE COUNT         2655 a         2883 c         1942 g         3480 t
ORIGIN      
        1 gcaatcgcgc agcgattcca tgaaacagcc gtgtctcccg tgttacccgt tacctgcgtc
       61 gcattatcct actaatgcgc ttagaattca acaaaagcct cgggtaacaa ctccccgatg
      121 gtatgaaccc ttacttcatc tccgctcttt gtaattagct ttatgtccaa accccattcg
      181 gacatgaact gcctgcaaat tccgcaggga tacgtgaagc ccttcaggct gctggcaacc
      241 gcaatggctt caaactccct ctctccttcg gatatagcct ttgtaaatgc aactctttcc
      301 gcacatatgg ttgcaccgaa ggatgagttt tctacattgc agccggtata taccttgccg
      361 ctcctggtca gcagcgccgc cccgaccttg aaattcgaat atttcacata agcattttcc
      421 atggcttcct gtgctttttc aattaacaca tcgtaattca tgcctatcac tccctgattt
      481 caaccttcac atagtccgga accacctgta cccatgtaac tcccttgttc ataagcactt
      541 cattgccctt aaggtccttg tatacctcct ttgcgctcct gccgtccttg ttccattcaa
      601 tttctatcag cttccctccg ctgaggtagt atcctctgcc cttgccgata ttctttattt
      661 ccaaccgtcc tacatcgtca agaacctttc tggtggtttt ttgaacaatt atattggctg
      721 cagctatgtc ctttcccgat tccttgtctt tgagccttgt tccgtttatc gtcctataat
      781 ataatttgtc cttttcacta tattcatacc cgacaacata attcttgctg taattaaata
      841 tgattgatcg ggtagccttt ccatccggtt caaggaattc ttcgttgaac ttgaaatact
      901 caggtgccgt tttgttattg aatttattgg tttccattac ttctctcata agcttggtgc
      961 ttgtatacag gttatggggc atcttcctgt cctttgacct ccagaagcat ggggaccctt
     1021 tcaagtcatt gagggtattt acttttagcg tgaccaggtc ctgaagggcc tgggggctcc
     1081 cgccgcagtg cacgtatact gcattgaatt ccagggcttt atctatgaaa taaggcctcg
     1141 cacttctcac cgggccgact ttgtctgcat gagtgtggtg aaaaattgcc atatatctcg
     1201 tgatattgcc ttcaacaggg atttcataca ctatctccgc ctctgaaagc cctgactggg
     1261 gtcttgcgtt atattcattg tcaatcatca cagctatagg cctgagcttc aaatcagcct
     1321 catatgttac caactccccc gtcaatgggc aaggcgaccc cttgggcggt tcgggagccg
     1381 gaggcggact cggctcgaca accggttcag gtgtgacctc cggttcagta cttgatacag
     1441 gttcagtggc tttaccgcat ccggaaaata ttactgcgat aatacataaa atcatcagca
     1501 taaatttaca tctgttattt ttcatatgag accctctttc attaaataaa atcacaatat
     1561 ttctaagcaa tctccatatt cttcagtatt tcttcctgct tatcgaatat ttctccctcc
     1621 gaagcttctt catcatggtc atagcccaga aggtgcagca tcccatgtac cagcaggaaa
     1681 gccagttccc gcttaaagct gtgcccgtac tcctccgact gctccgctgc tttttccatt
     1741 gatatgacta tatcccccaa tgcaatttcc tcttctatat attcggcacc ttcatcctct
     1801 gtattaagct cttcttcgat gaactccgct aacggaaagg ataatacatc cgtttccttg
     1861 tcaatattcc tgtattcttt gttaagcttc ctcatctctt gattgttcac gaaagagata
     1921 ctaacctcga atgcctcttc gcactgctca taggcaagaa ccttttcaac tacttgtacg
     1981 acaaatgctt ccaaagcctc atcaactttt atttcttcct gtctgttgtc tatcaatacc
     2041 gacatataat tacctccaaa tattatttac ttcgtacttc aaagcccgcc ctaattcccg
     2101 tatctcgtat ctcgcaccct gtaccccgga cctcgcgacc ccgtaacaca ttattgtaca
     2161 tctttaatag tttccggata ttctattcta tcatggaata ttcctgccag caccttcacg
     2221 aaagcctgtc ctatcatctc cagatccttt agcgtaaggt cacagttgtc cagttgtgca
     2281 tcattgagct tatccttaat tatttttctc actccctgtt caacctgatc ccgggtaggc
     2341 tcggataatg acctgatata ggcctcaacc gaatctgcca gcataactat cgcagcttct
     2401 tccgtctgag gctttctgaa tggatatctg aacttactct catccactgc ttcggcccct
     2461 tctgcactta atgccttgtg atagaaatat gctaccagag tgtttccatg atgctcctcg
     2521 ataatcctta ttactgtgga ggggagcttg ttctttccgg caatctccac tccgtccttt
     2581 acatgggaag taatgaccag ggcacttagg ttgggagtaa ttttatcatg ggggttatcc
     2641 ctggtaatct ggttttcctt gaagaaataa ggccttttga gttttccgat gtcatgatag
     2701 tatgccccta ccctgcataa aagcgcattg gcacctatgt cttccgccgc cgcctccgcc
     2761 aggtttccca ccaatatcga gtggtagtag gtgccgggtg cttcgataag cagcctcctc
     2821 agcatcggct gattcgggtt tgacaattcc agaagcttta aaggggtgat gatatcaaat
     2881 accgtttcaa aaaacggcag cgtaccaatg gtaaggactg ctgaaagagt acctcctatg
     2941 acaccgtaca acccgttgtt aatcatcctg tatatatctt ccctgttcag aatgccttca
     3001 ctgaatatga ccagcaggct gaccaggctt acggccaatc ctgacaggat aaaggtactc
     3061 ctgtggtgtg tttttgatac cattcccgcg ccaagtatac cggtcaggag catcatgacc
     3121 actacatgca agtcaaagcc tccaagaaag cctgcaagta tggacgtcat tatattcgtc
     3181 attatggcta ttcttgggct taccaatatc gagacaagca ttgcccctgc gggcagtact
     3241 atgagatact gggaaatatt tttgagcatc atggtaacag tcaatgccat aatggctgtc
     3301 aagctgataa gaagtaaagt agaattgctg tcccaaagct ttttgtcaag gcttcgcagg
     3361 tacaggccca taataataag acaaatgccg attattaccc catagcctaa atagaattga
     3421 atgtcaaatc tgtccttatc actttcggcc agaagcccat aggcattgag cagctgtatc
     3481 tgctgagatg ataattcctg ccctttgccg actatcagag tacccttgcg gatgactaca
     3541 tcttccacct tttcccgctc gctctgtttt cttgccaatg ttgtctgctc atcatagtac
     3601 atatttggtt tcagaaccat tacggcaata tccgcgccaa tttcattaag ctggcttttc
     3661 ttgggaagat tcataaagaa ttcccttata tcgcttctct tgtcatttag ctgctgttcg
     3721 gtaatcctcc cgctaagcat ctggttgttt atatatttga tattgtgttc caaagatgcg
     3781 aaatctgctt ccgaaacatc aagaagcata ttatacaagc tgtttccaag gcttatgttg
     3841 gaattttgtt taagaaggtt aagcttctgc accttatcca gctccgcatt actcctgact
     3901 gaattcaaaa gcataaaaaa gtcgcttatc ttcttttcta tatcgatctg aacagtcaaa
     3961 tccaggttgt aaaccggttg gacactatcc tccgcttgtt ttctccttag ctcggtggcg
     4021 gacttgtcca caatatctgt tggagcatat atgtctttat cggatatatc acctacctgg
     4081 agaagatgtt tctgcggcgc agtattcaga acaaacagta aaaacagcgc aataaaaagg
     4141 cctgcaacca gtaatgctct gattgtagtg ctgtttcttg ccagcctttt taaatccgaa
     4201 accgaatact ttatcattcg ctctctccta tttcctcttc atgcttttca taagctttta
     4261 tgatatcctt taccagctta tgcctgacaa catccttttc actgaagtat acaaatccta
     4321 tacccttaat acctttcaat atctccatga cttgcttcag tccggaatat ttccctttgg
     4381 gcaggtcaat ctgtgtaatg tccccggtaa taatggcttt ggaaccaaag cctaatctgg
     4441 tcaggaacat tttcatctgt tcaggggaag tattttgcgc ttcatccagt atgatatatg
     4501 agtcatcaag agtccgtcct ctcatatatg caaggggtgc tacctctatt acccctctct
     4561 ctatatgctt ttgataggtc tccgcaccca ttatgtcatg caaagcatca tataaaggcc
     4621 ttagatacgg atctaccttg ttctgcaggt ctccgggcaa aaaacccagc ttttctccgg
     4681 cttccactgc aggccttgta aggattatcc tgcttacctc atggtttttg aatgccttta
     4741 ctgccatagc cattgcaaga taagtcttcc ctgtccccgc agggcctatt ccgaatacaa
     4801 cctcattgtt gcgtattgca tctatatacc tcttctgtcc tattgtcttg ctctttattg
     4861 ctttccccct ggcgtttgtg attataacac tgtcaaactc ctcactcctg ttttccctgc
     4921 cctcttttac aagttcaata gtatagctta ccgcatggct tgtaacagct tcaccgttct
     4981 cctgtatttt cataagagta ttcagagttc tttccgccat cgcaacggcg ttttcttgtc
     5041 ccacaatctt tacttcgccc tctctgacaa ttatgtcaat attgaactca tcttctatga
     5101 cttttagatt ctggtcaaag ttcccaaaga gattcattat tctttccata ttatcaattt
     5161 gtatactagc ttcagtaaat tgctcctgca attgttctcc tccttatgtg cttcggacac
     5221 catgcatgtg gtgcggacac catgcatgtg gtgcggacac catgtatggt gtccctacta
     5281 gtaaattttt tgtttcacgc caatatcctc aagcacctcg acacttattt ttgccttgac
     5341 agacttttta tctgtgtaat agtttatatc tcttttcaga attacggcat cttccggtat
     5401 ccgaagcttt attcttgcat taagcctttc cgcgcatctc tccttggctt cgtcaaccat
     5461 aattgtcttt agttcgggta ctaattcttc aaatctgtat tcaataagct ttattggaaa
     5521 tacataatct ccgaagctta tcaggtgcct ctcttccaca tattcgttaa aatccttgaa
     5581 gttcacgctt ctcttccttg tgaattcttt gtccagcagc ttcagtttat actgccgtgc
     5641 aacccttccg gttggcttga attctatttg ctgaaggggt atttcctctg cgtcctcata
     5701 ccatgtcctc gcacttactt gagcaagaga atgtatttgc ctgggttcca ggttttctct
     5761 caatattgtg cctgtgacca gcttctgccc ctttctcaca gaatccccgt ccttaaccag
     5821 gggatcgccg gaaagcacca gcagctttgt tatcactccg tcttttgccg caacaatgtc
     5881 gcaggcatca gtgcggctga tcagtggagg cccttcactt ttctcaacaa cctctaccac
     5941 tgccttggta ccttttattt gtatcccgat ccatgaaagc tccggcattt ttatcagcac
     6001 ctgattctca atatgccttc tgtccagcct tcctttccag cttccttcct taagcccttc
     6061 gttataaagg catcttaata tgctctcatt atcgacatcc ttggttccaa ctatttcaat
     6121 catccagaca aaggaggaca ttatgtaaat aaatacacac agcaggaaca ggccggaata
     6181 gaatagcttt cttcttctta tattccttag gataaaagga aagccccgct tgttcaatat
     6241 gctgactgaa ctgtccgttt ttcgtacaat gtccctgagc cttccgaaat tctccagcct
     6301 tatttttgcc ttgaccgttg tatagtcaag cttctcgata tcccatatgt aaatattatt
     6361 gactactgca agattaataa acctttcaat cttcagtcct ttaacaataa taatagcata
     6421 tcccctcaaa taattccata ttcgtatgat aaacatgcct cttatacccc cagcaacttc
     6481 aatgatactt agaattccat attcgttatt tctcccgtta tcgtaatttc ctcctgaaat
     6541 attgttttta ttgcaaggtt tttaccgtga agggcgacta taccgctgcc ggtgttgatt
     6601 ctaataagct cttcgctgta ttcgattata cccttatggt tttccaccgt tatctgcccg
     6661 gtaccaatga tgattatttt ggaaacatcc aggacaatat ccttgggaag ttcgaaagta
     6721 ttggacagct tctctcttaa tttgtaactc cttttaccca tagcacacac ctctgtataa
     6781 atgtatgcga aagaggtact ttgaagaaca aaaataggat tgccgcacaa taggacatgg
     6841 ggacgtttct cctgtcccgc tttcagcggg acaggagaaa cgtccccatg tcctattcct
     6901 ttttgacgct tatcttattc ttcttttcat tgctctgggg ggccccagaa cctcagccat
     6961 gactacgctt ctaagaaggt tatcctctgc tatttcaaat atgaagcctt cttcctccgt
     7021 ttctcttatc tcagccttta aggcatccaa cctgctgtgt tcagggtgtt catcgcagat
     7081 accctcggag ctgctgctat gctctacata atccaagctg ccgacccttc ttctgtcttc
     7141 aatctccatg ccttcatagt cttcagcagg ctttgattcc ggcacatgtt cctccggact
     7201 tccgaataac tcattccagc tttttcccag atctgcgaaa ggcccttcag accactgggg
     7261 aatatgcttt tgtctttgaa ccggcttctg ccggtattgg ccttgctgcc ggttcatgcc
     7321 tcttctgccc ctgccctggt tcttctgctg ctttgcagca aggttaaaca aggctattac
     7381 tatagctact actatatata taccttccaa ctacatccct cctttgatgg agatgctata
     7441 ggggctattt cttctccttt tcaatgctgt cactctttcc aacctttgat attgcatccc
     7501 tcatctgagt atcagcctgc acattctgca gttggtagta atccataacg ccaattctcc
     7561 catttctcaa cgcctcagca aatgcctttg gcacctcggc ttccgattct acaaccttgg
     7621 ctctcatttc ctggactgcc gccttcatct cctgctcttt ggcaacagcc atagcacgtc
     7681 tttcctccgc cttcgcctga gctatccttt tatctgcttc cgcctggtct gtctgcaact
     7741 gggcgccgat attcctgcct acatctacgt cggctatatc tattgaaagt atttcaaagg
     7801 ctgtaccgga atcaagcccc tttgccagaa ccgtccttga tatcagatcc gggttttcca
     7861 acactgattt gtggctctcg gagcttccga cggttgtaac gataccttca ccaactctgg
     7921 caatgatagt ctcttcgccg gcacctccta ccagtctgtc gatattggct ctcacagtca
     7981 ctcttgcttt tgcccttact tctataccgt ccttagctac tgctgctacc acaggagtct
     8041 caattacctt cggattaacg ctcatctgca cagcctgcaa aacatctctc cccgccaagt
     8101 caatagctgc cgccctttcg aagcccagtt ctatttctgc tctctgtgca gctataagtg
     8161 catttaccac cctgtcaaca ttacctcccg caagatagtg ggcttcaagc tggtttacac
     8221 caacgtcaag gccggccttt ctggccttta taagagggtt cacaatcctg cttggaataa
     8281 ccctcctgag cctcatgccc acaagattga aaatcccgac atttactcct gccgccaggg
     8341 aagaaatcca taatcccagg ggaacaaaac ttaaaaatat tgtcaagcca agtattatta
     8401 caaacaggga tactacataa aataataagt cactcatcga tatacctccg attctttaat
     8461 catattctct ttaccactat tctgccgcct tcaatcttta taacctcaac ctcaataccc
     8521 ttgtcaataa actctgcttc ggacactgca tcaatcttgt ttccgtcaat tatcacggta
     8581 cctgcagatc ttaacggagt agcaacagtc cctcgcatgc ccaaatattt ttcataaccc
     8641 tttgtactta catatccttc ttcatttttc tgttcagtat tcaatatcat tgattttacg
     8701 aagcgtttcc cgtaacccat cctgtatagg gcaacaacca gtataacagc tattgctgtg
     8761 gttcctacaa gcaaaagtat ggcttgaaag aagctggatg aaatcattac tatactggcc
     8821 aatacaagta caataccgct tattcctgca acaccaaacc ccggcataag tgcctcaatc
     8881 attagaagca ccatacccgt aacgtatagg aatgccgtaa gcaggcccca atctccaaac
     8941 atccgtttca cccccaacat tcaaatatta tatttatcca gtatattgta taaaatatag
     9001 aattatttat gccctgtatt atttaagtat aatattatca taacagtttt atgcagggtt
     9061 aacaactgga aataagttaa gtggggacac ggggacgttt ctcctgtccc gctttcagcg
     9121 ggacaggaga aacgtccccg tgtcccatat cccaaaatcc ttaaatgtta ttcttttaca
     9181 atctttgccc aggaatcctt caaccctact attctgttga agacaggcct ttccttcgta
     9241 aagtccttat tatcagaaca gaaataaccc atcctcagga actgatagcc ttttcctgca
     9301 gaggcttccc tcaatactgg ttcaacctta cagccggtca acacttccag tgaatcggtg
     9361 ttcagatcat ccttgtattc atgcccctct tccccgggat tcggcactgc aaacaactgg
     9421 tcatacagac ggacttcagc atctatcgca tgttcggcag atatccaatg aatagtaccc
     9481 ttcaccttac gtccgtcggg ggagttccct cccttggatg ccgaatcata ggtacagtgg
     9541 atttctttga tatcacctgt cagcggatcc tttacaacac tttcacactt tatgaaataa
     9601 gcccctttca gcctgacctc tcctcccgga taaagcctga agtatttcct tggcggattc
     9661 tccataaaat cctcctgttc gatatatatc tcacgcagga agggcacctt gcgggtaccg
     9721 ctgtcaggat tctcaggatt gttctccact tcaaaatact ctatctgatt atccggataa
     9781 ttatcgataa taaccttcaa agggcgaagc accgccataa cacggtctgc ctttttattc
     9841 aggtcctccc ttatgcaatg ttcgagaagt gcataatcta cggtgctgtt tgtctttgca
     9901 acgccaatcc tgtcacagaa gtccctgagt gcatcggcag tatagcctct ccttctcaaa
     9961 ccgcataaag taggcatcct ggggtcatcc caaccgctga ctattccggt ttccaccagt
    10021 tccctcaggt atcgtttgct cataacggta taggtcaggt tcagcctggc aaattctatc
    10081 tgccttggct tatgctcagg ttcacattca ttaataaccc aattgtacag aggccggtgg
    10141 tcctcatatt ccaatgaaca gagagaatgg gtaattcctt ccagagcatc agataacgga
    10201 tgctgaaaat catacatcgg atagatgcac catttgtctc ccgttctgtg gtggcttgat
    10261 ttcagtattc ggtatataac agggtccctc atgttcagat ttggtgatgc catatcgatc
    10321 ttggcacgaa gcgttcttga accttcggga aattcacctg ccctcattct cctgaacaaa
    10381 tccaggttct cctcaacagt cctatccctg aaggggctgt ttttacccgg ctccgtaggc
    10441 gttcctctat attctcttac ttcctgctgg cttaaatcac atacataagc tttacccttc
    10501 tttataagat gttctgcaaa ttcataaagc ttttcaaaat agtcggacgc atagtaaagg
    10561 cgatcctccc agtcaaagcc cagccatttg atatcctcca taatggattg cacatattca
    10621 acatcttcct taatgggatt ggtgtcatca aaccttaaat tgcataatcc gcgatatttt
    10681 tccgccgtac tgaaatttat gtacatagcc ttgcaatggc ctatatggag ataaccgttg
    10741 ggctccggag gaaaacgggt atgtacatgg tcatatactt tttcctccag atctatatca
    10801 ataaaatcgt gtataaagtt tgaactctca ttggtatttt cgttcaagga cattacctcc
    10861 ttttttttgc gcaatacatt agtatgctga atattatact atatagattc tgctttttcc
    10921 agtaatctta tgaatatttg gagacgggcg ccatgtatgg
//
LOCUS       DAOQUR010000071        10920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_6754_length_10920_cov_8.162448, whole genome shotgun sequence.
ACCESSION   DAOQUR010000071 DAOQUR010000000
VERSION     DAOQUR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10920
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6754_length_10920_cov_8.162448"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..761
                     /locus_tag="PKI50_10915"
     CDS             111..761
                     /locus_tag="PKI50_10915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT04250.1"
                     /translation="MNKYDTVIFDLDGTLLDTLDDLADSVNYVLEACGFPPREVAEVR
                     NFVGNGVARLVELCIPDGLNNTLYDKCLADFRSHYSMNMQNKTSAYKEIPELLEELSK
                     KGYKLAIVSNKFDKAVKGLNQVYFAKYIKVAVGESEEIRRKPAPDTVFKALEELGSTA
                     DKAVYVGDSEVDVQTARNSGVICVGVTWGFRDREVLEQEGADYIIDSPLELLKIIS"
     gene            complement(804..1376)
                     /locus_tag="PKI50_10920"
     CDS             complement(804..1376)
                     /locus_tag="PKI50_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04251.1"
                     /translation="MINSIKGSPAVNQNYEAKEKHDNRQVENTKAKEDQAVILDLSKP
                     VKKSAGYSKPAARKIDVDEIDRLWKESQKSYETLRKLVEKLISKQGKKLEDLLEGKDV
                     LLVDEETRAAAAQAVSEDGELGVKAVSERIVAFAKAVSGDDKSRIGELKNAIIQGFKE
                     AEKALGGTLPDISQRTYDEVMKKLDEWSEE"
     gene            1538..1810
                     /locus_tag="PKI50_10925"
     CDS             1538..1810
                     /locus_tag="PKI50_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT04252.1"
                     /translation="MEDVKKYEGIAEILKSMAHPVRICIIKGLMEKKCNVTHMQECLE
                     LPQSTVSQHLGVLRAKGIIKGERNGLEITYSVVNEDVKKIINAIFD"
     gene            1828..3507
                     /locus_tag="PKI50_10930"
     CDS             1828..3507
                     /locus_tag="PKI50_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT04253.1"
                     /translation="MADNKLIVIGAVAAGTKAASKAKRDNPNLEVKVFTKERYISYAG
                     CGLPYYIGGIIRDKQELLVRSPEDFKREQGIDIFTEHEVKKIDASGKKVIVEDLKSGD
                     IREYSYDKLIIATGASPIVPPLENINLANIFTLRSVSDAFAVRELLDSGAAKKAVIVG
                     GGFIGLETAENLRNRGMDVTIVELVPHILPPFDEEMALYAQNHMIDRGVRILTGEKVT
                     GFEGSDGKVTAVKTSAGDIEADMVILSIGVRPNTGIAVEAGIETGAAGAIKVNKYMET
                     NIKDIYAAGDCAENTNLITGKPAWYPMGSTANKTGRIAGHNAALEKQEEALKESLEGV
                     LGTTIVRLFEVNAAKTGLSEKDALKEGYEAETVLVPANDTAHYFPGYRMINTKLVVEK
                     STHKVLGAQIVGEGIVDKPIDTIAAAITMGARAEDLAKLDLAYAPPFSMAVGSTILSA
                     NVMLNKLNKRMESVKAPVLPDMLEKGGINVLDVREEEEFFISSIPGSRNIPMNELEAR
                     IGEIDKSKETIILVCKVGKRAYLLYLRLKKLGFTNLRVLEGGICAYPYKLE"
     gene            3522..3746
                     /locus_tag="PKI50_10935"
     CDS             3522..3746
                     /locus_tag="PKI50_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNT04254.1"
                     /translation="MAEKYINARGLQCPGPVVELFKVAKVSEKGDLITIEVTDMAFKK
                     DIAAWCRKTNNELVSLCEGEGTLKAVIRKG"
     gene            3756..4226
                     /locus_tag="PKI50_10940"
     CDS             3756..4226
                     /locus_tag="PKI50_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012958110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNT04255.1"
                     /translation="MSRKTMIMFSGDLDKAMAGLIIANGAAAMGDEVTMFFTFWGLNI
                     LRKAQKIKVRKSFMEKMFGFMMPRGADKLGLSKMNMGGMGTLMMKGIMRKKRVSSLPE
                     LIESAQDLGVKFIACTMSMDVMGIKPEELIDGVEFAGVATYLGEADEANVNLFI"
     gene            complement(4223..4930)
                     /locus_tag="PKI50_10945"
     CDS             complement(4223..4930)
                     /locus_tag="PKI50_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT04256.1"
                     /translation="MKVLKLILPLINLLPESIIRLLGRIITGFLLSKYAELEIHGKEI
                     LAEREDKPTIYISNHLSNIDGVVLNSLLKNNSAAFMAGVKLEKNPFTRFFLKSINHIP
                     ITPGSPDKSAVKSALKHLGSGGSIVIFPEGTRSRTGSLIKVKKGFILLVKLAKVPVVP
                     IALEGTEIVLPINDNDMGSEKLRKAGVIVKIGEPFTLPGKNEHPGAEDWEEICVAYSM
                     KKIAAMLEPKYRGVYGN"
     gene            complement(5123..6085)
                     /locus_tag="PKI50_10950"
     CDS             complement(5123..6085)
                     /locus_tag="PKI50_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04257.1"
                     /translation="MNTIKPLYRIILPILILLSLLSFASCSPQKKPDLEKAQKEAEEK
                     KPPDELDKLKDSIKKVEKALEAIHEENKKPMFMQQEKIEKQASEEGKKQEGEQGGQSG
                     EQGGGGEGSSGGSESGGEQQKQQAQAPKLTPEQMKMKLEQEKYKKFESIKKDVLELHS
                     AWNSYEAKAISDFAMQTTINDFESALNNLTKTVEAQDAYLSLLEINQLYKYLPDFYML
                     YESKVPPDLDRLRFAAKKIMLLSEKQNFTGANDVLLYFENIWMTARPKLKSENAEIVS
                     KFEFAFADLKNAVIAKNDMIVEAKSEVLLKLVDEIEKKAEKSGK"
     gene            6394..7116
                     /locus_tag="PKI50_10955"
     CDS             6394..7116
                     /locus_tag="PKI50_10955"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNT04258.1"
                     /translation="MKTIGIIGGMGPLAAARLFERIVILTKAGCDNEHIPIIIDNNTN
                     IPDRTEAILNNGDNPAYELVRSALRLELMGADAIVMACNTAHYFYEEIIKFVKVPVIN
                     MVEETAKYIIKSNPAVKCAGLLATEGTCKSGIYKRVFEKHGLNLLVPEAEEQQHITEL
                     IYDIKRSREDINPENTIRVVSALRKRGAEVIILGCTELPIAFGMFDICSGYVDSLEVL
                     AMSAIAFAGKDIRYEKINKGLK"
     gene            7122..7811
                     /locus_tag="PKI50_10960"
     CDS             7122..7811
                     /locus_tag="PKI50_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNT04259.1"
                     /translation="MKTARVVFAVLFIILVPPVLLLTDVQMVAYDRDYYRAEYDKYSI
                     PGHIGMSMDNLMEATEKLLLYLDNKRDDLNFKASFNHGEEEFFSPRDKLHMIDVRGLF
                     VRGRLFRDISLIYIAVFIMLLFRGKPFRNQLRKLARHGIAISAAGIAPVIVLIILMKI
                     DFYKYFTVFHEIFFTNDLWLLDPAADRLINIFPQDFFTDMAFSISYLYIAEMAAILIC
                     SLIALKLVKSD"
     gene            7832..8740
                     /locus_tag="PKI50_10965"
     CDS             7832..8740
                     /locus_tag="PKI50_10965"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT04260.1"
                     /translation="MKRLCLLLAFIMLFTSGCFLMPGTERESVKLYYAVKGNAGLDIE
                     NREIEYKDAKSKYANTLEELLKGPSDSGKFETSISRDTKVLSVKVEGDNLTVDFSKEF
                     NVFSGSMHEAAVVSSVVDTMLQFNELKKVRILVEGQELIAPSGEPYGFMEFIDFNIGD
                     MAEKEITLYFADSQAMYVVPEKRTVFVKKDISDAEFFRIVLEELIKGPNTENLYGTIP
                     EGVAVEYTEVEGDLLKVDFSEEMHTNHWGGAAGESMTINSIVNTLTEFENIKRIMPTV
                     DGGPMSIEHMVVEEPLTRNESMIYKP"
     gene            8766..9731
                     /locus_tag="PKI50_10970"
     CDS             8766..9731
                     /locus_tag="PKI50_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNT04261.1"
                     /translation="MELQDSGRLSIFNGNLLYMIVMILFVTVGYIAQQRDFHYGILIT
                     EFLLIALPAIIYVKINGASVKKELRFNSLSLTDAILVIVAAVAAYFAAVFINLIAEIL
                     ISLMGDLIVPDIPFAKNPGEYLVLLFVIAGSAGICEEILFRGFILRSYEKLGMWPDIV
                     FTALLFSVLHLNIQNMAAPFFLGIILGFVVYKTNSIYAGMLGHFINNAVSVTWGYVIM
                     NLPFYESMDIEQLQGGMTTQSLIGASIIFGLILPIAGTILAICLKAIKDRHPEAASPR
                     PDESFVSTMGNIRLSWPLLVSLLIFIGMMIMEILLITNGRRPINF"
     gene            9749..10600
                     /locus_tag="PKI50_10975"
     CDS             9749..10600
                     /locus_tag="PKI50_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT04262.1"
                     /translation="MMDAAVIGGGPAGLSAALNLKIRNKSLVFLGGSENVTGLMKAPQ
                     IDNYPGLKGISGAELVKSFRDHAISMGVEIKKEKAYNILDMGDYFSINAGNEFYEARS
                     VIIATGREKAKYLEGEKEYLGKGIGYCATCDGPLYRNKSIAIISENEEGEEEANFLSE
                     ICSEVFYIPSYNAEPKLKDSIKLLKEKAIGIQGDGNKVNKLTTDKRELKVDAVFIVRD
                     VIPVNQLIEGLELHEGSIKVDRDMAASIKGVFACGDCTGKPYQIAKAVGEGNVAALSA
                     VRYLEKK"
BASE COUNT         3165 a         2044 c         2674 g         3037 t
ORIGIN      
        1 aggcaatcgt tgtaaaagta tgtcgcctgt ccatttccca tttttgcgta tcatgagtgt
       61 atattgtgat ataattatat agtccgggca tgcagtgagg ggggatatga atgaataaat
      121 acgataccgt tatatttgat cttgatggaa cacttcttga tacacttgat gaccttgcag
      181 acagtgttaa ttacgtattg gaagcatgcg gctttccgcc cagagaggtt gcggaggtca
      241 gaaactttgt cggcaatggt gtggcacgat tagtggagct atgtatacct gacggactga
      301 acaatactct gtatgacaaa tgccttgcgg attttcgcag tcattattca atgaatatgc
      361 agaataagac cagtgcatac aaagaaatcc cggagctttt agaagaactg tcgaaaaaag
      421 gttataagct tgcaattgta tcaaataaat ttgacaaagc agtaaaaggg cttaatcagg
      481 tttattttgc caaatatata aaagttgcag tcggagaatc agaggaaata cgcaggaagc
      541 cggcaccgga tactgtgttc aaggcacttg aggaattagg ctctactgca gataaagctg
      601 tatatgtagg cgattcggag gtagatgtgc agacagccag gaattcagga gtcatatgtg
      661 tgggggtaac ttggggattc agggatagag aggtattgga gcaggaaggt gctgattata
      721 ttattgacag tccgttggag ctgttgaaaa taataagctg aaaagaaata taaaaagggg
      781 ggagtttgtc cccctttacc gttttattct tcactccact catcaagctt cttcatgact
      841 tcatcatatg ttctttgaga aatgtccggc agcgtgcccc caagagcttt ctccgcttcc
      901 ttgaagccct ggataatagc attttttaac tcgccgatcc tgcttttgtc atcaccggaa
      961 actgcctttg caaaggcaac tattctttcg ctgaccgcct ttactcccaa ttctccgtct
     1021 tcggaaactg cctgtgcggc tgcagctctg gtttcctcgt ccacaagcag gacatctttt
     1081 ccttccagta aatcctccag ctttttacct tgcttcgaaa tgagtttttc aacaagcttc
     1141 cgcaatgttt cgtaggattt ttgcgattcc ttccaaagcc tgtcaatctc atccacgtct
     1201 atttttctag ctgccggctt gctgtacccg gcactttttt tgacaggctt acttaaatcc
     1261 agaattactg cttgatcttc ttttgcttta gtattctcaa cctgcctatt gtcatgtttt
     1321 tcttttgcct cgtaattttg attaactgca ggacttcctt taatactgtt aatcattata
     1381 ttccctcctt ggtaaataaa tttacatcct tatgatatat atatcggatg aatatagaga
     1441 agatatgaga aatttaataa aaattgctgc ttaagcttga caataatatc acaatatcgt
     1501 aatattagaa tatagcaata tagacggagg tgacttaatg gaagatgtaa aaaagtatga
     1561 aggtattgca gaaatattga aatccatggc ccatcccgta aggatatgca ttataaaggg
     1621 gcttatggaa aaaaagtgca acgtaacaca tatgcaggaa tgcctggagc ttccgcagtc
     1681 cactgtatcc cagcatctgg gtgttctgag ggcaaaggga ataataaagg gagaaagaaa
     1741 cggactggag ataacctaca gtgtagtaaa tgaagatgtg aaaaaaatca tcaatgcaat
     1801 atttgattaa atttaagggg gtaaaccatg gctgataaca aactaattgt aataggtgca
     1861 gttgcagcag gtacaaaggc ggcttcaaag gcaaagaggg acaatcccaa tttggaggtt
     1921 aaggtcttta ctaaagaaag atatatttct tatgccggat gcggcctgcc atattatata
     1981 ggcggaatta taagggataa gcaggagctg cttgtcaggt cccccgagga ttttaaaaga
     2041 gagcagggaa tagacatatt cactgagcac gaggtaaaga agatagatgc atccggaaaa
     2101 aaggtaattg ttgaggacct gaaaagcggg gatataaggg agtactccta tgacaaactg
     2161 ataatagcca ccggagcttc cccgattgta ccgcccctgg aaaacataaa tcttgccaac
     2221 atatttacac tcaggagcgt atctgatgca tttgcagtaa gagaactgct tgactcagga
     2281 gccgcaaaaa aggcggtaat agtcggcggc ggctttatcg gcctggagac ggccgagaac
     2341 ctgagaaaca ggggcatgga tgttactatt gttgagcttg tacctcacat actgcctcct
     2401 tttgatgaag agatggcgtt atatgcacag aaccatatga tagaccgggg agtcaggata
     2461 ctgaccggag aaaaagtcac cggctttgag ggcagcgatg ggaaagtcac cgctgttaaa
     2521 acctcggcag gggatattga agccgatatg gtgatattgt ctatcggagt aaggccaaac
     2581 acgggtattg ccgttgaagc aggcatagag acgggcgcag ccggggcaat caaggtgaac
     2641 aaatatatgg aaacgaatat caaggatata tatgcagcgg gagactgcgc tgagaatacg
     2701 aatctgatta ccggaaagcc tgcatggtat cctatgggtt ctacggcaaa caaaaccgga
     2761 aggatagcag ggcataatgc ggcacttgag aagcaggagg aagctctgaa ggaaagcctg
     2821 gagggagtgc ttggaacaac aattgtcagg ttgtttgaag ttaatgccgc aaagacgggg
     2881 ctttcggaaa aagatgcact aaaggaaggc tatgaggctg aaacggtact tgttccggca
     2941 aatgacacag ctcattattt cccgggctat aggatgataa atactaagct tgtagtggaa
     3001 aaaagcactc ataaggtgct tggagcacag atagtaggag agggtatagt tgataagccc
     3061 atagatacta ttgctgcggc aataactatg ggagcaaggg cagaagacct ggcaaagctg
     3121 gatcttgctt acgcaccgcc tttttctatg gcagtaggtt cgacgattct gtctgccaat
     3181 gtcatgctga acaagctgaa taaaaggatg gagtccgtta aagcaccggt tctgccggac
     3241 atgctggaga agggcggaat caatgtgctt gatgtgagag aagaggagga attcttcata
     3301 tcctcaattc ccggttcaag gaacattccc atgaatgaac tagaagccag gataggcgag
     3361 attgataaaa gcaaagaaac aataattctg gtgtgcaagg taggcaaaag agcatatctg
     3421 ttatatctca ggctgaagaa gctgggattt actaatctta gggttctgga aggcggaatt
     3481 tgcgcttatc cgtataagtt ggaataaagg aggagataca aatggctgaa aaatacatta
     3541 atgcaagggg gcttcaatgc ccggggcctg tagttgagct tttcaaggtt gcaaaggtat
     3601 ctgaaaaagg cgatttgatt acaatagagg ttaccgacat ggctttcaag aaagatattg
     3661 cggcttggtg cagaaagacc aataatgagc tggttagtct ttgcgaaggg gaaggaacac
     3721 tcaaagcagt gatacggaaa gggtaaggta cgattatgag caggaaaaca atgataatgt
     3781 tcagcgggga tctggataaa gccatggcag ggcttattat tgccaacggt gctgctgcca
     3841 tgggtgatga ggtgacaatg tttttcacct tctggggcct gaatattttg aggaaagcgc
     3901 agaagattaa agtgaggaag tcctttatgg aaaagatgtt cggatttatg atgcccaggg
     3961 gagctgacaa gctggggctc tcaaagatga atatgggcgg tatgggcaca ttaatgatga
     4021 agggtataat gagaaagaag agagtaagca gcctccctga gcttattgag agtgcacagg
     4081 acttgggtgt gaagttcata gcctgcacca tgtctatgga cgtaatgggt ataaagccgg
     4141 aggaactgat tgacggagtg gaatttgcag gggtcgccac gtacctggga gaagcggatg
     4201 aggcaaacgt taacttgttt atttaatttc catatactcc cctgtatttt ggctccagca
     4261 tagccgcaat ctttttcatt gagtaggcga cgcatatttc ttcccaatct tcagccccgg
     4321 ggtgttcatt cttcccggga agggtaaaag gctccccaat tttgactatg actccggcct
     4381 tgcggagctt ttcactgccc atgtcgttgt cgttaatggg aagaactatt tcagttcctt
     4441 ccagggcaat gggcactacg ggcacctttg ccagctttac caggaggatg aaacccttct
     4501 ttacttttat aagggagcct gtcctgctgc gggtaccctc ggggaaaatg acaattgagc
     4561 ctccggagcc gagatgcttc agggcagatt tcacagcact tttgtcgggg ctgcccggag
     4621 taatgggtat atggtttatg cttttaagga aaaagcgtgt gaaaggattt ttttccagct
     4681 tgactcctgc catgaaagca gccgaattgt ttttcagcag gctgttcagc acgacgccgt
     4741 caatattgct cagatgattt gatatatata ttgttggttt gtcttctctt tcagctaata
     4801 tttctttgcc gtggatctcc agttccgcat actttgacaa taaaaaaccg gtgattattc
     4861 ttcccagcaa gcggattatg gactcgggca aaagatttat caacggcagg ataagcttta
     4921 acactttcat atgttactcc cggtccgcaa actcaatgtc tgatgaccat taaataagcg
     4981 gtatttatta ttgttccatg cgcgttttca gcgaagctga aatactcgcg cggcaattaa
     5041 ataagaaata taatacgcgt ttgcgcatat tatatcacat tatatataaa atgataagaa
     5101 ccccgaatta agcgggttct tatcatttgc cggatttttc cgcctttttt tctatctcat
     5161 caacaagctt cagcaggact tcggattttg cttcaacaat catatcattt tttgcgataa
     5221 cggcattttt caaatcggca aaggcaaatt caaacttgct gacaatttca gcgttttcac
     5281 ttttgagctt cggcctggct gtcatccata tattttcaaa gtacagaagt acatcatttg
     5341 caccggtgaa attttgcttt tcactcagca gcattatttt ctttgccgcg aacctaagcc
     5401 tgtcgaggtc ggggggaacc tttgactcgt agagcatgta gaaatccggc aggtatttat
     5461 atagttgatt tatttccagc aagctcagat aggcatcctg ggcttcaacc gttttggtca
     5521 gattgttaag tgccgattca aagtcgttaa tggttgtttg catcgcaaaa tcagatattg
     5581 cttttgcttc ataactgttc caggcactat gcagttccaa tacatctttc ttaatgcttt
     5641 caaatttttt gtatttttcc tgctccagtt tcatcttcat ctgttcggga gtgagcttag
     5701 gggcttgagc ctgctgcttc tgctgctcgc cgccgctttc actgccgccg ctgctgcctt
     5761 cgccgcctcc gccctgttca ccggactggc ctccttgctc cccttcctgc ttcttgccct
     5821 cttcggaagc ttgcttctca attttttcct gctgcatgaa cattggcttt ttgttttcct
     5881 catgtattgc ctcaagggct ttttccactt tttttatgct gtccttaagc ttatccagtt
     5941 catcaggagg ctttttttct tccgcttctt tctgcgcctt ctccaaatcc ggcttcttct
     6001 ggggggaaca tgaagcaaag cttaataagg acaataaaat aagtatcgga agtattattc
     6061 tatataaagg ctttatcgta ttcaaagaaa tcacctcttt aactttatta ttccttaatt
     6121 attctttaat ataatttaag aagcggtttt gagtccttaa tgacaactaa atatctttcc
     6181 atttatggaa tacaactgaa aatttttttg aggacactgg tgggacacgg ggacgtttct
     6241 cctgggacac ggggacgttt ctcctgtccc gctgaaggcg ggacaggaga aacgtccccg
     6301 tgtcccagga gaaacgtccc cgtgtcctct tgttatatga ggattctatt tgttataata
     6361 attgtaatga tcgaataaca gaggagaaaa catatgaaga ctatcgggat aataggcgga
     6421 atggggccgc tggctgccgc caggctgttt gaacggattg taattcttac aaaagccggc
     6481 tgtgataatg aacatattcc aataataatt gacaataata caaatatacc cgacaggaca
     6541 gaagccatac tgaacaatgg ggataatccg gcctatgagc tggtcagatc cgcactgagg
     6601 cttgaactta tgggggcgga tgctatagta atggcctgta ataccgcgca ctatttttat
     6661 gaagagatta tcaagtttgt caaggttcct gttataaaca tggtggagga gactgccaaa
     6721 tatattatta aaagcaatcc ggccgtaaag tgtgcgggtt tgcttgccac agaaggcacc
     6781 tgcaagtcgg ggatatacaa gagggtattt gaaaaacacg gattgaatct gctggtgcct
     6841 gaagcagaag aacagcagca tataacggag ttgatctatg atattaaaag aagcagggag
     6901 gatataaatc cggagaatac aatcagagta gtatctgctc tgagaaaaag aggtgccgaa
     6961 gtgataatcc ttggttgtac agagcttccc atagcctttg gcatgtttga catatgcagc
     7021 ggctatgtag actctctgga ggttctggca atgagcgcaa tagcctttgc agggaaggac
     7081 atcagatatg agaaaataaa taagggtttg aagtgatgca tatgaaaaca gcaagagtgg
     7141 tttttgcagt cctgtttatc atattggtgc cgccggtatt gctgcttacg gatgtgcaga
     7201 tggtggctta tgacagggat tactacaggg ctgaatacga taaatacagc atccccgggc
     7261 atattggcat gagcatggat aatcttatgg aggcaacgga gaagcttctt ttatacctcg
     7321 acaacaaaag ggatgatttg aatttcaaag cctcatttaa ccatggcgag gaagaattct
     7381 tcagccccag ggataagctg catatgattg atgtcagggg actttttgtc agggggcgct
     7441 tattcagaga tatttcgctg atttatattg cggtattcat tatgctcttg ttcaggggaa
     7501 agcctttcag aaaccaacta aggaagcttg cccgtcatgg cattgcaatt tcggcggcag
     7561 gtattgctcc ggttatagtg cttataatac tgatgaaaat tgatttttac aagtatttta
     7621 cggtatttca cgagatattt tttacaaatg acctgtggct gttggatccg gccgcagaca
     7681 ggctgataaa tatttttccc caggatttct ttactgatat ggctttcagc atatcatacc
     7741 tgtacattgc agaaatggct gccatactga tttgcagctt gatagccctg aagcttgtca
     7801 agtccgatta aactatcatg gaggttttat tatgaaaagg ttatgtctat tgcttgcttt
     7861 tattatgctg tttacctcag gctgctttct gatgccggga acagaaaggg aaagcgtcaa
     7921 gctgtattat gctgtaaagg gcaatgccgg cttggatata gaaaacagag aaattgaata
     7981 taaggatgcg aaaagcaaat atgcaaatac ccttgaggag ctgttgaaag gcccttccga
     8041 cagcggcaaa ttcgaaacaa gcatcagcag ggatacaaaa gtgctgagtg tcaaggttga
     8101 aggagataac ctgacagttg acttttcaaa ggagttcaat gttttttcgg ggagcatgca
     8161 cgaagcggca gtggtatctt ccgtagttga tactatgctg cagtttaatg agcttaagaa
     8221 agtgagaata cttgttgaag gacaggaact gattgctccc agcggagagc catacggatt
     8281 catggagttc atcgatttca acatcggaga tatggctgaa aaagagatta ctctatattt
     8341 tgccgactcc caggctatgt atgtggttcc ggagaaaaga actgtttttg tgaaaaagga
     8401 cataagcgat gctgaatttt tccggatagt gcttgaggaa ctcattaagg ggcccaatac
     8461 cgaaaacctg tacgggacaa taccggaagg agttgcggtt gaatataccg aggtggaagg
     8521 agatcttctg aaggttgact tttcggagga aatgcatacc aatcactggg ggggagcagc
     8581 aggagaaagt atgaccatta actccatagt caacacattg acggaatttg agaatatcaa
     8641 aaggataatg cctacagtgg acggaggccc tatgagcata gagcacatgg tggtggaaga
     8701 acctctgaca aggaatgaaa gtatgatata taagccatag gcacgggatt acaggaggat
     8761 taatattgga gctgcaagat tcaggcaggt tgtctatttt caacggcaat cttttatata
     8821 tgatagtaat gattctgttt gtgacagtag ggtatattgc acagcagagg gattttcatt
     8881 atggaattct tataaccgaa tttcttttga ttgccctgcc tgcaatcata tatgtgaaaa
     8941 taaacggtgc ctcagtgaaa aaagagctga ggttcaacag cttgagcctc actgacgcaa
     9001 ttcttgtaat tgttgctgcc gttgccgcat attttgcggc tgttttcatc aacctgattg
     9061 cagaaatcct tataagcctg atgggggatc ttattgtacc tgatataccc tttgcgaaaa
     9121 acccgggaga gtatttagtg cttctttttg ttattgcagg ttctgcgggc atttgcgaag
     9181 aaatattgtt caggggcttc atattgaggt cctatgaaaa gctgggaatg tggccggata
     9241 tagtttttac ggcactgctt ttttctgtat tgcatcttaa tattcagaac atggcggcac
     9301 cgttttttct gggtataata cttggttttg tggtatacaa gacgaattcc atttatgcgg
     9361 gaatgctcgg gcatttcatc aacaatgcgg tttcggttac ctggggctat gtaattatga
     9421 acctgccctt ttatgagagc atggatattg aacagcttca gggaggcatg accacacaat
     9481 ccctaatagg cgcctcaatt attttcgggc tcatattgcc cattgcgggt acaattctgg
     9541 caatttgcct gaaggcgata aaggacaggc acccggaagc ggcctccccg aggcctgatg
     9601 aaagcttcgt aagtactatg ggaaacataa ggctgtcctg gcctttatta gtatctcttt
     9661 taatattcat cggaatgatg attatggaaa ttctgcttat tacaaatgga agaagaccaa
     9721 taaatttcta aagaaggaat gatgaaaaat gatggatgca gctgttatcg gaggcgggcc
     9781 tgccggcctt tccgccgcat tgaacctaaa aataagaaac aagagccttg tatttctcgg
     9841 aggcagtgaa aatgtaacag gccttatgaa agcgcctcag atagacaact atcccggcct
     9901 gaaaggcata agcggagcgg agcttgttaa gagcttccgg gaccatgcaa tttcaatggg
     9961 agtggaaata aaaaaggaaa aagcatataa catacttgac atgggagatt atttctcaat
    10021 aaatgccgga aatgaattct atgaggcgcg gtcagtcatt attgccacag gacgtgaaaa
    10081 agcaaagtac cttgagggag agaaggaata cctgggaaaa gggataggtt attgcgccac
    10141 ctgcgacggc ccgttataca ggaacaagtc gatagctata atttcagaga atgaagaagg
    10201 ggaagaagaa gctaattttc tttccgagat ttgctccgag gtattctata taccttctta
    10261 taatgcagag ccgaagctta aggacagtat taaactgctt aaggaaaagg ccataggaat
    10321 tcaaggggac ggaaataaag tcaacaaact gactaccgat aaaagggaac ttaaagtgga
    10381 tgcggtattc atcgtcaggg atgtaatacc tgtaaaccag cttatagaag gattggagct
    10441 tcatgagggc tctataaagg tggataggga tatggctgca agcattaagg gtgtttttgc
    10501 ctgcggggat tgtaccggaa agccttatca gattgcgaaa gccgtgggag aaggaaatgt
    10561 ggcagccctg agtgcggtgc gatatctcga aaaaaaataa tattgacagc ataaggaagt
    10621 aataatataa taatactaag ataacatata ccccgtatag gtatataata tattgataat
    10681 tacatataat attaaggggt gaaaaaatgc ttagtaagaa tatggttata gacgatatcc
    10741 tttataaata tccgcagctt gagacagtgt tcaaggaaca cggaataaag tgcttcggat
    10801 gaggcggtgc tctgtatatg acagtagagc aggctgctct cagatatcgg atagatccgg
    10861 aagaactggt ggacgacttg aataagaatt tgcagtagca ttgccgtcca ttggacggct
//
LOCUS       DAOQUR010000072        10597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7014_length_10597_cov_11.341965, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000072 DAOQUR010000000
VERSION     DAOQUR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_10597_cov_11.341965"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..903
                     /locus_tag="PKI50_10980"
     CDS             163..903
                     /locus_tag="PKI50_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042581.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNT04263.1"
                     /translation="MNQENRKSIALILMALILSAGLIISTYIVTDGIVEIKGNRTITV
                     TGSAKQQIKSDVIEWTGSFSCQSKLMPEAYAKLKSDSELVKKYLKEKGINEEDMVLSS
                     IDTNPIYEVNYNGQYTNNIIAYRLYQRVSISSADVDKLSRIAREATELINEGIEFQSF
                     SPQYYYSKIADLKVSMLGEATKDAKNRAEQISQNTGSSIGALKSAKMGVFQITPLYST
                     EVSDYGINDTSSLEKEITAVVTCTFEIK"
     gene            934..2454
                     /locus_tag="PKI50_10985"
     CDS             934..2454
                     /locus_tag="PKI50_10985"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNT04264.1"
                     /translation="MKEFNDAKNGFFELVKRIKYYNDAIGVLFWDLKTGAPKKGVPYR
                     AEAIGMLSEEAFRLSTSDAMGEYLAYFDEPDRSSSLDSIMKALVRECRKEYDKYKKIP
                     EVKYKEYVILTSEAECVWEDAKKNNDFELFKPYLEKIVGYNLEFIELWGYKENKYDTL
                     LDLYEPGMTVEKLDAIFSELRSRIVPLVAKIKESAYQPEDQFLKQYFDIGKQEEFGLY
                     ILGRMGYDFDAGRLDESEHPFTTGINPGDVRITTHYYPNDFISAVMSSMHEGGHALYE
                     QNISADLYGTLLCDGASMGLHESQSRFWENIIGRSRSFWQKYYSRLQDFFPEQLKDVT
                     ADDFYRAINKVEPSLIRIEADEVTYNLHIMIRYEIEKALINEEIKVADLPGVWKEKMK
                     EYLGIIPPDDTKGVLQDVHWAGGSFGYFPSYTLGNIYSAQIYNAAKKEIKNFEELVEK
                     GELIKIKDWLAEKIHKHGKLLKPEEILLQVAGEGINPKYLIDYLENKYKEIYKLEF"
     gene            2734..3900
                     /gene="hisZ"
                     /locus_tag="PKI50_10990"
     CDS             2734..3900
                     /gene="hisZ"
                     /locus_tag="PKI50_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNT04265.1"
                     /translation="MQSFEVPQGMRDILTEESSRRTILQERLQHYMHSCGFGRVETPL
                     FEYYELFSGGISPVDDESIVKTIDRNGRVVVLRPDMTIPTARVASTNLKGQRKPLKLF
                     YAGSVYRADKKNRGAGREFCQVGAEIYGCSSKWLDIETLTMAKESFRVAGIEDYKIDI
                     GHVGIIKGIFEEMSLTEEKKSQIISLISEKNLVELENEVSALPLDSSSKEIICRLPCL
                     FGKPEDVFKGMDEIIVNETVKESVEYLLQIYGKCKDLGLGSNIIIDAGMTGNMKYYTG
                     LIFKAYARGAGDVVISGGRYDGLLKEMGSDAAASGFAIYIDNMLEAVTEKFEEQKGSK
                     ILVIFSESRFVEALKYSEGRRNNDAAVNMVNRGDISDPEQYCRQYGCNEVVIFE"
     gene            3913..4569
                     /gene="hisG"
                     /locus_tag="PKI50_10995"
     CDS             3913..4569
                     /gene="hisG"
                     /locus_tag="PKI50_10995"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNT04266.1"
                     /translation="MLKIAVAKGRIAEKVSELLLYTSDYRDIIDLNSRKLVFVDKNKE
                     IVFFLAKPSDVPVYVGSGAADIGIVGKDVLMEAKDSAYEIMDLKACRCRIVLAGPSGK
                     SGVRPIVRKVATKYPRIASEYFNRKSESVEIIKLDGSIELAPIVGLSDAIVDIVETGN
                     TLRENGLVVYENICDITARMIVNRVSYKMKNKSINIFASAVSEAIEGGGANAQSAVMG
                     "
     gene            4544..5839
                     /gene="hisD"
                     /locus_tag="PKI50_11000"
     CDS             4544..5839
                     /gene="hisD"
                     /locus_tag="PKI50_11000"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNT04267.1"
                     /translation="MLRVLSWDDKSSIEGFLGQKNNGFIPEEVTETVKKIVRDVRDRG
                     DSAVLEYSRRFDNVNDIKDFRISSRALEKLAGEAEAGFIEAIGKSVDNIKRFHINMAD
                     RDFSIFTDNGSVLGSRVTPIDKAAIYVPGGKASYPSTVVMCAVPAMIAGVREIIILTP
                     PSDNGSINPYVAATAKLLGIDKVFRIGGAQGIAAAAFGTAAIPKVDKIVGPGNTYVAA
                     AKREVYGFVDIDMIAGPSEIAVVADSSANPGWVAADLLSQAEHDEMARCFLITDSGSF
                     AARVNTEIERQLGQMKRKGIISRALENNSAVIVTDAIEQAADIINRIAPEHLEIITEN
                     PFGVLKDIRNAGAIFLGAYSTEPIGDYIAGPSHVLPTYGTAAFFSPLSTRDFQKRSSL
                     IYYSKEDLAAYGPAAIKIAEAEGLEAHANALKVRLQNDR"
     gene            5829..6893
                     /gene="hisC"
                     /locus_tag="PKI50_11005"
     CDS             5829..6893
                     /gene="hisC"
                     /locus_tag="PKI50_11005"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNT04268.1"
                     /translation="MIDNLVSKRVQELKSYKAESRIQGIALDKNELPWGLDDKVEEAL
                     IKSIKAMKFNRYPDSDYTELKAAISRYAGIEKQSICVGNGSDELISLVLQAFINPGDT
                     IAIYNPTFAMYRIYGSICGARIWKYDLDGNFELKPDEFIASLQEQKPKLVFLCNPNNP
                     TGKELALSEMEEILKNTAGIVVVDEAYFEFSGVTALGFLSGYDNLIILRTFSKAFGLA
                     GLRIGYMLANPLIISYIDRVRSPFNVNAFAQAAAVEVLKNLDKVMERVELIKSERERL
                     IVLLKQLKGIQCFESKSNFILMRSPYSDEIAERLQKAGIFIRKYSSTKLKDCLRVTVG
                     SRGENDKFYETVKEVLYERA"
     gene            6880..7479
                     /gene="hisB"
                     /locus_tag="PKI50_11010"
     CDS             6880..7479
                     /gene="hisB"
                     /locus_tag="PKI50_11010"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNT04269.1"
                     /translation="MKELEGARTAEISRKTDETDVYVSLNLEGNGTYEINTGIPFFDH
                     MLEQLGKHSGFDLVVKAQGDIQVDSHHTVEDVGIVLGQALYQCTRNKRGIARYGSIML
                     PMDDSLCAAAVDFCGRANLVYNCILLQGSVGGFDTENIKEFFKAFVGNSAITLHINLM
                     YGENAHHNAECIFKAAARALKEALRLDSRQGMPSTKGCL"
     gene            7483..8091
                     /gene="hisH"
                     /locus_tag="PKI50_11015"
     CDS             7483..8091
                     /gene="hisH"
                     /locus_tag="PKI50_11015"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNT04270.1"
                     /translation="MKLGIVDYGTGNIYSLQKALKHIGIDTVVSKDAIVLDCCSGIVL
                     PGVGAFCDAAANMSAYGLEQVILNQTAKGKLVLGICLGMQLMYDYSMEGGMHKGLGLI
                     SGRVERIPDTAKVPHMGWNTLSIQKKSNVLEGIGDGDYVYYVHSYYVKPEDSTYVCAC
                     SSYGVDIPAVVVKENIVGMQFHPEKSGETGLKILNNLKELII"
     gene            8088..8804
                     /gene="hisA"
                     /locus_tag="PKI50_11020"
     CDS             8088..8804
                     /gene="hisA"
                     /locus_tag="PKI50_11020"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNT04271.1"
                     /translation="MRIYPAVDIYDGKCVRLKEGKLAKMEMFYENPLDAAKMWEQKGA
                     KALHIIDLNGAFDGKTGNSKVIEKIIRSLSIPVQVGGGLRNVSGIKEAFNTGAARVII
                     GTAAVLDEELLETGLKKYIDKMIVSIDAKDGNVAIKGWVNVSEFSAYNFASKIVGKGF
                     RNIIYTDISRDGLLKGPNIDGIEKMCNVEGAKITASGGITSIDDILAIKEAGAEGAII
                     GKALYAGVLKLEDAIKAAED"
     gene            8806..9564
                     /gene="hisF"
                     /locus_tag="PKI50_11025"
     CDS             8806..9564
                     /gene="hisF"
                     /locus_tag="PKI50_11025"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNT04272.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEAARMYNAAGA
                     DELVFLDITASAESRGTMVDAVRETARDVFIPLTVGGGIDSLEDIDRLLHAGADKVSL
                     NTAAVNNPKLVYEGAKRYGSQCIVVAVDAKFKPGTAGWEVYIRGGRTATGIDVLEWCK
                     TVNELGAGEILLTSIDADGSKKGYDIALTSKVAETVNIPVIASGGAGSREDFLEVLTK
                     GKADAALAASLFHFGELRIGDLKLYLASKGVDVK"
     gene            9831..10457
                     /gene="hisIE"
                     /locus_tag="PKI50_11030"
     CDS             9831..10457
                     /gene="hisIE"
                     /locus_tag="PKI50_11030"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNT04273.1"
                     /translation="MSIDDIKFNEQGLIPAIVQDYKTGEVLMQAYMNRESLEKTLTTG
                     RSWFYSRSRKELWEKGATSGCIQMVKEIFYDCDGDSLLLKAEQAGAACHTGNRSCFYR
                     SLWKAEGSEEAVNIGDVVERLYGKVEDRRKNPVEGSYTCYLFNKGIDKICKKIGEEST
                     EVVIAAKNESKEEAVYEISDLIYHLTVLMDYMDITYEKIGAELLKRAR"
BASE COUNT         3295 a         1929 c         2866 g         2507 t
ORIGIN      
        1 tcgtattaat ttgttttgct tcacagtagg cctgtcaaaa tcataatccg tgtgcgtcga
       61 tatactatac agtctcttgc ggaggattat gcaaacttca gatatatagc attcttctgc
      121 ttttgtgtat agtacctaaa atatattaaa gggggttaaa taatgaatca ggaaaacagg
      181 aaaagcattg cccttatttt gatggccctt atcttatcag caggcttgat aatatcaacc
      241 tatatagtta ccgatggtat agtggagata aagggcaaca gaaccataac ggttaccggc
      301 tcggcgaagc agcagataaa atcagatgtt atagaatgga cagggagctt ttcctgccag
      361 tcgaaactta tgcctgaagc gtatgcaaag ctcaagtcgg attcggagct ggtaaagaaa
      421 tacttgaagg aaaagggtat aaacgaagag gatatggttt tatcctccat agatacaaat
      481 cctatttatg aagtaaatta caacggacag tataccaata acattattgc ctaccgattg
      541 tatcagcggg tgtcgatcag ctctgcggat gttgataagc tgagccgcat agcaagggag
      601 gcaacggagc ttattaacga aggcatagaa tttcagtcct tttctcctca gtactactac
      661 agcaaaattg cagacctgaa ggtcagcatg cttggagagg caacaaagga tgcaaagaac
      721 agggccgagc agatatccca gaatacgggc agcagcatcg gagccttaaa atctgcaaag
      781 atgggggtgt tccagatcac accgttatac tccacagaag tatcggatta tggaataaac
      841 gatacatctt cactggaaaa ggagattacg gcagtagtaa cctgtacctt tgaaataaaa
      901 taggggttat ttatatgaga ggggtagatt aatattaaag agtttaacga tgcgaaaaat
      961 ggtttttttg aactggtaaa aaggataaaa tactataatg atgccattgg tgtgcttttt
     1021 tgggacctca aaacaggagc cccaaaaaaa ggcgtgccct acagagcgga agcaataggc
     1081 atgctttctg aggaagcctt caggctgtct acctctgatg cgatggggga atacctggcg
     1141 tactttgatg agccggatag aagcagcagc ctggattcaa tcatgaaggc actggtgagg
     1201 gagtgcagga aggaatacga caaatacaaa aagataccgg aagtaaaata taaggaatat
     1261 gtgatattga cctctgaagc ggaatgcgta tgggaggatg caaaaaagaa taatgacttt
     1321 gagctgttca agccctacct ggaaaagata gtcggctata acctggagtt cattgagctg
     1381 tgggggtata aggagaataa atacgataca ttgctggatt tgtatgagcc cggaatgacg
     1441 gttgaaaaac tggatgcaat attcagcgaa ttgagaagca gaatagttcc cctggttgca
     1501 aagataaagg aatcggctta tcagccggaa gatcagtttt tgaagcagta tttcgatata
     1561 ggaaagcaag aagaattcgg actgtatatc ttaggaagga tgggttatga ctttgatgca
     1621 ggcaggctgg atgagagtga gcaccctttt acaaccggaa ttaatcccgg agatgtgaga
     1681 ataactaccc attactatcc caatgacttc ataagcgctg tcatgagttc aatgcatgaa
     1741 ggagggcatg ccttatatga gcagaatatt tccgcagact tgtacggtac tctcctttgc
     1801 gacggagcct ccatgggact tcatgaatca caatcaagat tttgggaaaa catcatagga
     1861 aggagccgca gcttctggca aaaatactac agccggctgc aggatttttt cccggagcag
     1921 ttgaaggatg taaccgccga tgatttttac agggccataa acaaggtaga gccgtcactt
     1981 atcagaatag aggccgatga agtcacatat aaccttcata tcatgataag gtatgaaata
     2041 gagaaagccc ttattaacga agagattaag gtggctgacc ttcctggggt atggaaggag
     2101 aagatgaagg aatacctcgg tataataccc cccgacgata caaagggagt gctgcaggat
     2161 gtccactggg ccggcggaag cttcggctat tttccctcct atactctggg gaatatatac
     2221 tccgcgcaga tttacaatgc ggcaaaaaaa gaaatcaaga attttgaaga attggttgag
     2281 aagggtgaac tgattaagat aaaagactgg ctggcagaaa agatacataa gcacggcaag
     2341 ctgctgaagc ctgaagaaat ccttctgcag gttgccggcg agggaataaa ccccaagtat
     2401 ttgattgatt accttgaaaa taaatacaag gaaatttata aacttgaatt ttagtattga
     2461 caacagggct tgttttatat tatagtatat gaaaaccaat ttacataata attgcggagc
     2521 tgattttatg aattccttaa attatttgat taacagaatg catcatcata ggcggagcac
     2581 ttaagccttt caggaaattt cccgtaggca taagtgctat ttgtgcttgt gcctatggga
     2641 tgtaataata aaaccccgta ggtaataaaa ataatacctg cggggttttg ttattttcat
     2701 gaacaataca ttaggcaagg agagatatta tctatgcaaa gctttgaggt tccacaaggc
     2761 atgagggata ttcttactga agaaagcagc aggagaacca tacttcagga aaggctgcag
     2821 cattacatgc attcctgcgg cttcggccgt gtagaaacac ctctttttga gtattatgag
     2881 ctgttttccg ggggcatttc cccggtggat gatgaaagca ttgtaaaaac aatagacagg
     2941 aatgggagag ttgtggttct taggccggat atgacaattc ccacagccag agtagcatcc
     3001 accaacctga agggacagcg gaagcccttg aagctgtttt atgcgggaag tgtatacagg
     3061 gcggacaaga agaaccgggg agcaggaaga gaattttgcc aggtaggggc ggagatatac
     3121 ggatgctcaa gcaagtggct ggacatagag accttgacaa tggcaaagga aagcttcaga
     3181 gttgcgggaa ttgaggatta caagatagat atagggcatg taggcataat caaaggtatt
     3241 tttgaagaaa tgagcttgac ggaagaaaag aaatcacaga ttatctcact gataagtgaa
     3301 aagaatctgg tagagcttga aaacgaggta tccgccctgc cccttgacag cagcagcaag
     3361 gaaataatct gcaggctgcc gtgcctgttc ggaaaacctg aggatgtgtt caagggtatg
     3421 gatgaaatta tagtcaatga gacggtaaag gaatccgttg agtatctgct gcagatatac
     3481 gggaaatgca aggaccttgg ccttggctcc aatataataa tagatgcggg aatgacgggg
     3541 aatatgaagt attataccgg attgatcttc aaggcatatg cccggggagc cggtgatgtg
     3601 gttatttcgg gaggaagata tgacggtttg ctgaaggaaa tgggttcgga tgcggctgca
     3661 tcagggtttg ccatatatat tgacaacatg ctggaagctg tcacggaaaa gtttgaggaa
     3721 cagaagggga gcaaaatact ggtaatattc agcgaaagca ggtttgttga ggctttgaag
     3781 tattccgaag gccgcagaaa caacgatgcc gcagtaaata tggtaaacag aggagacata
     3841 tcggatccgg agcaatactg cagacagtat ggctgcaacg aagtggtaat atttgagtga
     3901 ggagtgtcga aaatgttgaa aatagccgtt gcaaaaggca ggatcgccga aaaggtaagc
     3961 gaactgctgc tttatacaag tgattacagg gatataatcg atctgaattc cagaaagctt
     4021 gtttttgttg ataaaaacaa agaaatagta tttttcctgg caaagccctc cgatgttccc
     4081 gtgtatgtgg gaagcggagc ggcagatata ggaatagtag gcaaggatgt acttatggag
     4141 gccaaagatt cagcctatga aataatggac ttgaaggcct gcagatgcag gatagtgctg
     4201 gcaggccctt ccgggaaaag cggcgtgagg cctatagtaa gaaaagtcgc taccaaatat
     4261 ccgagaatag catcggaata tttcaacaga aagtcggaat ccgtagagat aatcaagctg
     4321 gacggttcaa tagagctcgc tcccattgta ggattgtccg atgcaatagt tgacatagtc
     4381 gaaaccggaa ataccctgag ggaaaacgga cttgtcgtat atgagaatat ttgtgatata
     4441 actgcaagga tgatagtgaa cagggtcagc tacaagatga aaaacaagag cataaatata
     4501 tttgcatcgg ctgtcagtga agctatcgaa gggggtgggg ccaatgctca gagtgctgtc
     4561 atgggatgac aaaagcagca ttgagggttt tttggggcag aagaacaatg gtttcattcc
     4621 ggaggaagta acggagacag taaaaaaaat agtccgggat gtcagggatc gtggagacag
     4681 tgcagttctt gaatacagca gaagattcga taacgttaac gatataaaag acttcagaat
     4741 aagcagccgg gctctggaaa aactggccgg ggaagcggag gccggcttca ttgaggctat
     4801 aggcaagagt gtagacaata taaaaaggtt tcatattaat atggccgaca gggacttcag
     4861 catatttacc gataacggca gtgtgctggg cagcagggtt actcccatag acaaggctgc
     4921 aatatatgtt cccggaggca aagcctccta tccctccact gttgtaatgt gtgcagtccc
     4981 cgcaatgata gccggagtgc gggaaataat catattgact cctccgtcgg acaatggaag
     5041 catcaatcct tatgtagcgg caacggcaaa gcttctggga atcgacaagg tgttcagaat
     5101 aggcggggcc cagggaatcg ccgcagctgc ttttggaacc gcagccattc ctaaggtgga
     5161 caagatagta gggccgggga acacatatgt ggcggctgca aaaagagaag tatacggatt
     5221 cgtggatatt gatatgatag cagggcccag cgaaattgcg gtagtcgcag acagcagcgc
     5281 aaatccgggc tgggttgccg ccgatctgct gtcccaggca gagcatgacg aaatggcaag
     5341 gtgctttctg attaccgatt ccgggagctt tgcagcccgg gtaaataccg aaatagaaag
     5401 gcagcttgga caaatgaaaa gaaaaggaat tataagcagg gccttggaaa ataactcggc
     5461 ggttattgtg acagacgcca tagaacaggc ggcggatata ataaacagaa ttgctccgga
     5521 acacctggag attataacgg aaaatccctt tggcgtgctg aaggatataa gaaatgcagg
     5581 ggcaattttt ttgggagcct attccacaga gcccatagga gattacattg caggccccag
     5641 tcatgtactg cccacttatg gcacggctgc tttcttctcc cccctttcaa caagggattt
     5701 tcagaaaagg tcaagcctga tatattacag caaagaagat ttggcggcat atggccctgc
     5761 cgcaataaaa atcgctgaag cggaaggcct ggaggcccat gccaacgcac tgaaagtgag
     5821 gcttcaaaat gatagataat ttggtttcga aaagagttca ggagcttaaa agctataaag
     5881 cggaatccag gatacagggt atagccctgg ataaaaacga attgccctgg ggcttggatg
     5941 acaaggttga agaggcgctg ataaaaagca ttaaagccat gaaattcaac cgctaccccg
     6001 atagtgacta tacagagctt aaagctgcaa tttcaaggta tgcaggcatt gaaaagcagt
     6061 caatatgcgt aggcaacggc tccgacgagc ttataagcct ggtgctccag gcctttatta
     6121 accccggcga cactatagca atctataatc cgacctttgc aatgtataga atatacggaa
     6181 gcatctgcgg cgccaggata tggaaatatg acctggacgg taattttgaa ctgaagccgg
     6241 atgaattcat tgcttcactt caggaacaaa agcccaagct tgtatttttg tgcaacccca
     6301 acaatcctac cggcaaagag cttgcactgt cagagatgga agaaatatta aaaaatactg
     6361 caggtatcgt agttgtggat gaagcatact ttgaattttc aggagttacg gctttagggt
     6421 ttttatccgg atatgacaac cttataatac tcaggacctt ttcaaaggct ttcgggctgg
     6481 cagggttaag aataggatat atgctggcaa accccttaat tatcagctat atcgacagag
     6541 tccggtctcc tttcaatgtg aacgcatttg cgcaggctgc ggcggtggag gttctgaaaa
     6601 accttgataa agtgatggaa agagttgaac tgataaaaag cgagagggaa aggcttatag
     6661 tattgcttaa gcagcttaaa ggcatccaat gctttgaatc aaagagtaat ttcatactga
     6721 tgcgctcccc ctattccgat gaaatagccg agaggcttca aaaggcaggg atttttatta
     6781 ggaaatactc aagcacgaag cttaaggatt gcctgagagt tacagtcggc agccggggcg
     6841 aaaatgacaa attctatgag acagtaaagg aggtgctgta tgaaagagct tgaaggcgca
     6901 aggacagccg aaatatccag aaagactgat gaaacggatg tatatgtaag cttgaatctt
     6961 gaaggaaacg ggacatatga aataaatacc ggaatacctt tctttgatca catgctggaa
     7021 cagctgggaa agcacagcgg cttcgacctt gttgtgaaag cacagggaga tatacaggtg
     7081 gattcacacc ataccgttga ggatgtgggg atagtactgg ggcaggcgct gtaccaatgc
     7141 acccggaata aaaggggcat tgcaagatac ggcagtataa tgcttcctat ggatgactcc
     7201 ctttgtgctg cggcagtgga tttttgcgga agggcgaacc ttgtatacaa ctgcatcctg
     7261 ctgcaaggct ctgtaggagg atttgacact gagaacataa aggaattttt taaggctttt
     7321 gtcggcaatt ccgcaatcac cctgcatata aacctgatgt acggtgaaaa cgcccaccat
     7381 aatgcggaat gtatattcaa ggctgctgcc agggcattaa aggaagctct gcgcctggac
     7441 agcaggcagg gcatgccttc cacaaagggg tgtttgtaag atatgaaatt gggtattgtg
     7501 gattacggaa ccggcaacat ttacagtctt caaaaggcct tgaagcatat cggaattgac
     7561 actgttgtct caaaggatgc catagtcctt gactgctgtt ccgggatcgt ccttccgggg
     7621 gtaggagcct tttgtgatgc tgctgccaat atgtcagcct atgggctgga gcaggtaata
     7681 ttgaatcaga cggccaaagg caagctggtt ctgggaatat gcctgggtat gcagcttatg
     7741 tatgattaca gcatggaagg cgggatgcac aaagggttag ggctgatatc cggccgggtt
     7801 gaaaggatac cggacacagc aaaggttcct catatggggt ggaacacctt gagcattcag
     7861 aaaaaaagca atgtccttga aggaatagga gacggtgatt atgtatatta cgtccactcc
     7921 tattatgtaa aacccgaaga cagcacctat gtgtgcgctt gcagcagcta cggggttgac
     7981 ataccggcag ttgtcgtgaa agaaaatatt gtcgggatgc agtttcatcc cgaaaaaagc
     8041 ggagagacgg gcttgaagat attgaataat ttgaaggagc tgataatatg agaatttatc
     8101 cggcagttga tatttatgac ggaaagtgcg taaggctgaa agagggaaaa cttgcaaaaa
     8161 tggaaatgtt ttatgaaaat cctttggatg cggcaaagat gtgggagcag aagggtgcaa
     8221 aagctttgca tatcatagac ctgaacggtg cctttgacgg taaaacgggg aacagcaagg
     8281 taattgagaa aatcataagg tcgttgagca taccggtgca ggttggaggc ggacttagga
     8341 atgtaagcgg cataaaagag gctttcaata cgggagcggc aagagtgatt atagggactg
     8401 ccgcggtgct ggatgaagaa cttctggaaa caggactgaa gaagtacatt gacaaaatga
     8461 tagtgagcat tgatgccaag gatggaaacg tagcaataaa gggatgggta aatgtaagcg
     8521 aattctccgc ttacaacttt gcaagcaaaa ttgtagggaa aggttttaga aatattatct
     8581 ataccgacat ttccagggat ggcctgttaa agggccccaa tatcgacgga atagaaaaaa
     8641 tgtgcaatgt tgagggtgca aagataactg catcaggggg catcacaagc attgatgata
     8701 ttctggctat taaggaagca ggagcagagg gagctattat tggtaaagct ttatatgcgg
     8761 gagttcttaa gctggaagat gccattaagg ctgcggagga ttaatatgct tgcaaaaagg
     8821 ataataccgt gtctggatgt aaaggacgga agagttgtca aaggagtgag tttcgtcaac
     8881 ctgagggatg cgggggaccc tgtggaggcg gcaaggatgt ataacgccgc aggggcggac
     8941 gagctggtct ttcttgatat taccgcttcc gcagaaagca ggggcactat ggttgacgca
     9001 gtcagggaga ctgccaggga tgtgttcata ccccttacgg taggaggggg aatagacagc
     9061 ctggaggata ttgacaggct tcttcatgcc ggtgcggata aggtgtctct taacactgcg
     9121 gcagttaaca atcccaagct tgtttatgaa ggagcaaaaa ggtacggcag ccagtgcatt
     9181 gtggtggcag tggatgcaaa gttcaagccg ggtacagccg gatgggaagt ctatatccga
     9241 ggcggaagga cggccacagg aatcgacgtt ttggagtggt gcaagaccgt taatgaattg
     9301 ggcgcagggg aaatactcct aaccagcata gatgcagacg gctctaagaa aggctatgat
     9361 atagcattga catcaaaagt ggctgaaaca gtaaatatac ctgttatagc ttcggggggt
     9421 gcgggcagca gggaggattt cctggaggtg cttacaaaag gtaaggctga cgcggcactg
     9481 gcggcgtcgc tgtttcactt tggagaactg cgcattgggg atttgaagtt gtatcttgct
     9541 tccaaggggg tagacgtaaa ataagaagag tgcgatgtag gtaacgggta acactaaaca
     9601 cacgatattt cacggaatcg ctacgcgatt acacggtaac tattatctat tatatagata
     9661 cataagaaga ttttgatact tgcatcatta ccgataatta tttatgtatc ggaatattta
     9721 tcccgccgtg catttagcgg tttttcgtgt ctcccgtgtt acccgttacc tatggcgcat
     9781 tattcttcaa atgcgaatta aatttgaaat attagatagg agaaattcac atgtcaattg
     9841 atgatataaa atttaacgag cagggcctga tacctgccat agtgcaggat tataaaaccg
     9901 gagaagtgct gatgcaggcc tatatgaaca gggaaagcct ggagaagaca cttacgaccg
     9961 gccgctcatg gttttacagc agaagcagga aggagttgtg ggagaagggg gcaacctcag
    10021 gctgcataca gatggtaaag gagatattct atgactgtga cggggacagc cttctgctga
    10081 aggcggaaca ggcgggagca gcctgccata ccggaaacag aagctgcttc tacagaagcc
    10141 tttggaaggc ggaaggcagt gaagaagcag tgaacatcgg ggatgtagtt gaaagattgt
    10201 atggtaaagt agaggacaga aggaagaacc ctgttgaagg ctcttatacc tgctatcttt
    10261 ttaataaagg cattgacaaa atatgcaaga agatagggga agagagcacg gaggtagtaa
    10321 tagccgcaaa aaatgaaagc aaagaggaag ctgtatatga aatatccgac ctgatatatc
    10381 atctgacagt gctgatggat tatatggata taacctatga gaaaataggg gcggagcttc
    10441 tgaagagagc ccggtagggc cgccatatat ggcggccatg agacatgggg ggacatgggg
    10501 acatggggac gtttggggac acggggacgt ttcccctgtc ccacttccag tgggacacgc
    10561 gaaacgtccc cgtgtcccca aacgtcccca tgtcccc
//
LOCUS       DAOQUR010000073        10533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7073_length_10533_cov_10.436725, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000073 DAOQUR010000000
VERSION     DAOQUR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7073_length_10533_cov_10.436725"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2487
                     /locus_tag="PKI50_11035"
     CDS             <1..2487
                     /locus_tag="PKI50_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNT04274.1"
                     /translation="ISEDNVKIDYLIFSIQTYFSLIIKFLSIEVLTYLSDKKDDGLNV
                     LITDNQSKLKEQLEDIESGGIYKRLGVTNFLEGDFFGWYLSLWDDDIYFEFRDLIEEF
                     RKYDYSSINLEPDTAKDLLKDVYHSLFPKELRHNLGEYYTPDWLAEFLIQEMDIEYYS
                     SKSILDPTCGSGTFIVLLIKNYVSYNKGKIEDKKILNSIINNIKGYDLNPLAVISARA
                     NYVIALGDLFNERVCEIEIPIYLCDSMLTILEQKQLDRDCHTVFTKAKEFPIPVSLID
                     KGIMNKILDILNEAVNLDYTEDNFIQRIKNENIINEEGLNKEKAILKELYSITYNLEK
                     EGLNGIWGNIIKNAFAPIFQKKVDYIIGNPPWIVWQSLPEEYRRSIQKYWHEYRVFEH
                     AGLDARLGSAHDDISVLMTYVIMDNFLKDKGKLGFVINQNIFQSSGGGQGFRKLMIRD
                     SIPVKIERVHDFVKVEPFKDLGASNKTAVFTAIKNEETEFPVDYIVWDKKVNGTVDSS
                     ESLENILTKIIGYKSLKAKPIKEKNSSWIIGTEEELTIYDKMVTCQDVHYKARKGVDT
                     SANCIYWVKVKKGLPNNQVLVDNSPEASRKPIKQVENTPLEEDLVYPLLRGKDVQRWN
                     AETHYNIVLPYTNDGKVIEAEELKVKYPRAYEYFYKKENGFIDILINRAIYKKHYLSL
                     NTKKIAPEYVLYNIGPYTFSPYKVVWKALASGMIATTISTNNNKLIIPDHNVIMIPLY
                     DEDEAYYISGILNAEIVSKFINAYISWFFSTHILENMYIPKFNPNNKIHKKIVELSRE
                     AHDFAIQKDRKLHNTEKQVNEIVESLLLNE"
     gene            2992..4386
                     /gene="cysS"
                     /locus_tag="PKI50_11040"
     CDS             2992..4386
                     /gene="cysS"
                     /locus_tag="PKI50_11040"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNT04275.1"
                     /translation="MKLYNTMTRKKEEFEPMTPGEVKMYSCGPTVYNFFHIGNARPFI
                     IFDILRRYLEYRGYKVTFVQNFTDVDDKIIKRAKDENTTEKEVSDRYIKEYFKDAEAL
                     NIRKADYHPRVTEHIKEIIDIIKRLEANSFTYTVGGDVYYDTAKFVGYGKLSKQNLEE
                     LEVGARVDVNEVKRNPFDFVLWKAQKPGEPAWESPWGMGRPGWHIECSAMSMKYLGET
                     IDIHSGGQDLVFPHHENEIAQSEGATGKPFARYWLHNAFLNINNEKMSKSLNNFFTVR
                     DIEEKFDLEVFRLFMISAHYRSPLNFSAELLDQAKSALERLYNAKNNLVYLMDKSEKK
                     PWVQDEKDFKEALLGYRNKFNEAMDDDLNTADGLAVIFDMVRDINSNVKAESSRELVK
                     VSYDMLMELTGVLGLLYKKEESLDSEIEKLIEERQKARKEKNWALADKIRDDLKEQGI
                     ILEDTPEGVKWKRV"
     gene            4411..5733
                     /gene="rmuC"
                     /locus_tag="PKI50_11045"
     CDS             4411..5733
                     /gene="rmuC"
                     /locus_tag="PKI50_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNT04276.1"
                     /translation="MSDIMLYIVIGLLAINSIILIIIMNTSPAGKLIKLEARLDAIEK
                     SIDNLDRTIMEGMLQNREEISGSIKIFNEMIASQMSEISNMQRNQLDIFSQQLANLTQ
                     TNEYRLDRMRDTIEEKLKYLQEDNSKKLDQMRATVDERLSSTLEQRLGESFRLVSERL
                     EMVHKGLGEMQTLASGVGDLKKVLTNVKARGTWGEIQLGNILEQILTPEQYSKNVATV
                     KGSSERVEFAVKLPGRDIKGTETVWLPIDSKFPQEDYQRLLEAQENGNPVKAEEAAKA
                     MEFKVKGFARDIRDKYISPPWTTDFGIMFLPTEGLYAEILRRPGLCDCLQREYRIVIA
                     GPTTLIALLNSLQMGFRTIAIEKRSSEVWSLLGTVKTEFGKFGDILDKTRKKLQEASN
                     TIEDAARRSRSIGRKLKNVQELASEDAEMLLEEADDEDFSQIASAKED"
     gene            5816..6229
                     /locus_tag="PKI50_11050"
     CDS             5816..6229
                     /locus_tag="PKI50_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HNT04277.1"
                     /translation="MMDSGKLSATDVKMLNPLVLAYAGDSVYDTFVRTLLVFGGHGQV
                     AKLHKMSIEFVKAKAQADILWEINELLTPEEQDIVRRGRNTKSSTMPKNADILDYRYA
                     TGFEALIGYLYLTGQIDRLMEVIRMVINVKTSRQK"
     gene            6326..7156
                     /gene="thyA"
                     /locus_tag="PKI50_11055"
     CDS             6326..7156
                     /gene="thyA"
                     /locus_tag="PKI50_11055"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNT04278.1"
                     /translation="MSKVDKIFIEMCKDILKNGFSTEGQNVRAKWEDGSPAHTIKKFC
                     VINRYDLAEEFPILTLRPTNLKAAIDELLWIWQKKSNNIKYLKSHIWDSWADESGSIG
                     KAYGYQLGIKHKYPEGEFDQVDRVLYDLEHNPYSRRIMTNIYNHSDLHEMHLYPCAYS
                     MTFNVTGRKLNGILNQRSQDVLVANNWNVAQYSILMHMMAQVSGLEVGEFVHVIADAH
                     IYDRHIPLVEELIQRNPYPAPKLLINKEVKDFYDFKVEDFELEGYQSGQQIKGIPVAV
                     "
     gene            7171..7668
                     /locus_tag="PKI50_11060"
     CDS             7171..7668
                     /locus_tag="PKI50_11060"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNT04279.1"
                     /translation="MKAIVAADMNWGIGYKGSLLVRIPEDMRFFKEMTLGKAVIMGRE
                     TFESLPGREPLKDRKNIILSRKEGYGDGRITICHSAEDIFDMLKGYKSEDVFVIGGES
                     VYKQFLPYCTEAYVTKIEHKYEADKYFPDLDSKEEWELVSVSDLHTYKDINYRFTKYK
                     NIKFL"
     gene            7941..8717
                     /gene="rlmB"
                     /locus_tag="PKI50_11065"
     CDS             7941..8717
                     /gene="rlmB"
                     /locus_tag="PKI50_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNT04280.1"
                     /translation="MFKADFRQEEDDDDNINLIEGRNPVLEALKSGREIDKIFVQKGV
                     GEGSIRQIIAIAREKKILIKEVDKAKLDGLSRTRNHQGVIASAALYKYYEVDDILQAA
                     REKGEDPFIVILDEITDNNNLGSVIRTAEAAGVHGIIIPKRRAVGLTPAVAKISAGAI
                     EYVPVAKVTNLNHTIEYLKKEGLWIAGAEMGGEPYYGKDMTGPVALVIGSEGEGLGRL
                     IKENCDYLVSIPMKGKISSLNAAVSAAIILFEIQKQRANR"
     gene            8791..9435
                     /gene="sigH"
                     /locus_tag="PKI50_11070"
     CDS             8791..9435
                     /gene="sigH"
                     /locus_tag="PKI50_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HNT04281.1"
                     /translation="MEVNAEEKYVERYDSMFDEEIVLSARDGNATSLEYIINKYKNFV
                     RAKARSYFLIGADKEDIVQEGMIGLYKAIRDFRNDKLSSFRAFAELCITRQIITAIKT
                     ATRQKHIPLNSYVSLNKPIYDEESDRTLLDILTATKITDPEELIISREELVSIESKIG
                     EILSELELEVLMSYLQGKSYQEIACDLDRHVKSIDNALQRVKRKLEKYLEIKDI"
     gene            complement(9461..>10533)
                     /locus_tag="PKI50_11075"
     CDS             complement(9461..>10533)
                     /locus_tag="PKI50_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT04282.1"
                     /translation="CGRLDICGARHPLIGKSAVPLDLSIGDSYRTLVITGPNTGGKTV
                     ALKTIGLLTLMVQSGLHVPADPGSEFAVFTDILADIGDGQSIEQSLSTFSSHIRNIIS
                     IVECAGSNTLVILDELGAGTDPGEGMGLAIAILEETYRKGAVTIATTHYSEIKHYAYE
                     KDGFENGCMEFDLDTLQPLYSLKIGEPGESNAFLIALRLGMKRDIIERAHEITYKERK
                     AYDEFMPLGRITQVKNTEMVQKHAEQTNKSIKLKEDKKNYEKISRQTSFKIGDCVYIS
                     TMNRTGIVCEQEDGKGNLGVMVMQKKFIVNRKRLSLYINSKELYPEDYDFDVILESKE
                     NRKKRKAMGKRHMEGMTIEIKE"
BASE COUNT         3545 a         1713 c         2453 g         2822 t
ORIGIN      
        1 attagtgaag ataatgttaa aatagactat ttaatattct ctatacagac ctatttttcg
       61 ttaattatta agtttttatc tattgaagtt ttaacatatt tatctgataa gaaagatgat
      121 gggttaaatg tattaataac agataaccaa tcaaaattaa aagaacagct tgaggatata
      181 gaatcaggtg gaatatataa aagattagga gttactaatt ttttagaagg ggatttcttt
      241 ggctggtatt tatctctatg ggatgatgat atttattttg aatttagaga tttaatcgaa
      301 gaatttagga aatatgatta tagtagtatt aaccttgaac cagataccgc taaggatttg
      361 cttaaagatg tctatcacag cttattccct aaagaattaa gacataattt aggagagtat
      421 tatacaccag attggctggc tgaatttttg attcaggaga tggatattga atattattca
      481 agcaaaagca tacttgatcc aacttgtggc tctggtactt ttattgtttt attaattaaa
      541 aattatgtaa gctataataa aggaaaaata gaagataaaa aaattcttaa cagtattata
      601 aataatatta aaggctatga tttaaatcca ttagctgtta tttcagcaag agccaattat
      661 gtaattgcat taggtgattt atttaatgaa agggtttgtg aaatagaaat acctatatat
      721 ctttgtgatt ctatgttaac tatattagaa caaaaacaat tagatagaga ttgtcatact
      781 gtatttacaa aagcaaaaga atttccaatt ccggtaagtc taatagacaa aggtataatg
      841 aataaaatat tagatatttt aaacgaagct gttaatttag attatacaga agataatttt
      901 atacaacgaa taaaaaatga aaatatcata aatgaagagg ggctaaacaa agagaaagct
      961 atcttaaaag aattgtattc tataacgtat aacctggaga aagaaggttt aaatggcata
     1021 tggggcaata ttattaaaaa tgcatttgca cctatttttc aaaaaaaagt agattatatt
     1081 attgggaatc ctccttggat agtatggcaa agtcttccgg aagaatacag aaggtctatt
     1141 caaaaatatt ggcatgaata tagggtattt gagcatgccg gactagatgc tagattggga
     1201 agtgcacatg atgatatatc agttttaatg acatatgtta ttatggataa ttttttaaaa
     1261 gacaaaggta aattgggttt tgtaataaat cagaatatat ttcaatcaag cggaggaggg
     1321 caaggtttta gaaagttaat gattagggac tcaataccag taaaaataga aagagttcat
     1381 gattttgtaa aagttgagcc ctttaaagat ttaggtgcaa gtaataaaac ggcagtattt
     1441 actgcaataa aaaatgaaga aacagagttt ccagttgatt atattgtatg ggataaaaaa
     1501 gtaaatggta cggtagactc aagcgaatca ttagaaaata ttttgacaaa aataatagga
     1561 tataaaagct taaaggctaa gcctattaaa gaaaaaaact cttcttggat tatcggaact
     1621 gaagaggaat taactatata tgataaaatg gtaacttgtc aagatgtgca ctacaaagca
     1681 agaaaaggtg tagataccag cgcaaattgt atttattggg taaaagtaaa aaaaggtttg
     1741 ccaaacaatc aagttttagt agataatagt ccggaagcca gcagaaaacc tattaaacaa
     1801 gttgaaaata cacctttgga ggaagattta gtttatccat tactaagggg aaaagatgtt
     1861 caaagatgga atgctgaaac acattacaat atagttcttc cttatacaaa tgatggtaaa
     1921 gtgattgagg cagaagagct aaaggttaaa tatccaagag catatgaata tttttataaa
     1981 aaggaaaatg gatttattga tattttaata aatagagcta tttataagaa acattattta
     2041 tccctgaata caaaaaaaat agccccggaa tatgtattat ataatatagg cccatataca
     2101 ttttcacctt ataaagtagt ttggaaggct ttggcttctg gtatgattgc caccacaatt
     2161 tctacaaata ataataaatt aattattcct gatcacaatg tcataatgat acctttatat
     2221 gatgaagatg aagcttatta tatatctggg atattaaatg ctgagatagt gagcaaattt
     2281 attaatgcat atatttcatg gtttttttca actcatattc ttgagaatat gtatatacct
     2341 aagtttaatc cgaataacaa aattcacaag aaaatagttg agttatctag ggaagcacat
     2401 gattttgcca tacaaaagga taggaagctt cataatactg aaaaacaagt taatgaaatt
     2461 gttgaatcct tgttattaaa tgagtgatct gctagggcta tgcccaatca aaagcaagta
     2521 tggttgagta aatatggtgt cagattacag tccgtcaaaa taccagggtg agcctatcga
     2581 gccggacgat aggagagaat agtaaggcat acaactttac aggctacaat tggaatattg
     2641 ttataaaaag tattgacata atatctatta actataataa tatatataat tgaaggtgaa
     2701 attacaattg aataaatgat gcgcggatcg ggaagagtaa atcagaaagg catacagaga
     2761 ggaaccttcg tcggttgaga gaggtttcat gatacgctga ttgaagtgca cccgtgagca
     2821 ccggagagga agctgaatgc ttgtaaagtg gtattctccg gcggattacc gttaattctt
     2881 gagagtatca aaagagtgga accgcggaat tgcccgcctc tttaagaggc gggtttatct
     2941 attttgatat atgcctcaag cttaatattt atgtatagga aggtgttaaa aatgaagctg
     3001 tacaacacaa tgacaaggaa aaaggaagag tttgaaccga tgacgccggg ggaagtaaaa
     3061 atgtactcct gcgggccgac tgtctacaac ttctttcata taggaaatgc aaggccattc
     3121 ataatattcg atattctaag gagatatctt gaatatagag ggtataaggt aacctttgtt
     3181 caaaatttta cggatgtaga cgataaaata atcaaaagag ccaaggatga gaacacaaca
     3241 gagaaagagg tatctgacag gtacataaag gaatacttca aggatgctga agccttaaat
     3301 attaggaagg ctgattatca tcccagggtt accgagcata tcaaggaaat aatagacata
     3361 ataaaaaggc tggaagccaa tagctttaca tacactgtcg gcggagacgt atattatgat
     3421 actgcgaagt ttgtcggata cggcaagctt tccaagcaga atctcgaaga gctggaggta
     3481 ggcgccaggg tagatgtaaa tgaggtaaaa aggaatccct ttgattttgt gctttggaag
     3541 gctcaaaagc ccggagagcc tgcatgggaa agcccctggg gaatgggcag gccgggatgg
     3601 cacattgaat gctccgccat gtctatgaaa tacctgggag agactataga tatccattca
     3661 ggaggacagg acctggtatt tccccatcat gaaaatgaaa tcgcacagag tgaaggcgcg
     3721 acgggaaagc cttttgccag gtactggctg cataatgcgt tcctcaatat aaataatgaa
     3781 aagatgtcca aatccctgaa caactttttc acagtaaggg atattgaaga aaaatttgac
     3841 cttgaggtct tcaggctttt tatgatttcg gctcattaca ggagtccttt gaatttcagc
     3901 gccgagcttt tggatcaggc aaaaagtgca ctggaaaggc tgtataatgc aaagaacaac
     3961 cttgtttatc ttatggataa gtcagagaag aagccttggg ttcaggatga gaaggatttc
     4021 aaagaggctc ttttggggta caggaacaag ttcaacgagg ctatggatga tgacttgaat
     4081 accgctgacg gattggctgt tatttttgac atggtaagag atataaattc caacgtcaag
     4141 gcagaatcct ccagggaact ggtgaaagta tcctatgaca tgcttatgga gcttacggga
     4201 gtactgggct tgctttacaa aaaggaagaa agtctggatt cggaaatcga aaagcttata
     4261 gaggaaaggc agaaggcaag aaaggaaaag aactgggcat tggccgacaa aataagagat
     4321 gatctgaaag aacagggaat aattcttgag gatacccccg agggagtcaa gtggaaaagg
     4381 gtataaaatt taaataaagg aagtataacc atgtctgata taatgcttta tattgtcata
     4441 ggcttgcttg caataaattc aattattctt attatcataa tgaataccag cccggctggt
     4501 aagctgataa agcttgaagc caggcttgat gccattgaga agagcattga caatctggac
     4561 aggacgatta tggaggggat gctgcagaac agagaggaga tttcaggctc gataaagata
     4621 tttaacgaaa tgatagcctc tcaaatgtcg gaaatatcca atatgcagcg caaccagctg
     4681 gacatatttt cccaacagct tgcaaatctt acgcagacca acgaatacag gcttgacaga
     4741 atgagggata caatagagga aaagctgaag tatctgcagg aggataacag taaaaagctt
     4801 gaccagatga gggcaactgt ggacgaaagg ctaagctcca cccttgagca gcggcttggg
     4861 gaatccttca ggttggtaag cgaaaggctg gaaatggttc acaagggact gggagaaatg
     4921 cagacactgg cctcgggagt gggggaccta aaaaaggtgc ttaccaatgt caaggccaga
     4981 gggacctggg gagaaatcca gcttggcaac atactggagc agattttgac accggagcag
     5041 tactcgaaga acgtggctac agtaaagggt tcctctgaaa gggttgaatt tgcagtaaag
     5101 ctgccgggga gagatataaa ggggacagaa acggtatggc tgcctattga ttccaagttc
     5161 cctcaggagg attatcagag gctgctggag gcgcaggaga atgggaatcc cgttaaagcg
     5221 gaagaagcgg caaaggctat ggagtttaag gtaaaaggct ttgccaggga tataagggat
     5281 aaatacataa gccccccctg gacaactgac tttggaataa tgtttttacc tacggaaggc
     5341 ctttatgccg agattttaag aaggccgggg ctgtgcgact gtctgcagcg ggaatacagg
     5401 atagttatag ccgggcccac aacattgatt gcattattaa acagcctgca gatgggcttc
     5461 aggactattg cgatagaaaa gcgttccagc gaggtatgga gcctccttgg gacggtcaag
     5521 acagaattcg gaaagttcgg tgacatactg gacaaaacac ggaaaaagct tcaggaggca
     5581 agcaacacaa ttgaagatgc tgcaagaaga tccagaagca taggcagaaa gctgaagaat
     5641 gtgcaggagc tggcatcgga ggatgctgag atgctgctgg aagaagccga tgacgaggac
     5701 ttctcccaga tagcttccgc aaaagaggat tagagattgg aatagtaggt atattatggg
     5761 aaatgattat ttggaaaaga actacaaaaa actgctggag gagctgcatc agcttatgat
     5821 ggacagcggc aagctttcgg ctacggatgt caagatgctg aaccctctcg tgcttgcata
     5881 tgcgggggat tcagtctatg acactttcgt caggaccctg ctggtattcg gaggccacgg
     5941 gcaggttgcg aagcttcaca aaatgtcgat tgagtttgtg aaggcaaaag cccaggcgga
     6001 tattctgtgg gagataaatg agctgttgac tcccgaagag caggacattg tacggagggg
     6061 aagaaataca aagtcgtcaa ccatgccgaa aaacgcagac attcttgact acaggtatgc
     6121 taccggtttt gaagccctga taggatactt gtatctgacc ggacagattg accgcctaat
     6181 ggaagtaata aggatggtaa tcaatgtaaa aacaagccgg cagaagtaga attgtctgga
     6241 catgggatgg attttacggg tagaatgtaa taaataggat gtttttgcaa gcccgcagga
     6301 taaaatgtaa gtgaggtgtt ttgtattgag taaggttgat aaaatattta ttgagatgtg
     6361 taaagatatt ttaaaaaacg gtttttcaac agaagggcaa aatgtgagag ccaagtggga
     6421 ggatggcagt ccggcccata cgattaagaa gttttgcgta atcaataggt atgatcttgc
     6481 agaagagttt ccgattctta cattaaggcc gacaaaccta aaagctgcaa ttgacgaact
     6541 gctttggata tggcaaaaga agtccaacaa tatcaaatac cttaagagtc atatttggga
     6601 tagttgggcg gatgagagcg gatccatagg aaaggcatat ggatatcaac tgggtatcaa
     6661 gcataaatat cccgaaggag aattcgacca ggttgaccgg gtattatacg atttagagca
     6721 taatccttac agcagaagga ttatgactaa tatatacaat cacagcgatt tgcatgaaat
     6781 gcacttatac ccctgtgctt acagcatgac ttttaatgtt acgggcagga agcttaacgg
     6841 aatacttaac cagaggtcac aggacgttct ggttgccaac aattggaatg tcgcacaata
     6901 ttcaattctt atgcatatga tggcacaagt tagtggcctt gaggttgggg agttcgtgca
     6961 cgttattgcg gatgcccata tatacgacag gcatatcccg ctagttgagg agcttattca
     7021 aaggaaccct tatccggcgc caaagctgtt gataaacaaa gaagtaaaag acttttatga
     7081 cttcaaggtt gaagattttg agttggaggg gtaccagagc ggccaacaga tcaaaggtat
     7141 tcctgttgct gtgtaataag gagagatggc atgaaggcaa ttgttgctgc agacatgaat
     7201 tggggaatag ggtataaagg cagcttattg gtgaggattc ctgaggatat gaggtttttt
     7261 aaagagatga cgttggggaa agctgtaatt atgggaagag aaacctttga atcattaccg
     7321 ggcagggagc cccttaaaga cagaaaaaac atcatattaa gcaggaagga aggctacggg
     7381 gacggcagaa ttactatatg tcattcggca gaggatattt ttgacatgct gaagggatat
     7441 aagagtgagg atgtgtttgt aatcggcgga gaatcagtat ataaacagtt tctgccctat
     7501 tgtaccgagg cttatgtaac aaaaatagag cacaagtatg aagcagacaa atatttcccg
     7561 gatttggaca gcaaagagga atgggaattg gtatcagtaa gtgatttgca tacatacaag
     7621 gatatcaatt ataggtttac aaaatataaa aacattaaat ttttataaaa aaattacaga
     7681 taataagcat atgaataccg gaactatacc cgaggcaaaa ctaactataa aggatgagaa
     7741 ttatggcgtt taataagaat tattcaaaac cttcaaggcc gggaaggccg ggaaaaaccc
     7801 aaaagaaaga cagcaggttt gtaaaggatg aaggcaggta ttccaagggt tctgtcaaag
     7861 ataaaaagac agagaagcct tttgaaaaga aaaggccttt tgaaaaggcc gcaccttatg
     7921 aggcaggtaa gccgaagaat atgtttaaag ctgacttccg gcaggaagaa gatgatgatg
     7981 acaatatcaa tctgattgaa ggaagaaatc ctgtacttga ggcactgaaa tccggcagag
     8041 aaatcgacaa gatttttgta cagaagggtg tcggagaagg ttcaataagg cagataatag
     8101 ccattgccag agaaaagaag atacttataa aggaagtgga caaagctaag ctggacggcc
     8161 tttcaaggac aaggaaccat cagggagtga tagcttcggc agctttgtac aagtattatg
     8221 aagtggatga tattctgcaa gctgcaaggg aaaagggtga agatcccttt atcgtaatac
     8281 ttgatgaaat aaccgacaac aataatcttg gctcagtgat tcgaacagct gaagcggctg
     8341 gagttcacgg cataatcata ccaaaaagga gagctgtagg acttacgcct gctgtggcaa
     8401 agatttctgc aggcgcaatt gaatatgtgc ctgttgcaaa agtaaccaat ctgaaccata
     8461 caatagagta tctgaaaaaa gaaggcctgt ggattgcggg ggctgaaatg ggaggagaac
     8521 catattacgg caaggacatg accggccctg ttgcattggt aatcggcagc gaaggtgaag
     8581 gactcggacg ccttatcaag gaaaactgcg attaccttgt cagcatcccg atgaaaggaa
     8641 agatctcatc gcttaatgca gccgtttctg ccgcgattat actgtttgag attcaaaagc
     8701 agcgggcaaa ccgctaaaac ttgactaaaa ttgaaggtat atgtataata atagtgtggt
     8761 tttctatgtc tgatacatgg aggcgataga ttggaagtaa atgcagaaga aaaatacgtt
     8821 gaaagatacg acagcatgtt tgatgaagaa atagtgctta gtgccaggga tgggaatgcc
     8881 acatctcttg agtatattat aaacaagtac aagaactttg taagggcgaa agccagatcc
     8941 tacttcctga taggcgccga caaggaggac atagtacagg aaggcatgat aggattatat
     9001 aaagccataa gagattttag aaatgataag ctgtcttcct tcagagcctt tgcagagctt
     9061 tgcatcacca ggcagataat aacggctata aagactgcca cgaggcagaa gcatattccg
     9121 cttaattcat atgtttcgct gaataagccc atttatgacg aagaatcaga caggacactc
     9181 cttgacatac ttaccgcaac aaaaataaca gaccccgaag agctgataat cagcagggag
     9241 gagcttgtct caatagaatc caaaattggt gagatattga gcgaattgga gcttgaggtg
     9301 ctgatgtcat atcttcaagg gaagtcctac caggagattg cctgtgattt ggacaggcat
     9361 gtgaaatcca tcgacaatgc acttcagcga gtgaagagaa agcttgaaaa gtatctggaa
     9421 attaaagata tataaaaaaa tgcgtcgtga gacgcatttt ttattccttt atttcaattg
     9481 tcatgccttc catatgcctt ttccccattg cttttctctt ctttctgttt tccttgctct
     9541 ccaatatcac atcaaaatca taatcttccg ggtaaagttc ctttgaattt atataaaggg
     9601 agagcctctt tctgtttact atgaacttct tctgcatcac catgactccg agattccctt
     9661 ttccatcctc ctgctcacac acgattccgg tgcggttcat agtgcttata tacacacaat
     9721 cgccaatttt gaaagaggtt tggcgtgaga ttttttcgta attcttttta tcctccttga
     9781 gtttaatgct tttatttgtc tgttccgcgt gtttttgaac catttcggtg ttcttgacct
     9841 gagttatcct gcctaagggc atgaattcat cgtaagcttt cctttcttta tatgtgattt
     9901 catgggccct ttcaattata tcccgtttca ttcccaatct taaggcaata agaaaagcat
     9961 tgctttcacc cggttctcct atcttcaaac tatacaaagg ctgcagcgta tcaaggtcaa
    10021 attccatgca gccgttttca aatccatcct tttcatatgc gtagtgcttt atctcgctgt
    10081 aatgggtagt ggcaattgtg accgcacctt tcctatatgt ttcttccaga attgcaatgg
    10141 caaggcccat gccttcaccg gggtctgtgc ctgcccccag ttcatccaat atgaccagtg
    10201 tattgctgcc tgcacactct acgatgctta ttatatttct gatgtgggag gagaaggtgc
    10261 ttaaggattg ttcaatgctt tggccgtctc cgatatcggc aagtatgtcg gtaaagacgg
    10321 caaattcact cccgggatct gcaggtacat gaagtcccga ctgtaccata agcgtcagca
    10381 gtcctatagt ttttaaggcc acagttttgc ctcctgtatt agggcctgtt atgaccaaag
    10441 tccggtacga atccccgatg cttaaatcca gaggcacggc tgactttcct attaaggggt
    10501 gcctggctcc gcatatgtcc agcctgccgc agg
//
LOCUS       DAOQUR010000074        10463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7140_length_10463_cov_11.958397, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000074 DAOQUR010000000
VERSION     DAOQUR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7140_length_10463_cov_11.958397"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(457..1401)
                     /gene="arcC"
                     /locus_tag="PKI50_11080"
     CDS             complement(457..1401)
                     /gene="arcC"
                     /locus_tag="PKI50_11080"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNT04283.1"
                     /translation="MSVIKVVVALGGNALQESGKPATAEEQLQVVNKTAEYLAEMSIK
                     GYEMAVVHGNGPQVGRILLASETAAGVTPVMPFDVCGAMSQGYIGYHIQQGLREALRK
                     RGRNIPVVTLVTQVVVDKDDPGFKNPTKPIGAFYTEEEAKRLQAEKGYVMKEDAGRGW
                     RRVVPSPMPKKIVELSAVKTLWDTTIVITAGGGGIPVIENEDGSLTGIAAVIDKDLAA
                     ERLAEDINADILMILTEVERVSINYKKPDQKDLSLLTSAEAEQYIREGHFAPGSMLPK
                     IQAAVSFVKANPGRKAIITSLYKAVDALEGNAGTTIIF"
     gene            complement(1509..2495)
                     /gene="argF"
                     /locus_tag="PKI50_11085"
     CDS             complement(1509..2495)
                     /gene="argF"
                     /locus_tag="PKI50_11085"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNT04284.1"
                     /translation="MAVNLKGRSFLTLMDFSPLEIRYLLDLSRDLKAKKRAGIYNYLL
                     KGKNIVLLFEKTSTRTRCAFEVGALEEGAHVTYLDQSGSQMGKKESLEDTAKVLGRFY
                     DGIEYRGFKQSAVEDLAKYSGVPVWNGLTDVDHPTQILADMLTIEEHVAKPLNKVKVV
                     FCGDTRNNMAYAWMYGCAKMGMHFTALGPEELSPSQDILNKVNEVAKETGAVIEVAHD
                     IDAVKDADVIYTDVWASMGEEDKIPERVKLLTPYKVTMEMLEKTGNPDVIFMHCLPAF
                     HDFETKLAKEMKEKGYDIREVTDEVFRSRHSVVFDEAENRMHTIKAIMVATL"
     gene            complement(2524..3762)
                     /locus_tag="PKI50_11090"
     CDS             complement(2524..3762)
                     /locus_tag="PKI50_11090"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HNT04285.1"
                     /translation="MFYEEREPFLNVTSEIGKLKAVLLHKPGKELERLTPDYLKDLLF
                     DDIPWLRKMRDEHDEFADVMIQRGAKVYYIEDLLAEILTDLDVKRNLINDILINSNIE
                     NHELRELIFSYIFSKTPAEAAEIAIAGLNKKDIQDVDREYSLSDYIRKDYPLYINPIP
                     NLYFMRDPLSIIGNGISINSMQTNARRREPMLIKYIYDYNPLFRKEYSQVWYNYTIPH
                     SIEGGDILVLSKEVVAVGCSERTSANGIEMLARNLFAGDNDIKEVLAVQIPPLRAFMH
                     LDTVFTMVDRDKFTVYPGILDRVDVYRLTPGGRNGIKVTPENDLVAALKKSLKLPAVS
                     LIQSGGGDTITAAREQWNDSTNTLAIAPGVVVTYSRNEASNEVLRKHGIEVLEIDGSE
                     LVRGRGGPRCMSMPLVREDI"
     gene            complement(4148..4420)
                     /locus_tag="PKI50_11095"
     CDS             complement(4148..4420)
                     /locus_tag="PKI50_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HNT04286.1"
                     /translation="MASKNTLSLIKSNIDNYVGERVVLRANKGRKKISIKQGVIEKTY
                     PSIFLVRIEGEVPEDFGRTVSFSYSDILTKSIELFACKDNVKIGCM"
     gene            complement(4665..6044)
                     /locus_tag="PKI50_11100"
     CDS             complement(4665..6044)
                     /locus_tag="PKI50_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT04287.1"
                     /translation="MLFKVYDCEKELSYDTELDELTARITSFNTAEGHEVEYLKALAV
                     MARTELARKTFIYNGKGCERHRGCDICTEPGHCLEYGLADTEIPKGVYDAVASTDRII
                     MLFEGRPIKPFFHYRCGGATENSENVLGNRITYLRRVLCSFCKDNTDNDSDRYFTVTE
                     LEGLLKTRLKKPEGIYCNIRGMFEDVEVDEQGKISRIKIGTKSFRGIEVRELLKLNST
                     RFDYIPVKFLIKCIGTGHGLGLCQCGANSMARSGMSYQEILKYYYTGIRFEQMEVPDS
                     ERPLKGVRIVLDAVHGGEDCDEGNANLDIVLKLKSLLEGQGAEVYLTRSSGEEMVLSD
                     RAAISNDKRPDLFLSIGQNCFSNPTASGTEVYYYRGDSQGEKLSKLIMENVSGSLGLK
                     NRGVRMADFYLLREIKASSVIIQLLYTSNPQDKELLCNQSFRESAAAAIFKGIGAYYE
                     NSRSGAAAH"
     gene            complement(6061..6927)
                     /gene="yabG"
                     /locus_tag="PKI50_11105"
     CDS             complement(6061..6927)
                     /gene="yabG"
                     /locus_tag="PKI50_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HNT04288.1"
                     /translation="MDIKVGDIVARKSYGFDILFKVHDIVTNKETGTIIYLKGLHYRL
                     FADAPESDVVKVPMNRVNEEIHRFEGKIGRLTKARGRRAGFLSKKLLPKREEYLKFEL
                     PGKVLHIDGDKDYLVMSMKYYKEQGISAVGKLVPEADQPGMIKALLEEYKPDIVIITG
                     HDSMQKQEAKGKDSSERYRNSKYFIEAVKEARKYQQSLDELVIIAGACQSYYEGILSA
                     GANFASSPARILIHALDPGMLCRQIAFTPTDTAAPIEDVLKGTTSGVKGIGGIQTRGK
                     FRAGMPGSPYIP"
     gene            complement(7283..8272)
                     /locus_tag="PKI50_11110"
     CDS             complement(7283..8272)
                     /locus_tag="PKI50_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HNT04289.1"
                     /translation="MKIDAIAREFGCRVIYSTPLRAVNLAQTDRGLVIIKETYRDPDK
                     ILYIHGLKEYLHKNGFTRIDRYLLSRYELPFAIYENRIFVMEDYIAGRECSFTNPYDR
                     EAIVKALAQLHNAGRGYLPSTGAAARNNIGKWEKSYREKIEDLTEFKGLAARKRKKGK
                     LDKMFLEDVDFYIEMGWRGFDTLKNSNYADICKKAKKDNVICHHDYTYHNIIIGPGGE
                     VNVIDFDYSCHELPVYDLAALIQKVLKRYSYDVDMALEIIEDYCSIAPLEREDLVLML
                     SLFEFPQRFWRIAERYYKDKTTWEEKTFLAKYNDTVIMKEFILDFVEDFRKYI"
     gene            complement(8361..9425)
                     /locus_tag="PKI50_11115"
     CDS             complement(8361..9425)
                     /locus_tag="PKI50_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HNT04290.1"
                     /translation="MNHLMRMELFMEENRQSDREFLGGYNLDVKLFEAMGFKVKQIIP
                     VRSVYRIVTDKGFYCLKRLRFPMEDMDFIFSALEHLKGKGFNNAYDIVKQENGDDFIS
                     FKGDKYFLTQWIDGRECDFMNPMDLDAAIEVLARLHNASEGFAPVVCPPDRCWYGKWP
                     EYFNDRIEEMKQMKEQVLEKPEKSEIDRIYLDYADMCINDGEEALQILSKTDYRSLSE
                     EAAVKKSFIHHDFAHHNIIHTFDGRIYAVDFDYCIMDIRMHDVGSLILRNMKKSNWDT
                     DKAMDILGSYDRRNPVSSTELKVLVPFFLFPQDFWMISRQYYIERKDWDEEDFADKMS
                     TKSEYTLMRKKFIEEFEKRI"
     gene            complement(9422..10438)
                     /locus_tag="PKI50_11120"
     CDS             complement(9422..10438)
                     /locus_tag="PKI50_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HNT04291.1"
                     /translation="MSGGYEKLKRHDLKLLIENLYGLEVRNMQEGDRGILVYTDRGIK
                     RLKGTESDEAKILFSASAYEHVCNNGFRQISCINRTLNGSCHMKYDKNNYILQDFTKG
                     RVYEANTAEAAAVAGKALAELHKAGEYFIPAPGSRARVDWGKWMEKFKANSISLKKYK
                     ELICLKEKKSEIDKLFLENIDEFLEKMQYAHGLLKESGYLDKVRQSMSCNQITHGEFK
                     KHAIFEGDPEGVFITNFEECSYDICEFDIATLLESFSGKNKDELAAAAAEAYSDVKPL
                     DEGSMKIIKAFLIYPKRFFKVSESIYGRRKNYNEIELAQKLARSIKREKKKEEVIRFL
                     DFGR"
BASE COUNT         2562 a         2669 c         1868 g         3364 t
ORIGIN      
        1 ggaatttatg ccaacatttt tattttaggg gctgttgcgc aatatatgaa gaatgtgaca
       61 taggtaacgg gtaacactaa acacacggct gtttcacgga atcgctgcgc gattacacgg
      121 taactattat atattatgca gctgcataag aagatttcga tacttatatc attatcgata
      181 gttatttatg taacgtaata tttatcccac cgtgaattta gtggctttca gtgttttcag
      241 tgttacatgt tacacataag gaatacccgg atttctggtg tctagttaat ggtgcctgcg
      301 cacgttagtg cgcattattc tacgtatgca aaaaagacgc ggggacgttt cccccgggga
      361 catggggacg tttcccctgt cccacttcca gtgggacacg cgaaacgtcc ccgcgtcccc
      421 gtgtccttaa gaaggacgcc atgtatggcg cccctactaa aatataattg ttgttcctgc
      481 gttgccctcc aaagcatcca ctgccttgta aagggaggtg attatcgctt ttcttcccgg
      541 gtttgcctta acgaacgata cagcggcctg aatcttcgga agcatgctgc ccggtgcgaa
      601 atgcccctcc ctgatgtatt gttccgcttc tgcagaagtc agaagggaaa gatccttctg
      661 atcaggcttc ttgtagttga ttgaaactct ctcaacttcc gtaagtatca taagtatatc
      721 cgcattgatg tcctcggcaa gcctttctgc ggcaaggtcc ttgtcaatta ctgccgctat
      781 gcccgtaaga ctgccgtctt cattttcaat tacagggatt cctccgcccc ctgccgtaat
      841 tactatggtt gtatcccaga gggtttttac tgccgaaagc tcaactatct tcttgggcat
      901 cggggatggg acgactcttc tccagcctct tcccgcatct tccttcatca catatccctt
      961 ttctgcctga agccttttag cctcttcttc agtataaaaa gcaccaattg gcttagtcgg
     1021 gttcttaaag cccggatcgt ccttatcaac aaccacttgt gttaccaatg ttactacagg
     1081 tatgttcctg cccctcttcc tcagtgcctc tcttaagccc tgctgtatat gatatccgat
     1141 atagccctgg ctcattgccc cgcaaacatc aaaaggcata acaggagtga ctcccgcggc
     1201 agtctccgat gccagaagta ttcttcccac ctgaggccca ttgccgtgta ctactgccat
     1261 ttcatatcct ttgatactca tttccgcaag atactcagct gttttgttca ctacctgaag
     1321 ctgttcttct gcggttgccg gctttccgga ctcctgcagg gcatttccgc ctaatgctac
     1381 cacgaccttt ataactgaca taaatccttc cccttcacaa aaaacatttt agtttccgag
     1441 acttgagcct gtcttcgaag acctaactca actcctcgta ccccgtatcc cgtacctcgt
     1501 atctatcttt ataatgttgc caccattatg gctttgattg tatgcatcct gttttccgct
     1561 tcgtcaaata caacagaatg cctgcttctg aatacctcat cagtcacttc cctgatatcg
     1621 tatccctttt ccttcatttc ctttgccagc tttgtttcga aatcatggaa tgccggcagg
     1681 cagtgcatga atatgacatc cggattgccg gttttttcaa gcatttccat tgtaacctta
     1741 tatggtgtaa gcaatttaac tctttctgga atcttatcct cttcgcccat ggacgcccat
     1801 acatccgtgt atatgacatc cgcatccttt acggcatcaa tgtcatgagc tacttcaata
     1861 accgcaccag tctccttggc aacttcattc accttattca atatatcctg actggggctc
     1921 agctcctccg gtcccaatgc cgtaaagtgc atgcccattt ttgcgcagcc gtacatccat
     1981 gcatatgcca tattgttcct ggtatctccg caaaatacaa ccttaacctt gttaaggggc
     2041 tttgcaacat gctcttcaat tgtaagcatg tccgcaagta tctgtgtcgg atggtctaca
     2101 tccgtaaggc cgttccatac aggtactccg gaatatttgg caagatcctc cacagccgac
     2161 tgcttgaagc ctctgtactc tataccgtca taaaaccttc ccagtacctt tgcggtatct
     2221 tcgagggatt cctttttacc catctgtgag ccggactggt caagataggt tacatgggct
     2281 ccttcttcaa gggctcccac ctcaaaagca catcttgttc ttgtagaggt tttttcaaaa
     2341 agaagaacta tgttctttcc cttgagcaga taattatata tgcccgctct ttttttagcc
     2401 ttcaaatcac gggacaggtc caacaagtac ctgatttcca agggtgaaaa atccattaat
     2461 gtaagaaaac ttcttccttt aagattgact gccattgtaa aatctctcct ttaaaacatt
     2521 attctaaata tcttccctga ccaaaggcat gctcatgcac cttgggcctc ctctgcctct
     2581 taccagttcc gacccgtcta tttccagcac ttcaatccca tgctttctaa gaacttcatt
     2641 ggaggcttca tttcttgaat aagttactac aacaccggga gctattgcca atgtattggt
     2701 actgtcgttc cattgctccc ttgcggctgt tatcgtgtct ccgccgccgc tttgtatgag
     2761 gcttaccgca ggaagcttga ggctcttctt aagagctgcc acaaggtcat tctccggtgt
     2821 gacctttata ccatttctgc cgccgggagt aagcctgtaa acatcaactc tgtcaagtat
     2881 tcccgggtac actgtgaact tatccctgtc aaccattgta aataccgtat ccagatgcat
     2941 aaatgccctt agaggaggta tctgaaccgc cagcacctct ttgatatcat tatccccggc
     3001 gaaaagattc cttgcaagca tttcgatgcc gttggccgaa gtcctttcgc tgcagcccac
     3061 tgccaccact tccttgctga gcaccaggat atctccgcct tctatgctgt gcggtatggt
     3121 gtaattatac catacttgtg agtattcttt tctgaaaagc ggattgtagt catatatata
     3181 ttttattaac atgggctctc ttcttcttgc atttgtttgc atggaattga ttgaaattcc
     3241 gttaccgatt atagacaggg gatctctcat aaaatataga ttcggtattg ggtttatata
     3301 taggggataa tcctttctta tgtaatctga gaggctgtat tccctgtcca catcttgaat
     3361 atcttttttg ttaagccctg ctatggcaat ctctgccgct tccgcagggg ttttgctgaa
     3421 tatgtacgaa aatatcaatt cgcgcagttc atgattttct atattgctgt taatcagtat
     3481 atcattaatc aggtttcttt taacatctaa atcagtcagt atttctgcaa gcaaatcttc
     3541 gatataatag acctttgcgc ctctctgtat cataacatcc gcaaattcat catgctcatc
     3601 cctcatcttc ctcaaccatg ggatgtcatc aaacaaaaga tccttgagat aatccggggt
     3661 aagcctctcc aattccttac ccggtttatg cagcagaaca gcttttaatt tgccgatttc
     3721 ggatgtcaca ttcaggaaag gctctctttc ctcataaaac atctgttact ctcctttcaa
     3781 aaggtggata taccgcattg gaatacatct tctcgcctca ttattaccaa tattagtata
     3841 tacaaataga aataactgta caagtggctt aagctctata ttaagacggc ttttcccatt
     3901 ttatcccata cgcttgtata ttaattcaaa tgcactgtga ataattatta ggtgtccgtg
     3961 caatattgcc attatcctta ttcaaactcc tttacactca tttgatagac tttactgtat
     4021 ttatgcattg tatgcataat aatttagata ttggaataaa aaattattta tttcaaacgc
     4081 aaaaaaagac aaaaaaaatt aagaaccctg attgggtctc aatttttgcc ttacaaagct
     4141 atatatatta catgcatcct atctttacat tatccttaca tgcaaatagc tcgatagatt
     4201 ttgtcagtat atctgaatag ctgaatgaaa ctgttctccc gaagtcctca ggtacctctc
     4261 cttcaattcg tactaaaaat atacttggat atgtcttttc tatcacacct tgcttgatgc
     4321 ttatcttttt tctgccttta ttagctctaa gtacgactct ctccccgaca tagttgtcta
     4381 tattgctttt gatgagggat agggtatttt ttgaagccat aaaaaatcac cttccttccc
     4441 aacatcttaa attttatcat aacatatatt atatgtcaac tggaacaaaa tattatatca
     4501 acttatactt taatagtcaa ataactattt ttatttttca gctatttttc ggctattttt
     4561 catcattaca agctattaag tataaactat acagtattat tccccgaatt actcaatatt
     4621 actcaaaaaa agaacaaaaa agaaaattgc tgcgcaattt tattctaatg tgcagctgct
     4681 ccgctgcgag agttttcgta atatgccccg attcccttaa atatcgctgc tgccgcagat
     4741 tccctgaagc tttgattgca taaaagctct ttatcctgcg gattgcttgt ataaagcagt
     4801 tggattatca cagaagatgc cttaatctcc cggagcagat aaaaatcagc cattctcacc
     4861 cctctgttct ttagtccgag agagccgctg acattttcca ttatcagctt tgacagcttc
     4921 tctccttgag aatctcctct gtaataatat acttcagtgc cggaagctgt cggattggaa
     4981 aagcagtttt ggccgataga caggaacaaa tccggccttt tgtcgttgga tatggccgct
     5041 ctgtctgaaa gcaccatttc ttcgccactg ctccttgtca gatatacttc ggcaccctgg
     5101 ccttcaagaa ggcttttaag ctttaaaact atatccaggt tggcattacc ttcatcacag
     5161 tcctctccgc catgaaccgc atccaataca atcctgacac cctttaaagg cctttcggaa
     5221 tcgggtactt ccatctgctc aaaccttatt cctgtataat agtatttcag tatctcctga
     5281 tagctcattc ctgacctcgc cattgaattt gccccgcact ggcacagccc cagcccgtgc
     5341 ccggtaccga tgcactttat caggaatttt accggtatat aatcaaatct ggtggagtta
     5401 agcttcagca gttctcttac ttctatccct ctgaagcttt ttgtgccgat ttttattctg
     5461 cttattttac cttgctcatc aacttcaaca tcctcgaaca ttcccctgat attgcagtaa
     5521 ataccctcgg gctttttgag cctggttttt aaaagcccct ccaattccgt gactgtgaaa
     5581 tacctgtcgc tgtcgttgtc ggtattatct ttgcaaaaag aacacaatac ccttctcaga
     5641 taagttatcc tgtttcccag gacattctcg gagttttcgg ttgctccgcc gcaccggtaa
     5701 tgaaaaaaag gttttatcgg ccttccttca aaaagcatta tgattctgtc tgttgaagca
     5761 acagcatcat aaaccccctt tggtatctca gtatcggcaa ggccgtattc aaggcaatgc
     5821 cccggttctg tacatatatc acagcctctg tgcctctcgc agcctttacc gttatatata
     5881 aaggtttttc ttgccagctc cgttcttgcc ataacggcaa gagctttcag atactcaact
     5941 tcatgccctt ctgccgtatt gaagcttgtt atcctggctg tcagctcatc cagttccgtg
     6001 tcataactca gctctttttc acaatcatac actttaaaca acatcctaat tcctcccctt
     6061 ctatgggata tagggagaac cgggcatccc cgccctgaac tttcctcttg tctgtatgcc
     6121 tccgattccc tttaccccgc tggtggtgcc tttcagcaca tcctctatgg gggctgccgt
     6181 atcagttggg gtaaacgcta tctgcctgca cagcattccg gggtccaggg catgtatcaa
     6241 tattctggca ggggaacttg caaaatttgc ccctgcagac aatatgcctt cataatatga
     6301 ctggcatgct cctgctatta ttaccagttc atccagtgac tgctggtatt ttctcgcttc
     6361 tttgactgct tctatgaaat attttgaatt cctgtacctt tccgatgaat cctttccctt
     6421 ggcttcctgc ttctgcatac tgtcatgccc tgtgataatc actatatccg gcttatactc
     6481 ctccagcaaa gcctttatca tccccggctg gtctgcttcc ggcacaagct ttcctacggc
     6541 gctgatgccc tgttccttgt aatacttcat gctcataacc agataatcct tatcgccgtc
     6601 tatatgaagc accttaccgg gcagctcgaa tttgagatat tcttctcttt tgggcagcag
     6661 tttttttgac aggaacccgg ccctccttcc cctggccttg gtcagcctgc cgattttgcc
     6721 ttcaaagcgg tgtatctctt cattcactct gttcatggga accttaacga cgtcagattc
     6781 gggtgcatcg gcaaaaagcc tgtagtgcag gcccttaagg tatatgatgg tgcctgtctc
     6841 tttgttggtg actatatcat gaaccttgaa cagtatgtca aagccatagg actttcttgc
     6901 tactatatct cccaccttga tatccatatc ccgaccccct gttattaaag cttacggttt
     6961 gaaacgcaag gagcttgaac cgattttgta tagtttaata catagtatgt gattaaggct
     7021 gacaaggtga gcaaaaaata aaaaacagta ttttttactg tttcagtttg caggcaaagc
     7081 tcagaatggg gacaacaatg tatagtcaga gcatacttat acagaaattg ttaatggctc
     7141 aaatctcgct cgacttagaa acaaccgtat gcgaggcatg gaggccgagc aagctgtcac
     7201 ggtcacggac gacctttgac agcgattcga acaatatttt tgctggtttt tatcagtcac
     7261 gaggcacggc tgaattctcg cgttatatgt actttctgaa gtcctccaca aagtccagga
     7321 taaattcttt cattataacg gtatcgttgt atttggctaa aaaggtcttc tcctcccagg
     7381 tggttttatc cttataatac ctctcggcaa tcctccagaa cctctgggga aattcaaaaa
     7441 gcgacagcat cagcaccaag tcctctctct caagaggcgc tatactgcag taatcctcta
     7501 taatctccaa agccatatcc acatcatagc tgtaccgctt cagtactttt tgtatcaatg
     7561 cagccaggtc ataaacgggc agttcatggc agctgtaatc aaaatctata acgtttacct
     7621 ccccccccgg cccgattata atgttgtgat aagtgtagtc atgatggcat atgacattgt
     7681 ctttttttgc tttcttgcat atatccgcat agtttgaatt cttcagggta tcaaagcctc
     7741 tccagcccat ttcaatataa aagtccacat cctccaggaa cattttatcc agctttccct
     7801 ttttcctttt ccttgccgca agccccttga attccgtcag atcctcaatt ttctccctat
     7861 aggacttttc ccattttccg atgttgtttc tcgccgcagc accggtagaa ggcaaatatc
     7921 ccctgcctgc attatgcagc tgtgccagcg ccttaactat cgcttccctg tcataggggt
     7981 tggtgaagct gcattccctt cccgctatat agtcctccat cacgaatatc ctgttctcgt
     8041 atattgcaaa gggcagctcg tatcgggaca gcagatacct gtcaatacgc gtaaatccat
     8101 tcttatggag gtattccttt aagccgtgta tgtacaatat cttatccgga tccctgtagg
     8161 tttcctttat tattaccaat cccctgtcgg tttgagccag attgaccgcc ctcaacggtg
     8221 tgctgtatat caccctgcag ccgaactccc tggcaatggc atctattttc attggcagca
     8281 cctcatagta tacaaattag ctgacatact attagaatat atgaggggct gcggagactt
     8341 ataccgttgt ttcttttata ctatattctt ttctcgaact cttctataaa cttctttctc
     8401 attaatgtat actctgactt tgtgctcatt ttatccgcaa agtcctcttc atcccagtcc
     8461 ttccgttcga tatagtattg cctggatatc atccagaagt cctgaggaaa aaggaagaag
     8521 ggaacaagta ccttgagttc cgtgctgctt acgggatttc tccggtcata gcttcccagt
     8581 atatccatgg ccttgtcggt atcccagttt gacttcttca tatttctcag aataaggctt
     8641 cccacatcat gcattcttat atccataatg cagtaatcaa aatcaactgc gtatattcgg
     8701 ccatcaaagg tatgtattat attatgatgg gcaaaatcgt ggtgtataaa gcttttcttc
     8761 actgccgctt cctcggaaag gctcctgtaa tcggttttgc tcagaatctg cagcgcttcc
     8821 tctccgtcat ttatgcacat atccgcataa tcaagatata ttctgtctat ttcactcttt
     8881 tcaggctttt ccagcacctg ttccttcatt tgcttcattt cttcaatcct atcgttgaaa
     8941 tactccggcc atttcccgta ccagcacctg tcaggagggc atacaacagg ggcaaagccc
     9001 tctgaagcgt tgtgcagcct tgcaagcact tcaatggcag catcaaggtc catagggttc
     9061 ataaagtcgc attcccttcc gtctatccat tgggtaagga aatacttatc ccctttgaag
     9121 cttataaagt catcgccgtt ttcctgcttg actatatcat aggcattgtt gaagcccttg
     9181 cccttaaggt gctccaaagc cgaaaaaata aaatccatat cctccatggg aaaccttagc
     9241 cttttcaaac agtaaaaccc cttgtcggtt actattctgt atacacttct cacgggaatt
     9301 atctgcttga ccttaaagcc catagcctca aaaagcttta catccagatt gtatcctccg
     9361 agaaattccc tgtccgactg cctgttttcc tccataaaaa gctccatcct cattaaatga
     9421 ttcaccgtcc gaaatccaga aatctgataa cttcctcttt tttcttttcc ctttttatgc
     9481 ttctcgcaag cttttgtgcc aattcgattt cgttatagtt cttccttctt ccgtaaatgc
     9541 tttccgaaac cttgaaaaat cttttgggat atatgagaaa ggcttttatt atcttcatgg
     9601 aaccttcgtc caggggcttt acatcggaat atgcttccgc tgccgctgcc gcaagctcat
     9661 ctttgttttt ccccgagaag ctttcaagaa gcgtcgctat gtcaaattca cagatatcat
     9721 agctgcattc ctcgaagttg gttatgaaca ccccctccgg atcgccttca aatatcgcat
     9781 gcttcttgaa ctctccatgg gttatctgat tgcatgacat agactgcctc accttgtcaa
     9841 gatatccgct ttccttcaga agcccatggg catactgcat tttttccagg aattcatcta
     9901 tattttccaa aaacagcttg tcaatttcac ttttcttttc cttgagacaa ataagctctt
     9961 tatacttttt caagctgatg gaatttgcct tgaatttctc catccacttt ccccaatcaa
    10021 cacgggcacg gcttcccggc gcaggtatga agtattctcc cgccttatga agctctgcca
    10081 gggctttgcc ggcaactgca gcagcttcgg ctgtatttgc ttcatatacc cttccctttg
    10141 taaaatcctg gagtatatag ttgttcttat catatttcat atggcagctg ccgttcaggg
    10201 tccggttgat gcagctgatc tgtctgaaac cgttattgca gacatgctca taagctgatg
    10261 ctgagaataa tatcttcgcc tcatcgcttt cggtcccctt aagccttttt attcctctgt
    10321 cggtatagac aagtatacct ctgtcccctt cctgcatatt tcttacttcc aagccgtaaa
    10381 ggttttcgat cagaagcttg agatcatgcc ttttcaactt ttcatatccg ccggacatta
    10441 actatcccct cctaagacgt aat
//
LOCUS       DAOQUR010000075        10441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7155_length_10441_cov_11.243501, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000075 DAOQUR010000000
VERSION     DAOQUR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7155_length_10441_cov_11.243501"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..775
                     /locus_tag="PKI50_11125"
     CDS             122..775
                     /locus_tag="PKI50_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNT04292.1"
                     /translation="MYIDRKEVLRYMGYRGQNIGHTTEKLLNDCIDEVASVSKESFLY
                     EIFDVEHTAEGLCLKGTTLTLAGEDIGKHLANSEKCALMAVTLGLEVDKRITFYSKTD
                     IAKALVFDACATAAAESLCDTVHEKIETEARSMGFETTTRFSPGYGDFSIGIQKELAK
                     VLKAYEGIGLSVNESSIMIPRKSVTAFIGLQREKSDKSGHSCESCDSKNCFFRKGVE"
     gene            775..3150
                     /locus_tag="PKI50_11130"
     CDS             775..3150
                     /locus_tag="PKI50_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNT04293.1"
                     /translation="MGSIFESKELLIFDGGMGTMLQAHGLKAGELPESWNISNPEKIL
                     EIHKAYIDAGADIITTNTFGANRYKLKGSGCSCGEIIESAVKIAKEAAGSKLTAHDIG
                     PIGQLMEPYGTLSFRDAYYAFREQVAAGTAAGADMILIETMSDIYEARAAILAAKENS
                     HLPVLCTMSFQLDGRTLTGTDPLTMVNIISHLGVDALGINCSLGPKEIIPLLAQVLKY
                     STVPVIVQPNAGLPIILDGKTFYDISPEQFGQYMAETAKMGAAILGGCCGTTPEHIKE
                     LRKAVSGLKPVRPEAVKLTAASSATATVILGEGAKIIGERINPTGKKRLKEALRNGDM
                     DYLLKEAVAQRDAGAHILDVNVGLPEIDEKAMMVRAVFELQNIVNLPLQIDSVSPDVI
                     EAAARICSGRPIINSVNGEKKTMDTIFPIAKKYGCLVVALTLDENGIPEKAEDRLKIA
                     EKIVNRAEEFGIGREDIIVDCLVLTASAQQKEVRETIKAVALVKEKLGLKTTLGVSNV
                     SFGLPDRELLNRTFLAMALTAGLDAPILNPMAEAMTDTIKAYNVLWNIDKEAAEYISS
                     RSEVNEQPVFKEKEENRSLKKLIVDGMKEEAELTVKKLLTEINGMEIVDTFLIPALDV
                     AGQKYESGEIFLPQLIQSAETVKRAFEAIKDNMLKNAGEVTGVSKGKIVIATVKGDIH
                     DIGKNIVKILLENYGFEVYDLGKDVAAESIVKKVKEEKALMVGLSALMTTTVKSMEDT
                     VKALRQHCPDTRVMVGGAVLNEDYAKMIGADYYGKDAREAVKIARRFYGCE"
     gene            3238..>4124
                     /locus_tag="PKI50_11135"
     CDS             3238..>4124
                     /locus_tag="PKI50_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT04294.1"
                     /translation="MNNREMKSNMLLMLTAAIWGFAFVAQRVGMQYVGAFTFNGVRFA
                     LGSISLIPLIIYFKNKKTAEQPEETASASALVPGIIAGSVLFFGASLQQAGLAYTTAG
                     KAAFITGLYIVLVPLLGIFLKQRVGLNTWLGVVLAVSGLYFLSVNEDFTIAFGDLLEI
                     IGAFFWAVHILVIDYFTKKVDALRLSCIQFAMCSALSIIIALIFEDISVSGLSQAAIP
                     ILYGGLLSVGVAYTLQVVAQKHAKPSHAAIILSMEAVFAALGGALLLKENMGVRGYLG
                     CALMFAGMLLTQVNFFEKK"
     assembly_gap    4125..4224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4235..5224)
                     /gene="asnA"
                     /locus_tag="PKI50_11140"
     CDS             complement(4235..5224)
                     /gene="asnA"
                     /locus_tag="PKI50_11140"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNT04295.1"
                     /translation="MSEMYSLSIRETEVAIKVIKDFFEAQLAKKLNLTRVSAPLFVER
                     ATGLNDNLNGIERPVSFDALSLKGSELEIVQSLAKWKRLALSRYGFLPGEGLYTDMNA
                     IRRDEELDNLHSIYVDQWDWEKIILREERTPETLKAVAKTIFSVFKDTEELIFKRYPQ
                     FKPALPEEIFFITAQELEDMYPDKSPKERENLITKDKGAVFLMQIGARLKSGTRHDGR
                     APDYDDWSLNGDILFWYPVLQCAVELSSMGIRVDEEALLRQLEISGCMCRKELPYHSS
                     LLKGELPYTIGGGIGQSRICMYFLQKVHIGEVQSSIWPGSMVSQCEKKNIKLL"
     misc_binding    complement(5275..5540)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5688..6068
                     /locus_tag="PKI50_11145"
     CDS             5688..6068
                     /locus_tag="PKI50_11145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT04296.1"
                     /translation="MKSKVTDASKPANKRKIRRIDYYSKIQCIKSTCEGRTEEYEIPL
                     ELTLINISVGGLGIISEKPFEKDAVLILSFKLEEVKYEKVSAKVMWMMKMGDMYRHGL
                     EIMNVSGKLFKHLSMLDNSITTKV"
     gene            6221..6727
                     /locus_tag="PKI50_11150"
     CDS             6221..6727
                     /locus_tag="PKI50_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNT04297.1"
                     /translation="MSLFENWRDTAYNNEQDEKAKKKFWEGYFEKEESIYEKLLSNPE
                     EVVEGTVAELAERYGTDILLFTGFLDGINDSLVNPMELEALEESSSIKLEIDFEKLYY
                     NMLKAKADWLYNLPQWDNILDDGKRKEITKKQRLSGTVIKSKEPGRNDPCPCGSGKKY
                     KKCCGANK"
     gene            6763..7476
                     /locus_tag="PKI50_11155"
     CDS             6763..7476
                     /locus_tag="PKI50_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT04298.1"
                     /translation="MGVFINSERKEISMKYGDLRNKQGITEKEFLARYDAGRYDRPSV
                     TVDMLVFSITGEMKKELKILMVKRGNHPFLGYWALPGGFVNMDESLDAAALRELKEET
                     GIEDICMEQLYTWGDVDRDPRTRVIGCSYMAIVDSSKLAVKAGDDAEDAAWFKVECSS
                     IDQKKPINNVRLVLSNDRDTLEAIIKVDRAFVGNTARVSRVISESRGIAFDHGKIIEY
                     AVESMRNRLEYRDIGLLTE"
     gene            7492..>7797
                     /locus_tag="PKI50_11160"
     CDS             7492..>7797
                     /locus_tag="PKI50_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNT04299.1"
                     /translation="MKRTGLTTIWAAILASCLLIFAFAEDTGKIVQGTESDARIIYNG
                     KEAVLGLKPVLINGSNYLSVRAVSSLFEKNIYWDSSKNEIIITDKTNPQLEYLSSELA
                     "
     assembly_gap    7798..7897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7898..8350
                     /locus_tag="PKI50_11165"
     CDS             <7898..8350
                     /locus_tag="PKI50_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04300.1"
                     /translation="VIMEEYASAKIKGYYKDISDSRILFSFYYNWEGELVKGNYENYS
                     AISILEEEFNNEYADYLKGIHFTFTLKGNDNITEFTAYIQRDRFRKEWNSLSDKSLKN
                     FMEKLCSDIAGEFEECYIYGYIYDTDSNEELAYCEQTVEGDFVFGRED"
     gene            8382..9401
                     /locus_tag="PKI50_11170"
     CDS             8382..9401
                     /locus_tag="PKI50_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNT04301.1"
                     /translation="MNYNEVLDKAKSQIGSKCRVCRECNGVVCRGEIPGVGGKGSGSG
                     FIRSYEKLNEIKLNMDTIYESKEVDTSIELFGKSFAYPVFAAPIGGLQQHYREDSNDY
                     EYSHAIVSGCKNAGTAGFTGDGVKDEFYEQPLKAISEAGGWGIPTLKPWKNDEILRKI
                     KMAEAAGALAAAMDIDAAGLALLAALGKPVSPKPVEEIREIVAGTRLPFIIKGIMTAA
                     GAAKAVEAGAYGIVVSNHGGRVLDHTPAPVEVLPEIYKAVGGKIKIFIDGGIRTGIDI
                     VRVLALGADAVLIGRPYAIAAYGGGAEGVELYTRKIGAELKDAMIMTGCSTLKDIDER
                     VIYKP"
     gene            9560..>10441
                     /locus_tag="PKI50_11175"
     CDS             9560..>10441
                     /locus_tag="PKI50_11175"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNT04302.1"
                     /translation="MEGVKRLDGWAKFKGFIHLLRPKQWIKNLFLFAALLFSRNMDKP
                     QYVMTVLYAFLCFCMISSAVYVFNDISDAEKDRQHPRKRMRPIAAGIIDRREAQILMI
                     ILLPVSVILSIMLDYSFGFIVMTYLLNNVVYTLYVKNIVILDVMSIALGFILRVSGGA
                     VVIGVTISPWLLLCTFLLSLFLGFSKRRNELLMLQADAHNHRNNLEHYSLDFIDNMLS
                     IVTASTLISYSLYTFYASEGKHSMVTILFVLYGIFRYQYIVYNKKMGESPEEIILTDI
                     PLIVDIMLWILVSIIILY"
BASE COUNT         3152 a         1863 c         2580 g         2646 t
ORIGIN      
        1 ataatatacc ccattatttc agcaatgtca agatataaat tttgtacttt tgagaaaata
       61 aattacttat tgctttttag agcagtttgt gtttatcata tattaaattg gagggctgat
      121 tatgtacata gacagaaaag aagtactgcg ttatatgggt tatagaggcc aaaacatcgg
      181 tcatactacg gaaaagctgt tgaatgattg tattgatgag gttgcaagtg tatcaaaaga
      241 aagctttttg tatgaaatct ttgatgtgga gcatactgcg gaaggcttat gcttgaaagg
      301 gactacattg acccttgccg gagaagacat tggcaagcat cttgccaatt ctgaaaaatg
      361 tgcgttaatg gcagtgactc tggggcttga agttgataaa cgcataacct tctattcgaa
      421 gacggacatt gcaaaagccc tggtatttga tgcctgtgca acagcggcag cggaatctct
      481 atgtgatacg gttcatgaga aaatagaaac cgaggcgagg tccatgggat ttgaaacaac
      541 aacaagattc agccccggat acggagattt ttctattggg attcaaaagg aattggcaaa
      601 ggtattgaag gcttacgagg gtataggact gagtgtaaat gaaagcagca taatgatacc
      661 gagaaagtcc gtaacggcct ttattgggct tcagcgggag aagagtgata aatcaggcca
      721 tagctgtgaa agctgcgaca gcaaaaattg ttttttcaga aagggtgtgg aataatgggt
      781 agtatatttg aatcaaagga gttgctgata tttgacggtg ggatgggtac aatgcttcag
      841 gcccatgggc tgaaagccgg ggaactgcct gaaagctgga atatcagcaa tcccgaaaag
      901 atacttgaaa tacataaagc ctatattgac gcaggagcgg atataattac caccaacact
      961 ttcggggcaa acagatataa gctgaagggt tcgggctgca gctgcggtga aatcatagaa
     1021 agtgcggtaa aaatagcaaa agaagcagcg ggaagcaagc ttacagcaca tgatataggg
     1081 cctataggcc aactaatgga gccgtacggc acattatcct tcagggatgc ctactatgcc
     1141 ttcagggagc aagtagctgc aggaacagcc gcaggggcgg acatgatatt gatagagaca
     1201 atgtcggata tatacgaagc cagggcagct atccttgcag caaaggaaaa cagtcatctg
     1261 cccgtattgt gtaccatgtc ctttcagctt gacggaagga ccctgacggg caccgatcct
     1321 ttgacaatgg tgaatataat atcgcatctg ggggtagatg cgctggggat taattgctct
     1381 ctcggcccaa aggaaattat tccccttctt gcccaggtac tcaaatattc aacagtgcct
     1441 gtaattgtgc agcccaatgc cgggttaccg atcattttgg acggcaagac cttttatgac
     1501 ataagccctg agcagtttgg acaatatatg gccgaaacgg caaaaatggg agcagcaata
     1561 cttggaggct gttgcggtac tactccggaa catatcaaag aattgaggaa agctgtatca
     1621 ggactaaagc ccgtcaggcc cgaagcagta aagctcactg cggccagttc ggcgactgca
     1681 acagtgattc ttggagaagg ggcaaaaata ataggggaga ggataaaccc aaccggtaag
     1741 aaaagattga aagaagcgct gcgaaacggc gatatggatt atttgctgaa ggaggcagtt
     1801 gcacagaggg atgcaggcgc ccacatactt gatgttaatg tagggctgcc ggaaatagat
     1861 gaaaaagcaa tgatggtaag agctgtgttt gagctgcaaa atatagtgaa ccttcccctg
     1921 cagattgaca gtgtcagtcc tgatgtaata gaagctgcgg caaggatttg cagcggaagg
     1981 cccataataa actctgtgaa cggcgaaaaa aagacaatgg acactatatt tcccatagca
     2041 aaaaaatatg gatgcctggt tgttgctctc acccttgatg aaaacggaat acctgaaaaa
     2101 gcggaagacc ggctgaaaat tgctgaaaag atagtaaaca gggcggagga gttcggaata
     2161 ggcagggagg atattatagt ggactgcctt gtgcttactg cctcagccca gcagaaggag
     2221 gtccgggaga caattaaggc tgtagccctt gtaaaggaga agctggggct taagactacc
     2281 cttggtgtaa gcaacgtatc cttcggcctg ccggacagag agctgctgaa cagaacattt
     2341 ttagccatgg ctttgaccgc aggccttgat gctccgattc tgaacccgat ggcagaagcc
     2401 atgacagata caataaaagc atataatgta ctctggaata tcgacaagga agcagccgaa
     2461 tacatcagca gccgctctga ggtcaacgaa cagcctgtat tcaaagaaaa ggaagaaaac
     2521 cgaagtttaa aaaaattgat agtagacgga atgaaggaag aagcggagtt aacggtaaaa
     2581 aagcttctta cggagattaa cggcatggag attgtagata ccttcctgat accggccctt
     2641 gacgttgcag ggcagaaata cgagtcgggg gaaattttcc tgccgcagct tatacagtct
     2701 gctgaaactg ttaaaagagc ttttgaagca ataaaggaca acatgctcaa aaatgcagga
     2761 gaggttacgg gggtctcaaa gggtaagatc gtaattgcca cagtcaaggg ggatatacat
     2821 gacataggca agaatattgt gaagatactg ctggaaaact acggcttcga ggtttatgac
     2881 cttgggaagg atgttgccgc agaaagcatt gtgaaaaagg tcaaggaaga gaaagccctt
     2941 atggttggct taagcgccct tatgaccacc acagtaaaga gcatggaaga taccgttaag
     3001 gctctcaggc agcattgccc ggatacaaga gtaatggtag ggggagcagt gcttaacgaa
     3061 gattatgcaa aaatgatagg ggcggattac tatggcaagg atgcgagaga agcggtaaaa
     3121 atagcccgca gattctatgg atgtgaataa gcttcctttt gaattattgc cattgctttt
     3181 ttcggctgat acaagttaaa atagtattgg cagagctaat gataggggga ttaaataatg
     3241 aataatcggg aaatgaagtc caatatgctg cttatgctta ctgcggccat atggggtttt
     3301 gcctttgtag cccagagagt aggcatgcag tatgtagggg catttacttt taatggggtg
     3361 aggtttgcat taggaagtat ttcactgatt ccgttgataa tatatttcaa gaacaagaaa
     3421 actgcggaac agccggaaga gaccgcttca gcaagtgctt tggtaccggg aatcattgca
     3481 gggtcagttt tgtttttcgg agcttctctt cagcaggccg gcctcgcata tacaacagca
     3541 ggaaaggccg cctttataac agggttatat atagttcttg tgcccttgct gggtattttc
     3601 ttaaagcagc gtgtaggttt gaatacctgg ctgggagttg ttctggctgt ttccgggctg
     3661 tattttctaa gtgtcaatga ggactttacc atagcattcg gggatttact ggagattatc
     3721 ggtgcgttct tctgggctgt tcatatactg gttattgatt actttaccaa gaaagtggat
     3781 gcattgaggc tgtcctgtat acaatttgcc atgtgttcgg cattaagcat cataattgca
     3841 cttatattcg aggatatatc agtatccggc ttaagccagg cagcaattcc catactttat
     3901 ggaggcctat tatccgtagg cgtagcatat acgctccaag tggtagctca aaagcacgca
     3961 aaaccttccc atgcagccat aatactcagc atggaagcag tttttgcagc ccttgggggt
     4021 gcactgctcc tgaaggagaa tatgggggtt aggggatatt tgggctgtgc tcttatgttt
     4081 gcaggaatgc tcctgaccca ggttaatttt ttcgaaaaaa aggannnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnaatata aagcttagag cagctttata ttcttttttt
     4261 cgcactggct taccatgctg cccggccata tggaagactg cacttctcca atgtggacct
     4321 tttgcaggaa gtacatgcat attcttgact ggcctatgcc tccgcctatt gtataaggaa
     4381 gctcgccctt cagcagggag ctgtgataag gcaattcctt ccggcacata cagccggata
     4441 tctccaactg gcgcagcaga gcttcttcat cgacacgtat tcccatggag gaaagctcaa
     4501 cggcacattg cagtacaggg taccagaaca atatatctcc gttcagagac cagtcgtcat
     4561 aatcgggagc ccttccgtca tgtcttgttc cggatttcaa tcgggcgcct atctgcataa
     4621 gaaacaccgc tcctttgtcc tttgtgataa gattttccct ttctttgggg cttttatcgg
     4681 gatacatgtc ctccagctcc tgtgcggtaa taaagaatat ttcctcgggc agagccggtt
     4741 tgaactgcgg ataacgcttg aaaattagtt cctctgtatc cttaaaaacg ctgaatatgg
     4801 tttttgccac agccttcaag gtttcaggag tcctttcctc cctgaggata atcttttccc
     4861 aatcccattg atccacatat attgagtgaa gattatccaa ttcttcgtct cttcttattg
     4921 cattcatatc ggtataaagc ccctctccgg gcaggaaccc gtaccttgaa agagcaagcc
     4981 gcttccactt agccagtgac tgtacgattt ccagctccga gcctttcagg gaaagcgcat
     5041 caaaggaaac aggacgttct attccgttca gattgtcatt aaggcctgta gccctttcaa
     5101 caaacagggg ggccgagacc cttgtaagat taagcttttt agccagctgc gcttcaaaaa
     5161 aatctttgat taccttaatt gctacctccg tttctcttat actgagtgaa tacatttctg
     5221 acattttaat acctcctttt tattgctgca aaaataaaaa cccttcatcc caataatagg
     5281 gacgaaaggt tattcgcggt accaccctga ttgactgaag ttatgctcca atccactctg
     5341 ccagtacgga tattctgatg catttcagaa atcgatactg cccattctgt aacggtatgg
     5401 ctccgtccaa gtctactatc caaaggattt cggttggaga ctcaaggctg ttcttcactt
     5461 caggcttagc accgggcttg caccgatccc ggctcgcttt ggcatatcct acaagctact
     5521 cttcccatca ttgtcgaaaa tttatatttt tatcattata ttagctgtat ggcaaaatgt
     5581 caatggttat tttttgtatg aacaggacga ccaatcatat aaaaaggaat actgcttggg
     5641 caatgggatg gtataataat taataatact atatggaggg cagaattatg aagagtaaag
     5701 ttacggatgc ttcaaagcct gccaataaga gaaagatcag aaggattgat tattattcaa
     5761 agatacaatg cattaaaagt acttgcgagg gcaggaccga agaatatgag ataccgcttg
     5821 aacttacact tattaatatt tcagtagggg ggttgggtat aatatctgaa aagccttttg
     5881 agaaagatgc ggtgctgata ctaagcttca agctggaaga agtgaaatac gaaaaagtat
     5941 ctgcgaaggt aatgtggatg atgaaaatgg gagatatgta tcggcacggg ctggaaataa
     6001 tgaatgtatc cggaaagctt ttcaaacacc tgagcatgct tgacaacagc attacaacca
     6061 aggtatgaaa gactggaatc agacaaaaaa ttgcaaatta tactgcaatg taatataata
     6121 attaaggaaa ttcaataagt gcgccgggga aatggcctta ctgcatatcc gaacaggcgt
     6181 atttactata tgataaaatc actggataag gagtagtagc atgagtttgt ttgagaactg
     6241 gagagatact gcatataaca acgagcagga tgaaaaggca aaaaagaagt tttgggaagg
     6301 atactttgag aaggaagaat ccatctatga gaagctgctt tccaatcccg aagaggttgt
     6361 tgagggaacg gtggcggaac tggcagaaag gtacgggacg gatatactgc tgttcacggg
     6421 cttccttgac ggtattaacg acagtcttgt taatccgatg gagcttgagg cgctggaaga
     6481 gtcaagcagc atcaaactgg agatagactt tgagaagctt tattataaca tgctgaaggc
     6541 aaaagcagac tggttgtata atctgcctca gtgggacaat atactggatg acgggaaaag
     6601 aaaggaaata acaaagaagc aaaggctttc aggaacagtg ataaaatcaa aagagccggg
     6661 gagaaacgat ccctgccctt gcggaagcgg aaagaaatat aaaaagtgct gcggcgctaa
     6721 taaataatgc ataaaaaata actcccaaag gaagatttac cgatgggagt ttttattaac
     6781 agcgaaagaa aggagatatc tatgaaatac ggtgatttaa gaaacaaaca gggcattacc
     6841 gagaaggaat ttttggcccg ttatgatgca gggcgctatg ataggccttc cgttacagta
     6901 gatatgcttg ttttttcaat tacgggcgaa atgaagaaag agttgaagat acttatggta
     6961 aaaagaggga atcatccctt ccttggatat tgggcacttc ccggaggttt tgtaaatatg
     7021 gatgaaagcc tggatgcggc agcccttagg gagctgaagg aagaaacggg cattgaggat
     7081 atatgtatgg agcagctata tacatggggg gatgttgaca gagatccccg caccagggta
     7141 ataggctgtt catatatggc tattgttgac agcagcaagc tggccgtaaa ggcaggagat
     7201 gacgcggagg atgcggcatg gttcaaggtg gagtgcagca gcattgatca gaaaaaaccc
     7261 ataaacaatg taaggcttgt attgagtaat gacagggata ccctggaggc aattataaaa
     7321 gttgacaggg cctttgtcgg gaatacagca cgggtaagca gagtaatatc agagtcaagg
     7381 ggtattgctt ttgatcatgg gaagataatc gaatatgcag ttgaaagtat gagaaacagg
     7441 cttgaatata gggacatagg cctgctaacc gaataaaagg aggggtaata tatgaaaagg
     7501 accgggttaa cgaccatatg ggctgctata ttggcatcct gccttttaat atttgcattt
     7561 gccgaggata cgggaaagat tgtgcaggga actgagtctg acgccaggat tatatataac
     7621 ggcaaggaag cggttctcgg attaaagccg gtattgataa acggaagcaa ctatctttct
     7681 gtaagagcgg tatcttcctt gttcgaaaaa aatatatact gggattcaag taaaaatgag
     7741 ataataatta ctgacaaaac aaatccgcaa ttagagtacc tgagttctga gcttgccnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt ataatggagg aatatgcttc
     7921 agcaaaaatc aagggctatt acaaggatat atcggattca agaatattgt tttcttttta
     7981 ctataattgg gaaggtgagc ttgtcaaagg gaactatgaa aattacagcg ccataagtat
     8041 tttggaggaa gaattcaata atgaatatgc cgattattta aaaggaatcc attttacctt
     8101 tacccttaaa ggaaacgaca acattacgga atttacagca tatatacaaa gagacaggtt
     8161 taggaaagag tggaattcgc tttctgataa gtccttaaaa aacttcatgg aaaaactatg
     8221 cagcgatata gccggggaat tcgaagagtg ctatatttac gggtacatat acgatacgga
     8281 cagcaatgag gaattggcat actgtgaaca gacagttgaa ggagatttcg tattcggcag
     8341 agaagattaa cagaaataaa gaatattata ggaggtcgct tatgaattac aatgaagtat
     8401 tggataaagc aaagtctcaa ataggatcaa aatgcagagt ttgccgtgaa tgcaacggag
     8461 tagtctgcag gggtgaaata ccgggagtgg gcggcaaggg cagcggaagc ggatttatta
     8521 ggagctatga gaagctcaat gagataaagc ttaatatgga tacaatatat gaatccaagg
     8581 aagtggatac atctatagag ctttttggca agagctttgc ttatccggtt tttgcagctc
     8641 ctatcggagg tttgcagcag cattacaggg aagattcaaa tgactatgaa tacagccatg
     8701 caattgtatc aggctgtaag aacgcgggta ccgcaggttt caccggcgac ggagtaaaag
     8761 atgagttcta cgaacagccg ttgaaggcta tatcagaagc tgggggctgg ggtataccga
     8821 cattaaagcc atggaagaat gatgaaattt tgagaaaaat taaaatggcg gaagcagcag
     8881 gggctttagc agcagctatg gatattgatg ccgcaggcct cgctcttctg gctgctttag
     8941 gcaaacccgt tagtccgaaa cctgttgaag aaattagaga aatagttgct ggtacgaggc
     9001 tgcccttcat aataaaggga attatgacag ctgcgggagc ggccaaggct gttgaagcgg
     9061 gggcgtatgg catagtggtt tccaaccatg ggggaagggt attggatcat actcccgcac
     9121 cggtggaggt attgccggaa atatacaagg cagtaggagg aaaaatcaaa atatttattg
     9181 acggcggaat aagaacagga attgatattg tgagggtact tgctcttggc gccgatgcag
     9241 tgcttatcgg aaggccttat gcaatagcag cctacggcgg cggtgcagag ggggttgagc
     9301 tttatacaag gaaaatcggt gctgagctta aagatgcaat gataatgaca ggctgcagca
     9361 cccttaaaga catagatgaa agagtgatat acaagccgta aaattgcgcc gtaaggcgct
     9421 ttttttatgc cttaagatgt attttattcc aacgggaatt taaagggctt cagaggaaaa
     9481 aataaaactg gcaccctgca gcataatcgc taattatgtg caaggcttcc cgaatagaat
     9541 taaacaggag gttatggata tggaaggggt gaaaaggctg gacggctggg ccaaattcaa
     9601 gggctttatc catttgctga ggccaaagca gtggataaaa aacctgtttc tgtttgcggc
     9661 actgctgttt tcaaggaaca tggacaaacc gcagtatgtt atgactgtct tatatgcctt
     9721 tctgtgcttc tgtatgatat cctccgcagt ttatgtattt aatgatatta gtgatgcgga
     9781 aaaagacagg cagcatccga gaaagagaat gcggcccata gctgcaggga taatagacag
     9841 aagggaagca caaatattga tgataatatt gcttcctgta tcagttatat tatccattat
     9901 gctggattac agctttggtt ttatagtcat gacatatttg cttaataatg tggtatatac
     9961 gctgtatgtt aaaaatattg ttattcttga tgttatgtcc attgccctgg gcttcatact
    10021 gagagtttca ggaggggcgg tagttattgg ggtgacaata tcaccctggc tgctgctgtg
    10081 cacctttctg ctgtcactgt ttctcggctt cagcaaaagg agaaatgagc tgctgatgct
    10141 tcaggcggat gcccataacc acaggaacaa tctggagcac tattccctgg attttattga
    10201 caacatgctt tcgattgtaa cggcatccac attgatatcc tatagccttt atacgtttta
    10261 tgccagcgaa ggcaagcatt caatggtgac catcctgttc gttctttatg gaattttcag
    10321 ataccaatat atcgtataca ataaaaaaat gggagaaagt ccggaggaaa taatactgac
    10381 cgacatacct ctgattgttg acataatgct atggatattg gtcagtataa taatacttta
    10441 t
//
LOCUS       DAOQUR010000076        10141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7425_length_10141_cov_11.470950, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000076 DAOQUR010000000
VERSION     DAOQUR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_10141_cov_11.470950"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..140)
                     /locus_tag="PKI50_11180"
     tRNA            complement(64..140)
                     /locus_tag="PKI50_11180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Arg,seq:cct)
     gene            complement(300..1118)
                     /gene="zupT"
                     /locus_tag="PKI50_11185"
     CDS             complement(300..1118)
                     /gene="zupT"
                     /locus_tag="PKI50_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNT04303.1"
                     /translation="MNSNAVLLAFGLTLIAGLSTGIGSLLAFFAKRTNTRLLSGVLGF
                     SAGVMIYVSLVDILPHANSILRASMGDRTGSWVSFISFFGGILLIAIIDKLIPSSENP
                     HESRRVEQMGQNYCDIKGNNMHLMKMGVLTAVAIGVHNFPEGIATFIAALKDPSLGIA
                     ITIAIAIHNIPEGIAVSVPIYCATGDRKKALVYSFMSGLSEPLGALLAYIFLMPILSD
                     QVFGIMFALVAGIMVFISLDELLPTAREYDEPHIATYGLVCGMALMGISILLFM"
     gene            1496..2860
                     /locus_tag="PKI50_11190"
     CDS             1496..2860
                     /locus_tag="PKI50_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT04304.1"
                     /translation="MIKNFILDTNVLVHDPYCISNFQDNNVIIPIMVVEELDSLKKRE
                     GMLGFHARSAAREINKLREYGSLSEGVCLPGGGTFRIELDHLESIELPDGLDLSKTDN
                     KILALTKNLQRENTGQPTILVTKDVYMAIKGDSLGITVQDYETDKVSVESIYTGYTEL
                     MLPSKDIEKIFKDGLKLPKQIDETIYPNEFIHVKSNDTSSNGVLTIFNGDRIVPLKYA
                     NNNAWGLVPINREQKMAFELLMDNNIHFVTLAGGAGSGKTILSTAVALQKVIEEEVYR
                     KIVFIKPVVPAGNDIGYLPGTEAEKLKPWMGSFYDAVDNLFGNRYRRKEKPSKSDAMA
                     KPTFTVDSFIEDYRQHGIIETKTFTFMRGRTLSDALVVVDEAQEITPHLAKMMLTRAG
                     FGAKFVFLGDPTDSQIDNVLVDARSNGLVYTIEKMKPFQITGHVTLNKVERSPLAQIA
                     EKYM"
     gene            2941..4365
                     /locus_tag="PKI50_11195"
     CDS             2941..4365
                     /locus_tag="PKI50_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT04305.1"
                     /translation="MNYKRLLELILLNLDEGIIVTDAKANITFYKEPVTNIGGIDSHT
                     AVGKNILDVFPDLTPETSTFYNVLRTQKPIIEHIQHYTNYQGKKVSTVTSTIPIIENG
                     ELTGCFEIFKDLTQVMELSEKIMHLQEKLYKSSKGENQFNENGAKYTFENIIGISSSI
                     RQLKSKAGRIAGSNSPVLVYGETGTGKELLVQAIHNSDPKRKNKPFIAQNCAALPFTL
                     LESILFGTAAGSFTGAKDNPGLFELAHGGTLYLDEVNSLNTELQAKLLRVIQDGVVRR
                     VGAAKTRDVNVRIIASTNEHPEELVKRKLLREDLYYRLNVIYLELPPLRERKEDIDAL
                     VKHFIERNNESLGKKVTGVSEEVMKVFEEYPWPGNVRELEHLVESTMNLTDKKVISLE
                     DVQHYFDNRLKIAAINSEKPEKDIPVKNLNTAVAKYEKELIIKALKASNGNISMAARN
                     LGLPKQTMHNKVKKHNIKLTAEIR"
     gene            4530..5411
                     /locus_tag="PKI50_11200"
     CDS             4530..5411
                     /locus_tag="PKI50_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNT04306.1"
                     /translation="MISGIVFNIQRYSIHDGPGIRTTVFLKGCPLSCIWCHNPESQSF
                     GPELMVYNKRCIGCGKCAEVCAHNAISDRNKCISCGSCTEVCHAKARELAGKKMSVRE
                     VIAQIEKDSIFYDESGGGVTFSGGEPLSQPEFLLELLKQCKKREYHTAVDTSGYGAAE
                     TIEAISGYTDLFLYDLKLPDDDKHIKYTGVSNKLILENLRLISRLGKKIFIRIPLIPG
                     INDDEEDIRATAEVIRGIAGVEQVNVLPYHNIAADKYARLGKHCSLKDIKVPSDGYME
                     HIAKKFFDYGVKVKIGG"
     gene            5416..7776
                     /locus_tag="PKI50_11205"
     CDS             5416..7776
                     /locus_tag="PKI50_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HNT04307.1"
                     /translation="MNDRVRMLRQRSLDAVPAISLERAQIVTGVYKKYYGKVSIPVLR
                     ALVFKELMENKTICINEGELIVGERGPKPKATPTYPELCCHTVGDLDVIDKRQKIFFR
                     VSEEAKDIQRNEIIPYWKGRPMRDLMFAQMTQEWHDCYESGIFTEFMEQRAPGHTVCD
                     DKIYKKGFIGFKEDIEKQLAALDFVNDMDACNKQEELRAMSICADAIISFGLRHAEKA
                     LELALRESNPERKEELKQIADVCYNVPANAPGSFHEALQMYWFVHLCVITELNTWDAY
                     CPGRLDQHLYPFYKKGIDDGTLTPEKAEELLQCFWIKFNNQPAPPKVGITLQESGTYT
                     DFANINSGGLKADGSDGVNDVTYMVLDVIDEMRLLQPSSNIQLSKKNPDRFLKRACRI
                     ISKGWGQPSVFNADSVVEELVRQGKRIEDARNGGTSGCVETGAFGKEAYILTGYFNLV
                     KVLEITLNNGIDPRTGKKIGLETGDPREFKNYGELMDAYKKQLHHFIEIKIRGNNVIE
                     KLYADLMPAPFLSILTDDCIKNGKDYNAGGARYNTNYIQGVGIGTLTDSFSAVKKHVF
                     DDKSLTMDELLDALKNNFEGREAMRQLIMNNTPKYGNDDDYADEIMKELFDAYYNEVN
                     GRRTVKGGSYRINMLPTTCHVYFGSVTGATPDGRRSGQPQSEGISPVQGADMYGPTAV
                     IKSAGKMDHIKTGGTLLNLKFTPDLVKDDEGIDKLSQLVRSYFKLDGHHIQFNVVSAD
                     TLRKAQKNPEQYKDLIVRVAGYSDYFCDLTEALQEEIITRTEHKSL"
     gene            7798..9582
                     /locus_tag="PKI50_11210"
     CDS             7798..9582
                     /locus_tag="PKI50_11210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNT04308.1"
                     /translation="MTVNEKISRLRELMKGKNIDAFIIPSFDAHQSEYVPEHWMARQW
                     ISGFTGSAGTAVVTMKEAGLWTDGRYFIQAENQLKESEIKLFKMGQPDVPDYVQWIKE
                     VLPVGGTVGFNGKSMPVSLYMDMTKAYKPRKLTIEAGYDLIGELWIDRPAMPKAPIFI
                     HDTVYAGKSRAEKLTDLRTEMEKLNSEYYLISALEDIAWLLNIRGGDVPNTPVVSAYV
                     LVSRTQCRLFIDESKLNADVRKELEADSIEIHNYESIQKAVASLDAGASIIIDPEKTN
                     VWLYKSIPGAVEILEMPNITTNMKAIKNSIEIQNLRNAHIKDGIALVKFLIWLDSSLG
                     KNKITEITVEEKLKEFRLQQELFIGPSFDTIAGYKDHGAIMHYKAVPGIEYALEKEGM
                     LLIDSGGQYLDGTTDTTRTIVLGELTQEQRKDFTLVLKSHIGLATARFLHGCTCTHLD
                     ILARKPIWDEGLDYRCGTGHGVGFLLSVHEGPQGFRRTPIINSIVLQPGMILTNEPGI
                     YKEGKHGIRTENTLLVTDDFENEYGRYLKFDTISFCPIDLEGLDISLLTQQEKDWLNN
                     YHREVYNKLSPYLNEKEKAWLKNETRAV"
     gene            complement(9626..10090)
                     /locus_tag="PKI50_11215"
     CDS             complement(9626..10090)
                     /locus_tag="PKI50_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HNT04309.1"
                     /translation="MNKSRYLVHAASIAAVYAVLTIAFAPLSYGQIQVRFAEALTILP
                     FFTSAAIPGLFVGCIVANFFGPGGIPDVVFGSAATLIAAYISYKMPAKWLVPLPPVIA
                     NGLIIGYLLHYLYQLPLLITIGWVALGELISCYFIGYPLLLILEKYRDKIFK"
BASE COUNT         3239 a         2096 c         2320 g         2486 t
ORIGIN      
        1 gattgcatcc aaaacttcaa aaaataaaaa actgtctatt aattagacag ttattctcat
       61 atatggtgca cctaaaggga ttcgaacccc cgacctgcgg tttaggaaac cgtcgctcta
      121 tcctgctgag ctataggtgc acagtcgact cacattaaaa tagtatataa aaatcgttgc
      181 tttgtcaatc gtaaattttg gcactatgct gtatttaggc cttctacagc aatatagttg
      241 cttaatatga tgtcttttat gcacccggtg cttttttatg ccggtttcca aaaaattttt
      301 tacataaaaa gcaatatgct tattcccata agagccattc cgcataccaa tccatatgta
      361 gctatatgcg gttcgtcata ttctcttgca gtaggaagaa gttcatccag ggatataaaa
      421 accataatgc ctgcaaccag tgcaaacatt atcccaaaaa cttgatcgct taatatcggc
      481 atcaaaaata tatatgcaag caacgctccc aatggctctg ataaacctga cataaaagaa
      541 taaacaagtg ctttttttct gtctccggta gcacagtaaa ttggtacgga tacggcaata
      601 ccttcaggta tattgtgtat tgctattgca attgtaattg caatacccaa gctaggatcc
      661 ttaagggctg caataaaggt tgcaatccct tccgggaagt tatgtactcc gatagccacg
      721 gctgtaagca cacccatttt catgagatgc atattattgc cctttatatc gcaatagttt
      781 tgtcccattt gttccactct tctgctctcg tgcggattct cagaggaggg aataagctta
      841 tcaattatag ctatcagcaa tattcctcca aaaaaggaaa taaatgaaac ccaacttcct
      901 gtcctgtctc ccatggacgc tctaagaata ctgttggcat ggggaagtat atctaccaaa
      961 gatacatata tcattacacc tgccgaaaac cccagaactc cggacagcag ccttgtattt
     1021 gtccgctttg caaaaaatgc caaaaggctt cctattccgg tagataatcc tgcgattaag
     1081 gtgagtccaa aggctaaaag tacagcatta ctgttcatat attattcctc tcctgcgaaa
     1141 aatcaataat aaatagtatg tatccccttc tattatattg ataatggttc ccatttacaa
     1201 tacattccta taatttttct aaatgtactt gaaaaaaatt tcttgaatgt taataaaatt
     1261 ctgcttgcca atggatatat atgataaata agttttcaga atgtatttgt tcagcataaa
     1321 ataataatat aacaatcaga aatcttatta taatagccct tgtgaatttt atgtaaattt
     1381 tctcgacgat tgcaggaatt taatgatatt tgacagaatt tatattatac ataatcactc
     1441 ctaaagcgga gccatatacc aggaagcata tatcatctca tatggaggta agtgcatgat
     1501 aaagaacttt attcttgaca caaatgtgct ggtccatgat ccttattgta tctccaattt
     1561 ccaggataac aatgtgataa tccccatcat ggtagttgaa gaactggata gtcttaaaaa
     1621 aagggagggt atgttgggtt ttcacgcaag aagtgctgca agagaaataa acaagcttcg
     1681 tgaatatggc agtttaagcg aaggagtttg tttacccgga ggaggcacct tcagaattga
     1741 attggaccat ttggagtcca ttgagctgcc ggatggtttg gacctttcaa agacagataa
     1801 taagatactt gcactgacta agaatcttca aagggaaaat accgggcaac cgaccatact
     1861 tgtgacaaaa gacgtttata tggcaataaa gggtgacagc cttggaatca ctgtacagga
     1921 ctatgaaact gacaaggttt ctgttgaatc catatatacc ggctataccg agctgatgct
     1981 tccctcaaaa gatattgaga agattttcaa ggatgggttg aagctgccta aacagattga
     2041 cgaaaccatt tatcccaacg agttcattca tgtaaaaagc aatgacactt ccagcaatgg
     2101 agttcttaca attttcaacg gagacaggat agtcccgctg aagtatgcaa acaataacgc
     2161 ctggggcctt gttcctatca acagggaaca gaaaatggcc tttgaactgc ttatggacaa
     2221 taatattcat tttgttacat tggccggagg cgcaggttcc ggaaaaacaa tattatccac
     2281 tgcagtagca cttcagaagg ttattgaaga agaggtttac cgaaaaatcg tatttataaa
     2341 gcctgttgtg ccggccggca atgacatagg ctacctgccg ggcaccgaag cggaaaagct
     2401 gaagccctgg atgggctcct tctacgatgc cgtagacaat cttttcggaa acaggtacag
     2461 gcgcaaggaa aagccaagca agtcagacgc catggccaag cccaccttta ccgtggattc
     2521 cttcatcgag gactacaggc agcacggaat tattgagacc aagactttta cctttatgag
     2581 gggaaggacg ttgtccgatg cccttgtagt tgtagatgaa gcacaggaaa tcactcccca
     2641 tcttgccaaa atgatgctga cccgtgcagg cttcggagcc aaattcgttt tcctgggtga
     2701 tcctacggac agtcagatag acaatgtcct ggtggatgca cgttcaaacg ggcttgtgta
     2761 cactatagaa aaaatgaagc ctttccagat caccggacat gtaaccctta acaaggtgga
     2821 acgaagccct ctggctcaga tagcagaaaa atacatgtag gtgcaatgaa ctttggactc
     2881 ttgattagca ctcaagagtc ctctattata aaactaataa tacttttgtg agggattgcc
     2941 atgaactaca aaagactgct ggagcttata cttcttaatc ttgacgaagg aataatcgtc
     3001 actgacgcaa aggccaacat tactttctat aaggagcctg ttaccaacat aggaggtatt
     3061 gactcccata cggctgtcgg aaagaatata cttgatgtct tccccgatct taccccggag
     3121 acaagcacct tctacaatgt actgagaact caaaagccaa taatagaaca catacagcat
     3181 tatacaaact accaggggaa aaaggtttct accgttacct ccaccattcc cataatagaa
     3241 aacggggaat taacgggttg ctttgaaata ttcaaagatc ttacccaggt tatggagctg
     3301 tccgaaaaaa taatgcatct ccaggagaag ttatataaaa gcagcaaagg ggaaaaccaa
     3361 ttcaatgaaa acggagcaaa atacaccttt gaaaatataa ttggaataag ttcctccatt
     3421 cggcagctaa aatcaaaagc cgggcggata gccggaagca attccccggt gctggtgtac
     3481 ggagaaacag gtacaggcaa ggaacttctg gttcaggcaa tccataattc ggaccccaaa
     3541 agaaaaaata agccctttat cgcgcaaaac tgtgcagcgc ttcctttcac tctccttgaa
     3601 tcaatacttt tcggtacggc tgccggcagc ttcaccgggg caaaggacaa tccaggactt
     3661 tttgagcttg ctcacggcgg cacactttac ctggatgagg taaattccct taatacagag
     3721 ctgcaggcca agcttctcag agtcatacag gatggagttg taagaagagt tggggcagcg
     3781 aaaaccaggg atgtaaatgt caggataatt gcatcgacaa acgagcaccc ggaggagctg
     3841 gtaaaaagga agctcttgcg agaggacctc tattacaggc tcaatgtcat atatcttgaa
     3901 ttgccccctc tcagggaaag aaaggaagac attgatgcat tggtaaagca ttttatcgag
     3961 cgcaacaatg aaagtctggg gaaaaaagtt acaggcgttt ccgaagaagt catgaaggtt
     4021 tttgaagaat acccatggcc gggaaatgtc agagagctgg aacaccttgt ggaaagcacc
     4081 atgaatctta ccgataaaaa ggtcatttcc cttgaagatg tgcagcatta tttcgacaac
     4141 aggcttaaga tagcagccat aaactcagaa aagcccgaaa aagatatacc tgtaaaaaat
     4201 ttaaataccg cggtagccaa atatgaaaag gaacttataa ttaaggcgct taaagcttcc
     4261 aacgggaata tctcaatggc tgcaaggaat cttggcctgc caaagcagac aatgcataac
     4321 aaggtaaaaa agcataatat aaagctgacg gcagaaataa ggtagtacca tttatggtac
     4381 tacgttccga atatgatact tattcgtacc aattttggta cgttgttttt ttgtattttc
     4441 gagaagccaa tagttgcaag ctcttcggat atatatgata tggcatgtaa tttgcatagc
     4501 ttgtatagta tacaatcggg ggtgatatta tcatatcagg gatagtattc aatattcaac
     4561 ggtattcaat acatgacggg ccgggaatcc ggacaaccgt ttttttaaag ggctgccccc
     4621 tttcctgcat ttggtgccac aaccccgaaa gccaatcctt cgggcctgag cttatggtct
     4681 ataacaaaag atgcataggc tgcggaaaat gtgcggaggt ttgcgctcac aacgccatat
     4741 cagacaggaa taagtgcatt tcctgcggaa gctgcacaga agtgtgccat gcaaaggcaa
     4801 gagaattggc gggcaaaaag atgtccgtcc gggaggttat tgcacaaatc gagaaggata
     4861 gtatttttta cgatgaatca ggcggcggcg tcactttttc cggcggtgag cccctgtcac
     4921 agccggaatt tttgctggag cttctgaaac agtgcaaaaa aagggagtac catacggcag
     4981 tggacacaag cggatacgga gccgcagaga caatagaggc aataagcggc tatacagacc
     5041 ttttccttta tgatcttaag ctcccggatg atgacaagca catcaaatat accggcgtat
     5101 ccaacaagct gatacttgaa aaccttaggc tgatttcaag gctggggaag aaaattttta
     5161 tcaggatacc gcttataccc ggaatcaatg atgatgaaga ggatataagg gcaacagcag
     5221 aggtgatacg gggtattgca ggcgtagagc aggttaatgt tctgccctat cataatattg
     5281 cagcggacaa atatgccaga ttgggaaagc actgcagtct taaggacatt aaggtgcctt
     5341 ccgatggata tatggaacat atagcaaaaa agttttttga ttatggtgta aaagtaaaga
     5401 taggaggttg aagctatgaa cgacagagtc aggatgctga ggcagagaag ccttgatgct
     5461 gtccctgcca tatccctgga aagagcgcaa attgtaaccg gtgtctacaa aaagtattat
     5521 ggaaaggtat ccattcctgt cttaagggca ctggtcttta aggaattgat ggaaaacaaa
     5581 actatctgca taaatgaagg agagcttata gtaggcgaaa gagggcctaa accgaaagcc
     5641 acccccacat atccggagct ttgctgtcat actgtcgggg acctggatgt aatagacaaa
     5701 aggcagaaga tatttttcag ggtcagcgag gaagcaaagg atatacagcg gaatgaaata
     5761 ataccctatt ggaagggcag gcccatgcgt gacctgatgt ttgcacaaat gacgcaggag
     5821 tggcacgact gctatgaaag cgggatcttc acagagttta tggaacagag ggctcccggc
     5881 catacagtgt gcgatgataa aatatataaa aaaggcttta taggctttaa ggaggatata
     5941 gaaaaacagc ttgcagccct tgactttgtt aatgatatgg atgcctgtaa caagcaggag
     6001 gagcttaggg caatgagcat ctgcgccgat gcaataataa gctttggact gcgccatgcc
     6061 gagaaagccc tggaactggc cctgagggag agtaatccgg aaagaaagga agagcttaag
     6121 cagattgcgg atgtctgtta taatgtaccc gcaaatgctc ccggaagctt ccatgaagct
     6181 ctgcagatgt actggttcgt acatttgtgt gtaatcaccg agctaaacac ctgggatgca
     6241 tactgcccgg gaaggctgga ccagcacctt taccccttct acaaaaaggg tattgacgat
     6301 ggtactctta cacctgaaaa agcggaggaa ttgctgcagt gtttttggat taaattcaac
     6361 aaccagcctg cccccccgaa ggtaggcata acccttcagg aaagcggaac ctataccgac
     6421 tttgccaaca taaacagcgg gggccttaag gccgacggtt ctgacggagt aaacgacgta
     6481 acatacatgg tgctggacgt aattgacgaa atgaggctgt tgcagcccag ttcaaacata
     6541 cagctgagca aaaaaaaccc cgaccgtttc ctgaaaagag catgcaggat aataagcaaa
     6601 ggctggggac agccttcagt attcaatgcc gactcggtgg tggaggagct tgtacgccag
     6661 ggcaagcgca ttgaagatgc cagaaacggc ggaaccagcg gatgtgttga aacaggtgcc
     6721 ttcggcaagg aagcttatat actgacaggt tacttcaacc tggtaaaggt gcttgaaatc
     6781 actttaaata acggtatcga ccccagaaca gggaaaaaaa tcggattgga gaccggggac
     6841 cccagagaat ttaaaaacta tggcgagctt atggatgctt acaagaaaca gctgcatcat
     6901 tttattgaga taaaaatcag agggaataat gtaatagaaa agctttatgc cgaccttatg
     6961 cccgctccct tcctttccat tcttaccgat gactgcataa aaaacggaaa ggattataac
     7021 gcaggaggtg ccagatataa cacaaactac atccagggag taggaatcgg cactttgaca
     7081 gacagctttt cagccgtaaa aaagcatgtg tttgatgata aatccttaac gatggatgag
     7141 cttctcgatg cgcttaagaa taactttgaa ggcagggagg ccatgcgcca gcttataatg
     7201 aacaacactc ccaagtatgg caatgacgat gattatgccg atgaaataat gaaggaactc
     7261 tttgatgcct attacaatga ggtgaacgga agaagaacag taaaaggagg aagctacagg
     7321 ataaatatgc tgcccaccac ctgccatgta tatttcggct ctgtcacagg tgcgacccct
     7381 gacggaagac ggtcgggaca gccgcagtcg gagggtattt ctcccgtaca aggtgccgat
     7441 atgtatgggc cgacagcagt aataaagtcg gcgggtaaaa tggaccatat caaaaccggc
     7501 ggtactctcc tgaacttaaa attcactcct gatctagtaa aggacgatga gggcattgat
     7561 aagctttcac agcttgtaag gagctatttc aagctggacg gccatcatat acagttcaat
     7621 gtagtaagtg ccgacactct aaggaaagca cagaagaatc cggaacaata caaagacctt
     7681 atagtaagag ttgccggcta cagtgattat ttctgtgact tgacagaagc cctgcaggaa
     7741 gagataatta caagaacaga acataaaagc ttatagatga aaggggttaa cgaaaaattg
     7801 acagtaaacg aaaagatctc ccggctcaga gaacttatga agggaaaaaa tatcgatgcc
     7861 tttattattc cgagcttcga tgcacatcag agcgagtatg tgccggagca ttggatggcc
     7921 aggcaatgga tatccggctt taccggctct gccggcacag cagtcgttac gatgaaagaa
     7981 gcaggcctat ggaccgacgg aaggtatttc attcaagccg agaatcagct taaggaatct
     8041 gaaataaagc ttttcaaaat gggtcagccc gatgtgcctg actatgtcca atggataaag
     8101 gaagtgctcc ctgttggagg aacagtgggc tttaacggca agtccatgcc tgtatccctc
     8161 tatatggata tgacaaaagc atacaaaccc aggaagctta ccattgaagc aggctatgac
     8221 cttatagggg aactttggat tgacaggcct gcaatgccca aggctccgat ttttatacat
     8281 gatactgtat atgccgggaa atcaagagca gaaaagttga ctgacctccg tacagaaatg
     8341 gagaagctta attccgaata ttacctgata tctgctttgg aggatattgc atggctttta
     8401 aacatcagag gcggagatgt acccaataca ccggtggttt cagcttatgt gctggtttcc
     8461 cgaacccaat gccggctttt tatcgatgaa agcaagttaa acgctgatgt aagaaaagag
     8521 cttgaagcag atagtattga aatacataac tacgaatcaa tacaaaaagc cgttgcctct
     8581 cttgatgccg gagcttcaat aataatagac ccggaaaaga caaatgtgtg gctgtataaa
     8641 tcaatacccg gagctgttga aatattggaa atgccaaata tcacaacgaa tatgaaggcc
     8701 ataaagaaca gcatagaaat ccaaaatctc agaaacgctc atataaaaga tgggattgcg
     8761 ctggtgaaat tcctcatatg gcttgacagc agcctgggga aaaataagat tacggaaatc
     8821 accgttgaag aaaagctgaa ggaattcagg cttcagcagg agttgtttat tggccccagc
     8881 tttgatacca ttgctggcta taaggatcat ggggcaataa tgcactataa ggccgttccc
     8941 ggaattgagt acgctttgga aaaggaaggg atgctgctta ttgactcggg agggcaatac
     9001 cttgacggca caaccgatac cacaaggaca atagtgctgg gagaattgac acaggagcag
     9061 agaaaggact tcactctggt actcaaatcc cacatagggc tggcaacagc ccggttcctc
     9121 cacggctgca cctgcaccca tcttgatata ttggcaagga aaccgatatg ggatgaaggc
     9181 ttggactacc gctgcggcac aggccacgga gtcggattcc ttctcagtgt ccatgaaggc
     9241 ccacaaggct tcagaagaac tccgataata aacagtattg tacttcagcc gggtatgata
     9301 ctgaccaacg aacccggaat atataaggag ggcaagcatg ggatccgcac cgagaacacc
     9361 ctgcttgtaa cggatgactt tgaaaatgaa tacggcagat acttaaaatt cgatactatc
     9421 tccttctgcc ccatagacct cgaggggctg gatataagcc ttctgacgca gcaggagaag
     9481 gattggctca acaattacca tagagaagtg tataacaagc tgtctcctta tcttaatgaa
     9541 aaagagaagg catggctcaa aaacgaaacc agagcggttt agatggacaa aaataaaaaa
     9601 gcttatcaaa tccgataagc tttttttact tgaatatttt atccctgtat ttctcaagaa
     9661 tgagcagcag cggatatcct atgaaatagc aggatatcaa ttcccccaat gccacccagc
     9721 caatagttat cagcaaaggc agctgataca gataatgcag caggtatccg attataagtc
     9781 cgtttgcaat cacaggcggc agcggaacca gccactttgc cggcattttg tatgaaatat
     9841 atgcggcaat aagagttgct gcactcccga atactacatc aggtataccc ccggggccga
     9901 aaaagtttgc cactatgcag ccaacaaaaa gacccggtat ggcagcagaa gtaaagaagg
     9961 gcaaaatggt cagggcttct gcaaacctga cctgtatctg cccatagcta aggggcgcaa
    10021 atgctatggt gagtaccgca tatacggctg caatggacgc tgcatgtacc aggtatctgc
    10081 ttttattcat tttttaatcc tccgtagttt tgttttaagt caggatggtc tcgaactgac
    10141 t
//
LOCUS       DAOQUR010000077         9808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7749_length_9808_cov_9.121399, whole genome shotgun sequence.
ACCESSION   DAOQUR010000077 DAOQUR010000000
VERSION     DAOQUR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7749_length_9808_cov_9.121399"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2417
                     /locus_tag="PKI50_11220"
     CDS             <1..2417
                     /locus_tag="PKI50_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT04310.1"
                     /translation="GFIVSAKALLDQNVNPTRDEVREWFQKNRNVCRCTGYIPLVDAV
                     MDAARVLRGEIKMEDLWCKLEEGASMLGSKEPRPSALAKVTGTWDFGADLGLKLPENT
                     LHIKLVQATVSHANILSIDTSEAEKMPGVFKVITHKDVPGTNRINGLAFPQNKGDGKE
                     RPILNDKKVFQFGDALAMVLADTPTQAEEAAKKVKVELEVLPAYMSAPEAMAEDAIEI
                     HPGTPNIYFETHRIKGEDTKPLMESLPYVIEDDFYVGRQPHLVLEPDVGFAYLNEEGK
                     LIVHSKSIGLHLHHLMTAEGIGIDPDKYILVQNPAGGTFGYKFSPTNEALLGVAALVS
                     KRPVFLEFNMYQQITYTGKRSPFFIHLKYGADKDGKLKAMEADWSVDHGPYCEFGDLV
                     TMRGTQFIGAGYDIPNSRGVGRTVATNHAWGSAFRAYGSPQSLFASEVLADMMAERIG
                     MDPLEFRYLNVYREGSTTPTGCAPDVIAFPQAIDTLRPLYKEAKERAKGKNKNGGNIK
                     YGVGISLNMYGCGLDGADSSEIWVELTPEGVTVGTSWEDHGQGADMGTLTYTYEALRP
                     LGIKPENIKLVMNDTEKTPNSGPAGGSRQNVVTGNAIVAGCRKLLEAMKKPDGTFRTY
                     DEMVAEKLPLRYEGKWTAPKTACDVDTGQGDPFSTYMYGVLMSEVAVDTTTGKTQVEK
                     MTIVSDCGTIVNKLVLEGQIYGGLVQGIGLALSEDFEDLKRHTTLTGCGIPQILDTPD
                     NIEIIHQETPRPYGPYGASGVGEMPLSAPHASIINAIYDACGVRIKHLPAYPEKVLAG
                     LKELNK"
     gene            2502..4772
                     /locus_tag="PKI50_11225"
     CDS             2502..4772
                     /locus_tag="PKI50_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT04311.1"
                     /translation="MDLDKLLETSDSCIHDEPPACTSCCPVHMDVIAFVGEMEKGDFD
                     KAYKVMEKRVPFARIIGSICDHPCEKVCVRSDSGGTVKISELEKAAVSYGFSPPKNTI
                     PLSKSKGRVAVIGGGISGMTAAFELDKKGYQVTLYEKSDRLGGRIWDYEGIALKKEVI
                     EEEIAALVKQGIKAELNTLINKKRLEELAGEYDAVYLGTGVWEEELKIHPETFQVGDT
                     SVFAGGKLANNNDSVIFSASSGRRAAASVDRYVKKISLSASREREGSFETPLKYETEG
                     IEPVEAIGKTSDIYSEDEAIGEAGRCLKCRCRKCIESCSHLTRFNISPKSYMRQINQN
                     ERIILGTHYANKMINSCTMCGLCREECFKGISMKAVIKETRESMVRRGKMPVSDHDFA
                     LKDMKFSNSKYFSMVRNQGEKAEYLFYPGCQLPASYPEYIGKIYKYLVSNMSGGAGIY
                     LGCCGAPADWAGRQDLMQENIEIIKKTWEDTGRPVFILACSSCCSVFEKYLPEIAFVS
                     LWEIFARKGLPQNNRIPGKRVLSVHDACATRHNKEIHDSIRRIAVLSGYEIQELKYSK
                     EKTKCCGYGGLVYFANREQSADFVKHRIEESDNDLLVYCAMCRDLLAEGGKRTFHILD
                     LIFAEDMERAALRKMPTLSERHGNRAQVKIGLLREIWGEEPDMDYEKQRDFKLIIPEN
                     VRQQMEDRYILSEDMAEVIDSSRKSGERFFNPQENSCLARLRLDNVTYWVKYEEKDEK
                     ILVKSVYSHRMEIVEE"
     gene            4775..4981
                     /locus_tag="PKI50_11230"
     CDS             4775..4981
                     /locus_tag="PKI50_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNT04312.1"
                     /translation="MNEDKNYNMPWLCDKCKRELVLKKVKVRYLEGNFEVDLPKCPEC
                     NMVFIDEEMAVGKMLEVEKELEGK"
     gene            5002..5451
                     /locus_tag="PKI50_11235"
     CDS             5002..5451
                     /locus_tag="PKI50_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNT04313.1"
                     /translation="MNDIAFKLLKLSSSGFCCTQIMLKLALDEEGKENEDLLKAVSGL
                     CRGIGGRQKSCGVLTGGLGILGLYAGKGKDTEYPKQNYSTMTEQYLEWFESEFGSEEC
                     KDIIGVYDFFDDTGNSYMIKCGDILLKSYEKVVEILRDNDYEYGSRE"
     gene            5454..6764
                     /locus_tag="PKI50_11240"
     CDS             5454..6764
                     /locus_tag="PKI50_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT04314.1"
                     /translation="MKQTPIEQWIMQKTGIKERDKKALEAYQLGKIKETIAYVKENSI
                     FYCKQLKNIREESINSPESFQNIPFTYPRDIKRNPPDFLCVPQRKVARIVTLKTSGTS
                     GEDKRLFFTEEDIELTIDFFRHGMSCLVDKSDKVLVLMPGSTYGSIGDLLKKALALSD
                     IECIVHGVLTDPDEAARLIEEKGITCIVGIPLQVLYLSRVKSNVFKNSIKKVLLSTDY
                     VPDVLTNELERRFGCRVFDHYGMTEMGYGGGVECEALNGYHMREGDMYFEIIDPDTGK
                     AVECGQYGEVVFTTLTRQAMPLIRYRTGDIASFSSNPCACGTFLNTMKKVLGRIENRV
                     RIGEERFLYLRELDEIILPLEEVLDYRAYLDDKDRLVLEVTVKDGIDFTEIKEEVMRS
                     IRNLLSNQSKCGLSVEIVKAAVKEPASITDSMVKRKIIDYRKER"
     gene            6768..7826
                     /locus_tag="PKI50_11245"
     CDS             6768..7826
                     /locus_tag="PKI50_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNT04315.1"
                     /translation="MKDIYELINEQLDKKESFVLATILKKLGSAPREEGTKMLIRKDF
                     SIEGTIGGGISEAMTIKLSAEVFEDGLYIIENFLMSNKDSACLGMVCGGDISVLLEYV
                     DCNDSQMVDIYRKAIELKHCGTDFVMITKLSEDRRKIRGSDKWICTETGFYGMENEEI
                     WETIKNIRVNFKETKIREIFIGKERYIIEPFFNVESVYIFGAGHVAQKISDITKMLGF
                     YTVVMDDREEFANRERFETADEVRVIQSFNNLENYDIINGNSYIVIVTRGHVYDKDVL
                     AQMLRTDARYIGMIGSRSKREHVYNNLLSEGFTSEDLERVHCPIGLNIHAQTPEEIAV
                     SIAAELIKVRREPKDKTH"
     gene            7866..9752
                     /locus_tag="PKI50_11250"
     CDS             7866..9752
                     /locus_tag="PKI50_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT04316.1"
                     /translation="MNNIDYKHVIELSHERCRKFNIKEDQVYSSRIINETELQKRFAE
                     NRSMILTAAPYMEQLINFVKGHNFFALLTDGEGCILNAIGDEKILSEAFDLKMIPGAF
                     MNEESIGTNAMGIVIKTGEPVQLSGKDHFVKAYHRWTCSGAPIKDHNGNLIGVLDLTG
                     YTECVHPHTLGMVIAASNAIEEMIKVKEFNNLQDINNKHIKTIFNTMPVAIVTSDMYG
                     KIKIYNNKALELLDNKDNQLKAKYMSEIIEEWDRIKQDIYQGRNISQEVNIIVFRNRL
                     SCRLIANPIYNSVDDSVEIVYALEEIKKEKRDKKKSEYQAYYTFDKIIGKDENFLKIV
                     EYAKKISDSKSTILIMGESGTGKEVFAQAIHNFSKRMDGPYLALNCGAIPNQLIESEL
                     FGYEEGAYTGAKKGGNVGKFELASGGTIMLDEIGEMPLDMQTKLLRVLQEGIITRIGS
                     AKPIPVDVRVIASTNKDLKKEVQLGRFRKDLFYRLNVLPIFLPALSQRKGDILLLTDY
                     FMKKIAEKLGKKEVKIPKGYIKSMMEYDWPGNVRELENFIELIIYSESIPYDYFNQEK
                     SFAEELYDNYIQGSDMNLCNMEKAHLKKVLKKHNGNITRSAESLGISRNTLYSKMKKY
                     RVKV"
BASE COUNT         3229 a         1655 c         2512 g         2412 t
ORIGIN      
        1 cgggattcat tgtgtccgca aaagctttac ttgaccagaa tgtaaatccc acaagagatg
       61 aagtaagaga atggttccaa aaaaacagaa atgtatgcag atgtacagga tacattccgt
      121 tggtcgatgc agttatggac gctgcaaggg ttcttagagg cgaaataaaa atggaggatc
      181 tgtggtgcaa actggaggaa ggcgccagca tgctgggttc caaagagccg cgcccttctg
      241 ctctcgcaaa ggtgacagga acatgggatt ttggagcgga tttaggctta aagctgcccg
      301 aaaacacatt acatatcaaa ttggttcagg cgacggtttc ccatgccaat atactttcca
      361 tagacacctc cgaagcagaa aaaatgcccg gtgttttcaa ggtaattacc cataaggatg
      421 tcccgggaac aaacaggatt aatggcctgg catttccgca gaataaggga gacggaaagg
      481 aaaggccgat acttaatgac aagaaggtgt tccaattcgg agatgcactc gctatggtgc
      541 ttgcagatac tccgactcag gcagaggaag cagctaaaaa ggtaaaggtt gagctggaag
      601 tattacccgc ttatatgagc gcacctgaag caatggctga ggatgctata gaaatccatc
      661 ccggtacgcc caacatttat tttgagacac acagaataaa gggggaggat accaaacctt
      721 taatggaaag cctgccctat gtcattgagg atgactttta tgtaggaagg cagccccacc
      781 tggttctgga gccggatgta ggctttgctt atctgaatga ggaaggcaag ctgattgtcc
      841 attcaaagag tattggtttg cacctccatc atttgatgac ggctgaaggc ataggaattg
      901 accctgataa gtatatactt gtacagaatc ctgcaggagg gaccttcgga tataaattca
      961 gccccaccaa tgaggccttg ctgggtgtgg ccgcactggt atcaaagagg ccggtattcc
     1021 ttgaattcaa tatgtaccag cagattactt ataccggaaa gagatctcct ttctttatcc
     1081 atctcaagta tggagcagac aaagacggta agctgaaggc aatggaagcg gactggtcgg
     1141 ttgaccacgg cccatattgc gagtttggtg atttagttac catgagaggt acccaattca
     1201 tcggcgccgg ttatgatata ccgaacagca gaggagtagg aagaactgtt gcaactaacc
     1261 acgcatgggg ttccgctttc agggcttacg gttcacctca aagcctgttt gcatctgaag
     1321 tgctggcgga catgatggcc gaaaggattg gtatggatcc tctggaattc agatatttaa
     1381 acgtatacag agaaggctcc actacaccaa caggctgtgc accggatgtt attgcattcc
     1441 ctcaggctat agatactctc aggccactat ataaagaggc aaaagagaga gccaaaggaa
     1501 agaataagaa tggcggaaac atcaaatacg gcgtaggcat ttcccttaat atgtatggct
     1561 gcggccttga cggcgcggac tcttcggaga tctgggtaga gcttacccct gaaggtgtta
     1621 ctgtaggtac ttcatgggag gaccatgggc agggagccga catggggaca ctgacatata
     1681 cttacgaagc attaaggccg ttagggataa agcccgagaa tataaaattg gttatgaacg
     1741 atacggagaa aactcccaat agcggacctg ccggcggaag ccgccaaaat gtagttacag
     1801 gcaatgcaat agtagccggc tgcagaaagc tgttggaagc catgaaaaag ccggacggta
     1861 cattcaggac ctatgatgaa atggtcgcag agaagctgcc tctcagatac gaaggcaaat
     1921 ggactgcacc gaaaaccgca tgtgatgttg acacaggaca gggagatccc ttctccacct
     1981 acatgtacgg cgtgctgatg agtgaagtgg cagtggatac aactaccggc aaaactcaag
     2041 tagagaaaat gactattgta tcagactgcg gcacaattgt aaataaactg gtattggaag
     2101 ggcagattta cggcggactt gtacaaggga taggcctggc attgagcgag gattttgaag
     2161 acctgaaaag gcatacaact cttacaggct gcggaattcc tcaaatcttg gatactcccg
     2221 acaatattga gattatacat caggaaacac caaggcctta tggaccgtac ggagcttccg
     2281 gtgttggaga aatgcccctt tctgcgcccc acgcttcgat aatcaatgca atatatgatg
     2341 cctgcggtgt acgtataaag catctgcctg catatcctga aaaggtcttg gctggcttga
     2401 aggagttaaa taaataattt tgattgtaaa attcatgaaa gccggaatat ggaattggga
     2461 ttttattccc aattcctatt ttggcaaagg aggtacaaac aatggatctg gataagctgc
     2521 ttgaaaccag cgacagttgt atacatgacg aaccacccgc atgtacctca tgctgtcctg
     2581 ttcacatgga tgttattgct tttgtcggtg aaatggagaa aggggatttt gacaaagctt
     2641 ataaagtaat ggaaaaacgg gttccttttg caagaataat aggaagcata tgtgaccatc
     2701 cttgtgaaaa ggtttgtgtc agatcggatt ccggaggaac ggtcaaaata tcagaattgg
     2761 agaaagcagc tgttagttat ggcttttctc cgccaaagaa taccattccc ctgtctaaaa
     2821 gcaaggggag agtggcagtt atcggcggag gaatcagcgg catgacggca gcctttgagc
     2881 ttgacaaaaa gggttatcag gttactttgt atgagaaatc agacagattg ggcggcagga
     2941 tatgggatta tgaagggatt gccctcaaaa aggaagtaat agaggaagaa atagcagcct
     3001 tagtgaaaca ggggattaag gcagagttaa atacccttat taataaaaaa aggctggaag
     3061 agcttgccgg agaatatgat gcagtatacc tgggaacagg tgtatgggag gaggaactta
     3121 aaatccatcc tgagaccttt caggtaggag atacttcggt gttcgcggga ggaaagctgg
     3181 caaacaacaa tgattcggta atattttcgg caagttcggg cagaagggct gccgcatccg
     3241 ttgacagata tgtcaagaaa atttccttga gcgcttccag agagagagaa gggtcttttg
     3301 agacgccttt gaaatatgaa acagagggta tcgaacccgt cgaggctata ggcaagacat
     3361 ctgatattta cagtgaagat gaggctatag gggaggcagg aaggtgtttg aagtgcagat
     3421 gcagaaaatg tattgaatca tgcagccatt taacacgctt taatatatca ccgaaaagct
     3481 acatgaggca aattaatcaa aatgagagga ttatactggg aacccattat gcaaataaaa
     3541 tgattaattc ctgtacaatg tgcgggctgt gcagggaaga atgcttcaaa ggcattagta
     3601 tgaaggctgt cataaaggaa acaagggaga gcatggtaag aaggggcaaa atgcctgttt
     3661 ctgaccatga ttttgcgctg aaggatatga aattcagcaa cagcaagtac ttttcaatgg
     3721 tgagaaatca gggcgaaaaa gctgagtacc tgttctatcc gggatgccaa ctgcctgctt
     3781 catatccgga gtacataggg aaaatatata aatatctggt ttccaatatg tcgggcggtg
     3841 ccggaattta cctgggatgc tgcggggctc ctgccgattg ggcagggagg caggatttaa
     3901 tgcaggagaa cattgagata attaaaaaaa catgggaaga tacgggcagg cctgttttta
     3961 tccttgcctg ctccagttgc tgcagtgtat ttgaaaaata tcttccggaa attgcttttg
     4021 tttccttatg ggagatattt gcccggaaag gtttgcctca aaacaacaga attccgggta
     4081 aacgcgtttt aagcgtgcac gatgcatgtg ctacaaggca taataaggaa atacacgaca
     4141 gtataaggag aatagctgtt ttatcaggct atgaaataca ggagttaaaa tattcaaagg
     4201 agaaaaccaa atgctgcgga tatgggggac tggtgtactt tgccaacagg gaacagtctg
     4261 cagactttgt aaagcatagg attgaagaaa gcgataatga cctgctggta tattgtgcta
     4321 tgtgcaggga tttgcttgcg gagggcggaa aaagaacctt tcacattctt gatttaatat
     4381 tcgcagagga tatggaaagg gctgcactca gaaaaatgcc cactttgtct gaacggcacg
     4441 ggaacagggc gcaggtaaaa atcggattat taagagagat atggggtgaa gagcctgata
     4501 tggattatga aaaacaaagg gatttcaaat tgataatacc tgaaaatgtg cggcagcaaa
     4561 tggaagacag atacattcta tcggaggata tggcagaggt tattgacagt tcaagaaaaa
     4621 gcggagagag gttctttaat cctcaggaga acagctgcct ggcaaggttg agacttgaca
     4681 acgtcaccta ctgggtaaaa tatgaggaaa aggatgagaa gattctggta aagagtgtat
     4741 acagccacag aatggaaata gtggaggaat aggaatgaat gaggataaga attataatat
     4801 gccgtggctc tgcgataaat gcaagaggga actggtgcta aaaaaggtaa aggtacggta
     4861 tttggagggg aatttcgaag ttgatcttcc aaaatgccct gagtgcaata tggtttttat
     4921 agacgaggaa atggctgtgg gcaagatgct tgaggtggaa aaagagttgg aaggcaaata
     4981 aggtgaaggg agatgccgat tatgaatgat attgcattca aactgctcaa gctgtcatct
     5041 tcaggttttt gctgtacgca gataatgctt aagctggcgc tggatgaaga aggaaaggaa
     5101 aatgaggatt tgctgaaagc tgtaagcgga ttgtgcaggg gtataggggg caggcagaag
     5161 agttgcggag tgctgaccgg agggcttgga atccttggcc tttatgctgg gaagggaaag
     5221 gatacggaat atcctaaaca aaactattcc actatgaccg aacagtacct ggaatggttt
     5281 gaaagtgagt tcggaagtga agaatgcaag gatattattg gtgtttatga ttttttcgat
     5341 gatacaggaa acagttatat gataaagtgc ggggatattt tgttaaagag ctatgagaag
     5401 gtagtggaaa tattgagaga caatgattac gagtacggaa gcagggaatg actatgaagc
     5461 agacacctat tgaacagtgg attatgcaaa aaacaggaat aaaggaaagg gacaaaaagg
     5521 cgttggaggc ttatcagctg gggaaaataa aggaaacgat tgcctatgta aaggaaaaca
     5581 gcattttcta ctgtaagcag ttgaaaaata tccgggaaga atcgattaat tctccggaga
     5641 gttttcaaaa tatccccttt acctatcccc gggatattaa gagaaacccc ccagattttt
     5701 tatgcgtgcc tcaaagaaag gttgcaagaa ttgtgacatt aaagacctcc ggtacaagcg
     5761 gtgaggataa aagactgttt tttaccgaag aggatattga acttaccatt gattttttca
     5821 ggcatggcat gagctgcctt gtcgataaaa gcgataaggt gctggtactt atgcccggca
     5881 gcacttacgg aagcatcgga gatttattga aaaaggcatt ggctttatcg gatatagaat
     5941 gtattgtaca tggagttctt accgacccgg atgaagcagc aaggctaatt gaagaaaagg
     6001 gcataacctg catagtcgga atacctttgc aggtgctgta cttaagcaga gttaaaagta
     6061 atgttttcaa gaatagtata aagaaggtgc tgctgagcac agattatgtt cccgatgtac
     6121 taactaatga gttggaacgc agattcggct gcagagtatt cgatcactac ggaatgacgg
     6181 aaatggggta cggcggaggt gttgaatgcg aggctctaaa cggataccac atgagagaag
     6241 gggatatgta ttttgaaatt attgatccgg ataccggtaa ggctgttgaa tgcggacaat
     6301 atggtgaggt tgtttttaca acccttacaa ggcaagctat gccgctgatt cgatacagaa
     6361 caggggatat tgcttcattc tcttcaaatc catgtgcttg cgggacattt ctcaacacaa
     6421 tgaaaaaggt gcttggaaga atcgaaaaca gggtcagaat aggagaagaa aggttcctgt
     6481 atttgaggga actggatgaa atcattctgc cccttgaaga agtcctggac tatagggctt
     6541 acttggatga caaagacaga ttagtgctgg aagtgactgt aaaagacggt attgatttta
     6601 cagaaataaa ggaagaagta atgcggagta tccggaatct gctgtcaaat caatcgaaat
     6661 gcggattaag tgtggagatt gtcaaagcag cagttaaaga acctgcaagc ataaccgaca
     6721 gcatggtaaa aaggaaaata atcgattatc gcaaggagag atgaagattg aaagacatat
     6781 atgaacttat caatgaacag ctggataaaa aagaaagctt tgtgctggct accattctta
     6841 aaaaactcgg ttccgcaccc agggaggaag gcacgaaaat gttgataagg aaggactttt
     6901 caattgaagg aaccataggc ggcggaattt ccgaggctat gaccataaag ctctcagctg
     6961 aggtatttga agatgggcta tatattattg aaaatttttt gatgtccaac aaagactccg
     7021 catgcttggg catggtatgc gggggagata taagcgtact gctggagtat gtggattgca
     7081 acgacagtca gatggtcgat atatacagga aagcaatcga gttgaagcac tgcggcacgg
     7141 attttgttat gataacaaaa ctatcggaag ataggagaaa aatcaggggt tcggataaat
     7201 ggatctgcac cgaaacggga ttctacggta tggagaatga agaaatatgg gaaacaataa
     7261 agaatatcag agtaaacttt aaagaaacca agatcaggga gatattcatc ggaaaagaaa
     7321 ggtacataat agagccgttt tttaatgttg aaagtgttta tatctttggt gcaggccatg
     7381 tagcgcaaaa gatttcagat ataacaaaaa tgcttggctt ctatactgtt gttatggatg
     7441 acagggagga atttgcaaac agggaaagat ttgaaacagc tgatgaggtt agggtgatac
     7501 aatcttttaa taatttggaa aattatgata taattaatgg aaacagttat atagtaattg
     7561 taacaagggg acatgtctat gataaggatg tgcttgccca aatgctcaga accgatgcca
     7621 ggtatatcgg aatgatcggc agcagaagca aaagggaaca tgtctataac aatcttttga
     7681 gtgagggctt tacttcagag gatttggaaa gggttcactg tccgatagga ttgaatattc
     7741 atgcccagac cccggaggag attgctgtga gcattgcagc ggaattgatt aaggttagaa
     7801 gagagcctaa agataaaacg cattaaagcc ggaaacgaga aactgttaca taaaggagaa
     7861 tacatatgaa taatattgat tataagcatg taatagagct ttctcatgaa cgatgcagaa
     7921 aatttaatat taaagaggat caggtttaca gcagcagaat cattaatgaa acggagctgc
     7981 aaaaaagatt tgcagaaaat agaagcatga ttttaacggc agcgccttat atggagcagt
     8041 taataaattt tgtgaagggc cataatttct tcgctcttct tactgatgga gaagggtgta
     8101 tactgaatgc catcggagac gaaaaaatac tgtcggaagc ttttgattta aaaatgatac
     8161 ccggggcttt tatgaatgag gaaagcatcg gaaccaatgc aatgggtatt gttataaaga
     8221 ccggtgaacc cgtgcagttg tcgggtaaag accattttgt aaaggcatat cacagatgga
     8281 cctgttccgg agcacctatc aaggatcaca acgggaacct tattggcgtt ttggatctta
     8341 caggctatac cgagtgtgta caccctcata ccttgggaat ggtcatcgca gcttccaatg
     8401 caattgaaga aatgatcaag gtcaaggagt tcaataatct gcaggatata aacaacaagc
     8461 atataaaaac gatattcaat actatgcctg ttgctattgt aacctccgat atgtacggca
     8521 agataaaaat ttataacaat aaagccctgg agcttttgga taacaaggat aaccaactga
     8581 aggcaaaata tatgagtgaa attattgagg agtgggatag gatcaagcaa gatatctatc
     8641 aagggagaaa tatatcacag gaagtaaata tcattgtgtt tagaaacagg ctctcttgcc
     8701 gtttgattgc caacccaatc tataattcag ttgatgacag tgtagagatc gtatatgctt
     8761 tggaagaaat taaaaaggaa aaaagagata aaaagaaaag cgaataccag gcttactaca
     8821 cctttgacaa aataatagga aaagacgaga attttttgaa aattgttgag tatgcaaaaa
     8881 agatttcaga cagtaagtcc acaatactaa taatgggcga aagcggtaca gggaaagaag
     8941 tatttgctca agcaattcat aattttagta aaagaatgga cggtccttat ctggctttga
     9001 actgcggcgc catacccaat cagcttattg aatcagagct tttcggatat gaggaaggcg
     9061 cttataccgg ggcaaaaaaa ggaggaaatg tcggcaagtt cgaattggcc tcagggggca
     9121 cgattatgct ggacgagata ggtgaaatgc ctctagatat gcaaaccaag cttctgagag
     9181 tccttcagga aggcattatt acaagaatcg gcagcgcaaa gcccatacct gtggatgtca
     9241 gggtaattgc ctctacaaat aaggacttga agaaggaagt acagcttggg agattcagaa
     9301 aggacttgtt ttacaggttg aacgtactcc ccatattttt accggccttg agccagagaa
     9361 aaggtgatat tttgctgctt acagattatt tcatgaaaaa aatcgctgaa aagctcggca
     9421 aaaaagaagt aaagattcca aaaggatata ttaagtctat gatggaatat gattggcccg
     9481 ggaatgtaag agaactggag aattttatag agttgattat ctattccgaa tctattccct
     9541 atgactattt caatcaggaa aaaagctttg ctgaagagct ttatgacaat tacatccaag
     9601 gttcggatat gaacctttgc aatatggaaa aagcacatct gaagaaggta ctgaaaaaac
     9661 acaacggaaa tattacccgt tccgcagaat ccttaggcat aagcagaaat acactgtaca
     9721 gtaaaatgaa aaaatatagg gtaaaggtat aactctttac tttcatttcc catttcacat
     9781 tacaacccat atttgtatat ccttaaaa
//
LOCUS       DAOQUR010000078         9781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7777_length_9781_cov_10.888752, whole genome shotgun sequence.
ACCESSION   DAOQUR010000078 DAOQUR010000000
VERSION     DAOQUR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7777_length_9781_cov_10.888752"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKI50_11255"
     CDS             complement(<1..1115)
                     /locus_tag="PKI50_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HNT04317.1"
                     /translation="MSNIDLPSKFEEFADARRQGFLKVKEIKDKGGKIAGVFCTFTPS
                     EILDAAGLFSVGLCGMSEETIIDAEADLPKNLCPLIKSSYGFALTDKCPYTYFSDIII
                     GETTCDGKKKMYELLGEIKDTYIMQLPQGVDREYAHKMWESELYRLIKTLEEKFQVKI
                     TEEKLRKAAALRNKQRNLYNELFELGKLDPPPIKGFDIYKVIEGSGFSFDVEGNCMRL
                     RELIDNTKKAYEAGNRPVAAGAKRILVTGCPIGGVLDKVVNTIESNGGAVVCFENCGG
                     VKPVRHLVDENAESIISAIADRYLKIGCSVMSPNIRRMEMLPELLKEFKIDGVIETVL
                     QACHPYSVETRTIKKLVNDAGLPYMSIETDYSKSDLG"
     gene            complement(1133..1900)
                     /locus_tag="PKI50_11260"
     CDS             complement(1133..1900)
                     /locus_tag="PKI50_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT04318.1"
                     /translation="MYFTGIDIGSTSAKVVALNENNDILYKNVMPTGWSSTDTAEKIR
                     KDLSIGGIDGGNSKFIATGYGRISVPYADKTITEITCHGKGAAFLYDVDTCTVIDIGG
                     QDTKIISIAKGQVVDFIMNDKCSAGTGRFLEIMASTLGMDLAELCNMAKTGSGISISS
                     MCTVFAESEVVSLIGSGHSKKDIAYGIIESITNKVISLCGKHAATNNKFYLTGGLCES
                     DYIIESLSEKLGSKVISSPLGRYAGAVGAALSAKSLK"
     gene            2168..2359
                     /gene="saoT"
                     /locus_tag="PKI50_11265"
     CDS             2168..2359
                     /gene="saoT"
                     /locus_tag="PKI50_11265"
                     /inference="COORDINATES: protein motif:HMM:NF040730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HNT04319.1"
                     /translation="MKSLEEQYKEQIQVKIYKVGEDFEYIKKYGPTTKSMLIINESRV
                     VTKLSKEAIRKAFEEALKD"
     gene            2356..3450
                     /gene="saoE"
                     /locus_tag="PKI50_11270"
     CDS             2356..3450
                     /gene="saoE"
                     /locus_tag="PKI50_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HNT04320.1"
                     /translation="MIIEFWEFLVNIIYSAINMLNSASVWMVVSFGIAGIVHEYMSPE
                     KMRKSSVGSTKISGVLWTTLAGTGVPICSCGSTPLGISLYYSGAYLGPTLAFMTSTPV
                     LNPVAIILTWGLLGKEVAIINIITGLTVPVIVGIIGNKFAGKELYLEDLEDGVERKTL
                     IFEEPTHFERIKTGLKWSYTELAVTISKYAVSGLLIAGLIFNIVPQSMIQTYLGNPGF
                     ISLFGITVVAALMYVCAVGHIPFIAALVASGAAPGVAITFLMAGAATNIPELLTIYKT
                     IGKRAMIIFLVTLTVLSNVAGYVTNLLLMPGFKPILNYDATSRTVATANKLIFVQPEW
                     LQYVCTSILAAYALYSLYKLIKGEKAKLLS"
     gene            3466..3975
                     /gene="saoC"
                     /locus_tag="PKI50_11275"
     CDS             3466..3975
                     /gene="saoC"
                     /locus_tag="PKI50_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HNT04321.1"
                     /translation="MNRKFHKSAMSIIIVLTIAFAARQYLNVKEEARKNEPTWMRDKE
                     FAAMASEDNIALQYFREKYPERAVLLACEEDINDDKLVDLLIIYKENVDTRLVAVCNT
                     GDGNWYISPEIPAPIENQTIRFKNIDNEAEMEFIVSGEKKGAVGYAVYRLIEGEIKDL
                     FGEGMEDCC"
     gene            3978..4226
                     /locus_tag="PKI50_11280"
     CDS             3978..4226
                     /locus_tag="PKI50_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04322.1"
                     /translation="MNINKRIYFVIVTVMIAGFIIISLLPKDTGRVDSRNALRIGAGD
                     DISGWLLKQIVEISRETGNSERIANSDFITEAYEFQDC"
     gene            4242..4775
                     /gene="saoB"
                     /locus_tag="PKI50_11285"
                     /pseudo
     CDS             4242..4775
                     /gene="saoB"
                     /locus_tag="PKI50_11285"
                     /inference="COORDINATES: protein motif:HMM:NF040727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
     gene            4791..5663
                     /gene="saoA"
                     /locus_tag="PKI50_11290"
     CDS             4791..5663
                     /gene="saoA"
                     /locus_tag="PKI50_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HNT04323.1"
                     /translation="MSISVNGITKEFKNRRERNSNNIVLKDISFNVIEGEFVTLLGPS
                     GCGKTTTLTIIAGFQGHNGGQILINGKEVSKPGPDRAFVFQNYALFPWMKVGENIMYP
                     MKMRKVPKRERKEKLKELLDIAQLSDYENYYVHELSGGMKQRVALLRALACEPQVLLM
                     DEPLGAVDFQMRKLLQIQLENILQKKNVTVLMVTHDVDEAIYLSDRVIIMSRDHGRIL
                     DDIKVNLPRPRDRKDDIYHNYINQLTEILKSALDGDVKNEEDEELLAFIYKNEKKNDV
                     AATKLAAVKEYNPC"
     gene            5697..6029
                     /gene="saoD"
                     /locus_tag="PKI50_11295"
     CDS             5697..6029
                     /gene="saoD"
                     /locus_tag="PKI50_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HNT04324.1"
                     /translation="MRVAYVFESVYAREILENMIIPQLEKGDHGAEVAGMMFFFDNAY
                     MLHKDSDIAKRLAAISKKSGMLLMACDRCCYQREIDDKLIEPAGIGCFPNLYAALAGA
                     NINQVITL"
     gene            6100..7269
                     /locus_tag="PKI50_11300"
     CDS             6100..7269
                     /locus_tag="PKI50_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HNT04325.1"
                     /translation="MMTLPERFNEFAEARRNGFLKVMELKESGANICGSFCQYAPAEV
                     IYAAGLYKVGLCGKSNDPIATAETHLPANLCPLIKSSYGHALEQSCPYAYFSDIVVGE
                     TTCDGKKKMYEMLGKLKPMQVIHLPNVPEEERSLEAWSMEIKHFKEGLEERFDVEITD
                     EKLREEIKWCNKERIQAARIYELGKYDPPAITGLDMRNIMDGEQYMFDRYEKYEKLKQ
                     ILDECVEKWENGNGPYKPDEKPPRILISGAGLGGVVDKTIAVIEELGGAIVCYEGCSG
                     IVSRRRLVEESEDKDPITCIAEKYLEVPCAVMSPNHKRMEQVAATIEEWKIEGLINIT
                     LHSCLPFGVESCNIEKVCKKCDTPYLHIETDFSKGDEGQIRTRIEAFLELIMQRR"
     gene            7331..8107
                     /gene="saoP"
                     /locus_tag="PKI50_11305"
     CDS             7331..8107
                     /gene="saoP"
                     /locus_tag="PKI50_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591054.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:7934..7936,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HNT04326.1"
                     /translation="MTDNRNFRNMVGKGYYFSFFIALLILATIWYFAAHRINKVFIFP
                     YLESVIGELFYACTDLHVLRSLGITLRRVLTGVYYATIIGFPLGMLMGYSPFVMRTIA
                     PFINSFRQIPTTSWVPLAIVWFGLGDGPTIFVIMFHAIFTIILNTVAGVQSISKDFYN
                     AARSMGAKTLDLVKDVVLPGSLSGLITGIRISLGAAWMSVVUAEFIATNSGFGYIIKQ
                     AQLDMITEKVMAYMILAGVLGFAQDRILLVIETFLIRWKR"
     gene            8195..9454
                     /gene="saoX"
                     /locus_tag="PKI50_11310"
     CDS             8195..9454
                     /gene="saoX"
                     /locus_tag="PKI50_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263598084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HNT04327.1"
                     /translation="MNKRILACALAIMMLTVVLTGCQDSSLQKNVVKVDYDVNEEVAP
                     YLAKLPAIPDADKEFVIQMGYNDCDHMVAAIIGENAGIYKALGLNVVVTKSGQTAAAM
                     ASGDMVAGYSGFSSMIKNANKGAPVFMPAGNHLGGSKYFVIKDSVESAEEIIGSRITV
                     GESDMYSPYWLSYCEQVGIPIDYTKYQGFDMGADDAIVAMKAGQLDGFMCCDPFGSVA
                     EMEGIGKIIGTEWGAHVSDDLETGWGLHCGYFINTDFAEEHPELTTRLVLAHCLAIQY
                     MYLHPYNAGMMFADDFGVDPAVGLRTMYLKLCAEGRTLSWDINETNIKNYTDYWHSYG
                     IPEDKIPEVKDLPSFFNLKYLEACGIESFKSFMEDNKINEKFPIRDSYLAWLEKAEEI
                     DGIDHSSTVGKTVEKWNNNEIVSKLLK"
     gene            9475..9723
                     /locus_tag="PKI50_11315"
     CDS             9475..9723
                     /locus_tag="PKI50_11315"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNT04328.1"
                     /translation="MNDSYCVIAFGNTHMAIKVQKTLNEKGFSINMMPTLRAITAGCG
                     LSIRFSSEDLTNIQKAVSEINIEPSLHQFYTVENNMGE"
BASE COUNT         2963 a         1772 c         2181 g         2865 t
ORIGIN      
        1 tgcccaaggt ctgattttga atagtctgtc tcaatggaca tgtatggcag cccggcatca
       61 ttaacaagct tcttgattgt tcttgtttct acactataag ggtgacaagc ctgcaaaacg
      121 gtttcaatta cgccgtcaat tttgaattcc ttaagtaatt caggaagcat ttccattcgt
      181 cttatattag gtgacatgac agagcagcct atttttaaat agcggtcagc tattgcactg
      241 ataatgcttt ctgcgttttc atccactaaa tgccttacag gtttgacacc gccgcagttt
      301 tcaaagcaaa cgactgctcc gccattgctt tcaatagtat ttacaacctt gtcaaggaca
      361 ccccctatgg gacaacccgt caccaatatt ctttttgctc ccgctgcaac cggtctgtta
      421 ccggcttcat aagctttttt tgtgttgtct atcagttcac gaagcctcat gcaatttccc
      481 tccacatcaa agctgaagcc cgagccttca atcaccttgt atatatcaaa gcctttgatt
      541 ggcggagggt ctaatttgcc taattcaaaa agctcgttgt atagattcct ttgtttgttt
      601 ctcagagccg cagcctttct aagcttttct tcagtaattt tgacctggaa tttttcctcc
      661 aaagtcttta tcaaacgata caattcactt tcccacattt tatgagcata ctctctgtca
      721 accccttgag gtagttgcat aatatatgta tccttgattt cacctaaaag ctcatacatt
      781 ttctttttcc cgtcgcaggt tgtctcacca ataataatat cggaaaaata agtataaggg
      841 catttgtccg ttaatgcaaa accataactg gatttgatca ggggacacag atttttcgga
      901 aggtctgctt cggcatcaat tatagtttct tcgctcatgc cgcataatcc cacagagaaa
      961 agacccgctg catccaatat ttcagatggt gtaaaagtgc aaaaaacgcc ggcaatctta
     1021 ccccctttat cttttatttc ttttactttt aaaaagcctt gccttctggc atcagcaaat
     1081 tcttcgaatt tacttggcaa atctatgttt gacataacat accctcctaa tattatttta
     1141 gagattttgc cgatagtgca gctccaactg caccggcgta tcgacctaag ggacttgaaa
     1201 ttactttgga ccctaacttt tcagataggg attctataat ataatcactt tcacaaagac
     1261 caccggtcag ataaaatttg ttattcgtgg cagcatgctt gccgcacagt gatattacct
     1321 tgtttgtgat ggattctatt attccgtatg caatatcctt ctttgagtga ccactgccaa
     1381 tcaggctcac tacttcagac tcggcaaaaa ccgtacacat agagctgata cttatacctg
     1441 agcccgtttt ggccatattg cacaactcag ccaaatccat gcccaatgtg cttgccataa
     1501 tttccaaaaa cctgcctgta ccggcagaac acttgtcatt cataataaaa tcgacaacct
     1561 gacctttggc aatagatata atttttgtat cctgaccgcc aatatctatt accgtgcaag
     1621 tatccacgtc atataaaaat gctgctcctt taccgtgaca ggttatttcc gttatggttt
     1681 tatccgcata gggaacagag attctgccgt agccggttgc tatgaatttg ctgtttcccc
     1741 cgtcaatacc gcctattgat agatcctttc tgattttctc ggcagtatcc gtgctgctcc
     1801 acccggttgg cattacattt ttgtaaagta tatcgttgtt ttcatttagg gcaacaacct
     1861 ttgcagaggt tgaaccgata tcaatcccgg taaaatacaa ttttatctcc tgcctttctc
     1921 ctactaaatt atacatgaaa tatttaatgc acattattgc tattttttat aatggaatat
     1981 ctgtctatca tatttggtaa tatttgtaga aattggtcac ttttattgat atatttgagt
     2041 gtgtaggctt tcttggcggc gaagccgctt ttgttcttta tcaacttaaa gggggggaag
     2101 aacgatgaat aagcctttta tcgagtatat cagtgcttgt gcatgatgcg aagtatttgg
     2161 agaattcgtg aaatctctcg aagaacaata taaagaacaa atccaagtga aaatctataa
     2221 agtcggagag gactttgaat acataaaaaa atacggacct actaccaaga gcatgctcat
     2281 aatcaatgaa tcgagggtag tgacaaaatt aagcaaagaa gccatacgaa aagcatttga
     2341 ggaggcatta aaggattgat aatagaattt tgggagttct tagtcaatat tatttattct
     2401 gctatcaata tgttgaacag tgcatctgta tggatggttg tgagctttgg tattgcagga
     2461 atagttcatg agtatatgag tccggaaaag atgcgcaaaa gcagtgtcgg cagtacgaaa
     2521 atttccggtg ttctctggac aacgcttgca ggcacgggcg taccaatttg cagctgcggc
     2581 agcacccctt tgggaatcag cttatactat tcaggtgctt atttgggtcc cactttggca
     2641 tttatgacat ccactccggt gcttaatcct gttgcaatta ttctgacctg gggattattg
     2701 gggaaagaag ttgcaattat taatattatt acagggctta ccgtgccggt aattgtcgga
     2761 atcatcggca ataaatttgc aggtaaagag ctttatttgg aagacttgga ggatggggtt
     2821 gaaagaaaaa cacttatttt tgaagaacct acacattttg agaggatcaa aaccggttta
     2881 aagtggtctt atactgaatt agcagtgacc atctccaaat atgccgtatc cggtcttttg
     2941 attgccggtt tgatttttaa tatcgtccct cagtctatga tacagacgta tttgggtaac
     3001 cccggtttta tatctctttt tggaatcacc gttgtcgctg cattgatgta tgtgtgcgcc
     3061 gtaggtcata ttccctttat tgcagccctt gtagccagcg gcgcggcacc cggagtagcc
     3121 attacctttc ttatggcagg cgcggcaaca aatattccgg agcttctgac tatatataaa
     3181 actatcggca aaagagcaat gattatcttt cttgtaacac ttacggtttt atccaatgtt
     3241 gcaggatatg ttacaaacct tttattaatg ccgggtttca aaccaatctt aaattacgac
     3301 gccacctccc gaacggtggc cacagccaat aagctgatct ttgttcagcc tgaatggctg
     3361 caatatgtgt gtacatcaat attggcagct tatgcattat actccttgta taagctgata
     3421 aaaggagaaa aggcaaaact attatcatag aaagatgttg aaaatatgaa taggaaattc
     3481 cataaaagtg ctatgtcaat catcattgtt ttaactattg catttgctgc aaggcaatac
     3541 ttgaatgtta aggaagaagc aaggaaaaat gagccgacat ggatgaggga caaagaattt
     3601 gctgcaatgg catcagagga taacatagcc ttgcaatact ttagagaaaa gtaccctgaa
     3661 agggcagtat tattggcttg tgaggaagac attaacgatg ataagcttgt ggatttgctg
     3721 attatataca aagaaaacgt agatacaagg cttgttgcag tttgtaatac aggtgatggg
     3781 aattggtata tatccccgga aatacctgca cctatcgaga atcagactat acggttcaag
     3841 aatattgaca atgaagcaga gatggaattt atagtctccg gtgaaaaaaa aggagcggtc
     3901 ggatatgcgg tttaccgctt gatagaaggg gaaatcaagg atctattcgg agagggcatg
     3961 gaggactgct gctagatatg aatattaata agaggatata ttttgttata gttacagtta
     4021 tgattgccgg ttttattata atctccttgc ttcccaagga taccggcagg gtggatagca
     4081 gaaatgcttt gcgtattgga gccggagatg atattagcgg ttggctgctt aagcaaattg
     4141 tagaaatcag cagagaaaca ggtaatagcg aaagaattgc caatagtgat tttattacgg
     4201 aagcttatga attccaagat tgttgaagta atacctccca atgggccttg agctcagatg
     4261 aaattgatat gggtttttac tgcaatcata aagcaatgca tatggtcaaa ataagtgatc
     4321 gtttggtaat ttacggtccg gtcattatga attcggaagt tatggtctac aaaggtaata
     4381 tcgaggagaa acaagtgctc ggaatagggc aggaacgcaa acatatactg gagttgattc
     4441 aggatagcta ctccaatata aaggaaagca aagaaatgat tcctgcggct ttaccatata
     4501 cattggagaa tgatcagata gatatagcgg ctattgatgt aaccaaagcg gtattattgc
     4561 caagatataa ctttgccgct atttcgaagg atgattatat ttcttactgc ctcgtagtac
     4621 gaaaggattt aatcggcaca gagatctttg atgattttct tgataattat aacaaagcag
     4681 cagaagaact caataagaaa gaaacattga tttcagcctt tggaatgaca agagagtttt
     4741 gggatatggt taatataaag tttttacaac tataatagga agtgtatata atgtcaatat
     4801 ctgttaatgg aattacaaaa gagtttaaaa atcgaagaga aagaaacagc aataatattg
     4861 tactaaagga tatatccttt aatgtgatcg agggagaatt tgttacactg cttggtccct
     4921 ccggttgcgg taagacaaca accttgacca ttatagcagg atttcagggg cataacggag
     4981 ggcagatatt aatcaatggg aaggaagtta gcaaaccggg acctgataga gcttttgtat
     5041 tccaaaacta tgctcttttt ccatggatga aagtcggaga aaatattatg taccccatga
     5101 aaatgagaaa agtgccaaaa agggaaagga aagaaaagct taaagagtta ttggatattg
     5161 cacagctcag cgattatgaa aattactatg ttcatgaatt gtcaggaggc atgaagcaaa
     5221 gagttgcact gcttcgtgca ttggcttgtg agcctcaggt gttacttatg gatgaacctc
     5281 ttggggcagt agacttccaa atgagaaagc ttctgcaaat acaattggag aatattttgc
     5341 agaaaaaaaa tgtaacggta ttgatggtaa ctcatgatgt tgatgaggcg atttatttaa
     5401 gtgacagagt aatcattatg tccagggatc acggcaggat tttggacgat attaaagtta
     5461 atctgccgcg accgagagat aggaaagacg atatttatca taattatata aatcaactga
     5521 ccgaaatatt gaaatcagca ttagatggtg atgttaaaaa tgaagaggat gaagagctat
     5581 tagcgtttat atataagaat gagaaaaaga atgatgttgc agcaaccaag cttgctgcag
     5641 taaaagagta taacccttgc taaagggtat atataaaaat aatgggaggc aggaaaatga
     5701 gagttgcata tgtatttgaa tcagtttatg caagagagat tcttgaaaac atgattattc
     5761 cacagttgga gaaaggggac catggggccg aagtagcggg tatgatgttc tttttcgaca
     5821 acgcctatat gctgcataag gatagcgata ttgctaaaag actggctgca atcagcaaaa
     5881 agtcgggaat gcttctgatg gcgtgcgata ggtgctgtta tcaaagagaa atcgatgaca
     5941 agcttattga accggcggga ataggctgct tccccaattt gtatgctgca ttggccggag
     6001 caaacataaa tcaggttatt acactctagt atccgtcagg agaggcagag cttatgcctg
     6061 cctcttttta aaattagccg aattaaggag taagatatta tgatgacatt gcccgagcgt
     6121 tttaacgaat ttgccgaagc ccgccgtaac ggttttttga aggtaatgga attgaaggaa
     6181 agcggtgcca acatttgtgg cagcttttgc cagtatgctc cggcagaggt tatttacgct
     6241 gcaggactat acaaggttgg tctttgcggc aagagcaatg accctatagc aactgcggaa
     6301 acacatttgc ctgctaattt gtgtcccctt ataaagtcaa gttatgggca tgcattggaa
     6361 caaagctgcc cctatgccta tttttctgac attgttgttg gtgagaccac ctgtgacggg
     6421 aaaaagaaaa tgtatgaaat gctgggaaag ctcaaaccga tgcaagtaat tcatttaccc
     6481 aacgttccgg aagaagagcg ctcattagaa gcctggtcaa tggaaattaa gcattttaag
     6541 gaaggtttgg aagaacgctt tgatgttgaa ataaccgatg aaaagcttcg tgaagaaata
     6601 aaatggtgca ataaagagcg tattcaagct gcgcgtatct acgagcttgg caaatatgac
     6661 ccacctgcta ttaccggctt ggatatgcgc aatattatgg acggcgagca gtatatgttc
     6721 gatagatatg agaaatatga aaagcttaaa cagattttgg atgaatgtgt tgaaaaatgg
     6781 gaaaatggaa atggtcccta caaacccgat gaaaaacctc cccgtatttt gatatccggt
     6841 gccggtctgg ggggcgttgt ggataagact atcgctgtta tcgaagagct gggaggagca
     6901 atcgtttgct atgaaggctg cagcggtatc gtttcccgtc gacgcctggt tgaggaatcc
     6961 gaagacaaag atcctataac ctgcattgcg gaaaaatact tagaggtacc atgtgcggta
     7021 atgtccccga atcataagcg catggagcag gtcgccgcaa caatagagga atggaagatc
     7081 gaaggcctca taaatattac gctgcactct tgccttccct ttggggttga gtcatgtaac
     7141 atagaaaagg tctgcaagaa gtgtgatacc ccatatctgc acatagaaac cgatttttca
     7201 aaaggtgatg agggtcaaat acgtacccgt atcgaggcat ttttagaatt gataatgcaa
     7261 aggaggtaat gtagcagtaa tggctaacga agcaaatagg gatatggatc ggatgctgca
     7321 agcaaccaag atgacggaca atcgtaattt tcgaaatatg gttggcaaag gttactactt
     7381 ttcctttttt attgcgttat tgattttggc tactatttgg tactttgcag cacataggat
     7441 caataaagtc tttatattcc catatctgga aagtgtaata ggcgaattat tttatgcatg
     7501 cacagacctg cacgttttgc ggagcctcgg aataacattg cgaagagtct tgacaggggt
     7561 ttattatgca acaatcattg gttttccctt aggtatgttg atgggctatt caccctttgt
     7621 tatgcgaacc attgcacctt ttataaattc attccgccag attcctacaa cttcttgggt
     7681 tccactggca atagtatggt ttggattagg tgacggacca acgatttttg tcattatgtt
     7741 ccatgcaatt tttactatta tattaaatac agtagcaggg gtgcaaagta taagcaaaga
     7801 tttttacaat gccgcccgca gtatgggcgc caaaacctta gacttggtta aagatgtggt
     7861 gcttcccggt tccctttcgg ggctgattac cggtatacgt atttctctgg gtgcggcatg
     7921 gatgtccgta gtctgagcgg agttcattgc aacaaattcc ggctttggat atataatcaa
     7981 acaggcacaa ttagatatga taaccgaaaa ggttatggct tacatgatac tggccggggt
     8041 tctgggtttc gcacaagaca ggattttact cgtaattgag acattcctga taaggtggaa
     8101 acggtaatac ggaagttttc catattatgt tgcagtgacg atttcccgtg aggaaataga
     8161 tgagatacaa aacatataga aagggagaat ttatatgaat aaaagaatac ttgcatgcgc
     8221 gcttgctatt atgatgctga cagtagtatt gaccggatgt caggatagca gcttacagaa
     8281 aaacgtagta aaagtagatt atgatgttaa tgaagaggtt gctccatacc tcgcaaagct
     8341 tccggcaatt cccgatgcag ataaagagtt tgttatacaa atggggtata acgactgtga
     8401 tcatatggtc gctgccatta tcggtgaaaa cgccggtatt tacaaagctc tcggtctgaa
     8461 cgtcgttgtt actaagagcg ggcagactgc tgccgctatg gcttccggtg atatggtggc
     8521 cgggtactcg ggcttcagtt caatgataaa aaatgcaaat aagggcgctc cggtatttat
     8581 gcctgcaggt aatcatttag gcggctccaa atattttgtt attaaggata gtgtcgaatc
     8641 tgcggaagaa ataatcgggt caagaataac cgtaggtgaa agtgatatgt attctcctta
     8701 ctggctaagc tactgcgaac aggttggtat accaattgat tatactaaat atcaagggtt
     8761 tgatatgggc gctgatgatg ctatagttgc aatgaaagcc ggtcagttag acggctttat
     8821 gtgctgcgat ccctttgggt cagttgctga gatggaaggt ataggcaaaa taatcggtac
     8881 tgagtggggc gcccacgttt cagatgattt ggagaccggt tggggcttac actgcggata
     8941 ctttatcaac acggattttg cagaagaaca ccccgagtta actacaagat tggtgttggc
     9001 acattgtctt gccattcagt atatgtattt gcacccatat aacgcaggaa tgatgtttgc
     9061 cgatgacttt ggcgtagatc cggcagtagg tcttcgtaca atgtatttga aactctgtgc
     9121 tgaagggcgt actttatcct gggatatcaa tgagacaaac ataaaaaact acacggatta
     9181 ctggcatagc tatggaatac cggaagataa aatacccgaa gtaaaggatt tgccgtcctt
     9241 cttcaatcta aaatatctcg aagcctgtgg tatagaaagt tttaagagtt ttatggaaga
     9301 caataagatt aatgaaaagt ttccaatacg ggactcttac ttggcatggc ttgaaaaggc
     9361 agaagaaatt gacggtatcg atcactccag cactgtaggt aagaccgtag aaaagtggaa
     9421 caataatgaa attgtatcga agctgctaaa atagtaataa ggagtgtcat cactttgaat
     9481 gattcatatt gtgtaattgc ctttggcaac actcatatgg ctattaaggt tcaaaaaaca
     9541 cttaacgaaa aaggtttcag tattaatatg atgccgactt tgagagcgat caccgcaggc
     9601 tgtggtcttt ctatacggtt ttcctcagag gatctcacaa atatacaaaa ggctgtttct
     9661 gaaattaata ttgaaccatc attacatcaa ttttatacgg ttgagaataa tatgggagaa
     9721 tagaaagcag cagggacggt tctttttgct tttgaaagca aaaagaaccg tccctgctgc
     9781 t
//
LOCUS       DAOQUR010000079         9609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_7939_length_9609_cov_8.751099, whole genome shotgun sequence.
ACCESSION   DAOQUR010000079 DAOQUR010000000
VERSION     DAOQUR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7939_length_9609_cov_8.751099"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PKI50_11320"
     CDS             complement(<1..985)
                     /locus_tag="PKI50_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT04329.1"
                     /translation="MKKYKIFRTTVISFALLIVFTCVAYADNASKFTDIDNSWAKQHI
                     INVFNKGLMGGATETEFKPGDYVKNYDALVSIARMINREKDINLEQLVEEYKVDVLDK
                     FKVPSYAREAVALCLEKGIVKDSDVSAYSANPYVKKQEVCNYLGRAFGVVVDSSAAVV
                     LPFKDSMFIASQYKAYVSYLMTIGVISETSDNFNPNEQLRRETYAKMLDIASTAYEKK
                     ERGEDITADNGSSEIIWTDYAEPISTDTGTDTDTDTNTEPVAVIEDDEPADVTAHVDQ
                     VIPEYGNLAVFVGTERRVYKLAEDVECTIDGVSEGFWKLEKSDMVKLYTEGS"
     gene            1436..4582
                     /locus_tag="PKI50_11325"
     CDS             1436..4582
                     /locus_tag="PKI50_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04330.1"
                     /translation="MNKKILALLLAFAMMFSSITVAFADESASIGAEAKALETMGVLQ
                     GDTGTVTPEYLAKETTRMQAAIMYLRLKGLEDEAKAFTGTANFADAGTMVWGEGKAMM
                     AYLKANPQLGWIGTDTGKFNPFETITPQQFYKVMLEALGYKQTTAEAEGDFEWSETMA
                     FAAEKGLVKVAEVESFTNNDLAVATMEALKVNYKGMTKTLAVKLVEEGMISKAAAVEA
                     GLYAEEIAVAVKSAKALGNSVVEVTFEDDVDAAAANADNYSIEGLAVKSAVVSAEKKA
                     TLETAAMTSGKVYKLTVGEKTVQFTGIAKVSGGPEIDSVKSEDVEEVVIDFNKNVDYA
                     TATDVNNYSINGVEVVKAEVDGDEVTLTTEGLQNKTKYTVKATNIKSVDGVTKRSNTE
                     TFTVRFDTNAPKIDGEIEVQTNDRIVVKFNEKVTRESAENLDNYTIKVDETDGAELDI
                     LSITWDSDSDDQNSVEIVTEPMEKREDYKLSVNNIADQRKVPNVMTRPATKVYEAIAE
                     DEKAPKWETLTVLSPTTILVEFSDGSKIDEESATDLSNYELEDLDIEAITTIKNEWKT
                     FRAILTVEEMETGKTYDLTIVDILDEFSNAMEETTKPAKAIASSLAASKLDTAKATGK
                     NAVELKFTKEVDEKTAENIANYTITEEVGAPTKATLQADGVTVKLEVNDIINGYDKYD
                     IIVDGVEDLAGNELYYKMDLDTTTEQWDFTAPKLEDADIISSKVVALTFDERVAFEVD
                     EDGDSVTKLVLAKGNETAETASIILNAKGYAEENTVVEFSSNDSLVNGENYTVVGIVY
                     GAGNTDGGVKDLKGNRVLKTSITFGDFTFEGIDDALDLTEVDSYEQVNGKTFEVTMSK
                     NVKFKTGVTVVGGVADIGDFEASIDKNVITLVIEAAKIIEDTDYDINFADFFTDVHGN
                     AVENADDDDSTILTGEYTDDEAPEIESVVAINRETIEITFSEDIPVMDGDAKTAFVNG
                     LDLKNYDLDSNISLSMEADNLSDNDGDNVIRVKVGKPMEARYEYELTVAAGAFKDFVG
                     IANEDEDTFYFDGTNLAK"
     gene            complement(4635..6173)
                     /locus_tag="PKI50_11330"
     CDS             complement(4635..6173)
                     /locus_tag="PKI50_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04331.1"
                     /translation="MFKLNFEKTAVFVLILIICISAADIFRQFFSMSQNSREPYKVDS
                     NITKNIGFPASLLKADQLFKQELYEDAQEEYFKLTNMPSLSPQQKAVVHFKLGVCNYR
                     LKQYDMARNSFLKSAEFNSNDPVAYNNAAVCSFYLNEMEEAEKLQSMAVESLPVIEYY
                     YNLARIYEAWGRYGDSVKYFAAVARAEENITMEDRIDSVRIKNKVIKLTKDSGSIGDF
                     VNELMIALRLKDSREVFIIEDVDMEIKGKSFKWDIVRKNGTNRLSCSYDREKYDPYNL
                     IDSLEWTVRSGGKVIYTAKKDSFSILIDKDKDYIVYLDINYDSNRIATSYFNVQRGSS
                     IYSNGGHETVVSSVAKTKYYEYAVYEQVFEKDFVMSERGYKDRFNCVWGKDDIITRKS
                     DKDFIDAQNALYIKNIANRRAGIWADLSTLIKDKKLNGKTIRVLFYAKKDSEEATLHI
                     GIKTKTGGSYKNTTKRYQLGDKWQRFDFKLSIPEKADGLTMSFKTDVWKEVKIDGFII
                     TVVD"
     gene            6422..7732
                     /locus_tag="PKI50_11335"
     CDS             6422..7732
                     /locus_tag="PKI50_11335"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNT04332.1"
                     /translation="MKDRKRLIVIGSVILIAVIIAVVRIITSGQDRVKEVDIGKAEIK
                     NLSQVITVTGNIEAGNKEEITLPMQHKVIEIFAGEGQKVNSGDPILKIDTTDYEYQLK
                     KYELGLSLALSNLERLQDSGRNSDKKVLENAVKQAELNVKSAEANCNEAKTRYERIKA
                     LYENGAVSKYEYETALKAMNDLNNQHELSVIQLSNALNSLNDFDINRNDQIKDQKNQV
                     ESSKADIENIKDKIEKSTIKSSINGTIVQLDAKPNQYPTAENSTIVIYDLSQYKVTVL
                     ISQYDAVSISPGQKAAIRVKGIDTEYKGTVSAVGDAAVITQEGTEKEPKVEIEVTLDN
                     PDDRIKAGYEADIDITLKEGKDSTAVGFEAVLEDKDGKKYVFAVIDNKAVKTFVETGM
                     ETDFEIEILKGIKAGDSYVKNPPATLKDGDPVKQSGGKNSADKS"
     gene            7716..8426
                     /locus_tag="PKI50_11340"
     CDS             7716..8426
                     /locus_tag="PKI50_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04333.1"
                     /translation="MLIRVEYLTKVYDTGAIQVEALRNVSLSIEKKEYVAIMGASGSG
                     KSTLMNILGCLDRLTDGKYILDDVDVSTLNDNELAVIRNKKIGFVFQAFNLLPRLSAL
                     GNVELPMVYAGIAKSERQEKAKTALEKVGLGDRMHHKPNELSGGQKQRVAIARALVNN
                     PAIILADEPTGNLDTKSSEEIMDIFEGLNNEGVTIVMVTHEREIAQHTKRAVVFRDGE
                     VISDKPMENRIIRGGNNV"
     gene            8423..>9609
                     /locus_tag="PKI50_11345"
     CDS             8423..>9609
                     /locus_tag="PKI50_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT04334.1"
                     /translation="MNIAESFISALQSIKANKMRSFLTMLGIIIGISSVITIVSIGQG
                     GKEAIIGEFDDLGTNVINVNVKSLNNEIEERDYLTLKDARLVREKIPEVTTVVPASYG
                     MGYIKTDKDKKFAEIDGVTQDITKLMKVEIMAGRFLSEGDVEAARNVIVIDNITANKL
                     FKNPENAIGQKVKVTIGDNNSNLTVIGVAESTAGDMAEMFGDNFPGVGYVPITTGDRI
                     IPDARIVMFSVMLENMDNSKEVASKIVHLLEQTHRNEGKYAAEEGFKELDMLNNVINI
                     FTMVIGAIAGISLVVGGIGVMNIMLVSVTERTREIGIRKALGAKNKDIMLQFLTESII
                     LCLIGGIIGMTLGITLGLVVGKIINIPLGISPSVILLAFAFSSAVGIFFGLYPANKAA
                     KLDP"
BASE COUNT         3231 a         1918 c         2023 g         2437 t
ORIGIN      
        1 tgctgccttc agtgtaaagc ttgaccatat cgcttttctc aagcttccag aacccttcgg
       61 aaacaccgtc tatcgtgcac tctacatctt cagccagctt gtatactctt ctctcggtgc
      121 cgacaaatac cgcaagattg ccatactcag gaatcacctg atccacatgg gctgtcacat
      181 ccgccggttc atcatcttca attacagcca caggttccgt attcgtatct gtatccgtgt
      241 ctgtaccggt atcggtactg attggctcag cataatcggt ccatattatt tcagagcttc
      301 cgttgtctgc ggtaatatcc tccccgcgct ctttcttttc gtatgcagta ctggcaatgt
      361 ccagcatttt tgcatatgtt tctcttctta actgctcatt tggattaaag ttatcgcttg
      421 tttcgctgat tacgcctata gtcatgagat aacttacata agccttatat tgagaagcaa
      481 taaacatgga atccttaaaa ggaagcacta ccgccgccga gctgtctaca acgacaccga
      541 aagcccggcc gaggtaattg cagacttcct gcttctttac ataaggattc gccgaatagg
      601 cgcttacgtc ggaatcctta actattccct tctccaggca cagagccaca gcctctcttg
      661 catatgatgg aactttgaat ttgtccagta cgtcaacttt gtattcttcc accagttgtt
      721 ccagatttat atccttttcc cgattaatca ttcgtgctat acttactaat gcatcatagt
      781 tcttgacata atcaccgggc ttgaactcgg tttcggtcgc accgcccatc aagcctttgt
      841 tgaaaacatt tataatatgc tgcttcgccc aactgttatc tatatcggta aacttcgagg
      901 cattatccgc atatgcaaca caggtaaaaa caatcagcag tgcgaaggaa atgactgttg
      961 ttctgaaaat tttatatttt ttcatctgta tcctcctatt atcttcattc ttacaggaat
     1021 atattctgtg gaatttgcac aaatcctgcc tggtaaggaa attgtaacaa aaatatattg
     1081 tacaagcctg gcagaaagag aacctgatta tgttacaaaa ttgtaatgaa acataacaca
     1141 tagcctctgc gtcatattag caggacataa cttcaacctg atggttgaca tagtttatat
     1201 acataattct tatttgtagt actgcgcata ttcctgttac acatttatta cacttgttta
     1261 caatactgtt acatgggaag gaatagcgaa atctgcatag aattatgtta accattagac
     1321 gaattttatc aattttttct tacaacctgg aaagggggtg catggtatag ggtttagaaa
     1381 atgcacaagc agcaaaaaca aaaccaaaaa caattttaag gaggaaataa cacttatgaa
     1441 taagaagata cttgctcttc ttctggcgtt tgcaatgatg ttctcatcta taacagttgc
     1501 attcgctgat gaatcagcgt ctataggcgc tgaagcaaaa gcactcgaaa ctatgggagt
     1561 gcttcagggt gatacaggaa cagtaacacc tgaatacctt gcaaaagaaa caacaagaat
     1621 gcaggcagct ataatgtacc tcagacttaa aggtcttgaa gacgaagcca aagcattcac
     1681 aggaacagct aacttcgcag atgccggcac aatggtatgg ggcgaaggta aagcaatgat
     1741 ggcttacctc aaagctaatc cgcagcttgg ctggattggt acagacacag gaaaattcaa
     1801 cccatttgaa acaattacac ctcagcagtt ctacaaggtt atgcttgaag ctctcggcta
     1861 caagcagaca acagcagaag cagaaggcga cttcgaatgg tcagaaacaa tggcattcgc
     1921 agctgaaaag ggccttgtaa aggttgccga agttgaaagc ttcacaaaca atgacctggc
     1981 agtagctaca atggaagctt taaaggtaaa ctacaaaggc atgacaaaaa ctcttgcagt
     2041 taagcttgtt gaagaaggaa tgatcagcaa ggcagcagct gtagaagccg gactttatgc
     2101 agaagaaatc gcagttgcgg taaaatcagc aaaagctctt ggcaactcag tagttgaagt
     2161 tacattcgaa gatgatgttg atgcagcagc agcaaatgca gacaattact ccatcgaagg
     2221 ccttgcagtt aaatcagccg ttgtttctgc tgaaaagaaa gcaacactcg aaacagcagc
     2281 tatgacatca ggtaaagtct acaaattgac agttggtgaa aagaccgttc agtttacagg
     2341 tattgcaaaa gtatcaggcg gccctgaaat agacagcgtt aagagcgaag acgttgaaga
     2401 agttgttata gacttcaata agaacgttga ctatgcaaca gctacagacg ttaacaatta
     2461 ctcaatcaac ggagtggaag ttgtaaaagc tgaagttgat ggtgatgaag ttacattaac
     2521 tacagaaggg ctccagaaca agacaaaata cacagttaag gcaacaaaca tcaaatcagt
     2581 tgacggtgta acaaagagat ccaacactga aacctttact gtaagatttg atacaaatgc
     2641 tccaaaaatt gacggagaaa tagaagtaca gacaaatgac agaatagtag ttaagtttaa
     2701 tgaaaaggtt acaagagaat cagcagaaaa tcttgacaac tacacaatca aggttgatga
     2761 gacagacggt gcagagcttg atatattatc aatcacatgg gattctgact ctgacgatca
     2821 gaacagcgta gaaatcgtaa cagaaccaat ggaaaaaaga gaagattaca agctttcagt
     2881 aaacaatata gctgaccaga gaaaggttcc caatgtaatg acaagacctg caacaaaggt
     2941 ttatgaagct atagctgaag atgaaaaagc tcctaagtgg gaaaccctta cagtactttc
     3001 acctactaca atattggtag aattcagcga tggcagcaaa atcgatgaag aaagtgcaac
     3061 agacctcagc aactatgaac tggaagacct ggatatcgaa gcaataacaa caataaagaa
     3121 cgaatggaaa acattcagag ccattcttac agtagaagaa atggaaacag gcaagactta
     3181 tgacctgaca atcgttgata tacttgacga attcagcaat gctatggaag aaacaacaaa
     3241 acctgcaaaa gcaatagcaa gcagccttgc cgcatcaaaa cttgacacag caaaagctac
     3301 aggcaaaaat gcagttgaat tgaaatttac aaaggaagta gacgaaaaaa cagctgaaaa
     3361 cattgccaac tacactataa ctgaagaagt cggtgcacct acaaaagcaa cattacaagc
     3421 tgacggcgta acagttaaac tggaagtcaa tgacatcata aacggctatg acaaatatga
     3481 tataatcgtt gacggtgttg aagaccttgc aggaaatgaa ctgtactaca agatggatct
     3541 tgatacaaca accgaacagt gggatttcac agctccaaag cttgaagatg cagacataat
     3601 cagcagcaag gttgtagctc ttacctttga tgaaagagta gcatttgaag tagatgaaga
     3661 tggtgacagt gttacaaagc tggtattggc aaaaggaaac gaaactgccg aaacagcaag
     3721 cattatactg aatgcaaagg gttatgcgga agaaaacaca gttgtagaat tctcatcaaa
     3781 tgattcatta gtaaatggcg agaattacac agtggttgga atcgtatacg gcgctggtaa
     3841 cacagacggc ggtgtaaaag accttaaggg caacagagta ctgaaaactt caatcacatt
     3901 cggtgacttc acatttgaag gaatcgacga cgcactggat ttaacagaag tagattccta
     3961 cgaacaggtt aacggaaaaa cttttgaagt tacaatgtca aagaatgtta agttcaaaac
     4021 cggcgtaaca gttgttggcg gcgtagcaga tataggcgac ttcgaagctt caatcgacaa
     4081 aaatgtaatc acacttgtaa tagaagctgc caagataata gaagatacag attacgatat
     4141 caattttgcc gacttcttta cagacgtgca cggcaacgct gttgaaaacg cagatgatga
     4201 tgattcaaca atcctgacag gtgaatatac cgatgatgaa gcacctgaaa ttgaatcagt
     4261 tgttgcaata aacagagaaa ctatcgaaat aacattcagc gaagatattc ctgtaatgga
     4321 tggagatgca aagacagctt tcgtaaacgg ccttgacctc aaaaactatg atttggacag
     4381 caacatttca ttatccatgg aagctgataa cttaagcgac aatgacggtg acaacgtaat
     4441 aagagttaaa gtaggaaagc caatggaagc aagatacgaa tatgaactga cagtagcagc
     4501 aggagcattc aaggatttcg taggcattgc aaacgaagac gaagacacat tctacttcga
     4561 cggtacaaac ctggcaaaat aatttaacca aaaacaatta aaggatgagc gacctgctca
     4621 tccttctttt ttctctaatc tactacagta attataaacc cgtctatttt tacttccttc
     4681 caaacatctg tcttgaagct cattgtcagg ccgtccgctt tttcaggtat tgacaatttg
     4741 aaatcaaatc gctgccactt gtcaccaagc tgatatcttt tggtggtatt cttatatgaa
     4801 cctcccgttt tggtttttat tccgatatgc agcgttgctt cttcagagtc tttttttgca
     4861 tagaacagaa cccttatagt tttgccattc agcttcttat cctttatcaa ggttgacaag
     4921 tctgcccata ttcccgccct tctgttcgca atgttcttta tatacaacgc attctgcgca
     4981 tctataaaat ccttgtccga ctttctggta attatatcgt ccttgcccca gacacagtta
     5041 aacctgtctt tgtagcctct ttcactcatc acgaagtctt tttcaaaaac ctgttcatat
     5101 acagcgtatt cataatattt tgtttttgca accgacgaca caacagtctc atggcctccg
     5161 ttggaatata tgctgctgcc tctctgcacg ttaaaataac tggttgcgat tctgttagag
     5221 tcataattga tatcaagata tacaatataa tccttgtcct tatcaatgag aattgaaaaa
     5281 ctgtcttttt tcgcggtata tataactttt ccgccgcttc gcacagtcca ttccagtgaa
     5341 tcaatcaaat tatacgggtc gtacttttcc cggtcgtaag agcaggaaag cctgttggtg
     5401 ccgtttttcc ggacaatgtc ccacttgaag cttttaccct taatctccat atcaacatct
     5461 tcaataatga atacttcccg tgaatcctta agcctgaggg caatcatcaa ctcatttaca
     5521 aaatcgccga tactgcccga atccttagta agctttatca ctttattttt tattcgaacc
     5581 gaatctatcc tgtcttccat ggtgatgttc tcttccgctc ttgctaccgc cgcaaagtat
     5641 ttaaccgagt ctccgtatct gccccaggcc tcatagatcc tggcaagatt gtagtaatat
     5701 tcaatgaccg gaagactttc aaccgccatg ctctgaagct tttcggcctc ttccatctca
     5761 ttcaggtaaa aagaacatac cgcggcatta ttatatgcaa ccggatcgtt gctgttgaat
     5821 tcggcagatt ttaaaaagct gtttctggcc atgtcatatt gcttaagcct gtaattgcat
     5881 actcccagct tgaaatgaac cacagccttc tgctgcgggc tcaggctcgg catgtttgtg
     5941 agcttgaagt attcttcctg cgcatcctca taaagctcct gcttgaacag ctggtcagcc
     6001 ttaagaaggc ttgccggaaa gcctatattc ttcgtaatgt tgctgtcgac tttataaggt
     6061 tctctgctgt tttgagacat gctgaaaaat tgtctgaaaa tatcagcggc agaaatgcat
     6121 ataatcaata tcaatacgaa tactgcagtt ttttcaaaat taagcttgaa caagcattca
     6181 ccccctcgcc tcaattatat tgtattataa aaacgcaaaa cccgcaattg attaacataa
     6241 tatagcttgc accctatgac aagataatta tatactgtat atgtcatgaa aaagattttt
     6301 catgacatat gaatcaataa aaatgtttag tgtaaaacat ctgtttgtaa acttttttga
     6361 ttcttttacg tatagaattg tgtagatata aagtgcaatg tatttttaag ggggaaaaac
     6421 gatgaaggac aggaaaaggc tcattgtcat agggtctgtt atactgatag cagttattat
     6481 tgccgttgta agaataataa catccggtca ggacagagtc aaagaagtag atatcggaaa
     6541 ggctgaaata aaaaatttga gccaggttat cacggtaaca ggcaatatag aagcaggaaa
     6601 taaagaagaa ataacgctgc ctatgcagca taaggtaata gaaatttttg ccggagaagg
     6661 ccaaaaggtc aattcgggag atccgatcct gaaaatcgat accacagact atgaatacca
     6721 gctaaaaaaa tatgagcttg gactcagcct tgccctctca aacttggaaa ggcttcagga
     6781 cagcggcaga aacagcgaca aaaaagtttt ggaaaatgca gtaaagcaag cagagttgaa
     6841 tgtcaagagc gcagaagcaa actgcaatga agcgaaaaca agatatgaac gaatcaaggc
     6901 actttatgaa aacggcgcag tttcaaaata tgaatatgaa accgccctta aggcaatgaa
     6961 cgacctgaat aaccagcacg aattgtccgt aatacagctg agcaacgctt taaattccct
     7021 taatgatttt gacataaaca ggaatgatca gataaaagac cagaaaaatc aagtggaaag
     7081 ctcaaaagcg gatatagaaa atataaagga caaaattgaa aaaagtacaa taaagtccag
     7141 cattaacggc accatagtac agcttgacgc aaaacctaac cagtatccca cggcagaaaa
     7201 cagcaccata gtgatatatg acctttcaca atacaaggtg accgtactga taagccaata
     7261 tgacgcagtc agtataagcc ccgggcagaa agcagccata agggtaaagg gaatagacac
     7321 ggaatacaaa ggcacagtat ctgctgtcgg ggatgcggct gtcattaccc aggaaggaac
     7381 ggaaaaggaa ccaaaggtgg aaattgaagt cactcttgac aatcctgacg acaggataaa
     7441 ggcgggctat gaagcagata tagatattac cctcaaagag ggcaaggact caacagcagt
     7501 gggctttgaa gcggtcctgg aggataagga cggcaaaaaa tatgtatttg cggtcattga
     7561 caataaagcg gtcaaaacct tcgtagaaac gggcatggaa acagacttcg agatagagat
     7621 actcaaaggt ataaaagcag gtgattccta tgtaaagaat cctcctgcaa ccctgaagga
     7681 cggggacccg gtaaagcagt cagggggtaa gaacagtgct gataagagtt gagtacctta
     7741 caaaagtgta tgatacaggg gcaatacagg tggaagcctt aaggaacgta tccctttcca
     7801 tagaaaagaa ggaatatgta gcaataatgg gggcttccgg ctccggcaaa tccacactga
     7861 tgaacatatt gggatgcctt gacaggctta cggacggaaa atacatactt gatgatgtag
     7921 acgtatccac ccttaatgac aatgaactgg cagtaataag aaataaaaag ataggctttg
     7981 tgtttcaggc ctttaatctg cttccgaggc tgtccgcctt aggcaatgta gagcttccca
     8041 tggtttatgc gggtatagca aaaagcgaaa ggcaggaaaa ggccaaaacc gcccttgaaa
     8101 aggttggcct gggagacaga atgcaccata agccaaatga actttcaggg ggacagaagc
     8161 aaagagtggc catagcaagg gctcttgtga acaaccctgc tataatactt gcggatgaac
     8221 ctaccggcaa ccttgataca aaatccagcg aggaaataat ggatatattt gagggattaa
     8281 ataatgaagg tgttacaatt gtaatggtaa cccatgaacg ggaaatagca cagcatacca
     8341 agagagcagt ggtattcaga gacggcgagg taatatccga taaaccaatg gaaaacagga
     8401 taatacgagg ggggaataat gtatgaatat agctgaaagc ttcatatcag ccctccaaag
     8461 tataaaagcg aacaaaatgc gctccttcct caccatgctg ggtataataa tcggcatttc
     8521 atcggttata accatagtat caatagggca gggaggcaag gaagccataa taggtgaatt
     8581 tgacgacctg ggcaccaatg taataaatgt aaacgtcaaa tccttgaaca atgagattga
     8641 ggaaagagat tatttaacac ttaaggatgc ccggcttgtg agggaaaaaa tccctgaagt
     8701 caccacagtg gtaccggcct cctatggcat gggatatata aaaaccgata aggacaaaaa
     8761 atttgctgaa atagacggag tcacacagga tatcaccaag cttatgaagg tggaaataat
     8821 ggccggaaga ttcctcagtg aaggcgacgt agaggcagca agaaatgtaa tagtaatcga
     8881 taatattaca gcaaacaagc tttttaagaa ccctgaaaat gccataggtc aaaaagtcaa
     8941 ggttacaatc ggagacaata attcaaatct tactgtcata ggcgttgcgg aaagcacagc
     9001 gggggatatg gcggaaatgt tcggggacaa ctttccgggt gtaggctatg ttcctataac
     9061 taccggagac agaataatac ctgacgcaag aattgtaatg ttttccgtta tgcttgagaa
     9121 tatggacaat tcaaaggaag tagcatccaa aattgtgcat ttgctggagc agacccacag
     9181 aaacgaaggg aaatatgctg cagaggaagg cttcaaggag ctggatatgc tcaataatgt
     9241 aattaatata tttacaatgg tcataggggc tattgcaggc atatcccttg tagtaggcgg
     9301 cataggcgtt atgaatataa tgctggtatc ggtaaccgaa agaaccaggg aaataggcat
     9361 aagaaaagca ctgggagcca aaaataagga tattatgctc cagttcctca cagaatcaat
     9421 tatactgtgc ctcatcgggg gaataatagg aatgaccctg ggaataacct tgggattggt
     9481 tgtcggaaag ataattaaca ttcctctggg gatatcaccc tcggtaatac ttctggcttt
     9541 tgccttctcc tcagcggtag gtatattctt cggactgtat ccggcaaata aagccgcaaa
     9601 gcttgatcc
//
LOCUS       DAOQUR010000080         8678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_9002_length_8678_cov_9.914183, whole genome shotgun sequence.
ACCESSION   DAOQUR010000080 DAOQUR010000000
VERSION     DAOQUR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_8678_cov_9.914183"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>1096
                     /gene="ychF"
                     /locus_tag="PKI50_11350"
     CDS             14..>1096
                     /gene="ychF"
                     /locus_tag="PKI50_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNT04335.1"
                     /translation="MKIGLVGLPLVGKTTFFNLLTNAHAEISNFASGKVASNIGSAKI
                     PDRRIDFLSKLYKPKKTTFAMIDFTDVPGLVSGASTGAGVGNQFLEDIRKVDALVHIV
                     RAFENTDVLHIEGSIDPMRDIETVNLELLFADLGIIDNRIDRIKSGKKVTKENLEELK
                     VLEKCKEGLENGLLIHQINLSEEERDLLKTFSFLSEKPMILVINIDEKSLKSDSYPQK
                     EDIRKYAAERNVPVIEVCAKAEMEINELDEEDRLMFMEELGIKSSGIDILANTTYDYL
                     GLISFLTAGEDEVKAWTIRKGTDAKKAAGKIHSDIERGFIRAEVVKFKDIEALGSMAK
                     VKEKGLYRLEGKEYIVQDGDIINFRFN"
     assembly_gap    1097..1196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1274..2569)
                     /locus_tag="PKI50_11355"
     CDS             complement(1274..2569)
                     /locus_tag="PKI50_11355"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNT04336.1"
                     /translation="MNYNEVVDLIDTTVKFGCKPGLERTEKLLELLGNPHKKLRVIHV
                     AGTNGKGSTAAMLASILSSAGYRTGMYISPHLFRNTERMTIDGIEITEEDFAEYATQV
                     LSMVRLMGEKGLEEPTQFEMYTAMAFLYFERKKVDFAVVEVGLGGRFDATNAAEPLLS
                     VITSIGYDHKDVLGDTIEKIAYEKAGIIKEGSMAVMYPQPYPEASAVIETVCREKGAI
                     LAEADIDSLILKDYGLSGQTVDFKYRDYDIRGMKLPLIGDHQLYNAAVALTAIAELDK
                     MGHRIEEAGIRKGIGSVKWPCRLSIVSIEPLILVDGAHNEDGINSLRNALLKYFGNRK
                     IIFVIGMLRDKDYAYAIRKLMPMAYHVVAAEPFSERALSASAMAEAVKPYCSSVISEP
                     DIKKAIEKAKKLYEKDCMICICGSLYLAGRAYEYLTEKN"
     gene            complement(2586..5243)
                     /locus_tag="PKI50_11360"
     CDS             complement(2586..5243)
                     /locus_tag="PKI50_11360"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNT04337.1"
                     /translation="MDESKNIAKTYDPKQVEERLYEIWMEKGYFTPVIDKNKEPYTIV
                     IPPPNITGQLHMGHAMDNTIQDILIRWRRMQGYCTLWLPGTDHASIATEAKIVETLAK
                     EGKTKYDLGREKYLEMAWDWKHEYGGRILNQLKKLGSSCDWTRERFTLDEGCSDAVLE
                     VFVRLYEKGLIYRGERIINWCPKCKTSISDIETIYDTAKGHFWHVKYPVKDSDEYIEI
                     ATTRPETILGDTAVAVNPNDDRYRHLVGKTLILPLMNREIPVIEDEYVDMEFGTGAVK
                     ITPAHDPNDFEVGTRHNLPMPRILNDDGTINSLGGKYKGLDRYDARKQIVKDLEEQEL
                     LVKTKEHEHNVGHCQRCSTTIEPLLSKQWFVKMKPLAEPAIEVVRGGRVKFVPERFTK
                     IYYNWMENIQDWCISRQLWWGHRIPAYYCEDCGEVIVSKTAPGSCKKCGGKLKQDEDT
                     LDTWFSSALWPFSTLGWPEKTEDLEYFYPTSVLVTGYDIIFFWVARMIFSAMENMQEE
                     PFRHVLIHGIIRDSEGRKMSKSLGNGIDPLDVIDKYGADALRFNLISGNSPGNDMRFF
                     WEKVEAYSNFANKIWNASRFVLMNLDYDRVKNAVPSEYSSSLTAADKWIISRFNTVVK
                     EVTENMEKFELGIAAQKIYDFMWSEFCDWYIELVKPRLYGDDEETKLVAQATLCDILK
                     GTMQLLHPFMPFITEEIYRHLPSEYESIVISRWPEYNGQAYQPEEERRMEKVMEAIRG
                     IRNVRAEMNVVPSRKAKAIVVTADKDVKEAIEEGRLYFERLASASEVVIQDSKEGIPE
                     GAVSVLINGAEIYLPLEDLIDFEKELERLNKEKDNLEKELQRVKGKLSNQGFIGKAPR
                     SVIEEERTKEKKYQEMLNKIYERINSLKK"
     misc_binding    complement(5304..5515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5674..6207)
                     /locus_tag="PKI50_11365"
     CDS             complement(5674..6207)
                     /locus_tag="PKI50_11365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT04338.1"
                     /translation="MNIPNALTIFRLILIPGFVYYYFSSMPNGDRIAIVLFAAAGVTD
                     ILDGFIARRCNLITRLGTVLDPLADKLMLLTVLISATMKNQISFWIIIVVAIKETLLI
                     LGAITLFNDHDIVVPANRFGKLSTIAFYIAIIAVAFDIPYSRVMLDGFVIITILALVV
                     YANCYINIKRRQRIYKT"
     gene            6451..7470
                     /locus_tag="PKI50_11370"
     CDS             6451..7470
                     /locus_tag="PKI50_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT04339.1"
                     /translation="MRKSAKLVSLVLVVLIVLSSAAMLSAGCSRKELTTVRLNEVVRS
                     IFYAPQYVAINKGFFEEQGLEIELTTGQGADKTMTALLSGQCDIGFAGPEASIYVYEQ
                     GKEDYAVIFAQLTQRDGSFLVARNDEPEFKWENTRGKVIIGGRPGGVPEMTLEYVLKK
                     YGIIPGKDVEIITNLQFTATAGAFISGVGDYVALFEPTASMLEKEGAGKIVSSLGEES
                     GLIAYTAYFTSESVIDKNPEMIQKFTNAFYKGQLWVHSHTAEEIAEAIKSFFPDADDD
                     ILVTVVKRYKEIDAWCTDPITTPESLDLLQEVMQTAGELDKKVEYDKIVTQEFARKAI
                     ETVKP"
     gene            complement(7499..8530)
                     /gene="mnmH"
                     /locus_tag="PKI50_11375"
     CDS             complement(7499..8530)
                     /gene="mnmH"
                     /locus_tag="PKI50_11375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNT04340.1"
                     /translation="MYNTIDYEQLEGDYVLVDVRSPKEYSEFTIPGAVNLPIFNDEER
                     ELIGTVYNCGSIDEAKKMGVDFASKKLPLLYEEIKRLREGHDKVILFCERGGYRSSSL
                     CALLNSIGIGAVKLRGGYKGYRAVVNSMLPKLNREVNYIVIHGYTGTGKTKLLKMLES
                     RGYDILDFEMHANHRGSLLGDVGLGKRISQKQFESLVYDSLKKRKSDMIFVEGESSRI
                     GNIVIPHYIMQSMREGKHILAVGSIEMRVRRIVEEYTKEKNSDKDIINSLEKLGRNIS
                     ARRVEEYARDIRLRNYHQVAEDLMIRYYDPMYENEMKEFEYELTVNTDNIQKACLDME
                     KWLKKAKPE"
BASE COUNT         2276 a         2108 c         1632 g         2562 t
ORIGIN      
        1 ggaggtaata tccatgaaaa ttggtctggt tggtctgcct cttgtaggca aaacaacttt
       61 ttttaatctg cttaccaatg ctcatgcgga gatttctaat tttgcctcgg gaaaagtggc
      121 ttcaaatata ggctccgcaa aaattcccga ccgcagaatc gattttcttt caaagctata
      181 taaaccgaaa aagacaactt ttgcaatgat agactttacc gatgtccccg ggctggtcag
      241 cggggcaagc accggtgccg gagtaggcaa tcaatttctt gaggatatca gaaaggtcga
      301 tgccctggta catatagtaa gggcctttga aaacactgac gtgctgcata ttgaaggttc
      361 aatagaccct atgcgtgaca tagaaacagt aaatttggaa ctgcttttcg cagatttggg
      421 cattatagac aatagaattg acaggattaa aagcggcaaa aaggtaacaa aagaaaacct
      481 ggaagagctt aaagtattgg aaaaatgcaa ggaaggcctt gagaacgggc ttctgataca
      541 tcagataaat cttagcgaag aagaaagaga cctgctgaaa accttcagct ttctttctga
      601 aaaaccgatg atactggtaa taaacattga cgaaaaatcc cttaagtcag acagctatcc
      661 ccagaaggaa gatattcgga agtatgcagc cgaaagaaat gttcccgtta ttgaggtatg
      721 tgccaaagca gaaatggaaa ttaacgagct tgatgaagaa gacaggctta tgttcatgga
      781 ggaactgggc ataaaaagct ccggaattga tatattggca aacaccactt acgattatct
      841 gggattgatt tctttcctga cggcaggtga agacgaggta aaagcctgga ctataagaaa
      901 agggaccgat gcaaaaaagg ccgccggcaa aatccactcg gatattgaaa gaggattcat
      961 cagggctgaa gtggttaaat ttaaagatat cgaggcgctg ggctcaatgg caaaggttaa
     1021 ggaaaaaggc ctctacaggc ttgaaggaaa ggaatacata gtacaggacg gagatataat
     1081 caatttcagg tttaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggg
     1201 acgggacatg gggacgtttc ccctgtcccg caagcgggac acgcgaaacg tccccatgtc
     1261 ccgtccccgt gtctcaattt ttttccgtta agtactcata ggcacgtcct gccagatata
     1321 aagagccgca tatacatatc atgcagtcct tttcatacag tttctttgct ttttcaattg
     1381 cttttttaat gtcaggctct gaaattacac tgctgcagta aggcttcacc gcctctgcca
     1441 ttgcagaggc gctcagtgct ctttcactga aaggctccgc ggctaccaca tgataagcca
     1501 tcggcataag ctttcttatg gcataagcat agtctttatc tctcagcatt ccgattacaa
     1561 aaataatctt tcgattgccg aaatacttca ataacgcgtt acggagagaa ttgattccat
     1621 cctcattgtg tgctccgtca acaagtatca aaggctcgat gcttactatg ctcaaacggc
     1681 aaggccattt aacagaccca atcccctttc tgatacccgc ttcttctatc cggtgcccca
     1741 ttttatcaag ctccgctatg gcagtcaatg caacagcggc attatataac tgatgatccc
     1801 ctataagcgg cagcttcata ccgcgaatat cataatcccg gtatttaaaa tcaactgtct
     1861 ggccgctaag gccataatcc ttgagtatca gggagtcaat atccgcctct gccaaaattg
     1921 cacctttctc cctgcacact gtttcaataa cagccgatgc ttcaggatac ggctgcggat
     1981 acattaccgc cattgaacct tcctttatta tacctgcctt ctcataggct atcttttcta
     2041 tagtgtctcc gagtacatcc ttgtggtcat agcctattga ggttatcaca gaaagcagag
     2101 gttctgctgc atttgtagca tcaaaccttc ctcccagccc aacctcaaca actgcaaagt
     2161 caaccttctt acgctcaaaa tacaggaaag ccatagcagt atacatttca aactgagtag
     2221 gctcctcaag ccctttttcg cccataagcc ttaccatgct caatacctgc gtggcatatt
     2281 ctgcaaaatc ctcttccgta atttcaatgc cgtctatagt cattctttca gtattcctga
     2341 acagatgagg cgatatatac atacccgttc tatatcctgc actgcttaga atactggcaa
     2401 gcattgccgc tgttgagcct ttgccgttgg tgcccgctac atgaatcacc ctgagctttt
     2461 tatggggatt tcccagcagc tccagaagct tctcagttct ttcaaggccg ggtttgcatc
     2521 cgaatttcac cgttgtgtct atcaggtcca caacttcatt atagttcatc gctttttact
     2581 ctccgtcatt ttttcagtga gtttattctt tcatatattt tattcagcat ttcctggtat
     2641 ttcttttctt tagtccgctc ctcttctatt acactccggg gggcttttcc tataaagccc
     2701 tgattgctga gctttccttt gaccctctgc agttcctttt ccaggttatc cttctccttg
     2761 ttaagccttt caagctcttt ttcgaaatct atcaggtctt caagaggcaa atatatctca
     2821 gcaccattaa taagtactga tactgccccc tccggaatgc cctccttgga atcctgtata
     2881 acaacctctg aagcagaagc cagcctttca aagtatagcc tgccttcctc aatagcctct
     2941 tttacatctt tatctgctgt tactacaatt gccttggctt ttctggaagg aacaacgttc
     3001 atctcagctc ttacatttct tatgcccctt attgcttcca tgaccttttc catcctccgc
     3061 tcttcttccg gctgatatgc ctgcccatta tactccggcc acctggaaat cacaattgat
     3121 tcatattccg atggcaggtg ccggtatatc tcttccgtaa taaacggcat gaatgggtgc
     3181 agcagctgca ttgtcccttt taatatatcg cacagggtag cttgtgccac cagctttgtt
     3241 tcctcgtcat ccccgtaaag ccttggcttt accaactcaa tataccagtc gcagaattcc
     3301 gaccacatga aatcataaat cttctgtgcc gcaatgccca actcaaattt ttccatgttt
     3361 tcggtaactt cttttactac ggtattgaac ctgcttatta tccacttgtc cgccgcagtc
     3421 aggcttgatg agtattccga cggaactgca ttctttaccc tgtcataatc caggttcata
     3481 agaacgaatc ttgaagcgtt ccatatctta ttggcgaagt tgctgtaggc ctcaaccttt
     3541 tcccagaaaa atctcatatc atttccggga gagttgccgg atatcaggtt gaatctcaat
     3601 gcatccgcac cgtatttatc aattacatca agagggtcta ttccgtttcc gagagatttg
     3661 ctcattttcc tgccttcaga atccctgatt atgccatgaa tcaggacatg cctgaaaggc
     3721 tcttcctgca tattctccat tgctgagaaa atcattctgg caacccagaa gaatatgata
     3781 tcatatccgg taaccagtac gcttgtagga tagaaatact caagatcctc agtcttttcc
     3841 ggccagccaa gggtggaaaa aggccacaag gcagaactga accaggtatc cagggtatcc
     3901 tcatcctgct taagcttccc tccgcatttt ttgcaggagc ccggtgctgt ttttgataca
     3961 attacctctc cgcagtcctc acagtagtaa gcgggtatcc tgtgccccca ccacagctgt
     4021 cttgaaatgc accaatcctg aatgttttcc atccaattat aatagatctt cgtgaacctc
     4081 tccggcacga attttaccct gccgcccctt acaacctcta ttgcaggctc agcaaggggc
     4141 ttcatcttaa cgaaccactg ctttgaaagc aaaggctcta ttgttgtgct gcacctttgg
     4201 cagtgtccca cattgtgctc atgttctttt gttttaacca acagctcttg ttcttccaaa
     4261 tctttaacaa tctgcttcct tgcatcatac cgatccagcc ctttatattt accgcctaag
     4321 ctgtttattg taccgtcatc attcagtatc cttggcatcg gaagattgtg tctcgtgcct
     4381 acttcaaagt cattggggtc atgggccggt gttatcttaa ctgctccggt tccaaactcc
     4441 atatcgacat actcatcttc tattaccggt atttctctgt tcataagcgg cagtatcaat
     4501 gtcttgccga cgagatgcct gtacctgtcg tcatttggat taaccgctac ggcagtatct
     4561 cccagtattg tctccggcct tgtagtggca atttctatat attcgtcact gtcctttacg
     4621 gggtacttca catgccagaa gtgccctttc gcagtatcat aaatagtctc aatatcagat
     4681 atggaagtct tgcacttggg gcaccagttt attattctct cacccctgta tataagcccc
     4741 ttttcatata atctgacaaa aacctccaaa accgcatctg aacagccttc atccagggta
     4801 aacctttcac gggtccagtc acaggagctt ccaagttttt tcagctggtt aagtatcctt
     4861 ccgccgtatt catgcttcca gtcccaagcc atctcaaggt atttttctct tcccaaatca
     4921 tacttcgttt ttccctcttt tgccagagtc tcaactattt tggcctctgt agctatgctt
     4981 gcatgatccg tacccgggag ccagagtgtg cagtatccct gcatccttct ccaccttata
     5041 agtatatcct gtatagtatt gtccattgca tggcccatat ggagctgtcc ggtaatattc
     5101 ggcggaggta tcactatagt atagggctct ttgttcttat cgatcacagg agtaaaatat
     5161 cctttttcca tccagatctc atacaatctc tcttctactt gtttcgggtc ataagttttt
     5221 gcaatatttt ttgattcgtc catgttaaat cctcctcgca gttgaatttg aaaagttctg
     5281 taaaataaaa aacccccgcc catgttaagg gcgaaggtta ttcgcggtac caccttattt
     5341 ccggcaaaca ccggctcttt gaactgtaac gggcagaccc gcctgatgct actctattca
     5401 cagcaggaac tcagaagcta ccttcagcag tgccgtaccc ggaaaacctt tcagcctgtg
     5461 ggtttccctc tctaaaaggc ggttaatgcc tactcctctt cctcattgct tttttgaggc
     5521 tattggcaat tggctatagg gtatgggctg catgccaggc ttcgaagccc tgctgaacct
     5581 attacctatc gcctattgtc tcttgccttc taattattat atatcttatt agagttgtgc
     5641 ggatttgtca atattatttt tttatcaggc gtttcacgtt ttatatatcc tctgccggcg
     5701 ctttatattt atataacaat tcgcatatac caccaatgcc agaatagtaa ttatcacaaa
     5761 cccgtcaagc attaccctgc tgtatggtat atcaaaagca acagcaataa tcgctatata
     5821 aaacgcaatt gtggagagct tcccgaacct gtttgcaggt accactatgt catgatcatt
     5881 gaacagcgtg attgctccca atatcagcag agtctccttt atcgccacga caataattat
     5941 ccagaaggaa atctgattct tcattgttgc gcttataaga acagtaagca gcatcagctt
     6001 atccgccagg ggatcaagca ctgttcccag ccttgtgata agattgcacc tgcgtgcgat
     6061 aaagccgtca agtatatctg tcacgcccgc cgccgcaaac agtactattg ctattctatc
     6121 cccattcggc attgaagaaa agtaatagta gacaaaaccc ggaatcagta taagtctgaa
     6181 aattgtcaat gcatttggaa tattcatagt tacctctccg tactacattt tcatatatgc
     6241 agttgttttt tgaacgaccc gctcttccga gttgatattt ttatgttccc ccataagtgt
     6301 aataaatatt atactgttcc aataccagtt tatgctttgc ctctgcggca gttcatcaaa
     6361 aaatccttgg actgtctcaa aaaaatatat atcacataat atgcaaataa tagcagcagt
     6421 cgtatccaaa ctatgatagg ggggtatttg gtgagaaaat ctgccaaact tgtatcactt
     6481 gtactcgttg tccttattgt gctttcttct gcggcgatgc tttccgcagg ctgcagcagg
     6541 aaagagctta caaccgtcag gcttaatgaa gtggtgcgct caatctttta tgctccgcag
     6601 tatgtagcta tcaataaggg cttctttgaa gaacagggcc tggaaataga actgacaaca
     6661 ggccaagggg cggacaagac aatgaccgca ttgctttccg gccaatgcga catcggcttt
     6721 gcgggacctg aagccagtat ctatgtttac gaacagggaa aggaagatta tgctgtaata
     6781 tttgcacagc ttacccaaag ggacggttcc ttcctggtcg cacgcaacga tgaaccggaa
     6841 ttcaaatggg aaaacacaag aggaaaggta ataatcggcg gaaggcccgg gggcgtgccc
     6901 gaaatgacac ttgaatatgt attgaagaag tatggaatta taccgggtaa agacgtcgag
     6961 ataatcacca acctgcagtt tacagctaca gcaggagctt tcataagcgg ggtcggagac
     7021 tatgtggcat tgtttgagcc caccgcatcc atgcttgaga aggaaggtgc aggcaaaata
     7081 gtctcttcat tgggagagga aagcggtttg attgcttata ccgcatattt cacatcagaa
     7141 agtgttattg acaagaaccc tgaaatgata cagaagttca caaatgcttt ctataaaggc
     7201 cagctctggg tccacagcca tacagcggaa gagatagccg aggctatcaa atcattcttc
     7261 cctgacgctg acgatgatat cctggttaca gttgtaaaaa gatacaagga aattgatgca
     7321 tggtgcaccg atcccataac aactccggag tcccttgatt tactgcagga agtaatgcaa
     7381 accgcagggg aactggataa gaaagttgaa tatgataaga ttgtaaccca ggaatttgcc
     7441 agaaaggcaa ttgaaacggt aaaaccataa ttattaaaaa acagggccgg agcagaattt
     7501 actccggctt tgcttttttc agccattttt ccatatccaa acaggccttc tggatattat
     7561 ccgtgtttac agtcagttcg tattcaaatt ctttcatttc attctcatac atcggatcat
     7621 aatacctgat catcagatct tccgccacct gatggtaatt cctaagccta atatcccttg
     7681 catactcttc gactctcctt gcagaaatat tccttcccag cttttccaga gagttaatta
     7741 tatccttatc ggaatttttt tctttggtat attcctcaac tatcctccga acccgcattt
     7801 cgatgcttcc aactgcaagt atgtgctttc cttctctcat gctctgcatt atataatgcg
     7861 gtataactat attcccgatt ctgctgcttt ctccctcaac aaatatcatg tcggatttcc
     7921 ttttcttcag gctgtcgtag accagggatt caaactgctt ctggcttatt cgttttccca
     7981 agcccacatc tccgagaaga gaacctctat ggttagcgtg catttcaaaa tccagaatat
     8041 cataacctct gctttcaagc attttcagca atttcgtttt ccctgtcccg gtatagccat
     8101 gtatgacaat atagttcact tctctgttta atttcggcag cattgaattt actacagccc
     8161 tgtagccttt atacccgcct ctcagcttta ctgctccgat accgattgag tttagcaacg
     8221 cacacaggga actgcttcta taccctccgc gctcacagaa cagtattacc ttgtcatggc
     8281 cttccctgag cctttttatt tcttcataca aaagaggaag cttttttgaa gcgaaatcca
     8341 ctcccatttt ttttgcctca tctatgcttc cacagttgta taccgtacct atcagttctc
     8401 tctcttcatc attaaaaatc ggcagattga cagctcctgg tattgtaaat tccgaatact
     8461 ctttcggact ccttacatca acaagcacat aatccccttc aagctgttca taatctatgg
     8521 tgttgtacat tattcccctc acctcgcttc gctaacctcg cggctcgtgc ctcgcaccta
     8581 cgaaatattt taccacaaaa atgtatttat atagacactt aaataaaaag caactgtacc
     8641 atacctgtca atgattcttt ttattcacta ataccact
//
LOCUS       DAOQUR010000081         8449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_9334_length_8449_cov_9.905885, whole genome shotgun sequence.
ACCESSION   DAOQUR010000081 DAOQUR010000000
VERSION     DAOQUR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9334_length_8449_cov_9.905885"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKI50_11380"
     CDS             complement(<1..677)
                     /locus_tag="PKI50_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT04341.1"
                     /translation="MSGHSKWANIKHKKGKADAMRGKIFTKLGKEIAMAAKNGADPST
                     NGKLADVIAKARSNNMPNDTIQRAIKKGSGEMEGVDYEEIVYEGYAPGGVAVIVTALT
                     ENRNRTAGDVRHIFDKNGGSMGVSGAVSWMFDKKGIIIVENDGTTDEEELMMHALEAG
                     AEDFNAEEDSFEIVTSPESFSEVRQKLEEAGIPMASAEVTMVPQNYIEVTDPEAVKKI
                     NKMLDMF"
     gene            complement(756..1493)
                     /gene="nadE"
                     /locus_tag="PKI50_11385"
     CDS             complement(756..1493)
                     /gene="nadE"
                     /locus_tag="PKI50_11385"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNT04342.1"
                     /translation="MINADTVINEAVEWIKNTVEGAGAKGVVFGLSGGIDSAVVAALC
                     KKAYPSDSLGVIMPCYSNPIDEEHAIAVAEAIGLETKTVVLDKAFDSMKAVLGVRESD
                     PRLAVANIKSRLRMITLYYYAGLRNYLVSGTGNRCEFTIGYFTKYGDGGADIMPLTSF
                     VKSEVVELAYKLGIPEAVIIKPPTAGLWENQTDESEMGMTYTELDNYILTGAAEEHIK
                     KKVDAMNKRSEHKRTMIPRFVPSEKQD"
     gene            1735..>2019
                     /locus_tag="PKI50_11390"
     CDS             1735..>2019
                     /locus_tag="PKI50_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT04343.1"
                     /translation="MKNKNHLKTALIGFAAGLVNGIFGSGGGTILVPCLVFLMGIEDH
                     KAHATAISIILPISLISSFIYFRYDVTDLSLTLKVAAGSVLGALFGSFVLN"
     assembly_gap    2020..2119
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2156..2311
                     /locus_tag="PKI50_11395"
     CDS             2156..2311
                     /locus_tag="PKI50_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04344.1"
                     /translation="MKIRKLLAAYTLARGVMKYRRNRQNSPADKFEKCENYYRCDDDE
                     KDGRGNY"
     gene            complement(2312..3202)
                     /locus_tag="PKI50_11400"
     CDS             complement(2312..3202)
                     /locus_tag="PKI50_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT04345.1"
                     /translation="MNRNKVGYVYVLLAAFFFALIAVIGKTVINSGIKIFDLLVLQYT
                     ASFVFMLIYFVIAGIRKLSLPKESLKAVLTQGLIGSSCTTVLFYLALEKLNAGIASML
                     LFTHPVLVSIYFMATKTRKITRRSNLALLTAFFGSIMVINVFNIGIAGTPFIGFVFGI
                     MSSCTYAFYNIYADVKLKDFEPLVIAFYTNLTTLAVSLILHPGFFRFEFLMTSELLIY
                     IFELAVVSGILPVIFLYKGIKLIGADKASIVATSELPITILLSFFVLGERMGLVQLAG
                     ILLIMGSIIILQYEGKLEGG"
     gene            complement(3219..>3788)
                     /locus_tag="PKI50_11405"
     CDS             complement(3219..>3788)
                     /locus_tag="PKI50_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNT04346.1"
                     /translation="FATLDPTTRRVTLGNGKEVLVTDTVGFIRKLPHDLIEAFKSTLE
                     EEVYADLLIHVVDGSNSDYEDQARVVIDVLSELGAGDKASIMAINKIDKCPGRFSPEE
                     YEDMENTVLISASERTGLPKLLALIEGFAAKQSRTVELMIPYAEGSVVSEIYKSADEV
                     TEEQYGEDGIRIKAVVDEISFGRLKKYVI"
     assembly_gap    3789..3888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3889..4564)
                     /locus_tag="PKI50_11410"
     CDS             complement(<3889..4564)
                     /locus_tag="PKI50_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNT04347.1"
                     /translation="MENTIGIKKSLLKRLENLKESTVEKYQLVSEEIIAEICDITEET
                     GKEIAVYIDRKGTVLDVSIGDENTVTLKDFNFRRSEESLSGIRCLHTHPNGHGGLSEV
                     DISALKRMKFDIMAAVGVSNGEMTNATMAFLGVDSDKQFQAVVMGPYSYNRLMKVNIF
                     DIIVEAEAGIKNIRKASKEVDIDAERAVLVGLEDEEALEELGELLKTAGGCEVGRMVQ
                     SRDKKDT"
     gene            complement(4647..5738)
                     /gene="rsgA"
                     /locus_tag="PKI50_11415"
     CDS             complement(4647..5738)
                     /gene="rsgA"
                     /locus_tag="PKI50_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNT04348.1"
                     /translation="MNNLISYGARERYFNEAALYPDLKLARVVSQYKNLYKLVTETGD
                     FLAEISGKFRYEAKGLSEYPAVGDFVMVDLSLGSSSNAIIHRVLTRISAFQRAAAGTE
                     HQTQVVAANIDTVFICMSLNNDYNLNRLERYISMAWNSCATPVVVLTKADLCEDLSVP
                     LSEISAVAPGIDVVVTSSNDRASCDKLLPWLQPGMTASFIGSSGVGKSTLINRLAGEE
                     LLMTSGIRKDDRGRHTTTRRELMILQQGGIVIDTPGMRELGLESVDLSKSFSDIDDLA
                     MKCRFRDCTHTTEPDCAVRKAIEDGTLTERRLQSYHKLKRETCYDGLSFKQIETQKLN
                     AMFGSVGGMKNAKKFIRQNNKREGNTKKL"
     gene            complement(5990..6733)
                     /locus_tag="PKI50_11420"
     CDS             complement(5990..6733)
                     /locus_tag="PKI50_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT04349.1"
                     /translation="MKAFKTLFKTEFKLSLRGMDMFIFAICMPVVAVIILGSIFGNKP
                     AFDGASYSFLEQSFGAVATIAICAGGVMGLPLVISDYRSKKILKRFKVTPISPTVILA
                     VQVVIYAVYSFASLILVYAAAAIFFNYHLNGSWLQFLGAYLIVMVSMFSIGVLVGGIA
                     PNMKMAGIIASLLYFPMLIFSGATLPYEIMPVTLQKAADIMPLTQGIKILKATSLGLP
                     IDSVLAPVIVMIVLAVICTGISIRFFQWE"
     gene            complement(6720..7454)
                     /locus_tag="PKI50_11425"
     CDS             complement(6720..7454)
                     /locus_tag="PKI50_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04350.1"
                     /translation="MESVIKVEKLSKSYSNVTAVKNLDIAVRRGEFFGLLGANGAGKS
                     TTIECILGTVKQDSGVVSILGMNPQKDRKILFERVGVQFQEANYQDKIRVMELCEITA
                     SLYKTPLDYENLLKQFGISEKLKSPVSELSGGQKQRLFIVLALIPNPEVVFLDELTTG
                     LDTRARRDVWNSLSSLKEKGLTILLTSHFMDEVEALCDKIMILKNGKSIFYGTIQEAV
                     ASTPHEKFEDAYLWYTEERGEENEGI"
     gene            complement(7514..8275)
                     /locus_tag="PKI50_11430"
     CDS             complement(7514..8275)
                     /locus_tag="PKI50_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT04351.1"
                     /translation="MNTYKTAEIAKFIGIHPNTVRLYEELGLIPKPERKPNGYRIFTD
                     FHIEQFRFARTALKVEVLQNGLRKQAINIIKTSAAKDFDRAIYLTNCYLKQIKSELQK
                     AEETIAVTEMLLSGEDKASEDMLFTRKEVLNHLLITMDTLRNWEMNGLLTARRKQNGY
                     RVYTSEDIKRLQIIRTLRCANYSLAAILRMMNALSNNPETDIREIIDVPPKDDDIISV
                     CDKLLTSLRYAEKNAFVMLTHLDKMKKLFISNPTL"
BASE COUNT         2360 a         1945 c         1397 g         2547 t
ORIGIN      
        1 tcgaacatat ccagcatctt gtttattttc tttactgctt cagggtctgt aacttcgata
       61 taattctgcg gtaccattgt cacttctgcc gaagccatgg gtattcccgc ttcttcaagc
      121 ttctgcctta cctcggagaa gctttcgggt gaagtaacta tctcaaagga atcctcttcc
      181 gcattgaagt cttcagcccc tgcctccaga gcatgcatca tcagttcttc ctcgtctgtc
      241 gtaccatcgt tttctactat gattataccc tttttatcaa acatccagct tacagcccca
      301 ctaactccca tgctgccgcc attcttgtcg aatatgtgtc tcacgtcccc tgctgtcctg
      361 ttcctatttt ctgtaagtgc tgttactata actgctactc cccccggagc ataaccctcg
      421 tagactattt cttcatagtc cacgccttcc atctcaccgg agcccttctt tatagccctt
      481 tgtatagtat cattaggcat attgttggac cttgccttgg ctataacatc ggcaagcttg
      541 ccgttggtgc ttgggtcggc gccattcttt gcagccatgg caatttcctt gccaagctta
      601 gtgaatatct tacccctcat ggcatccgcc ttgccttttt tatgtttaat atttgcccac
      661 tttgaatgtc cggacatata taaacaacct ccccaatcat agaataaatc gcaattatta
      721 tatcatagaa tctttcacta atgcaaaaaa cttctctaat cttgcttttc agatggtaca
      781 aatcttggta tcatcgttct cttatgctcg cttctcttat tcatggcatc cacctttttc
      841 tttatatgct cctctgcggc tccggtaagt atgtaattgt caagctcagt ataggtcata
      901 cccatctcac tctcatcggt ttgattctcc caaaggcctg ccgtaggcgg ctttataatg
      961 actgcttccg gaatgcccag cttataggcc agctctacta cttcgctttt tacgaaactg
     1021 gtcaaaggca tgatgtctgc tcctccgtcg ccgtattttg taaagtagcc tatagtaaat
     1081 tcacatctgt tccctgttcc ggacacaaga taatttctta atccggcata ataatacaat
     1141 gtaatcattc tgagccttga cttgatattt gcaactgcaa gccttggatc gctttcccgg
     1201 actccaagca cagccttcat agaatcaaag gctttatcaa gaaccaccgt ctttgtttcc
     1261 agtcctatag cttcagctac ggcaattgca tgctcctcat caataggatt gctgtagcag
     1321 ggcattatta ctcccagcga atcggaagga tatgcttttt tgcataatgc agccacaacc
     1381 gcagagtcta tgccccctga aaggccaaat acaacaccct ttgcacctgc tccttcaacg
     1441 gtgtttttta tccattccac tgcttcattt atcactgtat cagcatttat catttgtgta
     1501 cctccaacaa aacaatagtt tttcaatcat tactgtagtt atttcctatc tataaatatt
     1561 ataatagcat tcataaaaaa tgatgtaaat aatagttata gtttagtgca aatatcgtta
     1621 tactccaagt gttccttatt gagaattttt acatgcaata ataaaacgtg taatgcagag
     1681 attaatactg ggaaacaata agtaaaatca aagtcatgat gtatcggagc tattatgaaa
     1741 aataaaaacc atctgaaaac tgcattaatc ggtttcgcag caggtcttgt caatggtata
     1801 ttcggttccg gaggcggtac tattctggtt ccgtgtcttg tattcctcat gggcattgaa
     1861 gatcacaagg cccatgcaac tgcaatttcc ataatacttc ccatatctct tatcagttca
     1921 tttatatatt tcaggtacga tgtgacggat ctttctctta ccctcaaggt tgctgccggt
     1981 tcagtccttg gagcattatt cggctccttt gttttgaacn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnna gagaaaaata aaaagaccgg aaaggagggc tgcagatgaa
     2161 aatcaggaaa ttgctggctg cctacacttt ggcgagaggt gtgatgaaat accgccggaa
     2221 caggcagaac agtcctgccg ataaattcga aaaatgtgaa aactattaca gatgcgatga
     2281 tgatgaaaaa gacggaagag gaaactatta atcagccccc ttccaatttg ccctcatatt
     2341 gaagtattat tattgaaccc attatcagca gtatcccggc aagttgaaca agccccattc
     2401 tttcacccag cacaaaaaat gaaagcaata ttgttatcgg aagctctgag gtcgcaacta
     2461 tgcttgcctt atccgctccg attaatttta tgcctttgta aaggaatata acgggcagta
     2521 tccctgaaac cactgcaagc tcaaaaatat atatcaaaag ctccgaagtc attaaaaact
     2581 caaacctgaa aaaacccgga tgaagtatca atgaaactgc caaagttgtg agatttgtat
     2641 agaaagcaat aactaaaggc tcaaaatctt taagcttcac atcagcatat atattgtaaa
     2701 aagcataggt acagctggac attattccaa acacaaatcc gataaaagga gttcctgcaa
     2761 taccaatatt gaacacatta ataaccataa tacttccaaa aaaagcagtc agtaatgcta
     2821 aattgctcct gcgcgtaatc ttcctggttt ttgtagccat gaagtaaata cttaccagca
     2881 caggatgggt aaacagcagc attgaagcaa ttcccgcatt caatttctcc agtgccagat
     2941 agaaaagaac tgtcgtacac gagcttccaa tcaatccctg tgtcagaacc gcctttaagc
     3001 tttccttcgg gaggctgagc tttcttatac ccgctataac aaaataaata agcataaaaa
     3061 caaatgatgc agtatactgc aatacgagca agtcaaaaat tttgatcccg ctatttatta
     3121 cagtcttgcc gataacagct atcaatgcaa agaaaaatgc tgccaataaa acataaacat
     3181 atcccacttt atttctgttc aaagaataac ccccattact aaataacgta cttttttaat
     3241 cttccgaagc ttatttcatc aaccacagcc tttatacgga ttccatcctc tccgtactgc
     3301 tcctctgtta cttcatcagc acttttatat atttcagaaa ccacactgcc ctcggcgtag
     3361 gggatcatca gttctactgt ccttgactgc ttagctgcaa agccttctat caatgcgagc
     3421 agcttgggca gacctgtcct ctcggaagca gaaataagca ccgtattttc catatcctca
     3481 tactcctccg ggctgaatct gccggggcat ttatcaattt tgtttatagc cataatcgaa
     3541 gctttgtccc ctgctcccaa ctctgaaaga acatcgataa ccacccttgc ctgatcttca
     3601 taatcagaat tgcttccatc cactacatgt atcagaagat ccgcataaac ctcctcttcc
     3661 agagttgact taaaggcttc aatcaaatca tgaggcagct ttcttataaa accgacagtg
     3721 tcagttacca gcacttcctt tccgtttccc agcgttaccc ttctggtagt gggatccaaa
     3781 gtggcaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gtatcctttt
     3901 tatccctgct ctgaaccatc ctgccaacct cgcagcctcc tgccgttttg aggagttctc
     3961 caagctcctc caaagcctcc tcatcctcta atccgacaag cactgctcgt tctgcatcga
     4021 tgtcaacctc tttgctcgct tttctaatat tctttatacc ggcttctgct tcaacaatta
     4081 tgtcaaaaat atttaccttc ataagcctgt tatagctata tggtcccata acaactgcct
     4141 ggaactgttt atcactgtct accccaagaa aagccatagt ggcattggtc atttccccgt
     4201 tggaaactcc tactgccgcc ataatgtcaa acttcattct ttttaaggca gagatatcca
     4261 cctctgaaag tcctccgtgc ccattgggat gggtgtgcag gcatcttatt ccgcttaagc
     4321 tttcttctga ccttctgaaa ttaaaatcct tcagagtaac tgtgttttca tctccgatac
     4381 ttacatcaag aaccgtgcct tttctgtcta tatatactgc tatctccttg ccggtttctt
     4441 ctgtgatatc gcagatttcc gcaattattt cctccgaaac caattgatat ttctcaacag
     4501 ttgattcttt aagattttca agccttttta acaatgactt tttaattcct atcgtgtttt
     4561 ccatataatc acctttgctc cagtagtatg cttttagata ttttaacaca caataatgtt
     4621 caatatgacg aaatacaaaa aatatttcac agctttttag tgttaccctc tcgcttatta
     4681 ttttgacgga taaatttttt tgcatttttc atgccgccga cactcccgaa catggcattg
     4741 agcttttgag tttcaatttg tttgaaggag aggccatcat agcaggtttc ccgttttagc
     4801 ttatgatagc tttggagtcg cctttcagta agtgtcccat cctctatcgc cttcctgacg
     4861 gcacaatccg gttctgtggt atgtgtacag tctcgaaaac gacatttcat tgccaaatcg
     4921 tctatgtcag aaaaggattt gctcaaatcc actgattcca aaccaagttc cctcatgccg
     4981 ggagtgtcaa tgactattcc tccctgctgc aaaatcatca gctctctgcg ggtagttgta
     5041 tgtctgcctc tatcatcttt tctgattccc gaagtcatca aaagctcctc tcccgcaagc
     5101 ctgtttatca gagtagattt ccctactcca gaggaaccta taaaagatgc agtcatccca
     5161 ggctgcagcc aaggcagtag cttatcgcat gaagcccgat cgttgcttga agttactacc
     5221 acatcaatac ccggtgccac agctgatatc tcagacaaag gaacagacaa atcttcacaa
     5281 aggtcagctt ttgttaacac taccaccggt gtagcacagc tgttccaagc catggagata
     5341 tagcgttcaa gtcgattcag attgtagtca ttgttcaagg acatgcatat aaacactgtg
     5401 tcaatgttag cagcaactac ctgtgtctga tgctctgtgc ctgctgctgc acgctgaaag
     5461 gcgcttatcc ttgtcagtac cctatgaata atggcattac tcgaactacc taaactgaga
     5521 tcaaccataa caaaatcacc caccgcagga tattcagata acccctttgc ctcataacgg
     5581 aacttaccgg aaatctcagc caggaaatca cctgtttcag tgacgagctt ataaagattc
     5641 ttatactgcg ataccactct tgcaagcttt aaatcaggat aaagggctgc ctcgttaaaa
     5701 tagcgttccc ttgcaccgta actaattaaa ttattcataa tatttcacct cgttatttga
     5761 gaataaaaag agcatagtca cataactatg ctccgacagt ctctaataaa aaaactgttt
     5821 gtcatagtcc tgtatgaaca tagttgcaca gtaacaaaac gaccgaatat cgccgtttag
     5881 ataatgcaac ttttattcat tacagaacaa tctccacaaa gacacccctt ttattcgttt
     5941 atataattaa attagcacaa tcggttaata gggtcaagta aaaactctgt tactcccatt
     6001 gaaagaagcg aatagaaatg cctgtgcaaa ttactgcaag cacaatcatc acaataacag
     6061 gggccagaac actgtctatt ggtaaaccaa gcgaagttgc tttgaggatt tttatcccct
     6121 gtgtaagcgg cattatgtct gctgcttttt gaagagtcac aggcatgatt tcataaggca
     6181 aagtagcacc tgagaaaatg agcatcggaa agtatagcag gcttgctatt atacccgcca
     6241 tcttcatgtt tggcgctatt cctccgacaa gtactcctat actgaacata gacaccataa
     6301 ctatcaggta tgctcctaaa aactgtaacc atgaaccgtt taaatggtag ttgaaaaaga
     6361 tcgctgcagc agcataaact aaaattagtg aagcgaaaga ataaacagca tatataacaa
     6421 cctgtactgc taaaatgact gtagggctga ttggagtaac cttgaaccgc tttaagattt
     6481 tcttgctacg ataatcggag ataactaagg gaagccccat cacacctccg gcgcatatgg
     6541 ctattgttgc tactgcacca aaggattgtt ccaagaagct atagcttgcg ccatcgaagg
     6601 caggcttgtt tccaaaaatc gagcctaaaa taattactgc tacgacaggc atgcaaattg
     6661 caaaaataaa catgtccatc cctcgaaggg acaatttgaa ttctgtttta aaaagtgttt
     6721 taaatgcctt cattttcttc acctctctct tctgtatacc aaagatacgc atcttcaaat
     6781 ttttcatgtg gagttgaagc aacagcttcc tgtatagttc cgtaaaaaat gcttttcccg
     6841 ttttttaaaa tcataatttt atcgcaaaga gcttccacct catccatgaa atgagatgtc
     6901 aataaaatag tcagcccttt ctctttcagg ctggacaaac tattccatac atcccgcctt
     6961 gctcgggtgt caagtccggt ggttaactca tctaaaaaca caacctcggg atttgggatt
     7021 aaagccaaaa caataaacaa tcgctgtttt tggcctccgg aaagctcact tacagggctt
     7081 ttcaacttct ctgaaatccc gaattgtttt agcaagtttt cataatccaa aggagtttta
     7141 taaagagacg ccgtgatctc acatagctcc ataactctta ttttatcttg gtaatttgct
     7201 tcttgaaatt gaactccaac cctttcaaat agtatttttc gatctttctg cggattcatt
     7261 cctaaaattg atactactcc gctgtcttgt tttacagtgc ctaaaataca ctcaatggta
     7321 gtgcttttgc ctgcaccgtt tgcgcccaac aatccgaaga attctcctcg tctaacagca
     7381 atatccaaat tcttaaccgc tgtaacatta gagtaagact tgcttaactt ctctactttg
     7441 attactgatt ccattcgatc aaatccctcc attcttttct aaaaaaagaa taacacctga
     7501 gaaaactctg gtgttatagt gtagggtttg aaataaaaag ttttttcatt ttatcaaggt
     7561 gagtcagcat aacaaaggca tttttctcag catacctcaa ggatgtaagc agcttgtcac
     7621 atacagaaat aatgtcatcg tcctttggag gcacatcaat tatctcccgt atatcagtct
     7681 cagggttatt ggataaagca ttcatcatcc ttaaaattgc tgcaagagaa taatttgcac
     7741 agcgtaaagt gcgtataatt tgcagtcgtt taatgtcttc agaagtatat actcgatagc
     7801 cattttgttt tcttcttgct gtcaacaatc cgttcatttc ccaatttctc agagtatcca
     7861 tagtgattag cagatgatta aggacttctt ttcgcgtaaa aagcatatcc tctgaggctt
     7921 tatcctcccc tgataataac atttcggtaa ctgctatggt ttcttcggct ttttgcagtt
     7981 cactttttat ttgcttcaaa taacaatttg taagataaat tgctctatca aaatctttgg
     8041 cagcagaggt ttttatgata ttaatagcct gcttcctgag accattttgc agaacctcga
     8101 cttttagagc agttctcgca aatctaaatt gttcaatatg aaaatcagta aagatccgat
     8161 aaccatttgg tttccgttca ggtttgggaa taagtcccaa ttcttcataa agtctaactg
     8221 tgttaggatg aataccaatg aatttcgcta tttctgcagt tttataggtg ttcatgctat
     8281 cagagcacct ccgttctaat atattatagc attattgtta catataagtc tgggttattc
     8341 tataaatgca tgtgccaacg ggtaaaaaaa taacagcctc agctgttatt tttattacct
     8401 ggcggcgatc tactcttccg ggaccctgcg gtccgagtac catcgacac
//
LOCUS       DAOQUR010000082         8183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_9703_length_8183_cov_11.284449, whole genome shotgun sequence.
ACCESSION   DAOQUR010000082 DAOQUR010000000
VERSION     DAOQUR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9703_length_8183_cov_11.284449"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            680..1441
                     /locus_tag="PKI50_11435"
     CDS             680..1441
                     /locus_tag="PKI50_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT04352.1"
                     /translation="MPCQHLEHCPAPAEQNRYIVQEGDTLYSISRFYNISLDDLIEAN
                     PNLEPDRLLPGLEICIPLAAQPADCPFGATTYTVQRNDTFYSIAKKFKMRLSELLKSN
                     PDLNPDALLIGQIICLPKISSSYSNEAYRVRFSYPYLWSRFDSKRHEGIDGFFQISAI
                     SDDAAPEEICKKEAYHKLKPYGTHPTISRTELRGRQAFFIIPSSDQPKEMRGQSAMIV
                     ECSEPVEIEGNNCRYFILRTDKEHLHDIADTLEFF"
     gene            complement(1438..3477)
                     /locus_tag="PKI50_11440"
     CDS             complement(1438..3477)
                     /locus_tag="PKI50_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT04353.1"
                     /translation="MKKRAISYFLFLLMTAFVLNACLPAFAVENEDTYDDAPLLDKQT
                     AERELLFFEDNSYNFKDIVKHWARDEMLELSYMDILKGYSSTTMLPEKSISREEYIAM
                     LVRAIGIPTDDEFVESYTDVTDKHWSHKYVTAAKEHGLLSVFSGYYFYPYREITREEM
                     AVITAKAVQNADINGEKLSFKDIPQYYRHKESIDLVTALGIIKGLPDGSFGPKGKATR
                     AQAAAVIGRVLRTREASGEAEDEYLKAFAADYERTVLESLSEGDLGFDTPISLSAGKE
                     ARLNAKRSEQLKIQYPKGFTRSKHMVNESFAVLSKSRYLAEVEAAYELVLENGDRYSV
                     RKSIFLKNIGDKWAVYNSIPEFGADFASGTKINLTWHYIWKDTPDMSSVKKIEGLNVV
                     SPTWFTLANEKGDLEDRGSLSYSNWAHKNGYKVWALVDNQFNSDLTSKLLGNASARAK
                     FISSLISCAKKYKLDGLNIDFENMYIKDKNAFTQFVKELSIQAKADNLVLSVDVSIIA
                     VNSNWSESFDRAALSGYVDYVALMTYDQHWGGSPISGSVAQLSWVERSLKRVLEEVPN
                     EKLLLGVPFYTRLWKEEYVSGSSKPVITSKAISMEEAEKTVAENKAAKIWDPVSGQYY
                     AEYKKGNATYKIWLEDEKSIKLRAELVNKYRLAGIASWKYGLEKPVIWDVIAKTI"
     gene            complement(3694..4230)
                     /locus_tag="PKI50_11445"
     CDS             complement(3694..4230)
                     /locus_tag="PKI50_11445"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNT04354.1"
                     /translation="METKNSNYSSTSLKIRDLVQISLLSALTFVAAWAIHIPYGNGGV
                     IHLGDSMIFLTAVVFGRRYAAISGAIGMTMFDIFSGFSVWAPYTFVIKAVMGLIAGSL
                     ANLGDSKGSSAVKNGLGMLAAGIWMIFGYYAAEAIITWNITQPLFSLLGNVIQAGAGA
                     AIAFVIIAAIKRSGYFNK"
     gene            complement(4341..4988)
                     /locus_tag="PKI50_11450"
     CDS             complement(4341..4988)
                     /locus_tag="PKI50_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04355.1"
                     /translation="MRKSADGKGNLKIADDSKTPSEIYSIVLRGVISTLRTIFLNYYV
                     SENIEALMRYINEKKIISSAEYKRLLYEAKDFYPVIVCALFINPLKKHTKNFKSVIID
                     NQSKKALFNEINEELNKFFNDWLCDVEIDPLLFFRLSAAYGKPFSLDLAIKQQLAAVL
                     TPIEERILKLQITEIVALYTIEQVATHLGLAGLDYIDYVKVEKKEEIVSIQSSRN"
     gene            5222..5968
                     /gene="rluF"
                     /locus_tag="PKI50_11455"
     CDS             5222..5968
                     /gene="rluF"
                     /locus_tag="PKI50_11455"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HNT04356.1"
                     /translation="MKNSDHDDSIRINKHISEKGLCSRREADELIRQGRVTINGKKAS
                     IGSKVYPGDIVKLGGKTLSPKAPPVYIAFNKPPGIVCTTDRREKDNIIDFINYPKRIF
                     PVGRLDKESEGLIFLTSDGDIVNKILRAGNNHEKEYVVTVDKPVTEDFIESMSKGVRI
                     LGTVTKKCRVSRISGYVFRIILTEGMNRQIRRMCEVFGYEVVKLRRVRIMNVNLGSLP
                     EGRWRYLTSHELNTINKLVAESVKTEEASR"
     gene            6065..>8183
                     /locus_tag="PKI50_11460"
     CDS             6065..>8183
                     /locus_tag="PKI50_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNT04357.1"
                     /translation="MKRMVSIITLAAVLLGILLAGPVAAAENGNLDLKEALEIAKIAF
                     DFDTANHDFSSSYSETKFGRKLWYLNWNSNDGRGSSMSVTVDAATGEIINMYQWKNMP
                     QPLNRIPKHTREEALEVAEALAGRLHPDKFKDTELMNEYWDNIYRSYYSSDSYNFNFI
                     RKIKGIDFQDNMIQVQVDKNTLEVRSFNLDWDTVIPIPDSAKAISADAAKKIFEEKLG
                     IELAYQMIYPNNSRDSKLILVYNQKNANHRIDAITGEIMTQPYYGLMEKSAMGGGDAG
                     AAGVAITPEEQKVIDDSSKYISKEKALESFTKLVPVDSRYKMDNSSLYGGYNNENAAW
                     SFSWSYNDSANNTYSYIYGTVDAVTGEVKSFSISNSENEYKAGRAQKYTKEQCREIAE
                     KFLKEIQPDKFSTSEYRELYFEIYADPQNVPSYNMNYIRKENGISVPFNNLSVVVNTY
                     TGEITNFSINWQDLDFPDTAGVISLEDAYKLLYAKHDLMLKYIRIYNYNRFEDNTEIK
                     LAYMPDDYYGMIDAKSGQFIDYNGNPVKEFKEISFTDIEGHKFENDIKLLVELGIIDS
                     AESKFNPDTRITQKEFVKLLMKATQPDYYPIPYAASDSSEYDRYYENAINQNILSRNE
                     KNPEACVTRMEASRMTVKALGVGFVADLGSIFNLNLKDAASITAENKGYAAIAAALGI
                     VDTSAGSFVPGLELTKAETAGMLI"
BASE COUNT         2402 a         1836 c         1698 g         2247 t
ORIGIN      
        1 tgaggtcaat agattttcga gcaagctgat ttgatttgtg gcaaggcggg cgctcttgta
       61 atgcttatcg ccttacaaga gcggacaacg cagctacaag gtaaatcagc tgctctaaaa
      121 cggattcgta tcagtttgtt ttgcttagcc tttggaaaag tcctaacggt gccaaaggcc
      181 atccgtgacc cgtggtacct aagccggcgt ccttgccggc tttacggact ttttaccaaa
      241 gccatatcag aggcctgtta tcaaaatgca taaaagtgtg ccctgctata ctatacaagc
      301 tcttgcttag tatgcatcct acagagcatc atatgttata caaggccagt gccctttggg
      361 aattatgctg tattaaggaa atgcaaatac cgacgtgggg aagatatctt tgacgaaact
      421 ttccacctga cctcggaacg ggaatacccc aggcaaaata accagacggg ctcgcagaga
      481 tgggacccac gccagtgatt ttgctggctt tggaaagtga ggagaacata cttccccaac
      541 agtcggagta cttcacatac tattgtaatg atgtcgcact attcttctat tgtgcacaag
      601 atatattgta accttcaaat gaatatatta tattacccgg catttttagg gtatataata
      661 tttcaaagag gtgagcgtaa tgccttgtca gcatttggag cattgcccgg cccccgccga
      721 gcaaaaccgg tacatagtgc aggaaggaga tactctatat tcaatatcaa gattctacaa
      781 tatttccctg gatgatttga tagaagccaa tcctaatctg gagcccgacc gtttgctgcc
      841 gggactggaa atatgcatac cactggcggc gcagcctgca gactgtccct ttggggccac
      901 aacctacacc gtgcaaagaa acgatacctt ttattccatt gccaaaaagt ttaaaatgcg
      961 attgtcggaa ttgcttaaat caaatcccga tctcaatcct gatgcgctgc tgatcgggca
     1021 aataatatgc ctgcctaaga tatcatcaag ctattccaat gaggcctata gggtcaggtt
     1081 cagctaccct tacctctgga gcaggtttga cagcaagcgg catgaaggaa tagacggatt
     1141 ctttcagata tctgccattt ccgatgatgc agcaccggaa gagatatgta aaaaagaagc
     1201 ataccataag ctgaaacctt acggtacgca tccgacaata agcagaactg agttacgcgg
     1261 ccggcaagcc ttctttatta taccctcttc cgaccagccc aaggagatgc ggggccagtc
     1321 tgctatgata gttgagtgtt cggaaccggt cgagattgaa ggcaataatt gcaggtactt
     1381 cattctccgg acagacaagg agcatctgca cgatattgcc gatacattgg aatttttcta
     1441 gattgtcttt gctattacat cccatattac gggcttttca aggccatatt tccatgaagc
     1501 tatacccgca agcctgtact tgttcactaa ttcagccctc agctttatgg atttttcgtc
     1561 ttccagccat atcttatagg ttgcattgcc cttcttgtat tccgcatagt attgaccgct
     1621 tacaggatcc caaatttttg cagctttgtt ttctgcgacg gttttttccg cttcctccat
     1681 ggaaattgcc ttggaggtta taaccggttt gctgcttccg ctcacgtact cttccttcca
     1741 gagccttgta tagaagggca cccccaacag aagcttttca ttcggtactt cctcaagtac
     1801 cctcttcagg ctccgttcaa cccaggacag ctgtgcaaca gagccgctta tggggcttcc
     1861 tccccaatgc tggtcatagg tcattagcgc cacataatca acataccccg acagtgcagc
     1921 cctgtcaaag ctttcagacc agttggagtt tacggcaatt attgacacgt caacagacaa
     1981 caccagatta tcggcttttg cctgtatgga aagctcttta acaaactgtg taaaagcatt
     2041 cttatccttt atatacatat tttcaaaatc gatatttaat ccgtcaagct tatatttctt
     2101 tgcacagctt ataagggaac ttatgaattt tgctcttgcg cttgcatttc caagcagttt
     2161 gcttgtcaaa tcggagttaa actgattgtc taccaaagcc cagaccttat acccgttttt
     2221 atgggcccaa ttgctgtagc tcaagcttcc cctgtcttcc agatcgcctt tttcgtttgc
     2281 cagtgtaaac catgttggtg agactacatt aaggccctca attttcttga cacttgacat
     2341 atcgggggta tctttccaga tataatgcca ggtaagattt atctttgttc ccgaggcaaa
     2401 atctgcgccg aattccggaa tgctgttata tactgcccac ttgtctccta tgttcttcaa
     2461 aaatatgctt ttcctgactg aatacctgtc cccgttctca aggaccagtt cataagcagc
     2521 ttcaacttca gccaggtacc tggatttgct taataccgca aagctctcat tgaccatatg
     2581 cttgctgcgg gtaaacccct tgggatattg tatttttagc tgttcagacc tcttggcatt
     2641 aagtctagct tcttttcccg ctgaaaggga tatgggagta tcaaagccca aatcgccttc
     2701 gctcagggat tccaaaacag ttctttcata atctgccgca aaagccttta ggtattcatc
     2761 ttcagcctct cccgaagcct ccctggttct cagtactctt cctattaccg ccgcggcttg
     2821 tgctcttgtt gcttttcctt tagggccgaa gctcccgtcg ggcaacccct tgattattcc
     2881 caatgccgtt accaggtcta tactctcctt atgcctgtaa tattgcggaa tatccttgaa
     2941 gctaagcttt tctccgttaa tgtcagcatt ttgtacggct tttgccgtga ttaccgccat
     3001 ttcctccctg gttatttccc tgtagggata aaaataataa ccgctgaaaa cagacagcaa
     3061 accatgctcc ttggccgctg ttacatactt atggctccag tgcttgtccg taacatcggt
     3121 gtagctttcg acaaactcat catcagtagg aattcctatt gctcttacaa gcatagctat
     3181 atactcttcc ctggatatgc tcttttccgg cagcattgtg gtactgctgt aacccttcaa
     3241 aatatccata tagctcagtt caagcatttc gtcccttgcc cagtgtttga ctatatcctt
     3301 aaaattatag gaattgtcct caaaaaaaag caattctctt tctgcagtct gcttgtccaa
     3361 aagaggtgca tcatcatatg tatcctcatt ttcgacagca aaagcaggta aacaggcatt
     3421 caacacaaaa gctgtcatta ataaaaataa aaaataactt atggctcttt tcttcataac
     3481 ttcacactcc ccataatatt atatcaatta tgtaaactag atttgttaca gggagatttc
     3541 aaaaaacttg tattaaatag aaaaagagac agggggagac atagggacac ggggacgctt
     3601 cgggacacgg ggacgtttct cctgtcccgc tttcagcggg acaggagaaa cgtccccgtg
     3661 tcccgaagcg tccccgtgtc ttgccagaac ctgttattta ttgaaatagc cgctcctttt
     3721 tatggctgct attattacaa aggctattgc cgcaccggca ccggcctgta tgacattgcc
     3781 caataggctg aataacggct gtgtgatatt ccatgttata attgcttccg cagcataata
     3841 tccgaatatc atccaaattc ctgccgccag cattcccaat ccattcttaa ccgcactgct
     3901 gccctttgaa tctccaaggt tcgccaggct gccggcaatc aaacccatta ccgctttaat
     3961 cacaaaagta tatggtgccc atacggagaa acccgaaaat atatcaaaca tagtcatacc
     4021 tattgctccg gatattgcag catatcttct gccaaagacc accgccgtaa ggaatatcat
     4081 actgtctccc agatgtatga ctccgccgtt tccgtagggt atgtgaatag cccaggcagc
     4141 tacaaaagtc aatgcggata aaaggcttat ctgtactaaa tctcttattt tcaaactggt
     4201 gctgctgtaa tttgaatttt ttgtttccat tttgccgact cccccaaaat tatatattgc
     4261 tgttgctgct tctctgattt cattatacag gagccgctgg agggaacaat acggaatcag
     4321 caattttatg cacagtttat ttagtttctg cttgattgta tcgatacaat ctcttctttc
     4381 ttttccacct ttacataatc aatataatca agccctgcaa ggcccaaatg tgttgcaacc
     4441 tgctctatgg tatacaaagc aacgatttct gttatttgga gctttagaat cctttcttct
     4501 attggtgtaa gcacggctgc aagctgttgt tttattgcaa gatcaaggct gaaaggtttt
     4561 ccataggctg cggaaagccg aaaaaacagc agcggatcta tctccacatc acagagccaa
     4621 tcattgaaaa atttatttaa ttcttcatta atctcattga acagcgcctt tttggactga
     4681 ttatcaataa ttacgctctt gaaattcttc gtatgctttt ttaaaggatt aatgaacaat
     4741 gcgcaaacaa tgacgggata aaaatccttt gcctcatata aaagcctttt atactctgca
     4801 gagcttatta tcttcttttc attaatatat ctcatcagtg cctcgatatt ttccgagaca
     4861 taatagttca aaaaaattgt cctcaaggta ctgataacgc cccgcaatac aatgctgtaa
     4921 atctctgaag gagtcttact gtcgtctgca attttcagat ttcctttgcc gtctgcactt
     4981 tttctcatgt tcacacctcc acaagacttt caaggaaatt taatgaaccc ctgagccctt
     5041 tttcctctat atctaattat ttacaaattc tttttatcta ttcagcaatt tgatatacat
     5101 taattgatta atttaccgta tataagtaaa atataaatac aggcacggag ctgcggttca
     5161 gggatacccg gtgattgccc cctggtggct gccgtgcaaa aagttgaaag ggcattttat
     5221 tatgaaaaac tcagatcatg atgatagtat ccgaatcaac aaacacataa gcgagaaggg
     5281 cttgtgctcc cgcagggagg ctgatgagct tatcaggcag ggaagggtca caataaacgg
     5341 caagaaggct tccattggca gcaaggtata tcccggggat attgtaaagc tcggaggaaa
     5401 aaccctctct cccaaagctc ctccggtcta cattgccttt aataaacctc ccggaattgt
     5461 atgtactacg gacagaaggg aaaaagacaa tattatagac tttatcaact accctaaaag
     5521 aatattcccc gtaggaaggc ttgataaaga atcggaaggc ctgattttcc tcacaagtga
     5581 cggtgatatt gtaaataaga tactccgggc aggcaacaat cacgagaaag aatacgtagt
     5641 gacggtagac aaacctgtta ctgaagattt tatcgaatcc atgtcaaagg gtgtacggat
     5701 tctgggaact gtaacaaaaa agtgcagggt atcccggata tccgggtatg tgttcaggat
     5761 aatacttacg gaaggaatga acaggcagat cagaaggatg tgtgaggtgt tcggatatga
     5821 agttgtgaag ctgaggcggg tacggattat gaacgttaat ctgggttcac ttccggaagg
     5881 aagatggaga tatcttacaa gccacgagct taatacaatt aacaagcttg ttgcagaatc
     5941 agtgaagacc gaagaggcct ccagataaat gttcctccgg aatcatcgtt ttgccggaac
     6001 ttttattaat tattggcagt caaagttgta tacattcaaa taagctatga aagggagata
     6061 aattatgaaa aggatggtat ccattattac actggctgca gtactcttag gcatcctgct
     6121 tgcagggccg gtagccgctg cagagaatgg taatctggat cttaaagagg cattggaaat
     6181 tgccaaaata gcttttgact ttgataccgc aaatcatgac ttcagcagca gctattcaga
     6241 aacaaaattc ggaagaaagc tgtggtatct gaactggaat tccaatgatg gaagaggctc
     6301 aagcatgagt gttaccgttg atgcggcaac aggggaaata ataaacatgt accaatggaa
     6361 gaacatgccg cagcctttaa acagaattcc aaaacacacc agggaagaag ctttggaggt
     6421 agccgaggct ctggccggaa ggcttcatcc tgacaaattc aaagatacgg agcttatgaa
     6481 cgaatactgg gacaatatct atcgatccta ttacagcagc gattcataca acttcaattt
     6541 cataagaaag ataaagggaa tagactttca ggataatatg atacaggtac aggtagacaa
     6601 aaacaccctc gaggtaagaa gcttcaacct ggattgggat acggtaatac ctattcccga
     6661 ctctgcaaaa gcaataagcg cggatgcggc gaagaagata tttgaagaaa agctcggtat
     6721 tgagctggca tatcagatga tatatccgaa caattcaagg gattccaaac tgatactggt
     6781 atacaatcag aagaatgcaa accatcgtat tgatgccatt accggcgaga taatgaccca
     6841 gccttattac ggattaatgg aaaagtccgc catgggcggc ggcgatgccg gtgcggcagg
     6901 tgttgcgatt actcccgagg agcagaaggt gattgacgat tccagcaagt atataagcaa
     6961 ggaaaaagcc ctggaatctt ttacaaaact tgtacctgtc gacagcaggt ataaaatgga
     7021 taactcaagc ctctacggcg gttataacaa tgaaaacgca gcctggagct tcagctggag
     7081 ctataatgac agtgcgaaca atacttacag ctatatatac ggcacggtag atgctgttac
     7141 cggtgaagtc aaaagcttta gcatcagcaa cagcgagaat gaatacaaag ccggacgcgc
     7201 tcaaaagtat acaaaggaac aatgcaggga aatagcggaa aaattcctta aagaaataca
     7261 gcctgacaaa ttcagcacct ctgaatacag ggagctgtac tttgaaatat atgcagatcc
     7321 tcagaatgta cccagctaca atatgaacta tatcaggaag gagaacggca tctctgttcc
     7381 cttcaacaat ttgagcgtag tggtaaacac atataccgga gaaataacaa acttctccat
     7441 aaactggcag gacctggact tccctgacac agcgggtgta ataagcctgg aagacgctta
     7501 taagctgctg tatgcaaagc atgaccttat gcttaagtat ataaggatat ataattataa
     7561 caggtttgaa gacaatacgg aaataaagct tgcttacatg ccggatgact attacggcat
     7621 gattgatgcc aagagcggcc agtttataga ctataacgga aatccggtaa aagaattcaa
     7681 ggagatatcc ttcaccgaca tagaagggca taaatttgaa aacgatatca agcttcttgt
     7741 ggagctggga attattgatt ccgccgaaag caagttcaat ccggatacaa gaataaccca
     7801 gaaagaattt gttaagcttc tgatgaaagc aacccagccg gattactatc ctatccccta
     7861 tgcagcaagc gacagcagcg aatacgacag gtactatgaa aatgccataa atcagaacat
     7921 actgtccaga aatgaaaaaa atcccgaagc ctgcgtcaca agaatggaag cgtcaagaat
     7981 gacagtaaaa gcattgggtg taggctttgt tgcggacttg ggaagtatat tcaacctcaa
     8041 tctcaaggat gccgcaagta ttaccgcaga aaacaaaggc tatgccgcca ttgcagcagc
     8101 tttaggaatc gttgacactt ctgcaggcag cttcgtaccg ggtctggagc ttacaaaagc
     8161 tgaaacagca gggatgctca taa
//
LOCUS       DAOQUR010000083         7818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_10278_length_7818_cov_10.296406, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000083 DAOQUR010000000
VERSION     DAOQUR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10278_length_7818_cov_10.296406"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..731)
                     /locus_tag="PKI50_11465"
     CDS             complement(264..731)
                     /locus_tag="PKI50_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04358.1"
                     /translation="MLQFDGKEIKDDNYDRYIYMSINNEQGQKMYDRAEKLRSNTPDY
                     NLYEFNCNHMAQTILLEADLSFSKMRGNASDEKELLDAPYKIGVWDIISAYRKDREDL
                     TIPNAAYKHGVSIAEDNGWIIGETDWKHETIRTRRGSKEYQHEHNPLEYNPFR"
     gene            complement(764..1363)
                     /locus_tag="PKI50_11470"
     CDS             complement(764..1363)
                     /locus_tag="PKI50_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04359.1"
                     /translation="MAELELEIEKFSEIDGIGAEELKDMLIPKLGTTLSRETKGYNVY
                     VDLMQHKLNKLGYKDMEGKELKEDGVFGTNTEFAVNTFKETNKLWNSKEYRGKVGQTS
                     YDMLFSGKAKRAPEYVDQGTGDDDISFKAVIYLNRSNRANLQGHAAIMLVTEDNRGLF
                     YSFTGDDTDVFKVLNGENVSGALLTVVDPETKNKHSFRM"
     gene            complement(1711..2100)
                     /locus_tag="PKI50_11475"
     CDS             complement(1711..2100)
                     /locus_tag="PKI50_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNT04360.1"
                     /translation="MESKTPVNESLETLFSHLEKFLKTETVIGEPIVVGETTLIPIIT
                     VAFGCGGGGGGGKDEKGNDGMGTGVGVGAKISPDAVIVIKQDSVTMLPVKNRSNIEKL
                     VGMVPEIVKKIKLKKEEKSCCAEEKSE"
     gene            complement(2171..2827)
                     /locus_tag="PKI50_11480"
     CDS             complement(2171..2827)
                     /locus_tag="PKI50_11480"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HNT04361.1"
                     /translation="MVVLLIIVKILLYLLLFLLGLVFLLLIIPVNYRGQVLTAEGFRA
                     QFAFGWAGKLFGISAEMEGENLDVTLRILDKRVCKLKSKGQEGKKEQSEEKTEKKEKE
                     KNVPRFREITDRTLINEILEYIKKVLNIVKPKYMHLYATYGFDDPSLTGMVCGAAAVV
                     KSMIPHARVGLYPDFTQEIIDLDLRAEGSMTVGSLAYQTIRTALKKPVRKILFRKKKS
                     "
     gene            complement(2850..3284)
                     /locus_tag="PKI50_11485"
     CDS             complement(2850..3284)
                     /locus_tag="PKI50_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04362.1"
                     /translation="MNCGFDREIIQRYADNTIDPLEFIFLKEHINYCGECRKELDMVM
                     TLENELYKFFDDDSEIRNPDMNIARLVDDCMYGLNKREKLKYTLTRSIKFGKKVADNS
                     LKFAGYIPGSKIICKGAKRTASYTGNLIKSLVKKEVKKLLAR"
     gene            complement(3302..3862)
                     /locus_tag="PKI50_11490"
     CDS             complement(3302..3862)
                     /locus_tag="PKI50_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT04363.1"
                     /translation="MNVEAGIIVRCKKYDKSAFTELFKMYEKYLYKLCYNYVQNEQDA
                     LDIAQEVYIKVFNNISSFDVKMPFHPWFRTIAVNTCLNFKRTIRYDSVSLNAGYEDDR
                     TIEEVVAASKDVESEVLDNELGHIIRENMECLRPKHRMVLILRYYEGLSYEEIAAVLK
                     EPLGTVKTDIYRARNILKEKLRNAMK"
     gene            4143..4562
                     /locus_tag="PKI50_11495"
     CDS             4143..4562
                     /locus_tag="PKI50_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT04364.1"
                     /translation="MKVKQIMTTDVSTVTPNDTVTKAASIMKQLNVGSVPVTDGSRVV
                     GIVTDRDITLRGVAKSGDPNQKISEVMTTDVKYATPDMDVHAVADLMAENQVRRLPVI
                     DNDRLVGIVAIGDLAVENIFENEAGEALHNISMGVRH"
     assembly_gap    4718..4817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4849..5535
                     /locus_tag="PKI50_11500"
     CDS             4849..5535
                     /locus_tag="PKI50_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNT04365.1"
                     /translation="MLIVFVRTLILYILIIVVLRLMGKRQVGQLQPSELVVALIIADL
                     AAVPMSEVGIPLINGIIPIITLFIMEELLSYISMKSERARGLISGKPSILIERGTIIE
                     DELRRIRYNINDLLEQLRLKNFSNVEDVSYAILETSGQLSVIPKEEKKPVTLKDMNLI
                     AKTGHLPVTVIIDGRIISDNLYKIGLSNNWLADQLRKNSIKSSEDVFFAYLNPERKFI
                     YQLRSNSQKK"
     gene            5536..5940
                     /locus_tag="PKI50_11505"
     CDS             5536..5940
                     /locus_tag="PKI50_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HNT04366.1"
                     /translation="MQKGAADELKPLIIIISLTILIVAGGSITLYLLNSESQRLDDSL
                     SSLGEAIENGNWDNASKNLKEFNSKWDRTSSLWSMLVDHYEIDNIELLLSQLASYVKN
                     KNRNDALSSVSSLKTLIKHIPEKESLSLKNVF"
     gene            complement(5924..6814)
                     /locus_tag="PKI50_11510"
     CDS             complement(5924..6814)
                     /locus_tag="PKI50_11510"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT04367.1"
                     /translation="MKSYNTYIIDKENDGITLGVYLRSRLMMSRNGLIKVKKSGSLKV
                     NGFKAHTDIIIKAGDKVEFELPDRNSDNILPEFMPLDIIFEDDYLIVINKEAGIPTHP
                     SRNHYMGTLANGLMYHWMEESRDITIRPVNRLDKNTSGLVIFAKSSHIQHLMSAEGYR
                     SNITKEYLAVVQGVVEADSGTIDAPIAREKLHSIRRVVREDGAEAVTHYSVVERHEDC
                     SLLKIVPETGRTHQIRVHMAHLGHHLLGDELYGGRLEKIKRHALHAYKIKMLHPIENR
                     MLDFTAQVPSDMEELIKKHF"
     gene            complement(6822..7712)
                     /locus_tag="PKI50_11515"
     CDS             complement(6822..7712)
                     /locus_tag="PKI50_11515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT04368.1"
                     /translation="MQKKYPVRLSENRIFVDAYMSQQLGFSSRQIKKLFKDKKVCING
                     KTAYRDNKLKDGDIIEVDLSVEAREDIVPENIKLDIIYEDEYLLAVNKPPYMLVHPTP
                     NHPGGTLLNAAAYYFAGKGEEVSLRLLNRLDMNTSGIVVMPKNGLVHGRLDEMMRSGG
                     VRKFYTAVIEGIAAPEKGVIDRPIGKDESDPIKRKVKSDGQPAVTKYDTLKTGKSHSL
                     VRLELVTGRTHQIRVHLSFLGHPIVGDTLYGKKSCLIGRQALHASDMELAHPVTGNLL
                     KLHAEFPEDIRLLASRLELE"
BASE COUNT         2047 a         1808 c         1346 g         2517 t
ORIGIN      
        1 cctttgttct catctatcga tttatagccc agtattttag tcttgtcagc attaatggct
       61 acttcattat tgaaattacc tgctaataca ctcttaaact tattctccgc tggagaataa
      121 gcagtaatcc cctctctact attcagcata atttctccta caagattgct cttatttaag
      181 ctacagcctg acattattaa atataaacca atacatgaga taataatcat ccattttctc
      241 ataaaaaaca tcacctctca caattatctg aatggattat actctagagg gttatgttca
      301 tgctgatact cctttgaccc tcttctcgtt ctaattgtct catgtttcca atccgtttct
      361 cctataatcc atccattgtc ttctgctatc gacactccat gtttatatgc agcattcggg
      421 attgttaaat cttccctatc ctttctatat gcagaaatta tgtcccaaac acctatctta
      481 tatggtgcat ctagcaattc tttttcatca cttgcatttc ctctcatttt tgaaaaactc
      541 agatcagctt ctaacaaaat cgtttgcgcc atatggttac aattaaactc ataaaggtta
      601 tagtcaggtg tattactcct tagcttttca gctctatcat acattttctg gccttgttca
      661 ttattaattg acatgtagat atatctgtca tagttatcat cttttatttc cttcccatca
      721 aactgcagca catttttaat attgccagtc ttcagaaatt catttacatc cggaatgaat
      781 gcttgttttt tgtttctggg tctacaacgg ttaacaatgc accactaacg ttttcgccat
      841 taagtacttt aaaaacatct gtgtcatctc cagtaaagct ataaaacaag cctctattat
      901 cctcagttac aagcattata gcagcatgtc cttgtaaatt cgctctgtta ctcctattta
      961 gataaataac agctttaaaa gagatatcat catcacccgt cccctgatca acatactccg
     1021 gtgccctttt tgccttaccg ctgaacagca tgtcgtagct tgtctgtccg accttccctc
     1081 tgtattcctt gctgttccac agcttgttcg tttctttgaa tgtgtttact gcgaattctg
     1141 tgttcgtgcc gaatacgccg tcctccttaa gctccttgcc ttccatatcc ttatagccaa
     1201 gcttgttcag cttatgctgc atcaagtcga cgtacacgtt ataccctttt gtttctctgg
     1261 ataatgtagt gccaagcttc gggataagca tgtccttaag ttcttcggcc cctattccgt
     1321 ctatttcgct gaatttctct atttccaatt ccagctcagc cattcgcttc ttcagctgtt
     1381 ctgccttgct cttttgtttc cgttgctgtc atacctgaag gtggtttaat aatatcgaaa
     1441 aatacacctt ccatataaat tctaacagaa ggtgtacatg ctgtaaatat ttacctcata
     1501 tgtcttttgt tcaatgcctg acacatatag gatctttgca tctgacattt atctatttct
     1561 taccagtatg accaatcttc tgcaataact gtctatctgt cgtgctggca gctatattcg
     1621 atatattctg accttaatga aaaaagtgtg aacaagcagc ataaaaaaaa cgccaaccaa
     1681 attcggtggt tgacgttatc agcctcacaa ttattcgctt ttttcttctg cacagcagct
     1741 tttttcttcc ttcttgagct tgattttctt tactatctcg ggaaccatgc cgacaagctt
     1801 ttcaatgttt gacctgttct ttacgggaag cattgtgaca gaatcttgtt tgatgactat
     1861 tactgcatcc ggggatattt tggcgccaac tcccacacct gttcccattc cgtcgttgcc
     1921 cttttcatcc tttccgccgc cgcctccgcc gccgcagcca aaggccactg taataatcgg
     1981 gattagcgtt gtttctccta caactatcgg ctctccgatg actgtttctg ttttcaggaa
     2041 tttttccaga tggctgaata aagtttccag gctttcattt accggtgttt tactttccat
     2101 tattatatcc tcctataatt gttttcataa ttgtttacat tattatttac acttatatat
     2161 aaaaaaagtt tcaacttttt ttctttctga acaatatttt ccttacgggc tttttcaaag
     2221 cagttcttat cgtctgataa gccaggcttc ctactgtcat gctgccttca gcacgcaaat
     2281 ccaaatctat tatttcctgg gtaaagtcag gatataaccc tactcttgca tgaggaatca
     2341 tagactttac tactgccgca gcaccgcaca ccattcccgt aagcgaggga tcatcaaagc
     2401 cataggtcgc gtataaatgc atatacttcg gttttactat attcagcacc ttctttatat
     2461 attcaagaat ctcatttatc aatgtcctgt cggtaatctc cctgaatcga ggtacatttt
     2521 tttctttctc ttttttttct gttttctcct cggattgctc ctttttcccc tcctgcccct
     2581 tgcttttcag cttgcaaact ctcttatcca gtatccggag ggttacatct aaattttccc
     2641 cttccatttc agcgcttatc ccaaacagct ttcctgccca gccaaaggca aactgcgccc
     2701 tgaacccctc agcagtcaga acctgtcctc tgtaatttac aggtatgata agcagcagga
     2761 aaaccagccc caataggaaa agcagcaaat acagcaatat tttaacaatt atcaataata
     2821 caaccatcgg gtgtaccccc atttaataat tacctagcca agagcttctt gacttctttt
     2881 tttacaaggg attttataag attccccgtg tatgaagcag tccttttggc ccctttgcat
     2941 atgattttgc ttccgggaat gtatcctgcg aacttcaaag aattatccgc cactttcttt
     3001 ccgaacttga tactccgggt cagtgtatat ttaagcttct ccctcttatt cagcccatac
     3061 atgcagtcat caacaagcct cgcaatattc atatctgggt ttcttatttc cgaatcatca
     3121 tcaaagaact tatacagctc attctccaga gtcatgacca tatcaagttc cttcctgcac
     3181 tcaccgcagt agttaatatg ctctttaaga aaaataaatt ccagcgggtc aatggtgtta
     3241 tcggcatatc tctgtatgat ttccctgtca aatccacagt tcatttccgc acctccaagc
     3301 ttcatttcat tgcattcctt agtttttcct tcaaaatgtt tctcgccctg taaatatccg
     3361 ttttgacagt gccgagaggc tccttcagca ctgctgcaat ctcctcgtag cttaatcctt
     3421 cataatatct gaggatcaat accatcctgt gttttggcct aaggcactcc atgttttctc
     3481 ttataatatg ccccaattca ttatccaaga cttcggattc cacatccttc gaagcggcaa
     3541 ctacctcttc aattgttctg tcatcttcat accctgcatt aagcgatacc gaatcatatc
     3601 tgatagtcct tttgaagttg aggcatgtat ttaccgcaat tgttctgaac catgggtgaa
     3661 agggcatttt gacatcaaaa cttgatatat tgttaaaaac ctttatgtaa acctcctggg
     3721 caatatccaa agcatcctgc tcattctgca cataattata gcaaagctta tacagatatt
     3781 tttcatacat tttgaaaagc tctgtgaagg cagacttgtc atacttcttg cacctaacaa
     3841 taatgccggc ttctacattc acagcaccac ctccaggcca tacagtatat tacacaaaaa
     3901 aaccataaat tgtttcattt tttttctgtt tatttaactg ctaaaaatat ataaaaaggt
     3961 aaagctatat atatcgtgaa aaagattcat cattacaaaa tcagtagata tgtctaattc
     4021 tgtattaaag taatgtactt ttcacgatat atgcatcaag gaaaatgttt cgcactattt
     4081 aattcagcat aagatgttaa ctgcgaaaca tctaaataac tcattatgaa aggatgatgc
     4141 ttgtgaaggt taaacaaatt atgaccaccg atgtcagcac tgttacgcca aatgatacag
     4201 ttaccaaagc cgcatcaatt atgaagcagc taaatgtagg ctcggtacct gtaactgacg
     4261 gcagcagagt agtcggtatt gtaactgaca gagatattac acttcgcggg gtcgcaaaaa
     4321 gcggagaccc aaaccaaaaa atttcagaag taatgaccac tgatgtcaaa tatgccaccc
     4381 cggacatgga tgttcacgct gttgccgatt taatggcaga aaaccaggta agaaggcttc
     4441 cggtcataga taatgatagg cttgtcggta tcgtagcaat cggagacctg gcagtagaaa
     4501 acatatttga gaacgaagcc ggtgaagcac ttcataacat atcaatgggt gtaaggcatt
     4561 aataattttc tttaatctcc ggccttccgg agatttcttt ctctctgata tcctgcatca
     4621 gctgattgct gcatgcagct cttttgtaat aagttgctag tataatatta ccacttattt
     4681 ctttaatatt aaaccttctt atatattaat atttctcnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnntat caacctttat acctgagagg tgtgcctgtt gcttatagtt
     4861 tttgtcagaa cgctgattct atatatattg ataattgtag tattacggct gatgggaaaa
     4921 aggcaggtag ggcagcttca gccttcagag ctggtagtag ctttgataat tgcagacttg
     4981 gcggcagttc ccatgtctga agtgggtatt cctttgatta acggaataat acctataatc
     5041 actcttttca tcatggaaga gcttttatcc tacatatcca tgaaaagtga aagagcgagg
     5101 ggcctgataa gcggcaagcc cagcatactt attgagcggg gaacaatcat cgaagatgag
     5161 ctaagacgta ttagatataa catcaatgat cttttggagc aattaaggtt aaaaaacttc
     5221 tccaatgttg aagatgtcag ctatgccata ttggaaacca gcgggcagct aagcgtaatt
     5281 ccgaaggagg aaaaaaaacc tgtcacactg aaggacatga atttaatagc caaaaccggg
     5341 cacctgcctg tcaccgttat aattgacggg cggataatct ccgataacct gtacaaaatc
     5401 ggcctgagca ataattggct tgcagatcag ctcaggaaaa acagtattaa atcatctgaa
     5461 gatgtgttct ttgcatatct aaacccggag aggaagttta tataccagct ccgtagtaat
     5521 tcgcagaaaa aatgattgca gaaaggggcg gcggacgaat tgaaaccctt gataataatt
     5581 atttcactta ctattctgat tgttgccgga ggctcaataa cactgtattt attgaattcc
     5641 gaatcccaaa ggcttgacga cagtctttcg tcccttgggg aagccattga gaatgggaat
     5701 tgggataatg cttcaaaaaa cctcaaagaa tttaacagca aatgggacag gacaagttca
     5761 ttatggtcaa tgcttgtgga ccactatgaa attgataata tagaactgct tctctcacag
     5821 cttgcttcct atgtgaaaaa caagaataga aatgatgccc tgtcaagtgt ttcctcttta
     5881 aaaaccctta tcaaacacat acctgaaaag gagtccttaa gccttaaaaa tgttttttaa
     5941 taagttcttc catatccgaa gggacctggg ctgtaaaatc cagcatcctg ttttctatgg
     6001 gatgaagcat ttttatttta taggcatgca gtgcgtgcct ttttattttt tccagtcttc
     6061 ctccgtagag ttcatcccca aggagatgat gtcccagatg agccatatgt actcttatct
     6121 ggtgtgtccg tcccgtctcc ggtacaattt taaggaggct gcagtcctca tgcctttcaa
     6181 ctacgctgta atgtgtaact gcttcggctc cgtcctctct cacaaccctc cgtatcgaat
     6241 gaagcttttc ccttgctata ggtgcgtcta ttgtgcctga atctgcctca accacgccct
     6301 gtacaacagc cagatactct tttgttatat tgctcctgta accttctgcg gacatgagat
     6361 gctggatatg agaactcttt gcgaaaataa caaggccgga ggtattttta tcaagcctgt
     6421 taaccggcct tatggttata tccctgctct cttccatcca gtggtacatg aggccgtttg
     6481 ccagggtgcc catataatga ttccgggagg ggtgggtggg tatgcctgct tccttgttta
     6541 tcacaattaa ataatcgtct tcaaatatta tatccagggg catgaattca ggcaggatgt
     6601 tgtcggagtt ccggtcagga agttcaaatt ccaccttgtc tccggctttt ataatgatgt
     6661 ctgtgtgtgc tttgaatccg ttcaccttca gggaaccgga ctttttcacc tttatcagtc
     6721 cgttccttga catcataagc ctgcttctaa ggtaaacccc cagtgtaatg ccgtcattct
     6781 ccttgtcgat tatatatgta ttatagcttt tcaacttctt ctcactccaa ttcaagcctt
     6841 gatgcaagca accggatatc ctccgggaat tctgcatgaa gcttgagcaa attgcccgtt
     6901 acaggatggg ccagctccat gtccgatgca tgcaatgcct gcctgccgat aagacagctt
     6961 ttttttccat acagagtgtc tcctacaata ggatgcccca gaaagctgag atgtaccctt
     7021 atttggtgag ttctgcccgt tacaagctcc aggcgcacca gggaatggct cttgcctgtt
     7081 tttaacgtat catacttggt aactgccggc tgcccgtcac tttttacctt tcgctttatg
     7141 ggatccgatt cgtcctttcc gatgggtctg tcaatgaccc ctttttcagg tgcggctatt
     7201 ccctcaataa ctgctgtgta gaatttcctt actccccctg acctcatcat ttcatcaagc
     7261 ctgccgtgta ccagcccgtt ctttggcatc acaacaattc cggaggtatt catatccagc
     7321 ctgttcagaa ggcgcagcga tacttcctcc cccttccctg caaaataata agcggctgca
     7381 ttaagcaggg ttcccccggg gtggttgggt gtagggtgca ccagcatata aggaggcttg
     7441 ttgactgcca agaggtattc gtcttcataa ataatatcaa gcttaatgtt ttccggaact
     7501 atatcctctc tcgcctctac ggacaaatcc acctcaataa tatctccatc cttcagcttg
     7561 ttatccctgt atgccgtttt tccgttaata catacctttt tgtccttgaa cagcttcttt
     7621 atctgcctgg aggaaaaacc aagctgctgc gacatataag cgtcaacaaa tattctattt
     7681 tcactgagcc ttaccgggta ttttttttgc atgccatcgc cccgctacgc tttattattt
     7741 acttatgttc ctaataatta tagcatatta tacaaataat gggacgtgag tgggacacgg
     7801 ggacgtttct cctgtccc
//
LOCUS       DAOQUR010000084         7457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_10917_length_7457_cov_10.913402, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000084 DAOQUR010000000
VERSION     DAOQUR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10917_length_7457_cov_10.913402"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PKI50_11520"
     CDS             complement(<1..1046)
                     /locus_tag="PKI50_11520"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNT04369.1"
                     /translation="MSEKLNVAVVFGGKSGEHEVSLMSATNVINAMDKDKYNIFMIGI
                     TRQGKWMAYRGDISKIIDSSWEKEAEELDREKTVDIMFHRFFKDSTGEKIDVVFPVLH
                     GPNGEDGTIQGLFEMLDIPYVGCGVTASALGMDKAFSKQLFKAAGLNVGEYTVILKRD
                     IEKDIDSVVKLAEDKFKYPVFIKPANMGSSVGITKAHSRDELEAGLKEACKHDRKIII
                     EENINCREVECSVLGNNDPVASVVGEILPCHEFYDYEAKYFDDGKSGLIIPAELSESK
                     TQEIREAAIKAYKALDCCGMARADFLVDKETEKVYINELNTIPGFTKISMYPKLWDAA
                     GLSYGKLIDRLIELA"
     assembly_gap    1150..1249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1407..1580)
                     /locus_tag="PKI50_11525"
     CDS             complement(1407..1580)
                     /locus_tag="PKI50_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HNT04370.1"
                     /translation="MSRNRGMMSENLKYEIARELGVDRIVSSEGWGGVSSRDCGNIVK
                     KAIEIAERSLSGK"
     gene            complement(1706..3088)
                     /gene="ypeB"
                     /locus_tag="PKI50_11530"
     CDS             complement(1706..3088)
                     /gene="ypeB"
                     /locus_tag="PKI50_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HNT04371.1"
                     /translation="MKLFKMQLDRKQYHKVAVVLAAALILAGFFGYNTYTEKKQYQTF
                     LQNSYQRSFRELVTNVENIKLLLEKAEVSASPQQSSALMSQTWMQSYSAAENLGQLPI
                     THVSLSKTEKYLNQVGDYSYSLSRTGARNKNISEKDMDQISKLRAYAGELLKELHNME
                     EDVARGKIRFGEIRKKGRLALRRASEDAVEVSFGNIEDKFSEYPSLIYDGPFSDNVIE
                     GKPKGLDGEDVSLEKAKEKARKFVGEDKAGRIIEVSSGKGKIHTYGLEVSPKDKEKGN
                     PINIDITKKGGYVLWMLNPRDIPEKKLTDQQASDKAQKFLKEQGFGDLTETYFLKNDN
                     TTTITFIGVSKDGVLIYPDLLKVKVALDNGEIVGFDAYQYLMSHRKRDIPKPGLTEEE
                     ARKKVSPRIKVERVKLAIIPMPGNKEQLCYEFKGKYNNFDYFVYINAEDGSEENILRI
                     IKDENGTLTQ"
     gene            complement(3331..3966)
                     /gene="sleB"
                     /locus_tag="PKI50_11535"
     CDS             complement(3331..3966)
                     /gene="sleB"
                     /locus_tag="PKI50_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HNT04372.1"
                     /translation="MYSSYLTSQISSEAGAILLKWGSRGSEVREVQTRLKNWGFYKGS
                     VDGIYGWRTANAVKAFQRKHGLKADGIVGNATGKALGIRTGTASARGGTSRSNETYTL
                     AQCVHGEARGESYIGQVAVAAVILNRVDDPRFPKTIAGVIYQPGAFTAVTDGQMFQPP
                     GDTALKAARDALNGWDPSGGALYYYNPAKTTNKWIWSRPIIKVIGKHYFCK"
     gene            complement(4099..4770)
                     /gene="spoIIR"
                     /locus_tag="PKI50_11540"
     CDS             complement(4099..4770)
                     /gene="spoIIR"
                     /locus_tag="PKI50_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HNT04373.1"
                     /translation="MKYKIVFFPIAFFFVFGILTGIYINTDMALADVPEKIIRLHVVA
                     NSDSPVDQQLKLQVRDKIISSMSGRLEGLKDISEVKSIVRGSLAEIEAAAREVIEENR
                     KLYGVKAAFTEMDFPTRAYGNITLPAGTYQALNIVIGEGKGKNWWCVMFPPLCFIDVA
                     HGVIAEETMKELRTSLTEEEYSLLISSKEPGEVSVKLRFKILELAKSMGLRLAKLGNL
                     QNISK"
     gene            complement(5034..6173)
                     /locus_tag="PKI50_11545"
     CDS             complement(5034..6173)
                     /locus_tag="PKI50_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HNT04374.1"
                     /translation="MRAIKSLKAAAVLLCVLLLAGCWDKIEIEDRLFVLSIGVDSAEA
                     AEQQVSEDKYTLSFVSPVVDQIKEGPGPAFHTYKTVDNSIIMSLSQLLERFSKKQFFG
                     HTRNIFFGEDILKDEKLLKEILDGISRFHEFHNSMYAYFVPGRAEEVFKVKPMYDKLL
                     APYIAGIIENSDYTSKIIKLTLSEMIIMLENQEGGLVIPRINPGKEEVKINGAGVLKN
                     YKLIGYLGDQETSVYNWLTNKVRGGDIAIEHNNISTVFRHFTFDRKIELSKVENGNIY
                     LNYKMETEGSIEEYMIGKEIMDNALLKEISREVEKRIEDESEKLIKKFQQEFKTDLIG
                     ARDYLSKYQPKLYKTIEKDYEKFFTDSMVIEVTANVNIRRVGLIK"
     gene            complement(6160..7269)
                     /locus_tag="PKI50_11550"
     CDS             complement(6160..7269)
                     /locus_tag="PKI50_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HNT04375.1"
                     /translation="MIRNNDTISPYQLAMITIMTIISVEVFSLAAEAADELGTDGWLV
                     IVILGIINIFAALVIIRLNSIFPGKTIAEYSQIIIGKIPGKVLTGLFAVYLIIVIAYV
                     TRVFTEVVKMFLLFRTPTEVIMLSLILVCTYAVRGGIECVARVNELIFPILFIPFFIV
                     LVFGFPIMDFSNLRPILQTPPDKILASIPRMIFSFGGIELALYYIGFMKEPGKAYKPL
                     IISVIFILLFFVMITVFCLAAFGAKAVTRIIWPLAAYIRTVSLPGVFLERLDGVILSL
                     WVLTVFTTIVSVLFIVSYSISKAAGTKEQKQFVLPLVIVIYYLALQPESLAELYEWGS
                     LIFPYAVAAFMYVVPAALLLTAKLRKMEVKRNESN"
     gene            complement(7256..>7457)
                     /locus_tag="PKI50_11555"
     CDS             complement(7256..>7457)
                     /locus_tag="PKI50_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HNT04376.1"
                     /translation="KLKSFGIPYMSPYVSFVGSDFKDTIIRVPAFLMAKRPVLLARDK
                     IRLKNNRNLINRLEQEGSDDKK"
BASE COUNT         1788 a         1903 c         1349 g         2317 t
ORIGIN      
        1 gctaattcta tcagccggtc gataagttta ccgtagctca atcccgctgc atcccaaagc
       61 ttgggataca tacttatttt tgtaaagccg ggaattgtat taagttcatt tatatatacc
      121 ttttccgttt ccttgtcaac caggaagtcc gctctggcca ttccgcagca gtccagggct
      181 ttgtatgctt tgatggcggc ctcccttatc tcttgggtct tgctttctga aagctctgcg
      241 ggaattatca gcccggattt gccgtcatca aaatactttg cctcataatc gtaaaactca
      301 tggcagggga gtatctcacc gacaacagag gcaacggggt cgttattccc aagcacggag
      361 cattcaacct ccctgcagtt gatgttctct tcgattatta tctttctgtc atgcttgcac
      421 gcctctttca gccctgcttc aagctcatcc ctgctgtgag ccttggtgat gccgacactg
      481 gagcccatat ttgcaggctt gataaataca gggtatttga atttgtcttc cgccaatttg
      541 acaaccgaat caatatcctt ctcaatatcc ctcttcagta ttacggtgta ttcacctacg
      601 ttgagccctg cggctttgaa caactgtttt gaaaatgcct tatccatacc taaggctgat
      661 gctgtgaccc cacagcctac atagggaata tcaagcattt caaaaagtcc ctgtattgtt
      721 ccgtcctctc cgttaggccc gtgcagcaca ggaaatacta cgtcgatttt ttccccggta
      781 ctatccttga aaaaccgatg aaacatgatg tccacagttt tttctctgtc cagctcttcc
      841 gcttctttct cccagctgct gtcgattatt ttgcttatgt ctccccggta cgccatccat
      901 ttgccctgtc ttgttattcc tatcatgaat atattgtatt tatccttatc catagcattt
      961 atcacattag ttgcagacat aagagatact tcgtgttctc ctgatttccc gccgaataca
     1021 actgctacat tcagcttctc actcataaaa tttcctccaa aaataagatt ctattccggc
     1081 aacctctact acttattatt attctactct catggatatt attaatcaat aaaagattag
     1141 tcacggggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gggacgtttt
     1261 gggacatggg gacgtttcac gtgtcccctg agggacaggc gaaacgtccc catgtccctg
     1321 cgtccccgtg tctccctccc tgtcgcatta ttcttcgaat agtaaaaccg ggctgaacca
     1381 ataaatggcc cggcccggtt ttccgtctat ttgccgctta agcttctctc agcaatttca
     1441 atagcctttt tcacgatgtt tccgcaatct cttgaggata ctcctcccca gccttcagag
     1501 cttactattc tgtcgacgcc gagttctcta gctatttcgt atttcaggtt ttcagacatc
     1561 attcctctat tcctgctcat agccgatcct ccttatacaa cataaagctg cgtctctcgc
     1621 ggcattgtca ttccaactct gctgcatata tattttctgt gtatttgatt gattttatgt
     1681 gatgaaaaat gtggccctcc aagtattact gtgtcagtgt cccattttca tcctttatta
     1741 ttctgagtat gttctcttcg cttccatcct ctgcatttat atacacaaaa tagtcaaaat
     1801 tgttatactt ccctttgaat tcataacaca gctgctcctt gttgccgggc atagggatta
     1861 tcgccagctt taccctttcc accttgattc tcgggcttac tttctttctt gcctcttctt
     1921 cagtcagccc gggcttcggg atgtccctct tcctatggga catcaggtac tgataagcgt
     1981 caaatcctac aatctctcca ttgtccaggg caaccttgac cttcagcagg tcggggtata
     2041 tcagcactcc gtcttttgaa actccgataa aggttattgt tgtcgtattg tcattcttca
     2101 ggaaatatgt ttcggtaaga tccccaaagc cctgctcctt taggaacttc tgtgctttat
     2161 cactggcctg ctgatccgtc agctttttct cgggtatatc cctggggtta agcatccaaa
     2221 gcacatatcc gcctttcttg gttatatcta tattgatcgg attgcccttc tccttgtcct
     2281 ttggagatac ctccaggcca taggtatgta tcttgccttt ccccgagctg acctcaatta
     2341 tcctgcctgc cttatcctct cctacaaact tccttgcctt ttccttggct ttttcaaggc
     2401 ttacgtcttc tccatccagt cctttcggtt ttccctctat gacattgtct gaaaagggcc
     2461 cgtcataaat cagtgacggg tattcgctga acttatcctc tatgttgccg aagctgactt
     2521 ccaccgcatc ctcggatgcc cttctaagtg caagcctgcc cttcttgcgg atttcgccaa
     2581 acctgatctt tccccgggcg acatcctcct ccatattgtg aagctctttc aaaagttctc
     2641 ccgcataagc cctgagcttt gatatctgat ccatatcctt ttcacttatg tttttgttcc
     2701 gggcgccggt tctggaaagg ctgtagctgt aatcacccac ctgattcaga tatttctccg
     2761 tcttgcttaa tgaaacatgg gttatgggca gctggcccag attctccgcc gcggaatatg
     2821 actgcatcca ggtttgggac atcagggcac tgctctgctg cggagaagcc gatacctccg
     2881 ctttttcaag cagcagcttg atgttctcca catttgtcac caactctctg aaggacctct
     2941 ggtagctgtt ctgcaaaaaa gtctggtatt gcttcttttc cgtataggtg ttgtatccga
     3001 agaaacccgc aagtatcaaa gccgcagcaa gaaccaccgc caccttgtgg tattgttttc
     3061 tgtccaactg cattttaaat aatttcataa tatccctcct tcatgtatgt gcagggtttg
     3121 taaaatagtc cgactgtagg ggaagtatgt cctcctcacc ttccaaagcg ggggatgcaa
     3181 gggagagaag ccccctgccg ataaaggagg gtgctcagat gcttgcatcc gtcgaaggga
     3241 accatagggt tccctagcaa aggaaaagcg tccccgtaac ctcgcaacct cgtgccccgt
     3301 gcctgaccat aaactccgtc ttcttcggca ttacttgcag aaatagtgct tcccgatcac
     3361 cttgatgata ggcctcgacc aaatccattt attggttgtt tttgccggat tgtagtaata
     3421 cagcgctcct cccgaaggat cccatccgtt cagtgcatct ctcgccgcct tcagggcggt
     3481 gtctcccgga ggctgaaaca tctggccgtc ggttacggcg gtgaatgccc ccggctggta
     3541 aattactccc gcaattgtct ttggaaacct cgggtcgtcc actctgttca aaataactgc
     3601 cgctactgcc acctgcccta tgtacgattc tcccctggcc tcaccgtgca cacactgggc
     3661 aagggtataa gtttcattgc ttctggatgt gcctcccctg gctgatgcag ttccggttct
     3721 tatacccagg gcttttccgg ttgcattgcc tactataccg tctgccttaa gtccgtgttt
     3781 tctttgaaat gcttttacgg catttgctgt tctccagccg tatattccat caacgcttcc
     3841 tttgtaaaaa ccccaatttt tcagccttgt ctgtacctct ctcacttcac tgcctctgga
     3901 gccccatttc aataatatgg ctcctgcttc actgctgatt tgggaagtaa gatagctgct
     3961 gtacacccag aaaccaaagc ctgcatatat gattactata actataaaag gaatcagcct
     4021 tcctggtctt tttttgaaca ctaaaaacac ctcctgccct tattttttgt aacaggaggt
     4081 gttttattca tattttaatt atttgcttat attctggagg ttccccagct ttgccagcct
     4141 cagccccatg cttttcgcca gttcaagtat tttgaatctg agcttaacgg atacttcccc
     4201 cggctctttg gaggaaatca gcaggctata ttcctcctct gtcaatgatg tcctaagttc
     4261 tttcatggtt tcttccgcta ttacaccatg ggcgacatca atgaagcaca gaggagggaa
     4321 catgacacac caccagttct ttcctttccc ttctccgatt actatattca aagcctgata
     4381 tgtgcctgcc ggaagtgtaa tgtttccata ggcccgggtg ggaaaatcca tctctgtgaa
     4441 ggcggctttt acaccataga gcttcctgtt ctcttctatg acttctcttg cagcagcctc
     4501 tatttcggcc aggcttcccc gtacaatgct ctttacctct gaaatatcct tcagcccttc
     4561 tagcctccca ctcatactgc ttattatctt gtcccttacc tgaagcttaa gctgctgatc
     4621 aacaggcgag tcactgtttg ctaccacatg gagccttatt attttttcag gcacgtcggc
     4681 caatgccatg tctgtattta tgtaaatgcc tgtaagaatg ccgaaaacaa agaaaaatgc
     4741 aatcggaaaa aaaacgattt tgtatttcat actcacccct ccaatttata ttaactaaaa
     4801 cacgataacg caggtaacac taaacacacg gatttcccac ggaatacacg gcgataatat
     4861 tatacttttc cgtatcgctg cattagtaaa tatcgatacc ccctaaatta tcaattaccc
     4921 aataacatat ctgcgtcgtt ttttaattat ccgtgccttc agtgaatttt agcgtttttg
     4981 gtgttattcg ttcccctgtg cctgttattt ttgccatgtt ttgaagggtt tattcacttg
     5041 attaatccta ctctccttat attgacattg gcggtgactt caattaccat actatcggta
     5101 aaaaactttt catagtcctt ttctattgtt ttgtaaagct tcggctgata tttgctcagg
     5161 tagtcccttg ctccgataag atctgttttg aattcctgct gaaatttttt tatcagcttc
     5221 tcactttcgt cctctatcct tttttccact tccctgctga tttccttaag caaagcgtta
     5281 tccattattt cctttcctat catatactct tcaatactgc cttcagtttc cattttgtag
     5341 ttcagatata tattcccatt ttccaccttg gacagctcaa ttttcctgtc aaaggtaaag
     5401 tgcctgaaaa ccgtggaaat attattatgc tcaattgcta tgtcccctcc cctgaccttg
     5461 tttgtcagcc aattatatac tgatgtttcc tgatcgccca aatatccgat aagcttataa
     5521 ttcttgagaa ctcctgcgcc atttatcttt acctcttcct tccccggatt aatccttggt
     5581 atgacaaggc ctccttcctg gttttccagc attattatca tctctgagag agtaagctta
     5641 ataatctttg aagtatagtc actattttct attattcccg caatgtacgg tgcaagtaat
     5701 ttgtcataca taggcttgac cttaaatact tcctcagccc gccccggtac aaaatatgcg
     5761 tacatgctgt tatgaaactc atggaacctg gatatgccat ccaatatttc tttaagcagc
     5821 ttctcatctt tcaaaatatc ctcaccaaaa aatatattcc tggtatggcc gaaaaactgc
     5881 ttcttggaaa acctctctaa aagctgcgac aaggacataa taatgctgtt atccactgtt
     5941 ttgtatgtat gaaaggcagg ccccggccct tcttttatct gatctaccac cggggaaaca
     6001 aaggaaagag tatatttatc ctcagaaact tgctgctccg ctgcctctgc actatctact
     6061 ccaatagata atacaaataa cctgtcttct atctctattt tatcccagca gccggcaagc
     6121 agaagcacgc acagcagcac cgctgccgct ttcaagcttt taattgctct cattccgttt
     6181 cacctccatc ttccttagct tggctgtcaa aagtaatgca gcgggcacta catacataaa
     6241 ggcggcaaca gcatagggaa aaattaagct gccccattca taaagctcag ctaagctttc
     6301 gggctgcagt gcaagatagt atataactat taccaaaggc agcacaaatt gcttttgctc
     6361 ctttgtacct gccgccttag atatggaata gcttacaatg aaaagcaccg atacgatggt
     6421 agtgaatacc gtcagcaccc aaagggacag tattactccg tcaagccttt ctaaaaacac
     6481 tccgggaaga ctgactgtcc ttatgtaagc agccaaaggc catattattc ttgttaccgc
     6541 ctttgctccg aaagcagcta agcaaaagac tgtaatcatt acaaagaata ataatatgaa
     6601 aataacagag attattaaag gtttatatgc cttcccgggc tccttcataa aacctatata
     6661 ataaagggca agctctatgc cgccaaagct gaatatcatc cttggaattg aagccaatat
     6721 tttatccgga ggtgtttgca ggattggacg caaattgctg aaatccataa taggaaaccc
     6781 gaataccagt acaatgaaaa atggtatgaa gaggataggg aatatcagct catttaccct
     6841 tgcaacgcac tcgatacctc ctcttactgc atatgtgcaa accaaaataa ggctaagcat
     6901 gattacttcg gtaggagtcc tgaataacag gaacatcttt actacctcgg taaaaaccct
     6961 ggtgacatat gctatgacaa tgataaggta aacggcaaaa agacccgtca atacctttcc
     7021 cggaattttt cctataatta tctggctgta ttctgcaatg gtcttgccgg gaaatatgga
     7081 attaagcctg ataataacca atgcggcaaa aatgtttatt atccccaaga ttacaattac
     7141 cagccagccg tcggtcccca gctcatctgc agcttctgcc gcaagggaga atacctctac
     7201 agaaattata gtcataatag taatcatcgc aagctggtat ggagatatgg tgtcattatt
     7261 tcttatcatc actgccctcc tgttccagcc ggtttataag gttcctgttg ttcttcagcc
     7321 ttattttgtc cctggcaagc agtacaggcc tttttgccat aaggaatgca ggtaccctta
     7381 taattgtatc cttgaagtcg cttcccacaa aggatacata gggcgacata taaggtattc
     7441 caaagctctt aagcttt
//
LOCUS       DAOQUR010000085         7438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_10957_length_7438_cov_10.386699, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000085 DAOQUR010000000
VERSION     DAOQUR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_7438_cov_10.386699"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..888
                     /locus_tag="PKI50_11560"
     CDS             196..888
                     /locus_tag="PKI50_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNT04377.1"
                     /translation="MIGTGLGGILTIFLGNPTKRFMAVLLGFTSGIMISVVCFDLLPE
                     AFGMGGVSLSLLGIVLGVGMILVIEQFIPERVSQKFSSDFDFVRSGILLGIGIAVHNF
                     PEGLAVGSGFAASDYLGLGLAIVIGLHDMPEGVAMAAPLKMGGINRLKILFYTVLAGI
                     PMGFGAFVGELLGEISHTFICLCLSFAGGAMLYITCGELMPKTQNIYKGRISTIGMII
                     GIMCGIIISTNL"
     gene            975..2021
                     /locus_tag="PKI50_11565"
     CDS             975..2021
                     /locus_tag="PKI50_11565"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNT04378.1"
                     /translation="MDTEVFFLDENDIDFDIIDKAAEIIRNNGTVVFPTETVYGLGAN
                     ALSGEAVKKIFAAKGRPSDNPLIAHVSSMNMLLYLIGSHLKAGTGLLIDKYWPGPLTL
                     IFKKSGKVPDEVTAGMDTIAVRMPDNRIALELIERAGLPIAAPSANISGKPSPTTPEH
                     VIADMRGKVNMILCGGKSQVGVESTILDMTGDVPVVLRPGGLTLEELENVLGPVRVSG
                     GQVGDKEIPRAPGMKYTHYAPDAEMIIVKGELDNIKKKIQELAAEYSAGGMKVGVLAS
                     DETAGYYKDCRVLSLGSRSDVRVIASNIFEKLREFDKIGVDIIFAEAFDESHMGMAVM
                     NRMKKAAGFNIIEV"
     gene            2024..2494
                     /locus_tag="PKI50_11570"
     CDS             2024..2494
                     /locus_tag="PKI50_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HNT04379.1"
                     /translation="MKRILFVCTGNTCRSSMAEALFRHSLLESGLAGEYDASSAGTSA
                     FPGMPASHNAIQALEALGIDLSGHSSSLINNDIIDKADVILTMTASHKKSLLRFRPDI
                     AFKTYTLAEYIGAEDSGDIEDPFGGDLDIYMNCRDKINRYIAALINKLKEKGEY"
     gene            2496..2948
                     /gene="rpiB"
                     /locus_tag="PKI50_11575"
     CDS             2496..2948
                     /gene="rpiB"
                     /locus_tag="PKI50_11575"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNT04380.1"
                     /translation="MKIAIGSDHGGFLLKDEIMKHLKEKNIDFVDFGCFTTESVDYPD
                     IGQAVGEAVARGEYDKGIICCGTGIGISISANKIPGVRAALCGDCFSAKASREHNDAN
                     VLALGERVIGPGLAKMIVDTWLNSEFQGGRHGRRVEKIAKIEEKYRRN"
     gene            2977..3588
                     /gene="upp"
                     /locus_tag="PKI50_11580"
     CDS             2977..3588
                     /gene="upp"
                     /locus_tag="PKI50_11580"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNT04381.1"
                     /translation="MEHPLIQHKLTLLRDKNTGVKEFRELVEELALLMGYEVTRNLTL
                     EETEIDTPICRTKSRVIAGKKLGIVPILRAGLGMVDGMLKLIPAAKVGHIGLYRDPET
                     LMPVEYYCKLPSDVAERDLIVVDPMLATGGSASAAITFLKDRGAKSIKLVNLIAAPEG
                     IKAVVEAHPDVEIYVAAVDERLNDHGYIVPGLGDAGDRLFGTK"
     assembly_gap    3627..3726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3760..4248
                     /locus_tag="PKI50_11585"
     CDS             3760..4248
                     /locus_tag="PKI50_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HNT04382.1"
                     /translation="MDRRDKYNYYLDIAETVLERGTCLRRNYGAIIVKHDEIISTGYS
                     GAPRARENCCDLGYCIRQKLNIPRGQNYEKCRSVHAEANCIISASRRDMIGSTLYLVG
                     KDMESGEYVLDANSCSMCKRLILNAGIERVIIRDTADRYRAIEVSKWIEDDDSLSDEL
                     GY"
     gene            4548..5603
                     /locus_tag="PKI50_11590"
     CDS             4548..5603
                     /locus_tag="PKI50_11590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNT04383.1"
                     /translation="MTRSIIAFLIALLVSFAATPLMIRLAKHMGAIDVPKDGRRVHKV
                     PTPRLGGLAIFLGFLAGLLYLYEIDSRMVGVLTGAAIIVTLGFFDDIKPLPAKFKFLV
                     QIIAAVIVIRSGVRIDHVSNPLHFMFPDNEYIIFGNWSYPLTLLWIVGVTNAINLVDG
                     LDGLAAGISIISAVTLFVAAQTTGQPFAAFLASILAASILGFLPYNFNPAKIFMGDTG
                     ALFLGYMLSVISVMGVLKGAAALSILVPIFAIGLPIYDTLFAMIRRASNGKSMMEADK
                     GHLHHKLLDAGMSQRQAVITLYSISAVLGFSAVVLVEATLKVAFVLVFAVFLLASMGA
                     KYLSQVEVDQGSNKADL"
     assembly_gap    5606..5705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5715..6875
                     /gene="wecB"
                     /locus_tag="PKI50_11595"
     CDS             5715..6875
                     /gene="wecB"
                     /locus_tag="PKI50_11595"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNT04384.1"
                     /translation="MLIKVLSIFGTRPEAIKMAPLVNAMKADSRFESCVCVTAQHRDM
                     LDQVLEIFDIKPDFDLDIMKDRQSLTDITVNALLGLEKVIAGLQPDIILVHGDTTTTF
                     IGSLAAFYNKIRVGHVEAGLRTFNKYFPYPEEMNRKLTGAIADIHFAPTSVSKANLLR
                     EGVPDGSIFVTGNTAIDALKTTVKKDFRFYTEELKKLNYDKKRIIMVTAHRRENLGEP
                     LGNICRALKRIVQSHEDVEIVYAVHKNPAVRETVYGILGSIPEVHLLEPLDVDEMHNL
                     MGKCYMVLTDSGGLQEEAPSLGKPVLVLRRETERPEAIEFGTLKLAGVKEEDIFRLAD
                     TLLTDGKEYCRMANAVNPYGDGHASERIIAALLYYLGVEDKRPGDFVPNNRQ"
     gene            complement(7248..>7438)
                     /locus_tag="PKI50_11600"
     CDS             complement(7248..>7438)
                     /locus_tag="PKI50_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04385.1"
                     /translation="CLIDCPDFVHNSTSKVLIVKNEYFNIDLASNMPTKNAARAAFFS
                     LGNPMVPFDGCKHPERLL"
BASE COUNT         2022 a         1293 c         1991 g         1932 t
ORIGIN      
        1 gggaagggac acggggacgt ttctcctgtc ccgctgaaag cgggacagga gaaacgtccc
       61 cgtgtccctt cccgtcctca tgtctcattg ttctataatc ttcttttctg aatatacatt
      121 tatggaagat gtagaattgg aggctatagt gtgagcaggc tttttgctgt tacctgcata
      181 ggatttttat gcggtatgat aggtaccggc cttggcggta tattgacaat atttcttggg
      241 aaccctacaa aaaggttcat ggcagttctt ctcggattta ccagcggtat tatgatatct
      301 gttgtgtgct tcgacctgct tcctgaagcc ttcggaatgg ggggcgtatc cctcagcctt
      361 ttgggtatag tgctgggagt ggggatgatt ctggtaatag agcagttcat tccggaaagg
      421 gtaagccaga aattcagcag tgattttgac tttgtaagaa gcggaatact gcttggcata
      481 ggaattgccg tacataactt ccccgagggg cttgctgtgg gatccggttt tgctgccagt
      541 gactatctgg gcttgggcct tgctattgta ataggtctcc atgatatgcc ggagggcgtt
      601 gcaatggcag caccgcttaa aatgggaggt atcaacaggc ttaagatatt gttttataca
      661 gtgcttgcgg ggatacccat gggctttgga gcctttgtag gagaattgct gggagaaata
      721 tcacatactt tcatatgctt gtgcctgagc tttgcggggg gtgctatgct gtatataacc
      781 tgcggggaac tgatgccgaa aactcagaac atctacaaag gaaggatttc tacaataggc
      841 atgattatcg gcataatgtg cggaataatt atatcaacca acctctgaat atgaattcta
      901 aaagttgtat atgacaatct gctgtgctat tatgtatttg ggcataaaag cacatttttt
      961 ttggaggagc cgctttggat acggaagttt tttttcttga cgaaaacgat attgattttg
     1021 atataattga caaagctgct gagataataa ggaacaacgg cactgtggtt tttcccaccg
     1081 aaacggtata cggcctggga gccaatgccc tgtcggggga agcggtgaaa aagatttttg
     1141 ccgcaaaagg gaggcctagt gacaatcccc ttattgccca cgtttcctct atgaatatgc
     1201 ttctatatct catcggaagc catctgaagg ccggaaccgg gctgcttatt gataaatact
     1261 ggccggggcc cctgacattg atttttaaga agtccggcaa ggtacccgac gaggtgactg
     1321 cgggcatgga tactattgca gtaaggatgc ccgacaacag gatcgccttg gagcttattg
     1381 aaagagccgg actgcccatc gcagcaccca gtgcaaatat ttccggaaaa ccaagcccta
     1441 cgactcccga gcatgtgatt gcggatatga ggggcaaagt gaatatgata ttgtgcggag
     1501 gcaaaagcca ggtgggcgtg gaatcaacaa ttctggatat gaccggagat gtgcctgtgg
     1561 ttttgaggcc cggaggcctg actctggaag agcttgaaaa tgtactgggc cctgtaaggg
     1621 tcagcggggg acaggtaggg gacaaggaga ttccaagggc tccggggatg aaatacactc
     1681 actatgcccc ggatgcggaa atgattattg taaaagggga attagacaat ataaaaaaga
     1741 aaatacagga gctggcggcg gaatattcgg ccgggggcat gaaggtcgga gtactggcct
     1801 ctgatgagac tgcaggctat tataaggatt gcagggtgct ttcattggga agcagatcgg
     1861 atgtaagggt tattgcgtcg aatatttttg aaaagctcag ggaatttgat aaaatagggg
     1921 tagatataat atttgcggaa gcctttgatg aaagtcatat gggtatggcc gtaatgaaca
     1981 ggatgaaaaa agcggcaggc tttaatatta ttgaggtgta gatatgaaaa ggattttgtt
     2041 cgtttgcact ggcaatacat gcagaagcag catggctgaa gcacttttca ggcattcact
     2101 ccttgagtcg ggactggcag gggaatatga tgcaagctca gccggcacct cggcttttcc
     2161 cggtatgccg gcatcccata atgccataca ggctcttgaa gccttgggca ttgatttatc
     2221 cgggcattcc tccagcctta taaataatga tattattgac aaggcagacg tgatacttac
     2281 tatgactgcc tctcataaga agagtttgct caggttcagg ccggatattg cttttaagac
     2341 ttataccctg gcggaatata taggggcaga agacagcgga gatattgaag acccttttgg
     2401 cggggatttg gatatatata tgaattgccg cgacaaaatc aacagataca ttgcagcgtt
     2461 gataaataag cttaaagaga aaggggaata ctaatttgaa aattgccatc ggaagtgatc
     2521 atggaggttt tttgctgaag gacgagataa tgaagcattt gaaggaaaaa aatattgatt
     2581 ttgttgattt tggatgtttc accacagagt cggtagatta tcctgatatt ggacaggcag
     2641 tgggagaagc agttgcccgg ggcgagtatg acaagggcat aatctgctgc ggaacgggga
     2701 taggtatttc aatatccgca aacaagatac caggagtcag agccgcgctg tgcggagatt
     2761 gcttttctgc aaaagcatcc agagaacata acgatgccaa tgtacttgcc cttggagaaa
     2821 gggtaatagg gccgggattg gccaagatga tagttgatac atggctgaac tctgaattcc
     2881 agggtggaag gcacggcaga agagtcgaga aaatagcaaa gatagaggaa aaatatagga
     2941 ggaattaacg tggagcagta caagaatgtt acggttatgg aacatccgct tatacagcat
     3001 aagcttacgc tgctgaggga taagaacaca ggagtgaagg agttcaggga attggttgag
     3061 gaactggctt tgcttatggg ttatgaggta acaagaaatc ttacgcttga agaaacagag
     3121 attgatactc ctatatgcag gacaaaatcc agagttatcg caggcaaaaa gcttgggata
     3181 gtgccgatat taagggccgg cttgggaatg gtggacggta tgctgaagct gattccggca
     3241 gcaaaggtcg gtcatatagg actttacagg gatcccgaaa cattgatgcc tgttgagtat
     3301 tactgcaagc ttccttcaga tgtggcggaa agggacctta ttgtcgttga tccgatgttg
     3361 gctaccgggg gctctgcttc ggcagcaata acatttctta aagacagagg ggcaaagagc
     3421 ataaagcttg tgaatcttat agctgctccc gaaggcataa aagctgttgt ggaggctcat
     3481 cctgatgtgg agatatatgt ggctgcagtg gatgagaggc ttaatgacca cggatacata
     3541 gtgcccggac tgggggatgc gggagacagg cttttcggta caaaataaat aagtttgctg
     3601 atgcaattgt aaagaagttt gctgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnaagt ttgctgatag cagtaaaggg gaggaaggta tggatagaag ggacaaatac
     3781 aattactatc tggatattgc agagacggta ttggaacggg gaacatgcct taggagaaat
     3841 tacggagcta ttatagtaaa gcatgatgaa ataatatcca caggctacag cggagcgcct
     3901 agagccagag agaactgctg tgacctgggc tattgcatta ggcagaagct gaatattcca
     3961 agagggcaga attatgaaaa atgcaggagt gtgcatgctg aggccaattg cataatatcg
     4021 gcaagcaggc gggatatgat agggtctaca ttatacctgg tgggaaagga catggaatcc
     4081 ggtgaatatg ttttagacgc aaactcctgt tccatgtgca aaaggctgat attaaatgcc
     4141 ggtatagaga gagtgattat aagggatacc gcggacagat acagggcaat tgaagtaagc
     4201 aaatggatag aggatgatga ttcattaagc gatgaattgg gatactaagt atcagacacg
     4261 agatacgaga attagtgcag ggctttgaag ctgcgatata tggagagtta ttcagagcgc
     4321 agcgaagaat ccgatgtttt tacaatacag tttgctcctg tatgcatttt agttgttttg
     4381 tcattctgag cgcagcgaag aatctgatat ttttacatat caaatagttt tattttcagt
     4441 acataatgga gaaaataagt ttaacatttt gacattgaat ttgacctgat gtaaaatatt
     4501 agtacggtgt ttgtccatta gtaaactggt gatgttgggg tgattagatg acaagaagca
     4561 ttattgcgtt cttgatagct ttgttggttt cttttgcagc cacaccgctg atgataagac
     4621 tggctaagca catgggtgct atagatgtgc caaaagacgg caggagagta cataaggttc
     4681 cgacaccaag actgggaggg cttgcaatct ttcttggatt tctggcgggg ctgttatatc
     4741 tgtatgagat agattccaga atggttgggg tattgaccgg agcagcgata attgttactc
     4801 tgggattctt tgacgatatt aaacccctgc ctgcgaagtt caagtttctg gttcagatta
     4861 ttgcagctgt aattgttata cgcagcggag taagaattga ccatgtaagc aatccgctgc
     4921 atttcatgtt tccggataac gaatacataa ttttcgggaa ttggtcctat cctctgactt
     4981 tgctatggat agtcggagtt acaaatgcaa ttaacctggt agacggactg gatggcctgg
     5041 cagcagggat atcaataatt tcagcagtca cattgtttgt ggctgcacaa acaacggggc
     5101 agccttttgc ggcatttctg gcatcaatac tggcagcctc aatcttaggc ttcctgccct
     5161 ataacttcaa tccggcaaaa atattcatgg gagatacagg agcattgttt ttaggctata
     5221 tgctttcggt gatttccgtt atgggtgtcc tgaaaggagc tgccgcatta tcgattctgg
     5281 taccgatttt tgcaatcggg cttccaattt atgatacgct ttttgcaatg atcagaaggg
     5341 catccaacgg gaagtcgatg atggaggcgg ataaagggca tttgcatcac aagcttctgg
     5401 atgccggcat gagtcagaga caggcggtga tcacactgta cagcataagc gcggtgttgg
     5461 gcttcagtgc cgttgtactg gtggaggcta ctttgaaggt tgcgtttgtg ctggtgtttg
     5521 cggtttttct gttggcctct atgggggcaa aatacttaag ccaggtagag gtggatcagg
     5581 gaagcaataa ggcagatttg tagggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnggagc tgagattttg attaaagtac tgtcgatatt tgggacaagg cctgaggcga
     5761 taaagatggc accccttgta aatgctatga aagcggacag ccggttcgaa tcctgtgtct
     5821 gcgttactgc acagcacagg gatatgcttg accaggtact ggagatattt gatataaagc
     5881 cggattttga tctggatatt atgaaggata ggcagtcttt gaccgatatt accgtaaatg
     5941 ctctcctggg acttgaaaag gtgattgccg ggctgcagcc ggatatcata cttgtccacg
     6001 gggatacgac aacaaccttc atcggttcat tggcggcctt ttataataag attcgggtgg
     6061 gtcatgtgga agccggcttg agaaccttca ataaatactt tccatatccg gaggaaatga
     6121 acaggaagct tacaggagca atagccgata ttcatttcgc tcccacgtct gtttcaaagg
     6181 cgaaccttct gagggaggga gtgccggacg gaagtatttt tgtgaccgga aataccgcta
     6241 tagatgcttt gaagactacg gtgaaaaagg atttcaggtt ttataccgaa gaactgaaaa
     6301 agcttaatta tgataaaaag aggataataa tggtgacagc ccacaggcgt gagaacctgg
     6361 gagagcccct tggaaacatt tgcagggctt tgaagagaat tgtccaaagt catgaagatg
     6421 ttgagattgt atatgcggtt cataagaatc ccgccgtaag ggagactgtt tacggcatac
     6481 tgggaagcat tccggaagtg cacttgctgg agcctttgga cgttgatgaa atgcacaatc
     6541 ttatggggaa gtgctatatg gtgctgacag attcaggagg ccttcaggaa gaggcccctt
     6601 ccctcggcaa gccggtgctg gtgcttaggc gcgagactga aaggccggag gccatagagt
     6661 tcggaaccct taagcttgca ggggtgaagg aggaggatat cttcaggctt gcggatactt
     6721 tgctgacgga tgggaaggaa tattgcagaa tggccaatgc cgtcaatccc tatggcgacg
     6781 gccatgcatc agaaaggata atcgccgctc ttctttatta tttgggggtg gaggataaaa
     6841 ggccggggga tttcgtgccg aataatcggc aatagaaaat tcataaacat atagtatgcc
     6901 gaaatattgg ctatgagaag aatgcggcat aagttacggg taacacggga aacacggatg
     6961 tttcacggat tcctacggaa taacacggta ctattatata ttatatatct acatcaggat
     7021 aattcgatat ctgtatcatt ttcgataatt atctatgtat cggaatattt atcccgccgt
     7081 gcatttagcg ggttttagtg ttttcagtgt tatcagcggc ttcgaagata tcttcccata
     7141 aaatcgggat aatttataaa gtgcgtcgtg agacgcattt ttttattgaa taggaaagta
     7201 tactttccta ttcaatgggg gatgcaaggg ggagaagccc cctgccgtta aaggaggcgc
     7261 tcaggatgct tgcatccgtc gaagggaacc atagggttcc ctagcgaaaa aaacgcagcg
     7321 cgagccgcgt ttttcgttgg catattactt gctaagtcta tattgaagta ttcatttttc
     7381 actattaata ctttggaggt ggagttgtgg acaaaatcag gacaatcgat gaggcagc
//
LOCUS       DAOQUR010000086         7254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_11326_length_7254_cov_11.016252, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000086 DAOQUR010000000
VERSION     DAOQUR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11326_length_7254_cov_11.016252"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..1163
                     /locus_tag="PKI50_11605"
     CDS             447..1163
                     /locus_tag="PKI50_11605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT04386.1"
                     /translation="MGKKQRIDKILSNMGYGTRKEVKELISGGLVEIDGIVVKEPGQQ
                     VTADSQEIKVGGRKLLYKDFIYIMMNKPQGVISATVDSREKTVIDLLPDEFKAFKPFP
                     VGRLDKDTEGLLLLTNDGQMAHRLLSPKKHVPKTYIAKVAGRVSQEDAEAFGKGVILE
                     DGYKTMASKLQILVRGSISHVEVTIYEGKFHQVKRMFQAVGKEVIFLKRISMGELKLD
                     ETLEPGEYRELNEEELRILS"
     gene            1271..2299
                     /locus_tag="PKI50_11610"
     CDS             1271..2299
                     /locus_tag="PKI50_11610"
                     /inference="COORDINATES: protein motif:HMM:NF039924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNT04387.1"
                     /translation="MAMEIYGESDEPLVRIVNSPVVELICAVHLLTDAAHHQYELRWA
                     EEVLSGFKQEELEALNTIKLFSSGWMNMLNLILESGILTSIPEFIGYIERLDLLDFFY
                     FFLNEEVERTKIQKAFAYKKERSRIQEKTKWAVEESELMEFFEFPEKIKRDTINLFKK
                     VYAHDFMAKLDRCKEKMEAGIQYVESFTGKDSVESVLEKISGRSRKLFSGFKEYYIIP
                     SYFISPQKSLRIYSGDRIYVVFDCRVTRDKREALLEELSSVLKIIDDKSRMEILRILM
                     NNKSYGKALSDLVGLSTPTVSHHLDILKQAGLIKEEKIRNIKYFYADKDKLKKVVDDI
                     NKYFFGAE"
     gene            2440..3447
                     /locus_tag="PKI50_11615"
     CDS             2440..3447
                     /locus_tag="PKI50_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNT04388.1"
                     /translation="MKIREITENMEKNNLSKYASLSVNSKGRQTWEEKCDIRTDYQRD
                     RDRILHSKAFRRLKHKTQVFISPEGDHYRTRLTHTLEVSQIARTIARGLRLNEDLTEA
                     IALGHDLGHTPFGHAGEKVLNEIHPVGFKHNEQSLRVVDILEGGKGLNLTYEVRDGIL
                     RHTGDIGPETLEGNIVSFSDRIAYINHDIDDALRGGILEMSSLPEKCIAILGDTHKKR
                     INTMITDIITQSMDKNEIMMSSMVRQATDELREFMFENVYIGSEAKAEEKKTRNIISC
                     LYEYYVQNPEEMPIENIRENRDADNIDIERLACDYIAGMSDRFAINKFTELFIPSPWR
                     V"
     gene            3553..4005
                     /locus_tag="PKI50_11620"
     CDS             3553..4005
                     /locus_tag="PKI50_11620"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HNT04389.1"
                     /translation="MRILIDGDACPVTHLIEKTAARCGVGVVFFHSTCHYSNYSNAAA
                     EKIMVDNESQAVDMAIINKAVKGDIVVTGDFGLASLTLGKGAKAISFSGMIFDESNID
                     RLLFERHVSGVIRRSGGRTKGPARRTDSDDRNFEKSLSFLISQIMKDI"
     gene            4085..5854
                     /gene="dnaG"
                     /locus_tag="PKI50_11625"
     CDS             4085..5854
                     /gene="dnaG"
                     /locus_tag="PKI50_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNT04390.1"
                     /translation="MFFSEDKISEIIEKNDIVDVISEYMTLKRSGSNYMALCPFHSEK
                     TPSFSVSPSKQIFNCFGCGFGGNVITFIQKIERIEFVEAVKMLAEKAGVKLEEDKDPK
                     QLERAKQKKALYRINAEAARFFYSNLNAGSKALEYLRRRGLSDAIIKRFGLGYAPDEW
                     YGLMNHMKRKDIDTELLFKAGLIVRNNDGEKFRDKFRNRIMFPIIDLKGNVIAFGGRV
                     IDDSKPKYLNSPDTLVFNKGYNIYGLNFVKKVQNLENIVVVEGYMDAIALHQFGVENA
                     VASLGTAFTENQAKLLKRFSNEIIIAYDSDLAGQAATLKGLAILEKEGCVVKVLSLPS
                     GKDPDEYIRKEGIDAFRDRIRKSVSLIEYQIENLKRGLDINNIQDRIKFTKGFSEILK
                     NVVSNVEVDAYIRKYSGIMKVNEEALYAELNRLRKKNKNGNNKHNILNSIKTEPELKK
                     GEITAEIYLLNICIYYFKKVKNIFEILKPADFSNDIHVRIAEIIQRAYKEDKAITAGE
                     IISYFDNDDEKSRVAGIFSQKLPDSDVDGLIKSSLDKIRQSKLDRTIQEMTEKMNEMY
                     HAGKKEEANKIFVEIKELQKKKR"
     gene            5898..6965
                     /gene="rpoD"
                     /locus_tag="PKI50_11630"
     CDS             5898..6965
                     /gene="rpoD"
                     /locus_tag="PKI50_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNT04391.1"
                     /translation="MTYVKELVDKGKKNGSLTYKEIMDTFEEVEMDPEQIDKLYEYLE
                     TKGIDVVGAIDNDMDIPPLDEDVEEVDLDISIPEGISIDDPVRMYLKEIGKVPLLSPE
                     EEIDLANRIKKNDSEAKKKLTEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVE
                     KFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQQLG
                     RDPIPEEIAAEMGMSEDKVREIMKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAE
                     SAAFTLLKEQLMEVLDTLTPREEKVLKLRFGLEDGRARTLEEVGKEFNVTRERIRQIE
                     AKALRKLRHPSRSKKLKDFLE"
     gene            7028..7103
                     /locus_tag="PKI50_11635"
     tRNA            7028..7103
                     /locus_tag="PKI50_11635"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7061..7063,aa:Asn,seq:gtt)
     gene            7109..7185
                     /locus_tag="PKI50_11640"
     tRNA            7109..7185
                     /locus_tag="PKI50_11640"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7143..7145,aa:Ile,seq:cat)
BASE COUNT         2413 a         1189 c         1826 g         1826 t
ORIGIN      
        1 ggtataataa tgcgaaaacg aaggaaattg cgacggggac atggggacgc ggggacacgg
       61 ggacgtttcg cgtgtcccac tgaaagcggg acaggggaaa cgtccccgcg tccccgtatg
      121 agacacgggg gacgtttctc ctgtctcact gaaagcgaga cacgcgaaac gtccccatgt
      181 ctcgttatgc atcttacaga gtattgcctc gtatataaag gaaatgcaaa taccgacgtg
      241 gggaagctat attcgacgaa accttccacc tgacctcgga acggaaaggc ttccaggcaa
      301 aataaccaga cgggcttgca gagatgggac ccacgccagt gattttgctg gctttggaag
      361 gtgaggagaa catacttccc ctacagtcgg agtactttac aaacccttga ttataagtcg
      421 cattcttcct ataaggagat acagtaatgg gcaaaaagca gagaattgac aagatactat
      481 ccaatatggg gtatggcacc agaaaagaag taaaagaact gatttcgggc gggcttgtcg
      541 aaatagacgg gattgttgtg aaggagccgg ggcagcaggt gacggctgat tctcaggaaa
      601 taaaggtagg gggccgtaag ctcttgtaca aggatttcat atatataatg atgaacaagc
      661 cccaaggagt catatccgcc acggtcgaca gcagggagaa gacggttatt gacttgctgc
      721 cggacgaatt caaggccttt aagccttttc ccgtgggaag gcttgacaaa gataccgaag
      781 gcctgctttt gcttaccaat gacggacaga tggcacacag gctgctgtct ccgaagaagc
      841 atgttccgaa gacatacata gcaaaggttg cagggagggt aagccaggag gatgcggaag
      901 ctttcggcaa gggggtaatc ctggaggacg ggtacaaaac catggcatca aagcttcaaa
      961 tacttgtaag aggaagcatc tctcatgttg aggttactat atatgagggc aagttccacc
     1021 aagtcaaaag gatgtttcag gcagtcggca aggaagtgat atttttgaag cgcatttcca
     1081 tgggggagct gaagctggat gagactcttg aacccggaga atacagggag ctgaatgaag
     1141 aggaactgag aatactgtcc tgatttattt aaacttgagg ttgcatgttt gttagattat
     1201 tgataatata attaattaga tatatatcga attacttata taataagctt tttttaaagg
     1261 ggtgctgcaa atggcaatgg aaatatacgg agagtccgac gagcctttag taaggattgt
     1321 gaattctccg gttgtcgaac ttatatgcgc tgttcacctg cttaccgacg ctgctcatca
     1381 tcagtatgag ttaaggtggg cggaggaggt tctttccgga ttcaagcagg aagagctgga
     1441 ggctttgaat accattaagc tgttttcatc aggctggatg aatatgttga atctaatact
     1501 ggaaagcggc atacttacat caataccgga attcataggg tacattgaac ggctcgattt
     1561 gctggacttt ttttatttct tcttaaatga ggaagttgag agaaccaaga ttcagaaggc
     1621 atttgcatat aagaaggagc ggagcaggat acaggaaaaa acaaagtggg cagtagaaga
     1681 aagcgagctt atggaatttt ttgaatttcc tgagaagata aaaagagata ctataaattt
     1741 atttaaaaaa gtttatgctc atgattttat ggcaaagctg gaccggtgca aggaaaaaat
     1801 ggaagccggg atacagtatg tagaaagctt tacagggaaa gacagcgtgg aatccgtgct
     1861 tgaaaaaatc tccggaagat caaggaaatt gttcagcggg tttaaagaat actatataat
     1921 tccttcatat ttcatatcac cccagaaaag cctgagaata tactccggtg ataggatata
     1981 tgtggttttt gactgcagag tgaccaggga caagcgtgag gctttattgg aagagctgtc
     2041 ttcagtatta aagataattg atgataagtc gaggatggag atattacgga tacttatgaa
     2101 caataaaagc tacgggaagg ctctttccga cctggtgggc ctgtcaactc ccactgtatc
     2161 ccatcatttg gacattttaa agcaggccgg attaatcaag gaagaaaaaa ttagaaatat
     2221 caaatatttc tatgcggaca aggataaact aaaaaaagta gtggatgata taaacaagta
     2281 tttctttggg gcagagtagg gaaatcggct gtgcgaattt gcacggccga ttatttattg
     2341 taaaattttt tccgtgaaaa aggagatttc aagtttttgt tgtatattaa attaatgtgc
     2401 tatgcaattt tttgagatat tgcagaggga gtcgtgttta tgaaaatcag agaaataacc
     2461 gaaaacatgg aaaaaaataa cctctccaag tacgcctctt taagtgtaaa ttccaaaggg
     2521 aggcagactt gggaggaaaa atgcgatata agaaccgatt atcagaggga tagggacagg
     2581 atactgcatt cgaaggcatt caggaggctt aagcacaaga cgcaggtatt catatctccc
     2641 gaaggggatc attacaggac aaggctgacc catactctgg aggtatccca gatagcgcgg
     2701 accatagcaa gaggtctcag actgaatgaa gacctcaccg aggcaatagc tctcggacac
     2761 gacctggggc atacaccctt cggacatgcc ggagagaagg tattgaatga aatacatccg
     2821 gtcgggttca agcataatga acaaagcctc agggttgtag atatacttga gggaggaaaa
     2881 ggccttaacc tgacttatga ggtcagagac ggtattttga ggcatacggg agatatcggg
     2941 cctgaaacgc ttgaaggaaa tattgtcagc ttttccgacc ggattgcata tataaaccat
     3001 gacattgatg atgcacttag agggggtata ttggaaatgt cctcacttcc cgagaagtgt
     3061 attgcaatac ttggagatac ccacaagaaa aggattaata caatgataac cgacataata
     3121 acccaaagca tggataaaaa tgagattatg atgagcagta tggtaaggca ggctactgat
     3181 gagcttaggg aattcatgtt tgagaatgta tatatcggtt cggaggccaa ggcggaagag
     3241 aagaaaacca ggaatataat aagctgcttg tatgaatact atgtgcaaaa ccctgaggaa
     3301 atgcccattg agaatatcag ggagaatagg gatgcggata atatagatat agaaagactt
     3361 gcatgtgatt atatcgcagg aatgtccgac cgttttgcca taaacaaatt taccgagctg
     3421 tttattccat ccccctggcg agtatagcag ggaaataata tccataaaat ttattttctt
     3481 ttgaaggata ttaggattat ttgtcgaata aaaactatct tgttacgtta attattaatt
     3541 agaggtatat gtatgagaat attgatagac ggggacgcct gccccgttac gcatttgatt
     3601 gagaaaacag cagcacgatg tggggtcggt gttgtttttt ttcacagcac atgtcattac
     3661 agcaactatt caaatgcggc tgcggaaaaa ataatggttg ataatgaatc acaggcagta
     3721 gatatggcaa taattaataa agcagtaaag ggagatattg tagtgacagg agactttgga
     3781 cttgcatctc tgacccttgg gaagggagcg aaagctatat ctttcagcgg tatgatattt
     3841 gacgaatcca atattgaccg gcttcttttc gaaagacatg tatccggagt aatcaggagg
     3901 agcggcggca gaaccaaagg ccccgcaagg cgtacagaca gtgacgacag gaactttgaa
     3961 aaatcactca gctttctaat ttcacaaatt atgaaggata tttaagataa atggagaact
     4021 attatataaa taattaatga tatcatattt taccgtttga ttaaagtaaa aggtggaaaa
     4081 aaagatgttt ttctcagaag ataagatttc tgagataatt gagaaaaacg atatagtgga
     4141 tgttatttca gaatatatga cattaaagag gagcggcagc aattatatgg ctttgtgccc
     4201 tttccattcg gagaagacac cgtcctttag cgtgtcacca tctaaacaga tatttaattg
     4261 ttttggctgc ggtttcggag gcaacgtaat aacatttatc cagaaaatcg aaagaattga
     4321 atttgtggaa gcagtgaaaa tgcttgctga aaaagccggg gtaaagctgg aagaggacaa
     4381 ggatcccaaa cagttggaaa gagctaagca aaaaaaggca ttgtacagga taaatgctga
     4441 agctgcaagg tttttctaca gcaatctgaa tgccggatcc aaagctttgg agtacctccg
     4501 aagaagaggc ctgagtgatg caatcatcaa aaggttcggc ttgggatatg caccggatga
     4561 gtggtatggg ctgatgaacc atatgaaaag gaaggatatt gataccgagc ttctttttaa
     4621 ggcagggctt attgtcagga ataacgatgg tgagaaattt cgcgacaagt tcagaaacag
     4681 gataatgttt cccataatag atctcaaggg aaatgttatt gcttttggag gaagggtaat
     4741 agatgattca aagcccaagt atctcaactc acctgatacg cttgttttta acaagggcta
     4801 caatatttac ggtttgaatt ttgttaaaaa ggtgcagaac ctggaaaata tagtagtagt
     4861 cgaaggctac atggatgcaa ttgctttgca tcaatttgga gtggagaatg ccgtcgcttc
     4921 tttaggaaca gcctttactg aaaatcaagc taagcttttg aagcgttttt caaatgaaat
     4981 cataatagca tatgattcgg atctggcagg acaggctgcg acattgaagg gattggcaat
     5041 ccttgagaag gaaggctgtg tggtaaaggt actaagcctg ccttcaggaa aggatcccga
     5101 cgagtatata aggaaagaag gcattgatgc ttttagagac agaataagga agagtgtttc
     5161 ccttattgaa tatcagattg aaaatttaaa aagaggtttg gacataaata acatacagga
     5221 cagaataaaa tttacaaaag ggttctccga gattttgaag aacgttgtaa gcaacgttga
     5281 agtagatgcc tatatcagaa agtattccgg tattatgaag gtaaatgagg aggctctgta
     5341 tgcggagctg aacaggctca ggaaaaagaa taaaaatgga aataataaac ataatatatt
     5401 aaatagtata aaaaccgagc cggaattaaa aaaaggagaa ataacagcag agatttatct
     5461 tcttaatata tgtatttatt atttcaagaa agtaaaaaat atttttgaga ttttgaaacc
     5521 ggccgatttt tccaatgata tacatgtcag gattgcagaa atcatccaaa gggcatataa
     5581 ggaggataag gcaataactg caggtgaaat catcagttac tttgataatg atgatgaaaa
     5641 gtcaagagtc gcgggaatct tcagccaaaa actaccggat tcggatgtgg acgggctgat
     5701 taagtcaagc ttggataaga tcagacaatc gaaattggat agaacgattc aggaaatgac
     5761 agaaaaaatg aatgaaatgt atcacgcagg aaaaaaggaa gaagccaata aaatctttgt
     5821 tgagattaaa gagctgcaga agaagaagag gtaatatccg gaagggaggg gatacggtga
     5881 aaacagaaga acttagaatg acatatgtaa aagaattggt tgataaagga aagaaaaacg
     5941 gatcgctgac atataaggaa ataatggaca cttttgaaga agtggaaatg gatcctgaac
     6001 agatagataa gctgtatgaa tatcttgaga ctaaaggcat agatgtggta ggagcaatag
     6061 ataatgatat ggatattccg cctcttgatg aggatgtgga ggaagtcgat cttgatataa
     6121 gcattccgga aggtattagt atagatgatc ctgtcagaat gtatctgaag gaaataggga
     6181 aagtgcccct gctgagcccc gaggaagaga tcgatttggc aaatagaata aagaaaaatg
     6241 attccgaagc caaaaagaaa cttaccgaag caaaccttag gctggtagtc agcattgcaa
     6301 aacgatatgt aggaagagga atgctgttcc tggatcttat acaggaaggt aatctggggc
     6361 ttattaaggc tgttgaaaag tttgactata caaagggatt taaattcagt acttacgcta
     6421 cctggtggat tagacaggca attaccagag ctattgccga ccaggcaagg acaatcagaa
     6481 tacccgtgca tatggttgaa acaataaata agcttataag ggtttcaaga cagctcctgc
     6541 agcagcttgg cagagatcct atccccgagg agattgccgc tgaaatggga atgtcggagg
     6601 ataaggtaag ggagattatg aaaattgccc aagagcccgt ttctctcgaa actcctattg
     6661 gtgaagagga agacagccat ttgggggact tcatacctga tgaagacgct cccgcaccgg
     6721 ccgaatcggc agcatttaca ctgcttaagg agcagcttat ggaggtgctt gatactttga
     6781 ctccgaggga ggaaaaggtg ctgaagctga ggttcgggct ggaggatgga agggccagaa
     6841 ccttggagga agttggcaaa gagtttaatg tcaccagaga gcgtataagg cagattgagg
     6901 caaaagcatt gaggaagctc aggcatccaa gcagaagcaa aaagctaaag gatttccttg
     6961 aataaacaaa atttactgtt gactttcata ataaaagtta gtaaaataat atttgtcagt
     7021 ttaaagttcc tcaatagctc agtcggtaga gcatgcggct gttaaccgca gggtcgttgg
     7081 ttcgagtcca acttggggag ccattttcgg gcctttagct cagttggtta gagcaaccgg
     7141 ctcataaccg gttggtccgg ggttcgagtc cctgaaggcc caccatttat tatgagtata
     7201 aacctgatac gaaagtatca ggttttttac tatccaccta tttgtttccc ccgc
//
LOCUS       DAOQUR010000087         7237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_11367_length_7237_cov_10.591757, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000087 DAOQUR010000000
VERSION     DAOQUR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11367_length_7237_cov_10.591757"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1366)
                     /locus_tag="PKI50_11645"
     CDS             complement(89..1366)
                     /locus_tag="PKI50_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT04392.1"
                     /translation="MIIHVVRAGDSLYSISRRYGVPVERIASDNELNVSESLVIGQTI
                     VVMEGTRRHTVRAGESLYSIARQYGTTVAGIQAANQIANPAMIRPGMVLTIPSGTDKL
                     GTIDVNGYAFPNISMDVLRKTLPYLTYLSIFSYEVKPDGSFRPINDEPLIAAARAANV
                     APLMVITNLREGGGFDSDLAKTILTNEQVQDTLINNVIENLRAKNYTGLDIDFEYVYP
                     ENREDYNNFLRKVSSGLRPLGYSITTALAPKIAADQPGLLYEAHDYPVHGALVDHVIL
                     MTYEWGYTFGPPMAVAPINEVRKVINYAMSAIPRRKILMGIPNYGYDWVLPYVEGSRA
                     TTISNVGAVDLARREGAAIQYDQTAQAPFFNYYDDNGRQHVVWFDDARSMEARLRLVN
                     QYNLGGVSYWTIGRFFPQNWLVLSSLYDVRKVL"
     gene            complement(1615..2868)
                     /locus_tag="PKI50_11650"
     CDS             complement(1615..2868)
                     /locus_tag="PKI50_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNT04393.1"
                     /translation="MNAPLQSKRMLGINGIGRIGKLTLWNHLNLKHFDGIVINAGREV
                     GKKADDIIHYLTTDSTYGTVDRFLYGYTGKSCNVKVLDETECVFEMNGMPVRILRKER
                     NPRNIDWSREGVRLVVDCTGVFLDPTQPSDSPKGSIRGHLEAGAEKVIASAPFKIKDS
                     SQRMPDDSALFVYGVNHASYDPARHHILSAASCTTTGLAHMIKPLLDTEETSRIITAS
                     MSTVHAATNNQNILDAPPKAGSKDLRRNRSVFNNIIPTSTGAAIALEEILPEIKRVGF
                     MADSVRVPTSTVSLISLNITFRTDLNLTGEPIINREFINDIYRKAASGAQKGLLIFSE
                     EQNVSSDLMGCQAAVIIEGSENHTRTGFLPLSSEVLREYGIDTAQDINLPVTHAKIFG
                     WYDNEFGNYVNCLGKLTVYVDKNMI"
     gene            complement(3004..7092)
                     /locus_tag="PKI50_11655"
     CDS             complement(3004..7092)
                     /locus_tag="PKI50_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT04394.1"
                     /translation="MEKSFSSKALEVNLAKTRDTDILIPEEHKWFAELSKSYWGIYKR
                     TQEFIKELNHQYINYQYVIENLHNISLTDLWFYNSLEESEKALAVLVDIFKKLLEANL
                     KENQRELLITTLIKFIDRLAKLGEYPESIIHQCLGIIKADMGKHELLYIRNSGYFKTY
                     LNKIAELPQYNRIVLEITGNLLSKCIDYWEETSEVGKWFQEKRSLFHTDNSDKIKDIG
                     KPFFEGLRMQLSKASDWSRMCEMLFFNDISNYFRRFSEQFDTSIERIYYIFYLIHLPG
                     MLQLKNHLLYDMNRLLRNVLKELDENETTTFLTNIMALFGELKEQHTGTVLECIQTLG
                     KEVIDTGDKRIISYFVKGLIEYGFVYPGELVVNSDWQIQVNTNHVKNIRVWLELIEYS
                     PEDMKELLSSLIVNLKLGGIFISDTDLFQRDVTKLLNSNIEPVYKQMKQLARIFPVYF
                     RDIGAEGKLREVTTAVDELSRRKDKLIHFLRKQIHTESNNTHIELAKKIIQYWYDGNP
                     EPLRKVVPEDVFEFLDTSSEWYIHVHEILTELCRMKDADPEQLLLLDVKELEQSISAI
                     GSESSRDKRRVAYLIQLYLLLLEKYSLESENIVLMLKGYRFFSNKDIEELRQGLDRND
                     IREALGQVYKLMRHLKKIIIDKNTSEALENIYYKRHVAIGIPSMYGQYIEPKFEALGL
                     MFRLEKAASKMMVQLLQDINLEYISAKTFRDVYDVLGLFKEGLELDGIYNQGFNSNFE
                     MFKYSLTSPSFSLDQYIDIFQFMAQNIKQIINEYFLDVYESPLKVAIPQIFDIKGPLS
                     DPKNKQLYHMESERFFREILSSAFLVQDLDNFITNLVSTLRSMVDNYSGDIIKSMMTY
                     DPDLTISPLYMETVEMDNPVFLGAKAYFLKKMISYDFPIPYGFVLTTEVFRHRRIITE
                     HPYMEQEIDQLILNHIWELEKITRQQFGNPKNPLLFSVRSGTAISMPGAMRTFLNVGM
                     NDEIAETFSKKPNHSWTAWDCYRRFIQSWGMAYGIERDVFDGVILEHKVKFGVEQKIQ
                     FTAEQMKEIAYAYKKVLGDYGIHIEKDPFKQLKQAILSVIDSWSSGRAIYYREHLQIA
                     DEWGTAVIVQKMALGNLSERSGTGVVFTNSPLKTKSGINLYGDFTLCSQGEDIVSGLV
                     HTLPITESQRRDYYSDCSISLESAFPDIYNALLDLAKQLIETYGFMHQEIEFTFESEN
                     PDDLYILQTRNQKLKKQKRLSSFIPAPDEMKLAGRGIGIGGGALTGILTFDMEDLKEA
                     ANKHPEEKRILVRPDTVPDDIPLIFSSDGLITGKGGATSHAAVTAASLGKVCVVNCKG
                     LIVNEAEKRCSINGISLKSGDRISIDGNLGNVYEGVYEIQYE"
BASE COUNT         1871 a         1742 c         1350 g         2274 t
ORIGIN      
        1 tgtccccgtc cccatgtctc cgtgacgcat tattcttcta atgtgcagta gaaggaggca
       61 gccttgattg actgtctcct ttgagatgct aaagcacttt tcttacatcg tacaatgagc
      121 ttaggaccaa ccagttctgc gggaagaacc ttccgatggt ccaatagctt acgcccccga
      181 gattgtactg atttaccagc cttaatctcg cttccatgct gcgggcgtcg tcaaaccata
      241 caacatgctg tcttccgtta tcatcataat agttgaagaa tggagcctgt gcggtttgat
      301 catactgtat ggcagcccct tcccttctgg caaggtctac cgctccaaca ttggatattg
      361 ttgttgccct gctgccttcc acatagggca gaacccaatc atagccgtaa ttcggtattc
      421 ccattaaaat ttttcgtctt gggattgccg acattgcata attaatgacc ttccgcacct
      481 cgttgatagg tgccacagcc ataggaggcc caaaggtata gccccattca tatgtcataa
      541 gtatcacatg gtcaaccagt gctccgtgga caggataatc atgagcctca taaagaagcc
      601 cgggctgatc cgccgctatt ttgggcgcca gagccgtagt aattgagtaa cctaacggtc
      661 tgagcccgga agataccttt ctcaggaagt tattgtagtc ttccctgttt tccggataaa
      721 cgtattcaaa gtctatgtca agtccggtgt agttttttgc tctaaggttt tctatcacat
      781 tattaatcag tgtatcctga acctgttcat ttgtcagtat tgtctttgcc aagtcgctgt
      841 caaagccgcc gccctccctc agattggtta tcaccataag gggtgcaaca tttgctgccc
      901 tggctgctgc aatcagaggc tcatcattaa tcggcctgaa gctcccgtcg ggtttgactt
      961 catagctgaa aatactcagg tacgttaagt acggcaatgt ttttctcaat acatccatac
     1021 tgatattcgg aaatgcgtac ccgtttacat caatggtacc cagcttgtct gttcctgatg
     1081 gtattgtcag taccattccg ggtctgatca tagccgggtt ggcaatctga ttggccgctt
     1141 gtattccggc aacagttgtt ccgtattgtc tcgcaattga ataaagcgac tcccctgccc
     1201 tcacggtgtg ccgtcttgtc ccttccataa caacaattgt ctggcctatc accaggcttt
     1261 cagaaacatt taattcgttg tcggatgcaa ttctttccac cggtacccca tagcgccttg
     1321 aaatcgaata taagctgtcc ccagctctta caacatgaat aatcaaatgc catacacctc
     1381 acatatttca agtatgccac cagcatactt gcgcgccgat ttcgtatttc aggtcgtgaa
     1441 ataatcttcc ttgcgaaatc gttcgcatcc gccggaggct ataacatcag ttgagttcat
     1501 ctctactgat gttatactct tatagtatat gttgcggtac atggcattgt tacttcattt
     1561 ttaaaaacaa atgctccggt atttctaccg aagcataaca gtgtatccct aaatctaaat
     1621 catattcttg tcaacatata ccgtcagctt tccgaggcaa ttgacataat tgccgaactc
     1681 gttgtcatac cagccgaata tcttggcatg ggttacaggt aaattgatat cctgcgctgt
     1741 atctattccg tattctctca ggacttccga agaaagaggc aggaaccctg ttctggtatg
     1801 gttctcgctt ccttcaataa ttaccgcagc ctggcatccc attaaatcgg aagatacatt
     1861 ctgttcttcg ctgaatatca ataggccctt ttgtgcgccg gatgcagctt ttctgtaaat
     1921 atcatttata aactctctgt ttattatagg ctccccggtc agattgagat ccgttcggaa
     1981 agtaatgttc aaagatataa gcgacactgt acttgtaggt acccttacag agtcagccat
     2041 aaaccctacc cttttgattt caggaagtat ttcttccagt gctatggcag cacctgttga
     2101 tgtcggaatt atattattga acactgacct gtttctcctc agatcctttg aacctgcctt
     2161 cggaggagca tccaatatgt tctgattgtt tgttgcggcg tgtacggtag acattgatgc
     2221 cgttattatt cttgaagtct cttctgtatc taggagaggc ttgatcatgt gcgccaatcc
     2281 tgtagtagta cagcttgcag ccgacaatat gtgatgtctg gcaggatcat agctggcatg
     2341 attcacacca tatacaaaca aagcgctgtc atcaggcatc ctttgtgaag agtccttgat
     2401 tttgaaaggc gcgctggcga ttaccttctc cgcccccgct tccaaatggc ctctgatgct
     2461 tcctttgggg gaatctgatg gctgcgtcgg atctaaaaat actcccgtgc aatcaacaac
     2521 cagtctgacc ccttctcttg accaatctat attccttgga tttctttcct ttctaagtat
     2581 cctgacaggc attccgttca tttcaaatac acattcagtt tcatccaata ctttcacatt
     2641 gcaggacttg cccgtatagc catacaggaa tctgtcaacg gtgccataag ttgaatcggt
     2701 agtcagataa tgaataatgt catcagcttt cttacccacc tcacggccgg cattgattac
     2761 tattccgtca aaatgcttga ggttgagatg attccataag gtcagtttcc cgatacggcc
     2821 tataccatta atccccagca tccttttgct ttgtaaaggt gcattcattt aatcactcct
     2881 atcccttaat agctttacag tctaattata tcatgtagca catttcttct caatatctta
     2941 aattatgcgt tatatttcct tattgctgct tattgtgata tatcattaaa ttaatacgtt
     3001 atactattca tattgtattt cgtatacacc ttcatataca tttccaagat taccgtctat
     3061 ggatatcctg tctcccgatt ttaagcttat accatttata ctgcatctct tttctgcttc
     3121 atttactatc agacctttgc agttcacaac gcacaccttt ccaaggcttg cggcagtaac
     3181 tgcagcatgg gatgtggccc cccccttgcc tgtgataagc ccgtcactgc tgaatatcaa
     3241 aggtatatcg tcaggcacag tatcaggcct taccagtata cgtttctctt caggatgttt
     3301 attggcggct tcctttaagt cctccatgtc aaaggtcaat atccccgtca acgctccgcc
     3361 gccgatgcct atcccccgcc ctgcaagctt catttcatca ggagccggta tgaaactgga
     3421 cagccgcttc tgtttcttca gcttctggtt cctggtctga agtatataca ggtcatcagg
     3481 attctcggac tcaaaggtga actctatctc ctggtgcatg aagccgtacg tctcaataag
     3541 ctgtttggcc aaatccagca aagcattata tatatcggga aacgcagact ccagagatat
     3601 gctgcagtcg ctgtaataat ccctgcgctg gctttcggta ataggcagcg tgtgcaccag
     3661 ccctgatact atatcctcac cttggctgca taatgtaaaa tctccgtaca gatttatacc
     3721 ggacttggtt ttaagcgggc tgtttgtaaa tactactccc gtaccggagc gctctgatag
     3781 gttgccgaga gccatttttt gcactattac ggctgtcccc cattcgtctg cgatctgcag
     3841 atgctctcta tagtatattg ccctgccgga ggaccatgaa tcaataacgc ttagtatagc
     3901 ctgcttcagc tgcttgaagg ggtctttttc aatatgaatt ccgtaatcgc caagcacctt
     3961 cttataggca taggctattt ctttcatctg ctcggcagtg aattgaattt tctgctcaac
     4021 gccaaacttt actttgtgct caagaatcac tccgtcaaaa acatccctct caattccgta
     4081 agccataccc cagctttgaa tgaagcgcct gtaacaatcc caggcagtcc atgaatggtt
     4141 tggcttcttg ctgaaggttt cggcaatttc gtcattcatt cctacattca gaaaagtcct
     4201 catagcgccc ggcatggaga ttgccgtacc tgaccttacg gaaaacaaca gaggattttt
     4261 tggattgccg aactgctgcc gtgtaatttt ctccaattcc catatgtggt taagtattag
     4321 ctgatcaatc tcctgttcca tatacgggtg ctccgtgata atcctcctat gcctgaatac
     4381 ctcagttgta agaacaaacc cataaggtat cggaaaatca taagatatca tttttttaag
     4441 gaaatatgct tttgctccaa ggaataccgg attatccatt tccaccgtct ccatataaag
     4501 gggacttata gtcagatccg ggtcatatgt catcatactc ttaatgatat cacctgaata
     4561 attgtcaacc atgcttctta aggtgcttac cagattggta ataaaattat ccagatcctg
     4621 aaccaaaaag gcggatgaca gaatttctct gaagaatctc tccgattcca tatgatagag
     4681 ctgcttgttt ttaggatcag aaagagggcc cttgatatca aatatttgcg gtattgccac
     4741 tttcagcggg ctttcgtaaa catcaaggaa gtactcgttt attatttgct tgatattctg
     4801 tgccatgaac tgaaatatgt ctatgtattg atccagtgaa aagcttggag aggtaaggct
     4861 gtacttgaac atttcaaagt ttgaattaaa tccctggttg tatatgccgt ccagttcaag
     4921 cccttccttg aacaatccca atacatcata tacatccctg aaggtcttgg cagatatgta
     4981 ttcgagattg atatcctgaa gaagctgcac catcatcttt gatgcggcct tttccagcct
     5041 gaacatcaat cccagggcct caaattttgg ttcaatatac tgcccgtaca ttgagggtat
     5101 acctattgca acatgacgtt tataatatat gttttccaaa gcttcgcttg tatttttatc
     5161 aataataatt tttttcagat gccgcatcag cttataaacc tgtccaaggg cctctcttat
     5221 atcgtttctg tcaagaccct gacgcagttc ctctatatcc ttattgctga aaaacctgta
     5281 gcccttcaac attaaaacaa tattttccga ttccagagag tatttttcca ataaaagcaa
     5341 gtaaagctga atcaaatagg caactctcct tttatcccgg ctgctttccg aaccaatagc
     5401 ggaaatagac tgctccagct ctttcacatc aagaagcaga agctgttcag gatcggcatc
     5461 cttcattctg cataactccg taagtatttc atgaacatgt atgtaccact cgctgcttgt
     5521 atccaggaac tcaaaaacat cttccggcac aaccttcctt aaaggctcgg gatttccgtc
     5581 ataccagtat tgtattatct tttttgcaag ttctatgtga gtattgttgc tctctgtatg
     5641 tatctgcttc cttaaaaaat gtatcaactt atcctttctc cttgataact catcaactgc
     5701 ggtagtcacc tctctcagct ttccttccgc tccgatgtcc ctgaaataca ccgggaaaat
     5761 cctggccagc tgcttcatct gtttataaac cggttcgata ttggagttta acagcttggt
     5821 aacatctctc tggaaaagat cggtatccga tatgaaaatt cctcccagct tcagattgac
     5881 tataagggac gaaagcagct ccttcatatc ctcaggtgag tattcaataa gttcaagcca
     5941 gactctgatg tttttcacat gatttgtatt cacctgtatc tgccagtcac tgtttacaac
     6001 aagctctccg ggatacacaa atccgtattc tatcaatccc ttgacaaaat aagagattat
     6061 tctcttatcc ccggtatcaa tgacttcctt ccccagggtc tgtatacatt ccaataccgt
     6121 tcccgtatgc tgctccttca attccccgaa caaagccata atattggtaa ggaaggttgt
     6181 cgtctcattt tcatccagtt ccttcaatac attccttaac agcctgttca tatcataaag
     6241 cagatggttc ttaagctgta acattccggg cagatgtata agataaaata tataatatat
     6301 cctttcaatg gaagtatcga attgctcact gaaccgtcgg aagtaattgg atatatcatt
     6361 aaaaaagagc atttcacaca tccggctcca gtcagaggct ttgctcagct gcatccttaa
     6421 cccttcaaaa aaaggcttcc cgatgtcctt tatcttatcg ctgttatctg tatgaaaaag
     6481 tgatctcttt tcctggaacc attttcccac ctctgaggtc tcttcccaat aatcgatgca
     6541 tttagaaagc aaattccccg taatttccag cacaatccgg ttatactgcg gaagctcggc
     6601 tatcttgttc agatatgtct tgaaataccc tgagttcctt atgtataaca gctcgtgctt
     6661 tcccatatcg gccttgatta ttcccaggca ctggtgaata atgctttccg gatattcccc
     6721 cagctttgca agacgatcaa tgaactttat tagagttgta atcagaagtt ctctctgatt
     6781 ctctttaaga tttgcttcca acaatttctt gaatatgtcc accagcaccg caagtgcctt
     6841 ttcggactcc tccagagaat tataaaacca caggtctgtc aggctgatat tatgcagatt
     6901 ctcaattaca tattgatagt tgatgtattg atggttaagc tctttaataa attcctgagt
     6961 gcgtttgtat ataccccagt atgacttgga aagctccgca aaccacttat gctcttcggg
     7021 aataagaata tcagtatctc gtgtttttgc aaggtttacc tccagagctt tagaagaaaa
     7081 acttttttcc atttggtcat cctccagaat ttaatattaa gatgtagttt gctgcaaaat
     7141 ataaaactat gatatgagta tattatatca taaatttgta aaaatttatg attgtacatc
     7201 tgaatgagac atggggacag ggacacgggg acgtttc
//
LOCUS       DAOQUR010000088         6959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_11961_length_6959_cov_10.335458, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000088 DAOQUR010000000
VERSION     DAOQUR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11961_length_6959_cov_10.335458"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..839)
                     /locus_tag="PKI50_11660"
     CDS             complement(348..839)
                     /locus_tag="PKI50_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04395.1"
                     /translation="MANIKINLNGLEIMVFFWNSLMSREKVSESFLNDLCSMQEFSYI
                     YNEDFNNESLRRLLSALNNKEPFSGNKPERKYYSNSLMIMEYIDEVQPAINAIKKLNL
                     NSIAEKAKEDVEVVVVPGLSKGIEKTDNKLIVDFFSLKNTENGLVISGESLEAVLKNM
                     FEV"
     gene            1110..1901
                     /gene="arsM"
                     /locus_tag="PKI50_11665"
     CDS             1110..1901
                     /gene="arsM"
                     /locus_tag="PKI50_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1722..1724,aa:Sec)
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNT04396.1"
                     /translation="MAKIKTDIRNAVRRSYGRIASGRAKDEGCCGSNITVKKSAKDIS
                     RGIGYSEEEMEEIPEGADMGLGCGNPQLIAEIKEGETVIDLGSGGGIDCFLASRKVGP
                     KGYVIGVDMTPEMLIKSRILARKHRYSNVDFRLGEIENLPVADNTADVIISNCVINLS
                     PKKQRVYDEAYRVLKKGGRIAISDVVLVKELSNEMKQDEKLYCGUVAGASSVEELKAY
                     LEKAGFSNINIDTKEVSEEYAAKWSHNLKVGEYIMSASIKAVKAK"
     gene            1927..3108
                     /locus_tag="PKI50_11670"
     CDS             1927..3108
                     /locus_tag="PKI50_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT04397.1"
                     /translation="MPELSSKVNIFTDSVIRRMTRISEAYGAINLSQGFPDFDPPKEI
                     LDALAYAAYNGPHQYTFTYGSYNLRSALAKKHGKAINRTIDPDMEVLVTCGGTEAMMS
                     AMMTICNPGDNVIIFSPYYENYRADTILSGANPIIVPLNPPDYSFDKQVLEDAFRNNV
                     KAIIVCNPSNPTGKVFTTEELEFIGHLALRYDAYVITDEVYEHIIYEPFHHVHMAGLP
                     DMFEHTITCSSLSKTYSITGWRLGYLIGPEKVIESTKKVHDFLTICAPAPLQEAACVG
                     VNFGDSYYKDLQRKYTEKRNYFCRRLDEIGLRHNTPQGTYFVMMDISEFLSMDKFKGW
                     TDLQFCEWMIMEIGVAAVPGSSFFMEPVNNLIRLHFARSMDTLDEVLNRLEKLGRYLH
                     R"
     gene            3144..3989
                     /locus_tag="PKI50_11675"
     CDS             3144..3989
                     /locus_tag="PKI50_11675"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNT04398.1"
                     /translation="MRELNVDVITNTVENLCIESNYFLPRDVKKALEDAVLKEESPVG
                     RETLLDILKNAEIAARNQVPICQDTGLTVVFAELGQDLRLVGGDFYEAINEGVRRGYK
                     NGYLRKSAVDDPFLVRKNTGDNTPAIIHTTIVKGDRLKIIIAPKGGGSENMSALRMLT
                     PAEGVDGIKKFVIDTVDKAGSNPCPPVIVGVGIGGTVEMTALIAKKALLRTVGEHNPN
                     TEVAKLENELLEEINNLGIGPQGFGGRITALAVNIETFPAHLASMPVAVNIQCHAARH
                     KEAVL"
     gene            4012..4560
                     /locus_tag="PKI50_11680"
     CDS             4012..4560
                     /locus_tag="PKI50_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HNT04399.1"
                     /translation="MSRIINTPLTDEVTCNLHAGDRVLINGIIYTGRDAAHKRLTELL
                     DRGEKLPFELNGQVIYYVGPCPAKPGNVIGSAGPTTSCRMDVYAPGLMEMGLKGMIGK
                     GSRSKDVIDSIVRNKCVYFAAVGGAGALLAEAIKEAEVVAFADLGPEAIFKLKVENFP
                     AVVIADTEGKDLYKIGRSKYQR"
     gene            4573..5805
                     /locus_tag="PKI50_11685"
     CDS             4573..5805
                     /locus_tag="PKI50_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNT04400.1"
                     /translation="MMSLREDALKLHRDNRGKLQVVSKVPVNDKESLSLAYTPGVAEP
                     CKEIQKDPDKVYEYTFKGNMVAVVTDGTAVLGLGDIGSHAALPVMEGKAVLFKNFGGV
                     DAFPICLNTIDADKIVESVTLIEPAFGGINLEDIAAPACFEIENKLKKKLSIPVFHDD
                     QHGTAIIVLAGLLNSIKITGKKLQELAVVINGSGAAGIAITKILLSAGVKDAILCDRA
                     GIIYKGRNTGMNWAKEEIALVTNKECRKGTLADAMVNSDVFVGVSSANIVSEDMVRSM
                     NKDAVIFAMANPVPEIDPEPAKKAGARIIGTGRSDYPNQVNNVLAFPGVFRGALDVRA
                     SEINEEMKLAAAYALSEIIPADELTEDYIIPKVFDPRVAPAVAKAVAKAAIETGVARA
                     AVDPEQVALRAAGLIKLK"
     gene            5830..6255
                     /locus_tag="PKI50_11690"
     CDS             5830..6255
                     /locus_tag="PKI50_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNT04401.1"
                     /translation="MVIKQTIIRGINNALKVILDLIKYIIPAVFVLKVLEHSGWLVRI
                     ADLFGPFMSYIGLPGEGALIIVMGQVTLYSAIAAMAVVDITVKQLTIVSTFVTIFHSF
                     ILETAVINKAGGNGFMIIGIRVIAAVLVCFILNILIPGV"
     gene            6280..6738
                     /locus_tag="PKI50_11695"
     CDS             6280..6738
                     /locus_tag="PKI50_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04402.1"
                     /translation="METFEVSFSTIFKMTVIVVPLLIGIEYLKEIGWMEKLSVKLRKV
                     SELIGLPGEAAFGMIVVYFIGLVFGSGVIIRMKKEVGMTRKQLNTMFIFIGICHAVVE
                     ETIVFTAVGANGVIVMVSRVLASFLFGLLYAWFAKHDETSIRKSSGIELE"
BASE COUNT         2104 a         1228 c         1691 g         1936 t
ORIGIN      
        1 tcaattttac tcattctatt atcaattgta cccattctat gttcaagctt attttgtccc
       61 gactccagat tgtcaagccg ctgggaatta ttttttacct ctttcttagt gtcctgcagc
      121 tctacatata ccttttccat aagatcatag accttatcaa tatcactcat gaggtttcct
      181 cctaaaatac attcctgatg atatttagtt taccacaaac cggaagaatt tagaagtaaa
      241 atcatataag atactgtaaa gaagcatggg aagcgcagct gcaatcgtag acacccgccg
      301 gctgctgaca gttgcatgca agataagtgc ggtatataca ataataatta tacttcaaac
      361 atgtttttca aaacagcttc caggctttca ccggatataa ccaatccatt ttcagtattt
      421 tttaagctga aaaagtctac tatcagttta ttatcagttt tttcaatacc tttacttaaa
      481 cccggtacaa ccactacttc aacatcttct tttgcttttt ctgcaatact gttaaggttc
      541 agctttttta tagcatttat ggcaggctga acctcatcta tatattccat tatcataagg
      601 ctattggaat aatactttct ttcaggtttg tttcccgaga atggttcttt gttgtttaat
      661 gcgctcaata atcttctgag agattcgttg ttaaaatcct cattatatat gtatgagaat
      721 tcctgcatgg aacagagatc attcaggaag ctttcactga ctttttccct ggacatcagt
      781 gaattccaaa agaagaccat gatttcaagt ccgttcaagt tgatttttat gtttgccata
      841 tcaacacctc tgtttatatt tgttcttcct ttgtagaaga cttacattat tataactcaa
      901 aactccgttg ctttccaccc caactttgcc atcaatacct gcaggtttaa tatttgtttt
      961 gaagttataa ttatgcaatc aaaagtaatt ctgttataat aaaagatggt gaaggtttag
     1021 tcttaggata gcaattaagt attagcatta ccatatacag taatatgcaa aaagagaaaa
     1081 aggtattttt agacaagagg tgatattttg tggctaaaat aaagactgac attagaaatg
     1141 cagttcgcag aagctatggc agaattgcct ccggcagggc aaaagatgag ggctgctgcg
     1201 gcagtaatat cactgttaaa aaatctgcaa aagatatatc aagaggaatc gggtattccg
     1261 aagaagaaat ggaggaaatt ccggaaggcg ccgacatggg gctgggctgc ggaaatcctc
     1321 agctaatcgc agagataaaa gaaggcgaaa cagtaattga tcttggaagc ggcggaggaa
     1381 tagattgttt ccttgcatca aggaaggtag gccccaaagg gtatgtaatc ggggtggata
     1441 tgactcctga gatgttgatt aaatcaagga ttttagcaag gaagcacaga tattccaatg
     1501 tagatttcag actgggagaa attgagaacc ttccggtagc cgataatacg gcggacgtga
     1561 ttatctcaaa ttgtgttatc aatttatccc ctaaaaagca gagggtatat gatgaagcgt
     1621 accgggtgtt aaaaaaaggc ggcagaatag caatttccga cgtggttttg gtcaaggaac
     1681 tttcaaatga aatgaagcag gatgaaaagc tttactgcgg gtgagtcgcc ggtgcttctt
     1741 cagttgaaga actcaaggca tatttagaga aagccggatt cagtaatatt aatattgata
     1801 ctaaagaggt atctgaggaa tatgccgcaa aatggtcgca caacttaaaa gtcggagagt
     1861 atattatgtc cgcatcgatt aaggcggtta aggctaaatg aatgaaggaa tcaaggaggt
     1921 attgaaatgc ctgaattaag cagcaaggta aatatattta ccgattcagt cataagacgt
     1981 atgaccagga tttccgaggc ctacggggca ataaaccttt cacaggggtt tcctgatttt
     2041 gaccccccca aggagatttt ggatgcattg gcttatgcag catataacgg ccctcatcaa
     2101 tatacattta catacggttc ctacaatctt agaagcgctt tggcaaaaaa acacgggaaa
     2161 gctattaatc gaaccattga tcccgatatg gaggtgctgg tcacctgcgg cggcacggaa
     2221 gcaatgatga gtgctatgat gacaatctgc aatccggggg ataatgttat tatattcagc
     2281 ccctattatg aaaattacag ggcggacacg attctgtcgg gggcaaaccc gataatagtt
     2341 cccttgaatc ctccggacta tagttttgac aaacaggttt tggaagatgc tttccggaat
     2401 aatgtgaagg caatcattgt atgcaatccg tctaatccga cgggtaaggt atttacaaca
     2461 gaagaactgg agtttatagg acatttggca ttgaggtatg atgcatatgt aattaccgat
     2521 gaagtctatg agcatataat atacgagcct tttcatcatg ttcatatggc aggccttcct
     2581 gatatgttcg aacatacgat aacctgcagc tccctgtcca aaacctattc tattacaggc
     2641 tggcgccttg gatatctgat tgggccggaa aaggtaattg aaagcactaa aaaagttcat
     2701 gattttctga cgatatgtgc tccggcaccg ctgcaagaag ctgcatgcgt cggtgtaaac
     2761 tttggggatt catattataa agatcttcaa aggaaatata ccgaaaagcg aaattatttc
     2821 tgcaggcgcc tggatgaaat aggtttacgg cacaatacgc ctcagggcac ttattttgtt
     2881 atgatggata tttcagaatt cttatcaatg gataagttta agggatggac tgacctgcaa
     2941 ttctgtgaat ggatgattat ggagatcggt gttgctgcgg tgccgggttc aagctttttc
     3001 atggaacctg tcaataattt gatacggctg cattttgcaa gaagcatgga tactttggac
     3061 gaagtgttga accgtcttga aaaattaggc cggtatctgc acagatagaa aaaatatacg
     3121 tggtatgcag ggaggaaaac cggatgagag aacttaatgt tgatgtaatc accaatactg
     3181 ttgagaattt atgtatcgaa tcaaattact ttcttccccg ggatgtaaaa aaagcccttg
     3241 aagatgcggt cctgaaggag gaatcgcctg ttggaaggga aacgcttctt gatattctta
     3301 agaatgcgga aatagctgca aggaatcaag tgcctatttg ccaggacaca ggcttgacag
     3361 ttgtttttgc tgaacttggc caggacttga gactggttgg aggagatttt tatgaagcga
     3421 ttaacgaagg tgtaaggcgc gggtataaaa acgggtattt gaggaagtct gcagttgatg
     3481 acccttttct ggtgagaaag aataccggcg acaacacacc ggcaattatt catacaacca
     3541 ttgtaaaggg agacaggtta aagattatta tagcgccaaa aggaggagga agcgaaaaca
     3601 tgagtgccct tcgtatgctt acgcctgccg aaggagtcga cggaatcaag aaatttgtaa
     3661 tagatacggt ggataaggcg ggttcaaatc cgtgtccgcc tgtaattgta ggtgtaggta
     3721 tcgggggtac tgtcgaaatg actgcattga ttgccaaaaa ggctttattg cgcactgtag
     3781 gagaacataa tcccaacact gaggttgcca aactggagaa tgagctgctt gaggaaatca
     3841 acaatctggg tataggccct caaggctttg ggggcaggat tacagccctt gcagtaaaca
     3901 ttgagacctt tcccgcccat ttggcaagca tgcctgtcgc agtaaatatc cagtgtcatg
     3961 ccgcaaggca taaggaagca gtactataag cgaagcagga ggtgcaaaag agtgtccagg
     4021 attataaata cacctttgac agatgaggtg acatgcaatt tacatgcggg ggacagggtg
     4081 cttataaacg ggattatata taccgggcgt gatgctgctc ataaaagatt gactgaattg
     4141 ctggatcggg gagaaaaact gccctttgag cttaacgggc aggttattta ttatgtcggg
     4201 ccttgtcccg caaagcccgg taatgtaata ggctctgcag gccctacaac gagctgccgc
     4261 atggatgttt atgcccccgg gctgatggaa atgggcctca aaggcatgat tggaaaaggg
     4321 tcacgcagta aagatgtaat tgattccata gtcaggaata agtgtgtata cttcgcggca
     4381 gtcggcggag caggggctct gcttgcagaa gctataaagg aagcagaggt tgtggcattt
     4441 gcagacctgg gccccgaggc aatattcaaa ttgaaagtgg aaaatttccc ggccgttgta
     4501 atagcagata cggaaggaaa agatttgtac aaaatcggaa gaagtaaata tcaaagataa
     4561 tatggaggaa tgattatgtc attaagggaa gatgcactta aacttcatag ggataacagg
     4621 ggaaagcttc aagtagtaag caaggtgccg gttaatgata aagaaagcct gagccttgca
     4681 tatactccgg gggttgcaga gccctgcaag gagattcaaa aggatcccga taaagtttat
     4741 gaatatactt tcaagggcaa tatggtggca gttgtaacag acggcacggc tgtcctgggg
     4801 ctgggggata tcggcagcca tgctgccttg ccggttatgg aaggcaaggc cgttttattc
     4861 aagaatttcg gcggagttga tgctttcccg atatgcctta ataccatcga tgcagataag
     4921 atagttgaat cagtaactct gatagagcct gccttcggag gaataaacct tgaagatata
     4981 gctgctccgg catgcttcga gatagaaaac aagcttaaga agaagctttc aatacctgtt
     5041 ttccatgatg atcagcacgg aactgcaatc atagtgcttg caggccttct gaattccata
     5101 aagattaccg ggaaaaagct tcaggagctt gctgttgtca taaacggttc aggtgcggcc
     5161 ggtattgcaa ttaccaagat attgctcagc gccggagtta aagatgccat tctatgtgac
     5221 agagccggta ttatttataa aggaagaaat acaggcatga attgggctaa agaggaaatt
     5281 gcccttgtaa caaacaaaga atgccgaaaa ggcacattgg cagatgctat ggtgaattca
     5341 gatgtgtttg tcggagtatc ctcagccaat atcgtaagtg aagacatggt caggagtatg
     5401 aacaaagacg ctgttatttt tgcaatggca aatcctgttc cggaaataga tcctgaaccg
     5461 gcaaaaaaag ccggagcgcg tattatcggt acgggaagat cggactaccc caatcaggta
     5521 aacaatgtac tggcattccc gggtgtattc agaggtgctc ttgatgtacg tgcatccgag
     5581 attaacgagg aaatgaagct ggctgcagct tatgcacttt ctgaaataat tccggccgat
     5641 gaattgacgg aggattatat aattccaaag gtttttgatc cgagagttgc cccggctgtt
     5701 gccaaagcag tagcaaaggc tgcaatagaa acaggagttg caagagcggc agtagatcct
     5761 gaacaggttg ccttaagggc tgcggggtta ataaaactaa aataaaagct gatgtgaagg
     5821 aagtgtacta tggttataaa acagactatt atacgtggaa tcaataatgc cttaaaggtc
     5881 attctggatc ttataaaata cattattcct gcagtttttg ttctgaaggt attggagcat
     5941 tcgggatggc tcgtcaggat tgcggacctt tttggaccgt ttatgagcta tataggattg
     6001 cccggagaag gagcactgat tattgtgatg gggcaggtaa cattatatag tgcaattgct
     6061 gctatggctg tcgtggacat tacagtaaag cagttaacca ttgtaagcac ctttgttacc
     6121 atatttcatt cattcatatt ggagacagcg gtgataaaca aagcaggcgg aaacgggttc
     6181 atgataatag gaataagggt tattgcagca gtactggtat gcttcatatt gaatatatta
     6241 atcccggggg tatagaagat gaattgggcg gaagttttta tggaaacatt tgaagtcagt
     6301 ttttcaacaa tatttaagat gacggttatt gtagttcctt tgcttatcgg catcgaatat
     6361 ctcaaggaaa taggatggat ggagaagctg tccgtaaagc ttagaaaggt ttcggagctt
     6421 atagggctgc cgggagaagc cgcttttggg atgatagtag tatacttcat cggactggtt
     6481 ttcggatcgg gagtaataat taggatgaaa aaagaggttg gaatgacaag gaaacaactt
     6541 aataccatgt ttatatttat tggcatatgc catgccgttg ttgaagagac catcgtcttt
     6601 acagcagttg gtgcaaatgg ggtaatagtg atggtgagca gggtgcttgc ttcattttta
     6661 tttggcttgt tgtatgcttg gtttgctaaa catgacgaaa cttccataag gaaaagctct
     6721 ggtattgagc ttgagtgaaa aaatatgtta ttataatttt aattaaattt gcaggaggat
     6781 gttacagtga aaaaaggcag tattatatgg atcattgcgt tgctgttgtg gatattggtt
     6841 ctggcggttc cggctacccg gtcgacattt atggctgcta ccgacgccca tccttatgta
     6901 atgggattca tcaagtttgc catactggca actatgggag atttgctcgg aataagagt
//
LOCUS       DAOQUR010000089         6950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_11986_length_6950_cov_11.260769, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000089 DAOQUR010000000
VERSION     DAOQUR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11986_length_6950_cov_11.260769"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PKI50_11700"
     CDS             complement(<1..1346)
                     /locus_tag="PKI50_11700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT04403.1"
                     /translation="MRSVKTLVYTALILGIALYLRLRFIIYAPFEFKIDAWSMYNYAL
                     NVMNGEYYGHSFGEHWSRYAYWPPLYIFLSGFVYRFFGTAQDFIVMRVIQAGFSAVSC
                     LLCFITAREALRDYSDLHEEAGLTAGILMALNTRMVAYTNHLYAETVFITIYLAAVYF
                     SLRYFKDEMSLKLSCPNGSVRVKHLVLFSIFFGLGNLTRPVLLLLPAVIFLSALLFIT
                     ARRFSICRGIKVLAADGALSLLIILAVLSPWILRNYAVTGRLILIDTNGPINFYIAHN
                     PHSNGTWVDIKKAQSDMLKLYETGYKEGLVYIRNNINREIDLLKVKQQLFLYHGDYHI
                     SEAATHLDRTYKLPLYGSLIKMECPYRLPQISFEFLWKSALLLLVLFIFRLISVKSLF
                     ILFCEPLWIIGNFLYMNLIILIFYYAPRYRITAEPFLCILIGILLAGIFTSYRKNNA"
     gene            1656..2033
                     /locus_tag="PKI50_11705"
     CDS             1656..2033
                     /locus_tag="PKI50_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT04404.1"
                     /translation="MDITVSNSTKEPIYEQVANQIKKQIINQQLKPGEALPSIRKLAT
                     DLHISVITTKRAYDELEKEGFIETVIGKGCFVSRENKEFIREKKIRMVEEKLSDVIKD
                     SRVLNIGLQGLKMMLEVLYREGI"
     gene            2257..3849
                     /locus_tag="PKI50_11710"
     CDS             2257..3849
                     /locus_tag="PKI50_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HNT04405.1"
                     /translation="MKKYRIAPKAVKPMSTIKFRTKASAGGNNADSSDFFDRTADRLR
                     KDLSGNADVIMRELFIGGNENIDALLVYLMGNIDKKLLSDSVIKPLMTCGGLGDIEAR
                     GERLKEHITKKCLQTGSVKYSKDYNTALQDMFSAEGILLVRGIEGFIIFTVAGGQKRQ
                     ISEPETEKRIRGTKEGFIEDIWVNIGMIREFIKDPMLKIEMLKIGQRSQTDVAIIYIN
                     GLADSKIVDEVKKRINKINIDAILSSGYVEQYIEDNPWSVFPQLLGTEKPDKAVANLL
                     EGKLLIITDRSPFVFTAPAVFIEFFQATEDYYEKFHAGIFSRIIRLIAFLIAVLGTSI
                     YIALTSFHPELIPSELLISFARLRKKVPFPPVIETLLMEFTVEILREAGIRLPTSVAG
                     TLGIVGGIILGDAAIRSNLVSPAMIVVIAITTVCNFVMPNYSMTLSMRLLKLFLILLS
                     AVFGAYGIALGLLVIVTHLARLESFSVPYLSPYAPFRFSDMKDALLRLPLWMHVKRPV
                     SIPHMDDKRAVNNREKSGTGEN"
     gene            3839..4945
                     /locus_tag="PKI50_11715"
     CDS             3839..4945
                     /locus_tag="PKI50_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HNT04406.1"
                     /translation="MKTEMSYTITSKQLLFTLISCMLGAGILSLPRVTAEAVGQDAWL
                     AVILGGIIPLVSIWLSNKVVSRHPGLSFAALTEKLAGKWAGKLLAAVFVTYSVIASAA
                     ILRVFTEVITIFLLPNTPILVKILLGLAVSAYMASGGIKVLGRLNEFLFYVLLPPLFL
                     SIPAVVKYADLHYLMPVLNNNLAEYSKAAFTTGFAYSGFEAIIVFAPYVLKKKETLKA
                     AVLALIITIMIYLYAVITTVTVFGPELTQMFTWPTLRLLSVTEIPVLERIEFIAIIAW
                     IGVAIRPVSNQYFCAGLIIKDAFGIKSLSKVVALLYPIIAAIAWYPKNIGIVFRLSDF
                     VGMYSLILGTALPLLLLGLSLIRGKGRARDEKKT"
     gene            4929..6065
                     /locus_tag="PKI50_11720"
     CDS             4929..6065
                     /locus_tag="PKI50_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HNT04407.1"
                     /translation="MKKRHRYILLSSVIIINALFLAGCWDERELDELVIPIIIGYDYA
                     NEEEKEQPDDKYLISAGFPIFYEDTLQKYHVDITSGSMEGEVVNRRNTHFGEQLIPGQ
                     LQVVVLGEELISNENIIELTDILSRNPAVKGAIPMAVSKGKAADMLSVPVKQYPNPGI
                     YIQKLIKNIRRTNFFPTCTLFESNRHIISNHTAPLLPYIVHENGNIVFAGSCLLNGGK
                     RIVHLDREKTETAVMLRGIRSRGMITYDIIENNKVIGQITFEGKNKRKVKVKKEGDRY
                     IFNILIKLSGRLYERKKNEPVIDHKDFIKLCQKSLEDLIRKRAEDFVTIVQEEYGFDA
                     LELGIEIKGHSREKISKEDIDRIIQESEISVEVKVCIDNAGGKI"
     gene            6312..>6950
                     /locus_tag="PKI50_11725"
     CDS             6312..>6950
                     /locus_tag="PKI50_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT04408.1"
                     /translation="MLTDILIKSFIKDKDNIRDKSVRQKYGYLGGFVGIACNVMLSGA
                     KIAIGLMINSIAITADAVNNLSDAASSIIMVIGFKITSKPADREHPFGHGRIEYISAM
                     VVSFMVILAGFEFFKGSFDRIRNPETVKFEIIPFSVLLLSIGVKVWLSRFYKKISREI
                     DSKAMEASAADSLSDVTATSVVALSLLASLWTTFPLDGYVGLLVSALIIYSGI"
BASE COUNT         2082 a         1336 c         1688 g         1844 t
ORIGIN      
        1 gcgttattct tcctatacga agtaaatatg cccgccagca ggattcctat gagaatgcag
       61 agaaagggct ccgcagtaat tctgtacctt ggagcataat aaaaaatcag aatgataagg
      121 ttcatataca ggaagttccc tattatccat aaaggctcgc aaaatagtat gaacagggat
      181 ttgacagata taagcctgaa tatgaaaagc accaggagca agagagcaga tttccatagg
      241 aattcaaagg atatttgagg aagcctgtaa gggcactcca tcttaatcaa gctgccgtaa
      301 aggggaagct tataggttct gtccaaatgg gttgcagcct cagagatatg ataatctcca
      361 tgataaagaa agagctgctg cttaaccttt aggagatcta tttccctgtt aatattattt
      421 cttatgtaaa ctaaaccttc cttatagccc gtctcataca gctttaacat atcggattgt
      481 gccttcttta tgtcaaccca tgtcccgttg gaatgaggat tatgagcgat gtagaagttg
      541 atggggccgt tggtatctat cagtattaag cggcctgtaa cggcatagtt tctgaggatc
      601 cagggagaga gcacagcaag aattataagc agtgaaaggg ctccatccgc tgcgaggacc
      661 ttaatgcccc ggcagatgct gaatcttctg gcggtaataa acaacaatgc agataaaaat
      721 atgacagcgg gaagcagaag aagcacgggg cgtgtaaggt tgcccagccc gaagaaaatg
      781 gagaacaaaa caaggtgttt aacccgcacg gaaccattgg gacaactgag tttgagggac
      841 atttcgtctt taaaatacct gagggagaag tatactgccg ctaaataaat agtgatgaat
      901 accgtttcag cgtacaaatg gttggtatag gccaccatcc ttgtattcag agccatcagt
      961 atgcctgcag taagcccggc ttcctcatgg agatcgctgt aatcccttaa agcctccctg
     1021 gctgttataa agcagagaag gcaggaaacg gcggaaaaac ctgcctgtat tacccgcatt
     1081 acaataaaat cctgcgcagt gccaaaaaac ctatagacaa aaccggaaag gaagatataa
     1141 agaggaggcc agtaggcata tcttgaccaa tgttctccga agctgtgtcc gtagtattcg
     1201 ccgttcatta catttaaggc atagttatac atactccagg cgtcgatttt gaattcaaaa
     1261 ggagcatata ttataaagcg aagccttaaa tacaatgcaa ttcctagaat cagagcagta
     1321 tatacaaggg ttttgacact gcgcatcaat tgttttcctc cctatttttg aatcatgtac
     1381 tttttacaat atatttacgc atatatgtac atacattata ctaatttaac cccgcttata
     1441 ttattcattc gcatctttct acatctttca ccaattttat tgacacaaag gtcaaaaatg
     1501 tctatactgt acatatatgg catacacagt atgaacagta tgcacactat aagcagaata
     1561 cacaatttca ggacttcatg tgtatatagt catctatgac agatgggaca aatcctgtct
     1621 cgcagtcagt tttcaaagag gcgaggtgta tatatgtgga tataacagtc agcaattcga
     1681 cgaaggaacc catatatgag caagtagcga accagataaa aaagcagatt atcaatcagc
     1741 agctgaagcc cggggaagct ctgccctcca tcaggaagct ggcgaccgat ttgcatataa
     1801 gtgtgatcac taccaagaga gcctatgatg agcttgaaaa ggaaggcttt attgagactg
     1861 tcataggaaa gggatgcttt gtttcaaggg agaataagga gttcatcagg gaaaagaaga
     1921 taagaatggt tgaggaaaag ctgtccgatg ttataaagga cagccgggta ctgaacatag
     1981 ggctccaggg tttgaaaatg atgctggagg ttttatatag agaaggaata taggaagccg
     2041 caaggcttct tttttatagg aggacgcggg gacatgggga cgcggggaca cggggacgtt
     2101 tcccctgtcc cacttccagt gggacacgcg aaacgtcccc atgtccccgc gtccccgtgt
     2161 ccccgcgtcc ccgtgtctca taataaataa gctttaattg gaaacaataa cttttattac
     2221 agaaaatcag cgcataatca ttgagggaga gtttccatga agaagtacag aattgcccca
     2281 aaagcagtga agccgatgag tacaataaaa ttcaggacga aggcatcagc cggcggaaat
     2341 aatgcagatt cttcggactt ctttgaccgt accgccgaca ggcttcggaa agacctctcg
     2401 ggtaatgcgg atgtgataat gagagagctt tttatcggcg gcaatgagaa catagatgca
     2461 ttgcttgtat acctaatggg gaacatcgac aaaaagctgc tgagcgacag cgttataaag
     2521 ccgctgatga catgcggcgg tctgggagat atcgaggcga ggggtgaaag gctgaaggag
     2581 catattacaa aaaaatgcct tcagacaggc agcgtaaaat acagtaaaga ttataacacc
     2641 gccctgcagg atatgttttc cgcagaaggg attctccttg taagaggaat agagggcttc
     2701 attatcttta ctgtcgccgg cgggcaaaaa aggcagatat cggagcctga aacagagaaa
     2761 aggatacgag gcaccaagga aggtttcata gaagatattt gggtaaatat cgggatgata
     2821 agagaattta tcaaagaccc gatgcttaaa attgagatgc tcaagatagg gcagagatcg
     2881 cagactgatg tggcaattat atatataaat ggtttggcgg attccaaaat agtggatgaa
     2941 gtaaaaaaga ggataaataa aataaatatt gatgccatac tttccagcgg ttatgttgag
     3001 caatatattg aagacaaccc gtggtctgtt ttcccccagc tcctgggaac ggaaaagccg
     3061 gacaaggcag tggcgaacct gctggaggga aagcttctca taataaccga caggtcgccc
     3121 tttgtgttca cggctccggc agtattcatc gagttctttc aggcaaccga ggactactat
     3181 gaaaaatttc acgcgggaat attttcacgg attatccgtt tgattgcctt cctgattgca
     3241 gttttaggca catcaatata tatagctcta accagctttc atccggagct tataccttcg
     3301 gaactgctta taagctttgc ccgattgaga aaaaaggtgc cttttccgcc tgtaattgaa
     3361 acattgctta tggagtttac agttgaaata ctgagggagg cgggtatacg gcttcctacg
     3421 tccgtggcag ggactcttgg catagtcggg gggataattc tgggagacgc cgccataagg
     3481 tccaatctgg tgagccctgc aatgatagta gttatagcaa tcactacggt atgcaatttt
     3541 gtgatgccca actacagtat gacattgtcc atgcggctgc tgaagctttt cctgatttta
     3601 ttgtccgctg tattcggtgc ctatggaata gctctggggc tccttgtaat agtgacacat
     3661 cttgcgaggc tggagagctt tagtgtgcca tacctttctc catacgcacc cttcaggttc
     3721 agcgacatga aggatgctct gcttaggctg cctttgtgga tgcatgtcaa aaggcctgtg
     3781 tcaatcccac atatggatga taaaagggca gtaaacaaca gagagaagag tggtaccggt
     3841 gaaaactgaa atgagctata caattacaag taagcagctc ctcttcacac ttataagctg
     3901 tatgctgggg gcggggattc taagtcttcc cagggtcacg gcagaggcag tgggacagga
     3961 tgcgtggctg gcagtaattt tgggcggaat tatccctttg gtgtctatct ggctttcaaa
     4021 caaagtagta agcagacatc cgggcttatc ctttgctgca cttacggaga agcttgcggg
     4081 aaaatgggca ggaaagcttc tggctgcagt atttgtaaca tattctgtca ttgcttcggc
     4141 agcaatactc agggtgttta cggaagtaat cacaatattt ctgcttccga acaccccaat
     4201 tcttgttaaa atattacttg ggcttgctgt aagcgcttat atggcaagcg ggggaatcaa
     4261 ggttctggga agattaaatg agtttctttt ttatgttctg cttccgccgc tttttttatc
     4321 aataccagca gtggtaaaat atgcggatct tcattatctt atgcctgtgc tgaataataa
     4381 tctggccgag tattccaagg cagcctttac taccggcttt gcttattcag gcttcgaggc
     4441 tattattgta tttgctcctt atgtattgaa aaagaaggag accctgaagg cagccgtgct
     4501 ggcactaata ataacaataa tgatttacct gtatgcggta attaccactg taaccgtgtt
     4561 cgggccggaa cttactcaaa tgttcacatg gcccaccctg aggcttcttt ccgttacgga
     4621 aattcccgtg ctggaaagga tagaatttat tgcaataata gcatggatag gagtggccat
     4681 aaggcctgtc agcaatcaat acttctgtgc cggccttatt ataaaggatg catttggcat
     4741 aaaatccctg agcaaggttg tggcactgct atatcctatt attgctgcaa ttgcatggta
     4801 tcccaaaaac ataggcattg tatttcgcct atcagatttt gtaggtatgt attcgctgat
     4861 actgggaact gcactgccgc tgcttttgct ggggctttcg ctgatccggg gcaaagggag
     4921 ggcaagagat gaaaaaaaga catagatata ttttattgtc ctcggttata attatcaatg
     4981 ctctattctt agcgggctgt tgggatgaaa gggagttgga tgagcttgta attcctatca
     5041 taataggata tgactatgcc aatgaagagg aaaaggagca gcctgacgac aaatacctta
     5101 taagcgcagg gtttccgatt ttctatgaag atacgctgca gaagtatcat gttgatataa
     5161 cttccggttc tatggaagga gaggtagtaa acagaagaaa cacccacttc ggtgagcagc
     5221 tcataccggg acagcttcag gtagttgtcc tgggggaaga acttatcagt aatgagaaca
     5281 ttattgaact gactgatata ttatcaagga atccagcggt taagggagcc attccaatgg
     5341 cagtatcaaa gggaaaggct gctgatatgc tgagtgtgcc tgtgaagcag tatcccaatc
     5401 cgggcatata tatacagaag cttattaaaa atataaggag gactaatttt ttccccacct
     5461 gtacgctgtt tgaaagcaat aggcatatta tttcaaacca tacggctcca ttgctgccct
     5521 acattgtgca tgagaatggg aatattgtgt ttgcaggctc ctgccttcta aatgggggaa
     5581 aaaggattgt gcacctagac agggaaaaaa ccgaaactgc tgtaatgctg aggggaatcc
     5641 ggagcagagg gatgattacc tatgatatta tagagaataa taaggtcata ggccagataa
     5701 cctttgaagg caagaacaag cgtaaggtta aagtaaaaaa agaaggagac aggtatattt
     5761 tcaacatatt gataaagcta tcgggaagat tatatgagcg taagaaaaat gagcctgtta
     5821 ttgatcataa ggattttatc aagctctgcc agaaatccct ggaggattta atcaggaaaa
     5881 gggcggagga ttttgtcacc atagtgcagg aggagtacgg tttcgatgca ctggaactgg
     5941 gaatagagat aaaaggacat tccagggaaa agatatctaa agaagatatt gacagaataa
     6001 ttcaggagtc ggagatcagt gttgaggtca aggtatgcat agataatgcc ggaggcaaaa
     6061 tatagcgttc tatttttctt ttgattttgt tctcgtatag gaaagtataa tttcctatac
     6121 gaggggggat gcaaggggga gaagccccct gccgttcaag gaaggtgctc agctgcagag
     6181 cagcgtcgaa gggaaccata gggttcccta gcgaaaaaag agcgtcgtga gactggcttt
     6241 ttttctgcca tagccgtata atatataaat atggaaacta tcgcattata tggagaatcc
     6301 ggggtgttat attgcttaca gatattttaa taaagtcatt tataaaggat aaagataata
     6361 tcagggataa aagtgtcagg caaaaatacg gctacctggg aggtttcgta ggtattgcct
     6421 gtaatgtgat gctttcaggg gcaaagattg caataggcct tatgataaac agcatcgcta
     6481 ttaccgccga tgcggttaac aatctttccg atgccgcctc ttccataatt atggtaattg
     6541 gttttaagat aaccagcaag cctgccgacc gggagcatcc cttcggccat gggaggattg
     6601 agtacatatc ggctatggtg gtttccttca tggtaatact tgcaggtttt gagtttttca
     6661 aaggctcctt tgacaggata aggaatcccg agacagtcaa gtttgaaata ataccctttt
     6721 ccgtattgct tttatccata ggtgttaaag tctggcttag caggttttat aaaaagataa
     6781 gccgggaaat agacagcaaa gccatggagg cctctgccgc agacagcctc agtgatgtaa
     6841 cggccacatc ggttgttgcc ctgtcactac ttgcttccct ttggacaact tttcccttgg
     6901 acggatatgt ggggcttctg gtatccgcat taataattta ttccggaata
//
LOCUS       DAOQUR010000090         6847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_12216_length_6847_cov_9.487338, whole genome shotgun sequence.
ACCESSION   DAOQUR010000090 DAOQUR010000000
VERSION     DAOQUR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12216_length_6847_cov_9.487338"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PKI50_11730"
     CDS             complement(<1..700)
                     /locus_tag="PKI50_11730"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT04409.1"
                     /translation="MSEVKKAVYYISTNEGRPGTVSRVVWEILNDKGLLKPAGMSFDN
                     REVMMHRDGAGNEYYFAQSEVPVCWDYPRYLAEMNERFADADFSGMVTWHEGASAPPK
                     VLTVHTIGDVNAGVYGPANPAYMRNILISMEHNRVMLGLSDYMTVTEATHWSGTAVSG
                     SSPELILHYPVPMVDIEVGSEEDSWNNRTACEALAGALTHVFESDGKRVHNLLCVGGI
                     HFDPNFAQAVFTQWE"
     gene            complement(884..2266)
                     /locus_tag="PKI50_11735"
     CDS             complement(884..2266)
                     /locus_tag="PKI50_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNT04410.1"
                     /translation="MYKPVAEPFRIKMVESIRMISKEERVKALKEAGYNPFALKAEDI
                     YIDLLTDSGTGAMSDYQWSGMMLGDESYAGSRNFYNLLEAVKDIIGYPYCVPVHQGRG
                     AEKVLFSNLIKKKGQYVLGNMHFDTTMAHIELNGGRPLNFITEKAFNTTEPYEWKGNF
                     DLEKLEAFIKENDRENIAFILITITCNSSGGQPVSMENIKGVHALAKKYGIRLFIDAA
                     RFAENAYFIKRREKGYENKSITEIVREMFSYAEGFTMSAKKDAIVNIGGLIGIKEDEE
                     LFTAVKTTLIPYEGFVSYGGLAGRDMEALARGLREGVNEDYLHYRISQVEYLGYRLTE
                     GGVPIQLPTGGHAVFVDAKKMLPHIPYYQFPAQVLANALYIESGVRAVEIGSFLLGRD
                     PETGEHRESPLELMRLTIPRRVYTNSHMDYIADALIYLKDKVNTLKGLDFEYEPKVLR
                     HFTARLKPIE"
     gene            complement(2400..3614)
                     /locus_tag="PKI50_11740"
     CDS             complement(2400..3614)
                     /locus_tag="PKI50_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNT04411.1"
                     /translation="MYGLALEGGGVRGAFHMGAYKALLEEGYEFGGVTGTSIGALNGA
                     IIAQGDFEAGYKLWESMDTPLLFDIEEEQYRKLIDRKIDRELLLRLAARMGEILENKG
                     INTSKIRQVLESIINEEKLRKSNIDFGLVTVSITDLKPLELYKENIPEGKMIDYLMAS
                     ASFPGFKLNPIGDKYYIDGAFYDNCPINLLARKGYKDIIAVRTLGLGIVQHVRYQSVK
                     VTNIFPSEDLGSPLDFNRDLIIRNLQLGYYDTMKLLKRLKGRKYYIIPEDEEKVFEIF
                     RSLPDMLVRELGKLFNTKEMTVKRMLFERIIPEIADLLGLKAASEYQDILIGLLEYLA
                     EESGVERFRIYSVKEFMKEIKFQREGKSRMHVKRPVLTGRKSLNTRLAYILKRKSRLS
                     KAAKNIFDEISS"
     gene            3753..6722
                     /locus_tag="PKI50_11745"
     CDS             3753..6722
                     /locus_tag="PKI50_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT04412.1"
                     /translation="MKHLVSSLICIFLVLMSLFQINIYAENNDISITPSIIFEDSSES
                     YPIDGEQVILYTNKYGEFTRPFGAHTHEFVIVNNIVVQENSNGANGTYIPPNGCVISY
                     TGDNEDFRRKFSVGTGLTLTNIDIPVSPDMYFILEDMLIPIDQINTVRDANQIILYNA
                     AYNTSTKTNPWGMELTVEDGIITKIADMTNTEGLQGRNNSQIPTDGAVISIHAGSPYY
                     MQVREKAKPGININISADAKLYNASKIKYAAYNPRTLADNPAAWDSMKEKPYDSFRGP
                     DQLIIYDSSYGEFTGTNPYGYEITVNSEGIIIGTGGNDSKIPEGGYVLSGHGESMKWL
                     QKNALLGSTVTANHDKKEVTIILTPESYTSRALLNIKSAQDRLGLAKLQYLDIPYDDV
                     RKAIDVAADKLSKLNRQLKEGRYENLAGTVQEIQKVTDKAWFMTFESVKAENRAVWLR
                     PRDINIEDVKKSFDMLESININTIYLETYWNGYAIYPTGNDLMRQNPLFKGFDVLDSY
                     IKEAHSRGIELHAWVENFLVDLPIAEKKPEWMNVSRKGDTYYLENGITKYYFLNPALP
                     EVYDFLSSLYTELIEKYDIDGIQFDYLRYPNSHDYTNDFGYDTYTRQLFANFTGTDPI
                     SLMPGDSLWQEWCEFRQYIISSFACRIFSELKSIQPEIQISADVWPDYDETLSDIYQN
                     PKTWVRKDYINTLIPMSYYLHEKPVIEDLENTWSFSRGHSRITSGIATFNKVDTKVFI
                     KQIDAVRASNTSGIAIFEYESLFSSGYDYALKLGAFSTPSAVTGKNPEESINIILKDI
                     IRKLDDVYLKNNGINKNQRNKYKNLIEKLGAGFDEEQKNNIQEAIKAIYNDKNLNVEI
                     SKRIIADLNRAINIIDGHIANTRLMERLRIIAEKSDGVLKAAEISYSRVKLGWGSNAN
                     DSGIAGYILYRNGKEILRTSSYSINDNDLDFGGTYTYEVHGFDAVGNIVYRSNSLTVR
                     TKSSLLAVSSPPKNTIF"
BASE COUNT         1992 a         1558 c         1306 g         1991 t
ORIGIN      
        1 cttcccattg tgtaaatacg gcttgtgcaa agtttggatc gaaatggata cctcctacac
       61 aaagaaggtt gtgcacgcgt ttgccgtcac tttcaaacac atgggttaat gcgcctgcca
      121 gagcttcaca ggctgtacgg ttgttccagc tgtcctcctc gcttcctacc tcaatatcca
      181 ccataggcac cggataatgc aaaatcaatt ccggtgaact tccgcttacg gcagtacccg
      241 accaatgagt cgcctctgta accgtcatgt aatccgaaag ccccagcata acgcggttat
      301 gttccatcga aatcagtatg tttctcatat aagcagggtt agccggtcca tatactcccg
      361 cattcacatc tcctatagta tggactgtaa gcaccttagg cggtgcactc gccccctcgt
      421 gccaggtgac catgcccgaa aaatctgcat ccgcaaatct ctcgttcatc tccgccaagt
      481 atctcggata atcccagcaa acaggaactt ccgactgtgc gaaataatat tcattacctg
      541 ccccgtcacg atgcatcata acctcgcggt tatcaaatga cattcccgca ggttttaata
      601 agcctttatc attcaatatt tcccacacga cacgtgagac agtgcccggc cgcccttcgt
      661 ttgtgctgat ataataaacc gctttcttga cctctgacat attatgtctc ctttctgctg
      721 taccgttccg gaacggtaca atacaaggct gctgcaaaat gtatcatttc gcagcagccc
      781 aaacagctag tttcagccta atccttttgt agtggtctac aaggaaggat acattgactt
      841 caaggattga ttatggccct tgttttctcc ataattatcc tcgctattct attggcttca
      901 gccttgcagt aaagtgcctt aatactttag gctcatattc aaaatccagc ccctttaatg
      961 tattgacctt gtcttttaag tatataaggg catcagcgat ataatccata tggctgttcg
     1021 tatacactct tctgggtatt gtcagcctca ttagttcgag aggcgattct ctgtgttctc
     1081 ccgtctccgg atcccgccca agcaggaagg atcctatttc aactgccctt actcctgatt
     1141 cgatatacaa agcatttgcc agaacctggg ccgggaattg atagtacggt atatgcggca
     1201 gcattttctt tgcgtctacg aataccgcat ggcctcctgt tgggagttgt atcgggacgc
     1261 ctccttccgt aagcctgtaa ccgaggtatt caacctggga tatcctgtaa tgcaggtaat
     1321 cttcatttac accttccctg aggcctcttg ccagagcttc catgtctctt cctgcaagac
     1381 ccccgtatga tacaaatccc tcgtaaggga tcaaagtagt ttttacggcg gtgaataact
     1441 cttcatcttc ctttatgccg ataaggcctc cgatatttac tatggcatcc tttttggcgc
     1501 tcatggtaaa gccttcggca taagagaaca tttcccgaac tatctcagtg atactcttat
     1561 tttcatagcc cttttccctg cgctttataa aataagcgtt ctccgcaaac cttgcagcat
     1621 ctatgaacag ccttatgcca tattttttag cgagagcatg gacccctttt atgttctcca
     1681 tggatacggg ctggcctcct gagctgttgc aggttatggt tatcagaata aaagcgatgt
     1741 tttctctatc attttcctta ataaaagctt ccagcttttc aaggtcgaaa ttacctttcc
     1801 attcataagg ttccgttgta ttgaaggctt tttcagtaat aaaattcaaa ggccttccgc
     1861 cgttcagctc aatatgagcc atagtggtat caaaatgcat attcccaagt acgtattggc
     1921 cttttttctt tatgagattg ctgaacaata ctttttccgc tcccctgccc tgatgtaccg
     1981 gtacgcaata cggatagcca ataatatctt ttactgcttc cagaagatta tagaaattcc
     2041 tgctcccggc ataggattca tctcccaaca tcatgcctga ccactgatag tcgctcatag
     2101 ctcccgtccc actgtctgtc aacaggtcga tatatatgtc ttcggccttc aaggcaaagg
     2161 gattatatcc tgcctctttt aatgctttta ctctttcctc cttggaaatc attctgatgg
     2221 attctaccat tttgatcctg aaaggttccg ctactggttt atacatttca attccccctt
     2281 ttattataga ataatgatac tggatccttg ctgatgcaaa tcaggccgtc ttgtcccaat
     2341 aggatatgcg gaaaagtttc cttaaaaaag tactttctgc tcactctcct ttccgtctgc
     2401 tatgagctga tttcatcaaa tatatttttt gctgctttgc tcaacctgct ttttctttta
     2461 agaatatatg ccaatctcgt gttcaggctt tttctgcccg ttaataccgg ccttttgaca
     2521 tgcattctgc ttttaccttc cctttgaaat tttatttcct tcatgaactc tttcactgaa
     2581 tatatcctga atctttccac cccgctttct tcagcaaggt attccagcag gccaatgagt
     2641 atgtcctgat actccgaggc tgctttcaat cccaataagt cggcaatctc cggaattatt
     2701 ctttcaaaca gcattctttt tacagtcatt tccttagtat tgaacagttt tccaagttcc
     2761 ctaaccagca tatcaggcaa actgcggaaa atctcaaata ctttttcctc atcttcgggt
     2821 ataatgtaat acttccttcc tttaaggcgc ttcagcagct tcattgtatc ataataaccc
     2881 aactgaagat tgcgaattat cagatcccgg ttgaagtcca aggggcttcc caggtcctca
     2941 gaagggaata tatttgtgac cttcacgctt tgatatcgca catgctggac aatgcccaat
     3001 cccagggttc tgactgcgat tatatcctta tatccctttc tggccagcag attaatgggg
     3061 caattatcgt aaaaggctcc gtcaatatag tatttatccc cgatggggtt taatttgaat
     3121 ccgggaaaac ttgcacttgc cataaggtaa tcaatcatct ttccttcggg aatattctcc
     3181 ttatacagct ccaaaggctt taaatcagta atcgacaccg ttaccaggcc gaagtcaata
     3241 tttgatttcc ggagtttttc ttcatttata atgctttcaa gcacctggcg tattttgctg
     3301 gtatttatgc ccttattttc aagtatttcg cccattctgg cagccagcct taacagcaat
     3361 tccctgtcta tttttctgtc aatcagcttt ctgtattgct cttcttcaat atcaaacaat
     3421 aaaggagtat ccatactttc ccatagcttg tatcctgcct cgaaatcacc ctgggcaatt
     3481 atggctccgt tcagtgctcc tatggatgta cctgtcactc ctccgaactc gtatccttct
     3541 tccagcaagg ctttataagc acccatgtga aaagcgcccc tcacacctcc gccttcaagt
     3601 gcaagaccat acataaaaac tcctcccctt gaataatata tcattttgtg gcttgccaga
     3661 caagctattt taatatgctt cagttgcttc acatatacat gctctgctat aatttatatg
     3721 acataaaatg ttgaaaataa aggagcaatg atatgaagca tcttgtaagc agcctgatat
     3781 gtatattctt agttttaatg tctttatttc aaatcaatat ttatgccgaa aacaatgata
     3841 tcagcatcac tccttcgatt atattcgaag acagctctga atcatacccg attgatggag
     3901 aacaagtcat attgtatacc aacaaatatg gtgaatttac caggcctttc ggagcacata
     3961 cccatgagtt cgtcattgta aacaatattg ttgttcaaga aaacagcaac ggcgccaatg
     4021 gtacttatat tcctccgaac ggatgcgtaa tatcatatac cggcgacaat gaggatttta
     4081 gaagaaaatt ttccgtgggt acaggattaa cacttaccaa cattgatatc cccgtttctc
     4141 ccgatatgta ttttatactg gaggatatgc ttataccaat tgatcaaatc aatacagtac
     4201 gggatgccaa tcaaataata ttatataatg ctgcttacaa tacctcgaca aagacaaacc
     4261 cgtggggaat ggaactgacg gttgaagacg gcattatcac caaaattgca gatatgacaa
     4321 ataccgaagg gctgcagggc cggaataatt cgcagatacc gacggatggt gcggtaatat
     4381 caatacacgc aggaagccca tactacatgc aggtacggga aaaagcaaaa ccgggtatta
     4441 atattaatat atcagctgat gctaagctgt ataatgcaag taaaatcaaa tatgccgcat
     4501 acaacccaag aacattggca gataaccccg ccgcatggga cagtatgaaa gaaaagccat
     4561 atgacagctt cagagggccg gatcaattga taatctatga cagcagctac ggtgaattta
     4621 ccggtacaaa cccttatggc tatgagatta cggtaaacag tgagggaata ataattggca
     4681 caggaggcaa tgactcaaaa atacccgaag ggggatatgt actttcagga cacggtgaaa
     4741 gcatgaaatg gcttcaaaag aatgccttat taggatcgac ggttacagca aaccatgaca
     4801 aaaaagaagt aactataatc cttactccgg aatcatatac aagcagggct ttattgaata
     4861 ttaaatcggc acaggacagg cttggcctgg caaagcttca gtatctggac attccatatg
     4921 atgatgtgcg gaaagcaata gatgttgctg ccgacaagct gtcaaagctt aacaggcaac
     4981 tgaaggaagg ccggtatgaa aacttggccg gcaccgttca agagatacaa aaggttacgg
     5041 acaaggcatg gttcatgacc tttgagtctg taaaggcaga gaacagggca gtatggttaa
     5101 ggcccagaga cataaatatt gaagatgtaa aaaagagttt tgatatgctg gaatcaatca
     5161 acatcaatac catatatctt gaaacctact ggaacggata tgccatatat ccgacaggca
     5221 atgacttgat gaggcagaat cctttattca aaggatttga tgtcctggac tcatatatta
     5281 aagaagcgca ttcgagagga attgaacttc acgcatgggt ggagaacttt cttgtggatc
     5341 tgcccatagc tgagaaaaaa cctgagtgga tgaatgtaag cagaaagggc gatacttatt
     5401 atctggaaaa cggaataacc aaatattact ttttgaatcc cgccctgcct gaggtttacg
     5461 atttcctatc atcgctgtat acagaactaa tcgaaaaata cgatattgac ggtattcaat
     5521 ttgactactt gagatatcca aattcccacg attatacaaa tgacttcggc tatgatacat
     5581 ataccaggca gctgttcgct aattttaccg gtactgaccc aatttcactg atgccggggg
     5641 actcattatg gcaggaatgg tgtgaattca gacagtatat tattagttcc tttgcttgca
     5701 ggatattttc agaattaaaa tcgatacagc ctgaaattca aatatccgct gatgtatggc
     5761 cggactatga tgaaaccctc tccgacatat atcagaatcc aaagacatgg gttagaaagg
     5821 attacatcaa taccctgata cctatgtcct actatctgca tgaaaaacct gtcattgaag
     5881 atttagaaaa tacctggtcc ttctcaaggg gccattcccg gatcacttca ggaatagcaa
     5941 cgtttaacaa ggttgatacc aaagtgttta taaaacaaat tgacgctgta agggcttcaa
     6001 atacaagcgg cattgcaatc tttgaatacg agtcgctgtt cagcagcggc tatgattatg
     6061 cattgaagct cggagccttc agcaccccct cggcagttac gggtaaaaat ccggaagaat
     6121 cgataaatat aattttaaaa gacataataa gaaagcttga tgatgtatat ctgaagaata
     6181 atggtataaa caaaaatcag agaaacaaat acaaaaacct tattgagaag ctaggagcgg
     6241 gctttgatga agaacaaaaa aacaatatac aagaagcaat aaaggctata tataatgata
     6301 aaaatctcaa tgtggaaatc tcaaaacgaa tcattgctga cttgaaccgc gcaataaata
     6361 ttatcgacgg gcatattgca aatacaaggc ttatggaaag acttagaata attgccgaaa
     6421 aatccgacgg tgttttaaaa gccgctgaaa taagctattc aagagtgaag cttggatggg
     6481 gaagcaatgc aaatgactca ggcatagcag gttatatttt gtaccgcaat ggcaaagaaa
     6541 tcctcagaac ttcttcttat tccataaatg ataatgactt ggacttcggt gggacttaca
     6601 cctatgaggt acacggattt gatgcagtag gaaacattgt atacagaagc aacagtctga
     6661 ccgtcagaac aaagtcatcg cttcttgccg tcagcagccc tcctaagaat actatttttt
     6721 aagaacgtcc gtattaacaa gcgtcttcgg gactttgcca ttcatagcag ctataatatt
     6781 tcttgccgct atcataccca tgttaattct cgcttctata gtagcactcc caatgtgagg
     6841 cagcatc
//
LOCUS       DAOQUR010000091         6423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_13299_length_6423_cov_10.379711, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000091 DAOQUR010000000
VERSION     DAOQUR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13299_length_6423_cov_10.379711"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKI50_11750"
     CDS             complement(<1..749)
                     /locus_tag="PKI50_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNT04413.1"
                     /translation="MSSGLYSMNKREFDIAFDPKRVKPYGDTMNDGKVQLSFTLPIPA
                     DELAKEAAKLYAMKMGLEEPSVVHMADLGVGYSFFVVYGSCIHTIDITDIKVTKVESS
                     VMSMDEVDEYIRKNIGRKLVVIGASTGTDAHTVGIDAIMNMKGYAGHYGLERYEMIEA
                     YNLGSQVPNEEFIKKAVELNADALLVSQTVTQKNVHVQNMTNLIELLEAEGLRDRFIV
                     ACGGSRISHELAKELGFDAGFGAGTYAEDIAS"
     gene            complement(761..2308)
                     /locus_tag="PKI50_11755"
     CDS             complement(761..2308)
                     /locus_tag="PKI50_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNT04414.1"
                     /translation="MSSLKLDVRLIGSARESAMQIAGDVQEFIDIHTTVAVERTITRL
                     LGVDGINDVGTPLPNVVVENIKNAGGLGIGAAYFLVNAVLNTGLTTQEIAEKVSAGEL
                     DLTKLPAKPEKEIKDKAYELAAKMVERIRSNREQRDEYLNNIGEGKKPYLYVIVATGN
                     IYEDVVQAQAAAKQGADVIAVIRTTGQSLLDYVPYGATTEGFGGTYATQENFRIMRKA
                     LDETSREVGRYIRLCNYCSGLCMPEIAAMGAMERLDVMLNDALYGILFRDINMQRTLV
                     DQNFSRIINGFAGVIINTGEDNYLTTADAVEEAHTVLASQFINEQFALKAGIPEEQMG
                     LGHAFEMDPGIENGFLMELAQAQMAREIFPKAPLKYMPPTKYMTGNIFKGHLQDALFN
                     IISIWTNQGIQLLGMMTEALHTPFMADRALSIENARYIFNNMRSIGDEVEFKEGGMIQ
                     SRAAEVLEKAAALLKEIAEEGLFATLEQGKFAGVKRSRTGGKGLDGVTEKDAKHINPF
                     IELMLNR"
     gene            complement(2327..3922)
                     /locus_tag="PKI50_11760"
     CDS             complement(2327..3922)
                     /locus_tag="PKI50_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT04415.1"
                     /translation="MLMDENTKAIFEFDRVLEELHPMTPFGQKLKNNVKAYEALDKEL
                     LLEELDRVAVLKGLINSQRPVFVEIRSQMRLIKDIRRSVERCTAGGVLNVVEFFELKN
                     FAYIAKAISKCQKALHWAMPEKYRVKELQWVEAILDPEKTGMKTFYIYDNYSEALAEI
                     RSRKAASLHKLDVLKKEAIKRAEAELGIPVRASGEITVSKTQTNLIKKFDECNMLQPA
                     GETYINVTYRVKPGAEMLELMKDIEEMKGEEAMEEALILEKLSAQISVRGSEILEVMD
                     AVAEFDLVIAKAYMANGYNGVKPVICDDEKLVIVKGRHPLVETSLRRKGKPFTPVSIS
                     LEPGAALITGANMGGKTVSLKMVGLLAAMAQYGFLVPAEYMEMRMNEFIYISAGDEQS
                     IDMGLSTFGAEIRSVKEALMKSEEKGLILIDELARGTNPHEGYAISKAIINYLKDKPG
                     MALITTHFDGLAGEGIRHLQVKGLRDIDFKSIKDPDAVSEYMDYTLIEVEGESKVPQD
                     AINISRLMGIPEEILRQAEEIMK"
     gene            complement(3916..4956)
                     /locus_tag="PKI50_11765"
     CDS             complement(3916..4956)
                     /locus_tag="PKI50_11765"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HNT04416.1"
                     /translation="MNQLIDLIYPQNKIVSIVGTSKNAGKTVTLNEIINQAHEKGIKL
                     GLISTGRDGERRDVLTNTEKPPIFVRKGTVLTTAESAIKGEHAGLEIFRVTDYNTPLG
                     RVIIGRATEDEYAEISGPHSSLTIKKMCIEMQELGAELILIDGSLDRRASAAPYVSDG
                     TVLATGAALARNIGGVVEKTMHLINTYSIPAAEDEHTRDIAYSAVDMGKTALITFDGS
                     VRYIDTVTSLRSGDIISEQLGEDTDSIVLSGSATVDTIKDILLNMKSSLKIIVKDPTR
                     IFIPASDFAILQKMGMKLRVLENVNIIAITVNPYSPEGYCFEPCELLEAMRNAVPHIP
                     VFDVIQGEKLIC"
     assembly_gap    5036..5135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5147..6406)
                     /gene="ablA"
                     /locus_tag="PKI50_11770"
     CDS             complement(5147..6406)
                     /gene="ablA"
                     /locus_tag="PKI50_11770"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNT04417.1"
                     /translation="MRNYKDIPLWKDVSPEQWNDWKWQVSNRITTAEQLKQVIELTPE
                     EEAGVNECLKTLRGAITPYYASLMDPKDPHCPIRKQAVPTSKELHKADADMLDPLHED
                     EDSPVPGLTHRYPDRALLLITDQCSMYCRHCTRRRFAGQHDEQMPMERIEKAIEYIRN
                     TPVIRDVLLSGGDPLLVNDDVLEYIIKKLREIDHVEIVRLGSRTPVVMPQRITDDLVN
                     MLKKYHPVWLNTHFNHPKEITPESSAACNKLADAGIVLGNQSVLLRGVNDCVHIMRDL
                     VHGLVKIRVRPYYIYQCDLSFGIEHFRTTVSKGIEIIESLRGHTSGFCVPTFVVDAPG
                     GGGKTPVMPQYVISQSKSKVILRNFEGVITTYTEPEYIEEPCTCDVCTGKKKDKLSGV
                     AGLLEGNAMNLEPTEMLRKERAKARHN"
BASE COUNT         1401 a         1716 c         1255 g         1951 t
ORIGIN      
        1 gaggcaatat cctccgcata ggttcctgct ccgaagcctg catcgaaccc aagctccttt
       61 gcaagctcat ggcttatcct cgaccccccg caggctacta tgaacctgtc cctgagccct
      121 tccgcttcaa gcagctcaat caggttggtc atgttctgca catgtacgtt tttctgggtc
      181 actgtctgag atacaagaag tgcatctgca ttaagctcca cagctttttt tatgaattcc
      241 tcattaggca cctggctccc aagattatag gcctctatca tttcatacct ttcaaggccg
      301 tagtggcccg cataaccctt catgttcatt attgcatcta ttcctacggt atgagcatct
      361 gtacctgtac tggcgcctat aaccacaagc tttcttccaa tattcttcct tatatattca
      421 tccacctcgt ccatactcat taccgagctt tcaaccttgg ttacctttat atcggttata
      481 tcgattgtat gaatacagct tccgtagact acaaagaagg agtagccgac tcccaggtcc
      541 gccatatgca ctacactggg ctcttcaagc cccatcttca ttgcataaag cttggcagcc
      601 tcttttgcca gttcatccgc cggaatcggc agtgtgaagc tcagctgcac cttgccgtcg
      661 ttcattgtat ctccgtaagg cttcactctc ttggggtcaa aggctatatc gaattctctc
      721 ttattcatgg aatacaaacc ggaactcatg aaagcacctc ctatctattc aacatcaatt
      781 ctataaatgg gtttatatgt tttgcatcct tctccgttac gccgtccagt ccttttcctc
      841 ctgtcctgct tctcttcacc ccggcgaatt tgccctgctc aagggttgca aaaagccctt
      901 cttcggcaat ttccttcaga agcgcagcag ctttttcaag cacttcggcg gctctggact
      961 gaatcattcc gccttcttta aattccactt catctccgat actcctcata ttgttgaata
     1021 tatatctcgc attctctatg gagagggccc tatcggccat aaaaggagta tgcaaggctt
     1081 ccgtcatcat tccgagaagc tggatgccct gattcgtcca tattgatata atgttaaaaa
     1141 gcgcatcctg caaatgtccc ttgaatatgt tgcctgtcat atacttggta ggaggcatgt
     1201 attttaaagg agccttgggg aatatttccc ttgccatctg ggcctgggca agctccatga
     1261 ggaatccgtt ctcgataccc ggatccattt caaaggcgtg tcccaaaccc atctgctctt
     1321 caggtattcc tgccttaagg gcaaactgtt cgtttataaa ctgggaggcc aataccgtat
     1381 gtgcttcttc aacggcatcg gctgttgtaa gatagttgtc ttctccggta ttgattatta
     1441 caccggcaaa tccgtttatt atccttgaaa agttctgatc caccaaggtt ctctgcatgt
     1501 ttatgtctct gaaaagtatc ccgtataatg cgtcgttcag cattacatca agcctttcca
     1561 tggcacccat tgcggcaatt tccggcatgc agagcccgga gcaatagttg cagagcctta
     1621 tataccttcc tacctcccgg cttgtttcat caagagcctt tctcattatc ctgaaattct
     1681 cctgagtggc ataggttcct ccgaagcctt cggtagtggc tccgtagggc acataatcca
     1741 gtaggctctg gcctgttgtc ctgataactg ctataacatc agccccctgc tttgcggcag
     1801 cctgtgcctg cactacgtct tcatatatgt taccggtagc gacaatcaca taaagatacg
     1861 gctttttgcc ttccccgata ttgttcagat attcatcccg ctgttccctg ttgcttctta
     1921 tcctttcaac catttttgcc gccagttcgt aagccttgtc tttgatttct ttctccggct
     1981 ttgcaggcag ctttgtcaaa tccagctctc ccgccgatac cttttcggca atttcctgag
     2041 ttgtcagccc cgtattcaag accgcattca ccagaaaata tgcggctcct atgccaaggc
     2101 ctccggcatt tttgatattt tctactacaa cattgggaag gggggttccc acatcattta
     2161 tgccgtcaac tcccaaaagt cttgtaattg ttctttccac tgccactgta gtatgaatat
     2221 caatgaattc ctgcacatct ccggctattt gcatcgctga ttctcttgca ctgcctatca
     2281 atctaacgtc aagcttcaga ctgctcatga cacacctcca tgactattat ttcattattt
     2341 cttccgcctg gcgcagtatc tcttccggta ttcccatcag gcgggatatg tttattgcat
     2401 cttgaggaac cttggattcc ccttccactt ctatgagagt gtaatccatg tattcggata
     2461 ctgcatcagg gtcctttatg ctcttaaaat cgatatctct aagccccttg acctgcaaat
     2521 gcctgatgcc ctcccctgca agcccgtcaa aatgcgtagt aattaaagcc atacccggtt
     2581 tatctttaag ataattaata attgctttgg atattgcata gccctcatgg ggatttgttc
     2641 ctctggccag ctcatctatc aatatcagcc ctttttcctc cgacttcatc agcgcttcct
     2701 taacagagcg gatttccgcg ccgaaggtac tgaggcccat atctatcgac tgttcatctc
     2761 ctgcggaaat gtatatgaat tcattcatcc tcatctccat gtattccgca ggtacgagga
     2821 atccatactg agccatagcc gcaagcaagc caaccatctt gagggatact gttttgcctc
     2881 ccatattggc ccccgttata agcgccgctc ccggttccag gctgatgctg acaggggtaa
     2941 agggcttgcc ctttcttctg aggcttgttt ctaccagggg atgccttccc ttgactataa
     3001 caagcttttc atcatcgcaa atcacaggct ttactccatt atagccgttg gccatgtatg
     3061 cctttgctat gaccaggtcg aattccgcca cagcatccat tacctccagt atctcgctgc
     3121 ccctgacgga tatctgcgct gaaagctttt ccaatatcag cgcctcttcc atggcctctt
     3181 cgcccttcat ttcttctatg tccttcataa gctccagcat ttctgcaccg ggttttaccc
     3241 tgtaagttac atttatgtat gtttctcctg ccggctgaag catattgcac tcatcaaact
     3301 tcttaatcag attggtctgg gtcttgctga cggttatttc tccgctggct ctcacaggta
     3361 tgccaagctc cgcttccgcc ctctttattg cttctttttt taaaacatcc agtttgtgca
     3421 ggctggctgc tttcctgctc cttatttctg ccagagcctc cgagtaattg tcatagatat
     3481 agaaggtctt catccccgtt ttttccggat caagtatggc ttccacccat tgaagctcct
     3541 tcaccctata tttctcgggc atggcccaat gaagcgcttt ctgacatttt gatatggcct
     3601 ttgcaatata tgcaaaattc ttcagctcaa aaaactccac aacattcagt acgcctccgg
     3661 ctgtgcacct ttccacagac cttctgatat cctttatcag cctcatttgt gaccttattt
     3721 cgacaaatac gggtctctgc gaatttataa gccctttcag cacagccacc ctgtccagct
     3781 cttccaacag cagctcctta tccaaggctt cataagcctt gacattgttc tttagcttct
     3841 gtccgaaggg agtcataggg tgcagctcct ctaggaccct gtcaaactca aatattgctt
     3901 ttgtattttc atccatcagc atatcaactt ttctccttga atcacatcaa aaaccggtat
     3961 gtggggtact gcattcctca tagcttcaag cagctcgcaa ggttcaaagc agtagccctc
     4021 cggcgaataa ggatttaccg ttattgctat gatatttaca ttttccagta ccctaagctt
     4081 catacccatt ttttgaagta tggcaaaatc cgaagcaggt atgaatatcc tggtagggtc
     4141 cttcactatt atcttgaggc tgcttttcat gttcagcagt atatccttta tggtgtcaac
     4201 cgttgcggaa ccgctcagca ctatactgtc ggtatcctcc ccaagctgct ccgaaattat
     4261 atctccggac cgaagtgagg taacagtatc gatatatctt acacttccgt caaaggtaat
     4321 caaggctgtt tttcccatgt caactgcgct gtatgcaata tctctggtat gctcatcctc
     4381 tgctgccggg attgaatagg tattaataag atgcattgtc ttttccacca ctccgcctat
     4441 attccgggca agtgcggcac ccgtcgccag aaccgtcccg tccgaaacat aaggggctgc
     4501 cgaggcccgc ctgtccaggg agccgtcgat caatatcagc tccgctccca attcctgcat
     4561 ctcaatgcac attttcttta tggtcaggga tgagtgaggc ccggatattt cagcatactc
     4621 gtcctcggtg gcacggccga tgatgactct ccccaaaggg gtattgtaat cggtcactct
     4681 gaatatctcc aagcccgcat gttcaccttt tatcgcgctt tccgccgtgg tcagaacagt
     4741 gcctttcctg acaaaaatgg gaggcttttc ggtattggta agcacatccc tccgctctcc
     4801 gtctctgccc gtagaaataa ggcccagctt tattcccttt tcgtgggcct gattgattat
     4861 ttcatttaag gtcacagtct tgccggcatt cttggaggta cctactattg atactatttt
     4921 gttttgcgga taaataaggt caataagctg attcatcaag atcctcctgc ttctggctcc
     4981 tttgagatac gcgggatatg gggacatggg acaggagaaa cgtccccgtg tcccannnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgt gtcccattaa ttgtgccggg
     5161 cttttgccct ttcttttcgg agcatttccg tcggctccag gttcattgcg tttccttcca
     5221 gaagcccggc tacgcctgac agcttatctt tcttcttccc ggtgcatacg tcacaggtac
     5281 atggttcttc tatatattca ggctcagtgt aagtagtgat aacgccctca aagtttctga
     5341 gtatcacctt gctcttgctc tgggaaatca catactgcgg cattaccgga gtctttccgc
     5401 cccctcccgg tgcatcaaca acaaaagttg gtacgcagaa acccgatgta tgccccctga
     5461 gactttcaat tatttcaata cccttggata ctgtagttct gaagtgttct atcccgaagg
     5521 aaaggtcgca ttgataaata tagtatggcc ttacgcggat cttcaccagt ccgtggacca
     5581 aatctctcat aatatgaaca caatcgttta ctcctcttaa gagcaccgac tggttgccga
     5641 ggactattcc ggcatcggca agcttattgc atgcggctga ggattccgga gtaatctcct
     5701 tgggatggtt gaaatgagta ttaagccata cgggatgata tttcttaagc atgttaacaa
     5761 gatcgtcggt tattctctgg ggcattacta cgggagttct tgatccgagt ctgacaatct
     5821 caacatgatc tatttccctg agctttttga ttatgtattc cagtacgtcg tcattgacaa
     5881 gcagcggatc tcctcctgag agaagcacat cccttataac gggagtattt ctgatgtatt
     5941 cgatggcctt ttcgattctt tccataggca tctgctcgtc atgctgccct gcaaatcttc
     6001 tccttgtaca gtgcctgcag tacattgaac actggtctgt gataagcagc agtgccctgt
     6061 cgggatacct gtgggtcagt cccggtaccg gtgagtcttc atcctcatga agaggatcca
     6121 gcatgtctgc atctgctttg tgaagctcct tggaagtagg gacggcctgt tttctgatag
     6181 ggcaatgggg atccttcgga tccataaggg atgcataata tggagttatt gcccccctta
     6241 aggtctttaa gcattcgttc actcccgctt cttcttcggg agtaagttca attacctgct
     6301 taagctgttc tgctgtggtt atcctgttgg atacctgcca tttccaatcg ttccactgct
     6361 cgggagaaac atccttccat aacggaatat ccttgtaatt tctcaatgcg ctaacctcct
     6421 taa
//
LOCUS       DAOQUR010000092         6021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_14522_length_6021_cov_10.139122, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000092 DAOQUR010000000
VERSION     DAOQUR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14522_length_6021_cov_10.139122"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKI50_11775"
     CDS             <1..419
                     /locus_tag="PKI50_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNT04418.1"
                     /translation="MFAEFEKIIHEKAESGEALTAELLCSVYRDLNILYYGPDIVADG
                     LIDMEWARIPHFYSSFYVYKYATGFSAAMSISQQILKEGQPAVDCYLKFLKSGGSDYP
                     IELLKIANVDMSTKEPVDNALKLFGSLLDQMEELTS"
     gene            793..1428
                     /locus_tag="PKI50_11780"
     CDS             793..1428
                     /locus_tag="PKI50_11780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNT04419.1"
                     /translation="MVKIRNEEETDYKRVEEITRKAFWNLYIPGCIEHYLVHVMRSHK
                     DFLPELDLVIEVDNQIIGNIMYTKAKLVDESGEEKEILTFGPTCIMPEYQRMGYGKML
                     MEHSFEQAVALGYDVIVIFGNPNNYVSRGFKSCKKYNICLENGTYPAAMMVKELKPKA
                     LDGRKWVYYDSPVMMIDEEEAGRFDESLEKMEKKFQPSQEEFYIHSHSIIQ"
     gene            complement(1463..2701)
                     /locus_tag="PKI50_11785"
     CDS             complement(1463..2701)
                     /locus_tag="PKI50_11785"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNT04420.1"
                     /translation="MKTVPFSKSELEHIASTFHTPFYIYDETAIRNNVRRLKNAFQWN
                     PGFKEYFAVKSTPTPGILKLFKQEGCGAECASLTELMLAERCGFEGRDIMFTSNVTTV
                     EEYQYASRLGAIINLDDAAHILILEACTGIPETICFRLNFGDQLKYGESVLIDFTNSK
                     FGSAKPQLEAAYPAVRNLGAKHFGIHAQFGCHKRNPEFFGINARKVFAFAVELFEKTG
                     IAIDFVNLAGGIGIPYHAEDEPADIEAISLQIKAAYDETIGKSTLPPVSLYTELGIFM
                     TGPYGYFVSRVLHVKQAYKTFIGLDASTNSFMSPLRYNNYHHITIMGKESAEPVQHYE
                     ITGALCEDRDRFATDRLLPKITEGDLLVFHDAGAYGYSHGNNFNGRLRPAELLLQCNG
                     DVSLIRRHETPDDYFSTMIF"
     gene            2810..4126
                     /locus_tag="PKI50_11790"
     CDS             2810..4126
                     /locus_tag="PKI50_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT04421.1"
                     /translation="MYKYRNIADLLKKEIYSGNYKPGSKLPSIQKLSKETGLNSDTIV
                     KAYKELVQEHIAYAVPKSGFYVAKNKLGTGGSIIDMKNVYLPEGINPYKDFQLCIEQA
                     MAIYGQRLFEYGSPKGLTELIQVLPKHLLGLQVFAKAENICITNGSQQALYILSAMQF
                     PQGGTRVLVEQPTYSLMLRILENARIPALGIRRTRDGIDLGQLKKLFQQGDIKFFYMI
                     PRYHNPTGFSYGNTQKREILKLAEQYGVFIVEDDYLADLEVDQKAETLHTLGNKDRIV
                     YIRSFSKTLLPGLRLGMAVLPESLQKHFLDFKSSMDLNTSILTQAALEIFLRSSMYRF
                     HGKRTKEHYKNKMSRLQEYCKEPQGCGTWHIPQTGLYAYLETGRISSQLLERSLLDSK
                     VLVSSTSQSYLNGFEHPEGLRLCVCNAEDQKIEKAVRIIEAKLAEF"
     gene            4160..4531
                     /locus_tag="PKI50_11795"
     CDS             4160..4531
                     /locus_tag="PKI50_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04422.1"
                     /translation="MDDFTKKDMEEALRAIASMIGRTEKAGEKFAQGTSQHTLQKNRL
                     EALNTASLLISKELADSNAMKHYTKEDLEKALAPISSLINKSEKARKKLAEGTWQHTM
                     LSNNLKALYIASRLFTKALSG"
     gene            4546..5295
                     /gene="arr"
                     /locus_tag="PKI50_11800"
     CDS             4546..5295
                     /gene="arr"
                     /locus_tag="PKI50_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HNT04423.1"
                     /translation="MNIRFDPNNVVIKLCMSGMNLEDGGNIEDAATMFHKAWHESTDD
                     YERFIAAYFLARRQKNTADKLKWMETSLQCALNINDDNVKSAYPTLYLNIAECYAELC
                     DSDNAKRNYELSNSYRGAPSDEGPFYHGTKADMQVGDLLTPGGNSNYKPELKMNHIYF
                     TANANGAGLAAALAKGEGRERVYIIEPTGEFENDPNVTDKKFPGNLTRSYRSQEPLRI
                     IGEETEWVKLTTAERSEWRENLAKNKGEIIN"
     gene            5463..>6021
                     /locus_tag="PKI50_11805"
     CDS             5463..>6021
                     /locus_tag="PKI50_11805"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HNT04424.1"
                     /translation="MIDIIVDEKDLSFNRLEKEIYAFGCKVACEVLKNVLETMDKKLA
                     KERDKAKYRHKGSRKTTVKTLMGEVAFSRAVYKSIGESSKEKYIYLLDQLLGFDAIGL
                     ISTNLAERIVENASIASYRNAADNITELSGQSISHSGVWNVVQALGQKIREEETELAK
                     AAEKNQLRGEKEVPVLFEEADGVYIN"
BASE COUNT         1923 a         1076 c         1451 g         1571 t
ORIGIN      
        1 ccatgtttgc tgaatttgaa aagattatcc atgaaaaggc cgagtccgga gaagcattga
       61 ccgcagagct gctgtgctcc gtatacaggg atttgaatat actttattat gggccggata
      121 ttgttgcgga cggccttatt gacatggagt gggcaagaat tccgcatttt tacagcagct
      181 tctatgtata taaatatgct actggctttt cggcagccat gtccatatca cagcagatac
      241 tgaaggaagg tcagcctgcg gtggactgct atctgaagtt cctgaaaagc ggcggttcgg
      301 attatcccat agagcttttg aagattgcca atgtggacat gtcgacgaaa gagccggtgg
      361 acaatgccct taagctgttc ggcagccttc ttgaccagat ggaggaactg acttcttaga
      421 ttgaagcaag atagcccata tatttttaca acctttatgc tttacttgct taatgatgtt
      481 gggaactgct tcatcctctg atcggggctt ttccttgaca gtgatatgac acattgagct
      541 ttatatgaag tatcaaagtg gagtttctgt gaaagaatta gaaaatatct attatttgtc
      601 aggtaaaact atctataaaa ttattgctga tattaaaacg aataattatt aaaacggtgc
      661 tatgagccaa aacctcatgg ccctttttat ttctgaaata tttaagagaa ttctttgggt
      721 gattgatatg atatacttaa ggcacaaaaa tttaatatta aataaattat cgggggttta
      781 taaatgaaca aaatggttaa gattaggaat gaagaagaaa cagattataa gagagtagaa
      841 gaaatcacaa ggaaagcctt ttggaattta tatattccgg ggtgcattga acactattta
      901 gttcatgtta tgcgatccca caaagacttt ctgccggagt tggatctggt gattgaagtt
      961 gataatcaaa tcatcgggaa tatcatgtac acaaaagcaa aactggttga tgagtctggg
     1021 gaggaaaaag aaatccttac cttcggtccg acttgcatta tgccggaata ccaaagaatg
     1081 ggttacggga aaatgcttat ggagcattct tttgaacaag cggttgcact tggctatgat
     1141 gtgattgtta tatttggaaa cccaaacaat tatgtaagcc gtggctttaa gagctgcaaa
     1201 aagtacaata tttgtcttga gaatggaact tacccagcag caatgatggt aaaagaactg
     1261 aagccgaaag ctctggatgg gaggaaatgg gtttactatg acagccctgt gatgatgata
     1321 gacgaagaag aagccgggcg ctttgatgag agtttggaaa aaatggagaa gaaattccag
     1381 ccaagtcagg aagaattcta tattcacagc cattctatta ttcagtgagt ttccttcagc
     1441 tcaggtgcca ggcatcactt ggctaaaaaa tcatagtcga aaaatagtcg tcgggggttt
     1501 catgccgcct gatgaggctg acatcgccat tacattgaag aagaagctct gcaggccgca
     1561 gccttccgtt gaagttattg ccgtggctgt agccataggc acctgcgtca tgaaacacca
     1621 gcaggtcacc ttcagttatt ttggggagaa gcctgtcggt tgcgaagcga tcccggtcct
     1681 cgcaaagcgc tccggtaatt tcatagtgct gaacaggctc tgccgattcc tttcccatga
     1741 tggtgatatg atggtagttg ttgtatctta acgggctcat aaagctgttg gtggaagcat
     1801 ccaagccaat aaaagtttta taggcttgct tcacatgaag gactcttgaa acaaaatacc
     1861 cgtatggccc ggtcatgaaa atccccagct cggtgtaaag agaaacagga gggagagtac
     1921 ttttgccgat ggtttcgtca taagctgcct ttatttgcaa gctgattgct tcaatgtctg
     1981 caggctcatc ctcagcatgg taagggatgc cgatgcctcc ggccagattt acaaaatcaa
     2041 tggctatgcc tgtcttctca aacaactcca cagcaaaggc aaataccttg cgggcattta
     2101 tgccaaaaaa ctcaggattt cgcttatgac atccgaattg tgcatgaata ccaaagtgct
     2161 tcgctcctaa attgcgcact gcggggtagg cagcttccag ctgaggcttt gcggagccga
     2221 acttgctgtt tgtaaaatcg ataaggactg actcaccata ttttagctga tcaccaaaat
     2281 tcagcctgaa acagatggtt tccgggatac cggtgcaagc ctccaaaatg agaatatgtg
     2341 ctgcatcatc caggttaata atggcgccaa gccgggaggc atactgatac tcttcaacag
     2401 ttgtgacatt tgaggtgaac atgatgtcac ggccttcaaa gccgcaacgc tctgcaagca
     2461 tcagctcagt cagggaagcg cattcggcgc cgcagccttc ctgcttaaaa agctttaaaa
     2521 ttccgggtgt gggtgtggat ttgactgcga aatattcctt gaaacccgga ttccactgaa
     2581 aggcattctt cagtcggcga acattgtttc taatggcagt ttcatcatat atatagaaag
     2641 gcgtatgaaa agttgaggca atgtgttcca gttctgattt ggaaaaggga acagttttca
     2701 tcttttatta ctcctttttt tatgtgatat aataattata tataaaatac cgatataaaa
     2761 gccagtatag aaatacatga gtataccggt atagatgggt ggccgtatta tgtataaata
     2821 caggaatata gcggatttgc tcaagaagga gatatattca ggaaattaca agcccggttc
     2881 aaagcttccc tcgattcaaa agctgtcaaa ggagacgggc ctcaacagcg atacgatagt
     2941 aaaggcctat aaggagctgg tacaggagca catcgcatat gctgtcccta aaagcggctt
     3001 ttatgtcgca aaaaataagc ttggcaccgg tggcagcatc attgacatga aaaatgtata
     3061 cttgccggag ggaatcaatc cgtataaaga ctttcagctt tgcattgaac aggcgatggc
     3121 gatttatggg caaaggctat ttgaatatgg gtcacccaag gggttaactg aactgattca
     3181 agtactgccg aagcatctgc ttggtcttca ggtttttgcg aaggctgaga acatatgcat
     3241 tacaaatggc tcacaacagg ctctgtatat tctgagtgca atgcagtttc cgcaaggcgg
     3301 taccagggtg cttgttgaac agcctactta ttcattgatg ctgcgaatat tggaaaacgc
     3361 aagaatacct gctttaggaa tccggaggac ccgggacgga attgatttgg ggcagctgaa
     3421 aaagctgttt cagcaggggg atatcaagtt tttttacatg attccgagat atcacaaccc
     3481 aacagggttc agttacggca acacgcagaa aagggagatt ctgaagcttg cagagcaata
     3541 tggcgtgttt attgtagaag atgattatct ggcggacctt gaggtggatc agaaggcaga
     3601 gacgctgcac actttgggaa ataaggaccg aatcgtctac ataagaagct tttcaaagac
     3661 attattgccg ggtttgaggc ttggaatggc agtccttccg gagtctttac aaaaacattt
     3721 cctggatttc aaaagcagta tggatctgaa tacttccata ctcacccaag cggcattgga
     3781 gatatttttg agaagcagca tgtatcgttt tcatgggaag cgtacgaagg agcattataa
     3841 aaacaagatg agcaggctgc aggagtattg caaagagccg caaggctgcg gcacctggca
     3901 tatacctcag acaggactct atgcttatct ggaaacagga agaatttctt cgcagttact
     3961 tgaacgaagc cttctggaca gcaaggtttt ggtcagcagt accagccaaa gctatttaaa
     4021 cggctttgaa catccggaag ggttgaggct gtgtgtatgc aatgcggaag accagaaaat
     4081 agaaaaggcg gtacggatta ttgaagcaaa gcttgcggag ttttgagaca agtaatattt
     4141 gaagagatga ggtttagtaa tggacgattt cacaaagaaa gatatggaag aagcactccg
     4201 agccatagcc tcaatgattg gcaggaccga gaaagcagga gagaaatttg cacagggtac
     4261 ttctcagcat acattgcaga agaacaggct tgaagcgctg aatacagctt cattattgat
     4321 atcaaaagaa ctggcggata gtaatgcaat gaaacattat accaaggaag atttagaaaa
     4381 agcgcttgcg ccaatatctt ccctgattaa caaatccgag aaagcacgga aaaaactagc
     4441 ggagggcacc tggcagcata ctatgctgag taataatctt aaagcacttt atatagcttc
     4501 acggcttttt acgaaagcat tgagcgggta gggaggcaag atcagatgaa tataagattt
     4561 gatccaaata acgtcgttat taagctctgt atgagcggga tgaatttaga agatggcgga
     4621 aacattgaag acgcagccac aatgtttcat aaagcatggc atgaatcaac agacgactat
     4681 gaaagattta ttgcagctta ttttttagct cgccgacaaa agaatactgc ggacaaattg
     4741 aaatggatgg aaacatcttt acagtgtgca ctaaatataa atgatgataa tgttaagagt
     4801 gcatatccaa cgttatattt aaacattgcc gaatgttatg cggagttgtg tgattctgat
     4861 aatgcaaaaa gaaattatga attgtcaaat tcatataggg gtgcgccttc tgatgaagga
     4921 ccattttatc atgggacaaa ggcagatatg caggttggtg atttactgac accgggcgga
     4981 aattcaaatt acaaacctga gcttaaaatg aaccacattt atttcactgc aaatgccaat
     5041 ggcgcaggac ttgcagcagc attagcaaaa ggagaaggaa gagaacgcgt ttatataata
     5101 gaaccaacag gtgaatttga gaacgatcca aatgttactg acaaaaaatt tcccggtaac
     5161 ttgacacgtt cttatcgctc tcaagagcct ttaagaatta tcggtgaaga aacagagtgg
     5221 gtgaaactga caactgcgga gcgaagtgaa tggcgcgaaa atttagctaa aaataagggc
     5281 gaaattatta actgaacatg ggtgcggctc gcgtcgtaaa tagcaaaatt ttcatcttag
     5341 agtataattt taattggcaa aataaatgac aaaataaagg gaagagacaa aaacctcttc
     5401 ccgtcataca aatcaaactg tagaatgtaa ttgtcaagaa aacatcgaca ggagtgattt
     5461 gtatgattga tataattgta gatgaaaagg atttatcatt caataggtta gagaaggaaa
     5521 tttatgcttt tggttgcaag gtggcctgtg aagtactcaa aaatgtatta gaaacgatgg
     5581 ataagaagct tgcgaaagaa agggataaag caaagtatag acataaaggg agtaggaaaa
     5641 caacagtgaa gacgctgatg ggggaagtag ctttttcccg tgcagtatat aaaagtattg
     5701 gtgagtcaag caaggaaaaa tatatatact tactggatca gcttttgggg tttgacgcaa
     5761 taggactgat atcaacaaac ctggcagaga gaatagttga gaatgcaagc atagcatcct
     5821 accgtaatgc tgccgataac ataacagagc taagcggaca aagtatcagc catagtggtg
     5881 tatggaatgt agttcaggct ttagggcaaa agataaggga agaagaaaca gaactggcta
     5941 aagcagcaga gaagaatcag ctgcgagggg aaaaagaggt acctgttctt tttgaagaag
     6001 ctgacggggt gtatataaac a
//
LOCUS       DAOQUR010000093         5758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_15374_length_5758_cov_12.456602, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000093 DAOQUR010000000
VERSION     DAOQUR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15374_length_5758_cov_12.456602"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PKI50_11810"
     CDS             <1..829
                     /locus_tag="PKI50_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNT04425.1"
                     /translation="YGGSSLEKEIKKLMQISAIITSILLITALISFLYVLKIYPASNR
                     RIIVYMIAGVSVSLLLIWGVSLELSRAYRTGKAKGFSIYVLEAGIGILLPLYIYLTGL
                     FSGDKDDIRRLYININNIAVRHNLQKNRPSKILMLLPGCMQDKDCSCKITDDMRNCRR
                     CGGCRIGEAADLVEKCAVRAIVVKGGTAARNTAKEFKPDFILAVACERELLSGMADVG
                     RTPVMGVINQRPNGYCTNTTVDMLQLKEILAELEEKGPELRCTKQGVKRDVKVQKNY"
     gene            947..2989
                     /locus_tag="PKI50_11815"
     CDS             947..2989
                     /locus_tag="PKI50_11815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT04426.1"
                     /translation="MEDNKSYITKSLLLWGILLVISIFLGYSIYNAFSGRIYANVFKS
                     MNNNLRFAESSYGVFMNQMKMGMLQASVEKNIKEMITEKDSAALEDLLKKWGEHRDYV
                     DEWYVVGEDGNIISSAGKGKDLLIKASGKTKIQEIVGEALSSGTVITGTELIPIGSRG
                     EFTLVQYVIVPVMENDRGVIGGIVTLMNLDGDDNITGKIMSDTGMHSIISAKDRIIAS
                     SLESELYRLNKGSSLPEDIRKEVLEKDTPFVKRLEIFRGSREDKDSYFGAFRAIKDIE
                     GSIIGVQGIIHNDPLAETTLGQIRDFTVIAVILLAAVFSIIIWSYINAQKVLLKEKQF
                     SNRLSSIKRFSDLVRQASNEDEVYEILFDVLKKKSGITQVVVAKKEYNDKCLTLYKAM
                     DMEKIQSIKNTMTVEENCWAVKSGKEFINNGNNNDFDCKDFYSESASYICLPIILGGI
                     VSGVIQIQSDMKNYFNDDLIAEIKVYIDNITPVISNIRLLESLNNMASIDTLTKAYNR
                     RYLEKYLEEQIKISKENNLHLSIIMIDIDFFKIFNDTYGHDAGDYVLMYFADTLKFNV
                     READVVSRYGGEEFIVVLPRTNHRGAYRVAEKLRKKVEEMSLTAISNENPPKITCSLG
                     ISVYPLHGNNIDMLIHSADKALYKAKNSGRNRTCISGDDNEREGIQERHDDINNTI"
     gene            3048..4289
                     /locus_tag="PKI50_11820"
     CDS             3048..4289
                     /locus_tag="PKI50_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003236581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT04427.1"
                     /translation="MNESFSMVAKHVNTVRSEDKIFALNREAGNAVLQFGKEKVVNAT
                     VGSILDEDEKLAVLPSVLETLRNLPDSEFAAYAPILGTPDYLESAIQAAFKEYRPEGY
                     IKAAATPGGSGALRQTIWNYSEIGDTILTSDWYWGPYKTLAEENLRKLSVFSLFDNEK
                     RFNLDSFEMKVKELLTRQNSLVILLNSPAQNPTGYALSDEEWEQVIMIIKREALDESK
                     KITLICDIAYIDYAGKASDTRRFMRLFSGLPSNVLVVVAFSISKSLTMYGLRTGAAVC
                     VSSDKGVAEEFSGVFEASNRGTWSNGTRCGMKLLSVIMNDKELLRKVDEEREQVRLLL
                     DKRAEVFTNEAKSVKLDICPYNAGFFVTIPAAQPEESCKLLKKDNIFAVPISRGIRFA
                     ICSVPLYKMKGIAEKIRKAVK"
     gene            4428..4940
                     /locus_tag="PKI50_11825"
     CDS             4428..4940
                     /locus_tag="PKI50_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04428.1"
                     /translation="MGKKFKCPMMCDMHMMHGVAPMDMMPYMPLAEEIEMDNFYEDEE
                     DDRHFMKMYPESCRKIMIYVKVEVDRMEGKDKEIHDYRPDREMINMMTDNAYNRMVKD
                     MPEMAEGEETRQYPARRFSRDLLRLLLLNELFRRRRRRRRRNSYGYPFGGYDFDFDND
                     DFDFDYDNYD"
     gene            complement(4931..5593)
                     /locus_tag="PKI50_11830"
     CDS             complement(4931..5593)
                     /locus_tag="PKI50_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNT04429.1"
                     /translation="MAASYRMLKGYADTCFVEKKSRFISYAQPVYSEEEALQFLAGIR
                     KKHYDATHNCYAYILGESMNIQRSSDDGEPSGTAGVPILEVLKKEELTNSIVIVTRYF
                     GGIMLGAGGLIRAYTQGAVNGIKAAGTVRMQPFAVYELKMDYSLLSKLQYEISKKDYI
                     INSIEYLENVTMRVLAASDLKDAFEKDIAQWTNGGILTESLGEQMLKVDEESGMIVTN
                     QS"
BASE COUNT         1852 a          979 c         1388 g         1539 t
ORIGIN      
        1 ctatggagga agcagtttgg aaaaggaaat aaaaaaactg atgcaaatat cagcaattat
       61 aacgagtatt ttattaatta cagcattaat atccttttta tatgtattaa agatatatcc
      121 cgcttcgaac cgaaggataa tcgtctatat gattgccggc gtatcggtgt ctttgctgtt
      181 aatatggggg gtatccctgg aattgtcaag agcatacaga acaggcaagg cgaagggttt
      241 cagcatatat gtactggaag cgggaatcgg tattctgctg ccgttatata tttatttgac
      301 aggattattc agcggagaca aggatgatat cagaaggtta tatattaaca ttaataatat
      361 tgcggtaagg cataatcttc aaaagaaccg cccatccaaa atacttatgc ttctccccgg
      421 ctgcatgcag gacaaggatt gcagctgcaa gataaccgat gacatgagaa attgccgcag
      481 gtgcggcgga tgcaggattg gagaagctgc agacctggtt gaaaagtgtg ctgtcagggc
      541 catagttgta aagggcggga cagcggccag aaataccgca aaggaattca agcctgattt
      601 catacttgct gttgcatgtg aaagagagtt attgagcggt atggcagatg taggcaggac
      661 accggttatg ggcgtaataa accagaggcc aaacggatac tgtaccaaca cgacagttga
      721 tatgctgcag cttaaggaaa ttcttgcgga gctggaggaa aaagggccgg agttaagatg
      781 cacaaaacaa ggagtaaaaa gagatgtaaa ggttcagaag aattattgat tgtcgattcg
      841 cttttggaga cgaaatagag gatattaata tcatgtcgaa tgctgtcgat atattaataa
      901 aggcaatgaa ttgatgtaaa gcatgcatta tgagggggat ggcaatatgg aagataataa
      961 gtcatatata acgaaatctc ttcttttatg gggcattttg ctggttatca gtatttttct
     1021 cggatattcc atatacaatg ctttttccgg ccgcatttat gcaaatgttt ttaaatccat
     1081 gaacaacaac ctgagatttg cagaaagctc ctacggcgta tttatgaatc agatgaaaat
     1141 gggcatgctg caggcatcgg tagaaaagaa tattaaagag atgattactg aaaaggatag
     1201 cgctgccctg gaggatttgc ttaaaaaatg gggagagcac agggactatg tagatgaatg
     1261 gtatgttgta ggagaggacg ggaatatcat atcttcggcg ggaaagggaa aagacctgct
     1321 gataaaggcc tccggcaaaa caaagattca agagattgta ggagaagcat tgtcatccgg
     1381 aactgttatt acaggtacgg aactgattcc tataggcagc aggggtgagt ttaccttggt
     1441 tcaatatgtc atagtacctg tgatggaaaa tgataggggt gtcatagggg gaatcgtaac
     1501 attgatgaac cttgacgggg atgataatat aaccggaaag ataatgagtg atacaggcat
     1561 gcacagtata atatccgcaa aggatcggat tattgcaagc agtcttgaat cagagctgta
     1621 cagattaaac aaaggaagca gtttgccgga agatatccgt aaggaagtgc ttgaaaagga
     1681 tacgccattt gttaagaggc ttgagatttt cagaggaagc cgggaggata aagactcata
     1741 ctttggtgca ttcagggcta taaaggatat tgaaggaagt ataataggag tccagggaat
     1801 tattcataat gaccctttgg ctgaaactac acttggacaa ataagggatt ttaccgttat
     1861 tgcagttata ctgctggctg cagttttttc aataatcata tggtcttata tcaatgcaca
     1921 gaaggttctt cttaaggaga agcagttttc aaacagactg agcagcataa aaaggttttc
     1981 ggatttggta aggcaagctt ccaatgaaga tgaggtatat gaaatattgt ttgacgtgct
     2041 gaagaaaaaa agcggcataa ctcaagttgt ggtggcgaaa aaggagtaca atgacaaatg
     2101 cttgacatta tataaggcca tggatatgga gaagatccaa tctataaaaa acacaatgac
     2161 ggttgaagag aactgctggg ctgtaaagtc aggaaaagaa ttcataaata acggcaataa
     2221 caatgatttt gactgcaagg atttctacag tgaatcagca agctatatct gccttccgat
     2281 aatactggga ggtatagtct caggggtaat tcagatacaa agcgatatga agaattattt
     2341 caatgatgat ctgatagcag agataaaagt atacatagat aacattactc ctgtaatcag
     2401 caacataagg ctgctggaaa gcctcaataa tatggcttcc attgataccc tgacaaaggc
     2461 atataacaga aggtatcttg aaaaatacct tgaggagcag attaaaatat caaaagagaa
     2521 taaccttcac ttgagtataa taatgattga cattgacttc ttcaagatat tcaatgatac
     2581 ttacggccac gatgcagggg attatgtact gatgtatttt gccgatacct tgaagttcaa
     2641 tgttcgggag gctgatgtag tgtcaaggta tggaggagag gaattcatag tggtacttcc
     2701 aagaacgaat caccgcggag catatagggt tgcggaaaaa ctaagaaaaa aggtggagga
     2761 aatgtccttg accgcaataa gcaatgaaaa tcccccgaag ataacctgca gtctggggat
     2821 atctgtatat cctctgcatg gcaataacat agacatgctt atacattctg cagacaaggc
     2881 gttatacaaa gcaaagaact caggcaggaa cagaacctgt atatccgggg atgacaatga
     2941 acgggagggg atacaggaaa ggcatgatga cattaacaat accatataat atatgttaat
     3001 aaaaacattt atatataatc aatacatata gaactggagg aaaaaatatg aatgaatcat
     3061 tttccatggt tgcgaagcat gttaatactg tcaggtcaga ggacaagata tttgctttaa
     3121 acagagaagc aggcaatgca gtactgcagt tcggcaagga aaaggtagtg aatgccaccg
     3181 taggctctat acttgatgag gatgagaagc tggcggtttt gccttcagtg ctggaaacac
     3241 tgagaaatct tcctgactct gaatttgcgg catatgcacc catactgggg actcctgact
     3301 atcttgagtc ggcaatccaa gctgccttca aggaatacag gcctgaagga tacataaagg
     3361 ccgcagcaac tcccggcggc agcggagctt taagacaaac aatctggaat tactcagaaa
     3421 ttggagatac gatactgacc tccgactggt actgggggcc atataagact cttgcggaag
     3481 agaacttgag gaagttaagc gttttttctc tttttgacaa tgaaaaaaga ttcaatcttg
     3541 attcatttga aatgaaagtc aaagaactgc tgacaagaca gaacagcttg gtaattctgc
     3601 tgaattcccc tgcacagaat cccaccggtt atgctctaag cgatgaagag tgggagcagg
     3661 ttattatgat aataaaaagg gaagctttgg atgaaagcaa gaaaattaca cttatttgtg
     3721 acatagcata tatcgactat gcaggaaagg cttcagacac aaggagattc atgaggcttt
     3781 tctccggatt gccgtcgaat gttctggttg tagtagcctt cagtatatcc aagagcctta
     3841 cgatgtatgg gcttcgtaca ggggctgccg tatgcgtaag ctccgataaa ggtgtagctg
     3901 aagagttttc cggtgtcttt gaggcttcca acagaggtac ctggtccaac gggacaagat
     3961 gcggaatgaa gctgctttct gtcattatga atgataaaga gctgctgcgc aaggtggatg
     4021 aagagaggga acaggttagg ctgctgctgg ataaaagggc ggaagttttt actaatgaag
     4081 caaaaagcgt caagctggat atatgcccgt ataacgcagg attttttgtc accatacctg
     4141 ccgcacaacc tgaagaaagc tgcaagctgc tgaaaaagga caatatattt gcagtaccga
     4201 tttcaagggg gataagattt gcaatttgct cggtaccttt atacaaaatg aagggtatag
     4261 ctgaaaaaat aagaaaggca gtgaaataga ttgactcaaa cccatctttc gaggaaggat
     4321 gggttttttc atgtaaaaat atggatgcac attgcaataa gacttgcact ttcccaaaac
     4381 cgtttcatat tactggtatt atgatgaata agtttggagg ttgtcaaatg ggaaaaaagt
     4441 tcaaatgtcc gatgatgtgc gatatgcata tgatgcatgg agttgctcct atggatatga
     4501 tgccttatat gccgttggca gaagaaattg aaatggataa cttttatgag gatgaggaag
     4561 acgacaggca ttttatgaag atgtatcccg aaagctgcag gaaaataatg atatatgtaa
     4621 aagttgaagt tgacaggatg gaagggaagg ataaagaaat acacgattac cgccccgata
     4681 gggaaatgat aaatatgatg acggataatg catataacag aatggtgaag gatatgcctg
     4741 aaatggcgga gggggaagaa accagacagt atccggcaag aaggttttca agggatcttc
     4801 tgcgtctgct gttactgaac gagcttttca gaagaaggcg aagacgcagg agaaggaatt
     4861 cctatggcta ccccttcggc ggatatgatt ttgactttga caatgatgat tttgattttg
     4921 attatgataa ttacgactga ttagtaacta tcattccgct ttcttcatcc actttgagca
     4981 tttgttcgcc cagggactct gtcagaatac ctccatttgt ccactgggct atatcttttt
     5041 caaaagcatc tttaaggtct gatgctgcaa ggactctcat tgttacattc tcaagatact
     5101 ctatgctgtt tattatataa tccttcttgg atatctcata ttgcagcttg ctcaaaaggc
     5161 tgtagtccat tttaagctca tatactgcaa agggctgcat cctcacagtg ccggcggctt
     5221 taatgccgtt gaccgctccc tgggtatatg ccctgataag ccccccggct ccaagcatta
     5281 ttcctccgaa gtatctggtg acaattacta tggaattagt gagttcttcc ttttttaaga
     5341 cctcaaggat aggaactccg gcagtccctg aaggttcccc atcgtcgctg gatcgctgta
     5401 tgttcattga ttccccaagg atataggcat agcagttatg tgtagcatca taatgtttct
     5461 ttctgatacc tgctagaaat tgaagggctt cttcttctga atataccggc tgggcatagg
     5521 atataaatct tgatttcttt tcaacgaaac aagtatcagc ataacctttt aacattctat
     5581 agcttgctgc cataatatca cctgcatctc tgtcttttgc tgtatattat tatacaacat
     5641 tatggcaaaa atcactattt gcgaaatcca aaggtttttg ccgcaacagg gcataatatg
     5701 aataatgcga cgcattcttc gggtacgagg tacgaggcgc gaggttcgag gtttaggg
//
LOCUS       DAOQUR010000094         5748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_15406_length_5748_cov_7.757597, whole genome shotgun sequence.
ACCESSION   DAOQUR010000094 DAOQUR010000000
VERSION     DAOQUR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15406_length_5748_cov_7.757597"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            910..1125
                     /locus_tag="PKI50_11835"
     CDS             910..1125
                     /locus_tag="PKI50_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNT04430.1"
                     /translation="MRTIKVGKKYRHFKGNEYLVLHLAKHSETLEDLVVYQALYGEQG
                     IWVRPLAMFLEQVKVEGKLVNRFEECD"
     gene            1144..1749
                     /locus_tag="PKI50_11840"
     CDS             1144..1749
                     /locus_tag="PKI50_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04431.1"
                     /translation="MASVKKPDTIDDITLHEAIKRVINECGPAKVSEITAKVNEKGLY
                     RRLDGNPVPANQVSARIRKYPHMFIRESGKVYLNEKNRILTVEASVGGYFGSSYQVYI
                     DLENKTASYKNLEGGYEIISDRELIIEDTAIEKFRFEMESIRILEWEKEYFVPILDGT
                     SWSVTIKTEGGLFESNGANAFPRNWSNFCNSIEKIVMGEFR"
     gene            1890..2855
                     /locus_tag="PKI50_11845"
     CDS             1890..2855
                     /locus_tag="PKI50_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNT04432.1"
                     /translation="MSNKVLDGIMGLCVADALGVPVEFTGREMLRKNPVTGMRSYGTY
                     NQPAGTWSDDTSMTLCLADSLSKGLDYNDIMKRFIMWFEEGKYTPHGEVFDVGNATRK
                     ALMSFLSGSAPLECGGLSDHDNGNGSLMRILPAVYYLRANYGTEITENEEAMEIIHNL
                     SALTHAHKRSLVACGIYCSVAAMLIGSMKLSTAVSLGINKAVEFYRRHKEYSDELSHY
                     ERLNNKSFAAVPVEEIKSSGYVVDTLEAAVWCLLNSNNYKECVLMAVNLGEDTDTVAA
                     LAGGLAGLYYGYDSIPQEWLSVVVRRDYVEGICSKLYEALSQRNF"
     gene            2881..3576
                     /locus_tag="PKI50_11850"
     CDS             2881..3576
                     /locus_tag="PKI50_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNT04433.1"
                     /translation="MRYTVDEAVFEINPEIKFGILIGSNIKNSETSAEDEKRLREAEE
                     NMRKTVKAEEVRALPNVALYREVMAKAGINPNKFLPSVEAMFKRILKGGALPKINALV
                     DLCNAVSIEQIISLGAHDLSDIHEDLEVCFSREGDKFIPFGSEEPEMVEEGELVFKSG
                     NYVQTRKWIWRQSELGKTTLDSKDIFFQLVGFGDDPSLNNAMAGIEDLIINRFGGSSK
                     KYLVDINNRYIDF"
     gene            3675..4766
                     /locus_tag="PKI50_11855"
     CDS             3675..4766
                     /locus_tag="PKI50_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04434.1"
                     /translation="MAQKGRVKKVFPGGNTSRGFFSFYDYIIPEDAERIFVIKGGPGT
                     GKSSFMRTIGEEFLKLGYDVELHYCSSDNKSLDGVVIKKANVALLDGTAPHIVDPKHP
                     GAVGKILNLGEYWDESRLVKDKYYIIDCNKQIKMRFESAYRYLRAAKEMQDDMEEIIG
                     EGVDREKLNKLKTELRYELVDGVSAINTNAAARHLFDSSITPEGLVDYVESIIQSNYI
                     CYYLKGGAGTVSTEILSFLADEYIMKGYGIELYHQPLNPDRLQTLVVEGLKVGITINP
                     KTEMKAYKIIDLDTIIDEGKFKDKAELLQKDAETYLRMLEEGVRRIKMAKLIHDDLEK
                     KYVGCMDFGAVTELRNNTVERIKGILKNK"
     gene            4815..5684
                     /gene="nudC"
                     /locus_tag="PKI50_11860"
     CDS             4815..5684
                     /gene="nudC"
                     /locus_tag="PKI50_11860"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNT04435.1"
                     /translation="MLYNILSDVIIQKVKVLIVDFILGIVPPEEEDKASFLFLFRQRN
                     LLVKKLEKGCMVPDSDDLALPGLDADKLYIGSMDGHSCYTASYPEAAPVPGGMEFVGL
                     RELYGQIDECMLKAAFRAVQILEWDETHRFCGACGEATERRTEEHARVCNKCGQIFYP
                     RLSPAVIVAVTKGDKLLLARNKSFPSGRYSVLAGFVEAGETLEECIKREIKEEVGIEV
                     KNIRYFGSQSWPFPHSYMLAFTAEHDKGEIRIDDSELADAGWFGADEMPQIPGSISVS
                     RKLINWFMEKYRK"
BASE COUNT         1850 a          892 c         1518 g         1488 t
ORIGIN      
        1 tctgatgaag ggttcaataa gaagattgaa gagttgctag gtgtgaaact tgatgatctc
       61 gtcaaatatg aaaatgcctt tggtgtagat aagggatatt agtgaggggg gggtccacat
      121 accttttaga ttggagttga tacagtggat aatatgactg attttatatc tatctgtgag
      181 ataaaacctt tgattgataa atatgggaga ataattgaat atcaacccga tagggaatat
      241 aaaaatgcag acgataaaaa acttaataag tatggcaaag gtccattttg taaatttcag
      301 atccccaatg gcataaataa agcaggagtc tatattctta aaatcgatga tgaagtcaag
      361 tatgttggcg agtgcgaaaa cttaagcagc agatataaca ttgggtatgg tcaaatttct
      421 cccagaaact gctatgtggg agggcagtct actaactgca agataaactc atacatatta
      481 caggaagtta aaaatggttc taaagtctat ctcttgttct atgaaactga gaataggttt
      541 gaagttgaga ggagcttaat aaaaaagtat gagcctgagt ggaatgccac ttcaggaaaa
      601 tatatctatg ctgttgaaaa gaagcctgta aataatggct ataaggaagt gaagaatgtg
      661 agtaatgcag gtaaatatta tctacttgaa aaatacttag acagacaatc tgcttctcaa
      721 attacattaa cattctcgga ggtagaaaga attatagggg agccgctgcc tgcttcagca
      781 tataaatatc ctgcttggtg ggcaaacggc ggtcattctc aggcagatgc ttggctgaat
      841 gccggttgga aagttggaga gttgaacctt gggcagtcag tagtttttgt aaaggataag
      901 caggcaacta tacgcactat aaaagtaggc aagaaatata ggcattttaa agggaatgag
      961 tatcttgttc tgcacttggc aaagcattct gaaacacttg aggatctcgt agtttatcag
     1021 gctctgtatg gagaacaagg aatatgggtt agaccccttg cgatgtttct agagcaggta
     1081 aaggtagagg ggaaattagt taataggttt gaagagtgtg attaataagg aggagggatt
     1141 ggattggcct ctgtaaaaaa gcctgacact attgatgata ttactttaca cgaagcgata
     1201 aagagagtta taaatgaatg tggtcctgct aaagtttcag aaataactgc gaaagtaaat
     1261 gaaaaaggat tatataggag attggatgga aaccctgtgc cggctaatca ggttagtgcc
     1321 cgaatacgca aatatcccca tatgtttatt agagagagcg gaaaagtgta tttaaatgag
     1381 aagaatagga ttcttactgt agaggcatct gtgggcggtt attttggcag ttcatatcag
     1441 gtatatattg atttggaaaa taaaacagca tcctataaaa accttgaagg cggatatgag
     1501 attataagtg acagggaact aataatagaa gatacagcaa ttgaaaaatt tcgctttgaa
     1561 atggaaagta ttcggatttt agaatgggaa aaggaatact ttgtgccaat ccttgatggg
     1621 acaagttggt cagtgactat caaaaccgaa ggagggttat ttgaaagtaa cggagcaaat
     1681 gcttttccga gaaattggag caatttctgt aatagcattg aaaagattgt tatgggtgaa
     1741 ttcaggtgat tggctgtatt ttgaggtgtg gattaaagag gaagttgaat atgttgctga
     1801 tatattggct gaaatatgaa ggcaaaccgg aatcaatgag aaagtaaagc acagctaggt
     1861 atttgccaat gccagaggag ggcctgacta tgtcaaacaa agtacttgac ggaattatgg
     1921 gcttatgtgt tgccgatgcc ttgggggtac cggttgaatt cacgggcagg gaaatgttga
     1981 gaaaaaatcc cgtgacaggt atgcgttctt atggtacata caatcagccg gcaggtacat
     2041 ggtcggatga taccagtatg actctctgcc tggcagacag cctttcgaaa gggcttgact
     2101 ataacgacat tatgaaaagg tttataatgt ggtttgagga agggaaatat acaccccatg
     2161 gagaagtgtt tgatgtagga aatgcaacac gtaaagcact catgagtttt ctatcgggat
     2221 cagcaccctt agaatgcgga gggcttagcg atcatgacaa cggcaatggg tcacttatga
     2281 gaatacttcc ggcggtatat tatctccggg ccaattacgg cactgaaata actgaaaatg
     2341 aagaagcaat ggaaataata cataatttat cggctctcac acatgcgcac aagcgaagcc
     2401 ttgttgcatg cggcatctat tgttctgttg cagctatgtt gataggaagt atgaaacttt
     2461 caacggcagt aagcctcgga atcaataaag cggttgaatt ctacagaagg cataaagagt
     2521 atagtgatga actgagtcat tatgaaaggc tcaataataa gtctttcgcc gctgttcccg
     2581 tagaggaaat aaaaagcagc ggttatgtag tggataccct tgaagctgcg gtctggtgtc
     2641 tgttaaactc aaataattac aaggaatgtg tcttgatggc agtcaacctt ggtgaagata
     2701 cggatactgt ggcagcactt gccggagggc tcgcaggatt gtactatggt tatgacagca
     2761 ttccgcagga atggctgtca gtggtagtga gacgggatta tgttgaaggt atatgcagca
     2821 agctgtatga ggctctaagc caaagaaatt tttaatatat aaaatagcgg aggaaacaaa
     2881 atgagataca cagtggatga agcagtattt gaaatcaatc cggaaataaa gttcggaatt
     2941 ctgattggaa gcaatataaa gaattcggag acttccgcgg aagatgaaaa aagattaagg
     3001 gaagcagaag agaatatgag aaaaaccgtt aaggcggaag aggtcagggc attgcctaat
     3061 gttgccctgt acagggaggt tatggccaaa gccggtataa accccaataa atttctgcct
     3121 tctgttgagg ctatgttcaa gagaattctc aaaggagggg cactgcccaa aattaatgcc
     3181 ctggttgact tgtgcaatgc tgtgtctatc gagcagataa tttcactggg ggctcatgac
     3241 ctgtcggata ttcatgagga tcttgaagtc tgcttcagca gggaggggga taaatttatc
     3301 cccttcggct ctgaagaacc tgagatggta gaagaaggag aactggtctt taaaagcgga
     3361 aactatgttc agacaaggaa atggatctgg aggcagagtg aacttggaaa aaccactctt
     3421 gacagcaagg atatcttttt ccagctggta ggctttgggg atgatccgtc tttgaacaat
     3481 gcaatggccg ggatagaaga tttgataatt aaccgatttg gaggaagttc caaaaaatat
     3541 ttggtagaca taaataatcg atatattgat ttttagaagc cgtactgctt ccatccaaaa
     3601 gcacatatat taattacata atgcaatgga tttgcatatt atgtattagt attatttctt
     3661 ttggaggtat aagcatggca caaaagggca gggtaaagaa ggtttttccg ggaggcaaca
     3721 caagcagggg gtttttctca ttttatgact atatcatacc cgaggatgca gagaggatat
     3781 ttgtaatcaa aggaggcccc gggacaggaa agtcaagctt catgaggacc ataggagagg
     3841 aatttttgaa actgggatat gatgtggaac ttcattattg ctcctcagat aacaaatccc
     3901 tggacggagt ggtgataaaa aaggcaaatg tcgcactgtt ggatggaacc gctcctcata
     3961 tagtagatcc caaacacccg ggtgcggttg gtaaaatact gaatcttggg gagtattggg
     4021 acgagtcgcg gcttgtgaag gataaatact atatcattga ctgcaataag cagataaaaa
     4081 tgcggtttga gagtgcatac agatatttga gggcagcaaa ggaaatgcag gacgatatgg
     4141 aggaaattat cggagaggga gtagacaggg agaagctgaa taagcttaaa acggaactga
     4201 ggtatgaatt ggtggatggg gtcagtgcca ttaatacaaa tgcagctgcc aggcatcttt
     4261 ttgacagttc aataactccg gaaggtttgg tggattatgt tgaaagcata atacagagca
     4321 actatatctg ctattacctg aaagggggag caggcacagt ctctaccgag atactctctt
     4381 ttcttgctga tgaatacatc atgaagggct atgggattga attatatcat cagcctctga
     4441 atccggatag gctgcagacc ttggttgttg agggtttgaa ggtgggaata accataaatc
     4501 ccaaaacaga gatgaaggcg tataaaataa tagatctgga tactattatt gatgaaggca
     4561 aattcaagga taaggcagag cttctccaaa aggatgcgga aacatacctc cggatgctgg
     4621 aggagggtgt caggaggata aaaatggcaa agcttatcca tgatgactta gagaaaaaat
     4681 atgtaggctg catggatttc ggagcagtta cggagctgcg gaataatacg gttgagagaa
     4741 taaaggggat attgaagaat aaataaatca aacaaatcag agcctcggga gttgatatac
     4801 ccgaaggttc ttttttgttg tataatatac tgagtgatgt aatcatacag aaagttaagg
     4861 tgttgattgt ggattttatt cttggaattg ttcctcccga agaggaagac aaagcatcat
     4921 ttttattttt gttccgacag agaaacctgc tggtaaaaaa gctggagaag ggatgtatgg
     4981 tgcctgactc tgacgacttg gcacttccgg ggcttgatgc ggacaagctg tatatcggca
     5041 gtatggatgg ccattcctgc tacactgcga gttatccgga agcagccccg gtgcccgggg
     5101 gaatggagtt tgtgggactg agagagcttt acgggcagat tgatgaatgt atgctgaagg
     5161 cggcattcag ggcagttcaa atattggagt gggatgaaac ccacaggttc tgcggcgcct
     5221 gcggagaagc cacagaaaga agaaccgaag agcatgcaag ggtatgcaat aaatgcggac
     5281 agattttcta tccgcggctg tctcccgcag ttattgttgc cgtaacaaag ggggataagc
     5341 tgctcttagc caggaataaa agctttcctt cggggcgtta tagtgtgctg gcaggctttg
     5401 tcgaagcggg agagactctt gaagagtgca taaagcggga aataaaagaa gaggtaggca
     5461 tagaggtaaa aaacatacgg tatttcggca gccagtcctg gccttttcct cattcgtaca
     5521 tgcttgcatt tacggcagag catgataaag gcgagatacg aatagacgat agtgagcttg
     5581 cagacgccgg atggttcggc gcggatgaaa tgccgcagat accgggatca ataagcgtat
     5641 caaggaaact tattaattgg ttcatggaaa agtatcggaa atagcaaaat aagacacggg
     5701 aagacacagg gacgttttgg gacatgggga cgcgggacac ggggacgt
//
LOCUS       DAOQUR010000095         5475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_16401_length_5475_cov_10.892066, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000095 DAOQUR010000000
VERSION     DAOQUR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16401_length_5475_cov_10.892066"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKI50_11865"
     CDS             <1..652
                     /locus_tag="PKI50_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNT04436.1"
                     /translation="AIIIGGIAGSFFRKGISERFSNIIMSVLGLFTLVLGMMFAVDTQ
                     NALVVIFSLVIGSVIGEWIDIEKRMNDLGDYVQGKLNSGEGSFSKGFVTASLLFCVGT
                     MSIMGSLQSGLMNNHNILLTKSILDGTISVVFASTLGIGVVLSSLPVLVYQGSIALLA
                     SSVAPYLSEAVITEMTAVGGVLLVGMGINLLETRKLKVGNMLPAIFLPIILMLFMK"
     gene            915..1199
                     /gene="groES"
                     /locus_tag="PKI50_11870"
     CDS             915..1199
                     /gene="groES"
                     /locus_tag="PKI50_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNT04437.1"
                     /translation="MNIKPLGDRIIIKVIENEETTKSGIVLPGTAKEKPMQGEVLAVG
                     SGEMVDGKKIPLELKAGDKVIYSKYSGTEVKMDGNEYLIIRQSDVLAIIE"
     gene            1217..2845
                     /gene="groL"
                     /locus_tag="PKI50_11875"
     CDS             1217..2845
                     /gene="groL"
                     /locus_tag="PKI50_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514589.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNT04438.1"
                     /translation="MAKQIRFAEDARRALESGVNQLANTVKITLGPKGRNVVLDKKFG
                     SPVITNDGVTIAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     LKNVAAGANPTIIKRGISKAVDTAVEELKKNSRPIESKEAIAQVASISAADETIGELI
                     AEAMEKVGKDGVITVEESNTFGTTLDVVEGMQFDRGCISLYMVTDTEKMEAVLDDPYI
                     LITDKKISNVQELLPILEQIVQQGKKLLIIAEDVEGEALSTLIVNKLRGTFTCVAVKA
                     PGFGDRRKAMLEDIAVLTNGQVISEELGLELKETKLSQLGRARQVKVQKENTIIVDGA
                     GEPKAIKDRIKQLKVQIEETTSDFDREKLQERLAKLSGGVAVIKVGAATETELKERKH
                     RIEDALAATRAAVEEGIVAGGGTALANTIPAVNALIAGADGDEKTGIRIVKRALEEPV
                     RQIAENAGLEGSVVIEKVMASKPGIGFDALKEEYTDMIQAGIVDPTKVTRSALQNAAS
                     VAALLLTTEGVVADLPEKEKPMMPGGGMGGDMMY"
     assembly_gap    2912..3011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3019..5385)
                     /locus_tag="PKI50_11880"
     CDS             complement(3019..5385)
                     /locus_tag="PKI50_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNT04439.1"
                     /translation="MFKISSNSIMFKVLVMILTICMLESLLFIIMSERLTGSILENMT
                     IENSSKNAELYSEFIGNWLGERMREINMYANSPIIRTMDWNSIEPYLRQEVNEKLNVY
                     DHFMVAESNGDYNTTLRRNVGNVSDREYFKHVMEGKAVVSNPIVSRSTGKPEAIIASP
                     ILDNNGKIKGVMAGAVNLMKLSRIIDNLKYNYPNSYSYIVDKEGQIIAHPREELILRH
                     NITKESDVISRDIAEASAEILTNDRGSNTYSFEGIVSMNYYHIIPNTDGWKLIIKIPV
                     NYWQTPMRYTSSRLILIGLAGLIIASIVGFFIAKSISTPIIKLKEVFTRATAGDLTVR
                     SEISTDDEIGDAARSFNKMMDTISKLTYYDSLTLLPNKLHFDAILCREIKKSASNNTM
                     LALIIFDIDRFESINNALGHTVGDKLLKSLAEKVSCLEDNNHVVSRIGEDMFAVLLKN
                     FKEKEDVIKLSDEIRYIIRQPWVIDEYRFYITACVGIAIYPKDGESGDSLFKNAFSAM
                     QKAKVHGRDNFNFYEPSINELLVEQMNLDSSMHHALDNGEFSIYYQPQMDTRTQEIIG
                     CEALLRWNHPELGMIGPNEFIPISEANGLITLIGRWVLYTACMQNKLWQDSGFKPIFV
                     SVNLSPAQLIQENFIDMVSEILEETKLLPEYLELEITESVAVKNYDYITGILEKLKKM
                     GIRIALDDFGTGYSSLNYLKNFAITTLKIDRSFIHDINENPKNSAIVSSILAMGRNLK
                     LCITAEGIETKEQYETLRDRGCDIMQGFYFSKPLPLHEFEKLMKKPGD"
BASE COUNT         1581 a         1015 c         1242 g         1537 t
ORIGIN      
        1 agcaataatt ataggcggta ttgcgggaag ctttttcaga aaaggtatat ctgagcgttt
       61 cagcaatata atcatgtcgg ttctggggct ttttactctg gtgctcggca tgatgtttgc
      121 ggttgatacg caaaatgcat tggttgtaat attcagtctc gttatcgggt cggtaatcgg
      181 agaatggatc gatatagaaa agagaatgaa cgatttaggc gattacgtcc agggcaagct
      241 taattccgga gaaggaagct tttcaaaagg ctttgtaact gccagccttc tattctgcgt
      301 aggaacaatg tcaattatgg gatcgctgca gagcggatta atgaataatc acaatatact
      361 gctgacgaag tctatattgg acggaacgat atctgttgtt tttgcatcaa ctcttggaat
      421 aggggttgta ctgtcgtccc tgcccgtttt ggtgtatcag ggctccatag ctttgcttgc
      481 gtcatccgtt gccccatacc tcagtgaagc ggtgattact gaaatgacag ccgtaggagg
      541 agtcctgctt gtgggaatgg gcataaattt gctggaaaca aggaagctga aagtcggcaa
      601 tatgctgccg gcgatattcc ttccgataat actgatgtta tttatgaagt aaagtcgcgg
      661 ggctggagat acgggatacg atgaaccctt atgccttgaa atattaagaa aatggtataa
      721 atacggcgta atgttgaaat aatataaggc attggaggaa tattatcatt atttgctgtt
      781 tttggcttat ttgggagttt gataatataa tttcctttaa ttattatata aagtgtaatt
      841 agcactcagt ctaggtgagt gctaataata aaaatacata gtgtttaaag taattacttt
      901 aaggaggagt atgcatgaat atcaaaccct taggcgacag aatcatcatc aaagtcattg
      961 aaaatgaaga aacaaccaag agcggtatag tgcttccggg tactgcaaag gaaaaaccga
     1021 tgcagggcga agtgcttgcg gtaggttccg gagaaatggt ggacggcaag aaaattcctc
     1081 tggagttaaa agcaggagat aaggttatat actcaaaata ttccggaact gaagtgaaga
     1141 tggatggcaa tgagtatctg atcatcagac agagcgatgt tttggcaata attgaataaa
     1201 ttaggaggga atatagatgg caaaacagat aagatttgca gaggatgcaa gacgggcact
     1261 tgaaagcggt gttaaccagc ttgcgaatac agtaaaaata acattaggcc caaagggcag
     1321 aaatgtagtt ctggataaga aattcggctc accggttatt actaatgacg gtgttaccat
     1381 agcgaaggaa atagaactgg aagatccctt tgaaaatatg ggagcacagc ttgttaagga
     1441 agttgctacc aagacaaatg atgtggcagg ggacggtact actacagcta cacttcttgc
     1501 gcaggcaatc attagagaag ggctcaagaa cgttgcagca ggagcaaatc cgacgataat
     1561 aaagagaggt atcagcaaag cggttgatac ggcagtagaa gagcttaaga agaattcgag
     1621 gcctatcgag agcaaagaag ctattgcaca ggttgcttcc atttccgctg cagatgagac
     1681 aataggggaa cttattgctg aagctatgga aaaggtaggc aaggatggcg ttattactgt
     1741 tgaagaatcc aataccttcg gaacaacact cgatgtggtg gaaggcatgc agtttgacag
     1801 aggctgcatc tcattatata tggttaccga tacggagaaa atggaagctg tactggacga
     1861 tccatatata ctcattaccg acaagaagat ttcaaacgta caggaactgc tgccaattct
     1921 tgaacagata gttcagcaag gtaagaagct tcttattatt gctgaggatg ttgagggaga
     1981 ggccttgtca acattgattg tcaataagct cagaggcact ttcacctgtg tggcagtaaa
     2041 ggctccgggc tttggagaca gaagaaaggc aatgctggaa gatattgcag tactcacaaa
     2101 tggccaggtt atatccgaag aactgggctt ggagcttaaa gaaacaaaac tgagccagct
     2161 tggaagagca agacaggtca aggttcagaa agaaaataca attattgttg acggtgccgg
     2221 agaaccaaag gcaatcaaag acagaatcaa acagcttaaa gtacagatag aagagaccac
     2281 ttccgacttc gacagggaaa aactccagga aaggcttgca aagctttccg gcggcgttgc
     2341 ggtaatcaaa gtcggtgctg ctacagagac agagcttaag gaaagaaagc acagaataga
     2401 ggacgctctt gcagcaacaa gggcggcagt tgaagaaggt atagtagcag ggggcggaac
     2461 tgcacttgca aatacaatac ctgctgtaaa cgcattgatt gccggagcgg acggggacga
     2521 aaaaacaggc atcaggatag tcaagagggc attggaagag cctgtcagac agattgccga
     2581 gaatgcggga cttgaaggct cagtggtaat tgaaaaggtt atggcaagca agcccggaat
     2641 cggtttcgat gctttgaaag aagaatatac agacatgatt caggccggaa tagttgaccc
     2701 gacaaaggtt acaagatcag cacttcagaa tgcggcttcc gttgcagcat tgctgcttac
     2761 tacggaaggt gtggtggcag acctgcctga gaaggaaaag ccaatgatgc caggcggcgg
     2821 tatgggcgga gacatgatgt actaagatga aatagaggac agggggacgt ttcgcttgtc
     2881 ccgctttcag cgggacagga gaaacgtccc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn ntgtcctctt aatcaccggg ttttttcatt aatttttcaa attcatgcag
     3061 gggaaggggt ttactgaaat agaacccttg catgatgtca cagcccctgt ctctcaaagt
     3121 ctcatattgc tctttagtct caattccttc tgcggttatg cagagcttca gattcctgcc
     3181 cattgcaagt atgctggata caattgctga attctttgga ttttcattta tgtcatggat
     3241 aaagctgcgg tctatcttca aagtggtgat ggcaaaattc ttgagataat taagagaaga
     3301 atatcccgtt ccaaaatcat caagagctat tcttatgccc attttcttaa gcttttccaa
     3361 tatacccgtt atgtaatcgt agttttttac agcaacgctt tctgtaatct caagctccag
     3421 atattccggc aaaagttttg tttcttcaag aatttcggat accatatcga tgaagttctc
     3481 ttgtataagc tgtgcaggag ataaattaac cgatacaaaa attggtttga agcctgaatc
     3541 ctgccaaagc ttgttctgca tgcaagccgt gtagagaacc catctgccta taagggttat
     3601 caatccgtta gcctcagaga tggggatgaa ttcattgggg ccaatcattc ccagctcagg
     3661 gtgattccat ctcaacagag cttcacagcc aattatctct tgggtgcgcg tatccatctg
     3721 aggttgatag tatatggaaa actcgccgtt gtccaatgca tgatgcatac tgctgtccag
     3781 gttcatttgt tcaacaagca gttcgtttat tgaaggttcg tagaaattaa aattatccct
     3841 gccgtgtacc tttgcttttt gcattgccga aaatgcattc ttaaacaggc tgtcgccgct
     3901 ttctccgtct ttcggatata tggcaattcc tacgcaggca gtaatataaa atctgtattc
     3961 atctatgacc cagggctgcc tgataatata ccggatttca tccgacagct ttataacatc
     4021 ttccttttct ttaaaattct taagcaatac tgcaaacata tcctctccga tacgggaaac
     4081 tacatggtta ttatcttcaa ggcagctgac tttttctgcc aggcttttca gaagcttgtc
     4141 acccactgta tggcccaaag cattgttaat gctttcaaat ctgtctatgt caaatattat
     4201 taatgcaagc atggtattat tactggcgga tttcttaatt tccctgcaca gtattgcatc
     4261 aaaatgcagt ttattgggga gcagggttag tgaatcataa taggtcagct tgcttatggt
     4321 atccatcatt ttattaaagc ttcttgcggc atctccgatt tcatcgtcgg tactgatttc
     4381 cgaccttact gtaagatctc cggcggttgc ccttgtaaac acttctttaa gctttatgat
     4441 tggagttgat atgctttttg caatgaaaaa tccgactatt gaagctatta taaggcctgc
     4501 aagcccaatt agtataagac gcgatgaagt atatctcatc ggggtttgcc agtagttcac
     4561 aggtattttg ataatcagct tccaaccgtc ggtattgggg attatatgat aataattcat
     4621 gctgacaata ccttcaaagg aatatgtatt agagccccgg tcatttgtga gtatttctgc
     4681 cgatgcttca gctatgtctc tactgatgac atctgattcc tttgttatat tatgccggag
     4741 aattaattct tccctgggat gggcaatgat ttggccttct ttgtctacaa tatatgagta
     4801 tgagttggga tagttgtatt taagattatc aataatcctt gaaagcttca tgagatttac
     4861 tgctcctgcc ataactcctt tgatttttcc gttattgtcc agtataggtg atgcaataat
     4921 cgcttccggc tttccggtag atctggagac aatcgggttg gacactactg ctttgccttc
     4981 catgacatgc ttgaaatact cacggtcact aacgtttccg acatttcttc tgagagtagt
     5041 attgtaatcc ccgtttgatt ccgcaaccat aaaatggtca tatacattaa gcttttcatt
     5101 gacctcctgc ctcaggtaag gttctatgct attccaatcc attgttctta taatgggaga
     5161 atttgcatac atattaattt cacgcatacg ttcccccagc cagttgccta tgaattcgga
     5221 gtacagttct gcgtttttgc tgctgttttc gatggtcatg ttttccaaaa tgctgcctgt
     5281 gagtctttcc gacattatta tgaaaagtaa agattccaac atacatatgg ttaatatcat
     5341 caccagcacc ttgaacatta tactattact gcttatcttg aacattccgc tgtcctcctt
     5401 gatacagttg aaaaaatgat gataaaatac catccatgac aaattatatc aaaaattggc
     5461 atatttatat agtgt
//
LOCUS       DAOQUR010000096         5299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_17135_length_5299_cov_11.459001, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000096 DAOQUR010000000
VERSION     DAOQUR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17135_length_5299_cov_11.459001"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..711
                     /locus_tag="PKI50_11885"
     CDS             187..711
                     /locus_tag="PKI50_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT04440.1"
                     /translation="MDLVRIGEKIISIPRINNKINEILQYRMLGMSQQEAADKLGVER
                     TFISRLEGMGEIRRGQSIAAIGFPIKNRTEVEKVLRAFGVEYFFLMSEAERTDYVNNC
                     TGAQLTNRIIELVNKLRNFDIVIVMVSDYRNRLARNLLDNKVIEIDIGKSPIKEDVNV
                     NLDNLTDILKTLKG"
     gene            719..1660
                     /locus_tag="PKI50_11890"
     CDS             719..1660
                     /locus_tag="PKI50_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HNT04441.1"
                     /translation="MKHIVSISIGSSRRNHKVSMNINNEEIIVERIGTDGDIQMAIKL
                     IRELDGKVDAFGMGGIDVYLSAGKARYRIKDAVPILEAAKITPMVDGSGLKMSLEKDV
                     PGFINDNVEDITGKSVFIMPAIDRYGMAEGFQNLGCELVLGDLMVALGINIPIRSLRM
                     LDKIASIIAPIACRLPFEMLYPTGKEQNKGTSRAGKIDRFFYEADIIAGDFHYIRQYM
                     PGGMQGKMVVTNTVTDEDIEWLKKCGIRTLVTSTPNLGGRSFGTNVIEAVLVALIGKS
                     IDEITGEDYLGMLKEIDFKPRVEQFEEGELFKEEYIR"
     gene            1712..2554
                     /locus_tag="PKI50_11895"
     CDS             1712..2554
                     /locus_tag="PKI50_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04442.1"
                     /translation="MNNAAIIVFRKSTLPKILKFFAIPLVNRIIDSNNEKDSVRVYII
                     ECPVMKYPLDNRRVEKSLCSLCIENNISFFIGRNTVNYSGSGLEHMENRIERGDLDEI
                     KGMKGLAALIKLSVIRNSNLLNRNMCFIGKSYNYGYISTMLEEATGVFIYEHDQVTNS
                     IKKTIFEKLMLERGISAVFTKDLGRAISQCDIIAADDSVDLESYKPMLKGKILIGENT
                     VSGDFEKIERVLLWYESLEGVTEDNSIIYYNDELLGILRHFYKERNIIDFIRRFPYIL
                     MSRS"
     gene            2877..3350
                     /gene="greA"
                     /locus_tag="PKI50_11900"
     CDS             2877..3350
                     /gene="greA"
                     /locus_tag="PKI50_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNT04443.1"
                     /translation="MGKQNILTIEGLAKLEDELDFLKSKKRAEIAQRIKQALAFGDIT
                     ENSEYDEAKNEQAFVEGRIVQIENILKTAKVIDDEDIQTDIVSVGCRVTLRDVELGDE
                     VEYLIGGSAEADPINLKISNESPVGKALIGKTIGNIVDIVVPDGVIKYEILNIKK"
     gene            3362..4858
                     /gene="lysS"
                     /locus_tag="PKI50_11905"
     CDS             3362..4858
                     /gene="lysS"
                     /locus_tag="PKI50_11905"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNT04444.1"
                     /translation="MANDEMNLNEILKIRREKFEQLKAMGRNPFEILKYDQTHHSKDI
                     KGNFEEMEGKDVSIAGRIMSFRDMGKASFCDIQDRYGRIQAYIKQDELGEEEYKIFKT
                     YDIGDIVGIKGFVFRTKRGEISVHAKQVTLLCKSLQPLPEKWHGLKDTEIRYRQRYVD
                     LIVNEDVRETFYKRTKIIKAIREFLDARDFIEVDTPILATIASGAAARPFITHSNALD
                     IDLYLRIATELYLKRLIVGGFERVYEMGKDFRNEGIDVRHNPEFTMIELYQAYADYND
                     MMEITENLIAYCAEKVLGTTKINYQGTELDLTPPWNRMTMVEAVKKYSGVDFNEIKSD
                     EEARETAKKLKVLDELKKKLKDCTKGDILNAVFEAYVEEHLIQPTFILDYPVEISPLT
                     KKKNDDPTMTERFEAFIFAREIANAYSELNDPIDQKERFEQQANDKELGDEEAYATDD
                     DFVNSLEIGMPPTGGLGIGVDRIIMFLTDSYSIRDIILFPTMKPIAKD"
     gene            5052..>5299
                     /locus_tag="PKI50_11910"
     CDS             5052..>5299
                     /locus_tag="PKI50_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT04445.1"
                     /translation="MGEVEEKLAKSLTGETTELIPYLPYLLQDLWELGSDPKEIIKLI
                     RKNIRKVKDLKILDLACGKGAVSVKLAKELGCHVQGID"
BASE COUNT         1810 a          830 c         1303 g         1356 t
ORIGIN      
        1 gaaacgtccc cgtgtccccg tccccatgtc tcgcgtcgca ttcttcatat attgtgcaca
       61 aattccgccg tttattggaa aagctgtgga taaatttcca tattgagaat aaaatatata
      121 agtactaaaa tgaaattgta cacaacattg tgcacataaa taaaaaagtg tatttgaggt
      181 gttgctatgg atcttgtaag gataggggaa aaaataataa gtattcccag gataaacaac
      241 aaaataaatg agattcttca gtaccgcatg cttggaatgt cacagcagga ggcagcggat
      301 aagctgggtg tagagcgcac ttttatatca cggcttgaag gcatggggga gataaggagg
      361 ggtcaaagca tagcggctat aggttttccc ataaagaaca ggacagaggt ggaaaaggtg
      421 ctgagggctt ttggagtcga atacttcttt ctcatgtctg aggctgaaag gacagattat
      481 gtaaacaact gtaccggagc acaactgacc aacaggataa ttgagcttgt caacaagctc
      541 agaaacttcg atatcgtaat tgtcatggtt tccgattata ggaacagatt ggccagaaac
      601 ttgctggata acaaggttat agagatagat ataggaaaat cgcctataaa ggaagatgtc
      661 aatgtgaacc tggataattt aacagatata ttaaaaacat tgaaaggata gatatggtat
      721 gaagcatata gtgagtataa gtataggctc atccagaaga aaccacaaag tgtccatgaa
      781 tatcaacaat gaagagatta ttgttgaaag gataggcact gacggggaca ttcagatggc
      841 aatcaagctt atacgcgaat tggacggaaa ggtcgatgcc tttggaatgg gaggaatcga
      901 tgtttacctt agcgccggga aggcccgtta taggataaag gacgcagttc caatactgga
      961 agccgcgaag attacaccaa tggttgacgg ctccggcctt aaaatgtctc ttgagaagga
     1021 tgtaccgggc tttattaacg acaatgtgga agatatcacg ggtaagagtg tatttatcat
     1081 gcctgccatt gacaggtacg gcatggccga gggctttcaa aacctgggct gcgagcttgt
     1141 gcttggagat cttatggttg ccttgggtat aaatataccg ataagatctc tgaggatgct
     1201 ggacaaaata gcaagtatca ttgcaccgat tgcctgtcgg cttccctttg aaatgctgta
     1261 ccctaccggg aaggagcaga ataaaggaac cagcagggcg ggcaaaatag acagattctt
     1321 ttatgaagct gatattattg caggggattt tcattatata aggcaatata tgcccggcgg
     1381 gatgcagggg aaaatggttg ttacaaatac cgtcaccgat gaagatatag agtggcttaa
     1441 aaaatgcggg atacgtacct tggttacatc aacgccgaat ctgggaggcc gctcctttgg
     1501 caccaatgtc attgaggcgg tgctggtggc tttgataggc aaaagcattg atgaaataac
     1561 cggtgaggat tacttgggca tgctgaagga gatagacttc aaaccaagag tggaacaatt
     1621 tgaagaagga gaattgttta aggaagaata tatccgatag gcagttaata ttattatatt
     1681 agcaattttc aatatattcg gaggggatgc aatgaataat gcggcaataa ttgtttttag
     1741 gaaaagcact cttccgaaaa tattgaagtt ttttgcgata cccctggtaa accggattat
     1801 tgattcaaat aatgaaaagg acagcgtcag ggtgtacatt attgaatgcc ctgtaatgaa
     1861 gtacccttta gataacagga gggtggaaaa atccctttgc agcttgtgca ttgagaataa
     1921 catcagtttc ttcataggaa ggaatacagt gaattattcg ggaagcggcc ttgaacatat
     1981 ggagaacaga atagaaaggg gagatcttga cgaaattaag gggatgaaag gcctggctgc
     2041 ccttattaag ctttctgtta ttagaaactc taaccttctg aatagaaaca tgtgctttat
     2101 cgggaagtca tacaactatg gatatataag tacaatgctg gaggaagcga caggggtttt
     2161 tatttatgag catgaccaag tgaccaatag cattaagaaa acaatttttg aaaagctgat
     2221 gctggagagg ggaatatctg cggtgtttac caaagatctg ggcagagcga tttctcagtg
     2281 tgatattatt gcggcagatg acagtgtaga tctggaatca tataaaccga tgctaaaagg
     2341 taaaatatta attggcgaaa atactgtatc aggggatttt gaaaaaatag aaagagtgct
     2401 gttatggtat gaaagcctgg aaggagtgac tgaagacaac agcattattt attataacga
     2461 tgaactgctt ggaatattga ggcattttta caaagagagg aatattatag attttataag
     2521 gaggtttcct tacattttaa tgtctcgcag ctagtgctta ttaacataac aaattactaa
     2581 tctttcaaag tattaacagg catatcagcc ggagcatgca cctccttgct tattttagcg
     2641 ctttcactaa tgcttgacat aaggaaagca ctacattata ataaacaaat aaagaaaaag
     2701 aatgctgtgt cttcattgaa aatttataat tatggaaata cctgcacagc ataaaaaaat
     2761 tatttaagta catattatca taaataaaaa tattataggg tttcattgta ctggctgggg
     2821 aacaagccag attttatctg tataagtaat ttttatggaa cgggaggcag gagacattgg
     2881 gtaaacaaaa tatactaaca atagaaggtc tggctaagct tgaggatgaa ttggattttt
     2941 taaagagcaa aaaaagggcc gagattgcgc agagaataaa gcaggcactg gcttttggag
     3001 acataaccga aaattcggaa tatgatgaag ctaaaaatga acaagcattt gtagaaggaa
     3061 gaatagtaca gatagaaaac atccttaaga ctgccaaagt aatagatgat gaggacatcc
     3121 agacggatat tgtaagtgta ggctgcaggg taaccctgag agatgttgag ctgggcgatg
     3181 aggttgaata cttgattggg ggctcagctg aggcggatcc cattaatctc aagatctcaa
     3241 acgaatctcc cgtgggcaag gccttgatag gcaagactat cggcaatatt gttgatatcg
     3301 ttgtacctga cggagttatc aaatatgaaa tactaaacat aaaaaaatag ggggagtaag
     3361 catggctaat gacgaaatga atctcaatga gatactgaaa attagaaggg aaaagtttga
     3421 acagctgaag gcaatgggac gcaatccctt tgagatattg aagtatgatc agacccatca
     3481 ctctaaagat atcaaaggca atttcgaaga aatggaagga aaggatgttt ccatagccgg
     3541 aaggataatg tccttcaggg atatgggaaa ggcatcattt tgtgatatac aggacagata
     3601 cggaagaatt caggcataca taaagcagga tgaactgggt gaagaggaat ataagatttt
     3661 taagacctat gacataggtg atattgtcgg tataaagggt tttgtgttca ggaccaaaag
     3721 aggcgagata tcagtacatg caaagcaggt aaccttgctg tgcaagtcgc tgcagccgct
     3781 gccggagaaa tggcacgggc ttaaggatac tgaaatcaga tataggcaga gatatgtgga
     3841 tctgattgta aatgaggatg taagagaaac attctacaag aggaccaaga taataaaggc
     3901 aatacgggaa ttcctggatg caagggactt tattgaagtt gatactccaa tactggctac
     3961 aattgcgagc ggagctgccg caaggccttt tatcacccac agcaatgcgt tggatataga
     4021 tttatatctc agaattgcca ccgagcttta tttaaagagg ctgatagtcg gcggctttga
     4081 gagagtatat gaaatgggca aagacttcag aaatgagggt atagacgtaa ggcacaatcc
     4141 ggagtttacg atgattgagc tataccaggc ctatgcggac tacaacgata tgatggagat
     4201 taccgagaat cttatagcat attgtgcgga aaaggttctg ggaacaacaa aaatcaacta
     4261 tcagggaacc gaattggatt tgacaccccc ctggaacaga atgaccatgg tggaagctgt
     4321 caagaagtat tcgggtgttg actttaatga aataaaatct gatgaagaag caagagaaac
     4381 agcaaagaag cttaaggtgc tggatgaact taaaaagaag cttaaggact gcaccaaggg
     4441 cgatatattg aatgcggttt ttgaagcata cgttgaagag catcttattc agccgacttt
     4501 tatattggat tatccggttg aaatatcacc tcttacaaag aagaagaatg acgatcctac
     4561 aatgacagag aggtttgaag cgttcatatt tgccagagaa attgccaatg cttattccga
     4621 gcttaacgac cccattgacc agaaggaaag gttcgagcag caggcaaatg acaaagagct
     4681 tggagacgaa gaggcatatg caacggatga tgactttgtt aactcccttg aaatagggat
     4741 gcctcctaca ggaggacttg ggataggggt ggacagaata ataatgttcc ttacagattc
     4801 ctattctatc agggatataa tactgttccc gacaatgaag cctattgcaa aggattgata
     4861 aatgatatag atgggtttat cggaatccgg ccaaggatta aatcttgggc cggattttct
     4921 tattgtcttg aaacagtgaa agaaagggaa tattttataa taataataag tgtgatataa
     4981 tagaaaaaca tccagaataa gacagagaaa aatttccggc gctgcctgat aaactataag
     5041 ggagaattat tatgggagag gtagaagaaa aacttgcaaa atcattaacc ggtgaaacaa
     5101 cagaactgat tccctatttg ccgtatttgc ttcaggacct gtgggagctg ggctcagacc
     5161 cgaaagaaat tataaagctt ataagaaaga atatacggaa ggtgaaggat ctcaaaatac
     5221 ttgatcttgc ttgcggcaaa ggagcggtaa gcgtgaaact ggcaaaagaa ttgggatgtc
     5281 atgtgcaagg cattgatat
//
LOCUS       DAOQUR010000097         5111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_18000_length_5111_cov_13.702729, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000097 DAOQUR010000000
VERSION     DAOQUR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18000_length_5111_cov_13.702729"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1641)
                     /locus_tag="PKI50_11915"
     CDS             complement(343..1641)
                     /locus_tag="PKI50_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase"
                     /protein_id="HNT04446.1"
                     /translation="MYKNPYMCPMNPFYICPFQNLTEDTEYDDSFIEEYYDELYFEDE
                     YYEYGYEGMRQRPQDVSRIMPLINRRFAGHYQELERYGVRRSDTRNIFRNTVIYVLRN
                     ANNYNGTIEQRTNALLNALRRDNPYAFTILRRKGVPNARINQIMRDIIQFTLENIEVA
                     EGTGWSQWEDLGGILTSAPAAASWQPNRLDVFARGQNQALWHVYWNGARWSAWEDLGG
                     ILTTGPGEVSWGTNRIDVFARGQNQALWHIYWDGIRWSAWEDLGGIITSEPAAASWQA
                     NRLDVFARGQNQSLWHKYWDGIRWSEWEDLGGVLTSAPAAVSWGPNRIDVFGRGQNQS
                     LWHKYWDGTRWSAWEDLGGGQITSGPAAASTGSNRLEVFARGQRNELMIRTWNGTRWS
                     GWSPLGGVLTSDPAAVSWGGNRLDVFARGQNNHLWHIRRA"
     gene            complement(1802..2548)
                     /locus_tag="PKI50_11920"
     CDS             complement(1802..2548)
                     /locus_tag="PKI50_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04447.1"
                     /translation="MTILKKGQLTRHINESKEQDEYVMRIITPEELGDAVALQKYVYE
                     QLPNKQVLYMDSYEEMYDDMKQGAKIIGVFNKTGDIIAYRYIGFPGCSEKNLGNDINM
                     PRQELAKVAHLETTVVHPEYRGNSLQSLTLQQAIPMIKDFGYSHLLCTVSPQNIYSLY
                     NIIKNGLRIKALKKKYGSEKDGKDGMWRFILHRNLEPRAVRKVSQNFGIPIGDFELQR
                     KLIDEGFVGLWLYKESKLLNYVRFEDAIAN"
     gene            complement(2760..3398)
                     /locus_tag="PKI50_11925"
     CDS             complement(2760..3398)
                     /locus_tag="PKI50_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNT04448.1"
                     /translation="MNLKSHGEDKIPTAKYEKIALDIAYSILNEEWKVGEMVKGRSTL
                     SGKYSVSPETIRRALKLLEELQVVNVIEKKGILIKSKDAANNFIKEHQSKDRILSMRE
                     EIVKLMEEKQALEKQVLERLDSVIEQAMLLRNIGIIFPLELKIPEKSHLVGKTIGEVR
                     FWNHTGATIIGINRSGHLYLSPGPKMVFFPNDIILYVGSDAEISEKVAKYVK"
     gene            complement(3500..4870)
                     /locus_tag="PKI50_11930"
     CDS             complement(3500..4870)
                     /locus_tag="PKI50_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT04449.1"
                     /translation="MDTSKQLGEEKISKLLLKFSIPAIVGMLVNALYNVVDRIFIGNS
                     VGPLGIAGITIGFPIMLVIMAFAMLIGIGANSLISIRLGEQRKEEAELILGNAVVMLV
                     ITSMTISGTGLIFIEPLLRIFGASAAVLPYAKEYLSIILLGTVIQTVGFGMNNFIRAE
                     GNPRIAMFTMIIGAILNTILDPIFIFVFGLGIRGAAIATVLSQAVSTSWVLYHFFGGR
                     STLKVHTKNLRLHTPIVVKIVSLGAATFFMQMAASVLNAIMNKSLVIYGGDIAVSGMG
                     VIMSIAMLILMPIFGINQGAQPIIGYNYGALEFDRVKKALKLAVVAATIITTIGFILT
                     RVFPSQLILLFNRTDKDLLNFGTRAISIYLIFLPIIGFQIVSANYFQAVGKPKHAAFL
                     SLSRQVLILIPALLILPLFFGLEGLLMAGPASDLTSSVITGIFLIRELKNLDARHRDA
                     VVQEQN"
BASE COUNT         1392 a         1239 c          907 g         1573 t
ORIGIN      
        1 tttattattt tgatatgcta taaagtaggc tgttgtacaa aatatgaaga atgcgacgca
       61 ttcttcgggt acgaggtacg aggcgcgagg ttcgaggttt agggcagggc ttcggagtta
      121 tgataacact gaagacacga aaaaccgcta aatgcacggc gggataaata ttacgttaca
      181 taaataatta tcgataatga tgtaagtatc gaaattttct tatgtaacta tataatatat
      241 aaaagttacc gtgcaatcgc gcagcgattc catgaaacag ccgtgtctcc cgtgttaccc
      301 gttacctacg tcgcattatt cttctattgc gaaatcccca ttctatgctc tccggatatg
      361 ccacaaatgg ttgttctgcc ctctggcgaa tacatccagg cggttgcctc cccaggatac
      421 tgctgcggga tctgaggtca gcacacctcc aaggggactc caaccgctcc atcgggttcc
      481 gttccaggtt ctgatcatca gttcatttct ctgccctctt gcaaagactt caaggcggtt
      541 gcttccggta gatgccgcag ccggcccgga agttatctgc cctccgccaa gatcctccca
      601 agcgctccat cgggttccgt cccagtattt atgccaaagg gactgattct gccctctccc
      661 gaatacgtct attctgttcg gcccccagga tactgctgca ggtgctgaag tcagcactcc
      721 tcccagatcc tcccactcgc tccagcggat accgtcccaa tatttgtgcc acaaggattg
      781 attctgccct cgtgcaaata catccaatct atttgcctgc caggatgcag ctgcaggttc
      841 ggaagtaatg atgcctccaa gatcctccca tgcactccaa cggataccat cccaataaat
      901 atgccacaat gcctgattct gtccccttgc aaatacatct atcctatttg ttccccatga
      961 tacctctccc ggccctgttg tcagaatccc gcccaggtct tcccatgcac tccagcgggc
     1021 accgttccaa taaacatgcc acaatgcctg gttttgccct ctagcaaata catccagacg
     1081 gttaggctgc caggatgcag ctgcaggtgc ggaagtcagt attcccccaa ggtcttccca
     1141 ttggctccag cctgtacctt cagctacttc aatattttcc agcgtaaatt gaattatatc
     1201 cctcattatt tgatttatac gggcattagg aactcctttt cgtctaagta tggtgaaggc
     1261 atacggatta tcccgtcgaa gcgcattcag aagtgcattt gttctctgct ctatagtccc
     1321 attataattg tttgcatttc tcaatacata tattactgtg tttctaaata tgttacgcgt
     1381 atctgaacgc cgcaccccgt acctttcaag ctcttgatag tggccggcaa acctcctgtt
     1441 aataagcggc atgatgcggc tgacatcctg cggcctttgc ctcatacctt catagccata
     1501 ctcgtaatat tcatcctcaa agtacaattc atcgtaatac tcttcaatga aggagtcatc
     1561 atactctgta tcctccgtta aattttggaa cggacagatg taaaacggat tcatagggca
     1621 catataagga ttcttgtaca atttaattcc tatctccttt ctccacaaac gtatcattac
     1681 atgatatgca gcataactcc gaatggttaa aaggaaatgc agatagaaat gcactgtatc
     1741 cgttatgaag atacagtgca tttacttttt ttatcaatca ttttcctctc cggacgtatc
     1801 tctaattagc tatggcatct tcaaaccgta cataattcag cagcttgctt tccttataca
     1861 gccataagcc tacaaatcct tcatcaataa gttttctctg aagttcaaaa tcaccgatag
     1921 gtatgccaaa attctggctt acttttctta ccgcccttgg ctccagattt ctatgaagaa
     1981 taaacctcca cattccgtcc ttcccatctt tttctgaacc gtactttttc ttaagtgctt
     2041 tgattctcaa tccgtttttt ataatattgt aaaggctgta gatattctgg ggtgatacag
     2101 tacaaagcaa atgactgtac ccaaaatcct taatcattgg tatggcttgc tgcaatgtca
     2161 gactctgcaa tgagttgcct ctgtattcgg gatgcacaac tgtcgtttca agatgagcta
     2221 cctttgccaa ttcttgcctc ggcatattaa tatcattacc aagatttttt tcagagcatc
     2281 ccgggaaacc tatatatctg tatgcaataa tatctcctgt tttgttgaat actccgatta
     2341 tcttcgctcc ctgtttcata tcatcataca tttcttcata agaatccatg tacagaacct
     2401 gcttattagg aagctgctca tatacatatt tctggagtgc aactgcatct ccaagctcct
     2461 ccggagtaat tatcctcata acgtattcat cttgttcctt gctttcattt atatgcctgg
     2521 tcagttgtcc ttttttcaat atagtcatat gctagcctcc tttcaaaatt ctctgactgc
     2581 tgtacaagta gttacatcat ctgacaactt atgcaatttc tttaagttgt ctcttttatc
     2641 taatattatc acaggcaact cgtgtttgtc aagcaagttg tcagatattt cttaaaaaaa
     2701 atattgcttg caatgaaagc aaaaaaagcc tgatatgaaa ccatatcaga cttaagtttt
     2761 tactttacat atttagcaac tttttcagat atctccgcat cgcttcccac ataaagaata
     2821 atgtcattgg gaaagaacac catcttgggc cccggcgaaa gatacaggtg cccggaacga
     2881 tttattccta ttattgtcgc ccctgtgtga ttccagaaac gcacttcgcc gatggttttt
     2941 cccaccaagt ggcttttttc aggtattttt agctccaaag gaaatattat tccaatattt
     3001 ctaagaagca tcgcctgttc aataacggaa tccaatcgtt caagaacctg cttttcaagt
     3061 gcctgctttt cctccattaa tttgacaatc tcttcacgca tggaaagtat tctgtccttt
     3121 gattgatgtt cttttatgaa gttattggct gcatcttttg attttattaa tatacctttt
     3181 ttttctatta catttacaac ctgcagttct tccaacagct tcaatgcacg tcttattgtc
     3241 tccggagaaa cgctgtattt accggaaagt gtagacctcc ctttaaccat ttcgccgact
     3301 ttccattcct cattgagaat actgtaagct atatccagtg ctatcttctc atattttgca
     3361 gtcggtattt tatcttcccc atggcttttt agattcattt caacaccctc tgaattgtaa
     3421 atattggtat taattattcc ttactataat tcttgtattt tttacaccaa ttgtcaagca
     3481 tataatatga cctgatgttt tagttctgtt cctgcaccac agcgtctcgg tgcctggcgt
     3541 ccagattttt caattcccta ataagaaaaa ttcctgtaat gaccgacgat gtcaggtctg
     3601 acgcaggccc tgccataagc agtccttcca ggccgaaaaa taacggcagt ataagcagtg
     3661 ccggtatgag aatcaatact tgccgtgaaa ggctcaggaa tgccgcatgc ttcggtttgc
     3721 ccaccgcctg aaaataattt gccgatacaa tttgaaatcc gataatcgga agaaatatca
     3781 ggtagattga aatcgctctt gttccaaaat tcaataaatc cttatccgtt ctattgaata
     3841 ataatatcag ttgtgacgga aatactcttg tgagtataaa tccaatagtt gttataattg
     3901 ttgccgcaac aactgcaagt ttcaaagctt tcttcaccct gtcaaactca agtgccccgt
     3961 aattatatcc tataatgggc tgggcaccct gattgatacc aaatatcggc ataagtatca
     4021 gcatagcaat actcattata acacccattc ctgaaacggc tatatcgcct ccgtatatta
     4081 caaggctttt gttcatgatg gcattcagga cgcttgcggc catctgcatg aagaatgttg
     4141 cagctcccag ggaaacgatt ttaactacta tcggcgtatg cagcctgagg ttttttgtat
     4201 gtacctttaa ggtgcttctg cctccaaaga aatgatacaa cacccatgac gttgatacag
     4261 cctgtgacaa aacagttgca atagctgcac ctctaatgcc caatccgaat acaaagataa
     4321 atataggatc caatatggta ttcagaatcg ctccgattat catcgtaaac atagcaattc
     4381 tgggattccc ttctgccctg ataaagttgt tcatcccaaa gccgacagtc tgtataaccg
     4441 ttcctaaaag tattatgctt aaatattctt ttgcgtatgg aagtactgct gcgctggcgc
     4501 caaaaatcct taacagcggt tcaataaaaa tcagacctgt accggaaatt gtcatcgatg
     4561 taataacaag cattactact gcatttccta aaatcagctc cgcctcttcc ttcctctgct
     4621 ctccaagcct tatagaaatc agagagttcg ccccgattcc gataagcatt gcaaaggcca
     4681 taataacaag cataatcgga aagccgatgg ttatccctgc tattcccagg gggcctacgc
     4741 tattccctat aaaaatccga tccactacat tatataatgc atttaccagc atcccgacaa
     4801 tcgcaggaat tgaaaacttc agcagcagtt tggatatctt ttcttcgccc aattgtttag
     4861 atgtgtccat atattaactc ccactccaaa aataatatgt ctttaacaac aaccttcttt
     4921 tataatacag tatctccata atactaatga taagtttaaa aatcaagaaa aacactaaaa
     4981 tagagacatg gggacgggga cacggggacg tttcccctgt cccgctgaag cggggcacat
     5041 gcggctatag cgcaaaatat gaagaatgcg acgcattctt cgggtacgag gtacgaggcg
     5101 cgaggttcga g
//
LOCUS       DAOQUR010000098         5100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_18066_length_5100_cov_12.648365, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000098 DAOQUR010000000
VERSION     DAOQUR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18066_length_5100_cov_12.648365"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1174
                     /locus_tag="PKI50_11935"
     CDS             98..1174
                     /locus_tag="PKI50_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNT04450.1"
                     /translation="MNYVIIMCGGSGSRFWPKSRKMYPKQFLNTVGERTMIQLTVDRI
                     SKFIPLKNIYMVTNKNYVNTIREQVPEILEKNLILEPMIKETAACIGYSAVKLLKKDP
                     EAVMIVLPSDHYIQDEDIFIETLKQGLRIADSENCLVTMGIKPTRPETAYGYIETGRK
                     IEGSWEIPTYKIKRFTEKPNREKAQEFIDKGTFLWNSGMFIWKASVLLKQYKKFLPDM
                     YQCLKRISDYIGSPLEAKIVEEEYNKIDGISIDYGILEKTWDVYVMESSFYWDDIGNW
                     TAMERYMPKDECGNTLKGECKVLDSHNCILYGDKRLIAVLGVDDLVIIETDDVILVCR
                     KDRDQDIKLLIKELQEDKENMKYI"
     gene            complement(1198..2523)
                     /locus_tag="PKI50_11940"
     CDS             complement(1198..2523)
                     /locus_tag="PKI50_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT04451.1"
                     /translation="MTEGNVYRHIIVFAIPIIIGNIFQQLYNIVDAIMVGRYIGEDAF
                     AAIGVASPIMGTALFIIVGMCIGASVLMAQLFGANDQKKLKKEISTAFIAGSIFSIIV
                     SITFIIVTNSILRWLNTPDNLMHDARVYLITIFSGLIFTMLYNLFDAIFRSMGNSRIA
                     LFFLIIASILHIILDLVFIIKMNMGVFGVGLATVISQAASALLCAIYGLNTYPILRFK
                     KEEIMIDLKLLKKTAEFGWIAALQQSCVYIGKMLVQSAVNPLGISAIAAFSAVSRIDA
                     FFVTPGDGVASALTTFIAQNTGAGKKDRIKSGLKTGFSLLSVYGFSISLLVYFGAYPL
                     MGIFVENEGQSIISLGAQYLQVMSLFYILSGFGYAFQGFFRGIGKIGITFFATFFQII
                     IRIILSYSLSPVFGLAGVALAVGIGWLSLPIYHGIMYYRYIKKSRLLIE"
     gene            complement(2624..3766)
                     /locus_tag="PKI50_11945"
     CDS             complement(2624..3766)
                     /locus_tag="PKI50_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNT04452.1"
                     /translation="MNICMDARGAKLYAGTGIGTYTARLLENIIKVDTRNNYRFFWPN
                     DGYDFLLGRGNISLTLFGEKNKKFWDEVFLPAQVKMNSCDVFHLPQNGLGLPRPKTCS
                     YVATVHDLIPLVMPDTCGKSYLDKFLQEMPYILEKCDRVITVSNYSKQDIIKYFNLPE
                     EKIKVIYLAADSRFRLIDKEKAWDFLKKEYSYSNDFILYLGGFSPRKNVDGLLEAYRR
                     ICRELPGYYDLVLLGASKDSHYELKKKTEGMGIRDRVVFAGYVPYEHLPYFYNCSSLF
                     VYPSLYEGFGLPPLEAMTCGTPTITANVTSIPEVVGDGAILTDPYNIDELTEKIYKAI
                     SDAEFREQLKEKALRRAYNFSWKKTAIETIKVYEEAGGERREVRGL"
     gene            3887..4873
                     /locus_tag="PKI50_11950"
     CDS             3887..4873
                     /locus_tag="PKI50_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HNT04453.1"
                     /translation="MDLKAVEEAYGIKIIDIQSIRNILRAETDCGVKCIKRAHMSPGF
                     FLFIYSAVNHLKEKGYEGVIPYNRAIDGSICIPDGKHVYYVVDWIEARECRFRKEEEL
                     KMAIKAAAELHRASVGYVPPEGAKPRVFYNKWVEKCEKKSKELLEFGKAIEDKEHRDD
                     FDEIFALHLPYYLQQSLDTANMIMESPYWEISEKSEQSGEFCHHDMANHNFLITEEGK
                     VYLIDFDYCIMDTRLHDLTSLVIRNMRYGVWDIGKAYFILNEYDRIYGISRKELDVIK
                     ALITFPQDFWQVGLQYYTEKQPWTMDYFLMRLNRIADDREIRDKFLKEFYEL"
     gene            4886..>5100
                     /locus_tag="PKI50_11955"
     CDS             4886..>5100
                     /locus_tag="PKI50_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04454.1"
                     /translation="MSGRKNEINEAIDIERGLRPEEQVNKLESFIKDMKEEIKQLKKI
                     GKHKEKIEKLEREKKKIISKKLKKHHR"
BASE COUNT         1618 a          985 c         1112 g         1385 t
ORIGIN      
        1 tgtccccctg tcctctcccc gttacctatg tcgcattatt cgtctgtaac gaatcgttcc
       61 gtaccgcata ttataattat tacggaggtt tgtaattatg aactatgtaa taattatgtg
      121 cggcggttca ggcagcaggt tctggcccaa gagcaggaaa atgtacccca agcagttttt
      181 gaacaccgta ggagaaagaa ccatgataca gcttacagtg gataggatca gcaaatttat
      241 ccccctaaag aatatctata tggtaactaa caaaaactac gtcaacacca taagagagca
      301 ggtaccggaa atattagaaa agaatcttat attagaacca atgataaagg agactgccgc
      361 ctgcattgga tattcggcgg tgaagcttct caaaaaggat cccgaagcgg ttatgatagt
      421 acttccttcc gatcactata tacaggatga agatatcttt atagagaccc tgaaacaggg
      481 cctgcggata gctgattcag aaaattgcct ggtaaccatg ggtataaagc cgacaagacc
      541 ggaaacggct tacggttata ttgaaacagg aagaaaaata gaaggctcgt gggagattcc
      601 aacctacaag ataaaaagat ttacagaaaa gccaaacagg gaaaaggctc aggagtttat
      661 tgacaagggt acttttttgt ggaacagcgg aatgtttatc tggaaggcat cggtactgct
      721 taagcagtat aagaaatttc ttcccgatat gtatcaatgt ttgaagagaa tcagtgatta
      781 tataggaagc ccattggagg ctaagatagt tgaagaagaa tataataaaa tcgatggtat
      841 ttccattgac tatggaatac tggagaagac atgggatgta tatgtgatgg agagcagctt
      901 ttactgggat gacataggga attggactgc aatggaaaga tatatgccga aagacgagtg
      961 cggcaacacc cttaaggggg aatgcaaggt actggattcg cacaactgca tattgtacgg
     1021 ggacaaaagg cttattgcag ttctgggagt tgacgacctt gtgataatag agactgatga
     1081 tgtaatatta gtgtgcagaa aagacagaga tcaggatata aagcttctta ttaaggaatt
     1141 gcaagaggat aaggaaaaca tgaaatatat ataggggcac ttaatagcga agtgtcccta
     1201 ttcaattagc aatcttgatt tcttaatgta tctgtaatac attattccat gataaattgg
     1261 cagacttaac cagcctattc caactgccag tgcaacaccg gcaagaccga acaccggtga
     1321 aagtgaataa gacaaaatta ttcttataat aatctgaaaa aatgttgcga aaaatgtgat
     1381 accgatttta cctattccac ggaaaaatcc ttgaaaagca tagccaaatc ccgaaagaat
     1441 ataaaacaga gacattactt gtaagtattg cgctcctaaa gatataatgg attggccttc
     1501 attttctaca aatatgccca taagaggata cgccccaaaa tatactaaga gtgaaatgct
     1561 gaagccgtaa acagatagta atgaaaatcc ggtctttagt ccgctcttga ttctatcttt
     1621 ttttccggcg cctgtgtttt gagcgataaa ggttgttaaa gcgcttgcta cgccgtctcc
     1681 gggggtcaca aaaaatgcgt caattctgga tactgctgaa aatgcggcaa tggcgcttat
     1741 accaagggga ttgacagcgc tttggaccag catttttccg atataaacgc aagattgctg
     1801 cagagctgct atccaaccga attctgcagt ttttttcaac agtttgagat ctatcattat
     1861 ttcttctttt ttgaatctca aaataggata tgtatttaga ccatatattg cacacaataa
     1921 tgcgcttgct gcctgcgaaa ttactgttgc caatccgact ccaaagactc ccatattcat
     1981 tttaataatg aatactaagt ccagtattat atgtagaatt gatgctatta tcagaaaaaa
     2041 aagagctata cgggagtttc ccattgaacg aaatattgca tcaaacagat tatacaacat
     2101 cgtaaaaata agacctgaaa aaatagttat aagataaacc cttgcgtcat gcatcagatt
     2161 atcaggggtg tttaaccatc ttaaaattga atttgttaca attataaatg ttatgctgac
     2221 aataattgaa aatatacttc cggcaataaa tgcagttgaa atctcttttt ttagtttttt
     2281 ttggtcattt gcaccgaata gctgcgccat taaaacagat gctcctatac acattccaac
     2341 aatgatgaat agtgctgtgc ccattatcgg agaggcaacg cctattgcgg caaatgcatc
     2401 ctctcctatg tacctgccga ccataatcgc atctacaata ttgtaaagct gttgaaatat
     2461 atttccgatt ataataggaa tcgcaaaaac aattatatgt ctgtaaacat tgccttcagt
     2521 catgctgtgc ataatagttc ctcctcaggt ttgtcagttg tgtcaattat tttaattata
     2581 ctatacatct tccaaaaatt cttataataa gacgaaattt acactataaa ccccgtacct
     2641 cccgcctctc acctcctgcc tcttcatata cctttatggt ttcaattgcg gttttcttcc
     2701 atgaaaagtt gtaagccctc ctcaaagcct tttctttcag ctgctccctg aactctgcat
     2761 ctgaaatggc cttgtagatt ttttccgtaa gctcatcaat attgtaaggg tctgttagta
     2821 ttgctccgtc tcctaccact tcggggattg aagtcacatt tgcggtaatg gtgggagtac
     2881 cgcaggtcat tgcttcaaga ggcggaaggc cgaagccttc atacagtgaa ggatatacga
     2941 acagagagga gcagttgtaa aagtacggca agtgctcata aggcacataa ccggcaaata
     3001 ccaccctgtc ccttattccc attccctcag tctttttctt caattcatag tgactatcct
     3061 tggaggctcc cagcagcacc aggtcgtaat atcccggcag ctcccggcag attctcctgt
     3121 aggcctccaa taagccgtcc acattctttc tcgggctgaa gccccccagg tataatatga
     3181 agtcgttgga atagctgtac tccttcttta aaaaatccca tgccttttcc ttgtctataa
     3241 gcctgaacct gctgtctgcg gccagatata tgacctttat tttttcttca ggcagattga
     3301 agtactttat tatatcctgc tttgaatagt tggatactgt aattaccctg tcgcactttt
     3361 ccagtatgta gggcatttcc tgcagaaact tatccaaata gctctttccg caggtatcgg
     3421 gcattacaag gggtatgagg tcatgtacgg tagcaacgta agagcaggtt tttggcctgg
     3481 gaagcccaag gccgttctga ggcaggtgga atacatcgca gctgttcatt tttacctggg
     3541 cggggaggaa tacctcatcc cagaacttct tattcttttc gccaaacaaa gtgaggctga
     3601 tattgcccct tcccaataag aaatcatatc cgtcgttagg ccagaaaaac ctgtaattgt
     3661 tccgggtgtc aacttttatt atattctcca gcaatctcgc tgtatatgtg cctattcccg
     3721 ttccggcata gagcttggcg cctcttgcat ccatgcatat attcatataa ttcagctccc
     3781 tatatatcag tatattagaa cacccttgta cctgtgaata gtcaaagcag taacacaatt
     3841 tttgcggcgt catataatgg tataaatgct gttgagagga atcattatgg atttaaaagc
     3901 ggtagaagag gcctacggta ttaaaatcat agacatccaa agcatcagaa atatcctgag
     3961 ggcggagacg gattgcggag taaagtgcat taagagggcg catatgagcc cgggcttttt
     4021 ccttttcata tattcggcgg tgaatcacct taaggaaaag ggatatgaag gggtaatccc
     4081 gtataaccgg gccattgacg gaagcatatg catacctgac ggcaagcatg tctattatgt
     4141 ggtggattgg attgaagcca gggagtgcag gttcaggaag gaagaagagc tgaagatggc
     4201 aataaaggca gcggcggaac tccacagggc atcagtaggt tatgtgcccc cggagggagc
     4261 aaaaccaagg gtattctata acaaatgggt ggaaaagtgt gaaaagaaaa gcaaggaact
     4321 gctggaattc ggcaaggcaa tagaagataa ggagcatagg gacgactttg acgaaatatt
     4381 tgcccttcat ctcccgtatt atttgcagca atccctggat acagcaaata tgataatgga
     4441 aagcccatat tgggaaatat cggagaaaag tgagcagtca ggggaattct gccatcacga
     4501 tatggcaaac cataacttcc tgataactga ggagggcaag gtttacctta ttgacttcga
     4561 ttactgcata atggatacca ggctgcatga ccttacaagc ctggtaataa ggaatatgag
     4621 atacggagtc tgggacatag gcaaggcata tttcatactg aacgaatatg acaggatata
     4681 cggcataagc cggaaggagc ttgatgtaat aaaggcatta ataacctttc cgcaggattt
     4741 ttggcaggta gggctccagt attataccga aaagcagccc tggaccatgg attattttct
     4801 gatgaggctt aacaggatcg cagatgacag ggagattagg gataagtttt taaaggaatt
     4861 ctatgagttg taaggagttg attttgtgag cggcagaaag aatgaaatca atgaagcaat
     4921 agatattgaa agaggcctga ggcccgaaga gcaggtcaat aagctggaaa gcttcataaa
     4981 ggatatgaag gaagaaataa agcagcttaa aaagataggc aagcacaagg aaaaaataga
     5041 aaagcttgaa agggaaaaaa agaaaattat cagcaagaag ctgaaaaagc atcacaggca
//
LOCUS       DAOQUR010000099         4910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_19075_length_4910_cov_10.130587, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000099 DAOQUR010000000
VERSION     DAOQUR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19075_length_4910_cov_10.130587"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="PKI50_11960"
     CDS             <1..1722
                     /locus_tag="PKI50_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HNT04455.1"
                     /translation="FELLEDEAQSAPKQSNAQRQDSSGQQNSTQNQRGKSSRHPLTVE
                     EWNGGRKAENTEDVCQAPNNTNIMSTKLCENLEAIKQKFLMPKNRDIIVREFRIGRKV
                     KAFMVYIDGMMDKQTLNLSILPQLMSRDVFEGLGEECPVDYLMENILAVHDVRKTNKY
                     SDTVMQILNGLSALFVEGCGECIIIETRGYEKRNVDTPKTETVVKGSQEGFTENLRTN
                     VALIRKIIKNENLVTEMLSVGNTNHSNCAIMYLEGITNPKVIQEVKKRLERIDTDFVL
                     GDGMIEQFIEDNSFMLFPQIASTERPDRTASFIMEGRVVIIAEGTPFALSAPVTFFGL
                     FHTSEDTFVRWPGGTFLRLIRIFGLFCATFLPGLYVALTLYHIEMIPTELLISIAKAK
                     ELVPFPTLLEVLIMEIAFELIREGGIRVPSVIGQTLGIVGALILGQAAVTAGLVSPLL
                     VIVVSITGLGSFAIPNYTLAIAIRIERFLFIFAGAVLGFYGISLMAVLLSYSACSMKS
                     FGVPYFVPVAPKTKVNPDIILRYPIWTQKERPDALNTPDRKRQGDNPKLWISEDNPDE
                     TDKGGNT"
     gene            1719..>2728
                     /locus_tag="PKI50_11965"
     CDS             1719..>2728
                     /locus_tag="PKI50_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HNT04456.1"
                     /translation="MIKEGKFGVYEAVCLVIIAICNKIFSTTPGYVTRTIGTTGWYMT
                     LISCTTAIAFFALIYLLLKRFPGKNIVEIYDASLGRVVGFVFSFLLMMSFLSSVGIYS
                     REFTEIMKTYALPATPPSVIIGTLIFTASVAAFLGLETIARVAKLALYPALLGYFLIL
                     LFSSEYFELHNLFPILGYGLDKTVFTGILRASAYDEVTILAVFAGSLQGTAHIKKAGF
                     ISLLLSGFIISLGLICFSLAFGYTSIQEITIVAYILTRGLKYGTFFQRLDPIFLLLWI
                     ITTTIYISILFYTTVSIYCKLFRLQDARPVAIPMAVLAVSAAIIPKDFSSVLSVYVEG
                     IRT"
     assembly_gap    2729..2828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2893..4152
                     /locus_tag="PKI50_11970"
     CDS             2893..4152
                     /locus_tag="PKI50_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HNT04457.1"
                     /translation="MRRLVSVVLLFVSIISLLTACWDAREIDEWSYVYIIGVDKGVGN
                     TMRFTFQLPSLKHEGTGAGEQLGTTGQYTTSIDAPTMYAGVNIAESFTSRRLNYMHTK
                     YIVISEDLAREGVERFINGMVRSRQIRRIAHIIIVKGKASRFVEEFKPLLTSAVSKAQ
                     EGFMDMHWDTGLFIDTSYQDFFGDLKSTTKMPAVPLAAFNDFSNYRTSGNPPKEFKSE
                     GDYYAGELPRSGGNKFEFFGTALFDGDKMVGELNGDESRALLMLRGEFNSGSIAMADP
                     KDKELRITIDTKTQKKPDIKINFLEGKPVVNVKIFLEGDIQNLQSSEEYESVELKPVL
                     EEAFKVYIKGIIDKTIDKCKTLNVDVFGFGEKAAMHFLTIQEWEEYNWLSRFKDAEVN
                     TEVEFTIRRTGTLLKTNPTRYSEGEKK"
     gene            4149..4310
                     /locus_tag="PKI50_11975"
     CDS             4149..4310
                     /locus_tag="PKI50_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04458.1"
                     /translation="MTYIVILFLIILSIYPLSYAKYNWDRENKLGAIGTIILAMAAIA
                     VPSILLLFR"
     gene            4410..4486
                     /locus_tag="PKI50_11980"
     tRNA            4410..4486
                     /locus_tag="PKI50_11980"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4444..4446,aa:Arg,seq:tcg)
     gene            complement(4576..>4910)
                     /locus_tag="PKI50_11985"
     CDS             complement(4576..>4910)
                     /locus_tag="PKI50_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04459.1"
                     /translation="IGSLLITGGFILLLLSVFEVSKKLLGIASILLGVLLLIAAIAIL
                     ILLGLLLITPYVLLFLGIALIILGVILLLLHGSEPKKNNQDKNKHIYERDREICVKKK
                     KDSEFINW"
BASE COUNT         1376 a          948 c         1118 g         1368 t
ORIGIN      
        1 tttgagcttt tggaagatga agcacaaagc gctccaaagc aaagcaatgc ccagcggcaa
       61 gacagcagcg ggcagcagaa cagtacgcaa aaccagaggg gtaaatcctc caggcacccc
      121 ttaacagttg aagaatggaa tgggggccgt aaggctgaaa atactgaaga tgtctgccaa
      181 gccccgaaca atacgaatat tatgtcaacc aaattgtgtg agaaccttga agcaatcaaa
      241 caaaaatttc tcatgccgaa aaaccgggat ataatcgtcc gcgagtttag aatcggcaga
      301 aaagtcaaag cttttatggt ttatattgat ggtatgatgg ataagcagac cctgaacctt
      361 tccatattgc cgcagctgat gtccagggac gtttttgaag ggcttgggga agaatgtcct
      421 gtagattatt taatggaaaa tatcctggca gtccatgatg taagaaaaac aaacaagtac
      481 agcgatactg tcatgcagat cctgaacggc ttgagtgcct tattcgtgga aggttgcggt
      541 gagtgcatca ttattgaaac caggggatat gaaaaaagaa acgtggatac acccaaaacc
      601 gaaacggtgg taaaaggttc acaggagggt ttcacagaaa acctccgtac caatgtggca
      661 ctgataagaa agatcattaa aaatgaaaac cttgtgactg aaatgctgtc tgtcggtaat
      721 acaaaccatt caaactgtgc catcatgtat ctggagggta tcacgaatcc taaggtcata
      781 caggaggtaa aaaagcgcct tgagaggata gacactgatt ttgtcctggg tgacggtatg
      841 attgaacagt tcatagagga taattccttt atgttgtttc cccagattgc ttcgactgaa
      901 aggcctgaca ggacagcatc cttcattatg gaaggtcggg ttgtcattat tgctgaaggc
      961 acaccttttg cgttatcggc tccggtgact tttttcggtt tgtttcatac ctcggaggat
     1021 acttttgtcc ggtggccggg gggtaccttc ttaagactga taaggatatt cggccttttt
     1081 tgtgctacct ttctgcccgg gttgtatgtg gctctcactc tttatcatat cgaaatgatc
     1141 ccgacagagc ttttgatttc tattgccaag gcaaaggagc tggtgccttt tccgacactt
     1201 ctggaggtac tgataatgga gatagccttt gagcttatac gtgaaggcgg gatacgtgtg
     1261 ccaagtgtaa tagggcagac cctcggcatt gtcggtgctt tgatactggg gcaggcagct
     1321 gtgactgccg ggctggtaag tccgttgctg gtaattgttg tatccatcac agggttgggc
     1381 agctttgcca tacccaatta cactcttgca attgccatca ggattgaaag gtttcttttt
     1441 atatttgcag gagctgttct gggcttctac ggcatatcct taatggctgt actgctttcg
     1501 tattccgcct gcagcatgaa atccttcgga gtcccgtatt ttgttcctgt tgctccaaaa
     1561 acaaaagtaa atcctgacat tattcttaga tatcccatat ggacacaaaa ggagcgtcct
     1621 gatgcactca acactccaga cagaaaacgc cagggagata atcccaagct ctggatttcg
     1681 gaggataacc cggatgagac agacaagggg ggcaacacat gataaaggaa ggcaaattcg
     1741 gtgtttacga agcggtatgc cttgtaatca tagccatatg caataagata ttttctacaa
     1801 cccccggtta cgtaacgagg accatcggta caaccggctg gtacatgaca ttgatttcct
     1861 gtacaacagc tattgcgttt tttgctctta tctatctgct gctgaaacgt tttcccggca
     1921 aaaatattgt tgaaatatac gatgcttctt taggcagggt ggtaggattc gtattctctt
     1981 ttttacttat gatgtcattt ttatcaagtg tgggcattta ttcgagagaa tttacggaaa
     2041 tcatgaagac atatgcatta ccggctactc ctccgagcgt gataataggg acattgattt
     2101 ttacagcttc agtagcagcg tttttggggc ttgaaacaat tgcaagagtg gcaaagcttg
     2161 cgttatatcc ggcactcctg ggatatttcc tgatactgct tttttcatcg gaatattttg
     2221 aattgcacaa cctttttccc attttgggat atgggcttga caaaacagtt tttaccggca
     2281 tattgagagc ctctgcctat gatgaagtga ctatccttgc tgtttttgca ggctcacttc
     2341 aaggaaccgc ccacataaaa aaagccgggt tcatttccct gcttctgtcg ggatttatta
     2401 taagcctcgg gctcatatgc ttctcccttg catttggata tacaagcatt caggaaatta
     2461 cgatagttgc atatatcctg acgagaggct tgaaatacgg aacttttttt caaaggctgg
     2521 atccgatatt tttgctcctc tggattatta ctacaactat atatatttcc attttgtttt
     2581 acactactgt gagcatttac tgcaaattat tcagattgca ggatgcaagg cctgtggcta
     2641 tcccgatggc tgtacttgct gtttccgctg caattattcc gaaggatttt tccagcgttc
     2701 tttcagtata tgtcgaggga ataaggacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnntt tttatcatgc ccgcaatggc gcttatcgtt gcagtgatac gtaaaaagaa
     2881 gggggaacct gaatgcgcag attagtatct gttgtactat tgtttgtttc tataatatcc
     2941 ttgctgactg catgctggga tgccagagaa atcgatgaat ggtcgtatgt atatataatc
     3001 ggcgtggata agggtgttgg taatacaatg cgctttacct tccagctgcc gtctttgaag
     3061 catgagggca caggggccgg agagcaatta ggtacaacag gccagtatac tacgtccata
     3121 gatgccccta ctatgtatgc aggagtgaat attgcggaat cattcacatc cagaaggctg
     3181 aattacatgc ataccaagta tattgtaata tctgaagact tagccaggga aggcgtggag
     3241 aggtttataa acggtatggt caggagcagg cagataagaa ggattgcgca tataattatt
     3301 gtaaagggca aagcaagcag gtttgttgaa gaattcaagc cgttgctgac atcagccgtt
     3361 tcaaaggctc aggagggttt catggatatg cattgggaca ccggcctttt tatagatacc
     3421 agctaccagg attttttcgg ggatttgaag tctaccacca aaatgcctgc tgtgcccctt
     3481 gcggcattta atgatttttc aaattacaga acaagcggca atcctcctaa agagttcaaa
     3541 tctgaagggg attattatgc cggagagctt ccgcggtcag gggggaataa gtttgaattc
     3601 tttggtacag ccctgtttga cggggacaaa atggtaggtg aattaaacgg cgatgagtca
     3661 cgtgccctgc tgatgttaag aggtgaattc aacagcggct caatagccat ggctgacccg
     3721 aaagacaaag agcttagaat aactattgac acaaagacac agaagaaacc ggatataaaa
     3781 attaattttc ttgaggggaa gcctgttgtc aatgtaaaaa tattcctcga aggcgatatt
     3841 caaaaccttc aaagctctga agagtatgaa agcgtagaat taaaacctgt tttggaagaa
     3901 gctttcaagg tatatatcaa gggtattatt gataaaacta ttgataaatg caaaaccctt
     3961 aatgttgatg tattcggctt tggtgaaaaa gcagccatgc atttcctgac tatacaggaa
     4021 tgggaagaat ataactggct gagccgcttc aaggatgcgg aggtcaacac cgaagtagag
     4081 tttaccataa gacggacggg gacattgctg aagacaaatc ctaccaggta ttcagaggga
     4141 gagaagaaat gacatatatc gttatacttt ttttaataat attgtcaata tatccgctta
     4201 gctatgcaaa atataactgg gacagggaaa acaagctggg tgcgataggg acaattattt
     4261 tagccatggc ggcaattgca gttccttcca tacttttact gtttagatag ttatgaatat
     4321 ataaaatcaa agctttgcat aaaactgcct gtaaattaat gaccttgact atcataccgc
     4381 attttcatat aatgtttgtg tgacttaatg cgcccatagc tcagctggat agagtggctg
     4441 acttcgaatc agtaggccgg gggttcgact ccctctgggc gcaccattgt ctattttttt
     4501 gcatagttgg attcgaaccc gcgagggaga cccccaagtt aacttaaaag attcatatgg
     4561 ttaggcttgt cccatttacc aatttataaa ttcactatct ttctttttct tgacacatat
     4621 ttctctgtct ctttcatata tatgcttgtt tttgtcctga ttattcttct tgggttccga
     4681 gccatgaagc agaagcagta tcactccaag gataatcaac gcaattccta agaacagcag
     4741 cacatatggg gtaatcagca gcagtcccaa taggatcaat atggcgatag ctgcgatgag
     4801 caacaatact cccagcaaga tggatgctat tcccaaaagc tttttgctga cttcaaagac
     4861 tgaaagcaaa agcagtataa aacctccggt gatgagcaac gaaccaatga
//
LOCUS       DAOQUR010000100         4850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_19394_length_4850_cov_11.845881, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000100 DAOQUR010000000
VERSION     DAOQUR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19394_length_4850_cov_11.845881"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1490
                     /locus_tag="PKI50_11990"
     CDS             171..1490
                     /locus_tag="PKI50_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT04460.1"
                     /translation="MKIKKSDIYYLAIFLALAIIPVMYTIKLYDRGIPADSFEGEKET
                     IELRFLSSWGGTDTKAQQLQMLLDEFEEQNPGVKVINESMSGSEFLFKLKTNFAQGSD
                     PDVFGLWPGSDIKILINQGKVADLTELLASDPEWAAGFGEDAWSYDRFNGRIYGLPCE
                     IIYEGLFVNRDLFEKYGIKVPETYEELKDAVRAFRQRDIIPIAYNSTPEGTFLYQNIV
                     MKLGGREYTENPYKEGRINENYIEGMKYVKELYELGAFPEDAFVIADKTRDNLFLEKE
                     AAMIVQGSWFIGLGSLGAEDETVDIIPFPAFKEGKSHTSSIIYGLGNGNFHMSRKAYE
                     NPDKREMCIKLLKHLTSVEAAKLFSHETGFISNIKIPDQSVQPGRLMEKGKELIARSM
                     ELVGPTDSFIDRNLWEQVLVTSFPHVLEGRMTPEEVFEEMERRAAQQ"
     gene            1503..3317
                     /locus_tag="PKI50_11995"
     CDS             1503..3317
                     /locus_tag="PKI50_11995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNT04461.1"
                     /translation="MESIINKFVLFYKNTSIKNKLLLLFYIQIVIPLFFIGITSYQKS
                     AEIIEQKSINYSLDILKMIELRFKDLSTGMQGLTLELLYDNRVYDALSSKGYEDNITI
                     YNRANEIRSILRQATLSRNEIQSICLVTKNRRFYSFDSDNGKVRIEFILPYDSIVEAA
                     RKGRGRPVWFLEKQEGRESEIYVTRMVYDRDSYNEIGLIAINIKKEYLESVYTDLSRE
                     SLNNISILSENNEEIIKQQENSGILKNFYRQQLQGRRGFYKDNKAGMLVSYVLLEDPS
                     WKIVYHIPLKELYREMDTLKRWVLLIIVYGLIILSVLSVLTSVDIITPINKLVEGMKK
                     VEKGNKHEDIELDRSDELGYLSESFNRMSKKIDYLVNRIYKEEIALKEAEIKALQAQI
                     NPHFLFNTLENINWMARLNGVPEISETVSALAKLIDGSIGRGDRTISLKEELEYIDNY
                     MTVLKNRYEDRLEVIKILDEGLMDKKIPRLLIQPLVENAVKHGIGKSRRKGVIRLEAF
                     REEGHIVFEVEDNGMGMTAEELEALNKRLQEDELILEGNGTAPARKSIGLENVNRRIK
                     LLYGSSYGVKIESSYDEYTKVTVRIPDEQVQEGDKGNV"
     gene            3280..>4850
                     /locus_tag="PKI50_12000"
     CDS             3280..>4850
                     /locus_tag="PKI50_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT04462.1"
                     /translation="MNRFRKETRAMYKVLIVDDESIIRKGLKNIIDWKSLDCEVCGEA
                     GDGMEGIRMIRELRPHILITDINMPEIDGLSMIKETKQLLPGSKIIILTGYRDFEYIQ
                     EAIRLGAFDYLLKPSKIEDLTAIVQKAIAELQRSCKMEEELNNLKKHFKSKIPVLRQK
                     LLYDIIFRINTKKYEILEELNLYGLTIDKFKIMAVETDEEGQENAKSQYEKHLYQFGI
                     INTFEEILGTSFKTADVPINSRTILFVIQPNEESEGDMFSSLYKDAVSLQELVRNCFS
                     FTVTISISSEGNGALELADKAREALEALDYKFYMGKNTIILYEDLKSFYKSADYSALE
                     VYEKSLVQMVKSGNKDSVSLVMKDILKCVNECKLDRESIKKFYWNLINLVCSIPTTMK
                     LNDESFHYRRITEIFAALDQCESIIEINGILEEAALNIAERINSFNRKTINSTLQKAM
                     DYICLNYNEPITLNEVAEHTYVSIYYLSRMFTKELGKNYVDYLNEVRIEKAKEYLKDS
                     NCKTYEIAELVGIKDA"
BASE COUNT         1587 a          853 c         1239 g         1171 t
ORIGIN      
        1 tgtccccatg tctcattgct atgacgcatt cttcatatag acccttatgg agggtctttt
       61 tttgttctgt tattgtaata tattgcacta tgacaaaatt ttgcaggaaa tttacgtaaa
      121 tttagagaat tagtactcaa tttgtgaatg cacattttgg ggtgaaatat atgaaaataa
      181 aaaagtccga catatattac ctggcaatat ttcttgcgtt ggcaattatt cctgtcatgt
      241 atacaattaa gctgtatgac aggggtattc cggccgacag cttcgagggt gagaaggaaa
      301 caattgaatt aagattcctc agcagctggg ggggaaccga tacgaaagcc cagcagcttc
      361 agatgctgct ggatgaattt gaggagcaaa acccgggagt aaaggtaatc aacgaatcca
      421 tgtccggttc ggaattcctt ttcaagctga aaaccaattt tgcacagggt agcgaccccg
      481 atgtattcgg gctttggccc ggctcggata ttaagatatt gataaaccaa gggaaggtgg
      541 ctgacctgac tgagcttctg gcttcagacc cggaatgggc cgccggcttc ggtgaagacg
      601 catggagcta tgaccggttt aacggcagga tctatggctt gccctgcgaa atcatatatg
      661 aggggctgtt tgtgaacagg gacctttttg aaaagtacgg aataaaggtg ccggagacct
      721 atgaagagct taaggatgcc gtaagggcat tcagacagag ggatataatc cctattgcat
      781 acaattccac accggaaggg acatttttgt atcaaaatat agtaatgaag cttgggggca
      841 gggaatatac cgagaacccc tataaggaag gcaggataaa cgaaaactat attgaaggaa
      901 tgaaatatgt taaggagctg tatgagctgg gggcatttcc cgaagatgct tttgtgattg
      961 cggataagac cagggataat ctctttcttg aaaaggaagc cgctatgata gttcaaggct
     1021 cctggttcat cggcctcggt tctctgggcg cagaggatga aacggtagat attatacctt
     1081 ttccggcttt taaggaaggg aagtcacaca caagcagcat aatatacggt ttgggaaacg
     1141 gcaatttcca tatgagcaga aaagcctatg agaatccgga taaaagagag atgtgcatca
     1201 agctgctcaa acaccttaca tcagtggagg cagcgaagct gttcagccat gaaaccggct
     1261 tcataagcaa tataaagata cctgatcaga gtgtacaacc gggcaggctt atggaaaaag
     1321 gcaaggaact gatagcccgg tcaatggaat tggtagggcc tacagacagc tttatagaca
     1381 gaaatctgtg ggagcaggtg cttgtaacaa gctttcccca tgtcttggag ggaaggatga
     1441 caccggagga agtttttgaa gaaatggaac gcagggcggc acagcagtaa gggagatttg
     1501 caatggaaag tattattaac aaattcgtgc tgttttacaa gaatacatct ataaagaaca
     1561 agctgctgct gttgttttat atacaaatag tcataccact gttctttatc ggtattactt
     1621 cttatcagaa gtctgctgaa ataattgagc agaagtctat taattattct ctggatattc
     1681 tgaaaatgat agaactgcgc tttaaggacc tgtcaaccgg tatgcagggg cttacccttg
     1741 aactgcttta tgacaacagg gtttatgatg cactgagcag caaagggtat gaggataata
     1801 taaccatata caacagggca aatgaaataa gaagcatatt aaggcaggca accctttcca
     1861 gaaatgagat acaatcaata tgccttgtga caaagaacag gaggttttac agctttgatt
     1921 ctgacaatgg gaaggtaagg atagaattca tacttcctta cgacagcatt gtagaagcag
     1981 cgcgaaaagg caggggcagg ccggtatggt ttcttgaaaa acaggagggc cgggaaagcg
     2041 aaatatatgt tacccgaatg gtttatgaca gggacagcta taatgaaatc ggcctcattg
     2101 ccataaatat taaaaaggag tatcttgagt cggtatatac cgatttatcc agggagtccc
     2161 tgaacaacat ttcaatactc tcggagaata atgaggaaat aataaagcag caggagaact
     2221 ccggaatact taagaacttc taccggcagc agcttcaggg gagaaggggc ttctataagg
     2281 ataacaaggc aggaatgctt gtatcctacg ttctgcttga ggatcccagc tggaagattg
     2341 tatatcatat accccttaag gagctttatc gggaaatgga taccttgaag agatgggtgc
     2401 tgctcattat tgtatacggg cttattattc tatcggtgct cagcgtgctt acttcggtgg
     2461 atataattac tcccatcaac aagctcgtag agggaatgaa aaaggtggag aagggcaata
     2521 agcacgagga tattgaactt gacaggagtg atgaattggg gtatctgagt gagagcttca
     2581 atagaatgtc aaaaaagata gattacctcg ttaacaggat atataaagaa gaaatcgctc
     2641 ttaaggaggc tgagataaag gcgctgcaag cccagataaa tcctcacttt cttttcaata
     2701 ctcttgaaaa tattaattgg atggcccggc ttaacggagt gcctgagatc agtgaaacgg
     2761 tatccgctct tgcaaagctt atagacggaa gcataggcag gggggacagg accatatccc
     2821 tgaaagaaga actggaatac atagacaact atatgacggt attgaagaac agatatgaag
     2881 ataggcttga agtaataaaa atcctggatg aagggcttat ggacaagaaa ataccaaggc
     2941 tgctgattca gcccctggtt gaaaatgctg taaagcacgg gatcgggaaa agccggagaa
     3001 aaggagtaat caggcttgag gcattcaggg aggaagggca tatcgtattt gaggttgagg
     3061 acaacggaat gggaatgacg gctgaggaac tggaagccct gaacaaaagg ctgcaggagg
     3121 atgaattgat actggagggg aacggtacag ccccggcaag aaaaagtata ggcctggaaa
     3181 acgtgaacag gaggataaag ctgttgtacg gaagctcata cggagtgaaa atagaaagca
     3241 gctatgatga atataccaag gtcactgtaa ggattcctga tgaacaggtt caggaaggag
     3301 acaagggcaa tgtataaggt gctgattgtt gatgatgaat ctatcataag aaaaggcctc
     3361 aaaaatataa ttgattggaa gagcttggac tgcgaggtat gcggggaagc cggcgacgga
     3421 atggaaggca tcaggatgat aagggagctg cgtccccata ttctcataac tgatattaat
     3481 atgcctgaaa tagacggcct ctctatgatt aaagagacca agcagctgct tcccgggagc
     3541 aagataataa tacttaccgg ctacagggat tttgaataca tacaggaagc tataaggctg
     3601 ggggcctttg attatttgtt gaagccgtca aaaatagagg atttgaccgc catagttcaa
     3661 aaggcgatag cggagctgca gcgcagctgt aagatggagg aagaactcaa taacctgaag
     3721 aagcatttta agagtaaaat accggtactc aggcagaagc tgctgtacga tattattttc
     3781 aggataaata caaaaaagta tgagattttg gaggaactta atctttacgg gcttactatt
     3841 gataaattca aaatcatggc tgtggaaacc gacgaagagg ggcaggaaaa tgcgaaaagc
     3901 cagtatgaaa agcatctgta ccagttcggt atcatcaaca cctttgagga aatactcgga
     3961 acaagcttca agacagcaga cgtgcctata aacagcagga ccatattatt cgtaattcag
     4021 cccaatgaag aatccgaagg agacatgttc agctctctct acaaggatgc tgtgagcctt
     4081 caggagctgg tgcgcaactg cttcagcttt accgtcacaa tatccataag ctcggagggg
     4141 aacggggccc ttgagcttgc agataaggca agagaggccc ttgaagccct ggattataag
     4201 ttttatatgg gcaagaatac aataatactg tatgaagatt tgaaatcctt ttataagtct
     4261 gctgattatt cggccctgga ggtatatgaa aaatctcttg ttcaaatggt caagtcaggc
     4321 aacaaggaca gtgtgtccct ggtgatgaag gatattttaa aatgcgtgaa cgagtgcaag
     4381 cttgacaggg aaagcataaa gaagttctat tggaatctaa taaaccttgt ctgcagcata
     4441 cctacaacaa tgaagctgaa tgatgagagc tttcattata gaagaattac agaaatattt
     4501 gccgcccttg atcagtgcga aagtatcatt gaaataaacg gaatattgga ggaagctgca
     4561 ttaaatatag cagagcggat aaacagcttc aacaggaaga caataaattc cacactgcaa
     4621 aaggctatgg actatatctg tctgaactat aacgagccca tcacactgaa tgaagttgcg
     4681 gagcacacct atgtgagcat atactatctg agcaggatgt tcacaaagga attgggaaag
     4741 aactatgtgg actacctgaa tgaggtaagg atagaaaagg cgaaggagta cctgaaggac
     4801 tccaactgca agacgtatga aatagcagag cttgtgggga tcaaagatgc
//
LOCUS       DAOQUR010000101         4802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_19650_length_4802_cov_8.633663, whole genome shotgun sequence.
ACCESSION   DAOQUR010000101 DAOQUR010000000
VERSION     DAOQUR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19650_length_4802_cov_8.633663"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="PKI50_12005"
     CDS             complement(<1..1794)
                     /locus_tag="PKI50_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04463.1"
                     /translation="MKRLILTLSVIIALLSGCTVKQGQLVVIENESPNEIRQDKSVYE
                     HLNVESRIFPIEKYKEAIGKIEAGMIPLAVSPDYSMVVAYQPTGSPVPDITNRRVVIG
                     NMVQEINLYICSTENGESKSLGKFLSLKDFHFDETGNSLAFIDGNSNIYIYSSITGQL
                     QKLVTAEKRDAYNILSWSKDSKRLMINTRMEFDIASREFISIAVNSYTPFIRSKFIDS
                     NYIVQMKNNEYNDMIAFYDFSTKEFTSIANGIYTDSDNSNVLYTKDYMSGLNIVSLKT
                     LESKSIENGPVYCSYIMKSTGDILYSTINNDLDSSYRYLLVKVNPHTMAKTSVKLDSP
                     TFHMSPAEDKLYVISSHSSNSIEIDAKELKVHRNESMKDDPDLYGIKTTLLKMFQLDY
                     NFNEAYEKYENMAKEIYINTYDPLPQEALENKLVDFKRYNMPIPTNQREDHIPPTIYF
                     DQLNITDSKASINLGLYYINSVEMVKKDGSWYITGFSTHPASGEIASVTSIVKKHLYN
                     IKNKNINEAVKYWTAEEDTEYLAAQRKIVEELIDRADELTFEVGEIDLWSMSDPHRAE
                     SPDRATHAKVKILINDGNKTTKYKLILSRQYK"
     gene            complement(1809..3215)
                     /locus_tag="PKI50_12010"
     CDS             complement(1809..3215)
                     /locus_tag="PKI50_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT04464.1"
                     /translation="MKLKHKIIALNMGALFFMLIILGTIITAITDNYNLSTTLKYLEN
                     QGNYSAIYIEQYALSKAPNDFEVPSVMDASANYLAAVLNDLVKCRVQIYYGTELLGDS
                     EESTGFNGEIRPEIQETFKMKKAYFVAAGKNRTFYYAVPVSISNRYTYSLGFIYDLSE
                     VDNMKNNTVRMFVFTGVFLSIFMMLGSTIISNRITQPIKMLNEATRQFSEGNFESRAC
                     TATRDEVGELSVTFNSMADSIEDMINKLNHEKEKQKYFFDNFTHEIRTPLTTILGYAD
                     LLWKTNDENVKDKSLFYITSEGKRMLKMMERLLELSKLKNYNFELNKSDSNLKKLIED
                     ACDSMHYKMKRYNTSCRLDLEDITWKVDPDFFKQVIINLIDNSIKYSKSPVIDISLRK
                     KDKVVLEVVDYGCGIDIKNLESLFEPYYKVEKSRNSHNEGWGLGLSIVKEIIDKHGGS
                     IDIRSTAGKGTSIIITLE"
     gene            complement(3212..3883)
                     /locus_tag="PKI50_12015"
     CDS             complement(3212..3883)
                     /locus_tag="PKI50_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT04465.1"
                     /translation="MKVLIVDDEPAIVDLIKINLELEGFDTCVCYTGKQAVKAVPIFM
                     PDLILLDIMLPDINGFEVCKKLQDLSIPIIMLTAKNDIKDKLYGLELGADDYITKPFD
                     SRELTARIRTVLKRINKSEPKDDPNIQLGPIMINTSERQVYVDEEEIVLTPKEYDLLL
                     VLCENQRKVFSRENLLELVWKFEYIGDSRTVDMHVQRLRKKLGKYSFFIKTVFGIGYK
                     FEVQV"
     gene            complement(4007..>4802)
                     /locus_tag="PKI50_12020"
     CDS             complement(4007..>4802)
                     /locus_tag="PKI50_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HNT04466.1"
                     /translation="GTPAEETDGAKVDMAEKGVFDGIDAAMILHPEDKTYESGSSLAM
                     DAIQFDFRGKSSHAASSPHEGINALDAVILTFNGINALRQHVTSDVRIHGIIAEGGRA
                     ANVVPSKAVAQFYVRAGKKDYLKEVTEKVKNIARGAALMTGAALEISNYEISYDDMNT
                     NINMSKTFTENLRLAGVGEINAERFSFGSLDMGNVSHVVPAIHPYISISDTDLIGHTT
                     EFRDATLTDRAHDALVKGACALALTGYDILTDKELLERIKKEFSLK"
BASE COUNT         1187 a         1070 c          939 g         1606 t
ORIGIN      
        1 cttatactgt ctcgagagta taagcttgta ttttgtggtt ttgtttccgt cgtttatcag
       61 tatttttacc tttgcatggg tggctctgtc cgggctctcc gccctgtgag ggtcactcat
      121 ggaccaaaga tcaatctccc ctacttcaaa ggtaagctca tcagctctgt ctataagctc
      181 ctcgacaatt tttctctggg cagccaggta ttctgtatcc tcttcggcgg tccaatactt
      241 cactgcttca ttaatgttct tgttctttat attgtaaaga tgcttcttaa caattgaggt
      301 taccgaggct atttctccgg aggcagggtg ggtcgaaaaa cctgttatat accagcttcc
      361 gtctttcttt accatttcca ccgagttgat ataatacagg ccgaggttta tcgatgcttt
      421 ggaatctgtt atattaagct ggtcaaaata tattgtcgga ggtatgtgat cctcacgctg
      481 attggtcggt atgggcatgt tgtacctttt gaagtcaaca agcttgtttt ccagcgcttc
      541 ctgaggaagg ggatcataag tattaatata aatttccttt gccatattct catacttttc
      601 ataggcttcg ttgaaattat aatccaattg aaacattttc agcagggtgg tcttgattcc
      661 gtacaaatcc ggatcatcct tcatgctttc attcctatga accttcagtt cttttgcatc
      721 aatttcgatg ctgtttgaac tgtggctgct tataacatac agcttatctt ccgcggggct
      781 catgtggaag gtgggagagt ccagcttgac acttgttttt gccattgtgt ggggattcac
      841 ctttaccaat aaatatctgt agctgctgtc aagatcatta ttaatagtgc tgtacagaat
      901 atcacctgtt gatttcatta tataagagca atatacgggg ccgttttcta tggatttgct
      961 ctccagggtc ttcaggctta ctatattgag cccgctcata tagtctttgg tatacagcac
     1021 atttgagttg tccgaatcgg tatagattcc gttggcaatg cttgtgaatt ctttggtgct
     1081 gaaatcatag aaagcaatca tatcattgta ttcattgttt ttcatctgca caatgtaatt
     1141 gctgtcgata aacttgctcc tgataaacgg ggtatagctg tttactgcga ttgatatgaa
     1201 ttctcttgaa gcgatgtcaa attccatacg ggtatttatc atcagccttt ttgaatcctt
     1261 tgaccagctt aagatgttat aggcgtcccg tttttccgca gtgaccagct tttgcagctg
     1321 tccggttatg ctgctgtaaa tatatatatt gctgtttccg tcaataaaag ccagggaatt
     1381 tcctgtttca tcaaaatgaa aatcctttaa ggagagaaat ttcccgaggc ttttgctctc
     1441 accattctcg gtgctgcaaa tgtagagatt tatttcctgc accatattcc ctatgacaac
     1501 tctgcggttt gtgatatcag gaacagggct tcccgtaggc tggtatgcaa cgaccatact
     1561 atagtcaggc gaaactgcaa ggggaatcat gcctgcctca atcttgccaa ttgcttcttt
     1621 gtatttttct ataggaaata tccgggattc cacattaagg tgctcgtaaa cacttttatc
     1681 ctgccggatt tcattagggc tctcattttc aatgacaacc agctgcccct gctttacggt
     1741 gcatcccgag agcaatgcta ttataactga caatgtcagg attaatcttt tcataaaaaa
     1801 accccctgct attccaatgt tattattata ctggtgcctt tcccggcggt gcttcggata
     1861 tcaatactgc cgccgtgctt atcaattatt tcctttacta tggacaagcc gaggccccag
     1921 ccctcattat ggctgtttct ggatttttcc accttataat agggttcgaa taagctttca
     1981 aggtttttaa tatctattcc gcagccgtaa tccacaactt caagcaccac tttgtctttc
     2041 ttcctgaggc ttatgtctat gaccggagac ttgctgtact tgattgagtt gtcaatcagg
     2101 tttattatta cctgtttgaa gaaatcagga tccaccttcc atgtgatatc ttccagatct
     2161 agcctgcagc ttgtattgta ccttttcatc ttataatgca tggaatcgca tgcatcctct
     2221 atcagcttct taaggtttga atcggacttg ttcagttcaa agttgtaatt cttcagcttt
     2281 gaaagctcaa ggagcctctc catcatcttc agcattcttt tcccttccga agtaatgtaa
     2341 aacaggcttt tgtccttcac attctcatca ttggttttcc acaacaggtc ggcatatccc
     2401 aggattgtag taagaggagt tcggatttca tgggtaaaat tatcgaagaa atatttttgt
     2461 ttttcttttt catgattaag cttgtttatc atatcttcaa tactgtccgc cattgaattg
     2521 aaggtaaccg aaagctctcc cacctcatct ctggttgctg tacaagcacg gctttcaaag
     2581 ttgccttctg aaaactgcct ggtagcttca ttcagcattt ttatcggctg ggtaatcctg
     2641 tttgaaatta ttgtgcttcc cagcatcatg aatatagaca ggaaaactcc cgtgaagacg
     2701 aacatcctca ctgtattgtt cttcatattg tcaacttcgc tgaggtcgta tatgaatccc
     2761 agggagtagg tatatctgtt gctgatgctc acgggtactg cataatagaa ggttctgttt
     2821 tttcctgccg caacaaaata ggcctttttc attttaaagg tttcctggat ttccggcctt
     2881 atctctccgt tgaagcccgt tgactcctcg gagtccccaa gaagctcagt gccatagtat
     2941 atctgtactc tgcattttac caggtcattg agcacagctg ccagataatt ggcgcttgca
     3001 tccatgaccg aaggtacttc aaagtcgttg ggagctttgc tcagggcata ctgttctata
     3061 tatatggctg agtaattccc ctgattttca agatacttga gagtggtgga aaggttgtag
     3121 ttgtctgtta ttgcagtgat tatcgttccc agtattatga gcatgaaaaa aagagcaccc
     3181 atattaagtg caatgatttt atgcttaagc ttcatacctg tacctcaaat ttataaccga
     3241 tgccaaatac ggtttttata aaaaagctgt attttccgag tttctttctc agccgctgca
     3301 catgcatgtc cacagttctg ctgtcaccga tgtattcaaa tttccacacc agttcaagaa
     3361 ggttctccct ggagaagact tttctctgat tttcgcagag aaccagaaga aggtcatatt
     3421 cctttggagt cagtacgatt tcttcctcat ccacatagac ctgccgctcc gatgtgttaa
     3481 tcattatggg tcctaattgt atattgggat catccttggg ttctgattta ttaattcttt
     3541 tgaggacggt tctaatcctc gcggtcaatt ccctactgtc gaagggcttg gtgatatagt
     3601 cgtcggcacc cagttcaagg ccataaagct tgtccttaat gtcgttcttc gccgtaagca
     3661 taataattgg tatgcttaaa tcctgaagct tcttgcagac ttcaaatccg ttgatatccg
     3721 gaagcattat atccagcaat atcaagtcgg gcataaaaat cggcacagct ttgacagctt
     3781 gcttgccggt atagcataca caagtatcga agccttccag ttcgagattt atctttatta
     3841 ggtcaacaat tgccggttca tcatcaacta tcagaacttt caaagagatc acctgcctgt
     3901 aaagattcgt gcaaataaac tgtgtgcact aatattttaa cataaaaaga cgttggtgtt
     3961 gtatatattt tgtaacatgt attaattcag aacggtaaca ttcttctcat ttcaatgaaa
     4021 actctttttt aatccgctcc agcagttctt tatccgtcaa gatatcatag cccgtaagcg
     4081 caagggcaca ggcgccttta accagtgcat catgagccct gtccgtcagg gttgcatctc
     4141 tgaattcagt agtatgacct atgagatcgg tatccgatat ggagatgtaa ggatgtattg
     4201 ccggaaccac atggcttaca ttacccatgt ccaaagagcc gaaactaaac ctttcggcgt
     4261 tgatttcacc gacacccgca agcctgaggt tctctgtaaa ggtttttgac atatttatat
     4321 tcgtattcat atcatcatag gatatctcat agttacttat ttccaaagct gcaccggtca
     4381 tgagagcagc ccctcttgca atattcttta ctttctcagt cacttctttg aggtaatcct
     4441 ttttccccgc tctaacatag aattgcgcga cagctttact tggcaccaca ttggcagctc
     4501 tgcccccttc agcaattatg ccgtgtattc ttacgtcaga tgtaacgtgc tgcctcagag
     4561 cattaatacc gttaaaggtt agtattacag catccagtgc attaatgccc tcatggggag
     4621 aggaggctgc atggcttgat ttgcctctga aatcaaattg aattgcatcc attgccaaag
     4681 agcttccgct ttcatatgtc ttgtcctcag gatgcaatat cattgcagca tcaataccat
     4741 cgaatactcc tttttctgcc atatccacct ttgctccgtc tgtttcttcc gccggagtgc
     4801 ca
//
LOCUS       DAOQUR010000102         4644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_20617_length_4644_cov_12.009370, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000102 DAOQUR010000000
VERSION     DAOQUR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20617_length_4644_cov_12.009370"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..2662
                     /locus_tag="PKI50_12025"
     CDS             257..2662
                     /locus_tag="PKI50_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04467.1"
                     /translation="MKKVLRAAAFILMLALIIGRPAMQLLQAEAKDEIRIDMKVGFDK
                     FYKIGFAAPVYFEIENSLRDINGELQVEMPNQYGSITIYAINVNLPRNSVKKFLMNVP
                     MNVFNTKINVNLAEGDNIISKKSFRIDPGLSADTYAIGMLSDDFDSVKYINKVSMKSF
                     GNISTKNVKLDEDSFPEDMDVLKAFNVIVINNYDTSRLSTAQYETLKRWVSEGGMLII
                     GTGPSHNKTLAVFKDDFITGEVGEVSTLTTSSLNEMAGSKTGPAMSISVSDITMNNST
                     PGIKDGDFVLLQKIEKGKGVVGVASFDFGMEPLSTWVGNSAFADNLIVGIMPQYYFSD
                     IYQKRIIVRDDFYAIDNALRNIPELPQPKPFHMVFIYIAYILLAAPISYFILKRMDRR
                     ELMWLTVPVLSIAFSGVVYLTGAGTRLTGPVTNVISLVDIDNSGIISPKVYAGVFTPN
                     KDNIRIEAAGDFKVKPLRMNDNYYRGPIDNENTSGRIDSKITLAPRTVLEFYKSGVWS
                     MKTLAMETGKEFSGKLESNLNYAKGSFTGTIINSSGFDLDECYIITPTQYAGVGPVKN
                     GETKQIDVKPSSYFGQRYELINAIYKDPYSGSNRVNRNTKLTPEEMTEFRLNLQKRHV
                     LEHSLMYEAYQGYEAQLIAWSSTPVAEDLLVNGRETRKYEKAFITSKVNLRFRDGKNV
                     EYPLGFLKPEIVNNLDAGNYDDYGKTFYGRGSFEVHYKIDDIRAESINIQYTVGDTQR
                     VKQYIWDNEEKEWDEGDYRDFEIHGELLGKYLDGSSMLKLKIELSDSDAQLPRISVKG
                     SVK"
     gene            2662..3591
                     /locus_tag="PKI50_12030"
     CDS             2662..3591
                     /locus_tag="PKI50_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04468.1"
                     /translation="MLSIRNLNKRYGRFQAVSDLNLDVPEGEIFGFVGPNGAGKTTTM
                     KIICGLLRATSGEITLDGVDIIQNSRRMKEKIGYMPDFFGVYDDLKVSEYLEFYASIY
                     NIKGQERKRITDDLLELVDLGSKREAYVDSLSRGMKQRLCLARSLVHNPRLLVLDEPA
                     SGMDPRARFEMKEILKNLKGMEKTIIISSHILPELAELCTSIGIIDQGRMVISGSNEE
                     IMQQVYNKKVVRLKVRDRLDDAVLILKEYPFVDKLVIGENTIQAGFGGGDGEMSRVLS
                     NLINRGIPVVSFAQMDGNLEDVFMKVTKGDDEQ"
     gene            3588..4499
                     /locus_tag="PKI50_12035"
     CDS             3588..4499
                     /locus_tag="PKI50_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04469.1"
                     /translation="MNINPVLLKELKVRMRGWKAAGIIALYLLVLTVVAVFIIYTTFM
                     DPYSSNIDPQVSIGAYTALAVFQFMLIMFIVPALTAGAISGEREKQTLDLVLCTRLKP
                     ISIITGKLFASTSQTLLLIIASFPLFSMVFLFGGISIKEVMQLFGFYIVTAVTIGCIG
                     IFFSAHLKRTTASTVFTYGTLAFLAFGTIFIGVFYIRIFYNWDYNKFLPILYSNPLTG
                     FGSLLAEQFGYYGGINAFLGLSAGSGQNPNAANAISPWLGNIIFDIVLSAVLLILSAA
                     RINPVRKSIFSYIRFRGRKKKKASDSI"
BASE COUNT         1403 a          852 c         1187 g         1202 t
ORIGIN      
        1 aattaagtca gacaagactt aattatattt aaattcgcat atgaagaata atgcgacggg
       61 gacacgggga cgtttcgcgt gtcccactga aagcgggaca ggggaaacgt ccccatgtcc
      121 ccgggagaaa cgtccccgtg tcctcgtgtc gcattcttca tatactgcga aacatttgcc
      181 ctatgcattg cgtctataat aatgggtatg actgttcata atgaaagaaa aattgacttg
      241 atacgggggt taagcattga aaaaagtatt aagagcagct gcattcatcc taatgcttgc
      301 tttgattatt gggcggcctg cgatgcagct cttgcaggct gaggcaaagg atgaaataag
      361 aatcgatatg aaggtgggct ttgacaaatt ctataaaatt ggctttgctg caccggtata
      421 ttttgaaata gaaaacagcc ttcgggatat aaacggtgag ctgcaggttg aaatgcccaa
      481 tcaatacggc agtattacaa tatatgcgat aaacgtaaat ctgcccagga attccgtcaa
      541 aaagttcctg atgaatgttc ctatgaatgt attcaacacc aagattaatg taaatcttgc
      601 agaaggggat aacattataa gtaaaaaatc cttcaggata gatccgggat taagtgcgga
      661 tacctatgcc atagggatgc taagtgacga ttttgacagt gttaaatata taaataaggt
      721 gtccatgaag agcttcggca atataagcac aaaaaacgta aagctggatg aggatagttt
      781 tccggaggac atggatgtgt tgaaggcatt caatgtaata gtcataaaca attatgacac
      841 ttcaaggctc agtacagcac agtatgagac tttgaagagg tgggtgtcgg agggcggaat
      901 gcttattata ggaaccgggc cttcccataa taagaccctg gcagtcttta aggatgattt
      961 cattacggga gaggtaggag aagtgagtac actcacaacc tcaagcctga atgagatggc
     1021 agggagcaag accgggccgg caatgagcat aagcgtatcg gatataacca tgaataacag
     1081 cacaccaggg attaaagacg gtgattttgt attgctgcag aagatcgaga aaggcaaagg
     1141 cgtggtggga gtagcctcct ttgacttcgg aatggagccc ctttccacat gggtcgggaa
     1201 cagcgccttt gcggataact tgattgtggg cataatgccg cagtactatt tcagtgacat
     1261 atatcaaaaa agaattatag tcagagatga tttttatgcc atagataatg cgctcaggaa
     1321 tataccggag ctgcctcagc caaaaccctt ccatatggtg ttcatatata ttgcatatat
     1381 attgctggct gcccccataa gctattttat actgaagcgc atggatagac gggagctgat
     1441 gtggttgacc gtacctgtgc tttcgatagc cttctcggga gtcgtatatc ttaccggtgc
     1501 gggaaccaga ctgaccggac ccgtcacaaa tgtcatatca ctggtagata ttgataacag
     1561 cggaattata agccccaagg tttatgcagg ggtcttcact cccaataaag ataatataag
     1621 gattgaagca gccggagatt ttaaggttaa gcctctcaga atgaacgata attactacag
     1681 agggcctata gacaatgaaa atacttcagg aagaatagat tcaaagatta ccctggctcc
     1741 caggacggtg ctggaatttt acaaaagcgg cgtatggagc atgaagaccc ttgctatgga
     1801 gaccggtaaa gagttttccg gcaagctgga gagcaatctg aattatgcaa agggttcttt
     1861 tacaggaaca ataatcaaca gttcaggctt tgacctggac gaatgctata taattactcc
     1921 gactcaatat gccggtgtag ggcctgtaaa aaatggggaa acaaagcaga tagatgtaaa
     1981 acccagcagt tatttcggac aaagatatga actgataaat gcaatataca aggatcccta
     2041 ttccggatct aatagggtca atcgaaatac caagctgact ccggaagaga tgacggaatt
     2101 caggctgaat cttcaaaaaa ggcatgtgct ggagcatagc cttatgtatg aggcgtatca
     2161 gggctatgag gcacagctca ttgcctggag cagcacccct gtagccgaag atctgctggt
     2221 aaacggcagg gagaccagaa agtatgaaaa ggccttcatt acctccaagg tgaatctgag
     2281 attcagggac ggaaaaaacg tggaataccc ccttggcttt ctgaagcctg agattgtcaa
     2341 taatttggat gcagggaatt atgatgatta cggaaagacg ttctatggac ggggttcctt
     2401 tgaagtacat tacaaaattg acgacatcag agctgagagt attaatatac agtacacggt
     2461 aggagatacc cagcgtgtta agcagtatat atgggataat gaagagaagg aatgggatga
     2521 gggagactac agggactttg agatacacgg agaactcctt ggaaaatatt tggacggcag
     2581 cagcatgctt aagctgaaaa tagaattgag tgattccgat gctcagcttc ctcggatttc
     2641 tgtgaaggga agtgtcaaat aatgctttct ataaggaatt tgaacaagag gtacggaagg
     2701 ttccaggcag tctcggatct gaatctggat gttccggaag gagaaatatt cggtttcgta
     2761 ggccccaacg gagcgggtaa aaccactaca atgaaaatta tctgcgggct tttaagagct
     2821 acctccgggg aaataaccct ggatggagtg gatatcatac aaaacagcag gaggatgaag
     2881 gaaaaaatcg gatacatgcc cgatttcttc ggagtgtatg acgacctgaa ggtaagcgaa
     2941 tatctggagt tttatgcctc aatatataat attaaaggtc aggagaggaa gagaatcacc
     3001 gatgaccttt tggagcttgt agacctcggc agtaagagag aagcatatgt agacagcctt
     3061 tcaaggggta tgaagcagcg gctctgtctt gcccgcagcc tggttcacaa ccccaggctc
     3121 cttgtgcttg acgaaccggc ttccggaatg gatcccaggg caaggttcga aatgaaggaa
     3181 atattgaaga acctgaaagg catggaaaag acgataatca taagctccca catacttccc
     3241 gagctggcgg agctttgcac cagcataggg atcatagacc agggcagaat ggtgataagc
     3301 ggcagtaacg aagagataat gcagcaggtt tataataaga aggttgtgag gcttaaggtc
     3361 agagacaggc ttgatgatgc ggtgttgatt ctgaaggagt atccttttgt cgataagctt
     3421 gtgatcggtg agaataccat acaggcgggc ttcggaggcg gcgacgggga gatgagcagg
     3481 gtgctgtcta acctgataaa caggggaata cctgttgtat cctttgcaca gatggacggc
     3541 aatctggagg atgtattcat gaaggtgaca aagggggatg atgagcaatg aacataaatc
     3601 ctgtgctttt aaaggaactg aaagtaagaa tgaggggctg gaaggcagcg ggaataattg
     3661 ctttatacct cctggtgctt actgttgtag cagtttttat catttacacc acattcatgg
     3721 atccttatag ctcaaacatt gatcctcagg tatccatagg tgcatatacg gctcttgccg
     3781 tgtttcaatt catgcttata atgtttattg ttccggcact tacagcagga gccatatcgg
     3841 gagaaaggga gaagcagact ctggatctgg tgctttgtac aagacttaaa cctatatcca
     3901 ttataacggg gaagcttttt gcttccacca gccagaccct gctgctcatt attgcttcat
     3961 ttccgctttt ctccatggta tttctgttcg gagggatatc aataaaggag gtaatgcagc
     4021 ttttcggctt ttatattgtg acggcggtga ctataggctg tatcggaata tttttctctg
     4081 cgcacctgaa aaggacgaca gcctccacgg tttttacata tggcaccttg gcgttcctgg
     4141 cctttggaac catattcata ggggtattct atataaggat tttttacaat tgggattata
     4201 ataaattctt gccgatatta tattcaaacc cgttgacagg cttcggttcg cttcttgccg
     4261 agcaatttgg gtattatgga ggtattaatg cttttttagg gctttctgca ggatcaggcc
     4321 agaatcccaa tgctgcgaat gcaatcagtc cctggcttgg gaatataata tttgatattg
     4381 tattgtctgc agtgctgctg atactgtcag ctgcaaggat aaatccggta agaaagagca
     4441 tattcagtta tataagattc cgaggaagaa aaaagaaaaa ggcttcggac agcatatagg
     4501 ggttatgggg ttatgaggag tgatatttat gagtcctgtc gataaagaac tgataagaat
     4561 ggtgaggtgc ataagagaca ggctgcatgc cgcttttatc ataaacagta ttatacttgg
     4621 gtttatattg gcattgtgtc ttgg
//
LOCUS       DAOQUR010000103         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_21068_length_4568_cov_12.649679, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000103 DAOQUR010000000
VERSION     DAOQUR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21068_length_4568_cov_12.649679"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..558
                     /locus_tag="PKI50_12040"
     CDS             16..558
                     /locus_tag="PKI50_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT04470.1"
                     /translation="MSIHYEEVSEDNIICIKDLCNDLMTYQKSKAYIHPEWFDNMSYE
                     TRVIPSVKSAKANYIVVAKDEDKIIGYAYSNISSKYTYSGGFATLKPVDFFDFDSVKT
                     DDVGCLSQFFIKEDYRNTGIGSALFEKSMNWLSTFKAISDLFIFVSNGNDNALNFYVH
                     KGFKVSHQILDGFITVLRNN"
     gene            650..1108
                     /locus_tag="PKI50_12045"
     CDS             650..1108
                     /locus_tag="PKI50_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNT04471.1"
                     /translation="MIIRKAILPHVEEMYELVNYYAEKGLMLPRSRSALCENIRDFVI
                     IEIDGQVAGIGALHVFWNDLAELRTLAVRSGMERQGIGRRIVEFILKEAKELGLQKVF
                     TLTYQPGFFEKMGFKVVDKETMPQKVWTDCINCPKFPNCNEICLEINIQQ"
     gene            complement(1139..2188)
                     /locus_tag="PKI50_12050"
     CDS             complement(1139..2188)
                     /locus_tag="PKI50_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNT04472.1"
                     /translation="MRKKCSKTGFIFVLIIMMVALTANITYADSDPSQPSNWAKAEIE
                     KAIEINLLPERLQGEYGSNITREEFSEVAVNLYEAISGKEAELQGENPFTDTQSTEVR
                     IANNLGIVKGIGDGRFAPDNAITRQEISVMMYRTLQAAKPGYDYTKKYEHIFADYDMI
                     STWAREAVSYLYVMEVVNGVGDNRFSPKGNTTREQAVVLAKRMHDKVSASKDNLIASR
                     DGTSRQENDLKLKLAKLISQELGKPYKWGGTGPDGYDCSGLVYFIYGKLGIPLPRTAR
                     DQAGAGIYVSKEALQYGDLVLFARDGKNINHVGIYVGDGMMVHSPQTGDVIKKTTIMS
                     GYYERCYYTARRVIE"
     gene            complement(2316..3713)
                     /locus_tag="PKI50_12055"
     CDS             complement(2316..3713)
                     /locus_tag="PKI50_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HNT04473.1"
                     /translation="MKSIDTKKLHSSGFVENARILTASAILIYLAVSLYFMNHFFFNT
                     VINGADVSLKAYNEADDVIIRYIKDYKLQLIERNEKTEEIAGQDIGMKFNRNNSISKI
                     HKMQTSFKWPISLFKEQSFYVNDLYFYNRENLEDRIDNLDCLNKDITEPQNVSFKYVN
                     GCYETVKEIYGNKLHKGRLYEVIKSSILRGQRELDLDKSLCYINPKYTLSSAETSQTQ
                     SLLNKYVSAKITYILRSEKEILDQNTISQWLSVDEGLEVVIDENAVRRYVKGLSKKYD
                     TAGAARNFKTSVNKTLEVKGGFYGWKTDIAAEAQALTENIKRGEVLEKEPIYIQKALS
                     RDENDIGDTYVEINITRQYLWYYKAGRLIAQGHIVSGNPNRGNATSLGVYMLNYKETD
                     TTIRGPGYEAEVTYWMPFNGNIGIHDADWRYSFGGNIYKRNGTHGCVNVPLYLAKRIY
                     ENIEVGTPVICYEEQ"
     gene            complement(3924..4094)
                     /locus_tag="PKI50_12060"
     CDS             complement(3924..4094)
                     /locus_tag="PKI50_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04474.1"
                     /translation="MGIVKDRIINMLGRSIKNAMEPEIIAENTKKAMKRTIPAKYINN
                     YLPNKNYVYGMS"
     gene            4144..4440
                     /locus_tag="PKI50_12065"
     CDS             4144..4440
                     /locus_tag="PKI50_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT04475.1"
                     /translation="MGNKVNLWSKIELNKTEIALSLAIKLLLFSGLLGSIIGMISILA
                     SLSNLSKIGPNLAVSFLTMLYSVLFVFILLPVQSKVKAMILSMDKEYIDEKSAN"
BASE COUNT         1247 a          938 c          802 g         1581 t
ORIGIN      
        1 aggaggaaaa caaatatgag tatccattat gaagaagttt ctgaagataa tattatttgc
       61 atcaaggatt tatgcaacga tttgatgact tatcaaaaat ccaaagcata tatacatcct
      121 gagtggtttg acaacatgtc ttatgagacc agagtgattc cgtctgtcaa aagtgccaaa
      181 gccaactata tagttgtggc aaaggatgaa gataaaatta taggttatgc gtatagcaat
      241 atttcttcga aatatacata ttcaggcgga ttcgcaactc ttaagcctgt agatttcttt
      301 gattttgatt ctgttaaaac cgatgatgtc ggctgccttt ctcaattttt tataaaggaa
      361 gattatcgta ataccggcat aggaagtgct ttatttgaaa agtcgatgaa ctggttaagc
      421 acctttaagg caataagtga tttgtttatc tttgtgtcaa acggaaacga caatgcgctg
      481 aatttctacg tgcataaagg ttttaaagta agccatcaaa tacttgacgg gtttataaca
      541 gtgttaagga ataattaacc atttcaaaat ttttatgcat tagtattgaa atattatgca
      601 tcatttgtta tgatatgcat tatatataat actaaggcag gtgtttgtgt tgattattcg
      661 taaggcaata ttgccccatg ttgaagaaat gtatgaatta gtgaattatt atgcagaaaa
      721 aggcttgatg cttccccgct cccgcagtgc cttatgtgaa aacatccgtg attttgtcat
      781 tattgaaata gacggtcaag tcgcagggat tggagcactt catgtgtttt ggaacgacct
      841 ggcggagctg cggaccctgg cagtcaggag cggtatggaa agacagggaa taggcagaag
      901 gattgttgag tttatattaa aagaagcaaa ggaactggga cttcaaaagg tatttacact
      961 gacttatcag cccggatttt ttgaaaaaat gggctttaag gttgtagata aggaaactat
     1021 gcctcagaag gtctggacag actgcataaa ttgccccaag tttcctaact gcaatgaaat
     1081 ctgcctggaa ataaatattc agcaatagaa aaaaatacgc cttgcggcgc atttttttct
     1141 attcaataac ccttctcgca gtataataac atctctcata atatcccgac ataattgttg
     1201 tcttctttat cacatctccc gtttgaggag agtgcaccat catgccgtct cccacgtata
     1261 ttccgacgtg gtttatattc tttccgtccc ttgcgaaaag caccaaatca ccatactgca
     1321 gtgcttcctt tgatacataa ataccggctc ccgcctgatc ccgcgctgtt cttggcagtg
     1381 gtatgccaag cttgccatat atgaaataca ccaatccgga gcagtcatag ccgtcaggcc
     1441 ctgtacctcc ccacttgtat ggcttgccca actcctggga gataagtttt gccaatttta
     1501 attttaaatc gttctcctgt ctgcttgtcc catctcgtga cgcaattaga ttatccttgg
     1561 atgcagaaac tttgtcatgc attctttttg caagaactac cgcctgttcc ctggttgtat
     1621 ttcccttggg gctgaatctg ttgtctccga ctccgtttac aacctccatt acatataaat
     1681 aacttactgc ctcccgtgcc catgtggaaa tcatatcata gtccgcaaat atatgttcat
     1741 acttttttgt ataatcgtat ccgggttttg ccgcctgcag tgtacgatac atcatcacac
     1801 tgatttcctg ccgggttatc gcattgtcgg gagcaaacct gccgtctcca atgcccttta
     1861 ctattcccag attatttgct atcctgactt ccgtgctttg cgtatcggta aaaggattct
     1921 ctccttgcaa ttcagcctct tttccgctta tggcttcata cagattcaca gcaacctcgc
     1981 tgaactcttc tctggtgata ttgcttccgt attctccctg cagcctttcc gggagaagat
     2041 tgatttctat tgctttttca atttcagctt ttgcccaatt acttggctgg gacggatccg
     2101 aatcagcata tgtaatgttt gcagtcaatg caaccatcat aattattaat acaaaaataa
     2161 agccagtttt actgcatttc tttctcatcc tctacttcat cctctcttat gaagcttgtc
     2221 tttgttatat acatgatatc acaaaactcc attgaatgga gcggctttat tattccattt
     2281 cagttataat ttgtatttcc tgcatacccg cactgtcact gctcttcata acaaataaca
     2341 ggcgttccta cttcaatatt ttcataaatt cttttggcta agtataacgg aacatttacg
     2401 catccatggg ttccgttcct tttatagata tttcctccga aagaatatct ccagtcagca
     2461 tcatgtattc ctatattccc gttaaaaggc atccaatagg ttacttcggc ttcataaccg
     2521 ggccctctta tagtagtatc agtttcctta taattcagca tatagacacc gagactggtg
     2581 gcgtttcccc tgttgggatt gcctgatact atgtggcctt gtgctataag cctgccggct
     2641 ttataatacc acaaatactg ccttgtaata tttatttcca cataagtgtc ccctatatca
     2701 ttttcatccc gggataaagc cttttgtatg tatatgggtt ctttttcaag cacttcgccg
     2761 cgttttatat tttctgtcag tgcctgggct tcggcggcaa tatctgtttt ccatccgtaa
     2821 aaaccgcctt tcacttctaa tgttttattc acggatgtct taaaatttct tgcagcaccg
     2881 gctgtatcat attttttgct taatcccttt acatagcgcc tgacagcatt ttcgtctatt
     2941 accacctcca ggccttcatc aacactgagc cattgactta tggtattttg atccagtatt
     3001 tccttctcgc ttctgagtat ataagtgatt tttgccgata catacttgtt cagcaggctc
     3061 tgagtttgcg aagtctcggc ggagcttaag gtgtacttgg gatttatgta gcataggctt
     3121 ttatccagat ccaattcccg ttgtcctctt aatatgctcg attttattac ttcatataat
     3181 ctgcctttat gaagcttatt cccatatatc tcctttactg tttcatagca gccgtttaca
     3241 tatttgaagc tcacattttg aggctctgtg atatctttgt tcaggcagtc cagattatct
     3301 attctgtctt ccaggttttc tctgttataa aagtataaat cattaacata aaaactttgt
     3361 tccttaaata atgaaatcgg ccatttaaac gaggtttgca ttttatgaat tttagaaata
     3421 ctgttatttc tattgaattt cattccaata tcttgtcctg caatttcttc tgttttttcg
     3481 tttcgctcaa tcagctgcag cttataatct ttaatatatc ttataataac atcgtctgct
     3541 tcattatagg cttttaatga aacgtctgct ccgtttatta cagtattaaa aaagaaatga
     3601 ttcataaagt ataatgaaac agccaaatat atcagtattg ctgaagctgt tagaattctg
     3661 gcattttcaa caaacccgct gctatgcagc ttttttgtat caatactctt catatcggct
     3721 aagatccttc atctgacaca aatatattca gccatgatgg ctatttaatt ctatgtttgt
     3781 attttcaatt ttaaccctca aaaaatcacg gcaggcacat cagtcagaaa gctgtatttg
     3841 atgctttcat cgcgatatta accggaacta cttcttaatt acttccgctg gagtatggct
     3901 ttcaccccgg atatggatta atattaagac ataccataca cataattttt attcgggagg
     3961 taattattta tgtattttgc aggtatcgtt cttttcattg ctttcttggt attttctgca
     4021 ataatttcgg gttccattgc attttttatc gatcttccca gcatattaat tatcctgtct
     4081 tttactattc ccatgctaat ggcatccggc ctttttacgg acttcatcag aggcttcaaa
     4141 ataatgggta ataaagtaaa tctttggtca aaaattgaac ttaataagac agagattgct
     4201 ctaagtcttg caattaagct attgctgttt tcagggctat tgggaagcat aatcggcatg
     4261 atttctatcc tggcaagctt aagcaattta agtaagatag gcccaaacct ggcagtatca
     4321 ttccttacaa tgctttactc agtacttttt gtttttatac ttttacctgt acaatctaag
     4381 gtaaaagcaa tgatattatc gatggataag gaatacatag atgaaaaatc agctaattaa
     4441 aatattgggg ttatccgatt tagccggttg gggtattgtc gagtcacttt tgccaatgct
     4501 ttatggcagc ataatacatt tgatacttaa catgcagaaa tcaaataaaa ctcccttaat
     4561 gggagttt
//
LOCUS       DAOQUR010000104         4560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_21121_length_4560_cov_11.381354, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000104 DAOQUR010000000
VERSION     DAOQUR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21121_length_4560_cov_11.381354"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKI50_12070"
     CDS             <1..253
                     /locus_tag="PKI50_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04476.1"
                     /translation="GNEPYYENISEFEKDIGILKEIGINSFSLFSLDGIIEKSQLLGY
                     LAAMERAKPYKPAICSRVIRNEEISGLLFSIGKLYYRLR"
     gene            406..1473
                     /locus_tag="PKI50_12075"
     CDS             406..1473
                     /locus_tag="PKI50_12075"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT04477.1"
                     /translation="MNLPSSILKPGMTAYEDIFNDLGVLILNKNTILDQWQIEKIHAN
                     GVDRVRVRLTENDSLYAVQEEEIPSIYDPGKIRAFKEKYVAKVDEVTYIIKEIGRGAA
                     VDVQTVGQISRHIIREFSTVSEVINYLHLARPLDDYTYSHSLNVSLMSIIIAKWLNLG
                     NEEIDEIATAGLLHDIGKTKIAERLLQKPGKLTKSEFEEVKKHTIFGYMMMDNILDAT
                     ANMKYSVLMHHEKIDGSGYPIGSLDEDIPLFPKIVAIADIYDAMTSNRSYRNRMCPFE
                     VIKNFEMHTFGKLDTKVLTVFLKNIANSYINDFVELNSGELCEVVFINPNRIWQPIVR
                     SGADYIDLSRDKDKRFIKAII"
     gene            1555..2097
                     /locus_tag="PKI50_12080"
     CDS             1555..2097
                     /locus_tag="PKI50_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04478.1"
                     /translation="MKRYLNYFAAMFLVLAVTDLCLGGALTGFRGQNSDSAPEGLNEV
                     NNIQIQPEVQENAEVMNIDELGEFIASKSCEFVQAAFERDRSKVESMLCKGTMYVVSE
                     DNSSYIRYTSGDLHVEGYMATDRKLMQVRQSWHITEDDGTIASGVEVFIEGEEAPQIW
                     YIHYRRSFGRWKIFMLENGI"
     gene            complement(2104..3756)
                     /locus_tag="PKI50_12085"
     CDS             complement(2104..3756)
                     /locus_tag="PKI50_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT04479.1"
                     /translation="MNIDRMKNLLFDLCSIPSISETPGEIEMAEKIYETVMNMDYFKN
                     NPANAGINPIKNDPFGRYYVHALMEGKPESKKTVILLSHFDVVNVEDYGAYKDYAFKP
                     LEYTELLKNGSGISLSKEAETDLASGDYIFGRGIMDMKFGLALDVEIMHQIGNKLDNF
                     PGNILLLSVPDEENNSAGMLASVEMLAALKNEKGLEYVCCIVSEPHFPKYPGDNSKYI
                     YTGAVGKLLPVFYCVGKETHAGDPFSGLNPNLLTAKIIEEIELNPELSDVSASFRTPA
                     PVCLKQSDTKNSYSVSTPAAAYTYFNFITVTSSPDEVLNKLMGISKKTFEEVLHNIEN
                     KADRLQALTGSKPKLPSIKPMVITFRELYKMCLEVGEKEFDEHMESFVKKSPESDLRE
                     LSVDIVREAHKFCPYRDPMIVLFLAPPYYPHSGARGQNGKIAEITTKIIKKANSKYGV
                     NLYLEPFFPGLSDMSYLGLPDNIDVESLKTNLPLWGDKYTIPLDAIASLNIPFMNIGP
                     LGKDAHKYTERINIPYSFEIASKLVFEAVLSALDASPEDFGI"
     gene            3939..4511
                     /locus_tag="PKI50_12090"
     CDS             3939..4511
                     /locus_tag="PKI50_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04480.1"
                     /translation="MFKKKKNYLKQIYKMNPDTNAYIIEVSLIDYNEIFNGWDPSPIK
                     RRDVDPELISFLEECDSDIPLEFPLELMFYLPEDQYDKEKEKLSRVGIKNYFDYSVHF
                     IRKELLQIRQKIFFYIVMAVAFLSVGYLSGQKAEPGFFTVILIEGLTVGGWVFLWEAF
                     SLFFFARQEVSGRLKRYIRLKNTKISFKYN"
BASE COUNT         1373 a          822 c         1009 g         1356 t
ORIGIN      
        1 gggcaatgaa ccatactatg agaatatctc cgaatttgaa aaggatatcg ggatattaaa
       61 ggaaatagga attaacagtt tttctttgtt cagtttggat ggtattattg aaaaaagcca
      121 actgttgggt tatcttgctg ctatggagag agcgaagccg tacaaaccgg caatatgcag
      181 ccgcgtgata aggaacgagg agatttccgg actgcttttt agtatcggga agctttatta
      241 taggttaaga tgaaagaata atgattatcc tataattgta atatcatgta aatatcaggt
      301 ttattttgac gtaataaagt ggtataataa ttctagatat ttacaagaat ttgcaattgc
      361 tatattgcct atatatcatt taggaggtga gagtctgtga aagtgatgaa tcttccgtca
      421 agcatactaa aacccgggat gacagcatat gaagatattt tcaatgattt aggggttttg
      481 attctcaaca agaatactat actggatcaa tggcagattg agaaaatcca tgcgaacggt
      541 gtagatagag tcagggtcag actcaccgag aatgatagtt tatatgcagt tcaggaagaa
      601 gagataccca gtatctatga tccggggaaa ataagagctt ttaaagaaaa gtatgttgcc
      661 aaagttgacg aggtaactta cattattaaa gaaattggca gaggtgctgc tgtagacgta
      721 caaacagtcg gccagatcag caggcatata ataagggaat tcagcactgt aagtgaagtt
      781 ataaactatc ttcatctggc aaggcctttg gatgattata catattccca ttcactgaac
      841 gtatcgctta tgtcaatcat aattgcgaaa tggctgaatc ttggcaatga agaaattgac
      901 gagattgcga cagcaggatt gcttcatgat atagggaaga ccaagatagc cgaaaggctt
      961 ctgcagaaac ccggaaagct tacgaaaagc gagtttgaag aggtaaagaa gcatacgatt
     1021 tttggatata tgatgatgga taatatcttg gatgctacag ctaatatgaa gtattcagta
     1081 ttgatgcatc acgagaaaat tgatggttca gggtatccca taggctcatt ggacgaagac
     1141 atacccctgt ttcccaagat agtagcaata gccgatatat atgatgcaat gacctccaac
     1201 agatcctacc ggaacaggat gtgccccttt gaggtaatca agaattttga aatgcatacc
     1261 tttggcaagc tggataccaa agtgctgaca gtgtttctga agaatattgc caattcttat
     1321 attaatgatt tcgtggaact caatagcggt gaactgtgtg aggtcgtgtt cataaacccc
     1381 aatagaattt ggcagcctat agtcaggtcc ggggcagatt atattgattt gtccagggat
     1441 aaggataaaa gattcattaa agcaataata tagataatat actctgtatt atattaagca
     1501 gcttggcaaa taataacttc actatgagtt tcaataataa ttggggtgga cacagtgaag
     1561 agatatttga actattttgc tgccatgttt ctcgtccttg cagttaccga tctctgtctt
     1621 ggaggcgcac tgacagggtt cagggggcaa aactccgatt ctgcaccgga aggcttaaac
     1681 gaagtgaata atatccaaat tcagccggag gtacaagaaa atgcagaagt gatgaatatt
     1741 gatgaattag gcgaattcat tgcttcaaaa tcctgtgaat ttgtacaggc ggcttttgag
     1801 agggacaggt caaaagtcga gagtatgctt tgcaagggta cgatgtatgt ggtttctgag
     1861 gataattcca gttatataag gtacacctca ggggatttgc atgtggaagg ctatatggcc
     1921 actgacagga agcttatgca ggtaaggcag agctggcata taacagagga tgacggaacg
     1981 atagcctccg gagttgaggt ttttatagaa ggggaagaag cccctcaaat atggtatata
     2041 cattacagga gaagcttcgg ccgatggaaa atattcatgc tggaaaacgg gatatgatac
     2101 ttgtcatatc ccgaaatctt cagggcttgc atcaagggct gacaatactg cttcaaacac
     2161 aagctttgaa gctatttcaa aggaatatgg gatattgatc ctttctgtgt atttatgtgc
     2221 atcctttccc aaaggcccaa tattcataaa tggtatattg aggctggcta tcgcatccaa
     2281 ggggattgtg tatttgtccc cccacagagg aagatttgtc tttaagcttt caacatctat
     2341 attgtccggc agccccagat agctcatatc ggacaagccc ggaaagaagg gctccaaata
     2401 tagattcaca ccatattttg agtttgcctt ttttatgatt ttcgttgtta tttcagctat
     2461 tttgccgttc tgtccccttg ctcccgagtg ggggtagtaa gggggtgcga ggaacaggac
     2521 tatcatagga tcacgatagg ggcagaattt gtgtgcttcc ctgactatat caacggacaa
     2581 ctccctaaga tcactttccg gtgatttctt tacaaaggac tccatatgct catcaaattc
     2641 tttttcccca acttcaaggc acattttgta aagctcacgg aatgtaataa ccataggctt
     2701 tatggatgga agctttggtt tgctgcctgt aagggcttgg aggcggtcag ccttgttttc
     2761 aatgttgtga agtacttctt caaatgtttt tttgctgatg cccataagct tattaagtac
     2821 ttcatccggt gaagaagtta cggttatgaa attaaaataa gtgtaggctg ctgccggagt
     2881 ggagacagaa taagagttct ttgtgtctga ctgtttaaga cataccggag caggcgtcct
     2941 gaagcttgcc gatacatccg acagttccgg gttaagttca atttcttcta ttatttttgc
     3001 cgtgagaaga ttcggattca agccggaaaa aggatctccc gcatgggtct cttttccgac
     3061 gcagtaaaag accggcagta gttttccgac agcccctgta tatatgtact tgctgttgtc
     3121 tccggggtat ttcggaaaat gcggttctga tacaatgcag catacatatt ccagtccctt
     3181 ctcattcttc aatgcggcca gcatttcaac agaagccagc attcctgcgg aattgttttc
     3241 ttcgtcagga accgacagca acaggatatt tcccgggaaa ttgtccaatt tatttcctat
     3301 ctgatgcatg atttcgacat caagggctaa tccgaacttc atatccatta ttccgcggcc
     3361 aaaaatataa tcacctgagg cgagatccgt ctcagcttct tttgagaggc tgatgccgct
     3421 gccatttttg agaagctcgg tatattccaa aggcttgaat gcataatcct tataagcgcc
     3481 ataatcctct acattcacaa catcaaagtg gctcagcagt ataacggttt ttttgctctc
     3541 cggctttccc tccatcaagg catggacata gtatcttccg aaggggtcat tctttatcgg
     3601 gttaattccg gcattagccg ggttgttctt aaaataatcc atattcatga ctgtttcata
     3661 gattttttct gccatttcaa tttctccagg tgtctcagat atgcttggaa tactgcaaag
     3721 atcaaacagc agatttttca ttctatcaat attcattaat actcctccgc ttccgtgtat
     3781 tctgcactaa acactttgca ttgtattcac atgtgcaaaa tatatactta cattttacca
     3841 gaataaatgg tgcctggcat ccggaaagtt tacaaaagcc agtaattggg ataatatata
     3901 cataacatca gtatttatgc catgtacgga ggttcctcat gtttaagaaa aagaagaact
     3961 atctgaaaca gatatataag atgaatccgg atacaaatgc gtatattatt gaggtctcat
     4021 taatcgatta caatgagatt ttcaacggct gggatccttc tccgatcaag agaagggatg
     4081 tggatccgga acttataagt tttctggaag aatgcgattc cgatattccc cttgaatttc
     4141 ctctggaact gatgttttac ctgcctgaag accaatatga caaggaaaaa gaaaaacttt
     4201 caagagtggg gataaagaat tattttgact atagcgttca tttcatcaga aaagagttat
     4261 tgcaaatcag gcaaaaaata tttttctata ttgtaatggc tgttgctttc ttatcggtgg
     4321 gatacttgtc aggacagaaa gcagagcccg gtttttttac cgttatctta atagaggggc
     4381 ttaccgtcgg cggttgggta ttcttatggg aggcattttc attattcttt tttgccaggc
     4441 aggaggtttc gggcaggctg aaaagatata taagactcaa aaatacgaaa atcagcttta
     4501 aatataatta agtcagacaa gacttaatta tatttaaatt cgcatatgaa gaataatgcg
//
LOCUS       DAOQUR010000105         4451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_21838_length_4451_cov_11.391265, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000105 DAOQUR010000000
VERSION     DAOQUR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21838_length_4451_cov_11.391265"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PKI50_12095"
     CDS             complement(<1..983)
                     /locus_tag="PKI50_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT04481.1"
                     /translation="MGWTEEQARAISEGRCNLLVSAAAGAGKTAVLVERIIRKITQAE
                     NPVDIDRLLVVTFTNAAAAEMRERIGAALSRVLEEAEGDGNPKVKKNLQRQLTLLGKS
                     NITTIHSFCLDVIRNNFHTIDLDPAFRIADDTEALLLQLEALEELIESKYEPENCTED
                     FLSLVECYGGSKDDNKLMDLVLTIYDFVQSHPWPDIWLRDAIEAFNAAEDTDFGDTKW
                     AEVLMKSISMELAGLQCLLEDTIKAAEREEGLSPYINTLQEDILRIKAMQSACKGKGG
                     WDALYREFDNLGFSRLPKCGKEADPIIKEQAKGARDDVKDRLNRIKKDLICG"
     gene            complement(970..4371)
                     /gene="addB"
                     /locus_tag="PKI50_12100"
     CDS             complement(970..4371)
                     /gene="addB"
                     /locus_tag="PKI50_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HNT04482.1"
                     /translation="MSLRIIYGRAGSGKSIYCLNEIKESLNQGNNTRHIIIVPEQFSL
                     QAEKKLIKAVGASGINGAEVLSFRRLSHRVLGEVGGLVRKHINPAGKSILVYTIMEKL
                     RDELRVFAKAAGKQGFVNTLCGTISELKRYNIAPPALGSIETASLSPQLADKLHDIGL
                     IYGEFEKRLHDKYIDSDDDLDILAEKLELYKAFETSEIWLDEFSGFTPQEYGVIAKLM
                     SKAVRVNVLLCTDCLSEDYAPEDTDPFLPARAAAAKLIRLAKDSGIKVDAPVSLKNNG
                     FKFNKELAFLESNFYSYLHGKYKESTEHLKLYEAPGIFAEAESAARDILKKCREEGFR
                     YRDIAVITGDLPQYEKVIRVVFREHGIPCFIDRKKDITGHPLILLVLSALEIFIGNWS
                     YEAVFRYLKTGLTGIDRRESDILENYVLANYVPGSSWMKDEDWEGLSVNEARRRAAAP
                     LLRLYGKIKGKNTVKEICTALFDFLCELEVPEKLEMLVEHFTQRGELDRADEYSQVWN
                     ILLELLDQVVEAIGDEAVKADTFLTILSVGFGEYNIGLIPPALEQVLVGSLERSKSHD
                     VKAIYVLGANDGIFPVPFKQEGILSDRDREILRNMGMELAPDTRGRTFEQQHLIYTAL
                     TASSDYLRLSWCAADREGRAQRPSVIISRLRRMFPEISFESGILRGISGYDADTNVSA
                     PVPTFNELVSAMRQGFEGRYADPLWKSVADWYKKEEQWKEKYFRIAEGLSYTNQAGFI
                     RQERIRKIYGKTIHTSVSRLERYFSCPFSYYVEYGLKAKERPVMKMNAPDMGSFMHRV
                     IDMFSRKVSESDLGWRHLEREWCAGEVSLIVDELVQDAAGKVFLSTNRYKYLAARLKR
                     VLTRAVWLIAEHIKRSAFEPVGYEIGFGEGEEFPAIELELPSGEKMSLSGRIDRLDCM
                     ETEEGKYFRIVDYKSGSRHFKLEDVYYGLQLQLITYLDAVGGSLEEEPDKPVLPAGVL
                     YFRLDDPIIQGSREASEEEIEKAIMKELRMKGLLLADVKVIKEMDTGIDGDSLIIPAR
                     INKGDVLGRSSAATAEQFEIIKRHVRKLLLRLGEEILKGNISISPYRRKKMTACAYCS
                     YMPVCQFDMSISGNNYRNLWEKKDEEIWELMAGKTVEGGEANGLD"
BASE COUNT         1028 a         1277 c          815 g         1331 t
ORIGIN      
        1 ccgcatataa ggtctttttt tatcctgtta agccggtcct tgacatcatc cctggcacct
       61 ttggcctgct ctttgataat agggtctgct tctttgccgc atttcggcaa gcgcgaaaac
      121 ccgaggttat caaactccct gtaaagagca tcccagcccc cttttccctt acaagccgac
      181 tgcattgctt ttatccgcaa aatgtcctcc tgaagggtat ttatatatgg agacagcccc
      241 tcctccctct cggctgcctt tattgtatcc tccaacaggc attgaaggcc ggccagctcc
      301 atcgagatac ttttcatcag gacttccgcc catttggtat ccccgaagtc cgtatcctca
      361 gccgcattaa aagcttctat cgcatccctc agccatatat caggccacgg atggctctgc
      421 acaaaatcat atatggtcag tacaagatcc atcagtttgt tgtcgtcttt acttcctccg
      481 tagcattcca ccaggctcaa aaaatcctcg gtgcagtttt caggctcata ttttgattca
      541 ataagctcct ccaaagcttc cagttggagc agcaatgctt cggtatcatc cgctatcctg
      601 aaggccgggt ccaagtctat tgtatgaaaa ttatttctaa ttacatcaag acagaaggaa
      661 tgaatagtgg taatgttgga tttccccaaa agagtcagct gcctttgaag attcttctta
      721 accttcgggt ttccgtcccc ctccgcttcc tccaacaccc tgctcaaagc ggctccgatc
      781 ctctctctca tttcagcggc ggcggcattg gtaaaagtga caaccagcaa tctgtcaatg
      841 tccacaggat tttccgcttg agtaatcttc ctgattattc tttcaaccag cacagctgtc
      901 tttccggctc cggctgctgc agacaccagc aaattgcagc ggccctccga aatcgccctg
      961 gcctgttctt cagtccagcc catttgcctc accgccttcc acagtcttgc ctgccattag
     1021 ctcccaaatc tcttcgtcct tcttttccca aaggttcctg tagttgttcc cggaaatact
     1081 catatcaaac tggcatacgg gcatataact gcagtacgcg caggcagtca ttttctttct
     1141 tctatagggg cttatggaaa tattcccttt gagtatttcc tctccaagtc tcagaagcag
     1201 cttccttaca tgcctcttta ttatctcaaa ctgttccgcc gtagctgcgg aagatcttcc
     1261 caaaacatcc cccttgttaa tccttgcagg tattatcaat gaatctccgt ctatacccgt
     1321 gtccatttct tttattactt ttacatccgc aagaagcagt cccttcattc taagctcctt
     1381 cattatcgcc ttctctattt cctcttccga tgcctccctg ctgccctgaa taatagggtc
     1441 atccagcctg aaatatagta cccctgccgg aagcacgggt ttgtccggtt cttcctcaag
     1501 gcttcccccc actgcatcca ggtaggttat caactgaagc tgaagtccgt aatatacatc
     1561 ttccagcttg aaatgcctgc ttcccgactt atagtccact atcctgaaat actttccttc
     1621 ttctgtttcc atgcagtcta acctgtcaat cctgcccgac aggctcattt tttcaccgga
     1681 gggcagttcc aattctatgg ccggaaattc ctctccttct ccaaaaccga tttcataacc
     1741 cacaggttca aaagcacttc tctttatatg ctccgctatc agccaaaccg cccttgtcag
     1801 cactcttttc agcctggctg ccaagtattt atacctgttg gtgcttaaaa aaacttttcc
     1861 ggctgcatcc tgtacaagct catccacaat aagcgaaacc tccccggcac accattccct
     1921 ttcaagatga cgccagccca gatcgctttc acttaccttc cgtgagaaca tatcaataac
     1981 cctgtgcata aaagagccca tatccggagc attcattttc attacaggcc tttcctttgc
     2041 ctttagcccg tattccacat aatatgaaaa ggggcaggaa aaatatcttt caagccttga
     2101 tacgcttgta tgtatagttt tgccgtatat cttcctgata cgctcctgcc tgataaatcc
     2161 ggcttgattg gtatagctca ggccttccgc aatccggaaa tatttttcct tccattgttc
     2221 ctccttctta taccagtcag ctacgctctt ccacagtgga tccgcatacc tgccttcaaa
     2281 tccctgtctc attgcggaca ccagctcatt gaaggtgggc accggtgcgg acacattagt
     2341 atcagcatca taccccgaaa taccccttag gattccgctt tcgaagctga tttccggaaa
     2401 catcctccta agcctgctta ttatcacaga aggcctttgc gccctgcctt ccctgtcggc
     2461 cgcacaccag ctcagtctca gataatccga agaggctgtc aatgcggtat atataaggtg
     2521 ctgctgctca aaagtccttc cccgggtatc gggggccaat tccattccca tgttcctcaa
     2581 tatctctctg tccctgtcgg ataaaatccc ttcctgctta aaggggacgg gaaatattcc
     2641 gtcattggcg ccaagtacat atatggcttt tacatcatgg cttttggatc tttcaaggct
     2701 tcccacaagc acctgttcaa gggccggagg tatcaagccg atattgtatt ctccgaagcc
     2761 cacagagaga atcgtaagga aggtatccgc cttcaccgct tcatccccta ttgcttcgac
     2821 tacctggtcc aaaagctcca acagaatgtt ccacacctgg ctgtactcat cagcccgatc
     2881 caattctcct ctttgggtaa aatgctctac cagcatttca agtttttcag gcacttccag
     2941 ttcacacagg aaatcaaaaa gagccgtaca tatttcttta accgtatttt tgcctttaat
     3001 tttcccgtat aatcttaaca ggggcgctgc cgcccgcctt cttgcttcat tgacactcag
     3061 gccttcccaa tcctcatcct tcatccaact gctccccggg acatagtttg ccagcacgta
     3121 gttctccaat atgtccgatt ctctcctgtc aatacctgta aggcctgttt tcaggtatcg
     3181 gaaaactgct tcataggacc aattccctat aaatatttca agggctgaaa gtacaagaag
     3241 tatcaatgga tgccccgtaa tatccttttt cctgtctata aagcaaggta ttccatgctc
     3301 tctgaatacc actctgatta ctttttcata ttggggcagg tctccggtta ttactgcaat
     3361 atccctgtac cggaaccctt cttccctgca ctttttcaaa atgtccctgg ccgcactctc
     3421 cgcttccgca aatattcccg gagcttcata cagtttcagg tgctctgtgc tttccttata
     3481 ttttccgtga aggtatgaat aaaagttgga ttcaagaaat gccagctcct tattgaattt
     3541 aaaaccatta tttttaaggg atacaggggc atccaccttt atgccggagt ctttcgccag
     3601 ccttatgagc tttgctgcgg cagcccttgc cggaaggaag gggtctgtat cctccggtgc
     3661 ataatcctcc gaaaggcagt cggtgcacag caggacgttt accctgacag cctttgacat
     3721 aagttttgca attactccgt attcctgagg ggtgaatccg gagaactcat ccagccatat
     3781 ttccgaagtc tcaaaagcct tgtatagttc aagcttttcc gccaatatat caaggtcgtc
     3841 gtcagaatcg atgtatttat catgaagcct tttctcaaac tccccgtata taagccctat
     3901 atcgtgaagc ttatccgcaa gctgcgggct gagggaagct gtttctatac tgccaagtgc
     3961 tgggggcgct atgttatatc tttttaattc gcttattgta ccgcacaaag tatttacaaa
     4021 gccctgcttc ccggcagcct tggcaaaaac ccgcaattca tcccgcaatt tttccatgat
     4081 cgtatatacc agtatgcttt tccctgcggg gtttatatgc ttcctaacca agccccccac
     4141 ttctcccagc accctgtggg aaagcctcct gaagcttaac acttccgctc cgttgattcc
     4201 gcttgccccc accgccttta tgagcttttt ctccgcctgg agggagaact gctcgggaac
     4261 aattatgata tgccttgtat tgttcccctg attcaggctt tcttttattt cgttgagaca
     4321 atatatactt ttaccgcttc ctgcccgtcc gtatatgatt ctcaggctca ttaggaatcc
     4381 ccgccttttc tggtaacttt tccggtaata tgtatatatt atacatcata taggcctaag
     4441 atacgagact c
//
LOCUS       DAOQUR010000106         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_23304_length_4246_cov_12.438797, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000106 DAOQUR010000000
VERSION     DAOQUR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23304_length_4246_cov_12.438797"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..788)
                     /locus_tag="PKI50_12105"
     CDS             complement(69..788)
                     /locus_tag="PKI50_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT04483.1"
                     /translation="MDVIVLAGEKKDTVNKAFYKIKDKYMIEYVVETLRKSESIDRIA
                     VVGPKDKLEPAIGGIVDIVVEGTDSIVSNILLALEYFPEANKILVVTSDIPMITREAM
                     EDFIARCNEKDVDLCYSIVDKKVNDQKYPGVRRTYARLWEGRFTGGNVFCFNPAVKDK
                     CKDFVEKMLEYRKSPAKMAGVLGFAFLLRLALGILTINAIERKCESLLGIRAAAIISP
                     YPEICNDVDRLSDLEYIEKYM"
     gene            complement(804..1499)
                     /locus_tag="PKI50_12110"
     CDS             complement(804..1499)
                     /locus_tag="PKI50_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT04484.1"
                     /translation="MDNNFLELKLDNYKPLREVVFNSIRGAIISGVLKPGERLMEVQL
                     AEKMGVSRTPIREAIRKLELEGLVVMIARKGAYVADLSIKNITDVLEVRAVLEGLASG
                     LAALRMTEEEIKDLELTARHFEQAMNSNDVEGVIQTDIEFHEKIFKATRNEKLLQLTN
                     SLMEQVQRFRVMYLNKAIKSTNLIKEHYKIVEAISRRNREMAENIAKIHIQNAEKDMM
                     RLLGSSSGKNDNP"
     gene            complement(1529..2398)
                     /gene="ispE"
                     /locus_tag="PKI50_12115"
     CDS             complement(1529..2398)
                     /gene="ispE"
                     /locus_tag="PKI50_12115"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNT04485.1"
                     /translation="MNTLAGRAAAKINLAIDVLRKRPDGYHDVLMIMQSVALFDTITV
                     RALKGDIKVTSNNDRLPADKGNIVYRAAEYLKIKYNVKEGALINIEKTIPIAAGLAGG
                     SADAALTLKLLNKAWNLRLSRNEILDAGKKLGSDVPFCIQGGTALAEGLGEKLTQLPG
                     IPECLILLAKPAVSISTREVYEGLKLEEIRERPDIKSMIRYIDGRNLEGIASCMCNVL
                     ETVTIKKCPQIEELKNKLMEYGALGSIMSGSGPTVFGVFKDSASAYNAYDYIKDMVSE
                     VFLVKTTNQNLDF"
     gene            complement(2551..4140)
                     /locus_tag="PKI50_12120"
     CDS             complement(2551..4140)
                     /locus_tag="PKI50_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HNT04486.1"
                     /translation="MPLELIKNPLKVSRIVGENVFSTVVEEDINVPDINPDLYKILAP
                     GATVQIRDCEVLNDKVVVNGQILLSILYAADDEGKPLNSMDVAANFSQGIEVPGARPR
                     MRESINTVIQHVDCYMINSRKLGVKVIMDMHCKVEDLFDLELASDVRGLSDIQVLREE
                     SSFKQVMGYNKDHYEFNEELNLAADAPAIGKILRSDSKVIIKDEKPIEGKVEVNGLLG
                     IDILYKSETEENQLQYQEFEVPFTQYIEIPAAEKNMECVTESTLQECRLEVNEDANGE
                     RKVINAYVMLGMGARVFNSIEQEVVADAYSPTNVIDIERNIFSLSEFVGKGRSNIVIK
                     ETVGIKHGDPEIEKVCCVNVLPIVNEVKLLDDRVLLEGMAECTFVYESSYSAEPMCSM
                     TEQIPFRHFMDIPGVRLGMPCTVKCSADNVNFSKINNEMIELRVVLGVYAEVMRHIEK
                     KLVENVEAVEGISIDYGRIPAVTIYMVQKGDTLWSIAKRYNTTVDALAKLNNIENPSK
                     VANGTKIMILKNIRMGKDNSL"
BASE COUNT         1043 a         1109 c          687 g         1407 t
ORIGIN      
        1 gcagcaggga cggttctttt tgcttttgaa agcaaaaaga accgtccctg ctgcttcccc
       61 gctgcttctt acatatattt ttctatatac tccaaatcac tgagtctatc cacatcattg
      121 catatttcgg gataagggga aataatcgct gctgctctta tgcccaaaag agactcgcat
      181 ttcctttcaa ttgcattgat tgtcagtatc cccagtgcaa gcctcagcaa gaatgcaaat
      241 ccaagtactc cggccatttt tgcggggctt tttctgtact ccagcatctt ctccacgaaa
      301 tccttgcact tatcctttac agccgggttg aaacagaaca cattccctcc ggtgaaccgg
      361 ccttcccaaa gccgcgcata agttctcctg actcccggat acttctgatc attgaccttc
      421 ttgtcaacaa tggaatagca taagtcaaca tccttctcat tgcatcttgc aataaagtcc
      481 tccattgcct ccctggtaat catcgggata tctgaggtca caacaagaat cttatttgcc
      541 tcgggaaaat actccaatgc cagaagtata tttgatacta tggaatccgt tccttctacg
      601 acaatatcaa caataccgcc gattgccggc tccagcttat ccttcggccc gactacggca
      661 atcctgtcga tgctttcgga ctttctgagg gtttccacca catattctat catgtacttg
      721 tccttaattt tataaaatgc cttgtttacg gtatcctttt tttctcccgc aagcacaata
      781 acatccataa agttcaatcc tccctacgga ttgtcatttt tcccggatga actccccagc
      841 aatctcatca tatccttctc ggcattctgg atgtgtatct ttgcaatatt ttcagccatt
      901 tccctgtttc tcctggaaat tgcttcaact atcttgtaat gctctttgat aagattggtt
      961 gatttgatgg ctttattcag gtacattacc ctgaaccttt gaacctgctc catcagacta
     1021 ttggtcaact gcagcagctt ttcgttcctt gtagccttga atatcttttc atgaaattca
     1081 atatctgttt gtattacgcc ttcaacatca tttgaattca tggcctgctc gaaatgcctg
     1141 gcagttaatt ccagatcctt gatttcctcc tcggtcatcc ttaatgctgc aagtcccgaa
     1201 gcaaggcctt ccaaaactgc cctgacctca agcacatcgg taatgttctt aattgaaagg
     1261 tctgcaacgt atgcaccttt ccgggcaatc attactacca agccttccaa ttcaagtttc
     1321 cttatggctt cccttatagg cgttctgcta acccccatct tttctgcaag ctgaacttcc
     1381 ataagcctct ccccgggttt cagcacccct gagataatag ctcccctgat tgaattgaat
     1441 acaacctccc ttaagggctt gtagttgtcc agttttaatt cgagaaagtt gttatccatt
     1501 attatcaccc cctgttagca ttttaatact aaaagtctaa attctggttg gtagtcttga
     1561 ctaaaaaaac ctcactcacc atgtctttaa tataatcata tgcattatat gcagatgcag
     1621 aatccttgaa tactccaaac accgtcggcc cgctgccgct cataatgctt cccagggcac
     1681 cgtattccat taatttattc ttcaattctt ctatctgtgg gcattttttt attgtcactg
     1741 tttccagtac attgcacata caggaagcta taccctcaag gttccttccg tcaatatacc
     1801 gaatcatact ttttatgtcc ggcctttctc tgatttcttc aagcttcagg ccttcatata
     1861 cctctcttgt agatatgctt acagcaggct ttgccaatag tataaggcat tcaggaattc
     1921 ccggaagctg tgtcagcttc tcacccaaac cttccgccag ggcagttccc ccctgaatgc
     1981 aaaagggcac gtccgaaccc agctttttcc ccgcatccag tatttcattc ctggataacc
     2041 gcagattcca tgccttattc aaaagcttaa gggtaagtgc ggcatctgcg ctcccccctg
     2101 caagccccgc tgcaataggt attgtttttt caatatttat cagtgcgcct tccttgacgt
     2161 tgtattttat ttttaggtat tcggcagctc tatatactat attcccttta tctgccggca
     2221 atctgtcatt atttgaagta acttttatgt cacctttcaa tgccctgacg gttatggtat
     2281 caaacaatgc caccgattgc attatcatca atacatcatg atagccgtca ggcctttttc
     2341 taagtacatc aattgcaaga tttatttttg cagctgccct tcctgccagt gtattcaata
     2401 caatcacgct ccgttgcttt atgtatacaa aaatcccccc attatcatta tataccattt
     2461 tctaatttct tgtacatttt tttaccgata agtttgggaa taaaaataaa agcaggaatt
     2521 attcatcctg ctaataaagt ctctccataa ttataaactg ttatccttcc ccatccgaat
     2581 attcttcaat atcattatct tagttccatt tgccactttg cttgggtttt caatattatt
     2641 cagttttgcc agggcatcca ctgtggtatt gtatctcttg gctatgctcc acagcgtatc
     2701 acctttctga accatgtata tagtcactgc cggtatcctg ccatagtcta tactaatgcc
     2761 ttcaactgcc tcaacgttct ctaccagctt tttttcaata tgcctcataa cctcggcata
     2821 aactccaagc accaccctta attcaatcat ttcattgttt atcttgctga agtttacatt
     2881 gtctgcactg cattttacgg tacacggcat accaagcctc acaccgggta tatccatgaa
     2941 atgcctgaag ggaatctgct cggtcatact gcacataggc tccgcactgt atgaagactc
     3001 atagacaaac gtgcactccg ccataccctc cagcagtact ctgtcatcca gcagcttcac
     3061 ttcatttact attggcagca catttacgca gcataccttt tcaatctcag gatccccatg
     3121 cttaataccg acggtttcct tgattacaat gtttgaacgg ccttttccga caaattcgct
     3181 gagtgagaat atatttcttt ctatatctat cacattggtc gggctataag catccgccac
     3241 tacttcctgt tcgatgctgt taaataccct tgctcccata cccagcatga cataggcatt
     3301 gattactttt ctctcgccat tggcatcctc attaacctcc agacggcatt cctgaagagt
     3361 gctttctgtc acacactcca tgtttttctc cgccgcaggg atctctatgt attgagtaaa
     3421 cggcacttca aattcctggt actgcaattg gttttcctca gtctcagact tatatagtat
     3481 gtctattccc agcagtccgt tcacctcgac cttgccttcg attggttttt catcctttat
     3541 aattaccttg ctgtccgatc ttaatatctt gcctatcgca ggtgcatcag ctgccaggtt
     3601 gagttcctca ttgaactcat aatggtcttt gttatagccc atcacttgtt tgaagctgct
     3661 ttcctcccgg agcacctgga tgtctgataa accccttacg tcagaagcca gctcaagatc
     3721 gaaaagatcc tcaaccttgc agtgcatatc catgattacc ttcacgccaa gctttcttga
     3781 gtttatcata taacaatcaa catgctggat tactgtattt atgctttctc tcatcctggg
     3841 ccttgcgcca ggaacttcaa taccctgtga aaaattggcg gccacgtcca tactgttaag
     3901 cggttttcct tcatcatccg ctgcatataa tatacttaat aatatttgac catttacaac
     3961 aaccttgtcg tttaacactt cacaatccct tatctgtaca gttgcacccg gggcaagtat
     4021 cttgtataaa tccggattta tatccggtac atttatatct tcttcgacca ctgtgctgaa
     4081 tacattttcg cctactattc tggaaacctt gagtgggttt tttattagtt caagtggcat
     4141 tattctccgc ctcctactat agtttttcta ttaatttata tgagacgaga aggaatttat
     4201 gccaacattt ttattttagg ggctgttgcg caatatatga agaatg
//
LOCUS       DAOQUR010000107         4107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_24408_length_4107_cov_11.529615, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000107 DAOQUR010000000
VERSION     DAOQUR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24408_length_4107_cov_11.529615"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..349)
                     /gene="rpoZ"
                     /locus_tag="PKI50_12125"
     CDS             complement(101..349)
                     /gene="rpoZ"
                     /locus_tag="PKI50_12125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNT04487.1"
                     /translation="MINPTTNELLKKVDSAYTLCNVVSKRARQLVEGAHPLTECNSGK
                     PVSIAAKEVCEGKVTYDRIKSVPKKKRIIDESMFESGN"
     gene            complement(663..1139)
                     /locus_tag="PKI50_12130"
     CDS             complement(663..1139)
                     /locus_tag="PKI50_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04488.1"
                     /translation="MEFLRYTHLLYFLATWIYVFIFIKPKRIKELIPVAVLGFIILST
                     VDFYIVSLGLYQYNKPLFNIFGAPLFHLLWGGGAAIVYMHYMKQGFTRKFVNVVLFTM
                     ITLAFEFISEKAGVAQRLGKYDFLHSALLDFAALVVLLWVAEGLYGNRIYRDWKDW"
     gene            complement(1265..3289)
                     /locus_tag="PKI50_12135"
     CDS             complement(1265..3289)
                     /locus_tag="PKI50_12135"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNT04489.1"
                     /translation="MVKNYEISEAMTVKLDNWLPDMPKFAEGIRRAPNRGFTLTPDQA
                     EIALKNALRYIPEELHEKLAPEFMEELVTRGRIYGYRFRPEGRIYGKPIDEYKGNCIE
                     GKAFQVMIDNNLDFEVALYPYELVTYGETGQVCQNWMQYRLIKKYLEVMTDHQTLVVE
                     SGHPLGLFHSKPTDPRVVITNALMVGMFDNQKDWHYAMQMGVANYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRKKLGIAPEEDLRGYLFVTSGLGGMSGAQPKAAEIANGVGIVA
                     EVDYSRIETRLKQGWISRVSSDLKEVFSIAEDYLKKKDKISIAYHGNIVDLLEYAVNN
                     DIKIHLLSDQTSCHAAYDGGYCPQGLTFEERTRMLKEDREQFCKLVDKSLRHHFQLIR
                     KLADKGTYFFDYGNSFMKAVYDAGAKEIAKNGVDEKDGFIFPSYVEDIMGPELFDYGY
                     GPFRWVCLSGKHEDLIKTDHAAMECIDPARKAQDRDNWVWIRDAEKNKLVVGTQARIL
                     YQDAWGRTKIALKFNEMVRKGEIGPVMLGRDHHDVSGTDSPFRETSNIKDGSNIMADM
                     AVQCFAGNAARGMSLVALHNGGGVGIGKAINGGFGLVLDGSERVDEIIKDAMLWDVMG
                     GVARRSWARNENSVTTSIEYNKSQEGKDHITLPFISDDGLIRETVKKFLG"
     gene            complement(3431..3922)
                     /locus_tag="PKI50_12140"
     CDS             complement(3431..3922)
                     /locus_tag="PKI50_12140"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HNT04490.1"
                     /translation="MYKINCESMGDVKKYIWYYRKDNSEHGPYTYEDIVEMVREGVIG
                     PEDYVLKFGNRKFIKVSEMQGLSEIQGPPEMQKLPEMQEPSEAGEKPEETKEEQHEGM
                     KEAAPEIKKEKAKEEHHIAHDNRAKYIQGKHKKESFSPKLPVIIACFVGLCFIVVLWI
                     LLS"
BASE COUNT         1071 a         1048 c          661 g         1327 t
ORIGIN      
        1 tggtctcgaa ctgactgatt taaatagtac catatggtcc ttctatatgc aattgaaaaa
       61 tatctgttgc catacatgtc ctaagaaacc tgcagtaatt ttaattgcct gattcaaaca
      121 tactttcatc tataatacgc ttctttttag gaacgctttt gattcgatca taagtaactt
      181 tcccttcgca aacctcttta gctgcaatag aaacaggttt tccagaatta cattctgtaa
      241 ggggatgagc tccctctacc aattgtcttg ccctcttgct tactacatta catagtgtat
      301 atgcgctgtc aactttcttc aacaattcgt ttgttgttgg atttatcata atcaatttct
      361 ccccttctcc aataatctcc ataaatgcta ttccatatga tactatgctt ttatatttca
      421 acattcgcat caataggcac actttttaaa ttagttatta tcccgcggac ttcactttct
      481 ttttcactaa catttagctg cccgtccaaa ctaaaattac ttccagtggt cttatccctc
      541 caacttcttt tctgatgcaa ggtgtaaaaa aatgttagca ctcacacgtt ttgagtgcca
      601 acatttatat cataccagtt tgtctttgtt gagtcaatat atttctgaaa tttcagacac
      661 ttttaccaat ctttccaatc tctatatatc ctgttgccgt acaaaccttc ggcaacccat
      721 aacagcacta ccaacgcagc aaaatccagc aacgcgctgt gaaggaagtc atatttcccc
      781 aaccgctgag ccacgccggc cttttccgat ataaattcaa atgccaatgt aatcatcgta
      841 aacagcacta catttacgaa tttacgggta aacccctgtt tcatatagtg catatatact
      901 attgcggctc ctccccccca cagcaaatga aataaggggg ccccgaaaat attgaacagc
      961 ggtttgttgt actggtataa gccaagggaa acaatataga agtcaaccgt actcaggata
     1021 ataaacccca atacggctac aggaatgagt tccttgatgc gttttggctt aatgaatatg
     1081 aacacataaa tccaagtggc caaaaaatac aacaaatgcg tgtaccttaa aaattccata
     1141 ctctcacctc acaaatattg tgaggtactt ttgcataaac tatacagtaa gctgatagtg
     1201 taaaaaacaa cacagaaaag ctgctgcaaa acgagagtct gcagcagctc ctttttacgg
     1261 tttgttatcc caaaaacttt ttcacagttt ctctaatcaa gccatcgtca gatatgaagg
     1321 gcagtgttat atgatccttc ccttcctgag atttattgta ttcaatactt gttgttacgg
     1381 aattctcatt tcttgcccag gaccttcttg caaccccgcc cataacatcc caaagcatcg
     1441 cgtcctttat tatttcatca acacgttcgc ttccgtcaag cacaagtccg aagcctccgt
     1501 ttatcgcctt tcctataccg actccgccgc cgttgtgaag agcgaccagg ctcatacctc
     1561 ttgcggcgtt tcctgcaaaa cactgcaccg ccatgtccgc cattatattg cttccgtcct
     1621 ttatattgga agtctccctg aagggggagt cggttccgct tacatcatga tggtcacgtc
     1681 ccagcattac aggccctatt tccccttttc tgaccatttc attaaattta agcgctatct
     1741 tggtccttcc ccatgcatcc tgataaagga ttcttgcctg ggttccgaca accagcttgt
     1801 tcttttcggc atctcttatc catacccaat tgtccctgtc ctgggctttt cttgcagggt
     1861 caatacactc catcgctgcg tgatccgtct ttatcagatc ctcatgcttt ccgctcaggc
     1921 atacccacct gaaaggccca tagccgtaat caaaaagttc ggggcccatg atatcctcaa
     1981 catatgaagg gaatatgaat ccgtcttttt catcaacacc gttctttgct atttcctttg
     2041 ctcctgcatc atacaccgct ttcatgaagg aattgccgta atcgaagaaa tatgtccctt
     2101 tatccgccag cttccttata agctggaagt gatgcctcaa gctcttatcc accaatttgc
     2161 agaactgctc cctgtcttct ttaagcatcc tggttctttc ttcaaaggtc agtccctggg
     2221 gacagtaacc gccgtcatat gcggcatggc atgaggtctg gtccgacaga aggtgaatct
     2281 ttatatcatt gttaactgca tattcaagca aatctactat atttccatga tatgcaattg
     2341 atattttatc ttttttcttc aggtaatcct ctgctatgct gaatacttcc ttaagatcag
     2401 aggaaactct ggatatccaa ccttgcttaa gccttgtttc tattcttgaa tagtcaacct
     2461 cagcaactat tcctactccg tttgctatct ccgccgcctt tggctgagcg ccgctcatgc
     2521 ctccaagccc cgaggttacg aaaagataac ctctcaaatc ctcttcgggt gctatgccga
     2581 gcttttttct tcccgcatta agtattgtgt tgaaggttcc gtgcactatc ccctgtggcc
     2641 cgatgtacat ccagccccct gctgtcatct gcccgtagtt tgcaactccc atctgcattg
     2701 catagtgcca gtccttctgg ttgtcaaaca ttcctaccat tagtgcattg gtaatgacca
     2761 cccttggatc cgtaggcttt gaatgaaaca accctaaggg atgccctgat tccacaacaa
     2821 gggtctgatg atcggtcatt acttcaagat atttctttat cagcctgtac tgcatccagt
     2881 tctggcatac ctgccccgtt tctccgtagg ttaccagctc ataaggatat aaggctactt
     2941 caaagtccag gttattgtct atcattactt gaaaagcctt tccttctatg cagtttccct
     3001 tgtattcgtc tataggcttt ccgtaaatcc tgccttccgg cctgaaacga taaccataga
     3061 ttcttcccct tgtaacaagc tcttccataa actcgggagc aagcttttca tgaagctcct
     3121 caggtatata tctgagggca tttttcaatg caatctccgc ctgatcagga gtcaatgtaa
     3181 agcctctgtt cggtgccctt ctgatgcctt ctgcaaactt gggcatatcc ggcagccagt
     3241 tgtccagctt aactgtcatt gcctctgaaa tctcataatt cttaaccaat ttaattcccc
     3301 caatgcctga ttatactatt attctatctt attatagtct attaattgca caaaatatta
     3361 aattcagtat agtaattatt ccgcttcttt tttcagaata aaaaaccttg aaattcaagg
     3421 tttgaaaaaa tcaagacaga agtatccaca aaactacaat aaagcataag cctacaaaac
     3481 aagctataat taccggtaat tttggactga aggattcttt cttgtgtttc ccctgaatat
     3541 acttggctct attgtcatgt gcaatatggt gctcctcttt tgctttctct ttctttattt
     3601 caggagccgc ttccttcatt ccctcatgct gctcttcctt cgtctcttca ggtttctcac
     3661 cggcctcaga aggttcctgc atctcaggaa gcttttgcat ttcgggaggc ccttgtatct
     3721 cagaaagccc ttgcatttca gataccttga tgaacttcct gttaccaaac ttcagcacat
     3781 agtcctcagg cccgattacc ccttctctga ccatctcaac aatatcctcg taggtataag
     3841 gcccatgctc gctattgtcc tttctgtaat accatatgta cttttttaca tcccccatgc
     3901 tttcacagtt gattttatac attagcttca cctgccaaac ttaacatttt gtatactcca
     3961 gttaaattat accattaata aacaaattct gaaatctttt gttttaataa tatcacaaat
     4021 ttcgactatt atgataccgc caaaaataaa accgacggtg tcaatttgtt ctcggttttt
     4081 aaaaagcaga ttctcccttt atggttt
//
LOCUS       DAOQUR010000108         4034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_25057_length_4034_cov_11.898970, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000108 DAOQUR010000000
VERSION     DAOQUR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25057_length_4034_cov_11.898970"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..569)
                     /locus_tag="PKI50_12145"
     CDS             complement(156..569)
                     /locus_tag="PKI50_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT04491.1"
                     /translation="MNIAFFLTPKEEVIWISADSTMRQAIEKMEFHRYSALPLVDSGG
                     KYAGTITEGDILWHLKHSKNLEFKDTNKIMIRDISKYQNNKPVSIESHIEDLILLSIN
                     QNFIPVVDDHDIFIGIIKRSVIINYFYKLLLNRKI"
     gene            816..2339
                     /locus_tag="PKI50_12150"
     CDS             816..2339
                     /locus_tag="PKI50_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT04492.1"
                     /translation="MMRVNINRNSDTPVYLQIKNQIRDMIISGELPAGHILPPERILS
                     ELLNVNRTTVAKAYHELKADGLVQARVGKGTMVAVKHFKGRFNNIRQFNPIPWYQFYN
                     ESVDSGGDHIISDIMDINCTEDIISFSGGFPSPELYPLEELSSIQEYLFKTVGKQLLL
                     HSPVEGLTSLRAGISSLMKSRNINISPKDIMIVSGSQQGLDYIARVFIKPGDTVILEE
                     PSFFGAMQIFRSAGARIIGAPVDSCGMKTDMLESLIQRYKPKFIYALPTFQNPSGIVM
                     SLERRYELLDLSYKYRVPIVEDDPYGDLRYEGKAIPSLKALDPYEHVIYLSTFSKVLS
                     LGLRIGWVAAPQQVIRKFAYFKQITDMHVNTPGQYLISEFLSQGCYEKHLNAVLTEYR
                     KKRDLMNAALEKYKLPEVLWEKPEGGYYIWCRLPEYVTNNRLVSKAAEKGVVYLPGEA
                     FYPDGTQGENYMRLNFTFPNSNKIEEGIKLLMDALTEVEVRDGSNSAGRSYSRSPIV"
     gene            2370..3263
                     /locus_tag="PKI50_12155"
     CDS             2370..3263
                     /locus_tag="PKI50_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNT04493.1"
                     /translation="MEIKIKQVDAFTGIPFGGNPAGVVTDAAGLSDELKQKIAREMNL
                     SETAFVSPSKAADFKVQFFTPRFEVDLCGHATIGTFSALHEEGKLPSDKNIFYQETKA
                     GILPVELTSINNEKVFMMTQALPKFENIDLSKSEIAGLLGLSKDDLMDTPDLMVNTGI
                     WWLVFGVKTLDKLMNAKPDLKTIERISEQYNIVGITPFCLEALDPKYDYHLRAIAPFV
                     GVAEDPVCGTGNGCVSSYIVQNGLVKCDDTVNMIGEEGQEVNRPGCVYASISKKEGEI
                     SRVQIGGTAVTILQGVIKITD"
     gene            3282..3971
                     /locus_tag="PKI50_12160"
     CDS             3282..3971
                     /locus_tag="PKI50_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNT04494.1"
                     /translation="MKTIGIIGGVSWESTVEYYRILNETVNKKLGGLHSAKCLIYSVE
                     FQEITDLMQTGDWKAISVRMQEIASILKSGGADFLIICSNTLAAPAPEIEKNIGMPVH
                     SIAEATAKEILGMKLKKIGLLGTKYTMEGDYYRNTLYKYGIETVVPEKEDREAINSII
                     FDELCLGILKESSKEIFRNIISKLQSKGAEGIVLGCTEIPLLVKQEDVSIPVFDTLTI
                     HAVSAAELALG"
BASE COUNT         1223 a          831 c          940 g         1040 t
ORIGIN      
        1 gacatgggga cgtggggaca cggggacgtt tcgcgcgggg acacggggac gtttcccctg
       61 tcccgctttc agtgggacac gcgaaacgtc cccgtgtccc cgcgtccccg tgtcgcatta
      121 ttctattttt atgcgaaaac acttctcttc ccgatctaga ttttcctatt cagaagcaat
      181 ttgtagaagt aattgataat gacgctcctt ttgattatcc cgataaatat atcatggtca
      241 tccacaacag gtatgaagtt ctgattaatc gagaggagaa tcaaatcttc tatatgtgat
      301 tcgatggaaa ccggcttatt attctggtat tttgaaatat cccttatcat aatcttgttg
      361 gtgtctttaa actccagatt ctttgaatgc ttcaaatgcc acagtatatc cccctctgtt
      421 attgtacctg catatttgcc gccgctgtca acaagaggca aagcgctgta tctgtgaaat
      481 tccatctttt caatggcctg tctcattgta gagtccgctg atatccagat gacttcttct
      541 ttcggtgtta gaaaaaaggc aatattcatt gatatcctcc tgacttccct tggatatttt
      601 tttccggcta attatattat aacgcatcgc aggaaatttt tcgattgcag aaaaaatttt
      661 tacctctgcg tgttacaact atttggatta ttccaatcaa aaattggatg attaataaat
      721 atcagaattg ggggattttt tactgtaaaa tatctgctat aataataatt ggatgatgcc
      781 aattcaataa tacatttaca agagagaaga gtgatatgat gcgcgtcaat attaaccgga
      841 actctgatac ccctgtttat ctgcaaataa aaaaccaaat ccgtgatatg ataatctccg
      901 gtgaactgcc tgccggccac atacttcccc ctgaaaggat tttaagcgag ctgcttaatg
      961 tcaacagaac aactgttgca aaagcatatc acgagctgaa ggccgacggg ctggtacagg
     1021 cacgggtcgg taaaggtacc atggttgcag tcaaacactt caaagggcgt ttcaataaca
     1081 tccggcagtt caaccccatt ccctggtatc aattctacaa tgagagcgta gattccggcg
     1141 gcgaccatat aataagcgat atcatggata ttaactgcac cgaagatatc atttcatttt
     1201 ccggcggttt cccttcgcct gagctttatc ctttagagga actgagcagc atccaggaat
     1261 accttttcaa aactgttggt aagcagcttt tactccacag cccggttgaa gggctgacct
     1321 ccctgagggc aggcatcagc agcctgatga aatcaagaaa tatcaatatc agcccaaagg
     1381 atataatgat agtatccggc tcccagcagg gcctggatta cattgcccga gtcttcatta
     1441 agccggggga tactgtaata cttgaggagc cttctttctt cggtgcaatg cagattttcc
     1501 gctccgcggg agcaaggatt atcggcgcac ctgtggacag ctgcggaatg aaaacagata
     1561 tgcttgaatc gcttatacaa aggtacaaac cgaagttcat ttatgcactt cctacttttc
     1621 agaatccttc aggaatagta atgagccttg agcgcaggta tgaactgctg gacttgtcct
     1681 ataaataccg agtccccata gtagaggatg acccctacgg tgatctgcgt tatgaaggaa
     1741 aagccatacc atccttgaag gctttggatc cctatgaaca tgtaatttat ttaagcacat
     1801 tttccaaggt tctttccctt gggctgcgca tcggctgggt tgctgcacct cagcaggtca
     1861 tcagaaaatt tgcatacttt aagcaaatta cggacatgca cgtaaataca ccgggccagt
     1921 acttgataag cgaattcctt tcccaggggt gttatgagaa acatcttaat gcagtgctga
     1981 ctgaatacag aaaaaaaaga gatctgatga atgcagcact ggagaaatac aaactgcccg
     2041 aagttctctg ggagaagccg gaaggcgggt attacatctg gtgccgcctg ccggagtatg
     2101 ttacaaataa cagactggtt tcaaaagctg cggaaaaagg agtggtatac ctgccgggcg
     2161 aggcctttta cccggatggt acacagggag aaaactatat gaggctcaac tttactttcc
     2221 cgaattcaaa taaaattgaa gaaggaataa aactgctaat ggatgccttg acagaggttg
     2281 aggtaagaga cggatcgaac tccgcgggca gaagctattc aagaagcccg atagtgtagg
     2341 attgaaaata aaaatgagga gggttttatt tggaaataaa aattaaacag gtggatgcct
     2401 ttaccggaat accctttggg ggaaaccccg ccggggtggt tactgatgcc gcaggcttaa
     2461 gtgatgaatt gaaacagaaa atcgccaggg aaatgaatct ttcggaaact gcattcgtat
     2521 ccccttcaaa ggccgccgat tttaaagtgc aattcttcac acccagattt gaagtggact
     2581 tatgcggcca tgcaaccata ggcacttttt cggctcttca tgaagaagga aagcttcctt
     2641 ccgataagaa catattttac caggaaacaa aagccggaat cctgcctgtg gaattaacaa
     2701 gcatcaataa tgaaaaggta ttcatgatga ctcaggcatt gccgaaattt gaaaacattg
     2761 atctgagcaa aagtgaaatt gcaggactcc taggcttgag taaggatgat cttatggaca
     2821 caccggattt aatggtaaac accgggatat ggtggctggt attcggagtt aaaacactgg
     2881 ataagcttat gaatgcaaag cctgatttaa aaaccattga gagaataagc gagcaatata
     2941 acattgtcgg tatcactcct ttctgtctcg aagcactgga cccgaaatat gactatcacc
     3001 tgagagcgat tgctcctttt gtcggagttg ccgaagatcc tgtatgcggt accggcaacg
     3061 gatgcgtatc ttcatacata gttcagaacg ggctggtaaa gtgtgatgat actgtaaata
     3121 tgatcggaga ggaagggcag gaagtcaaca ggcccggctg cgtatatgca agcatttcca
     3181 agaaggaagg ggaaataagc cgggtacaaa tcggaggtac agcagttaca atactgcagg
     3241 gagtaataaa aatcacagat tagaaagggg gaggatattc aatgaaaact atcggaataa
     3301 tcggcggagt aagttgggaa tctactgtcg agtattacag gatactgaat gaaaccgtaa
     3361 ataaaaaact gggaggactc cattccgcca agtgcctcat atattctgta gaattccagg
     3421 agataacgga tctgatgcag accggggact ggaaggctat ttcagttcgg atgcaggaga
     3481 tagcaagtat actgaagtca gggggagcag acttccttat catatgctca aacactcttg
     3541 ctgcaccggc tccggaaata gaaaaaaata tcggtatgcc tgtgcacagc attgccgaag
     3601 cgacagcaaa ggaaatactc ggaatgaagc ttaaaaaaat aggcttgctg ggaacaaagt
     3661 ataccatgga gggtgattat tacaggaaca ctctttataa atacggtata gaaaccgtag
     3721 taccggagaa agaagatagg gaagcaatta actcaataat atttgatgag ctttgtcttg
     3781 gcatattaaa ggaatcctcc aaggaaatat tcagaaatat cataagcaag cttcagtcaa
     3841 aaggcgctga gggcatagtc ctcgggtgca ctgaaatccc cttgcttgta aagcaggagg
     3901 atgtaagcat accggtattt gatacactga ctatccatgc ggtttccgca gcagagctgg
     3961 cactaggcta aggaacaaaa aggacacggg gacgtttctc ctgtcccgct ttcagcggga
     4021 caggagaaac gtcc
//
LOCUS       DAOQUR010000109         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_27497_length_3772_cov_12.585687, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000109 DAOQUR010000000
VERSION     DAOQUR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27497_length_3772_cov_12.585687"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PKI50_12165"
     CDS             <1..134
                     /locus_tag="PKI50_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HNT04495.1"
                     /translation="FAGFSAGANLAAALKLLKVDFKGKNIVILLPDSGTKYLSTDLW"
     gene            complement(158..1366)
                     /locus_tag="PKI50_12170"
     CDS             complement(158..1366)
                     /locus_tag="PKI50_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNT04496.1"
                     /translation="MSNFAQRMTAMEKSAKIIRNLFGAMNDPGIISFGGGAPAKEALP
                     IDIVREIANEVIRADKRGAEALQYGPVMGAADLREAVVNDLLAPKGVAASVDNIMITS
                     GGLETMNLMCQLFIDPGDVILVESPSFVHCVEIFEMFQANCIAVDMDDNGMIPEDLEA
                     KILKYRPKMVYVIPTFQNPTGRTLSLDRRQKIAEMGSRYDVIILEDDPYRDIRYSGSD
                     LLPIKAFDQTGHTVLANSFSKIFSPGSRLGYALANDEIISRLFDAKTATNSHTSMLPQ
                     VICAEFFKRGYYPAHHKMICDLYRERRDIMIECIDKYFPEGTKRTFPDGGLFTWAELP
                     EGINTTELLAEAVTNPDVKVAYVAGEGFFTEGNGAGSDCMRISFGSAAPERIRTGIER
                     LGRLICSKLK"
     gene            complement(1422..1910)
                     /gene="ybaK"
                     /locus_tag="PKI50_12175"
     CDS             complement(1422..1910)
                     /gene="ybaK"
                     /locus_tag="PKI50_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNT04497.1"
                     /translation="MEKVKTNAMRLLEKAGIKYNTYAYEHSDGLIDGVSVAGKIGCPV
                     EKVYKTLVAQGTSREFYVFIIPVAAELDLKAAARSVGEKAVEMIKVADINKVTGYIRG
                     GCSPLGMKKQYKTVIDSSCKAMDTIIVSAGKIGHQIELAPEDLIKLTGCRTEAVCARG
                     NL"
     gene            complement(1966..>3772)
                     /locus_tag="PKI50_12180"
     CDS             complement(1966..>3772)
                     /locus_tag="PKI50_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HNT04498.1"
                     /translation="RDIQKAGEEALSYIRANGLKGIVLAGRPYHIDPQINHGIPEMIN
                     SLGMAVLTEDSVAHLGRVERPLRVVDQWAYHSRLYAAASFTAKQEDVELVQLNSFGCG
                     LDAVTTDQVQEILNENSKIYTTLKIDEGNNLGAARIRLRSLKATILERHKNGFELKKV
                     DNRYKRIEFTEDMRTKHTILVPQMSPIHFQFLEKAFRLSGYNMVVLASMDKGAVDEGL
                     KYVNNDACYPAIIVIGQLIEALKSGKYDLNNTSVMISQTGGGCRATNYIGFLRKALKD
                     AGFEKVPVISLNAVGMEKNSGFKFTTELLNRAMMGLVYGDLLMNVLYRVRPYERIKGS
                     ANMLYEKWAEICKESVEYASRKVFKQIIRQIVKEFDELEMINQAKPKVGLVGEILVKF
                     HPTANNNVVNIVEAEGAEAVVPGLTDFLLYSAYGLEYRYKYLSGSKKSQLFGRAAVMI
                     IEFYRKAYREALSASKRFYASQTIEEIAEGASSVVSLGHQTGEGWLLTGEMVELINNG
                     VRNIICMQPFACLPNHVTGKGMMKELKRVYPGTNIVAIDYDPGASEVNQLNRIKLMIS
                     AAFENMNKFEAHKVGSYVTTDYRRMRSTLIMRTRL"
BASE COUNT          953 a          981 c          716 g         1122 t
ORIGIN      
        1 tttttgcagg cttctcagcc ggggcgaatc tggcagcggc actaaagctg cttaaggtag
       61 attttaaggg aaaaaacatt gtaatattgc tgccggactc cggcacaaaa tatctgagca
      121 cagatttgtg gtaagaatac gtcgcattct tcagtgttta tttcaatttg gagcaaatca
      181 gcctgcctaa cctttcaatg cctgttctga ttctttccgg agcagcactg ccgaaactga
      241 tcctcataca gtcgctgccc gcgccattac cttcggtaaa gaagccttcc cctgccacgt
      301 aggcaacctt gacatcggga tttgtaacag cttcagccaa cagctcagtg gtattgatgc
      361 cttccggcag ctccgcccat gtgaacaggc ctccgtccgg gaaggttctc ttggtccctt
      421 cgggaaaata cttatcgata cattcaatca tgatgtctct tctttcgcgg tacaaatcac
      481 atatcatctt atgatgggcc ggataatacc cgcgtttgaa gaattcggcg catattacct
      541 gaggcagcat ggatgtgtgg gaattggttg ccgttttggc atcaaacaat cttgatatta
      601 tttcatcatt tgcaagagca tagcccagcc tgctgcccgg cgagaatatc ttggagaagc
      661 tgtttgccaa gacagtgtga ccggtctggt caaaggcttt tatcggcagc aagtcgcttc
      721 cgctgtaacg aatgtcgcga tagggatcat cttccagaat aatcacatca taacggcttc
      781 ccatttcagc gattttctgc cttctgtcca aggacagggt tctgccggtt gggttctgga
      841 atgtgggaat aacatacacc attttgggtc tatactttaa tatttttgcc tccaggtctt
      901 caggaatcat gccgttatca tccatatcca cagcaatgca atttgcctgg aacatttcga
      961 atatttcaac acaatgtaca aaggaaggtg attcaaccag aatcacatca ccgggatcaa
     1021 taaacagctg gcacattagg ttcatggttt caaggccgcc gctggtaatc ataatattat
     1081 caacacttgc agcaactccc tttggtgcca gcaaatcatt aacaacagcc tcccgaagat
     1141 ccgccgcacc cataacggga ccgtactgaa gagcctccgc gcctcttttg tcagctctaa
     1201 tcacttcatt tgctatttca cggacaatat ctatgggaag tgcctctttt gccggagcac
     1261 cgcccccaaa ggaaatgata ccggggtcat tcatagcacc aaaaagattt cggataatct
     1321 ttgcggattt ctccattgca gtcatacgct gcgcaaaatt tgacacaata acctctcctt
     1381 ttaattttat atattagtat caacatatca tttgatttca gctacaaatt tcctcgtgca
     1441 caaacagcct ctgtcctgca gccggtcagc tttattaaat cctccggggc taattcaatc
     1501 tgatgtccta tctttcctgc gcttacgatt attgtatcca tggctttaca ggagctgtca
     1561 atcactgtct tatactgctt tttcatgcct aggggagagc atcccccgcg aatatatccg
     1621 gtaactttat tgatgtccgc gacttttatc atctcaacgg ctttctcccc cacagacctt
     1681 gccgctgctt tcaaatcaag ctcagcggca acagggatta taaaaacata gaattccctg
     1741 cttgtcccct gggcgacaag ggttttgtac actttttcaa cagggcagcc gattttaccg
     1801 gcaactgaaa ctccgtcaat caagccgtcg ctgtgttcat aagcatatgt attatacttg
     1861 attcctgcct tttcaagaag ccgcattgca tttgtcttta ccttttccaa ttctacccct
     1921 cactccatga atatcgtgaa caaaggaagc tgaactaaag tgttcctaga gcctggttct
     1981 cataatgaga gttgaacgca tccgtctgta atctgttgtt acataggagc ctactttatg
     2041 agcctcaaac ttattcatgt tttcaaatgc tgcggaaatc ataagtttta tcctgttcag
     2101 ctgattcact tcacttgcac ccgggtcata atcaatagct actatgttgg ttccggggta
     2161 aacccgtttc agctccttca tcattccttt tcccgtgaca tgattcggaa ggcatgcaaa
     2221 gggctgcatg cagattatat ttctgactcc gttattaata agctcaacca tttcccctgt
     2281 caaaagccat ccttccccgg tctggtggcc taaagacaca actgatgagg caccctccgc
     2341 tatctcttct attgtttggg aagcataaaa ccttttgctt gcagatagcg cctccctgta
     2401 agccttcctg tagaactcaa taatcataac ggctgcacgc ccaaagagct gggatttttt
     2461 gctccctgac aaatatttat atctatactc caaaccatag gcactataca gcagaaagtc
     2521 cgtaagtccc ggcactactg cttccgctcc ctcggcttca actatattga caacattatt
     2581 gtttgccgta ggatgaaact tgacaagtat ttccccaacc agcccaacct ttggcttagc
     2641 ttgattgatc atttccaatt catcaaattc tttaacaatt tgtctgataa tctgcttgaa
     2701 tacttttctg ctggcatatt ccacagattc cttacatatt tcagcccact tctcatacag
     2761 catattggcg gagcccttaa tcctttcata gggcctgact ctgtacagta cattcattag
     2821 caaatcgccg tatacaagtc ccatcatggc tctgttcaaa agctccgttg tgaacttgaa
     2881 gcctgaattc ttctccatac ctacagcatt caacgatata acgggaactt tctcaaaacc
     2941 cgcatcctta agggcttttc tcaggaagcc tatataattg gtagccctgc agcccccgcc
     3001 tgtttgagat atcattaccg aagtattatt caaatcatat ttgccggact tcagtgcttc
     3061 tataagctgt cctattacaa taatagcagg gtagcaggca tcattattca catacttcag
     3121 gccttcatcc acagctcctt tatccattga tgccaaaaca accatattat aacctgatag
     3181 tctgaacgcc ttctccagga actgaaagtg tatcggagac atttgaggca ctaaaatagt
     3241 atgcttcgtt ctcatatcct ctgtgaattc aattctctta tatctgttgt caactttctt
     3301 aagctcaaac ccgttcttat gcctctccaa aattgtagcc ttaagtgacc gaagccttat
     3361 cctggctgct cccagattat taccctcgtc tattttcagg gttgtataaa tcttggaatt
     3421 ctcattaagt atttcctgca cctggtctgt tgtaacagca tccagcccgc agccgaagga
     3481 attcaactgg accagttcca cgtcctcctg ctttgcagta aagcttgccg cagcatacag
     3541 cctggagtgg tatgcccatt gatccactac tctgaggggc ctttcaaccc gtcccagatg
     3601 ggctacggag tcttccgtca atacagccat gccaagggaa tttatcatct ccggtatgcc
     3661 gtggtttatc tgagggtcta tatggtacgg ccttccggca agcactatcc cctttaatcc
     3721 attagccctg atataactca aggcttcttc tccggccttt tgaatatccc tc
//
LOCUS       DAOQUR010000110         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_27714_length_3749_cov_10.949377, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000110 DAOQUR010000000
VERSION     DAOQUR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27714_length_3749_cov_10.949377"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..294)
                     /locus_tag="PKI50_12185"
     tRNA            complement(218..294)
                     /locus_tag="PKI50_12185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Arg,seq:tct)
     gene            complement(301..374)
                     /locus_tag="PKI50_12190"
     tRNA            complement(301..374)
                     /locus_tag="PKI50_12190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(340..342),aa:Gly,seq:tcc)
     gene            complement(381..464)
                     /locus_tag="PKI50_12195"
     tRNA            complement(381..464)
                     /locus_tag="PKI50_12195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(428..430),aa:Leu,seq:tag)
     gene            complement(471..546)
                     /locus_tag="PKI50_12200"
     tRNA            complement(471..546)
                     /locus_tag="PKI50_12200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Lys,seq:ttt)
     gene            complement(557..632)
                     /locus_tag="PKI50_12205"
     tRNA            complement(557..632)
                     /locus_tag="PKI50_12205"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:Gln,seq:ttg)
     gene            complement(643..719)
                     /locus_tag="PKI50_12210"
     tRNA            complement(643..719)
                     /locus_tag="PKI50_12210"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:His,seq:gtg)
     gene            complement(729..805)
                     /locus_tag="PKI50_12215"
     tRNA            complement(729..805)
                     /locus_tag="PKI50_12215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(769..771),aa:Arg,seq:tct)
     gene            complement(812..885)
                     /locus_tag="PKI50_12220"
     tRNA            complement(812..885)
                     /locus_tag="PKI50_12220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(851..853),aa:Gly,seq:tcc)
     gene            complement(986..1582)
                     /gene="rdgB"
                     /locus_tag="PKI50_12225"
     CDS             complement(986..1582)
                     /gene="rdgB"
                     /locus_tag="PKI50_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNT04499.1"
                     /translation="MMKMIAATNNSHKLEEIRAILKDKGIEVISLADAGLDIEIEENG
                     DSFMGNALIKAREVFRLTGEAAIADDSGLEVDALKGQPGVKSARYSGESGADKDKKNN
                     EKLKEALKDIPDNERGARFCSAIAAVFPRGKEITAEGYVYGKIGYEEKGEHGFGYDPL
                     FIVDGYGRTMAELDSEEKNKISHRANALKEFVKKLDML"
     gene            complement(1554..2321)
                     /gene="rph"
                     /locus_tag="PKI50_12230"
     CDS             complement(1554..2321)
                     /gene="rph"
                     /locus_tag="PKI50_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNT04500.1"
                     /translation="MQRIGGRNNEQIRPVKIIRNYLKHAEGSVLIEMGDTKVICTASV
                     DDKVPPFIKGTGKGWITSEYGMLPRSTEVRKPRESARGRIDGRTMEIQRLIGRALRSI
                     VNLEKLGEITIYIDCDVIQADGGTRTASITGSYVALVDALYKLVQQERLKEMPLNGYI
                     AAVSVGIKDGEAILDLNYSEDSTCQVDMNLVMTDRGEFIEIQGTGEESPFSKVQLQEM
                     MELAEKGIRELIEIQKRSLGEIADKVGTYDENDSSDK"
     gene            complement(2554..3606)
                     /locus_tag="PKI50_12235"
     CDS             complement(2554..3606)
                     /locus_tag="PKI50_12235"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT04501.1"
                     /translation="MNRNLALLLALLICCTLVLTSCSNPLISLWDFFNKKTDTVEESP
                     QQVQEETQSMSEVSVSQPEDSRATVLYYKDNEDFLIPVMRYMPKGDLGVAKTAISAII
                     YSPETVEDLKAAGLTPTLPMGTKINGAVIRENGLAIIDFSKEFLNFSSKKAEELGIKA
                     VTYTLTEFPNIKSVEIRVDGKKIEEMPKGTEVGSELKRTEINLQPVEKAGEKLSKVVV
                     YYHKKGSGAYSYFVPLTKVVSGFENSVEAALSALLEGPEAGSLKNPFPEGTKLLDVQV
                     NSGIAYVNFSEHILANQGDKAAENAVKKAVALTLKEFPAISKAKIFVNGKIVENTAGV
                     GADEYISVPVFVNFYE"
BASE COUNT          882 a          966 c          718 g         1183 t
ORIGIN      
        1 ctatggtgcg cctgaaggga gtcgtcgcct gctgcgggta aacccttgca tgctagtgac
       61 gtcgcccctt gtcgtttctc tcctgcaggg ctcctacgct cactacaaaa tgcccgcaca
      121 ggggcatttt gcttaacgct cgcgccctct cgggttcgac tccataaatt tataaaataa
      181 aaaaaactaa ctaaaaagct agtttatctt accatcatgg tgcgcctgaa gggagtcgaa
      241 cccccggcac acggattaga agtccgttgc tctatccaac tgagctacag gcgcatattt
      301 tggagcgggt gaagggaatc gaaccctcgc gatcagcttg gaaggctgac gttctaccat
      361 tgaactacac ccgcatacaa tggtgcggga aaagggactt gaacccatac gtcattgaca
      421 ctagatccta agtctagcgc gtctgccaat tccgccattc ccgcatgttt tggtgactca
      481 ccggagaatc gaactccgga caccatgatt aaaagtcatg tgctctaccg actgagctag
      541 tgagtctcaa ataaattggc tggggtggca ggatttgaac ctacgagatg caagagtcaa
      601 agtcttgtgc cttaccgctt ggctacaccc caaagaataa tttggggtgg gtaatcgggg
      661 tcgaaccgac ggcctccaga gccacaatct ggcgctctaa ccaactgagc tatacccacc
      721 atataatatg gtgcgcctga agggagtcga acccccggca cacggattag aagtccgttg
      781 ctctatccaa ctgagctaca ggcgcatatt ttggagcggg tgaagggaat cgaaccctcg
      841 cgatcagctt ggaaggctga cgttctacca ttgaactaca cccgcacgct tgcaatatac
      901 tgtttttagt atcgccgaca attatagatt ataacaaaat ttatattggg tgtcaactga
      961 tttggaagta tatttgccat gcaatttata gcatatccag cttctttaca aactctttga
     1021 gagcatttgc cctgtggctt attttattct tttcttccga gtcaagctcg gccatggttc
     1081 tgccataacc gtcaacaata aagagcggat catatccgaa tccgtgctct cccttttctt
     1141 cataaccgat ttttccgtat acatagcctt ctgcagttat ctcttttccc cgggggaata
     1201 ctgcggcaat tgcggaacag aaccttgctc ctctctcatt atccggtatg tctttcagag
     1261 cttccttaag cttttcattg tttttcttat ccttgtccgc tcccgattcc cctgagtatc
     1321 ttgctgattt cactcccggc tgtcccttca gtgcatctac ctcaaggcct gaatcatcag
     1381 ctatcgctgc ctctcctgta aggcgaaaga cctctctcgc ctttatcagg gcatttccca
     1441 tgaaggaatc cccgttttct tcaatctcaa tatccaatcc ggcatccgcc agggatatta
     1501 cttctattcc cttatccttt aatattgctc ttatttcctc aagcttatga ctattatttg
     1561 tcgctgctat cattttcatc atatgtacct accttatcgg cgatttcacc aagggatcgc
     1621 ttttgtatct ctatcagctc ccgtatgcct ttctcggcaa gctccatcat ttcttgaagc
     1681 tgcactttgc tgaaggggct ttcttctccc gtaccttgaa tttctataaa ttccccccta
     1741 tcggtcatta ccaggttcat atctacctgg caggttgaat cctcggaata gttcagatcc
     1801 agtattgcct ctccgtcctt tatgccaacg cttactgccg caatataacc attcaagggc
     1861 atttctttta acctttcctg ctgaaccagc ttatataaag catcaacaag ggctacgtag
     1921 gatccggtga tggatgctgt tcttgtaccg ccatcagcct gtataacgtc acagtcaata
     1981 taaatcgtta tctctcccag tttttcaaga ttcacgatgg atcgcagcgc ccttccgatc
     2041 agtctctgta tttccattgt cctgccgtct attcttcctc ttgcagactc ccttggcttg
     2101 cgtacttcgg ttgatctggg aagcattccg tactcgctgg taatccagcc cttgcctgtt
     2161 ccttttataa aaggaggcac cttgtcgtct actgaagccg tgcaaataac tttggtatca
     2221 cccatctcta taagcactga gccttccgca tgctttaagt aattccttat tatttttaca
     2281 ggccttattt gttcgttgtt tctcccgcca attctctgca tctgtcttcc ttcccttcaa
     2341 aagtattgtt ttgtgtttag taatccctta cagagttgca taaattttct gccttgattc
     2401 aaaagttctg ttattatatt attccatgag cacggcagat ttatttttcc ctttcttccc
     2461 aagacatcca taaaaacata cagcttgtca gctttttttg cgatatgcag ggacattttg
     2521 aaacatcccc atatcgcttg ttttatgtaa ttcttattca taaaaattaa caaaaaccgg
     2581 aaccgatata tattcgtccg cacccacacc agcagtattt tcaacgatct tgccatttac
     2641 aaatatcttg gcttttgaaa tcgcaggaaa ttccttaagc gtaagcgcta ccgccttttt
     2701 cacagcattc tccgccgcct tgtctccctg attagccagt atgtgctcgg aaaaattcac
     2761 ataagctata ccgctgttta cctgcacatc cagcagttta gtaccctccg ggaaaggatt
     2821 tttcaggctg ccggcctccg ggccttccag cagtgcgctt aaggcggctt ccacactgtt
     2881 ttcaaagccg gataccactt ttgttaatgg aacaaaatat gaatatgccc cgcttccttt
     2941 tttatgatag tataccacaa cctttgataa cttttcacct gccttttcga caggctgaag
     3001 atttatttcc gttcttttaa gttcagaccc tacttcagtt ccctttggca tttcttcaat
     3061 ttttttgcca tccaccctga tttccactga ttttatgttt ggaaactctg taagcgtata
     3121 tgtcactgct ttgattccca gttcttcagc ctttttgctg ctgaagttca gaaattcctt
     3181 tgaaaaatct attatagcca aaccattttc ccggatgact gctccgttga ttttggtacc
     3241 cataggcagt gtaggtgtca atcctgcggc tttgagatcc tctaccgttt ccggactgta
     3301 aatgatagct gatattgctg tttttgctac gccaagatcc cccttgggca tatatcgcat
     3361 aacaggtata agaaagtctt cgttgtcttt gtagtaaagt accgttgctc tgctgtcttc
     3421 cggttgggaa acactgactt cactcatgct ttgggtttcc tcctgaactt gctgcggact
     3481 ttcctctacc gtatcagtct ttttgttaaa gaaatcccat aaagaaatca gcgggttact
     3541 gcagcttgta aggaccaatg tacagcatat aagtaatgcc aataatagtg caagattcct
     3601 gttcatttat ttcacccctt atttttttat caatatatta tattagcctc ataaggggta
     3661 tattcgtata agtggaataa tgcgacgggg acactgggac acggggacgt ttctcctgtc
     3721 ccgctttcag cgggacagga gaaacgtcc
//
LOCUS       DAOQUR010000111         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_30284_length_3527_cov_11.487327, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000111 DAOQUR010000000
VERSION     DAOQUR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30284_length_3527_cov_11.487327"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1213)
                     /gene="msrB"
                     /locus_tag="PKI50_12240"
     CDS             complement(266..1213)
                     /gene="msrB"
                     /locus_tag="PKI50_12240"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNT04502.1"
                     /translation="MNKEIWLAGGCFWGVEEYFSRIDGVVDTSVGYANGRTENPSYYD
                     IPHTGHAETVHVVYDADKTSLEVILEHFFGIIDPTILNRQGPDVGTQYRTGIYYSDEK
                     DREAVEQVIAKEQQKYKAPIVTEVKKLEHYYPAEEYHQDYLKKNPGGYCHIDFSSLKA
                     VSRPKVDPALYEKPSRDEIKKSLSDDQYSVTQENATELPFRNEYWDNHKAGIYVDIVT
                     GEPLFLSSDKYDSGCGWPSFTRPIDQAVLKEREDNSHFMHRTEVRSRVGNSHLGHVFD
                     DGPKDKGGLRYCINSASLRFIPVEKMEEEGYGDYIKYVK"
     gene            complement(1602..1769)
                     /locus_tag="PKI50_12245"
     CDS             complement(1602..1769)
                     /locus_tag="PKI50_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04503.1"
                     /translation="MEDKVFKLLTKMYSEMTERFKEVRIKLDEKADKSDIVRIENELN
                     PKSIPCLMDVS"
     gene            complement(1868..2803)
                     /locus_tag="PKI50_12250"
     CDS             complement(1868..2803)
                     /locus_tag="PKI50_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNT04504.1"
                     /translation="MIKENLLSEIEAAGFRVKVFNAEHLKEISSDFKILSEQGALDKE
                     FYTKSLTVFNYDYKSVLSNARSVMVIASPQRKSIVEFQCGDKTVMAVIPPTYMYSGIN
                     NQIISILNNVLAVEGYSFGKLVIPLKLIAVRSGLGRYGRNNVCYIPGLGSYIRLSAYV
                     TDYEFEEDSWGDIKVMESCSNCTACIDNCPTGAIDKGRFLIHAQNCITNFNEYGTPIP
                     EWISPLWHNAIVGCMKCQEACPHNAKLIELVDERICFDKKETGMILDESAINELPEET
                     RRKISHLDMEDYYDVLPRNIRLLYCCSTQCGRQEG"
     gene            2912..3448
                     /locus_tag="PKI50_12255"
     CDS             2912..3448
                     /locus_tag="PKI50_12255"
                     /inference="COORDINATES: protein motif:HMM:NF023134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNT04505.1"
                     /translation="MEFLCLEFVNSSWYITHKLFSDPLMNNEWLIKLFDKWNMQPLIE
                     PNELELAKLIDMRSRFMKLFTKIVEGKDLAKKDIELINSYMADISFFRQLQTKEGSLG
                     LYDIPKTRNWNWFMAEVSASFARLYSSEAINRLKICQNPECGWFFIDDSKSRNRKWCD
                     DTCASLMKVRRFRQRQKR"
BASE COUNT          980 a          805 c          610 g         1132 t
ORIGIN      
        1 cgtgtctcaa aacgtccccg tgtctcaaaa aaattgatgg ctctaattcc gctcaactaa
       61 gaaaaaccta acgctgtcaa aggtcgtccg tgaccgtgac agcttgctcg gcctccatgc
      121 ctcgcatacg gttgtttcta agtcgagcga gatttgagcc attaacaatt tctgtataag
      181 tatgctctga ctatacatta ctgttcctat tttgaacttt gtcagcaatt tgagcagcta
      241 attaagctgc cttgctttat ttgtgctatt tcacatattt tatatagtct ccgtaacctt
      301 cctcttccat tttttcaaca ggtataaatc taagtgaagc actgttgatg cagtacctta
      361 accccccttt gtccttgggg ccgtcatcaa aaacatgccc cagatggctg ttgcctaccc
      421 ggctcctcac ttccgtacgg tgcataaaat gactgttatc ctctctttcc ttaagcactg
      481 cttggtcaat agggcgggta aagctgggcc atccgcagcc tgaatcgtat ttgtcacttg
      541 aaagaaacaa aggctctcct gttacaatat caacataaat acctgcctta tggttatccc
      601 agtattcgtt tctgaaaggc aattccgtag cgttttcctg cgtaacactg tactggtcat
      661 cggataggga tttttttatt tcatcccggg agggcttctc atacaatgcc gggtccacct
      721 tcggccggga cacagccttc agggaagaaa agtctatatg gcagtatccc ccgggatttt
      781 tcttcagata atcctgatga tactcctccg cgggataata atgctccagc ttcttcacct
      841 cggtaacaat cggtgcctta tacttctgct gctctttggc aatcacctgc tctacagcct
      901 ctctgtcctt ttcatcggaa taataaattc ctgtgcggta ttgggtaccc acgtcagggc
      961 cctgcctgtt caaaattgtg ggatctataa taccgaaaaa atgctccagt ataacttcca
     1021 ggcttgtttt atccgcatca taaaccacat gtaccgtttc tgcatgccct gtgtgaggaa
     1081 tgtcataata agagggattc tccgtccttc cgttggcata tcccacagaa gtgtccacta
     1141 ctccgtctat acgggaaaag tattcctcca caccccaaaa acaccctccg gcaagccata
     1201 tttctttatt cataaaatca ccttaccttt tcaatacgtt ctaatatgtc agcaagcagc
     1261 ggggacggtt ctttctgctt ttgtacattt ttcgctttgt tattataata ccactttttc
     1321 gacagtctta gccaaactta actttttacc tgcaaacgaa acagcaggca gtgtaagtgc
     1381 tggatgcaaa atttcctgaa atctgattct ccttgatcat ttccttgata aaattctttt
     1441 gtactgttga tcccataaaa aatactcctt tctggctttt atctgattct tgcagaaagg
     1501 aggtaacttt ttcactcacc cacataatta tttacattac ctaccaatag accaatacaa
     1561 tctttttgat ggaatagcat ctacctgtaa tacttccttt ttcaacttac atccatcaaa
     1621 cagggtattg actttggatt caactcattc tctattctta ctatatcact tttatcagcc
     1681 ttttcgtcca gcttgattct tacttccttg aatcgttctg tcatctcaga atacattttt
     1741 gttaatagtt taaagacttt atcttccata tatatcatgc ccctttagaa tattataaca
     1801 tattttttag tgtagtcaaa aaatggtttt ttatcctata gtctattggg ttataccact
     1861 gtaatcttta accttcctgc cttccacatt gggtgctgca gcaataaagt agtcttatat
     1921 tcctgggcag aacgtcataa taatcctcca tatctaaatg agatattttc cttcttgttt
     1981 cttccggcag ctcattaata gcgctttcgt ccaatatcat ccctgtctct ttcttatcaa
     2041 aacatatcct ttcatccacc aattcaatca gctttgcatt atgagggcag gcttcctgac
     2101 atttcataca accgacaatt gcattatgcc ataggggact tatccactcc ggtatagggg
     2161 taccatactc gttgaaattt gttatgcaat tctgcgcatg tataagaaag cgtcccttat
     2221 caatcgcacc tgtagggcag ttatcaatac aagcagtgca attgctgcag ctttccatca
     2281 cctttatgtc accccagctg tcttcttcaa attcgtaatc agtaacataa gccgacagcc
     2341 ttatataact tcccagtcca ggtatgtagc aaacattatt tctgccgtac ctgcccaagc
     2401 cgcttctcac agcaataagc ttcagcggta tgacaagttt gccaaaactg tatccttcta
     2461 ctgcaaggac attattcagt atgcttataa tttggttatt gatgccagaa tacatgtaag
     2521 taggaggaat cactgccata acggttttgt caccacattg gaattcgaca atactcttac
     2581 gctgcggaga agcaattacc attactgatc ttgcattgct caatacactt ttgtaatcat
     2641 aattgaaaac tgtaagactc ttcgtgtaaa attctttatc cagagccccc tgctcggaaa
     2701 gtattttgaa atcactgctg atttccttca aatgctccgc attaaagacc ttaaccctga
     2761 agcccgccgc ttcaatttct gatagtaaat tttcttttat catattttcc cccttcttgt
     2821 aacccccaat ttgacagaat ccggttacac tattataata ataatatcat agggttatgt
     2881 aactgtcaat atatgaaatg gaggttacat catggaattt ttatgtttgg agtttgtaaa
     2941 cagcagctgg tatatcaccc ataaattatt ttcagatcca ttaatgaata atgaatggtt
     3001 aataaagctt tttgataaat ggaatatgca gccattgatt gagcccaacg agcttgagct
     3061 ggcaaaactt attgatatga gaagccgttt tatgaagctc ttcacaaaaa tagttgaagg
     3121 aaaagacctt gcaaaaaaag atattgagct aatcaacagt tatatggctg atataagctt
     3181 tttcagacag ttacagacaa aagaaggaag tttgggactg tatgatatcc cgaaaactcg
     3241 taattggaac tggtttatgg ctgaagtatc agcatccttt gcaagacttt actcctcaga
     3301 ggccataaat agattgaaaa tatgccagaa tcccgaatgc ggatggttct ttatagatga
     3361 cagtaaaagc agaaacagaa aatggtgtga tgacacttgt gcttctctta tgaaggtaag
     3421 gcgattcagg caaaggcaaa agcgataaat cggcacgccc tcgctctatt gcagcattgg
     3481 ctgcttaaat atcttgctga gaagtggaaa ccaaaaggcc tgatata
//
LOCUS       DAOQUR010000112         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_30830_length_3482_cov_10.473300, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000112 DAOQUR010000000
VERSION     DAOQUR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30830_length_3482_cov_10.473300"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /gene="rnpB"
                     /locus_tag="PKI50_12260"
     ncRNA           complement(<1..427)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI50_12260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(530..1240)
                     /locus_tag="PKI50_12265"
     CDS             complement(530..1240)
                     /locus_tag="PKI50_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNT04506.1"
                     /translation="MNLPLLYELQHLNKEMGAITRKLRELQEDKDMKKYKEEYQRLKE
                     EYLKGEEKLKKNAYRQEARNNEIRNIEYNMKSSLEIKFSKETNTVKKLENIEKQLEKL
                     EEKKQAAENEIIELINDADNINKELAEIKKKLAFIKKKFLNCKEDADKAAAGLKAQKS
                     ELQAKIDSMMKSIDQESLEMYNRLIKTHSDPVALVKNRICSGCKMEVPAMDYEALKGG
                     NQELKCQSCGRILFYKKS"
     gene            complement(1296..2417)
                     /locus_tag="PKI50_12270"
     CDS             complement(1296..2417)
                     /locus_tag="PKI50_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNT04507.1"
                     /translation="MGTIKCQTIAGMLDELAPRSLAESWDNVGLLVGDGRKRVSRIMV
                     CLDVTEWVIEEAVDNNVDMIISHHPMIFSGIKKVNTDTATGRKLISLVKNDISLYCLH
                     TNFDIVSGGLNDIFAKELGFSHWTIIEETYREKLFKLVVYVPAGQEDSVADAMCRAGA
                     GYIGKYSGCTFRSKGIGTFEPQEGAKPFLGSIGNLERVEEFRVETIVPEKLLSKVVGE
                     MLKAHPYEEAAYDIYETKNEGRVMGLGRIAELERETTLSMFAGNVKTLLNAENIRYAG
                     DPNRSIRKVALLNGSGSKFINSARLAGADVLITGDLQYHSTLEALEMGLCVIDAGHFS
                     TEKIMMKHITEFLKNKFRELKVEADVFESCSNTEILRVL"
     gene            complement(2405..3139)
                     /locus_tag="PKI50_12275"
     CDS             complement(2405..3139)
                     /locus_tag="PKI50_12275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT04508.1"
                     /translation="MQQQLYINNVRMMILNLTPRLKIIADSIQGFETLADIGSDHAYL
                     PIYLIKNRRVKSVIATDVNSGPAEISRKRIKNHGLESKISVREGSGLRVINSGEAEVI
                     VIAGMGGILIGDILDKGIDAAKSAKLLILQPMRDSEKVREWLLEHGFDIIDEELVKDQ
                     DKIYEVIWSQPGEEAGEAKGPLLIGEKIIEKKHPLLEEFINRKVKELEKIMIALEAMD
                     SDNCRERAEECRAMLAYYREVAEWAR"
     gene            3253..3444
                     /locus_tag="PKI50_12280"
     CDS             3253..3444
                     /locus_tag="PKI50_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04509.1"
                     /translation="MSKKLHIKPEVIQKFSWGGTTKEWEKEAEKLSNQLETMEAINNI
                     IEKQDCESEIKKPPGGGNK"
BASE COUNT          866 a          873 c          596 g         1147 t
ORIGIN      
        1 tgagtaagtc tataagccgg gttctgtatt ggatgatcat ctatctaggc ttatcgtcgc
       61 cgataagctc aagcgacctt acccggagac ggaacgggcc gttcctgcca aagcaaagcc
      121 ttggctgtct ctctatttgg tcttgctcca agtggggttt acatagccgg ttagttacct
      181 aaccgctggt gagctcttac ctcgcctttc cacccttacc gattgagaag tgagatgtga
      241 gatgtgggag gtgagactat aaatctgtcc cacttcccat ctctcacttc ccacctctca
      301 acggcggtat atctctgttg cactagcctt agagtcgcct ccaccgggtg ttacccggca
      361 ccctgcccta tggagcccgg actttcctcg tatgcataac tgcacacgcg atcatctggc
      421 ttactcaaaa tattatttta tgttttatcg tcagatattt attatatatc cgaaaggaga
      481 ttcagtcaat atgatatatt aagatgttat gccatatctt tacttagcat caggattttt
      541 tgtaaaataa aatccttccg cagctttggc atttcagctc ttgattgccg cctttcagcg
      601 cttcataatc catagcggga acttccatct tgcacccgct gcaaatgcgg tttttaacaa
      661 gggcaaccgg atcggaatgt gtttttatca gtctgttgta catttcaaga ctttcttgat
      721 caatggattt catcatgctg tcaattttgg cctgaagctc tgatttttgt gctttcaacc
      781 cggcagccgc cttgtcggca tcctctttgc aattcaaaaa tttcttttta ataaatgcca
      841 gtttcttttt tatctcagca agttctttat taatattatc cgcatcatta atgagttcta
      901 ttatttcatt ttctgccgcc tgcttttttt cttcaagctt ttcaagttgt ttctcaatgt
      961 tctccaattt tttgactgtg tttgtttctt tgctgaactt gatttctaac gatgatttca
     1021 tgttgtactc tatgtttcta atctcattat tccttgcttc ctgccggtaa gcatttttct
     1081 tcaatttttc ttcgccttta aggtattcct ctttgagcct ttgatactcc tccttatact
     1141 ttttcatgtc cttgtcttcc tgaagctctc tgagcttcct ggtgattgca cccatttcct
     1201 tattcagatg ctgcagttca taaagcaatg gcaaattcat cagaccatct cctttttcct
     1261 tttaaaaaaa taaaaatagg aatagctgac tattcctata atactctcaa aatttccgta
     1321 tttgagcatg attcaaaaac atccgcttca accttaagct ctctgaattt attctttaaa
     1381 aattcagtaa tatgcttcat cattatcttc tctgtgctga aatgcccggc atcaattaca
     1441 cacaagccca tttccagagc ttcaagagtg ctatgatact gcaggtctcc ggttatcagc
     1501 acatccgctc cggcaagcct tgcagaattt atgaatttgc ttcccgagcc gtttagaagc
     1561 gcaaccttcc tgatactcct gttcggatct cctgcatacc taatgttttc ggcattcagc
     1621 aaggttttca catttccggc aaacatggaa agagtcgttt ccctttccaa ctcggctatt
     1681 ctcccaagcc ccataacacg gccttcattt tttgtttcat atatatcata ggctgcttcc
     1741 tcataggggt gagcctttaa catctcccca accactttgc tcaaaagctt ctcgggaact
     1801 attgtctcca ccctgaattc ctcaactctc tcaaggtttc ctatgctgcc gagaaaaggt
     1861 tttgccccct cttgaggctc aaaggtgccg atccctttgc ttctgaaagt gcagccgctg
     1921 tatttgccta tataacccgc gcctgcacgg cacattgcat cggcaacact gtcctcttgc
     1981 cccgcaggta catagacaac caacttgaaa agcttttccc tgtatgtctc ttcaataatt
     2041 gtccaatgcg aaaatccgag ttcttttgca aaaatatcgt tcagcccccc tgacacgata
     2101 tcaaaattgg tatgaagaca gtacagggat atatcgttct taacaaggct aataagcttc
     2161 ctgcctgtgg cagtatccgt attaactttt tttattccgc tgaatatcat gggatgatgg
     2221 gatattatca tatcaacatt attatctacg gcttcttcta ttacccattc cgtaacatca
     2281 aggcaaacca tgatacggct gacgcgcttc cttccgtcgc ctacaagcag gcccacgtta
     2341 tcccagcttt cagcaaggct tctgggtgcc agttcatcca gcattcccgc aatagtctga
     2401 cattttatcg tgcccattca gccacctccc tgtaataagc cagcattgcc ctgcattctt
     2461 cggctctctc cctgcaatta tccgaatcca tagcttccag ggcaatcatt attttctcca
     2521 gttcttttac ttttctatta ataaattcct ccaatagagg atgctttttt tcaataatct
     2581 tttctcctat aagcaatggc ccctttgctt cccccgcttc ctctccgggc tgcgaccata
     2641 tgacttcgta tattttatcc tgatctttta ccagttcctc atcaattatg tcaaaaccat
     2701 gttcaagaag ccattccctt accttttccg aatctctcat cggctgaaga atcaaaagct
     2761 tggcactttt tgccgcatct atgcccttat ccaaaatatc tcctatgaga attcctccca
     2821 tgcccgcaat aacaatgacc tctgcttccc cagaatttat caccctgagg ccgcttcctt
     2881 cgcgcacgct tatcttcgat tccagtccat gattcttgat tcttttcctg gatatctctg
     2941 ccggaccgct gttcacatct gtggcaataa cgctttttac ccgtctgttc tttattaaat
     3001 atataggaag atatgcgtgg tccgaaccga tatccgccaa tgtctcaaag ccttgtatac
     3061 tgtctgctat aattttcagt ctgggagtaa gattcaatat catcatcctt acattattaa
     3121 tgtataattg ctgctgcatg cttcgacacc ctgctcagtt cccgtgtctt gccttattat
     3181 tatttattat actacacttt ataaattttt ttattaataa atgagaaaaa tacaataaag
     3241 gaagtgaaaa aaatgagtaa gaagcttcat atcaagccgg aggtaataca aaaattcagt
     3301 tggggaggaa ctaccaagga atgggagaaa gaagccgaga agctgtccaa ccagcttgaa
     3361 accatggaag caatcaataa tattattgag aagcaggatt gcgaaagcga aataaaaaag
     3421 cctcccggcg ggggaaacaa ataggtggat agtaaaaaac ctgatacttt cgtatcaggt
     3481 tt
//
LOCUS       DAOQUR010000113         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_33183_length_3304_cov_9.374885, whole genome shotgun sequence.
ACCESSION   DAOQUR010000113 DAOQUR010000000
VERSION     DAOQUR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33183_length_3304_cov_9.374885"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..986)
                     /locus_tag="PKI50_12285"
     CDS             complement(333..986)
                     /locus_tag="PKI50_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNT04510.1"
                     /translation="MERFSNISMAVVRRLPKYHRYLGDLLHHDIRRVSSQELSRITGF
                     TASQIRQDLNCFGGFGQQGYGYNVEELYNELGNILGLSRTYRTIIIGAGNVGRAIANY
                     TYFEESGFILYGMFDNSPKKIGDDVKGHKVLPIDEVEDFIKENEIEIAILCTPKEGTQ
                     KIADTLVNCGIKGIWNFAPVDLKLNSDVIIENVHLSESLFTISYLLKEREDLKKKKK"
     gene            1226..3142
                     /gene="abc-f"
                     /locus_tag="PKI50_12290"
     CDS             1226..3142
                     /gene="abc-f"
                     /locus_tag="PKI50_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HNT04511.1"
                     /translation="MIILSCKNINKSYGIDVILEDVTFTVQENEKVGIVGVNGSGKST
                     LLKIIAGDIEMESGEIFIGRNTSIGYMSQDFSFESNSTVYDELLTVFAPLIQMEKDLH
                     SLELRISGLGSSADKEIAESLLKSYADLQEEYKNSNGFGYKSQIKGVLKGLGFSEEDY
                     GKPVSLLSGGQKTRVALGKILLQNFNILLLDEPTNYLDIESVEWLEEYLKNLKCTALI
                     VSHDRYFLDMVTDRTFEIENKTLKEYDGNYSKFVERKQQLREMQLKDYAEQQKEIARQ
                     EAIIARFRQYNREKSIRQAESREKALSRIERIERPDAPVKNIGLKFEPSLRSGNDVLA
                     VENLEKSFDAPLFKNVSFEIKRGEKVALLGPNGIGKTTILKIIAGALKADSGHIRLGS
                     NVIAGFYDQEQESLNYNNTVIDEIWDEYPNMNQTELRSKLAAFLFQGEDVFKEISRLS
                     GGERSRVALLKLMLSKTNFLLMDEPTNHLDILSKEVLENALMNYSGTTLLVSHDRYFL
                     NKVATRIIELRQEGCLQYNGNYSYYLFKKKQTEAGTACREAEDSEAATVNKNDWLKQK
                     EEKSNLRRQQKRLEAVETEIEQCEKRIAEIDDLMKLPEVFTNHIKCQELHDEQEMLKK
                     KLDSLYEEWSELSV"
BASE COUNT         1012 a          676 c          712 g          904 t
ORIGIN      
        1 tttgccgatt ggcctgctat aaccctcgta ccgcctatca gcaatccatc gtctattata
       61 tatcctatgt ccctgtcttt tgcaactata aatgccttgt agcttttccc ggtaccgctc
      121 tgaccaacaa gcgcataaac ttccacggtc acacccccaa cacttttaga atggtttttc
      181 atataagtat tattctatca tatttagaag aatttgaaac aaaaaacccg caaatataat
      241 aatacacatt tgcgagcttc tgggcgttca atctgcaaat tcactaaacc tgcaccagat
      301 taaggcatat atcttgcata acctgtacat ttctactttt tcttcttctt aagatcctcc
      361 cgctctttga gcagatatga tattgtaaac aagctttcgc ttaagtgaac attctcaatt
      421 atgacgtcac tgtttagctt aaggtcgaca ggagcaaaat tccatatccc ctttatacca
      481 caattcacca gtgtatctgc tatcttctgc gttccttcct tgggtgtgca tagaatagct
      541 atctctattt cattttcttt aatgaagtct tctacctcat caatgggcag aactttgtga
      601 ccttttacat catcccctat ttttttgggg ctattgtcaa acataccata caaaatgaag
      661 ccgctttctt caaagtatgt gtaatttgct atagctcttc ctacattacc tgcgcctatt
      721 ataatagttc tgtatgttct gctcagtcca agtatatttc caagctcatt atacaattct
      781 tccacattgt agccatatcc ctgctgtccg aagcctccaa agcaattcaa gtcctgtctt
      841 atctgggaag cggtaaaacc tgttatcctg ctaagctcct gcgatgatac tctccgtatg
      901 tcatggtgca ataaatctcc tagatatctg tgatacttgg gaagacgtct gaccactgcc
      961 atggatatgt tgctgaatct ctccatacta tctaccccta acttttaaaa ttgaatcaac
     1021 tatatggtaa gaagttaata aatagattat attatattac ttactttatt gtttggtaaa
     1081 tttattttat tatatactga tattgttgtc aagaaattac agatattacc acattcttga
     1141 atgatattga actatgtaca aatcctgttc acttctgata gaataattat tgtgctatgg
     1201 atatatcacc ggcggaggtt attctatgat aattctttct tgtaaaaata taaataaaag
     1261 ctatggaata gacgttattc ttgaagatgt tacctttaca gtgcaagaga atgaaaaagt
     1321 cggcatcgta ggcgtaaatg gatcaggaaa gtcaaccctc ctcaagatta ttgccggcga
     1381 catcgaaatg gagtccgggg aaatattcat cggcaggaat accagcatcg gatacatgtc
     1441 ccaggatttc agctttgaat ccaatagcac agtatatgat gagctgctga ctgtttttgc
     1501 cccgcttatt caaatggaaa aggaccttca ctccctggaa ctcagaatca gcggtctggg
     1561 aagctccgct gacaaggaaa ttgcagagtc cctgcttaag tcctatgcag atttgcagga
     1621 agaatacaaa aacagcaacg gttttggcta taaaagccag ataaagggtg tgcttaaagg
     1681 gctgggcttt tcggaggagg attacggcaa gcctgtttcc ttactcagcg gagggcagaa
     1741 aacaagggtt gccctgggta agatactact gcagaatttt aatatactgc ttttggacga
     1801 accgaccaat tatcttgata tagagtctgt ggagtggctg gaggaatacc ttaagaacct
     1861 taaatgtacg gcgcttatag tatcccacga ccgctacttc ctggacatgg tgactgacag
     1921 aacctttgaa atcgaaaaca aaacccttaa agaatatgac ggcaattatt ccaaattcgt
     1981 tgagagaaag cagcagctca gagagatgca gttaaaggat tatgcggagc agcaaaagga
     2041 aatcgcccgt caggaagcaa taatagccag attcaggcaa tataacaggg agaagagcat
     2101 aaggcaggcg gaaagcaggg aaaaagccct cagccgtatt gaaaggattg agaggcccga
     2161 tgcccctgta aagaatatag gactcaaatt tgaaccgtct cttagaagcg gtaatgatgt
     2221 ccttgctgtg gaaaacctgg agaaatcctt tgatgcaccg ctcttcaaga atgtgagctt
     2281 tgaaattaaa cggggcgaaa aggttgcgct gctgggccct aacggaattg gcaaaactac
     2341 catccttaag ataatagcag gggcattaaa ggcggattcc ggccatataa gacttgggtc
     2401 caatgttatt gcaggcttct atgaccagga gcaggaaagc ctgaattaca ataatacagt
     2461 aattgatgag atatgggatg aataccctaa tatgaaccag acagaactgc gttcaaaact
     2521 ggctgcattt ctgttccagg gagaggatgt attcaaggaa atcagcagac tcagcggcgg
     2581 tgaaagaagc agggtcgccc ttttgaagct tatgctgtcc aaaaccaatt tcctgcttat
     2641 ggatgagcct accaaccatt tggacatttt atcaaaggaa gtactggaaa atgcactgat
     2701 gaactactca gggacaacgc tgcttgtttc ccatgacagg tattttctga acaaggtcgc
     2761 caccaggata attgaattga ggcaggaggg ctgcttgcag tacaatggaa actactccta
     2821 ttacttattt aagaagaagc aaacggaagc cggcacagct tgccgggagg cggaggattc
     2881 cgaggcagcc actgttaata agaatgattg gctgaagcaa aaggaagaaa aatcaaatct
     2941 gcgaagacag cagaagaggc ttgaagctgt tgaaacagaa attgagcaat gcgaaaaacg
     3001 tatagctgaa atcgatgatt tgatgaagct tccggaagta ttcacaaatc atataaaatg
     3061 ccaggagctt catgatgaac aggagatgct taagaagaag cttgattccc tgtatgaaga
     3121 atggagcgag cttagcgtat gatagcaaag gtttgtaaag tactccgact gtaggggaag
     3181 tatttcctcc ttaccttcca aagcggggga tgcaaggggg cgaagcaccc tgccgataaa
     3241 ggagggtgct caggatgcgg agcatccgtc gaagggaacc atagggttcc ctagcgaggg
     3301 aaaa
//
LOCUS       DAOQUR010000114         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_33340_length_3293_cov_9.813774, whole genome shotgun sequence.
ACCESSION   DAOQUR010000114 DAOQUR010000000
VERSION     DAOQUR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33340_length_3293_cov_9.813774"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..727)
                     /locus_tag="PKI50_12295"
     CDS             complement(11..727)
                     /locus_tag="PKI50_12295"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNT04512.1"
                     /translation="MSRNIRVFFAGFLCCILLTAGMMAVIGEPADTEVKAILSNTIKM
                     KLFGNDFAPKNDDGTYLKPLIYNGRTYLPVKPLGDALGIAVEWDGVNKIVWLGGKTQI
                     VPINDTSMYEDYSRTIVTTDTDKLTTADTTYKWGIANISPLNSVYYGCCLKPEGKYKR
                     FTASIFLSEDTKKELTMDIRKDNKQGVVLKSLQLKPGETVEIDIDISGVDTLFIFSKV
                     GSTQKLIIGEPAFRNDTTPQ"
     gene            complement(860..1696)
                     /locus_tag="PKI50_12300"
     CDS             complement(860..1696)
                     /locus_tag="PKI50_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HNT04513.1"
                     /translation="MAEALVNKILPFSSVDGPGNRTVIFFQGCNFNCLYCHNPETINR
                     CTGCGLCLDVCPAGALRSRNGRIEWNSANCTGCDACLAACPGKSSPKAVSMSVNDIMK
                     TVEKVKPFISGITVSGGECTMQKEFLAELFDAVHKLGLTSYVDTNGCIDFSKEPELTF
                     AMDKAMLDIKSYDDGEHRMLTGMSNALVLKNAEFLANKQKLYEIRTVIVPGILDNNRN
                     VAEVSKLIAKLDADIRYKLIRFRPVGVAGDIKKVGIPSDELMEELKKTAEKNGCRYVV
                     VV"
     gene            complement(1689..3182)
                     /locus_tag="PKI50_12305"
     CDS             complement(1689..3182)
                     /locus_tag="PKI50_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HNT04514.1"
                     /translation="MSEVLSIIKDRTLTYEQKIVSLARAAENTLSVLPISKEIQEYRR
                     EGIICDLNEGNAPYRPRYIVPDYERFMRQGSEFLRLKPARDIWEATNNLLILYSHVPS
                     VTTMPVYVGNLDTLLEPFIKDEEEAYKAVRLFLMHLDRTVTDSFCHANIGPGATKAGR
                     LILRAEKELQDAVPNLTIKYSKDTPEEFALEAVETALVSAKPSFANHEMFVNDFGINY
                     SIASCYNGLPIGGGSYTLVRLVLARLAKQSNNAGHFLEEVLPEAVSKMREFMDQRIRF
                     IVEESGFFESSFLADESLIYKDRFTAMFGMVGLAECVNHLLGAKEQKDRFGHNDAANA
                     LGQRIIERMHQLVSEHNNQYCKATDGRFLLHAQVGIDTDYGISPGCRIPIGEEPEIYE
                     HLLQSAPFHKYFPSGIGDVFSFDETVKRNPAYLLDIIKGGFSEGLRYISFYASDSDLI
                     RITGYLVKKSDMKALQQGKQALHDTVALGLGAVQNSGILERKVRHNG"
BASE COUNT          827 a          824 c          617 g         1025 t
ORIGIN      
        1 acgccgcatt ttattgcggt gttgtatcat tccgaaaagc cggctctcct attataagct
       61 tctgggttga acccaccttt gagaatatga aaagagtatc taccccactg atgtcaatat
      121 ctatctcaac agtctccccg ggctttagct ggaggctctt cagaaccaca ccctgcttgt
      181 tgtctttcct gatatccatt gtcaattctt ttttggtatc ttcactcagg aatatcgatg
      241 cggtaaagcg cttgtatttt ccttcaggct tcaagcagca gccgtagtat acgctgttta
      301 aaggactaat gtttgcaatt ccccatttgt acgtcgtatc tgcggttgta agcttatctg
      361 tatcggtagt cactattgtc cgtgagtaat cctcatacat gctggtatca ttaatgggaa
      421 cgatctgtgt tttcccgccc agccatacga ttttgttgac tccatcccat tcaaccgcta
      481 ttccgagagc gtctcccagg ggcttcaccg gcagatatgt cctgccgtta tatataaggg
      541 gcttcagata agttccgtca tcgtttttgg gagcgaaatc attgccaaaa agcttcattt
      601 taatggtatt tgacaatatt gcctttactt ccgtgtctgc aggctctccg attactgcca
      661 tcatacctgc tgtcagaaga atacagcaca gaaaacctgc aaaaaatacc ctgatattcc
      721 tagacataac aacacacctt tctgtttaaa tttcatagtg tcctacatta tatatgatag
      781 ataaatgaaa ggtatgccgc tttattattg ttgctgtact ttttattcaa attgctgact
      841 tgtctttaaa tgttcaattt tacaccacta caacatatct gcagccgttt ttctcagctg
      901 tttttttaag ctcctccatc aactcatctg atgggatacc gacttttttg atatcgccgg
      961 caactcctac cggcctgaac ctaatcagct tgtaccgtat atccgcatcc aattttgcaa
     1021 tcagcttgct cacttcagca acattacggt tattgtcaag aatccccgga actataacag
     1081 tccttatttc atatagcttt tgcttgtttg ccaggaattc cgcattctta agcactaatg
     1141 cattggacat tccggtaagc attctgtgct ctccatcgtc ataggacttt atatcaagca
     1201 ttgccttatc catagcaaat gtcagctccg gttcctttga aaaatcaata cagccgtttg
     1261 tatcaacgta ggacgtaagc cccaatttgt gcaccgcgtc aaaaagctcc gcaaggaatt
     1321 ccttttgcat ggtgcactcg cctcccgata cagttattcc tgatataaaa ggcttcactt
     1381 tctcaaccgt tttcattatg tcatttactg acattgaaac agcctttgga ctgcttttgc
     1441 cggggcatgc cgcaaggcag gcatcacaac ctgtacagtt tgcggaattc cattctattc
     1501 ttccgtttct gcttcttaac gcacctgcgg ggcatacgtc caagcacagc ccgcagccgg
     1561 tacatctgtt tattgtctca ggattatggc agtacaggca gttgaaattg cagccctgaa
     1621 aaaatatcac cgtacgattt ccggggccat ccacagagct gaagggcagt attttattca
     1681 caagtgcttc agccattgtg ccttaccttt ctttccaata ttccggaatt ctgtactgcg
     1741 ccaagcccca atgcaacagt gtcatgcagc gcctgcttcc cttgctgaag cgccttcata
     1801 tccgacttct tgacaagata tccggttatt ctgattaaat cactgtctga agcatagaag
     1861 gatatatacc tcaggccttc cgaaaaaccg cccttaataa tatcaagcaa ataggcggga
     1921 tttctcttta ccgtctcgtc aaagctgaac acatctccaa tccctgatgg aaaatattta
     1981 tgaaagggag cgctctgcag cagatgttca tatatttccg gctcctcccc aatgggtatc
     2041 cggcatcccg ggctgatgcc gtaatcggta tcaatcccaa cctgggcatg gagcagaaaa
     2101 cggccgtcgg ttgctttgca gtactgattg ttatgctccg ataccaattg gtgcatcctt
     2161 tctattattc tttgcccaag ggcatttgcg gcatcattat gcccgaacct gtccttttgt
     2221 tcctttgccc caagcaagtg attcacgcat tccgcaagcc cgaccatacc aaacatggct
     2281 gtaaacctgt ctttatatat taagctctca tccgcaagga agctgctttc aaagaaaccg
     2341 ctttcttcaa caataaacct gatgcgctgg tccataaact cgcgcatttt agaaaccgct
     2401 tcaggaagta cttcttcaag gaagtgccca gcgttgtttg attgttttgc caatcttgca
     2461 agtaccagcc ttacaagagt atagcttcct ccccctattg gcagcccgtt ataacagctg
     2521 gctattgaat aattgatacc aaagtcatta acaaacatct catgatttgc aaagctgggt
     2581 tttgcagata caagggctgt ttccaccgct tccaatgcaa attcttcagg agtatcctta
     2641 ctgtatttga ttgtcaggtt cgggacagca tcctgaagct ctttctcagc ccttaaaatg
     2701 agtcttcctg cttttgttgc ccccgggcca atgttcgcgt ggcagaatga atcggtaacg
     2761 gtacggtcca ggtgcatcag gaataacctg acagccttat atgcctcctc ctcgtcctta
     2821 ataaaaggct ccagcaaagt atccaggttc cctacataca caggcattgt agtaacggag
     2881 ggaacatgac tgtacaatat caacagattg ttcgtggctt cccatatatc ccttgcaggc
     2941 ttcagtctca ggaactcact gccttgtctc ataaaccttt catagtcagg tactatgtaa
     3001 cggggcctgt acggcgcgtt tccctcattc aaatcgcata ttattccttc tcttctgtat
     3061 tcctgtattt cttttgatat cggaagaaca ctcagggtgt tttctgcagc ccgggccaag
     3121 cttactatct tttgttcata agtcagtgtc ctgtccttga ttatacttaa tacttcactc
     3181 attttaacac cttcaataaa aattacatgt tagtaattgt tttacctata ttataccatt
     3241 tattattttg atatgctata aagtaggctg ttgtacaaaa tatgaagaat gcg
//
LOCUS       DAOQUR010000115         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_34036_length_3250_cov_12.121127, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000115 DAOQUR010000000
VERSION     DAOQUR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34036_length_3250_cov_12.121127"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    76..325
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            398..1561
                     /locus_tag="PKI50_12310"
     CDS             398..1561
                     /locus_tag="PKI50_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNT04515.1"
                     /translation="MTTKKSFDEINEKIRKGKAVVVTAEELTGIVEEKGISDATKYVD
                     VVTTATFGPMCSTGAFLNFGHSDPPIRMEKIELDNVSAYGGLAAVDAYIGAAEPSTDR
                     GIEYGGAHVICNLIDGKKVHLKAYSKGTDCYPRKEIDTYITKDSINEAYLYNPRNCYQ
                     NYSAAANTSDRTLYTYMGVLQPGLGNVNYSTSGELSPLLKDPYLRTIGIGTRIFLAGA
                     EGYVAWQGTQFASGKPRGENGIPLSGACTLAVIGNLKEMSTEYIQPAIFEKYGVSMFV
                     GIGIPIPVLDSDLLFNLSVQNKDIYTNILDYSVQRSNKPVLAQCSYEELRSGFVSIEG
                     KKVKTSPLTSLYKSREIAQVLKSWVAEKGFTLQPPVAPFPKGVTLKSLEQGRL"
     gene            1563..1781
                     /locus_tag="PKI50_12315"
     CDS             1563..1781
                     /locus_tag="PKI50_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT04516.1"
                     /translation="MQGCIHCGACTAACTKGALKMEPESGIVSCDESLCTHCNACIRA
                     CPARALTYHQIRRPYVSEKRLPEAYESR"
     gene            1738..2460
                     /locus_tag="PKI50_12320"
     CDS             1738..2460
                     /locus_tag="PKI50_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNT04517.1"
                     /translation="MYQKRDYRKLMKADDLICFNVTEYESDLLIYARTNLKKEAKIAV
                     AKYRKDIIDYIRRHPEFLTSLVPINPILDAPEIIQDMCHCSKLANVGPMAAVAGAVSK
                     YAGRDLLRFADEVLIENGGDLFIKSKNDRRILIYAGNSPLSNKVALLIPGNNQELGIC
                     TSSGTVGHSLSFGKADAAVVISKDAVLADAAATAVGNVVKDKNHIEAGIEYAMSIPGV
                     EGVVIIADDKMGACGDINITKP"
     gene            2475..>3250
                     /locus_tag="PKI50_12325"
     CDS             2475..>3250
                     /locus_tag="PKI50_12325"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNT04518.1"
                     /translation="MKIKEFFKNKPVISLEIFPPKPEMPIDTIFNTIDALSGLKPAFM
                     SVTYGAGGSSKAHTVKIADVIKNKYNIEALAHLTCIGAKRDEIDNILKQLKECHIENI
                     MALRGDIPVGSDPASLENAEYKYAKDLISYIKEKDSFCIGAACYPEGHIECPDEVNGL
                     KHLKLKADSGADFLITQLFFDNNLYFSLLEKLDIFDINLPVSAGIMPVINKRQIERIT
                     ALCGASIPPKIKRILDKYGDNPEALKEAGIAYATEQIIDLL"
BASE COUNT         1039 a          641 c          755 g          815 t
ORIGIN      
        1 tctcaaaagt acaaaattta tatcttgaca ttgctgaaat aatggggtat attatatttt
       61 aaatatttaa gaaccataat ggtgatgatg ggaagagtaa gttggagcga gtggcaaaga
      121 gagccgggaa gggtggaaac cggtacatga taccagccga acatggtctc tgaactgtat
      181 aggtgaggca gttgcttagc ttataccggc aggctgacgt tatacagcac ggtgataaat
      241 catttttgag gagtcacttg gtaagttttt tgtcgtgagg caaaaaagaa tcagggtggt
      301 aacacgaata gctttcgtcc ctttattggg gcggaagctt tttaatttac tataccttgt
      361 cttaccgcaa caaattatat caggaggtat agcaaaaatg acaacaaaaa agagctttga
      421 tgaaattaac gagaaaatca ggaaaggtaa agcagtagta gtaacggccg aggaattaac
      481 aggtatagta gaggaaaagg gtatttccga cgcaacaaaa tatgtagatg tggtaacaac
      541 agcaaccttt ggtccaatgt gttccaccgg tgcctttctt aacttcgggc attccgatcc
      601 tccgatcaga atggaaaaaa ttgagctgga caatgtaagt gcatacggag gacttgcagc
      661 ggtagatgca tatataggag cagcagagcc ctcgacggac agaggtatag aatacggcgg
      721 cgctcatgta atatgcaatt taatagacgg caaaaaagtg catcttaagg catactcaaa
      781 gggcacagac tgttatccga gaaaagaaat agatacttat atcaccaagg attcgataaa
      841 cgaagcctat ttgtacaatc cgagaaattg ctatcaaaac tactccgcag cggcaaatac
      901 ctctgacagg acactgtaca catacatggg agtacttcag cccggcttgg gaaatgtaaa
      961 ttattctact tcaggggaac tcagcccgct gttgaaagac ccctacctca ggactatagg
     1021 tataggaaca agaatctttt tggccggtgc agaaggttac gtagcctggc agggaaccca
     1081 atttgccagc ggaaaaccca gaggtgagaa tggaatcccc ctgtccggcg cctgtaccct
     1141 tgcggtaata ggcaacctta aagagatgag cactgaatat attcagccgg ctatttttga
     1201 aaaatacggt gtttcaatgt ttgtcggaat aggaatacct atacctgtcc ttgattcgga
     1261 tctgcttttc aatctttcgg tacaaaacaa agacatatat acaaatattt tggattacag
     1321 cgtacaaaga agcaataaac cggtcttggc acaatgcagc tatgaggaat tgagaagcgg
     1381 atttgtttcc attgaaggta agaaagtaaa aacttcacct cttacaagcc tgtataaatc
     1441 aagggaaata gcccaggtac taaagagctg ggttgcggaa aagggcttta cattacagcc
     1501 tcctgtggcc cctttcccta agggagtaac gctgaagtca ctggaacaag ggaggttata
     1561 gtatgcaagg atgcattcat tgcggcgctt gtaccgcggc ttgcaccaaa ggtgctttga
     1621 aaatggaacc cgaatcggga attgtcagtt gtgatgaatc cctttgcaca cactgcaacg
     1681 cttgtataag agcatgcccg gcaagagcac ttacctatca tcagataagg aggccttatg
     1741 tatcagaaaa gagattaccg gaagcttatg aaagcagatg atctgatatg cttcaatgta
     1801 actgaatatg aatccgattt gctgatatat gccaggacaa acctaaagaa agaagcaaaa
     1861 attgctgttg caaaatacag aaaggatatt attgattaca tcagaagaca tccagagttt
     1921 ttgacaagcc tggtgccgat caatcccata ttggacgccc ctgaaataat acaggatatg
     1981 tgccattgct caaagcttgc caatgtaggc ccaatggcag cagtggcagg ggctgtttca
     2041 aaatatgcag gcagagatct gctccgattt gcagatgaag tactgataga aaacggaggt
     2101 gatttattca ttaaatcaaa gaatgacaga cggatactta tatatgcggg caattccccc
     2161 ctttcaaaca aggttgccct ccttattccc ggcaataatc aggaactggg gatatgcacc
     2221 tcaagcggaa ctgtcggcca ttctttaagt tttggcaaag cagatgcagc cgttgtgatt
     2281 tcaaaggatg ctgttttagc tgatgccgca gccaccgctg tcggaaatgt ggtaaaggat
     2341 aaaaaccata ttgaggccgg aatagaatat gccatgtcta ttccgggtgt agagggagta
     2401 gtaatcattg cagatgacaa aatgggtgcc tgcggtgata ttaatattac aaagccttga
     2461 gaggagaatt ggttatgaaa ataaaagagt tttttaagaa caagcctgtt atatccctgg
     2521 aaatatttcc gccgaaacct gaaatgccaa tagatacaat atttaatacc attgatgctc
     2581 tgtccggtct taagcctgca tttatgagcg taacttacgg tgccggcggc agcagcaagg
     2641 ctcataccgt taaaattgcc gatgtcatca agaataagta taacatagaa gcccttgcac
     2701 accttacctg cataggcgca aaacgggatg aaattgacaa tatacttaag cagcttaaag
     2761 aatgccatat tgaaaacata atggctttga ggggagacat acctgtgggt tcggatcctg
     2821 cttccttaga gaatgccgaa tacaagtacg ccaaagatct aatatcctat ataaaagaaa
     2881 aggacagttt ctgcatcggc gctgcctgct atcccgaagg ccatatcgag tgccccgatg
     2941 aagtaaatgg tttgaagcat ttaaagctaa aagcggattc cggcgccgac tttctgatta
     3001 cccagctttt ctttgataat aatctatact tctccttatt ggaaaagctt gatatattcg
     3061 atatcaacct tccggtctcg gccggcatta tgcctgtaat aaacaagagg cagattgaac
     3121 gcatcacagc tctttgcggt gcctctattc ccccaaagat taaacgtata ctggacaaat
     3181 acggagataa ccccgaagca ttgaaggaag cgggtatagc ctacgcaaca gagcagataa
     3241 tagacctgct
//
LOCUS       DAOQUR010000116         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_34113_length_3245_cov_9.678370, whole genome shotgun sequence.
ACCESSION   DAOQUR010000116 DAOQUR010000000
VERSION     DAOQUR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34113_length_3245_cov_9.678370"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="PKI50_12330"
     CDS             complement(<1..1538)
                     /locus_tag="PKI50_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNT04519.1"
                     /translation="MRPRYLELEGLQSFKELQSIDFDRLGETGLFGIFGPTGSGKSTI
                     LDAITLALYGNVQRANRGTQGILNLSSANVRVSFTFDLVKDKKRKSYKVEREYRRKKD
                     SENSIESRTARLIELNPSGDAVIADKPGEVNEAVIELIGLQFDDFTRSVVLPQNKFQE
                     FLLSAKGDKTKMLERIFYLEEYGRELTEKVNRRLGRIRNRLSGVERALSVLGDISDAS
                     LKDSEAGLIAAEKHRKSVAEALRAAEDEYYGAKEVWELSAEYRETEEKYQELLAGRAE
                     IDLMRISCKGAEAARSLMDRINDIKKTGGELENVTKELERLNTSLCELEGQLEQGQAE
                     LESATKARQEKIPLLIGHRTKLEKVLKTKSELEKIEEVLRKLRNEHVILKKKVDDWNE
                     TIAKQKSGLEGLLKEAAGKKARTEAIKVEAGCRDKIRYGASLEEDLERLQKEKEKQKA
                     KYEEMADIIIRQEQEIESFKETGLKLLKEKEAIQSSYEQHKKSKKWDRNDIHKEETKY
                     YSIRS"
     gene            complement(1519..2775)
                     /locus_tag="PKI50_12335"
     CDS             complement(1519..2775)
                     /locus_tag="PKI50_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HNT04520.1"
                     /translation="MRILHTSDWHLGKTLDNINRLDEQRQFIDELCGIAESESADLVL
                     IAGDIFDAYNPSSAAEELFYDAVDRLNNKGRRAVVVIAGNHDSPDRLCAASPLAYKNG
                     IVLLGYPGSDAGVFGSSSILQSGPGWLEIMPGASEHSAVIITLPYPSESRLEQLISVD
                     SSEEKLQEAYSDKISELLHNLSGNFRNDTVNIIVSHLYLRDGRTSDSERQLGGALVVD
                     PCRLPDNAHFIALGHLHRPQRIKSAPSPTYYSGSPLAYSFSETDYSKLVYIVDAVPGR
                     ESEVKEVYLNSGKPLKQWKAKNGIMEALRWCEEGRDMNAWIDLEVYTDRALTLEEQKL
                     LRELNPGIINIRPVVTDEKESIIDFESREGKKIEELFRDYYRYKTGMEAAEEMMESFL
                     EVINDNQEDDLEEIQPGGEESEAQVS"
     gene            complement(2965..>3245)
                     /locus_tag="PKI50_12340"
     CDS             complement(2965..>3245)
                     /locus_tag="PKI50_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04521.1"
                     /translation="GKKNKDDSNGDKKNNSQVRDKDKDNSSSNNDSQKKTNNSSSSND
                     SQEKVNNSKNSKSKIDNNGKINWNNWIYNFDWLNKNNDSDKNSRSRER"
BASE COUNT          690 a          900 c          576 g         1079 t
ORIGIN      
        1 gacctgatac tatagtattt tgtctcttct ttatgtatgt cattcctgtc ccactttttg
       61 cttttcttgt gctgttcata ggagctttga attgcttctt tctctttgag aagcttcaat
      121 cctgtttctt taaaggattc aatctcctgc tcctgccgga ttataatgtc tgccatctct
      181 tcatatttgg ctttctgctt ctccttttcc ttctgcagcc gctccaaatc ttcttcgagg
      241 cttgccccat accggatttt atccctgcag cccgcttcaa ccttaatggc ttcagtgcgg
      301 gctttctttc cggctgcttc cttcaggagc ccttcaagcc cggacttctg ttttgctatg
      361 gtttcgttcc aatcatcaac ttttttcttc agaattacat gttcattcct aagctttcta
      421 agaacttcct ctattttttc cagttcactt tttgtcttaa ggaccttttc aagcttagtc
      481 ctatgcccta tgagaagggg tatcttctcc tgtcttgcct tagttgcaga ttcaagctct
      541 gcctgtcctt gctcaagctg tccttccaat tcgcaaaggc ttgtatttag cctctccagc
      601 tcttttgtca cattctccaa ttccccgccg gttttcttaa tatcattgat cctatccata
      661 aggctccgtg ctgcttctgc tcccttgcag ctgatcctca ttaagtctat ttcagccctg
      721 cctgcaagca gttcctgata tttttcctct gtctcacgat attccgcaga aagctcccag
      781 acctctttcg caccgtaata ttcatcttcc gccgccctca aggcttcagc cacgctcttc
      841 ctatgctttt ccgccgcaat taaaccggct tccgaatcct ttaatgaagc atctgaaata
      901 tcccccaata cggatagtgc cctctcaact cccgaaagcc tgttacggat tcttcccagc
      961 cttctgttga ccttttccgt aagttcccgt ccgtactctt caaggtagaa tatgcgttcc
     1021 agcatttttg tcttgtcacc cttggcagat aaaaggaatt cctgaaattt attctgggga
     1081 agcactaccg aacgtgtaaa atcgtcaaac tgcagcccta tgagttctat tacagcctca
     1141 ttcacttctc ccggcttgtc ggcaataacc gcatcccccg agggattcag ctctatcagc
     1201 ctggctgttc ttgattctat ggagttctca gagtccttct tccgcctgta ctccctttcc
     1261 accttgtatg actttctctt cttgtctttc accagatcaa aggtaaatga aacccgcaca
     1321 ttggctgaag acaagtttaa tataccctgg gtgcctctgt ttgctctctg gacatttccg
     1381 tacaaagcca aggttatagc atccagtatg gttgactttc cgctgcctgt agggccgaat
     1441 atcccaaaaa ggcctgtctc cccaagcctg tcaaaatcta ttgactgaag ctccttaaag
     1501 ctttgcagcc cctctaattc aagatacctg ggcctcactt tcctcacccc ccggctgtat
     1561 ttcttccaag tcatcttcct gattatcatt tataacttca aggaaggatt ccatcatttc
     1621 ttcggcggct tccattcctg tcttgtatct gtaataatcc ctgaacagct cctctatctt
     1681 tttcccttcc ctgctttcaa aatctattat gctttccttc tcatcggtta ccaccggcct
     1741 tatgtttata atcccggggt taagctccct aagaagcttt tgttcctcca aggtcagagc
     1801 cctgtcggta tatacctcca agtctatcca ggcgttcatg tcccttcctt cctcgcacca
     1861 tctcaacgct tccattattc cgttcttagc cttccactgc ttcaacggct ttccggaatt
     1921 caggtaaacc tcctttacct cagactctct tccgggtact gcatccacta tatataccaa
     1981 tttgctgtag tccgtctccg aaaaactgta tgcaagaggt gaaccggagt aatatgtcgg
     2041 tgaaggggcg ctttttatcc tctgaggcct gtgaaggtgc cccaaagcaa taaaatgagc
     2101 attatcagga agccggcaag gatctacaac caaagcgcct ccaagctgcc tttcggagtc
     2161 tgaagtcctt ccgtccctaa gatacaaatg acttactatg atgttcactg tatcattcct
     2221 gaagttgccg ctcaaattat gcaaaagctc tgatatcttg tcggaataag cctcctgaag
     2281 cttttcttca ctggaatcaa ccgatatgag ctgctcaagc ctggattccg aaggataggg
     2341 cagggtgatt atcacagcgc tgtgctcgga agcaccgggc attatctcaa gccatccggg
     2401 gcctgactgc agtatggaac tacttccaaa cacccctgca tcgcttccag gatatccaag
     2461 cagtacaata ccgttcttat atgccaaagg gctggcggcg cacagccggt cagggctgtc
     2521 atggtttccc gcaattacga caacagcccg cctgccctta ttgttcagcc tgtcaaccgc
     2581 atcgtaaaac aattcctcag cggcggaaga cggattatat gcatcaaaaa tatccccggc
     2641 tataagcacc aggtctgccg attcactttc tgctattccg caaagctcgt ctatgaactg
     2701 cctttgctcg tctaatctgt taatattgtc gagagtcttg cctaagtgcc agtctgaagt
     2761 atgaagtatg cgcacggcca ttcccctcct tgtgcttgac ttattccatt atctttattc
     2821 tatcgaatta tttgccaaaa tacaatttaa tatgtggtta acataacaat aggacacggg
     2881 aaggacacgg ggacgtttct cctgtctccc tgaaagggag acaggagaaa cgtccccgtg
     2941 tccttcccgt gtcctcgttg ctttttatct ttctctgctt cttgaatttt tatcgctgtc
     3001 gttgttttta tttaaccaat cgaaattgta aatccagtta ttccaattaa tcttaccgtt
     3061 gttgtcaatc ttgcttttac tgttcttgct gttatttacc ttctcttgag aatcattgct
     3121 actgctgctg ttattcgttt tcttttgaga atcattgtta ctgctgctgt tatctttatc
     3181 tttgtctctc acctggctat tattcttctt atctccgtta ctatcatcct tattcttttt
     3241 tccgc
//
LOCUS       DAOQUR010000117         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_35872_length_3139_cov_11.631647, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000117 DAOQUR010000000
VERSION     DAOQUR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35872_length_3139_cov_11.631647"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKI50_12345"
     CDS             <1..467
                     /locus_tag="PKI50_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNT04522.1"
                     /translation="IIQKCTEMGIKRIVPVLTSRTVVRLESEKDERKKAERWAKIAEE
                     AAKQSGRGIIPVIGMPVTLDQALRSAAGLDLVIIPYELETSLPVKEVLSKNKAGSIGF
                     FIGPEGGFEPVEVEKAKEMGAVPVTLGRRILRTETAGIAVLTSIMYEYDQMK"
     gene            479..1780
                     /gene="mtaB"
                     /locus_tag="PKI50_12350"
     CDS             479..1780
                     /gene="mtaB"
                     /locus_tag="PKI50_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNT04523.1"
                     /translation="MKKAAFYTLGCKVNQYETEAMTESFENAGYEIVDYSEFADVYII
                     NTCTVTNMGDRKSRQIIRRAREKNPEALVAVVGCYSQIAPGEVLEIPEVSLVVGTDER
                     SRMVELVEYAMEKEEKLNMVNDIMKVKEFEEMSIKSYKSRTRAFLKIQEGCDQYCTYC
                     IIPYARGHIRSRKPDSIIAEVKELAENGFREIVLTGIHVASYGKDLGNTSLIDIIEKV
                     HEIDGIRRIRMSSVDPNVMTDGFIERLSRLPKICGHFHLSLQSGCDETLKRMNRKYTT
                     GEYRRVVEKLREVFADVAITTDLIVGFPGETEEEFQKTVDFVEEIAFSAMHVFKYSQR
                     SGTPAAKYENQIKPQVKDSRSKVITAIAQKNEEKFKKAFIGRSKPVLYEQPFDGQKSL
                     FEGLTDNYIRVVSESREDIKGKIIETVLAELKEDYMTGRIY"
     gene            1878..2222
                     /locus_tag="PKI50_12355"
     CDS             1878..2222
                     /locus_tag="PKI50_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNT04524.1"
                     /translation="MKDCIFCRIIDGEIPCSMVYQDEKVLVFKDINPLAPVHVLIVSK
                     QHIESAQELNESSSEIAAHIFGKIPGIAKELGIAESGYRVVTNIGKDGGQAVPHLHFH
                     ILGGRKLENALG"
     gene            2401..2577
                     /gene="rpsU"
                     /locus_tag="PKI50_12360"
     CDS             2401..2577
                     /gene="rpsU"
                     /locus_tag="PKI50_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNT04525.1"
                     /translation="MSEIRVGENETLESALRRFKKKCARAGVLAEVRKREHYESPSVK
                     RRKKSEAARKRKTK"
     gene            2594..3040
                     /locus_tag="PKI50_12365"
     CDS             2594..3040
                     /locus_tag="PKI50_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNT04526.1"
                     /translation="MSLKEVLQEDLKSAMREKDVIRKNVITMVRAGILQIEKDKKIEL
                     DDEGVIEVIAKGVKQRKDSIPDFEKGNRPDLVENINKEIGILMGYLPQQLTEEELNQI
                     VSDTIFEVGAQSMQDLGKVMSALMPKVKGKADGKLVNSIVRQHLQR"
BASE COUNT         1066 a          499 c          825 g          749 t
ORIGIN      
        1 ttattattca aaagtgcact gaaatgggta ttaagagaat cgttcccgta cttacttcca
       61 gaactgtagt aaggcttgaa tccgaaaagg atgagaggaa gaaagcagaa agatgggcta
      121 agatagctga agaagccgca aagcaaagcg gcagagggat aatccctgtt ataggcatgc
      181 ctgtgactct ggatcaggcc ctaaggtctg cagccgggct ggatcttgta attataccct
      241 acgaactgga gactagccta cctgttaagg aagtattaag caaaaacaaa gccggaagca
      301 taggcttttt cataggcccg gaagggggct tcgagcctgt cgaggtggaa aaagcaaaag
      361 agatgggagc agtaccggta acgttgggca gaaggatact tcgcacggaa acggcgggaa
      421 ttgcggtact cacaagcata atgtacgagt atgatcagat gaagtaggag gaaatccatt
      481 gaaaaaggcg gcattttata cacttggctg caaggtcaac cagtatgaga cggaagcaat
      541 gacagagagc tttgagaatg caggctacga gattgtagat tattccgaat ttgcggatgt
      601 atatataata aacacctgca cagtaaccaa tatgggagac aggaaatcca gacagattat
      661 aagaagagcc agggaaaaga accctgaagc ccttgttgct gtcgtgggtt gctattcgca
      721 gatagcacct ggagaagtgc ttgagatacc ggaagtaagc ctggtagtcg gcacggatga
      781 acgcagcaga atggtagaat tggttgaata cgcaatggaa aaagaagaaa agcttaatat
      841 ggttaatgac attatgaaag taaaagaatt tgaggagatg tccataaaga gctacaagag
      901 cagaacgagg gcatttctta aaatacagga gggctgcgac cagtactgta catattgcat
      961 aattccatat gccagggggc atattaggag ccgcaaaccg gacagtataa tagccgaagt
     1021 aaaagagttg gcggagaacg gctttaggga gatagtgctg acgggaatac atgttgcttc
     1081 ttacggaaag gacctgggga atacttcttt gattgatata attgaaaagg ttcatgaaat
     1141 agacggaatc cggcgcattc ggatgagttc cgtagatcct aatgtaatga ctgacggatt
     1201 cattgaaagg ctcagccgcc ttccgaaaat atgcgggcat ttccatcttt ctcttcaaag
     1261 cggatgtgat gagaccctta agaggatgaa cagaaagtac acaaccgggg aatacagaag
     1321 ggtagtggaa aagctgagag aagtatttgc cgatgtggcg attacaacgg atttgattgt
     1381 gggatttccg ggagagacag aagaggagtt tcaaaaaacg gttgattttg ttgaggagat
     1441 agccttcagc gccatgcatg ttttcaagta ttcacaaaga tccggcactc ctgcagcaaa
     1501 gtatgagaat cagataaagc cgcaggtgaa ggattcgaga agcaaggtaa taaccgcaat
     1561 tgcccaaaaa aatgaagaaa aattcaaaaa agcctttatc gggcgcagca agccggttct
     1621 gtatgaacag ccgtttgatg gtcaaaaatc tctttttgaa ggcttgacag ataattatat
     1681 aagggttgta tctgaatcac gggaagacat aaagggaaaa ataattgaaa cagttcttgc
     1741 ggaattaaaa gaagattata tgaccggaag aatatattaa tttatttatt tgtgcaggat
     1801 ttaacggaat tttgttgaat aacatttagt acgccgtatt atattgcaag atcagactga
     1861 actaaggagg tgaagtgatg aaagactgta ttttttgcag aattatagat ggagaaatac
     1921 cttgcagcat ggtttatcag gatgaaaaag tattggtttt taaagatatc aacccacttg
     1981 ctccggttca tgtattgatt gtatcaaagc agcatattga gtctgcccag gaattgaatg
     2041 agagcagctc agagattgcg gcacatattt tcggcaaaat tccgggtata gcaaaggaat
     2101 taggtatagc ggaaagcggc taccgagtag ttaccaatat cggaaaagat ggcgggcagg
     2161 cagttcctca tctgcacttt catatattgg gcggccgcaa gcttgaaaat gctttgggat
     2221 agcctgagcc aaaaacccgt tattgacact aattaatcaa gtaatgtata ataaactgag
     2281 tattttatcg tattcagaag agtaaactga tggatatgat atggttttga tatgtcccac
     2341 ttagtaacgc agtatagcaa tatactgtaa ggattgttac cagcggaggg agggaaatgt
     2401 atgtctgaaa ttagagttgg tgaaaacgaa actcttgaaa gcgctcttag aaggttcaaa
     2461 aagaaatgtg ccagggctgg ggttttggca gaggttagga aaagagagca ttatgagagc
     2521 ccaagtgtaa aaagaaggaa gaaatctgaa gctgcaagaa aaagaaaaac aaaataaaag
     2581 aaggttgatg tgaatgtccc tcaaggaagt attacaggaa gaccttaagt ctgcaatgag
     2641 agaaaaagat gttataagga aaaatgttat aaccatggtg agagccggta tattgcagat
     2701 tgaaaaggac aagaaaatag agctggatga tgagggtgtt atagaggtaa ttgccaaagg
     2761 agttaaacaa aggaaggatt cgatacctga ctttgaaaaa ggcaacaggc ccgacctggt
     2821 tgaaaatatt aataaagaaa ttggtatact gatgggttat ttgccgcagc agcttaccga
     2881 ggaagaactc aaccaaatag tttcagatac gatttttgaa gtaggcgctc agagcatgca
     2941 ggatttaggc aaggtgatga gtgcacttat gcccaaggta aagggcaagg ctgacggcaa
     3001 attggttaat tcaattgtaa gacagcattt acagagataa aaaagacatg gggacggaag
     3061 acatggggac gtttcgcgtg tcccactgga agtgggacag gggaaacgtc cccatgtctt
     3121 ccgtccccat gtctttttt
//
LOCUS       DAOQUR010000118         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_38348_length_3001_cov_14.156144, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000118 DAOQUR010000000
VERSION     DAOQUR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38348_length_3001_cov_14.156144"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1639
                     /gene="thrC"
                     /locus_tag="PKI50_12370"
     CDS             146..1639
                     /gene="thrC"
                     /locus_tag="PKI50_12370"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT04527.1"
                     /translation="MNKIMYKSTRGRGGLITASAAILKGIAEDGGLFVPTEIPRPEIS
                     LEKMINMDYKSLAYHIMKDYFQNFEEAEIRECIDKAYDEKFDTPEIAPVVKKSGVYFL
                     ELFHGPTLAFKDMALSILPYFLKTAAKKQKLDSEIVILTATSGDTGKAALEGFAGVPG
                     TKIIVFFPESGVSEVQKLQMVTQTGENTYVIGIRGNFDDAQTGVKEMFSDKKLQMDMS
                     AANFVFSSANSINIGRLVPQIVYYFYAYLQLCRMGDIKAGEEINFTVPTGNFGNILAG
                     YHAKKMGLPVRHLICASNENKVLYDFLNTGVYDKKRKFITTMSPSMDILVSSNLERLL
                     FYLYGEDSSMVGELMAQLSGKGAYMINDNVKDKLNDFYGYYASEADTAEAIRKMYEDC
                     GYVIDTHTAVALSAYGKHRKRKCDENIKTVIVSTASPYKFTSDVMKSIDPKYSGQNSF
                     QLIKEMSRLTGAEIPKGIRDLDSRPVIHNTVCNKDEMKKQAEKILGL"
     gene            complement(1640..2341)
                     /locus_tag="PKI50_12375"
     CDS             complement(1640..2341)
                     /locus_tag="PKI50_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT04528.1"
                     /translation="MNENAKRILVVDDEEKIAEVVESYLTKSGYKVYTAYNGSNALEL
                     FERINPDLIILDLMLPDMSGEDICRSIRKKSRVPIIMLTARVEEESILTGLGIGADDY
                     VTKPFSPRQLTARVMALLRRSGDDIIPLSDVLSYNDGELLIDSIRHEVKRGGKPVNLT
                     PNEYKILMALVKYPQKAFTRDELVFLVLGEDYEGYDRTIDTHVKNLRQKIEPDAKSPK
                     YILTVYGVGYKFGGE"
     gene            complement(2379..2990)
                     /locus_tag="PKI50_12380"
     CDS             complement(2379..2990)
                     /locus_tag="PKI50_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNT04529.1"
                     /translation="MDILSIVLIAVGLSMDAFAVSVTNGIIIRDLRIGHALKVGLYFG
                     VFQALMPLIGWIAGSQFKEYIVSIDHWIAFGLLAFIGGKMIREACGEDKEEGVEGMCE
                     VAVSNQDTACENPLSMGRLIVLAVATSIDALAVGISFAFLRVSIVQSAVLIGAITFAI
                     CFAGVYIGHKCGCLMKKKAEKVGGFILICIGLKILFEHLGILG"
BASE COUNT          909 a          690 c          591 g          811 t
ORIGIN      
        1 agcgggacag gagaaacgtc cccgtgtccc cgggagaaac gtccccgtgt cctacttctg
       61 cttctgtgac gataattttt ttcacgatat atatatgtta taattaataa aatatatttg
      121 attttcaacc ggcggaggta aagcaatgaa taaaataatg tacaagagta ccagaggaag
      181 aggtgggctg attactgcat cggccgccat acttaaaggc atcgcagaag atggaggcct
      241 ttttgtcccg actgaaatac caaggcccga aatttccctg gaaaagatga taaatatgga
      301 ctacaaaagc ctggcatacc acattatgaa ggattacttt cagaattttg aagaagctga
      361 aatacgcgaa tgtattgata aagcctatga tgaaaaattc gataccccgg aaattgcacc
      421 tgtagtaaaa aaatcaggcg tgtattttct ggagcttttt cacgggccaa cccttgcctt
      481 caaggacatg gcattgtcca tactcccgta ttttctaaaa accgctgcca aaaagcagaa
      541 gctggatagc gaaatcgtaa tacttactgc tacctcggga gacaccggga aagctgccct
      601 ggaaggcttt gccggagtgc ccggcactaa aattatcgtg tttttcccgg aaagcggagt
      661 cagtgaagtt cagaaattgc agatggttac ccagacaggc gaaaatacat atgtaatagg
      721 catacgagga aattttgacg atgctcagac cggtgtgaag gaaatgttct ccgacaaaaa
      781 gctgcaaatg gatatgtcag ccgcaaactt cgtattttct tccgccaact ccataaatat
      841 aggcaggctg gttccccaga tagtgtatta tttttatgct tatctgcagc tttgcagaat
      901 gggtgacatt aaagccggtg aagaaatcaa ctttaccgta cccaccggaa acttcggcaa
      961 tatactggca ggatatcatg cgaaaaaaat ggggctccct gtcaggcacc ttatatgtgc
     1021 ttcaaacgaa aacaaggtgc tgtatgattt cctgaacacg ggagtatatg ataaaaagcg
     1081 aaaattcatc acaaccatgt ccccctcaat ggatatactt gtttccagca atctggaaag
     1141 gcttctgttt tatctttacg gtgaagacag cagtatggtg ggcgaattaa tggcacaatt
     1201 gtcaggaaaa ggtgcataca tgataaatga caatgtgaaa gataaactaa atgattttta
     1261 cggatattat gcatctgaag cagacactgc ggaagcaata agaaaaatgt atgaagactg
     1321 cggatatgtt attgacactc atacagccgt tgctctttca gcatacggaa aacacaggaa
     1381 gcggaaatgc gatgaaaata taaaaactgt gattgtttca acagccagcc cctataaatt
     1441 tacgtcagac gtaatgaaat caatagatcc caagtactct ggccaaaaca gcttccagct
     1501 gataaaagag atgagcagac ttaccggggc agaaattcca aagggtatca gggatctgga
     1561 cagcaggcct gtaattcata atactgtttg caataaagat gaaatgaaga aacaggcaga
     1621 aaaaattctc ggcttatgat tattcaccgc cgaacttata gcctacacca taaacagtca
     1681 atatgtattt tggtgatttt gcatccggtt caatcttttg gcggagattc ttcacatggg
     1741 tatctatggt tctgtcgtag ccttcataat cttcgcccag cacaaggaaa acaagctcat
     1801 cacgggtaaa agccttttga gggtatttca ccagggccat taaaatcttg tattcatttg
     1861 gagtaaggtt aacaggcttt ccgcctcttt ttacctcatg tctgatactg tctatcagca
     1921 gctccccatc attgtaggac agcacatcgg acaggggtat tatatcatcc ccggagcgtc
     1981 ttaaaagagc catgaccctt gctgtcaatt gacgcgggct gaagggtttt gtaacataat
     2041 catcagctcc tattccaagg ccggtcagta tgctttcctc ttccaccctt gcagtaagca
     2101 ttattatcgg tactcttgat ttttttctta tactcctgca aatatcctcg ccggacatgt
     2161 caggaagcat caaatccaat attatcagat cgggatttat cctctcaaaa agctccagag
     2221 cattgctccc gttataggct gtataaactt tataaccgct tttcgtcaaa tatgattcaa
     2281 ctacttctgc aatcttctct tcatcgtcaa ccacaagtat cctttttgca ttttcgttca
     2341 cggcaatccc tcccttatgg gcattataac ataacatact accctaatat tcccaagtgc
     2401 tcaaaaagaa tcttcagccc gatacatatc agtatgaatc ctccgacttt ctcagctttc
     2461 tttttcatca agcagccgca cttgtgtcct atatatactc ctgcaaagca tattgcaaag
     2521 gttattgctc caatcaaaac cgcagattgg actattgaaa ctctcaaaaa tgcaaagctt
     2581 ataccgaccg ccagagcatc tatgcttgta gccacggcaa gaacaatcag tctgcccatg
     2641 ctcagcggat tctcacatgc tgtatcctga tttgatacgg ccacctcgca cattccctca
     2701 acgccttctt ctttatcctc cccgcaagcc tcccgtatca tctttcctcc tatgaaggcc
     2761 aacaaaccaa aggctatcca atggtcgata cttactatgt attctttgaa ctgggaacct
     2821 gcaatccagc ctattaaagg catcaaggcc tgaaacaccc cgaaatacaa gccgactttc
     2881 aatgcatgtc caattctaag atccctgata atgattccat tggttaccga tactgcgaag
     2941 gcgtccatcg aaaggccaac cgcaatcaaa acaattgata gtatatccat gtttctcctc
     3001 c
//
LOCUS       DAOQUR010000119         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_39097_length_2961_cov_9.220922, whole genome shotgun sequence.
ACCESSION   DAOQUR010000119 DAOQUR010000000
VERSION     DAOQUR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39097_length_2961_cov_9.220922"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKI50_12385"
     CDS             complement(<1..352)
                     /locus_tag="PKI50_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNT04530.1"
                     /translation="MAVKVGIPRGMYFYKYYPFIQSFLTELGAEVVPSPETTKEILDK
                     GVLYSIDDACLPVKVLHGHAYSLRDKADCILIPRLTSVSKGEYICPKFGGLPEMVKYS
                     VPDLPPVIDTEINLI"
     gene            complement(504..1034)
                     /locus_tag="PKI50_12390"
     CDS             complement(504..1034)
                     /locus_tag="PKI50_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNT04531.1"
                     /translation="MTALLELFLVFLKIGAFSFGGGYAVLALIQKEVIENSGWISPKE
                     FIDIVAIAEMTPGPIAVNSSTFVGYRVGAVAGSAIATLGVVLIPITISLIVSVYYNKF
                     KHLKQVNRIIKGIRPAVLGLIAAACIKIAWASIVDIKGIIIALLTFYGVWKLKINPII
                     AILASGVLGVIFYGVI"
     gene            complement(1031..1558)
                     /locus_tag="PKI50_12395"
     CDS             complement(1031..1558)
                     /locus_tag="PKI50_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNT04532.1"
                     /translation="MDIFLTFFKIGSFTIGGGYAMLPIIQKEVVDNKKWLGEEEFLDS
                     IAVTNSLPGPLAINCATFVGYKTAGYAGAAAAALGAVLPSFLIILVIAVFFASFRENP
                     IVERVFSGIRPAVVALIAYALFKLVKSMGVNPLNISFSIAVLLLILLFNFHPIVTIVI
                     CGALGFFLYRKEEKQ"
     gene            complement(1569..2153)
                     /locus_tag="PKI50_12400"
     CDS             complement(1569..2153)
                     /locus_tag="PKI50_12400"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNT04533.1"
                     /translation="MKDTLRRQVLQARNKLYPEEVFSLSEKVFQNLIKNSFFNNSAHI
                     MVYLDFKNEVKTDLIINYCLENNKKVYVPICIPETHELCISRIINLEELQSGHFGIRE
                     PRPEYIRLSDSSLVDLVLVPGVAFDSTGNRIGFGAGYYDRFMRRLEPGAIKAALAYSF
                     QIADIIPSGQYDIPMDYIVTEKRTISCTASNKPR"
     gene            2309..2806
                     /locus_tag="PKI50_12405"
     CDS             2309..2806
                     /locus_tag="PKI50_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNT04534.1"
                     /translation="MNRNSNIVIGAVLITMGVLFLMSNLGYLNFSWNYIWPLALLVPG
                     IYMHFAFFTGIDKNPGILVPGGILTTYGALFYANVFFGWHIMADLWPLFLIGIAVGLF
                     ELYLFGNRDKGLLIPVGILGGIGLSSLLRVYMRFDIKNYLVPILLIIIGIVIITRKDN
                     RRSNG"
BASE COUNT          854 a          608 c          628 g          871 t
ORIGIN      
        1 ttataaggtt tatttcggta tcaattacag gaggcagatc cggaaccgaa tatttcacca
       61 tttccggaag tcctccgaat ttggggcata tatattcacc ctttgatacg cttgtaagtc
      121 gtggtatcag gatgcaatcc gccttatctc taaggctgta tgcatgaccg tgaagtacct
      181 tgacgggaag gcacgcatcg tctatactgt acagtacgcc cttatcaagt atttcctttg
      241 tggtctcggg agacggtaca acttccgccc ccagttcggt aaggaaggac tgtatgaaag
      301 gataatactt ataaaaatac attccccggg gaatgcctac tttgacagcc actgaatcac
      361 ctctttccat gaagtatatc gtgataattt tttttcacgg catatatatt attgacaaaa
      421 gaacagaggt ttaaaacaaa aataaaaaac cggaaagctt aatgacatga tcccgggttt
      481 tggatgcagt ttgatgcttt tatctaaata accccgtaga atataactcc gagaactcct
      541 gatgcaagta tagcaataat cggatttatc ttaagcttcc agactccata aaaagtcagc
      601 aaagcgatta taattccctt gatatcgaca attgaagccc atgcgatttt tatgcaggca
      661 gcggcaataa gccccaatac tgcgggccgt attcctttaa tgatccggtt gacctgcttc
      721 aggtgtttga atttattata gtaaaccgac acaatcagtg aaattgttat ggggatcaga
      781 actactccga gggtggcaat ggctgaccct gcaacagcac cgaccctgta gcccacaaag
      841 gttgatgaat taacggcaat cggccccgga gtcatttccg ctatggcaac tatatcaata
      901 aattccttcg gtgaaatcca gccgctgttc tcgattactt ctttctgtat cagtgccagt
      961 actgcatatc ctcccccgaa gctgaaggcc ccgattttca ggaagaccag aaaaagctcc
     1021 aataatgcag tcattgtttt tcctcctttc tatagaggaa aaatccgagg gcgccgcata
     1081 ttactatagt tactatagga tgaaaattga aaagtaaaat aagcagcagc accgcaatgg
     1141 agaaggatat gtttaaggga tttacaccca ttgatttaac aagcttgaac aaagcataag
     1201 caataagcgc aactacagcc ggccttatcc ctgaaaatac acgctcgaca atcgggtttt
     1261 ccctgaaaga tgcaaaaaac acagctatta caagtattat caggaaggag ggcaggactg
     1321 ctccaagggc ggctgcagcc gctcctgcat agcctgctgt tttgtagccg acaaaggtag
     1381 cgcagtttat ggctaaaggg cccggaaggc tgttggttac tgcaatggaa tccagaaatt
     1441 cttcctcacc aagccatttt ttgttgtcga caacttcttt ttgaataatg ggaagcattg
     1501 catagcctcc tcctattgta aaggagccta ttttgaaaaa tgtcaggaaa atatccatca
     1561 gcatagtatc acctcggttt gttgctggca gtgcagctta tggtgcgttt ctcagttact
     1621 atataatcca tggggatgtc gtattggcct gaaggaataa tatccgcaat ctgaaaggaa
     1681 taagcaagag cagccttaat ggcccccggt tcaaggcgtc tcataaacct gtcataatag
     1741 cctgccccga agcctattct gtttccggta ctgtcaaagg ctactcccgg aacgagtacc
     1801 agatccacaa ggctgctgtc tgacagcctg atgtactccg gccttggctc ccgtatgccg
     1861 aaatggcccg attgaagctc ctcaaggttt ataatccttg aaatgcagag ttcatgagtt
     1921 tccggtatgc aaataggcac atatactttc ttattattct caaggcaata atttattatt
     1981 aaatcagtct ttacctcatt cttaaaatca agatatacca ttatgtgggc tgaattgttg
     2041 aaaaaactgt tttttattaa attctggaaa actttttcac tgagggaaaa tacctcttcc
     2101 gggtataatt tgttcctggc ttgcagcact tgtctcctta aagtatcctt catgtgttcc
     2161 tccatatgta tcttagggat tttcatagta attatagtac tattattaat ataacagagg
     2221 taaatatgaa tttccatcag acatttgcct gaggcctccg tatattagtt agaaggtaat
     2281 tatggaagcg cagttgagga ggaatattat gaatagaaat tctaatatag taatcggagc
     2341 ggttttaata acaatgggag tattgtttct catgtcaaat cttggctatc tgaatttttc
     2401 atggaactat atatggccat tggcactgct ggttcccgga atatatatgc attttgcatt
     2461 ttttacgggt attgacaaga accccggcat acttgtaccg gggggcatac tgactacgta
     2521 tggagcgctg ttctatgcca atgttttttt cggatggcac attatggctg atctttggcc
     2581 gttatttctg ataggcattg cagtggggct ttttgagctg taccttttcg gaaaccgtga
     2641 caaggggctg cttataccgg tgggtatcct tggagggata ggactttcat cattattgag
     2701 ggtatatatg agatttgaca ttaagaatta tctggttccc attttactga taataatcgg
     2761 aatagttata attacaagaa aggacaacag gagaagcaac ggttaaaaaa ccagcaggcc
     2821 aaaaccaata gggcttcggg cagttggaag ccatgttgcg tttataagtg ccggctgttt
     2881 acatatatac tctttcataa ccaataaagg ctgctgaata taatgccagg gttttactta
     2941 tgtttaatcc gatgcattat a
//
LOCUS       DAOQUR010000120         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_42380_length_2807_cov_10.819041, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000120 DAOQUR010000000
VERSION     DAOQUR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42380_length_2807_cov_10.819041"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1085
                     /locus_tag="PKI50_12410"
     CDS             <1..>1085
                     /locus_tag="PKI50_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT04535.1"
                     /translation="AGFLEKLEDWREKSLYLSTDELIWYLYNDTGYYSFVGAMPGGAA
                     RQANLRMLFDKARQYEESSFKGLFNFINFINKLKGNQGDLGSAKVLGEKDDVVRIMSI
                     HKSKGLEFPVVFLCGAGKGFNLTDTAKNILLHHELGIGPDFIDHKRRIWYPSIFKQAL
                     KYKIKLENLSEEMRILYVAFTRAKEKLIITGAVKDIARSTDRWKSSSGANTQKVPEFD
                     VLRGRSFLDWICTALMRDNEGKPAERLESVEGWDIRFWNREEVLRGAVVEEHGKEELI
                     DEAALSSAEANMKDEIIKRLEWKYPYSAYEKLPVKVTVTELKRYFDTEAADEFRMQAA
                     PGIRKRPRFLEETSGMTAAERGSLMHFV"
     assembly_gap    1086..1185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1186..1389
                     /locus_tag="PKI50_12415"
     CDS             <1186..1389
                     /locus_tag="PKI50_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04536.1"
                     /translation="DCWFEEEGKLVLVDYKTDYVPEGEVSRIKEKYRTQIEYYARALS
                     QITGKEVSGKYIYLFWNGKILEY"
     gene            1493..2218
                     /gene="sigI"
                     /locus_tag="PKI50_12420"
     CDS             1493..2218
                     /gene="sigI"
                     /locus_tag="PKI50_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HNT04537.1"
                     /translation="MFYKSINERVIGIKLNRDEKDAFIEEYKPFIAAAVEKATGRYVS
                     YGYDEELSIGLMAFDEAIIHYDQEKGHFLSFAQGIIRKRLIDYYRKEKKHQAVTYVSE
                     YSTDEADGEEVFEYVIAAPEAQNKYYLEEINQLRRQELVQLKEELNNWGLEFSDVAKS
                     SPKHTNTKSSYLDIVKYIIDRPELVSKIKEKKYLPIADIEEGLKLPRKTIERSRNYVI
                     AAMIILTGDYYCIREFIDWGWKK"
     gene            2215..>2807
                     /locus_tag="PKI50_12425"
     CDS             2215..>2807
                     /locus_tag="PKI50_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HNT04538.1"
                     /translation="MKAIIAEIDKKHMIVITDKGDFVKVKRQMSAAIGDEIELKQRRI
                     YPISKHLAGLAACFMACIFLSTGVYAYCTPYSYVSVDINPSLSLALNRFERVISVNPL
                     TEDAVDLIKDAKSLKNRNIDAALSEIIKTASDKGYINEEAEDEIVVIVSAKNPKQEKK
                     LEETVAKTAEKELSKVNNAGGVTVEKTNVENHKTAIS"
BASE COUNT          893 a          450 c          714 g          650 t
ORIGIN      
        1 gcgggatttt tagaaaaact ggaggattgg agggaaaaat ccctttatct ctccaccgat
       61 gaattgatat ggtatttata caatgatacg ggatattaca gctttgtcgg agcgatgccg
      121 ggaggggctg cccggcaggc caacctcagg atgctttttg acaaggcaag gcagtatgag
      181 gagtcaagct ttaagggtct tttcaatttt atcaacttta taaacaagct gaaggggaac
      241 cagggggatt tgggaagtgc aaaggttctt ggagaaaagg acgacgtggt aagaattatg
      301 agcatacata aaagcaaggg cctggagttt cccgttgtat ttttgtgcgg cgcggggaag
      361 ggcttcaacc ttacggacac cgcaaagaac atactgctcc atcacgaact gggtataggc
      421 cctgatttta ttgaccacaa aaggaggata tggtatcctt caatatttaa gcaggctttg
      481 aaatataaga taaagttgga gaacctctcg gaggaaatga ggatattgta tgtagccttt
      541 accagggcga aggaaaagct tataataacc ggagccgtga aagatatcgc caggagtact
      601 gaccgctgga aaagcagcag cggtgcaaat acacaaaaag tacctgagtt tgatgtatta
      661 aggggcagaa gctttttaga ctggatatgt acggctctta tgagggataa cgaaggaaaa
      721 ccagccgaaa ggcttgaaag tgttgaggga tgggatatca gattttggaa cagggaagag
      781 gtcttaaggg gagcagttgt tgaagagcat ggcaaggaag agcttataga tgaagcggct
      841 ctttcctccg ccgaagccaa tatgaaggat gaaataatta aaaggctgga gtggaaatat
      901 ccttattctg cttatgagaa actgccggtg aaggtcaccg ttacagagct aaagagatac
      961 tttgatactg aggcggctga cgagtttcgt atgcaggctg ccccgggaat cagaaagagg
     1021 ccccggttcc tggaggaaac atcaggaatg actgctgcgg agagaggttc tttaatgcat
     1081 tttgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngactg ctggtttgaa
     1201 gaagagggaa agctggtgct ggttgattac aagactgatt acgtgcccga aggagaagta
     1261 agcaggataa aagaaaaata caggacacag atcgaatact atgcgcgggc tctgagtcaa
     1321 ataaccggca aagaggtatc ggggaaatac atataccttt tttggaatgg aaaaatattg
     1381 gaatattagc cttttaccaa tatatgttcc gtgaagggct tgtccggata atgtataatt
     1441 gtacagagac acttttggga cgtatgcatg cgttcttctg ttgaggtgtg ccatgtttta
     1501 taaatccata aatgaaagag taataggtat aaaacttaat agagatgaaa aggatgcttt
     1561 catagaggaa tacaagccct ttatagctgc cgctgttgaa aaggctacgg gcaggtatgt
     1621 ctcttacgga tatgatgagg aactcagtat agggctcatg gcttttgacg aagccataat
     1681 tcattatgac caggagaaag ggcacttttt aagctttgcc cagggcataa ttcgaaaaag
     1741 actgattgat tactacagga aagaaaagaa gcatcaggca gttacatatg tcagtgaata
     1801 ctccaccgat gaagctgacg gagaagaagt tttcgagtat gttattgcag ctcccgaagc
     1861 tcagaataaa tactatctgg aggagataaa ccagctcaga agacaggagt tggtacaact
     1921 gaaggaagag ctcaataatt gggggcttga attttcagat gtggcaaaaa gctctcccaa
     1981 gcatacgaat acaaagagtt catacctgga tatagtgaag tacataatcg acaggccgga
     2041 gcttgtgtca aaaataaaag aaaaaaaata tcttcccata gcagatattg aagaaggact
     2101 aaaattaccc cggaaaacca tagaacgctc acgaaattat gtaatagcag caatgataat
     2161 tttgacggga gattattatt gtataaggga gttcatagac tgggggtgga aaaagtgaag
     2221 gctataatag cagaaataga taagaaacat atgatagtga taaccgacaa gggagacttc
     2281 gttaaagtta agagacagat gtcggctgca ataggagatg aaatagagct caagcagcgc
     2341 aggatatatc ccataagcaa acacctggcc ggattggctg cctgcttcat ggcatgcata
     2401 ttcctgtcaa cgggagtata tgcatattgc accccataca gctatgtaag cgtagatatt
     2461 aatcccagtt tgtccttggc cctgaacagg tttgaaaggg taatatcagt gaatcctctg
     2521 acggaagatg cggttgattt aattaaggat gcaaaaagcc tgaagaaccg gaatattgat
     2581 gctgcgctgt ccgagattat aaaaactgct tctgacaagg gctatataaa cgaagaggcg
     2641 gaagatgaaa tcgttgtcat tgtttccgcg aagaatccaa agcaggagaa gaagcttgag
     2701 gaaacggtcg caaagactgc ggaaaaggaa ttgtccaagg ttaataatgc cggcggagta
     2761 acggttgaaa agactaatgt ggagaatcat aagactgcaa tatccca
//
LOCUS       DAOQUR010000121         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_44566_length_2715_cov_10.926316, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000121 DAOQUR010000000
VERSION     DAOQUR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44566_length_2715_cov_10.926316"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..178)
                     /locus_tag="PKI50_12430"
                     /pseudo
     CDS             complement(101..178)
                     /locus_tag="PKI50_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005903443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(336..1052)
                     /locus_tag="PKI50_12435"
     CDS             complement(336..1052)
                     /locus_tag="PKI50_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04539.1"
                     /translation="MNNIYKVIKYQIHDFWKAVLIFYLIIILIASAITVTVSVNGENI
                     SFGGLEMATVIFLFVAGLNCFKSNFKFMLANNVSRKGFYIGNIIALVAVSAFMALVDA
                     VLNVILNNVLVSNIPYESVVMQLYGNNSFFAGFLWRFGLNTWAVCFGWFVTMLYYRCN
                     TIMKIVVSFLPVLAFIIYQHVDRILGGLADRAVIDFLTRAMGFAYNNNVYIGALSLIT
                     GSAVIALICLLPLRNAVAKG"
     gene            complement(1070..1930)
                     /locus_tag="PKI50_12440"
     CDS             complement(1070..1930)
                     /locus_tag="PKI50_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT04540.1"
                     /translation="MKTIQIKGVSKVYGQTKALDKVDITIESNRIYGLLGRNGAGKTT
                     LLNLVTNRIFPTEGEILIDGETVFENDRVLGNIFYMTEQNLYPEDERVRNIYKWTKEF
                     YPSFDMEYAKDLSERFGLDTKKRVKNLSTGYASIFKAIAALASNADIVLYDEPVLGLD
                     ANHRDMFYKELISNYSENPKTIIISTHLIEEVAPVIEEAIIIKEGKLILKRSVEELLS
                     EAYIISGEASKVDEYIQGRKCIDIDSIGSFKSATVLEAVNNKDEALAEKLELEFGKAE
                     LQKLFISLTN"
     gene            complement(1899..2294)
                     /locus_tag="PKI50_12445"
     CDS             complement(1899..2294)
                     /locus_tag="PKI50_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT04541.1"
                     /translation="MLIDFNSEKPIYLQLAEAIEDNILKGIFEEETQIVSTTEISVKY
                     KINPATAGKGVNVLVDEGILYKKRGLGMFVSTGAKEKILEKRRNSFYKEYVQSLLEEA
                     AKLSISKEDIIKMIVGGNGIEDNSDKGSK"
     gene            complement(2444..>2715)
                     /locus_tag="PKI50_12450"
     CDS             complement(2444..>2715)
                     /locus_tag="PKI50_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNT04542.1"
                     /translation="KGIVFGEVISGVNFIKDFAAGLSNIFGGRSGSYEGELIEAREAA
                     LREMEKRAGTIGANAIIGVDIDYEVLGQGGNMLMVTASGTAVVIE"
BASE COUNT          786 a          663 c          399 g          867 t
ORIGIN      
        1 agcattggct gcttaaatat cttgctgaga agtggaaacc aaaaggcctg atataggaat
       61 ttcccacaag cagcggggac ggttctttct gcttttcaga agtgtccact actccgtcta
      121 tacgggaaaa gtattcctcc acaccccaaa aacaccctcc ggcaagccat atttctttat
      181 tcataaaatc accttacctt ttcaatacgt tctaatatgt cagtatgctg cttatatcca
      241 tcaaacaggg cattgaatta tggattatat cagccttttc atcaagcttg ctttttactt
      301 ccttgaattg ttctgccatc tcactctgcc cttatctaac ccttagcaac tgcattacga
      361 agaggcaaaa ggcagattag tgcgattacg gctgatcctg taataaggct taaagcacca
      421 atatagacat tgttattgta agcaaagccc atagctctag ttagaaaatc tataactgcc
      481 ctgtctgcca atccacccaa tattctgtca acgtgctggt atatgataaa tgccagaaca
      541 ggtaaaaatg aaacaacgat tttcattata gtgttgcatc tatagtacaa catggttaca
      601 aaccatccaa agcagacagc ccatgtattt aaaccaaaac gccacaagaa ccctgcaaag
      661 aaggagttat ttccataaag ctgcataacg acactttcat aagggatatt cgaaacaagt
      721 acattattca atataacatt caatacagca tccaccaatg ccataaatgc cgatactgca
      781 accaaagcaa ttatgttgcc gatatagaat cctttcctcg atacattatt tgcaagcatg
      841 aatttaaaat ttgatttaaa gcaattcagc cctgccacaa aaagaaatat cactgttgcc
      901 atttccaggc ccccgaagga tatgttttcc ccattcaccg aaaccgtgac agtaattgca
      961 gatgctatta atatgataat taagtaaaat attagcacag ctttccaaaa atcatgtatc
     1021 tgatatttaa tcaccttata gatattattc atgactatcc cgcctccaac taattagtta
     1081 agcttataaa aagcttttga agttctgcct tgccaaactc cagttccaac ttctccgcca
     1141 gagcttcatc cttgttattc acggcttcca atacagtagc cgacttaaag cttccgatag
     1201 aatcaatatc tatacatttc ctgccttgaa tgtattcatc tacctttgaa gcttctcctg
     1261 aaattatata agcctctgac aaaagctctt ccactgaccg cttcagaatc agctttcctt
     1321 ctttaattat gattgcctct tcgattaccg gagccacttc ctcaataaga tgggtagata
     1381 taattatagt ctttggattt tcgctgtaat tggaaataag ttctttatag aacatatccc
     1441 tgtgattcgc atccagtccc agcaccggtt catcatacag gacaatatct gcattggagg
     1501 caagagctgc aatggccttg aatattgaag cataacccgt agataagttc tttacccttt
     1561 ttttcgtatc cagcccgaac ctttccgata aatctttagc atattccata tcaaaggagg
     1621 gataaaattc ctttgtccat ttatatatat tccttaccct ttcatcttcg ggatacaggt
     1681 tttgttcagt catataaaaa atattaccta ggacccgatc attttcaaaa actgtttctc
     1741 cgtcaattaa gatttcaccc tctgtcggaa atatcctgtt tgtcaccagg tttaaaagtg
     1801 ttgtcttgcc tgccccgttt cttcccaata acccgtaaat cctgtttgat tcaatggtaa
     1861 tatcaacctt gtcaagggcc tttgtttgcc cataaacctt acttactccc tttatctgaa
     1921 ttgtcttcaa ttccgttacc ccccactatc attttaatga tatcctcctt tgatatactc
     1981 agctttgcag cctcttcaag aagggactgc acatactcct tatagaagct gttccggcgc
     2041 ttctcgagaa tcttctcttt tgcccctgtg gaaacaaaca ttcccagccc cctcttttta
     2101 tagaggatac cttcatctac aagcacattt actccctttc ctgctgttgc cggatttatt
     2161 ttatatttta ccgatatttc agtggtagat actatttggg tttcttcctc aaaaatcccc
     2221 tttaatatat tgtcttcaat ggcttctgcc agctgcagat atatcggctt ttcactgtta
     2281 aaatcaatga gcatgaattc cagcctcctc tattagttag ttaactactt atgtaattaa
     2341 ctatataaca tatatgatac attgtcaaat aaaatcgtaa aaaaaagcac attcctgcaa
     2401 atcataacag aaatgtactc atattttctt cgtctttttc tttttactca atcaccaccg
     2461 ccgtacccga agcggttacc atcagcatat tgcccccctg ccccaatacc tcatagtcaa
     2521 tgtctacccc tatgattgca ttggctccaa tagtgcctgc tcttttctcc atttccctta
     2581 atgccgcttc tctagcctca atcagttctc cctcatagga tccggagcgt cctccgaaaa
     2641 tgtttgagag cccggcagcg aaatccttaa taaagtttac tccggatata acttctccga
     2701 atactattcc cttgt
//
LOCUS       DAOQUR010000122         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_45251_length_2688_cov_11.128371, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000122 DAOQUR010000000
VERSION     DAOQUR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45251_length_2688_cov_11.128371"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1158)
                     /locus_tag="PKI50_12455"
     CDS             complement(64..1158)
                     /locus_tag="PKI50_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT04543.1"
                     /translation="MKQLKHIIAFFFIIIIVLGFMACTPQIAEEHPRNPEENLQEPEE
                     PAEVEEPDEADEPKADPVDLKAIKANENGQVMILMYHGIGEKEAVWMRTAENFRKDLE
                     VLYNKGYRLISLKDLIENNIKVEAGYTPFVLTFDDGAENNFNIIEENGEKKIDPNCAV
                     GILEEFKKEHPDFGSGGSFFIYYPIPFRQKELIKEKYEFLIANGYDIGNHSYNHENLG
                     KLSIEGIQKSLAKNVKSTQEYLPDYDVYALALPNGIGPKGDDYKYAATGEYEGVKYNN
                     RAVLKVGSNPAPSPVNVKFDPIRLPRVRASEMETEGVGMYDWLQYFEKKPEKRYISDG
                     NPDTVAIPESEAEYVDKSRLNGKELVIYKE"
     gene            1343..1531
                     /locus_tag="PKI50_12460"
     CDS             1343..1531
                     /locus_tag="PKI50_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04544.1"
                     /translation="MSNRRSKFTNIAGNMVEFSRELILPQDRHFHREAKSQKVQQQGV
                     HRNGTNKGRKIPIKRKMT"
     gene            1742..1966
                     /locus_tag="PKI50_12465"
     CDS             1742..1966
                     /locus_tag="PKI50_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HNT04545.1"
                     /translation="MAAGSGKYNKGLKVPNAHKALDNMKYEIASELNLPVHQGSEDYW
                     GNLSAKDCGRVGGNMVKKLITMAEQQLEGN"
     gene            2101..2625
                     /locus_tag="PKI50_12470"
     CDS             2101..2625
                     /locus_tag="PKI50_12470"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HNT04546.1"
                     /translation="MISDSGALADKKLMILYLLSKVGIPLTKSQITNAILENNLIDFF
                     TFQQCISELEESGMIKHTQYQKRQCCKATDSGIRAVGIFEQRISKNTAQIINNYISKN
                     KESLRKESQVIAEYKKMDDKEYIVSLKVIENELVLIDLKLNVVSAKQAKQICEKWKNS
                     SENIYSQLIGSLIN"
BASE COUNT          824 a          566 c          497 g          801 t
ORIGIN      
        1 tgtcccactt ccagtgggac acgcgaaacg tccccatgtc cctgtcccca tgtccccgtc
       61 gtattattct ttatatatca ctaactcttt cccgttcagc ctcgatttgt ccacatactc
      121 cgcttcgctt tccggtattg ctacagtatc cggatttccg tcgcttatat aacgcttttc
      181 cggcttcttt tcaaaatact gaagccagtc atacataccc acgccctctg tttccatttc
      241 gcttgccctc accctgggga gcctgatagg gtcaaactta acatttacgg gagacggtgc
      301 gggattgctt cctaccttga gcactgccct gttattatat tttactccct catattcacc
      361 cgtggctgca tatttgtagt catccccctt gggccctata ccgttgggaa gcgcaagggc
      421 atatacatca taatccggca gatattcctg cgtgcttttc acattctttg ccaaggattt
      481 ttgaatacct tcaatgctga gctttccaag gttttcgtga ttataggaat ggttgccgat
      541 atcatagccg tttgcaataa ggaactcgta tttctcctta ataagctcct tctgtctgaa
      601 gggaatggga taatatataa agaagctgcc tccgctgcca aagtcgggat gctccttttt
      661 aaactcctca agtattccta cggcgcaatt agggtcaatt tttttctctc cgttttcttc
      721 gataatatta aaattatttt ctgctccatc atcaaaagtg agcacaaaag gcgtgtatcc
      781 cgcttcaact tttatattgt tttcaataag gtctttaagg cttataagcc tgtatccctt
      841 attgtaaagc acctccagat cctttctgaa gttttccgct gttctcatcc atacagcttc
      901 tttttctccg atgccatgat acatgagtat cattacctgg ccgttctcat tagccttaat
      961 cgcctttaaa tctaccgggt cagcttttgg ttcgtcagcc tcatcaggct cttcaacctc
     1021 tgcaggctct tcaggctcct gcaaattttc ttccggattc cggggatgtt cttcagctat
     1081 ctgaggtgtg catgccataa aaccaagtac aataattatt ataaaaaaaa atgcaataat
     1141 gtgcttcaat tgtttcattt catgctcctt tcggtatctt actgctctat actaatatag
     1201 attataccag aattccccgc agctttcgac attatgcgcc aataaagcgg gtgttatttt
     1261 ttcccatata ccgcaagcaa aattcagaaa taaaattttg ggcaaaatat atttgtactt
     1321 ttttatttcg gaggtggaaa atatgtcgaa caggcgcagc aaatttacaa atattgcagg
     1381 aaatatggtt gaattctcca gagaattgat attgccgcag gacagacact tccacagaga
     1441 agcaaaatca caaaaagtac agcagcaggg tgtgcacaga aacggaacaa ataagggaag
     1501 aaagatcccc atcaaaagaa aaatgacgta atacttcaca caaacgggga caggagttca
     1561 ttctcctgtc cttgaattat ttttcctgaa actataatta tatgcatcaa actataatat
     1621 tattcataaa aacataatgt tctaacatac tttattgaca aaaaggttaa attaataatg
     1681 cctcacagaa aaaaaaagaa cacgttttcc attaatattt tattttaagg agggtttaag
     1741 aatggctgca ggaagcggaa aatacaataa agggctgaag gttccaaatg cccataaggc
     1801 tcttgataat atgaaatatg aaatagcttc agaattaaat ttgcctgtac accaaggctc
     1861 cgaagactat tggggaaatc tatcggcaaa ggactgcgga agagtcggag gaaatatggt
     1921 taagaagctt atcacaatgg cagagcaaca gcttgaaggc aattaattgc ctttcagcat
     1981 gttaaataaa caaaaatcag gctgcaacat gtctgatttt tgtttttgct tatccaaaat
     2041 caattatgct ataattaatt tgtctacttg gcagaactgc tgtttaaagg agatgatcgg
     2101 atgatttctg acagcggggc attggctgat aaaaagctta tgatattgta tctcttaagc
     2161 aaggtgggaa tacctctgac caaatctcag atcacaaacg ccattttaga aaacaacctt
     2221 atagactttt tcactttcca gcagtgtatt tcagagttgg aagagtccgg aatgataaag
     2281 catacccaat accagaaaag gcaatgctgt aaggctaccg acagcggcat cagagccgtc
     2341 ggaatctttg agcaaagaat atcaaaaaat accgcccaga taatcaataa ttacatatca
     2401 aaaaataagg aaagcctcag gaaggaatca caggtaattg cagaatataa aaagatggac
     2461 gataaagaat atatagtaag ccttaaggtt attgaaaacg agcttgtgct tattgatctc
     2521 aagcttaatg tcgtatctgc gaaacaagcg aaacaaatct gcgaaaagtg gaaaaactcc
     2581 tcagaaaaca tatacagcca actgattggc tctttaatca attaattttt ataggaggta
     2641 atatccatga aaattggtct ggttggtctg cctcttgtag gcaaaaca
//
LOCUS       DAOQUR010000123         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_48968_length_2557_cov_10.701439, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000123 DAOQUR010000000
VERSION     DAOQUR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48968_length_2557_cov_10.701439"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /gene="gatB"
                     /locus_tag="PKI50_12475"
     CDS             <1..1232
                     /gene="gatB"
                     /locus_tag="PKI50_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNT04547.1"
                     /translation="QSRMDRKNYFYPDLTKAYQITQYDIPICRKGYVDIGTEVLSRRI
                     RLARIHLEEDAGKLIHPEDSDVSLIDYNRAGVPLIEIVTEPDLRSAEEAVDFLKALKS
                     MLEYGGISDCRMERGSLRCDANISLRRAGQNTLNTRVELKNLNSFKELQKALENEEER
                     QRELYSLGKENRLVQETRRWDGAKGKTVPMRSKEDAHDYRYFPEPDLGLVIISDAMIR
                     EIRESLPEMPADRKKRFRDLYGLKDKEIDIIVGDKALSEYYEALTARGTDPRTGANWI
                     LGSMLKLLKEKGLEAGQIPVSPEELRKLIKIIKEGKISLTAGQEVFSQMFDTGRSAGD
                     IIKEKGLSQISNRTELGGIIADVLAGNSQSLSDYAAGKTQAAVFLMGQIMKASKGRAN
                     PQLAREILEEKLKKGLP"
     gene            complement(1265..2452)
                     /locus_tag="PKI50_12480"
     CDS             complement(1265..2452)
                     /locus_tag="PKI50_12480"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT04548.1"
                     /translation="MVSKDMLHYGTVRSCIRELFEYGKQRSSVVGKENVYDFSLGNPS
                     VPAPEEVNQTIRNIIDGMPSTAIHGYTSGPGSNETRAAIINNLNKRFGTDYKFTDLYL
                     TAGAAAALTAAFRALTIDDKTEFIAFAPFFTEYSCFATTAGAKLTVIPADIPAFQINF
                     DLLEKSINGNTQGVIVNSPNNPSGVIYTEETIRKLAALLKKKSAEVGHPIFIISDEPY
                     RELVYEGTVPFIPHYYPNTIICYSYSKSLSLPGERIGYVLVPGCVEDHDDVYNAVAGA
                     GRMLGYVCAPSLMQHVIARCADVKPDLRPYIKNRELIYNGLTEIGYNCAKPSGAFYLF
                     MEAPNGDAKSFSEMAKQLDLLIVPGNDFGCPSYVRISYCVDYEMIKRSLPVFRKLMEK
                     IKG"
BASE COUNT          745 a          586 c          625 g          601 t
ORIGIN      
        1 cacaaagccg gatggaccgc aaaaattatt tttaccccga cctcacaaag gcatatcaga
       61 tcacacaata tgatattcct atctgcagaa aggggtatgt agacataggg acggaggtac
      121 tgtcccgcag aatcaggctg gccaggatac acctggaaga ggatgccgga aagcttatac
      181 atcctgaaga ttccgatgtt tcactgattg actataaccg cgccggggtt ccccttatag
      241 aaatagtcac cgaaccggat ctgcgttcgg cagaggaagc ggttgatttt ctcaaagctc
      301 tcaaatccat gctggaatac ggggggatct cagactgccg tatggagcgg ggctccctgc
      361 ggtgcgatgc aaatatctcc ctcagaagag caggccagaa tacactgaat acaagggtgg
      421 agctgaaaaa cctgaactcc ttcaaggaac tgcagaaggc ccttgaaaat gaggaagaac
      481 gccagagaga actttacagc cttgggaagg aaaacagatt agtgcaggaa acaaggcgat
      541 gggacggtgc gaaggggaaa accgttccca tgcgttcaaa ggaggatgcc catgattaca
      601 ggtatttccc cgagccggac cttgggctgg ttataataag tgatgcaatg atccgtgaga
      661 taagagagtc cctgccggaa atgccggcag acaggaaaaa acgcttcagg gacctctacg
      721 gactgaagga taaggagatt gacataatag taggagacaa ggcactctcc gaatactatg
      781 aagctctgac tgcccggggt acggatccaa ggacgggggc caactggatc ctgggaagca
      841 tgctgaagct gctgaaggaa aaagggctgg aagcggggca gatacctgtc tctcccgaag
      901 aactccgcaa gcttataaaa attattaagg aaggaaaaat aagcttaacc gccgggcagg
      961 aagtattcag ccaaatgttt gataccggca gaagcgccgg ggatataata aaagaaaaag
     1021 gtctcagcca aatcagcaac aggacggaat tgggaggaat catcgcggat gtattggctg
     1081 gcaattctca atcactttcg gactatgctg ccggaaaaac gcaggcagcc gtgtttttga
     1141 tggggcaaat tatgaaagcc tcaaaaggca gggcaaaccc gcagctggca agagaaatac
     1201 tggaggagaa gcttaagaaa gggctgccgt aaaattctta ttttatgcag cccttatttt
     1261 tttatcagcc ttttattttc tccatcagct ttctgaatac aggcagagag cgcttaatca
     1321 tctcgtaatc cacacagtaa gaaattctca cataagaggg gcatccgaaa tcgttaccgg
     1381 gtactataag cagatccaac tgctttgcca tttcggaaaa gctctttgca tccccattcg
     1441 gagcttccat gaaaagatag aaggctccgg agggttttgc acaattgtat cctatctcgg
     1501 tcagcccgtt gtatatcaat tcacgattct taatgtaagg cctgaggtcg ggcttcacat
     1561 ccgcacatcg tgcaataacg tgctgcataa gggaaggagc acatacgtag cccagcatgc
     1621 gtccggctcc cgcaacggca ttataaacat catcatgatc ttcaacacag cccggcacca
     1681 gcacatagcc gatacgctcc ccgggcagtg acagtgattt gctgtaagaa tagcagataa
     1741 ttgtattagg gtagtaatgg ggaataaagg gcaccgtccc ctcataaacc agctcacgat
     1801 aaggttcgtc gctgattatg aatatgggat ggcctacctc cgcagacttc ttcttaagca
     1861 gggccgccag cttcctgatt gtctcttccg tgtagataac accggaagga ttgttgggag
     1921 aattgacaat aacaccttgg gtattcccat taatactctt ttcaagcaag tcaaagttaa
     1981 tctgaaatgc gggtatgtcc gctggaatca ccgtaagctt ggcgcccgcc gtcgtcgcaa
     2041 agcaggaata ctctgtgaaa aaaggtgcaa aagcgatgaa ttccgtttta tcatctatgg
     2101 tcaatgcgcg aaatgccgca gtcagtgccg cagccgcacc tgccgttaaa taaaggtctg
     2161 taaacttata gtcggttcca aaacgcttat tcaggttatt gattatggct gctcgagtct
     2221 cattactgcc cggaccgctt gtatagccat ggatggctgt ggatggcatt ccatcaatga
     2281 tattccgtat ggtttgattg acttcctccg gggccggaac actgggattg ccaagactga
     2341 agtcatatac attttccttc cccacaaccg aactgcgctg ttttccatac tcaaacagct
     2401 cacggataca ggaacgaacc gttccataat gaagcatgtc tttagatacc atttcgaacc
     2461 tccaaaatac ttattttata tctggccgat gatacaggcc tgatattcac caaaacaatc
     2521 agttttatta aaaatttttc agcattgtta atttttt
//
LOCUS       DAOQUR010000124         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_50674_length_2502_cov_10.423376, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000124 DAOQUR010000000
VERSION     DAOQUR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50674_length_2502_cov_10.423376"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..>1278)
                     /locus_tag="PKI50_12485"
     CDS             complement(7..>1278)
                     /locus_tag="PKI50_12485"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT04549.1"
                     /translation="GRHILEIYPSLTEETSTILRVIKTGRPILNYEQTFKNYKGIQIT
                     TINSTIPIISGKRVMGALEVSRDITEVKKLSLKVVDLQAELMKDARPEKSYDGNKAFF
                     NFADIIGISEAMLKLKKDAMAVASSPSPIMVYGETGTGKELLLHAIHNASLRRDKPFI
                     AQNCAALPETLLESILFGTVKGSFTGAENRPGLFEIANGGTLFLDEINSMSLQLQAKL
                     LRVIQDGNVRRVGDTKTIHVDVRIVTAINIDPRQAVVDKTLRDDLFFRLSVISLKVPP
                     LRERKGDIHFLIEHFIKKYNKRLHKNVSHVSSSVEEFFLSYAWPGNVRELEHAIEGAM
                     NITEGDVIESKHLPHYLGEIFSKFDFTQEDHEIKSLKEALYNLERSLIEKALKRCEGN
                     VTRAAELLDIPRQTLQYKISKYIIKADNAGF"
     assembly_gap    1279..1378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1473..2468)
                     /locus_tag="PKI50_12490"
     CDS             complement(1473..2468)
                     /locus_tag="PKI50_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HNT04550.1"
                     /translation="MTKALALISGGLDSILAAKLIQMQGIEVIGVCFASAFFGSKNAE
                     KMAAQIDMPLIVVDFTEEHLKMTKDPKHGYGKNMNPCIDCHAMMLNYAGKMLEEYGAS
                     FLITGEVLNQRPMSQNKKSLGIVQKESGFEDKILRPLCALNLPPTKMEIDGLVDRSKL
                     LGISGKSRKQQIELARQWGIKDYPSPAGGCMLTEPQFANRLRDLYSHDKEAVGPMDIK
                     LLRIGRHMRISPSARIVCTRNEGEYEPLMELVKEEYLIFDTADCSGSTVVLIPSGAGV
                     LSDEDVLFAASVAARYSKEREKDSVRVKFKKKKDSEYEYIQVKPAEDEDIKKVLL"
BASE COUNT          556 a          610 c          475 g          761 t
ORIGIN      
        1 ttatgcctaa aatccggcat tgtcagcttt gatgatatac ttgcttattt tgtattgaag
       61 tgtctgtctt ggtatgtcca gcagctctgc tgcccttgtt acgttgcctt cgcacctttt
      121 cagggctttc tcaataaggc ttctttccag gttgtacagg gcttccttca gggactttat
      181 ttcatgatcc tcctgggtga agtcaaattt tgaaaatatc tctcccaggt aatggggaag
      241 gtgcttactc tcgattacat ccccctctgt tatgttcatg gctccttcta tggcatgctc
      301 cagttccctg acattccccg gccatgcata gctcagaaaa aactcctcca cgctgctgct
      361 tacatgactg acattcttat gcagccgttt gttgtatttc ttaataaaat gctctatcag
      421 gaagtgtatg tcacccttcc tttctcttaa aggaggcacc ttcagagata ttacgctcag
      481 acggaagaac aggtcatccc ttagagtctt atccacaacc gcctgcctgg gatcaatatt
      541 tatagccgtc actattctga catcaacgtg aatagttttt gtatcgccga ctcttctcac
      601 attgccgtcc tgtattaccc tcagcagctt tgcctgcagt tgaagagaca ttgaattgat
      661 ctcatccagg aacagggtac cgccattggc tatttcaaaa agccccgggc ggttttccgc
      721 tccggtgaag ctgcctttta ccgtaccgaa aagtatgctt tccagcaagg tttccggaag
      781 agccgcacaa ttttgggcaa tgaaaggttt gtccctgcgg aggcttgcat tatgtatggc
      841 atgaaggagc aattcctttc ctgtgcccgt ctctccataa accataatcg gtgaaggaga
      901 tgaggctact gccatggcat ctttcttgag cttgagcatt gcttccgata ttccgattat
      961 atctgcaaaa ttaaagaatg ccttatttcc gtcgtagctc ttttcaggcc ttgcatcttt
     1021 cataagctcc gcctgcaaat ccacaacctt caaggaaagc ttctttacct ccgttatgtc
     1081 cctcgatact tcaagagcac ccataacccg cttccccgaa ataatgggta tggttgaatt
     1141 tatggttgtt atctgaatgc ctttgtagtt cttaaaggtc tgctcatagt taagtatggg
     1201 ccttcccgtt tttataaccc tcagtattgt gctggtttcc tctgtcaggg agggatatat
     1261 ttcgagtatg tgcctgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     1381 aaaagcaggt tgattacctg ctatatctta agtatatacc acccgacaga aaattacaaa
     1441 taatctgttg aatttttaga aaattcatga atctacagca gcaccttctt tatgtcttcg
     1501 tcctcggcag gttttacttg tatgtattca tactcactgt cttttttctt tttgaatttc
     1561 acccttacgc tgtccttttc cctttctttg ctatacctgg ctgcaacgga agcagcaaac
     1621 aaaacatcct catcagacaa cacccctgcc ccggagggta tcagaacgac tgtggagccg
     1681 ctgcaatccg cagtatcaaa tatgagatat tcttctttca caagctccat taatggttca
     1741 tattcacctt cattcctggt gcatacaatc cgcgcagaag gggatatcct catatgcctg
     1801 ccgattctca ggagctttat atccattggc cccaccgctt ccttgtcatg gctgtacaaa
     1861 tctctcagac ggttggcaaa ctgcggttcg gtaagcatgc agcctccggc aggggaaggg
     1921 taatccttga tcccccactg tcttgccagc tctatctgct gttttcttga tttgccgctg
     1981 ataccgagaa gcttgctcct gtcaacaagg ccgtcaattt ccattttcgt cggcgggaga
     2041 ttcagagcac aaaggggcct cagtatcttg tcttcaaagc cggactcctt ttgcactatg
     2101 cccagggatt tcttattctg ggacattggc ctctggttaa gtacttctcc ggtgattaaa
     2161 aaacttgcac catattcctc aagcattttc cccgcatagt tcagcatcat tgcatggcag
     2221 tcgatgcaag ggttcatatt ctttccgtat ccatgcttgg gatcctttgt cattttcaga
     2281 tgctcttcgg taaaatcaac tactattaaa ggcatatcta tttgtgccgc cattttttcg
     2341 gcatttttgg agccgaaaaa cgcagaggca aagcacactc caattacttc aatcccttgc
     2401 atttgaatca gttttgcggc taatatgctg tcaagccccc cggaaatcag ggccaaagct
     2461 ttggtcatat gtatatcaat cctttccagt tcttacgctc tc
//
LOCUS       DAOQUR010000125         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_59239_length_2268_cov_12.310438, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000125 DAOQUR010000000
VERSION     DAOQUR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59239_length_2268_cov_12.310438"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKI50_12495"
     CDS             complement(<1..428)
                     /locus_tag="PKI50_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HNT04551.1"
                     /translation="MTLFSPWALWFLSFMPLVVLMYILKQKFEERQISSIYLWQQVLK
                     DIEVNTPWQKLKKNLLLFLQLLSILFLVFALSDPYLYTGGGMHSNLVIVIDNTGSMNA
                     RYENGTRLAKAKQLAEEMISRSGTKANITLLTVGRSPRVE"
     gene            complement(433..1311)
                     /locus_tag="PKI50_12500"
     CDS             complement(433..1311)
                     /locus_tag="PKI50_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT04552.1"
                     /translation="MGERIFDSDFLKKLDTIVINVRIMMNTGSGGNRKSRSKGSSVEF
                     SDFREYSLGDDFRRIDWNAYGRFDRLFVKLFMEEREAMVNIFVDSSRSMNFGEPKKSL
                     SALKLSGALAYLALNNLDRVCLNSLAGTLVKQSSVLSGRGMFRQCADFLENMQFDGVT
                     DLNSCIKKKEFKGSGVSIVFSDFFTPGGIEDAVKYLLYKKQDVILVHVLAPEELNPEL
                     EGQMRLLDSETGEARDISVTPAMLSQYDKELSLFMNNIREFCSRMGAAYIQVSSEEPI
                     EKIVLEEFTRAGIIGK"
     gene            complement(1324..>2268)
                     /locus_tag="PKI50_12505"
     CDS             complement(1324..>2268)
                     /locus_tag="PKI50_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT04553.1"
                     /translation="KAIIGQKDIVKQVLISILAGGNVLLEGVPGLGKTQLVKTLSKVM
                     ELSFSRIQFTPDLMPADVMGTNIIVKDDSGNSRFEFQKGPVFANLVLADEINRATPKT
                     QSALLEAMQEQTVTVGKVTYSLPQPFLVLATQNPIEMEGTYPLPEAQMDRFLFKLNVL
                     FPSFDELKGIMDITTSNKEVELNKIVDGESILEMRKIIREVPIAKPVQEYALRVVLGT
                     HPEIEGAPEGAKKYIRFGASPRAAQAIITASRARALMEGRYNVSFEDIKYVAHPSLRH
                     RFFLNFDAVADGITTDGLIDQLLDEISADIVPARHIKG"
BASE COUNT          581 a          612 c          439 g          636 t
ORIGIN      
        1 atttcaaccc tgggactcct gcccaccgtc aaaagggtta tattggcctt tgtccccgaa
       61 cggcttatca tctcttccgc cagctgcttt gctttagcca gtcttgtgcc attctcatac
      121 cttgcattca tgcttccggt attgtctatt acaattacca gattggaatg catacctccc
      181 ccggtatata gatagggatc ggaaagcgca aaaaccaaaa aaagaatcga taaaagctgc
      241 aaaaagagga gcaggttttt cttaagcttc tgccaagggg tattgacttc gatatccttc
      301 aatacctgct gccaaaggta gatgctcgaa atctgcctct cttcgaattt ctgcttcaat
      361 atgtacataa gcactacaag gggcataaag gataaaaacc acaatgccca aggtgaaaac
      421 agcgtcataa aatcacttcc caataatacc cgctctggta aattcctcaa gaactatctt
      481 ctctatgggt tcttccgaag aaacctgtat ataggccgct cccatcctgc tgcagaattc
      541 cctgatatta ttcataaata aactcagctc tttatcatac tgcgaaagca ttgccggagt
      601 aaccgatata tccctggctt caccggtttc actgtccagc agcctcatct gcccctccaa
      661 ttccggattc agttcttcag gtgccaatac atgaaccagg attacatcct gctttttata
      721 tagcaggtat tttaccgcat cctcaattcc tcccggggtg aaaaaatcgg aaaagactat
      781 tgagactccg cttcccttga actctttctt cttaatacag gaattcaaat cggtgacgcc
      841 gtcaaattgc atattctcca gaaaatctgc acattgccgg aacatccctc tgccgcttaa
      901 aactgaagac tgcttgacaa gtgttcctgc caagctgttc aggcatactc tgtccaggtt
      961 gttcaatgcc aggtaagcca gtgcccctga aagcttcaag gctgacaggg acttctttgg
     1021 ctctccgaaa ttcatggatc tgctgctgtc aacaaaaata ttgaccattg cctcccgctc
     1081 ttccatgaaa agcttgacaa aaagcctgtc gaacctgccg taggcattcc agtctatacg
     1141 ccggaaatcg tcccccaggg agtattccct gaaatcggaa aactccacgg aacttccctt
     1201 gctgcgggac ttcctgttgc ctcccgaacc ggtattcatc ataatcctta cgtttatcac
     1261 tattgtatca agttttttca aaaagtctga atcaaagatc cgttcaccca aatcaaacac
     1321 ccgctatccc ttgatatgcc ttgcaggcac aatatcagca cttatttcat ccaaaagctg
     1381 gtcaattaat ccgtctgtgg taattccgtc agccacggca tcgaagttca ggaagaacct
     1441 gtgcctcaga gaagggtgcg caacatattt aatatcttca aaggagacat tgtatctgcc
     1501 ttccatgaga gcccttgccc tggatgcggt aattattgcc tgagcggctc tcgggcttgc
     1561 cccaaaccga atatacttct ttgcaccttc aggtgcccct tcaatttcag gatgtgttcc
     1621 caataccacc ctgagggcat attcctgtac gggcttggct attggcactt ctcttataat
     1681 cttacgcatc tccagtattg actccccatc cacaatctta ttaagctcca cttccttatt
     1741 ggaagtcgtt atgtccatta tgcccttcag ttcatcgaag cttgggaaca aaacgtttaa
     1801 tttgaataga aaccggtcca tctgcgcttc gggaaggggg tatgtgcctt ccatctctat
     1861 gggattctgt gttgccagca ccaggaaagg ctggggaagg ctgtaggtta cttttcccac
     1921 cgtcaccgtc tgctcctgca tggcctccag aagtgcggac tgggtcttgg gagtagccct
     1981 gtttatttca tcagccagca caagattggc aaacaccggc cccttttgga actcgaaacg
     2041 gctgttgccg ctgtcatctt ttacaattat gtttgtcccc ataacatcgg caggcatcaa
     2101 atccggcgta aactgtatcc tggaaaagga cagctccatt actttagaca gggtctttac
     2161 caattgggtt tttccaagcc ccggaacccc ctcaagaagc acattccctc ctgccaggat
     2221 tgaaatcaat acctgcttta ctatatcctt ctgtccgatt attgcttt
//
LOCUS       DAOQUR010000126         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_67703_length_2086_cov_10.548991, whole genome shotgun
            sequence.
ACCESSION   DAOQUR010000126 DAOQUR010000000
VERSION     DAOQUR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67703_length_2086_cov_10.548991"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PKI50_12510"
     CDS             <1..757
                     /locus_tag="PKI50_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNT04554.1"
                     /translation="NETVAEHMYWKETPFIYRVHEDPDAEKLQAFNEFIFNFGYHLKG
                     IAEIHPKALQQLTDQIKGTKEERIINTLMLRSLKKARYTSESMGHFGLAAKYYCHFTS
                     PIRRYPDLMIHRIIKEDIHGKLTEKRLKHLHSIIESIAEQSSIRERAADEAEREVEDL
                     KKAEYMKDRIGEEYDGIISGVTSFGMFVELENTIEGLVHMSNMEDDYYQFDEMHHMLI
                     GERRRRTFRIGDSVRIKVLNADIANRTIDFSLV"
     gene            922..1386
                     /gene="smpB"
                     /locus_tag="PKI50_12515"
     CDS             922..1386
                     /gene="smpB"
                     /locus_tag="PKI50_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNT04555.1"
                     /translation="MAQVNIKTIAQNKKARHEYFIEDTYEAGIELSGTEVKSVRKGSV
                     NLKDSYAAIDKGQVYVYNMHISPYEQGNIFNKDPMRTRKLLLHKYEINKLIGYVQQKG
                     YTLIPLSIYLKGSLVKVQLAIAKGKELHDKRHDIAERDAKREMEREFRGKNY"
     gene            1391..1739
                     /gene="ssrA"
                     /locus_tag="PKI50_12520"
     tmRNA           1391..1739
                     /gene="ssrA"
                     /locus_tag="PKI50_12520"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1890..>2086)
                     /locus_tag="PKI50_12525"
     CDS             complement(1890..>2086)
                     /locus_tag="PKI50_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT04556.1"
                     /translation="GIIDITRLFRVVLKMKYIQGQDRSQITLFPDCIEDYVGEDNPVR
                     VIDAFVDNLDHAELGLSFLA"
BASE COUNT          686 a          386 c          471 g          543 t
ORIGIN      
        1 caatgagaca gttgcagagc atatgtattg gaaagagaca ccctttattt acagggtcca
       61 cgaggatccg gatgcagaaa agcttcaagc ctttaatgaa tttatattta actttggtta
      121 tcatctgaaa ggaatagctg agatacatcc aaaagcgctg cagcagctta ccgaccagat
      181 aaagggaacc aaagaggagc ggataataaa tacactgatg ctgcgttcgc tgaaaaaggc
      241 aagatatact tcggaaagca tgggacattt cggcctcgct gcaaagtatt actgccactt
      301 cacttctccg ataagaagat atcctgacct gatgatccac aggatcatta aggaggatat
      361 tcacggaaaa ctcactgaaa agaggctgaa gcatcttcac tcaataattg agagtatcgc
      421 tgagcaatcc tccatcaggg agcgggcggc agatgaagct gaaagagaag tagaagacct
      481 caaaaaggca gaatatatga aagacaggat cggagaggaa tatgacggaa taatttccgg
      541 cgtcacttcc tttggaatgt ttgttgaact ggaaaacact atagaaggct tggtgcatat
      601 gagcaatatg gaggatgatt actatcagtt tgatgagatg catcacatgc ttatcggtga
      661 aaggcggaga aggaccttca gaattggcga tagtgtaagg ataaaagtcc tgaatgctga
      721 tattgctaat agaacaatcg atttttcatt ggtttaaact gatttaacat tttttttcat
      781 tgatattaca gttgtatttc gttataatat ataagttgac tccggtacta atgatgttat
      841 aatattagtg catgctcata tttaaaatga aagcatataa cattttgaaa aaagcgtaat
      901 actttgtaaa gttggggtaa catggcacaa gtcaacatta agacaattgc acaaaataaa
      961 aaagccagac atgaatactt cattgaggat acctatgaag ccggtattga gctttcaggc
     1021 actgaagtga agtctgtaag gaaaggcagc gtaaatttga aggacagcta tgccgcaata
     1081 gataaagggc aagtttatgt ttacaatatg cacataagcc cgtatgagca gggaaatatt
     1141 ttcaacaagg atcctatgag gacaagaaaa ctgcttttgc acaaatatga aattaataag
     1201 ctcataggat atgttcagca aaaaggttat actcttatac cgctttcgat ttatcttaaa
     1261 ggcagcctgg ttaaggtgca gcttgcaata gcaaaaggca aggagctcca tgacaagcga
     1321 catgacatcg cagaacggga tgcaaaaaga gaaatggaaa gggaattcag aggcaaaaat
     1381 tattagacac gggggcgtaa tggtttcgac gggggtagtt gaggccgcgg cagcgagcgg
     1441 tggtcccaga ggcacctaaa aaagctggaa acttaaattt aaacgcagaa gataattttg
     1501 cattagcagc ctaagggctg ctcgttcaac ccggggttcc cgcaccttgg gattgggcgt
     1561 catttagcgg ggtacgtcac tgccaaagct ttgaggcagc tgtcgcatta tgaagctact
     1621 gaagctgtaa gcctgctttt gggcgtgcag cggagggaat gttaaagcaa aagctgcgct
     1681 cggagaagct gtgaccaaga tgctttcgga caggagttcg attctcctcg cctccaccat
     1741 attttgtccg aaaacccttg attttcaagg gtttaatttg ttttttgaag agaaaaacac
     1801 caacatattt aaagaaaaaa tcagggagcc tactgcatag ctgcaattaa ttccataatt
     1861 cccaccatat ttattgctct ttacagattc taggcaagaa aactaagccc cagttctgca
     1921 tgatcaaggt tatcaacaaa tgcatcaata acccttaccg ggttatcctc tccaacatag
     1981 tcttcaatgc aatcgggaaa cagtgtaatc tgtgatctgt cctggccttg aatatacttc
     2041 attttcagca ccaccctaaa taatcttgtt atatctatta taccat
//
LOCUS       DAOQUR010000127         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1109
            NODE_75178_length_1953_cov_9.486301, whole genome shotgun sequence.
ACCESSION   DAOQUR010000127 DAOQUR010000000
VERSION     DAOQUR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852452
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 11:55:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,507
            CDSs (total)                      :: 2,450
            Genes (coding)                    :: 2,426
            CDSs (with protein)               :: 2,426
            Genes (RNA)                       :: 57
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75178_length_1953_cov_9.486301"
                     /isolate="AMR_MDS_1109"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1170
                     /locus_tag="PKI50_12530"
     CDS             211..1170
                     /locus_tag="PKI50_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT04557.1"
                     /translation="MQRASGTEITTVRRQKPALLKELAKNKVLYLMLLPGLLVLLVNN
                     YLPMFGIVIAFKDYNYAKGFWKSDWVGFNNFEYLFNSKAAFLITRNTILYNVAFIVIA
                     ATVAVVLAIIFNELLNRRISKFYQSVMFLPYFFSWVVISYLVFALLSSDMGFVNKILI
                     SNGMQPVEWYVDPRFWPLIIILVNTWKWTGYDSIIYLAAIIGFDKSLYEAAAVDGASR
                     FQQITKVMVPMLMPVITVLTLIKVGRIFFTDFGLFFNIPRNSGPLYDVTNVIETYVFR
                     ALRQTGDIGMASAAGFYQAVVGFIVVMTANYIVRKIDKDSSLF"
     gene            1184..>1953
                     /locus_tag="PKI50_12535"
     CDS             1184..>1953
                     /locus_tag="PKI50_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT04558.1"
                     /translation="MRDFIKINRKADIALNIVMILCTIACVLPLLIVFSASITDEKTV
                     TMYGYSLFPRNLSLEAYKYIYKDIMVILRAYGVTVFVTVVGSALSLVTIALYAYPISR
                     KDFKYRNVFTFIVFLTMLFNGGLVPWYMVYVNVLHIDDTIMALILPYLMTPLYVLIMR
                     TFFMTTIPDGIIEAARIDGAGEFQTFVWIVLPLAKPVLATIGLFNILTYWNDWYAPLL
                     FINNEKLFNLQYLMYRVDKAIIYLSNNAGSINNATQIMA"
BASE COUNT          556 a          388 c          434 g          575 t
ORIGIN      
        1 gcaatatatc tcgattcgta cgatatgagc cgggatatat tgcattttta cgtcttttag
       61 tggaaaatgt tgcacttttg ttttgaacaa catagtaaaa caaattcaca agatgctcca
      121 tttaatggag agctgccgca gtatattatt ggggtatgaa gttaaacccg acaggatctg
      181 aaatagagga ggggtattcc tgtggctgaa atgcaaaggg cttcgggcac cgagataacg
      241 actgtgagaa ggcaaaaacc tgccttgctt aaagaactgg caaagaataa ggtgttgtac
      301 cttatgctgc ttcccggcct gctggtgctg ctggtcaata attaccttcc gatgtttggc
      361 attgttatag ccttcaagga ttacaactat gcaaaaggct tctggaaaag cgactgggta
      421 gggttcaaca attttgaata tcttttcaac tcaaaagcgg ctttcctgat aacaagaaac
      481 acaattctgt acaatgttgc attcatagta atagcagcaa ctgttgccgt tgtacttgcc
      541 attatcttca acgaactcct gaacaggcgc atatccaagt tctaccagag tgtgatgttt
      601 ctgccatact tcttttcatg ggttgtgata agctatctgg tgtttgccct cttaagctca
      661 gacatggggt ttgtcaacaa gattcttatc agtaacggta tgcagcctgt tgaatggtat
      721 gtagacccaa gattctggcc tttgataatt atactggtaa atacgtggaa atggacagga
      781 tacgacagca taatctatct ggctgcaatc atcggctttg acaaatcctt gtatgaagcg
      841 gcggcagttg acggcgcttc cagattccag cagataacca aagtgatggt tcccatgctt
      901 atgccggtaa ttaccgtatt aacactgata aaagtaggaa ggattttctt cactgacttc
      961 ggattgttct tcaatatacc caggaacagc ggcccgctgt atgatgtgac caatgttata
     1021 gaaacatatg tattcagggc gctcaggcag accggagaca tagggatggc atcggcagcc
     1081 gggttctatc aggcagtagt aggattcata gtagtgatga ccgcaaacta tattgtaaga
     1141 aaaatcgaca aagacagttc tttgttttaa ggaggcgtga gagttgaggg atttcataaa
     1201 aatcaaccgg aaagctgaca tagcgctcaa tattgtaatg atactctgta caatagcctg
     1261 tgtacttccg cttttaattg ttttctccgc ttccataact gatgagaaga cggttaccat
     1321 gtacggctac agcctttttc ccagaaacct gagcctggaa gcctacaagt atatttacaa
     1381 ggatataatg gtaattctga gggcttatgg agtgactgta tttgttactg tggtgggcag
     1441 tgcattaagc cttgttacca tagctttgta cgcatatccg atatccagaa aagactttaa
     1501 atacaggaat gtttttacat ttatagtttt tcttacgatg ctgttcaacg gaggccttgt
     1561 tccatggtat atggtatatg tgaatgtcct ccatatagat gacactataa tggcacttat
     1621 actgccctac ctgatgaccc ccctttatgt gctgattatg aggaccttct tcatgactac
     1681 cataccggac ggaataatcg aagctgcaag gattgacgga gccggtgagt tccagacctt
     1741 tgtatggata gtgcttcctc ttgccaaacc tgtattggcg acaatagggc tttttaatat
     1801 actcacttat tggaacgact ggtatgcacc actgctcttc ataaacaatg agaagctttt
     1861 caaccttcaa tacctgatgt acagggtgga taaagcgata atataccttt caaataatgc
     1921 aggctctata aacaatgcaa cccagatcat ggc
//
